LOCUS       DAZRVY010000001        88077 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZRVY010000001 DAZRVY010000000
VERSION     DAZRVY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.001"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXOX_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXOX_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR7003594.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHXOX_000002"
     tRNA            1230..1305
                     /locus_tag="ACHXOX_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHXOX_000003"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHXOX_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR7003595.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHXOX_000004"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHXOX_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR7003596.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHXOX_000005"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHXOX_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR7003597.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHXOX_000006"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHXOX_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR7003598.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHXOX_000007"
     CDS             complement(4868..5305)
                     /locus_tag="ACHXOX_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR7003599.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHXOX_000008"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHXOX_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHXOX_000009"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHXOX_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR7003600.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="ACHXOX_000010"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="ACHXOX_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR7003601.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="ACHXOX_000011"
                     /pseudo
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="ACHXOX_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="ACHXOX_000012"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="ACHXOX_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR7003602.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="ACHXOX_000013"
     CDS             10773..11441
                     /locus_tag="ACHXOX_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR7003603.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="ACHXOX_000014"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="ACHXOX_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR7003604.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="ACHXOX_000015"
     CDS             11984..13816
                     /locus_tag="ACHXOX_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR7003605.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="ACHXOX_000016"
     CDS             13806..14156
                     /locus_tag="ACHXOX_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR7003606.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHXOX_000017"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHXOX_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR7003607.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="ACHXOX_000018"
     CDS             14475..14897
                     /locus_tag="ACHXOX_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR7003608.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="ACHXOX_000019"
     CDS             14900..15532
                     /locus_tag="ACHXOX_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR7003609.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="ACHXOX_000020"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="ACHXOX_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR7003610.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="ACHXOX_000021"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="ACHXOX_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR7003611.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="ACHXOX_000022"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="ACHXOX_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR7003612.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="ACHXOX_000023"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="ACHXOX_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="ACHXOX_000024"
     CDS             18711..19823
                     /locus_tag="ACHXOX_000024"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR7003613.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19820..21163
                     /locus_tag="ACHXOX_000025"
     CDS             19820..21163
                     /locus_tag="ACHXOX_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR7003614.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21166..21720
                     /gene="pduT"
                     /locus_tag="ACHXOX_000026"
     CDS             21166..21720
                     /gene="pduT"
                     /locus_tag="ACHXOX_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR7003615.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21720..22070
                     /gene="pduU"
                     /locus_tag="ACHXOX_000027"
     CDS             21720..22070
                     /gene="pduU"
                     /locus_tag="ACHXOX_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR7003616.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22075..22518
                     /locus_tag="ACHXOX_000028"
     CDS             22075..22518
                     /locus_tag="ACHXOX_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR7003617.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22619..22948)
                     /locus_tag="ACHXOX_000029"
     CDS             complement(22619..22948)
                     /locus_tag="ACHXOX_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR7003618.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23120..24178)
                     /locus_tag="ACHXOX_000030"
     CDS             complement(23120..24178)
                     /locus_tag="ACHXOX_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR7003619.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHXOX_000031"
     CDS             complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHXOX_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR7003620.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHXOX_000032"
     CDS             complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHXOX_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR7003621.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHXOX_000033"
     CDS             26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHXOX_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR7003622.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHXOX_000034"
     CDS             complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHXOX_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR7003623.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHXOX_000035"
     CDS             complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHXOX_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR7003624.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29291..30649)
                     /gene="plaP"
                     /locus_tag="ACHXOX_000036"
     CDS             complement(29291..30649)
                     /gene="plaP"
                     /locus_tag="ACHXOX_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR7003625.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30639..30701)
                     /gene="yoeI"
                     /locus_tag="ACHXOX_000037"
     CDS             complement(30639..30701)
                     /gene="yoeI"
                     /locus_tag="ACHXOX_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR7003626.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30916..31845)
                     /locus_tag="ACHXOX_000038"
     CDS             complement(30916..31845)
                     /locus_tag="ACHXOX_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7003627.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31891..32715)
                     /locus_tag="ACHXOX_000039"
     CDS             complement(31891..32715)
                     /locus_tag="ACHXOX_000039"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7003628.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32798..33052)
                     /gene="yoeB"
                     /locus_tag="ACHXOX_000040"
     CDS             complement(32798..33052)
                     /gene="yoeB"
                     /locus_tag="ACHXOX_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR7003629.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33049..33300)
                     /gene="yefM"
                     /locus_tag="ACHXOX_000041"
     CDS             complement(33049..33300)
                     /gene="yefM"
                     /locus_tag="ACHXOX_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR7003630.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33583..33633
                     /gene="hisL"
                     /locus_tag="ACHXOX_000042"
     CDS             33583..33633
                     /gene="hisL"
                     /locus_tag="ACHXOX_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR7003631.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33609..33732
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33779..34678
                     /gene="hisG"
                     /locus_tag="ACHXOX_000043"
     CDS             33779..34678
                     /gene="hisG"
                     /locus_tag="ACHXOX_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR7003632.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34684..35988
                     /gene="hisD"
                     /locus_tag="ACHXOX_000044"
     CDS             34684..35988
                     /gene="hisD"
                     /locus_tag="ACHXOX_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR7003633.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35985..37055
                     /gene="hisC"
                     /locus_tag="ACHXOX_000045"
     CDS             35985..37055
                     /gene="hisC"
                     /locus_tag="ACHXOX_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR7003634.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37055..38122
                     /gene="hisB"
                     /locus_tag="ACHXOX_000046"
     CDS             37055..38122
                     /gene="hisB"
                     /locus_tag="ACHXOX_000046"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR7003635.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38122..38712
                     /gene="hisH"
                     /locus_tag="ACHXOX_000047"
     CDS             38122..38712
                     /gene="hisH"
                     /locus_tag="ACHXOX_000047"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR7003636.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38712..39449
                     /gene="hisA"
                     /locus_tag="ACHXOX_000048"
     CDS             38712..39449
                     /gene="hisA"
                     /locus_tag="ACHXOX_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR7003637.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39431..40207
                     /gene="hisF"
                     /locus_tag="ACHXOX_000049"
     CDS             39431..40207
                     /gene="hisF"
                     /locus_tag="ACHXOX_000049"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR7003638.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40201..40812
                     /gene="hisIE"
                     /locus_tag="ACHXOX_000050"
     CDS             40201..40812
                     /gene="hisIE"
                     /locus_tag="ACHXOX_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR7003639.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40909..41886)
                     /gene="wzzB"
                     /locus_tag="ACHXOX_000051"
     CDS             complement(40909..41886)
                     /gene="wzzB"
                     /locus_tag="ACHXOX_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR7003640.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42032..43198)
                     /gene="ugd"
                     /locus_tag="ACHXOX_000052"
     CDS             complement(42032..43198)
                     /gene="ugd"
                     /locus_tag="ACHXOX_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR7003641.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43448..44854)
                     /gene="gndA"
                     /locus_tag="ACHXOX_000053"
     CDS             complement(43448..44854)
                     /gene="gndA"
                     /locus_tag="ACHXOX_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR7003642.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45018..46388)
                     /gene="cpsG"
                     /locus_tag="ACHXOX_000054"
                     /gene_synonym="manB"
     CDS             complement(45018..46388)
                     /gene="cpsG"
                     /locus_tag="ACHXOX_000054"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR7003643.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46493..47929)
                     /gene="cpsB"
                     /locus_tag="ACHXOX_000055"
     CDS             complement(46493..47929)
                     /gene="cpsB"
                     /locus_tag="ACHXOX_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR7003644.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47932..49155)
                     /gene="wcaI"
                     /locus_tag="ACHXOX_000056"
     CDS             complement(47932..49155)
                     /gene="wcaI"
                     /locus_tag="ACHXOX_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR7003645.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49152..49631)
                     /gene="gmm"
                     /locus_tag="ACHXOX_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49152..49631)
                     /gene="gmm"
                     /locus_tag="ACHXOX_000057"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR7003646.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49634..50599)
                     /gene="fcl"
                     /locus_tag="ACHXOX_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49634..50599)
                     /gene="fcl"
                     /locus_tag="ACHXOX_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR7003647.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50602..51723)
                     /gene="gmd"
                     /locus_tag="ACHXOX_000059"
     CDS             complement(50602..51723)
                     /gene="gmd"
                     /locus_tag="ACHXOX_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR7003648.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51749..52297)
                     /gene="wcaF"
                     /locus_tag="ACHXOX_000060"
     CDS             complement(51749..52297)
                     /gene="wcaF"
                     /locus_tag="ACHXOX_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR7003649.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52313..53059)
                     /gene="wcaE"
                     /locus_tag="ACHXOX_000061"
     CDS             complement(52313..53059)
                     /gene="wcaE"
                     /locus_tag="ACHXOX_000061"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR7003650.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     CYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53070..54287)
                     /gene="wcaD"
                     /locus_tag="ACHXOX_000062"
     CDS             complement(53070..54287)
                     /gene="wcaD"
                     /locus_tag="ACHXOX_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR7003651.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54262..55479)
                     /gene="wcaC"
                     /locus_tag="ACHXOX_000063"
     CDS             complement(54262..55479)
                     /gene="wcaC"
                     /locus_tag="ACHXOX_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR7003652.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55476..55964)
                     /gene="wcaB"
                     /locus_tag="ACHXOX_000064"
     CDS             complement(55476..55964)
                     /gene="wcaB"
                     /locus_tag="ACHXOX_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR7003653.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55967..56806)
                     /gene="wcaA"
                     /locus_tag="ACHXOX_000065"
     CDS             complement(55967..56806)
                     /gene="wcaA"
                     /locus_tag="ACHXOX_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR7003654.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56899..59061)
                     /gene="wzc"
                     /locus_tag="ACHXOX_000066"
     CDS             complement(56899..59061)
                     /gene="wzc"
                     /locus_tag="ACHXOX_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR7003655.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59064..59507)
                     /gene="wzb"
                     /locus_tag="ACHXOX_000067"
     CDS             complement(59064..59507)
                     /gene="wzb"
                     /locus_tag="ACHXOX_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR7003656.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59513..60652)
                     /gene="wza"
                     /locus_tag="ACHXOX_000068"
     CDS             complement(59513..60652)
                     /gene="wza"
                     /locus_tag="ACHXOX_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR7003657.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60961..61110
                     /locus_tag="ACHXOX_000069"
     CDS             60961..61110
                     /locus_tag="ACHXOX_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7003658.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61311..62894
                     /locus_tag="ACHXOX_000070"
     CDS             61311..62894
                     /locus_tag="ACHXOX_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR7003659.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63168..65021)
                     /gene="asmA"
                     /locus_tag="ACHXOX_000071"
     CDS             complement(63168..65021)
                     /gene="asmA"
                     /locus_tag="ACHXOX_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR7003660.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65043..65624)
                     /gene="dcd"
                     /locus_tag="ACHXOX_000072"
     CDS             complement(65043..65624)
                     /gene="dcd"
                     /locus_tag="ACHXOX_000072"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR7003661.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65716..66357)
                     /gene="udk"
                     /locus_tag="ACHXOX_000073"
     CDS             complement(65716..66357)
                     /gene="udk"
                     /locus_tag="ACHXOX_000073"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR7003662.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66675..69992
                     /locus_tag="ACHXOX_000074"
     CDS             66675..69992
                     /locus_tag="ACHXOX_000074"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR7003663.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70102..70950)
                     /gene="alkA"
                     /locus_tag="ACHXOX_000075"
     CDS             complement(70102..70950)
                     /gene="alkA"
                     /locus_tag="ACHXOX_000075"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR7003664.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71084..72436
                     /gene="yegD"
                     /locus_tag="ACHXOX_000076"
     CDS             71084..72436
                     /gene="yegD"
                     /locus_tag="ACHXOX_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7003665.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72449..74389)
                     /gene="yegI"
                     /locus_tag="ACHXOX_000077"
     CDS             complement(72449..74389)
                     /gene="yegI"
                     /locus_tag="ACHXOX_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR7003666.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVVWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74386..75147)
                     /gene="pphC"
                     /locus_tag="ACHXOX_000078"
     CDS             complement(74386..75147)
                     /gene="pphC"
                     /locus_tag="ACHXOX_000078"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR7003667.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75144..75803)
                     /locus_tag="ACHXOX_000079"
     CDS             complement(75144..75803)
                     /locus_tag="ACHXOX_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR7003668.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76024..76083)
                     /gene="ibsA"
                     /locus_tag="ACHXOX_000080"
     CDS             complement(76024..76083)
                     /gene="ibsA"
                     /locus_tag="ACHXOX_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR7003669.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76356..76412)
                     /locus_tag="ACHXOX_000081"
     CDS             complement(76356..76412)
                     /locus_tag="ACHXOX_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7003670.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76683..76739)
                     /locus_tag="ACHXOX_000082"
     CDS             complement(76683..76739)
                     /locus_tag="ACHXOX_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7003671.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77018..78259
                     /gene="mdtA"
                     /locus_tag="ACHXOX_000083"
     CDS             77018..78259
                     /gene="mdtA"
                     /locus_tag="ACHXOX_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR7003672.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78259..81381
                     /gene="mdtB"
                     /locus_tag="ACHXOX_000084"
     CDS             78259..81381
                     /gene="mdtB"
                     /locus_tag="ACHXOX_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR7003673.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81382..84459
                     /gene="mdtC"
                     /locus_tag="ACHXOX_000085"
     CDS             81382..84459
                     /gene="mdtC"
                     /locus_tag="ACHXOX_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR7003674.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84460..85875
                     /locus_tag="ACHXOX_000086"
     CDS             84460..85875
                     /locus_tag="ACHXOX_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7003675.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85872..87275
                     /gene="baeS"
                     /locus_tag="ACHXOX_000087"
     CDS             85872..87275
                     /gene="baeS"
                     /locus_tag="ACHXOX_000087"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR7003676.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87272..87994
                     /gene="baeR"
                     /locus_tag="ACHXOX_000088"
     CDS             87272..87994
                     /gene="baeR"
                     /locus_tag="ACHXOX_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR7003677.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21551 a        23314 c        22152 g        21060 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgattaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcga ggagttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc
    20701 cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct gcgccagtgt
    20761 cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg
    20821 cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg caaattatcg
    20881 actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt atcaggatgc
    20941 gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat
    21001 cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt
    21061 aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg atggcatggt
    21121 cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc gaaagcggta
    21181 ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa
    21241 agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt tctgctgatg
    21301 ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt
    21361 gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct ttctgcaatc
    21421 agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc
    21481 gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat tcttgttcgc
    21541 gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg cgatatttca
    21601 gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg tttgttggtc
    21661 tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa
    21721 tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt aagcaggtca
    21781 cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg ggtttaccag
    21841 acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct atcatcgcat
    21901 gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg
    21961 ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag gttacccgta
    22021 cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa
    22081 cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt
    22141 ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat
    22201 acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt cagtgcctgc
    22261 gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc tttttcgcca
    22321 ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc agatttagcc
    22381 aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg
    22441 ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt gctgcataca
    22501 gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg gtgatacaca
    22561 gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt
    22621 acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc
    22681 gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac
    22741 ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc ttgatgtaat
    22801 cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt
    22861 cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt
    22921 cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga gagtcagatt
    22981 cgtttaagca accgctattc gttagggcca acattactca tagctgccgc agatgacaat
    23041 gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga
    23101 tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact
    23161 caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta
    23221 ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt
    23281 tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaagctca
    23341 gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga
    23401 aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat
    23461 tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg
    23521 cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt
    23581 ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact
    23641 cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac
    23701 cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg
    23761 tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca atcaataatc
    23821 cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc
    23881 agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga cctaaaatcg
    23941 aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta ccccagaacg
    24001 agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa
    24061 acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacta
    24121 tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc
    24181 agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca gaaggcgcgc
    24241 ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg agcgaaaaaa
    24301 ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg cgttaacaca
    24361 ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca
    24421 gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc
    24481 ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc
    24541 accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac
    24601 gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg tgtcgcagcg
    24661 taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc actccttcgg
    24721 cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct ttaccgtctg
    24781 ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct gcgtaatctc
    24841 gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc aatattaaaa
    24901 ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa
    24961 agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg ccgccttccc
    25021 cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt
    25081 caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg
    25141 tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt
    25201 ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt
    25261 tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc
    25321 ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca atgagacgac
    25381 gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt
    25441 tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt tgctgggtga
    25501 taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga
    25561 tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca
    25621 gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca
    25681 tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt
    25741 catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct
    25801 ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc
    25861 gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga
    25921 cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg
    25981 cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca tgaatctctg
    26041 gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga
    26101 acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata
    26161 gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac
    26221 taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt gtggcgctaa
    26281 agctgattag cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc
    26341 taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg
    26401 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat
    26461 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac tatttacccc
    26521 agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa
    26581 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
    26641 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
    26701 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttgctggatg
    26761 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
    26821 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg
    26881 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
    26941 gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
    27001 tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt ggggcatggc
    27061 gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc aatgccgtaa
    27121 ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc gacacattgc
    27181 gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
    27241 tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg
    27301 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt ctgcgtgaaa
    27361 atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt
    27421 cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat cgtgcagcaa
    27481 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg
    27541 ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
    27601 attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg cccgagtttt
    27661 tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat aacaaagaga
    27721 aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct
    27781 ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt gatactccac
    27841 gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc cgcccactgt
    27901 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
    27961 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
    28021 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
    28081 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
    28141 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
    28201 gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
    28261 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
    28321 aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
    28381 gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa
    28441 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
    28501 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cagttcggcg
    28561 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
    28621 cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag tctcagcaat
    28681 agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga
    28741 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
    28801 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
    28861 gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt cgtaagtcag
    28921 taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg caatcagcaa
    28981 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca
    29041 aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat
    29101 cattgaaaac ataaattaat taaccagatg aatgttaatg aggaaattat tcatgactgg
    29161 tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt aatgaggatt
    29221 tcgataaaaa aaaataaaaa aaccggagcc aaatattcat tcgactccgg ttgattatta
    29281 gaaaagatga ttatgcaacg tcttcgtact gcggaactgg gttacggaag ctcttggtga
    29341 cgcaagccag gtaaatcaga ccgatagcag cccaaatcag acccagaacc attgagcttt
    29401 cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc aggaacagat
    29461 actggaagtg atctttcagc gtcttattac gcttctcacg gatccagaac tgcgagatga
    29521 ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc gctgtagcca
    29581 ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg atgttcatcg
    29641 ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaat acgccgtcac
    29701 gacccattac gtacatcaga cgcgctacgc ctgcatgcgc cgccataccg gacgccagta
    29761 cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca acgtacagca
    29821 tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg aagtacagct
    29881 gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc aggaaaatcg
    29941 cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg atgccgtcaa
    30001 agccggtaaa ggaaaagcac aggattgtcg ccccggtaat catcggaata acatgcgcat
    30061 cgccagacca gaacggacga gtgctcgcca gcgtaccagc gccttcacct tcaaatacgc
    30121 cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg acgattacag
    30181 tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata aaggccacca
    30241 gcgccaccac aaacatccac gatgggatgg aaggcaccag agcttcaaaa tagattttcg
    30301 ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac caacccacca
    30361 taaagccgac agtcgggcta atggatttct gggcgtaagt atatgcagag ccagcagaag
    30421 gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca atcaatgcga
    30481 acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca aaagtatcaa
    30541 acagcgtcat cggctgcata taggcaagac ccatcattac aaccggaact aacgtaagcg
    30601 ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg gtcattctcc
    30661 tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga ttcctcagcg
    30721 acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc tcttggtatg
    30781 aatgattggt ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt ttagtcattt
    30841 tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg agaagcttgt
    30901 cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat aaacaccggc
    30961 gatcgcacta tttgctaaaa tttcatcccg ccaccagccg ctggcaagcc ctgcggtctg
    31021 ttcgttccag ccaatccaga cgggttcaaa gagagtttgg gcgaccactt tcttctcgat
    31081 taacgcgcca ctatcgagaa aacgttgcac cagataacgg ggtaaatagc cgcagccgag
    31141 gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg cttcttgttc
    31201 gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca ccacaatcgc
    31261 ccgatatcgc ttaatgatac gacggtttaa cggctcttct tcttgggcta acggatgatg
    31321 aggcgcgacc gcaaagactt gttccagatc gcccaaccgc gagaaaccaa actcactgga
    31381 agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag tcagggcatc
    31441 ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat gatgttgata
    31501 aaaagcttca atcagtggcg caagcagaga aaaaggaaac gtgtcgtcca cgccaatcac
    31561 cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt ctcgcacggt
    31621 atgcagaact tcccgcccct tctctaataa catttttccg gtacgggtga atttagcgcg
    31681 gtgaccgcta cgatcaagta attgaatatt aaggtcgctt tccagcttgt gaacggtgta
    31741 actcagagcg gacggcgtct tgtagagttt agccgacgcc gccgcaaaac tgccttcttt
    31801 ttctaaggca tcgaggatca tcaacacatc cagcagttgt ttcatacacg cccccttgca
    31861 gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca gcggatcggg
    31921 atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa tcttaccttt
    31981 gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac gggccatctg
    32041 cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga tgtgtccgcc
    32101 tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat ggactaaatt
    32161 aacaccatgt tcaccatcag gcgcggtttt tccggcaaag aagcgtccgg gatgacgtcc
    32221 cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta aattgtgcag
    32281 ccagtcttcg agttcttcta atactcgtcc actgttggtc accggattac gcggggtggt
    32341 ttctttcacc gtgccttgcg cgtcgccata gacagacgtt gagctggtaa aaataatgcg
    32401 aggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta aatagaactc
    32461 atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg catccatcag
    32521 ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca gatagctatc
    32581 aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc cggtgacttg
    32641 ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta acccgacaat
    32701 tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc tacgctagcg
    32761 tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa cgacatgctg
    32821 caatgagcag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct gtaatgcgtc
    32881 gggaccagaa acctgacaaa ttatgtttca ggggttctgg cttcccctta ccttcaaatg
    32941 gcgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc ttatctgttt
    33001 cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc actcaataat
    33061 gtccttttct gttcctttgc ctgatttcag gctatcgatt gagtccatca atctccgggc
    33121 gttagcgggg gagcgcagta gataagccgt ctcttccagc gagttgtatt cttcgagtga
    33181 catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat gatcttcaac
    33241 ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa ttgtacgcat
    33301 gtcaatctcc tcttttgtac agtttattgt acaatgatgg gcgttaatta actatttatt
    33361 aattagtttg tagatcaagg tattgtcagt gagacgaaaa tccaggcttc gctatttttg
    33421 gtgccgtcag cttagaggac agtcctctta gccccctcct ttccccgctc attcattaaa
    33481 caaatccatt gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta
    33541 ggttaaaaga catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt
    33601 aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac
    33661 attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg
    33721 gggctttttt attgcgcggt tgataacggt tcagacaggt ttaaagagga ataacaaaat
    33781 gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc
    33841 acgcgaatta ctggcgcgct gtggcattaa aattaatctt cacacccagc gcctgatcgc
    33901 gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct
    33961 ggtaatggat ggcgtggtag accttgggat tatcggcgaa aacgtgctgg aagaagagct
    34021 gcttaaccgc cgcgcccagg gtgaagatcc gcgctacttt accttgcgtc gtctggattt
    34081 cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa gcctgggacg gcccgctctc
    34141 cttaaacggt aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt atctcgacca
    34201 gaaaggcatc tcttttaaat cctgcttact gaacggttct gttgaagtcg ccccgcgtgc
    34261 cggactggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagcaaacgg
    34321 cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc cttatccagc gcgatggtga
    34381 aatggaagaa tccaaacagc agctgatcga caaactgctg acccgtattc agggtgtgat
    34441 ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgtc tggatgaagt
    34501 catcgccctg ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg gtgaccaaca
    34561 acgcgtagcg atgcacatgg tcagtagcga aaccctgttc tgggaaacga tggaaaaact
    34621 gaaagcgctg ggtgccagtt caattctggt cctgccgatt gagaagatga tggagtgatc
    34681 gccatgagct ttaacacaat cattgactgg aatagctgta ctgcggagca acaacgccag
    34741 ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcactgt taacgatatt
    34801 ctcgataacg tgaaagcacg cggtgatgac gccctgcggg aatacagcgc gaagtttgat
    34861 aaaaccacgg ttaacgcgct gaaggtgtct gctgaggaga ttgccgccgc tagcgaacgc
    34921 ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa aaaatattga aaccttccac
    34981 actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg ttgccagcag
    35041 gtcacgcgcc cggtcgcttc agttgggttg tatattcctg gcggctccgc cccgctcttc
    35101 tcaacggtat taatgctggc gactccggcg cgtattgcgg gctgtaaaaa agtggtgctg
    35161 tgctcaccgc cgccgattgc cgatgagatc ctttatgcgg cgcagctgtg cggtgtgaag
    35221 gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct
    35281 gtgccgaaag tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga agcgaaacgt
    35341 caggtgagcc agcgtctgga cggtgcggct atcgatatgc ccgcaggccc gtcggaagtg
    35401 ttggtcattg ccgacagcgg cgcaacgccg gatttcgtgg cttctgattt gctttctcag
    35461 gctgaacacg gcccggactc acaggtgatt ttactgacgc ctgacgctga tatggcgcat
    35521 caagttgccg aagccgtcga acgccagtta gcagaactgc cgcgtgccga aaccgcccgc
    35581 caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtagagatc
    35641 tccaaccagt acggcccgga gcacctgatc atccagaccc gcaacgcccg cgatctggtc
    35701 gatggcatca ccagcgccgg ttcggtattt cttggtgact ggtcaccgga atcggcaggt
    35761 gattacgcct ccggcaccaa ccacgttctg ccgacttacg gttacaccgc cacctgttcc
    35821 agcctcgggc tggcagattt ccagaagcgt atgaccgtgc aggaactgtc gaaagaaggt
    35881 ttctccgcgc tggcttcgac cattgaaaca ttggccgccg ccgagcgcct gaccgcccat
    35941 aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta
    36001 ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc
    36061 tgggcggtaa tggcgacgtc tggctgaacg ccaacgaata ccccactgcc gtggagtttc
    36121 agcttactca gcaaacgctc aaccgctacc cggaatgtca gccgaaagcg gtgattgaaa
    36181 attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagtcgt ggcgcggacg
    36241 aaggtattga actgctgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact
    36301 gcccgccaac gtacggcatg tacagcgtca gcgccgaaac gattggcgtc gagtgccgca
    36361 cagtgccgac gcaggacaac tggcaactgg acttacaggg catttccgac aagctggacg
    36421 gcgtaaaagt ggtctatgtt tgcagcccca acaacccgac agggcaactg atcaatccgc
    36481 aggattttcg cactctgctg gagctaaccc gcggaaaagc cattgtggtt gccgatgaag
    36541 cctatatcga gttttgcccg caggcatcgc tggctggctg gctgacggaa tatccgcacc
    36601 tggctatttt gcgcacactg tcgaaagctt ttgctctggc gggccttcgt tgcggattta
    36661 cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt gatcgccccc tacccgctct
    36721 cgacgccggt tgccgacatt gcggcccagg cgttaagccc gcagggaatc gtcgctatgc
    36781 gcgaacgagt gacgcaaatt attgcagaac gcgaatacct gattgccgca ctgaaagaga
    36841 tcccctgcgt agagcaggtt ttcgactccg caaccaacta cattctggcg cgctttaaag
    36901 cctccagcgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata
    36961 aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc
    37021 gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga
    37081 tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa
    37141 actcgccttt gaaccaggcg tgatcccgga actgctgaag ctgcaaaaag cgggctacaa
    37201 actggtaatg atcaccaatc aggatggtct gggaacacaa agtttcccgc aggcggattt
    37261 cgatggcccg cacaacctga tgatgcagat cttcacctcg caaggcgtac agtttgatga
    37321 agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa
    37381 actggtggaa cgttatctgg ctgagcaagc gatggatcgc gctaacagtt atgtgattgg
    37441 cgatcgcgcg accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga
    37501 ccgcgaaatc ctgaactggc caatgattgg cgagcagctc actaaacgag accgttacgc
    37561 tcacgtagtg cgcaacacca aagagacaca gattgacgtt caggtgtggc tggatcgcga
    37621 aggtggcagc aagattaaca ccggtgttgg cttctttgat cacatgctgg atcagatcgc
    37681 cacccacggt ggtttccgta tggaaatcaa cgtcaaaggc gacctctata tcgacgatca
    37741 ccacaccgtc gaagataccg gcctggcgct gggcgaagcg ttaaaaatgg ctcttggcga
    37801 caaacgcggt atttgccgct ttggttttgt gctaccgatg gacgaatgcc ttgcccgctg
    37861 cgcgctggat atctctggtc gcccgcacct ggaatacaaa gccgagttta cctaccagcg
    37921 cgtgggcgat ctcagcaccg agatgatcga gcacttcttc cgttcgctct catacaccat
    37981 gggcgtgacg ctacacctga aaaccaaagg taaaaacgat caccaccgtg tagagagcct
    38041 gttcaaagcc tttggtcgca ccctgcgcca agccatccgc gtggaaggcg acaccctgcc
    38101 ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg ataccggctg cgccaatctg
    38161 aactcggtga agtcagccat tgcgcgccac ggttatgaac caaaagtcag ccgtgacccg
    38221 gacgtggtgt tgctggccga taaactattt ttacccggcg ttggcactgc gcaagcagcg
    38281 atggatcagg tgcgtgagcg cgagctgttt gatctcatca aagcctgtac ccaaccggta
    38341 ctgggcatct gcttagggat gcaactgctg ggtcggcgca gcgaagagag caacggcgtc
    38401 gacttgctgg gcatcattga cgaagacgtg ccgaaaatga ccgactttgg tctgccgctg
    38461 ccgcatatgg gctggaatcg cgtttacccg caggcaggca accgcctgtt tcaggggatt
    38521 gaagacggcg cgtactttta ctttgttcac agttacgcaa tgccggtcaa tccgtggacc
    38581 attgcccaat gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac
    38641 ggcgtgcagt tccacccgga gcgttctggt gccgctggcg ctaagttgct gaaaaacttc
    38701 ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc
    38761 atcagggcga ttacggcaaa caacgcgatt acggtaacga cccgctgccg cgcttacagg
    38821 attacgccgc gcagggtgcc gaagtgctgc atctggtgga tctgaccggg gcaaaagatc
    38881 cggcaaaacg tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac gttccggtgc
    38941 aggttggcgg cggtgtgcgt agcgaagaag acgtggcggc gttactggaa gcgggcgttg
    39001 cgcgcgtggt ggttggctcc accgcggtga aatcaccaga aagggtgaaa ggctggtttg
    39061 aacgtttcgg tgccgatgcc ttagtgctgg cgctggatgt ccgtattgac gagcaaggca
    39121 acaagcaggt ggcggtcagc ggctggcaag agaactcggg cgtttcactg gaacaactgg
    39181 tggaaaccta tctgcctgtc ggcctgaaac atgtgctgtg taccgatatc tcgcgcgacg
    39241 gcacgctggc aggctctaac gtctctttat atgaagaagt gtgcgccaga tacccgcagg
    39301 tggcatttca gtcctccggc ggtattggcg acattaacga tgttgctgcc ctgcgaggca
    39361 ctggcgtgcg cggcgtaata gttggtcgtg cattactgga aggtaaattc accgtgaagg
    39421 aggccatcgc atgctggcaa aacgcataat cccatgtctc gacgttcgtg atggtcaggt
    39481 ggtgaaaggc gtacagtttc gcaaccatga aattattggc gatattgtac cgctggcaaa
    39541 acgctacgcc gaagaaggtg cagacgaact ggtgttctac gatatcaccg cttccagcga
    39601 tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt
    39661 ctgcgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc
    39721 ggataaaatt tccatcaact cccctgcgct ggcagaccca accttaatta ctcgcctggc
    39781 ggatcgcttt ggcgtgcaat gcattgtggt cggcattgat acctggtacg acggcgaaac
    39841 cggtaaatat catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg
    39901 ggaaacactc gactgggtac aggaagtgca aaaacgtgga gccggagaaa tcgtcctcaa
    39961 catgatgaat caggacggcg tgcgtaacgg ttacgatctc gaacaactga aaaaagtgcg
    40021 tgaagtttgc cacgtcccgc taattgcctc cggtggcgcg ggcaccatgg aacacttcct
    40081 cgaagccttc cgcgatgccg acgttgacgg cgcgctggct gcttccgtat tccacaaaca
    40141 aataatcaat attggtgaat taaaagcgta cctggcaaca cagggcgtgg agatcaggat
    40201 atgttaacag aacaacaacg ccgcgaactg gactgggaaa aaaccgacgg actaatgccg
    40261 gtaattgtgc aacacgcggt atccggcgaa gtgttaatgc tgggctatat gaacccggaa
    40321 gccttagaca aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga
    40381 ctgtggacca aaggcgaaac gtcgggcaat ttcctcaacg tagtaaatat tgcgccggac
    40441 tgcgacaacg acacgctact ggtactggcg aatcctatcg gaccaacctg ccacaaaggc
    40501 accagcagct gcttcggcga caccgctcac caatggctgt tcctgtatca actggaacaa
    40561 ctgctcgccg aacgcaaatc tgccgacccg gaaacctcct ataccgccaa actgtatgcc
    40621 agcggcacca aacgcattgc gcagaaagtg ggcgaagaag gcgtagaaac cgcactggca
    40681 gcgacggtac atgaccgctt tgagctgacc aacgaggcat cggacttgat gtatcacctg
    40741 ctggtgctgt tgcaggatca ggatctggat ttaacgacgg taattgagaa cctgcgtaaa
    40801 cggcatcagt gagttgcagg gtaagcggat gcgatgttgt tgtcgcatcc ggcaaaaaaa
    40861 aagggcaagg tgtcaccacc ctgcccgttt tctttaaagc tgcaaagatt acttcgcgtt
    40921 ataattacgc agagcgttac gccccagcac aatccccgcg ccaaccatgc cgcccagcag
    40981 caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag gtaacgtcgg
    41041 tttcatcaca taacggtaag catgaatatc aagatcatca acctttaagt tttcaatatc
    41101 caacaggttt tgacgtgtct gatagtagtt tggtgagaac accaacggac gggtcgcctc
    41161 atgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacaacgtat cttgtgtcac
    41221 atcttcagtc tgctgaacct gtggctttgc cacctgcgcc tgattcgcat actgcaacgc
    41281 ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcgactactt cctgcgttct
    41341 taaagagtcc tgcaagtttt tccgtcccag agcaatgttg tctttgagat ccttttctag
    41401 ctcttgattc actttatcat caacttgctg aatgtattgg gccaacttca tttgtgctcc
    41461 ctctgcagtt tgcccaacat aagaaacagt caatggtaat tgctggttct taacagaagg
    41521 ttcgatggta agtttttctg gttcttcctg attatccagc gtttctgcta atgcagagaa
    41581 ggcagaactg aagcgaccaa ttaacgtctc ctgtaaatcc gatactttcg gtgcagcctg
    41641 accatagata acattcatgg cattgttata gccagcaatt tgccccacgt cgggctgagt
    41701 gataattgct gttgacgtcc atttctcctt cgctactgcc aaatatccaa tagccagggc
    41761 aatagccaca atgacggaaa tgatgattgt catcttgcca cgccacaact gcactagtaa
    41821 atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat tattttctac
    41881 tctcatcatt accctaactg acggaaattt ccaagtccgc atagtttagc gataatccta
    41941 gtgaatgcta taggataagt gataaaaata atgaattatt ggggaggtaa caaaagacaa
    42001 tcagggcgta aatagccctg ataacaagat gttagtcgct gccaaagaga tcgcgggtgt
    42061 ataccttatc tgccacatct ttaagctctt ctgccattcg gttagagata atgacgtcgg
    42121 cttgttgttt gaagatggcg agatcacgtt ccagccgaga gttgaagaat gagtcttctt
    42181 tcatcactgg ctcgtagatg atcacttcaa cgcctttcgc cttgatacgc ttcataatcc
    42241 cctgaataga agacgcacgg aagttatctg aaccgctctt cataatcaga cgataaatac
    42301 ccaccacttg cggtttgcgt gacaaaatgg catcggcaat aaaatcctta cgcgtgcggt
    42361 ttgcatcgac aattgccgag atcaggttat tcggcacaga ctgatagttc gccagtaact
    42421 gcttggtatc tttcggcaag caataaccac cataaccaaa cgacgggttg ttgtaatggt
    42481 tgccaatacg cggatcgaga caaacgcctt cgataatctg gcgagtattc agacctaaac
    42541 tttctgcgta gctatccagt tcgttaaagt atgccacgcg catcgctagg taggtgtttg
    42601 caaaaagttt aatcgcttct gcttcggtcg aatcggtgaa cagggtcggg atattttgct
    42661 taatcgcgcc ttcctgtaac aacgcagcaa aacgttctgc gcgttctgaa cgctcaccga
    42721 tgacaatacg tgaaggatgg agattatcgt aaagggcttt accttcacgg agaaattccg
    42781 gagagaaaat aatattttca gtacgatatt tcttatgcat cgcttcggta aaaccaacgg
    42841 gaaccgttga tttgatgacc ataaccgcat aaggatttat ctcaactacg tctttaatta
    42901 ctgattctac actggatgta ttgaaataat tagttttagg atcatagtcg gttggagtgg
    42961 cgatgatgac ataatcagca tcccggtagg cttcattttt atctaatgtg gcattaaagt
    43021 gtattttatc tgattgcaaa aactgctgaa tttccttatc aacaatagga gatatccgat
    43081 cattcagcat agcaacgcgt gacggtaaaa tatctaatgc cacaacctca tgattttgtg
    43141 cgattagaag cccgtttgac aagcctacat agccagtacc ggaaatggtg attttcattt
    43201 catgctctca gaattaactt aactgtgaat catgatgttt ttagcatcct gataagagct
    43261 aaatgtttta acgctacgtt attagctggt caacgctacg aaaaacaatt cagattttct
    43321 ttataaggat agctgatatt tattaagtta atattaagca gtgaaattta gtctataaga
    43381 taaatggcaa aaaaaaagcc cggtgcaata tacgccgggc ctcaatttta ctgttggtta
    43441 aatcagatta atccagccat tcagtatgga acacaccttc tttatcaatg cgcttataag
    43501 tatgtgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga acagcggcac
    43561 ggtagctatc gtaataggca accgcagcgg cgaaggtcgg aaccgggata ccgttctgta
    43621 ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt
    43681 acggagccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg gtgattttct
    43741 gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt tcgccgtagt
    43801 tcagatccca gttgtattct tcagacgcag cacgcagctg agagaagccc tgagcgtaag
    43861 aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca gccttgtcgc
    43921 ctgctgactg agcttgcgga ccagagagaa ctttagatgc ggcaacgcgc tgatctttca
    43981 gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt tctccgagat
    44041 ccagcgcgct ctggctggtc catttaccgg tacctttgtt agccgcttca tccaggatca
    44101 catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatatctttg gtgatgtcga
    44161 tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgtgcc agttcttcgt
    44221 tagagagatt caggccgcct ttcaggagag aataggcttc agcaatcagt tgcatatcac
    44281 cgtattcaat accgttgtga accatcttca cataatggcc tgcgccatca gcaccgatat
    44341 aggtaacgca cggttcgcca tcttcagcaa cggcggcgat tttggtcagg atcggcgcaa
    44401 ccagttcata ggcttctttc tggccaccag gcatgatgga cggccctttc agcgccccct
    44461 cttctccgcc ggaaacaccg gtaccgatga agttaaagcc ttcggcagaa agctcacggt
    44521 tacgacgaat ggtgtcctgg aagaaggtgt taccaccatc gatgatgatg tcgcctttat
    44581 cgagatatgg tttgagcgaa tcaatagcag catccgtgcc tgcacctgct ttcaccatta
    44641 acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta tagtaaggaa
    44701 ccagtttctt gcctggattt tcggcaatca cttcttccgt cttttcacgg gaacggttga
    44761 aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc cccatcactg
    44821 ccataccgac tacgccgatc tgttgctttg acattgttta ctcctgtcag ggggtggtca
    44881 ccgccgagtg tgcggcttaa atgtggtata gatgttaact cactcataga gatggtgaat
    44941 agcattacct ttgctatacc acactgaata tctcaccttt tccgtctttt aaaacttatt
    45001 tatatgaaat tactgaatta cttgttcagt aactcaagaa taagttttgt cttttcttcc
    45061 atcagcggca catcaccgcg agattccaca ttcaaccgca ccaccggttc ggtgttggag
    45121 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcggt gcgatccacc
    45181 gccagcgcct cgcggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    45241 agtttgctgt tgatctcacc gcttgccgga aacgctgcca tccggtcgcg caccagttcg
    45301 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    45361 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcacc accgtagatg
    45421 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga catcaccggc
    45481 gtgccgcctg cggcagtcac cacatcaacg gtgttccagg agagacgcgg atcgtggatg
    45541 atcttcgcgc cgggattttt ttcgaggaat gcttctgcca gcaggccgac aatgtagtag
    45601 ccctcaataa actgcccttt ttcgtcaaac aggaagcagc ggtcaaaatc gccatcaaaa
    45661 gcaatgccca tatccgcacc gtgtttgatg accgcattgc gggtgtcgtc gcggcattcc
    45721 ggcagtagcg ggttaggaat accgttgggg aaattgccgt ccggcgtgtt gtgcactttg
    45781 attaattcca cgggtgcgcc aagggcttta aagcgggctt cgatggcgtc caccaccgga
    45841 cccgctgcgc cattcccgga gttaatcacc agcttgagcg gcgtgaggtt tttgacattg
    45901 atataaccga acaggtgatc aacgtaagcg tcacgcagat tgatttgctg atagcgaccg
    45961 cgtttggttt catcaacggg aggaaagtcg ttggcttctg ccagacgctg gacatcgcgc
    46021 agtccggtat caccgctgat cgggcgagcc ccttcgcgca ccagtttcat gccgttgtaa
    46081 tccatcgggt tatggctggc ggtaacttca atgccgccat ccacgccgag atggaacgtg
    46141 gcgaaataga tctcttcggt gccggacatg ccaatatcca gcacatcgac gcccgcatcc
    46201 tgtaaacctc tcgccagcgc cagttttaag gtttcgctgg tgaggcggac atcaccgcct
    46261 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    46321 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    46381 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    46441 gtttttgtcg ttatttacat ctcggtttcg cattcattgc ctgatgcgac gtttacaccc
    46501 gtccgtagcg atccgcgaaa cgcaccacat catcctcttc gagataagag ccggagcgca
    46561 cttcaattaa atcgagcgga attttccccg ggttttccag gcaatgcatc gcccccagcg
    46621 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    46681 ccgttcccgc gacaaccacc cagtgttccg cgcggtgatg gtgcatctgt accgacaagc
    46741 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    46801 catatttgcc ccacgggcga tagacttcgc gatgcacccg atgctcatgg cgaccatcgg
    46861 ctttgatctg ctcgaccact tttttcacat cctgcaccgc gttacggtcg gcaatcagca
    46921 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtt acgaggccag
    46981 attcggcgta cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    47041 tgccctcggc ggtgtgggcg ctaatttccc ataatgagga ccaggagccg acatcgctcc
    47101 agcccgcatc catcggcacc acaacggcat ctgccgtacg ttccatgacc gcgtaatcca
    47161 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    47221 gatcgacggc gctcatcgct ttttcacagg catcgagaat atccggacga tattttttca
    47281 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    47341 cgcttgccac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    47401 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    47461 cggtttctgg tagatccggc acaatgccga aggtcaccag cttgcccgct tcggcatatg
    47521 gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaatcaca tgatccgccg
    47581 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    47641 gcgcaatggc aggtgccgtg tttcgccctg ccggttcgag aataatgttc tcggtaagtt
    47701 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    47761 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    47821 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataagcggc
    47881 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    47941 aatatcattt ataaattgac gtaacacgtt ctctttatcg agtgtgcgtt cggcatattc
    48001 acgtgccacc gtgttgtgtt tgggcagcag gagcgcctga cggatccccg ccaccagcgc
    48061 ctcgaccgat tccggttcta cgcaaaccgc aatgcccgga aaggtttcgc aaagctgccc
    48121 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    48181 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    48241 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    48301 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    48361 aatggcaaaa atcagcggtt catcgcgcag acgatcggca gcttcaataa cgttttccag
    48421 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggtc
    48481 aagctggtta cgaagggcgt caacatcggc atctgcaaca tgctgaaaac gggcaatttc
    48541 cgaccagttg gggaagaaga tgacgttttc cgccgccacg cctttttcga tggctttatt
    48601 catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc tacgttcgaa
    48661 cgccgttgcc agctgtgcca ctttgctgcc tttgcctttt ccggcaaggc ccagccccag
    48721 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag aaagtttcgc
    48781 cagcaggcgc attcccggcg tgcaaaacag cgttggcacc acgccgataa tgcgatccgg
    48841 cttccagcga cgttgcgcca tcagaggaaa gaaactgctg acggcaaaac tgccgagatg
    48901 taacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    48961 ggcggccccc tcttctcgtt tgtaccgcca ggcggaatag ttctcgccca cctgccactg
    49021 cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgtgccg ccagccattc
    49081 caccatctcg ccggtgtatt tgccgatacc ggttaactcc ggcgagtagt taatgccgta
    49141 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    49201 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    49261 tcatgctgct catccggcag tatcagctct tcttcagcga ctctgaagcg aaaaccgagc
    49321 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa gtgctgccag
    49381 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    49441 cgctcgaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    49501 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcaccgcg actgttctcg
    49561 acaataaagt cgagagagac aagcggggtg gagcgcacta ccgtggcaaa gtcttcctga
    49621 cgtaaaaaca tcattacccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    49681 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    49741 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    49801 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    49861 tgccgacgtt aatgtgcgac agcatcggct gggtgttctc cagccagact tcgtgcgcca
    49921 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    49981 gtgtaccgct gccccatacc accacgtccg gtgcattctg tgccgtcgcc tcgtggaagc
    50041 gacgcagcaa tgcagggatc acatgcgaat tactcgggtg gaagttgtca tgcgggccat
    50101 acaggttggt cggcatgact gagcggtaat cgcgtccgta ctggcggttg tatgattcgc
    50161 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    50221 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atacaggacg
    50281 atccaagaaa cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    50341 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    50401 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    50461 ccgcgcggct gtccagcagg ttcagctcgt cgcgggtgcg taataccagt tccacatcac
    50521 cgcgctgttc gagctgccgc ctgatggcag aaccgaccat cccacgatgg ccagcgataa
    50581 aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    50641 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    50701 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    50761 gagcagcgtt tcaacttcag ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    50821 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    50881 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gccgctgcca tttccacgaa
    50941 ctgacgcacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    51001 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    51061 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    51121 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    51181 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    51241 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    51301 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    51361 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    51421 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    51481 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    51541 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    51601 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    51661 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    51721 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    51781 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    51841 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    51901 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    51961 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    52021 ttatttcacc gagggtatat aaattgacgt catcgccgac ccacgcgtaa tcacctaagg
    52081 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    52141 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    52201 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    52261 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    52321 tgttatataa ggctttcgtc tttgaggtag tacgttggcg taaatgccag gataattcag
    52381 cccaaaagcc aggcacatgt aatatttgtc gttggacttt tttcgcgtca gcacacaatt
    52441 ccatattatt ggtggtagat accccaccca tggaaaattc agacaccagg ccattgagtt
    52501 ttttaaatgc ataaccggct ttatacattt tggctgccag cgcgtagtcg gaagaaactt
    52561 tatattccag gtcataacac cattttttca agccggatac agggaaaaat atcgcctgat
    52621 gactggcggg caggctgtga taaatatacc agcccggttt ggcgctacgt ttaattttat
    52681 gcccgtcgcc aaaatccagc agcgcatcgc cggtgatcat catgttatct ttttgcattt
    52741 ttaacttacg gacaaaattt gcggcatcct gatgaaaaat atcgcccgaa ttgagaaaca
    52801 acgcgaactt gccttgtgcc atcgtaatgc ctttgttcat ggcgtcgtag ataccgttat
    52861 ctggctcgct gacaaagcgt aggttataga taccattgag attttccaga tactcacggg
    52921 tgccgtcatt ggaaccgcca tcgacaacaa tccattcgaa gctgatatct tccgcctgcg
    52981 ccagatgcgc cagcgaggca tgtgttttga ctatcccttc gaggttacga aacgcgacag
    53041 tgattatgct aagcaacatg ttcttattct tacctcgtaa tatttaacgc ttttcgcaaa
    53101 ataaatggac agacgattaa aaatgcatat tccgggctaa atatcgaacc ggtaaaaaac
    53161 agcgataccg gagtaaaaag atataattgc acgcgaaaat tgcggttatc accaaaagcg
    53221 ttgatcatca tttttatcac tttccccatg taccacagcg ataaaaacac tgcgaaccag
    53281 gaaaaataaa taatcagcag atacaaacca ttgtctatgg tttttccgac atccgcaccg
    53341 ttaaatattc cgaatgatgc gacgtattca taaagtgagc caaatctgac tacaccgtca
    53401 atatgggtca aagaataacc gaccatcacc agcggaccga caatacgata atacgatgac
    53461 gatccttccg tacctaaatc gcccagacgg gtggaaatat aaggaaacgc gattaccaca
    53521 ccaaccagga atacagccag agaaatcaat gccaacggta actttttctt aatcgcctct
    53581 ttattcagat attgaaatgc ccactccagc aaataaaaca ggataaaagt cataacccct
    53641 gaaaacgatc cggataatat tatccctgcg agaatcatag catcggtttt aggcgttttg
    53701 ataccaaact gtttgatgct gagccaaatt gagattaatg ccagagcgaa aaatgccggt
    53761 tcgaaataaa gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag tacatagctg
    53821 ttgctgtaaa tcagatattt cgaaatcgac tccataatac tgctgccacc ggtgaggata
    53881 atttgcgcca tctccaccgc cgccagcgcc accaccagcc cgatcaccag ataaaagaaa
    53941 cgtaatatct tgcgatggtt gtgtggcgaa atcgttttaa agcgaatact ccacaccatg
    54001 ccaataatga tcacaatata gacaaacagc atggttgaag tgacgtattt actggcatcc
    54061 agcgactgac caaacagata gttaaacgcc gtgagtcccg caccaatccc taaggcaatc
    54121 atcaattttt taacgctgat gcgttctaaa aacagcagta acaagacagg taaaaaagtg
    54181 acgatggtga tggggaaact ttcgccaagc tgggcgattt tgacgttaac cagcaggtag
    54241 atcagcggca gcagcaggta gctacagatt ctgatagaag ttgacatact cctccagcat
    54301 ctgttgtcca ctgtaggtgg cgcggctgcg ttggctgaac tcagccagcg tggtaccaaa
    54361 tatcgcctgc gcgatttccg gtttgcttaa ctgcaccagt tgcagcacgt cttcttcgtc
    54421 gacggtttta ccgccggatt tttgcaacac ttcccgtgcc gcgtcgctat gggtggcaat
    54481 caccggcacg ccaattgata acgcctcaca caaaatcagc gggtagttat cgacgcgaga
    54541 actgaatacc agcgcatcca tctgattgag cgcgctcatc aacttgcgct tgtcagtttc
    54601 aaagccgtga ttaaccacgt tgccagcggt gaacggcgag aacttaccaa aggtatgcag
    54661 ttcgattttg tcgcccagcg ccatcatctc acgcaccagt tgctggttag tcttgccgtc
    54721 gtaacgcaag tcatgcgcca ccaccgcgat tttcggcttg ccctgggttt cgcgcaccgg
    54781 aggcaagtcc gccagaatcg cttcggttgc catatcaata ccattattga taatccggca
    54841 acgccctgga ccgtacaggc tattgaaagc gtcagccaca tgctggctgg gggaaataaa
    54901 ctgacagccc agcgccagca tctcacggaa taactggcgt ttgcccgcca ccagctggtg
    54961 tgcgcgatca atcttcaccg gcggatagtt atttaaggtc gggcatttct ggcagcccgt
    55021 tttccagcct tcgcaaccgt cggtaaaggc gcagcgtccg gtaacactcc agtggtcgtg
    55081 cagcgtccag accagagtga cgtccggttt atggtttttc actttttcgc aaaagcgcac
    55141 cacgctctta agatttagcc agtagctgtg cagcacatga aaatgcagga ccaccggacc
    55201 cggtgtgcga gtaatggtgc gatataactc attgaaattg ccaaacagat cgcgattaaa
    55261 cagacgaaac agggcaatat tcgccatcgc ggtcatccgc ggcgtatgtt tgatgacctg
    55321 cggatagttc tgatggctga cgctctcttt gccgcctttg ccgtaaccgt agacaaaatg
    55381 tgacgccagc ccctgttgca gcgcacgctg gtggagatct aacgccacac ctgctgcccc
    55441 gccttccgcc agtcgcacat taaattgcag aatattcatt taattacctt cactcgcgct
    55501 ttttctccca ccaccagcgc gttgtccggg acagagtcga gcaccacgct gcccgcaccc
    55561 acggtgacgt tgttaccaag cgtgatatca ccaagaataa tgacgttggc accgagttcg
    55621 acgccgttgc caatgtgtgg acatgccatg ttatcggcac cacgattgcc gatagtgacg
    55681 ccgtggcgaa tggtgaaatc atcccccgct accacgtttt tattgatcac tacggcgtaa
    55741 ccgtgatgga tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc ataaccgaaa
    55801 aagcattcgg tgataatgcg atacagcacc agcagcgggg ccgcccacag attgttgagg
    55861 acgttctttt tgcgccacac cgaacaaaaa tgagcaacac gataggcaag aaccatgcag
    55921 cacgggcgta aactccagct gttggcacgc agatcttcca gcatctttag cgcccccgaa
    55981 taccatcagc cagacgtttg ccgttacgca ccgacagcag cgtcagcaaa gtgcgccagg
    56041 tcatacgttt attgcggatc tggtagaggg taaacagctg atattttttg cttgcacggt
    56101 caaatttatc tttgtgcttt cggtaaaagt ggaagtaccc ggagaatttt ttcggtgacg
    56161 aggtgatctg catttcaccg tgattgatat gcaggatctg cgtcgcttct tccactttcc
    56221 acggttcgcc gtactccacc accatccgca ggaagatgtc gtaatcctgt gctgctttca
    56281 gttcggtatc gaacaaacac tctttgaaac gccatgccca agtaaagacc tgattgccaa
    56341 tgatattgcg tttatagaac aggcggcgtg agtacggcga tttgggatac agtggtaagc
    56401 ttgccggttg ggaataaact tctccctggc atacgtagtc gttagcgtac aaaaaggcgt
    56461 gagtgaccag ttgctgttta tgggcgagga agacgctcag acggttgggt gtccattcgt
    56521 catcgtcatc gatcccggta atgtattccc cttgcgccag cataatcgcc tggttacgta
    56581 ccgcgcacgc cccgctgtta atgtcgttgt gaatgtaggt gatacgcgga tcgttgaggg
    56641 cagtgacgta ctgttgcagt tgttcccagg aggtggagca atcatccacg atgatcatct
    56701 cccagttgct gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc agttgttggc
    56761 ggttccaggt cggcatatag attgaaatca gcggattctc tttcataggt tgttctcccg
    56821 gcttatgagc tggtttgctt gccggatgcg gcgtgagcgc cttatccggc ctacggggcg
    56881 gtgcgaatgc aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt aatagccata
    56941 atcctgatac gcgctggcgc ggcggaagat ggagttcaga atcacccctt tcaccggaat
    57001 accgttttgc tcaaagcggc tcagactggt ttccacttct ttcaatgtgt tgaccgcata
    57061 gcgcgccacc attaacgtgg ttccagcgtg acgcccgaca attgctgcat cggtcactgc
    57121 cagaatcggc ggcgtatcaa tcaacaccag gtcgtagttt ttactcgccc agctcaccaa
    57181 ttcggcaaag cgttcgctca tcaacagttc agaaggattt ggcggcacct gaccgcgcgg
    57241 gatcaggtca aatttggcaa tagaggtcgg tttagctgcg gtagtaatat cgccctggcc
    57301 aatcagaatt tccgacaggc cattgacgtt attggtaccc aacagctcgt gggtgtagcc
    57361 tttgcgcata tcgcagtcga tcaacaacac ccgtttattg gtctgactga tcaccgccgc
    57421 caggttggcg cagacaaagg ttttaccgat tgacgggctc acccctgtca tcatcaacac
    57481 attgttctgc gcctgcatca tcgcgaagtg cagactggta cgcagactgc ggatggcttc
    57541 aatcgccaga tcggttggat tccccaccgc cagtagctgg ctctgtttat agcgtttaat
    57601 ccctttgatg gttttgacgc tatcgcgcgc tttctgccat tccgacagcg ggatgctggc
    57661 atagacgctg ataccatgtt cttccagcac ctgcgggctt tcgatgccgc gattaaacaa
    57721 cgagcgcagc agcacaccca cgatagagag catcaggccg aggataattg cgccgaggat
    57781 aatcagtcct ttcttcggtt tcagcacgcc aggctgggtg attgccgggt caacaatgcg
    57841 cacatcgccg acggtgctgg cttcggtgat tttcagctcc tgctctttat tcagcagttg
    57901 catatagacc tgctgaccag actcgacatc gcgggtcagg cgcacaatct cctgctgggt
    57961 tttcggcatc gccgttacgc gaccgttaag tttggctttc tcttcttcca gcgcctgacg
    58021 tttctccagc agcgtgcggt acgccgggtg aactttggtg tacagcttgg agatttccgc
    58081 ctctttaaag gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat cgagcaccgc
    58141 tttcgcttcc agcggcagat caacagaatc tttatcctga cggaaggcat tcagtttgtt
    58201 ttcggcaaca tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga aagcgaggct
    58261 tttcgacgct tccgccgatt tgcgctcaat attttgttcc tgatagttac gggcgatgct
    58321 gttaagaatg tcgcggatct gttcgcgatc ttcaccggta taagtcaggc tcagtacgcc
    58381 tgcgtctttg ccgttctccg ttaccgtcag gctgttttgc agttggttga tcatccccaa
    58441 cgtggagtat ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg cttcaaccat
    58501 cagcgtgacg ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc taaagccgcc
    58561 atcactgctc agggtgtagt ttttattgtc cagcacatta agcgtaaaca cctggcccgc
    58621 catctctttc gggcggttaa aggtagtcac tttcaccgtc tcgttctggc gtcccatcag
    58681 tcgctcccag cccgccccga aaatcgggaa cgtgtttttg ctcactgcaa tatcgaggtc
    58741 gagatcgtcc accgttttac caagcaccag acgcgaacga atcaactgga tctcggcgtc
    58801 cgatgcaggc ggtttgttgg ctaatgccga accaatgtcc tgcactaacg aattgccgct
    58861 gttttgctcg atttgtacca gtgcgtcggc actataaatc ggcgttgcga agaaggtgta
    58921 aaccacggca cagagggcaa atacagcggt gatgccaatc acccaccagc gcgcttcaat
    58981 gacggtgccg accaggcgac caatatcgat ttcatcactg cccgttaccg gagcggcatg
    59041 ttgttttact ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg cgcccactgg
    59101 cgggcagacc gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct tttgcgatac
    59161 ggatcgggga tttcacattc gttatcccag tgaccaaaca gcatcacttt gccgcgcatc
    59221 tcaggtgcca tctcgcataa gcgttcgata tggcgctttt ccatggtcaa aatcaggtca
    59281 tagttgcgac acaggcggcg gctgatttgt cgtgcacagt ggccttccag agacagttga
    59341 tgttctgcgg cgacgctgat agcggtagga tcagcgccct taccgaccag cgcgccgagt
    59401 ccagcggact ccactttcag ttccgggtga taacgttgca gtaagcgttc cgccgtcggg
    59461 gaacggcaaa tattgccgac acagacaact aatatgttgt taaacatgac gattaccagt
    59521 tatgaatgtc gctggctgta tccgtcatgt aacggacacc gctaatagtt ggcaacaact
    59581 gattgatcag acggttccag cgggaaaccg gggcggtggt gacatacacc acgtcataag
    59641 gttgcaggcg gaattctgtc gccatcacca gcgacgtggc atcggacata tccagctggt
    59701 agatattggc aatcttgcca ttacgcccgc cctcgccttt cagcggacga atgacaaaga
    59761 tgccgctggc gttggaggtg gtcatgtcga taccttcagc attacccagg gcttcagtca
    59821 gggtcatgcc gctaaagtcc attttcaggg tgctctgttt cttcacttca cccatcacaa
    59881 atactttcag atcatcatta cgcggcacgt agagaatatc gccgggataa agcagtcggt
    59941 tctgattgag gtcgccgttt tgcatcagcg cctgcaaaga aatgcgttct tcacgaccat
    60001 tgtgtgttag cactacgttg cgccagtcag cggtgtcggt caggccacct gcggcgttga
    60061 tggcgtcgag aatagtcagt ggcacgttgg tgatcgcctg ttgaccggat ttattcacct
    60121 gaccggagat ataggccttt tgtgagcgga aggcggcgat attaacgtcc acctgcgggt
    60181 cagcgatgta cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg agcgttttcc
    60241 cgactacgtg aaccttgccg atatacgggt aaaacatagt gccgtcaggc tgtacccagt
    60301 tgccggtgtc gctggagctg cggtactgac cggctggcgt ggtgagttcc gggtgatccc
    60361 agacggtgac attcagaacg tcgcccggcc cgacgcgata ctggtaattc gcgatctcgc
    60421 tttccagcgt catattgggg cgcgctacat tcgggcgtgg gcgtaattgg tcaatcaggc
    60481 gcggggtcag cggataaaca ttcaccattt tgtcgagatc gaaatcagcg tcctgctgtt
    60541 tgatgacgtc tttgcccatc gtcgacatat tgctgcccgg aagtactgtg caaccgctta
    60601 tcaaggttac tgacaccaat aatggcatca atttcatttt ggatttcatc attgtttatt
    60661 tatcactttg gcagagtaat tatcctgtgc actattaata gcaatatcgc catgcacatt
    60721 taccttgcag ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa attgatttca
    60781 tccgcaggct attgacagaa taattcagac tggtctttca ggcatccaga cacgctaccg
    60841 cccctggctt tttagccacc aatacactga tttagcttaa tttttcaaac cctctctgca
    60901 tacaatcgtt gatgagaaag ggttattgcg gaaattaact tccgaatata aggtgacatt
    60961 atggtaattg aatattggct ctccaataat gcaggaggaa gtgttacagc taacggaata
    61021 gcaggcaagg taacaattcg gcaattggct atttttaaga attatattaa gtgtcattca
    61081 atatggtttt taggagtttc tttaggttga caatatttaa tattgcgcct ccacatgcga
    61141 tatttcttaa ataatgtttt attattacca cttttaattc agggataatg tgaggttatt
    61201 accctcaaat aatatgagat aaatagtgct gcaacattgc atttttgccc cgatttatcc
    61261 atgatcgaat tgtgacattt gtcatacaac gaataggttt tgtacttact atggaatgga
    61321 ttgccgatcc gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa ctggtcctcg
    61381 gcattgataa cctggtcttt attgccatcc tcgccgaaaa actaccgccg aggcagcgcg
    61441 accgcgcacg ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta ctgctggcgt
    61501 caatctcctg gctggtcacc ctgactcaac cgctgttcag cttccgctcg tttaccttta
    61561 gcgcccgcga cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa gccacgatgg
    61621 agctgaacga acggctggaa gggaaagaca gcaataatcc cacacaacgc aaaggtgcga
    61681 agttctgggg cgtggtgacg caaattgtgg tactggacgc cattttctca ctcgactcag
    61741 tgattaccgc cgtcgggatg gtcgaccatt tactggtcat gatggccgcc gtggttatcg
    61801 ctatcagcct gatgttgatg gccagcaagc cgttaacgca atttgttaac agtcacccga
    61861 cgatcgttat tctctgctta agcttcctgt tgatgattgg ctttagcctg gtggcagaag
    61921 gtttcggctt cgtcattccg aaaggctacc tgtacgctgc cattggtttc tcggtgatga
    61981 tcgaggcgct caatcagttg gctatcttta accggcgacg ttttctttcc gctaaccaga
    62041 cgctgcgcca gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa aaagaagatg
    62101 cggaactgga cgccgaaacc gcgtccatgc tggtggatca tggtaaccag cagatcttta
    62161 atccgcagga acggcggatg attgagcggg tacttaatct taaccagcgt accgtcagca
    62221 gcattatgac gtcgcgccac gatattgagc atatcgatct caacgcgccg gaagaagaaa
    62281 tccgccagtt gctggagcga aatcagcata cgcggctggt ggttaccgac ggtgatgacg
    62341 cagaagattt gctcggtgtt gttcacgtta tcgacctgct acaacagtct ctgcgcggcg
    62401 aaccgctcaa cctgcgggtg ttgattcgcc agccgctggt gttcccggaa accttgccgt
    62461 tgttacctgc cctggagcag ttccgtaatg cccgcacgca ctttgctttt gtggtggatg
    62521 agtttggctc ggtggaaggg attgtgacat taagtgacgt cactgaaacc attgtcggta
    62581 acttaccgaa cgaagtggaa gagatcgacg cccgccatga tattcagaag aatgcggacg
    62641 gttcctggac ggcgaatggt catatgccgc tggaagatct ggtgcaatat gtgccgctgc
    62701 cgctggatga aaaacgtgaa tatcacacca ttgccgggct gttgatggaa tatttacagc
    62761 gtattccaaa acctggcgaa gaagttcagg tgggggatta tttgcttaaa acgttgcagg
    62821 tggaaagcca tcgcgtgcag aaggtgcaga ttattccgct gcataaggat ggcgagttgg
    62881 agtacgaggt gtgatggtat cggtcatttt acccaatagc cgaaaggaat aagatgaggg
    62941 aaccgcgcca gcggcgattt accgccggga gcccgggttg ccagggtggt ggcgattgag
    63001 ccaccctggc acgttcaccg gcttgagcgt tccatagtag cgaggaacat caagtgaacg
    63061 gaacagcctt cgatcttaca tattcagaat ataatgatgc cgccacgaac gcctgatgcg
    63121 acgctttacg cgtcttatca ggcctacagg actggtcacc tggtaaccta catcttctcc
    63181 agcaacttct tcacatcctt gccattgcgg gaatctttat tccgctcggc ccagtcattc
    63241 aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac ttgcaggctg
    63301 taattgagtt gctgccagtt gccataaacc cgcagcggta ctggcgtttc tttcaggaaa
    63361 tcaatcagtt tactttcccc gttccagcca cccacgaccc gaatatcaaa ctgggtatcg
    63421 caggtttgat ctgccagatt caacatgcct tcccctgtca gcgccagcac aggcgattga
    63481 ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt ggtaaagcgg
    63541 tcaagccgcg tcacgttatc aaagttttca gcggccttca catcaccgcc attacgctct
    63601 accgcttgct gaatcatctg ctggaagttc atcccttcca tgcgcgtgtc ggtcatttcg
    63661 acatgtgcct gtccttgcca gttgtggcgg aatgcgtcgg catctatgtc agcaccggag
    63721 aaatcaccag ccagtgacat ttttccggtc aacgaaatcg gatagttaaa cgccttcaga
    63781 atggtaccaa tctcaacgtt ttccagccgt ggctggaagt ttatccgcgg atttattgat
    63841 gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt taagtttgcc ctgcagttga
    63901 gtaatttcca gcaaacccga cttgttggtc atttgcgtgg caacatctgt aaaattcatt
    63961 ccgcgccagc gcacgttact ggcctgcaac aaaatatcag ccgtaaagcc ttgcagtccc
    64021 tgataggccg gttcatcaat acgcgaagaa atgaccgggc gcggcaacgt tgattggctc
    64081 tgcccctgct gcgcggcacc gttttcacca ttcgcggttt cattaagcgg gatgaggttg
    64141 tcaagattca gttgcgggaa ttgcagtctc agctgccatt ccggtttctc tgtcagcgtg
    64201 acctgtgctt gcccgctcag cgtactgtca ttggcggtca agctgatttg gttaaatgaa
    64261 aggcgtttat gtgactcctg ccactgggcc tggaaactcc cctgcccctg aataccttgt
    64321 tttggtaaat cggcaccctg caactgccag ttaatttgtt caatagccgc cgttaaatca
    64381 tgcggataat cagaagcatc caccgtaccg ttaagggata ttgtgagatc gcgctgatcg
    64441 cgattaaccc gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg ttccatttgc
    64501 aggcggatat tgcggattgt cacctgctcg tcatcttcat gctggaacac cagcacgctg
    64561 tccgccacct taagactgga tatatcaaac gaccatccgc gatcgtctga cagatccggc
    64621 aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc cgtctgcggc
    64681 gtcagttgga tcactgcccc ttttagcatc acctgcttaa cgctcagttg atgactcagt
    64741 agtggtaaaa gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg ctggcttgcg
    64801 ccctgggcgg tgagagacat tcgcccggag aggatactaa gctgcggcca gacgtgccaa
    64861 cgcagtggcc cgtcgagctg caattgataa ccgctacgtg cagcaacttg cttgaccata
    64921 tagtcgcgga aatcattcgg attcaccagc aacactaacg cagataaccc ggccaccagc
    64981 acgaccagga gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc aatgggcctg
    65041 aattagtctt tatcgattcg gctggctacc gcgccctgct ggttgcgata tttcgcatct
    65101 tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct cagcgcacca
    65161 attaacatgc ccggacgcag cgccagcggc agcttaccgg agttgtagaa ctccagcaca
    65221 atgcaaccag accagcccgg atcgatacgg tgcgcggtga cgtgcaccat cagccccaga
    65281 cgcgccaaag aggaacgccc gtccagccag cccaccagat cggctggcag cgtcaccgac
    65341 tccagcgtca ccgccagcgc cagctctcct gggtgaagat agaacgcctc gccctcgtcg
    65401 agaacgatct catcgctcat cacgcggtca agcgcggcgc tcacttcatc tttgggaccg
    65461 ctcagatcga taaacgctgc cgtgtgacca cggaaggtac gaaatttatt gcccaggcgt
    65521 acatccaccg tcgcgccgtt aatacgctcc actggcggac gtgggttgat cgacaaacgg
    65581 ccttcatcaa gccaggcttc aatatctcgg tcacacagac gcatggcatt ttctcctttc
    65641 gcgcatcact cccttaacgc caattacgtc aagggcatac caggttatca ctgaacggta
    65701 cacaatttat caagcttatt caaagaactg acttattttc gctttcaata tatcgatcgc
    65761 gatgcggttt ttcccgccgc gcggcacgat aatgtccgca tattgtttag aaggctcaat
    65821 gaattgcagg aacatcgggc gcacggtttt ttgatattgc gccatcacgg aatccattga
    65881 acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc aggcagatat ccagcggggt
    65941 atcaacgaaa atggagaagt taagttcgtc acgcaaacgc gcatccgtca gcaacaaaat
    66001 gccttcgaga atgatgacct tcttcggctc aaccgtcacc gtttctttca tacgcgtatg
    66061 ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg cttgtaaatg
    66121 ctcaagcagc aggctgtgat ccatcgcgct gggatggtcg tagttggtct taacgcgttc
    66181 ttccatcgac agatggcttt gatctttgta atagcagtct tcgggaatta cgccgatgtg
    66241 ttcatcaccg acttgctcac gtaattcacg ataaagggta ctggcaataa gactcttgcc
    66301 ggaagccgat gcgccagcga taccgataat gacgcactga tgagactgat cagtcatata
    66361 tttagcgacc tgattaacct ggatgttagg aagggggcga cgaagcgcca aacgcggcaa
    66421 ttatagggat ttcatccgtc tgataccagt cgaatagcgt tgccgcgcgc tcagagttaa
    66481 ttgttgacaa agaattcccg gtggcaaatt acgttgatca gttttatgta aggtaaaaaa
    66541 tgttatacgc agttgcgcaa attatccgcc tttacgtcac tttatgagca attcgcatat
    66601 aaaatgtaaa acttttgtac tagcataaac acagaaacga atactggcga cctggtcttg
    66661 cggataaagc ggtaatgagc aaacaatcac agcatgtatt aattgccctg ccccacccgc
    66721 tgcttcacct ggtcagttta ggtttagtct cgtttatctt tacccttttc tcgcttgagc
    66781 tttcgcagtt tggcacccaa ctcgccccac tgtggttccc gacgtccatc atgatggtgg
    66841 cgttttatcg ccatgccggg cgcatgtggc cgggaattgc gctgagctgt tcgctgggaa
    66901 atatcgccgc atccatcctg cttttttcca ccagctcgct gaacatgacc tggacgacca
    66961 tcaatattgt tgaagccgtg gtcggggcag tgctgctacg taaattgctg ccgtggtata
    67021 accccttgca aaatctggct gactggctgc gtctggcact cggcagcgcc attgttccgc
    67081 ctctgttggg gggtgttctg gttgtcctgc tgacgcccgg agacgatcct ctcagggcat
    67141 ttttgatatg ggtactgtca gaatccatcg gcgctctggc actggtgccg ctgggattgt
    67201 tatttaaacc acactatctg ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc
    67261 tcacgttagc catcacactg acgttaagct ggctttcgat gctgtatctg ccgtggcctt
    67321 ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg cctgccacga atggaagcct
    67381 ttttgatctt ccttaccacg gtgatgatgg tgtcgctgat gatggccgcg gatccctccc
    67441 tgcttgctac gccgcgtacg tacctgatga gccatatgcc gtggctaccg tttttgctga
    67501 tcctgctgcc cgccaacatc atgaccatgg tgatgtatgc ctttcgtgcg gaacgcaaac
    67561 acatttccga aagcgaaacc cgctttcgga acgcgatgga atattccgct atcggtatgg
    67621 cgttagtggg caccgaggga caatggctgc aaaccaacaa agcgctctgc cagtttctcg
    67681 ggtacagtca ggaagagctg cgcggactca cctttcagca actgacctgg ccggaggatc
    67741 tcaataaaga tctccaacag gttgaaaagc tgataagcgg tgaaataaac acctattcaa
    67801 tggaaaaacg ctactacaac cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac
    67861 tggtgcgcca cacggatggc acgccgctct attttatcgc tcagattgaa gacattaacg
    67921 agctaaaacg caccgaacag gtcaatcagc aactgatgga gcgcatcacg ctggctaacg
    67981 aagcgggcgg gattggcatc tgggagtggg agttgaagcc gaatgttttt agctgggata
    68041 agcggatgtt cgagctgtat gaaattcctc cacatatcaa accgaactgg caggtgtggt
    68101 acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt gattcgttgc
    68161 aatcacgctc gccctttaaa ctggaatttc gcattaccgt aaaagacggt attcgccata
    68221 tccgcgccct cgccaaccgg gtactgaata aagaaggcga agtcgaacgt ctcctcggca
    68281 tcaatatgga tatgaccgaa gtgaaacagc ttaacgaggc attgtttcag gaaaaagagc
    68341 gcctgcacat tacgcttgat tccatcggtg aagccgtggt ctgtattgat atggcgatga
    68401 aaattacctt tatgaatcca gtggcggaga agatgagcgg ctggacgcag gaagaagcgt
    68461 taggtgttcc gctcctgacg gtgttgcata ttacttttgg cgacaacgga ccattaatgg
    68521 agaacattta cagtgccgac acctcacgtt ccgcaatcga acaagatgtg gtgttgcact
    68581 gtcggagcgg cggcagttac gacgtgcatt acagtattac gccgttaagt actctggacg
    68641 gcagcaatat tggttcggtt ctggtgattc aggacgtcac cgaatcacgc aaaatgctgc
    68701 gccagctgag ctacagcgcc tcccatgatg cactgacgca tctcgccaac cgcgccagtt
    68761 ttgagaaaca actgcgtatc ctgctgcaaa cggtaaacag tacgcatcag cgacatgccc
    68821 tggtgtttat cgatcttgat cgctttaaag cggtgaatga cagcgccggg catgcggcgg
    68881 gcgacgcttt gctgcgcgaa ctggcgtcgt tgatgctgag tatgctgcgc tccagtgacg
    68941 tgctggcgcg gctcggcggt gatgaatttg gtctgctgct gccagactgc aatgttgaaa
    69001 gcgctcgttt tatcgctaca cgtattatca gtgctgtgaa tgactatcac tttatatggg
    69061 aaggccgggt acatcgggta ggtgccagcg ccgggattac cttgatcgat gacaacaatc
    69121 atcaggcggc agaagtgatg tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg
    69181 gacggggccg ggtgacggtt tacgaaccac agcaagctgc cgcacatagc gaacgggcgg
    69241 cgatgtcgct tgatgaacag tggcggatga ttaaagagaa tcagttgatg atgatcgccc
    69301 acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt tcgcttaagc
    69361 tctggagttg cgaaggcgaa attattgatg aacaaacatt tcgtcgtagc ttcagcgatc
    69421 cggcacttag ccatgctctt gaccgacggg tattccacga atttttccag caggccgcca
    69481 aagcggttgc cagtaaaggc ataagcatcg ccctccccct ttccgttgcc ggtttgagta
    69541 gcgccacgct ggtgaatgat ctgcttgagc agctggaaaa tagccctcta ccaccacggt
    69601 tattacatct gattattccg gctgaagcga ttttagatca cgcagaaagc gtgcaaaaac
    69661 tgcagctggc gggatgtcgg atagtgctca gccaggtggg ccgcgatctg caaatcttca
    69721 actcgctgaa agcgaatatg gcagattacc tgctacttga tggtgagtta tgcgccaacg
    69781 tgcagggtaa tttgatggat gagatgctga ttacgattat tcaggggcac gctcagcgac
    69841 tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt agtgatggat acgctttctg
    69901 gcatcggcgt cgatctgatt tatggtgatg tgattgccga tgcccaaccg ctggatttgc
    69961 tggtgaatag cagttatttc gcgattaact gagagagcgt ttgcccattg cctgatgcga
    70021 cgctaacgcg tcttatcatg cctacaaatc gctcattccc cagaccggat aaggcgctcg
    70081 caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc cagccttccg tataccagat
    70141 atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc ggatttgtgc
    70201 cggtgtcatt cccggaaatc gccgtttaat cagataatca tccggcagaa aaacatcttt
    70261 cgcctgccag ccacgcaaag caaaataatt cgccgtccag cgcccgatac ccggaaaagt
    70321 ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta agctgccctc
    70381 cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca tgcctaacgc
    70441 ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc agatatactc
    70501 cggaaaatca tccagccgtt cgccataaag ctgtaccact ttggcagtca attttgccgc
    70561 catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa aagcatcaat
    70621 acagccgggt aaacgcaatc cgggccgccc cgcgcctaac tttcccaacg caccgttaac
    70681 aatctgcggg ttacattgca gatcaagcag gcggctcatt ttcgccagac actctgcggc
    70741 aacaggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta tatccggaat
    70801 agcagtcact acgccgcgat attcgcccac cgccagacta cgggcataat aattgtccgc
    70861 gaccgtttcc acaccgctca ccgcacgggc ggcgagaaat cccaacatcc acgaccagtc
    70921 atacggcggc tgccagttca gggtatacat cgcatctcct tgttaatccg ctttcagcat
    70981 aaacgttatt cagacgttac gctttgcttt catattccgg ttgtcgcgac agcaacattt
    71041 cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta ttggttttga
    71101 ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc aattactgaa
    71161 aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc gtgaatcggt
    71221 aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa cgcaggcgct
    71281 gttacgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg cgaaaagcgt
    71341 gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag tgtggtttgt
    71401 gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt
    71461 tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg cgcagctgcc
    71521 agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc tgggcggcga
    71581 tgaagcaaac gcccaggcgc aagagattct ggaacgcgcg gcgaagcgtg ccggattcag
    71641 ggacgtggta ttccagtacg agccgatcgc ggctgggctg gattacgaag ccaccttgca
    71701 ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt acgactgact gttcattgct
    71761 gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagtctgc tgggtcacag
    71821 tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa acctgatgcc
    71881 actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc tgccgtggtg
    71941 gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta gtgccaacgg
    72001 tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg ccctgttaca
    72061 gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag agtgcaaaat
    72121 tgctctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagca atgaactggc
    72181 tacgctgatt agccagcgag ggctggaaag cgccctcagc cagccactgg cgcggattct
    72241 ggaacaggtg caactggccc tggataacgc ccaggaaaaa ccggacgtta tctacctgac
    72301 cggcggtagc gcccgttctc cgcagattaa aaaagcgctg gcagaacagt tgccgggcat
    72361 tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac gctgggcgga
    72421 agtggtgttt cgttaattga aaaatggctc agcagcggct taaatctgcc tgcgcctgag
    72481 ccagtttttc tgctgcctga cgtaacggtt ccatcaacgg catggtccgt gctggagcat
    72541 gaaggcggaa tcgctgcaac tctgtcgcgc cgactgtcaa cgccgattcc agccgatgtc
    72601 gtttggcggt cattttcgcc attaccgctt gtctgtctgc tggcgttatc gcttcattcg
    72661 gcctgaacac aaaacggcgt tcacagttcg ctttccagtc gataaccgcc tgggtcagat
    72721 ggtcgccaaa ccctttcact tgcttaacgc cacgacgggt aacatctgcg gctgtttcaa
    72781 taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata gaggcaacat
    72841 caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcagtg tcatgaagtg
    72901 ctgccagagc gcgtttctct tcttccggta acccgagaat ttcgtccttc attttttcga
    72961 gcatcgtccg tttggcgata aaaccttcca ggccgcctac ctgctggatc tggcggacta
    73021 aatggtcata gtccattttc gcacgattaa atgcctcacg gcgttgctga acttctgctt
    73081 tgtacgcttt gcttgtcaaa ctgccaataa tccagatagc cgccagcaca ggaaccaaaa
    73141 taatataacg cggttctgcc tgaaggccgc aaagcagcag cgataatgct gacagtgcga
    73201 tctcaagcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc ctgccagtcg
    73261 gttggaaatg atcgggtaat ggcagttgca atgctggcgg tgctactgac gccatcacca
    73321 tcgcccagac ttttgccagc acaaaatccc cgccggtggt aatgacctct tcgccgagat
    73381 caataaaata gataacacct tgattatcca gcgcacacca tgggcagtca gccaaatgac
    73441 cgggataaac atgcattgcc gaaacggtac atttctttaa ctgttggcgt agagaatcca
    73501 gtgctgatac ccacgcttta gccgtcggac gcccggttgc cacgccactt tccgtaaacg
    73561 cctgctgaaa catggcttca acatcactcg gtaacatcga tagcggaatc gatcgtggcg
    73621 gcggttttaa accacgtcgc tgattatctg acgcgtaggc ataacggaaa tgggtaatat
    73681 ccgtctccag cgcattaccc gcatcagaga taagcggcac accagaataa ggatgccggc
    73741 caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga ttttctgtgc
    73801 gttcaaagcc gacaaatgac gacaaggttt gtagctctgg cggcgtaaaa tgcgacacgc
    73861 cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtcg ctatcaatca
    73921 acaccacttt gctgtcgcga cctaccataa aactgttctg gtttacgtca cccacgacgt
    73981 gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca tagagtagaa
    74041 aatcccacgc acaatgaggg taacgttgac gacgatgtgc cgggctatag atcatatgaa
    74101 tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg cctcgtccac
    74161 cgtgaagcgt tgcctgcggc cagacgacat aattcaacaa ttgcgcgtca gctgtcgcag
    74221 ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa atcttggcga
    74281 cgctatcgac aaactcattg atatcataaa ccgcgccttc accgcctttg cccagttcac
    74341 ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg gtaaccacag
    74401 cgccagtgcc aacgttttat catcatccgt acgctcgttt accgccgggc tggacaaaaa
    74461 ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgacg ccagtccatt
    74521 gaaaaacggg gtaaagaaag gcacatgagg agaattatcc agcatattta acgccagccg
    74581 ctgaatacca tccgtaaatg ctgcaacttt atgcgcacgc ccagtgctgg tgaaagtttc
    74641 cagtctggaa acggcatctt cgtcggtaat gaagtgcgtc atgttggcat attcacccac
    74701 catcggcgtg agaggcaatt gcagaccgtg acccaaatct accaccacgc cgccatcgcc
    74761 aatctgcata atcaaggtgc cgtcaggcga tgaaatcaat cccagaaacg tgcaggcaaa
    74821 atcgcgaact gctaatgctt tggcttctgc ctcggcaaaa agtcgttgac gaatagtcag
    74881 taccatattt gtggcgagga catcattaag ccccaattca ccgtcctgta ctttttgcac
    74941 cataaacgcc atcgcttcat tgacggcgag cgtcgcgcct tctcctccct gcgaaacgct
    75001 acctgcgcca tcagcaagga acatcaccag taatggttgc tggtcgttta accagacaac
    75061 ctgcatctgg caggcatcct gacaaggcaa atcagcacta atgtgcgatg ttccaacagc
    75121 ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc ttccagcaca
    75181 acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg aactggataa ccagctaaac
    75241 agttcacgga attgtaatcc ctgcagtggc aaaggctgac gaacgctgat ttgtgccaga
    75301 gtcttcatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt atcttcttcc
    75361 ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggagcgcc atcggtgatc
    75421 aggaaaatcc acgggcgata atacgaaata ccatttgcgc ggtactcacg tttcctctct
    75481 tcaaccatat ccagggcttt ggtaatggcg gcccccatag gcgtatcccc ctgggcaaac
    75541 aggatgggcg ggaaaaagtt agcggcgctg gtaaagggtt gttccacatg caccgggccg
    75601 aacgtcacaa tgccgagttc cactcttttt aaagccagcg gatcagcaag cagttcatca
    75661 cgaaaggtaa ccaatccggc gttaagttca ttaataggtc tgccgctcat cgagccagaa
    75721 acatccagca gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa atcgctggtg
    75781 gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattatcg aggtccggta
    75841 tcataccgat tgcaataatg aaataagagc aaacgaagaa actttcagaa ttaggcccgt
    75901 cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat tgacactaag
    75961 ggcggagtga cataatttca ggagtgaggg ttagggagag gtttccccct ccccctggtg
    76021 ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga cgtgcttcat
    76081 cataaccctt tccttattaa aagccctctt ctccgggaga gggtttcccg tttcagcgtc
    76141 ccgctgaaat cgtcggctta cctcctttcg ccctgaatgc agtctatcgc taacgcgtga
    76201 acaaaatagt ttcatctgtg attattgcgt aacgactcgc aaagaatatt tgctggcagg
    76261 atcgcagact acaaagcctg cggattgaca atcttatcgt gaaggcataa tttcaggagt
    76321 gagggtagag cggggtttcc cccgccctgg tagtcttagt aagcggggaa gcttatgact
    76381 aagagcacca cgatgatgag tagcttcatc atgacccttt ccttattaag gccccttcct
    76441 cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc
    76501 tgtatgcagt ctatcgctga cttgcggata aaatagtttc cagtgtgatt attgcgtaac
    76561 gtctcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcag attgacaatc
    76621 ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag
    76681 ccttagtaag ctggccagct aatgactaag agcaccacga tgatgagtag cttcatcatg
    76741 accctttcct tatttatggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct
    76801 gaaatcggcg gcttacctcc tttcgccatg caagcagtct atcgctaacg cgtagataaa
    76861 atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat tcagacgatt
    76921 cccgacagtg tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc
    76981 attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg
    77041 ttgggtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc aaggccgcaa
    77101 tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc cagcgggtgg
    77161 tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg ccgtagaaca
    77221 ggcagttccg cgttacctca ccgggcttgg caccattatc gccgctaata ctgttacggt
    77281 gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc agcaggtcaa
    77341 agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat tagcacaaac
    77401 ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg atctggcgcg
    77461 ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg cccaacaggc
    77521 gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg ccagcgcgca
    77581 gttgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg gtctcaagca
    77641 ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg tgatcaccca
    77701 gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta ctgtagtgca
    77761 ggcgcaaaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca attcgaagaa
    77821 attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa ccggtacgat
    77881 taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc agtttgttaa
    77941 cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag ccgccctgca
    78001 aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg tcagcaaaca
    78061 tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag gtatttctgc
    78121 gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga aagtggaagt
    78181 ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg aatacgcgaa
    78241 aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg cccgtcgcgc
    78301 ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt actcgccggg
    78361 attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta tccgaccatt
    78421 caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc cgttaccgcg
    78481 ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc gcaaagttcc
    78541 ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga tgtcgccgag
    78601 caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga tctgcctaac
    78661 ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct cgccgtcacc
    78721 tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt cgcgcagaaa
    78781 atctcgcaga tttccggcgt cggcctggtg acgctttccg gcggtcagcg tccggctgtt
    78841 cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga aaccgtgcgc
    78901 accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg cccttcccgt
    78961 gcagtcacgc tttccgcgaa cgaccagatg caatccgccg aagagtatcg ccagctaatc
    79021 atcgcctacc agaacggcgc gccaattcgt ctgggcgatg tcgcaactgt agagcaaggt
    79081 gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt gatgaatgtt
    79141 cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca gatgctgcca
    79201 cagctcactg agagtctgcc gaaatcggtg aaggtgacag tgctttccga tcgcaccacc
    79261 aatatccgcg catccgtcga tgatactcag tttgaattga tgatggctat cgcgctggta
    79321 gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc cggtgttgct
    79381 gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt ttctcgattt ttcaatcaat
    79441 aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga cgccatcgtg
    79501 gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc ggcggcgctc
    79561 aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcact gattgcggtg
    79621 ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga atttgctatt
    79681 accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac accgatgatg
    79741 tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc ccgtgcctcg
    79801 gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa agtgctgaat
    79861 catccgtggc tgaccttaag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg
    79921 gtgttcattc cgaaaggttt cttcccggta caggacaatg gcattattca gggcactttg
    79981 caggcaccgc aatccagctc ctttaccaat atggcccagc gacaacgcca ggtcgcggac
    80041 gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact
    80101 aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat
    80161 gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc
    80221 gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag ccgcacccag
    80281 taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt gccacagttg
    80341 atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggaccaa
    80401 gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg
    80461 gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat
    80521 actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac cccgggcctc
    80581 gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc gttaagctca
    80641 attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga tcagttcccg
    80701 gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggag
    80761 attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccagggc
    80821 agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg
    80881 gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt
    80941 ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc
    81001 gaactggatg tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac
    81061 gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc
    81121 gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg
    81181 gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt
    81241 ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc
    81301 acgccggtga tttatctgct gttcgaccgc ctggcattgt ggaccaaaag tcgctttgct
    81361 cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg
    81421 acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggcttccg tatgctgccg
    81481 gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt
    81541 gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc gcttgggcgc
    81601 attgccgggg tcagcgaaat gacctccagc agttcgctcg gcagcacacg tattattttg
    81661 cagtttgatt ttgaccggga catcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac
    81721 gctgcacaaa gtttgctgcc cagtgggatg cccagccgtc cgacctatcg caaagcgaac
    81781 ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa
    81841 ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgttggt
    81901 gatgtcgatg tcggaggcag ctcactgccc gccgtacgcg tcgggctgaa tccgcaggcg
    81961 ctgtttaatc agggcgtgtc gctggacgac gtacgcaccg ccatcagcaa tgccaacgtg
    82021 cgtaaaccgc agggcgcgct ggaagatggc actcaccgct ggcagatcca gaccaatgat
    82081 gagctaaaaa ccgccgctga atatcagccg ttgattattc actacaacaa cggcggcgcg
    82141 gttcgtctgg gcgatgtggc gacggtgacc gactcagtgc aggatgtgcg caacgccggg
    82201 atgaccaacg ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt
    82261 atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac cattccggcg
    82321 gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa
    82381 gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt tttattcctg
    82441 cgctcgggtc gcgccactat tattcccgcc gtttcggtgc cggtttcgct gattggtacg
    82501 tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat ggcgctcacc
    82561 atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat tgcacgtcat
    82621 ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga agtcggtttt
    82681 acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc
    82741 ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt
    82801 tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct gaaagccagc
    82861 aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc cctgcaacaa
    82921 ggctacggca agtcactgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg
    82981 cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac cttcttcccg
    83041 gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg atcagagtat ttcgtttcag
    83101 gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc ggcagtggat
    83161 aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt tatcaccctc
    83221 aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct gcgcgtaaaa
    83281 ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat tcgcgttggt
    83341 gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct ggcggcactg
    83401 cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact ggcggacgtg
    83461 aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg cgacaccatg
    83521 gcacggctgg gaatcgacgt gcaggccgcc aacagcctgt taaataacgc cttcggtcag
    83581 cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat ggaagtggat
    83641 ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa taacgaaggc
    83701 aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaacgctcc gctgtcggtg
    83761 aatcatcagg gattatcggc ggcctcgacc atctcgttca acctgccgac cggaaaatcg
    83821 ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt gccttcgacg
    83881 gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa ctcacaggtg
    83941 atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct ttacgagagt
    84001 tacgtacacc cactgacgat tctctccacc ctgccctcgg cgggcgttgg tgcgctgctg
    84061 gcgctggagc tgttcaatgc cccgttcagc ctaatagccc tgatagggat catgctatta
    84121 atcggcatcg taaagaaaaa cgccattatg atggtcgatt ttgcgctcga agcccaacgg
    84181 cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg ttttcgcccg
    84241 attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt gtcgggcggt
    84301 gacggctcgg agctgcggca ccccctgggg atcaccattg tcggtggact ggtaatgagc
    84361 cagcttctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg tctgcggctg
    84421 cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagaact tcccgacagc
    84481 acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct ggacaccacc
    84541 atcgttaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc gttgcatatg
    84601 cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc cagcggctgg
    84661 ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct gtttactctc
    84721 ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc acgcgcgtta
    84781 cagggcgttg gcggtgcgat gatggtgccg gtcggcaggt tgacggtgat gaaaatcgta
    84841 ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca ggtcggtccg
    84901 ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca ctggatcttt
    84961 ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgctgtt aatgccgaac
    85021 tacaccatgc agacgcggcg ctttgatctt tccggctttt tattgctggc ggttggcatg
    85081 gctgtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc gctggcgatt
    85141 gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca cgccagaaat
    85201 aacaaccgcg ccctgttcag tctgaaactg ttccgcactc gtaccttttc gctgggcctg
    85261 gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac accggttttc
    85321 ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat cccgatggta
    85381 cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg tgaatcgctt tggttatcgt
    85441 cgggtactgg tagcgaccac gctgggtctg tcgctgatca ccctgttgtt tatgactacc
    85501 gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg gatggtcaac
    85561 tcgacccgtt tctcctccat gaacaccctg acgctgaaag atctcccgga caatctggcg
    85621 agcagcggca acagtctgct gtcgatgatt atgcaattgt cgatgagtat cggcgtcacc
    85681 atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga cagcggcacc
    85741 acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc ccttccggcg
    85801 ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc gcggcgaaaa
    85861 aggagcgcgc aatgaagttc tggcgacctg gtattaccgg caaactgttt ctggcgattt
    85921 tcgccacctg cattgttttg ctgatcagta tgcactgggc ggtgcgtatc agttttgagc
    85981 gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg ttaagtgatg
    86041 cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac aatgatcgct
    86101 ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa cccggcccgg
    86161 gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac aacaaagtgc
    86221 tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt ctggtcaacg
    86281 gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc aatactgata
    86341 tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgccctg gcaacgttac
    86401 tcgcggcact tgccactttt ctgctggcgc gcggtttact ggcaccggta aaacgacttg
    86461 tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg cccaccagtg
    86521 aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcaca ctggagaaaa
    86581 accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt acgccattag
    86641 cggtgctgcg cggtgaactg gaagctattc aggatggcgt gcgcaaattc acgccggaga
    86701 cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac gatctccatc
    86761 agttgtcgat gtctgatgaa ggcgccctcg cctatcaaaa agcaccggta gatttgatcc
    86821 cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt ggcctgaaac
    86881 tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt ttaatgcagt
    86941 tattcaataa tttacttgaa aacagcctgc gctacactga cagcggcggc agcctgaaaa
    87001 tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt gcgccaggtg
    87061 tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa ggttcccgca
    87121 accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt gttgaagcac
    87181 ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt acagtagagt
    87241 taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg acgaaaacac
    87301 accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca ttgattatct
    87361 gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac tgccgtatgt
    87421 gcgccagaca ccaccggatc tgatcctgtt ggatctgatg ctccctggca ccgatggctt
    87481 gacgctgtgc cgggaaattc gtcgtttttc tgacattcct atcgtgatgg tgacggcaaa
    87541 aatcgaagag atcgaccgcc tgctcggact ggagattggc gcagatgact acatctgcaa
    87601 gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc gttgcaaacc
    87661 gcagcgcgaa ttgcagcaac aggatgccga aagcccgttg attatcgacg aaggtcgttt
    87721 tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc gtctgttgaa
    87781 aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca atcatcttta
    87841 tgacgactac cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa acctgcgccg
    87901 caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg gcgttggtta
    87961 ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact ttgttagccg
    88021 gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc aaactga
//
LOCUS       DAZRVY010000002        76181 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZRVY010000002 DAZRVY010000000
VERSION     DAZRVY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 76181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.002"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            56..379
                     /locus_tag="ACHXOX_000089"
     CDS             56..379
                     /locus_tag="ACHXOX_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR7003678.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(482..1732)
                     /gene="icd"
                     /locus_tag="ACHXOX_000090"
     CDS             complement(482..1732)
                     /gene="icd"
                     /locus_tag="ACHXOX_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR7003679.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1904..2557
                     /gene="rluE"
                     /locus_tag="ACHXOX_000091"
     CDS             1904..2557
                     /gene="rluE"
                     /locus_tag="ACHXOX_000091"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR7003680.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2567..3028
                     /gene="nudJ"
                     /locus_tag="ACHXOX_000092"
     CDS             2567..3028
                     /gene="nudJ"
                     /locus_tag="ACHXOX_000092"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR7003681.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3082..4188
                     /gene="mnmA"
                     /locus_tag="ACHXOX_000093"
                     /gene_synonym="trmU"
     CDS             3082..4188
                     /gene="mnmA"
                     /locus_tag="ACHXOX_000093"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR7003682.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4224..4865
                     /gene="hflD"
                     /locus_tag="ACHXOX_000094"
     CDS             4224..4865
                     /gene="hflD"
                     /locus_tag="ACHXOX_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR7003683.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4869..6239
                     /gene="purB"
                     /locus_tag="ACHXOX_000095"
     CDS             4869..6239
                     /gene="purB"
                     /locus_tag="ACHXOX_000095"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR7003684.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6407..7078
                     /gene="phoP"
                     /locus_tag="ACHXOX_000096"
     CDS             6407..7078
                     /gene="phoP"
                     /locus_tag="ACHXOX_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR7003685.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7078..8538
                     /gene="phoQ"
                     /locus_tag="ACHXOX_000097"
     CDS             7078..8538
                     /gene="phoQ"
                     /locus_tag="ACHXOX_000097"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR7003686.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8614..9735
                     /gene="roxA"
                     /locus_tag="ACHXOX_000098"
                     /gene_synonym="ycfD"
     CDS             8614..9735
                     /gene="roxA"
                     /locus_tag="ACHXOX_000098"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR7003687.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9784..11010)
                     /gene="pepT"
                     /locus_tag="ACHXOX_000099"
     CDS             complement(9784..11010)
                     /gene="pepT"
                     /locus_tag="ACHXOX_000099"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR7003688.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11260..12396
                     /gene="potA"
                     /locus_tag="ACHXOX_000100"
     CDS             11260..12396
                     /gene="potA"
                     /locus_tag="ACHXOX_000100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR7003689.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12380..13237
                     /gene="potB"
                     /locus_tag="ACHXOX_000101"
     CDS             12380..13237
                     /gene="potB"
                     /locus_tag="ACHXOX_000101"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR7003690.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13234..14028
                     /gene="potC"
                     /locus_tag="ACHXOX_000102"
     CDS             13234..14028
                     /gene="potC"
                     /locus_tag="ACHXOX_000102"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR7003691.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14025..15071
                     /gene="potD"
                     /locus_tag="ACHXOX_000103"
     CDS             14025..15071
                     /gene="potD"
                     /locus_tag="ACHXOX_000103"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR7003692.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15227..16048)
                     /gene="cobB"
                     /locus_tag="ACHXOX_000104"
     CDS             complement(15227..16048)
                     /gene="cobB"
                     /locus_tag="ACHXOX_000104"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR7003693.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16064..16975)
                     /gene="nagK"
                     /locus_tag="ACHXOX_000105"
     CDS             complement(16064..16975)
                     /gene="nagK"
                     /locus_tag="ACHXOX_000105"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR7003694.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17004..18248)
                     /gene="lolE"
                     /locus_tag="ACHXOX_000106"
     CDS             complement(17004..18248)
                     /gene="lolE"
                     /locus_tag="ACHXOX_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR7003695.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18248..18949)
                     /gene="lolD"
                     /locus_tag="ACHXOX_000107"
     CDS             complement(18248..18949)
                     /gene="lolD"
                     /locus_tag="ACHXOX_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR7003696.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18942..20141)
                     /gene="lolC"
                     /locus_tag="ACHXOX_000108"
     CDS             complement(18942..20141)
                     /gene="lolC"
                     /locus_tag="ACHXOX_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR7003697.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20403..21476
                     /locus_tag="ACHXOX_000109"
     CDS             20403..21476
                     /locus_tag="ACHXOX_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR7003698.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21604..25050
                     /gene="mfd"
                     /locus_tag="ACHXOX_000110"
     CDS             21604..25050
                     /gene="mfd"
                     /locus_tag="ACHXOX_000110"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR7003699.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25194..26150
                     /gene="ldtC"
                     /locus_tag="ACHXOX_000111"
                     /pseudo
     CDS             25194..26150
                     /gene="ldtC"
                     /locus_tag="ACHXOX_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26233..26490)
                     /gene="bhsA"
                     /locus_tag="ACHXOX_000112"
                     /gene_synonym="ycfR"
     CDS             complement(26233..26490)
                     /gene="bhsA"
                     /locus_tag="ACHXOX_000112"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR7003700.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26731..27363
                     /gene="comR"
                     /locus_tag="ACHXOX_000113"
     CDS             26731..27363
                     /gene="comR"
                     /locus_tag="ACHXOX_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR7003701.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27425..27964)
                     /locus_tag="ACHXOX_000114"
     CDS             complement(27425..27964)
                     /locus_tag="ACHXOX_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR7003702.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(28191..29495)
                     /gene="ndh"
                     /locus_tag="ACHXOX_000115"
     CDS             complement(28191..29495)
                     /gene="ndh"
                     /locus_tag="ACHXOX_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR7003703.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29903..30445)
                     /gene="ycfP"
                     /locus_tag="ACHXOX_000116"
     CDS             complement(29903..30445)
                     /gene="ycfP"
                     /locus_tag="ACHXOX_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR7003704.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30468..31493)
                     /gene="nagZ"
                     /locus_tag="ACHXOX_000117"
     CDS             complement(30468..31493)
                     /gene="nagZ"
                     /locus_tag="ACHXOX_000117"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR7003705.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31504..32328)
                     /gene="thiK"
                     /locus_tag="ACHXOX_000118"
     CDS             complement(31504..32328)
                     /gene="thiK"
                     /locus_tag="ACHXOX_000118"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR7003706.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(32309..32950)
                     /gene="lpoB"
                     /locus_tag="ACHXOX_000119"
     CDS             complement(32309..32950)
                     /gene="lpoB"
                     /locus_tag="ACHXOX_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR7003707.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32964..33341)
                     /locus_tag="ACHXOX_000120"
     CDS             complement(32964..33341)
                     /locus_tag="ACHXOX_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR7003708.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(33344..33703)
                     /gene="hinT"
                     /locus_tag="ACHXOX_000121"
     CDS             complement(33344..33703)
                     /gene="hinT"
                     /locus_tag="ACHXOX_000121"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR7003709.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            34037..36226
                     /gene="fhuE"
                     /locus_tag="ACHXOX_000122"
     CDS             34037..36226
                     /gene="fhuE"
                     /locus_tag="ACHXOX_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR7003710.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(36286..37719)
                     /gene="ptsG"
                     /locus_tag="ACHXOX_000123"
     CDS             complement(36286..37719)
                     /gene="ptsG"
                     /locus_tag="ACHXOX_000123"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR7003711.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(38014..38811)
                     /locus_tag="ACHXOX_000124"
     CDS             complement(38014..38811)
                     /locus_tag="ACHXOX_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7003712.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38822..39826)
                     /gene="holB"
                     /locus_tag="ACHXOX_000125"
     CDS             complement(38822..39826)
                     /gene="holB"
                     /locus_tag="ACHXOX_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR7003713.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39823..40464)
                     /gene="tmk"
                     /locus_tag="ACHXOX_000126"
     CDS             complement(39823..40464)
                     /gene="tmk"
                     /locus_tag="ACHXOX_000126"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR7003714.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40454..41476)
                     /gene="yceG"
                     /locus_tag="ACHXOX_000127"
     CDS             complement(40454..41476)
                     /gene="yceG"
                     /locus_tag="ACHXOX_000127"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR7003715.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41479..42288)
                     /gene="pabC"
                     /locus_tag="ACHXOX_000128"
     CDS             complement(41479..42288)
                     /gene="pabC"
                     /locus_tag="ACHXOX_000128"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR7003716.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(42408..43649)
                     /gene="fabF"
                     /locus_tag="ACHXOX_000129"
     CDS             complement(42408..43649)
                     /gene="fabF"
                     /locus_tag="ACHXOX_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR7003717.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43737..43973)
                     /gene="acpP"
                     /locus_tag="ACHXOX_000130"
     CDS             complement(43737..43973)
                     /gene="acpP"
                     /locus_tag="ACHXOX_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR7003718.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(44184..44918)
                     /gene="fabG"
                     /locus_tag="ACHXOX_000131"
     CDS             complement(44184..44918)
                     /gene="fabG"
                     /locus_tag="ACHXOX_000131"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR7003719.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44931..45860)
                     /gene="fabD"
                     /locus_tag="ACHXOX_000132"
     CDS             complement(44931..45860)
                     /gene="fabD"
                     /locus_tag="ACHXOX_000132"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR7003720.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45876..46829)
                     /gene="fabH"
                     /locus_tag="ACHXOX_000133"
     CDS             complement(45876..46829)
                     /gene="fabH"
                     /locus_tag="ACHXOX_000133"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR7003721.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46897..47967)
                     /gene="plsX"
                     /locus_tag="ACHXOX_000134"
     CDS             complement(46897..47967)
                     /gene="plsX"
                     /locus_tag="ACHXOX_000134"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR7003722.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(48048..48221)
                     /gene="rpmF"
                     /locus_tag="ACHXOX_000135"
     CDS             complement(48048..48221)
                     /gene="rpmF"
                     /locus_tag="ACHXOX_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR7003723.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(48273..48794)
                     /gene="yceD"
                     /locus_tag="ACHXOX_000136"
     CDS             complement(48273..48794)
                     /gene="yceD"
                     /locus_tag="ACHXOX_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR7003724.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48993..49577
                     /gene="yceF"
                     /locus_tag="ACHXOX_000137"
     CDS             48993..49577
                     /gene="yceF"
                     /locus_tag="ACHXOX_000137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR7003725.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49689..50648)
                     /gene="rluC"
                     /locus_tag="ACHXOX_000138"
     CDS             complement(49689..50648)
                     /gene="rluC"
                     /locus_tag="ACHXOX_000138"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR7003726.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            51221..54406
                     /gene="rne"
                     /locus_tag="ACHXOX_000139"
     CDS             51221..54406
                     /gene="rne"
                     /locus_tag="ACHXOX_000139"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR7003727.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54601..55554)
                     /gene="flgL"
                     /locus_tag="ACHXOX_000140"
     CDS             complement(54601..55554)
                     /gene="flgL"
                     /locus_tag="ACHXOX_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR7003728.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55566..57209)
                     /gene="flgK"
                     /locus_tag="ACHXOX_000141"
     CDS             complement(55566..57209)
                     /gene="flgK"
                     /locus_tag="ACHXOX_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR7003729.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(57275..58216)
                     /gene="flgJ"
                     /locus_tag="ACHXOX_000142"
     CDS             complement(57275..58216)
                     /gene="flgJ"
                     /locus_tag="ACHXOX_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR7003730.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(58216..59313)
                     /gene="flgI"
                     /locus_tag="ACHXOX_000143"
     CDS             complement(58216..59313)
                     /gene="flgI"
                     /locus_tag="ACHXOX_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR7003731.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(59325..60023)
                     /gene="flgH"
                     /locus_tag="ACHXOX_000144"
     CDS             complement(59325..60023)
                     /gene="flgH"
                     /locus_tag="ACHXOX_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR7003732.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(60076..60848)
                     /gene="flgG"
                     /locus_tag="ACHXOX_000145"
                     /pseudo
     CDS             complement(60076..60848)
                     /gene="flgG"
                     /locus_tag="ACHXOX_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(61020..61775)
                     /gene="flgF"
                     /locus_tag="ACHXOX_000146"
     CDS             complement(61020..61775)
                     /gene="flgF"
                     /locus_tag="ACHXOX_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR7003733.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61795..63000)
                     /gene="flgE"
                     /locus_tag="ACHXOX_000147"
     CDS             complement(61795..63000)
                     /gene="flgE"
                     /locus_tag="ACHXOX_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR7003734.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(63025..63720)
                     /gene="flgD"
                     /locus_tag="ACHXOX_000148"
     CDS             complement(63025..63720)
                     /gene="flgD"
                     /locus_tag="ACHXOX_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR7003735.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63732..64136)
                     /gene="flgC"
                     /locus_tag="ACHXOX_000149"
     CDS             complement(63732..64136)
                     /gene="flgC"
                     /locus_tag="ACHXOX_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR7003736.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(64140..64556)
                     /gene="flgB"
                     /locus_tag="ACHXOX_000150"
     CDS             complement(64140..64556)
                     /gene="flgB"
                     /locus_tag="ACHXOX_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR7003737.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64711..65370
                     /gene="flgA"
                     /locus_tag="ACHXOX_000151"
     CDS             64711..65370
                     /gene="flgA"
                     /locus_tag="ACHXOX_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR7003738.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65446..65739
                     /gene="flgM"
                     /locus_tag="ACHXOX_000152"
     CDS             65446..65739
                     /gene="flgM"
                     /locus_tag="ACHXOX_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR7003739.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65744..66160
                     /gene="flgN"
                     /locus_tag="ACHXOX_000153"
     CDS             65744..66160
                     /gene="flgN"
                     /locus_tag="ACHXOX_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR7003740.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(66200..67735)
                     /gene="murJ"
                     /locus_tag="ACHXOX_000154"
     CDS             complement(66200..67735)
                     /gene="murJ"
                     /locus_tag="ACHXOX_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR7003741.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67845..68768)
                     /locus_tag="ACHXOX_000155"
     CDS             complement(67845..68768)
                     /locus_tag="ACHXOX_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7003742.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68770..69417)
                     /locus_tag="ACHXOX_000156"
     CDS             complement(68770..69417)
                     /locus_tag="ACHXOX_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR7003743.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69428..70012)
                     /gene="rimJ"
                     /locus_tag="ACHXOX_000157"
     CDS             complement(69428..70012)
                     /gene="rimJ"
                     /locus_tag="ACHXOX_000157"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR7003744.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            70248..71456
                     /gene="mdtH"
                     /locus_tag="ACHXOX_000158"
     CDS             70248..71456
                     /gene="mdtH"
                     /locus_tag="ACHXOX_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR7003745.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71520..72167
                     /gene="grxB"
                     /locus_tag="ACHXOX_000159"
     CDS             71520..72167
                     /gene="grxB"
                     /locus_tag="ACHXOX_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR7003746.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            72301..72861
                     /locus_tag="ACHXOX_000160"
     CDS             72301..72861
                     /locus_tag="ACHXOX_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7003747.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72967..74013
                     /gene="pyrC"
                     /locus_tag="ACHXOX_000161"
     CDS             72967..74013
                     /gene="pyrC"
                     /locus_tag="ACHXOX_000161"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR7003748.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            74087..74332
                     /gene="dinI"
                     /locus_tag="ACHXOX_000162"
     CDS             74087..74332
                     /gene="dinI"
                     /locus_tag="ACHXOX_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR7003749.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74622..74876
                     /gene="bssS"
                     /locus_tag="ACHXOX_000163"
     CDS             74622..74876
                     /gene="bssS"
                     /locus_tag="ACHXOX_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR7003750.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74991..76109
                     /gene="solA"
                     /locus_tag="ACHXOX_000164"
     CDS             74991..76109
                     /gene="solA"
                     /locus_tag="ACHXOX_000164"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR7003751.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18565 a        20230 c        19025 g        18361 t
ORIGIN      
        1 aagaaatgaa catccaaatg aactatatta aataccgtgg gataagacat aacaaatgaa
       61 gtgggtagta attgacacgg taattcaacc tacatgcggc atatcttttt cagccatatg
      121 gggtgatatg aaaatgatca tctggtatca atctactata tttcttcctc ctggaagtat
      181 atttacaccg gttaagcttg gtattatcct taaggaaaaa gaatatccta ttactattta
      241 taacattgca ccattcaaca agaatttatg gagtttactc aaaagcagtc aagagtgtcc
      301 tccaggagaa agcaaaataa caaataaatg tttacataat agttgcatta taaaaatatg
      361 cccatatggg ctcaagtaat gggcttcata taaatatgct cgatatgcta ttaataatat
      421 ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac tcatggagag
      481 attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc ttagcgcctt
      541 ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct tccatacctt
      601 taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc tcagcggaga
      661 ggatgataga gcctggattt actttgtcct gaccggcata tttcggcgca gtaccgtggg
      721 tggcttcaaa cagggcgcat tcgtcaccga tgtttgcgcc aggggcgata ccgataccgc
      781 caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata caggcgataa
      841 catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca atcacgtctt
      901 taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg ccgccgtcga
      961 tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta aacgcgcctt
     1021 cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga tcgttagcaa
     1081 ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa cacggcttaa
     1141 taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct tcacgcagga
     1201 atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc gcataaatgt
     1261 cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta accgggcttg
     1321 gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt tcctggcgca
     1381 gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga cctttaatgg
     1441 caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca tcctgaccat
     1501 aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta cgctcgcctt
     1561 tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt acatctacac
     1621 cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg agtttgccgt
     1681 tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc attcacctct
     1741 ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt tgcgtcctgc
     1801 gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg tcaatgctac
     1861 cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc aattcataat
     1921 ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc gattcagctc
     1981 gcaacgttct actaggcgta aacctgaaaa ccagcccacg cgtgtgatcc tgttcaataa
     2041 accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat taaaagaatt
     2101 catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg aagggttact
     2161 ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca aacgcaccgg
     2221 gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg aagccttgcg
     2281 caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc tggttgacga
     2341 accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta ttcccaccag
     2401 ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca tgaccgccca
     2461 tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca ctttggataa
     2521 ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt ttaaaccgca
     2581 cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg aagagacgat
     2641 taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg aaaccttagt
     2701 ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac cgcaacactt
     2761 tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt tcctctttgc
     2821 cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg actgctgtcg
     2881 ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc tggtggcgga
     2941 aagtatccgt tgttatcaaa gtgggcaacg ctatccgctg gagatgattg gcgattttaa
     3001 ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc cgccttgaag
     3061 ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt cggcatgtcc
     3121 ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata tcaggttgaa
     3181 ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac agcggcagcg
     3241 gatctggctg atgcccaggc tgtctgcgac aagctcggca ttgaactgca caccgttaac
     3301 tttgctgccg agtactggga caatgtcttc gaactgttcc ttgccgaata taaagccggt
     3361 cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc tttcctcgaa
     3421 tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt acgtcgggcc
     3481 gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga ccagagctac
     3541 ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt cggcgaactg
     3601 gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc gaagaaaaaa
     3661 gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct gggccgttat
     3721 ctcccggggc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg tgagcaccag
     3781 gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg caccaaagaa
     3841 ggcaccgaag aaccttggta tgtggtggac aaagacgtcg aaaacaacat tctggttgtc
     3901 gctcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca gcagttgcac
     3961 tgggtcgatc gcgaaccatt caccggcact atgcgttgca cggtaaaaac ccgctatcgc
     4021 cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga agtgattttc
     4081 gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa cggtgaagtg
     4141 tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt attatcttta
     4201 cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc ctcgccctgg
     4261 ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg cattgtgatg
     4321 ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc tcgacgctgg
     4381 cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg ctcggcgtgc
     4441 tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca ctcagcttga
     4501 tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg ggcaaccgga
     4561 tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg ctgatgagcg
     4621 cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccacgcatt caggtcaccg
     4681 gttcccctgc tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca accctgctgg
     4741 caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg caactgatgt
     4801 tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta accccggagt
     4861 tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga cgctacggcg
     4921 ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa ttccgtgtac
     4981 aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag gaagttcctg
     5041 cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc agcgaagaag
     5101 atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa gcggttgagt
     5161 atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct gaattcatcc
     5221 actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg ctgaaaaccg
     5281 cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt aaagatctcg
     5341 ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca gccacgccgt
     5401 caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag taccgccagc
     5461 ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat aacgcccaca
     5521 tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc acctcgctgg
     5581 gtattcaatg gaacccgtac actacccaga ttgaaccgca cgactacatc gccgagctgt
     5641 ttgattgcgt tgcacgcttc aacaccattc tgatcgactt tgatcgtgac gtctggggtt
     5701 atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt tcttccacca
     5761 tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg ggcctttcca
     5821 acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag cgtgacctga
     5881 ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg attgcgtatc
     5941 aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg ctggatgagc
     6001 tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt cgctatggca
     6061 tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt gacgccgaag
     6121 gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc cgcctgaaag
     6181 cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag ctgaaataaa
     6241 ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta tccgcttttt
     6301 attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc cataaccaca
     6361 taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc gcgtactggt
     6421 tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg atgctggtca
     6481 tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg aacatttacc
     6541 ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac tgattcgccg
     6601 ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg aaagctggca
     6661 ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac catttcatat
     6721 tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc tggcttcaca
     6781 ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat ctattaatga
     6841 cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga tacgcaataa
     6901 tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg cggagctgcg
     6961 ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc aggcacaata
     7021 tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat tgcgctgatg
     7081 aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct gttggcaacg
     7141 gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat cggttatagc
     7201 gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct gttctatacc
     7261 cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat cgacaagcaa
     7321 agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc gcaacgtgac
     7381 gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg ttttcatgaa
     7441 attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc gatacagcaa
     7501 cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc ggtggcagta
     7561 aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt ggataccatt
     7621 ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt gctctcagcc
     7681 aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt acgccccatc
     7741 gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt gctcaatcca
     7801 gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt aaaaagtgaa
     7861 cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag tctgaaaacg
     7921 ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat gagcgtcagt
     7981 gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat tggctactac
     8041 ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca tccggtcgcc
     8101 ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa aggggtcaat
     8161 atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga ttttgtcgag
     8221 gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt cgaaatttct
     8281 gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc cggtattcca
     8341 ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt acgccctggg
     8401 caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg taaaatcgtc
     8461 gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg ccagcattct
     8521 gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca tccgttataa
     8581 tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac tcttaactgg
     8641 cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg cggctttaat
     8701 aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga aagcgaagtt
     8761 gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg cccgttcgaa
     8821 agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt gaaccactgg
     8881 catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg gcgtattgat
     8941 gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct cgatcagtac
     9001 gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga aaagctgcaa
     9061 atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga agccatcatc
     9121 gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc gcatgaaggc
     9181 tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa tacgcgggaa
     9241 ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa ctactacagc
     9301 gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga gatggataaa
     9361 ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca atggtttggc
     9421 gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccagaacc gccttatcag
     9481 ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct gggtggtctg
     9541 cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga ttccccgcac
     9601 cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa ttttggcgat
     9661 gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag cgggtattgg
     9721 ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg catccctttt
     9781 cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca ccgctttttc
     9841 cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc cagtgaacaa
     9901 gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccac cgcggatcgg
     9961 tttcagttcc ggttcaatat cgcagtcacg catcgcctgc tgggcgatat cgagaatatg
    10021 cggatgctca accactttct ctcgcatatt gtagtaactg tcttcaatca ccagttcaat
    10081 gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca ttttacgttt
    10141 acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat cggcccgttc
    10201 aacggtgcct ttcatgctcg ccaggtgata gaaaccttca tagccttctg tcatttccgg
    10261 gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg catttaccat
    10321 cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat tgaccgatgc
    10381 ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag tgtaagccca
    10441 gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt cttcatccgg
    10501 ggtaaaggcg actcgaatat caccatgcgg aatgtttttc tgttgcaata ccgccagcgc
    10561 ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt taccatcggt
    10621 ggtaatcaac gtctgaccca gtagctgatg cagcaccggg aacataaccg gtgataaaac
    10681 ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa tttgcggatt
    10741 aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc caatcgccgg
    10801 gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct cacttaaggt
    10861 cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata acttccattg
    10921 gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca gagatacgta
    10981 gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg tgacaacatt
    11041 attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg caagcgggaa
    11101 tatttatcag cattacctta atgaatataa agctaagcca gtaattcaca acaaagattg
    11161 gcgattcaag cggtttgccg tagaattacc gccctcatta agagtcacca aggtggttaa
    11221 ccacaaaccc cgcatcggta agccatccgt tgcgtttaca tgggacagag taaaaaattg
    11281 aataaacaac cgagttcgct ttcaccgctg gtgcaattgg cgggaattcg caaatgcttt
    11341 gatggtaaag aggtcattcc ccagctggat ctgactatca acaatggcga gttcctcacg
    11401 ctgcttggcc cttctggctg cggtaaaaca accgttcttc gcctgattgc aggtctggaa
    11461 actgttgatt ccggacgcat catgctggat aacgaggaca tcacccacgt tccggcggaa
    11521 aaccgctatg tgaacactgt tttccaaagc tacgcacttt tcccccacat gaccgtgttc
    11581 gaaaatgtgg cctttgggtt gcgcatgcaa aaaacccccg ctgctgaaat tacgccccgc
    11641 gtgatggaag ccctgcggat ggtgcaactg gaaaccttcg ctcaacgtaa accgcatcag
    11701 ctctctggtg gtcaacagca acgcgtcgcc attgctcgcg cggtggttaa caagcctcgt
    11761 ctgttgttgc tggatgagtc gctctcagcg ctggattaca aactgcgtaa gcaaatgcag
    11821 aacgagctga aagcgttaca gcgtaagctt ggtattacct tcgtctttgt cactcacgat
    11881 caggaagaag cgctcaccat gtcagacagg attgtggtga tgcgcgaggg tcgtattgag
    11941 caagatggta caccgcgcga aatttacgaa gagccgaaaa acctgtttgt tgccggcttc
    12001 attggcgaaa tcaatatgtt taacgccact gtcatcgaac gtctcgacga gcagcgcgta
    12061 cgcgccaacg ttgaaggccg cgaatgtaat atctacgtta acttcgccgt tgaaccgggg
    12121 caaaaactgc atgttctgct gcgcccggaa gacttacgtg ttgaagagat taacgacgac
    12181 aaccacgctg aagggctgat tggttacgtt cgtgagcgta actacaaagg catgacgctg
    12241 gagtcggttg ttgaactgga aaatggcaag atggtgatgg tcagcgaatt cttcaatgaa
    12301 gacgaccctg actttgacca ctctctcgac caaaaaatgg ccattaattg ggtagaaagc
    12361 tgggaggtcg tactggctga tgaagaacac aagtaagttc cagaatgtag tgattgtcac
    12421 tattgtcggt tggcttgtgt tgtttgtctt tctgcccaac ctgatgatca ttggcaccag
    12481 ctttttgacc cgcgacgacg ccagtttcgt caaaatggtc tttacgctgg ataactacac
    12541 gcgtctgctc gatccgctct attttgaagt gctattgcac tcgctgaata tggcgctgat
    12601 cgccaccctc gcctgcctgg tgctgggcta cccgtttgcc tggtttctgg cgaagctgcc
    12661 acacaaggtg cgtccgctgc tgctgtttct gctgattgtt cccttctgga ccaactcact
    12721 gattcgcatc tacgggctga aaattttcct cagcaccaaa ggctatctca acgagttttt
    12781 gctctggctg ggcgttatcg acacaccaat tcgtatcatg ttcacgccca gtgcggtgat
    12841 tatcggtctg gtttacattc tgctaccgtt tatggtgatg ccgctgtact ccagtatcga
    12901 aaaactggat aagccgttgc ttgaggcggc gcgcgatctc ggtgccagca agttacagac
    12961 ttttatccgt atcattattc cactgacgat gccgggaatt attgccggat gtctgctggt
    13021 gatgctgcca gcgatgggcc tgttctatgt atccgacctg atgggcggtg cgaaaaacct
    13081 gctgatcggt aacgtcatca aggtccagtt cctcaatatt cgtgactggc cgtttggtgc
    13141 agccaccagc attacgctga ctatcgtaat gggcctgatg ttgctggttt actggcgcgc
    13201 ttctcgtctg ctgaataaaa aggtggaact cgaatgatcg gtcgactgct tcgcggcggt
    13261 tttatgaccg ctatctacgc gtacctgtat atcccaatca ttattttgat tgtgaactcc
    13321 tttaacagct cgcgctttgg catcaactgg cagggtttta ccaccaaatg gtatagcctg
    13381 ctgatgaaca acgacagcct gttacaggca gcgcagcatt cactaacaat ggcggtgttt
    13441 tcggcgacat ttgctacgct tatcggttca ctgacggcag ttgcgctgta ccgttatcgc
    13501 tttcgtggta agccgttcgt tagcggaatg ctgtttgtgg tgatgatgtc accagatatc
    13561 gtgatggcga tttctctgct ggtgctgttt atgctgctgg gtattcagct tggcttctgg
    13621 tcgctgctgt tctcgcatat caccttctgc ctgccgtttg tggtggtgac ggtgtattcg
    13681 cgcctgaaag gttttgacgt gcggatgctg gaagcggcga aagatctcgg tgccagtgaa
    13741 tttaccattc tgcgaaaaat cattctgcca ctggcaatgc cagcggtggc ggcgggctgg
    13801 gtgttaagct ttaccctgtc gatggacgat gtggtagttt cttcgttcgt caccggacca
    13861 agttatgaaa ttctgccgtt aaaaatttat tcgatggtca aagtcggcgt atcgccggaa
    13921 gttaacgcgc tggcaaccat attactggtg ctgtcgctgg tgatggttat tgccagccag
    13981 cttattgctc gtgataaaac gaaaggtaac acaggggacg ttaaatgaaa aaatggtcac
    14041 gccacctgct cgcggcgggt gctctggcac tgggcatgag cgccgctcac gccgatgaca
    14101 acaacacgct gtatttctac aactggaccg agtacgtgcc gccaggactg cttgaacagt
    14161 tcaccaaaga aaccggtatt aaggctatct attcgactta cgagtcgaac gaaaccatgt
    14221 acgccaagct gaaaacgtac aaagacggtg cctatgacct ggtggttcct tcaacctatt
    14281 acgtcgataa aatgcgtaaa gaagggatga tccagaagat cgacaagtcg aagttaacga
    14341 acttcagcaa tctcgatcca gacatgctca acaagccgtt tgacccgaat aacgactact
    14401 ccattccgta tatctggggt gcgacggcga ttggcgttaa cggtgatgcg gtggatccta
    14461 aatctgtcac cagttgggca gatctgtgga agccagagta caaaggtagc ctgctgctga
    14521 ccgacgatgc ccgtgaagtg ttccagatgg cgctgcgcaa actgggctac tccggtaaca
    14581 ccaccgatcc gaaagagatt gaagctgcat ataacgagct gaaaaaactg atgccaaacg
    14641 tcgcagcgtt taactccgat aacccggcta acccgtacat ggaaggcgaa gttaacctcg
    14701 gcatgatctg gaacggctct gctttcgttg cacgccaggc gggtacgcca attgacgtgg
    14761 tgtggccgaa agaaggcggc attttctgga tggacagcct ggcgatcccg gcaaatgcca
    14821 aaaacaaaga aggcgcgctg aaattgatca acttcctgct gcgcccggat gtggcaaaac
    14881 aggttgccga aactatcggt tatccaacgc caaaccttgc ggcgcgtaag ctgttaagcc
    14941 cggaagtggc gaacgataaa acgctctacc cggatgctga aaccattaaa aatggtgaat
    15001 ggcagaatga cgttggcgca gccagcagca tttatgaaga gtattatcag aagctgaaag
    15061 caggacgtta attcagcaaa cgggcggtgg tattaccgcc cgttttttag ttagcaagga
    15121 tgtggctaat gagtcaggac agtaaagttt tatcccgctc tttcgtcggt attgtggtcc
    15181 ccttgtaggc ctgataagcg tagcgcatca ggcaatgctt cctgctttat aatcccttca
    15241 gcaacttttc aacaaactca ggcaccacct ggcttgccgg gccgtaatat ttctcggcaa
    15301 attcattacc gacctgactt ggttcgagat tcagttccac ggtgtgcgcg ccatgcagtt
    15361 tcgcttcgtg aacaaaccca gccgccggat aaacatgccc ggaagtacca atggcaatga
    15421 aaatatcagc catcgacaac gccatataaa tttcatccat gccgagtggc atttcgccaa
    15481 accaaacgac gtgcgggcgc aagggtgccg ggaactggca acaatggcat ttatcttctg
    15541 gcgtaacgtc tccggtccag tcgagaacct gaccactttg cgaacaacga actttcagca
    15601 attccccatg catatgaatg acattggtat tacctgcgcg ttcatgcagg ttgtctatat
    15661 tctgcgttac cagcaaaaag cgatcgccga gggcgtcttg cagtttagcc agcgcaagat
    15721 gcgcggcgtt aggctgaatt tctggctgct gcagctgtcg acgacgggcg ttataaaacg
    15781 cttgcaccag ttcaggatcg cgatcgaaac cttccggcgt tgccacatct tcaacccgat
    15841 gttcttccca caggccatct gcggcgcgaa aggtacgaat acctgattcc gcagaaattc
    15901 ctgcccctgt cagtacgagt actcttggtt tttccattgc ttccggcacc actttatctc
    15961 tgaaaaaaat acgctgacgc aaacgctcgc gcaggcggcg tttattttta cgaaaacgac
    16021 ttaaccgatg accccgacgc gacagcatag caacctctgt tgtttaatcg gttagatgta
    16081 ggaaggccgc accacgcatt cctcccgcat caccgtggcg cgcgcgttca atgcgcggaa
    16141 cacgagctac aggtaagaga tgacgaggca gcctgtccgc cagttgcgtt gtgattgccg
    16201 ggaaattcga taagccacca ccaatgacga ccaggtcaga gtcaacgatg gtcaggatat
    16261 ttcccagaca aaccgctaat aaatccagat aacgctcaac gtgcgccctt gcctgctcat
    16321 cgccttgatc ataaagcgca ataatttcgg gagcctgcaa cggttgatga taatagtgtt
    16381 gccagagcca cgcaaagccc cgacctgata gataattttc gatacaacca atttgaccac
    16441 agccgcagcg gcgtaacggg aaatccagcc ccatcatggt taacgcatca accggcagac
    16501 gcatatggcc aaactcgccg gtaatgtagc ttttaccggt aatcggttta ccgttgaaaa
    16561 tcagcccgcc gccaacgccg gtgccgagaa ttaaccccat caccagtgga tattgcgtaa
    16621 attcatcatc ccaggcttct gaaagggcaa aacagttggc atcgttatca aggcgtacat
    16681 cgcgatcaag acgtgcgctc aggtcggcac gcagcggttt accgctggca gcggggacat
    16741 tggcggcata cagcgtacca tcttctgttt ccggcattcc cggaataccg atgccaacag
    16801 aacctttaca gccaaaacgt tggtcagctt cggctaccag ctcgcatact gcatctaaaa
    16861 atgcgtcata gctgtcacgc ggtgtcggca cccgcttttc ccactgcaac tgccgaccgc
    16921 tatcaaacac gccaagcgca atttttgttc caccaatatc aaacccgtaa tacattgccg
    16981 ctccttgttt taatgtactg cctttactgg ccgctaagga ctcgcgcagg gtcaatatta
    17041 ctggcgcgcc gcgccggata ccaacttgcc aaaagactca gcaacaatgc tgtgaccagt
    17101 acgtagaaga cgtccagcca gtgcaattcc gatggcagga agtcaataaa atagatatcg
    17161 ctggagagga actgatgacc gatcagcttt tcaatccgct caataatcgg ggtaagttgc
    17221 agtgaaacaa ctacgccgat aatcacacca cacaggctac cgaacagccc tgccagcaat
    17281 ccataccaga caaagatggc gcgaattaaa ccatctttcg cccccagcgt tcttaatact
    17341 gcgatatcgc cactcttgtc tttcaccgcc atcactaagg tggagacgat gttgaaacag
    17401 gccacgccaa tcaccagtac catcgccaga tacataatgg cgcggatcat ctggatatcg
    17461 cgatacatat agccgtaagt accaatccaa cttttaatat aaacatagct gttggtcact
    17521 tcacccgcat cgcgtaccag cttattggcg ttgaaaacat ccgtcatttt aagggcaata
    17581 cctgacacgc tggaacccat atcaagatat tgttgggcat ccgccagcgg gatcatggca
    17641 aaactgtgat cgagttgacc actcaactgc aaaataccgg caatgtgcaa acgcacacgt
    17701 tttggctgca tcagtttatg ctcgggattc gagttgggga tcatgatcga cacccaatcg
    17761 ccctgcttca ctttcagcgc atccgccacg cctttgccga tgataatttg ctgttcgccc
    17821 gctttaaaat tgcgccaggc atccccctga acaaacgagg gtaatgcgct cagacgctgt
    17881 tcctgttgcg ggttaacgcc cttcacctga attgcgcgca gattggtacc actttctacc
    17941 agtccggtga aattgatata cggcgcagcg gcggcaatac ccggcacttt ttgcacgtta
    18001 tccagtgctt cctgccagtt agtccatggc tgatttaccg cctcaatttc accgtgcggt
    18061 accacagcaa gaatgcggtt attcagttcg cgttcaaagc cgttcatcgc gcttaagccg
    18121 acgatcaata ccgccacgcc gagggcaatg ccaatggtag aaatgacaga gattagcgac
    18181 accatgccgc cgcgtcgccg tccgcgacta aaacgcaggc caatcaataa cgataaaggc
    18241 atcgccatta ctccgccccc atcaggctca gttccgccgt cagacgtcca tcacgcattt
    18301 ccagttggcg gctcatacgt ttcgccagtt gcaggtcgtg agtaaccacc aggaaggcgg
    18361 tgccctgcaa gcgattcaat tccccaagca actggaagat gctgtcggcg ttacgcgcat
    18421 cgaggttacc ggtaggctca tccgcaagta ccagacgcgg attattgacc agcgcacggg
    18481 caatcgccac acgctggcgt tcaccgccag aaagttcaga tgggcggtga ttcgcacgat
    18541 gctccagccc caccgctttt aacatctcca gcgcacggct gttgatttca gcgggctttt
    18601 tcttgccaat cagcagtggc atagccacgt tttccagggc agtaaaatcc ggcagcaggt
    18661 ggtgaaactg ataaataaag cccagcttct ggttgcgcag ttctgctttc gccgccgaag
    18721 acagtttgct catcggttga ccgttaaaga tcacatcgcc ggaggttggt gtatccagcc
    18781 cgcccagcag gtgcagcaag gtacttttac cggaaccaga gctaccgacg atcgccatca
    18841 tttcaccttc gccgacgctg aaactgacat tgtgcagtac atcggtttgc acactgcctt
    18901 cctgatagcg tttgcacagg ttgtcgcatt gcaacaggat cttattcata acgtaaagcc
    18961 tcagcgggtt gagtggcggc agcgcgccat gaagggtaaa gcgtagacag cagcgcgata
    19021 gccatcgcca ccagcgcaat aacaatgacc tgtaaaggtt cgatagcgac cggcagcgcc
    19081 gcgccatcaa gcaggacgcc gattatcggc atcagattat ttaactggct ggcaagcagt
    19141 gcgccaagcg ccgctccgag gatcgcaccg ataatcccgg cgctggcccc ttgcaccata
    19201 aagaccatca tgatttgtcg cggagttaag ccttgcgttt gcaggatcgc gacttcgccc
    19261 tgcttctcca ttaccatcag ccctagtgag gtaataatgt taaacgccgc aacggcgaca
    19321 atcaggctca gcagcaagcc catcatattt ttttccatgc gtacggcctg gaacagctcg
    19381 cctttacggt cacgccagtc ctgccatttg ctgccttcag gcagtttttg ctgacttaaa
    19441 gagtcaactt tcagcggctc atccagccac aaacgccagc cggtaatatt gcctgccgga
    19501 taacgcatca gacgcgaggc atcctcaata ttcaccagca tttcatagcc atcgacttca
    19561 ctgttggcgg cgaaagtgcc aatcacattg aacaggcgct ggcttggaat acgccccatc
    19621 ggcgtgaact ggctggcaga tggtaccatc acgcggattt gatcaccgcg attaacgcct
    19681 agctgtgagg caagttgttc gccgaggatg acattatatt tccccggctc gaggtcagtt
    19741 tgtttcacat tgaccagata cggcgtaagt ggatcttttt gcgccgggtc gataccgagc
    19801 atcaccccga ccgccacgct gcgcgcgctt tgcagtacca catcaccggt agtaataggt
    19861 gcgacgcgat taacgccgtc cagtttgact gccgtttccg ggagttgctg cgggttaaga
    19921 gagccatgct cagaagagag aattgcctgt ggcatcaggc caaggatgtt gttttgcagc
    19981 tcgcgctcaa agccgttcat cactgacaat actgtgacca gcgccatcac cccgagggta
    20041 atgccgatgg tagaaagcca ggagacgaaa cgaccgaagc gatccgctgc acgcccacgc
    20101 atgtaacgca ggccaataaa tagagcgaca ggttggtaca tgaaatccgt ctggttgctg
    20161 tagcaaagac acgagtatat aagcgaaagc aagatgggta aacgtctgaa ctgtaagtga
    20221 tcgtcagggc taatctgaaa agcccccgta aatcaatcgg ctaaattgcc atgttttatc
    20281 atcaatcttg ccgcctgatc ttaatctgaa aaatcatcca atacagacag atacccatta
    20341 cacctcgggt cataacccgc aggatcaacc gcatgtaaat taaaaaagag cgtgttgtcc
    20401 tgatgaaaca aaaagagcta tggattaacc agatcaaagg gttatgtatt tgtctggtgg
    20461 tgatttatca ctcggtcatt accttttatc cgcatatgac cacttttcag catccgttat
    20521 cggaagtcct gagcaaatgc tggatctatt tcaatcttta ccttgcaccc tttcgtatgc
    20581 cggttttttt ctttatatcc ggctatttga ttcgccgcta tatcgacagc gtaccgtggg
    20641 gaaattgtct cgataaacgg atctggaaca tcttctgggt gctggcgctt tggggcgtgg
    20701 tgcagtggct ggcgttaagt gcgctgaacc agtggctggc acctgagcgc gatttaagca
    20761 atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg atgatcaccg
    20821 ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta tgtaaaattt
    20881 ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg gcggttaatt
    20941 tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg caatttgcct tattacagcc
    21001 ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg ttgcgccgcc
    21061 atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg tttactatct
    21121 cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat cagtacgagc
    21181 aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac accattgcta
    21241 tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt gcgcaaatga
    21301 acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat ccctttgtca
    21361 gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag cgtgcattca
    21421 gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct cgttaaacca
    21481 tcgccccagc cggggcgatt caatttgcta aaccatgtcg attacggata atgaacagca
    21541 ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa tctgacaacc
    21601 gttatgcctg aacaatatcg ttatacactg cccgtcaaag cgggtgagca gcgtctgctg
    21661 ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga acgtcacgcc
    21721 ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca tgatgaaatc
    21781 agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct tccctacgac
    21841 agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta ccagctaccg
    21901 acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg cgtttgccca
    21961 cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct gtcacgagat
    22021 gcattacgaa cccaactgga cagcgccggt tatcgccatg ttgaccaggt gatggagcac
    22081 ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag tgagctgcct
    22141 tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga cgtcgacagc
    22201 cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcgcacga atttccgacc
    22261 gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga agtgaagcgc
    22321 gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg gatcgagtac
    22381 tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt ccctgccaat
    22441 accttgctcg tgaatactgg cgatctggaa accagtgccg aacgtttcca ggctgacacg
    22501 ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc accacaatcg
    22561 ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg ggtgcagcta
    22621 aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca gaaactgcca
    22681 gacctggccg ttcaggcaca acaaaaagcg ccgctggatg cgctgcgtaa gttcctcgag
    22741 actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga agcgctgggt
    22801 gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga tgaagccagc
    22861 gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga taaggtgcgt
    22921 aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg ccgtcgtcag
    22981 gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga gctgcatatt
    23041 ggtcagccgg tggtccatct ggagcacggt gtcgggcgct atgccggaat gaccacgctc
    23101 gaagcgggcg gcattactgg cgagtatttg atgctcacct atgccaacga cgccaaactg
    23161 tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc ggaagaaaac
    23221 gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa agcggcggaa
    23281 aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc cgccaaagag
    23341 ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag cttcccgttt
    23401 gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttagcgacat gtgtcagccg
    23461 ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga agtggcgatg
    23521 cgcgccgctt tcctggcagt agataaccac aagcaggtgg cggtgttggt gcctaccacc
    23581 cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg gccggtacgt
    23641 atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct tgcggaagtg
    23701 gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag tgacgtcaag
    23761 tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt gcgtcataaa
    23821 gagcgcatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc aacgccgatc
    23881 ccacgcacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat cgccacgccg
    23941 cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct ggtggtccgg
    24001 gaggcgatcc tgcgtgaaat tttgcgcggg gggcaggttt attatctcta caatgatgtg
    24061 gaaaacatcc agaaagctgc cgaacggctg gcagaactgg tgccagaagc gcggatcgcc
    24121 atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga tttccatcat
    24181 caacgtttca acgtgctggt ttgtacaacc attatcgaaa ccgggatcga catcccgaca
    24241 gccaacacca ttatcattga acgcgcggat cacttcggtc tggcgcagct gcaccagtta
    24301 cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat ggttgctgac accgcatcca
    24361 aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct ggaagatctc
    24421 ggggcaggtt ttgcgctggc aacgcacgat ctggagattc gcggcgcggg tgaactgctt
    24481 ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat ggagttgctg
    24541 gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga tctcaccagc
    24601 cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt cattcctgac
    24661 gtgaatacgc gtttgtcgtt ctataaacgt attgccagcg ccaaaacgga aaacgaactg
    24721 gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc ggcgcgtacc
    24781 ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag gaagctggaa
    24841 ggtaatgaga aaggcggggt gatcgaattt gccgagaaga atcacgttaa tccggcctgg
    24901 ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc gacgcgcctg
    24961 aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg ccagtttatg
    25021 cgtgaactgg aagagaacgc gatcgcttaa tggtaaatcg ggggcgtttc tgcgccccca
    25081 tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt caccatccat
    25141 aattcaggct tatctgttta ttacaataac cttatattta ttattgattt ttggtgatga
    25201 tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc gtggcgctac
    25261 cggcaaaagc caacacctag ccgctgccgc cagcgagcag tcgtctggtt ggcgaaaaca
    25321 aatttcatgt ggtggaaaat gacggtggtt ctctggaagc catcgccaaa aaatataacg
    25381 tcggctttct cgctctgtta caggctaacc ccggcgttga tccttacgta ccgcgcgcgg
    25441 gcagcgtgtt aacgatcccg ttgcaaaccc tacttccaga cgcgccgcgc gaaggcattg
    25501 tgatcaacat tgcggagctg cgtctctatt actacccgcc gggtaaaaat tcggtaaccg
    25561 tgtatccaat aggtattggt cagttaggtg gtgacacgct gacaccgaca atggtgacca
    25621 ccgtttcaga caaacgtgca aacccaacct ggacgccaac ggcaaacatc cgcgcccgtt
    25681 ataaagcaca gggaattgag ttgcctacgg tagtgccggc tggaccggat aacccaatgg
    25741 gccatcatgc gattcgtctg gcggcctatg gcggcgttta tttgcttcac ggtacgaacg
    25801 ccgatttcgg cattggcatg cgggtaagtt ctggctgtat tcgtctgcgg gatgacgata
    25861 tcaaaacact ctttagccag gtcaccccag gcaccaaagt gaatatcatc aacactccga
    25921 taaaagtctc tgccgaacca aacggtgcgc gtctggttga agtacatcag ccgctgtcag
    25981 agaagattga tgacgatccg cagctgctgc caattacgct gaatagcgca atgcaatcat
    26041 ttaaagatgc agcacaaact gacgctgaag tgatgcaaca tgtgatggat gtccgttccg
    26101 ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca aactctgtaa atcctggata
    26161 aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat cagcagtagc aggcattaat
    26221 gagggttaat gcttatttat aaattactgc tgttccatgg agggtattcg gaccggttac
    26281 agaagtaata cggaaagatt ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc
    26341 cagcgatccc agatttgtcc ccgcgttagc actgatagta ccgacttttt gttggccttc
    26401 tggcgttgac tgaacttcga cagccgcaaa gttggcaaat gacatggagc ttaaaatcgc
    26461 cgcagcgatg agggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    26521 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    26581 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt taaatagaaa
    26641 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    26701 tcattcacag tcaccaggtt caacggcatc atggcaactg actcaacaca atgtgtaaaa
    26761 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    26821 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcgaccgga
    26881 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    26941 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    27001 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    27061 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    27121 ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    27181 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    27241 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    27301 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    27361 taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    27421 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    27481 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    27541 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    27601 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    27661 cccccacaac agtggcgaca tctttaccac gaccaccacc aaactgatgc cctatcacgc
    27721 cgccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    27781 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    27841 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    27901 ctgccgctac gcccagcgca gctgcgacac caatcccgat acccgccaac attgatttat
    27961 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    28021 ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaagga ttcggagaag
    28081 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    28141 ttgtccaggg cggatgaggc gtttatgcca catccgccag tgtacgtcga ttaatgcaac
    28201 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    28261 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    28321 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accgacagtg
    28381 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggt
    28441 ttaccgttca tctgcgccag aatattgttc attgcgcagg tcgccatctg gtgtgcagcc
    28501 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    28561 gcgtaaatgt ctggatcgcg ggtggtttgc agcgtcggtt ccaccaccag ctggttgata
    28621 cggttggttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    28681 cacaccatca gatcagcctc aatatattca ccatctttag tgtgcaggcc gccttcatca
    28741 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    28801 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    28861 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc
    28921 gcattgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    28981 ttcactttac cattcgcgcc caggttggcg gagtatttca ggaacagatt cagcatctcc
    29041 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct
    29101 ggcgtattga aatcgttaga ggtgctaccc agcgccatta ccagggtgtc ataggcgatt
    29161 ttacgttccg gaaccagcag ttcacctttc tcatcgcgca gttctgcaat agtgattgtt
    29221 ttcgcttcac gatcaatatc aatgacggaa cccagctgga actggaaacc atgattgcgc
    29281 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    29341 tgcagcagcg gtttccacag atggctgtgg ttacgatcga ccagcgtaat tttggctttt
    29401 ttcttgcgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    29461 ccgacaatca caattttttt caatggcgta gtcaacgtga cccccttaaa tttattaacc
    29521 aattgttaat taatggttat aaacatagct cttaattaac aacaggttac agcaatgaaa
    29581 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    29641 atcaagtttt gttgctgaaa agataggcga ctttaccggg tgagatcaaa aaacagagtt
    29701 tattgtttat tatttgagca agtccggaag ggtgatatag ggtgacgcgt atatcgaggg
    29761 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    29821 atgcggcgta aacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    29881 ttacctgatg cgacgagggc atttacccga gggttttgaa cgccttaatg cgctgcaaat
    29941 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    30001 gatgcaactc ttcagatgtc cgctggctgt taagcgcttc atcattacgc gacaaaatca
    30061 ccaggcaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    30121 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    30181 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    30241 gaccaacgcc gcaaatcagc ggacgctcgt cgacgttcag ttgcaacatt ttgtccactt
    30301 ctttgagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    30361 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    30421 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctgct
    30481 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc cttccagcga
    30541 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    30601 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    30661 accaggatca tatcgcaacc tgcgtccagt gatgcctgcc cgcgttcggc ataactgccc
    30721 ataatcgcgg caccttccat cgataaatcg tcagagaaaa ttacaccgtc aaaacacagt
    30781 tcctgacgca aaacggtttt cagccagtag gaagaaccgc tcgccggacg cggatcaaca
    30841 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    30901 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    30961 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    31021 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    31081 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    31141 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    31201 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    31261 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    31321 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    31381 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    31441 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    31501 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    31561 atcgccgatt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca gcagccaggg
    31621 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    31681 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    31741 cgcgatatca ccatctccgg catactccca gtcgatgagt tttaaccctg acgcgctatg
    31801 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gccgtggttc
    31861 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    31921 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    31981 ttgttgatga agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    32041 taccgcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    32101 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    32161 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    32221 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    32281 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    32341 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    32401 tgccagaggc gctggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    32461 ctttactacg ggtacctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    32521 acagctgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    32581 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    32641 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    32701 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt tgatcttcgt
    32761 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    32821 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    32881 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    32941 tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt gtgcgccccc
    33001 agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatgct aacgctgcgt
    33061 ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa acgataatga
    33121 acggtaacgg gttcttgcct ttcgttataa agcgttgagg atgctgaagg ttgaatatca
    33181 gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga tgactccatc
    33241 accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc caccagcagt
    33301 aacaacgcca gagacaccag cccaaagcat ccttttctca tcgttacaga cctttatgcg
    33361 ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg taaacctctt
    33421 gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca ataccttctt
    33481 gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca tgctcagctg
    33541 acacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc gttggcgctt
    33601 gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata tcggagggga
    33661 tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt tcctttttca
    33721 aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataga gtatggtcga
    33781 gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc gttttgctgc
    33841 cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt
    33901 ttggtttaca cgacaaatat gaatgcgcat atttctcatt tgcatttaca aacaaaatta
    33961 ttcgcacact aaatataacc gctcatcacc ctcctccggc atgagcctga cgacaacata
    34021 aaccaagaga tttcaaatgc tttcaacaca atttaacagg gataatcaat atcaagccat
    34081 caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt ctgccgcttt
    34141 tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag ctccagatga
    34201 tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc agatgactca
    34261 acttgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag atcagcagtt
    34321 acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc aggcggattc
    34381 cgatcgtgct ctttattatt cccgtggatt ccagatcgat aactatatgg ttgatggtat
    34441 ccccacctat tttgaatcgc gctggaatct gggcgacgca ctttctgata tggcactctt
    34501 tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg gtaatccatc
    34561 tgcggcaatt aatatggttc gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc
    34621 ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa gcccactcac
    34681 cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg actcatggct
    34741 ggaccgctac aacagtgaaa agaccttctt ctcgggcatt gtcgatgctg atttaggcga
    34801 tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata gcccaacctg
    34861 gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc gtgcacgcag
    34921 taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta tgaccctgaa
    34981 gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg aagtcgaatt
    35041 tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta tgctggttgg
    35101 gccatacagt aattatggac ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg
    35161 caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat tgtttggtcg
    35221 tcagcacaat ctagtgtttg gtggcagtta cagcaaacaa aacaatcgtt acttcagttc
    35281 atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg gcaatttccc
    35341 acaaaccgac tggtcaccac agagcctggc acaggacgat accacacata tgaaatcgtt
    35401 atatgctgct actcgagtca cccttgccga tccgctgcat ctgatcctcg gcgcacgtta
    35461 taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca ccacgcctta
    35521 cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct atacctctat
    35581 tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa tcaccggtaa
    35641 caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca ccacgttagc
    35701 catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta tccccggcag
    35761 caacggcgaa accgcctata aagcggtgga tgggacagtc agtaaagggg tggaatttga
    35821 actcaacggc gcaattaccg acaactggca gctgacattt ggcgcaacgc gctatattgc
    35881 agaggataac gaaggaaacg ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt
    35941 caccagctat cggttgcctg tcatgccaga gttgacagtc ggcggtggtg ttaactggca
    36001 aaatcgcgtg tataccgaca ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag
    36061 ctacgcgctg gtggatctct tcacccgcta ccaggtgacg aaaaacttct cgttacaggg
    36121 gaacgtcaat aacctgttcg acaaaaccta cgataccaac atggaaggtt ctatcgtcta
    36181 cggcgcaccg cgtaatttca gcattaccgg cacgtatcaa ttctgaataa cacctgtaaa
    36241 aaaggcagcc atctggcggc cttagtctcc ccaacgtctt acggattagt ggttacggat
    36301 gtactcatcc atctcggttt tcaggttatc ggatttagta ccgaaaatcg cctgaacacc
    36361 agaaccagca accactacgc ccgctgcgcc cagtttcttc aggccagcct gatccacttt
    36421 agacacatca gcaacgctga cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt
    36481 ttctttacca ccaaatgcag caaccagagc cggtgccatt tcgctggtac ctgttgcttt
    36541 tgcatcttca gtcgcgtctt cacgacccgg cgttttcaga tccagtgctt taatcagcac
    36601 gcggaagatg gtgtagtaaa caatcgcata accgataccg acgatcggga acagccacag
    36661 tttgctgctg ttaccagaca gaacgataaa gtcgatcaga ccgtgcgaga acgacgtacc
    36721 gtcacgcatc cccagaagaa tacagattgg gaatgccagg cctgccagaa tcgcgtggat
    36781 gatgtacagg atcggcgcaa cgaacatgaa ggagaactcg atcggctcgg tgataccggt
    36841 caggaacgag gtcagcgccg cggagatcat aataccgccc actttcgcgc ggttttctgg
    36901 tttagcagag tgccagatag caattgcggc agctggcaga ccgtacattt tgaacaggaa
    36961 gccaccagac agtttacccg cagtcgggtc acccgccata taacgcggaa tgtcgccgtg
    37021 gaaaacctga cctgctgcgt tggtgtattc accaatctgc atctggaaag gtacgttcca
    37081 gatgtggtgc agaccaaacg gcaccaggca acgttcgatg aaaccgtaaa tgccaaacgc
    37141 aactaccggg ttctggtaag cagcccactg agagaaggtc tggattgcag aaccaatcgg
    37201 cggccaaatg aaggacagca caacgccagt aaagatggca gccaggccag aaatgatcgg
    37261 cacaaagcgt ttaccggcaa agaagccaag atactcaggc agcttaatac ggtagaaacg
    37321 gttaaacatg tacgctgcga tcgcaccgga gataatccct ccgagtacgc cagtatccgc
    37381 caggtgttta gaggcgattt cttcagcagg taaatgcagt accagtggcg caaccacggc
    37441 catggtttta accatgatgc cataggcaac aactgcggcc agcgcggata cgccatcgtt
    37501 attggtaaag ccgagggcga caccgatcgc aaaaatcagt ggcatgtttg caaagacgga
    37561 accgcctgct tctgccataa catgcgatac aacggcgggc agccagctga aattcgcgga
    37621 accgacgccc agcagaatac ctgcgatagg cagtacggat accggcagca tcagcgattt
    37681 accgaccttt tgcaggttag caaatgcatt cttaaacata attgagagtg ctcctgagta
    37741 tgggtgcttt ttttacgttc tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg
    37801 catctaagcg ccctttattt attacacaga gtaaaataat tcagtgccga tttgtttgac
    37861 ggctatcacg tttcaacttc agatcattgg agaagttgca cattttgaca aaacatatgt
    37921 cacaatattt caaacgtggt tattcaccgc ccaattttcg gcggtgaact ttactcgttt
    37981 tagccattaa ttacgagctt taaaaaaact cattcaacgg atggattgaa ggcgggaagc
    38041 gtcgatgtga aacagacggg cgaagttatc ggtggttacc tgcgccagtt cttcaacggc
    38101 aacacctttc aacacagcca tgtattctgc aacgtcacga accatcgcag gttgattctc
    38161 ttttcctcga tgcggtaccg gcgcaaggta aggtgagtca gtttccacca gtaaccgatc
    38221 cagggggaca taacgcgcag catctcgaag ttgctccgca ttacggaagg tcacaatgcc
    38281 ggaaaaggag atgtaaaatc cgagatccag taatttaccc gccgtttctc tgtcctctgt
    38341 aaaacagtgt agtacgccac cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt
    38401 atcggcgcgg gcgtcacggg tatggacgat aaccggcttg ttcagttcac gaccaatctg
    38461 gatatgatgg atgaaggact cttgctgacg aactttagtt tccggtgtgt aataataatc
    38521 cagcccggtt tcacccagcg ctacaacacc ctcttctgcc gccagacggc gtaaatcttc
    38581 tacatcgtag ggatcattct ggttgagtgg atgcacgcca caagaaaata cgacgttgtc
    38641 acgttcgcct accagatccc gcatatgtaa ataacccggt aatgttgtgg cgactgccag
    38701 acaaaatttc acatcgcgtg cggcggcttt cgccagaacg tcatccacgt ccttatgcaa
    38761 agattcataa tccagaccat cgagatggca gtgtgagtcg actaaaaaca tgatgtctct
    38821 cttaaagatg aggaaccggt agcacaacgc ccggttgcag gtaatgctca atacgcagta
    38881 aaagatcggt gatgagaagc tcgcggttga tgcctgtgac agacattaac tgttcacgaa
    38941 tgtggcaaac atcccccagt atagcctgca ggcgcgaggg agaaagatgg tttgccagtt
    39001 cgacgacaag gcccggcaca tcaacattgg tcacctgcgc agcaccatga tggcgtttta
    39061 gcgcatccat cagcaacgtt gccagccagt gtaaacgcgc cggagcttgt tcatgattaa
    39121 gggccgccag cagcgaatac cagtcgcccg atggcacgct atatgccaat gcctgacaca
    39181 atgtttcacg agcctgccag ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac
    39241 cggcgcttaa gcgcaatgcg gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa
    39301 gccaggtcac ggcgtactgt tccggcggcg gcgcaaggta atgtaaccga caacgactac
    39361 gtaatgttgc cagtaaacgt tcaggctcgc gggtagccag gaaaaaccag gtttctgctg
    39421 gtggctcttc aagcgttttc agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat
    39481 cggttaccca gacaactttc gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga
    39541 cctcacgtac tgcatcaatg cccagcgtat ttttcccttt ttcgggagcc agggtgtagt
    39601 aatcgggatg cgtgccagcc tgcatcaact gacatccacg acagtgaccg caacttttgt
    39661 ggccctgcgg ttgttggcag agtaaataac ggctcagggc gtagattaaa gcatcatcgc
    39721 ccatgcccgg taacgcctga atgagtagcg catggtgacc tcttccggcc tgatagctgg
    39781 ctaccagttt ttcgaaatca ggtcgtaacc atggatacca tctcatgcgt ccaactcctt
    39841 cacccagtgg gtcacggtag tgcggattgc atccatcacg gcctccagcg gctgggtggc
    39901 atcaatggta tgaatacttt tatcttgtgc agccagttcc agatagcggg cgcgggtgcg
    39961 attaaagaaa tcgaaagact cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg
    40021 ttttaagcca acttccgggg taacatcgag atagagcgtt aagtcggggc gaaaatcccc
    40081 gagaacagca tcacgcagtg ttgccagcat atgttggtca ataccacgtc ctccgccctg
    40141 atacgcctga gtggagagat cgtggcgatc gccaatcacc caggtgccgt tagccagagc
    40201 tggtttgatg accgtttcta ccagttgaac gcgcgcggca taaaacatca gaacttcggc
    40261 tttatcggta atgacttcat cgcctaccga tttgatatcc agcaccaggc ttcttaactt
    40321 ttcggcaagt tgcgtaccgc caggttcccg agtgaaaacc atgtcgcgga tacccagttg
    40381 ctcaagcgtc tcaaccacca cattacgcgc ggtagttttg cctgcgcctt ccagcccctc
    40441 aatgacgata tacttactgc gcattttttt ccttaagcac tttcagataa tcctgcacag
    40501 acttgttatg gctggcaaga ttggtattaa acgtgtgacc acctttacca tcggccacaa
    40561 aatagagata cggcgttttt gccggatgcg cagcagcctt cagcgaatcc gcccccggcg
    40621 tagctatcgc acccggcggc aaaccggtaa tggtataggt gttatacgct gtcggcgttt
    40681 ccaggtctgc acgagaaagt ttgccattat aacgctctcc catcccgtaa atcacggttg
    40741 ggtcggtctg caggcgcata ccaatgcgta aacggttgat aaataccgag gcaacctgat
    40801 cgcgttcact ggcaacggcg gtttcttttt cgataattga tgccatcgtc accagctgat
    40861 ttttatcttt ataaggcaga ccgtccgcac gcccttccca ggcgctatcg accgctttca
    40921 ccattttctt gtgcgctcgc ttgagtaacg cgacatcggt ggtattggcg gtatacatcc
    40981 aggtgtctgg ccagaaccaa ccttcaatcc actccgggtt ttccagttca agtgcctgcg
    41041 ctacggtggc gtacttatcg tcactcagcg tgtgcttgat atacggagcc tcacgcaatt
    41101 gcttgaggta gtcgctcaga cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt
    41161 taccgctttc cagcaatttc agcatctcgc gcacggtcat ctgcggtgta aagcggtaag
    41221 tcccggcttt aaagtgagaa agatccggtt cgatacgcag caaccattga aaaacccgtg
    41281 ggcgattgat gatcttatcg gcataaagct gttcaccaag cgccagacgt ccggtccctg
    41341 gcttcagggt aaatatcgtc tcttctttga taagcaattt gctgtcggca agatggcgaa
    41401 ccttccagac gcccacacca gcggcgatac ccagtaccac cagcaataac aagataatca
    41461 ataacacttt tttcatgact aattcgggcg ctcacaaagt ggggctaaat attcatataa
    41521 cgttgctgac gaaaaggaga catcgccaca ggcacgtacg ggcatcactg gcattaacgc
    41581 attacaaata accatctcat ctgcctgcaa cgcctcttcc agagaggctt gcacttcgac
    41641 aagctgataa gaggattgtg ccagcaaacg gatacagaat tgtcgcataa tgccgttaac
    41701 acctgcctga tccagacgcg gcgtataaac tacattgccc ttccgccaga acaaattagc
    41761 cgcacagcat tccgtaaccc acccttcgct gtcaaggacc agcgcctcat cagcgtttgt
    41821 ctgctcaaga tgagagcgga tcaatacttg ctcaagccga ttaagatgtt taatacctgc
    41881 aagatgagga ttgcgcccca gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg
    41941 caaacggtcg taatgtgcag gataaaccgt aacggagaga atccgcgttg ccggtccact
    42001 gttcaatgtg ctgtaccctc gcccgccact accgcgactg atcacgactt tcagcacacc
    42061 attttgctgt tctgctgcca gcgttttcat ctcttgttca agctgaggcc agaagtcaca
    42121 gaaaatcatc aaccgctgac aagcatcctg tagtcgctgg atatgcgccg ataacaaact
    42181 gactttaccg tcgataactc tggcggtggt aaaacaacca tcaccaaact gcgttgcccg
    42241 atcgcttact gccagcgatt cctgcttatg accgttaatt aagaacatac cggctcctta
    42301 tcagagaatt gttagtgtgg cagcatgttc actacggaac aagtcggaat aaaagctaag
    42361 aaaaaaggcc cgcaagcgga ccttttatat gggtgggaaa tgacaactta gatcttttta
    42421 aagatcaaag aaccattagt gccaccgaag ccgaaggagt tacacagcgt gtattccatt
    42481 ccgctgacct gacgtgcttc atgcggtacg aaatccagat cgcaaccttc atccgggtta
    42541 tccaggttga tggttggcgg aacagcctga tcgcgcagcg ccaggatgga gtagatagat
    42601 tctactgcac ccgccgcacc taacaggtga ccggtcatag atttcgtgga gcttaccaac
    42661 acacggcttg cagcttcacc gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca
    42721 gccggcgtag aagtaccgtg cgcgttaacg tagccaatct gactcgcttc aatgcctgca
    42781 tcacgcagag catttgccat cgccagagct gcgcctgcgc cattttctgg cggtgacgtc
    42841 atatgataag catcgctgct cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg
    42901 cgttttttcg cgtgttcgta ctcttcaagt accagcatac cggcaccatc gcccagtacg
    42961 aagccatcac gctctttatc ccacgggcgg ctcgccgctt gcgggttatc attgcgggta
    43021 gataatgcac gtgccgcgcc aaaaccacca acacccagcg gcgtactggc tttctctgcg
    43081 ccacctgcaa ccatcacgtc agcatcgcca tacgcgataa tacgcgcagc atggccaatg
    43141 ttgtgcacgc cggaagtaca ggcggtcgcg atagagatgc ttgggccacg caggccatac
    43201 atgatagtca gatgacctgc caccatgttc acaatcgttg acggaacgaa gaatgggctg
    43261 atcttacgtg gaccaccgtt catcagagat gtgtggtttt cttcgatcag tccgaggccg
    43321 ccaatcccgg agccaattgc ggcaccaatg cgggttgcgt tctcttccgt tatatcaagg
    43381 ccagaatcct gcatggcctg aacgccagcg acaattccat attgaatgaa ggcatccatc
    43441 ttgcgctgtt ctttgcgcga gataatgtcc tcacagttaa aatcctttac taagccagca
    43501 aatttcgttg cataggcgct agtatcgaaa tggtcgatta ggctgatgcc actctgaccg
    43561 gcaagcagag ctttccaggt agactctacg gtattgccga caggagacaa catgcccagt
    43621 ccggtcacaa ctacacgacg cttagacacg tttgtcctcc agggagggaa aaaatgattc
    43681 tagtgggaca aaaagataaa actcaggcgg tcgaacgacc gcctggagat gttcacttac
    43741 gcctggtggc cgttgatgta atcaatggca gcctgaacgg tggtgatttt ctcagcttct
    43801 tcgtccggaa tctcagtatc aaactcttct tccagagcca ttaccagctc aacggtgtca
    43861 agagaatccg cgcccaggtc ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta
    43921 acgcccagct gttcgccgat aattttctta acgcgttctt cgatagtgct catactctta
    43981 aatttcctat caaaactcgc tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat
    44041 ttgcaactaa atcccggcag gtcttaccac gattttacgt tattttgagg ccttaccccc
    44101 caataacgca aatcattttg cactaattat tactcataac cacgcagact gcgcgcaaca
    44161 tgagcatttt gtgcaaatcg cggtcagacc atgtacatcc cgccgttcac atgcaaagtt
    44221 tcacccgtga tgtaagctgc ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc
    44281 tgtgcgccgc cgaggcgacc cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg
    44341 ctcagcgcac gtgtcatgtc cgtttcaata aagcccggag caacaacgtt tacagtaata
    44401 ccgcgtgacg caacttcgcg cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc
    44461 gcagcgtagt tggcctgacc gccatttccc atggtaccaa ccacagaacc gatagtgata
    44521 atacgaccat gacgcttttt catcatagcg cgcattaccg cttttgacag acggaaaaca
    44581 gatgaaaggt tggtttcgat aatatcgttc cactcttcat ctttcattcg cattaacagg
    44641 ttatcacgag tgataccggc attattgacc aggatatcca cttcaccaaa ttctgcgcga
    44701 attttttcca gaacagattc gatagatgcc gggtcggtca cattcaacat cagacctttg
    44761 ccgttggcac ctaaataatc gctgatcgcc tgagcgccat tttcactggt cgcagtgcca
    44821 ataactttcg cgccacgggc tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt
    44881 gcaccggtta ccagtgcgat ttttccttca aaattcatga ttttcctctt ttaaagctca
    44941 agcgccgctg ccatcgctga aggttcgttc agcgccgagg cggtcagggt gtcgacaatg
    45001 cgtttcgtca ggccagtaag caccttgccc gggccgactt catagagatg ttctacgcct
    45061 tgcgctgcca tgtactcaac agacttcgtc cactgaaccg ggttatacaa ctgacgtacc
    45121 agtgcgtcac ggatggcatc accattggtt tcgcatttca catcaacgtt attcacaaca
    45181 gaaactgttg gtgcgttaaa ggtgattttc gctaattcta ctgccagttt gtcggctgct
    45241 ggtttcatca gcgcacagtg agacggtacg ctcactggta acggcagagc acgttttgcg
    45301 cccgcagctt tacaggcagc gccagcacgc tcaaccgctt ctttatgacc ggcaataacc
    45361 acctgtcccg gagagttaaa gtttaccgga gaaacgacct gacctgctgc agcttcttca
    45421 cacgctttcg caatagacgc atcatccaga ccgatgattg ccgccatagc gcccgtacct
    45481 tccggtacgg cttcttgcat gagcttgccg cgcatctcaa ccagacgcac cgcatcagcg
    45541 aaatcaatca caccagcgca aaccagcgcg gaatattccc ccaggctgtg accggccatc
    45601 attgccggtg ctttaccgcc ctgctgctgc catacgcggt acagcgcaac agaggcagtc
    45661 aacagcgctg gctgggtttg ccaggtttta ttcagttctt cagctggccc ctgctgggtc
    45721 agcgcccaca ggtcgtagcc cagcgccgca gaagcttcag caaacgtttc ttcgacaatt
    45781 ggatagctcg ccgccatatc agccagcatt ccaacggttt gagaaccctg tccagggaac
    45841 acaaatgcaa attgcgtcat gttttaatcc ttatcctaga aacgaaccag cgcggagccc
    45901 caggtgaatc caccgccaaa ggcttcaagc agaaccaact gccccggctt aatgcgcccg
    45961 tcgcgtacag cttcatccag cgcgcacggg acagaggccg cagaggtatt accgtggcga
    46021 tccagcgtca ccacgacatt gtccatagac ataccgagtt ttttcgccgt tgcactgata
    46081 atacgcaggt tagcctgatg cggaaccagc cagtccagtt gagaacggtc aagattattt
    46141 gccgccagcg tctcatcaac gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg
    46201 ttgcccgcca tcgtcagatg aattgaattc tctggattca cacggtcagc attaggcagc
    46261 gtcagcaact caccatagct accgtcggca tgcagatggg tggagatgat tcccggctct
    46321 tcagaggcag ccagcaccgc agcgcccgcg ccatcgccaa aaataataat agtcccacga
    46381 tcggttggat cgcaggtgcg cgccagtaca tcggaaccga cgaccagagc atacttcacc
    46441 gccccagatt tcacgtattg atcggctacg cttaatgcat aggtgaaacc tgcgcaggct
    46501 gctgcaacgt caaatgccgg gcaaccttta atgcccagca tgctttgaat ctgacaagct
    46561 gcgctcggga aagcgtgcgt agcagaagtc gttgccacaa cgatcaggcc aatctggtct
    46621 ttctcaatgc ccgccatctc aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg
    46681 gtttcgtttg gcgcggcaat gtggcgttcg cggataccgg tacgagtgac aatccactcg
    46741 tcagaggtgt ccaccatttt ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag
    46801 ctgccagtac caataatctt cgtatacatg tacgctcagt cacttttcgg ttatataccg
    46861 tcacttgcaa actgcgagtt cgctggcagc gtcctgctac cgcagagttc cgcttttgcc
    46921 accgtccagc agctcaaaac cagctgggta tacagattcc aggcgagcgg caattcgctg
    46981 aggaacttgt cgctgcaccg cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg
    47041 attggctgca ccatgacttt ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt
    47101 atactggtcg gggttgaggt gactgaatcg cctcgtcagg ctcttttgta gccaacgctt
    47161 taataacagt agccaccacg accgtttttt cccttcaccc tgagatttca gcagagaaag
    47221 gaacatcctg acaacacctt ccatcgtctt taatgtgaca tttcctgtaa agccgtcaca
    47281 aaccagcaca tctgtcttgc cagttaacaa ctcattggct tcaagatagc cgatataatt
    47341 gatagaaggg attgttttaa gcaccgctga ggcatcccga atactgtcga gaccctttac
    47401 ttcttcttca ccaatattga gcaacgccac gcgaggattg ggaatttcca ccacctcttc
    47461 agccagaact gagcccataa tggcaaattg caccaacatt gtactgtcac aatcgacgtt
    47521 ggcccctaag tcaaggacca ccgttttgcc cttttgctga tgtggtaata ccgtcaccag
    47581 cgccggacgc tcaatcccct ccaggggctt gagtaataat tttgccagcc ccatcagtgc
    47641 cccggtatta ccggcactga cacaggcttg cgctcgacct tctttcacca gctccagggc
    47701 cacgcgcatt gaactcccac gactggcgcg gatagcttgc gaaggccggg catcactggc
    47761 gataactgac tgcgcaggaa taatctgcag acgcgaacgt tgttcaaagt cagctttagc
    47821 aagtaatggc gtgatggcgt cgggattgcc gactaaaaga agagtgagtt gcgaattaga
    47881 attcagtgcc tgcaatgctg caggcactgt cacggaaggg ccaaaatccc ctcccatgac
    47941 atctaacgcc agggttagac gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca
    48001 gttgcctggg gaaaatcctc actaagcttc atcacgcaga cgcgtgatta cttagcgatg
    48061 accttgcggc cgcggtagta accgtcggca gtgatgtggt gacgcaggtg tttttcacca
    48121 gaagttttgt ctacagacag gctggtgact gcggtcagcg cgtcatggga acgacgcatg
    48181 ccacgtttgg aacgggttgg tttattctgt tgtacggcca tggaccttac tcctcaatta
    48241 cggtttatcc ccgatccaac ggggagcacc aattacttac gctttaagct ggctaatacg
    48301 gcaaatgggt ttggcttttg cacttcttca ggcagttcac caaagaccat gtccgcttcg
    48361 gacacttcac agtgttcaga atcatgcacc ggaactaccg gcaaggcgag gatgatttca
    48421 tcttcaacca ttgcaagcag atcgatttca ccgaattcgt taacctcaat cggttcatac
    48481 gcttccggca gtgcttcagc ctgttcgtct gaacgcacag gactaaaaca atacgttgtg
    48541 tagacctgat gagtaaacgg cttcccgcaa cgctgacact cgagcgttac cgtcaccttc
    48601 gcatcgccgt ttaacactgc gagacgttgg ttatcgatag cgaacgacat ggagcattcc
    48661 acatcactgt ccacactgac tacggattcg gcgacgcgct caacctgatc aggggtatag
    48721 ataccctggt aatcaaggcg tttttgagcc gtacgaaccg gatcgagagt caggggtaat
    48781 tttacctttt gcatagggcg cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag
    48841 gcagcctgtg gttgcctttt gccaataatt cgcacacatt gcgggttact gctttatttt
    48901 cgttcagtgc tgataacgaa catcaacctg caaaacgaag cgcatagttt aaaatggcta
    48961 tcatcaatac gctatatctg atggaaaaaa atatgcctaa acttatttta gcctccacct
    49021 cgccatggcg tcgcgctctt ctggaaaaac tgcaaatctc ttttgaatgt gcagcaccag
    49081 aggtcgacga aaccccacgc agcgacgaat caccgcgaca gttggtgctt cgactggcac
    49141 aagaaaaagc gcaatctctg gcgtcacgtt atccggatca tttaattatt ggttcagacc
    49201 aggtatgtgt tcttgatggc gaaatcaccg gtaaaccgtt aacggaagaa aatgcctgtc
    49261 tgcaattacg caaagccagc ggcaatatcg tcaccttcta tactggactg gcgttgttta
    49321 attcggcgaa tgggcatcta caaacagaag tggagccttt tgacgtccat ttccgtcacc
    49381 tgagcgaggc ggagattgat aattacgtgc gtaaggagca tcccctgcac tgcgcgggta
    49441 gctttaagag tgaaggattt ggcattacgc tgtttgagcg tttagaggga cgtgacccta
    49501 acacgctggt tggtttaccg ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa
    49561 acccgctgat gggatagttg tttgcgctgg atgcggttac gtcgcatccg gcaatcggtg
    49621 caggcggcct gatgcggcgt aaacgcctta tcaggccaac cgcccattat caggcttata
    49681 tgcttgtatt agcgcgcgtt acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc
    49741 ggcgcttcga tacgcatcac ctcaccggtc cccggatggg taaacttcaa cgctgcggcg
    49801 tgcaggaaca gacgatttaa tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg
    49861 tcaccgtagc gatcgtcaaa ggcaatcgga tgacccgcat actgtgtatg cacacggatc
    49921 tgatgagtac gtccggttac cgggctacaa cgcaccaggg tggcaaatgc atagcgttct
    49981 tccactttaa agcgtgtttc cgacggtttg ccttcctgac tgacacgcac gatacgttcg
    50041 ccgctttgca gaatattttt caataacggc gcttgaacgc tcttcacatg cgactgccac
    50101 tgaccgcgca ccagcgccag gtaatctttt tgcattcctt tttcacgtaa ttgctcatgc
    50161 agagaacgca acgccgagcg ttttttcgct atcagcaaca cacctgaagt gtcccggtcg
    50221 agacgatgaa ccagttcaag gaaccgcgct tccgggcgca acgcccgcaa accttcgata
    50281 acgccgaagc ttaaaccact gccgccatgt accgccgtac cggaaggttt attcagcacc
    50341 aggatatgat catcttcata taagatgacg tccgccagcg ccgccacctt ttgcagatgt
    50401 ggcgaaaccg cctcttcttc ccgctcagca acgcgaaccg gtgggatacg cacctcatca
    50461 cccgcttcga gtttatattc aggcttaata cgttttttgt tcacccgcac ttcgccttta
    50521 cgcaaaatac ggtaaatcat actttttggt acgcctttca attgggtacg caaaaagtta
    50581 tcgatacgtt gccccgcttc gtcagcggtg atagcaacaa tttttacgga tggagtctct
    50641 gttttcatgg tgggcgattc taattagcca acaggattcg cgccactcat ttttctatgc
    50701 ttatatttag ttttgcacct tattacttca ctacgtgatc actttattga tggttattaa
    50761 accaatcacc agcaagaagt gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc
    50821 atcttgctat aacaaggctt gcagtggaat aatgaggccg tttccgtgtc catccttgtt
    50881 aaaacaagaa attttacgga ataacccatt ttgcccgacc gatcatccac gcagcaatgg
    50941 cgtaagacgt attgatcttt caggcagtta gcgggctgcg ggttgcagtc cttaccggta
    51001 gatggaaata tttctggaga gtaataccca gtctgtttct ttgataattg cgctgttttt
    51061 ccgcatgaaa aacgggcaac cgacactctg cgcctctttg agctgacgat aaccgtgagg
    51121 ttggcgacgc gactagacac gaggccatcg gttcacaccc ggaaaggcgt tactttgccc
    51181 gcagcttagt cgtcaatgta agaataatga gtaagttacg atgaaaagaa tgttaatcaa
    51241 cgcaactcag caggaagagt tgcgcgttgc ccttgtagat gggcagcgtc tttatgacct
    51301 ggatatcgaa agtccagggc acgagcagaa aaaggcaaac atctacaaag gtaaaatcac
    51361 ccgcattgaa ccgagtctgg aagctgcttt tgttgattac ggcgctgaac gtcacggttt
    51421 cctcccacta aaagaaattg cccgcgaata tttccctgct aactacagtg ctcatggtcg
    51481 tcccaacatt aaagatgtgt tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga
    51541 agagcgcggc aacaaaggcg cggcattaac cacctttatc agtctggcgg gtagctatct
    51601 ggttctgatg ccaaataacc cgcgcgcagg tggcatttct cgccgtatcg aaggcgatga
    51661 ccgtaccgaa ttaaaagaag cactggcaag ccttgaactg ccggaaggca tggggcttat
    51721 cgtgcgcacc gctggcgtag gcaaatctgc tgaggcgctg caatgggatt taagcttccg
    51781 cctgaaacac tgggaagcca tcaaaaaagc cgctgaaagc cgcccggctc cgttcctgat
    51841 tcatcaggag agcaacgtaa tcgttcgcgc attccgcgat tacttacgtc aggacatcgg
    51901 cgaaatcctt atcgataacc cgaaagtgct cgaactggca cgtcagcata tcgctgcatt
    51961 aggtcgcccg gatttcagca gcaaaatcaa actgtatacc ggcgagatcc cgctgttcag
    52021 ccactaccag attgagtcgc agatcgagtc cgccttccag cgtgaagtcc gtctgccgtc
    52081 cggtggttcg attgttatcg acagcaccga agcgttaacg gccatcgaca tcaactccgc
    52141 acgcgcgacc cgcggcggcg atatcgaaga aaccgcgttt aacaccaacc tcgaagctgc
    52201 cgatgagatt gctcgtcagc tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt
    52261 catcgacatg acgcctgtac gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt
    52321 gcgtcaggac cgtgcgcgta ttcaaatcag ccatatttct cgctttggcc tgctggaaat
    52381 gtcccgccag cgcctgagcc catcactggg tgaatccagc catcacgtct gcccgcgctg
    52441 ctccggtacc ggtaccgtgc gtgacaacga atcgctgtcg ctctctattc tgcgtttgat
    52501 cgaagaagaa gcgctgaaag agaacaccca ggaagttcac gccattgttc ctgtgccaat
    52561 cgcttcttat ctgctgaatg aaaaacgttc tgcggtgaat gcgattgaaa cgcgtcagga
    52621 cggtgttcgc tgcgtgattg tgccaaacga tcagatggaa accccgcact accacgtgtt
    52681 gcgcgtgcgc aaaggggaag aaacgccgac cttaagctac atgctgccga agctgcatga
    52741 agaagcgatg gcgctgccgt ctgaagaaga gttcgctgaa cgtaagcgtc ctgaacaacc
    52801 tgcactggca acctttgcca tgccggatgt gccgccagcg ccaaccccag ctgaacctgc
    52861 cgcgactgtc gtagccccag caccgaaagc tgcaacggca acaccagcag ctcctgcaca
    52921 acctgggctg ttaagccgct tcttcggcgc actgaaagcg ctgttcagcg gtggtgaaga
    52981 agccaaaccg accgagcaac caacaccgaa agcagaagcg aaaccggaac gtcaacagga
    53041 tcgtcgcaaa cctcgtcaga gcaaccgccg tgaccgtaat gagcgccgcg acacccgtag
    53101 tgaacgtact gaaggcagcg ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca
    53161 gcagcagact gccgagacgc gtgagagccg tcagcaggct gaggtaacgg aaaaagcacg
    53221 taccaccgac gagcagcaag cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa
    53281 acgtcaggcg caacaagaag cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac
    53341 cgaacaggaa gaacgtgtac gtccggttca gccgcgtcgt aaacagcgtc agctcaatca
    53401 gaaagtgcgt tacgagcaaa gcgtagccga agaagcggta gtcgcaccgg tggttgaaga
    53461 aactgccgct gccgaaccaa ttgttcagga agctccagct ccacgcacag aactggtgaa
    53521 agtcccgctg ccagtcgtag cgcaaactgc accagaacag caagaagaga acaatgctga
    53581 taaccgtgac aacggtggca tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt
    53641 gagtggtcag cgtcgtcgtc gctatcgtga cgagcgttat ccaacccagt cgccaatgcc
    53701 gttgaccgta gcgtgcgcgt ctccggaact ggcctctggc aaagtctgga tccgctatcc
    53761 aattgtacgt ccgcaagatg tacaagttga agagcagcgc gaacaggaag aagtacaagt
    53821 gcagccgatg gtgactgggg tccctgtcgc cgccgctgtc gaacctgttg ttagcgcacc
    53881 ggttgttgaa gaagtggctg aagttgtaga agcccccgtt cagattgccg aaccgcaacc
    53941 ggaagtggtt gaaacgacgc atcctgaagt gattgccgcc gcagtaactg aacagccgca
    54001 ggtgattacc gagtctgatg ttgccgtagc ccaggaagtt gcagaacacg cagaaccggt
    54061 ggttgaaccg caggaagaga cggcagatat tgaagaagtt gccgaaactg ctgaggttgt
    54121 ggttgctgaa cctgaagttg ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc
    54181 agaagttgaa acggtagctg cggttgaacc tgaggtcacc gttgagcata accacgctac
    54241 ggtgccgatg acgcgcgctc cggcaccgga atatgttccg gatgcaccgc gtcacagtga
    54301 ctggcagcgc cctacttttg ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac
    54361 acatcatgcc tctgccgctc ctgcgcgtcc gcaacctgtt gagtaataat tagctcaagt
    54421 aatcaagccc tggtaactgc cagggctttt ttatttcatc tttgaatcaa catattaaaa
    54481 ttcatcgaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa tgcccgcccc
    54541 ggcgatccca gagcaggcag acaaaacata cccagtttca tgatatgttt taaagcgaaa
    54601 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    54661 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    54721 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaatg aatccagcga
    54781 ctccagttcg ttcagctgcg tgcctaattc cgcgcgaacg gtcagcacat tgttcagcga
    54841 gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc
    54901 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    54961 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    55021 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    55081 ttctgttccg ccgacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    55141 accggcaaaa atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc
    55201 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    55261 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    55321 actctcttcc agtgacactt tttgagtggc gaaagtacgc gccagcgtgt actggctgtt
    55381 ttgcgcctgt gcctgggaga gaacaaccgc ttgtgatgca gcaatgggat catctgaagg
    55441 gttaacgact cgcttacccg tcgacatctg ttcgccgtac ttcatccatt ctgcctgaga
    55501 attggtgata ccacgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    55561 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    55621 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg
    55681 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    55741 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    55801 tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    55861 tgaccgttgc ggttatcgct atcacccgca tctttttcgc tcgccatcgc tattttcgct
    55921 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    55981 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    56041 ccgttggcat ccggcgtcac cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    56101 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    56161 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg
    56221 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttgggtg
    56281 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccagc
    56341 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    56401 atctcaatat tgcctgccgt cccatcaaca taagcgacag tcgtacgaga agggtcagcg
    56461 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    56521 gccaccgtga tgttataagt gccgccatcc tgaacgctga cttctacacc aacaatctgg
    56581 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    56641 cccacgcctg tcaggcgcga gatttgatcg ttcaggctgg caatttgttt agcgtaattg
    56701 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga
    56761 tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    56821 cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    56881 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    56941 tcatagcggg cggtcaggcc gctactttgc gtctgcgcag cacgtaactg gttggtaata
    57001 aacgcatcat actcacgctg cacaccagaa acgtagacgc cattgccaac ccagccgcca
    57061 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    57121 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    57181 ctcatggcgt tattgatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    57241 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    57301 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctggatcatg ttggtgagtt
    57361 tacgggcata gtgaggatcg gtagcatacc ccgcgtcctg tagggcctgc gccccctgct
    57421 ccgcactcgc ggctgtcgtc acggcggcgt agcgcgggtt acgtgttaac agcccaacgt
    57481 aatccgacaa tgcttccaga tacgagctgt agacccgaaa tttcgctttt actttcttcg
    57541 cttcgccatt ttcatattca gtcgtggtga tttcagtaac tggacctttc cagttgccag
    57601 aggctttgac gccaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt
    57661 gcccccaacc agactccagc gccgcctgag cgaggatcaa atgatgtggc acaccgcttt
    57721 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacccg
    57781 gcagcgaatc atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa agcgcctgat
    57841 tttgataacg caccacagtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    57901 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    57961 tgcccgccgt catctgttgg gcaatctgct ggtcatacat actggtatac aggcgagtgt
    58021 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    58081 gcacgaacat cccttccacc tgacgggcca ccggacggat atttgccgcc ggatcttcgc
    58141 ccgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    58201 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    58261 tgattgcagt atggacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac
    58321 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    58381 atcgatctgc gtttgtggag taaccacagt ctgtccacca ccaaacggtg tatctggctg
    58441 gctgacattg gcctgacgat tgactgttac tgagagattt ccctgcgcta ccgcgcagct
    58501 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    58561 agcgtcctgc ggggtgacat taacctgcat attctggata tctgcaagga aacggacctg
    58621 ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    58681 gccatatcca cgcacgcggt tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    58741 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    58801 aataaccgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa cactgctacc
    58861 gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    58921 actgtcaacg cccttaagcg gcgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    58981 tcccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    59041 ctctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    59101 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    59161 ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct
    59221 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    59281 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    59341 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    59401 acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    59461 gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt aatggcaatc
    59521 tgtttttcac ccaccacatg caggttgccg ttgaccagta cctggtcaac cgtcaccgtc
    59581 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    59641 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    59701 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    59761 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    59821 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    59881 ttggcgacgg gcgtcggacc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    59941 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    60001 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct
    60061 gccaccggtt aagccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    60121 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    60181 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    60241 cggcgtgctt tcgttcggta caccagagga ttgcgtttcg gtgtagaggt tttcgccaat
    60301 gctctccagc ccggtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    60361 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    60421 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagctg cccgttctga
    60481 tccacctgga aagagccgtc acgggtatag gctgatgaac catctggcaa catcacctgg
    60541 aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga caggtttccc
    60601 tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg taatccggag
    60661 ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg ataaagcaga
    60721 tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc caggttgttg
    60781 gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat ccataatgaa
    60841 ctgatcataa aatgtcctgt agtggattag tgacaaagtg catatgtctt cttgccggat
    60901 gcggcgtaaa cgccttatcc ggcctacaag tgctcgcaaa atcgataaat tgcgattgcc
    60961 cccatagacc cgataaactt gcgcatcggg caacttatac ttgtcatgta tcctttcaat
    61021 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    61081 ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca
    61141 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    61201 accggccctc gcgtggcctg ggtttctgcg cttaaacgaa aaatgccgtc gtcaccgcgc
    61261 tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc ctactggcgc aactgtattt
    61321 gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatagt gatttccgct
    61381 ccttccggca cagcaattgg ccccgcctcg cctatcacct gatgcccctg aattgtcagt
    61441 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct gccgtcagcg
    61501 gtctgcacgg ccagccagcc atcctgctgc aacgcgacgt ccagcgggcg cgaggtgtaa
    61561 tccattttgc cgggcgtcat atctgcgccc ggcgttgacg ccgtgaccaa cgtgcgcgtg
    61621 ggcagagaaa gcccttccac tggcaccgcg cgtaaagcat tcaactgcgc gcgaaaaccg
    61681 ggcgttgagg cattggccag attactggcg gttaccgcct gttgattcag tgtctggctg
    61741 gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca gcgattagcg
    61801 taagttaacc agcgtgttga ggatctggtc ctgagttttg atggtctggg cgttagactg
    61861 atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga cattggacgc
    61921 ttccaacgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc
    61981 cacgccagaa gattgcgtcg cagaccagac gttgtcgcct tcggatgcca gaccttcgtt
    62041 gttggcaaag ttcgccagta caatctgccc cagcagttgt gtttgttcgt tggaatagtt
    62101 gccgacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc
    62161 attctgggtg gttgccacaa tattgttagc gccggtattt tgctgcatgg agttgaggaa
    62221 gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccagtggtaa tattctctgt
    62281 tggatttgag gtcagaacgc cattggcatt aaacaccagc ttcattgcag gctcggctgt
    62341 acctgttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    62401 aaagtagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    62461 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact
    62521 ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt ttcgctgcca tcagggtatt
    62581 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    62641 cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt catccagctt
    62701 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    62761 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    62821 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    62881 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    62941 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    63001 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    63061 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    63121 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    63181 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gtaccgtccc aggtgaaact
    63241 gtggactccg gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    63301 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    63361 cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagtggtgc cggggatcat
    63421 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    63481 aatagatccg agcgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    63541 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    63601 caccaacaaa gtcagaaaac tgctttgtaa gtctgcggcg ttgctgcccg tgagcgaact
    63661 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    63721 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccatgttg
    63781 agcacttcaa cattggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    63841 acgacatcaa cgttcggcat ttttacgtag ccctttgcat ccgccagcgg attacccggt
    63901 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    63961 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    64021 ccatcgggac cggtcacgct atcagcattc gccagattac tggccgccac gttcaagcgc
    64081 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    64141 taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa cgcgctaagg
    64201 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgacg
    64261 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggtaggaggc
    64321 gtcagcgcct gcgccggaat gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt
    64381 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    64441 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    64501 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct
    64561 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    64621 ggaataaacg caaaatgggt cgctatttat gccgttgatg accattgcgg acaggtacaa
    64681 ttcacgttgt agaaatggct gggggcgaaa atgctggcaa taaaacgtag cgtggcgatc
    64741 atctcgatac tattcactcc gttaagtgcg gcgagtaatc tcacatcgca attgcaaacc
    64801 ttttttagcg cccagctcgc gggggtaagt gatgaggttc gtgtttctat tcgtacagcg
    64861 cccaatctac taccgccgtg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg
    64921 tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat
    64981 gtacaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg aggaaagctg
    65041 gaagctggca atgtcaaact aaaacgcgga cggcaggata ccctgccacc gcgtacggtg
    65101 ctggatatca atcaacttgt tgatgccgtt agcctgcgcg atctatcacc cgatcaacct
    65161 atccagttaa cccagtttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaacgtg
    65221 atcgccagcg gtaacgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc
    65281 gtcacacaga atgcgcgggt gcgcatggta tcgggactgg tggtcagcgg cgttgttgat
    65341 gcagatggga atattcttat aaaccagtaa gctgttaaag attacccgtc ccttgccgat
    65401 aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc
    65461 acttcgcctc tgaagcccgt aagcaccgtt caaccacgcg aaaccactga cgcgtcggta
    65521 acgaacaccc gggcggcaaa aacaacagcc tccaccagca ccagtgtgac gttaagcgac
    65581 gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg tgtcgaagcg
    65641 ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg
    65701 ctgatcaacg aagcgcagca agacttgcag agtaactgat cgtatgacac gtcttgcaga
    65761 gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg atcaagaaca
    65821 gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta cagaacaaaa
    65881 aagctcactg ctggcgacgc tggattacct cgaacagtta cgcaggaaag aacccaatac
    65941 agcaaatagc gttgatatta gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact
    66001 acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc gcaatcaaca
    66061 ggcgctggaa atgttgaaac cgcatcagga accgacgcta tatggggcga acggtcagac
    66121 ctcaacaacc catcgcggcg gtaaaaagat ttcgatctga atattatctc cggcctgcac
    66181 tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca
    66241 gtaccgccag tgcagcgaag tacgcggcaa tccccgccag cacgaccgcc attaaacgca
    66301 gtaagcgcca aggcatggta cccaatgacc actccggcat gatatgtaac atacctaaaa
    66361 gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc atccagccgg
    66421 gttgcggagt aaagattttc tgcttacgca actgccagta aagcagcgaa gcattcagac
    66481 acgccgccag accaatagaa agtgacagcc cggcatgttt caacggacca ataaacgcca
    66541 ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc gttttaatgt
    66601 cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca atcaaaccca
    66661 ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaacgcggta aatttaccgt
    66721 actggaacag cgaaacggtt aacggacccg aaagaatgcc caacgcaacc gcactcggca
    66781 gcgccaacag gaaacaaaga cgcaaccccc agtccatcaa acggttgtat tcatcatgat
    66841 tgccactggc aaaacttttc gacagcgacg gcagcaaaat ggtgccaagc gccacgccca
    66901 gcacaccgga gggaaactcc attaagcggt cggcgtaata catccaagac accgaaccgg
    66961 aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc acagagacgc
    67021 caaggatcgc cggtcccatc tgtttcacca cgcgcattgc tccggcatcg tggaagttaa
    67081 tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg tagctgatac accagctgca
    67141 ggatgccgcc gaccgtaaca gcccacgcca gcgccagcac cggtgggtta aagtacggtg
    67201 cggcaaacag cgcgaaacca atcatgctga tgttaagcag tgttggcgca aacgccggaa
    67261 tcgagaagcg gttccacgta ttcagaatcg ctcccaccag cgacgccagg gagatcagca
    67321 agatataggg aaaggtaatc tttagtagct ggctggtcag ggcaaatttg tcagctgtgt
    67381 cagcgaagcc tggcgcggtc accatgatca cccacggtgc ggcgagcatg ccagcgaccg
    67441 tcacaaccgc cagcgcaagt gtcagcaggc cagaaacata agagacaaag acccgcgtgg
    67501 cgtcttcacc ctgcttactt ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg
    67561 ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt
    67621 cggttgccat ccctgcgcca aagattctgg cgacaattgc gtctcgtgcg aagccaagca
    67681 cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa ttcatcggtg
    67741 ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt
    67801 ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc actcatcgca
    67861 tcgcgccaga tcttgtcaac gatacgttgc gccagcactg cctgttcgcc ggcggtttgc
    67921 agaactgtct gattttgcac acattcaatg aagtgccgcg cacagccgac aaatccacgt
    67981 tgctcaagag tgctctgcca accaggaatc ggtttatgca ctacgccctg cccgcgctcc
    68041 tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg
    68101 gtttcacgct gacttccggc acggcgatgc atacaggtgg tgatctgcaa aggatcggcc
    68161 gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca
    68221 tccagagagg ctttgccgcc cgacaaccac agcgcggtat ccaccacatg cagataatca
    68281 tccagcaacg tgaaataaag atcgtgtggc ccgacactat tgctacgatg tttatccatt
    68341 cttagcgaag ctgcggtagc gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga
    68401 cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac cagccgttca
    68461 gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacacc cgcattgagt
    68521 aacgtactga ccacgtcaaa gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa
    68581 ctggcggcaa ggctggataa cgaatcggca taaggaatgc gccagctttc acaaattggc
    68641 agggctttcg cgcgcgtagg cgaccaggct ccttgtaaca tccagtcaga cgctgccgcc
    68701 agcaccggta accacgcttt ttgcgcaatg ccgcctaatc ccacaacgcc gatacgtaat
    68761 tttttcactt taatctccca gatgggccag caacgaatca agacgctgtt tcagttctgc
    68821 cacttcgatt tccagggctt cgacgcgggc ctgtaaatca ccatcaaccg cgttcgacat
    68881 atccgttacc gccggctgat cctctacctc accactgaaa agatgcatgt agcggctttc
    68941 gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc gatttgccag
    69001 ttgttccagc gtcgactcca cttccgccat atcgctgaat tcatacattc gcgcggcgcg
    69061 gctgcgcagt tcacccggcg tctgggcacc acgcaataac aacgtggtga ttaacgccac
    69121 ttccgctgcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt gttcatattt
    69181 ggtgacccga ttaccaaagc cactcaccgt gcgcagataa tggcgtttga ccagattatc
    69241 cagttgctcc tgcacttcag attcactcag attcatgacc ggttcacggt tcgttttctg
    69301 attacaggcc gtgactacac cattgactga gagcgggtat tgctccggcg tggtcacctg
    69361 cttttccagc aagcaaccaa tcacgcgagc ttcaagggca gtaagttgat atttcatgag
    69421 attctcctta gcggccgggc gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc
    69481 gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca aaacccagtc
    69541 gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata atgcgatgaa
    69601 tatgttgggt gcgctgcata taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt
    69661 tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa gagccacgaa
    69721 caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg aataagccaa
    69781 agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccccagcctg gcctgccagc
    69841 ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg aaatggcgat
    69901 tctctgcgta ataatccgca agacgccagg catcacgatc atgcaccaga cgcacgacca
    69961 gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac attctatacc
    70021 tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc gcaaccagac
    70081 cctgtgaaaa gtccaaaatg ccattttctg cattattcac atatttgata aaaactctct
    70141 atcaaagcga cgttttgata aaaaaatatt gtccccgaag tgatgggaaa agtcttagcg
    70201 gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg tcccgcgtat
    70261 cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg gtcgtgctgg
    70321 ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg
    70381 ccgtcatggt cggtattgct ctcggtctac gccagtttat tcagcaaggt ctgggtattt
    70441 tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgttacc ggtatgctga
    70501 tgcgcgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta ttgtggtttt
    70561 catgcctgct ctcgggactc ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg
    70621 tgaaactaat ccgtccacaa caacgttgtc gttttttctc gctgttgatg atgcaggaca
    70681 gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac gactttcgcc
    70741 tggtctgcgc cacaggggcc gttttgtttg tgctgtgtgc ggcgttcaat gcgtggttgt
    70801 taccggcatg gaaactctcc accgtacgca cgcccgttcg cgaaggcatg acccgcgtga
    70861 tgcgtgacaa gcgttttgtc acctatgtcc tgacgctggc gggttactac atgctggctg
    70921 tacaagtgat gctgatgctg ccaattatgg tcaacgacgt ggctggcgcg ccctctgccg
    70981 ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc
    71041 gctggagtga aaagcatttt cgtctggaac accggttgat ggctgggctg ttgataatgt
    71101 cattaagcat gatgccggtg ggcatggtca gcggtctgca acaacttttc accctgattt
    71161 gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta agtgctttgc
    71221 tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt ctggcgattg
    71281 gcggcgctat tggttatatc ggtggtggct ggctgtttga cctgggcaaa tcggcgcacc
    71341 agccagagct tccgtggatg atgctgggaa ttattggcat cttcactttc cttgcgctgg
    71401 gttggcagtt tagtcagaaa cgcgcaacgc gtcgtttgct ggaacgcgac gcctgatttg
    71461 atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg aggagtcacg
    71521 tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg attttcggcc
    71581 tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa acacccaccc
    71641 ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc tatatgccag
    71701 aaagcatgga tatcgttcac tatgtcgata aactcgacgg caaaccgtta ctgaccggca
    71761 aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa tggctacgcc aacaaactgc
    71821 tgttgccgcg ttttgccaaa tcggcatttg atgagttttc tactcccgcc gcgcgcaaat
    71881 atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg gcccactctg
    71941 acggtctgat taagaatatc agcgatgatt tgcgtgcgct ggacaaactg atcgtcaaac
    72001 cgaacgccgt gaatggcgaa ctttcggaag atgatattca gctatttccg ctactgcgta
    72061 atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac cgcgataata
    72121 tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc tgacccgccg
    72181 cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc gagtttatcg
    72241 ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc gaggactacc
    72301 atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat
    72361 caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc gaaacataat
    72421 aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatattgt tctgacaaac
    72481 ttgatcagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg agacgccaat
    72541 ctggacatga actccctgtt cggtagtcag aaagcgacca tgaaactgaa gctgaaagcg
    72601 ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga aagagatgga agtggtcgat
    72661 gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac
    72721 caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga agatggcagt
    72781 cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt
    72841 gtcattccgt ttacggatta atgacgaggg cgcattcgcg ccctttattt ttcgtgcaaa
    72901 ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca ttccttcagc cggagcatag
    72961 agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga ctggcacctt
    73021 cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga aatttatgga
    73081 cgggctatcg taatgcccaa tctggctccg cccgtgacca cagttgaggc tgccgtggcg
    73141 tatcgccaac gtattcttga cgccgtacct gccgggcacg atttcacccc attgataacc
    73201 tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa tgaaggcgtg
    73261 ttcaccgctg caaaacttta cccggcaaac gcaaccacta actccagcca cggcgtgacg
    73321 tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat gccgctactg
    73381 gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc gcgctttata
    73441 gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc tgaaagtcgt ttttgagcac
    73501 atcaccacca aagatgctgc cgactatgtc cgtgacggaa atgaacggct ggctgccacc
    73561 atcactccgc agcatctgat gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg
    73621 cacctgtatt gtttacccat cctcaaacgc aatattcacc aacaggcatt gcgtgaactg
    73681 gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca tgcacgtcat
    73741 cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg ccccaaccgc gctgggcagt
    73801 tacgctaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt ctgttctgta
    73861 aacggcccgc agttctatgg gttgccggtc aacgacacat tcatcgaact ggtacgtgaa
    73921 gagcaacagg ttgctgaaag catcgcactg actgatgaca ccctggtgcc attcctcgcc
    73981 ggggaaacgg tacgctggtc cgttaaacaa taaaaatcgt agcttcctgt tgtcattagg
    74041 ttatttaacc tgtataaata accagtatat tcaacagggg gctattatgc gaattgaagt
    74101 caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg ctggcgaact
    74161 ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg tacgttatgc
    74221 cgcagcgaat aatttatcgg ttattggcgc aacaaaagaa gataaacagc gcattagcga
    74281 aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat aatatgcagt
    74341 gatttttgtt tttgccgggt cgccccggct tttttatttt acttacgaat tttgagtaca
    74401 aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg aatgacatgc
    74461 tgttttattg ttcatataaa tcttaaaagc agaaatttat ctgttccccc ctttaaaaat
    74521 tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa cgcattgaac
    74581 ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa aataatgaag
    74641 tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc tatgatgcgc
    74701 tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg actgaagttg
    74761 gtcaaacgct ctggttaacc actgatgttg ccagacaatt tatttcgata ttagaagcag
    74821 gaatcgccaa aattgaatcc ggtgatttcc aggtaaacga gtatcggcgt cattaacatg
    74881 acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg ggttgtatat
    74941 ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaaaaaa atgaaatacg
    75001 atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat gcaacccgcg
    75061 ccggtttaaa cgtgctaatg accgacgccc atatgccacc gcatcaacac ggcagccacc
    75121 acggcgatac gcgattaatt cgccatgctt atggtgaagg cgaaaagtat gtcccgctgg
    75181 tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca caacgaagat gatcccattt
    75241 ttgtacgctc tggtgtcatt aacctcggcc cggctgactc cgcatttctc gccaacgtcg
    75301 cccacagcgc cgaacagtgg caactcaacg ttgaaaagct cgatgcgcaa gggattatgg
    75361 cccgctggcc agaaatacgc gtcccggaca actacatcgg cttatttgag actgattccg
    75421 gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag gaagcgggct
    75481 gtgcgcaact gttcaactgc ccggtcaccg caattcgtca tgacgatgat ggcgtaacta
    75541 ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg
    75601 taaaagacct gctcccggag cagcctgtcc agcccgtacg taaagtattt gcctggtatc
    75661 aggccgatgg ccgctatagc gtgaagaata aattcccggc gtttaccggt gaactgccca
    75721 atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt ggcaaacata
    75781 acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa gtggtcagcg
    75841 atggttcgga agccttcccg ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt
    75901 acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc gataccctac
    75961 ccggccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt
    76021 cagttttagg ggaaatagct gccgattttg cgcaagacaa aaaaagcgat tttgatttga
    76081 cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa tgaggccgtt
    76141 tttacaggag tatttaatgc gtcggctgtt gcactatctc a
//
LOCUS       DAZRVY010000003        74589 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZRVY010000003 DAZRVY010000000
VERSION     DAZRVY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.003"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHXOX_000165"
     CDS             <1..1015
                     /locus_tag="ACHXOX_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7003752.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXOX_000166"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXOX_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR7003753.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHXOX_000167"
     CDS             1797..1904
                     /locus_tag="ACHXOX_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR7003754.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXOX_000168"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXOX_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR7003755.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXOX_000169"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXOX_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR7003756.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHXOX_000170"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHXOX_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR7003757.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHXOX_000171"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHXOX_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR7003758.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHXOX_000172"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHXOX_000172"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR7003759.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHXOX_000173"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHXOX_000173"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR7003760.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHXOX_000174"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHXOX_000174"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR7003761.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHXOX_000175"
     CDS             complement(11226..11813)
                     /locus_tag="ACHXOX_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR7003762.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHXOX_000176"
     CDS             11983..12561
                     /locus_tag="ACHXOX_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7003763.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHXOX_000177"
     CDS             complement(12691..13452)
                     /locus_tag="ACHXOX_000177"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7003764.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHXOX_000178"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHXOX_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR7003765.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHXOX_000179"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHXOX_000179"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR7003766.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="ACHXOX_000180"
     CDS             complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="ACHXOX_000180"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR7003767.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16807..17205
                     /locus_tag="ACHXOX_000181"
     CDS             16807..17205
                     /locus_tag="ACHXOX_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR7003768.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17202..17897
                     /locus_tag="ACHXOX_000182"
     CDS             17202..17897
                     /locus_tag="ACHXOX_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR7003769.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18027..18911
                     /gene="cdd"
                     /locus_tag="ACHXOX_000183"
     CDS             18027..18911
                     /gene="cdd"
                     /locus_tag="ACHXOX_000183"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR7003770.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19061..19780
                     /gene="sanA"
                     /locus_tag="ACHXOX_000184"
     CDS             19061..19780
                     /gene="sanA"
                     /locus_tag="ACHXOX_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR7003771.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19783..20022
                     /locus_tag="ACHXOX_000185"
     CDS             19783..20022
                     /locus_tag="ACHXOX_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR7003772.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20216..21454
                     /gene="preT"
                     /locus_tag="ACHXOX_000186"
                     /pseudo
     CDS             20216..21454
                     /gene="preT"
                     /locus_tag="ACHXOX_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21448..22683
                     /gene="preA"
                     /locus_tag="ACHXOX_000187"
     CDS             21448..22683
                     /gene="preA"
                     /locus_tag="ACHXOX_000187"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR7003773.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="ACHXOX_000188"
     CDS             complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="ACHXOX_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR7003774.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="ACHXOX_000189"
     CDS             complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="ACHXOX_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR7003775.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="ACHXOX_000190"
     CDS             complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="ACHXOX_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR7003776.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26695..27693)
                     /gene="galS"
                     /locus_tag="ACHXOX_000191"
     CDS             complement(26695..27693)
                     /gene="galS"
                     /locus_tag="ACHXOX_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR7003777.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(27877..29034)
                     /gene="yeiB"
                     /locus_tag="ACHXOX_000192"
     CDS             complement(27877..29034)
                     /gene="yeiB"
                     /locus_tag="ACHXOX_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR7003778.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29051..29719)
                     /gene="folE"
                     /locus_tag="ACHXOX_000193"
     CDS             complement(29051..29719)
                     /gene="folE"
                     /locus_tag="ACHXOX_000193"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR7003779.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29977..30813
                     /gene="yeiG"
                     /locus_tag="ACHXOX_000194"
     CDS             29977..30813
                     /gene="yeiG"
                     /locus_tag="ACHXOX_000194"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR7003780.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="ACHXOX_000195"
     CDS             complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="ACHXOX_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR7003781.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="ACHXOX_000196"
     CDS             complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="ACHXOX_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR7003782.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34802..35683)
                     /gene="yieE"
                     /locus_tag="ACHXOX_000197"
     CDS             complement(34802..35683)
                     /gene="yieE"
                     /locus_tag="ACHXOX_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR7003783.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35782..36831
                     /locus_tag="ACHXOX_000198"
     CDS             35782..36831
                     /locus_tag="ACHXOX_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR7003784.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36905..37762
                     /gene="nfo"
                     /locus_tag="ACHXOX_000199"
     CDS             36905..37762
                     /gene="nfo"
                     /locus_tag="ACHXOX_000199"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR7003785.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37766..38854
                     /locus_tag="ACHXOX_000200"
     CDS             37766..38854
                     /locus_tag="ACHXOX_000200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR7003786.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38910..40160)
                     /locus_tag="ACHXOX_000201"
     CDS             complement(38910..40160)
                     /locus_tag="ACHXOX_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR7003787.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="ACHXOX_000202"
     CDS             complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="ACHXOX_000202"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR7003788.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41331..42029
                     /locus_tag="ACHXOX_000203"
     CDS             41331..42029
                     /locus_tag="ACHXOX_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR7003789.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42096..43346)
                     /locus_tag="ACHXOX_000204"
     CDS             complement(42096..43346)
                     /locus_tag="ACHXOX_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR7003790.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="ACHXOX_000205"
     CDS             complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="ACHXOX_000205"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR7003791.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44366..45307)
                     /locus_tag="ACHXOX_000206"
     CDS             complement(44366..45307)
                     /locus_tag="ACHXOX_000206"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR7003792.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="ACHXOX_000207"
     CDS             complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="ACHXOX_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR7003793.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="ACHXOX_000208"
     CDS             complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="ACHXOX_000208"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR7003794.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="ACHXOX_000209"
     CDS             complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="ACHXOX_000209"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR7003795.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49909..51090
                     /gene="setB"
                     /locus_tag="ACHXOX_000210"
     CDS             49909..51090
                     /gene="setB"
                     /locus_tag="ACHXOX_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR7003796.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51087..51341)
                     /locus_tag="ACHXOX_000211"
     CDS             complement(51087..51341)
                     /locus_tag="ACHXOX_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR7003797.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51496..52068
                     /gene="yeiP"
                     /locus_tag="ACHXOX_000212"
     CDS             51496..52068
                     /gene="yeiP"
                     /locus_tag="ACHXOX_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR7003798.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52291..53757
                     /locus_tag="ACHXOX_000213"
     CDS             52291..53757
                     /locus_tag="ACHXOX_000213"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR7003799.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53875..54861
                     /gene="yeiR"
                     /locus_tag="ACHXOX_000214"
     CDS             53875..54861
                     /gene="yeiR"
                     /locus_tag="ACHXOX_000214"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR7003800.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDCHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54900..55613
                     /gene="lpxT"
                     /locus_tag="ACHXOX_000215"
     CDS             54900..55613
                     /gene="lpxT"
                     /locus_tag="ACHXOX_000215"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR7003801.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56025..56591
                     /gene="mepS"
                     /locus_tag="ACHXOX_000216"
     CDS             56025..56591
                     /gene="mepS"
                     /locus_tag="ACHXOX_000216"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR7003802.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56772..58328
                     /locus_tag="ACHXOX_000217"
     CDS             56772..58328
                     /locus_tag="ACHXOX_000217"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR7003803.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58410..60224
                     /locus_tag="ACHXOX_000218"
     CDS             58410..60224
                     /locus_tag="ACHXOX_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR7003804.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60225..61319
                     /locus_tag="ACHXOX_000219"
     CDS             60225..61319
                     /locus_tag="ACHXOX_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR7003805.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61319..62344
                     /locus_tag="ACHXOX_000220"
     CDS             61319..62344
                     /locus_tag="ACHXOX_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7003806.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62346..63935
                     /gene="yejF"
                     /locus_tag="ACHXOX_000221"
     CDS             62346..63935
                     /gene="yejF"
                     /locus_tag="ACHXOX_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR7003807.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63939..64283)
                     /locus_tag="ACHXOX_000222"
     CDS             complement(63939..64283)
                     /locus_tag="ACHXOX_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR7003808.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64616..65673)
                     /locus_tag="ACHXOX_000223"
                     /pseudo
     CDS             complement(64616..65673)
                     /locus_tag="ACHXOX_000223"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="ACHXOX_000224"
     CDS             complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="ACHXOX_000224"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR7003809.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66609..68369
                     /locus_tag="ACHXOX_000225"
     CDS             66609..68369
                     /locus_tag="ACHXOX_000225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR7003810.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68494..68778
                     /gene="rplY"
                     /locus_tag="ACHXOX_000226"
     CDS             68494..68778
                     /gene="rplY"
                     /locus_tag="ACHXOX_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR7003811.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="ACHXOX_000227"
     CDS             complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="ACHXOX_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR7003812.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70106..70333
                     /locus_tag="ACHXOX_000228"
     CDS             70106..70333
                     /locus_tag="ACHXOX_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR7003813.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70353..72113
                     /gene="yejM"
                     /locus_tag="ACHXOX_000229"
     CDS             70353..72113
                     /gene="yejM"
                     /locus_tag="ACHXOX_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR7003814.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72188..72264
                     /locus_tag="ACHXOX_000230"
     tRNA            72188..72264
                     /locus_tag="ACHXOX_000230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72222..72224,aa:Pro,seq:ggg)
     gene            complement(72367..74589)
                     /gene="yejO"
                     /locus_tag="ACHXOX_000231"
                     /pseudo
     CDS             complement(72367..74589)
                     /gene="yejO"
                     /locus_tag="ACHXOX_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18315 a        19660 c        18301 g        18313 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgct ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgca cccccgggct ctggcgaaca aaatcgccgc
    15421 tgcgcggtgc cctcggctta tcccttacgg ctaccgggtc gggcgcgagg taacatccct
    15481 gtaaaacgcg ccctcagccc acatccatgt gggctgcccc ggccttcagg gaacgcctcg
    15541 gcaattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa cacattgtca
    15601 aaaaacatca ctatggtttt ttagagtttc tcgatatcaa ttgcctgaat agcccttgca
    15661 atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc ttcatcgtat
    15721 tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa cccggtatcg
    15781 ccctgctttt ccagacgggt atatttttgc agcaaagcaa ccacctccgg ccacggcatt
    15841 cgcggttcgt tatattttac tacccggctc aggttgggaa tattgagcgc cccgcgacca
    15901 atcatcactg cgtcgcagcc gctgatcgca atgcattgtt gcgcgctctg ccagtcccag
    15961 atttcgccgt tggcaatcac cggaatattc agccgctggc gaatctcgcc aatcgcctgc
    16021 cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gtccatgcac caccagctcc
    16081 gtagcaccag cctgttgaac cgcatcggcg atttcaaatt tcttctcacc gctgtcccag
    16141 cccagacgca ctttcacgct gacgggcaaa tgcgccggta cagcttcacg catcgctttt
    16201 gcaccctggt agatgagttc aggatctttg agtaacgtcg ccccgccgcc gctgccgtta
    16261 accgctttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa ttccaccgca
    16321 cgggcggcgt tctctgccag ccattgtggg aactgaccta acaactgaac gcgcaccagc
    16381 gtaccggatg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg aaagactttt
    16441 accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc gtagtcgtta
    16501 acttcggtca gcaattctcg caccagagag tcaagcacac cctccatcgg agccagtaac
    16561 acacgcatat cttcacccgg aaaaaaatga ggcgctatgt tagcgcctat ggtcaacgga
    16621 ttaaaggtct gcaaagaggg attgctgcaa actgtaatcc ctctctatgc ttgatgtatg
    16681 cctgagttcg ttgcgcattg cgtaatgccg gggagcgaaa aaataagcgc tgatttctta
    16741 atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg aatccatctc atctggggtg
    16801 ttgattatga gtaagacact gaacattatc tggcaatatt tacgcgcttt cgtcctgatt
    16861 tatgcctgcc tgtatgcagg cattttcatt gcttccctgc taccggtaac cattccgggc
    16921 agcatcatcg ggatgctgat cctgtttgtc ctgctggcct tgcaaattct tccggcaaaa
    16981 tgggtcaatc cggggtgcta cgtactgatt cgctatatgg cgctattgtt tgtgccgatt
    17041 ggcgtaggcg tcatgcaata ttttgatttg ctccgcgcac agtttggccc ggtagtggtt
    17101 tcctgtgcag tcagtacgct ggtggttttt ctggtggtga gctggagttc acaactggta
    17161 cacggtgaac gtaaagtcgt aggtcagaaa ggatcagaag aatgatggcg aatatctggt
    17221 ggtcattacc gctgacgttg attgtctttt ttgccgcccg caaactggcg gcacggtata
    17281 agtttcccct gcttaacccg ttgctggtag caatggtggt gatcattcct tttttaatgc
    17341 tgaccggcat ctcttacgac agctacttta aaggtagcga agtgctaaac gatctgctgc
    17401 aaccggcggt cgtcgcgctg gcctatcctt tatatgagca gctacaccag atccgcgcgc
    17461 gctggaaatc gatcatcacc atctgtttta tcggcagcgt ggttgcgatg gtgacgggga
    17521 cttccgtggc attgttgatg ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat
    17581 cagtcaccac gcctattgca atggcggttg gcggcagtat tggcggtatt ccggcaatca
    17641 gcgcagtttg cgtgattttc gtcggcatcc tcggcgcggt atttggccat acattgctta
    17701 atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc aatggggaca gcctcgcacg
    17761 ccctcggtac ggcgcgctgc gccgagctgg attatcagga aggtgcattt agttcgctgg
    17821 cgctggtgtt atgcgggata attacttcgc tgatcgcacc gttccttttc ccgattattc
    17881 tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt
    17941 tgcataatta atgagattca gatcacatat aaagccataa cgggttcgta aactgttatc
    18001 ccattacatg attatgaggc aacgccatgc atccacgttt tcaaaccgct tttgcccaac
    18061 ttgcggataa cttgcaatct gcactggaac ctattctggc agacaagtac ttccccgctt
    18121 tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac ggggctggac gaagacgcgc
    18181 tggcattcgc actacttcca ctggcggcgg cctgtgcgcg tacgccattg tcgaatttta
    18241 atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta tttcggtgcc aatatggaat
    18301 ttattggtgc aacaatgcag caaaccgttc atgccgaaca aagcgcgatc agccacgcct
    18361 ggttgagtgg tgaaaaagcg cttgcagcca tcaccgttaa ctatacgcct tgtggtcact
    18421 gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct gcgtattcat ctgccgggcc
    18481 gcgaggcaca cgcgctgcgt gactatctgc cggatgcctt tgggccgaaa gatctggaga
    18541 ttaaaacgct gctgatggac gaacaggatc acggctatgc gctgacgggt gatgcgcttt
    18601 ctcaggcagc gattgcggcg gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg
    18661 gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg cagctacgct gaaaacgccg
    18721 cattcaaccc gactctgcca ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt
    18781 atgattaccc ggatatccag cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc
    18841 agtgggatgc cacctccgca acgctgaaag ctctcggctg tcacagtatc gaccgcgtgc
    18901 ttctcgctta agcctggtgc cggatgcggc gtgaacgcct tgtccggctt gccagccctc
    18961 tcctggtgtc gaaattcccg gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa
    19021 agtagcctga tggaaatttt ccttagatcg agtctcctgc atgttaaagc gcgtgttcct
    19081 cagcctgtta gtcctgatcg gcttgctgct gttgactgtg ctcggcctcg atcgctggat
    19141 gagctggaaa accgcgcctt atatctacga cgaattgcag gatctcccct accgccaggt
    19201 cggcgtggtg ctcggcacag caaaatatta tcgtactggc gtaattaatc agtattatcg
    19261 ctaccgcatt caaggagcga ttaatgccta taacagcggt aaggtaaatt atctattact
    19321 gagcggcgat aacgcattgc aaagttataa tgagccgatg accatgcgca aagatttaat
    19381 cgctgctggt gtcgacccat cagatattgt tctcgattac gcaggctttc gtacgctgga
    19441 ttccatcgtg cgtacacgca aagtgttcga tactaatgat ttcattatta tcacccaacg
    19501 tttccactgt gagcgggcat tatttattgc gctgcatatg gggattcagg ctcagtgtta
    19561 tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt
    19621 cggtgcgctg gctgaccttt atatttttag acgtgaaccg cgttttttag ggccgctggt
    19681 ccctattccg gctatgcacc aggtgccgga agatgcgcag gggtatcccg ccgtcacacc
    19741 cgaacagtta cttgaattac aaaagaaaca aggaaagtag ttatggatgt acagcagttt
    19801 tttgtcgttg ccgttttttt ccttatcccg atattttgtt tccgcgaagc atggaaaggc
    19861 tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg
    19921 cgagcaaaaa atcccggact ctttttcgct tatatggtgg catatatcgg cttcggaatt
    19981 ttatctatcg gcatgattgt ttatcttatt ttctatcgtt aatattttct cttttctgtt
    20041 aattattgag aataattatt acttcactca ataagctgcg gatataacat tctgaaccgc
    20101 agctatttgt gaatcctttc acagtttaaa tttccccaca cgcttagcct taatatcagc
    20161 acgcaatatt tatttagcgt tcgccttaat tactcatagc attaaggaag atcacatgcc
    20221 gcaacaaaat tatctggatg aactcactcc ggcttttacg cctttactgg cgattaaaga
    20281 agcctctcgc tgtttattat gtcatgacgc tccctgtagt caggcttgcc cggcacagac
    20341 cgatccgggg aaatttattc gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac
    20401 aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt
    20461 atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg gccgcttaca
    20521 gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc ccggtactaa
    20581 aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg ccagtgtgac
    20641 actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac ccggtggctg
    20701 attgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg cagagatcgc
    20761 ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta acacactcac
    20821 ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt tatcaagcgg
    20881 ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact tcttgcaacg
    20941 tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta tcggcggcgg
    21001 tgatgtcgcg atggacgtag ccagcacgct gaaagttctc ggctgtcagg cggtaacttg
    21061 cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta ccagcgcccg
    21121 ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag gcaataaagt
    21181 cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata aaattattct
    21241 cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc agcgtaacac
    21301 catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg gcgatattgt
    21361 tgagggagac aaaaccgtgg tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat
    21421 tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat tactttttgc
    21481 ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa ctgctatgag
    21541 atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac gatcggcttt
    21601 tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga taccggtttt
    21661 atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa tctggccgcc
    21721 ctgcgtcggc tgaaagaaga ttacccggac aaagtattga tcgcttcgat catgggggaa
    21781 aatgagcagc aatgggagga gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc
    21841 gagtgtaact tctcctgtcc gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa
    21901 agcccggagc tggtagaaaa atattgtcgg gcagtgaaac ggggttccac gctgccaatg
    21961 ctggcgaaga tgacgccgaa tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc
    22021 ggcggcgcag atggcattgc ggcgattaac accgttaaat ccatcaccaa tatcgatctt
    22081 aatcagaaaa tcggtatgcc gatcgttaac ggaaaatcaa gtatttccgg atattccggt
    22141 aaagcggtaa aaccgatcgc cctgcgcttc attcagcaaa tgcgcaccca tccagaactg
    22201 cgcgatttcc caatcagcgg tatcggcggc attgaaacct gggaggatgc ggcggagttt
    22261 ttattgctcg gcgcagcaac gttacaggtc accaccggca ttatgcagta cggctatcgc
    22321 atcgtagaag atatggcgag cgggttatcg cattatctcg ccgatcaggg atttgattcg
    22381 ttgcaggaga tggtaggtct ggcgaataac aatattgtcc cggcggaaga tttagaccgc
    22441 agttatattg tctatccccg tatcaatctt gacaaatgtg ttggctgtgg acgctgttat
    22501 atttcctgtt acgacggcgg tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg
    22561 cattgtaata ccgagaaatg tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt
    22621 tgtattgagc tcggggaagt gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg
    22681 tagaacccgc gtcacatccg gcatttggtt cctgttgccg gatgcggcgt gaacgcctta
    22741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    22801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    22861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    22921 gttgatgacg gtaaaaataa tcaccccggt caccacgcca atcaccgtcc ccacaccgcc
    22981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    23041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    23101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    23161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    23221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    23281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    23341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    23401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    23461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    23521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    23581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    23641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    23701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    23761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    23821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    23881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    23941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    24001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    24061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    24121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    24181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    24241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    24301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt
    24361 ggcattatgg ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg
    24421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc
    24481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    24541 cgttaggtta cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg
    24601 ctgattaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    24661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    24721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    24781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    24841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    24901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat
    24961 cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    25021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    25081 catcgaaata ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg
    25141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    25201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga attttaaatt
    25261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    25321 gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    25381 aataagggcg cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    25441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    25501 ggtgccatca gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt
    25561 cgcctggttg ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc
    25621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    25681 cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    25741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    25801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    25861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    25921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    25981 caaatcgccc tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    26041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    26101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    26161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    26221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    26281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    26341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    26401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    26461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac ttttttaaat
    26521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat
    26581 tacctcggtg aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    26641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    26701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgcgttgcga cagaatggcg
    26761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    26821 ctgcaaggcc agttcggtgg ctaatttagc cattgaagca atgggataac gcacggtcgt
    26881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    26941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    27001 gttataagca aatatagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    27061 ctcaccgccc ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc
    27121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    27181 actggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    27241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    27301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    27361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    27421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    27481 agcgactaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    27541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    27601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    27661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctaac gcgctacatc
    27721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    27781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    27841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    27901 cgttccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    27961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    28021 cagcagctcc aggcggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcatggtaca
    28081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    28141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    28201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    28261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    28321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    28381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    28441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    28501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggtgcg
    28561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    28621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    28681 gcagattaag cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc
    28741 gtgaataaaa cccagcaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    28801 gggcagcaac atttgcaggc ccgcaccaaa cagcagcgca aaaagcgtga ggaatttcac
    28861 ctggccgatg agatcgagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcgccgta
    28921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    28981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtt acgttgcgct ccatggttcc
    29041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    29101 tggatttgaa caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc
    29161 cacgcgcttt cacgcagtaa tgcaccgcat caatggagac agccacgtta ttggtgccca
    29221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    29281 gggcaaagaa ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga
    29341 tataagccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca
    29401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    29461 ttttcgggaa gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    29521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    29581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    29641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    29701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    29761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    29821 tccagtattc acagcgataa ttattgtaat tgccgctgac tttcatcagc agatgttaaa
    29881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    29941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    30001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    30061 acgttcagta tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg
    30121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg
    30181 gcggcagaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    30241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    30301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    30361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    30421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    30481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cacctattta
    30541 ggtgaggaca aaaatgcatg gctggaatgg gatagttgcg cgctgatgta tgccagtaac
    30601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    30661 cagttgcaac ctgcggtatt ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    30721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    30781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    30841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    30901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    30961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    31021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt
    31081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    31141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    31201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    31261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    31321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    31381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    31441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    31501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    31561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    31621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    31681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    31741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    31801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    31861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    31921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    31981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    32041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    32101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    32161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    32221 tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta aagaactgac
    32281 cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac
    32341 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    32401 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    32461 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttcacgcgtt
    32521 taccgtcgac gagaatcagg gtatagctgc tgtccagacc acgaatacta acgcccttac
    32581 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    32641 tctgtaccgg ttttcgctgc agatcttcct gggtaatgac gctgatactg gcgggtgcat
    32701 ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga
    32761 ccgctaacac aggccataca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    32821 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggtatcgc
    32881 tcacatcttc tttacgtccc cttgcgtacg gcagcatcgc ggtggactta tttgtatgca
    32941 ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt gattgtagcg
    33001 taacgataat gcaaacgata acagttatca atccaaatgt taaattttat atccgcatga
    33061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    33121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    33181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    33241 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    33301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    33361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    33421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    33481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacaaaccg gcaatcaccg
    33541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga caatttagcg aaaatgcgcg
    33601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    33661 tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    33721 gacccgcgtg ctggaacacc aaggtgaacg gactgacgct gatgtcttta acatcgttac
    33781 gcagcaggct cggatcggta tacggaataa tcaggctgat aatcaggatc gcgaacacat
    33841 agaacaacag gattcgccag aatacctgac gtaccgcgcg tggaatgttt ttcgccggat
    33901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    33961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    34021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    34081 cgatgataaa gacaataact gtcgtgactt tgatcagtga aaaccagtat tccgcttcac
    34141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    34201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgtgct gcaaccaagt
    34261 caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    34321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    34381 ccaattcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    34441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    34501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    34561 agcccggcgc ttctgtagtt ttagtttcgg aacccataca aaaatgctat ccatcttaaa
    34621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    34681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    34741 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    34801 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    34861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    34921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    34981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    35041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    35101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    35161 gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    35221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    35281 gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc aactgcggat
    35341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    35401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    35461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    35521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    35581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    35641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    35701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    35761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    35821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    35881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    35941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    36001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    36061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    36121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    36181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    36241 tcggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    36301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    36361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    36421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    36481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    36541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgtc
    36601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    36661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    36721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    36781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    36841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    36901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    36961 tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    37021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    37081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    37141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    37201 acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    37261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    37321 tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    37381 acatctcgcg gcgattatcg acggcgtgga agataaatcc cgcgtcggcg tctgcattga
    37441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac
    37501 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    37561 tgcgaaaagt acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    37621 cggtcatgat gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat
    37681 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    37741 aactgaaaaa gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt
    37801 acggcgtaac ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc
    37861 acgtgttgcc gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata
    37921 tatccttacc gagcaggatt actgtgtggt tgtgggtgca atcaatatgg atattcgcgg
    37981 aatggcggat atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc
    38041 aggcggcgtc ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt
    38101 aatatcagct attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc
    38161 gggcgtgaac gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc
    38221 tatcgctaat aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca
    38281 gctaacgcct caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct
    38341 ggctgattgt aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat
    38401 tccgatgttt gtcgataccg tttccgaatt taaagcgaac aaagtaaaaa actggtacag
    38461 ccggatccac actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat
    38521 taaagacgac aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg
    38581 catttttgtt tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt
    38641 tttgctgacc gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    38701 ggcgggcctg gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc
    38761 ggtagcctgc gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc
    38821 cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac
    38881 cgcatcttat ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata
    38941 gtagcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg
    39001 atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    39061 ccaaagttag cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga
    39121 gtgccacccg tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc
    39181 agcttctgcc caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc
    39241 cacgccaatg gggccaacag gtaacctaaa atcgactcca gcgaagcatg ttcaaaacca
    39301 aaccagccgc caacaccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc
    39361 ataacaactg tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    39421 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa agaaacctgt
    39481 gaagattccg tagccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt
    39541 aacgatgccg ccagcaaata ttccacaggt acgcccagtg cggcgtaacc aatcattgtc
    39601 gaaccagcaa tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga
    39661 cgatcgataa agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg
    39721 accgcgacga atgactcgat cttgctgata tttaaagctt tctggaagat accgccgaga
    39781 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg
    39841 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    39901 tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacctgcgtc gctgtacgcc
    39961 atcactttgt ggacgccaaa agcgactttt tctgcgaccc aacgccctgg cggtaaccaa
    40021 agcataatcc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    40081 atcttcttct tgtttactga cagtaaaaac gcaattgcca gcagtaccac cattcccaga
    40141 acacttctca tgacatccat agtgattatc tcttcatgtc attttgggcg acggctttct
    40201 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    40261 taatgggttt ttatgtagcc gcgtacgcac tcttcgacta atccccagaa ccagtcggta
    40321 tcaatagtga tgccgacttt agtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    40381 acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt
    40441 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca
    40501 gccaggccgt agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacatc
    40561 tctccggcag ggccgcctgc cctttccatt cgtgcaatta cgtccggggt gcaaacggtc
    40621 tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    40681 gcggcttccg ggtcggcaaa gatattgaac tcggcagatg gggtgaagtt gcctgtaccg
    40741 taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg ttgcatacgc
    40801 attgccaccg cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt
    40861 gccataaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc
    40921 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    40981 tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg
    41041 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc aacaatggtg
    41101 atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat agcatcatca
    41161 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    41221 cagttgcgtt attttttcta cagcaacgaa atttcgatcg ggccgatgat tgaatcttaa
    41281 caacagcgta cgaatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    41341 tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt
    41401 taacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    41461 aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg
    41521 ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg
    41581 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    41641 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    41701 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    41761 ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg
    41821 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc
    41881 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    41941 aagggtatct aattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg
    42001 agaataaatt ctccgggatg atgcagtaaa aattattggc aatagcacga ttgccggatg
    42061 caacgcttaa taaggcgtac atccggcaat gaaactcaag ctaaaccaat aaagaacccg
    42121 gcaatggtcg cgctcatcag gttggaaagc gtcgccgccg ccagcgcccg taaaccaagc
    42181 tgggcgattt ccggcgcgcg ttttggcgaa atagccgaaa acgctccaac gacaacaccg
    42241 atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat ggtcttgaca
    42301 tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac aaactcatta
    42361 atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca gtccaccccc
    42421 ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga gacattggca
    42481 aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat gatcgctaca
    42541 aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat tgcaccgttc
    42601 gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt ttcaaacgta
    42661 acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc gccaggaata
    42721 gccatcagcg aagcggctaa caaatagtca atcggcacgc ccattccggc atagccaatc
    42781 atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag ctcgttgcga
    42841 ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc gaggaaaata
    42901 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg aaaaatactg
    42961 ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct gataagcgcg
    43021 gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc accgtcaaac
    43081 agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc tgcgtcactg
    43141 taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt tcccggggga
    43201 aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc aaccgtgcgc
    43261 aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa taccaccatc
    43321 cccgcaacac ttctcattat atccataatg attttccctt catgccagta aacccggcgt
    43381 cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc cgccagaaat
    43441 taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc gttattaaag
    43501 accagttgaa tattggattt caggctatca ccgccggtta gctccgcaac acgcgccagc
    43561 aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc aacggcttga
    43621 tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat cgggttcgct
    43681 accaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg agcaatttcg
    43741 cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa aaacgcaggc
    43801 agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata ttctgttgtt
    43861 aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt ggtatttgcc
    43921 agttcctgta aatcggcaga aatatcgaag gtatgttccg ctccgcgatg cacaccacca
    43981 atacctccgg ttgcaaaaac tttaatccca gccagtgctg caataatcat ggttgatgca
    44041 acggtagtcg cgccattttt ccctgcagca acgacaaaag gtaaatcacg acgactaact
    44101 ttcgtcacgt tatgtccttc acggcccagc aattcgattt cttctttact taagcccact
    44161 ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt acgaattgtt
    44221 tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg agaaattatg
    44281 gtcgattcca gcgcaacaac cggtttttta ttttttaaag catcctgtac ttcagcggat
    44341 atttgtaata attcagggga cagagttaat tcagacattc tgtgttctcc actaacgatc
    44401 taacattggc aattgataat tcagggttgt tggtatattc acaggcaagt gccattgacg
    44461 aacatccctg cgcaaatcga acagaatcga taaacggcat accgtctacc caacaggagg
    44521 ccagccccgc catcatggcg tcgcctgccc cagtaacatt gatgacattc gttttaatag
    44581 gggccgacca cccactttca ccgttgatat cactgtagta aacgccatcc cctcccatgc
    44641 tcagcaccaa gcggttcagg ccgtgttgat gaaaccaggc ggcgaccttt gcgacatctt
    44701 cacgtccgga aagtgcaatt ccgctcaacg tttcggcttc aagacggtta ggttttaacg
    44761 tatggatttt gctcagatga tcgcggattt tgacgcattt ccatgccgat accggatcaa
    44821 taaatacagg cgtttccccg ctattttcca gaacccagac cagcgcctct tcactaaggt
    44881 tgcaatccgc gacaataacc tttgcaccgc gaataaactc ctgatgctgg gcaagaaatt
    44941 ctgcagaaat acagtcacta atactcatat catttatcgc gacaagcatt tctcctgtat
    45001 tatcgagcag tgataaatag ctggatgtat tctcacccgg aacaattaag catttatcga
    45061 cataaacacc ggattgatta gtttgcgcca gtaatgattg cccataaaaa tcaccaccta
    45121 ctgcacttaa aagccaggca tttttcccca gtaacgccag attatgagcg atattccttc
    45181 ccacgccacc tggggtaaat ttaatttttc cgggattcga atccgcataa ttcaatggat
    45241 gatgtgaata cccagccaca tcaatattcg ccgaaccaat aattactatg tagtcctttt
    45301 cgcgcatagc acgccccctg gcaataaatt gccaacgaag aaattgggta aaatagccat
    45361 tacagtcaat aagactatta gagcacgtgt tcagatttaa acacatgttc ataataagca
    45421 tatttaacgt aatgtaaatc gatcagaaaa aacgaaatcg gccagatatg aaggcggtca
    45481 caaagtttgt ctatttatgc agcaacaaaa gagaaagttg tgatgtgtaa cacacaatat
    45541 ggcaggcaag aaaaagggca gagatgtttc tgccctccag attgttgaca acgcgcgctt
    45601 tgttcatcct ggatgtggcg taaacgcctt atccgaccta caaaagcgca aaaaattcaa
    45661 tatattgcag ggttcatgta ggtctgataa gcatagcgca tcaggcaatt ttgcgtttgt
    45721 catcatcggg gcagagatgt ctcatccccg ataatgaaga taaattatgc gtttttttct
    45781 actatttgtg tttccgggcg tttcaggaag gcataggcca aacccgccac cagcgtaccg
    45841 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
    45901 aacagaccac cgtgtggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
    45961 agcgccccac caacgataca gcacggtagc acacgcatcg gatcacgagc agcaaacgga
    46021 attgcacctt ccgaaataaa gcacagacca agtaccagag cggctttgcc accttcctgc
    46081 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca gacccattgc cagcggtggc
    46141 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
    46201 acaccgaatg cgtacgctgc tttgtttacc ggaccgccca tgtcagtaca catcatgcca
    46261 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
    46321 aacccatcga gaatgccagc aaccggttta ccgattaggt agatcatcgc cagaccgacc
    46381 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
    46441 agtttcaatt gcgtactgat taacttcgca atgtaaccag ccaggaagcc agcaattata
    46501 ccgccaataa agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggtgtg
    46561 aggcccggac gatcggcaat ggagaaggca atataacctg ccagtaccgg caccatcagc
    46621 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
    46681 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacaaccatc
    46741 ggcagcatgt aagagacgcc cgtcagcaag tgacggtaag cgcctgcact ctctttctta
    46801 ccttcagtgg tcgctgtttg agctttgccc gctggttcat acggcgttgc ttcagcaacc
    46861 gctttatcca gttcctgcgc agttttcttc agcgccagac cggtagaggt gcgatacatc
    46921 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
    46981 gctgctgcga cttcttcagg ggtgattgca ttgcccgcgc caacagaacc acgggtttca
    47041 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
    47101 gtgtgtgcta cgccagtcgg gcaagcagtc accgcaacta cgcgtttcgg accgctggca
    47161 gcaaccggtg ctgtcgcagc gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
    47221 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
    47281 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
    47341 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
    47401 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
    47461 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
    47521 cggacgatcg gtaataccca cattgctttg acttaccgcc agggctgcaa cagctgtcgc
    47581 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
    47641 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
    47701 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
    47761 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
    47821 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
    47881 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
    47941 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
    48001 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
    48061 gtcggcgagg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
    48121 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
    48181 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
    48241 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
    48301 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
    48361 aacaaggtca taagccggat taagggtgat cgtagcaaca cgtctgctca ttatgcgccc
    48421 tccccaagac cagcagcgat agcgtcgcca atggctttca gcgcctgttc agcatctgca
    48481 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
    48541 agactacgtc cgtttgccgg tttgccggtg ccatcaaggt ttgtcacggt aatatcactg
    48601 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
    48661 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
    48721 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
    48781 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
    48841 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
    48901 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
    48961 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccac ctctttcaga
    49021 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
    49081 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
    49141 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
    49201 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
    49261 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
    49321 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
    49381 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
    49441 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
    49501 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
    49561 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
    49621 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
    49681 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgtcattt ttcctaattg
    49741 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
    49801 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
    49861 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgacctgcat gcataactcc
    49921 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
    49981 tttctcaccg gtattgcggg tgctctgcaa accccgacac tcagtatttt ccttaccgat
    50041 gaagtacatg cccgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
    50101 attctggtaa gtcagtttct cgctgggcgc tctgataagc gcggcgatcg caaatcgctg
    50161 attgtctttt gctgcctgtt aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac
    50221 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
    50281 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
    50341 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
    50401 gcgttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
    50461 gtttgcggcg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
    50521 accggcacgg ttgaagcgcc gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt
    50581 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
    50641 aacgaactgc atcttcccga gaaactggcc gatgtgatga tggggaccgc cgccgggctg
    50701 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
    50761 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
    50821 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
    50881 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
    50941 tataccaaca cttcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
    51001 gaaatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
    51061 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
    51121 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
    51181 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
    51241 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
    51301 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
    51361 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
    51421 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
    51481 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
    51541 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
    51601 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
    51661 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
    51721 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
    51781 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    51841 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    51901 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    51961 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    52021 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    52081 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    52141 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    52201 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    52261 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    52321 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    52381 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    52441 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    52501 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
    52561 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    52621 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    52681 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    52741 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    52801 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    52861 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaatgttc gccagaactc
    52921 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    52981 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
    53041 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
    53101 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    53161 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    53221 ttcgtccata tcagtgattg catgcaggat cgcgtatttc gccatgctgc cagaacatta
    53281 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    53341 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
    53401 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    53461 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    53521 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    53581 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    53641 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    53701 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    53761 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    53821 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    53881 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    53941 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    54001 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    54061 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    54121 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    54181 gatctgttaa ccgcaccagt ctatgaactg tggatagatc tgcgcgccac cttgtgcatt
    54241 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    54301 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    54361 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    54421 catgggaaag ttgactgtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    54481 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    54541 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    54601 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    54661 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    54721 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    54781 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    54841 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
    54901 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt
    54961 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
    55021 tctttaatca gaaactggtc gaaagtaagg cctttttgtg gctggttgca ttgaccaaca
    55081 atcgcgcttt cgacggttgt tctctgctgg cgatgggaat gttgatgctg agtttctggc
    55141 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
    55201 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
    55261 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
    55321 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
    55381 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
    55441 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
    55501 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
    55561 ccttttttga caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag
    55621 taattaacat catcaggctt atttttatag tgaggataat cctgatgatg cgcaccgtgc
    55681 tttcatccat cgcgcgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
    55741 ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat
    55801 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
    55861 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
    55921 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgccgcttg gcattttttt
    55981 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
    56041 cgattttgag atatatcttg cgcgggatcc ccgcgattgc agtagcggtt ctgctttctg
    56101 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
    56161 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
    56221 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
    56281 gcagcactaa aaaaggtatc gattgttctg gttttgtaca acgtacattc cgtgagcaat
    56341 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
    56401 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
    56461 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
    56521 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
    56581 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
    56641 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
    56701 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
    56761 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
    56821 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
    56881 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
    56941 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
    57001 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
    57061 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
    57121 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
    57181 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
    57241 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
    57301 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
    57361 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
    57421 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
    57481 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc
    57541 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
    57601 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgagtaacg ggcctggaag
    57661 tactgctacg ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact
    57721 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
    57781 gcgatgccgc agaattagaa aaagtgctgc cagtaggcgt caaatttggt attaacattg
    57841 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
    57901 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
    57961 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
    58021 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
    58081 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
    58141 acgcggtgct gacgctggcg aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa
    58201 cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
    58261 ttagtcgccc tttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc
    58321 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
    58381 aatgatgagt aaaaggaaat ccattgcaga tgattgtgcg catactgctg ctgtttatcg
    58441 ctctgttcac ctttggtgtg caggcgcagg ctatcaagga aagctatgcc tttgccgtgc
    58501 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
    58561 caaaaggtgg gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg
    58621 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt
    58681 ccgatgacga accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg
    58741 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca
    58801 ttactgcccg cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat
    58861 ttcgtctggt ctacaaaggc accaccgtca aagccattgc accgttaacc gtgcgcattg
    58921 agttagctaa acccagcaaa gaagatatgc tgagtctgtt ttcgctgccg atatttccag
    58981 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
    59041 gtccgtaccg cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
    59101 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
    59161 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
    59221 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
    59281 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
    59341 cgtttaatat ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcactctcg
    59401 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
    59461 gttattttca gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
    59521 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
    59581 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
    59641 acgaaacggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
    59701 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
    59761 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
    59821 ctaaccgcat gcgcagtcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
    59881 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
    59941 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
    60001 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
    60061 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
    60121 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
    60181 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
    60241 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
    60301 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
    60361 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
    60421 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagac ccagaagtga
    60481 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
    60541 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
    60601 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
    60661 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
    60721 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
    60781 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
    60841 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
    60901 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
    60961 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
    61021 aaggggtaag tgagaaaaac attctctgga aacatgtgtt ccgcaacgcc atgctgctgg
    61081 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    61141 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    61201 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    61261 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    61321 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    61381 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    61441 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    61501 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    61561 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    61621 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    61681 ctggctggga acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg
    61741 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    61801 gggggcgcta caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga
    61861 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    61921 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    61981 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    62041 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    62101 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    62161 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    62221 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    62281 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    62341 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    62401 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    62461 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    62521 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    62581 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    62641 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    62701 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    62761 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    62821 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    62881 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    62941 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    63001 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    63061 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    63121 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    63181 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    63241 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    63301 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    63361 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    63421 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    63481 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    63541 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    63601 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    63661 cattaattct taagccctca ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    63721 cggtacaggc gcaaatattg acgctattga aatcattgca acaaaagcat caactggcct
    63781 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    63841 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc gcaccgcagc
    63901 aggagtatac gcgtcagcta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    63961 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    64021 tggcgattta caaacaggca cggctcacct tcacactcca gcaccgaaca tggaacttcg
    64081 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    64141 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    64201 tcaaccttcg aacctgcgaa accagcagac cagcgaaagt tctgcggtag acgatggacg
    64261 attgagagtt gtaatgaagt cacttatttt ccccggaagc acatttactt cacaatttat
    64321 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    64381 cctcgcctgc atggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    64441 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    64501 gactttttac ataaattgat tttacataaa ataaacatat atcgggggaa tgatagattt
    64561 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
    64621 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    64681 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    64741 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    64801 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
    64861 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    64921 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    64981 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    65041 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
    65101 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
    65161 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
    65221 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    65281 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    65341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    65401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    65461 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
    65521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    65581 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
    65641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    65701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    65761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    65821 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
    65881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    65941 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
    66001 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
    66061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    66121 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
    66181 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
    66241 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    66301 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
    66361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    66421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    66481 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
    66541 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
    66601 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
    66661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
    66721 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
    66781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    66841 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
    66901 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    66961 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    67021 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    67081 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
    67141 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
    67201 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
    67261 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
    67321 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
    67381 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
    67441 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
    67501 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
    67561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    67621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    67681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    67741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    67801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    67861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    67921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    67981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    68041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    68101 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
    68161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
    68221 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
    68281 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
    68341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    68401 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    68461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    68521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    68581 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
    68641 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
    68701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    68761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt
    68821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    68881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    68941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
    69001 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    69061 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
    69121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    69181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    69241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    69301 gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgtgttca atccttcgct
    69361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    69421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    69481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    69541 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
    69601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    69661 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    69721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    69781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    69841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    69901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    69961 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    70021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    70081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
    70141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    70201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    70261 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
    70321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    70381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    70441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    70501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
    70561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    70621 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    70681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    70741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    70801 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    70861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    70921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    70981 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
    71041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
    71101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    71161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    71221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    71281 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    71341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    71401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    71461 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
    71521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    71581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
    71641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
    71701 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
    71761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
    71821 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
    71881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    71941 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    72001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    72061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    72121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    72181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    72241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
    72301 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
    72361 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
    72421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    72481 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
    72541 aactttcaca tggttagaat cggcctattc ctgacgcacg gcggctttca gccagggttc
    72601 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    72661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    72721 atctgtggta aagccggtaa aggcgagata ggggctaaca ctccacaatc cgtcaaccca
    72781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    72841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    72901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
    72961 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
    73021 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    73081 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    73141 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
    73201 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
    73261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    73321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    73381 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
    73441 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
    73501 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
    73561 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    73621 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    73681 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    73741 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    73801 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
    73861 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    73921 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
    73981 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
    74041 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
    74101 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
    74161 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    74221 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
    74281 ccgccactat ccacagtggt attataagcg aagtcatttt cttcaactcg caaacttccg
    74341 ccgttttcca acagcatatt gttagcaata ccattcttga tttcgaactg accgagacgg
    74401 tttgttccga atacctccat gacccgcgtg gttgttttaa tagcaccgcc tgctttctga
    74461 tctaccgcaa atgctaatcc cccgtcttta acctccagta atcctccctt ttcgatagcg
    74521 gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat cgtgggatca
    74581 ttcgccgcg
//
LOCUS       DAZRVY010000004        72510 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZRVY010000004 DAZRVY010000000
VERSION     DAZRVY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.004"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXOX_000232"
     CDS             complement(<1..215)
                     /locus_tag="ACHXOX_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7003815.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXOX_000233"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXOX_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR7003816.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXOX_000234"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXOX_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR7003817.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXOX_000235"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXOX_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR7003818.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXOX_000236"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXOX_000236"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR7003819.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXOX_000237"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXOX_000237"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR7003820.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXOX_000238"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXOX_000238"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR7003821.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHXOX_000239"
     CDS             complement(4604..5326)
                     /locus_tag="ACHXOX_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7003822.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXOX_000240"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXOX_000240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7003823.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHXOX_000241"
     CDS             6526..6744
                     /locus_tag="ACHXOX_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR7003824.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXOX_000242"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXOX_000242"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR7003825.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHXOX_000243"
     CDS             complement(7478..7678)
                     /locus_tag="ACHXOX_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR7003826.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXOX_000244"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXOX_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR7003827.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXOX_000245"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXOX_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR7003828.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHXOX_000246"
     CDS             10175..12088
                     /locus_tag="ACHXOX_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7003829.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHXOX_000247"
     CDS             12088..13110
                     /locus_tag="ACHXOX_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR7003830.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHXOX_000248"
     CDS             13104..13322
                     /locus_tag="ACHXOX_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR7003831.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHXOX_000249"
     CDS             13376..14245
                     /locus_tag="ACHXOX_000249"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR7003832.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHXOX_000250"
     CDS             complement(14300..14704)
                     /locus_tag="ACHXOX_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR7003833.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHXOX_000251"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHXOX_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR7003834.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHXOX_000252"
     CDS             15689..17779
                     /locus_tag="ACHXOX_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR7003835.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXOX_000253"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXOX_000253"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR7003836.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXOX_000254"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXOX_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR7003837.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHXOX_000255"
     CDS             complement(19747..20349)
                     /locus_tag="ACHXOX_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR7003838.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHXOX_000256"
     CDS             complement(20339..20506)
                     /locus_tag="ACHXOX_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR7003839.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXOX_000257"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXOX_000257"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR7003840.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXOX_000258"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXOX_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR7003841.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXOX_000259"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXOX_000259"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR7003842.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXOX_000260"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXOX_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR7003843.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXOX_000261"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXOX_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR7003844.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXOX_000262"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXOX_000262"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR7003845.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="ACHXOX_000263"
     CDS             28334..28501
                     /locus_tag="ACHXOX_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR7003846.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28796..30133
                     /gene="frlA"
                     /locus_tag="ACHXOX_000264"
     CDS             28796..30133
                     /gene="frlA"
                     /locus_tag="ACHXOX_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR7003847.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="ACHXOX_000265"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="ACHXOX_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR7003848.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKVKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="ACHXOX_000266"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="ACHXOX_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="ACHXOX_000267"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="ACHXOX_000267"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR7003849.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="frlR"
                     /locus_tag="ACHXOX_000268"
                     /gene_synonym="yhfR"
     CDS             32939..33670
                     /gene="frlR"
                     /locus_tag="ACHXOX_000268"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR7003850.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="ACHXOX_000269"
     CDS             complement(33822..34907)
                     /locus_tag="ACHXOX_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR7003851.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="ACHXOX_000270"
     CDS             complement(34919..36223)
                     /locus_tag="ACHXOX_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR7003852.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="ACHXOX_000271"
     CDS             complement(36235..36588)
                     /locus_tag="ACHXOX_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR7003853.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="ACHXOX_000272"
     CDS             complement(36599..37477)
                     /locus_tag="ACHXOX_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR7003854.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="ACHXOX_000273"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="ACHXOX_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="ACHXOX_000274"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="ACHXOX_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="ACHXOX_000275"
     CDS             complement(39947..40309)
                     /locus_tag="ACHXOX_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR7003855.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHXOX_000276"
     CDS             complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHXOX_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR7003856.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="ACHXOX_000277"
     CDS             complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="ACHXOX_000277"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR7003857.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42388..43146)
                     /gene="gph"
                     /locus_tag="ACHXOX_000278"
     CDS             complement(42388..43146)
                     /gene="gph"
                     /locus_tag="ACHXOX_000278"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR7003858.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="ACHXOX_000279"
     CDS             complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="ACHXOX_000279"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR7003859.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43834..44670)
                     /gene="dam"
                     /locus_tag="ACHXOX_000280"
     CDS             complement(43834..44670)
                     /gene="dam"
                     /locus_tag="ACHXOX_000280"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR7003860.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44777..46063)
                     /gene="damX"
                     /locus_tag="ACHXOX_000281"
     CDS             complement(44777..46063)
                     /gene="damX"
                     /locus_tag="ACHXOX_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR7003861.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="ACHXOX_000282"
     CDS             complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="ACHXOX_000282"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR7003862.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="ACHXOX_000283"
     CDS             complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="ACHXOX_000283"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR7003863.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="ACHXOX_000284"
     CDS             complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="ACHXOX_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR7003864.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="ACHXOX_000285"
     CDS             complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="ACHXOX_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR7003865.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="ACHXOX_000286"
     CDS             complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="ACHXOX_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR7003866.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="ACHXOX_000287"
     CDS             complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="ACHXOX_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR7003867.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="ACHXOX_000288"
     CDS             complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="ACHXOX_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR7003868.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51628..54180
                     /gene="mrcA"
                     /locus_tag="ACHXOX_000289"
     CDS             51628..54180
                     /gene="mrcA"
                     /locus_tag="ACHXOX_000289"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR7003869.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="ACHXOX_000290"
     CDS             complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="ACHXOX_000290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR7003870.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55225..57360
                     /gene="igaA"
                     /locus_tag="ACHXOX_000291"
     CDS             55225..57360
                     /gene="igaA"
                     /locus_tag="ACHXOX_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR7003871.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57425..58093
                     /gene="yrfG"
                     /locus_tag="ACHXOX_000292"
     CDS             57425..58093
                     /gene="yrfG"
                     /locus_tag="ACHXOX_000292"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR7003872.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58104..58505
                     /gene="hslR"
                     /locus_tag="ACHXOX_000293"
     CDS             58104..58505
                     /gene="hslR"
                     /locus_tag="ACHXOX_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR7003873.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58530..59408
                     /gene="hslO"
                     /locus_tag="ACHXOX_000294"
     CDS             58530..59408
                     /gene="hslO"
                     /locus_tag="ACHXOX_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR7003874.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59543..61243)
                     /locus_tag="ACHXOX_000295"
                     /pseudo
     CDS             complement(59543..61243)
                     /locus_tag="ACHXOX_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            61622..63244
                     /gene="pckA"
                     /locus_tag="ACHXOX_000296"
     CDS             61622..63244
                     /gene="pckA"
                     /locus_tag="ACHXOX_000296"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR7003875.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="ACHXOX_000297"
     CDS             complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="ACHXOX_000297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR7003876.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="ACHXOX_000298"
     CDS             complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="ACHXOX_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR7003877.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65616..66092
                     /gene="greB"
                     /locus_tag="ACHXOX_000299"
     CDS             65616..66092
                     /gene="greB"
                     /locus_tag="ACHXOX_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR7003878.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66189..68510
                     /locus_tag="ACHXOX_000300"
     CDS             66189..68510
                     /locus_tag="ACHXOX_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR7003879.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68948..69175
                     /gene="feoA"
                     /locus_tag="ACHXOX_000301"
     CDS             68948..69175
                     /gene="feoA"
                     /locus_tag="ACHXOX_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR7003880.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69192..71513
                     /gene="feoB"
                     /locus_tag="ACHXOX_000302"
     CDS             69192..71513
                     /gene="feoB"
                     /locus_tag="ACHXOX_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR7003881.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71513..71749
                     /gene="feoC"
                     /locus_tag="ACHXOX_000303"
     CDS             71513..71749
                     /gene="feoC"
                     /locus_tag="ACHXOX_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR7003882.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71952..>72510
                     /locus_tag="ACHXOX_000304"
     CDS             71952..>72510
                     /locus_tag="ACHXOX_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR7003883.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
BASE COUNT        17432 a        19699 c        18349 g        17030 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 accgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaaggt caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat accgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa ctattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc gtgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41281 aagtggcaac gacgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca
    41341 aatgaggata aaaaaaccgg gtttccccgg tttttggcat taatgttaaa ttacggcttc
    41401 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    41461 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    41521 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    41581 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    41641 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    41701 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    41761 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    41821 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    41881 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    41941 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    42001 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    42061 tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tataacagtt cagtgcccag
    42121 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    42181 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    42241 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    42301 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    42361 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    42421 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    42481 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc
    42541 cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc
    42601 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    42661 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg
    42721 tgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    42781 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    42841 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    42901 atcaacgggc ggtttaccca ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt
    42961 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    43021 acctgcgatg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccggg
    43081 agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    43141 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    43201 gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc
    43261 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    43321 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    43381 cgaaaccagg attgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    43441 gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    43501 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    43561 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    43621 cgataggggc ggtaatgcca tagttacgca aggatttcag caccattggc ccaatcgtca
    43681 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    43741 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    43801 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    43861 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    43921 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    43981 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    44041 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    44101 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    44161 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    44221 gtgatacaac tctgcttccg ggaaataggt ttttttgtag cggccaaacg gcacgttaaa
    44281 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    44341 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    44401 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    44461 agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    44521 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    44581 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    44641 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    44701 gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg
    44761 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    44821 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    44881 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgcc gtttcataga
    44941 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    45001 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    45061 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    45121 ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagtc ggtgccttcg
    45181 tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct
    45241 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctgggc
    45301 gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat
    45361 tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt ggaattgacc gccacattgt
    45421 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    45481 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    45541 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    45601 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    45661 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    45721 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    45781 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    45841 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    45901 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    45961 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    46021 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    46081 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    46141 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    46201 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    46261 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    46321 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    46381 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    46441 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    46501 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    46561 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    46621 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    46681 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    46741 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    46801 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    46861 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    46921 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    46981 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    47041 ataacgctat cgacattaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    47101 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    47161 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    47221 agagtaacaa caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    47281 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    47341 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    47401 tgcgttcatt ggccaacgct tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg
    47461 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    47521 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccaccgc
    47581 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    47641 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    47701 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    47761 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    47821 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    47881 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    47941 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    48001 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    48061 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    48121 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    48181 aaacacgttc agcgtaaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    48241 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    48301 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    48361 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    48421 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    48481 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa cactgtgggc
    48541 gtaacttcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    48601 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    48661 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc
    48721 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    48781 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    48841 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    48901 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    48961 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    49021 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    49081 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    49141 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    49201 ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    49261 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    49321 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    49381 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    49441 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    49501 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    49561 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    49621 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    49681 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    49741 tacaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    49801 acaaaagatc atcaccttcc acgcttaatg aaaaacggct cacgctgacg ttgcgctctg
    49861 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    49921 cctgcgcgga tggatgccag gaaaccagtt gcgcgccggg caactgaaaa tccagtggcg
    49981 aaaagggcag cgttttttct tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg
    50041 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50101 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50161 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50221 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50281 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50341 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50401 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50461 tgtcgctggc gctgccgttg caaacgctgc tcacgaattt gttggcgctc cagcaaacgt
    50521 ggtttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aacggtgttt
    50581 acgcgtgctt cggcgttgtc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50641 agaggcgcaa cgaacatcag caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc
    50701 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    50761 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50821 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    50881 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    50941 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51001 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51061 acctttctgc cagagtgagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51121 aggcgggact gagtgagtct tcgctgtaat cgaaacgcag ggagtccgga tccatatcca
    51181 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcaccg agggacatcg
    51241 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51301 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagccgc tgcgcatcaa
    51361 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    51421 agcattcttt tgcgccccgc acgatcgcaa ccgctactgc ttcttgctgt tgtaaatgca
    51481 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51541 cgggtatatc aatgcgtctg gcttgccttt atactaccgc acgtttgttt ataaactgcc
    51601 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51661 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51721 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51781 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51841 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51901 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    51961 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52021 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52081 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52141 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52201 acgctgaacg aaatggctgt gatagccggg ctgccgaaag cgccttccac ctttaatccg
    52261 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52321 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52381 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52441 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52501 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52561 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52621 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52681 gccgcagtca ccagcgccaa tcctcaggaa gcgacggcaa tgctggcgga cgggtcgact
    52741 gtcgcattga gtatggacgg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52801 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52861 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52921 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    52981 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53041 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53101 tctcgctggg atgcaggtgt cggttctgac tggcagccga agaactcacc accgcagtat
    53161 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53221 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53281 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53341 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    53401 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53461 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53521 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53581 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53641 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53701 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53761 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53821 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53881 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    53941 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54001 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54061 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54121 gcggtgcacg aggtgggaac aaccattatc gataatggtg aggcacagga attgttctga
    54181 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54241 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54301 ccataagcct gataatcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54361 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54421 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54481 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54541 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54601 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54661 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54721 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54781 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54841 aacagtcggg aatgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54901 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    54961 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatt gtcctcaaat
    55021 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatatcc cgccttcaga
    55081 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55141 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55201 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55261 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55321 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55381 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55441 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55501 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55561 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55621 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55681 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55741 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55801 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55861 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55921 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    55981 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56041 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56101 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56161 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56221 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56281 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56341 ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56401 ggcgatacat tgcgtatcag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56461 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac
    56521 gcccgctcat tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56581 gcagttaatc gccagctgca ccctaaaccg gaagatggat ctcgcgtcag tgcctcatta
    56641 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56701 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56761 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56821 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    56881 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    56941 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57001 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57061 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    57121 actgacgcca atggtacgca gcatattggc cttcatccaa tcccggatcg ttccggcctg
    57181 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57241 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    57301 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57361 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57421 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57481 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57541 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57601 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57661 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    57721 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    57781 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    57841 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57901 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    57961 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58021 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58081 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58141 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58201 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58261 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58321 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58381 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58441 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58501 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58561 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58621 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58681 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    58741 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58801 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58861 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    58921 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    58981 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59041 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59101 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59161 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59221 cctgctcgcg tgaacgttgc gccgacgcgc tgaaaacgct gcctgatgaa gaagtcgata
    59281 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59341 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59401 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    59461 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    59521 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59581 ccgtcagatc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    59641 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    59701 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    59761 gatcttgctc gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat
    59821 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccagga gcaattaaca
    59881 cattttcggc taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg
    59941 ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    60001 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt
    60061 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    60121 tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa
    60181 ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa
    60241 aataccccag cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    60301 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    60361 gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    60421 acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta
    60481 acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggtccgta aacggcaggg
    60541 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg
    60601 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccacca
    60661 gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat
    60721 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    60781 tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt
    60841 aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta
    60901 gcaacaacat tgccatcaac agcagatatc agccaaaatc ccaaatcgcc ttttcagctc
    60961 tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa
    61021 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga
    61081 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt
    61141 ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa
    61201 tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc
    61261 aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc
    61321 aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca atttcattca
    61381 ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa
    61441 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    61501 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    61561 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    61621 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    61681 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    61741 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    61801 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    61861 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    61921 aacctggcag catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt
    61981 tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    62041 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    62101 ggcaggtttc aaactagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    62161 gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    62221 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    62281 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    62341 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    62401 cccgaaacgc cgcctgattg gcgatgacga acacggctgg gacgatgacg gcgtgtttaa
    62461 tttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    62521 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    62581 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc caatctatca
    62641 catcgataac atcgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct
    62701 gactgctgat gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca
    62761 gtatcacttc ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga
    62821 accgacgcca accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca
    62881 gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa
    62941 cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga
    63001 cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct
    63061 ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    63121 cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa
    63181 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact
    63241 gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc
    63301 accttcgcct cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca
    63361 ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc
    63421 cgttatggtt atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg
    63481 tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa
    63541 ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga
    63601 ctttgatcca gccattgcca taacgagcgg cgttgaccac catattcgcc accgcgcgtt
    63661 tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt
    63721 cccgctcata gccactttcg gcagcaatca cctcaccgag cactgcatta agatccgcca
    63781 tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt
    63841 tgcactcttc gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca
    63901 tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca
    63961 tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc
    64021 gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt
    64081 taccgacctg caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa
    64141 acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag
    64201 agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc
    64261 aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc
    64321 cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac
    64381 ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc
    64441 gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca
    64501 tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg
    64561 cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga
    64621 gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga
    64681 gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc
    64741 tggatcttct tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat
    64801 tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg
    64861 cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc
    64921 atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac
    64981 cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg
    65041 aatacgggcc agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat
    65101 ctccaggcct acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat
    65161 cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat
    65221 taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc
    65281 attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg
    65341 caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc
    65401 caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc
    65461 taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt
    65521 atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg
    65581 tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg
    65641 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
    65701 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
    65761 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca
    65821 tggaaaatct caaaatcgtc aattactccc ctcagcagga aggcaaagtc ttttttggcg
    65881 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
    65941 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
    66001 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
    66061 atgttaatgc tatcgaatac gtgaaaccgt aatgaagagt cttaacctcc tgccgatggc
    66121 tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa
    66181 ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc
    66241 cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg
    66301 cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa
    66361 cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt
    66421 ccgagcaagg caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa
    66481 ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg
    66541 ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacac
    66601 cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat accaaagccg
    66661 cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg
    66721 cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta
    66781 aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg
    66841 tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt
    66901 cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca
    66961 tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg
    67021 tagtgagctg gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta
    67081 ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc
    67141 tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta
    67201 ccggggtaaa agtggcagtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt
    67261 acccgcacac cggacaagcc gcaaaagtag cgatgaccgt tgctgccttg tgtgaaaaac
    67321 ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct
    67381 atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag
    67441 ctggcgcgtc ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg
    67501 tttcgctgcg tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg
    67561 tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc
    67621 aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc
    67681 tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc
    67741 aaaacatcgt ggcctggcgc gatgagaacg gccagttcaa gaaccgtcag caactgctga
    67801 aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc
    67861 acggtgataa cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac
    67921 gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc
    67981 gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca
    68041 tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt
    68101 ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg
    68161 tcaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg
    68221 ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg
    68281 gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga
    68341 ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac
    68401 gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta
    68461 atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa acgcccggac
    68521 tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct
    68581 gacataaaag tgccggagaa tatctccggc ctttttattc cacaaccaaa ctcataatat
    68641 attccggcaa tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc
    68701 cgggtaattc actattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca
    68761 ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa
    68821 ccattctcat tatcattgtg ttgttgatta tttaatctct cctttgttgg caaatcatct
    68881 ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta
    68941 ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat
    69001 cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa
    69061 tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct
    69121 ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg
    69181 tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat tctggcaaga
    69241 caaccttatt taaccagctc actagcgcac gtcagcgtgt aggtaactgg gctggcgtta
    69301 ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc
    69361 tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg
    69421 cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta
    69481 accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg
    69541 tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc
    69601 tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg
    69661 aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt
    69721 acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc
    69781 cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg
    69841 cctacgccgg tgaagcgtcg cagcatctgg atgccgccct tgcccgtctg cgtaatgaaa
    69901 tggatgatcc ggcgctgcac attgccgatg cgcgttacca gtgcatagct gccatctgtg
    69961 atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa
    70021 tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc
    70081 tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc
    70141 tatttgtgca tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta
    70201 tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga
    70261 tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcag
    70321 cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc
    70381 tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac
    70441 cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg
    70501 ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgccctg gcggttttct
    70561 cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca
    70621 tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg
    70681 ttaaaagcct gattatccag acctggcagc gtctgaaggg cttcgttctg cgtgctggta
    70741 aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga
    70801 aaatcgtcga taacatcaac gactcggcgc tggcgtccgt cagccgggtg ataacccctg
    70861 tcttcaagcc gattggcgta catgaagata actggcaggc aacggttggc ctgtttacgg
    70921 gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata
    70981 ttcaggacga agagttcaat ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg
    71041 tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca
    71101 ttgaagctag caaaggcgac ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga
    71161 aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccat
    71221 gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca
    71281 tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag gtcgccagct
    71341 acagtcagca cccaacttac agcctggtgt gcattctggc ggtaatcctg tttaacatcg
    71401 tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc
    71461 gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc
    71521 acttattcag gtacgtgatt tgctggcgtt acggggccgt atggaagcga cccagataag
    71581 ccagacattg aacactccgc agccaatgat taacgccatg ctgcaacaac tggaaagtat
    71641 gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag
    71701 ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat
    71761 cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc
    71821 ataaaaatgc aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga
    71881 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt
    71941 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    72001 ttttacgcca accggacacg gctcgtgatt ttcttgcgtt tcatttaccg gcacccattc
    72061 acgcgctttg tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc
    72121 tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca
    72181 tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc
    72241 gttacgctat tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg
    72301 tggtgctatt gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc
    72361 tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga
    72421 ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg
    72481 agttaattca aaaacatatt cgtcaacgcg
//
LOCUS       DAZRVY010000005        70303 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZRVY010000005 DAZRVY010000000
VERSION     DAZRVY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.005"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHXOX_000305"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHXOX_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR7003884.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHXOX_000306"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHXOX_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR7003885.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHXOX_000307"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHXOX_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR7003886.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHXOX_000308"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHXOX_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR7003887.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHXOX_000309"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHXOX_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR7003888.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHXOX_000310"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHXOX_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR7003889.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHXOX_000311"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHXOX_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR7003890.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHXOX_000312"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHXOX_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR7003891.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHXOX_000313"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHXOX_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHXOX_000314"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHXOX_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR7003892.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHXOX_000315"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHXOX_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR7003893.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHXOX_000316"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHXOX_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR7003894.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHXOX_000317"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHXOX_000317"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR7003895.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHXOX_000318"
     CDS             complement(14171..14743)
                     /locus_tag="ACHXOX_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR7003896.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHXOX_000319"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHXOX_000319"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR7003897.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHXOX_000320"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHXOX_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR7003898.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHXOX_000321"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHXOX_000321"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR7003899.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHXOX_000322"
     CDS             17912..18760
                     /locus_tag="ACHXOX_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR7003900.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHXOX_000323"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHXOX_000323"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR7003901.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHXOX_000324"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHXOX_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7003902.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHXOX_000325"
     CDS             complement(21672..21872)
                     /locus_tag="ACHXOX_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR7003903.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHXOX_000326"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHXOX_000326"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR7003904.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHXOX_000327"
     CDS             complement(23031..23387)
                     /locus_tag="ACHXOX_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR7003905.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="ACHXOX_000328"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="ACHXOX_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR7003906.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="ACHXOX_000329"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="ACHXOX_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR7003907.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="ACHXOX_000330"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="ACHXOX_000330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR7003908.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="ACHXOX_000331"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="ACHXOX_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR7003909.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="ACHXOX_000332"
                     /pseudo
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="ACHXOX_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            31858..32901
                     /locus_tag="ACHXOX_000333"
     CDS             31858..32901
                     /locus_tag="ACHXOX_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR7003910.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="ACHXOX_000334"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="ACHXOX_000334"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR7003911.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="ACHXOX_000335"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="ACHXOX_000335"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR7003912.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="ACHXOX_000336"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="ACHXOX_000336"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR7003913.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38832..39167
                     /gene="eutS"
                     /locus_tag="ACHXOX_000337"
     CDS             38832..39167
                     /gene="eutS"
                     /locus_tag="ACHXOX_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR7003914.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39180..39659
                     /gene="eutP"
                     /locus_tag="ACHXOX_000338"
     CDS             39180..39659
                     /gene="eutP"
                     /locus_tag="ACHXOX_000338"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR7003915.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39634..40335
                     /gene="eutQ"
                     /locus_tag="ACHXOX_000339"
     CDS             39634..40335
                     /gene="eutQ"
                     /locus_tag="ACHXOX_000339"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR7003916.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40332..41135
                     /gene="eutT"
                     /locus_tag="ACHXOX_000340"
     CDS             40332..41135
                     /gene="eutT"
                     /locus_tag="ACHXOX_000340"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR7003917.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41132..42148
                     /gene="pta"
                     /locus_tag="ACHXOX_000341"
     CDS             41132..42148
                     /gene="pta"
                     /locus_tag="ACHXOX_000341"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR7003918.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42187..42480
                     /gene="eutM"
                     /locus_tag="ACHXOX_000342"
     CDS             42187..42480
                     /gene="eutM"
                     /locus_tag="ACHXOX_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR7003919.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42587..42874
                     /gene="eutN"
                     /locus_tag="ACHXOX_000343"
     CDS             42587..42874
                     /gene="eutN"
                     /locus_tag="ACHXOX_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR7003920.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42886..44289
                     /locus_tag="ACHXOX_000344"
     CDS             42886..44289
                     /locus_tag="ACHXOX_000344"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR7003921.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44300..45136
                     /gene="eutJ"
                     /locus_tag="ACHXOX_000345"
     CDS             44300..45136
                     /gene="eutJ"
                     /locus_tag="ACHXOX_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR7003922.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45126..46313
                     /gene="eutG"
                     /locus_tag="ACHXOX_000346"
     CDS             45126..46313
                     /gene="eutG"
                     /locus_tag="ACHXOX_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR7003923.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46630..47856
                     /gene="eutH"
                     /locus_tag="ACHXOX_000347"
     CDS             46630..47856
                     /gene="eutH"
                     /locus_tag="ACHXOX_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR7003924.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47853..49256
                     /gene="eutA"
                     /locus_tag="ACHXOX_000348"
     CDS             47853..49256
                     /gene="eutA"
                     /locus_tag="ACHXOX_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR7003925.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49268..50629
                     /gene="eutB"
                     /locus_tag="ACHXOX_000349"
     CDS             49268..50629
                     /gene="eutB"
                     /locus_tag="ACHXOX_000349"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR7003926.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            50650..51537
                     /gene="eutC"
                     /locus_tag="ACHXOX_000350"
     CDS             50650..51537
                     /gene="eutC"
                     /locus_tag="ACHXOX_000350"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR7003927.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51547..52206
                     /gene="eutL"
                     /locus_tag="ACHXOX_000351"
     CDS             51547..52206
                     /gene="eutL"
                     /locus_tag="ACHXOX_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR7003928.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52219..52719
                     /gene="eutK"
                     /locus_tag="ACHXOX_000352"
     CDS             52219..52719
                     /gene="eutK"
                     /locus_tag="ACHXOX_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR7003929.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52765..53817
                     /gene="eutR"
                     /locus_tag="ACHXOX_000353"
     CDS             52765..53817
                     /gene="eutR"
                     /locus_tag="ACHXOX_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR7003930.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="ACHXOX_000354"
     CDS             complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="ACHXOX_000354"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR7003931.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="ACHXOX_000355"
     CDS             complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="ACHXOX_000355"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR7003932.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55809..56234
                     /locus_tag="ACHXOX_000356"
     CDS             55809..56234
                     /locus_tag="ACHXOX_000356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR7003933.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56221..56670
                     /locus_tag="ACHXOX_000357"
     CDS             56221..56670
                     /locus_tag="ACHXOX_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR7003934.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56731..57306
                     /locus_tag="ACHXOX_000358"
     CDS             56731..57306
                     /locus_tag="ACHXOX_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR7003935.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57402..58301
                     /gene="yfeX"
                     /locus_tag="ACHXOX_000359"
     CDS             57402..58301
                     /gene="yfeX"
                     /locus_tag="ACHXOX_000359"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR7003936.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="ACHXOX_000360"
     CDS             complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="ACHXOX_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR7003937.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59668..61092)
                     /gene="murP"
                     /locus_tag="ACHXOX_000361"
                     /pseudo
     CDS             complement(59668..61092)
                     /gene="murP"
                     /locus_tag="ACHXOX_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="ACHXOX_000362"
     CDS             complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="ACHXOX_000362"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR7003938.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62156..63013
                     /gene="murR"
                     /locus_tag="ACHXOX_000363"
     CDS             62156..63013
                     /gene="murR"
                     /locus_tag="ACHXOX_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR7003939.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63142..63933
                     /gene="ucpA"
                     /locus_tag="ACHXOX_000364"
     CDS             63142..63933
                     /gene="ucpA"
                     /locus_tag="ACHXOX_000364"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR7003940.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64104..65120
                     /gene="cysP"
                     /locus_tag="ACHXOX_000365"
     CDS             64104..65120
                     /gene="cysP"
                     /locus_tag="ACHXOX_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR7003941.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65120..65953
                     /gene="cysT"
                     /locus_tag="ACHXOX_000366"
     CDS             65120..65953
                     /gene="cysT"
                     /locus_tag="ACHXOX_000366"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR7003942.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65953..66828
                     /gene="cysW"
                     /locus_tag="ACHXOX_000367"
     CDS             65953..66828
                     /gene="cysW"
                     /locus_tag="ACHXOX_000367"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR7003943.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66818..67915
                     /gene="cysA"
                     /locus_tag="ACHXOX_000368"
     CDS             66818..67915
                     /gene="cysA"
                     /locus_tag="ACHXOX_000368"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR7003944.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68049..68960
                     /gene="cysM"
                     /locus_tag="ACHXOX_000369"
     CDS             68049..68960
                     /gene="cysM"
                     /locus_tag="ACHXOX_000369"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR7003945.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68963..69331)
                     /locus_tag="ACHXOX_000370"
     CDS             complement(68963..69331)
                     /locus_tag="ACHXOX_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR7003946.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69436..70287
                     /gene="pdxK"
                     /locus_tag="ACHXOX_000371"
                     /gene_synonym="thiJ"
     CDS             69436..70287
                     /gene="pdxK"
                     /locus_tag="ACHXOX_000371"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR7003947.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16701 a        18358 c        19437 g        15807 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggcgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gctaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gctcgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaaa
    38641 gcagtctata taatatctcg atattatttt atttatattc atgcgttgca tatgaaagtt
    38701 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
    38761 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
    38821 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
    38881 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
    38941 atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgtcggtg
    39001 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
    39061 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
    39121 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
    39181 tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt
    39241 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
    39301 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
    39361 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
    39421 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
    39481 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
    39541 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
    39601 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
    39661 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
    39721 attaccccgg aagcccgtaa agtggcggat ctactgggtt ttaccatcac cgaatgtgat
    39781 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
    39841 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
    39901 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
    39961 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
    40021 gtcaagtttg gtcgttttga tggcgcacaa ccgcattgcg tgggcttgac tgatcttgtg
    40081 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
    40141 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct ccatgttcgc
    40201 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
    40261 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
    40321 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
    40381 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
    40441 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
    40501 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
    40561 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
    40621 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
    40681 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
    40741 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
    40801 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc
    40861 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
    40921 cggcgttgct caatctgcta cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
    40981 tgtttatcac ccgcagcttt gaagtattac gcccggacat cttgcaggcg ctgaaccgcc
    41041 tttcgagcac ggtctacgta atgatgatcc tgagcgtaac gaagccgccg ctaacggtga
    41101 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
    41161 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gatcaacgtg tgctgaaagc
    41221 tgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcta atccgtttga
    41281 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
    41341 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
    41401 aaaaacgccg ccggatgcgc tggaaaaact taccgatccg ctgatgttcg ccgcagcaat
    41461 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcgaatgt
    41521 gctgcgtgcc ggattacgta ttatcggctt gcagccaggc tgtaaaacgc tctcatccat
    41581 tttcctgatg ctgccacagt acagcggtcc ggcgttgggc tttgccgatt gcagcgtggt
    41641 gccacagccg acggcggcgc aactggcgga tatcgcgctt gccagtgccg aaacctggcg
    41701 cgccctcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
    41761 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
    41821 aaagctggtg gttgatggcg agttgcagtt tgacgccgcc ttcgtgccgg aagtggcggc
    41881 gcaaaaagcg cctgccagcc cgctacaggg caaagccaat gtgatggttt ttccgtcgct
    41941 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
    42001 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
    42061 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga
    42121 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
    42181 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
    42241 tctgacgcga tggttaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
    42301 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
    42361 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
    42421 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
    42481 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
    42541 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
    42601 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
    42661 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
    42721 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
    42781 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
    42841 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
    42901 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
    42961 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
    43021 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
    43081 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
    43141 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
    43201 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgccc
    43261 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
    43321 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
    43381 aaagtctccc agcgggcgat cacgctgctc aaccaggcga ttgttgccgc aggcgggccg
    43441 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag
    43501 tttccgggca tcggccttct ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt
    43561 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
    43621 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
    43681 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
    43741 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
    43801 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
    43861 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
    43921 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
    43981 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
    44041 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
    44101 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgcg cattgccggg
    44161 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
    44221 gtaactagcg tgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
    44281 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
    44341 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
    44401 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agtcggtggc
    44461 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
    44521 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
    44581 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
    44641 gtctgctgga ctggaagtga gccatgtgct ggatgagcca accgcagttg cggatctgct
    44701 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
    44761 gaaaaagggc aaggtgacgt actcggcgga tgaagcaacc ggcggacatc acatctctct
    44821 gactctcgcc ggaaatcgcc gtatttcgct ggaagaggcg gagcagtaca agcgcggtca
    44881 cggtgaagag atttggccta cggtgaaacc ggtgtacgaa aaaatggcgg acattgtcgc
    44941 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
    45001 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
    45061 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
    45121 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
    45181 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
    45241 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
    45301 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggctgtt aaaggcatcg
    45361 ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat caccgacgtg tgtgcagccg
    45421 tggcgcagtt gcgtgagtca ggctgtgatg gggtgatcgc atttggcggc ggctcggtgc
    45481 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
    45541 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca acgaccgccg
    45601 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
    45661 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat tctcgacgcc gcgttgaccg
    45721 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
    45781 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
    45841 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
    45901 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
    45961 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccacac ggtctggcga
    46021 acgccatgtt gctgccaacg gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
    46081 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcgtgacgct attaacgcgg
    46141 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggtgcgacat
    46201 ctgcgcatta cggcgcatgg gcgcaggccg cgctggaaga tatttgtctg cgcagtaacc
    46261 cgcgtaccgc cagcctggaa cagattgtcg gactgtacgc ggcggcgcaa taaatgccgg
    46321 atgcgacgct tgccgcgtct tatccagcct acgggattgc acatgtaggg cggataaggc
    46381 gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgatgct tgccgcgtct
    46441 tatccggcct acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    46501 tggcgcggtg caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc
    46561 acatgtaggg cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga
    46621 gtaaaggcga tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata
    46681 gctgccgtag acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc
    46741 ggtaaaagta tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct
    46801 ctgggtctgg cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg
    46861 ccggtaatta tcccggttta cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg
    46921 ctgctggcgt gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta
    46981 gcagcgtggc tatactctgg gttaattctt gggtcgatga tggggccaac gattgtgttt
    47041 tccattccgg tggcgctcgg cattatcgaa ccttctgacc gtcgttatct ggcgctcggc
    47101 gtgctggcgg gcattgtgac cattccgatt ggctgtattg ctggtggtct ggttgctatg
    47161 tactccggtg tgcagatcaa cggtcagccg gtggaattca ctttcgccct gatcctgatg
    47221 aacatgatcc cggtgatcat tgttgcgatt ctggtggcgc tggggctgaa attcatcccg
    47281 gaaaaaatga tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc
    47341 ggtcttgctg ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac
    47401 cctatcttta tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc
    47461 ggttctatct cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg
    47521 tttgaaaaac cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc
    47581 ggcatggtgg caacgcttgc caacaacatc ccgatgttcg gcatgatgaa gcagatggat
    47641 acccgcggca aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac
    47701 cacttaggct tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag
    47761 ttgatcggcg gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg
    47821 accacggcta aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg
    47881 gtatcgatat cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc
    47941 gtgcggcggt gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa
    48001 gcccggtgtt ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaaa
    48061 ccttaatcct cgagcaatat caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg
    48121 ccatcatcat caccggtgaa agcgcgaaaa cccgcaatgc tcgtccggcg gtgatggtgc
    48181 tctcccagtc gctgggcgat tttgtcgttg ccagcgccgg gccgcacctc gaatccgtca
    48241 ttgccggtca cggtgccggg gcacaaaccc tttctgaaca acggctgtgt cgggtactga
    48301 atattgacat cggcggtggc actgcgaact acgccctgtt cgatgccgga aaaatcagcg
    48361 gcactgcctg cctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg
    48421 tttacgctca taaaccgggg cagatgattg tggatgagtg cttcggcgca ggcaccgacg
    48481 cccgttcgct gaccggcgcg cagctggtgc aggttacccg gcggatggca gaactgattg
    48541 tcgaagtgat tgacggaacg ctctcgccgc tcgcgcaggc attgatgcaa accggtttgc
    48601 tgcccgcagg tgttacgccc gaaatcatta cgctttctgg cggcgtgggc gaatgttatc
    48661 gccaccagcc cgccgacccg ttctgttttg ccgatattgg cccgctgctg gcaacggcgc
    48721 tgcatgacca tccgcgcctg cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg
    48781 ccacggtgat tggcgcgggc gcgcataccc tttcgctctc tggcagcaca atctggcttg
    48841 agggcgtaca actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc
    48901 tggtgagtgc ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt
    48961 acgtgctggc gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca
    49021 acgcgctggt cgatttcgtc gcgcgttttc caaatccgca tcccctgctg gtggtggccg
    49081 ggcaggactt tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt
    49141 tggcagtcat tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc
    49201 tttttggcgg atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcctgaggga
    49261 acgacttatg aaactaaaga ccacattgtt cggcaatgta tatcagttta aggatgtaaa
    49321 agaggtgctg gctaaagcca acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc
    49381 ggcaagctca caggagcgcg tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga
    49441 catccgcaat aatccggtga ttgcctatga agatgactgc gtgacgcggc tgattcagga
    49501 cgatgttaac gaaacggcct acaaccagat taaaaacagg agcatcagcg aactgcgtga
    49561 gtatgtgctg agcgatgaaa ccagcgtgga cgacattgcc tttacccgca aagggctgac
    49621 ctcggaagtg gtcgcggcgg tagcgaagat ttgctccaac gcggacctga tctacggcgc
    49681 gaagaaaatg ccggtaatca aaaaggccaa taccaccatc ggtattccgg gcaccttcag
    49741 cgcccgtttg cagccaaatg acacccgtga cgacgtgcaa agtatcgctg cacaaatcta
    49801 cgaagggctt tccttcgggg tgggcgatgc ggtgatcggc gttaacccgg taactgacga
    49861 cgtggaaaac ttaagccgcg tgttggacac catttacggc gtgatcgaca aattcaacat
    49921 cccaactcag ggctgcgtac tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg
    49981 cgcgcctggc ggactgattt tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt
    50041 tggcgtggaa ctggcgatgc tcgacgaagc gcgcgcagtg ggtgctgagt tcaaccgtat
    50101 cgccggggaa aactgcctct acttcgaaac cgggcaaggt tcggcgctgt ctgctggcgc
    50161 taacttcggc gcagaccagg tgacgatgga agcacgtaac tatgggctgg cgcgtcatta
    50221 cgatccgttt atcgtcaaca ccgtggtcgg ctttattggg ccggagtatc tctacaacga
    50281 ccgccagatt atccgtgctg gcttagaaga tcactttatg ggcaagctga gcggcatctc
    50341 tatgggctgt gactgctgtt acaccaacca cgctgacgct gaccagaacc tcaacgaaaa
    50401 cctgatgatc ctgctcgcca ccgcaggctg caactacatc atggggatgc cgctgggcga
    50461 tgacatcatg ctcaactatc agaccaccgc attccacgac actgccactg tgcgtcagtt
    50521 actcaacctg cgcccgtcac cggagtttga acgctggctg gaaagcatgg gcattatggc
    50581 aaacggtcgc ctgaccaaac gggcgggcga tccgtcactg ttcttctgat gacgcggaga
    50641 taacacatca tggatcaaaa acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg
    50701 ggacaagcgg cccccgcgcc gtcagaagca aagtgcgcca ccactaactg tgcggcaccg
    50761 gtgacctcgg aaagctgcgc gctggattta ggttctgcgg aagcaaaagc gtggattggt
    50821 gttgaaaacc cgcatcgcgc agacgtatta acagaactgc gccgcagcac cgtggcccgc
    50881 gtttgtaccg gtcgtaccgg tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc
    50941 gatcactctc gttcgaaaga taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg
    51001 cagggcttgc tggaagtacg ctccgaaatt agcgacaaaa atctctacct gactcgcccg
    51061 gatatgggcc gccgcctgtg tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct
    51121 aatccggacg tacaggtcgt tatttctgat ggcctgtcaa ctgatgccat caccgtgaac
    51181 tacgaagaga tcctgccgcc gctgatggcg ggcctgaaac aggccgggct gaaagtcggc
    51241 acgccattct ttgttcgtta tggtcgcgtg aagattgaag atcagatcgg tgagatcctc
    51301 ggcgcgaaag tggtgatcct gctggtgggg gaacgtccgg ggttgggtca gtcagaaagc
    51361 ctctcctgtt acgccgttta ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc
    51421 tgcatctcta acattcacca gggcggcacg ccgccggttg aagccgcagc cgtcattgtg
    51481 gatttggcga aacgtatgct ggagcagaaa gcatccggca tcaacatgac ccgataagga
    51541 ggcatcatgc cagctttaga tttgattcga ccgtcggtaa ccgccatgcg ggtgattgcc
    51601 tccgttaacg ctgaatttgc gcgtgaacta aaattgccgc cgcatattcg tagcctcggg
    51661 ctgatttctg ctgattccga tgacgtcacc tacattgcgg ctgacgaagc gaccaagcag
    51721 gcgatggtcg aagtggtata tggccgctcg ctgtatgcgg gagcagcaca cggaccgtca
    51781 ccgactgccg gtgaagtgct gattatgctt ggtgggccaa acccggcgga agtgcgtgct
    51841 ggtctggatg cgatggttgc gcatattgaa aatggcgctg ctttccagtg ggctaatgac
    51901 gcagaaaaca ccgcattcct ggcacatgta gtttcgcgta ccggttctta tctttcatca
    51961 accgccggga tcacgcttgg cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg
    52021 acctacggca ttgatgcagc gttgaaatct gccgacgtac agctggtgac ctatgtcccg
    52081 ccaccgtctg aaaccaacta ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa
    52141 gcagcctgta acgcctttac cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt
    52201 gcgtaacgga ggttgccgat gatcaatgca ctgggattgc tggaagtgga cggcatggtc
    52261 gccgcgatag atgcagcgga tgccatgctc aaagcagcta acgttcgtct gctcagtcac
    52321 gaagtgcttg accctggtcg cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt
    52381 gcggcgctgg acgcaggttg tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag
    52441 gagatcggtc ggccagacga tgacacccag tggctggtta ctggctttaa ccgccagccg
    52501 aagcaacccg taaaggaacc cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg
    52561 ttggcgctgt taacatcagt acgtcaggga atgacggcag gagaagtggc tgcccacttt
    52621 ggctggccgc tggaaaaagc cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta
    52681 cgtaaacgca gtagtcgtta tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt
    52741 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52801 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52861 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52921 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52981 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    53041 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    53101 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    53161 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    53221 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    53281 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    53341 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    53401 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53461 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53521 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53581 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53641 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53701 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53761 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53821 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53881 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53941 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    54001 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    54061 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    54121 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    54181 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    54241 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    54301 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    54361 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54421 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54481 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54541 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54601 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54661 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54721 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54781 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54841 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54901 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54961 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    55021 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    55081 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    55141 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    55201 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    55261 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    55321 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    55381 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55441 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55501 gcttgcgaca ttcctggcaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55561 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55621 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55681 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55741 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55801 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55861 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55921 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55981 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    56041 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    56101 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    56161 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    56221 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    56281 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    56341 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    56401 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56461 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56521 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56581 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56641 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56701 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56761 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56821 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56881 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56941 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    57001 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    57061 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    57121 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    57181 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    57241 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    57301 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    57361 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57421 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57481 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57541 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57601 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57661 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57721 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57781 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57841 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57901 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57961 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    58021 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    58081 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    58141 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    58201 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    58261 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    58321 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    58381 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58441 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58501 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58561 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58621 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58681 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58741 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58801 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58861 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58921 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58981 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    59041 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    59101 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    59161 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    59221 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    59281 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    59341 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    59401 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59461 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59521 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59581 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59641 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59701 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59761 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59821 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59881 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59941 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    60001 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    60061 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctggctgt ccattaacgc
    60121 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    60181 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    60241 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    60301 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    60361 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60421 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg
    60481 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60541 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60601 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60661 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60721 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60781 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60841 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60901 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60961 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    61021 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    61081 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    61141 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    61201 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    61261 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    61321 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    61381 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61441 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61501 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61561 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61621 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61681 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61741 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61801 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61861 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61921 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61981 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    62041 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    62101 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    62161 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    62221 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    62281 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    62341 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    62401 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62461 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62521 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62581 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62641 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62701 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62761 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62821 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62881 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62941 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    63001 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    63061 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    63121 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    63181 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    63241 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    63301 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    63361 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63421 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63481 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63541 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63601 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63661 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63721 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63781 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63841 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63901 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63961 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    64021 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    64081 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    64141 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    64201 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    64261 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    64321 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    64381 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64441 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64501 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64561 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64621 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64681 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64741 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64801 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64861 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64921 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64981 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    65041 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    65101 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    65161 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    65221 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    65281 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    65341 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    65401 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65461 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65521 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65581 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65641 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65701 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc
    65761 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65821 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65881 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65941 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    66001 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    66061 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    66121 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    66181 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    66241 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    66301 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    66361 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66421 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66481 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66541 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66601 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66661 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66721 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66781 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66841 taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttcagg
    66901 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66961 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    67021 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    67081 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    67141 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    67201 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    67261 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    67321 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    67381 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67441 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67501 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67561 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67621 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67681 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67741 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67801 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67861 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67921 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67981 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    68041 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    68101 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    68161 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    68221 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    68281 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    68341 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    68401 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68461 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68521 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68581 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68641 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68701 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68761 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68821 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68881 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68941 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    69001 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    69061 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    69121 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    69181 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    69241 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    69301 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    69361 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69421 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69481 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69541 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69601 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69661 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69721 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69781 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69841 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69901 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69961 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt ggtgattacc
    70021 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    70081 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    70141 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    70201 gcggggttgc gcgtactgga agtgatgcgc tacacccagc agcatgagag cgatgaattg
    70261 attttgccgc cgctggcgga agcataaaaa atggcgccga tgg
//
LOCUS       DAZRVY010000006        66724 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZRVY010000006 DAZRVY010000000
VERSION     DAZRVY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.006"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXOX_000372"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXOX_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR7003948.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHXOX_000373"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHXOX_000373"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR7003949.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXOX_000374"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXOX_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR7003950.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXOX_000375"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXOX_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR7003951.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXOX_000376"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXOX_000376"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR7003952.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXOX_000377"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXOX_000377"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR7003953.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHXOX_000378"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHXOX_000378"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR7003954.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXOX_000379"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXOX_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR7003955.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXOX_000380"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXOX_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR7003956.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXOX_000381"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXOX_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR7003957.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXOX_000382"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXOX_000382"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR7003958.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXOX_000383"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXOX_000383"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR7003959.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXOX_000384"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXOX_000384"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR7003960.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXOX_000385"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXOX_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR7003961.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHXOX_000386"
     CDS             complement(14282..14581)
                     /locus_tag="ACHXOX_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR7003962.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHXOX_000387"
     CDS             complement(14793..15233)
                     /locus_tag="ACHXOX_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7003963.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHXOX_000388"
     CDS             15334..15933
                     /locus_tag="ACHXOX_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR7003964.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHXOX_000389"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHXOX_000389"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR7003965.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHXOX_000390"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHXOX_000390"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR7003966.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHXOX_000391"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHXOX_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR7003967.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHXOX_000392"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHXOX_000392"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR7003968.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHXOX_000393"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHXOX_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR7003969.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHXOX_000394"
     CDS             complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHXOX_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR7003970.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21614..22777)
                     /locus_tag="ACHXOX_000395"
     CDS             complement(21614..22777)
                     /locus_tag="ACHXOX_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR7003971.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22777..23256)
                     /locus_tag="ACHXOX_000396"
     CDS             complement(22777..23256)
                     /locus_tag="ACHXOX_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7003972.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23271..25718)
                     /locus_tag="ACHXOX_000397"
     CDS             complement(23271..25718)
                     /locus_tag="ACHXOX_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7003973.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25711..25866)
                     /locus_tag="ACHXOX_000398"
     CDS             complement(25711..25866)
                     /locus_tag="ACHXOX_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR7003974.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25863..26138)
                     /locus_tag="ACHXOX_000399"
     CDS             complement(25863..26138)
                     /locus_tag="ACHXOX_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR7003975.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26195..26713)
                     /locus_tag="ACHXOX_000400"
     CDS             complement(26195..26713)
                     /locus_tag="ACHXOX_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR7003976.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26726..27916)
                     /locus_tag="ACHXOX_000401"
     CDS             complement(26726..27916)
                     /locus_tag="ACHXOX_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR7003977.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28327..29745
                     /locus_tag="ACHXOX_000402"
     CDS             28327..29745
                     /locus_tag="ACHXOX_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7003978.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29860..30387)
                     /locus_tag="ACHXOX_000403"
     CDS             complement(29860..30387)
                     /locus_tag="ACHXOX_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7003979.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30391..32709)
                     /locus_tag="ACHXOX_000404"
     CDS             complement(30391..32709)
                     /locus_tag="ACHXOX_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR7003980.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32720..33250)
                     /locus_tag="ACHXOX_000405"
     CDS             complement(32720..33250)
                     /locus_tag="ACHXOX_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR7003981.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33243..34151)
                     /locus_tag="ACHXOX_000406"
     CDS             complement(33243..34151)
                     /locus_tag="ACHXOX_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR7003982.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34156..34503)
                     /locus_tag="ACHXOX_000407"
     CDS             complement(34156..34503)
                     /locus_tag="ACHXOX_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR7003983.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34500..35135)
                     /locus_tag="ACHXOX_000408"
     CDS             complement(34500..35135)
                     /locus_tag="ACHXOX_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR7003984.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35202..35654)
                     /locus_tag="ACHXOX_000409"
     CDS             complement(35202..35654)
                     /locus_tag="ACHXOX_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR7003985.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35647..36114)
                     /locus_tag="ACHXOX_000410"
     CDS             complement(35647..36114)
                     /locus_tag="ACHXOX_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7003986.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHXOX_000411"
     CDS             complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHXOX_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR7003987.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHXOX_000412"
     CDS             complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHXOX_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR7003988.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36635..37060)
                     /locus_tag="ACHXOX_000413"
     CDS             complement(36635..37060)
                     /locus_tag="ACHXOX_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR7003989.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37075..37572)
                     /locus_tag="ACHXOX_000414"
     CDS             complement(37075..37572)
                     /locus_tag="ACHXOX_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR7003990.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37572..37853)
                     /locus_tag="ACHXOX_000415"
     CDS             complement(37572..37853)
                     /locus_tag="ACHXOX_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR7003991.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37857..38060)
                     /locus_tag="ACHXOX_000416"
     CDS             complement(37857..38060)
                     /locus_tag="ACHXOX_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR7003992.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38060..38569)
                     /locus_tag="ACHXOX_000417"
     CDS             complement(38060..38569)
                     /locus_tag="ACHXOX_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR7003993.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38669..39412)
                     /locus_tag="ACHXOX_000418"
     CDS             complement(38669..39412)
                     /locus_tag="ACHXOX_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR7003994.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39416..40488)
                     /locus_tag="ACHXOX_000419"
                     /pseudo
     CDS             complement(39416..40488)
                     /locus_tag="ACHXOX_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40547..41401)
                     /locus_tag="ACHXOX_000420"
     CDS             complement(40547..41401)
                     /locus_tag="ACHXOX_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR7003995.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41575..43347
                     /locus_tag="ACHXOX_000421"
     CDS             41575..43347
                     /locus_tag="ACHXOX_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR7003996.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43347..44381
                     /locus_tag="ACHXOX_000422"
     CDS             43347..44381
                     /locus_tag="ACHXOX_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7003997.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44753..45895
                     /locus_tag="ACHXOX_000423"
     CDS             44753..45895
                     /locus_tag="ACHXOX_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR7003998.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45897..46577
                     /locus_tag="ACHXOX_000424"
     CDS             45897..46577
                     /locus_tag="ACHXOX_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR7003999.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHLTKGISCGISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTH
                     TKHGEKLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYI
                     NNNEYLEQ"
     gene            complement(46607..47629)
                     /locus_tag="ACHXOX_000425"
     CDS             complement(46607..47629)
                     /locus_tag="ACHXOX_000425"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR7004000.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(47716..50013)
                     /locus_tag="ACHXOX_000426"
     CDS             complement(47716..50013)
                     /locus_tag="ACHXOX_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR7004001.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(50003..50278)
                     /locus_tag="ACHXOX_000427"
     CDS             complement(50003..50278)
                     /locus_tag="ACHXOX_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR7004002.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(50275..50499)
                     /locus_tag="ACHXOX_000428"
     CDS             complement(50275..50499)
                     /locus_tag="ACHXOX_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR7004003.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(50502..50801)
                     /locus_tag="ACHXOX_000429"
     CDS             complement(50502..50801)
                     /locus_tag="ACHXOX_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR7004004.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(50801..51025)
                     /locus_tag="ACHXOX_000430"
     CDS             complement(50801..51025)
                     /locus_tag="ACHXOX_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR7004005.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(51089..51589)
                     /locus_tag="ACHXOX_000431"
     CDS             complement(51089..51589)
                     /locus_tag="ACHXOX_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR7004006.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(51759..52031)
                     /locus_tag="ACHXOX_000432"
     CDS             complement(51759..52031)
                     /locus_tag="ACHXOX_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR7004007.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            52168..52461
                     /locus_tag="ACHXOX_000433"
     CDS             52168..52461
                     /locus_tag="ACHXOX_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7004008.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            52531..53511
                     /locus_tag="ACHXOX_000434"
     CDS             52531..53511
                     /locus_tag="ACHXOX_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7004009.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(53697..54197)
                     /gene="cpxP"
                     /locus_tag="ACHXOX_000435"
     CDS             complement(53697..54197)
                     /gene="cpxP"
                     /locus_tag="ACHXOX_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR7004010.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            54347..55045
                     /gene="cpxR"
                     /locus_tag="ACHXOX_000436"
     CDS             54347..55045
                     /gene="cpxR"
                     /locus_tag="ACHXOX_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR7004011.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            55042..56415
                     /gene="cpxA"
                     /locus_tag="ACHXOX_000437"
     CDS             55042..56415
                     /gene="cpxA"
                     /locus_tag="ACHXOX_000437"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR7004012.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(56521..57195)
                     /gene="yiiM"
                     /locus_tag="ACHXOX_000438"
     CDS             complement(56521..57195)
                     /gene="yiiM"
                     /locus_tag="ACHXOX_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR7004013.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(57344..58327)
                     /gene="kdgT"
                     /locus_tag="ACHXOX_000439"
     CDS             complement(57344..58327)
                     /gene="kdgT"
                     /locus_tag="ACHXOX_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR7004014.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(58587..59207)
                     /gene="sodA"
                     /locus_tag="ACHXOX_000440"
     CDS             complement(58587..59207)
                     /gene="sodA"
                     /locus_tag="ACHXOX_000440"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR7004015.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            59492..60526
                     /gene="rhaT"
                     /locus_tag="ACHXOX_000441"
     CDS             59492..60526
                     /gene="rhaT"
                     /locus_tag="ACHXOX_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR7004016.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(60523..61371)
                     /gene="rhaR"
                     /locus_tag="ACHXOX_000442"
     CDS             complement(60523..61371)
                     /gene="rhaR"
                     /locus_tag="ACHXOX_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR7004017.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(61445..62281)
                     /gene="rhaS"
                     /locus_tag="ACHXOX_000443"
     CDS             complement(61445..62281)
                     /gene="rhaS"
                     /locus_tag="ACHXOX_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR7004018.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            62569..64038
                     /gene="rhaB"
                     /locus_tag="ACHXOX_000444"
     CDS             62569..64038
                     /gene="rhaB"
                     /locus_tag="ACHXOX_000444"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR7004019.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            64035..65294
                     /gene="rhaA"
                     /locus_tag="ACHXOX_000445"
     CDS             64035..65294
                     /gene="rhaA"
                     /locus_tag="ACHXOX_000445"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR7004020.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            65536..66360
                     /gene="rhaD"
                     /locus_tag="ACHXOX_000446"
     CDS             65536..66360
                     /gene="rhaD"
                     /locus_tag="ACHXOX_000446"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR7004021.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            66370..66684
                     /gene="rhaM"
                     /locus_tag="ACHXOX_000447"
     CDS             66370..66684
                     /gene="rhaM"
                     /locus_tag="ACHXOX_000447"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR7004022.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        16083 a        17657 c        16880 g        16104 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tcgaccataa ataagcccct caactgaggg
    21301 gctatttttt tgatcacatc cacataattt gctgccctga cggcaacggg tgcggcctta
    21361 cggcgtggac ttctcccggc ttcacgatgt atcgctgtac cgactcataa gtgatgaacg
    21421 tggcgctgca attcacgttc tggcactggt gataacgctc ttttgtcgtg tcagtgatat
    21481 agcgacttgt acgcgcatgt gcggcatgct ggcataaagg acaatgaaac atcgcgagca
    21541 cctcttccgg ttttgttgat ggtgccattt tagttaattt atccttataa aacaaacaga
    21601 taaataaacc acgtcactca tcatcttctg tttcgtactc cacatcagaa agccttacct
    21661 caagctccaa ggacgtcgtg aagccgctat tattgagaca atgtgtcacc ttagtgatgg
    21721 tccagtcctg ctcgtctatg acgcgcttaa agcctgacac tttaaccggt gtttccgtgt
    21781 aaatatctgc acgaccggta gccaggctga tggagaactc cgctacaccc cgttgcagtt
    21841 tatcccactt cgcctgagcg gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca
    21901 gggcaaaaac gttgtcagct tcaccggcca tgtattcacc ttcgcgcgct tccggtactt
    21961 ttggcgcttt cttctgcgtg accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt
    22021 tttctttctt tttgcgtttc agttttacct tctgcttttg cggcttcggg tctttggtgt
    22081 gtaaccactt tgccgttacg ccggtgtaag ctccacggtc agcaatcgca aaatgatgac
    22141 ggtcgccgtc gctgcgggtg atggtaatct gcgggatttt tttaccgctg gccgtcaccc
    22201 cctgccccgc tttgagaaac agcagttttc ccatttttac cgacacctcg ccgccgttgc
    22261 gttcagcaag acgggtcagg aatttcgcat cagactcctg cgactggtcg atgtgtggaa
    22321 tttttattcc ggccagtgac ggagcgacac tggcttccag cctgttacgg gaggctatcg
    22381 cctcaacaat cgcaccgagc gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg
    22441 tcccgcgaaa atctgcactc cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt
    22501 caacctcatc aacggtaaat ttccctttgc ataccagggc aaaacctttc cagccgatat
    22561 acaccgtcag gacagcgcca cgaaccggca gcccgacctg cccgtcggca tcgttcagtt
    22621 caatatcaag ctggtcagcc tcaaagcccc ggttatccgt cagggtcatg ctcatcagac
    22681 ggtcgctgat attgccggta atatccctgc tgtcgagcat cagcatgtaa tccggcgtca
    22741 gcgtactgcc tgcatcaaat gtcagcgcat ccagcattat cccgcccccg tcatacccgt
    22801 gaatttagtc gccatactgc cagccttacc gatgagcgat tccgcctgtt taccgatatc
    22861 gccataaagc gcggccagtg actcatcaac gcgggtgagc gacagcgtaa aatcaatttt
    22921 ccgtggtgtg ccgtctgcaa agaaaatact ccctgtttca ctcaccttgc tgatgacata
    22981 catgccgtaa atcatgccgg tgccatccag caacggccac gcccggcctt cctctgccat
    23041 cagtctgagc gtggtcatcg tcagctttcc gccagtcagt tcgggataaa gcacaccggc
    23101 aagtgtgatg ttctcctcac ctacaccgag aaactgatag gcatcccgtt taccgatacg
    23161 ggaatttgac ggccagcgat aatctgattc acgctgcatg gtctggtgtg gcagcgtctg
    23221 gcgcataaaa acaaacatac ctaacgcgag catcattttt cgtcacctcc ttaaccgtca
    23281 tgcatcatgc tggcacgggc gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc
    23341 tgtaactggc ggtcaagctg tgtccccggt gcagtaccac ccgtcaggct gatgtgatat
    23401 tcgtttttac tctggtccac ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc
    23461 tgatagcctg cataagagct ggtcgccgga atataaccac cggtgccata cgtggcggca
    23521 tgagttctgg cggcggtctg gtcaagtgtg tctgactctt tgttgatgac cccgagcttt
    23581 tccagtaccc agtcaatgcc gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc
    23641 gcgtcagcca gtgcctgccc gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc
    23701 tgggtggctt tgaccggggc aatcaggttt ttaaaccact gccacgcagc ctgtaacttt
    23761 tcgcccagcc agtcaaacac cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat
    23821 gccgctttca gcccttcaac cacaccgcca aagaatgcgc tgacaggctc ccagtattta
    23881 cggataagca acgccccggc gacaatggcg gccaccacag ccacaaccgg ccagctaatc
    23941 gccccgatgg ccgtcataac ggcactacca accgtcgtga agattgcccc cattgcgcct
    24001 gctgccgcga tgatggcatt gatgccggtg ataaccggcc aggctacaag accaattgca
    24061 ccgatgatgc cagtcagcgc cagcgcgcca ccggcaatga ggccaatggt tgacgccagt
    24121 gatttgtttt tctgtaccca gccgtcgagt tttaacacat actttgtggc cgtctgcgtg
    24181 agcttacgta gcgcgccttc ctgctggtca aacaggtctg tccccaccgc ctcataagcg
    24241 gactgaaact ccttaaagtc accgccgagg ttgtcctgca tgatatttac cagctctgcg
    24301 gtcttcccgt ctgaggcttt aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg
    24361 gcagtcatca gcacagcggc ggctgagctg gcctcctccc cgaaaatggt tttcatgtat
    24421 tcggcctgct gggcagtacc gagccggttt ttctcaaagc tggcctgcat ttctttcaga
    24481 atggtaaata ctggtcgggt gtttccctta ctgtctgagg ttttcacgcc aagctctttg
    24541 agtgcatccc atgcttttcc agtcggtgcc tgcaggcgac ttaacacggc acggcttccc
    24601 gtccccgcca ttgagcctgt gatttttgca tcatgcagcg ccccgaccat tgcggcggtt
    24661 tcttcaatgc tgacaccggc attttttgcc actggtgcgg cataggtcag cgcatcgctc
    24721 atgccgtcaa aatcagcggc ggttttgttc atcgtcatgg agagaacatc cccgatatga
    24781 gcgaccttat cgtttgaaag ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt
    24841 tcttccattg tgcggcggtt cgccagcgcc atattcagcg tgaccggcgt tgccgcctga
    24901 atggcatcaa catccccacc cgctttcgca atgataatct gtgcaccggc cgcatcatcc
    24961 gctgaggcgg cggtgttgtc accgagctgg cgcgcctgct tgcggagtgc agccatttcg
    25021 gcggagtctt ttgccacacc gagcacagcc tgcaattctg agtttttctg cgcaaactca
    25081 taacctggca tcagcagctt aactccggct atcgttcccg cagcagcaat ccccacaccg
    25141 gcagcgccca ctgaggccat atttccggcc agttcctttc cggcctgata acgctgtttg
    25201 actgcgttaa gttttgcctg ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta
    25261 agctgggcgg tggtttcact gatacggttt ttcagtccct gctcatcatg tgcaagattg
    25321 cgggtattaa ttcccacagc ggccagttcc cgctgctggc gtttaacgga atctgtcagg
    25381 cggttatatt tcgcctgtaa gtcctccgcc gcacgctttg cggattccag cactttcgcc
    25441 tgagcacggg tcgggcgttc ggtgttttta aactgtgtgg caagggcttc ggcctcctgc
    25501 cgtgcctttt caagtgcatg accagtcacg gcgagctgtg cactggtctt gcggaatccc
    25561 tcaatacggg atgcgtgacc gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc
    25621 cccgacagca acttactcgc tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca
    25681 gccctgagca atacctgtaa ttttacattg ttactcattc gtgtttccgc ttcgcctgag
    25741 cgccttttcg cgccatgtga tgagttcggt caggctcatg ggatacagtt ctgatggcgg
    25801 ccagtgaaat atcactgcca catccgccat caggtcatcg accgacagat ttttcggaag
    25861 cgtcactgca ccgagttcgg cgacaaaaaa ccgaccacct taccggccag cgccacaagg
    25921 tcaggcagtt ccagcgcggc gacttcctgc tcggtcagca tcggtgccgt catgcgcggc
    25981 agcaccttaa tcagtgcatc gacttctgag tttgcgaccg cagccagact gacaccgcgc
    26041 agcgtccccg cactgggttt catcagcgtg acctgttcga taacctgctc accacgcttg
    26101 accggattgt ccagggtaat cacattttct ttgttcatgg ttttctcact tctgaatcgg
    26161 ggttaaccgg tcagccaggc tgaccggatg aaaattacag gccgatattg cggcggtgtt
    26221 gctccagccg gtcgacgccg ttcaccttct caatcatgtt gatggtgtcg atttcgacca
    26281 gctccttacc gtccatcgtc agccggaaat aggtgcagac cacggagatt ttcgactcgg
    26341 tgtcttctcc ctgtttaccc tcgccggtgt cgatttcttt ctgacggcca cgcatgacca
    26401 cctctacggc caccgtttcg ccggtatcgt cacgctggta agagcctgca aaacgaatcg
    26461 gcacggcatc cacaccggtt gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc
    26521 cgagcgacca ctccattgac agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc
    26581 cgttcatccc cgcaccgcga tagttttcga gtttacgggt cagttttggc agcgtgacgg
    26641 actttgcaac gccctgatag ctgtagccgt tcagaaagac gttcattaac ttgagtttgc
    26701 gcggcattgc catcggtcag gctccttaat tgctgttaac cgaggtgacc agatttgcca
    26761 ggtatttatc ggtaatacgc tggcgcaggg tcaggttttc aagaggaggc accggggtat
    26821 agtcgtagtc gatatacagt tttccggcct tgagggtttc cgcatcgttg gattcttcgc
    26881 tgaaccagca ggtcgcatcc acgatatagc cgtttgtttt cagctcacgg aatttggcat
    26941 tgatgccgtc aacgatgtcg cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca
    27001 tgtgcgcctc agccatcgtg tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga
    27061 ggaacagcgg gtcatcagag caggtacggt taccccagaa gcggaaaccg tcacggcgaa
    27121 tcagcgttgt gacgcctgac tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat
    27181 cccagaatac ggacgcgctg atgccggtaa caccgttcac cccgacgttg gacagcgttt
    27241 tatgccagcc ctgctcctgg tcgattttag cgcgcagacc cagcgcacgg gcggtggcat
    27301 acgcggtggc ggtggtactg gcgaccgtat cccatgcgag gaaatccggc cagatgacca
    27361 tcagctcacg ctggctgaaa ttctgacggt aggctttcac ctcggaaacg gtcttacagc
    27421 cccatgcgct gatatacccg aaagcgcgca gcttctgaca gactgatgcc agtgcaacag
    27481 ccacctcttt ggtgtccagt cccggcacgc cgagaatacg cggtttaaca ccggttaccg
    27541 actccgccgc cagcagggct ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga
    27601 tgatattgga aacggtctgc gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca
    27661 cacgcacgac aacggtgacc ggttttgact ggtcggcgat ggcctgcaac gatgccgcca
    27721 gcgtgccttt tttaccggcc tttgcaattg cgctctgcac attggtaatc agcaccggtt
    27781 tattgagggg gaaggtttcc gcatccgcat cgctggccgt gcagaccatg ccgacaatgg
    27841 cagtggatac ggtggaaatg acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt
    27901 gatgatagtc actcatccgt ttaactccgt ggttaatggg tgcaactatt ttctgttggg
    27961 cagtgcatga gacgctattt gacctggctg gtcagtggat gaaacaacag ataaagaaaa
    28021 tgcgggcaat tcgcccgcat gtcctgattt gtacacactc attttccgac tgacagttta
    28081 catagcccaa acgctatcaa atctgacagc ctgctatgag cgaaaagcgt taaatctcac
    28141 gatatctgct accgggaaaa atttcgatta tcttcgatgg gaactttgat aatgtagtaa
    28201 atgccatttt cagcgatcac ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac
    28261 ttcactgaaa taaggttgct agtatttttg gagcaaccat taatgttttt tatttaagga
    28321 atgtaaatga catacgcact gattgataat tcaactctaa ctgcagtcca aagaatcgag
    28381 gggaaagtaa aaacaagatc gaaggactca gtagatacag acataatagc atttgagaac
    28441 tatatacagt ctattttgtt ctatgatcac gttatcgcgg ttgatgatta tattccagag
    28501 taccgtggga gtaggatttc aagtttcaga gagatattat ttcttgataa agcacaatat
    28561 gggttggatg aaattgaaag tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt
    28621 cgtggtggaa catttgttaa tgacgatttt aaaaaactaa ttgaacttct tcagacacat
    28681 atcatatgta catgggacat tagttcaagt gtttatcatc taacactgaa gaacttatct
    28741 gatggaggta aggagtttga aaaatatggt aacattgctg ctgccatttt ttcagaactg
    28801 cgtgatgctg ctgagtctgg taatagaaca aatggcgatg ttgaacttct cgatcgattt
    28861 ggcaacccta ttcgcaaggg gtacaaagtt cccggagcaa aatgggggga tggcacatct
    28921 gctggtgaag catcagatgc aatcaaagca ttcgtggctt cgcttgtatg gctggctaat
    28981 cgggcagtat tttattctct cacagctaag taccttcaag cagatacttt tttatatcca
    29041 ttacgtcagg cgtatcaaca gacatattta agccaaaact gtcagtatgg ttttgactat
    29101 gctaaacata ttgtagaaaa cttttccact tcgttaagtc atgatctcat tgatattcat
    29161 tctggtggta aagctgtagc aacagcaact tcgcttcctt tattttcggc atggctcgca
    29221 aaagaaactg gtgatccaag ttgtattgtt gaagctgctt ataacataag ggacaacaac
    29281 gaatttgtaa gggcccgtga gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa
    29341 ttgcctcaag ctaataagtc agtaagagca ttgcttactg aagtatcgaa agaatcagat
    29401 tatattcgaa ctaagtatgg gctcaagact agacaaggtg tacctgtaac taagcttgta
    29461 catgtataca atactgttgc ggctattaat tcccttccga aactaccgac ctttaatttt
    29521 aaagttaaag ttcctgattt cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc
    29581 tatcgaaatt tgactaatga tctttctact gtgtggtcgt taggagaagc tcgagatatt
    29641 cttggaagta aagttgtgat agataatcat gcaagaattt acagtccgaa gcatgaagaa
    29701 ccgcgtttca aaaactctca ttccccatat aagagcccga tgtagcttaa gaaaaagaca
    29761 cgcccacctt gtgcgtctac ttctggcaca gagcggactc tcagattagg ctttactctg
    29821 tgccatagat atgtaagctc acaccagagc tcatacaact tattgcggca tttccggcca
    29881 ttcaggattt gcaggatcca cacgactgac cagaacactg tagcgttccc atgcctccag
    29941 tcgactgcgc tcctcatctg ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat
    30001 cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct gctgcaattc
    30061 ctctgccgta taaattcgtt taatcactgt acagtcctta aacatccagt tccctgaaat
    30121 gtccgcccgt cggttagcag taatatccgg cacttcaaca acacttaatc catccggtct
    30181 gatagctgtc acatcctttt ccacatagcg gataatatta tctttgtcgt acgctatttt
    30241 tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat cttctgaaaa
    30301 aaaccagaca acatcaaagt cctttgtcaa ttgatattgt tcaaccgttt ttggattacc
    30361 tgccgttata tttatcaaat gctgcataaa ttatacctgt gccacgttat accaagtccc
    30421 gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg cagagtttga
    30481 gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct gatgtgtcca
    30541 tgtcatacca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgga tatcccggac
    30601 atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa cagcaaaagt
    30661 gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat cacctaattc
    30721 agccaccaca ggacgacttg agtttcccca caaattgaat gcggcttcct tcgtggatgt
    30781 attgctgctg ctgaccgtga actttttccc gctaccggca cgtactttgg tacttgagac
    30841 aatatcacct gtaacactca ggccatgccc cattgacact ccgccattgg cgttattgat
    30901 agtcagcggt cttaagccgt tccatgtccc aaatttatcg ccagaggccg tcagcattaa
    30961 atatgtgctg ccgccatcat tcctgataaa gaatccataa ttgccataag caatgcgcag
    31021 accattagca ctgagtgatg taatttcacc tcttgaacgg agaccataag cggagctgag
    31081 tgataattct tcctgagcgt cattgtttcc tgtcgcccag cgaattaccc cgccctgaac
    31141 tgtttcatgc cagatagtat ctccttctcc accacgaaac tttctgagat attttttgcc
    31201 gcctctggtg cctgaacata aggccgtaga catataggca ttctggcttc cgccatcctg
    31261 attaatcgtt ccggtcattg agtcgccctg acgattccag tcacgacgcc agccggggga
    31321 gtagccgtcc ccatgattta tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga
    31381 tgttgttggc gtggtcactc ggatagtgat tgcagattta gttcccatga cctcgacgac
    31441 acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt tatttgcata
    31501 tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg aatccagcca
    31561 ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc
    31621 acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg caccgttctg
    31681 atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt ttggcgttgc
    31741 ggccagcgtt tcagaatcgc tgttgatggc actacttagc tggacaagac cttttcgcgc
    31801 tgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg tcttaaccgc
    31861 ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg gcgctactga gctggacaag
    31921 accttttcgc gctgtagtgg cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc
    31981 tgtcttaacc gcctttggcg ttgccgcaag cgtttcagac gtgctgttgg tggcactact
    32041 gagctggaca agaccttttc gagctgtggt agcgtcctgt gcggtatatt tcccgttagc
    32101 aaggtcatag gcggccttta ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt
    32161 ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg
    32221 tgactgttcg tgctctgcaa ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt
    32281 ggtgtcaatg gtcagctcca ccgaggccac actgctgacg atgatgacca tgcgacaggt
    32341 ctgcgaacgc cctgagcctt cggcaagagc tggcttataa ctttcggcca tgttcgccac
    32401 ggcaattaac gttcccgcat catcgtacag gccaagctca cgcatccaga aaccgcccac
    32461 ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt cctggctgat
    32521 tttgttcagc gtatgtcgcc agacttcgtg gataagcccg gtctgtccgg catccgggac
    32581 aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct tccgccctcc
    32641 cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg ttctgaattt
    32701 tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg tcgtaagcca
    32761 caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca ataagatgac
    32821 ggcttgcagg tttggcatca gcaataagcc gttccatttc ctgatacatt gcctctgtga
    32881 tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc
    32941 accactccgt cacgttgatg agatagccga gcggctccac cacacgccgg attgcgccga
    33001 tagtgccttt atgacagtgg atgaaatagg catcgcggat aacggcgcgt tttgtcgctt
    33061 ccggccactt ttcatcccac ctgtcgaccg aaaacgccca cgccagccac ggcagcagat
    33121 ttgccggaca ggtatccggg ttccacagtt cacgaatcct gaccggcgtt ttttcaattt
    33181 ccgcacaggc ttttgcggcg gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg
    33241 aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta
    33301 ctgttgagca cgatgtcggc cagcggtgca gtcagttcga cacgctgcac gccttccaca
    33361 tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg
    33421 atgtacgctt ccagtttttt cacggcagca gcgcgtatgg gttcgctttc gggaccaggg
    33481 taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg cggcagactg cacggtcacg
    33541 cggtcggcca ccggcctgac gtcctcacca ttaagggcgt tacgcaccac cgccagcagg
    33601 tcttcggatg cgacaccgtt attttcacgt gacagcacag agatggtgac gcaggccgga
    33661 gacggactgg ttacagaaat atccgcgaca cgcccgtcgg cactgcgacc atgatactga
    33721 taggccccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat acgcagacga
    33781 taatccgtgt cagattccat cactgccggt gtcggcggga gggtcgaatc atctgccggg
    33841 gtgataatca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc attaccggcg
    33901 gcacaggcca gcattaccgc ccgtgcggcc tcattcacac gctgacgcca gataagctca
    33961 cgataagcat tttcctccag cagtttgacg agaggctcgg attccagcgt cagggtacgg
    34021 gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca
    34081 agaatggttt caaagtccag ctcctcgacc acatccggtg cgggtagctg gttcaggtcg
    34141 ataatcggca tggtttcaac tcacagggat ggttaacgaa agtggctggc cggtgtcgtt
    34201 gtgctggccg gttaacgtga ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga
    34261 tgacagggtg acgcgggctt cccatttcag cacggccatg taacaggcga ccttaatctg
    34321 caactcaagc gccggggtct gcggctggtc aatcattgac gccagcaacg agccgtaatc
    34381 acgacgcatc acccgtgagc cgaccggcgt gcgcaggata tcgccaatac tctggctgat
    34441 atgctcaagg tcagagacag tcaggccatc actgcgattc attccgagat aacgcgctgt
    34501 catagaggac tcccggttgt gccgccgctg tcgccggggt gtttatgggt atgcagtacc
    34561 ttcccgtttg atgagagttc accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc
    34621 ttctgcactt ccagcgtgcc agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc
    34681 agagtgacgc gggttgatgc tttcaccgtg accaccggca ccgtggcagt aacagaatca
    34741 gaagccgtca cgctggccgt tttaattccg cttaccgtga gtgcactggt ttcaggttca
    34801 tactcaatca ccgccccgtc agggaaacgg atatgcaggg catccgccga cgcagacggc
    34861 gcggggttat caccggaata aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct
    34921 acggccagaa tcagcacctg ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg
    34981 gcgcgatggg tcagccactg aagccagtcg gtgcacatgc cgccggtctg cacacggcag
    35041 cgaccggcat taaggtcggt ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg
    35101 cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat
    35161 accggcggca atgtgacgat gaggtgtcag gaatggcaca actaacggtc gaggtgagcc
    35221 aggataatct cttcaatcat ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc
    35281 ggataatgaa ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca
    35341 ctggctattt tcggtgactt cccgccgtaa aactccattg atgcctgttc agggctggcg
    35401 cggatatgca aaaaacgact ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg
    35461 gtcttttttc tggcgctctg ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc
    35521 tgtgccatca cccgacgctg ctgactctga cgcagacgtt tcgccagttc ggcgctcagt
    35581 cgccgacgcc ctgacggtga cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc
    35641 ttaaactcat tcatcccact tactcaccag ttcgccattg atataaagct ccatcgggcg
    35701 ggtgaccggc tccggcggcg gaggttccgg gatattcttc acatgcagcg cgccgtccac
    35761 ctcactgacc agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc
    35821 attgttgatg tctgcataaa acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat
    35881 gtcgggctga ttttcccgca gccacgccag caccggcacg atgagcaggt caaaatcacc
    35941 ggtaaagtcg gtcacaatca cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc
    36001 cagtgtggag gcaatactcc cgttatccac gaatatccgc agcatctcgg ggcttgtttt
    36061 cagcaccgtg acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt
    36121 cgtcctgaca gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa
    36181 gctgccggat atcagcactt aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc
    36241 aaaggctcac tttcgggcag gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc
    36301 tggtgcaacc ggcgcacagc atcaggcagg tcagcaccgt accagcggcg aaaatcttcg
    36361 ttttcattaa gtaacctcgt gatggttttc tcgcgctgag cttcacgctt cgcggcgttc
    36421 tccagttcct gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagggca
    36481 acatgaagct ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc
    36541 gccctgtcca gcaaggcgcg caggctggca tttttgtgtt tcaccagaaa cagaccggcc
    36601 accgccagcg ataacaacac aaccagcaca atcatcagtt ttgacatggt tcccgcccct
    36661 caagacgctg acggcaggcc gtacgtatca gccggaaaaa cagcgatgcc acgagataaa
    36721 tcagtgcggt aaaaatccac ccggcagcga ccagcgagat aaacgtcgcc accatcacca
    36781 tcagagccgc tgcccgtctg cgccacggca ccggctgcaa aaacagcgac gtgacaatct
    36841 tcacggccag cgattccggc ggcagctccc gcccgtagcg ttccagcaca tactccgcgg
    36901 catacacgcc gacaccaccg gcaatcacac agataaccgt cgccagaatc gcccatgcag
    36961 cgacaaaact gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca
    37021 gcgacacgtt cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc
    37081 cggtacgccg ccagcggtgc gccagacggt gaccagtttt tccagtgaat gctcacgctg
    37141 accgtaaccg gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc
    37201 aatgcgtccc gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag
    37261 cctgtcctgt gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca
    37321 gaacaggtaa agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa
    37381 caccttcgcc ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg
    37441 cttcccgtcc agtccggtga ctatcacgtc atagccccgg tttttcgtca gcggatgatt
    37501 cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct
    37561 aattaccggc attacggttt ccccctgccc ttaaagcggc gctgaatggc aatctcaatc
    37621 acctgataac cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg
    37681 tcaggaaact gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg
    37741 cgcccgataa acagacgcgg agtgatgggt tcaccaccgg caagcacctt gccgacaaca
    37801 atcagcaccc caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac
    37861 tcccacagat tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg
    37921 gtgccgtgtg gcagcaccgc ccccagttca gccagtcccg gatttgcggc gagcacggcc
    37981 tcgaccacgc cctcagtgcg cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc
    38041 tgtagcgcaa aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac
    38101 gcgccaccgc ccagcgcata tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg
    38161 ccttcttgtc gcccttggca ctggcatcca cgccgcggta acgctcataa agcgacgcgg
    38221 tcgccatcgc acacacagcg cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt
    38281 cgtccgccgg gacgtcagcc agacgcgaaa aaccggcagc aattttctgt tcgcggtact
    38341 cgtacagctc cgcattcgtc tccgccatgc ctgacttgat ggcctcacgc agacgggcgg
    38401 gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg cttcgggtca atatcgggaa
    38461 aaaagaacgt gtttttaatc accggctcgt cgcctgccgg ttgcgggata accaccgtac
    38521 cctcaccgga cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg
    38581 aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg
    38641 ccgccctggc gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt
    38701 ttgccggtgt cacgctccgg gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct
    38761 ccggcttcgg tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac
    38821 agtcgagctg tgtcgcacgt tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac
    38881 gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg
    38941 tggcggtcag ttcgagggtg tccgtcagca ggcggggatc cacagactca ccggcagcgt
    39001 gagcgcgcat ggccgcaagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt
    39061 gtttacccgg catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg
    39121 caatatcgcc ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac
    39181 ctttgccctg ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgtt
    39241 ttttcgcggc cttcagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca
    39301 gcttgaccag catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg
    39361 cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg
    39421 ctcccggctc tgcggtcgct ttagccggtg tggagaaatc accgacctta attttttcca
    39481 ccagacaacc ggcggcgtag tcctccacca cgtaatcaat gttcattgac tcgtagttct
    39541 ccacgcggtc gagtttcggg ttttccacaa tcacgcggcg atggctgtca tccatgtagt
    39601 agatggacag gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc
    39661 gtaccgccgg caggttaccg atgcgtttct ggctgatgat gacgtcagcg gccagcatct
    39721 cgctgttatc ctgctccttg ttgacgatag ggaaatactt gtccgccagt agctgacgcc
    39781 ccacaatcac cacaaggtca gggtcttcct gataccaagg ttcaatcagg ttgttggtcg
    39841 catccatcac cagtgcatcg aggcttacat aatcaccgcc cttacccacg cggataacct
    39901 cagaggtggt gcggccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat
    39961 tcggtatttc tgcagccagc cgaccgccac atcctgcagc atcggattac tgttgcggtc
    40021 agaggtttcg gcacgcttca cgccgttaaa accggccatg atgaaatcaa gggactggcg
    40081 tttgataatg gcgttacgga tacggagctg gaaatcctga taacgcgccc acaggtcaag
    40141 cgttttgtag cggatataaa aatcgaagtt aacctggtcg cattcgtact tgtttgacgc
    40201 cagcttcgag aagtccttcg gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc
    40261 gatggagccg gtgacaccga tgccaatttt ttcccctttc atttcactga ccggcacaat
    40321 gttgatgcgg gtcagaaagt cagaggactc ctgcatggtg ttcatcaggg tctgggtgac
    40381 cgacggttca acggtgaatt ttttcgacac atcaccggcg tcgatgccgt tcagctcggc
    40441 aacacgggac aggtaagcat taaatttaaa gcgggtttcc tggcgcatag tttttcctga
    40501 aattaagggt taatcgtgaa ggttttcccg gactgactga cgccggtcag cagttcgtca
    40561 tcagggcgtc accgccacca ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    40621 tgtggtcgag actgtttttc aggcgggtga atgcctggct ggtttcatcc gccctgtcag
    40681 tcacctcctg cttaagtgca gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg
    40741 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    40801 gacgggcatc atcgctggcc tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg
    40861 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    40921 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    40981 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    41041 aacatttgcc ggtgttggca aagttcggct gaatttccat tgaggtgtag accttctgcg
    41101 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    41161 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcggcc acatcgccat
    41221 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    41281 catagacacg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    41341 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    41401 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    41461 tgcgcatcct ccgccatcag tcccggatgg tttatcactg acacaacagc acattagcga
    41521 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    41581 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    41641 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacggtgcag
    41701 agctggaaac agcgcgacgg ctgggacagt gttgccccca tcagccgtgt cgaaatgagt
    41761 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    41821 gaaattgacc tgctcggacg ccagattgaa cgactggcac gggtaaaccg ctacagccag
    41881 accggcaacg aggcagacct taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc
    41941 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    42001 gagcagtctt tcgactatca gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc
    42061 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    42121 cgcgccctga aaaccggcca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    42181 gtgttccgtg aatacatcat cgcctttgct cgtctggttg acgttgacct gaccggtgac
    42241 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    42301 gcacagagcc ataacggcga cctgtacgtc gacgagattt tctggatccc gaattttcag
    42361 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    42421 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtcgggtga actgttcaac
    42481 cggggacgcg ccagcgccgc cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc
    42541 ggtggtcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgccctg
    42601 aaaggcggct gcacattgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    42661 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag
    42721 gagctgcaac gctgcatggt cgacacgctg gaagaatggg aagactacgc gccgtttgcc
    42781 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    42841 agcgccggat gcgtggtgct ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    42901 gagcgtcacc agtggaaagg catggacttt gccacccagg cggagtccat ccgcaaactc
    42961 accgaaaaat acaacgtcga atacatcggt attgatgcca ccggcctcgg tgtcggcgtg
    43021 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    43081 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    43141 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    43201 ggacgcagtg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    43261 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    43321 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    43381 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    43441 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    43501 ggtatgagcc gccggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    43561 acagctcacc gatttacgtc aaacgtaata ttctggcttc aacgtttatc ccgcacccgt
    43621 ggctttccca gcaggatttc agccgttttg tgctggattt tctggtgttc ggtaatgcgt
    43681 ttctggaaaa gcgttacagc accaccggta aggtcatcag actggaaacc tcaccggcca
    43741 aatatacccg tcgtggcgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    43801 cgacagcctt cgcgcccggc tccgtgtttc acctgctgga gcctgatatt aatcaggagc
    43861 tgtacggcct gccggaatat ctcagcgccc ttaactctgc atggctgaat gagtcggcca
    43921 cgctgttccg ccgcaagtat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    43981 ccgatgccgt gcaggatcgc aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga
    44041 aaggccgcaa taactttaaa aatctgtttc tctatgcccc acaggggaaa gccgacggca
    44101 ttaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    44161 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg gggggcaagc
    44221 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    44281 agcttatccc gttacaggac aggatccgcg agataaacgg ctggctcggt caggaggtca
    44341 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    44401 gcttttttac accccgccat cacgccctca cacgctcatc accgcacaaa aaatcccgca
    44461 gacacaccaa cgcccaacgg gcagactaag cgccgtcacg acgcgctgag acgctggaaa
    44521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    44581 gggcggtttt aatgcagttg catgaacact ccaagagcac gccagcgcta gtgataacta
    44641 acaataaaag cgacttctga cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc
    44701 tatgcggtat gctatccgct tcaattttta acacttgtta agggaactag acatggacat
    44761 tattgattta aaatctcaac tagaaaatga agataatgcc aagaaaagag cagagttgct
    44821 tgctcagcta gaggtgataa agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga
    44881 tgtggatttt gagaccaaag aatttaccgt tgagttatta gttaataagt atcataacgg
    44941 attagaggat gatactaacg aactctttgt tcctgattat caaagggatt ttgtttggag
    45001 tgaaaaaaga caatcaaaat tgatagagtc tttgattttg ggttttccta taccatatat
    45061 atttatagca gatgttctct ccgaagatcc cgatttagat gggcgaattg agattgttga
    45121 tggctcacaa cgagttagga ctatacacgc ttttatccaa aatgaattag ttttacaaga
    45181 cctgaaatct ttagacgtcc ttaatggttt caaattccaa gatttaccat tatcacgtca
    45241 acgtcgtttc atgagaattc ctgtccgcgt tatagaattg agttctaaat gcaatgaaga
    45301 aacccgtaga gatctttttg agcgtattaa ctctggcagc gatatattga aagatatgga
    45361 agtccgtaaa ggatcagaac ttggttcaac aaatttatat acacaggtta ttaagccatg
    45421 ctcaacaata cctaaattta aagaattagc acctttatct cagactaaag aaaaacgaga
    45481 tgaacggtta gaatttacat taagattctt tgcctacgta gataagtaca agaactttga
    45541 tcattccgta cgggattttc taaatgacta tatgtcagaa aatcgagata tagacgaaac
    45601 taaacaaaac tcaatgaaac aagagtttgc taatgttcta tcttttgttg aaaactattt
    45661 cccagcagga ttcaagaaaa ctattactgc aaaatctaca cctcgagtta gattcgaatc
    45721 tctcgctgtt ggcgcagcac ttgcattacg tgaagtccct gaccttgttc ccaagaactt
    45781 agattggcta agctcagaag aattcaaacg attgaccact tccgacggag ctaatagtcg
    45841 cgtgaaggta attgaacgaa ttgaatatgt tcgggataaa ttattagagg attaacatgg
    45901 atcgttttcg tgaggatttt gacgaaaggt caggagagat cttggcctat ttggatcttt
    45961 tgaaatttat tgagtatgcc ggtgcagaac tgatatcagc tgatgataaa gaacataagt
    46021 tctccattac tgctcaatca cgaaaaactc taaagggagc agtatatata ctgttatata
    46081 acctcattga atcaacaatg agagaagcta tctgccttat tcatgagact atttatgata
    46141 gaaacgtaga atttgacaaa ctcaggaaaa atctcaggtc agaaatatta aaaagattaa
    46201 aaaatgaatc tgtaaatgtt gaaagtttag ttaatcacct taccaaaggt atatcttgcg
    46261 gtatatctta tggcacgttc aacaaaaaga aaatattctc aggaaatatt gatagagagg
    46321 aaatcaaaga aaaatcgaaa atatatggtt tttctacaca ttccgattac actcacacta
    46381 aacatggtga aaaattagcc actgttaaac aacatcgtaa tgatttatct catggcaatg
    46441 tatcatttgc tgagattgga aaaaatgttt catatcagga cttagagaat atttcattag
    46501 aagtaatcgc ctatcttgat gctatcgtga ataatataga gcactatata aataataatg
    46561 aatatttaga acaatagtta taaggcctgt aaagcaggcc attttatcat tctaaatggt
    46621 tcttgattga ttgcccaatt gcttttccta gttccacagg aacagcatta cctatcattt
    46681 tcccaatact tcttataaca aacttgtttt tgtcttctac aaaaatataa tccatcggga
    46741 aagtttgcaa taaagcagcc tctctaagag aaatcgctct atgctgttct ggatgtccaa
    46801 aacgcccatt cccgaaacca taacaaagag ttgtaattgt tggacttggc ttatcccaag
    46861 acatgcgccc atatacacta gcataccctt taccgctgga tttcatatga caagcggcaa
    46921 ccaactcttt cggccagtcc ttccatgtac caccaggaac agagtgtata attctttttt
    46981 tattaatcgg atttaattta ctggctctat gtaatatatc atttggatct gtttggccat
    47041 cttctaatgg aggaagattt cctattgcat ctctaactgt tttcaaaata acatcgtcac
    47101 gttttactaa ctcaatcgcc cccaattttg aggccaacaa tacatgacgg gaacggtttt
    47161 gtgggattcc atattcgaca caatcaatct tagacgccca aacataatag cccaacttta
    47221 acaatgaatt ataaaaatca ttataaactt tatgtttagt tacatcagga acgttctcca
    47281 ttgttacgat ttctggagat acttcgcgaa ttaaacgttc gaactcataa agaagaggcc
    47341 attttttatc atcagcctta tcttttcctt gagtatattt tgaaaaaggt tggcaaggtg
    47401 cgcatcctgc caatactttt acagacgcat caccataaag cctcagcagt tcctcttttg
    47461 ttactttagc gatatcttgc tcaataaact tagatttatt gtttctttca tagggaaacc
    47521 tacattcccc ctcaaggtca atgcctgcaa cgacatcaag ccccgcaagc tgcaaaccat
    47581 gagttaatcc gccagcacca caaaaaaggt ctatcacctt tacagtcatc actcactcca
    47641 aatctatacg atactcagta tacatccctt tctacaaaat ctcgaggcct aacttataaa
    47701 ctcatattta ttgagttaac gcttcactga gtttgactct aatactgttg agaatcccga
    47761 ccactcatca gcgagcgggt aagtgaattt tttcccgtca taatttacgg ttgccccacg
    47821 cgccagcacc tcaagctccc atcgctgcgg cctgataccg ttctgagcaa ggtcaacgcg
    47881 gatacgggta atttgcattc gttccgaccg ggtcagtctg gccgatggcg ctatttcatg
    47941 cggttttaac gggcttccgt ttctttgctg acggtttggt cttctcagtc cgtgttttaa
    48001 tgcacctctg agcgccctca caacctccgg gtcattccat tcgataacac cgtcatctac
    48061 cagattaagc actgctgcgg cgtgctcaga aggtgtggga gccggtaacg aagtatcacc
    48121 accagtgagc tttccacagt tattgacagg actccgaggc gcggcgatgc cgctttttaa
    48181 agtcaaaggc tcaacgaccg gcactttcgg aacaatgcgc cagtccgtcg ttctggtgat
    48241 atgaatatga cgcgcgccga gatgcggcgc gtaaatgccg accactctct cgacttcttc
    48301 ctcgtactcg ttaacttcat ccgacggact acgggcgacc ctgacagtct gacaatcgcg
    48361 cgggacattt gccccaccct gcgcgctgat atacagcgca aaatcaccac tgtctgcggc
    48421 ggcgcgtgca gcctcgacgc gctcgtcaaa ctcatcagca atgctgacgc cgcgaggcaa
    48481 tttgcgtagt tcacggtaag cccccattgt cggcaggcca accgttttaa attgcggaat
    48541 gcgccacgtt gacgcccatg cggtaacagc cgcggcagta tctttcagcg gtctgccgct
    48601 atcgttatcg agctgcccat ccagtgcata gccgtcgata ttttttgaga tgtatttcgc
    48661 gatatacccc gcagcaccgc cccgattaag atgttttgcc tgaaaacggt ttcgcgcagc
    48721 tcctctttcg tcgccatcct ctttgagcgc gtagcgacgc atgatttcga taatctggtt
    48781 acgctggcgt ggattacaaa aaagcatcat atgccagtgc ggcgttccgt cgtggtgtgg
    48841 ctcgacgact cgcaaaccgt agacctgtaa atcattatcc ttgaatgccg tgcgcatcag
    48901 gctccagata cggcagagat aacgctgcgc atcctttgga ttaaatgcct catcgttcca
    48961 gccgtgattt agctggacgg ttttactttc gccttttcca acctgacgtg tcgggtgata
    49021 ctttgacggc gcggtcagcg tgataaacat ccccacatca ccttctgcgg cggcgtaacg
    49081 ctcaataccg gcaatggtgt tcatcagctc catccggcga atttcaggat tagaaatact
    49141 gcccatcacc ttactgataa ggtcgatgcg ctcgcctgtt tccctgtttt cgagatcaca
    49201 cgatttaaga aattccagat ttgcctggcg gcgtgcacgc acatcacgaa tggcatgttt
    49261 actggcataa ggagaacggt ctttattgac ctccccgaca gcaatcagta acgcctcatg
    49321 ccagcgcata cgctggcctt taagctgact aatccaccac tcatcgttaa acagacgggc
    49381 aatggcagaa tatgcctgcc tcgtggtcat ctgtccttta cggtattttt tccagtagag
    49441 aggggaaata ttgaaagcac gtgcagcgcc agcaacatga ccatagaggt gagcctgcgc
    49501 ctcatccgta aacagcgatt ctttttcgcc atgcgcatcc acccaggcat cgctgagttc
    49561 ctcatacatc atgaaaagct gcgatgagat acgggcggca aactttttca gctccttgtc
    49621 attcattccc ggcaggcgcg catagtggtc acgctcagcc agaaacagca tcgacgcgtc
    49681 ggtgttcatt tcatggcgct gattcacacg ctcaatgcgc ggccataaac gacgctgaaa
    49741 agtggatgtg aggaaataaa acccgtgcac cgggctttta ttgcgccgga tgtagtcata
    49801 gcgtgacgta aacagcgagc gcaaaaagta aggcaggcgg ttaatcgtgg ataaaacacc
    49861 ttgcacctga cgcatctcgt cacgtgtaag gggtctttcg cgcccgacag cctcgcgtgg
    49921 cgcgttccat gcataagcac cggtaaacgc cttaccggtg cctgcagcaa atgctgacgg
    49981 agggacaaaa cgcccggagg ctttaacggc catatgagcc aaaagcctct gaacaacgct
    50041 tgctgagttg ctcaacctgc gcgtttaaat cagcaaaaga ctttgcgctt ccggtcagaa
    50101 tatcgtgatg catcaggccg gaaacgagct ggcttaattt cgggtaataa ccaaccaccg
    50161 ccagccattc ctgaccggcg tttttaccgc tttccgctct ctttttctcg tggagaataa
    50221 actggaagct gtcactggta acgacataac gttcgccaat ttcaatacga atactcatgc
    50281 cgttctccgg taatgtttgt tttttgcttc aaagactgac tgacaggaaa cacaacgcgt
    50341 ggctgacgga taagccgcac gacgggcagc aggaattggc gcgtcacact cttcgcaaac
    50401 cagcgcagaa gcaccgcaat gttttaccct tgccgcgtta atctggcgct ccagtaattc
    50461 agcctgttgt tcctgaataa aatctatgtt gtccggcatt atcagctcct tttatcgtta
    50521 agtttcctgg atacatcagc gcaataactg gcaagttctg tcgttaattt tgtcagttca
    50581 tccactgagg aaatttgctt gtggaataca gcgcgtttaa caagtaaatt gaccacatca
    50641 gacaggaggt ttaattcatt ctgataaatc gcgataacag attcagtgat gtcgcgtttt
    50701 tctttatcaa gacaaagttg aataagagac aagtcgccat ttttcataac ggcgattttt
    50761 aaggcgttat tcagtaatac aactgaatga gaacaggaca tcaaagcacc tccccgcgag
    50821 acaatccgat attgtgaaat ttttccgact cctgactgag cagctcgact atctccacgc
    50881 gggataactc cgcctttgtg atatggcgaa tcatggcgtc aagatgagaa gaaaagcgcg
    50941 tcgcagcgtc ggcctgtgct tcggttctgg cctgttgcag cagtaatgcg tatttaccgc
    51001 actgattttc agaaactgta tgcatgactt tctccaggca aaaagaagcc ccgcacaatt
    51061 aagtgcgtta aaaactctgg ttaattattt aatgcaaata ttgctctggt tttaccgacg
    51121 tcagaattgt cggtgcatac tcaaacagac tgaatagttc acgtaatgca cggaataaag
    51181 catcacgcca gtaacatgac tcttcattaa ttcgccagta tggctgattg aattcttttt
    51241 cagtcaatcc ggcatgcata aataaagtac gacgctgact gactgttaaa aaactaatat
    51301 atgcatactc acttgcgcca acctgacggc gttttgagaa tgccccacgc aattcatcaa
    51361 ttgcacaaac cagccgttca cgttcgacgt cgttcatttc ttcaaaacgc atcgttgcgt
    51421 gacgttgttt taactgcgca tgaaagcaaa ctgttagccg ttcgcgctcc atcatctgat
    51481 tataataatc acatgtatcc tgccaacgag ggacggcaag atgcttacca attatccggc
    51541 gcatagctgc tggctgtttt tcgacgagat tgagcgtcat cactgtcatt tccagaccct
    51601 ccggcttttc agaaaggtca gagccttttt taacggactc tgttttttgg tgcggataat
    51661 gattcccttg cgacccttcc cgtgggtgat ggtgaagtca atcgccctag ggctttcgtt
    51721 acgcaataac tgagcaatac aacgcggctc attcataatc acaaccccat ccacaaaagc
    51781 catgcatcac gctgttcaac cggtcggtta taaaacgcct cacgtacagc gcgattaaac
    51841 tcaggaatga aaacccattt ttcaccggca cgagccttcg gtttgcaagg atcacgcaat
    51901 tcaataattg gtaatttatt tgccttcacc atttcactga cggctgtctt tggcttccct
    51961 aataaatcag caaatttatc cacatgaacc gcatcaagcg gatactgaat cacataattt
    52021 tcagcgtcca tatatggtac cctcatagga tccagccctt tctaaaccac tcaaaaccgt
    52081 ttagacgctg gtttattctc aaatcaatgg aacctatata ggttccagtt ttgagggaat
    52141 ttagtcccta tataggcacc atgtcaaatg aaattaagcg aaaagattaa ggccttgcgt
    52201 gaggctgaag ggctaagcca atcaaaattc tgtgaaatca tagagttacc gctaagcaca
    52261 cttaaaaaat atgaaggagg aaactttgaa cccggtggca cagctttgct aaaaatcact
    52321 atgcatccca cattccaaaa atatgctcta tggcttatga cagataaaac cgcgccggac
    52381 gcaggacaaa tcgcaccggc tctcgcgcac attgggccag agtcaacaga gtccaaccac
    52441 tccgcgaaaa ggattggcta actctatata aagattacat tttcaccatt tgctaccaag
    52501 atggtgaata cagcgccgga gggctttctt atggcaatta agaagctcga tgatggtcgc
    52561 tatgaagtgg acattagacc tcgcggtcgc gacggaaaac gcatccgcag gaaattcgaa
    52621 agaaaagctg aagcactagc atttgagcga tacacaatcg ccaatgccag tcagaaagaa
    52681 tggggaggcc agcgagcaga ccgccggact ttgagtgagt tgctggacat ctggtggaaa
    52741 tatcacgggc aaaaccacga gcatggaaca aaagagttta atcatctact caaaaccatc
    52801 agcggcatag gtgatatacc agtgagcagg atgagcaaaa gggctttgat ggattatcgt
    52861 tccatgcgac tacgtgatgg tatcagtgcc gcaacgataa accgtgacat gtacagatta
    52921 tccggcatgt tcacaaaatt aattcaattg gatgaatttt ccgggcaaca cccaattcac
    52981 ggactgccgc cactggcgga ggccaaccct gaaatgacgt tcctggaaaa agcagaaatc
    53041 gaaaaactgt taaatgtttt ggctggtgat gacttacttg tcgcgctttt atgtctgagc
    53101 actggaggaa gatggacgaa agttgccacg ctaaaaccag cacagattac aaattgcagg
    53161 gttaccttcc tgaaaaccaa aaacggtaaa aagcgaaccg tgccgatttc tgaggaactg
    53221 gagaaaaaag ttaaagagga ggccagcgcc aaattattca aagttgatta tgagaaattt
    53281 tgcgggattt tacgcagagt gaaacctgat ataccaccca atcaggcaac ccacatcctg
    53341 cggcatacat tcgcaagcca tttcatgatg aatgggggca atataatcgc actgcaacag
    53401 attctgggac atgcgagcat tcagcagacg atggcctatg cgcaccttgc gcctgactat
    53461 ctgcaaaatg ccgtcgctct gaatccacta aaaggcggag tgacgttata aatttccccc
    53521 tctgagtgtc cacatagtgt ccacactcaa gaaattttga aaccgctcca aacccttaca
    53581 gctttcgata agtaactgtt tttacttttg attgcatgta agtatttgat aaaaaaaacc
    53641 ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac
    53701 tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc
    53761 acgtcacgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc
    53821 ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt
    53881 tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg
    53941 gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg
    54001 gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg
    54061 aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct
    54121 gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca
    54181 gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga
    54241 gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat
    54301 tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct
    54361 gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg
    54421 cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat
    54481 tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc
    54541 acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga
    54601 tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg
    54661 tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca
    54721 aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg
    54781 tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct
    54841 ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt
    54901 gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg
    54961 tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg
    55021 ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc
    55081 tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc
    55141 cagatgaccg agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc
    55201 gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg
    55261 gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc
    55321 gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat
    55381 aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc
    55441 gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa
    55501 tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg
    55561 gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg
    55621 aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg
    55681 gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag
    55741 cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg
    55801 ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa
    55861 ctcgagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg
    55921 atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg
    55981 tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca
    56041 gttaacttcc cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg
    56101 ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt
    56161 gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg
    56221 gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa
    56281 tctggcggta caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc
    56341 tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg
    56401 tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc
    56461 atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg
    56521 ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg
    56581 catcgtccgc gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc
    56641 gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac
    56701 acgggaaacc aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata
    56761 caaccacccc accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg
    56821 gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca
    56881 gcggaaaata tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc
    56941 gccaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag
    57001 atagtgctca cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc
    57061 cgcctgctcg tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat
    57121 tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc
    57181 aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt
    57241 ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg
    57301 acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta
    57361 aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg
    57421 gcaccagaat cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca
    57481 tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg
    57541 cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga
    57601 taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac
    57661 cagtctgggc aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa
    57721 tcagcgtttg cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg
    57781 caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa
    57841 tcccggcagt gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca
    57901 caaatgcccc agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc
    57961 cgttggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa
    58021 cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg
    58081 caattttagt taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt
    58141 ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca
    58201 tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg
    58261 cgccaaggaa tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta
    58321 tctgcatttc catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga
    58381 gcatcagtgt tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac
    58441 cacatcaatt gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat
    58501 cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac
    58561 gcctcattgc agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg
    58621 tcccagttca ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc
    58681 aggtagtaag catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa
    58741 atagcttcac ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg
    58801 cctttcagca ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat
    58861 tctgctttga agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc
    58921 tgcagggtgg tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca
    58981 gcgttgttgc gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc
    59041 tcttcaaccg gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt
    59101 acgtaggtct gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt
    59161 tccagggcat cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt
    59221 attgtcgggc ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa
    59281 tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa
    59341 tgcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg
    59401 gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg
    59461 tgaagcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt
    59521 ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa
    59581 aaaatggtca tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc
    59641 gtggaccatc agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct
    59701 ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta
    59761 tggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac
    59821 gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa
    59881 caccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgtagggat
    59941 tgtaactcga gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa
    60001 tctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt
    60061 tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact
    60121 gtatgtcgct ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg
    60181 tttctgtttt attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct
    60241 ggcaaaaccg ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta
    60301 tctgcaattc tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat
    60361 cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct
    60421 gaaagagtgg aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt
    60481 gattattgtc gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt
    60541 gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata
    60601 gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg
    60661 gagaagatat tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc
    60721 agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa
    60781 tttatccagc gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag
    60841 caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa
    60901 catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact
    60961 actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa
    61021 gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct gccagtcaag
    61081 attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga
    61141 agcgtaggag tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata
    61201 aggacgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa
    61261 atcatgtgta tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg
    61321 ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac
    61381 ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt
    61441 cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact
    61501 ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt
    61561 cagtaacgct ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt
    61621 tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca
    61681 gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct
    61741 ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac
    61801 tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg
    61861 gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca
    61921 atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca
    61981 actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca
    62041 gacacagatt atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg
    62101 tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga
    62161 caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg
    62221 gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca
    62281 tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc
    62341 ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt
    62401 ttcaggaaat gcggtgagca tcacatcacc acaatgaagc aaattgtgaa catcatcacg
    62461 ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat
    62521 tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc
    62581 aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag
    62641 cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc
    62701 cagaacggtt atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta
    62761 aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg
    62821 gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat
    62881 agccgctcca atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat
    62941 caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg
    63001 gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc
    63061 agctatcgcc tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg
    63121 gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa
    63181 gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag
    63241 ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc
    63301 tcgccgttaa acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc
    63361 ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa
    63421 ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag
    63481 cgagtgcttc aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca
    63541 cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg
    63601 atgtgcagcg aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat
    63661 gctgaactgg cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat
    63721 gagctggcgc agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc
    63781 cagaacacgc tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg
    63841 cctgttgaag cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc
    63901 aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc
    63961 cctaatcctg acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca
    64021 aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt
    64081 tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt
    64141 caatgcactg ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga
    64201 ccggtgggat tcaggctact ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac
    64261 gagccgatct ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg
    64321 ccatctatct ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca
    64381 aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt
    64441 tctcgcatcc gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc
    64501 agttctggat tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac
    64561 tcggcacacc atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg
    64621 accgtctcgc cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc
    64681 tggatccggc gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga
    64741 gctacacggt tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc
    64801 tgtgcctgga cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg
    64861 ccatgctgta tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg
    64921 atcacgtggt gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg
    64981 atctgttcga ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg
    65041 ccgcgtgggt cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac
    65101 ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac
    65161 tggaagaaca gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg
    65221 atacgccagc aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga
    65281 gccagcgtgg gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga
    65341 atgaccatgc gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa
    65401 acatagcctc gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg
    65461 gggagagggt tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt
    65521 acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa
    65581 gccaccaccg atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg
    65641 cgcctggatg acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat
    65701 attcccctca gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc
    65761 ggcaaattct tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc
    65821 gacagcgacg gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact
    65881 tccgaacttc cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa
    65941 gatcgggtga tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa
    66001 aacgacaccg cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta
    66061 ttcccggatg gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag
    66121 gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc
    66181 ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca
    66241 caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag
    66301 ttgatagcgc tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa
    66361 ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag
    66421 tatcagcgtc ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt
    66481 gcgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag
    66541 attgaatctg aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg
    66601 aaatatatga ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg
    66661 caagaagtgt tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat
    66721 ttta
//
LOCUS       DAZRVY010000007        64591 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZRVY010000007 DAZRVY010000000
VERSION     DAZRVY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.007"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHXOX_000448"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHXOX_000448"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR7004023.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHXOX_000449"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHXOX_000449"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR7004024.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHXOX_000450"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHXOX_000450"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR7004025.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHXOX_000451"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHXOX_000451"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR7004026.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHXOX_000452"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHXOX_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR7004027.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHXOX_000453"
     CDS             complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHXOX_000453"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR7004028.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHXOX_000454"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHXOX_000454"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR7004029.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="ACHXOX_000455"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="ACHXOX_000455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR7004030.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHXOX_000456"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="ACHXOX_000456"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR7004031.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="ACHXOX_000457"
     CDS             complement(12798..14138)
                     /locus_tag="ACHXOX_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR7004032.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHXOX_000458"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="ACHXOX_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR7004033.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="ACHXOX_000459"
     CDS             complement(15927..16376)
                     /locus_tag="ACHXOX_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR7004034.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHXOX_000460"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="ACHXOX_000460"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR7004035.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="ACHXOX_000461"
     CDS             complement(17176..17505)
                     /locus_tag="ACHXOX_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR7004036.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHXOX_000462"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="ACHXOX_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR7004037.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="ACHXOX_000463"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="ACHXOX_000463"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR7004038.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHXOX_000464"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="ACHXOX_000464"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR7004039.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="ACHXOX_000465"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="ACHXOX_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR7004040.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="ACHXOX_000466"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="ACHXOX_000466"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR7004041.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="ACHXOX_000467"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="ACHXOX_000467"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR7004042.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="ACHXOX_000468"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="ACHXOX_000468"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR7004043.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="ACHXOX_000469"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="ACHXOX_000469"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR7004044.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="ACHXOX_000470"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="ACHXOX_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR7004045.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="ACHXOX_000471"
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="ACHXOX_000471"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR7004046.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="ACHXOX_000472"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="ACHXOX_000472"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR7004047.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVQEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="ACHXOX_000473"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="ACHXOX_000473"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR7004048.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="ACHXOX_000474"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="ACHXOX_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR7004049.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="ACHXOX_000475"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="ACHXOX_000475"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR7004050.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="ACHXOX_000476"
     CDS             complement(34677..35072)
                     /locus_tag="ACHXOX_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR7004051.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="ACHXOX_000477"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="ACHXOX_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR7004052.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="ACHXOX_000478"
     CDS             complement(36359..36586)
                     /locus_tag="ACHXOX_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR7004053.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="ACHXOX_000479"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="ACHXOX_000479"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR7004054.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="ACHXOX_000480"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="ACHXOX_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR7004055.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="ACHXOX_000481"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="ACHXOX_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR7004056.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="ACHXOX_000482"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="ACHXOX_000482"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR7004057.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="ACHXOX_000483"
     tRNA            40666..40742
                     /locus_tag="ACHXOX_000483"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="ACHXOX_000484"
     CDS             complement(40817..42070)
                     /gene="amiC"
                     /locus_tag="ACHXOX_000484"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR7004058.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42302..43633
                     /gene="argA"
                     /locus_tag="ACHXOX_000485"
     CDS             42302..43633
                     /gene="argA"
                     /locus_tag="ACHXOX_000485"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR7004059.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43695..45521)
                     /gene="recD"
                     /locus_tag="ACHXOX_000486"
     CDS             complement(43695..45521)
                     /gene="recD"
                     /locus_tag="ACHXOX_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR7004060.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45521..49063)
                     /gene="recB"
                     /locus_tag="ACHXOX_000487"
     CDS             complement(45521..49063)
                     /gene="recB"
                     /locus_tag="ACHXOX_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR7004061.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="ACHXOX_000488"
     CDS             complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="ACHXOX_000488"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR7004062.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52120..55488)
                     /gene="recC"
                     /locus_tag="ACHXOX_000489"
     CDS             complement(52120..55488)
                     /gene="recC"
                     /locus_tag="ACHXOX_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR7004063.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLSPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55501..55824)
                     /locus_tag="ACHXOX_000490"
     CDS             complement(55501..55824)
                     /locus_tag="ACHXOX_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR7004064.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55809..56216)
                     /locus_tag="ACHXOX_000491"
     CDS             complement(55809..56216)
                     /locus_tag="ACHXOX_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR7004065.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56213..56776)
                     /locus_tag="ACHXOX_000492"
     CDS             complement(56213..56776)
                     /locus_tag="ACHXOX_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR7004066.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56767..57237)
                     /locus_tag="ACHXOX_000493"
                     /pseudo
     CDS             complement(56767..57237)
                     /locus_tag="ACHXOX_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="ACHXOX_000494"
     CDS             complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="ACHXOX_000494"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR7004067.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="ACHXOX_000495"
     CDS             complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="ACHXOX_000495"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR7004068.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_000496"
     CDS             complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_000496"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR7004069.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="ACHXOX_000497"
     CDS             complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="ACHXOX_000497"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR7004070.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62391..62537)
                     /gene="ygdT"
                     /locus_tag="ACHXOX_000498"
     CDS             complement(62391..62537)
                     /gene="ygdT"
                     /locus_tag="ACHXOX_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR7004071.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62723..63412
                     /gene="mutH"
                     /locus_tag="ACHXOX_000499"
     CDS             62723..63412
                     /gene="mutH"
                     /locus_tag="ACHXOX_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR7004072.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63481..64194
                     /locus_tag="ACHXOX_000500"
     CDS             63481..64194
                     /locus_tag="ACHXOX_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR7004073.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64332..64550
                     /gene="ygdR"
                     /locus_tag="ACHXOX_000501"
     CDS             64332..64550
                     /gene="ygdR"
                     /locus_tag="ACHXOX_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR7004074.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15654 a        17369 c        15824 g        15744 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga aacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc aaatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acgcgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagcg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccgggt tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgtcacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccagaaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttctaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagccagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc aggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgca ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattattga acaccctaac gggtgttttt ttgtttctac tctcccataa aaaaagcgcc
    40801 attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca aaatacgctt
    40861 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    40921 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    40981 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    41041 cgagcttatt cagcactgct ttaccaaact taaggctgtc agcaatggtc agcgactgca
    41101 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    41161 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    41221 ttgagagcgc aaacaccgag gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga
    41281 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa
    41341 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    41401 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    41461 ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc
    41521 gccctgcttt acctggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc
    41581 ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg
    41641 gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct
    41701 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    41761 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    41821 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg ttactcaacg
    41881 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    41941 caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc tgacttacgc
    42001 tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    42061 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    42121 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    42181 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    42241 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc
    42301 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    42361 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    42421 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    42481 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    42541 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    42601 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    42661 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    42721 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    42781 gatccgtcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    42841 cactggcgag agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact
    42901 gaaagctgag aagatgattg gtttttgctc ttcacagggc gtcactaatg acgacggtga
    42961 tattgtctca gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    43021 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    43081 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    43141 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    43201 cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    43261 tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    43321 tcagctcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    43381 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt
    43441 tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    43501 gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg atattgattt
    43561 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    43621 ggatttaggg taagcgatga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca
    43681 tccggcaatt acgtttatcc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    43741 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    43801 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctatcggcgt gcgttggctc
    43861 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    43921 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    43981 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    44041 aacaacccaa gcgcactgtc attacgggca atcatcaccg gtcgaccttc gtaccaacga
    44101 gagtgcgaat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    44161 agtccagcca cgccaaatgg cccttcccgc agggcgcaca aaagttggta ctcattgaac
    44221 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccataa
    44281 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagccgtttt
    44341 tcaatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg
    44401 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    44461 aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt
    44521 cccgtcaggc ggcttagctg cccggcacgc tcggcggtaa agccggcatt ggcataagcg
    44581 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    44641 aagatcactc gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    44701 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    44761 cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc
    44821 ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat
    44881 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    44941 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    45001 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgctgc cgccactttt
    45061 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    45121 tcatcaacct caatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    45181 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    45241 cccctgctga ccgcttgaga agccagcaag cattcttccc aattttgtag ctcaccgatt
    45301 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    45361 ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg
    45421 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    45481 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    45541 taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa
    45601 tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    45661 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg
    45721 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    45781 gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac
    45841 gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga
    45901 actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct
    45961 ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc
    46021 ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg
    46081 tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    46141 cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca
    46201 agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    46261 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    46321 cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    46381 gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt
    46441 tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    46501 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    46561 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    46621 gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    46681 gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc aaaactgcct
    46741 caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg
    46801 gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca atctgcacca
    46861 gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga
    46921 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    46981 gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    47041 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    47101 gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc cacgcatgtt
    47161 cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg
    47221 cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt
    47281 cctgcgcttc cagagtttca aaaacactat cgcggttcga aaggtaaacg gaagggattt
    47341 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacgc
    47401 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    47461 gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    47521 gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    47581 tttcaccttt aaagacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    47641 acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg ttcacactgt
    47701 tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac
    47761 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    47821 ggtcgccaat cagcaacaat gcggtttctg gctgatggtg ccagatacgg cgaaaaattc
    47881 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    47941 ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta
    48001 acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga
    48061 tctcagccaa tgcgcgggtg atcaccagat cacggatcga caatggttct gcaagcagtt
    48121 gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt
    48181 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    48241 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    48301 gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    48361 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat
    48421 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    48481 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    48541 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    48601 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    48661 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    48721 acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggtgt tcgtacaatg
    48781 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    48841 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    48901 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaataaagcg
    48961 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta
    49021 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    49081 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca
    49141 tattcggctt tcccgttttg gctgccggaa atctgcgaca ggatagccat gccttgcggc
    49201 tcgactaccg cctgatggaa gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg
    49261 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    49321 ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc
    49381 tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa
    49441 aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag
    49501 cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt
    49561 tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta
    49621 taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag
    49681 tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct
    49741 ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc gcgtgccagc
    49801 gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat
    49861 tttaaggcgt cgcgataagc cagcacctct ttcaacgtaa tggagggcaa aattttacgc
    49921 cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc
    49981 tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc
    50041 tcaagctgat cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc
    50101 tgcggcagac gctgggggta accattagca ttaaccataa ggccgttgtt agcgttggtg
    50161 gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg
    50221 agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta
    50281 cgcaaaatca ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatac
    50341 accacgcgca gattcgactc atcaacaatc agctctggat ggtcatattt cttctctgac
    50401 ttaatcagcg agaaatcgtc aggaatatag gggttaagct ccggtaagga gagcgcaata
    50461 ttggcagctt tttgctgcca gtcagcgaaa gtttgttcgc tgattttatc gacctgatac
    50521 ggcgcatcga caaaataagc cgttttattg tgcggctctt tcgggctgat ataccagata
    50581 cgcgcattct gcggcgtcat catcgccaga cgttctttta ctgctttagc atcgtaccga
    50641 tcggcaatat tgactgcatc cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc
    50701 agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc gatatccagc
    50761 acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa cagattgaga
    50821 tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc ggttaaagac
    50881 gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg
    50941 ccctcaacta atccctgctt ttgcagccag tcggaaagtg tacctgggct gcgattacca
    51001 atcagatagg taatcaattc atccgtttta ctacggaact tcgctgagtt gttatcgatg
    51061 cgaaactcaa cgcgcagaac tttacgcggc attgccggga cgtaatgaat gataatgccc
    51121 ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc
    51181 acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa
    51241 ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc
    51301 tgcaccggat taccaggttt atcgcttaaa gtttcgaggt taccaccaga aaactttgaa
    51361 ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc
    51421 gtacgcgcca tggttaattc agcgttcacc gcattacgct cacgttcggc atatttcttg
    51481 tcgagcaaag gttcagcaat agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg
    51541 ttctcaactt ccagatagaa agccgtgcga tacggcgcag tgctggcatt gtgactaccg
    51601 ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc
    51661 agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac
    51721 ccaacgggca ccaccagcgc cgagagcgat ttaactgcct gcggatcaga aaccagcaag
    51781 accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta
    51841 cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg
    51901 gcaactaaca acaataatgc tttgaaccag gtgctgcggg gcattctcgg acctcataat
    51961 caacttaatt ttctgtccag attcaacacg ttaacgcaaa tcatctgcca gaatttaatc
    52021 ttgtgctgca cgagtcagcc tatgtttata taaccatcag tccgtgactg gtgcgcatca
    52081 taaagtaagc ggatagattg cgcaattttt atacagcact catgactgat taaagcgaaa
    52141 cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct ctggtgttaa
    52201 ttgccgccag agcctttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc
    52261 ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat
    52321 ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac
    52381 cagcaatggc gcggacatcc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa
    52441 agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg
    52501 actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc
    52561 ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg
    52621 caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg
    52681 ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gagtttccca
    52741 gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc gacggaacaa
    52801 gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg
    52861 gctaagccct tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa
    52921 gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag
    52981 cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg
    53041 tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc
    53101 tggctgatag ttttgtggat caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt
    53161 tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg
    53221 cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg
    53281 aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag
    53341 gaacagatag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat
    53401 caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca ttcccagcag
    53461 gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc
    53521 ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt catcacgcaa
    53581 tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg
    53641 ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc
    53701 gctgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct gtgccagccc
    53761 gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat
    53821 taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat
    53881 cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg
    53941 gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac
    54001 ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg
    54061 cacatccagt aatgccagca cgtcttccga cacaaagcgg ctgtctggca acgataacag
    54121 gctgataaac gcttccagta ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta
    54181 aggcaggtaa cgatccgcag gtgcgctacc aaacacagcc cgaataaacg gactgtagct
    54241 gtcgatatca gccaccatca cgatgatgtc gcgcggacta agtgtcgggt cttcttccag
    54301 catcgccagc aggcgatcgt gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg
    54361 gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc
    54421 ttcgatgttc acaccagcaa cggcacggtt ttccagttcc agaatgtcag actgaatgtt
    54481 atgcagcagg ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag
    54541 gtcagaaagg agataaatgt agtcgcgccc aagcttaccc catgaagcca gcagcgggtt
    54601 gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa
    54661 tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgggtcagca gtttcgccag
    54721 ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg
    54781 gatttcaata tgtttaccca gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga
    54841 aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag
    54901 cgtttcgata aagcgctgat agagattggc gcggtgccag cgcggttgcc cgagttgatg
    54961 ggtatattcc accagcgcct tccacaacgg ggcttgccag gcctgtgctt ctcccagccc
    55021 ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga ccagatactg
    55081 gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc
    55141 agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt
    55201 catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tttccggtaa
    55261 cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc
    55321 aatactaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac
    55381 cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat
    55441 caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga
    55501 ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac
    55561 tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca
    55621 gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg
    55681 ttggtactgg tttctgctgg cgagactgtt cattaatgtt cgctggatac ccgataacgc
    55741 agtgacaatc atcaccatca gcaccatcgc aaccattacc tccggcaggc taaagccttg
    55801 ttgattcttc agggaagctg acataacgcc ctctctttca gcggacaaaa atcgctccag
    55861 ccgcgtggcg aaaaaacaat gtttccatca atgacttcgc ctgttcgcca caacgaaacg
    55921 ccttcataac cggcaaccaa taaggcttca ttatctgcca gtaaacgcaa acaaacccgg
    55981 gcatcggttc cagcgtactg cgagcactga actgcgggct gcgtctgcca ggagtgcatt
    56041 ttcccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttctatg
    56101 ctcacgcgag aggcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc
    56161 aaaaccagca gcatcaggac cagagccagt gacgaaacac ctttttcgcg gttcacaggt
    56221 tgaatcctgt cacgctatag ctggcatcca ccacggtttg cggttcagac ttactggcag
    56281 cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa
    56341 aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg
    56401 atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcag
    56461 actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga
    56521 tacagtcacc ctgtccgaca atttccagcc cttcgccggt acagctgcca tgacaataac
    56581 ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg
    56641 ccagcttacg ggtgctcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc
    56701 ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt
    56761 gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg
    56821 cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc
    56881 ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt agacttcggg
    56941 ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga
    57001 actcacaagg caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg
    57061 ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca
    57121 tagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcgc ttagcatgac
    57181 caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc
    57241 ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct
    57301 tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat
    57361 ttaacggagg gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta
    57421 attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca atctcaaagt
    57481 cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg
    57541 gacgcggttc gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg
    57601 tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat
    57661 gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc
    57721 gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc
    57781 acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc
    57841 gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac
    57901 catctggcgt tggccaggcg cgccactgtt taccatacac tggtccgagg tcgccgtttt
    57961 catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt
    58021 cgccctgcag aaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttagttg
    58081 tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg
    58141 ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt
    58201 ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc
    58261 agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga
    58321 tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga
    58381 tgcgaaacgc gccgtagcca atcaggaaca agccagagac agctcccatt gggcgcggtt
    58441 tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc agctcgtaaa
    58501 gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt
    58561 tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga
    58621 agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac
    58681 caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag gaacgtttag
    58741 tacggcgggc aaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca
    58801 tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt
    58861 agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta
    58921 agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt cgtgttgcca
    58981 gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg
    59041 gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct
    59101 gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag
    59161 gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata
    59221 ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc
    59281 cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca
    59341 gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc aggtatttta
    59401 cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg
    59461 ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt
    59521 ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa
    59581 tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg
    59641 tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg
    59701 gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga
    59761 cttcacgtcc ggcacgttca atcaggcggc gtgcttcatc aacttcatcg aggcttgtga
    59821 ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca
    59881 cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc
    59941 atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg
    60001 tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct
    60061 gttcttcttc cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac
    60121 cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga
    60181 gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg
    60241 ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg
    60301 gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca
    60361 gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga
    60421 tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg
    60481 gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc
    60541 aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac
    60601 gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa
    60661 atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa
    60721 acaattcgcg acgcagccgg gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg
    60781 ccgtttcttt ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg
    60841 cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct
    60901 gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac
    60961 caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca
    61021 actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt
    61081 cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc
    61141 gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga
    61201 tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc
    61261 caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt
    61321 tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg
    61381 aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat
    61441 tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg
    61501 gcgtgactta acctctttta cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt
    61561 cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt
    61621 taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact
    61681 ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa
    61741 accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg
    61801 gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta
    61861 atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga
    61921 ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc
    61981 cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg
    62041 gactacctca aaataaagct ttatatacga atgattgttt catactccaa gaagacggta
    62101 aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca
    62161 gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag
    62221 ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat
    62281 agacatttta aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa
    62341 gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg
    62401 ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt
    62461 tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag
    62521 ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac
    62581 ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa
    62641 ttgaacaaca catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt
    62701 tttttaacca aggtatcatg acatgtccca acctcgccca ctcctctctc ctcccgaaac
    62761 tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc
    62821 ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt
    62881 gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc
    62941 tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac
    63001 attcgtttgt gttgccccgt taacgggtaa tagcggggtg acctgggaaa ccagccatgt
    63061 gcgccacaag ctcaaacgcg tactgtggat accggttgaa ggtgagcgca gcatcccgct
    63121 ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca
    63181 gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat
    63241 caccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct
    63301 taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt
    63361 gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct
    63421 ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag
    63481 atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg
    63541 ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctc
    63601 cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt
    63661 ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata
    63721 ttcagtcagg aaatttccgc ccgtgatttg attctgcttc tgggtggctt gttccttatc
    63781 tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca
    63841 cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc
    63901 ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggccgcc
    63961 gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa
    64021 cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtcgg ctttaccctg
    64081 attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc
    64141 tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct ctgatactcc
    64201 gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc
    64261 tgctttcaag atatataacg tcggtttata aacagactat tatcataggt gggccaaatg
    64321 aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg
    64381 tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg
    64441 atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta
    64501 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
    64561 cagcatccgg ctgtaatgcc gatcagttaa g
//
LOCUS       DAZRVY010000008        60957 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZRVY010000008 DAZRVY010000000
VERSION     DAZRVY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.008"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHXOX_000502"
     CDS             <1..468
                     /locus_tag="ACHXOX_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7004075.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHXOX_000503"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHXOX_000503"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR7004076.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHXOX_000504"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHXOX_000504"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR7004077.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHXOX_000505"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHXOX_000505"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR7004078.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHXOX_000506"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHXOX_000506"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR7004079.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHXOX_000507"
     CDS             complement(4084..5313)
                     /locus_tag="ACHXOX_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR7004080.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHXOX_000508"
     CDS             complement(5377..5817)
                     /locus_tag="ACHXOX_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR7004081.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHXOX_000509"
     CDS             complement(5922..6692)
                     /locus_tag="ACHXOX_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7004082.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHXOX_000510"
     CDS             complement(6689..7615)
                     /locus_tag="ACHXOX_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7004083.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHXOX_000511"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHXOX_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR7004084.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHXOX_000512"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHXOX_000512"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR7004085.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHXOX_000513"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHXOX_000513"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR7004086.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHXOX_000514"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHXOX_000514"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR7004087.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHXOX_000515"
     CDS             13322..13669
                     /locus_tag="ACHXOX_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR7004088.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHXOX_000516"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHXOX_000516"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR7004089.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHXOX_000517"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHXOX_000517"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR7004090.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHXOX_000518"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHXOX_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR7004091.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHXOX_000519"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHXOX_000519"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR7004092.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHXOX_000520"
     CDS             18978..20735
                     /locus_tag="ACHXOX_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR7004093.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHXOX_000521"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHXOX_000521"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR7004094.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHXOX_000522"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHXOX_000522"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR7004095.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHXOX_000523"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHXOX_000523"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR7004096.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHXOX_000524"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHXOX_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR7004097.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHXOX_000525"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHXOX_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR7004098.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHXOX_000526"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHXOX_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR7004099.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHXOX_000527"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHXOX_000527"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR7004100.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHXOX_000528"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHXOX_000528"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR7004101.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHXOX_000529"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHXOX_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR7004102.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHXOX_000530"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHXOX_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR7004103.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHXOX_000531"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHXOX_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR7004104.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHXOX_000532"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHXOX_000532"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR7004105.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHXOX_000533"
     CDS             complement(36854..36898)
                     /locus_tag="ACHXOX_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004106.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHXOX_000534"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHXOX_000534"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR7004107.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHXOX_000535"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHXOX_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR7004108.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHXOX_000536"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHXOX_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR7004109.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHXOX_000537"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHXOX_000537"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR7004110.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHXOX_000538"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHXOX_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR7004111.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHXOX_000539"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHXOX_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR7004112.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHXOX_000540"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHXOX_000540"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR7004113.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHXOX_000541"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHXOX_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR7004114.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHXOX_000542"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHXOX_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR7004115.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHXOX_000543"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHXOX_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR7004116.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHXOX_000544"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHXOX_000544"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR7004117.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHXOX_000545"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHXOX_000545"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR7004118.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHXOX_000546"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHXOX_000546"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR7004119.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHXOX_000547"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHXOX_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR7004120.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHXOX_000548"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHXOX_000548"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR7004121.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHXOX_000549"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHXOX_000549"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR7004122.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHXOX_000550"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHXOX_000550"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR7004123.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHXOX_000551"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHXOX_000551"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR7004124.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHXOX_000552"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHXOX_000552"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR7004125.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHXOX_000553"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHXOX_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR7004126.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHXOX_000554"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHXOX_000554"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR7004127.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHXOX_000555"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHXOX_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR7004128.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16852 c        15000 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttacc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcct cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZRVY010000009        59896 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZRVY010000009 DAZRVY010000000
VERSION     DAZRVY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.009"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHXOX_000556"
     CDS             complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHXOX_000556"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR7004129.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
     gene            1594..2034
                     /locus_tag="ACHXOX_000557"
     CDS             1594..2034
                     /locus_tag="ACHXOX_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR7004130.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHXOX_000558"
     CDS             complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHXOX_000558"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR7004131.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHXOX_000559"
     CDS             complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHXOX_000559"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR7004132.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHXOX_000560"
     CDS             complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHXOX_000560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR7004133.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHXOX_000561"
     CDS             complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHXOX_000561"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR7004134.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHXOX_000562"
                     /pseudo
     CDS             complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHXOX_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHXOX_000563"
     CDS             complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHXOX_000563"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR7004135.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHXOX_000564"
     CDS             complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHXOX_000564"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR7004136.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHXOX_000565"
     CDS             complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHXOX_000565"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR7004137.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHXOX_000566"
     CDS             complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHXOX_000566"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR7004138.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHXOX_000567"
     CDS             complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHXOX_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR7004139.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            12268..12651
                     /gene="panM"
                     /locus_tag="ACHXOX_000568"
     CDS             12268..12651
                     /gene="panM"
                     /locus_tag="ACHXOX_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR7004140.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHXOX_000569"
     CDS             complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHXOX_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR7004141.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHXOX_000570"
     CDS             complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHXOX_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR7004142.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHXOX_000571"
     CDS             complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHXOX_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR7004143.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHXOX_000572"
     CDS             complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHXOX_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR7004144.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHXOX_000573"
     CDS             complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHXOX_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR7004145.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHXOX_000574"
     CDS             18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHXOX_000574"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR7004146.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19277..19546
                     /locus_tag="ACHXOX_000575"
     CDS             19277..19546
                     /locus_tag="ACHXOX_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR7004147.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19549..19908)
                     /locus_tag="ACHXOX_000576"
     CDS             complement(19549..19908)
                     /locus_tag="ACHXOX_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR7004148.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            20049..20675
                     /locus_tag="ACHXOX_000577"
     CDS             20049..20675
                     /locus_tag="ACHXOX_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR7004149.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20749..22947
                     /gene="zntA"
                     /locus_tag="ACHXOX_000578"
     CDS             20749..22947
                     /gene="zntA"
                     /locus_tag="ACHXOX_000578"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR7004150.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHXOX_000579"
     CDS             complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHXOX_000579"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR7004151.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            23515..24180
                     /locus_tag="ACHXOX_000580"
     CDS             23515..24180
                     /locus_tag="ACHXOX_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR7004152.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHXOX_000581"
     CDS             24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHXOX_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR7004153.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24814..26031)
                     /locus_tag="ACHXOX_000582"
     CDS             complement(24814..26031)
                     /locus_tag="ACHXOX_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7004154.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            26163..27212
                     /locus_tag="ACHXOX_000583"
     CDS             26163..27212
                     /locus_tag="ACHXOX_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR7004155.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            27267..27854
                     /gene="acpT"
                     /locus_tag="ACHXOX_000584"
     CDS             27267..27854
                     /gene="acpT"
                     /locus_tag="ACHXOX_000584"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR7004156.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            27965..29539
                     /gene="nikA"
                     /locus_tag="ACHXOX_000585"
     CDS             27965..29539
                     /gene="nikA"
                     /locus_tag="ACHXOX_000585"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7004157.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            29539..30483
                     /gene="nikB"
                     /locus_tag="ACHXOX_000586"
     CDS             29539..30483
                     /gene="nikB"
                     /locus_tag="ACHXOX_000586"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR7004158.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            30480..31313
                     /gene="nikC"
                     /locus_tag="ACHXOX_000587"
     CDS             30480..31313
                     /gene="nikC"
                     /locus_tag="ACHXOX_000587"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR7004159.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            31313..32077
                     /gene="nikD"
                     /locus_tag="ACHXOX_000588"
     CDS             31313..32077
                     /gene="nikD"
                     /locus_tag="ACHXOX_000588"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR7004160.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            32074..32880
                     /gene="nikE"
                     /locus_tag="ACHXOX_000589"
     CDS             32074..32880
                     /gene="nikE"
                     /locus_tag="ACHXOX_000589"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR7004161.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            32886..33287
                     /gene="nikR"
                     /locus_tag="ACHXOX_000590"
     CDS             32886..33287
                     /gene="nikR"
                     /locus_tag="ACHXOX_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR7004162.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(33407..33766)
                     /locus_tag="ACHXOX_000591"
     CDS             complement(33407..33766)
                     /locus_tag="ACHXOX_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR7004163.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(33763..34036)
                     /locus_tag="ACHXOX_000592"
                     /pseudo
     CDS             complement(33763..34036)
                     /locus_tag="ACHXOX_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34109..35233)
                     /locus_tag="ACHXOX_000593"
     CDS             complement(34109..35233)
                     /locus_tag="ACHXOX_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7004164.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="ACHXOX_000594"
     CDS             complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="ACHXOX_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR7004165.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(37965..39032)
                     /locus_tag="ACHXOX_000595"
     CDS             complement(37965..39032)
                     /locus_tag="ACHXOX_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR7004166.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(39095..39208)
                     /gene="yhiY"
                     /locus_tag="ACHXOX_000596"
     CDS             complement(39095..39208)
                     /gene="yhiY"
                     /locus_tag="ACHXOX_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR7004167.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(39398..41020)
                     /locus_tag="ACHXOX_000597"
     CDS             complement(39398..41020)
                     /locus_tag="ACHXOX_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR7004168.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFSDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(41282..42889)
                     /locus_tag="ACHXOX_000598"
     CDS             complement(41282..42889)
                     /locus_tag="ACHXOX_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR7004169.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            43274..44326
                     /locus_tag="ACHXOX_000599"
     CDS             43274..44326
                     /locus_tag="ACHXOX_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR7004170.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(44642..45844)
                     /locus_tag="ACHXOX_000600"
     CDS             complement(44642..45844)
                     /locus_tag="ACHXOX_000600"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR7004171.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            46076..47575
                     /gene="pitA"
                     /locus_tag="ACHXOX_000601"
     CDS             46076..47575
                     /gene="pitA"
                     /locus_tag="ACHXOX_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR7004172.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="ACHXOX_000602"
     CDS             complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="ACHXOX_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR7004173.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            48546..48980
                     /gene="uspA"
                     /locus_tag="ACHXOX_000603"
     CDS             48546..48980
                     /gene="uspA"
                     /locus_tag="ACHXOX_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR7004174.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(49079..49132)
                     /locus_tag="ACHXOX_000604"
     CDS             complement(49079..49132)
                     /locus_tag="ACHXOX_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004175.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            49298..50767
                     /gene="dtpB"
                     /locus_tag="ACHXOX_000605"
     CDS             49298..50767
                     /gene="dtpB"
                     /locus_tag="ACHXOX_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR7004176.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(50816..51568)
                     /gene="rsmJ"
                     /locus_tag="ACHXOX_000606"
     CDS             complement(50816..51568)
                     /gene="rsmJ"
                     /locus_tag="ACHXOX_000606"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR7004177.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(51576..53618)
                     /gene="prlC"
                     /locus_tag="ACHXOX_000607"
     CDS             complement(51576..53618)
                     /gene="prlC"
                     /locus_tag="ACHXOX_000607"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR7004178.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            53821..54663
                     /gene="rlmJ"
                     /locus_tag="ACHXOX_000608"
     CDS             53821..54663
                     /gene="rlmJ"
                     /locus_tag="ACHXOX_000608"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR7004179.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            54735..56087
                     /gene="gorA"
                     /locus_tag="ACHXOX_000609"
     CDS             54735..56087
                     /gene="gorA"
                     /locus_tag="ACHXOX_000609"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR7004180.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(56141..56224)
                     /gene="dinQ"
                     /locus_tag="ACHXOX_000610"
     CDS             complement(56141..56224)
                     /gene="dinQ"
                     /locus_tag="ACHXOX_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR7004181.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            56252..56425
                     /locus_tag="ACHXOX_000611"
     CDS             56252..56425
                     /locus_tag="ACHXOX_000611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004182.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            56964..57317
                     /gene="arsR"
                     /locus_tag="ACHXOX_000612"
     CDS             56964..57317
                     /gene="arsR"
                     /locus_tag="ACHXOX_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR7004183.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            57371..58660
                     /gene="arsB"
                     /locus_tag="ACHXOX_000613"
     CDS             57371..58660
                     /gene="arsB"
                     /locus_tag="ACHXOX_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR7004184.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            58673..59098
                     /gene="arsC"
                     /locus_tag="ACHXOX_000614"
     CDS             58673..59098
                     /gene="arsC"
                     /locus_tag="ACHXOX_000614"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR7004185.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(59232..59528)
                     /locus_tag="ACHXOX_000615"
     CDS             complement(59232..59528)
                     /locus_tag="ACHXOX_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR7004186.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            59635..>59896
                     /locus_tag="ACHXOX_000616"
     CDS             59635..>59896
                     /locus_tag="ACHXOX_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7004187.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT        14010 a        16148 c        15123 g        14615 t
ORIGIN      
        1 gcacggtggt aataatccga ctcccgccag gactaccggt aaccagccaa gttttaccgt
       61 ctttcaccac aatggttggt gacatcgatg acagcggacg tttattcggc ccgacggcgt
      121 tggcatcacc gcccaccagc ccgtaaatgt tcggtacgcc cggtttggcg gagaaatcat
      181 ccatctgatt attaagcaga ataccgctct cgcccgcgac aatgcccata ccgaaggtgg
      241 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccactaca gagtaatggg
      301 tagtttgatt actctcataa ggcgcaagct tgccagggcg aatctcactg gacggcttcg
      361 ctttgttgat atcaatttga tcggcaatgg atttggcata ggctttattg gtcagtgcct
      421 gccacgggac tttgacaaaa tccgggtcgc caagatattc cgagcggtcg gcgtaggcgt
      481 atttctccgc ttctgccatg atttgcatcg catcggcgct gccaaagccg tatttcttca
      541 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
      601 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggagtg cgttcgaccg
      661 ctttataggc cgctaaatct tctttagtga tcaagccacc gtttttctgc atctcctggg
      721 cgatctgttc cgcaatcgtg cctttataga attcgtccgg gccgttttca gcaatcatct
      781 ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc ctttttcagc ggctcgccct
      841 ctttccagaa gatagcttta ctgttttcgt gattcggcaa cacttcgcta ccgtaggttt
      901 tgagatcgtc agccagcgcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgcgg
      961 gctgcacgac tttgttcagc ggcatggtgc cgtatttatc cagcgccagc gagaaacctg
     1021 ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
     1081 tgccctgatc atcgaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
     1141 cgatagccgt ggtattgcca tttttcgagc ggattaacat aaaaccacca ccgcccagat
     1201 tccctgcctg cggatgcgtt accgccagcg cgtagcccac cgccacggcg gcatcaacgg
     1261 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtggcg tccacagacg
     1321 ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc ttcctccaca ccatacgaga
     1381 cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact tcctgagagc agagcagcaa
     1441 tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga ttaaggggca
     1501 accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg gaagcgagta
     1561 aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat tcttacggca
     1621 ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc taaccagccg
     1681 gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca gcaaatccag
     1741 caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca gcagcattta
     1801 gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg ggagcgaaat
     1861 cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag caaccgtaat
     1921 ccggatagtt cgttgaatca gcagcatatg ctgccggaaa ggagaaacgg cgacatgctg
     1981 aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc gtaaagttcg
     2041 gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc agcaactctg
     2101 ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg tctttcaact
     2161 gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg cagcccagcc
     2221 gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca cgcggcagtt
     2281 ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac agcagcggcg
     2341 gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc gttaatggtc
     2401 cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg cgacagcgtt
     2461 ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg taccaactgc
     2521 ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc cagccgttgc
     2581 tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc gataacttcg
     2641 cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc gccagggtgt
     2701 tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa ggccagttac
     2761 tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg ccaaatgcag
     2821 ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca ccagcttctg
     2881 ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg atctccagcg tgtccatcac
     2941 cagcggcaca ccaccttctg cctgcgagct tagcgctata tgttccgggc gaatgccgag
     3001 agtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
     3061 gtccagttcg aaatgcgtgc cttcattatt cacgcggcct gtcagcaggt tcatcgccgg
     3121 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt caactggtgt
     3181 gccaatctgt tcggcaacgc caccgttcat caccattact cgctgggcga gcgtcatcgc
     3241 ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcaactgttg
     3301 cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg ttagagagcg gctcatcaaa
     3361 caagaacacc gccggatcgc gcacaatcgc gcggcccatc gccacacgct ggcgctgacc
     3421 gccggatagc tcacgcggac gacgcttaag cagaccgtcc agttccagaa tgcgcgccgc
     3481 ttctttaacg cgctcggcaa tttgctgctt gcccatgccg cgaattttca gcccccacgc
     3541 catgttctcc tctacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
     3601 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
     3661 tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc gatttcccgc agccagacgg
     3721 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgacctg
     3781 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
     3841 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc accaccggag
     3901 ggataagcgt taacaacatc gccgccatta ccgagttcca ttccgtggtg ccttcgcctg
     3961 tagcgatcat ccctttgatc cctgccacgg tggtgccgag atcaacgtcg gtaataatca
     4021 acaacggcca caaatactga ttccagccgt agataaaggt gatcacaaac agcgccgcca
     4081 gattagtttt ggacaacgga aaaacgatgt cgcaaaagaa acgcattggt gacgcgccgt
     4141 cgatccgcgc ggcttccacc aattcatccg gtagggtcat aaagaactgg cggaacagaa
     4201 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcattt
     4261 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
     4321 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
     4381 atgccgagag catcgagacg gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
     4441 tgttaagcaa catccgccag aacggcgcgc tattcgtgcc taccccgtta acccagatgt
     4501 tgtggatatt ttccagcaga tgtgtgccgg ggatgagcgt catcggcgcg gcatagacgg
     4561 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
     4621 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
     4681 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
     4741 catcaacacc accgactgcg cggcagacga agccagatcc agtccggtaa agccttcgcg
     4801 gtagatctta taaatcagcg tagtggtggc ctgaaccggc ccgccggacg tggcggcgtc
     4861 gatcaccggg aaggtgtcga agaaggcgta caccaggttc accaccagca ggaagaaact
     4921 caccggagcg ataagcggca gagcaatctt aaagaagcgg cgaatcggcc ctgcgccgtc
     4981 aatagctgcg gcttcaatca acgaacgggg aatggagtgc agcgcggcat agaagaacag
     5041 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca ttgcctgacc
     5101 gctgttttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
     5161 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
     5221 cggcagcagc atcaaggttt gatagaaacg gctgccgcgc acgatgtact ccaccagcgc
     5281 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
     5341 cgtccagaag gagtctagat agtagctgtc atggaacagc gtgacgaagt tatccagacc
     5401 gacaaactgg ctggagaaac caaacggatc gacgctttgc cgcgagtacc acaacgcttc
     5461 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
     5521 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
     5581 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
     5641 gcatccgaca ttacactgat taagacttcg tcgatttctc aaagcggcgc agcaactgat
     5701 ttccacgctc aacggcggta tccagtgcct gctgtggtgt cttcttaccg gtccacacgc
     5761 tctccagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgcagccctt
     5821 tggtgaacgg caacggcggc ttattcagca tctgacgcgt cgcggtatcc gcccctgggt
     5881 ttttctcata aaagccctgc tcacgggtca ggtcatacgc tgctttggtg attggcagat
     5941 aaccggtttt ctgatgccac tcggcagcgt tttctggctt cgcgaggaag tcgaggaact
     6001 tcgccacgcc ggtatacgtt tctttatttt tgccctgcat cacccacagg ctggccccgc
     6061 cgataatggc gttttgcggt gcatctttcg catcggcgtc gtaaggcatc atgcctacgc
     6121 catagttaaa tttggcgtac tcacgaatat tagcgagaga accggaagag gcggtggtca
     6181 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
     6241 agtcgccctt cttgttcatc tcttcgagca tggcaatgtg tttcacctgc tccggcttgt
     6301 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
     6361 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
     6421 acttcatacc ggaggctttc agttttgcgg aagagtccgc cagatcctgc caggttttcg
     6481 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
     6541 gagtcgagct gttgaatggc tgggagagta agtggcccgt tttgctgtcg gagtagtaac
     6601 cggaaaccgt cggcacaaac tgcgactcat cgaattgaat ccctgcctct ttaaacacgt
     6661 catataccgg tttaatggct ttcgatgcca tcatggtggc ggtgccaact tcataaacct
     6721 gcaagtaagg tgtcagaaag atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag
     6781 atggggcacg gataagcgga aaaatagaag gtctgaatca aactctacag attactcatc
     6841 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
     6901 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
     6961 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
     7021 tacgcacttc tcaccgcttc attcgccagt agtgcatcac cagtatcgga gagcaccaca
     7081 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
     7141 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
     7201 tggatgataa tcggcgcaag accaagcgat ggctcatcaa gtagcagcaa acgcgggttg
     7261 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
     7321 cgctgaacac ggcgctcatg cagacgtggg aacagttcat acacccactt tatgcgctcc
     7381 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
     7441 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
     7501 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
     7561 tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc aatgagggtg
     7621 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
     7681 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
     7741 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
     7801 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
     7861 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
     7921 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
     7981 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
     8041 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
     8101 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
     8161 tttcagttgt tgatgctgcg ccaccagcag gttttcaatt accgtcattt cacggaacag
     8221 acgcacatgc tggaaggtgc gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
     8281 ttccaggtgc tgattgcgca gtaaaatggt gccgccggtg ggtttgtaga atccggtcag
     8341 acagttaaaa accgtagttt ttccggcacc gttagggccg attaacgaga cgatctcctg
     8401 cgggtacagt tcaagattga cgttgttcac cgccagcagg ccgccgaagc gcatcatcag
     8461 gccgttcaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
     8521 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
     8581 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
     8641 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
     8701 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
     8761 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact gatggtaaag
     8821 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcatcttca
     8881 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
     8941 cttagcacca ccagcagcaa cgccaccaga tacaggaaga tgacgcgatc ggagggatcg
     9001 tatttcaggc caaagaaatt actgaacgtg tcccaaccgc cttcacgggc ggtacggctg
     9061 aactcaaggc cgaagaaagt cggtttcggg atctgactga taccgttcgg gccgccggta
     9121 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
     9181 gccagataat caccgcgcag acgcaacacc ggaaaaccaa gcaggaagcc cgccgccgct
     9241 gccatcagtc cggcaatcgg caaacaggtc cagaacccca gaccgtaata gtgattgagc
     9301 agcgcaaaag tgtaagcgcc gatggcgtaa aaaccgccgt accccagcac cagcagacca
     9361 gaaagaccaa caaccacatt cagcccgaga ccgaggataa tgtagatcat ggtcagagtg
     9421 gcgatatcca ccgtcccacg tgacaccata aacggccacg acaccgcaag caccagcagc
     9481 gccacgagga acagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
     9541 ggcccggaaa cgctttttag ccctttctgg aaagcaggtc gcaaaagctg gaagaaaaag
     9601 accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct gtcgaccacc
     9661 agtttggtgc catccagctc cagttgcacg cccataaaga cgccagcgag gacaaagaac
     9721 atcgcggcag agagcagtgc cattgcaata tgcatcggtt tcatactttc tctacctccg
     9781 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
     9841 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagga
     9901 tcagcccgcc aatcatcgcc cctggaatgc tgccaatccc accgagcacc gcagcggtaa
     9961 aggctttcat cccggccata aagccgatat aggggttaat gacgccgtag aactgaccga
    10021 gcagtacgcc agccactgcc gccatcgccg cgccaataac aaaggtcaaa gcaatcaccc
    10081 ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc ttccgcgcag gcacggcagg
    10141 cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg aggaaagtaa
    10201 caatccagat cactgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    10261 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcggtcaggc
    10321 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    10381 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcaa
    10441 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    10501 tcatcagtgc ggcgatgatc ataaaggaga catagctacc aatcatgtaa acctcgccgt
    10561 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gctatcagcg
    10621 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    10681 cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg atgatcactt
    10741 ggctgccgtg gatgaaccat cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    10801 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    10861 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    10921 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    10981 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    11041 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    11101 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    11161 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    11221 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    11281 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    11341 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgttgg
    11401 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    11461 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    11521 tgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcattaac
    11581 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    11641 aaccagcttg tcgcccttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    11701 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    11761 gactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    11821 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    11881 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    11941 tgattaaggg tttagcgcag tattttgtga taacagtgat taaaatccct atttttcagt
    12001 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agtgcctttg
    12061 tcagcataaa ataacggcac aaagggcgga ataattcact accattcagg ggattatgct
    12121 gggtattttt cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca
    12181 cctgccaaaa gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc
    12241 cacttttgtg atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt
    12301 agcgaccagg accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta
    12361 caggttgacg ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc
    12421 gtacgggtaa ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc
    12481 acccgacgtc gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc
    12541 gtttcatgct ggtggatagc ggatgcaggc gtggaagatc gcggtgtgat gacagcgttt
    12601 atgcaggcac tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt
    12661 tgatttcaaa agtgatgacc aacgagaaat tgcctgatgc gctacgctta tcaggcctac
    12721 aatgtgtgtt gcaatttact gatttctttg gatcttatag gccggataag gcgtttacgc
    12781 cgcatccggc atgaagcaac gtactcgata ttagcaattt ggcggcaacc caaagttgcc
    12841 gattaatgat tacttcgcat ctgtcgccgt gccgttggcg tgccagtcaa atacgccgaa
    12901 ctcaaagcct ttcagatcgc ctttctcatc ccacgtcagc ggtcccatta cggtatccac
    12961 ggagttcgct ttcaggtatt tggcgatttc agccggatcg tcagactgat tcaggcccgc
    13021 ctgcaaagat tgcagcgcgg cgtaggtggt ccaaacgaat gcgccgcttg ggtcctgttt
    13081 tttcgctttg atcgcgtcaa caatgggttt gtttgccgga acctgatcgt agttcttcgg
    13141 cttggtcacc agcagccctt ccgctgattc gcccgcaatg ttagacagcg aaacgttagc
    13201 cacaccttcc ggccccataa actgagtttt cagccctgcc gcgcgtgcct gacgcaggat
    13261 ttgccccatt tccgggtggt aaccgccgta gtaaacgaag tcgatattct ctttcttcag
    13321 acgcgccacc agcgtagaga aatctttttc cccggcggtg ataccatcaa agaacaccac
    13381 gttagcattg cctttcttca ggccgtcctg caccgctcgt gccagacctt cgccgtattg
    13441 ctgtttgtcg tgaacgatag caatacgctg cggtttcact ttctcaagaa tatatttcgc
    13501 cgccgtcggc ccctggtcgg agtccaggcc ggtggtgcgc aggatcagct gatagccacg
    13561 ggcggtcagc tccggcgcgg ttgccgctgg ggtgatcatt aaaatgcctt cgtcttcgta
    13621 gatgtcagac gcaggctgcg ttgatgaaga acagaggtga ccaatcacat atttaatgcc
    13681 gtcgttaacg actttgttcg ccaccgcaac cgcctgtttc gggtcacagg catcgtcata
    13741 ttttacgatt tgcagtttgt tgcctttaat gccgccttta gcgttgatat ccgcaaccgc
    13801 ctgctctgcg ccggtaaact cttggtcacc gtactgcgca accggaccgg acattgcgcc
    13861 cacgaccgcg actttaatat cttctgccag agccatattg ctgaatgcca gcgcgataca
    13921 tcctgccagt aacgctttac cctttatgtt catcctgaga atccccattc ttctggttat
    13981 tacgtgtgtt gtgatgttgt tgttcagcac tttatttcgt tttatgcatg gctacccgtg
    14041 ctttagcagc atactctgct aaaacatacc cgatttttat gatattggaa tagctatttt
    14101 gacagtttat taacaatctg cgtggggatt ggcgttttgc cggaggggaa attgattatt
    14161 gtagaggccc aaaaaacaaa aaccccggac tcgcatccag ggttctctgc ttaatagcgg
    14221 aaattacgct tctatagcag cgcgcagttt tttcatcgcg ttcttttcca gctggcgtac
    14281 acgttcagcg gaaacaccgt aacggtcagc cagttcctgc aacgtggact tgttgtcttc
    14341 gtccagccag cgcgcgcgga tgatgtcctg gctgcgttcg tccagacctt gcatcgcgtc
    14401 ggtcagacgg tttgccgcct gctcttccca gttatcatct tcgatgccgt cggcaaagtt
    14461 agatgattta tcctgcagat agagcaccgg tgccatcggc tggctgtcgg aatcgtcgtc
    14521 ggaagacagg tcaaaggtca tgtcctgtgc cgccatacgt gattccatct cacgaacgtc
    14581 tttgctggtt acgcccagtt cacgggccac catttcgact tcatcctggt taaaccagcc
    14641 cagacgctgc ttggttttac gcaggttgaa gaacagtttg cgctgcgctt tggtggtcgc
    14701 aactttgacg atacgccagt tacgcagaac gtattcgtgg atctctgctt tgatccagtg
    14761 aacggcgaag gagaccaggc gcacacccac ttccgggttg aaacggcgca ctgctttcat
    14821 caggccgatg ttaccttcct gaatcaaatc cgcctgtggc aggccatagc ccgcataatt
    14881 acgagcaata tgaacaacaa accgcaggtg agacaggatc agcgttttag ctgcttccag
    14941 atcgccatgg taatgcagct tttcagccag cgcccgctcc tcgtcagccg acaacatcgg
    15001 ccacgcgtta gctgcccgga tgtaggaatc caggttgcca actggggcta aagctaaact
    15061 ttgcattttg tcagtcattc aaatcctctc aatcgatatc ttctggcgct tcagtggtag
    15121 caacaactgt gccagagctt aagagcaacg aggttatcat ccactgtttt atcagacagt
    15181 gattttatcc acaagttcaa tgcaagcttg tgaataaatt acgcacaaaa tgtgacatag
    15241 agatgaaata ccgggaagag acaacgggat ctctttccct gctacggaac ccattgcagg
    15301 gaaagagtat aacacgcttt tattattcag gcgtaaagtg gcgtaaatgt tgtaccgtgg
    15361 caagccacgc tgccacccag ccaatcatcg agcataccag cagcaatagc aggcattcat
    15421 cgaacgataa accattgata tcaaacttcg ttccgaaaac ctgtgccact tccgcaaccg
    15481 ccgatgacaa tcgcagcacc agaatttctg acaaaattaa tgacaacaat gcgccagaaa
    15541 atcccagcag tgcgccacca tacaggaacg ggcgaaggat gaaaccatcc gtcgcaccaa
    15601 tcagtttctg cacgttgatg gagtcacggc gagcaaagat gctcagacgt acgctgttgc
    15661 cgatgacgag gaacactgcc gccaccatca acacgccgat catcgccgaa acgcgcccga
    15721 ccagcccggt caacgccgcc agacgggcaa accagctgtc atccatccgc acttcgtcaa
    15781 tgccgttaat ctgcgtgata cgatcacgca gcgtattgag tgattccgtc ccctgaaaat
    15841 cgagtttcgg gatcaccacc gccactgccg gaagcgggtt ttcttccagc atatccagcg
    15901 caccaccaaa accagaccag ttacggaact cgcccagtgc gtcttcacga gaaagatagt
    15961 tcactttctc cacgccttgc tcggcctgca actgtgccac cacgcccgca gcggcgtcat
    16021 cgtccagcgt tttttgcaag taaacagtga tttgcggtga cggataatac tgcgtcgccg
    16081 cctgattaac gtttttgtac accatataac agacgctggg cagcgtcagg gagatggcga
    16141 taaccatcac cgttaaaaac gtggcgaacg gtttgctttt cagatcctgc aatgcgccgt
    16201 ggaaggcata gcgcacctgt tcgttgaaaa cgttggtttt gcgatttacc ggttttggcg
    16261 aggatttcgc gcgtttcggt gcgttacgac cgccgtcgcc tgagccgccg accgatttac
    16321 ggaagcgatc cagacgccca ccaaactgcc gaatatgatt gattgcatcg cgcttattca
    16381 tggcccacgc ctccatgcaa gtgaccatcg ctcagggtga gcatgcgatg ggaacgccgt
    16441 gaaatcaggt tgatgtcgtg cgttgccatc aatacggtta ccccaacgcg gttaaactct
    16501 tcaaacagac gtagaatccc ttccgacaac gcgtcgtcca ggttaccggt cggttcgtcc
    16561 gccagcagta ccgcgggctt attcaccacc gcgcgggcaa tgccaacacg ctgttgttca
    16621 ccgccggaaa gctgaatagg gaagttcttc gctttgtcca gtagcccgac tttatccagt
    16681 gccgccgaca cccggcgacg aatatcgtca ccgctggcac cagcgataat cagcgggatc
    16741 gccacgttat cgtagacagt acggtccatc agtagatggt gatcctggaa aatcatgcca
    16801 atctggcggc gcagaaacgg aacttcacgg tttttcagac gcgtgatgtc atggccgcta
    16861 aaccagattt tcccggcgct gggccgctca atcccacaga tcagcttcag gagggtactt
    16921 ttccctgcgc cggaatgacc ggtcagaaac gccatctcac ccggctgcat atggaacgta
    16981 acgccctgca gcgcctgtct cccaccgaga taagccttgc tgacatgttc aaagcgaatc
    17041 attgttaatc ctctcgggca aaaagtgcct ctataaagtc gtccgcctta aacggacgca
    17101 aatcctcaat acgttcgccg acaccaatgt agcggatagg gataccaaac tggtcagcca
    17161 ccgagaaaat taccccgcct ttcgccgtgc cgtccagttt cgttagcgtg atgccggtta
    17221 agccaacagc ttcgtggaac agtttggcct ggcttaccgc gttctgcccg gtgctggcat
    17281 caatagtcag cataacttca tgcggcgctt caacgtcgag tttcttcatc acgcggacga
    17341 ttttcttcaa ctcttccatc aggtgcgatt tgttctgcag gcgtccggcg gtatcggcaa
    17401 tcaggacgtc gatattacgc gctttagctg cctgaatggc gtcgaagata acagaggcgg
    17461 aatccgcccc ggtatgctgg gcaatcaccg gaatattgtt gcgctgaccc cagacctgaa
    17521 gctgttcaac cgctgctgca cggaaagtat cgcccgccgc cagcatcacc gatttaccct
    17581 gctgctcaaa ctgacgtgcc agcttaccaa tcgtcgtggt tttacccaca ccgttgacgc
    17641 ccaccatcag gatcacgaac ggcgttttac cttcaacatt cagcggctca tcgactttcg
    17701 ccagaatctc acccatctct tctttcagca ggccatagag cgcctcggca tcacgaagct
    17761 gcttgcggga tgcgccttcc gtcagattgg tgataatttt acgtgtggtt tccacgccca
    17821 catcggcgat caaaagctgc tcttccagct cctcaaacag atcatcgtcg atttttttac
    17881 cgcggaacag gctgataaat ccggaaccga gattttcttt ggtttttaac aggctgcgtt
    17941 tcaggcgcgc gaagaaacct tctttggttg gtttttcctg ctcctgagcg atttcttcca
    18001 ccggctgctc ttcttctgcc ggaggaacca ccatcaccgc ctcttctgct gcttcggctg
    18061 ccagcgcctg tgcttccagc tcttcgtcgg tgatttcttc tttagccgct tcttcttccg
    18121 ccgctttgac aatctctacg gtttccgctt cagcctgcca ctcttctggc gaaacctctt
    18181 cggcgttgac gtcttccggc aacggcagct cttcacgttc gatagccact ggctccggcg
    18241 tttcttctac gaccggttcc ggctgtgcaa caacttccgc ttcaggctgc gctttttcac
    18301 tttcaacaac ctgttcagtg acttccacaa cgtcggcagc aaaagtttcc gcctcggctt
    18361 cagtatgcgc ctgcggctgc tcttcaacgg cttgttccga agccttcaca ggctcttgcg
    18421 cctgaacgat ttcttctaca accggttgtt cattctgaac ttctgtctct ttttccgggg
    18481 tctgctcttt ttgaccaaag cccagccagg aaaaaaagcc acgttttttt tctttcgcca
    18541 tttgcgacta cactcctcgc tgttgcttca tggcacagcg ttaacgctat gtacatagca
    18601 gctaaaaaaa tgatgaaata gtctatcact taacttaatt cacatcaccg cctgcaagta
    18661 tgtgttatct ggcggattga gcaatttatc atgaaaaaac cgaatcattc cggcagcggc
    18721 caaatccgca ttattggcgg gcagtggcga ggccgtaaac tcccggttcc tgatagccca
    18781 ggtctgcgcc ccaccaccga ccgcgtacgc gaaacgttgt ttaactggct ggctccggtc
    18841 attgttgacg cccaatgtct ggattgcttc gccgggagcg gcgcgctggg gctggaagcg
    18901 ttatcgcgct acgctgcggg ggccacgtta attgagatgg atcgtgcagt ttctcagcag
    18961 ttaattaaga atctggcgac actaaaagca ggcaatgcac gcgtggtgaa cagcaacgcg
    19021 atgtcattcc tggcgcaaaa aggcacaccg cataatatcg tgtttgtcga tccaccgttc
    19081 cgccgtggct tgttagaaga gacgataaat ttactggaag ataacggctg gctggctgac
    19141 gaagccctga tttatgtcga aagcgaagtc gaaaacggtc tgcccaccgt tccggcaaac
    19201 tggtcgttac atcgggaaaa agtggcgggt caggtggctt atcggctgta tcaacgcgaa
    19261 gcacaaggag aaagtgatgc tgattaatat tggtcgtttg ttaatgctct gcgtttgggg
    19321 atttttaatc ctcaatctgg tgcatccctt cccgcgcccg ctaaatattt tcgttaacgt
    19381 ggcgctgatt tttaccgtgc tgatgcatgg tatgcagctg gcgctattga aatccacttt
    19441 accgaaagat ggcccgcaga tgaccaccgc cgaaaaggta cggattttcc ttttcggcgt
    19501 gtttgaactg ctggtctggc agaagaaatt taaagttaaa aaataacctt attgttcgcc
    19561 aacaaagcta acaaagcgcg ttcctttata gctcagcgta cctttatcgc ccactgtcag
    19621 ggcgtggtac tgctgggcgt cgaggcgaaa cgtctgctcc attcctccgc tttgcggttt
    19681 gaagcttgcc tcgtagcgca tactggtgcc tgccggagtc acttcctgct ggcgcgaacg
    19741 gcgatcgtta atcggttttt cccgcttgtt gctcaccacc accagctttt gctggagcgg
    19801 agccatatca ttatcagctt tttcccgtcg ctgttgcata aaacgaaacg atgcggcgac
    19861 gacaattaag ccaatgataa caataaagaa aagaggtggt ttgctcatct ttatccctca
    19921 tcggaaaatg cggaaataag cataccctgc cagttatggt gttgtcatcc gtccaccctc
    19981 gccactaaac tggaagcaag accgtaggca ttccgcttac gaaaaaataa cgaattcaag
    20041 gaactaagat gctttggtcg tttatcgctg tctgtctttc cgcatggcta tctgtggatg
    20101 catcgtatcg tgggccaacc tggcaacgct gggtgtttaa accgttaacc cttcttctcc
    20161 tgctgttact ggcctggcaa gcgccgatgt tcgacgccat tagctatctg gtgctggcag
    20221 ggctgtgcgc ctcactgctg ggcgatgcgc taaccctgtt gccacgtcaa cgtctgatgt
    20281 acgccatcgg cgcgtttttc ctctcgcacc tgctgtacac catctatttc gccagtcaga
    20341 tgacgctctc tttcttctgg cctctgccac tggtgttgct ggttctgggt gcgctgttac
    20401 tggcgattat ctggacgcgg ctggaagagt atcgttggcc tatctgcacg tttatcggca
    20461 tgacgctggt gatggtgtgg ctggcaggtg aactgtggtt cttccgtccg accgctccgg
    20521 cgctctctgc gtttgttggc gcttcgttgc tgtttatcag taactttgtc tggctgggga
    20581 gccactatcg ccgacgcttc cgtgcggata acgcgattgc tgcggcctgc tactttgccg
    20641 gtcacttcct catcatccgc tcactgtatc tctgataaaa cttgactctg gagtcgactc
    20701 cagagtgtat ccttcggtta atgagaaaaa acttaaccgg agtatgccat gtcgacacct
    20761 gacaatcacg gcaagaaagc ccctcaattt gctgcgttca aaccgctaac cacggtacag
    20821 aacgccaacg actgttgctg cgacggcgca tgctccagct cgccaacgct ctctgaaaac
    20881 gtctccggca cccgctatag ctggaaagtc agtggcatgg actgcgccgc ctgtgcgcgc
    20941 aaggtagaaa atgccgtgcg ccagcttgca ggtgtgaatc aggtgcaggt gttgttcgcc
    21001 accgaaaaac tggtggttga tgccgacaat gacattcgtg cacaagttga atctgcggtg
    21061 caaaaagcgg gctattccct gcgcgatgag caggccgccg acgagccaca agcatcgcgc
    21121 ctgaaagaga atctgccgct gattacacta atcgtgatga tggcaatcag ctggggtctg
    21181 gagcagttca atcatccgtt cgggcaactg gcgtttatcg cgaccacgct ggttgggctg
    21241 tacccgattg ctcgtcaggc attacggctg atcaaatccg gcagctactt cgccattgaa
    21301 accttaatga gcgtagccgc tattggtgca ctgtttattg gcgcaacggc tgaagctgcg
    21361 atggtgttgc tgctgttttt gattggtgaa cgactggaag gctgggccgc cagccgcgcg
    21421 cgtcagggcg ttagcgcgtt aatggcgctg aaaccagaaa ccgccacgcg cctgcgtaac
    21481 ggtgagcggg aagaggtggc gattaacagc ctgcggcctg gcgatgtgat tgaagtcgcc
    21541 gcaggtgggc gtttgcctgc cgacggtaaa ctgctctcac cgtttgccag ttttgatgaa
    21601 agcgccctga ccggagaatc cattccggtg gagcgcgcga cgggcgataa agttcctgca
    21661 ggagccacca gcgtagaccg tctggtaacg ctggaagtgc tgtcagaacc gggtgccagc
    21721 gccattgacc ggattctgaa actgatcgaa gaagccgaag agcgtcgcgc ccccattgag
    21781 cggtttatcg accgtttcag ccgtatctat acgcccgcga ttatggccgt cgctctgctg
    21841 gtgacgctgg tgccaccgct gctgtttgcc gccagctggc aggagtggat ttataaaggg
    21901 ctgacgctgc tgctgattgg ctgcccgtgt gcgttagtta tctcaacgcc tgcggcgatt
    21961 acctccgggc tggcggcggc agcgcgtcgt ggggcgttga ttaaaggcgg agcggcgctg
    22021 gaacaactag gtcgtgttac tcaggtggcg tttgataaaa ccggtacgct gaccgtcggt
    22081 aaaccgcgcg ttaccgcgat tcatccggca acgggtatta gtgaatctga actgctgaca
    22141 ctggcggcgg cggtcgagca aggcgcgacg catccactgg cgcaagccat cgtacgcgaa
    22201 gcacaggttg ctgaactcgc cattcccacc gccgaatcac agcgggcgct ggtcgggtct
    22261 ggcattgaag cgcaggttaa cggtgagcgc gtattgattt gcgctgccgg gaaacatccc
    22321 gctgatgcat ttgctggttt gattaatgaa ctggaaagcg ccgggcaaac ggttgtgctg
    22381 gtagtacgta atgatgacgt gctgggtatc attgcattgc aggataccct gcgcgccgat
    22441 gctgcaactg ccatcagtga actgaacgcg ctgggggtca aaggagtgat cctcaccggc
    22501 gataatccac gtgcagcggc ggcaatcgcc ggggagctgg ggctggagtt taaagcgggc
    22561 ctattgccgg aagataaagt caaagcggtg accaagctga atcaacatgc gcccctggcg
    22621 atggtcggtg atggtattaa cgacgcgcca gcgatgaaag ctgccgccat tgggattgca
    22681 atgggcagtg gcacagacgt ggcgctggaa accgctgatg cagcattaac ccataaccac
    22741 ctgcgcggcc tggtgcaaat gattgaactg gcacgcgcca ctcacgccaa tatccgccag
    22801 aacatcacca ttgcgctggg gctgaaaggg atcttcctcg tcaccacgct gttagggatg
    22861 accggcctgt ggctggcggt gctggcagat acgggtgcga cggtgctggt gacagcgaat
    22921 gcgttaagat tgttgcgcag gagataaggc aaaccgatcg caacattgag cgcgatcggt
    22981 cccctcgccc ctctggggag agggttaggg tgaggggaaa aggcggcatc gaagccaatc
    23041 tttccccatc aaccgccttt acgaatcaaa taacgataag gcagtccatc cgtctctttc
    23101 gcaaccagtt cgtgttccat aaaggtacaa aacccaggaa tatcgcgggt agtggccgga
    23161 tcgtcggcga taatcagcaa cgtttcgcca ggctgcatat tgcgcacagt tttgcgcacc
    23221 atcattacgg gttccgggca gcgcaggcca agtgcgtcga gtgtgtggtc agggctggaa
    23281 aagagatcgg tcattttctt ctcatcacat aaaaaaacgg cgctagttta cgccctgtga
    23341 gtccgtaagc aaaccaggtt aacgattgcg tgaaaattaa ccattgcatt gtcaacgtaa
    23401 agcagtatca tgcggcggct cgaaaaaagg gtaagcacgt tattatgtta aggtaacaga
    23461 cgtgtcgtac gtattgggtt ccctcacccc aatggttaat caaaaaggta caatatgaac
    23521 gttttctcgc aaactcaacg ctataaggcg ttgttctggt tatcgttatt tcatctgctg
    23581 gtgatcacct ccagtaacta tctggttcag cttcccgtct ccattttggg tttccatacc
    23641 acctggggcg cgtttagctt cccgtttatt ttccttgcta ccgacctgac cgtgcgtatt
    23701 tttggcgcac cgctggcccg tcgtatcatc tttgcggtga tgatcccggc gttgctgatc
    23761 tcctacgtca tctcgtcgct attctatatg ggttcctggc agggattcgg cgcactcgcc
    23821 cacttcaacc tgtttgtcgc ccgtatcgcc accgccagtt tcatggccta cgcgctggga
    23881 cagatcctcg acgtgcacgt ttttaaccgc ctgcgtcaga gtcgccgctg gtggctggca
    23941 ccgacagcgt ccacactgtt cggtaacgtc agcgacacgc tggccttttt cttcattgcc
    24001 ttctggcgta gcccggatgc ctttatggct gaacactgga tggaaatcgc gctggtcgat
    24061 tactgtttca aagtgttaat cagtatcgtt ttcttcctgc caatgtatgg cgtattactc
    24121 aatattctgt tgaaaagact ggcagataaa tccgaaatca acgctttgca ggcgagttaa
    24181 aggttcgtta tcagagttgt gataagatgg atgaatgagc cgttatggcc gtttatcgaa
    24241 aggaagaagt caatgcgcaa tctggttaaa tatgtcggaa ttggcctgct ggttatgggg
    24301 cttgcggcct gtgatgataa agacactaac gctacggcgc agggttcggt cgcggaaagt
    24361 aacgctaccg ggaatcccgt caacctgctt gatggcaagt taagtttctc gctgccagcg
    24421 gatatgaccg accagagcgg taagctggga acgcaggcca ataacatgca tgtctggtcc
    24481 gacgccaccg ggcagaaagc agtcatcgtc atcatgggcg atgatccgaa agaagatctg
    24541 gcggtgttgg cgaagcgtct ggaagatcag caacgtagcc gcgatccgca gctgcaagtg
    24601 gtaaccaaca aagccattga gctgaaaggt cacaaaatgc agcagttaga cagtattatc
    24661 tccgcgaaag gccagacggc gtactcttcc gttattctgg gtaacgtggg taatcaactg
    24721 ctgaccatgc aaattactct gccagctgac gatcagcaaa aagcgcagac caccgcagaa
    24781 aacatcatta atacgctggt tattcagtaa gttttaagat gatgaggcgg cctcagggac
    24841 gtgttccgga ggccgttttt ttaatcgcca cgtcagtaat aacgcgattg cgaccagtcc
    24901 cgccgccgcc agataaatca ccggtacgcc cgcccagctc atcaccagcc cagccagtgg
    24961 tccggtcacg ccaagcgata aatccataaa tacggtgtaa gttgccagcg ccgccccctg
    25021 attttgctgc ggaaccgctt ttaccgccac tacgcccaat gccgggaaca ccagcgaaaa
    25081 ccctgccccc gccagtaaga cgccgacttt cgccatccac ggcatagtcg ccacgccaac
    25141 cagtagcagg ccgattatct caacgctaaa gcaaatcatc gccacgttta agccgccgat
    25201 acggttaatg ccgttaggga ataacaaacg cgtaccgaca aacgcacagc taaacagcgt
    25261 cagcgcgaaa gccgcaccgt cccaaccttt agcgtcataa aacagcgtga taaaggtggc
    25321 gatgacgcca aatccggcag aagccagtgc cagtgccata ccgtacagcc agacgcgccc
    25381 aagcaccgcg cgaaacggca gcggtttgcc tttactggct tttaccgtcg gacgcgggat
    25441 cgccaacaaa atggccacca gcgccacgcc cataatgatt aacgctaacg cctgcaagcc
    25501 gccccagtga taaaacacga cgcctaacgg cgcacccatc gccatcgccc cgtaattgac
    25561 aatgccgttc cacgaaatca ctcgcccgat atgcagcgag ccaaccacgc caacgcccca
    25621 cagggtcgat cccgttccgg caaaactttg cccaatccca aggatcacgc gtcccaggca
    25681 aagtaataac aggctgatga cgggcagact ggcggttaat cctgccgtca gataccccag
    25741 accgctcaaa aagcagccgc ataaaccgaa gacgacaatc tttttggggc ccagcaaatc
    25801 ggcgtaacgt ccggcatgag gacggctcag caaggtggcg aaatattgca ggctgataac
    25861 caatcctgcc cagaaagcgc taaagcccat cacatcatgg acatagcccg gtaatacagc
    25921 gagcggcaac ccgatggtga ggtagctggc gaagttaaac atgacaatgg agacaatgcg
    25981 caaattcagg cgcaatccgt ttagcgcggg ttcggctacg ggttcgggca tgaggatcac
    26041 cacattttta caacagtgtt tcatttttac cacgtgctga cgtgaaaatc agcagtaaga
    26101 atcagaatat tgctggcgtg actcccgcta cacttaatac aaaaagtcac aaggaagccc
    26161 caatggaaat ccctcaaccc gataaaacgg gcatgcacat tctgctcaag ctggcctcgc
    26221 tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag ctgttactgg
    26281 cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt cggggagtac
    26341 aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta accgcgctgg
    26401 tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag tttaataagg
    26461 agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat ttgcatatgt
    26521 cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc accacagcgc
    26581 taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg accgtagttt
    26641 ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg aataacccac
    26701 agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcactat cttgcattga
    26761 agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggag ctgatggggg
    26821 tgcagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg cccaatatcg
    26881 gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat ggtgtttacg
    26941 aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc aatattttag
    27001 aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt ctttcattgt
    27061 taatttgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca ttaaccagtg
    27121 tgtgtaaaat ctggatggaa accaccaaag gcggtagcaa actggcgatt ttactggggc
    27181 caggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat acgcactttc
    27241 attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa gtttcgacct
    27301 taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt ccacgacgcg
    27361 aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta ccggagatca
    27421 tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg ttcaacttaa
    27481 gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc ggctgcgata
    27541 tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg ttcagcctcg
    27601 gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg ttctggcgca
    27661 tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg caaatcgtca
    27721 gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta gaaaatttaa
    27781 gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg caatggatcg
    27841 attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac aggtaatcag
    27901 tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat ccattggggt
    27961 taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg cgtcttttat
    28021 cgtccatgcc gctgcaccag atgaaatcac taccgcctgg ccggtgaatg tcgggccact
    28081 aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt atgaaccatt
    28141 ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct ggactcattc
    28201 agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct ccaacggtga
    28261 accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata accgtcaacg
    28321 tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca gtaaaacaga
    28381 gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg ccctgccccg
    28441 tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga acggaattaa
    28501 agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc agtacgatgt
    28561 cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga tcacctttaa
    28621 cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata tcgacctgct
    28681 gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc agaatccggc
    28741 ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca ataccgccaa
    28801 agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa acaaaaaatc
    28861 gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt ttgccccttc
    28921 tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga aagcgaaaga
    28981 gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg agaaaaatgg
    29041 tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca aatcgatggc
    29101 ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga ttggcgaaga
    29161 agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt tccaccgcac
    29221 ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac cgtcgcacgc
    29281 tgacttccag gcacagcaag gattagccga caagccgctg attgataaag agatcggcga
    29341 agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca ttttgacccg
    29401 tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg tggtatcaaa
    29461 accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt tcgaacagat
    29521 taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct gctgatcccg
    29581 atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac cggcgacccg
    29641 gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct ggcctctacc
    29701 cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg gttgtggaag
    29761 gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtgct ggacgatatg
    29821 ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt aattctgctc
    29881 acttccgtac cgctcggtat ctgggcggcg cgccatcgcg accgtctgcc ggatttcgcc
    29941 gtacgtttca tcgcgtttct tggcgtgtcg atgcctaact tctggctggc gtttttactg
    30001 gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggttacgg cggctggcag
    30061 cacatcattt tgcctgcggt ttccattgcc tttatgtcgc tggcgattaa cgcgcgactg
    30121 ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacttgggc gcgtctgcgc
    30181 ggcctgaacg acaaacagac cgaacgccgc catattctgc gcaatgcctc gctgccgatg
    30241 atcaccgccg tggggatgca catcggcgaa ctgattggcg ggacgatgat tatcgaaaac
    30301 atctttgcct ggccgggcgt cgggcgctat gcggtgtcgg cgatttttaa ccgtgactat
    30361 ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg taatttgatt
    30421 gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg agcgcacgcg
    30481 tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt atcgccctgc
    30541 tggcgctgat tgcgctcacc agccagtggt ggctgccgta tgacccccag gcgattgatt
    30601 tgccgtcgcg cctgctttcg ccggatgcgc agcactggct gggcaccgat cacttaggtc
    30661 gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg gtaatggcct
    30721 gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg ttgattggcg
    30781 ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc ccgacctcga
    30841 ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta attatcgcca
    30901 tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg atttcactac
    30961 gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg cgggtgtttg
    31021 tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg ctggatatcg
    31081 gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg accgcgccga
    31141 ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc cagccgctgc
    31201 aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac ctggtgggtg
    31261 acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac taatgccgca
    31321 acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac acggtgtatc
    31381 gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca gcgggaaatc
    31441 attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga cggcggggga
    31501 aattttagcc gatggcaaag cggtttatcc ctgcgccctg cgcggcatca aaattgccac
    31561 catcatgcag aacccgcgca gcgcctttaa tccgctacac accatgcaca cccacgcgcg
    31621 ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg ctgccataga
    31681 agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg agatgagcgg
    31741 cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac cgtttatcat
    31801 cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc tcgatctgct
    31861 ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg atatgggcgt
    31921 tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg tcgaacaggg
    31981 cgatgtagaa acgctgttta acgcccccaa acatgcggta acgcgcagcc tggtttccgc
    32041 tcatctcgcc ctctacggta tggagctggc atcatgactt tacttaacgt ctccggcctt
    32101 tcccatcact atgcccacgg tggatttaac ggaaaacatc aacatcaggc ggtgctgaat
    32161 aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctggggcg cagcggctgc
    32221 gggaaaagta ccctcgcgcg gttgctggtg ggtttagaat cgcccgcgca ggggaatatt
    32281 agctggcgtg gcgaaccgct ggcgaaactc aatcgcgccc agcgtaaagc gttccgccgc
    32341 gatattcaga tggtatttca ggactccatc agcgccgtga atccgcgcaa aaccgtgcgc
    32401 gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga acaactggcg
    32461 cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga caaacgccca
    32521 ccgcagttaa gcggcggcca gctccagcgc gtctgcctgg ctcgcgcgct ggcggtcgaa
    32581 ccgaaactac tgattctgga tgaagccgtt tctaaccttg atctcgtgtt acaggcgggc
    32641 gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ccgcctgcct gttcatcacc
    32701 cacgacttac gcctggtgga acgcttctgt cagcgggtga tggtgatgga caacggacaa
    32761 atcgtcgaaa cccaggtggt gggagataaa ttaacctttt cctctgacgc cggacgtgtg
    32821 ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcaccacaga aaaggtttaa
    32881 cgcaaatgca acgagtcacc atcacgcttg atgacgattt actggagacg ctggacagcc
    32941 tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt ctgcgtagcg
    33001 ccctggccca ggaggccact cagcagcacg gcacgcaagg tttcgcggtg ctctcctatg
    33061 tgtatgaaca cgaaaaacgc gacttagcca gccgcattgt ctccacccag caccatcacc
    33121 acgatctctc cgtcgccacg ctgcatgtgc atatcaatca cgacgactgt ctggaaatcg
    33181 ccgtgctgaa aggtgatatg ggcgacgtgc agcattttgc cgatgacgtt atcgcccagc
    33241 gcggcgtgcg gcatgggcat ttgcagtgct tgccgaagga agattgagtt atttctgaaa
    33301 cgaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag ttgcatagat
    33361 agatcctgta aaggcagata ttctgcgtaa tgtacgccgc taaaatttat aaatgattca
    33421 aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc tgcgcagccg
    33481 cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc ttaatctttt
    33541 ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac tctcgcaggg
    33601 aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca caataacctg
    33661 acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta ataacagctg
    33721 gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt ttacccctat
    33781 acttagtaac cagtcacgca cgctttctac agcgccttta tcggtatctg gcgacggatg
    33841 aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttacaacgcg aacctcttcc
    33901 ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc acttgatccc
    33961 ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttttcttc cgtaaagcct
    34021 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcaa tagtatgaca
    34081 tcacagtatg aagtcaagtt tcccttcctt acgccattgt cccaatcgtc ttcctgaatc
    34141 gcagcagcgc aatggtgaaa aacgcgccgc caattgccat cagcgtcagg aactgcggcc
    34201 agacgatttc gaatccggca ccccggtaga gaatggcctg cgcgaggcta acaaagtgtg
    34261 tcgtcggcat ggtcagcata atgtcctgca ccatctgcgg catactttcg cgcggcgtgg
    34321 atccaccgga aagcatttgc agcggcagca gcaccagaat caccagcagc cccagttgcg
    34381 gcattgaacg cgctatcgtc cccataaaaa tgccgattga cgtggtggca aacagactga
    34441 gcgccacgcc cagcataaac agcgggatcg agccttcaat cggtacgccc agtacacctt
    34501 tcgccatcag caccagcgat aatcccgata ccaccagcac caccagcccc atcgaccaga
    34561 tcttcgccat catgatctca aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc
    34621 cgtgttcacg ctcgcggatc agcgccgatc cggtcaatac aatcgccagc atggtaatgt
    34681 tgttgatgat cgccatcacc ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca
    34741 tccgggtttc cagcgatacc aacggttcgc tgttatcacg gtagcgcgcg acaaagctgt
    34801 tcacttcacc gttgataata ttctggatat acccattgcc ggtaaatgcc tggctcatgc
    34861 gcgtggcatc gacgttcacc tgaatatccg gctggcgtcc ggcgaggaca tcacgctgaa
    34921 aattaggcgg aatatttatc gcgaaggtat agcgtccggc gtccagtccg gcatccatct
    34981 catcggcggt gatcatctcc ggtggcaaaa accacggacg atagaagctg ttaacgatcc
    35041 ggttcgataa ctgcgattga tccatatcgg caatggcgat cggcgcgagg ttcaacgatc
    35101 ctggcgtaac ggtcgctgac gaatacaccg acaccgtaaa cgagaagaca atcagcgtca
    35161 gcatcgcttt atcaccgagc agactgcgca actctttgat acccagatta aaaatattgc
    35221 gtaaatggcg catcatccct cctgtttttt cagcagcagg atgcttaagc ccatcaccag
    35281 cgggatggct atcagcaacg ggataaaaag ttgccacaaa tcagtcagat ccagcgcttt
    35341 cgagaacgtt ccgcgggcga tagtcagaaa atgactggtc gggtaaacct cgccgatcca
    35401 acgtccaggc ccttccagcg aagctaccgg atcgatcatc ccggaaaact gtgtcgccgg
    35461 gatcaacgtg ataatcgccg ttccgaaaat ggcggcaatc tggcttttca taaaggtgga
    35521 gatcagcagc cccattccgg tggcaatgat gatatacagc agcgccgcca gggtgagcgt
    35581 caggaaactg cctttatgcg gtacgccaaa cacaaacacc gacaggccgc agagcaggaa
    35641 aaagttcagc atccccagcg cgatgtacgg cagctgtttg ccaagcaaaa attcgctacg
    35701 ggtggtgggg gtcacgtaaa ggttgataat cgaccctagc tctttttccc gcaccacgct
    35761 aagggcgctt agcattgacg ggatcatcat cagcagaagc gggatcaccg ccggaacaat
    35821 cgctggcagg ctttttacgt ccgggttata gcgatagcgc gtctcaatgt tcatcagccc
    35881 gctttggctg gcgggtgtcg attgtcggct cgccacatcc tgaagccaac tctggtgcat
    35941 tgcctgcacg taacctttta ccgtttcggc acgactgggc atcgctccgt cgatccagac
    36001 gccgagttcc acaggcgtac cacgcgcgat atcgcgcccg aaattgggcg ggatctcaat
    36061 cgccaccgtg atatcgcccg cacgcatccg acgatcaagc tcgtcataac tggtgagcgg
    36121 cggctgttcg ataaagtaac gggaaccgga gaggttgagt gtccacgcct ggctactgac
    36181 ggtctggtcg cggtcgagca ccgcaaagcg caggttttcc acatccatac tgatgccgta
    36241 acccattatc agcatcagga tcaccgttcc catcagcgcc agcgtcgaac gtactggatc
    36301 gcgtcgcagt tccagcgctt cgcggcggct gtagctaaac agacggcgca ggctaaatcc
    36361 ctgacgcggc gcgtgggtgg tgtcgtgtac cacgggcggc gcttcggctt cgttgctctg
    36421 ccctgccgct tcctgcaaat aggcgataaa tgcctcttcc agactggcgg ctccgcgttt
    36481 ctcaaccagt tcctgcggtg taccgctggc aagcactttt ccggcgtgca tcagtgagat
    36541 gcggtcgcaa cgttccgctt cgttcataaa gtgggtagag atgaagatag tcactttgtc
    36601 ctggcgcgag agatcgacca tcaactgcca gaacatatcc ctcgccaccg gatcgacacc
    36661 agaagtaggc tcatcgagga ttaacatctc cgggcgatga atcaccgcca ccgccagcga
    36721 aagccgctgg cgaatgccga gcggcaatga ctccggcaga acatcttcaa cgtcgttgag
    36781 cttaaaacgc tcgctcattt cagccactct tgcgggaatt tccgcttccg ggatgtgaaa
    36841 caaacgggca tgtaactcaa ggttttgccg cacggtgagt tcgttataga gcgaaaacgc
    36901 ctgcgacata tagcccaccc gacggcgggt atcgatatct tttggatcaa ccggttgccc
    36961 gaacagccac gcctcacctt cgctggcggg cagcagtccg gtgagcattt tcatggtggt
    37021 ggatttaccg cagccgttcg aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa
    37081 attaacgtga tcaacggcaa cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat
    37141 ggcaatctct gcgttttcag gttgatacgg tgggatcact accgcctgat gcgcctggcg
    37201 ttgcgcttgc ggtaacagat ttataaatgc ttcttccagc gtagcgcttt gcgtttgctg
    37261 ccgtagctct tcggcgctgc cagttgccag cacttctccg gcattcatcg ctaccagcca
    37321 gtcgaagcgt tcggcctctt ccatataggc ggtggcgacc agcacgctca tattgctctg
    37381 ccgctggcga atactgtcga tcagatccca gaactgggag cgggagagcg ggtcaacccc
    37441 cgttgttggc tcatcaagga tcaacagttc cgggtcgtgg attaacgcgc agcacagccc
    37501 aagtttttgc ttcatcccgc cggagagttt ccctgccgga cgatcgcgaa acggtgctaa
    37561 ccccgtgctg gtcagcagct cattaattcg cacttcccgc tccgctttgt cgtgaccgaa
    37621 caggcgagcg aaaaaatcga cgttttcata caccgacaag gtgtggtaga ggtttttgcc
    37681 cagcccctgc ggcatccagg cgatgcgcgg gcagacgtcg cggcgatgcc tcgggtcgcg
    37741 catatcgccg cccagcacca tcacattgcc ctgctcaatg acgcgggcac cggaaatcaa
    37801 cgacaataag ctcgacttcc cgacgccatc cggaccaatc agcccgacca tacagcgggc
    37861 cggaatatcg agagtaatat tgttcagcgc aacgtttttt ccataatgct ggctcacgcc
    37921 cgccagttgc gcgacaggcg ggacgggaac cagttccaga tgcgtcattg cggcaacctc
    37981 accacgaggt cgtcaggcca cggaagttct tcattcaccc gcacccacgc tacgcccggt
    38041 aaaccggttt tgacatattc cagatgctgc tggagtaatt ccggtgggat acgcgctttg
    38101 acgcggaaca tcagtttcag ccgttcatcg ctggtttcga cggtttttgg cgtgaactgg
    38161 gcgacactgg cgacaaaact gatggtcgcc ggaatacgca gatctggcgc ggcatcgagg
    38221 atcagccggg cttcaccgcc aagtttcagc gtgcccgcct gttcggttgg caggaagaaa
    38281 gtcatataga cgtcgctgag atcgaccata ttcagcaccc gaccgcctgc cgccagcact
    38341 tcccctggct cagcaacccg atactgcacg cgtccgtcac gcggggcttt cagttcactg
    38401 tcatcgatat ctgcggcaat acgccgttca gtggcttgcg cggcttcgac gcgagtttgg
    38461 gcctgaatga tattggtacg ggccgcttct atagccgctt tagaagccga tacctgagct
    38521 tttgccgatt ccagcgcagc tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt
    38581 tgcgcagaaa tagcccctcg ttgggcgagc gagcgggaac gcgtatgacg tttagccacg
    38641 gagtccagtt cggactggcg ttgattaacc agcgactgcg cggcacgagt ttcgctttgc
    38701 cgttgctcca gcaaagcctg cgcggcagca acggcacttt gtgcttcttt gatttgcgcg
    38761 atggcttcca gtcgctgctc ctgcaacacg cgagtatcca tcttcgccag cacttcgcct
    38821 tcgcgaacaa actggccttc tttcaccaga atggtgtcga tacgcccggc aattttgctg
    38881 gcgatatcca cttccgtcgc ttcaatgcgc ccattgctga cagcaaagcc ttccggcaca
    38941 cccgccgggc gcaacagcca ccacgccacg acggcagcca ccaccagtaa cccgacaacc
    39001 caccacgcca gatggcgctt actcttatcc ataatcgacc cgccataatc cctgcaagca
    39061 aacgacgcgt gttacaactg ccgccgaacg acgttcagcg gattcttcca tccgggataa
    39121 gaaggaaacg acaaatacga ccagcgcgca gagcattgag cgccaacggg gaaggcttag
    39181 tgaaaacggg tagtaatgtt gtcatcatca gcattcctgg ccgtaaatga aacacgcata
    39241 ctcataagtg tgatctataa aactggctga caaaacggta tccatcctgt tggatagcgt
    39301 ttcacgcctg ttcagttgca ggaacagagc attccttttt acgttttatc aacagtctca
    39361 accagatagt aacattaata ttcatgttgt ctatggttca gagctgtaac attgtgcgcc
    39421 ttctgatcaa aggcataagt ccatttctgt ttttttcatg ctggtaagac ttccagggag
    39481 tggttatctc aggccctgca ccagggttaa tttggcaatt atgcatgctg aacattcttc
    39541 cagcatgttt actgtcacgg gcgttaagac ctaaaactgt ccccgtttca cctatacatt
    39601 ctcttccacc attaccgtta tgtgcaacaa cttgatttat acctaacatt tgtgagcatg
    39661 ctaatagtgc ttcaagttta ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg
    39721 atcgacaaga gatagcccgg ctatttttaa actttttgcc attaattaca gcatggtgct
    39781 ttttattgat aattgccgct aattcgaccg gatcgagagc attggtcatt tcatcactag
    39841 agactgttat cggtcctata atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat
    39901 gatttgccat tattttgtat ttggggtcat agaaacatac aggataatct tttatcccag
    39961 agtaggtatc ctcatcaagt ggtttcatgc gcgtgaagtt ctgcatatag ttaccattgc
    40021 aattcgtttc atgatttcca gctaatacat gaacgttttc attgatactc attatgcgtt
    40081 ttaataaatc gatgataaat ttatcgccac agatattact aaatcgatcg ccagtctggt
    40141 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aaccaaataa ggctgaagac
    40201 tttctaaaac aatgtctctt ctgttcagga tactgattat tatattaaca ttagtgtcaa
    40261 gttgctcatt aaagttctcc cttagcaata cgttgagaac ttcatcttct atattaagca
    40321 actggctaag gaaattttct ccttcagcca tgattcccaa atgtcccgtt gcaaaaagcg
    40381 cgtacaaaac tgcaccgact gagccatcgg tatcgctaaa ataggtcggt ctggtacttg
    40441 tgggatcaac aggtgaacaa ttggagaaat aggttctgtc agcgaactgg ggttttatca
    40501 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
    40561 tgtacgactc agtaactgca acatcattag cattatcatt tattgtcata ttattaagta
    40621 aataaacaat gctgcaccaa ttaatatcgg tattatcaag agggcaaccg taatttttac
    40681 tattaattgt cacaataaac tgataacagt tattcctacg tagaatatga gcgcgcatgc
    40741 ccgatagcat actatatttt tctgaatgat tcgtttgtat ggtaaatgaa attttgtcat
    40801 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
    40861 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
    40921 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gtgattgtcg
    40981 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattta
    41041 ccgcgaggaa tacatagtta acatttaatc ttatgacata cacctcatgt tcgatgggaa
    41101 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatctc
    41161 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgcct ctacaatcga
    41221 ttaagaaaac ctatgaaata ctggcaaaat taaaaagaga taccacagca ctcatgaagt
    41281 tttattgagg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
    41341 gtgctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
    41401 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
    41461 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattt tcatatacag
    41521 gtgtggatgc tacagcattg cttctaatta gatggataag acgatcatcc atgtgttgtt
    41581 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
    41641 taaatggaac ctgcaaagca ccgagacaat aacattcatt ttcttcattt cttattattc
    41701 cgtgatgact ggttaaaact ttcgtttctg agtcataatg acaaacatcg agcgttttaa
    41761 ttaagttata cgtatcaccg gcagagagtt tataattttc tagtcgtgca gtatagttac
    41821 cattaaaatt gatttcatga tttccagcca gaacgatgac atttttatta atacggctat
    41881 ctttattacc atccatattt cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
    41941 aaatagtact aaatctatcg cctgtatgat ccccaaggaa gatacaaggg gttatcgcat
    42001 cgtcctttaa aaaaacatgt tgatgcaact cattcaatat catgtctctc gtatttaata
    42061 aattgtagag caatttatta tttttatgta tatatgcaga tgaagccatg ttttcgtgtt
    42121 ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc cctgaaacga
    42181 gcaatgcata aagtacagcg cctactcttc catctgtatc gccaaaataa gccgtgttgg
    42241 cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa aacacagatt
    42301 taacatagct atcaaactta tccttaggaa acgtctcttt agagacatcc caacgccagt
    42361 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaagcccat
    42421 taactttata agttatgtct gatttataaa ttatgtctga tttatctccg gagaaattaa
    42481 taaaataata accatcctta cactcaatct taactgtaat gggagacgat aattttatca
    42541 catggttatt gtctggtata attgtaaaga taaacgtgtt agtagtatta tcggcgaaac
    42601 tattatattc ttgaatatct atagatggct cactattgta aaaccatatt ttctccatct
    42661 gaagactatg ttcatttacc ctccagtgac catgttgcag gaaatagatg atattcaggc
    42721 ctaaatcgtt atctatttgc gaaaagtcat tcagcatatt aacttcaact gtgtttgatc
    42781 gatatttttt atcaaccttg ccatggtgtt gatgactgtt aactttatct gaatgcttat
    42841 cttgatggtt tgaagagaag tgcttaaata tattgtgaaa tactttcata aataacctgt
    42901 aatattttta cttgttagta acaaaataaa caatacatgt ctgaaagaca tatttacgat
    42961 gatacaaagc aataatactt atatgttcga tgattttaag caccgagact atccggtctt
    43021 ttactaaata atggaaaaca atccatccct ggaagataat tgtaaaaaaa aacaacagaa
    43081 taagaattaa tcttaggata aatatttatt tatcatggcc ttttaaccgg taaaaaatgg
    43141 cactcggcac agtaaacccc gctatagtta tgctatgcat ttgtctttaa tgatatctcg
    43201 tcgttatatt aacaggctga aattatcatt aatgcacatt tcattattag cagggatacc
    43261 tgaggagttt aaagtgaaca tatatatcgg gtggcttttc aaattaatcc ctttgattat
    43321 gggattaatt tgcatcgcgt taggtggctt tgtgctggaa agttcagggc aaagcgagta
    43381 tttcgtcgcg ggtcatgtgc tgatttctct ggcggccata tgcctggcat tattcactac
    43441 cgcatttatt atcatttcgc agctcacgcg cggcgttaat acgttttaca atacattgtt
    43501 ccccattatt ggctatgcgg ggtcaattat caccatgata tggggttggg cactgttagc
    43561 aggcaatgat gtgatggcag acgagtttgt cgccggccat gttattttcg gcgttggtat
    43621 gattgccgcc tgtgtatcga cggtggcagc gtcatccggt cactttctgc tcattcccaa
    43681 aaatgcagcg gggagcaaga gcgatggaac gccggtacag gcttattctt cattaatcgg
    43741 taattgcctc attgccgttc ccgttttact caccctgctc ggtttcattt ggtctattac
    43801 gctgttacgt agtgctgaca taactccgca ttatgtcgcg ggtcacgtat tgcttgggtt
    43861 aaccgcaatc tgtgcctgtc taattggcct tgttgccaca attgtccatc aaacacgtaa
    43921 tacgttttca actaaagaac actggctgtg gtgttattgg gttatttttc tcggctcaat
    43981 cacggtactg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    44041 cggcattatt cttatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    44101 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgatacccgt
    44161 cttcacctgc ctgttttgcc ttttcctggc atcatttctt gcggaaatgg cgcagaccga
    44221 catgggatat tttattcctt cgcgagttct ggtcggtttg ggagcggtat gctttacgtt
    44281 gttctcaatc gtttcaatat tagaagcggg ttctgctaaa aaataattgc aacgtaccgg
    44341 ataaaaccag cgttgaccat ttgcgtaacg ctggtttttc ttaggcatca tgaaataacg
    44401 cagcattaat gcatagtggt taaggataaa aaaacaacaa agttactgtc tttttcagcc
    44461 tgttcatatt ttataaagat ctggccactg ggtgagtttt caatcacctt tccttccacc
    44521 ttatattaag cctggagggt ttcagttcac gggctcatta gaaaataatc acaagatagc
    44581 cactatcaaa attagctcat ttttaatgcg gctctaataa ttttcatctt taggtaacag
    44641 gtcaggacga cttcgctgca atcaaatcct gcgcacaagc ccacgcactc gaccacgccc
    44701 actggaagtt atagcccccc agccagccgg taacgtccat cacttcgccg atgaagtaca
    44761 gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag ttcgttggtg tccacgccgc
    44821 cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt gggttgtacg cgccagtcgg
    44881 tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag ctgttttagc gaaacattcg
    44941 ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt cggtagatga accgccagtg
    45001 tgtttttcag gctctgattc ggatgtgcgt tacgctgctc attcaggaag gtttcgagat
    45061 ccacatccgg tagcagattg atactgacaa attcacccgg ttgccagtag ctggaaatct
    45121 gcaacaccgc cggtccagac aagccgcgat gggtgaagag taagttctca cggaaaacga
    45181 tgccgttttc agcggtaatc acggaaggca ccgccacgcc cgccagcacc tgtaactctt
    45241 cgagcaacgg tttatgcaga gtgaatggca ccagacccgc acgggtcggc agcacgttga
    45301 ggccaaattg ttcggcaatc ttataaccaa acggcgacgc gcccagcccc ggcattgaca
    45361 gcccaccggt cgccatgacc agcttttcgc aaccgacagt catgccgttc agatcaagcg
    45421 tgaagcctgt ttcatcctcc gccacactca gcacttcgct acgcaatctg aaggtcacat
    45481 tgcccttctc gcactcatcc accagcatgt cgacaatctg ctgcgcggag tcatcgcaga
    45541 agagttgccc taacgttttc tcgtgccatg cgatgccgtg tttattgacc agatcaatga
    45601 aatcccactg ggtaaaacgt gcgagtgcag acttacaaaa atgcggattc tggctcagat
    45661 aagcgcctgg ttcgacataa aggttggtaa agttgcagcg cccaccgcca gacataagga
    45721 ttttgcgccc tggtttttta ccattatcga tcagcagaac ccggcgtcct gcctgacctg
    45781 ccagcgcaga acagaacata cccgccgcac cagcgcctat aataatggca tcaaaccttt
    45841 ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt aaagattcct cagtggtcgc
    45901 accagcatca atattactaa aaggaagtat ttgcctgaat tatataagat aattattttt
    45961 tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta tatttcactt tgcccgcgcc
    46021 gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa atggcgtaac gtcctatgct
    46081 acatttgttt gctggcctgg atttgcatac cgggctgtta ttattgcttg cactggcttt
    46141 tgtgctgttc tacgaagcca tcaatggttt ccatgacaca gccaacgccg tggcaaccgt
    46201 tatctatacc cgcgcgatgc gttctcagct cgccgtggtt atggcggcgg tgttcaactt
    46261 tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc attgtgcata tgctgccgac
    46321 ggatctgctg cttaatatgg gatcgtctca tggccttgcc atggtgttct ctatgttgct
    46381 ggcggcgatt atctggaacc tgggtacctg gtactttggt ttacctgcat ccagctctca
    46441 tacgctgatt ggcgcgatca tcgggattgg tttaaccaat gcgttgatga ccgggacgtc
    46501 agtggtggat gcactcaata tcccgaaagt attaagtatt ttcggttctc tgatcgtttc
    46561 ccctattgtc ggcctggtgt ttgctggcgg tctgattttc ttgctgcgtc gctactggag
    46621 cggcaccaag aaacgcgccc gtatccacct gaccccagcg gagcgtgaaa agaaagacgg
    46681 caagaaaaag ccgccgttct ggacacgtat cgcgctgatc ctttccgcta tcggcgtggc
    46741 gttttcgcac ggcgcgaacg atggtcagaa aggcattggt ctggttatgt tggtattgat
    46801 tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc actggctacg aaatcacccg
    46861 tacccgtgat gccatcaaca acgtcgaagc ttactttgag cagcatcctg cgctgctcaa
    46921 acaggctacc ggtgctgatc agttagtacc ggctccggaa gctggcgcaa cgcaacctgc
    46981 ggagttccac tgccatccgt cgaataccat taacgcgctc aaccgcctga aaggtatgtt
    47041 gaccaccgat gtggaaagct acgacaagct gtcgcttgat caacgtagcc agatgcgccg
    47101 cattatgctg tgcgtttctg acactatcga caaagtggtg aagatgcctg gcgtgagtgc
    47161 tgacgatcag cgcctgttga agaaactgaa gtccgacatg cttagcacca tcgagtatgc
    47221 accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt atcggtacga tgattggctg
    47281 gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag aaaggcatga cctacgctca
    47341 ggggatgtct gcccagatga cggcggcagt gtctatcggc ctggcgagtt ataccgggat
    47401 gccggtttcc actactcacg tactctcctc ttctgtcgcg gggacgatgg tggtagatgg
    47461 cggcggctta cagcgtaaaa ccgtaaccag tattctgatg gcctgggtgt ttacccttcc
    47521 ggctgcggta ctgctttccg gcgggctgta ctggctctcc ttgcagttcc tgtaatcgta
    47581 cgcaccaaaa cgagcgggtc agctggcccg cttcagattg tgacatagtg cgctttgttc
    47641 atgccggatg cggcgtgaac gccttatccg gcctacaaaa acatgcaaat tcaatagatt
    47701 gcagagatta tgtaggcctg ataagcgtag cgcatcaggc aattttgcgt ttgtaatcag
    47761 tctcgagcgg gtcagtttac tggcccgctt tttttatgcg ttattattgc tgaagtgctt
    47821 aatgccaaat catcaatgca atcaggctga ccaccaccag accacacaat gcgctggtca
    47881 gaataaactg ccgacgcacc cgctcacagc ggcgaataaa ttcatcatcg tgatgatcgc
    47941 gataacgttg ggcatagata taccaaacga gacgcacctg tttgttgggt tggccatgtg
    48001 aggtaaaaaa gccccctcca tcaacatatt gatagagcaa tggatcgcag ttacgcagta
    48061 ccactaacaa cgcgcgtagt gatgagaaat agcgcgccat gttaacaatg caaacgacac
    48121 ataaagccca aaataatgcg acggtgctta tcatacctcc tccccggcga cctgcccgcg
    48181 gagttccacc ccggggctac cgctcccgat acgctgccaa tcagttaaca ccaggtcctg
    48241 gagaaaccgc ttttgtggtg accaacatcc gagcggctct atagatagtg taggagatca
    48301 ggttgttttt tttccagaag gttaaccact atcaatatat tcatgtcgaa aatttgttta
    48361 tctaacgggt aagcaaggcg gattgacgga tcatccgggt cgctataagg taaggatggt
    48421 cttaacactg aatctttacg gctgggttag ccccgcgcac gtagttcgca ggacgcgggt
    48481 gacgtaacgg cacaagaaac gctagctggc cagtcatcga caactttaat ggaaggagta
    48541 acactatggc ttataaacac attctcatcg cggtcgatct ctccccggaa agcaaagttc
    48601 tggtagagaa agcagtctct atggctcgcc cctacaatgc gaaagtttct ctgatccacg
    48661 tagatgtaaa ctactctgac ctatacaccg ggcttattga tgtgaatctg ggtgatatgc
    48721 agaaacgcat ctctgaagag acacatcatg cgctgaccga gctttccact aatgcaggct
    48781 acccaatcac tgaaaccctg agcggcagcg gcgacctggg ccaggttctg gtcgatgcaa
    48841 tcaagaaata cgatatggat ctggtggttt gtggtcacca ccaggacttc tggagcaaac
    48901 taatgtcttc cgcacgtcag ctgatcaaca ccgttcacgt tgatatgctg attgttccgc
    48961 tgcgcgacga agaagaataa tcatcccccc acgacgtgtt cctgaacgcc cgcatatgcg
    49021 ggcgttttgc tttttggcgc gccttgttac ctgatgcagc gtaaacccct tatctggcct
    49081 acgatctgcg tacgcaatca aaatctcctg ccaatacaac atttaacact atcatatttt
    49141 ccatcattag tgtgatcagc tggttatttt ctgttgtaat agtgtattaa tctattcacc
    49201 gcatcaatat taagaatctc tgacagatgt aaactttttc gcgcgttatc ccttacgcgt
    49261 tcatactttt caggatggta ttggaaggtt aataaatatg aatacaacaa cacccatggg
    49321 gatgctgcag caacctcgcc catttttcat gatctttttt gtcgagttat gggagcgatt
    49381 cggctactac ggcgtgcagg gcgtactggc ggttttcttc gttaaacagc ttggattctc
    49441 gcaagagcag gcttttgtca cttttggtgc ttttgctgcg ctggtctatg gcctcatttc
    49501 cattggcggc tatgtcggcg accacctgct ggggaccaaa cgcaccattg ttctcggagc
    49561 acttgtgctg gcgattggct acttcatgac cggcatgtcg ctacttaagc ctgacctgat
    49621 tttcatcgcc ctggggacta tcgctgtcgg taacggcctg tttaaagcta acccagccag
    49681 cttgctttcg aagtgctatc cgccgaaaga tccgcggctt gatggcgcat tcaccctgtt
    49741 ctatatgtcg atcaatatcg gctcgttgat agcgttatcg ctggcccctg tgatcgctga
    49801 tagatacggt tattcagtca cctacaacct gtgcggggcg gggttaatta tcgcattact
    49861 ggtttacatc gcctgtcgtg gaatggtgaa agacattggt tctgaacccg acttcaagcc
    49921 gatgagcttc agcaaactgt tgtacgtatt acttggcagc gtggtgatga tcttcgtatg
    49981 tgcatggctg atgcacaacg tagaagtcgc caatctggtg ctgattgttc tctccatcgt
    50041 cgtcaccatc atcttctttc gtcaggcatt caagctggat aaaaccgggc gcaataaaat
    50101 gtttgtcgcc tttgtcctga tgctcgaagc ggtggtgttt tacattctct acgcccagat
    50161 gccaacgtcg ctgaacttct ttgccatcaa caacgtgcat catgaaattc tcggcttttc
    50221 cataaacccg gtcagcttcc aggcgcttaa cccgttctgg gtggtactcg ccagcccaat
    50281 actggcaggc atttacacgc atctgggtaa caaaggcaaa gacctctcga tgccgatgaa
    50341 atttactctc ggcatgttta tgtgctcact gggctttttg acggcggcag ctgcgggaat
    50401 gtggtttgcg gatgcacaag ggctgacatc gccatggttt atcgtgctgg tgtatttatt
    50461 ccagagcctg ggtgaattgt ttattagcgc ccttggcctg gcgatgattg ctgccctggt
    50521 gccgcagcat ttgatgggct ttattctcgg gatgtggttc ctgacgcagg ctgccgcatt
    50581 cttgctgggc ggctatgtgg caacatttac cgcagtgccg gacaacatta ccgatccgct
    50641 tgagacgtta cccgtctata ccaacgtgtt tggtaagatt ggcctggtta cgctgggcgt
    50701 tgcagtagtg atgctgttga tggtgccgtg gctgaaacgc atgattgcga cgccggaaag
    50761 ccattaatta ttcttgcaga aagcagggta gcgttatcgc taccctgctt tacttttaca
    50821 cgggcgtacc tgcatagata tcaaagcgat gccctttagt cacaaccgcg tttggcgtgg
    50881 cgacattcgc tagcggtggc gcgtagtccg ggcgctttac taccacgcgt ttggtcgcca
    50941 gcaagcgtgc aggctccagc aatccatcgg catcaagatc cggccccacc agcgactgaa
    51001 agacacgcat ctcttttttc accagcgcgc ttttctgctt atgcgggaac atcgggtcga
    51061 gatagaccac ctgcgggcgt ggggtaatat cggttagcgc cgtcaggctg gaggcgtgaa
    51121 ttaactgcaa acgctcctgc aaccagccgc cgatttccgc atccgcataa ccacgcgcca
    51181 ggccgtcgtc gagcagcgcg gcaacaactg gattacgctc cagcatccgc acgcggcaac
    51241 cgactgaagc cagcacaaag gcatcgcgcc ccagtcctgc agtggcatcc accacatccg
    51301 gcaaataatc gcctttaatg cccaccgctt tcgccaccgc ctcaccgcga ccaccgccga
    51361 atttgcgtcg gtgcgccatc gctccgccaa caaaatcaac aaagatgccg ccaagttttg
    51421 gctcatcacg cttgcgcaat tccagatgtt ccggcgttaa caccagcgcc atcaggttgt
    51481 cttcatcgtg ctccagcccc cagcgggccg ccagaacaga taaggcgccg tctccggcgc
    51541 ctgtttcatc aattaagcag attttcactg aatgatcagc ccttaatgcc gtaatgctcc
    51601 agcatcgcat ccagctgcgg ttcacgacca cggaagcgtt tgaacagatc catcggctct
    51661 tctgaaccgc cacggctcag aatgttgtcg aggaacgact gcccggtttc acggttgaaa
    51721 atgccctctt cctcaaagcg cgagaaagca tctgccgcca gtacgtcagc ccacaggtag
    51781 ctgtagtaac ctgccgcata accaccggcg aaaatatggc tgaaagcgtg cgggaaacgg
    51841 ccccaggacg gagatggcac cacggcaacc agtttcttga tttctgccag agtttcgagg
    51901 atttttgccc cctgatccgg gcggaactcg gcatgaaggc ggaaatcaaa caggccgaac
    51961 tccagctgac gcagaataaa cagcgccgcc tggtagttct tcgccgccag cattttatcc
    52021 agcaactctt tcggcagcgg ttcgccggtt tcatagtgac cagagataaa cgccagcgcc
    52081 tccggctccc agcaccagtt ttccataaac tgactcggca gttcgaccgc atcccacggc
    52141 acaccgctga taccggaaac accagcggtt tcgatgcggg tcagcatatg gtgcaggccg
    52201 tgaccgaact cgtggaacag ggtgatcact tcgtcgtgag tgaacagcgc cggtttacca
    52261 tttaccgggc ggttgaagtt acaagtcaaa tacgcgaccg gtttttgcag agaaccatca
    52321 gctttacgca tctggcctac gcagtcatcc atccacgccc cgccgcgctt gttttcacgg
    52381 gcatacagat cgaggtagaa gctgccgcgc agttcgttat tttcgtcata cagttcgaag
    52441 aaacgtacat ccggatgcca gacatcaacc tctttacgct ctttagcggt gatgccgtaa
    52501 atacgtttca ccacttcaaa caggccgtta accgctttgt tttccgggaa gtacggacgc
    52561 agctgctcgt cgctgatgct gtagaggtgc tgtttctgtt tttcgctgta gtaagcgata
    52621 tcccacggct gcaactcatc gacaccaaat tcggctttag cgaaggcacg cagttgctcc
    52681 agctcttttt cgccttgcgg acgcgcgcgt tttgccagat cggttaagaa atccagcacc
    52741 tgctgcgggt tttctgccat tttagtggca agggatttaa aggcgtagtt ttcaaagccc
    52801 agcagttgcg ccagttcgtg acgcagcgcg aggatctctt ccatcacctt gctgttatcc
    52861 cacttaccgg cgttcgggcc ttgatcggag gcgcgggtgc tgtaagcgcg atacatctct
    52921 tcgcgcagag cctggttgtc gcagtaggtc attaccggca ggtagcttgg gatatccagc
    52981 gtcagcaggt agccttccag ctctttcgct tcggcctggg cttttgccgc agccagcgcg
    53041 ctttctggca tccccgccag ccccgcttcg tcggtaacga gtttggtcca gcccatcgtc
    53101 gcatcgagga cgttgttgct gtactggttg cccagttcag aaagacgggt agcaatttcg
    53161 ccgtaacgct gctgtttctc tttcggcaga cctatgccag agagttcaaa atcacgcagg
    53221 gcgttatcaa ccgctttctt ctgcgccgtg ttcagcgtgg cgtaatgatc gccatcgcgc
    53281 aggtcgcggt acgctttata cagcccttca tgttgcccta cccaggtgct gtattccgac
    53341 agcagcggca gggtttgctc gtaggcttca cgcagttccg ggctattttt caccgagttc
    53401 aggtggctga ccggggagaa gatacgcccc aacacatcgt ccacttccgc caacggctgg
    53461 cagagatttt cccaggtgta cggtgcccct tgcgctacta cgcgcgccac attttcgcgg
    53521 caatcgttca gcgcctgagt cacggctgga acgacatgtt ccgggagaat tttagaaaac
    53581 ggaggcaatt caaagggagt cagtaacgga ttcgtcattc gcgcagtcct ggttaaagag
    53641 gttaaggaag cgctcaacag gcgctttaca taatgtgtgt agcatggggt taagtgtagt
    53701 gaatttcaat gagaaacgtt acgctttcgc ggcggcggcc tcttttcggt atactgtcct
    53761 gatacgcttt tgtgcgcccc gaatacgggc cgatttttac ttaccggaac acctttaccc
    53821 atgctcagtt atcgccacag ctttcacgct ggcaaccacg ccgacgtcct taaacatacc
    53881 gttcagagcc tgatcatcga gtcgctgaaa gagaaagata aaccgtttct ctatctcgat
    53941 acccacgcag gggccgggcg ttatcagtta ggcagcgaac atgccgagcg taccggcgaa
    54001 tatctcgaag gtatcgcccg tatctggcag caggacgatt tgcccgcaga actggaggcg
    54061 tacatcaatg tggtaaaaca cttcaaccgt agcggtcagt tgcgttacta ccccgggtcg
    54121 ccgttgattg ctcgccagct actgcgcgaa caagacagcc tgcaactgac cgaactgcac
    54181 ccgagcgatt acccgttgct gcgttctgaa tttcagaaag acagccgtgc gcgcgtcgaa
    54241 aaagccgacg gtttccagca gcttaaggcc aaacttccgc cggtttcccg ccgtggttta
    54301 atccttatcg acccgccgta tgaaatgaaa accgactatc aggcggtggt cagcgggata
    54361 gcagaaggtt acaaacgttt cgccactggt acttacgcac tgtggtatcc ggtggtgctg
    54421 cgtcagcaaa ttaagcgcat gatccacgat ctggaagcga ccggtattcg caaaattctg
    54481 caaattgaac tggcggtact gccagacagc gatcgccgtg gcatgaccgc ttccggcatg
    54541 attgtgatta acccgccgtg gaaactggaa cagcagatga ataacgtgct gccgtggctg
    54601 cacagcaaac tggttccggc aggcaccggg cacgccaccg taagctggat cgtgccggag
    54661 taattgcagc cattgctggc acctattacg tctcgcgcta caatcgcggt aatcaacgat
    54721 aaggacactc tgtcatgact aaacactatg attacatcgc catcggcggc ggcagcggcg
    54781 gtatcgcctc catcaaccgc gcggctatgt acggccagaa atgcgcactg attgaagcca
    54841 aagagctggg cggcacctgc gtgaacgtcg gctgcgtgcc gaaaaaagtg atgtggcacg
    54901 cggcgcaaat ccgtgaagcg atccatatgt acggccctga ttatggtttc gataccacca
    54961 tcaacaagtt caactgggaa acgttgatcg ccagccgtac cgcctatatc gaccgtattc
    55021 atacttccta tgaaaacgtg ctcggtaaaa ataacgttga tgtaatcaaa ggctttgccc
    55081 gcttcgttga tgccaaaacg ctggaggtaa acggcgaaac catcacggcc gatcatattc
    55141 tgatcgccac aggcggtcgt ccgagccacc cggatattcc gggcgtggaa tacggtattg
    55201 attctgatgg cttcttcgcc cttcctgctt tgccagagcg cgtggcggtt gttggcgcgg
    55261 gttacatcgc cgttgagctg gcgggcgtga ttaacggcct cggcgcgaaa acgcatctgt
    55321 ttgtgcgtaa acatgcgccg ctgcgcagct tcgacccgat gatttccgaa acgctggtcg
    55381 aagtgatgaa cgccgaaggc ccgcagctgc acaccaacgc catcccgaaa gcggtagtga
    55441 aaaatgccga tggtagcctg acgctggagc tggaagatgg tcgcagtgaa acggtggatt
    55501 gcctgatttg ggcgattggt cgcgagcctg ccaatgacaa catcaaccta gaagccgctg
    55561 gcgttaaaac taacgaaaaa ggctatatcg tcgtcgataa atatcaaaac accaatattg
    55621 aaggtattta cgcggtgggc gataacacgg gtgcagtgga actgacaccg gtagcagttg
    55681 cagcgggtcg ccgtctctct gaacgcctgt ttaataacaa gccggatgag catctggatt
    55741 acagcaacat tccgaccgtg gtcttcagcc atccgccgat tggtactgtt ggtttaacgg
    55801 aaccgcaggc gcgcgagcag tacggcgacg atcaggtgaa agtgtataaa tcctctttca
    55861 ccgcgatgta taccgccgtc accactcacc gccagccgtg ccgcatgaag ctggtgtgcg
    55921 ttgggccaga agagaagatt gtcggtattc acggcattgg ctttggtatg gacgaaatgt
    55981 tgcagggctt cgcggtggcg ctgaagatgg gcgcaaccaa aaaagacttc gacaataccg
    56041 tcgccattca cccaacggcg gcagaagagt tcgtgaccat gcgctaaatg ttaaagggct
    56101 aagagtagtg tgctcttagc ccttaattac gtttccgcta tcagttcaga agctgaagca
    56161 gaaagcggat cagttccagc agcgcaatta acgctccaag aacgatgatt gctttatcaa
    56221 tcacccgttt tctccatgcg atggagtgag aatgcatccg cttactcatc cactgcctgt
    56281 cacggcgcat gtctcattgt tagatgacga ctatctcact ccggccagag catcagttaa
    56341 cggcaccacc cgtacttctg accaggactt tgaaagcgtt tatgcgcatt accagagtga
    56401 aaatgcctca gagctaactg gataatcata cagtacattc aggttataaa accagcatgt
    56461 ccttgcaata gtttcagtat ggtattagca ttgatgcgtt agatgatgac tatctcactc
    56521 cagccagagc caccaactca gggctgaaga gtaaaaaaac cgacgcaaag tcggtttttt
    56581 tacatccgga ttcggacaag gcttaatatg acgatgaccc agtgaaagta tataaatcgt
    56641 cattgcgata tataccgaag tgccccctcc gccagctgaa gaaatcgcta attcttgcaa
    56701 tgttagccac tggctaatag tattgaaccg ttagatgtgg gcattctcac tccagccaga
    56761 gccaccaact caggctgaag agtaaaaaac cgacgcaaag tcggtttttt tacgtcctga
    56821 ttcaaaccgc ctttcaaatg aatagccaac tcaaaattca cacctattac cttcctctgc
    56881 acttacacat tcgttaagtc atatatgttt ttgacttatc cgcttcgaag agagacacta
    56941 cctgccacaa tcaggagcgc aatatgtcat ttctgttacc catccaattg ttcaaaattc
    57001 ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgagctggga gagttatgcg
    57061 tctgcgatct ttgtactgcc ctcgatcagt cgcagcccaa gatctcccgc cacctggcat
    57121 tgcttcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt cattaccgct
    57181 tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg cgatgtgaac
    57241 aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc ggggacagta
    57301 agaacatttg cagttaaaaa tttagctaaa cacatatgaa ttttcagatg tgttttatcc
    57361 gggaggcatt atgttactgg caggcgctat ctttgtcctg accatcgtat tggttatctg
    57421 gcagccgaaa ggtttaggca tcggctggag tgcaacgctc ggcgcagtac tggcgttagt
    57481 tacgggcgtg gttcatccgg gtgatattcc ggtggtgtgg aatatcgtct ggaacgcgac
    57541 ggctgcgttt atcgccgtca ttatcatcag cctgctgctg gatgagtccg gcttttttga
    57601 atgggcggcg ctgcacgtct cacgctgggg taatggtcgt ggtcgcttgc tgtttacctg
    57661 gattgtcctg ctcggtgctg ccgttgccgc cctgtttgcc aatgatggcg cggcgcttat
    57721 tttgacgccg attgtcatcg ccatgctgct ggctttagga ttcagtaaag gcacgacgct
    57781 ggcgttcgtg atggcggccg gattcattgc cgataccgcc agcctgccgc ttattgtctc
    57841 caacctggtg aatatcgttt ccgctgattt ctttggcctc ggctttcgcg aatacgcctc
    57901 ggtgatggtg ccggtggata tcgccgcgat tgttgccacg ctggtgatgc tacatctcta
    57961 ttttcgcaaa gatattccgc agaactacga tatggcgctg ctgaaatctc ccgcagaagc
    58021 gatcaaagat cctgctacgt tcaaaactgg ctgggttgtt ttactgcttc tgctggtggg
    58081 atttttcgtc ctggaaccgc tcggcattcc ggtgagcgcc attgcagctg tgggcgcgct
    58141 gatattattt gtcgtcgcta aacgcggtca tgcgattaat acgggtaaag tcctgcgcgg
    58201 tgccccctgg cagattgtca tcttctcgct cggcatgtat ctggtggttt atggcctgcg
    58261 caatgccgga ttaacggaat atctttctgg cgtactcaac gtgctggcgg ataacggcct
    58321 gtgggccgcg acgctcggca ccggattcct caccgccttc ctctcttcta ttatgaacaa
    58381 tatgccgacg gtactggttg gcgcgttgtc cattgatggc agcacggcat ctggcgttat
    58441 caaagaagcg atggtttatg ccaatgtgat tggctgcgat ttgggaccga aaattacccc
    58501 aattggtagc ctggctacgc tactctggct gcacgtactt tcgcagaaga atatgactat
    58561 cagctgggga tattacttcc gtacagggat tatcatgacc ctgcctgtgc tgtttgtgac
    58621 gctggctgcg ctggcgctac gtctctcttt cactttgtaa tgagatactg atatgagcaa
    58681 cattaccatt tatcacaacc cggcctgcgg cacgtcgcgt aatacgctgg agatgatccg
    58741 caacagcggc acagaaccga ccattatcca ttatctggaa actccgccaa cgcgcgatga
    58801 actggtcaaa ctcattgccg atatggggat ttccgtacgc gcgctgctgc gtaaaaacgt
    58861 cgaaccgtat gaggagctgg gccttgcgga agataaattt actgacgatc ggttaatcga
    58921 ctttatgctt cagcacccga ttctgattaa tcgcccgatt gtggtgacgc cgctgggaac
    58981 tcgcctgtgc cgcccttcag aagtggtgct ggaaattctg ccagatgcgc aaaaaggcgc
    59041 attctccaag gaagatggcg agaaagtggt tgatgaagcg ggtaagcgcc tgaaataaag
    59101 cggcgatatc cactgccgat agttggtaaa gtgcgctttg tttatgtcgg atgcgacgct
    59161 aacgcgtctt atccgaccta caaaaccgtg caaattcaat atatggcatg aaatatgtag
    59221 agcgtagcgc atcaggcaat tttgctattt acgcagcact cctcgcgtaa gaattcaatt
    59281 atcgcttcca gcattgcgta ttgcgaaaca cacatcagcg tccgactctc acggttttgt
    59341 ttgaccagtc ctaccgatac cagtgctgat atatggtggc tgagtgtgga gccaggtatc
    59401 ccaagctgct tttgcaactc accgaccggc aacccttgtt ccccggcttt gacaagatgt
    59461 ttaaatatga acaaacgggt cgggtgacct agctctttca gcgctttagc gacttcttcc
    59521 aactgcatat gacacccctc tgtctggttg atatttcgat aataccagaa atatcttgat
    59581 ctgaatttca tttcgattat tctggaaata tagatttatc caatgaggtt acgtatgagt
    59641 agttggcttg cgatgctgca agatgccgca gagatgtttg tgtttctcgc cgtcgagctt
    59701 tcgttgctgt ttatagtgat tagtgccggt gtcagcctga taagacaaaa ggtgccagac
    59761 cataaaatcc agcagatgat gggggccaga aaagggagag gctatctcct ggctgctctg
    59821 ttgggagccg ttaccccgtt ctgtagttgc tcgacaatac ccatgttacg tggattgttg
    59881 tcagcgaaag ccgggt
//
LOCUS       DAZRVY010000010        58386 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZRVY010000010 DAZRVY010000000
VERSION     DAZRVY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.010"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHXOX_000617"
     CDS             <1..161
                     /locus_tag="ACHXOX_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR7004188.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHXOX_000618"
     CDS             complement(199..1260)
                     /locus_tag="ACHXOX_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR7004189.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHXOX_000619"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHXOX_000619"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR7004190.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHXOX_000620"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHXOX_000620"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR7004191.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHXOX_000621"
     CDS             complement(3454..4155)
                     /locus_tag="ACHXOX_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR7004192.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHXOX_000622"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHXOX_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR7004193.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHXOX_000623"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHXOX_000623"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR7004194.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHXOX_000624"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHXOX_000624"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR7004195.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHXOX_000625"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHXOX_000625"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR7004196.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHXOX_000626"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHXOX_000626"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR7004197.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHXOX_000627"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHXOX_000627"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR7004198.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHXOX_000628"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHXOX_000628"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR7004199.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="ACHXOX_000629"
     CDS             complement(14498..14650)
                     /locus_tag="ACHXOX_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004200.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="ACHXOX_000630"
     CDS             14668..14859
                     /locus_tag="ACHXOX_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR7004201.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15170..15688
                     /locus_tag="ACHXOX_000631"
     CDS             15170..15688
                     /locus_tag="ACHXOX_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR7004202.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15704..16243
                     /locus_tag="ACHXOX_000632"
     CDS             15704..16243
                     /locus_tag="ACHXOX_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR7004203.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="ACHXOX_000633"
     CDS             complement(16338..17915)
                     /gene="guaA"
                     /locus_tag="ACHXOX_000633"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR7004204.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="ACHXOX_000634"
     CDS             complement(17984..19450)
                     /gene="guaB"
                     /locus_tag="ACHXOX_000634"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR7004205.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19612..20982
                     /gene="xseA"
                     /locus_tag="ACHXOX_000635"
     CDS             19612..20982
                     /gene="xseA"
                     /locus_tag="ACHXOX_000635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR7004206.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(20979..21194)
                     /locus_tag="ACHXOX_000636"
     CDS             complement(20979..21194)
                     /locus_tag="ACHXOX_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR7004207.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21263..22735)
                     /gene="der"
                     /locus_tag="ACHXOX_000637"
     CDS             complement(21263..22735)
                     /gene="der"
                     /locus_tag="ACHXOX_000637"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR7004208.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="ACHXOX_000638"
     CDS             complement(22853..24031)
                     /gene="bamB"
                     /locus_tag="ACHXOX_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR7004209.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="ACHXOX_000639"
     CDS             complement(24042..24662)
                     /gene="yfgM"
                     /locus_tag="ACHXOX_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR7004210.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="ACHXOX_000640"
     CDS             complement(24680..25954)
                     /gene="hisS"
                     /locus_tag="ACHXOX_000640"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR7004211.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="ACHXOX_000641"
                     /gene_synonym="gcpE"
     CDS             complement(26065..27183)
                     /gene="ispG"
                     /locus_tag="ACHXOX_000641"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR7004212.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="ACHXOX_000642"
     CDS             complement(27210..28223)
                     /gene="rodZ"
                     /locus_tag="ACHXOX_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR7004213.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28508..29662)
                     /locus_tag="ACHXOX_000643"
     CDS             complement(28508..29662)
                     /locus_tag="ACHXOX_000643"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR7004214.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="ACHXOX_000644"
     CDS             complement(29812..30243)
                     /gene="ndk"
                     /locus_tag="ACHXOX_000644"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR7004215.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="ACHXOX_000645"
     CDS             complement(30392..32704)
                     /gene="pbpC"
                     /locus_tag="ACHXOX_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR7004216.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="ACHXOX_000646"
     CDS             complement(32705..37666)
                     /gene="yfhM"
                     /locus_tag="ACHXOX_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR7004217.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37873..38718
                     /gene="sseA"
                     /locus_tag="ACHXOX_000647"
     CDS             37873..38718
                     /gene="sseA"
                     /locus_tag="ACHXOX_000647"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR7004218.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            39054..39179
                     /gene="timP"
                     /locus_tag="ACHXOX_000648"
     CDS             39054..39179
                     /gene="timP"
                     /locus_tag="ACHXOX_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR7004219.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="ACHXOX_000649"
     CDS             complement(39211..39987)
                     /gene="sseB"
                     /locus_tag="ACHXOX_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR7004220.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="ACHXOX_000650"
     CDS             complement(40129..41412)
                     /gene="pepB"
                     /locus_tag="ACHXOX_000650"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR7004221.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="ACHXOX_000651"
     CDS             complement(41590..41790)
                     /gene="iscX"
                     /locus_tag="ACHXOX_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR7004222.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="ACHXOX_000652"
     CDS             complement(41802..42137)
                     /gene="fdx"
                     /locus_tag="ACHXOX_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR7004223.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="ACHXOX_000653"
     CDS             complement(42139..43989)
                     /gene="hscA"
                     /locus_tag="ACHXOX_000653"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR7004224.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="ACHXOX_000654"
     CDS             complement(44006..44521)
                     /gene="hscB"
                     /locus_tag="ACHXOX_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR7004225.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="ACHXOX_000655"
     CDS             complement(44617..44940)
                     /gene="iscA"
                     /locus_tag="ACHXOX_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR7004226.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="ACHXOX_000656"
     CDS             complement(44957..45343)
                     /gene="iscU"
                     /locus_tag="ACHXOX_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR7004227.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="ACHXOX_000657"
     CDS             complement(45371..46585)
                     /gene="iscS"
                     /locus_tag="ACHXOX_000657"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR7004228.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="ACHXOX_000658"
     CDS             complement(46826..47314)
                     /gene="iscR"
                     /locus_tag="ACHXOX_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR7004229.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="ACHXOX_000659"
     CDS             complement(47615..48352)
                     /gene="trmJ"
                     /locus_tag="ACHXOX_000659"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR7004230.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48471..49274
                     /gene="suhB"
                     /locus_tag="ACHXOX_000660"
     CDS             48471..49274
                     /gene="suhB"
                     /locus_tag="ACHXOX_000660"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR7004231.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49419..50273
                     /locus_tag="ACHXOX_000661"
     CDS             49419..50273
                     /locus_tag="ACHXOX_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7004232.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50464..51744
                     /gene="csiE"
                     /locus_tag="ACHXOX_000662"
     CDS             50464..51744
                     /gene="csiE"
                     /locus_tag="ACHXOX_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR7004233.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51736..52875)
                     /locus_tag="ACHXOX_000663"
     CDS             complement(51736..52875)
                     /locus_tag="ACHXOX_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR7004234.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="ACHXOX_000664"
     CDS             complement(53035..53925)
                     /gene="hcaR"
                     /locus_tag="ACHXOX_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR7004235.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            54061..55422
                     /gene="hcaE"
                     /locus_tag="ACHXOX_000665"
     CDS             54061..55422
                     /gene="hcaE"
                     /locus_tag="ACHXOX_000665"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR7004236.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55419..55937
                     /gene="hcaF"
                     /locus_tag="ACHXOX_000666"
     CDS             55419..55937
                     /gene="hcaF"
                     /locus_tag="ACHXOX_000666"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR7004237.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            55937..56257
                     /locus_tag="ACHXOX_000667"
     CDS             55937..56257
                     /locus_tag="ACHXOX_000667"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR7004238.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56254..57066
                     /gene="hcaB"
                     /locus_tag="ACHXOX_000668"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56254..57066
                     /gene="hcaB"
                     /locus_tag="ACHXOX_000668"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR7004239.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57076..58278
                     /gene="hcaD"
                     /locus_tag="ACHXOX_000669"
     CDS             57076..58278
                     /gene="hcaD"
                     /locus_tag="ACHXOX_000669"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR7004240.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14270 a        15786 c        14569 g        13761 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaatt ttaataaatt taatctatcc ctttatacgc aatacattta ctttcctctt
    15121 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
    15181 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
    15241 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
    15301 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
    15361 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
    15421 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
    15481 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
    15541 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
    15601 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
    15661 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
    15721 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
    15781 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
    15841 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
    15901 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
    15961 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
    16021 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
    16081 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
    16141 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
    16201 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
    16261 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
    16321 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
    16381 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
    16441 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
    16501 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
    16561 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
    16621 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
    16681 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
    16741 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
    16801 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
    16861 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
    16921 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
    16981 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
    17041 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
    17101 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
    17161 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
    17221 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
    17281 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
    17341 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
    17401 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
    17461 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
    17521 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
    17581 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
    17641 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
    17701 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
    17761 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
    17821 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga
    17881 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
    17941 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
    18001 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
    18061 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
    18121 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
    18181 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
    18241 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
    18301 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
    18361 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
    18421 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
    18481 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
    18541 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
    18601 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
    18661 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
    18721 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
    18781 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
    18841 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
    18901 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
    18961 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
    19021 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
    19081 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
    19141 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
    19201 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
    19261 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
    19321 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
    19381 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
    19441 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
    19501 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
    19561 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
    19621 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
    19681 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
    19741 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
    19801 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
    19861 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
    19921 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
    19981 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
    20041 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
    20101 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
    20161 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
    20221 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
    20281 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
    20341 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
    20401 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
    20461 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
    20521 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
    20581 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
    20641 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
    20701 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
    20761 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
    20821 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
    20881 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
    20941 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
    21001 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
    21061 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
    21121 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
    21181 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
    21241 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
    21301 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
    21361 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
    21421 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
    21481 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
    21541 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
    21601 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
    21661 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
    21721 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
    21781 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
    21841 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca atggcctgca
    21901 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
    21961 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
    22021 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
    22081 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
    22141 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
    22201 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
    22261 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
    22321 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
    22381 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
    22441 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
    22501 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
    22561 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
    22621 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
    22681 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
    22741 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
    22801 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
    22861 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
    22921 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
    22981 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
    23041 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
    23101 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
    23161 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
    23221 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
    23281 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
    23341 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
    23401 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
    23461 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
    23521 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
    23581 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
    23641 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
    23701 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
    23761 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
    23821 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
    23881 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
    23941 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
    24001 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
    24061 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
    24121 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
    24181 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
    24241 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
    24301 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
    24361 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
    24421 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
    24481 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
    24541 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
    24601 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
    24661 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
    24721 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
    24781 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
    24841 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
    24901 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
    24961 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
    25021 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
    25081 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    25141 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    25201 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    25261 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    25321 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    25381 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    25441 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    25501 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    25561 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    25621 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    25681 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    25741 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    25801 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    25861 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    25921 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    25981 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    26041 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    26101 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    26161 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    26221 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    26281 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    26341 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    26401 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    26461 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    26521 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    26581 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    26641 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    26701 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    26761 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    26821 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    26881 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    26941 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    27001 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    27061 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    27121 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    27181 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    27241 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    27301 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    27361 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    27421 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    27481 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    27541 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    27601 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    27661 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    27721 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    27781 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    27841 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    27901 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    27961 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    28021 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    28081 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    28141 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    28201 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    28261 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    28321 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    28381 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    28441 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    28501 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    28561 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatca atatcatcac
    28621 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    28681 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    28741 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    28801 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    28861 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    28921 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    28981 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    29041 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    29101 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    29161 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    29221 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    29281 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    29341 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    29401 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    29461 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    29521 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    29581 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    29641 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    29701 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    29761 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    29821 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    29881 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    29941 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    30001 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    30061 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    30121 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    30181 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    30241 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    30301 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    30361 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    30421 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    30481 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    30541 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    30601 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    30661 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    30721 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    30781 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    30841 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    30901 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    30961 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    31021 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    31081 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    31141 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    31201 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    31261 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    31321 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    31381 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    31441 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    31501 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    31561 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    31621 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    31681 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    31741 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    31801 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    31861 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    31921 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    31981 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    32041 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    32101 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    32161 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    32221 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    32281 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    32341 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    32401 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    32461 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    32521 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    32581 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    32641 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    32701 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    32761 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    32821 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    32881 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    32941 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    33001 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    33061 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    33121 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    33181 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    33241 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    33301 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    33361 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    33421 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    33481 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    33541 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    33601 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    33661 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    33721 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    33781 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    33841 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    33901 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    33961 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    34021 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    34081 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    34141 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    34201 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    34261 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    34321 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    34381 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    34441 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    34501 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    34561 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    34621 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    34681 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    34741 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    34801 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    34861 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    34921 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    34981 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    35041 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    35101 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    35161 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    35221 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    35281 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    35341 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    35401 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    35461 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    35521 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    35581 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    35641 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    35701 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    35761 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    35821 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    35881 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    35941 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    36001 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    36061 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    36121 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    36181 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    36241 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    36301 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    36361 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    36421 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    36481 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    36541 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    36601 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    36661 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    36721 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    36781 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    36841 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    36901 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    36961 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    37021 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    37081 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    37141 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    37201 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    37261 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    37321 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    37381 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    37441 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    37501 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    37561 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    37621 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    37681 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    37741 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    37801 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    37861 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    37921 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    37981 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    38041 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    38101 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    38161 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    38221 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    38281 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    38341 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    38401 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    38461 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    38521 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    38581 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    38641 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    38701 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    38761 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    38821 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    38881 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    38941 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    39001 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    39061 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    39121 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    39181 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    39241 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    39301 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    39361 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    39421 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    39481 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    39541 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    39601 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    39661 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    39721 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    39781 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    39841 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    39901 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    39961 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    40021 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    40081 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    40141 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    40201 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    40261 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    40321 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    40381 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    40441 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    40501 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    40561 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    40621 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    40681 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    40741 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    40801 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    40861 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    40921 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    40981 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    41041 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    41101 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    41161 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    41221 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    41281 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    41341 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    41401 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    41461 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    41521 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    41581 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    41641 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    41701 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    41761 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    41821 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacgcg tgcctggcag
    41881 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    41941 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    42001 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    42061 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    42121 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    42181 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    42241 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    42301 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    42361 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    42421 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    42481 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    42541 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    42601 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    42661 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    42721 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    42781 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    42841 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    42901 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    42961 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    43021 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    43081 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    43141 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    43201 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    43261 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    43321 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    43381 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    43441 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    43501 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    43561 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    43621 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    43681 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    43741 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    43801 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    43861 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    43921 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    43981 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    44041 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    44101 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    44161 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    44221 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    44281 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    44341 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    44401 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    44461 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    44521 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    44581 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    44641 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    44701 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    44761 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    44821 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    44881 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    44941 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    45001 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    45061 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    45121 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    45181 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    45241 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    45301 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    45361 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    45421 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    45481 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    45541 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    45601 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    45661 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    45721 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    45781 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    45841 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    45901 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    45961 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    46021 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    46081 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    46141 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    46201 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    46261 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    46321 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    46381 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    46441 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    46501 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    46561 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    46621 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    46681 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    46741 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    46801 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    46861 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    46921 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    46981 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    47041 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    47101 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    47161 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    47221 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    47281 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    47341 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    47401 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    47461 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    47521 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    47581 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    47641 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    47701 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    47761 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    47821 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    47881 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    47941 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    48001 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    48061 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    48121 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    48181 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    48241 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    48301 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    48361 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    48421 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    48481 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    48541 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    48601 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    48661 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    48721 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    48781 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    48841 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    48901 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    48961 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    49021 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    49081 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    49141 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    49201 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    49261 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    49321 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    49381 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    49441 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    49501 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    49561 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    49621 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    49681 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    49741 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    49801 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    49861 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    49921 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    49981 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    50041 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    50101 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    50161 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    50221 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    50281 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    50341 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    50401 ttcgccatct ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    50461 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    50521 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    50581 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    50641 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    50701 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    50761 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    50821 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    50881 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    50941 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    51001 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    51061 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    51121 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    51181 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    51241 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    51301 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    51361 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    51421 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    51481 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    51541 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    51601 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    51661 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    51721 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    51781 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    51841 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    51901 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    51961 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    52021 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    52081 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    52141 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    52201 gctaaaaccg taataggccg aatgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    52261 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    52321 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    52381 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    52441 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    52501 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    52561 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    52621 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    52681 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    52741 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    52801 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    52861 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    52921 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    52981 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    53041 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    53101 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    53161 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    53221 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    53281 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    53341 cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg
    53401 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    53461 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    53521 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    53581 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    53641 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    53701 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    53761 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    53821 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    53881 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    53941 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc
    54001 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    54061 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    54121 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    54181 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    54241 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    54301 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    54361 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    54421 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    54481 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    54541 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    54601 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    54661 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    54721 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    54781 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    54841 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    54901 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    54961 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    55021 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    55081 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    55141 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    55201 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    55261 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    55321 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    55381 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    55441 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    55501 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    55561 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    55621 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    55681 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    55741 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    55801 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    55861 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    55921 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    55981 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    56041 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    56101 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    56161 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    56221 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    56281 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    56341 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    56401 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    56461 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    56521 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    56581 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    56641 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    56701 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    56761 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    56821 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    56881 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    56941 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    57001 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    57061 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    57121 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    57181 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    57241 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    57301 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    57361 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    57421 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    57481 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    57541 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    57601 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    57661 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    57721 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    57781 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    57841 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    57901 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    57961 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg
    58021 ccgccgtggt tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt
    58081 ggcgatgact ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg
    58141 caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt
    58201 cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc
    58261 gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga
    58321 ggtgcaagag tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac
    58381 acacta
//
LOCUS       DAZRVY010000011        57431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZRVY010000011 DAZRVY010000000
VERSION     DAZRVY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.011"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="ACHXOX_000670"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="ACHXOX_000670"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR7004241.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3124)
                     /locus_tag="ACHXOX_000671"
                     /pseudo
     CDS             complement(1498..3124)
                     /locus_tag="ACHXOX_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3583..3669)
                     /locus_tag="ACHXOX_000672"
     tRNA            complement(3583..3669)
                     /locus_tag="ACHXOX_000672"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:Leu,seq:gag)
     gene            complement(3684..4016)
                     /gene="secG"
                     /locus_tag="ACHXOX_000673"
     CDS             complement(3684..4016)
                     /gene="secG"
                     /locus_tag="ACHXOX_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR7004242.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="ACHXOX_000674"
     CDS             complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="ACHXOX_000674"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR7004243.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5574..6422)
                     /gene="folP"
                     /locus_tag="ACHXOX_000675"
     CDS             complement(5574..6422)
                     /gene="folP"
                     /locus_tag="ACHXOX_000675"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR7004244.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="ACHXOX_000676"
     CDS             complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="ACHXOX_000676"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR7004245.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="ACHXOX_000677"
     CDS             complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="ACHXOX_000677"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR7004246.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9301..9594
                     /gene="yhbY"
                     /locus_tag="ACHXOX_000678"
     CDS             9301..9594
                     /gene="yhbY"
                     /locus_tag="ACHXOX_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR7004247.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9750..10226)
                     /gene="greA"
                     /locus_tag="ACHXOX_000679"
     CDS             complement(9750..10226)
                     /gene="greA"
                     /locus_tag="ACHXOX_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR7004248.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10474..11907
                     /gene="dacB"
                     /locus_tag="ACHXOX_000680"
     CDS             10474..11907
                     /gene="dacB"
                     /locus_tag="ACHXOX_000680"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR7004249.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11962..12069
                     /locus_tag="ACHXOX_000681"
                     /pseudo
     CDS             11962..12069
                     /locus_tag="ACHXOX_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="ACHXOX_000682"
     CDS             complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="ACHXOX_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR7004250.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13281..14246)
                     /locus_tag="ACHXOX_000683"
     CDS             complement(13281..14246)
                     /locus_tag="ACHXOX_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7004251.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="ACHXOX_000684"
     CDS             complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="ACHXOX_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR7004252.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="ACHXOX_000685"
     CDS             complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="ACHXOX_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR7004253.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15221..16192
                     /gene="ispB"
                     /locus_tag="ACHXOX_000686"
     CDS             15221..16192
                     /gene="ispB"
                     /locus_tag="ACHXOX_000686"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR7004254.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16420..16698
                     /gene="sfsB"
                     /locus_tag="ACHXOX_000687"
     CDS             16420..16698
                     /gene="sfsB"
                     /locus_tag="ACHXOX_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR7004255.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16746..18005)
                     /gene="murA"
                     /locus_tag="ACHXOX_000688"
     CDS             complement(16746..18005)
                     /gene="murA"
                     /locus_tag="ACHXOX_000688"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR7004256.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="ACHXOX_000689"
     CDS             complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="ACHXOX_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR7004257.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="ACHXOX_000690"
     CDS             complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="ACHXOX_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR7004258.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="ACHXOX_000691"
     CDS             complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="ACHXOX_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR7004259.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="ACHXOX_000692"
     CDS             complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="ACHXOX_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR7004260.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="ACHXOX_000693"
     CDS             complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="ACHXOX_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR7004261.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="ACHXOX_000694"
     CDS             complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="ACHXOX_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR7004262.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21786..22763
                     /locus_tag="ACHXOX_000695"
     CDS             21786..22763
                     /locus_tag="ACHXOX_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR7004263.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22777..23763
                     /gene="kdsD"
                     /locus_tag="ACHXOX_000696"
     CDS             22777..23763
                     /gene="kdsD"
                     /locus_tag="ACHXOX_000696"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR7004264.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23784..24350
                     /gene="kdsC"
                     /locus_tag="ACHXOX_000697"
     CDS             23784..24350
                     /gene="kdsC"
                     /locus_tag="ACHXOX_000697"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR7004265.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24347..24922
                     /gene="lptC"
                     /locus_tag="ACHXOX_000698"
     CDS             24347..24922
                     /gene="lptC"
                     /locus_tag="ACHXOX_000698"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR7004266.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24891..25448
                     /gene="lptA"
                     /locus_tag="ACHXOX_000699"
     CDS             24891..25448
                     /gene="lptA"
                     /locus_tag="ACHXOX_000699"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR7004267.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25455..26180
                     /gene="lptB"
                     /locus_tag="ACHXOX_000700"
     CDS             25455..26180
                     /gene="lptB"
                     /locus_tag="ACHXOX_000700"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR7004268.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26228..27661
                     /gene="rpoN"
                     /locus_tag="ACHXOX_000701"
     CDS             26228..27661
                     /gene="rpoN"
                     /locus_tag="ACHXOX_000701"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR7004269.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27684..27971
                     /gene="hpf"
                     /locus_tag="ACHXOX_000702"
     CDS             27684..27971
                     /gene="hpf"
                     /locus_tag="ACHXOX_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR7004270.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28089..28580
                     /gene="ptsN"
                     /locus_tag="ACHXOX_000703"
     CDS             28089..28580
                     /gene="ptsN"
                     /locus_tag="ACHXOX_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR7004271.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28626..29480
                     /gene="rapZ"
                     /locus_tag="ACHXOX_000704"
     CDS             28626..29480
                     /gene="rapZ"
                     /locus_tag="ACHXOX_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR7004272.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29477..29749
                     /gene="npr"
                     /locus_tag="ACHXOX_000705"
     CDS             29477..29749
                     /gene="npr"
                     /locus_tag="ACHXOX_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR7004273.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29963..30595
                     /gene="yrbL"
                     /locus_tag="ACHXOX_000706"
     CDS             29963..30595
                     /gene="yrbL"
                     /locus_tag="ACHXOX_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR7004274.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="ACHXOX_000707"
     CDS             complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="ACHXOX_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR7004275.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="ACHXOX_000708"
     CDS             complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="ACHXOX_000708"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR7004276.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="ACHXOX_000709"
     CDS             complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="ACHXOX_000709"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR7004277.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34632..35561)
                     /locus_tag="ACHXOX_000710"
     CDS             complement(34632..35561)
                     /locus_tag="ACHXOX_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR7004278.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36236..40696
                     /gene="gltB"
                     /locus_tag="ACHXOX_000711"
     CDS             36236..40696
                     /gene="gltB"
                     /locus_tag="ACHXOX_000711"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR7004279.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40709..42127
                     /gene="gltD"
                     /locus_tag="ACHXOX_000712"
     CDS             40709..42127
                     /gene="gltD"
                     /locus_tag="ACHXOX_000712"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR7004280.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42311..42715
                     /locus_tag="ACHXOX_000713"
                     /pseudo
     CDS             42311..42715
                     /locus_tag="ACHXOX_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42807..43112
                     /locus_tag="ACHXOX_000714"
     CDS             42807..43112
                     /locus_tag="ACHXOX_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7004281.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43188..43652)
                     /gene="nanQ"
                     /locus_tag="ACHXOX_000715"
     CDS             complement(43188..43652)
                     /gene="nanQ"
                     /locus_tag="ACHXOX_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR7004282.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="ACHXOX_000716"
     CDS             complement(43649..44524)
                     /gene="nanK"
                     /locus_tag="ACHXOX_000716"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR7004283.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44521..45210)
                     /locus_tag="ACHXOX_000717"
     CDS             complement(44521..45210)
                     /locus_tag="ACHXOX_000717"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR7004284.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="ACHXOX_000718"
     CDS             complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="ACHXOX_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR7004285.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="ACHXOX_000719"
     CDS             complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="ACHXOX_000719"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR7004286.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="ACHXOX_000720"
     CDS             complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="ACHXOX_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR7004287.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49044..50366
                     /gene="dcuC"
                     /locus_tag="ACHXOX_000721"
     CDS             49044..50366
                     /gene="dcuC"
                     /locus_tag="ACHXOX_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR7004288.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="ACHXOX_000722"
     CDS             complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="ACHXOX_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR7004289.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="ACHXOX_000723"
     CDS             complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="ACHXOX_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR7004290.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="ACHXOX_000724"
     CDS             complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="ACHXOX_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR7004291.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="ACHXOX_000725"
     CDS             complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="ACHXOX_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR7004292.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="ACHXOX_000726"
     CDS             complement(53000..54127)
                     /gene="zapE"
                     /locus_tag="ACHXOX_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR7004293.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54321..54719
                     /gene="zapG"
                     /locus_tag="ACHXOX_000727"
     CDS             54321..54719
                     /gene="zapG"
                     /locus_tag="ACHXOX_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR7004294.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54873..56240
                     /gene="degQ"
                     /locus_tag="ACHXOX_000728"
     CDS             54873..56240
                     /gene="degQ"
                     /locus_tag="ACHXOX_000728"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR7004295.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56330..57397
                     /gene="degS"
                     /locus_tag="ACHXOX_000729"
     CDS             56330..57397
                     /gene="degS"
                     /locus_tag="ACHXOX_000729"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR7004296.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14259 a        15305 c        14260 g        13607 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg
     3121 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
     3181 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
     3241 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
     3301 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
     3361 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
     3421 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
     3481 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
     3541 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
     3601 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
     3661 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
     3721 gctggctgag tttgttcggt tttcgccggt gcactcagat tttcccattc gctaccttta
     3781 ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc
     3841 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
     3901 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
     3961 agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc gtacataatc
     4021 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
     4081 aatctttccc actgaagtgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
     4141 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
     4201 aaaaggcgac aatcgccgcc tttttagcca gttatttacc gttttaaacg gcttttactg
     4261 catcggcgat acggtgtgca aattcagtta cctgcgcttc gtcttcacct tctaccatca
     4321 cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg ctacccagcg
     4381 ccgcttcaac ttcggcggtc acagctttaa ctgactcatg ctcaagcgga tcgccgctac
     4441 ctgcggtgta acgcacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
     4501 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
     4561 cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac
     4621 gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa
     4681 atggaattcc taactgtttc aacgccagct caagccccat gttactcatc aatgtaccca
     4741 cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
     4801 gatcaccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt
     4861 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
     4921 cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg atagcgataa
     4981 cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcgc
     5041 aatccaccac aatcttcagt tcgctgaggc taagttcgtt cgggaacgtg gctttgcaaa
     5101 actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt tctgccgaat
     5161 caacacagct gatctctttt tccatttccg cttcgatggc ctcttctacc gcatccggta
     5221 gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg ttatgcgatg
     5281 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
     5341 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
     5401 ctgactccag catatagcca gaaatacgcg tgtctttacc aataatgatc ttacgggagc
     5461 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
     5521 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
     5581 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
     5641 tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat gaccgcacag
     5701 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
     5761 tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
     5821 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
     5881 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
     5941 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
     6001 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
     6061 gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc acggatgact
     6121 tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
     6181 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
     6241 gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc
     6301 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
     6361 aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc aaagagtttc
     6421 atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
     6481 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
     6541 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
     6601 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
     6661 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
     6721 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
     6781 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
     6841 gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc gcgtacagca
     6901 gtggacccaa tttctcagag aagccccact gagtcaccat gttacgcgcc aggttggtcg
     6961 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt
     7021 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
     7081 tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
     7141 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
     7201 cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag cgacgttccg
     7261 cacccatcat gattttatct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
     7321 tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt cgccaggtca gcaccggaga
     7381 aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac
     7441 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca accacaacct
     7501 gacggtcgaa acggccagga cgcagcaggg ccgggtcaag aacgtccgga cggttagtcg
     7561 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
     7621 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
     7681 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
     7741 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
     7801 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc gttttaccgg
     7861 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
     7921 ggctcggctc gcgcagatat tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc
     7981 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
     8041 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
     8101 acacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
     8161 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
     8221 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
     8281 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
     8341 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
     8401 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
     8461 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
     8521 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
     8581 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
     8641 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
     8701 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
     8761 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
     8821 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
     8881 cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga
     8941 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
     9001 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
     9061 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
     9121 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
     9181 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
     9241 atatatggga gatggcggta gaatgaccca ttttcaatcc caacgtaagc aaaaaatacg
     9301 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
     9361 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
     9421 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc
     9481 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
     9541 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
     9601 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
     9661 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
     9721 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
     9781 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
     9841 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
     9901 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
     9961 ccaaaaataa cgcgcccatt gttaggcatt ttggtgacat caatcacctg cgcgtttgac
    10021 agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg
    10081 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    10141 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    10201 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    10261 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    10321 aaaaaaatac cgacccaggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    10381 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    10441 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    10501 ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta cattactcaa
    10561 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    10621 attgattacc acagtcagca gatggcgctg cctgccagta cccagaaagt gattactgcg
    10681 ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct tgaaaccaaa
    10741 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    10801 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    10861 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    10921 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc cgccatagtt
    10981 gaccgcaact gtttctccgt ctcgctctac agtgcccaaa aacccggtga tatggccttt
    11041 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt
    11101 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    11161 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    11221 gatggagcca gttatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc
    11281 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    11341 aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggataacatg
    11401 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtacc tggtacatgg
    11461 cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    11521 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    11581 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    11641 ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    11701 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    11761 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc tggctatgca
    11821 gtagaacctg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    11881 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggctt
    11941 cagattattg acaaagtgcg ctttgtttat gccggatgcg gcgtaaacgc cttatccggc
    12001 ctacaaaatc gtgcaaattc aacatattgc aattctcttg taggcctgat aagcgtagcg
    12061 catcaggctg atttggcgtt tatcatcagt gattaacgct tgtaaatgaa ctcaacgcct
    12121 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    12181 tcaagctgct ggcgatgata atcgtcccac atgaattcga ctttctctgg ctgtttcgct
    12241 tcttcagcct gcacgaccgg gttttcaagg ataaaggtca tcacatccca gcagagatct
    12301 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgct
    12361 tcagcaatcg ctttcgcttt ctcttcagct tctgccttat ccagcagatc gatcttattg
    12421 aacactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    12481 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    12541 aacaggacgc ggcaacgttc caggtgcttc aggaagcgga tacccagacc tgcgccttcc
    12601 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    12661 cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta
    12721 gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg
    12781 acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcacccgg cgtaccgttg
    12841 gttttctgcc gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    12901 cagccgccct tagcaaccag cagacgctga ccgtgtttag tcatatcgcc catggtttca
    12961 ccagtaccct ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg
    13021 cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt
    13081 tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg cttccatcca tacgtcacca
    13141 ccatcaccac cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    13201 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    13261 ttcattttat tctccataaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    13321 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    13381 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    13441 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg
    13501 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaat
    13561 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    13621 ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc
    13681 tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc
    13741 gtcgccgcac cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc
    13801 tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca
    13861 acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact
    13921 ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg
    13981 aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt
    14041 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    14101 ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc
    14161 acctgcttca ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga
    14221 atgccaatac ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa
    14281 cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg
    14341 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    14401 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    14461 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg
    14521 atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    14581 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    14641 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatggc
    14701 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    14761 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    14821 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    14881 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    14941 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    15001 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    15061 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    15121 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    15181 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    15241 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    15301 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    15361 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    15421 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt
    15481 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    15541 cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg
    15601 ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt
    15661 tctgcaactg atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat
    15721 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    15781 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    15841 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaatgt
    15901 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    15961 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    16021 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    16081 tgccgaggaa gaagcagaca aagccatcgc agcattacag gtgctcccgg acaccccttg
    16141 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    16201 cttcatcccg cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc
    16261 gctataacac ctgaaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt
    16321 tacaggaact ttttagagca ataggccatc aggagtatag tgatgcccga cagaagaagt
    16381 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    16441 tggcatcccg ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggag
    16501 tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg
    16561 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    16621 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    16681 actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca
    16741 gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca
    16801 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    16861 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg
    16921 ccagaaagtt tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcatgcgcg
    16981 cccatacggc taagctccgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    17041 aatccggtcc cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    17101 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    17161 aggctaatcc agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc
    17221 acggcgtcga gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc
    17281 gccgccacca ggaaagtacc ggtttcgata cgatccggta gcacgcgata gacaccgccg
    17341 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    17401 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcgttttca
    17461 ataatcgtgg taccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg
    17521 ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg
    17581 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    17641 accggacgcg caccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc
    17701 gctaccagcg gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    17761 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    17821 gcacccagct ggcttagcag cttcattgat gtatcgacgt ctttcagttt tgggacgttc
    17881 tggatctcta ccggttcttc cgccagtaga gcggcaaaaa ggataggcag agcagcattt
    17941 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    18001 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    18061 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    18121 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    18181 tttttaaccc gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    18241 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    18301 tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc
    18361 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    18421 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    18481 ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc
    18541 ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    18601 gccagtccac ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc
    18661 accgcttcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    18721 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    18781 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    18841 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    18901 caatcatgtc gtaagcctgc cagtttcccg tctgggagtt tttacgccac tggaagtcca
    18961 gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt
    19021 tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    19081 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag
    19141 caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    19201 cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt
    19261 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    19321 aaggattggt ctggtctgcc tcggttgccg cactcagagg tgcaatcacc agcaaagcga
    19381 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    19441 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    19501 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    19561 tagtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa
    19621 aaccgacgtt taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg
    19681 aactggtatc tggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    19741 aggttttcgg gtccagcgta atatccgcca cccgacccac aacaacgcca ccaatactga
    19801 ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg
    19861 gttcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca
    19921 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    19981 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    20041 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg
    20101 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    20161 accagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca
    20221 atgcctttcc aactgacgcc gaccagagat ccgccccaga tccccacggc gacgaaaata
    20281 accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc
    20341 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    20401 atttctgcgg ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc
    20461 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa
    20521 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaacc
    20581 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca
    20641 tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    20701 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    20761 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    20821 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg
    20881 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    20941 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    21001 gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac
    21061 aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc
    21121 acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc
    21181 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggta gttgggtatg
    21241 ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    21301 ctgaaataac atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg
    21361 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    21421 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    21481 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    21541 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    21601 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg
    21661 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    21721 aatccgttac ggctttctga aaatcttcag cggaccgacg agtatacctg aagaaaggac
    21781 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    21841 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga
    21901 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    21961 ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    22021 tcaatatttt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattccg
    22081 atgttctgcg tcgtgaatta cccttaatgt tgttggtcag cgtagtggcc ggttccgtac
    22141 tctatgacgg acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat
    22201 ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga
    22261 ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc
    22321 tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg
    22381 tgctggcgaa ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg
    22441 gaaccagcct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca
    22501 ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac
    22561 ccgcgctgat aacgccagga gagattgagc cattggcgta cagtcgtgac tacagcgtga
    22621 tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc
    22681 gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt
    22741 tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg
    22801 ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg
    22861 gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt
    22921 aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc
    22981 gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat
    23041 ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa
    23101 tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc
    23161 atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa
    23221 gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg
    23281 gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat
    23341 tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat
    23401 attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgcg
    23461 ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg
    23521 attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt
    23581 cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt
    23641 ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc
    23701 gatggcgacc atttactcgg agtgttacat atgcatgatt tactgcgtgc aggcgtagtg
    23761 taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg
    23821 gccctgtcag cgccgacgtt atagcaaaag cagagaacat tcgtctgctg atcctcgatg
    23881 tcgatggcgt actgtcagat ggcctgattt atatgggcaa taatggcgaa gagctgaaag
    23941 cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg
    24001 ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca
    24061 ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac
    24121 tggcgattgc cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa
    24181 tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg
    24241 ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat
    24301 tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag
    24361 acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca ttgatatggc
    24421 cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga
    24481 gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca
    24541 acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac
    24601 gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa
    24661 tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca
    24721 acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc
    24781 tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg
    24841 caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca
    24901 aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc
    24961 cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat
    25021 ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca
    25081 ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag
    25141 aagtgattga cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac
    25201 ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga
    25261 cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc
    25321 tggtaaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc
    25381 tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg
    25441 gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc
    25501 gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg
    25561 ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg
    25621 atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg
    25681 cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt
    25741 acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag
    25801 accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc
    25861 agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc
    25921 cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca
    25981 ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca
    26041 acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga
    26101 tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg
    26161 gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat
    26221 tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca
    26281 gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca
    26341 gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac
    26401 ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat
    26461 gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc
    26521 cggcaccagc ggtgactaca ttgacgatga gctgccggtc tatcagggcg aaacgacgca
    26581 gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg
    26641 cgctattgct acctctatcg tcgatgccgt tgatgacatc ggttatctga ctgtcccgct
    26701 ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt
    26761 ccttaagcgg atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg
    26821 cctgctaatc caactctccc aattcgataa aaccacgccg tggctggaag aggccagact
    26881 gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt
    26941 cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc
    27001 gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt
    27061 gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca
    27121 aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt
    27181 tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga
    27241 tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg
    27301 tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga
    27361 atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga
    27421 actgaagtat ttcttttcca gtcacatcaa taccgagggc ggcggcgaag cttcctccac
    27481 ggcgattcgt gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag
    27541 cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt
    27601 tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg
    27661 acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca
    27721 ccgaggcact gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc
    27781 gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa
    27841 cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg
    27901 ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa gataaactga
    27961 aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg
    28021 ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag
    28081 gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat
    28141 gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc
    28201 tggcggcgaa acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg
    28261 aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag
    28321 aagatactct gcgcgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg
    28381 ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgtcggca gaccaaacta
    28441 aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc
    28501 gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag
    28561 gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac
    28621 agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc
    28681 gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg
    28741 atctggctcg aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta
    28801 atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct
    28861 caccgcaact cctgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca
    28921 cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag
    28981 aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa
    29041 tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg
    29101 aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt
    29161 acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga
    29221 caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta
    29281 tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta
    29341 gctacttgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag
    29401 agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc
    29461 tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat
    29521 gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct
    29581 gttacgtaat gacgaaggca ccgaggccga agccaacagc gttattgcgc tgctgatgtt
    29641 ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggcccacagg aagaggaagc
    29701 actggccgcc gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact
    29761 ttgacataca actcccttca aactcccccc gacaataaga aaatcacgta ctgaaatcgt
    29821 tctcaatcaa cgtcatttgt acattttgtg cgcttttcac tttcagaaga accttaagaa
    29881 aaccttaaga ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca
    29941 gaaaatattt ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg
    30001 aagacatcgg aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta
    30061 ccatcgtggc gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct
    30121 tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga
    30181 ttgcggaacc ggttatgtct acgatgtgat tgcagatttt gacggcaaac tctccattac
    30241 gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac tgcgccagct
    30301 actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc
    30361 gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg tctgcgacaa
    30421 cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa
    30481 gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa
    30541 agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag
    30601 ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc
    30661 ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg gattaggtaa
    30721 tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata
    30781 acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta
    30841 aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta atccagcttc
    30901 cagccctttt cgtacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg
    30961 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    31021 ctcaatggaa gcgacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat
    31081 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    31141 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    31201 gggaacaggc gcaacactaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    31261 cattaacagc aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    31321 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    31381 ttctgcgcca gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata
    31441 tcatcgacag gacagggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    31501 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    31561 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    31621 aattcacggt caacggtgca ttcgctacca agagtggcaa aattgcttaa attcttcgcc
    31681 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    31741 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacgttgcg cgtttccgtc
    31801 atcgcttcgc cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag
    31861 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    31921 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    31981 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    32041 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    32101 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    32161 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    32221 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    32281 ttcaccgacg ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg
    32341 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    32401 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    32461 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    32521 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    32581 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    32641 aacagaaagc ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc
    32701 tttcagcacg ttagcggtta aggccaccag cggcggtaaa tcctctcgcg gataacgctt
    32761 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagtaacac
    32821 caggtcgtat tcgcccggtt taaacatctc tagcgccgcc ttgccggtca tggcgacatc
    32881 aacgctgtta cctaattttt ccagcacaga acgcgcaaca atcacgttca gttcaatgtc
    32941 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    33001 gacctcttct gctaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg
    33061 ttcgctggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    33121 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat
    33181 ggcgaaaatt ttatccagct catcctgcgg aatgccgata ccagagtctt ccacttcaaa
    33241 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    33301 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    33361 gacctgatgc ggtaatggca gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc
    33421 ctgcaatgcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt
    33481 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt
    33541 ccccagcgtg acggccgaaa catggatggt cttgagatat ttttcctgct cggcggtgag
    33601 ttcggtatcc agcagaatgc ggctcagacc gacgataccg ttcagcggtg tacgcaattc
    33661 gtgactgatg gtggagataa acgtcgtttt gtcgcggctg gcccgttcaa gcgcatcctg
    33721 ataccgctta cgctcggtaa tgtctcgacc aaagcccatc aaaccgtgac gtttacccac
    33781 gcggtcgtag tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc
    33841 cagccactgt tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    33901 gacttttgcg gcggcttccg gtgagtaaac atcagcaggt ttcaggtgaa ccagttgttt
    33961 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    34021 gttacgataa aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    34081 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    34141 ggtttcctgc agttctgctt ccgctttttc acgaacggcg atttcctgat ttagctgggc
    34201 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    34261 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    34321 aaagtagacc gcccacggcg taatcaacaa accaaagaag atagaacgaa taacatcaat
    34381 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    34441 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    34501 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    34561 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    34621 ccctcaacga cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg
    34681 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    34741 agaggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    34801 ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt aatcctccag ttcaataccg
    34861 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcaacgga
    34921 tgtagcttta taccgtcaac accggtttcc actacccgct ccagcgtttg cagacattca
    34981 gccagccctt cgcctggcag accgacaatt aaatgcgagc acaccttcag cccgcgctgg
    35041 cgtgccagct gcgtcgtgcg ctgataacag gcgaaatcat gaccgcgatt aatgcgatgt
    35101 agcgttttgt cgtgcacggt ttgcagcccc agctccagcc acacttcgta gccctggtct
    35161 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg cgtgccgaca
    35221 cacaaaccga caatattggc ctggctcacc gcctgttgat acatcgaacg cagcacctga
    35281 acttcggcaa aggtgctggt atacgcctgg aaataggcga ggtaacgttt cgcgcgatta
    35341 attaaattcg cctgatgcgc cagctgttcg gcaatggaac gatgctgctg cgcttcgtcg
    35401 gcaaacgagg caacattaca gaaggtgcag ccgccgcgcc cgatggtgcc gtcgcgattc
    35461 gggcaactga aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    35521 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    35581 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    35641 cccggcccgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc
    35701 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    35761 aaaaacagta gcatgaaacg tcattaccaa ttaatgcagt ataaaatgct ggttttctcg
    35821 tcaattcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc
    35881 tctaacgact tcccttttag ccttaaagat aaaatctatt ttaatttcag ccatttaata
    35941 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    36001 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    36061 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    36121 ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt cgccacgctc tttctgtacc
    36181 cgtgcgcagc ggttcggtag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    36241 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    36301 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    36361 tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc
    36421 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga aaaactacgc
    36481 cgtcgggatg ctattcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    36541 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    36601 cgaaggcgta ctgggtgaaa tcgccctctc ctctctgccc cgcattgagc aaatttttgt
    36661 gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg
    36721 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tatcgaatct
    36781 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    36841 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    36901 cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa
    36961 caccatcact ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct
    37021 tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc
    37081 gatggataac atgctggaac tcctgctggc aggcgggatg gatatcatcc gcgccatgcg
    37141 tctgttagtg ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    37201 ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc
    37261 cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt
    37321 catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc
    37381 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtta tcgacacccg
    37441 cagtgggcat attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccatccata
    37501 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    37561 agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa
    37621 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    37681 ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat
    37741 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    37801 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    37861 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    37921 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    37981 tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag ctgtgcgaca aagccgaaaa
    38041 aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg ctaaagatcg
    38101 cctgccggtt ccagccccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    38161 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt
    38221 cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca taccttgcct atgaaacgct
    38281 gggccgcctg gtagacaccc atgcgattgc caaagattat cgtaccgtga tgctcaacta
    38341 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    38401 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg
    38461 cctgtgcttc cagggggcgg tcagccgcat tggtggggca agctttgaag acttccagca
    38521 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgt aagcccatca gccagggcgg
    38581 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    38641 gctgcaacaa gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt
    38701 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    38761 ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgctgc
    38821 gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaacag
    38881 catcggcggt aactcaaact ccggtgaagg cggtgaagat ccggcgcgct acggcaccaa
    38941 caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt ggggttactc cggcgtatct
    39001 ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg
    39061 tcagttgccg ggtgataaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg
    39121 agtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgagg acttagcgca
    39181 gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc
    39241 cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac
    39301 catcgcaggc tatgacggtg gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg
    39361 ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg
    39421 tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc ggtgttgata tcatcaaagc
    39481 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg cgctcggctg
    39541 taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg ggtgtggcaa ctcaggatga
    39601 caaactgcgt aagaatcact atcacggcct gccgttcaag gtgacgaatt actttgagtt
    39661 tatcgcccgt gaaacccgcg agctgatggc acagctgggg gtaacgcggc tggtggatct
    39721 gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact
    39781 ggcgctgtcg aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac
    39841 cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa
    39901 accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca ataccgatcg
    39961 ctccgtgggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc
    40021 agccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtatggaa
    40081 cgcgggcggc gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat
    40141 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    40201 aagtattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    40261 ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat
    40321 tggcgacaac ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg
    40381 cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga
    40441 tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg atctggcgat
    40501 ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcatactg gctctcagcg
    40561 cggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc
    40621 gaagtccagt gatgtaaaag cactgctggg tcatcgtagt cgtagcgcag cagagttgcg
    40681 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    40741 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat
    40801 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg
    40861 caacccatac tgcgagtgga aatgtccggt acacaactac atcccgaact ggctgaagct
    40921 cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg caccagacca acaccctgcc
    40981 ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc gaaggttcct gcactctgaa
    41041 cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    41101 cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat
    41161 tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa
    41221 agccgttgtc ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc
    41281 cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat
    41341 tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga
    41401 ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa
    41461 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt
    41521 aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt
    41581 ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt acgtccgtgc gccagggagc
    41641 gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga
    41701 agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat
    41761 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga
    41821 accggatgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    41881 ggcagatgca gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa
    41941 acacagcgtc gagctggatt cacaaggccg catcatcgcc ccggaaggca gcgacaacgc
    42001 cttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    42061 ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac ggtattatga actggctgga
    42121 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    42181 tctttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat
    42241 tgttaccggt agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    42301 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga
    42361 cggtattatt cacctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct
    42421 gatgaccgcg acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga
    42481 ggcaagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag
    42541 gtataagcgt ggtttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    42601 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    42661 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag
    42721 aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc
    42781 atgcaaataa acgaaggaaa gttgccgtga tttacattga acagcttgaa ttaatccata
    42841 aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa atcatcagga aaaactgcct
    42901 tccaccttgt tccattcggc ttaaataaga ctcacgacct tctagaggtg gaagatgctt
    42961 cagaagcaat tcggctggtg attgatgaac gtcattccat tcgctgcagt acacttacag
    43021 caactattac gaataaaaag ggcaagcgaa ttaagcggac aggtatatac agtatcaagg
    43081 gtgttaacat aaaagaatac aacgtcaggt aatggcacag agcggtccgt cagttcaggt
    43141 ttggctctgt gccaaagatg ggtcaggtca cacctgatct aataaattta agccattaaa
    43201 tcagccttaa ccttcaccac caccttttta atctcacccg gctcgccaac aacgcatccc
    43261 ggcttatgcg gctctcctag cataaacacg gcgaacattc ccggtttcag gatgatggct
    43321 tgctcgtttt caatggtgct gcaaagctgg taatcatcct catggtggaa ctcttcacac
    43381 tgacgcgcag tgcctgccat gccaaacaga atccgttctt caccgtttaa taacagctgg
    43441 atatcaatgt attgctcgtg cagctccgct tttttctcaa cgggcgattg agtgttaaac
    43501 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    43561 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    43621 gacggtaatg accgtacttc acccatcatc ataatttttc tccctgggcc aacagcgctg
    43681 ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca tgaaataccg
    43741 ctggctcctg cgccagatac gtttccacca acgccagata cccttctgcc agaccaacgc
    43801 tgccaccgac caccacgcac tggcaatcag ttgtggcttt aatatcagcg atcagccttg
    43861 ccagcacatg tgcggagcgg tgaatcagtt gctgcgcctg ctcgtcgccc tgcccggcgc
    43921 gtgtgaaaat agttctcgca tccgcgccca tgagttcacc ttgcgctgcc gctgcaatac
    43981 cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    44041 gtggatcggc aagcgtatgc ccgatatgcc ccgccagacc gccagggccg gtaagcagtt
    44101 taccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatctcgg
    44161 ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg
    44221 caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag tgtagcaatc
    44281 caccgagatt atgcggattc agcgccagca aactgccgtc gcgaataatt ccggttgaag
    44341 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    44401 gcaaggcttc tggtgtctgg ctggctggcg taggaagttc acgacgatcg cggatctgcc
    44461 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagtgtgg
    44521 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    44581 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc
    44641 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcttcgctca acgttttcac
    44701 cagcgccaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    44761 aataatttcg gcgcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    44821 catcgccagt aaaccgtgat gatgtatacg tgccagcagc gtttcaacag gcaccggacg
    44881 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    44941 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    45001 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    45061 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    45121 atcgagcggg ctgtcaggaa tcggctggca ggagacaatc aggccaccgt tagcagcgat
    45181 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccggtaca
    45241 tagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaat
    45301 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg
    45361 cgcagccaac gctgaacgcg ggaaggcata tccagaccaa tcagcaggat caccacaaat
    45421 gtcaggctga acgagagcga tgccagcgca gtacccagat ccagacgttg agcgatcaac
    45481 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg
    45541 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    45601 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgcg
    45661 ccgccaatcg caaataccgg aataatcagc agctgcgagg ccagcaggct acaaacgtac
    45721 gctttgcggg ttcccagcca gtcaccgagg aagcctccta cgcagcatcc cacggccgcg
    45781 ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc cagatcggtt
    45841 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    45901 accaccatca gcattacgcc cgttggccag cgtttgcctg tactctgcac cataaagctg
    45961 ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    46021 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    46081 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    46141 ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc aaagatgatt
    46201 ggcaaaatgc cgataaagaa cagcgcacgc caaccccaga ccggaaccac caggctatag
    46261 acctgagcgg caacgacggc ccccacagag aagcctgaaa tcaaaaaacc actggctttg
    46321 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    46381 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    46441 ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    46501 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    46561 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccctg tacttcggtg
    46621 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    46681 gaaaatgcgc gccattgtgc acggttgaga tggcgatacc acgggatatt ctgggttgta
    46741 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    46801 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aaacaactca
    46861 cccgcgctct tgcatcaact gctgggccag cgccttcagt tctggcagat atttttcatc
    46921 taccggtcca aacggtttgc ggcacagcgg cacagaaacg acatccatat aatgcaggac
    46981 agttttcagg ccgcggaata cgccagtttt gatcagtaaa tcaatgactt tgttgcactc
    47041 agtttgcagt ttctgcgcgg tctggatatc gccttctttc agcgccttca cgatcccctg
    47101 atatcgccag cccatgatgt tgtaagtact gccgatacca ccatcagcgc ctgccagcaa
    47161 gccggaagcg aagatttcgt cgtaaccgtt atagagcacc agatcaggat gttcacgacg
    47221 gatctgttcc atctgataga gatcgccaga agtctgtttc agcgcaccga cgccaggcaa
    47281 cgtaaccagg gtgttgatct gatccagggt cagttttacc ccactcaggg ctggaatgtt
    47341 gtacacaacc atcggcaaac catccgctga atcaataatt gcccgatagt gatcgcagtg
    47401 ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata
    47461 acgtttagcc gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc
    47521 gatgagttta atcttacctt tcgcctcttc ggcgacgatt tccagtacct gttcacgctc
    47581 ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc acgtataaac cgtcgatgcc
    47641 ctgctgaata ttgaactgaa ccaggcgacg cagactcgct ttatccagtg cttgttgttg
    47701 gtcaaaagga gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc
    47761 tgaagtgata cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa
    47821 gcaacacccc atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct
    47881 ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt
    47941 gcaacgcacg atcggcttcg tcaggatcat gacggcggat cgcatcaacg atcgcaatat
    48001 gctgttgata gctaacgttg ttatgctcgt gcagagcctg atcagccacg gttggacgtg
    48061 cggcgatcag ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttaccaggga
    48121 tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca
    48181 gcgactgact gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcat
    48241 gttcagccgc atagcgcacc agactggatt caaagaacag acgtaattgt tcgaaatggg
    48301 caatcccacc gggatgagaa aggaaatctt tcgccatgcc ggaaaactca ccgatgatag
    48361 tgtccgcaga aggacgcgag acgcgagcgc gttcgccgtt gtttatttgc accagacctt
    48421 tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg cccgacgtta aagaacgcca
    48481 tcagttcgcg ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct
    48541 gttccagctc ttcttccacc atttcggaga gttttttacg cgccagcggg cggctacgca
    48601 agttgcgacc aattgcaggt gaagaatctt cggtttgcga atcaaatgcg ttcataaggc
    48661 ccattctgta aggtcagtgt gattaacatc atcagtgaca tcctatcaca ggattgaaag
    48721 taggggaaaa tggcaggttt ttctctttgt gcctcatcat taccataatt aacggaataa
    48781 ttaactattg cgaaaaatta atgtaacgca gataaacaca tcccgattga attattcata
    48841 agactattca caagcattat gaatattatg aatgtgttct tacaaaataa tcataagcgc
    48901 atatttttta atgaaaaatc acatcaccta caattaaaaa cacgacatcc gcaccataaa
    48961 tagacttgca aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccgtt
    49021 ttcttttgac acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta
    49081 cgatgggcta tttgatcctg aaaaactaca aacctcaggt ggtgctggct gccgcaggtg
    49141 tcttcctgat gatgtgcgga gtctggttag ggttcggtgg tgtactcgat cccgccaaaa
    49201 gcagcggcta cttgatcgtc gacatttata atgaaatcct gcgcatgctg tccaaccgca
    49261 ttgccggatt ggggctgtcg attatggcgg tgggcggtta tgcccgctac atggagcgca
    49321 taggagccag tcgcgcgatg gtgagcttgc taagccgccc gttaaaactc attcgctcgc
    49381 cgtatattat tctgtcggca acttacgtca tcggccaaat catggcgcag tttattacca
    49441 gcgcctccgg tctgggtatg ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg
    49501 gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac gatgtccatt gagtggggga
    49561 ttctggaaac gaactccatt tttgcagccc aggtcgcggg aatgaaaatt gccacatact
    49621 tcttccacta ccagcttccg gtcgcctctt gcgtcattat ctcggtggcg atctcccact
    49681 ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat caatcacgaa caggcagagc
    49741 aaaaaactct cgataatgtc ccgccgctct attacgccat tttacctgtg atgccgttaa
    49801 tcctgatgct cggctcgctg ttcctcgccc acgtcgggct gatgaagtca gaactgcatc
    49861 tggtggtggt gatgttacta agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc
    49921 ataacttgcg cgaaacaatg gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt
    49981 ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga
    50041 ttggcactgt tgatgcggtt atcaggggtg cggagcattc tggtctgggc ggtattggcg
    50101 tgatgattat tatggcgcta gtcattgcca tttgtgccat tgtgatgggc tctggcaatg
    50161 cgccgtttat gtcatttgcc agtcttattc cgaatatcgc agccggacta catgtaccag
    50221 cggttgtaat gattatgccg atgcattttg ccacgacgct agcgcgcgcg gtttcgccga
    50281 ttactgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
    50341 ttgccacaat cacgctattt tattaagtca ttaaaaagac aaaacaggcc gcctgggcct
    50401 gttttgtatt acttcacaac acgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt
    50461 tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata
    50521 acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag
    50581 gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg
    50641 gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc
    50701 tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc
    50761 ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg
    50821 tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga
    50881 cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt
    50941 ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt
    51001 tcagctcttt cgcacccggg ccgctgagct cgatacccag ttgcggcaga cgccacagca
    51061 gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac
    51121 cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt
    51181 cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat
    51241 gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg
    51301 ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat
    51361 ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt
    51421 ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct
    51481 gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag
    51541 cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg
    51601 gccagtcaga agttgtgtta cccaataagg aacgactctc tttgttcgaa aatcaaacaa
    51661 aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg
    51721 accattgtgg tgagtggatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata
    51781 gttgacttag acccttattg gcgatgtggt ttttgttttg cctgtctttc agacggcagc
    51841 aaaaagcaac ttcccatttt ttacgctgat tcagaattta gccataaaaa aacccgccga
    51901 agcgggtttt ttcgtaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac
    51961 tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca
    52021 cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga
    52081 gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg
    52141 gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc
    52201 atgcgggcag tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta
    52261 ccgttgcccg gtttgatgaa aacgcgagct gcggaatttt tgcggcgacc agtgccgtag
    52321 tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt
    52381 gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc
    52441 agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga
    52501 gcaatcatct cttcaaagct cacttgtttg ataccaccga tgtggccggt gtggtgatag
    52561 tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg
    52621 atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgtaggcga
    52681 cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag
    52741 tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa
    52801 tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc
    52861 cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac
    52921 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
    52981 ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt
    53041 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat
    53101 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
    53161 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
    53221 catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc
    53281 gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg
    53341 tcgctaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca
    53401 gcgccaacca tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca
    53461 gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat
    53521 cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc
    53581 gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca
    53641 aggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt
    53701 caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg
    53761 tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca
    53821 ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc
    53881 cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc
    53941 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg
    54001 tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct ttttgaacgt
    54061 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
    54121 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
    54181 aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac
    54241 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
    54301 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
    54361 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
    54421 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
    54481 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
    54541 atcagcacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgct gaagcaaacc
    54601 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
    54661 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
    54721 ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
    54781 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
    54841 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaccccag ctgttgagtg
    54901 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
    54961 caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc
    55021 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
    55081 aagaattcaa aaagtttttt ggtgatgatt taccggagca acctgcacaa cccttcgaag
    55141 gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg
    55201 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
    55261 aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca
    55321 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag
    55381 cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag
    55441 gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta
    55501 accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca
    55561 ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata
    55621 tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag
    55681 gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
    55741 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag
    55801 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
    55861 ctcgtatcgc gaccaccgag ccgggcacaa aagtgaaact tggcctgctg cgtaacggca
    55921 aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa
    55981 tgatcgcgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca
    56041 aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
    56101 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
    56161 aagtgctggc ggcaaaatcg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca
    56221 tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt
    56281 taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa
    56341 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
    56401 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc
    56461 tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg
    56521 tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat
    56581 catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat
    56641 catcgtcgcc ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat ctgactctct
    56701 aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca ttccaatcaa
    56761 tgcacgtcgc gtaccgcata ttggcgacgt ggtactggcg atcggtaacc cgtacaacct
    56821 cgggcagacc attacccagg ggattattag tgccacgggt cgaattggtc tgaacccgac
    56881 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
    56941 gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa
    57001 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat
    57061 tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggca ttggcggacg
    57121 tgagatcgca ccgctgcacg cacagggcgg tggtatagat caactgcaag ggatcgtggt
    57181 taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat
    57241 tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc aggtggcgga
    57301 aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct
    57361 gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac
    57421 cggagtctgt g
//
LOCUS       DAZRVY010000012        56934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZRVY010000012 DAZRVY010000000
VERSION     DAZRVY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.012"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            3..1169
                     /gene="proV"
                     /locus_tag="ACHXOX_000730"
     CDS             3..1169
                     /gene="proV"
                     /locus_tag="ACHXOX_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR7004297.1"
                     /translation="MFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEI
                     FVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLREVRRKKIAMVFQS
                     FALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQR
                     VGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMR
                     IGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLI
                     RKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDA
                     PLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1162..2226
                     /gene="proW"
                     /locus_tag="ACHXOX_000731"
     CDS             1162..2226
                     /gene="proW"
                     /locus_tag="ACHXOX_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR7004298.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2284..3276
                     /gene="proX"
                     /locus_tag="ACHXOX_000732"
     CDS             2284..3276
                     /gene="proX"
                     /locus_tag="ACHXOX_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR7004299.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3567..4751
                     /locus_tag="ACHXOX_000733"
     CDS             3567..4751
                     /locus_tag="ACHXOX_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7004300.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4875..5612
                     /gene="ygaZ"
                     /locus_tag="ACHXOX_000734"
     CDS             4875..5612
                     /gene="ygaZ"
                     /locus_tag="ACHXOX_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR7004301.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5602..5937
                     /gene="ygaH"
                     /locus_tag="ACHXOX_000735"
     CDS             5602..5937
                     /gene="ygaH"
                     /locus_tag="ACHXOX_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR7004302.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6028..6558
                     /gene="emrR"
                     /locus_tag="ACHXOX_000736"
     CDS             6028..6558
                     /gene="emrR"
                     /locus_tag="ACHXOX_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR7004303.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6685..7857
                     /gene="emrA"
                     /locus_tag="ACHXOX_000737"
     CDS             6685..7857
                     /gene="emrA"
                     /locus_tag="ACHXOX_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR7004304.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7874..9412
                     /gene="emrB"
                     /locus_tag="ACHXOX_000738"
     CDS             7874..9412
                     /gene="emrB"
                     /locus_tag="ACHXOX_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR7004305.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9476..9991)
                     /gene="luxS"
                     /locus_tag="ACHXOX_000739"
     CDS             complement(9476..9991)
                     /gene="luxS"
                     /locus_tag="ACHXOX_000739"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR7004306.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10141..11697)
                     /gene="gshA"
                     /locus_tag="ACHXOX_000740"
     CDS             complement(10141..11697)
                     /gene="gshA"
                     /locus_tag="ACHXOX_000740"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR7004307.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(11770..12198)
                     /locus_tag="ACHXOX_000741"
     CDS             complement(11770..12198)
                     /locus_tag="ACHXOX_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR7004308.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12195..12761)
                     /gene="yqaB"
                     /locus_tag="ACHXOX_000742"
     CDS             complement(12195..12761)
                     /gene="yqaB"
                     /locus_tag="ACHXOX_000742"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR7004309.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13042..13118)
                     /locus_tag="ACHXOX_000743"
     tRNA            complement(13042..13118)
                     /locus_tag="ACHXOX_000743"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13082..13084),aa:Arg,seq:acg)
     gene            complement(13317..13393)
                     /locus_tag="ACHXOX_000744"
     tRNA            complement(13317..13393)
                     /locus_tag="ACHXOX_000744"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13357..13359),aa:Arg,seq:acg)
     gene            complement(13457..13533)
                     /locus_tag="ACHXOX_000745"
     tRNA            complement(13457..13533)
                     /locus_tag="ACHXOX_000745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13497..13499),aa:Arg,seq:acg)
     gene            complement(13537..13629)
                     /locus_tag="ACHXOX_000746"
     tRNA            complement(13537..13629)
                     /locus_tag="ACHXOX_000746"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13593..13595),aa:Ser,seq:gct)
     gene            complement(13945..14130)
                     /gene="csrA"
                     /locus_tag="ACHXOX_000747"
     CDS             complement(13945..14130)
                     /gene="csrA"
                     /locus_tag="ACHXOX_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR7004310.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14365..16995)
                     /gene="alaS"
                     /locus_tag="ACHXOX_000748"
     CDS             complement(14365..16995)
                     /gene="alaS"
                     /locus_tag="ACHXOX_000748"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR7004311.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17123..17623)
                     /gene="recX"
                     /locus_tag="ACHXOX_000749"
     CDS             complement(17123..17623)
                     /gene="recX"
                     /locus_tag="ACHXOX_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR7004312.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(17851..18912)
                     /gene="recA"
                     /locus_tag="ACHXOX_000750"
     CDS             complement(17851..18912)
                     /gene="recA"
                     /locus_tag="ACHXOX_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR7004313.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(18992..19489)
                     /gene="pncC"
                     /locus_tag="ACHXOX_000751"
     CDS             complement(18992..19489)
                     /gene="pncC"
                     /locus_tag="ACHXOX_000751"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR7004314.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19634..20719)
                     /gene="mltB"
                     /locus_tag="ACHXOX_000752"
     CDS             complement(19634..20719)
                     /gene="mltB"
                     /locus_tag="ACHXOX_000752"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR7004315.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            20975..21538
                     /gene="srlA"
                     /locus_tag="ACHXOX_000753"
                     /gene_synonym="gutA"
     CDS             20975..21538
                     /gene="srlA"
                     /locus_tag="ACHXOX_000753"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR7004316.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21535..22494
                     /gene="srlE"
                     /locus_tag="ACHXOX_000754"
                     /gene_synonym="gutE"
     CDS             21535..22494
                     /gene="srlE"
                     /locus_tag="ACHXOX_000754"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR7004317.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            22505..22876
                     /gene="srlB"
                     /locus_tag="ACHXOX_000755"
                     /gene_synonym="gutB"
     CDS             22505..22876
                     /gene="srlB"
                     /locus_tag="ACHXOX_000755"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR7004318.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            22880..23648
                     /gene="srlD"
                     /locus_tag="ACHXOX_000756"
                     /pseudo
     CDS             22880..23648
                     /gene="srlD"
                     /locus_tag="ACHXOX_000756"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            23754..24113
                     /gene="gutM"
                     /locus_tag="ACHXOX_000757"
     CDS             23754..24113
                     /gene="gutM"
                     /locus_tag="ACHXOX_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR7004319.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24180..24953
                     /gene="srlR"
                     /locus_tag="ACHXOX_000758"
                     /gene_synonym="gutR"
     CDS             24180..24953
                     /gene="srlR"
                     /locus_tag="ACHXOX_000758"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR7004320.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            24946..25911
                     /gene="gutQ"
                     /locus_tag="ACHXOX_000759"
     CDS             24946..25911
                     /gene="gutQ"
                     /locus_tag="ACHXOX_000759"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR7004321.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(25908..27422)
                     /gene="norR"
                     /locus_tag="ACHXOX_000760"
     CDS             complement(25908..27422)
                     /gene="norR"
                     /locus_tag="ACHXOX_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR7004322.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            27609..29048
                     /gene="norV"
                     /locus_tag="ACHXOX_000761"
     CDS             27609..29048
                     /gene="norV"
                     /locus_tag="ACHXOX_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR7004323.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29045..30178
                     /gene="norW"
                     /locus_tag="ACHXOX_000762"
     CDS             29045..30178
                     /gene="norW"
                     /locus_tag="ACHXOX_000762"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR7004324.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30306..32558)
                     /gene="hypF"
                     /locus_tag="ACHXOX_000763"
     CDS             complement(30306..32558)
                     /gene="hypF"
                     /locus_tag="ACHXOX_000763"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR7004325.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(32711..33238)
                     /gene="hydN"
                     /locus_tag="ACHXOX_000764"
     CDS             complement(32711..33238)
                     /gene="hydN"
                     /locus_tag="ACHXOX_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR7004326.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33386..34396)
                     /gene="ascG"
                     /locus_tag="ACHXOX_000765"
     CDS             complement(33386..34396)
                     /gene="ascG"
                     /locus_tag="ACHXOX_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR7004327.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            34656..36113
                     /gene="ascF"
                     /locus_tag="ACHXOX_000766"
     CDS             34656..36113
                     /gene="ascF"
                     /locus_tag="ACHXOX_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR7004328.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            36122..37546
                     /gene="ascB"
                     /locus_tag="ACHXOX_000767"
     CDS             36122..37546
                     /gene="ascB"
                     /locus_tag="ACHXOX_000767"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR7004329.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(37660..38079)
                     /locus_tag="ACHXOX_000768"
     CDS             complement(37660..38079)
                     /locus_tag="ACHXOX_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7004330.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38076..38387)
                     /locus_tag="ACHXOX_000769"
     CDS             complement(38076..38387)
                     /locus_tag="ACHXOX_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR7004331.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(38549..39322)
                     /locus_tag="ACHXOX_000770"
     CDS             complement(38549..39322)
                     /locus_tag="ACHXOX_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004332.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(39347..39817)
                     /gene="hycI"
                     /locus_tag="ACHXOX_000771"
     CDS             complement(39347..39817)
                     /gene="hycI"
                     /locus_tag="ACHXOX_000771"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR7004333.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(39810..40220)
                     /gene="hycH"
                     /locus_tag="ACHXOX_000772"
     CDS             complement(39810..40220)
                     /gene="hycH"
                     /locus_tag="ACHXOX_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR7004334.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40217..40984)
                     /gene="hycG"
                     /locus_tag="ACHXOX_000773"
     CDS             complement(40217..40984)
                     /gene="hycG"
                     /locus_tag="ACHXOX_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR7004335.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(40984..41526)
                     /gene="hycF"
                     /locus_tag="ACHXOX_000774"
     CDS             complement(40984..41526)
                     /gene="hycF"
                     /locus_tag="ACHXOX_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR7004336.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41536..43233)
                     /gene="hycE"
                     /locus_tag="ACHXOX_000775"
     CDS             complement(41536..43233)
                     /gene="hycE"
                     /locus_tag="ACHXOX_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR7004337.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(43251..44174)
                     /gene="hycD"
                     /locus_tag="ACHXOX_000776"
     CDS             complement(43251..44174)
                     /gene="hycD"
                     /locus_tag="ACHXOX_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR7004338.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(44177..46003)
                     /gene="hycC"
                     /locus_tag="ACHXOX_000777"
     CDS             complement(44177..46003)
                     /gene="hycC"
                     /locus_tag="ACHXOX_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR7004339.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46000..46611)
                     /gene="hycB"
                     /locus_tag="ACHXOX_000778"
     CDS             complement(46000..46611)
                     /gene="hycB"
                     /locus_tag="ACHXOX_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR7004340.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46736..47197)
                     /gene="hycA"
                     /locus_tag="ACHXOX_000779"
     CDS             complement(46736..47197)
                     /gene="hycA"
                     /locus_tag="ACHXOX_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR7004341.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47409..47759
                     /gene="hypA"
                     /locus_tag="ACHXOX_000780"
     CDS             47409..47759
                     /gene="hypA"
                     /locus_tag="ACHXOX_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR7004342.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            47763..48635
                     /gene="hypB"
                     /locus_tag="ACHXOX_000781"
     CDS             47763..48635
                     /gene="hypB"
                     /locus_tag="ACHXOX_000781"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR7004343.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48626..48898
                     /gene="hypC"
                     /locus_tag="ACHXOX_000782"
     CDS             48626..48898
                     /gene="hypC"
                     /locus_tag="ACHXOX_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR7004344.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            48898..50019
                     /gene="hypD"
                     /locus_tag="ACHXOX_000783"
     CDS             48898..50019
                     /gene="hypD"
                     /locus_tag="ACHXOX_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR7004345.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            50016..51026
                     /gene="hypE"
                     /locus_tag="ACHXOX_000784"
     CDS             50016..51026
                     /gene="hypE"
                     /locus_tag="ACHXOX_000784"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR7004346.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51100..53177
                     /gene="flhA"
                     /locus_tag="ACHXOX_000785"
                     /pseudo
     CDS             51100..53177
                     /gene="flhA"
                     /locus_tag="ACHXOX_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(53214..53567)
                     /locus_tag="ACHXOX_000786"
     CDS             complement(53214..53567)
                     /locus_tag="ACHXOX_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR7004347.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(53644..53778)
                     /locus_tag="ACHXOX_000787"
     CDS             complement(53644..53778)
                     /locus_tag="ACHXOX_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004348.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            53854..56415
                     /gene="mutS"
                     /locus_tag="ACHXOX_000788"
     CDS             53854..56415
                     /gene="mutS"
                     /locus_tag="ACHXOX_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR7004349.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            56521..56934
                     /locus_tag="ACHXOX_000789"
                     /pseudo
     CDS             56521..56934
                     /locus_tag="ACHXOX_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        13393 a        15493 c        14790 g        13258 t
ORIGIN      
        1 aaatatttgg tgagcatcca cagcgagcat tcaaatatat cgaacaagga ctttcaaaag
       61 aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt ctggccattg
      121 aagaaggcga gatatttgtc atcatgggat tatccggctc gggtaaatcc acaatggtac
      181 gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat ggtgtggata
      241 ttgccaaaat atccgatgcc aaactccgtg aggtgcgcag aaaaaagatt gcgatggtct
      301 tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg ttcggtatgg
      361 aattggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca ctgcgtcagg
      421 tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg atgcgtcaac
      481 gtgtgggatt agcccgcgcg ttagcgatta atccggatat tttattaatg gacgaagcct
      541 tctcggcgct cgatccatta attcgcaccg agatgcagga tgagctggta aaattacagg
      601 cgaaacatca gcgcaccatt gtctttattt cccacgatct cgatgaagcc atgcgtattg
      661 gcgaccgaat tgccattatg caaaatggtg aagtggtaca ggtcggcaca ccggatgaaa
      721 ttctcaataa tccggcgaat gattatgtcc gtaccttctt ccgtggcgtt gatattagtc
      781 aggtattcag tgcgaaagat attgcccgcc ggacaccgaa tggcttaatt cgtaaaaccc
      841 ctggcttcgg cccacgttcg gcactgaaat tattgcagga tgaagatcgc gaatatggct
      901 acgttatcga acgcggtaat aagtttgtcg gcgcagtctc catcgattcg cttaaaaccg
      961 cgttaacgca gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta gcagtcgatg
     1021 cacaaacgcc tcttagcgag ttgctctctc atgtcggaca ggcaccctgt gcggtgcccg
     1081 tggtcgacga ggaccaacag tatgtcggca tcatttcgaa aggaatgctg ctgcgcgctt
     1141 tagatcgtga gggggtaaat aatggctgat caaaataatc cgtgggatac cacgccagcg
     1201 gcggacagtg ccgcgcaatc cgcagacgcc tggggtacac cgacgactgc accgactgac
     1261 ggcggtggtg ctgactggct gaccagtacg cctgcgccaa acgtcgagca ttttaatatt
     1321 ctcgatccgt tccataaaac gctgatcccg ctcgacagtt gggtcactga agggatcgac
     1381 tgggttgtta cccatttccg tcccgtcttc cagggcgtgc gcgttccggt tgattatatc
     1441 ctcaacagtt tccagcaatt gctgctgggt atgccagcgc cagtggcgat tatcgttttc
     1501 gctctcatcg cctggcagat ttccggggtc ggaatgggcg tggcgacgct ggtttcgctg
     1561 attgccatcg gcgcaatcgg tgcctggtcg caggcaatgg tgactctggc gctggtgtta
     1621 accgccctgc tgttctgtat cgtcatcggt ttgccgttgg ggatatggct ggcgagaagt
     1681 ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca tgcagaccac gccagcgttt
     1741 gtttatctgg taccaatcgt catgctgttt ggtatcggta acgtgccggg cgtagtggtg
     1801 acgatcatct ttgctctgcc gccgattatc cgtctgacga ttctggggat taaccaggtt
     1861 ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca gatgctgttc
     1921 aaagttcagt taccgctggc gatgccgacc attatggcgg gcgttaacca gacgctgatg
     1981 ctggctcttt ctatggtggt catcgcctcg atgattgccg tcggcgggct gggtcagatg
     2041 gtacttcgcg gtatcggtcg tctggatatg ggccttgcca ccgttggcgg cgtcgggatt
     2101 gtgatcctcg ccattatcct cgaccgcctg acgcaggccg ttgggcgcga ctcacgcagt
     2161 cgcggcaacc gtcgctggta caccactggc cctgtcggtc tgctgacccg cccattcatt
     2221 aagtaactct gcacttgccc ggtaacgccg ggcattatca ccctgccaaa aaaaggaata
     2281 acaatgcgac atagcgtact ttttgcgaca gcgtttgcca cgcttatctc tacacaaact
     2341 tttgcagccg atctgccggg caaaggcatt actgttaatc cagttcagag caccatcact
     2401 gaagaaacct tccagacgct gctggtcagt cgtgcactgg agaaattagg ttataccgtc
     2461 aataaaccca gcgaagtgga ttacaacgtt ggctacacct cacttgcttc cggcgatgca
     2521 accttcaccg ccgtgaactg gacgccgctg catgacaaca tgtacgaagc tgccggtggc
     2581 gataagaaat tttattgtga aggggtattc gttaacggcg cggcacaggg ttacctgatc
     2641 gataagaaaa ccgccgacca gtacaaaatc accaacatcg cacaactgaa agatccgaag
     2701 atcgccaaac tgttcgatac caacggcgac ggtaaagcgg atttaaccgg ctgtaaccca
     2761 ggctggggct gcgaaggtgc gatcaaccac cagcttgccg cgtatgaact gaccaacacc
     2821 gtgacgcata atcaggggaa ctacgcagcg atgatggccg acaccatcag tcgctacaaa
     2881 gagggcaaac cggtgtttta ttacacctgg acgccgtact gggtgagtaa tgagctgaag
     2941 ccagggaaag atgtggtctg gttgcaggtg ccgttctccg cactgccggg cgataaaaac
     3001 gccgatacca aactgccgaa tggtgcgaat tatggcttcc cggtcagcac catgcatatc
     3061 gttgccaaca aagcctgggc cgagaaaaac ccggcagcag cgaaactttt tgccattatg
     3121 cagttgccag tggcagatat taacgcccag aacgccatta tgcatgacgg caaagcctca
     3181 gaaggcgata ttcagggcca tgttgatggt tggatcaaag cccaccagca gcagttcgat
     3241 ggctgggtga atgaggcgct ggcagcgcag aagtaatttt tattcgggcg gataagtcgt
     3301 ttttgcctca tccgccattc tgtgcacaat gcctgatgcg acgctggcgc gtcttatcat
     3361 gcctacaaaa tacactcatt ccgtatggcg gataaggcgt tttcgccgca tccgccgttc
     3421 tgtgcacaat gcctgatgcg acgctggcgc gtcttatcat gcctacaaac cacatcgcac
     3481 atttcaacaa gctatctttc gcccccattc atcaacatcc gctattattg atttccagct
     3541 taatcatcac ctgatgaaca aaaataatga ctaaacctaa tcatgagctt agcccggcgc
     3601 tgatcgtgct gatgtctatc gccaccggtc aggcggtagc cagtaactat tacgcccagc
     3661 cattgctcga caccatcgcg cgtaactttt ccctttccgc cagttcggca ggctttattg
     3721 ttaccgccgc gcagttgggc tatgcagcag gtctgctgtt tcttgttccc ctcggtgata
     3781 tgtttgaacg ccgccgcctg attgtctcga tgaccttact ggcggcaggc ggcatgttaa
     3841 tcactgccag cagtcagtcg ctggcgatga tgatcctcgg tacggcatta accggtttat
     3901 tctcagtcgt ggcacaaatt ctggttccgc tggcagcgac gctggcttca ccggataaac
     3961 gcggcaaagt ggttggcacc attatgagcg gtctgctgtt ggggatcttg ctggcacgaa
     4021 ccgttgccgg gttactggca aacctcggcg gctggcgcac cgtcttttgg gttgcctcgg
     4081 tgttaatggc actgatggcg ctagcattat ggcgtggtct gccacaaatg aaatcagaaa
     4141 cccacctcaa ctacccacag ttgttaggtt ccgttttcag catgtttatc agcgataaga
     4201 tcctccgcac ccgcgcattg ctgggctgcc tgacctttgc caacttcagc attctctgga
     4261 cctcaatggc ctttttgctt gccgctccac cttttaacta cagcgatggt gtcattggtc
     4321 tgtttggact tgcgggagct gccggagcgt tgggcgctcg tccggcgggc ggttttgccg
     4381 ataagggtaa atcgcaccac accacaactt tcggtctgct gctgctatta ctttcatggc
     4441 tggcgatctg gtttggtcac acttccgtac tggcgctgat tatcggcatc ctggtgctgg
     4501 atctcaccgt acagggcgtg catatcacta accagacggt aatttatcga atacaccctg
     4561 atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc ggtgccgccg
     4621 gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt tgtctggctg
     4681 gcgcgacgat tgccctagtt aacttactgg tctggtggcg aggttttcat cgtcaggaag
     4741 ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg gtgttaagaa
     4801 gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat taacaatcgt
     4861 taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg gcgaccttca
     4921 tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg gcctttgcgt
     4981 tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt ttctcctgca
     5041 tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc gggagtagtt
     5101 tgtgggttgc tgcactgacc gtcatggcaa tggatgttcg ccatgtgttg tatggcccgt
     5161 cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgccctg tgggcgtttg
     5221 gcctgacgga tgaggttttt gccgccgcaa cagcaaaact ggtacgcaat aatcgccgct
     5281 ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg gtatttggta
     5341 cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc gttgaagcag
     5401 cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct ttccagcgca
     5461 aacaatctct ttgcgttacc gcagcgttag ttggtgccct tgcaggcgta acgctatttt
     5521 ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg ttaatccagg
     5581 cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt actggttggc
     5641 gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa tgcccgccca
     5701 accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc gatatgcgct
     5761 ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt cgtgcccacg
     5821 ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat tatcatccca
     5881 acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat tatataaccc
     5941 acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg ttactatatc
     6001 ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat tgaacaaatg
     6061 ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct tctgactcgt
     6121 ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct gaaggctcag
     6181 gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga aaaccacagt
     6241 attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc gacgcgtatt
     6301 gccgatgaac tggaaaaacg cggttggatc gaacgtcgtg aaagcgataa cgatcgccgc
     6361 tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt tttaccaccg
     6421 cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa agatcagctc
     6481 gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga cggtgtggtt
     6541 ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata aaagaaaaaa
     6601 tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct cagccgatga
     6661 gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac cccgcagcaa
     6721 ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt gctctttata
     6781 attattgccg tagcgatagg gatttattgt tttttggtac tgcgtcattt cgaagaaacc
     6841 gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc tggcagcgtg
     6901 acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgacgtgct ggtcactctc
     6961 gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc ttccagcgtt
     7021 cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat tgaggtgcag
     7081 aaaatcgccc tcgcaaaagc acaaagcgac tacaaccgcc gtgtgccgct gggcaatgcc
     7141 aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag tgcccaggcg
     7201 caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct ggggactaaa
     7261 ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc ctggctggcg
     7321 ctggagcgta cccgtattgt cagtccgatg accggttatg tctcccgccg cgcggtacag
     7381 cctggggcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc caccaatatg
     7441 tgggtggatg ccaactttaa agagacgcag attgccaata tgcgtatcgg tcagccggtc
     7501 actatcacca cggatattta cggcgatgat gtgaaataca ccggtaaagt ggttggtctg
     7561 gatatgggca caggtagcgc attctcactg cttccggcgc aaaatgcgac cggtaactgg
     7621 atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca gctggagcaa
     7681 tacccgctgc gcattggttt gtccacactg gtgagcgtca ataccactaa ccgtgacggt
     7741 caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc gcgtgaaatc
     7801 agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc tggctaatcc
     7861 agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg tcattatgac
     7921 gattgcgctg tcactggcga cattcatgca ggtgctggac tccaccattg ctaacgtggc
     7981 gatccccact atcgccggga atctgggctc atcgctcagc cagggaacgt gggtaatcac
     8041 ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg caaagcgcgt
     8101 cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt cgtgggcgtg
     8161 tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg ggattgtcgc
     8221 cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc cagccaaacg
     8281 ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt gcggcccgat
     8341 cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca tcaacgtgcc
     8401 gattggcgtg gcggtggtgt tgatgacact gcaaactctg cgcggacgtg aaacccgcac
     8461 cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta tcggcagcct
     8521 gcagattatg ctcgaccgcg gtaaagagct ggactggttt tcatcacagg aaattatcat
     8581 ccttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc tgaccgacga
     8641 taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg gctgcttgtg
     8701 tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc agttgttgca
     8761 ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg tagggattat
     8821 tccggtgatc ctgtcgccga ttatcggccg cttcgcgcat aaactggata tgcggcggct
     8881 ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct atacctttga
     8941 accaggtatg gattttggcg cgtcggcctg gccgcagttt atccaggggt ttgcggtggc
     9001 ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg aacgactggc
     9061 ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg gcacgtcgat
     9121 aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga ctgagtcggt
     9181 aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc ttgggatgac
     9241 gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc tgattatttc
     9301 cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg ggctggtgtg
     9361 gtttgctaaa ccgccatttg gcgcaggtgg cggtggcggc ggtgcgcact aagtacaact
     9421 aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt actgactaga
     9481 tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg ttgatgcgta
     9541 cgtcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag tgcatctggt
     9601 aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc actttcagca
     9661 cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc gtaccaatca
     9721 gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc tctacaccat
     9781 taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta tggatccctc
     9841 tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg gtgattgcgt
     9901 cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc atccgggtat
     9961 gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt tttttaaaaa
    10021 ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc atttgttatc
    10081 atcatccctg aattcagaga tgaaattttg gccactcacg agtggccttt ttcttttctg
    10141 tcaggtgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt ccatttcctg
    10201 ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca gaatttccag
    10261 cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg ttccgccaat
    10321 accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg gattatcgaa
    10381 gcaggcaacc agttcatcac acactttctg atacgcgtcg ccgccgttaa tgctatccag
    10441 cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca gtaacgggaa
    10501 ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt caaggatcac
    10561 ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg gtgcatcagc
    10621 cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat ctacaccaat
    10681 cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc cgccacgtaa
    10741 cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa tcggcgcata
    10801 cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac cgtctttctc
    10861 aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa ggcccgctac
    10921 gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat tggtatagcc
    10981 gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc actcggtttt
    11041 ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg ccggagatgc
    11101 accaaacaga taaggaatga cccaaccgaa acgatagtaa ttgcggataa cgcggaaata
    11161 gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact tcgcttgcca
    11221 gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca tcagcgcgcc
    11281 gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt tagaagtgcc
    11341 gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac ttaacggcca
    11401 catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca taaaggtcag
    11461 catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt ccgcaaaatc
    11521 ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac ctgttgttgc
    11581 cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct gtatcccctt
    11641 taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg ggatcaaatt
    11701 gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac catatgtgca
    11761 ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa ctgcaacata
    11821 gcgtaacgct ttaccaaggc acaaaaaaaa gattaccggt ccccacgaga tgcgcatcca
    11881 tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata atagcgtgac
    11941 tgcaccatag cgtttcagcc agccgatagc tttctcttgc cagcgcgatg ttttacgcaa
    12001 tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac ccattgttgc
    12061 tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca ccagcacgac
    12121 ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca acgagaaaag
    12181 cgataacgct tcactcacag caagcgaacg tccacagcgt ccatacctgc ggcacgggcc
    12241 gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg cacgcccata
    12301 cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac gtgatcggca
    12361 gcgacgacgg cgtcaaaata atggcgtaat cccaggtgcg ccagcaatgc ctcagcgatg
    12421 gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact tttcaccaca
    12481 tcaacaagag gaagcggttc gacgctatcc agcagcatac ttcttactgc ttctgttttt
    12541 tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat tgcctgagca
    12601 atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta ctgaagaccg
    12661 tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gctccgtatc caggattgtg
    12721 ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa
    12781 aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga aggagaagaa
    12841 aattcaggaa atggataaag gagttattta gaaatgagat atttttgaag gaaatttttt
    12901 ggagaagatg gtgcatccgg gaggatgact cactgacgtt cgcccttcgg gccgttgctg
    12961 gcgcaacgtt atccttcctg gtgcctgcgg ttaataccgc tatctcttcg ataccttcat
    13021 tgctgaaggt tacggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt
    13081 tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaaact tattctactg
    13141 ctgatattga taccgctact aaagccatat caattaagag atggtgcatc cgggaggatt
    13201 actcgccttg cggctcgccc ttcgggccgt tgctaaagca acgttatcat ccctggtgcc
    13261 tgcggttaat tccgctacct cttcgatacc ttcattgctg aagatttcgg agaatatggt
    13321 gcatccggga ggattcgaac ctccgaccgc tcggttcgta gccgagtact ctatccagct
    13381 gagctacgga tgcatcggga aaatttattt tactgctgat attgataccg ctactaaagc
    13441 catatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    13501 gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg ggattcgaac
    13561 cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca gcctctcggc
    13621 cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct catcgtcgct
    13681 gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc cacactttta
    13741 tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt gttttatcta
    13801 cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact taagttgaat
    13861 gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg tgcgtctcac
    13921 cgataaagat gagacgcgga aagattagta actggactgc tgggattttt cagcctggat
    13981 acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta cgccaatacg
    14041 tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa tcatgagggt
    14101 ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt aaaagagtcg
    14161 ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc aatgacaaac
    14221 acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta gccgatatac
    14281 acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg tgaagcgagt
    14341 ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc ctttcacact
    14401 ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca tgtcaggacg
    14461 tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc ctgctttcac
    14521 acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt cggctaccgt
    14581 tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca tggtacgcaa
    14641 cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat taacatcaat
    14701 tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa gctgttgtag
    14761 ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg ccagattatt
    14821 gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg catgaacggt
    14881 ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag cagtacccga
    14941 ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag taccgccaca
    15001 cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat acttctcgcc
    15061 gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga tccatgatgt tggtttcgat
    15121 cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa tctcttccgg
    15181 tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa ccagtgaacc
    15241 tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca ggtgcgttgc
    15301 ggagtgattc agacgaatac gggcgcgacg agcctcatca acatcagcct gcacagcgtc
    15361 gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct ggccgtattt
    15421 ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt tatcaccaac
    15481 ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag cttcctggcc
    15541 tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt tgccgttcag
    15601 ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca ttgcgttgta
    15661 atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca ttgctgcttc
    15721 aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg tcaggtcaac
    15781 cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg tatcaccaga
    15841 aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac gggcaaactg
    15901 ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca ggtcttcacc
    15961 cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg tttctttcgc
    16021 gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta cataaccacg
    16081 gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac gaatgtggtc
    16141 agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt tcgctaccgc
    16201 ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt gcagcaccgc
    16261 agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg gttccatcgt
    16321 gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa tgtagcggtc
    16381 gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt gatcgtagaa
    16441 gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt tgtcagatgc
    16501 gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga tccctacttc
    16561 tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc acagacgctc
    16621 tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact gaatggcatc
    16681 gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg tatggtgacg
    16741 cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca cgcagcgttg
    16801 ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat ccttgaactg
    16861 gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca gggagctgct
    16921 ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac ggatctcagc
    16981 ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca ccagctccag
    17041 cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt ttcttgagtg
    17101 ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc tggatatctt
    17161 ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct gaaaagacag
    17221 ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg caccagtgcg caccagtcga
    17281 tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc tgattcagtt
    17341 cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata aagcgcgcaa
    17401 caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg cgctcgtaat
    17461 cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt gccgcgagtt
    17521 tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc gcacgatcca
    17581 acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat ccttacaact
    17641 taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag atttaatagt
    17701 caatgagtgt gcagccaatt cgttagagga tcgaggtgag agtaattttt cacgccagtg
    17761 ttggcctgtt cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgtgcac
    17821 acattatcta ttatcatcaa taacgaacga ttaaaaatct tcgttagttt ctgctacgcc
    17881 ttcgctgtca tctacagaga aatccggcgt tgagttcggg ttgctcagca gcaactcacg
    17941 tactttcttc tcgatctctt tcgcggtttc cgggttatct ttcagccagg cagttgcatt
    18001 cgctttaccc tgaccgatct tctcaccttt gtagctgtac cacgcgcctg ctttctcgat
    18061 cagcttctct tttacgccca ggtcaaccag ttcgccgtag aagttgatac cttcgccgta
    18121 gaggatctga aattcagcct gtttaaacgg tgcagcgatt ttgttcttca ccactttcac
    18181 gcgggtttcg ctacccacca cgttttcgcc ctctttcacc gcgccgatac gacggatgtc
    18241 gagacgaaca gaggcgtaga atttcagcgc gttaccaccg gtagtggttt ccgggttacc
    18301 gaacatcaca ccaattttca tacggatctg gttgatgaag atcagcagcg tgttggactg
    18361 cttcaggtta cccgccagct tacgcatcgc ctggctcatc atacgtgccg caaggcccat
    18421 gtgagagtcg ccgatttcgc cttcgatttc cgctttcggc gtcagtgccg ccacggagtc
    18481 aacgacgata acgtctactg cgccagaacg cgccagggcg tcacagattt ccagtgcctg
    18541 ctcgccggtg tccggctggg agcacagcag gttatcgata tcgacgccca gtttacgtgc
    18601 gtagattggg tccagcgcgt gttcagcatc gataaacgca caggttttac cttcacgctg
    18661 cgctgcggcg atcacctgca gcgtcagcgt ggttttaccg gaagattccg gtccgtagat
    18721 ttcgacgata cggcccatcg gcagaccacc tgccccaagc gcgatatcca gtgaaagcga
    18781 accggtagag atggtttcca catccatgga acggtcttca cccaggcgca tgatggagcc
    18841 tttaccaaat tgtttctcaa tctggcccag tgctgccgcc aacgctttct gtttgttttc
    18901 gtcgatagcc atttttactc ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt
    18961 tgaagcaatt atactgtata ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca
    19021 aggtctgcaa tgcatacgca gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc
    19081 attcccgccg ggtaatgcct tcaccgcggg cagtggcaaa agcaaaccag acggtgccga
    19141 caggcttctc ttcactgccg ccatccggcc cggcgatacc actaatagac acggcataat
    19201 cagcacgagc cgctttcagt gcacctatcg ccatttccac cacgacgggt tcactcaccg
    19261 cgccatgctg cgccagcgtc tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac
    19321 tgtaggtgac aaatccgcgt tcaaaccagg cggagctacc ggcaatatcg gtaatcactt
    19381 tcgctaccca accaccggta caagactcgg cagttgttac ggttgcgcca cgggctttca
    19441 gcgcctgccc aacctgttcg cttaactgca tcagttcact gtcagtcatc acaacctcag
    19501 tcagtcaaaa atttatgccg gacaagatag cactttcgtc aggaagatgg tgttggcgtt
    19561 gcaattttac gaggaggatt cagaaaaaag ctgattagcc agagggaagc tcacgccccc
    19621 ctcttgcaaa tagttactgc acgcgcgcca gcgccacggc ttgtcctaac tgccagaccg
    19681 ccattgcgta atgggtgctg tggttataac gggtaatggt gtagaagttc ggcagaccgt
    19741 accagtactg gtaaccggta ccgacatcca gacgcagcag gctggcttgt tgatggttgc
    19801 ccagcggctg ctgtggcgtt aaacctgccg tggcaagctg cgaaatgctg tacctggttt
    19861 tgaagccatt tggcaagcct ggagcctgac cgtttgccat taccgcgacc tgatcgcctt
    19921 tcacccagcc gtgcgctttg aaatagttcg ccacactacc gatcgcatca accggatccc
    19981 acaggttgat atgcccgtcg ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca
    20041 taaactgtcc gtagcccatc gccccggcaa aggaaccttt cagattgagc ggatcgtcct
    20101 gctcgtcgcg cgccatcaac aggaaggttt ccagctcgcc agagaaatac tccgcgcggc
    20161 gtgggtagtt aaatgacagc gttgccagcg catcgaggat gcgagttttc cccatcacgc
    20221 gcccccagcg ggtttcaacg ccgataatcc cgacgataat ttccggcggt acgccataca
    20281 cctgccacgc gcgattcaac gcatcttcat actgattcca gaaaaccaca ccgttctgca
    20341 cgttgtccgg cgtaataaat tttttgcgat aacgcagcca tgcgccgttc ggacctgatg
    20401 gcggtttcac cgatgtggtt ggtgcctggt tatccatcag ccgcagtacc gaatccagac
    20461 gcttcgcctg ggagagaatt tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca
    20521 ttctgtcgat gaactgctgg gcattcgggt tattagcgaa atcgccgccc atctgcatca
    20581 cattgtgctg aggctcaagc aggaagccgc cagacggcgt tccggtggtc gtttcagtct
    20641 cagtaggttt tggcttgctg ctacaggcgg caagcaacac aaaaagggga agcaatgtta
    20701 cataacgacg cttgaacatg aggggtccat ttaacagatt caaccagggg caagtatggt
    20761 aaagcatcac gccccgcaca aggaagcggt agtcactgcc cgatacggac tttacataac
    20821 tcaactcatt cccctcgcta tccttttatt caaactttca aattaaaata tttatctttc
    20881 attttgcgat caaaataaca cttttaaatc tttcaatctg attagattag gttgccgttt
    20941 ggtaataaaa caataaatcc tgaaggagag aacaatgata gaaaccatta ctcatggtgc
    21001 agagtggttt atcgggctgt tccaaaaggg cggagaggtg tttaccggga tggtgaccgg
    21061 cattcttccg ctgttgatta gcctgctggt tatcatgaac gcactgatta attttatcgg
    21121 tcagcatcgt attgaacgtt ttgctcaacg ttgcgccggt aaccctgttt cccgttacct
    21181 gctgctaccg tgcattggca cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg
    21241 ctttatgccg gaaaagtaca aacccagcta ctacgcggca gcctcttata gctgccactc
    21301 aatgaatggc ctctttcccc atatcaaccc tggcgaactg tttgtttatc ttggcattgc
    21361 cagcggtctg acaacgctga acctgccact tggcccactg gcggtgagct atctgctggt
    21421 tggtctggtc accaacttct ttcgcggctg ggtgaccgat ctgaccaccg ccatttttga
    21481 gaaaaagatg ggcattcaac ttgaacaaaa agttcacctg gcaggagcaa catcatgacg
    21541 cgtattcgga tcgaaaaagg aacgggtggc tggggcggcc cgcttgagct ggaagccacg
    21601 ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg
    21661 gcacaactta ctggctggca ggctattgac ggatttaaag aaggtgaacc cgcggaggcg
    21721 gaaattggtg tcgcggtaat cgactgtggc ggcacattac gctgcggcat ctatccgaaa
    21781 cgacgtattc ccaccattaa tatccactcg acgggcaagt ccagtccgct ggcgcagtac
    21841 attgtggaag atatttatgt ctctggcgta aaagaagaaa acatcactgt agtgggtgat
    21901 gcgacaccac aaccctcttc cgtgggccgt gactatgaca ccagcaagaa aatcaccgaa
    21961 caaagcgatg gtttactggc gaaggtggga atgggtatgg gttctgccgt tgccgtgttg
    22021 tttcaatctg gtcgtgacac catcgacact gtattaaaaa ccattctgcc gtttatggcg
    22081 ttcgtctcgg cgctcattgg catcattatg gcttctggcc ttggtgactg gattgcccac
    22141 ggtcttgctc cgctggcgag ccatccactg ggtctggtca tgctggcgct catctgctcc
    22201 ttcccgctgc tttcaccttt cctcggccca ggcgcagtta tcgcacaggt tatcggcgta
    22261 ttgattggcg tgcagattgg tctcggcaat attccgccgc atctggcttt accggcactg
    22321 tttgccatca acgcgcaggc ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa
    22381 gcccgtcagg acacggttcg cgtcggtgtc ccttctgtac tggtgagcca ctttttaacc
    22441 ggcgcaccga ctgtactgat cgcctggttt gtctccggtt ttatctatca atagaggctg
    22501 aaacatgacc gttatttatc agaccaccat cacccgtatc ggcgcgagtg ccactgacgc
    22561 cctcagcgac cagatgctca tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta
    22621 ttgcttcatt cattgccacg gcgagttgaa aggtgcactc catcccggtt tgcaatttac
    22681 gctcgggcag catcgctatc cggtgaccgc tgttggcagc gtggcggaag acaaccttcg
    22741 cgaactgggt catgtcaccc tgcgcttcga tggtttaaac gaagcggaat ttccgggcac
    22801 tgtccatgtg gcaggccctg tacccgacga tatcgcgccg ggatcggttt tgaagtttga
    22861 atctgttaag gagtaaaaaa tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt
    22921 aggcgcgttc ctgtgccacg gtctggctgc cgaggggtat cgcgtcgcgg ttgtcgatat
    22981 tcagagcgac aaagccgcaa atgtggcaca agaaattaac gccgaatatg gtgaaggaac
    23041 agcgtatagt tttggtgctg acgccaccag cgagcaaagc gtcctggcgc tctctcgtgg
    23101 ggtagatgaa atctttggtc gcgtggattt gctggtctac agcgccggaa tagcaaaagc
    23161 agcctttatc agcgacttcc agctcggcga ttttgaccgt gaatctggtg ggttatttcc
    23221 tgtgtgcccg tgaattttct cgtttgatga tccgcgacgg gattcagggt cgcattattc
    23281 agatcaactc gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga
    23341 aatttggtgg cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta
    23401 cggtgcattc actgatgctc ggtaacctgc tgaaatcgcc tatgttccag tcactgttac
    23461 cccaatacgc gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca
    23521 aagtaccgct caaacgcggc tgcgattatc aggatgtgct gaatatgctg ctgctctatg
    23581 ccagtcctaa ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga
    23641 tgttctaatc aacagcggag atccgttaag gatctccgtg agactaatag aatgcctgat
    23701 gcgctacgct catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat
    23761 ccgcactcat caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc
    23821 aaatttctcg ttttaaccgt gccttcgaca cactatgcca gcaagggcgg gttggcgtgg
    23881 gccgttccag cgggcgcttt aaaccacggg tcgtggtcgc catcgccctg gacgatcagc
    23941 agcgcatcgt cgacaccttg tttatgaaag gactgaccgt cttcgcacga ccgcaaaaaa
    24001 ttcccgcaat taccggtatg catgcgggtg atttacagcc cgatgtgatc tttccccatg
    24061 atccactatc acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt
    24121 gtgaatgtta cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca
    24181 tgaaacctcg tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct
    24241 cggttgaaga attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg
    24301 tcattctgga acatgcagga accgtcattc gtacttatgg cggagtggtg ttgaataaag
    24361 aggaatccga tccgcctatc gatcataaaa cactcatcaa cacccacaag aaagagctga
    24421 tagcagaagc tgccgttagt tttattcatg atggcgattc gatcattctt gatgctggca
    24481 gtaccgtttt gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca
    24541 acagtctgca tatcgtcaat gcgctatctg aactggataa cgaacaaact atcctgatgc
    24601 caggcggaac gtttcgtaaa aaatcggcct catttcacgg acagctggca gagaatgcct
    24661 tcgaacattt cagtttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg
    24721 gcgtaaccac ctttaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg
    24781 aagtgatttt gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca
    24841 gtcttgaaag cgtcgataag ctgattaccg acgcaggtat cgatccggcg tttcgtcagg
    24901 cgctggaaga gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta
    24961 ctgaacgcgg gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa
    25021 cgtctgggcg atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg
    25081 gtggtttcgg gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc
    25141 agtaccggca catcggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg
    25201 atgattgaaa gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg
    25261 gatctgatta ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa
    25321 ccgacgtcac cgctgggcct ggcggcaaaa gcggtgctgg atatctccgt agaacgcgaa
    25381 gcctgcccga tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac
    25441 gcgctggcga tggcggtcat gcaggcgcgc ggatttaatg aagaagattt tgcccgctcc
    25501 cacccagccg gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgccgt
    25561 gacgatgcca tcccacaggt ggcgttaacc gccagcgtga tggatgcgat gctggaactc
    25621 agccgcaccg gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc
    25681 tttaccgacg gcgatttacg tcgctggctg gtaggcggcg gcgcactcac cacgccagtt
    25741 aatgaagcga tgacgacagg cggcaccacg ttacaggcgc aaagtcgcgc catcgacgcc
    25801 aaagagatcc tgatgaagcg caaaatcact gccgcaccgg tggtggatga aaacggcaaa
    25861 ctcaccggcg caattaacct gcaggatttc tatcaggccg ggattattta atccttcatt
    25921 cccagacgtt tcgccagccg atgcaggttg gcgacgtcgg tttccagcat ccgcgcgcag
    25981 gcagcccagt tatgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct
    26041 tctgtcgctt cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc
    26101 aacgtcacct caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg
    26161 gtggctctcg ccagcactac cgcccgatga atagcatgtt ccagttcgcg cacgttcccc
    26221 ggaaaacgat agtgttgcag taaatttcgc gctccggcac ttaataccac gcgggagagc
    26281 cccagccgca aacgacactg ctcgcagaaa taccccgcca gcagaatgac atcatcgccc
    26341 cgctcacgca gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caagtcagcg
    26401 cgaaatcgcc ctgccatcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc
    26461 acatcgaccc gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc
    26521 ctcagcagct tggcctgcaa tgccaacgac aactcgccga tctcatccag aaacagagtg
    26581 ccgttatccg ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct
    26641 ttcacatgcc cgaacaactc actttccgcc acactttccg gcagtgcagc acagttgaga
    26701 tagaccagcg gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc
    26761 ttaccggttc ccgtctcacc gctgatcagg acgttgagat cggacgccgc cacaatctca
    26821 atctcttttt tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc
    26881 gcttcaaacg gcgtggcatc gcctggcatc atattctggc tttccagttg ttcaatcagc
    26941 aacgcattgc ttaacgctcc cgccgccagc gcagcaatca gccgtagctc ttcgtcgctg
    27001 aaaacatcga actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgc
    27061 ccggcaaaca atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc
    27121 aaaccgtcat agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg
    27181 cgggcaatcg cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacatcc
    27241 tttgccagac cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca
    27301 gacgcatcgc actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga
    27361 tgaccaatcc cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc
    27421 atctttgcct cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag
    27481 tcattttgac tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa
    27541 aaaactggca cgtaatctgc aattagcaag acatcttttt agaacacgct gaataaattg
    27601 aggttgctat gtctattgtg gtgaaaaata acattcattg ggttggtcaa cgtgactggg
    27661 aagtgcgtga ttttcacggt acggaatata aaacgctacg cggcagcagt tacaacagct
    27721 acctcatccg cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg
    27781 aatttgtgca gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta
    27841 accatgcaga agaggaccac gctggggcgc tgaccgaact gatggcgcaa attcccgata
    27901 cgccgattta ctgtactgcc aacgctatcg actcgataaa tggtcatcat catcatccgg
    27961 agtggaattt taatgtggtg aaaactggtg acacgctgga tatcggcaac ggcaaacagc
    28021 tcatttttgt cgaaacgcca atgctgcact ggccggacag catgatgact tacctgacag
    28081 gcgacgcggt gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt
    28141 tcaacgatga agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata
    28201 tcctgacgcc gttcagccgc ctggtaacac cgaaaattac cgagatcctg ggctttaact
    28261 taccagtcga tatgatagcg acctctcacg gcgtggtatg gcgcgataac ccgacgcaaa
    28321 ttgtcgagct gtacctgaaa tgggcggctg attatcagga agacagaatc accattttct
    28381 acgacaccat gtcgaataac acccgcatga tggctgacgc tatcgcccag gggattgcgg
    28441 aaaccgaccc acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa
    28501 tcctgaccaa tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg
    28561 tgatgatgcc gaaaatcgcc ggtctggtgg aggagatgac cggattacgc ttccgtaaca
    28621 aacgcgccag tgctttcggc tctcacggct ggagcggcgg tgcggtggat cgtctttcca
    28681 cgcgcctgca ggatgcgggt ttcgaaatgt cgcttagcct gaaagccaaa tggcgaccag
    28741 accaggacgc tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc
    28801 tcgcgccgct gccgcagagc acggtgaata cggtggttaa agaagaaacc tctgccacca
    28861 cgaagtccga cctcggccca cggatgcagt gcagcgtctg ccagtggatt tacgatccgg
    28921 caaaaggcga gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gttccggata
    28981 acttcctctg cccggaatgc tctctcggca aagacgtctt tgacgaactg gcatcggagg
    29041 caaaatgagc aacggcattg tgatcattgg ttcgggcttc gccgcccgcc aactggtgaa
    29101 aaatattcgc aaacaggacg ccagtattcc attaaccctg atcgccgccg acagcatgga
    29161 tgagtacaac aaaccggacc tcagccatgt tatcagccag gggcaacgtg ccgacgacct
    29221 tacccgccag acggcgggtg aatttgccgg gcagtttaat ctgcacctgt ttccgcacac
    29281 ctgggtgacg gatatcgatg ccgaagccca tgtggtgaaa agccagaata atcagtggca
    29341 atacgacaag ctggtgctgg caactggtgc cagtgccttt gtcccgccag tgcctgggcg
    29401 tgagttaatg ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg
    29461 ggatgcccga cgcgtgttga ttgtcggcgg tggtttgatt ggtagcgaac tggcgatgga
    29521 tttttgtcgt gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc
    29581 attaatgcca ccagaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca
    29641 tctgctgttg aaatctcagt tgcaggggct ggaaaaaacg gattctggca ttctggcaac
    29701 gctggaccac cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc
    29761 ggaaacagcc ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtcgatag
    29821 ttatctgcaa accagtaatg acgatattta tgcgctgggc gattgcgcgg aaatcaacgg
    29881 tcaggtactg ccattcctgc agccgattca acttagcgcg atggtgctgg caaaaaatct
    29941 tctcggcaat aacacgccgc tgaaactacc ggcgatgctg gtgaaaatca aaacgccgga
    30001 attaccgctg catctggcag gcgaaaccca gcgtcaggat ttacgctggc aaattaatac
    30061 cgaacgccag ggaatggtgg ctcgcggtgt tgacgatgct gaccagcttc gcgcctttgt
    30121 ggtcagtgag gatcggatga aagaggcatt tggattgttg aaaacattgc cgatgtaggt
    30181 gggctactgt gcctaaaatg tcggatgcga cgctggcgcg tcttatccga cctacgggga
    30241 cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac
    30301 acgttttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc
    30361 cccagagaca aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca
    30421 gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat
    30481 accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc
    30541 gccagcgcat catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca gttcagccac
    30601 tgctgccaga aagcggcgag atccagttga ttgtccacca gcggcatcgt caccggatgc
    30661 gtcactccgt ggcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa
    30721 cttaacgtgg ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac
    30781 gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt
    30841 tgctgttgca cacttgctgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac
    30901 tgcgccagca ggtttcgcca cggctgcttc gctgccaaat cgccacccgg aagcgccact
    30961 gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc
    31021 cacaaagcgc cgttctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg
    31081 ccatccaacg gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt
    31141 accgtttgcg tcggcagatt catttcacgc gcccactggc tggagacata gcccggatgc
    31201 gcgtcatgca caacgtattg cggggtaaaa tcgtagatgt tttgcatcag gcgtaacgct
    31261 tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc
    31321 gcttgttcac cgcgcaccag gcagaaggta tttttcagat ccgcgccgag acacagcaca
    31381 ggcggaacat ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa
    31441 cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg
    31501 cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg
    31561 ctgatagctg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt
    31621 tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg
    31681 tcaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc
    31741 gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac
    31801 atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta
    31861 ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg
    31921 cccattttta actgtgcgat agccgcctgt aatgccgcct cttgttctgc atgttcacca
    31981 tgacttaccc attcaagatg tgggccacac tccgggcagg ccaccggctg ggcgtggaag
    32041 cgacgatcga gcgggtcacg gtactctttg tcacaagccg gacacaacgg aaacgccgcc
    32101 atcacggtaa acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg cgggccgcag
    32161 tgggtacagt tgataaacgg ataacgataa cgccgttcgc ctggggtatt catttcggca
    32221 aggcaagcag ggcaagtagc ggcatccggg acaatttgcg tattcatggc acctcccgcg
    32281 ctctggcgga tggtgaactc agtgggcagt tgtgaccaga tgtacggctc acgctcgacg
    32341 ctatcaatac gcgccagcgg cgggcagtgc tgatgcaatt gaacaagtaa cgtttccggg
    32401 tcttcccgca gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt
    32461 aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga
    32521 atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga
    32581 attgcgtaac ccgtcctgcc ggacacgact gcgtcgtatc cggcagtctc aggtcggcga
    32641 taccgccagc tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc
    32701 tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcactc agttgttcaa
    32761 gtttattacg atcgacacaa atcagcgcat gagtcgggca agccgccata cacgccgggc
    32821 cgtcttcacg atggttgcac aggtcacatt tattggcttc ggctttgtca gcccgtacat
    32881 tcagccccgc gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg
    32941 ggcaagccac aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc
    33001 ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact
    33061 gacggcaaac tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa
    33121 atgtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca
    33181 cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca
    33241 aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc agttttaatt
    33301 cactgttatt taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag
    33361 tgacgatgac atgtgatgac aatgtttatc gcgaaagagc aatgagtgag tcgcggcgga
    33421 tcagttttcc gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca
    33481 gccgtccaat aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg
    33541 gaacgatata gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca
    33601 ccgctacgcc gcgctcgtgc aacgctttca tcgcacctat cgccatatcg tcgttactgg
    33661 caactaacgc gctaaattta gccccacgtt cgagcaacgt ttctacccct tcggccccgc
    33721 tggcgggcgt ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg
    33781 ccagcgcgtc tttataccca gcaagacgtt caatgctggt gggggaatcc atcgagccgg
    33841 taaggaaagc aatctcctga tgtccggcgt ttatcaactc tgccacggca ttaaagctgg
    33901 tctgtttatg atcgcaccag acgctatggc tgctgttttt gcgcaggcgg cgattaagca
    33961 ccattatcgg ctgactgtgc gcgtcaatga tgtcatcgat ctcatccacg cttaaaaaac
    34021 gcgggtaaat cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct
    34081 cttcttcggc gctgtgttta ccatctgcca atagcaactg ccgccctttc tcttccgcca
    34141 ttcgcgcggc atgaaagagt aattcactaa aataaatgcc gtggtaaagc gtgttggtca
    34201 ctaccaaccc cagcgtctga gtactcttcg ccgacagatt gcgcgccagc aagtttgggc
    34261 ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc
    34321 cattacctga aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt
    34381 ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg
    34441 tttgccgatt gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat
    34501 ataaaaaatg aacaattaac tctcttgctt atttagtgac aactattcat gattttgtga
    34561 aaccggtttc ctaattccgt ttcagcatcg gcatttttcc gttacgtcga ctgataacaa
    34621 ctacatctac cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg
    34681 cacgctcggt gatagcggca ctgggcggcg ttgataacat ctcggcggtc acgcactgta
    34741 tgacgcgttt gcgcgttgtt atcaaagatg accagcttat cgacagcccg acgttaaaaa
    34801 ccatccccgg cgtgctcggc gtggtacgta gtgacaacca gtgtcaggtg attatcggca
    34861 ataccgtttc acaagccttt caggaagtcg tcagcctgct gccgggagat atgcagcccg
    34921 cacagcccgt gggtaaaccc aaactcacgc tgcgtcgcat tggtgcgggg atcctcgatg
    34981 cgctgatcgg caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac
    35041 tgctggcgat gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca
    35101 ttctgaatgt gattggtgac ggtgctttct tcttcctgcc gctgatggtc gcggcatctg
    35161 ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc catcgcgggt gtgctggtac
    35221 atccgagttt tattgaactg atggcgaaag cggcccaggg tgaacatgtc gaatttgccc
    35281 tgattccggt caccgcggtg aaatacacct acacagtgat cccggcgctg gtgatgacct
    35341 ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac accggcggtg accaaaaact
    35401 tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc
    35461 cgattggtat ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc
    35521 tgggctggct ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga
    35581 tgcaccgcgt ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga
    35641 tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg
    35701 cgtggaaaac gaaaaacccg gaactgcgcc agacggcgct ggctgcggcg gcatcagcca
    35761 ttatggcggg gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc
    35821 ttatcgccag tcttatcagc ggttttattt gcggcgcggt tgccggtatg gcggggcttg
    35881 ccagccactc aatggcagcg ccagggttat ttaccagcgt gcaattcttc gatccggcga
    35941 atccaatgag catcgtctgg gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta
    36001 tcctcacact gttgctcggc tttgaggata ttcctgttga ggaagcggct gccgaggcgc
    36061 gaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa
    36121 aatgtcagta tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga
    36181 aggtgcgttc cgtgaaggtg gcaaagggct gaccactgtc gatatgatcc cacacggcga
    36241 gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt
    36301 ttatcccagc catgaggcga cggattttta tcatcgttat aaagaagata tcgccctgat
    36361 ggcagagatg ggattcaagg ttttccgtac ctcaattgcc tggagccgtc tctttccgca
    36421 gggcgatgaa atcacgccca atcagcaggg cattgctttt tatcgttctg tctttgaaga
    36481 gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc cacttcgatg tgccgatgca
    36541 tctggtcacc gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta
    36601 cgcccggacc tgctttgaag catttgatgg tctggtgaaa tactggctta ccttcaatga
    36661 aatcaacatt atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga
    36721 aaatcaggat caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc
    36781 caccaaaatc gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg
    36841 taacttctac ccttacagtt gcaagccgga agatgtctgg gcggcgctgg agaaagaccg
    36901 ggaaaacctg ttttttatcg atgtgcaggc gcggggcacg tatccggctt actctgcccg
    36961 cgtattccgc gaaaaagggg taaccatcga caaagcaccg ggcgatgatg aaatcctgaa
    37021 aaacaccgtc gattttgtct ctttcagcta ttacgcctcg cgctgcgcct cggcggagat
    37081 gaacgccaac aatagcagtg cggcgaacgt agtgaaatcg ctgcgtaacc cgtatctgca
    37141 agtgagcgac tggggctggg gtattgatcc actcggtctg cgtatcacca tgaacatgat
    37201 gtacgaccgt tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga
    37261 acttgctgcc aatggcgaga ttaacgatga ctatcgcatc agctatttac gcgaacatat
    37321 ccgcgcaatg ggcgaagcga ttgcagatgg cattccgctg atgggctaca ccacctgggg
    37381 ctgtattgat ttagtctccg cctctacggg tgaaatgagc aaacgctacg gttttgtcta
    37441 cgtagaccgt gacgacgcag gcaacggcac gctgacgcgc acacgtaaga aatcgttctg
    37501 gtggtataaa aaagtgattg ccagtaatgg ggaagattta gagtaggtaa cagtgccgga
    37561 tgcggcgtga acgccttatc cggcctacat gtcgcacgaa ccaggaaggc ccgataagcg
    37621 cagcgcatcg ggcaatctgg ggtttggcat cagtcaaaat caaagaaacc attccggctt
    37681 aacgccaaat cgggctgaaa gtgccttaat gtgggatatc gtcagggatc gttgtccaga
    37741 caaaatctga ctgaccaggg atttagagcc gatttcgttc ttcaaatccg cataggtcag
    37801 attatgttga tcaatcagcg tgcgaagtaa cgccacgcca acaggcattg ccgcaacggc
    37861 tttgtcgaac tctgcaaact cttggttgtt gttttcatat tctgcaatgc ggctcgccag
    37921 aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa tattcgacca gttccagtgc
    37981 ttcacgataa tcatcttctg agtcactacc tcccagaaag gggactgcat tgaccagttc
    38041 tcttgttgct ttaacagcgc gtgcagcatt agcagtcatt ctttgttctc tctgtagtaa
    38101 cgtgtcagtt tgtcatactc ggcgtgatta gttatgtgct tcacaaagaa ccgcttatta
    38161 acaaagttta tgtaggcaat cacccttagg ttgttcccac ctacatccag aacgtaccat
    38221 ttgttgcgat atttaaaatt gtcgaggttg ggaaacgccg ttcgcatctc ttctggcgta
    38281 gaaaaatccg tttccttaat aacccgatac aatgcctgaa gagccagagc gtcgttagga
    38341 tatttacgcg cagactcttc aaatggcgct ttagaaatga tgtgcatttt taagttttaa
    38401 cttccttata gtaagtaaac agccacatct gcgagttcta tccttatgtg ttcggaaact
    38461 atagctcatc aaatccaagt tcacaatatg taaacacttg agcgcgatat aattaccccc
    38521 aaaatcctca tagccactct gcaaaaagtc agttctggtt caccccatca agaacatccc
    38581 cctttatcaa gcaacgctgc gtctgcgcca gatcttccgt ttgcgggtcg cgcccggcga
    38641 tgggtttgag gacgagttta cggttggggg tataagtaaa acgcgtgagc gcgggatagc
    38701 ttaacttgcc aaaccagccg tgaacacgct gccatttggc gtcttttacc tgatacagac
    38761 tggttatcca gtaaccggaa tcaaactgca tatccagcaa ttgcgtgtga ccattgcctt
    38821 gcaaatccag caaatcatct acgccagttt tgcttgcggt gtagaatccc ctgggttcaa
    38881 agacacaggg acggccttcg cgcgtgaacg tcataagaat taaatgtgca taaggagcca
    38941 gaccattacc ggtacttggt aaccagataa ccagatcctg aatgccgtta cgatcaagat
    39001 cggcgcgata gagttgccct ttgcccgcta tgtttgccag catacccaca ggcacagacc
    39061 aggatttacc ggcgcgatct ttaccgttca attgccattc actatcatca tttggcatct
    39121 tttcaagcgt cacgccagca acgcccactt ttctaaaatg ccaggtttgc tcggcatcat
    39181 aaggcatggt caggagaggt ttcagcgccc gcttttgcgc gtattcgctg tcactcattg
    39241 gcagatattc ggtaaagctg gtggcctgtg caggcagcac taagctcaat aaaaaagcgc
    39301 acatccctgt tctgatattc atttcgctga attccctgat ggagaattat tcttcttcca
    39361 ccgctaactg cgcgaagccg ccattccctt cccagccttc cagtcgttga taaacggttt
    39421 ctaccgcatc tttaatcggc tgagtcatcg ggtagtaaaa gccgacgata tccggctgga
    39481 tgccgaggaa aatcacttcg ccaatgtctt ctttcaactg gtcgataaga tagttcagcg
    39541 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
    39601 tttcgccggg gtttagcccc atatcagtag cgtcaacaat cagtaaccgc gtagggcgca
    39661 gttcacggat ggcgacgatg tcgttttccg gtgcactacc gccatcaatc accacccagt
    39721 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg gccagcgcca tcatcgccca
    39781 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
    39841 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattccttc
    39901 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg
    39961 ttaatgtcga tgacgatctc accatatttc ggcacgcctt ccattttgcg gcgggcttca
    40021 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccacgggca ggtgagcgcc
    40081 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
    40141 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
    40201 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
    40261 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
    40321 cctgccctaa ctgtgtcagg aagtcatcgg caatctgacg accgtaacga taacccgcca
    40381 ggcggcgtgc ttcgcgatcc actttcacac gcagcggctg caccatatcg ccatgcagga
    40441 tttccgcctg ttgttcatcc tgctcacccg ggccacgggc gtgaattttc tgctccagca
    40501 ggccgagcgc cattgcaaag ccgtacagcg tggcggcagg cgttggcggg caaccaggaa
    40561 tgtagacatc taccgggacg attttatccg taccgcccca cacgcagtag agatcgtgga
    40621 aaataccacc gctgttaccg caggcaccgt aggagataca aattttcggg tccggcgcgg
    40681 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
    40741 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcaaaca
    40801 gcggcgaaag cgtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
    40861 gataaacgta ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
    40921 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
    40981 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
    41041 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
    41101 gtcgccgttg tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg
    41161 acgattgcag acgcggcagt tgcacagcgc gaagcgggac tgttgcagga agtcttcttt
    41221 cttccacact gccagttcgt actcttgcga cagtttgatc gccgccgtcg ggcaaacttc
    41281 ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac tcccacgcca gctcaccggt
    41341 ggcgaggtca gtttcaaccg ttaaggcgtt tgacgggcag gcattgacgc aggccgcaca
    41401 gccgatgcac tgctgcgggt tctgctccgg cttaccacgg aagtttttat caaccgcaat
    41461 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    41521 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    41581 ttctttgtac ggcaccactt tgctcttttt cttacgcaca tcgaccacgg tcatgcggtc
    41641 ggtacaggag tagcaagggt ccaagctacc gataatcagc ggcgcatcgg aaacggtgtt
    41701 accgcgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    41761 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    41821 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    41881 accgcccggc aggttatcca gaccgtagtc gatcatgttc agcgcggtat aaacttcgtt
    41941 gatacgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    42001 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcgt gaccgctggc
    42061 gcgaaccatc gggccaacgt tactgaaatc gcgggccatt tccgggtcca gacgaccaat
    42121 gccgacggtg cgctgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    42181 ttcacgacgc atctgttgcg ccagctggcg ggtctggatc atgtcatctt tcaacagatc
    42241 gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg tgaggatctc
    42301 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcaaagcc
    42361 ggtaaagtgg caggcgagac cgaggttgag cagatgcgag tgcaggcgtt ccacctccag
    42421 cagaatggcg cggatcatct gcgcacgttc tggcacctga atacccatcg cgttttctac
    42481 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    42541 gaaggtcact tcgttgtaac ccatacgggt ttccgccagt ttttccatgc cgcgatgaac
    42601 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    42661 gcccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    42721 gcccagttcg ttgatgaact cgtaggtttc agcatcggtg gtcggtgccg gacgctgacg
    42781 ataatccatg ctgtctttac gcagcggata aagttcatcc ggcagcacca gacggcgttc
    42841 atccggcaga ccaaccggaa tcaaaccgta catatcgcgc acttcgcgct cgccccacac
    42901 cgccgccgga acgcgcggcg tcacggacgg atactccggt ttgttggcat caacttcgac
    42961 gcgaaccgtt acccagcact tagtcccctt ctccatcgac agcacgtaat aaacggcgta
    43021 atgaccattc agtttgcgtt cgtcgttacc aaaaagcacc gacaaccagc ccccctgctt
    43081 gtagtagaga aactccacca cttccggcag atagttcacc tttacggtga cagtcagctg
    43141 atctttggtc tgccaggcgt ggtccagcac gacgcccgga aatgcctcat tcagcgcggc
    43201 gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc
    43261 agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag tccaggtaat gcgcggagta
    43321 atatcaagac gcagacgggc catgctgttt tcgaacagcg cgataaccag gacgccgacg
    43381 accagtttga cgatggcaat caccagcgcc agcagcagcc caccggcggt gaaggtttcc
    43441 atctgtcccc acgggataaa tacgccgacg aacatctgca gcaccaccag ctgtttcagg
    43501 ctgatacccc atttcatgac gccaaagccg ctgccgctgt attcagagag cgggccttcc
    43561 tgcagctcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaggtg
    43621 gcgaacgcac aggcacaaag cgccagtacc agcgggatgc tctggctcag cggccagtga
    43681 taaacggtgt cggtgatgtt gctgatgttg gtggaaccgg caacctgtgc ggcaacccac
    43741 agaccaagca gcagcatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcgccg
    43801 atagcggtaa acgggctacc ggtatccaga ccagaaatgg caaagaagaa acgcgcgatg
    43861 gcaaagagat acagtaaggt gattaaatca cccagttgcg gcagcggaga accgacggtc
    43921 accaccggca gcgcagtagc gatagtcagc atgacgccca ccatcacata cggcgtcagg
    43981 cggaacaccc agccggaggc atccgggcca acgctctggc ggcccagcag tttgaaaatg
    44041 tcacgatact cctgcaacac acccggccca cgacggttat gcagacgggc gcgcgccacg
    44101 cgggtaatac cggagagcag cggcgcaacg gcaaataaca ccagcgcctg aattaacgga
    44161 tataaaacac tcattctcag gctcctcgtg aaacaataat caccaccagt accgccagtt
    44221 caaccagcgc catccggcgg aacagcaacg cactaccttc gcactgccag cccggcacca
    44281 gcgacaccgg attcagccat ttgcgcagtt tcagcactgg cgcaaacgcc tgtttaaccg
    44341 gcatggcaaa accgtgagcg gtaatcacca ttgatttttc atggtcgtag ccgcacaccc
    44401 aggccgcacc gcgggaacgt gacggcaggc ggtcgccttt gcaaatcgcc ataatgatga
    44461 atggcagcag cgggcaggca atcagcagca atgtgatcat tggttgagaa acggtggtgt
    44521 tagcaggctc cagaggcaga ggtacagcag cagagagcat cggcagcagc cacggcgcag
    44581 caacaccgcc aattacgcag caaatcgcca gtgccactac gcttacgctc atcaggagcg
    44641 gcgcacaggt ggcgttttcg gcttctttgg tacgcggcgc gccgaggaac gtgacgccat
    44701 agactttcgc catacacatc accgccagcg caccggtaat tgccagaccc acagcgagca
    44761 gcggccccag cagacgggca acaaacgcgc cgctattgct cagtttgaag aaggattgat
    44821 agataaccca ttccccggca aacccgttca gtggcggcag cgcagccatt gccatcaacc
    44881 cgactaacat ggcgatggag ataaccggca ttttcttgcc aataccaccg agtttttcga
    44941 tatcgcgatg accggtacgg aaccagacgc tccccgcccc gaggaacagt acgcttttga
    45001 acaggctatg gttaagcaga tggtacagac caccgaccag gccaagagca atcagcgccg
    45061 gttgttcgag cgcgataccc gttacgccag cgcccagccc cagcaggata atgccgatat
    45121 tttccagggt gtggtaagcc agcaggcgct ggatgttgtg ttccatcaac gcatacagac
    45181 caccgacgaa cgcggtgatc atgccgagca ccagcagcgc gatcccccac cacagcggtg
    45241 cattaccgcc cagcagtgac agggttaaca tgcccagcag gccaattttc atgactaccg
    45301 tagaaaacaa cgcggcagct ggcgcagagg cgttagcatg tgcctgcggc acccagccgt
    45361 gcagcggaat aatcccggcc agcaggccaa agccaatcac accgagcagc cagatatcgg
    45421 aaccgagcgg cagctgttgc atacgcatat ccagtaggca cagatccagc gtgccataac
    45481 gctgccacag cagccagcaa gcaatcacca gcagcagagt gccaagacgc cccagcgcaa
    45541 accacagttt gccctctttg ctgttgctgg tgaggaacac cgcacacaag gccatgactt
    45601 cggccattac cacgaacatg ccgaggttgc tggcgatgac ggcgcagacg gcggcagcca
    45661 tcaacatatt gatctgcaag ccgttgcact tcacctgcgc gtggcgatgc cagtcaatgt
    45721 tgtagaggct gacaaacaga ccgcacagac cgagcgtaat cagccaaatc gcgttaagcg
    45781 gagagatttg cacatcgtag cttaccagcg acagcgcacc gctcacgcca accgcgccag
    45841 tcagtacagt gaagcccgcg gctgccgtat acagactacc aaccgcgccg ccaattccag
    45901 ctatccagcc acttaacgct ttttgaaaag aaaagagaaa tgccagaaca gcggcggcga
    45961 caaaccatgc cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg
    46021 agcctgctga aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg
    46081 tttgctgacg cgggcgatat cggtgttatc caccagatgc agggctttag tcgggcacat
    46141 ccgcacgcag gccggacctt gttcatcaaa actgcaaagg tcacatttga cggcgatcgc
    46201 gcgaataccc ggcacccagt caagcagcgt actgatacgc gccggagcag gcggtgccgg
    46261 tggcgctttc ggggtattgg cgtttgccgg aatatccagc ggacggctgc cggaaaattc
    46321 aattgcgcca aacgggcagg cgatgccgca cagtttgcag cttacgcaca gactttcatt
    46381 caactgcacg gccccatcaa tgcgggtgat ggcgttaacc gggcaaacca ccgcacaggg
    46441 tgcatcttca cagtggtgac agagctgcgg cgcagattct ttttcattca gcatcactct
    46501 caggcgcggc attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca
    46561 agtgtggcag ccgatacaga gcgtggagtc aacaattaca aaacgattca ccaggcattc
    46621 ctcaggtgat tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat
    46681 cgacacgccc atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg
    46741 ctgccgggct ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatag
    46801 cggtgtataa gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt
    46861 cgcgatcgcg gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg
    46921 caatccgctg tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt
    46981 taaagccatc cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt
    47041 ctttgtccgg cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct
    47101 gaaccagcga attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga
    47161 tgtaatcggc tttctcgctt atttcccaaa tagtcattgt caggttaccc gtttaacaga
    47221 gatgccagct ttaagcattt tctatgccaa cttttaattt attgttatta aatagatttt
    47281 taagctaaag atgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat
    47341 tcaccgcagg gataatcaac actggcacaa ttattgcttg tagctggcaa tagttaatgg
    47401 gaggcgatat gcacgaaata accctctgcc aacgggcact ggaattgatc gaacagcagg
    47461 ccgcaaaaca cggcgcaaaa cgcgtaactg gggtctggct caaaattggc gcattttctt
    47521 gtgtcgaaac cagctctctt gccttttgtt ttgatctggt ttgccgcggc agcgtggcgg
    47581 aaggttgtaa actgcacctc gaagaacaag aggccgaatg ctggtgtgaa acatgccaac
    47641 agtatgtgac gctactgacc cagcgcgtcc gccgctgtcc acagtgtcat ggtgacatgc
    47701 tacagattgt ggcagacgac ggtttacaga ttcggcggat agaaatagac caggagtgag
    47761 cgatgtgtac aacatgcggt tgcggtgaag gcaacctgta tatcgagggt gatgaacata
    47821 accctcattc cgcgtttcgt agcgcgccat ttgccccggc ggcacgcccg aagatgaaaa
    47881 tcaccggcat taaagcgcct gaatttaccc ccagccagac tgaagaaggc gacctgcatt
    47941 acggtcatgg cgaagcgggc actcacgcgc cgggcatgag ccagcgtcgg atgctggaag
    48001 tcgaaattga cgtgctggac aaaaataacc gtctggctga acgcaaccgc gcgcgctttg
    48061 ctgcccgcaa gcaactggtg ctcaacctgg tttccagccc tggttccggt aaaaccaccc
    48121 tgctgacgga aaccctaatg cgcctgaaag acagcgttcc gtgcgcggtt attgaaggtg
    48181 accagcaaac cgtgaacgat gccgcacgca ttcgcgctac cggcacacca gcgattcagg
    48241 tgaacaccgg taaaggctgc catcttgacg cacagatgat tgccgacgcc gcaccgcgtc
    48301 tgccactgga cgataacggt attctgttta tcgaaaacgt tggcaacctc gtatgcccgg
    48361 ccagcttcga tctcggtgaa aaacacaaag tggcggtgct ttccgttacc gaaggtgaag
    48421 acaaaccgct gaaatatccg catatgtttg ccgccgcctc gctgatgctg ctcaacaaag
    48481 ttgacctgtt gccgtatctc aactttgacg ttgagaagtg catcgcctgc gcccgcgaag
    48541 tcaatccaga aattgaaatc atccttattt ccgccaccag cggcgaaggg atggaccagt
    48601 ggctgaactg gctggagaca cagcgatgtg cataggcgtt cccggccaga tccgcaccat
    48661 tgacggcaac caggcaaaag tcgacgtctg cggcattcag cgcgatgtcg atttaacgtt
    48721 agtcggcagc tgcgatgaaa acggtcagcc gcgcgtgggc cagtgggtac tggtacacgt
    48781 tggctttgcc atgagcgtaa ttaatgaagc cgaagcacgc gacactctcg acgccttaca
    48841 aaacatgttt gacgttgagc cggatgtcgg cgcgctgttg tatggcgagg aaaaataatg
    48901 cgttttgttg atgaatatcg cgcgccggaa caggtgatgc agttaattga gcatctgcgc
    48961 gaacgtgctt cacatctctc ttacaccgcc gaacgccctc tgcggattat ggaagtgtgc
    49021 ggtggtcata cccacgccat ttttaaattc ggcctcgacc agttactgcc tgaaaacgtt
    49081 gagtttatcc acggtccggg ttgcccggtg tgcgtactgc cgatgggtag aatcgacacc
    49141 tgcgtggaga ttgccagcca tccggaagtc atcttctgta cctttggcga cgccatgcgc
    49201 gtgccgggga aacagggatc gctgttgcag gcaaaagcac gcggtgccga tgtgcgcatc
    49261 gtttactcgc cgatggatgc gttgaaactg gcgcaggaga atccaacccg taaagtcgta
    49321 ttcttcggct taggttttga aaccaccatg ccgaccaccg ccatcaccct gcaacaggcg
    49381 aaagcccgcg atgtgcagaa tttttacttc ttctgccagc atattacgct tatcccgacg
    49441 ctgcgcagtt tgctggaaca gccggataac ggtatcgacg cgttcctcgc gccgggccac
    49501 gttagtatgg ttatcggcac tgatgcctat aattttatcg ccagcgattt tcatcgtccg
    49561 ctggtggtcg ctggtttcga accccttgat ctactgcaag gcgtggtcat gctggtggag
    49621 cagaaaatag cggcccacag caaggtagag aatcagtatc gtcgggtggt accggatgcc
    49681 ggtaacctgc tggcgcaaca ggcaattgct gatgtgttct gtgtcaacgg cgacagcgaa
    49741 tggcgcggct taggcgtgat tgaatcttct ggcgtgcacc tgacgccgga ttatcaacga
    49801 ttcgatgccg aagcacattt ccgcccagca ccgcagcagg tctgcgatga cccgcgcgcg
    49861 cgttgtggtg aggtattaac gggcaaatgt aagccgcatc aatgcccgct gtttggtaac
    49921 acctgtaatc ctcaaaccgc atttggtgca ctgatggtct cctccgaagg agcgtgcgcc
    49981 gcgtggtatc agtatcgtca gcaggagaat gaagcgtgaa taatatccaa ctcgcccacg
    50041 gcagcggcgg ccaggcgatg cagcaattaa tcaacagcct gtttatggaa gcctttgcca
    50101 acccgtggct ggcagagcag gaagaccagg cacgtcttga tctggcgcag ctggtagcag
    50161 aaggcgaccg tctggcgttc tccaccgaca gttacgtcat tgacccgctg ttctttcctg
    50221 gcggtaatat cggcaagctg gcgatttgcg gcacagccaa tgacgttgcg gtcagtggcg
    50281 ctattccgcg ctatctctcc tgtggcttta tcctcgaaga aggattgccg atggagacac
    50341 tgaaagccgt agtgaccagc atggcagaaa ccgcgcgcgc ggcaggcatt gccatcgtta
    50401 ctggcgatac taaagtggtg cagcgcggcg cggcagataa actgtttatc aacaccgctg
    50461 gcatgggcgc aattccggcg aatattcact ggggcgcaca gacgctaacc gcaggcgatg
    50521 tattactggt tagcggcacg ctcggggacc acggggcgac tatccttaac ctgcgtgagc
    50581 agctggggct ggatggcgaa ctggtcagcg actgcgcggt actgacgccg cttattcaga
    50641 cgctgcgtga cattcccggc gtgaaagcgc tgcgtgatgc cacccgtggt ggtgtgaacg
    50701 cggtagttca tgagttcgcg gcagcctgcg gttgtggtat tgaaatttca gaagtggcac
    50761 tgccggttaa acctgccgtg cgcggcgttt gcgaactgct gggactggac gcccttaact
    50821 ttgccaacga aggcaaactg gtgatcgccg ttgaacgcaa cgcggcagag caagtgctgg
    50881 cagcgttaca ttcccatcca ctggggaaag acgcggcgct aattggtgaa gtggtggaac
    50941 gtaaaggtgt tcgtcttgcc ggtctgtatg gtgtgaaacg aaccctcgat ttaccacacg
    51001 ccgaacctct tccgcgtata tgctaataaa attctaaatc tcctatagtt agtcaatgac
    51061 cttttgcacc gctttgcggt gctttcctgg aagaacaaaa tgtcatatac accgatgagt
    51121 gatctcggac agcaagggtt gttcgacatc actcggacac tattgcagca gcccgatctg
    51181 gcctcgctgt gtgaggctct ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg
    51241 gctattgtgt tgtggcaagc gcagactcaa cgtgcgtctt attacgcatc gcgtgaaaaa
    51301 gacaccccat taaatatgaa gacgaaactg ttctggcaca cggtccggta cgccgcattt
    51361 tgtcgcgccc tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc tggccgcagc
    51421 tggtcgcagg tgggctatac cccaaatttg gtcactattg cctgatgcca ctggcggcgg
    51481 aagggcatat ttttggtggc tgtgaattta ttcgttatga cgatcgtccc tggagcgaaa
    51541 aagagttcaa tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc gaacaaatcc
    51601 agagccgcgt cgtcaacaat gtcgactatg agttgttatg ccgggagcgc gataacttcc
    51661 gcatcctggt tgccattacc aacgcggtgc tttcccgcct ggatatggac gaactggtca
    51721 gcgaagtcgc caaagaaatc cattactatt tcgacattga cgatatcagt atcgtcttac
    51781 gcagccaccg taaaaacaaa ctcaacatct actccactca ctatcttgat aaacaacatc
    51841 ccgcccacga acagagcgaa gtcgatgaag ccggaaccct caccgaacgc gtgttcaaaa
    51901 gtaaagagat gctgctgatc aatctccacg agcgggacga tttagccccc tatgaacgca
    51961 tgttgttcga cacctagggc aaccagattc aaaccttgtg cctgttaccg ctgatgtctg
    52021 gcgacaccat gctgggcgtg ctgaaactgg cgcaatgcga agagaaagtg tttaccacta
    52081 ccaatctgaa tttgctgcgc cagattgccg aacgtgtggc aatcgctgtc gataacgccc
    52141 tcgcctatca ggaaatccat cgtctgaaag aacggctggt tgatgaaaac ctcgccctga
    52201 ccgagcagct caacaatgtt gatagtgaat ttggcgagat tattggccgc agcgaagcca
    52261 tgtacagcgt gcttaaacaa gttgaaatgg tggcgcaaag tgacagtacc gtgctgatcc
    52321 tcggtgaaac tggcacgggt aaagagctga ttgcccgcgc gatccataat ctcagtgggc
    52381 gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga ttgctggaaa
    52441 gcgatctgtt tggtcatgag cgtggggctt ttaccggtgc cagcgcccag cgcattggtc
    52501 gttttgaact ggcggataaa agctctctgt tcctcgacga agtgggcgat atgccactgg
    52561 agttacagcc gaagttgctg cgtgtattgc aggaacagga gtttgaacgt ctcggcagca
    52621 acaaaatcat tcagacggac gtacgtttaa ttgccgcgac taaccgcgat ctgaaaaaaa
    52681 tggtcgccga ccgagagttc catagcgatc tctattaccg cctgaacgta ttcccgattc
    52741 acctgccgcc actacgcgag cgtccggaag atattccgct gctggcgaaa gcctttacct
    52801 tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag acgctgcgca
    52861 ccttgagcaa catggagtgg ccgggtaacg tacgcgaact ggaaaacgtc attgagcgcg
    52921 cggtattgct aacacgcggt aacgtgctgc agctgtcatt gccagatatt gttttaccgg
    52981 aacctgaaac gccgcctgcc gcaacggttg tcgcccagga gggcgaagat gaatatcagt
    53041 tgattgtgcg cgtgctgaaa gaaaccaacg gcgtggttgc cgggcctaaa ggcgctgcgc
    53101 aacgtctggg gctgaaacgc acgaccctgc tgtcacggat gaagcggctg ggaattgata
    53161 aatcggcatt gatttaactg caaattgccg gacagatctg cctgtccggc atactattca
    53221 cgaggttttt tcggacgata tttttccggc agttctggta ccggacgctt gtcatcgatg
    53281 agatgacgca cggttaagat cggatgacgc cacagcattc tcggcccggc ccaacgcata
    53341 atctgtttca tctcttcacg ctttgcaggc tggtaacagt gcaccggaca ctgcttacag
    53401 gctggtttct cttcgccgaa cacacattta tccagccgct tttgcgcgta aacaaacaac
    53461 gcctcgtaat gctccggctc cgctgacgcc tgcgggcatt tcgcttgata aagatcgatc
    53521 atttttttaa tcgtcagttt ttcacgagag atacgcttgc cggacatgct gcctccatac
    53581 tcttaaggtg tatttatatt acaacttaat ttgaaagggg actatggctc tgaaggagga
    53641 ggtttattca accgacgcaa ttttgcgtac ttgcttcata agcatcacgc aaaagctgca
    53701 aaacagcatc tttcccggaa ccagcatcaa gaactcgccg ttcgcttctt cccctgaaat
    53761 gattaactcc ggtatcatgt gcgccttatg tgattacaac gaaaataaaa accatcacac
    53821 cccatttaat atcagggaac cggacataac cccatgagtg caatagaaaa tttcgacgcc
    53881 catacgccca tgatgcagca gtatctcagg ctgaaagccc agcatcccga gatcctgctg
    53941 ttttaccgga tgggtgattt ttatgaactg ttttatgacg acgcaaaacg cgcgtcgcaa
    54001 ctgctggata tttcactgac caaacgcggt gcttcggcgg gagagccgat cccgatggcg
    54061 gggattccct accatgcggt ggaaaactat ctcgccaaac tggtgaatca gggagagtcc
    54121 gttgccatct gcgaacaaat tggcgatccg gcgaccagca aaggtccggt tgagcgcaaa
    54181 gttgtgcgta tcgttacgcc aggcaccatc agcgatgaag ccctgttaca ggagcgtcag
    54241 gacaacctgc tggcggctat ctggcaggac agcaaaggtt tcggctacgc gacgctggat
    54301 atcagctccg ggcgttttcg cctgagcgaa ccggccgacc gcgaaacgat ggcggcagag
    54361 ctgcaacgca ctaatccggc ggaactgctg tatgcagaag atttcgccga gatgtcgctg
    54421 attgaaggcc gtcgcggcct gcgccgtcgc ccgctgtggg agtttgaaat cgacaccgcg
    54481 cgccagcagt tgaatctgca atttggcacc cgcgatctgg tcggttttgg cgtcgagaac
    54541 gcgccgcgcg gactttgtgc tgccggttgt ctgttgcagt atgcgaaaga tacccaacgc
    54601 acgaccctgc cgcatattcg ttctatcacc atggaacgtg agcaggacag catcattatg
    54661 gatgccgcga cacgtcgtaa cctggaaatt actcagaacc tggcgggcgg tgcggaaaat
    54721 acgctggctt ctgtgctcga ctgcaccgtc acgccgatgg gtagtcgtat gctgaaacgc
    54781 tggctgcata tgccagtgcg cgatacccgc gtgttgcttg agcgccagca aactattggc
    54841 gcattgcagg atttcaccgc cgagttgcag ccggtactgc gtcaggtcgg cgacctggaa
    54901 cgtattctgg cacgtctggc tttacgaact gctcgcccac gcgatctggc ccgtatgcgc
    54961 cacgctttcc agcaactgcc ggagctgcgt gcgcagttag aaactgtcga tagtgcaccg
    55021 gtacaggcgc tacgtgagaa gatgggcgag tttgccgagc tgcgcgatct gctggagcga
    55081 gcaatcatcg acacaccgcc ggtgctggta cgcgacggtg gtgttatcgc atcgggctat
    55141 aacgaagagc tggatgagtg gcgcgcgctg gctgacggcg cgaccgatta tctggagcgt
    55201 ctggaagtcc gcgagcgtga acgtaccggc ctggacacgc tgaaagttgg ctttaatgcg
    55261 gtgcacggct actacattca aatcagccgt gggcaaagcc atctggcacc catcaactac
    55321 atgcgtcgcc agacgctgaa aaacgccgag cgctacatca ttccagagct aaaagagtac
    55381 gaagataaag ttctcacctc aaaaggcaaa gcactggcac tggaaaaaca gctttatgaa
    55441 gagttgttcg acctgctgtt gccgcatctg gaagcgttgc aacagagcgc gagcgcgctg
    55501 gcggaactcg acgtgctggt gaacctggcg gaacgggcct ataccctgaa ctacacctgc
    55561 ccgaccttca ttgataaacc gggcattcgc attaccgaag gtcgccatcc ggtagttgaa
    55621 caagtactga atgagccatt tatcgctaac ccgctgaatc tgtcgccgca gcgccgtatg
    55681 ttgatcatca ccggtccgaa catgggcggt aaaagtacct atatgcgcca gaccgcactg
    55741 attgcgctga tggcctacat cggcagctat gtaccggcac aaaaagtcga gattggacct
    55801 atcgatcgca tctttacccg cgtaggcgcg gcagatgacc tggcttccgg gcgctcaacc
    55861 tttatggtgg agatgactga aaccgccaat attttacata acgccaccga atacagtctg
    55921 gtgttaatgg atgagatcgg gcgtggaacg tccacctacg atggtctgtc gctggcgtgg
    55981 gcgtgcgcag aaaatctggc gaataagatt aaggcattga cgctgtttgc cacccactat
    56041 ttcgagctga cccagctacc ggagaaaatg gaaggcgtcg ccaacgtaca tctcgatgcg
    56101 ctggagcacg gcgacactat cgcctttatg cacagcgtgc aggacggcgc ggcgagcaaa
    56161 agctacggtc tggcggttgc tgctctggcg ggcgtaccga aagaggttat taagcgcgca
    56221 cggcaaaaac tgcgtgagct ggaaagcatt tcgccgaacg ccgccgcgac gcaagtggat
    56281 ggtacgcaga tgtctttgct gtcagtgcca gaagaaactt cgcctgcggt cgaagcactg
    56341 gaaaatctcg acccggattc actgactccg cgtcaggcgc tggagtggat ttatcgcttg
    56401 aagagtctgg tgtaataaca attcccgata gtcttttgct atcgggaata ttaacgacaa
    56461 ctgacgaatc aaataaaaat accctgtata ataggaaagc ttattttaca gggtaaaacc
    56521 atgccatcta cacgctatca aaaaatcaat gcccatcact atcgccatat atgggtcgtt
    56581 ggtgatattc atggtgaata tcagttatta caatcccgtt aacatcaact ctctttttac
    56641 cctgaaaccg acttacttat ttccacaggc gataatattg atcgtgggcc gaagagtctt
    56701 aacgtgctgc gcctgctaaa ccaaccctgg tttacgtcgg ttaaaggcaa ccacgaagcg
    56761 atggcgttgg atgcattcga aactggcgat ggaaatatgt ggcttgccag cggtggtgac
    56821 tggtttttcg atttaaatga ttcagagcaa caagaggcaa tagatctgtt gctgaaattc
    56881 catcaccttc cacatattat tgaaatcatt aatgacaaca taaaatatgc catc
//
LOCUS       DAZRVY010000013        55730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZRVY010000013 DAZRVY010000000
VERSION     DAZRVY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.013"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHXOX_000790"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHXOX_000790"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR7004350.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXOX_000791"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXOX_000791"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR7004351.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXOX_000792"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXOX_000792"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR7004352.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXOX_000793"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXOX_000793"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR7004353.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXOX_000794"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXOX_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR7004354.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXOX_000795"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXOX_000795"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR7004355.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXOX_000796"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXOX_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR7004356.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXOX_000797"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXOX_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR7004357.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHXOX_000798"
     CDS             6537..6773
                     /locus_tag="ACHXOX_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR7004358.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHXOX_000799"
     CDS             6776..7633
                     /locus_tag="ACHXOX_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR7004359.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHXOX_000800"
     CDS             7654..9645
                     /locus_tag="ACHXOX_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR7004360.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXOX_000801"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXOX_000801"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR7004361.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHXOX_000802"
     CDS             complement(10248..11144)
                     /locus_tag="ACHXOX_000802"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR7004362.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHXOX_000803"
     CDS             complement(11193..11432)
                     /locus_tag="ACHXOX_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR7004363.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXOX_000804"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXOX_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR7004364.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXOX_000805"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXOX_000805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR7004365.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXOX_000806"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXOX_000806"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR7004366.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXOX_000807"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXOX_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR7004367.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHXOX_000808"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHXOX_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXOX_000809"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXOX_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR7004368.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXOX_000810"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXOX_000810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR7004369.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXOX_000811"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXOX_000811"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR7004370.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHXOX_000812"
     CDS             complement(21570..22739)
                     /locus_tag="ACHXOX_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7004371.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXOX_000813"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXOX_000813"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR7004372.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHXOX_000814"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHXOX_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR7004373.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXOX_000815"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXOX_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR7004374.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHXOX_000816"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHXOX_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR7004375.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXOX_000817"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXOX_000817"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR7004376.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXOX_000818"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXOX_000818"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR7004377.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXOX_000819"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXOX_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR7004378.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHXOX_000820"
     CDS             complement(30320..31576)
                     /locus_tag="ACHXOX_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR7004379.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHXOX_000821"
     tRNA            31884..31960
                     /locus_tag="ACHXOX_000821"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHXOX_000822"
     tRNA            31965..32041
                     /locus_tag="ACHXOX_000822"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHXOX_000823"
     CDS             32149..32454
                     /locus_tag="ACHXOX_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR7004380.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHXOX_000824"
     CDS             32580..34184
                     /locus_tag="ACHXOX_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR7004381.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXOX_000825"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXOX_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR7004382.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXOX_000826"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXOX_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR7004383.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHXOX_000827"
     CDS             complement(35794..36462)
                     /locus_tag="ACHXOX_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR7004384.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHXOX_000828"
     CDS             complement(36526..37164)
                     /locus_tag="ACHXOX_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR7004385.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHXOX_000829"
     CDS             complement(37177..39279)
                     /locus_tag="ACHXOX_000829"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR7004386.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHXOX_000830"
     CDS             complement(39300..39926)
                     /locus_tag="ACHXOX_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR7004387.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="ACHXOX_000831"
     CDS             complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="ACHXOX_000831"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR7004388.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            40946..41140
                     /locus_tag="ACHXOX_000832"
     CDS             40946..41140
                     /locus_tag="ACHXOX_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004389.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            41148..42560
                     /gene="pykF"
                     /locus_tag="ACHXOX_000833"
     CDS             41148..42560
                     /gene="pykF"
                     /locus_tag="ACHXOX_000833"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR7004390.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42870..43106
                     /gene="lpp"
                     /locus_tag="ACHXOX_000834"
     CDS             42870..43106
                     /gene="lpp"
                     /locus_tag="ACHXOX_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR7004391.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="ACHXOX_000835"
     CDS             complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="ACHXOX_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR7004392.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="ACHXOX_000836"
     CDS             complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="ACHXOX_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR7004393.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="ACHXOX_000837"
     CDS             complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="ACHXOX_000837"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR7004394.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="ACHXOX_000838"
     CDS             complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="ACHXOX_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR7004395.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="ACHXOX_000839"
     CDS             complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="ACHXOX_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR7004396.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="ACHXOX_000840"
     CDS             complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="ACHXOX_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR7004397.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="ACHXOX_000841"
     CDS             complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="ACHXOX_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR7004398.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50382..50570)
                     /locus_tag="ACHXOX_000842"
     CDS             complement(50382..50570)
                     /locus_tag="ACHXOX_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR7004399.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50670..51080)
                     /gene="menI"
                     /locus_tag="ACHXOX_000843"
     CDS             complement(50670..51080)
                     /gene="menI"
                     /locus_tag="ACHXOX_000843"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR7004400.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51077..54133)
                     /locus_tag="ACHXOX_000844"
     CDS             complement(51077..54133)
                     /locus_tag="ACHXOX_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR7004401.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54522..55634
                     /gene="ydiK"
                     /locus_tag="ACHXOX_000845"
     CDS             54522..55634
                     /gene="ydiK"
                     /locus_tag="ACHXOX_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR7004402.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13414 a        14653 c        13645 g        14018 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca
    40141 attaaaatta caaagcaaga aattataagt gaactgatat ttattatcaa ttgaaataaa
    40201 tttaacttaa gcgacaatta tttgtgacgt tcattgcaga aaataaaaat aatattatcg
    40261 ttatagatta aaatacatac cgcaattcgt ataatttcag cagttgttat ttagaatgat
    40321 taactggacc gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg
    40381 gtcattttac cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct
    40441 gaatgcgttt attggctaat gcagtacgta atacgcctgc gataacaata tgctttggtt
    40501 cctgccccag atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt
    40561 acagttcatc ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaactat
    40621 cagcatatat caatctaacc aattaaacaa aaatcaaaca aaatcagaca aataacgcga
    40681 taaattattt taactgtagc aattgagcga tgatatattt atacaccgga tgaactttca
    40741 cttatcctca cactgacaac ttcggcacca gacgttgcgc aaacagcgaa gtttttgcgt
    40801 aaccttttcc ctggaacgtt aaatctttga taacaattta ttgtctaaca agttgtatat
    40861 tttttgaaac gctgtttttg ttttcctttt ggattaattt cagcgtataa tgcgcgccaa
    40921 ttgactcttg aatggtttca gcactttgga ctgtagaact caacgactca aaaacaggca
    40981 ctcacgttgg gctgagacac aagcacacat tcctctgcac gctttttcga tgtcacctat
    41041 ccttagagca aggcaccacc actttcgtaa taccggattc gctttccggc agtgcgccca
    41101 gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcatg aaaaagacca
    41161 aaattgtttg caccatcgga ccgaaaaccg aatctgaaga gatgttagct aaaatgctgg
    41221 acgctggcat gaacgttatg cgtctgaact tctctcatgg tgactatgca gaacacggtc
    41281 agcgcattca gaatctgcgc aacgtgatga gcaaaactgg taaaaccgcc gctatcctgc
    41341 ttgataccaa aggtccggaa atccgcacca tgaaactgga aggcggtaac gacgtttctc
    41401 tgaaagcggg tcagaccttt actttcacca ctgataaatc tgttatcggc aacagcgaaa
    41461 tggttgcggt aacgtatgaa ggtttcacta ctgacctgtc tgttggcaac accgtactgg
    41521 ttgacgatgg tctgatcggt atggaagtta ctgccattga aggtaacaaa gttatctgta
    41581 aagtgctgaa caacggcgac ctgggcgaaa acaaaggtgt gaacctgcct ggcgtttcca
    41641 ttgccctgcc agcactggct gaaaaagaca aacaggacct gatctttggt tgcgaacaag
    41701 gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc gaaatccgtg
    41761 agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc gaaaaccagg
    41821 aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg gttgcgcgtg
    41881 gcgacctggg tgtagaaatt ccggtagaag aagttatctt cgcccagaag atgatgatcg
    41941 aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg gattccatga
    42001 tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc atcctcgacg
    42061 gtactgacgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg ctggaagcgg
    42121 tttctatcat ggcgaccatc tgcgaacgta ccgaccgcgt gatgaacagc cgtctcgagt
    42181 tcaacaatga caaccgtaaa ctgcgcatta ccgaagcggt atgccgtggt gccgttgaaa
    42241 ctgctgaaaa actggatgct ccgctgatcg tggttgctac ccagggcggt aaatctgctc
    42301 gcgcagtacg taaatacttc ccggatgcca ccatactggc actgaccacc aacgaaaaaa
    42361 cggctcatca gttggtactg agcaaaggcg ttgtgccgca gctggttaaa gagatcactt
    42421 ctactgatga tttctaccgt ctgggtaaag aactggctct gcagagcggt ctggcacaca
    42481 aaggtgacgt cgtagttatg gtttctggtg cactggtacc gagcggcact actaacaccg
    42541 catctgttca cgtcctgtaa tattgctttt gtgaattaat ttgtatatcg aagcgccctg
    42601 atgggcgctt ttttatttaa tcgataacca gaagcaataa aaaatcaaat cggatttcac
    42661 tatataatct cactttatct aagatgaatc cgatggaagc atcctgtttt ctctcagttt
    42721 ttttatctaa aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa taagtgcctt
    42781 cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa cgctacatgg
    42841 agattaactc aatctagagg gtattaataa tgaaagctac taaactggta ctgggcgcgg
    42901 taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagctgt
    42961 cttctgacgt tcagactctg aacgctaaag ttgaccagct gagcaacgac gtgaacgcaa
    43021 tgcgttccga cgttcaggct gctaaagatg acgcagctcg tgctaaccag cgtctggaca
    43081 acatggctac taaataccgc aagtaatagt acctgtgaag tgaaaaatgg cgcacattgt
    43141 gcgccatttt tttgtctgct atttaccact actgcgtcgc gcgtaacata ttcccttgct
    43201 ctggttcacc attctgcgct gactctactg aaggcgcatt gctggctgcg ggagttgctc
    43261 cactgctcac cgaaaccgga taccctgccc gacgatacaa cgctttatcg actaacttct
    43321 gatctacagc cttattgtct ttaaattgcg taaagcctgc tggcagtgtg tatggcattg
    43381 tctgaacgtt ttgctgttct tctgctgata gtggtcgatg tacttcaaca taacgcatcc
    43441 cgttaggttc cacggaatat ttcaccggtt cgttgatcac tttcaccggc gttcccgtcc
    43501 gcacgctgga gaacaaggct ttaatatccg gtgcattcat gcgaatacac cctgaactga
    43561 cgcgcaaacc aacgctgtcc ggcgcactgg tgccatgaat gaggtattcg ccattaccat
    43621 gcgcgaggcg cagtgcgtaa cgtcctagcg ggttatttgg tccggcagga acgactggcg
    43681 gtaatttaat gccacgctcc agcgaacgct gacgaatgcc tgccgtaggc gtccaggttg
    43741 ggttagggat tttctgccca acacgcgttt ccatcaccgg cgtttccagc ccctgcaatc
    43801 caatacctat tggataaacc tgcacaatat tttctcccgg cggataataa taaaggcgca
    43861 gctctgcaag gttaacgata atcccctgac gcggtgcatc aggtaataac agttgtgaag
    43921 gaatagttat cgtcgtacca ggttttggca ccgggacgat agtgttattg gcttcaagga
    43981 tcaacattgc cgcagtatca aaacgtcggg caatagcctg aaggttttta tccccttctt
    44041 gcaccgtata cgtttgattt tgcccaacca gtcggcttcc ggttggtggt agcggataat
    44101 caaccgccca ggcagcctgg atggcgctaa aagcgccgat aagcgtgagt gtaagcaaag
    44161 acgcgcgttt cattgtaaac ctcctgtatt tgccggagac tcacgctgaa acgtcggatg
    44221 gcgcttatgt tcacctgaaa ccaaaacact cctgtgcagg tcagtgtaaa cattgaccat
    44281 ccggcaatgt gagccaaccg gatgaaagct gtccctttag tttagctaag tgcagcggct
    44341 ttggcgcgaa ttgcgcgaat catcgcttcc agaccttgtg aacgagatgg ggtgagatgt
    44401 tgggtgagtg ccattttttc aaaccacgga cgcacatcga aactgacaat atcctgcggc
    44461 gtcatctgat cgtagagaat aaagactacc gcaataagcc ctttcacaat cgccgcatcg
    44521 ctgtcgccct gcaattcaat aattccgtgc gcattctggc gcatgacaat ccacacctga
    44581 ctctggcagc cctgaatgct attttgtggg cttcggtctt cgtcgcgtaa ttcagtcaga
    44641 cgctggccca gctcaataat gtagagatat ttctcttccc agttggcgca gcgtaaaaaa
    44701 ttacgcagca atttttcttt atccggcaat aaagccatag tgcctccctg ttatcccagc
    44761 aaacggtgaa tacgttgcag gccggtcacc agacgatcca cttcttcatg ggtgttatac
    44821 atggccagcg acgcccgaca catcgcaggg acgttgtaat aggccattaa tggcatagcg
    44881 cagtgatgtc cggtacgcac agcaatgccg taattatcga gaaaactgcc aacatcatag
    44941 gcgtggtgtt taccgagatt aaaagcaata acgccaagcc tgttttgtgg gccatagaga
    45001 gtgagatccg gtacagattc cagctgtgat agcgcataat gcatcagatt ctgttcatac
    45061 tcggctatgt tattaagccc cagcgccgaa acatactcca gcgccgcgcc aagaccaata
    45121 atgcccccgg tattgggtgt accggcttca aaccgccatg gtgctttggt ccaggtagtg
    45181 ccttcactca ggctgacggt ggcgatcata gaaccgcccc cttcccacgg cggcatctcc
    45241 tgcaacaagg cttctttcac ataaagaatg ccaattccgg tggggccata cagtttatgc
    45301 ccggagaaca cgtaaaagtc gcaatccagc gcctgaacat ccaccggatg atgcatcacc
    45361 gcctgagcgc catccaccag cacttttgcg ccatgctggt gcgcaagcgt gattatttcc
    45421 gccagcggat tttctgttcc cagcacgttg gagacatgag taattgccag caggcgagtt
    45481 ttctcatcaa acagcgtagg cagcgtctcc agttgcagcg taccgtcggg gttgagaggg
    45541 atcacacgca gctctgcgcc aacgcgtgcg caaagcatct gccagggaac aatgttagcg
    45601 tggtgctcca tctgactgat gatgatgtta tcgcccgccc gcacgttgct gttgccccaa
    45661 ctattggcga ccagattgat cccttccgtc gtgccgcgga cgaacaccag ctcttccgcc
    45721 gaacgggcat taataaacag cgatgcccgc ttgcgcacgt tctccatttt ctcggtcgcc
    45781 tgggcgctta aggtatgaat accgcgatgc accgccgcgt agccgtgacg ataaaactca
    45841 gcctcggcgt caatcacctg gcttggtttc tgcgcactgg cggcgctgtc gagataagcc
    45901 agcggcaaac cgttcacctc acgagaaagc accggaaagt cggcccgcac tttgtcgacg
    45961 gaaaaagtca tcttgcacct cctggcagcc gttgaccgat tcgggccagc acctgctgtt
    46021 taagcccctc atcacgcagt gcttccgtca gttcggcagc aaaggcgtaa atgatcatct
    46081 gctgggcatc ctgctgattg atcccgcgcg agcgcagata gaacatctgt tcatcatcaa
    46141 tacgccccac cgtcgcgccg tggctgcatt tcacatcatc tgcatagatt tccagctgcg
    46201 gttttgtatc cacttccgcc attttgccca tcagcagatt attgttggtc atctgaccat
    46261 ccgttttgat ggcgtgctgc gcgacgttga tcaaaccgtt aaataccgcg cggcctttgt
    46321 cgctgacgat agttttgtgc aactgtcggc tgttacaaaa acctttattg tgttccagcc
    46381 aggtacgggt atcacacacc tcgtttttca ccggcatcgc caggctattg atccgcagcg
    46441 tgctgttttc gccattgagt tgcgtactgg tgttgtgtcg taacactgcg ccacccagca
    46501 ggaaactatg gctaaatgcg gtggcatcct cagccagcaa caaatcgtta tgagcaaagt
    46561 ggtgactgag cgggttttca aacgccagct tgatatgctg caagtgggcg ttcgctgcga
    46621 cattgatagt gaaccgtgcc ccggtaaaat gacgagcatc attaaggctg acaaaatgtt
    46681 cgatcaccgt tgcttcggca ccttccgcca gatccagatg atgccggtaa tgggcagtgt
    46741 tcacctcttc acctgccacg ccctgggtga tatgcattaa cagcaatggc tttgccggcc
    46801 gttgaccgcg cttcacggcg atatgcgtca cgctttgtgc caggctttcc gtcaaatgca
    46861 gaaacacttc cgcctgaata gcgtcgggta acccctgacg gtcgtcgtta atgctcactt
    46921 catatccgct gccttcaatt gcatcgctca gcgcgggcac gtaacgtcca tcgacaaaca
    46981 ccagccgcac ggcgtctaac gttaacgcta aggcatcacg ctgctgtggg gatatatctc
    47041 ccgcaatgct gacaaactga ctattggtca gcccttccag cggcgtatat ttccagtttt
    47101 catgtttacg tgtcggcagt ccggtacgca gcaattgttg taaatgctgc tgtgcttgcg
    47161 gtgagcgttt tgtcccttca gcttcaaaca agtgatgcca ctgttgcagc gcattactgc
    47221 tgttcggtaa gccagccata accctgctcc tccagttgtt tgaccaacgt gaaatcgccg
    47281 gatttcacaa ttcgtccctg atatagcaca tggacgtaat caggcttgat gtagtcgaga
    47341 atgcgttggt agtgcgtaac aatgatgaat gagcgcttgc cgtcacgcag cgagttcacg
    47401 ccatcggcaa ccacttttaa tgcgtcaata tccagcccgg agtccgattc atcaagaatg
    47461 cataactccg gttccagcac cgccatttgc aaaatatcgt tgcgcttttt ctcgccgccg
    47521 gaaaaaccaa cgtttaccga acgggttaat aaatcttccg gcatcttcag gagagcgatt
    47581 ttctcttcca tcaaatcctg aaaatcaaag cggtcgagcg tttcctggcc gcgatagctg
    47641 cgcaccgcat taagtgccgt ttgcaggaaa aactggttac tgacgccggg aatctccacc
    47701 ggatactgga aggccataaa gatgccttcg cctgcacgat cttccggcga cagcgcaagc
    47761 aaatctttgc ctttgaactc aaccgtgccg cccgtcactt cataatcttc tcgcccggca
    47821 agcgttgccg ataaggtact tttgcccgaa ccgtttggcc ccataatggc gtgaacttcg
    47881 ccgggacgaa cgtcgaggct taatccacgc aggatagctt tatcttccac gctgacgtgt
    47941 aaatctttaa tacttaacat gtttattcct tatccgacgc tgtgttcaag actgatggcg
    48001 aggagttttt gtgcttcaac ggcaaattcc agcggcagtt ctgagaacac gtctttgcag
    48061 aaaccgttaa caatcatcga gatggcgtct tcttcgctaa tcccgcgttg caggcagtaa
    48121 aacagttgat cttcaccaat acgtgatgtc gttgcctcgt gctccagttg tgcgctgtta
    48181 ttgcgacatt cgacatacgg gaaggtatgc gccccacaat tagcgccaat cagcattgag
    48241 tcgcactgag tgaaattgcg cgcattggtt gccgtcggca tgattttcac taagccgcga
    48301 taactgttct gactatgtcc ggcagagatc cctttcgaga taatggtcga tttggtgttt
    48361 ttaccgatgt ggatcatctt ggtgccggta tccgcttgct gatgaccgct ggtcagcgcc
    48421 actgagtaaa actcaccaat ggagttatcg ccgcgcaaaa tgcagctggg atatttccac
    48481 gtaatcgctg acccggtttc tgattgcgtc catgacattt tgctgttttc gccttcgcac
    48541 aaagcacgct tggtgacgaa attgagaata ccgccggtgt tgttatcgcc agggaaccag
    48601 ttttgtaccg tggaatattt cacctcggcg tttttatgga tgatgacttc caccaccgcc
    48661 gcgtgtaact gatagctgtc acgcaccgga gcggaacagc cttcaatgta gctgacgtag
    48721 ctgtcttcgt cggccaccag aatggtgcgc tcaaactgcc cggttttttc cgcgttaatg
    48781 cgaaaatagg tggaaagttc catcgggcag cgcacgcctt taggcacata aataaacgta
    48841 ccgtcagagg ctaccgccgc attaagcgcg gcaaagaagt tgtcatttcc cggcaccaca
    48901 gtgccgagat atttacgcac cagttccgga tgatcgtgga tcgcctcacc aaaggaacag
    48961 aaaataattc cctgctccgc cagtttttcg cgataagtag tggcaaccga aacagagtcg
    49021 aaaatggcat ccaccgccac ctctttgcct tcccgcacgg gaacgcccaa ctgctcaaac
    49081 gccgcctcca cctctttact taaaaaggcg ttcgcgccag tttgctgcac cgcgccaggt
    49141 tcagacgcgc aagtgtcgtc acaattaccg cacgatggtg ctgagtagta actgtaatcc
    49201 tgataattca gcttgtcgta gtgcgctttc aaccagtgcg gttcttccat ctccagccat
    49261 gcgcgatagg cgtttagacg aaactccagc atccactccg gctcattacg cttcgccgaa
    49321 attgcgcgca ccacctcttc gtttatcccc tttgccagct catcggtggc taactgggtg
    49381 aagaatcctt ctttataatt cagcgggccg ccggtccagg ttttgacatc gtcagttgct
    49441 tcagtattac gagacatagt accgcctata ccccaaagct ttcgccacag ccacattcat
    49501 tctgggcttt agggttgtga aatttgaata tctgattaag tccttcacga acgaaatcga
    49561 cttccgtgcc atcaataaac ggcatcgctt gcagcgggac aaacagcttc gcgccgtcgt
    49621 gttcaaacag cagatcgtct ttgtccggct cgctaacact gtcgagcaca tagccaaagc
    49681 ccgcgcagcc cgtttgcttc acgcctaagc gcacgccgac catacccggc tgctttgcca
    49741 ccagctcacg gatgtgtatc gccgctgcgg gtgtcagcgt taagccttgc caggcgaaat
    49801 cttgtgggtt aaaggttcct gaatgcatgt ccatcgattt acctcacttc atcgctttca
    49861 gcgtataaca gcatgctagt gataatgatt atcagttcaa cccagcaaac gcaggggctt
    49921 taccgtaaaa catgcttttt gcctgctttt aataagcata gaccctgatg tgtgggttaa
    49981 caggcacgct aagaattagg tatctcattg ttagataatg gttattatct aaggagcatt
    50041 aaggctgtga tgaaaaagaa aaagttgtat tgaaaatgac tatttaagag ataggtaaaa
    50101 aagtgcagcg ttcagaaata agaaaaccct taagtctgtg cgacacaggc ttaagggttt
    50161 ctaccccatc cggcgcttat ctccggcact ctcagtggct tagctcttga aggggcgata
    50221 agaataatct cataaagcta acccgccgtt ttaacacaaa ctgcgattag tattattttt
    50281 gaacaatatc aggcggtaga taagcagtat taagaaggtc atcgaacctg gacggaggtt
    50341 aatccaggtc gatttggcga acttgcggca ttaagtcagg atcaatgcac gcccagacgc
    50401 caggcaaagt agatttcttc ttttaattca gtagaagaga gagtaagcag gtcagcaaat
    50461 tcaagttcta gttgtttcag acgtttgaga tattgggcag gtgaaagatt gctctggtca
    50521 cggcgtaaaa attcaatggc cagctgggtg ggatcaagtt gagtagacat agcatcctcg
    50581 cttttagaca agacgtgcac agtataccac cgtttactgt gcagataatg accaaaagca
    50641 atatgcgtca cacttttctg gtgacaacgt cacaaaatgg cggtcgtcaa tcgtgacgaa
    50701 cagcacaaac gccctttctc atcgaagatt tcaatctgcc agacctggtg acgcgaaccg
    50761 agatgcaacg gtttacatat gccgcgcacc cgcccttctc gtgccgagcg gacgtgatta
    50821 gcattgattt ccagaccaac cactttttgc tcaccttcgg tacataaata accggcaacg
    50881 gaaccgatac tttcggccag taccacggat gctcctccat gcagcaaccc gaaaggctgc
    50941 tttgtccgcg agtctactgg cattgtcgct tcaagggtgt catcaccaat atgttcaaag
    51001 cgaatatcca ggaaccccac catgtttcct tcacccatag cattcagtgc ttccagggtg
    51061 attttccgtt tccatatcat ttaataatct ccagtaaagc ctgcacagga tggcgtaccc
    51121 ccgtgccttc aacccgtttt acctggctac ggcaggaata tccggtcgcc agacagcggt
    51181 tacgcggcag tcgctgcata gcctgatgcc aggataactc atagatcccg agcgaatttt
    51241 catggttttt cgcttcatgt ccgtaagtcc ctgccatgcc gcagcaaccc acgctgacat
    51301 tttccagttt cgcgccaaaa cgggcaaata tcgcggccca ttgtgctggc gcgcccggca
    51361 aagcggtaac ctcggtacag tgaccaaaga aataccatga ttcaccgctg actgtagcca
    51421 ctggatgtga ctcaagtgcg cttgccagcc attcattcgc aagtaagacg ttaaactcgc
    51481 cacgttcctc gcccagcgcc agtttatatt catcgcgata acaaagtacc agcgccggat
    51541 cgacgcccac cattggcatc ccaagcttag ccatacggtt gaggaaatcc gccgtctttt
    51601 tcgccgtctt cgcaaaacga ttaagaaagc ctttgatatg ctgggctttg ccatttggcg
    51661 aaaatggcag taacacaggc tggaaaccta atttttcaac cagacggaca aaatccgcca
    51721 ccacttgcgc atcgtaatag ctggtaaagg ggtcctgcac caccaacacc gtgcgcgctt
    51781 tctgctctgc attgagagct tcaagctgtt ccagcgtcat gtttgccgag cgatgcccca
    51841 ccatttgttg ttgtagcgag gggaccgaca gcagtggcaa atcaaccatg ccgatatgtt
    51901 tttccgagag tttgcgcacc agcggctggt taatgaagaa gttaaaggtt ttcggcgcgc
    51961 gtgccatcag cggtgcgtag ctctcgaccg tagcgacgag gtggtcgcgc agcgggcgta
    52021 aataacgggt gtgatagagc tgcaggaaac gagagcgaaa ctccggcaca tcaattttga
    52081 tggggcactg ggtcgaacac gctttacagg ccagacagcc cgacatcgcc tctttgactt
    52141 cgtgtgagaa gtcatattcg cctttattcg catgccagct attgcgcgtg cgggcaatta
    52201 acgtccgtaa actgacgccg gattcaggca gttcttgttc cagtttgagt ggatcaacgc
    52261 cgcgatccgc caacaaacgc agccattcac gcaccagcgt tgcgcgccct ttcggtgaat
    52321 gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg actacgggca tcaaagttga
    52381 agcataaacc gttgccgtta cactccatcg caccgcgcca ctgctggcgt accgcaatgg
    52441 ggatctgccg atcgaatgta ccgcgcttca ccgcgtccac tttcatcatc ggcgcatcga
    52501 gaccttctgg cgggcaaatc ttccctgggt tgagtcggtt atgcgggtca aatgccgctt
    52561 tcactttgcg cagttctgca aaaagttcct caccgaaaaa cgccgggctg tattcagcgc
    52621 gaaaaccttt gccgtgctcg ccccacaaca aaccaccgta tttcgcagtc agcgccacca
    52681 cgtcatcaga gatttgcttc atcaaaatct cttgttgcgg atcgcacata tccagtgccg
    52741 gacggacgtg caaaacacct gcgtcgacgt gaccgaacat accgtagctt aagccgtggc
    52801 tgtcgagcag cgcgcgaaat tcagcaatat aatcggccag atgttccggc ggtacgcagg
    52861 tatcctcagc aaacggaatt ggcttagcgg cacctttggc attgccaagc agaccaacgg
    52921 cttttttgcg cattgcatag atacgttcaa cccccgccag ctcgcggcac acctgccagc
    52981 caatcacacc tgcctgttgg ctggcaatca gctcatcaag ccgcacacag agtgcattaa
    53041 ctcgctcgtc aatcagcgcc tcatcatcac cagcaaattc cacaatgttc agcccgagca
    53101 tctctttgtc aggcacatcg gtaatcaact cgctgacgga atgccagaca atatcttccc
    53161 gcgccagatt cagcactttt gagtccaccg tctctaccga aagcgcccgc gcctcaacca
    53221 taaacggcgc gttacgcagc gcggagtcaa aagagtcata tttgacgttc accagacggc
    53281 gcactttagg caagggcgta atatccagcc gcgcttcggt aataaaggcc agcgtccctt
    53341 ctgaacccgt cagaatgcgc gtcaggtcga actcggtcat ctcatcgtta aagacatgac
    53401 gcagatcgta accggtaaga aagcggttaa gtttggggaa gttgtcgatg attaactggc
    53461 gttgcagacg gcaacgttga taaaccgtgt tataaattcg cccgattgtg gtattggttt
    53521 tacccagcgt ttccgccagt tcgacgggta aaggttgcgt atcgagaata tcgcccccca
    53581 acaacaccgc gcgtacgcca agtacgtgat ctgacgtttt gccatagacc agcgatccct
    53641 gaccggatgc atcggtattg atcatcccgc cgagcgttgc ccggttgctg gtcgaaagtt
    53701 ctggtgcaaa aaagtagccg aacggtttca ggtactgatt gagttgatct tttatcaccc
    53761 cggcctcaac gcgcacccag ccctcttcag ggttaatttc gatgatgcgg ttcatatggc
    53821 gggacatatc aacaataatc ccctggttga gcgcctgacc gttagtgccg gtgccgccgc
    53881 cgcggggggt aaagatcagc gatgaatagc gttcctgcgc ggcaagacgg gcgatcagcg
    53941 ccacatctgc ggttgaacgc ggaaatacca ccgcatcggg gagaagttgg taaatactgt
    54001 tgtcggtcga cattgtcaga cgatcggcat aacttgtcgc cgtatcgccg gtaaaacctt
    54061 gttgctccag ctcttgcaaa aaattaagca ccagttgaac gacgccgggt gcctgggaaa
    54121 tctgtggaat cattatattg accctttcct gcggtctgtg atgtaggtcg atacactatt
    54181 ctttcaggcg gctgcaatag cgcactgaaa ggtgatgttt atttactcta tggatttcga
    54241 gttgcaggaa ggcggcaagc gagtgaactc caggagctta caatagtaag tgactggggt
    54301 gaacgaacgt agccgcagcg catgcaactt gaaatacgac gagtaaatcg tttgcgtgtt
    54361 gcctgagttg ttgtaccaca tttttttcta acacgcccat cagaattaag ggcagaatcg
    54421 gcctgttaaa aaccgctgaa attgctcatc attatgcagg tgagtttcgc gtgttcacgt
    54481 cgcgtcgacg atttgacgca caaaaaaggt gaaaagtagt tatggtaaat gttcgtcagc
    54541 ccagggatgt cgcacaaatt ctgctttcgg tgctgttttt agccatcatg attgtggcat
    54601 gtctgtggat tgttcaaccc tttattctcg gctttgcatg ggccggtacg gtggttatcg
    54661 ccacctggcc ggtattgtta cgtttgcaaa agatcatgtt tggccgccgc tctctcgccg
    54721 ttctggtgat gacgctgtta ttagtgatgg tgtttatcat ccctattgct ttgctggtta
    54781 acagtatcgt cgacggcagc ggcccgctaa ttaaagccat ttccagcggc gacatgacgt
    54841 tacccgatct ggcgtggctt aataccattc cggtgattgg cgcgaagctg tatgcaggct
    54901 ggcacaactt gctggatatg gggggtacgg cgatcatggc gaaagtccgc ccttatattg
    54961 gcaccaccac cacctggttc gttgggcagg cggcgcatat cgggcgcttt atggtgcatt
    55021 gtgcgctgat gcttctcttc agtgccctgc tgtactggcg cggtgaacag gtggcacaag
    55081 gcattcgcca ttttgcaacc cgtctggcag gcgttcgcgg agatgccgcc gtgctgctgg
    55141 cggcacaggc tatccgcgcg gtggcgctgg gtgtggtggt gacggcgtta gtacaggcag
    55201 tgcttggcag tatcggcctc gccgtatccg gcgtacctta tgcaacgttg ctaacggtgt
    55261 taatgatcct ctcctgcctt gtccagcttg gcccgttgcc ggtactgatt ccggcgatta
    55321 tctggctcta ctggaccggc gataccacct ggggaacggt attgttagtg tggagcggtg
    55381 tggttggcac gctggataac gtcatccgcc caatgttaat tcgcatgggt gccgatttac
    55441 cgctgatcct gatcctctct ggcgttattg gtggtttgat tgctttcggg atgatcggtc
    55501 tgtttattgg tccggttctg ttagccgttt cctggcgtct gtttgccgcg tgggtggaag
    55561 aagtcccgcc gccgacggac caaccggaag aaattctcga agaacttggc gaaatcgaga
    55621 aaccgaataa gtaatttctc atcaggcggc tctgccgcct gattgttaat caccgctaat
    55681 tgatatttgc ttgctcttcc ccatattttc ctgcttaccg ccattcagct
//
LOCUS       DAZRVY010000014        54950 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZRVY010000014 DAZRVY010000000
VERSION     DAZRVY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.014"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHXOX_000846"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHXOX_000846"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR7004403.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHXOX_000847"
     CDS             complement(1672..2667)
                     /locus_tag="ACHXOX_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7004404.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHXOX_000848"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHXOX_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHXOX_000849"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHXOX_000849"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR7004405.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHXOX_000850"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHXOX_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR7004406.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHXOX_000851"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHXOX_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR7004407.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHXOX_000852"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHXOX_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR7004408.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHXOX_000853"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHXOX_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR7004409.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHXOX_000854"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHXOX_000854"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR7004410.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHXOX_000855"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHXOX_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR7004411.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHXOX_000856"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHXOX_000856"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR7004412.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHXOX_000857"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHXOX_000857"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR7004413.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHXOX_000858"
     CDS             complement(17699..18322)
                     /locus_tag="ACHXOX_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR7004414.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHXOX_000859"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHXOX_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR7004415.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHXOX_000860"
     CDS             complement(18971..19213)
                     /locus_tag="ACHXOX_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR7004416.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHXOX_000861"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHXOX_000861"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR7004417.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHXOX_000862"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHXOX_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR7004418.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHXOX_000863"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHXOX_000863"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR7004419.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHXOX_000864"
     CDS             22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHXOX_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR7004420.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHXOX_000865"
     CDS             24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHXOX_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR7004421.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25052..25909
                     /locus_tag="ACHXOX_000866"
     CDS             25052..25909
                     /locus_tag="ACHXOX_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7004422.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_000867"
                     /pseudo
     CDS             complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHXOX_000868"
     CDS             complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHXOX_000868"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR7004423.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHXOX_000869"
                     /pseudo
     CDS             complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHXOX_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30569..31816)
                     /locus_tag="ACHXOX_000870"
     CDS             complement(30569..31816)
                     /locus_tag="ACHXOX_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR7004424.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31838..32164)
                     /locus_tag="ACHXOX_000871"
     CDS             complement(31838..32164)
                     /locus_tag="ACHXOX_000871"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR7004425.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHXOX_000872"
     CDS             complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHXOX_000872"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR7004426.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            33553..34809
                     /locus_tag="ACHXOX_000873"
     CDS             33553..34809
                     /locus_tag="ACHXOX_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR7004427.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHXOX_000874"
     CDS             34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHXOX_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR7004428.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35391..36629)
                     /locus_tag="ACHXOX_000875"
     CDS             complement(35391..36629)
                     /locus_tag="ACHXOX_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR7004429.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36965..38167
                     /gene="nupC"
                     /locus_tag="ACHXOX_000876"
     CDS             36965..38167
                     /gene="nupC"
                     /locus_tag="ACHXOX_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR7004430.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38217..40405)
                     /locus_tag="ACHXOX_000877"
                     /pseudo
     CDS             complement(38217..40405)
                     /locus_tag="ACHXOX_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(40614..40689)
                     /locus_tag="ACHXOX_000878"
     tRNA            complement(40614..40689)
                     /locus_tag="ACHXOX_000878"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40654..40656),aa:Ala,seq:ggc)
     gene            complement(40729..40804)
                     /locus_tag="ACHXOX_000879"
     tRNA            complement(40729..40804)
                     /locus_tag="ACHXOX_000879"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40769..40771),aa:Ala,seq:ggc)
     gene            41025..41384
                     /locus_tag="ACHXOX_000880"
     CDS             41025..41384
                     /locus_tag="ACHXOX_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR7004431.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41386..41778
                     /locus_tag="ACHXOX_000881"
     CDS             41386..41778
                     /locus_tag="ACHXOX_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR7004432.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHXOX_000882"
     CDS             complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHXOX_000882"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR7004433.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43504..43579
                     /locus_tag="ACHXOX_000883"
     tRNA            43504..43579
                     /locus_tag="ACHXOX_000883"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43537..43539,aa:Val,seq:tac)
     gene            43624..43699
                     /locus_tag="ACHXOX_000884"
     tRNA            43624..43699
                     /locus_tag="ACHXOX_000884"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43657..43659,aa:Val,seq:tac)
     gene            43746..43821
                     /locus_tag="ACHXOX_000885"
     tRNA            43746..43821
                     /locus_tag="ACHXOX_000885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43779..43781,aa:Val,seq:tac)
     gene            43826..43901
                     /locus_tag="ACHXOX_000886"
     tRNA            43826..43901
                     /locus_tag="ACHXOX_000886"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43859..43861,aa:Lys,seq:ttt)
     gene            43932..44057
                     /locus_tag="ACHXOX_000887"
     ncRNA           43932..44057
                     /ncRNA_class="other"
                     /locus_tag="ACHXOX_000887"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44177..44518
                     /locus_tag="ACHXOX_000888"
     CDS             44177..44518
                     /locus_tag="ACHXOX_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR7004434.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(44509..45435)
                     /locus_tag="ACHXOX_000889"
     CDS             complement(44509..45435)
                     /locus_tag="ACHXOX_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7004435.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            45525..46523
                     /locus_tag="ACHXOX_000890"
     CDS             45525..46523
                     /locus_tag="ACHXOX_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR7004436.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46520..46738)
                     /locus_tag="ACHXOX_000891"
     CDS             complement(46520..46738)
                     /locus_tag="ACHXOX_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR7004437.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="ACHXOX_000892"
     CDS             complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="ACHXOX_000892"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR7004438.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="ACHXOX_000893"
     CDS             complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="ACHXOX_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR7004439.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            50042..50803
                     /gene="cysZ"
                     /locus_tag="ACHXOX_000894"
     CDS             50042..50803
                     /gene="cysZ"
                     /locus_tag="ACHXOX_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR7004440.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50988..51959
                     /gene="cysK"
                     /locus_tag="ACHXOX_000895"
     CDS             50988..51959
                     /gene="cysK"
                     /locus_tag="ACHXOX_000895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR7004441.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52343..52600
                     /gene="ptsH"
                     /locus_tag="ACHXOX_000896"
     CDS             52343..52600
                     /gene="ptsH"
                     /locus_tag="ACHXOX_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR7004442.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52645..54372
                     /gene="ptsI"
                     /locus_tag="ACHXOX_000897"
     CDS             52645..54372
                     /gene="ptsI"
                     /locus_tag="ACHXOX_000897"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR7004443.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54413..54922
                     /gene="crr"
                     /locus_tag="ACHXOX_000898"
     CDS             54413..54922
                     /gene="crr"
                     /locus_tag="ACHXOX_000898"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR7004444.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT        14332 a        13802 c        12950 g        13866 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaagcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
    23161 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
    23221 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
    23281 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
    23341 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
    23401 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
    23461 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
    23521 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
    23581 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
    23641 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
    23701 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
    23761 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    23821 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    23881 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    23941 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
    24001 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
    24061 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    24121 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    24181 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
    24241 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
    24301 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    24361 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    24421 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    24481 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    24541 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    24601 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
    24661 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    24721 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
    24781 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    24841 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
    24901 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
    24961 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
    25021 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    25081 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    25141 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    25201 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
    25261 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
    25321 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    25381 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
    25441 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
    25501 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
    25561 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
    25621 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
    25681 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
    25741 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
    25801 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
    25861 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    25921 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
    25981 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    26041 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
    26101 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
    26161 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    26221 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
    26281 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
    26341 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
    26401 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    26461 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
    26521 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    26581 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
    26641 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
    26701 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
    26761 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
    26821 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
    26881 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
    26941 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
    27001 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
    27061 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
    27121 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
    27181 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
    27241 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
    27301 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
    27361 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
    27421 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
    27481 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
    27541 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
    27601 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
    27661 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
    27721 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
    27781 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
    27841 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    27901 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    27961 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
    28021 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
    28081 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
    28141 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    28201 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
    28261 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
    28321 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
    28381 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    28441 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
    28501 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
    28561 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    28621 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    28681 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    28741 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    28801 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    28861 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    28921 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    28981 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    29041 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    29101 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    29161 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    29221 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    29281 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    29341 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    29401 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    29461 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    29521 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    29581 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    29641 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    29701 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    29761 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    29821 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    29881 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    29941 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    30001 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    30061 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    30121 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    30181 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    30241 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30301 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    30361 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30421 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30481 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    30541 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    30601 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    30661 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    30721 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    30781 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30841 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30901 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    30961 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg
    31021 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    31081 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    31141 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    31201 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    31261 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    31321 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31381 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    31441 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    31501 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    31561 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31621 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31681 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31741 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31801 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31861 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31921 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    31981 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    32041 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    32101 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    32161 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    32221 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    32281 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    32341 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    32401 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    32461 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    32521 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    32581 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    32641 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    32701 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    32761 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    32821 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    32881 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    32941 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    33001 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    33061 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    33121 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    33181 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    33241 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    33301 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    33361 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    33421 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    33481 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    33541 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    33601 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    33661 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    33721 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    33781 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    33841 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    33901 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    33961 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    34021 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    34081 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    34141 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    34201 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    34261 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    34321 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    34381 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    34441 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    34501 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    34561 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    34621 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    34681 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    34741 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    34801 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    34861 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    34921 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    34981 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    35041 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    35101 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    35161 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    35221 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    35281 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    35341 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    35401 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    35461 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    35521 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    35581 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    35641 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    35701 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    35761 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    35821 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    35881 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    35941 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    36001 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    36061 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    36121 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    36181 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    36241 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    36301 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    36361 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    36421 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    36481 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    36541 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    36601 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    36661 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    36721 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    36781 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    36841 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    36901 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    36961 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    37021 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    37081 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    37141 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    37201 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    37261 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    37321 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    37381 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    37441 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    37501 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    37561 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    37621 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    37681 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    37741 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    37801 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    37861 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    37921 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    37981 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    38041 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    38101 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    38161 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    38221 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    38281 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    38341 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    38401 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    38461 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    38521 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    38581 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    38641 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    38701 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    38761 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    38821 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    38881 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    38941 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    39001 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    39061 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    39121 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    39181 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    39241 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    39301 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    39361 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    39421 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39481 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    39541 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    39601 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    39661 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39721 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    39781 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    39841 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    39901 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39961 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    40021 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    40081 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    40141 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    40201 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    40261 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    40321 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    40381 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    40441 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    40501 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    40561 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    40621 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    40681 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    40741 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    40801 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    40861 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    40921 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    40981 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    41041 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    41101 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    41161 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    41221 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    41281 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    41341 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    41401 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    41461 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    41521 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    41581 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    41641 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    41701 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    41761 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    41821 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    41881 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    41941 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    42001 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    42061 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    42121 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    42181 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    42241 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    42301 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    42361 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    42421 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    42481 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    42541 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    42601 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    42661 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    42721 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    42781 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    42841 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    42901 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    42961 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    43021 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    43081 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    43141 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    43201 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    43261 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    43321 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    43381 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    43441 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    43501 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43561 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga
    43621 agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43681 atcccgtcat cacccaccaa tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc
    43741 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43801 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    43861 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    43921 aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg tttgagttga
    43981 gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg aagcgagtca
    44041 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    44101 ttgtaagatt gatttttctt ctcaagttcc ttgttcttct gccgatatta cctcgtacag
    44161 tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca tacaaacaca
    44221 gagtagtaac agggcatcac aaggtagcga tgttgcttca aaaatcgcgc ggataagtca
    44281 gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg gcagtgctga
    44341 agacaaacaa aaacaggccg aattgattca acagcaaatc accctactgg aaacacaact
    44401 ggcgcagttg cagagacagc aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct
    44461 gaatgtatcc cttctaaatc ctgttgaaaa tacgactcat atcgatatct atatctgata
    44521 caacggagcc ccttcccaac cagccattag cgcattgccc tggtctcgta ccacgtccca
    44581 taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg
    44641 ttcaacaacc ggcgtaatgc gctttaccac cagtgggcta ccttcaggca atggtagcgc
    44701 cagtgcggga agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg
    44761 atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg ctgcgtcaat
    44821 cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg
    44881 ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg
    44941 ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgca
    45001 aaaatcgaca tctccctggc gaatactttc catcacccac tgctgcggac gatcgtgcag
    45061 gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg gaataagatg
    45121 cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg
    45181 cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa
    45241 ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc
    45301 agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca agccgatacg
    45361 ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgttt
    45421 taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt ttatttctca
    45481 aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta
    45541 tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg
    45601 ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt gccctgctgt
    45661 tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc
    45721 gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt
    45781 ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt ctctacttgt
    45841 gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg
    45901 tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat
    45961 tgctagttgg tctggtgatg aatgttcacg gtgcaggggg cagccttgag caggtcggta
    46021 aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg ccgtggattg
    46081 gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt
    46141 tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat aaagttggct
    46201 ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc gtgattgtag
    46261 ttaacgtctt tatggcacgc cgactgggct tcaataaggc agatgaaatt actatcgtct
    46321 tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca
    46381 catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg
    46441 tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag gcgcagcagg
    46501 aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc
    46561 cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct gccgggaact
    46621 catctttgcg ggcaaaccac agcagatact cctccggcag gtcgattaag cgacgccctt
    46681 tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct
    46741 cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc cagttcctgc
    46801 gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac
    46861 acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc atcacgcgac
    46921 atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct gtcaatctct
    46981 tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag ctcgctgatg
    47041 acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca
    47101 acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcacttc cagcgtgccg
    47161 aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc aagtgcatag
    47221 aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc
    47281 gattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt gaacagatct
    47341 gccggagtgt ggacatattc tttttcaacc agctgatcga tgattttgtc gcccattccg
    47401 tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg
    47461 caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa
    47521 ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa
    47581 agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac cactttatcg
    47641 ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc gttactcacc
    47701 agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc
    47761 ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg gaatttaaac
    47821 gctaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg ttccagtgag
    47881 ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc ttcttccact
    47941 ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg atcgctgacc
    48001 ggcaaccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg cagctcgcca
    48061 ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc
    48121 ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa aactttcccg
    48181 cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca
    48241 ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg aatggcgcga
    48301 atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg ggtcgcggca
    48361 ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag cttcagctca
    48421 cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt gttgaaagca
    48481 agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc atggcgtatc
    48541 tggctgaaag ccgccagcgg cgcagcacct acacgttggg taggcgaatc aggcgtaatc
    48601 agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg
    48661 tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggcg aagcgtcgtt
    48721 cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc
    48781 ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc
    48841 tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg
    48901 ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag attgcagcat
    48961 cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc
    49021 cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag
    49081 gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa tattcatatc
    49141 gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct
    49201 accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat
    49261 aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg
    49321 gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg
    49381 tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcat
    49441 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggat
    49501 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
    49561 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
    49621 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
    49681 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
    49741 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
    49801 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
    49861 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
    49921 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
    49981 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
    50041 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
    50101 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
    50161 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
    50221 tctcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
    50281 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
    50341 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
    50401 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
    50461 gcaaaaattt gcctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
    50521 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
    50581 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
    50641 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
    50701 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
    50761 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
    50821 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
    50881 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
    50941 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
    51001 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
    51061 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
    51121 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
    51181 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
    51241 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
    51301 aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
    51361 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
    51421 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
    51481 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
    51541 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
    51601 caaccgattc tccagttatc gcccaggcgc tggcaggtga agagattaaa cctggcccgc
    51661 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
    51721 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
    51781 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcggcg ttgaaactac
    51841 aagaagatga aagctttacc aataagaata ttgtggttat tctaccatca tcgggtgagc
    51901 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
    51961 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
    52021 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
    52081 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
    52141 atttggttca attcttcctt taacggcata atgtttaatg acgtacgaaa cgtcagcggt
    52201 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
    52261 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
    52321 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
    52381 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
    52441 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
    52501 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
    52561 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
    52621 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
    52681 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
    52741 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
    52801 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
    52861 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
    52921 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
    52981 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
    53041 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
    53101 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
    53161 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
    53221 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
    53281 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
    53341 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
    53401 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
    53461 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
    53521 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
    53581 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
    53641 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
    53701 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
    53761 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
    53821 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    53881 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    53941 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
    54001 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    54061 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    54121 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    54181 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    54241 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    54301 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    54361 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    54421 gttcgataaa ctgaaatctc tggtttccga cgacaagaag gataccggaa ctattgagat
    54481 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    54541 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    54601 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    54661 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    54721 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    54781 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    54841 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    54901 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca
//
LOCUS       DAZRVY010000015        54816 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZRVY010000015 DAZRVY010000000
VERSION     DAZRVY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.015"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            54..1178
                     /locus_tag="ACHXOX_000899"
     CDS             54..1178
                     /locus_tag="ACHXOX_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR7004445.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARSYLQMLPIQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     gene            1190..1843
                     /locus_tag="ACHXOX_000900"
     CDS             1190..1843
                     /locus_tag="ACHXOX_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004446.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSDINNIKEIYSNDDLNCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLEIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            1873..2408
                     /locus_tag="ACHXOX_000901"
                     /pseudo
     CDS             1873..2408
                     /locus_tag="ACHXOX_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            2424..2828
                     /locus_tag="ACHXOX_000902"
     CDS             2424..2828
                     /locus_tag="ACHXOX_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR7004447.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMPFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPERPEPIEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            2969..3556
                     /locus_tag="ACHXOX_000903"
     CDS             2969..3556
                     /locus_tag="ACHXOX_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFR7004448.1"
                     /translation="MYIHIVPKLYHLMANKCSLKSISIPELDFIIDSESLSVGRPWPN
                     KCLWVGMRKGRKSVNGLVLQTDKKLRWFTTIYTWDIEDMGLIYHQVNTYIEDDDFDMV
                     SQEILLNGSFDKWSNRVHSAYENKPPARIQPKMESLLNKPGENSHDVWEEFEWGDFLL
                     SREESLLLHTIQSERLSTDCSLFKRQPSIESALVI"
     gene            3568..5733
                     /locus_tag="ACHXOX_000904"
     CDS             3568..5733
                     /locus_tag="ACHXOX_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFR7004449.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAEKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGKMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWKDFCGKSLTDKQIETLITKGVT
                     SELKGFISQKTNKEFSAKVKLVDKATGKLGFEFTQKSKK"
     gene            5802..6008
                     /gene="hha"
                     /locus_tag="ACHXOX_000905"
     CDS             5802..6008
                     /gene="hha"
                     /locus_tag="ACHXOX_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR7004450.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNSLPPSELESFNAAAD
                     HRLAELTMNKLYDKIPPSVWKYVY"
     gene            6154..6585
                     /locus_tag="ACHXOX_000906"
     CDS             6154..6585
                     /locus_tag="ACHXOX_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004451.1"
                     /translation="MFFLYDTYNFFYYLIKLIVIQPQYICVYMIFFFFNAGIAYSITN
                     DIEDQVCRWLLFVSMLHALMIPLAIIMPPQEILQETEKRQELHESIPKTCKLKALDAQ
                     QGGLFGVDKDEWVFPDNKSFYLPEKYRPENRITELAMMKEG"
     gene            6830..7270
                     /locus_tag="ACHXOX_000907"
     CDS             6830..7270
                     /locus_tag="ACHXOX_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004452.1"
                     /translation="MKEYEKTALIYSLKESNTLVLRIILISILVVGVISGAVYLYNLA
                     ISLFDSNPTSVQFTVCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAI
                     IIISFITMALTASLVLKDSSNIWLNITFIALLLSIFVAVISVWG"
     gene            complement(7313..11470)
                     /locus_tag="ACHXOX_000908"
     CDS             complement(7313..11470)
                     /locus_tag="ACHXOX_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR7004453.1"
                     /translation="MIVRYGGGNDGVVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAVVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVTEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPLQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTHHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNTK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQATEQTQATEQAQATEQAQATSSYDPGVITRANTLDSQML
                     SKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVASFDNENDAKEFCNIVNELG
                     IDRTQALIQEQLTSQHDQATAQEHNKQEIYCVNFSHLHDENEGVVFHSKDAAIDFYEG
                     NKSSAIEKYESNYLNGDGFETIVLMSKTVSTDELSSYPEGGIVTLDKPFEVIANSYEE
                     YRTPVYAVSFSKDEFSEDIKTFDSLHDASEYKNNMLQEHGLNQDDILITPVTREEIAF
                     KGIKDAVNDANMAAMEQEGDSSRESPEEILASISANEHMISGLENFLVKDRSQFSSCN
                     GDIVVEAEITRGEGGLYHLAVAGKHGLERGDAVARVDVTEQQFAAITGKTPSEVLTGD
                     QTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGLNAEIKDFSSLKDAIEWGR
                     VECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERHEIEKAQGRDCTPEDGKIL
                     EAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDALGKQFVQAREEHLELQQQR
                     DNSQDMH"
     gene            complement(11467..11808)
                     /gene="mobC"
                     /locus_tag="ACHXOX_000909"
     CDS             complement(11467..11808)
                     /gene="mobC"
                     /locus_tag="ACHXOX_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFR7004454.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            12149..12628
                     /locus_tag="ACHXOX_000910"
     CDS             12149..12628
                     /locus_tag="ACHXOX_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004455.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIRNKR"
     gene            12634..13335
                     /gene="stbB"
                     /locus_tag="ACHXOX_000911"
     CDS             12634..13335
                     /gene="stbB"
                     /locus_tag="ACHXOX_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFR7004456.1"
                     /translation="MYLKLAIVNNSGNVGKSTIGQTLLKPRLEGSEIIKVETLNTDGT
                     NDEKLAAKEFDEIIKRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVR
                     EAKQQIDSIQTITTLMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGEN
                     PVVVYHNNIFNILNKSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNG
                     FNSDLDVAFKNLNLV"
     gene            13382..13981
                     /locus_tag="ACHXOX_000912"
     CDS             13382..13981
                     /locus_tag="ACHXOX_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004457.1"
                     /translation="MSRSISEEFKDQKINDLLEDYIALEKQIEKTANLIINAIDETPA
                     RLDAVLSDKVNKIIQSSIDINKDINIAKSDIRQISNECQSEITKQAINTFSGIKQNFD
                     QIASDHKKQLEILTKSAKPFSIKTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRF
                     YARAFLDMKKITEESMRKLPKSDQQNIKLKLDEIDSRKP"
     gene            14001..14123
                     /locus_tag="ACHXOX_000913"
                     /pseudo
     CDS             14001..14123
                     /locus_tag="ACHXOX_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14455..14718
                     /locus_tag="ACHXOX_000914"
     CDS             14455..14718
                     /locus_tag="ACHXOX_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004458.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            14729..15049
                     /locus_tag="ACHXOX_000915"
     CDS             14729..15049
                     /locus_tag="ACHXOX_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004459.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(15180..15617)
                     /locus_tag="ACHXOX_000916"
     CDS             complement(15180..15617)
                     /locus_tag="ACHXOX_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR7004460.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(15642..15824)
                     /locus_tag="ACHXOX_000917"
     CDS             complement(15642..15824)
                     /locus_tag="ACHXOX_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR7004461.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            16121..16564
                     /locus_tag="ACHXOX_000918"
     CDS             16121..16564
                     /locus_tag="ACHXOX_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004462.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(16952..17230)
                     /locus_tag="ACHXOX_000919"
     CDS             complement(16952..17230)
                     /locus_tag="ACHXOX_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR7004463.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
     gene            complement(17780..18031)
                     /locus_tag="ACHXOX_000920"
     CDS             complement(17780..18031)
                     /locus_tag="ACHXOX_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7004464.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
     gene            complement(18021..18281)
                     /locus_tag="ACHXOX_000921"
     CDS             complement(18021..18281)
                     /locus_tag="ACHXOX_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004465.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEKAFDTIDFASDGLRENDKEIVSMMIREMSEGKNAK"
     gene            complement(18333..18557)
                     /locus_tag="ACHXOX_000922"
     CDS             complement(18333..18557)
                     /locus_tag="ACHXOX_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004466.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
     gene            complement(18655..18864)
                     /locus_tag="ACHXOX_000923"
     CDS             complement(18655..18864)
                     /locus_tag="ACHXOX_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004467.1"
                     /translation="MKVLIGNINIDNYHMLSALAGIAGFDRSIQFTCEISASIEIMED
                     DFVNKAGILKMLDEFIENDFSIKLV"
     gene            complement(18905..19228)
                     /locus_tag="ACHXOX_000924"
     CDS             complement(18905..19228)
                     /locus_tag="ACHXOX_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004468.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKFDNIYCDQLECLFNEQTG
                     LATRL"
     gene            complement(19809..20138)
                     /locus_tag="ACHXOX_000925"
     CDS             complement(19809..20138)
                     /locus_tag="ACHXOX_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004469.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDCYSTPAIEIESFETAYKGSPEEK
                     HEFTSYY"
     gene            20735..21400
                     /locus_tag="ACHXOX_000926"
     CDS             20735..21400
                     /locus_tag="ACHXOX_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFR7004470.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
     gene            21474..21758
                     /locus_tag="ACHXOX_000927"
     CDS             21474..21758
                     /locus_tag="ACHXOX_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR7004471.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(21776..22372)
                     /locus_tag="ACHXOX_000928"
     CDS             complement(21776..22372)
                     /locus_tag="ACHXOX_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR7004472.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(22522..22644)
                     /locus_tag="ACHXOX_000929"
     CDS             complement(22522..22644)
                     /locus_tag="ACHXOX_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7004473.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGNREVTASLAYESNGK"
     gene            complement(22589..22741)
                     /locus_tag="ACHXOX_000930"
                     /pseudo
     CDS             complement(22589..22741)
                     /locus_tag="ACHXOX_000930"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(24087..25115)
                     /gene="repA"
                     /locus_tag="ACHXOX_000931"
     CDS             complement(24087..25115)
                     /gene="repA"
                     /locus_tag="ACHXOX_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR7004474.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFQVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDSYKALGGQLGHAYNRLVKKDPVTGQFTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGNVIPETAVTVSRLSRLLAEQVCFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKFRR
                     EKAAKRKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            26253..26705
                     /locus_tag="ACHXOX_000932"
     CDS             26253..26705
                     /locus_tag="ACHXOX_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFR7004475.1"
                     /translation="MARKLKTNPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTMDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            26698..26892
                     /locus_tag="ACHXOX_000933"
     CDS             26698..26892
                     /locus_tag="ACHXOX_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR7004476.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIHPQ"
     gene            26938..27891
                     /locus_tag="ACHXOX_000934"
     CDS             26938..27891
                     /locus_tag="ACHXOX_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR7004477.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDIPKQEVISKDNANVTIDAVCFVQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPKELTEAMNAQMKAERTKRARILEAEGIRQSEILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            27954..28397
                     /locus_tag="ACHXOX_000935"
     CDS             27954..28397
                     /locus_tag="ACHXOX_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR7004478.1"
                     /translation="MLWFTLFLLCIALEIITGTGWLLLISLGALSSAIVGFFLPISQE
                     ANICFFASISILASIIKFFYDKKHKKLDTLLVNTGHSRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSESKE"
     gene            28582..29238
                     /locus_tag="ACHXOX_000936"
     CDS             28582..29238
                     /locus_tag="ACHXOX_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004479.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTFLSDTGSDL
                     AEPVTTTTATGKIQSILGGMAEMVSILGTVVPFLVIIIAMIWFLFFSSKREKEELTRV
                     EREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALADESNNVRG
                     "
     gene            29501..29803
                     /locus_tag="ACHXOX_000937"
     CDS             29501..29803
                     /locus_tag="ACHXOX_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004480.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKNIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            29800..30057
                     /locus_tag="ACHXOX_000938"
     CDS             29800..30057
                     /locus_tag="ACHXOX_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004481.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KEESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            30061..31056
                     /locus_tag="ACHXOX_000939"
     CDS             30061..31056
                     /locus_tag="ACHXOX_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR7004482.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPTFFAAIALYVIY
                     LIYEITYAQRDVLMSEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNALSTNLNNFLDTSMSIYGKFQIAEKMGVYLVWGIGYIGGMLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFALFSSTRNMFTAWVGSCLNYILLNLFYSISFSF
                     LISFVESVVPVNGDIKLSIVVYFFLITIISIFLIEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPIFNAGSRSASALVQGLNRGSKLKAG"
     gene            31062..31706
                     /locus_tag="ACHXOX_000940"
     CDS             31062..31706
                     /locus_tag="ACHXOX_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR7004483.1"
                     /translation="MKDKVLSFLFAGIITVTSPSIFASGIPVVDVTAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNIAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLIASENARQEAVSREKFKLMMQ"
     gene            31715..31939
                     /locus_tag="ACHXOX_000941"
     CDS             31715..31939
                     /locus_tag="ACHXOX_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR7004484.1"
                     /translation="MKKFISTLTILSCFLLAACEDKVYDVSYYAEHLEQAQDVVEKCS
                     KGDMSGQNCENAREAIQKEQSGKAFKNMMQ"
     gene            complement(32162..32971)
                     /locus_tag="ACHXOX_000942"
     CDS             complement(32162..32971)
                     /locus_tag="ACHXOX_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFR7004485.1"
                     /translation="MNEPLEAELHSLFSFDIYPGASSEQNTGVKLAMARFYLNVPFEE
                     KDLAKQKGAQWDQEQRKWFVPQGKNPIYFIRWIKELNEHDYNVFSQRFYIAESHQSCW
                     RCKKTTPVFGIFLPRWYKYRDVIWGVDPAEWEDCILDEWYETSSPKGMEYFDSKNNMI
                     YRWLTSRVWWTDLTKIEIISTSALSRINEYSKLYYPSHSKTAKMNYYANHCCHCNAMQ
                     GDFMMFNEPGGVFFPVTYEQAEKIRFHEVNETIFAKASYSLIPEAGGFIDL"
     gene            33019..33654
                     /locus_tag="ACHXOX_000943"
     CDS             33019..33654
                     /locus_tag="ACHXOX_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004486.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTADFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQSLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            33826..34113
                     /locus_tag="ACHXOX_000944"
     CDS             33826..34113
                     /locus_tag="ACHXOX_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR7004487.1"
                     /translation="MLSMIAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSECKSA
                     EKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            34116..35351
                     /locus_tag="ACHXOX_000945"
     CDS             34116..35351
                     /locus_tag="ACHXOX_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFR7004488.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALSPAKPISSNNYQAQTAQKTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKALKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKSLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            35357..35794
                     /gene="pilM"
                     /locus_tag="ACHXOX_000946"
     CDS             35357..35794
                     /gene="pilM"
                     /locus_tag="ACHXOX_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR7004489.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFIH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            35913..36311
                     /locus_tag="ACHXOX_000947"
     CDS             35913..36311
                     /locus_tag="ACHXOX_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004490.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADIIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            36332..36916
                     /locus_tag="ACHXOX_000948"
     CDS             36332..36916
                     /locus_tag="ACHXOX_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR7004491.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEITPPNDEEKTSPDWDVFGDFS"
     gene            36916..37206
                     /locus_tag="ACHXOX_000949"
     CDS             36916..37206
                     /locus_tag="ACHXOX_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR7004492.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFS
                     FGGVCALCYLIYNVIMAFMEKKSWSDVGMALVYCSLAGGALYAGNWALELFK"
     gene            37277..37597
                     /locus_tag="ACHXOX_000950"
     CDS             37277..37597
                     /locus_tag="ACHXOX_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR7004493.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALIAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            37603..39960
                     /locus_tag="ACHXOX_000951"
     CDS             37603..39960
                     /locus_tag="ACHXOX_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFR7004494.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAEGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            40126..40860
                     /locus_tag="ACHXOX_000952"
     CDS             40126..40860
                     /locus_tag="ACHXOX_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR7004495.1"
                     /translation="MENTNYYEHSKKEATKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            40926..41627
                     /locus_tag="ACHXOX_000953"
     CDS             40926..41627
                     /locus_tag="ACHXOX_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFR7004496.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            41617..42756
                     /locus_tag="ACHXOX_000954"
     CDS             41617..42756
                     /locus_tag="ACHXOX_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFR7004497.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQADNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            42775..43830
                     /gene="virB11"
                     /locus_tag="ACHXOX_000955"
     CDS             42775..43830
                     /gene="virB11"
                     /locus_tag="ACHXOX_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR7004498.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            43846..45804
                     /locus_tag="ACHXOX_000956"
     CDS             43846..45804
                     /locus_tag="ACHXOX_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR7004499.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQKCFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            45855..47498
                     /locus_tag="ACHXOX_000957"
     CDS             45855..47498
                     /locus_tag="ACHXOX_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR7004500.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQKATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGDAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGVNSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            47549..48859
                     /gene="pilO2"
                     /locus_tag="ACHXOX_000958"
     CDS             47549..48859
                     /gene="pilO2"
                     /locus_tag="ACHXOX_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR7004501.1"
                     /translation="MSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTFARKN
                     DAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAICELG
                     DLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPEPGWKLYQPESWDISQALPS
                     LTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKEAAAE
                     AARLRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLAECST
                     SGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSFDVSP
                     DPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYELMIQ
                     TSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            48843..49337
                     /gene="pilP"
                     /locus_tag="ACHXOX_000959"
     CDS             48843..49337
                     /gene="pilP"
                     /locus_tag="ACHXOX_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR7004502.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            49362..50900
                     /locus_tag="ACHXOX_000960"
     CDS             49362..50900
                     /locus_tag="ACHXOX_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR7004503.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVQVYIADNSKGQRAVQ
                     AYIGTLNVKYPGQVNITSTTLDVIASRYQDSEKRSDSNSSQHLNSNQEKVISYLAKAN
                     KLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            50891..52000
                     /locus_tag="ACHXOX_000961"
     CDS             50891..52000
                     /locus_tag="ACHXOX_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR7004504.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            52045..52602
                     /locus_tag="ACHXOX_000962"
     CDS             52045..52602
                     /locus_tag="ACHXOX_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR7004505.1"
                     /translation="MSSINILNMRSVFSSLSAHRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEASVPKKNCMAMVNALRSSSAISKINN
                     TSTSTVDAATVCSSDSNTLTFSTDS"
     gene            52669..53151
                     /locus_tag="ACHXOX_000963"
     CDS             52669..53151
                     /locus_tag="ACHXOX_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR7004506.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            53155..53790
                     /locus_tag="ACHXOX_000964"
     CDS             53155..53790
                     /locus_tag="ACHXOX_000964"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR7004507.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            53803..>54816
                     /gene="pilV"
                     /locus_tag="ACHXOX_000965"
     CDS             53803..>54816
                     /gene="pilV"
                     /locus_tag="ACHXOX_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR7004508.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGA"
BASE COUNT        16669 a        11860 c        11213 g        15074 t
ORIGIN      
        1 tgcatttcga agcaagaatc tctaccttcc cccctttttt gtctggaggg gatatgtttc
       61 agaaaatcaa aattcgcaaa atgacaatca gccgggcgct ggacaaatac ctgaagacag
      121 tatccatcca caaaaaaggt catctacagg agttttatcg cgtaaatgtg attaaacgtc
      181 accctattgc tgaacgctat atggatgaca ttaccactgt tgatattgcc aattatcggg
      241 atcaacggtt ggcgcaaatt aacccccgaa cggggcgtca aataaccgga aacaccgtcc
      301 gtcttgaact tgccctgttg tcatcattat tcaatatcgc ccgtgtggaa tggggaacat
      361 gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat cagtagcggg cgcgatcgtc
      421 gcctgacttc aggagaagaa cgccgacttt cgcggtattt caaagaaaaa aaccaggctc
      481 tttacgtcat ttttcatctg gcgctggaaa cagcaatgcg acaaggtgaa atcttgtcat
      541 tgcgttggga acacgtcgat ctgcaacatg gggtagctca tcttccaaca accaaaaatg
      601 gtgctccacg cgatgttccg ctatccagga aagcccgtag ttacctgcaa atgctgccaa
      661 tacagcttaa cggtaatatt ttttcttata catcttcggg attcaaaagt gcctggcgaa
      721 cagcacttca ggaactgaag atagaaaact tgcattttca tgacctccgt catgaggcta
      781 tcagccgatt tttcgaactt ggtacattga atgtcattga agttgccgct atttcaggcc
      841 atcgctccct gaacatgttg aaacgataca cacaccttcg ggcttatcaa cttgtcagta
      901 aactcgatgc ccgacgcaag caaaccagca agattgctcc ctattttgtt ccctacccgg
      961 caacggtcga gaacagaaac gggcaagtcg ttgtaactct gagtgatttc gacctggaaa
     1021 caagcgcagc cacaaaagaa caggccatat ttcatgccag cgtgttgctg ctacgaacac
     1081 tggcacaagc cgcccagcga ggagaacgcg tccctactcc tggcgaactt cctacaaaca
     1141 ttgatgaacg ggtaatgatt tgtccattaa caaattagag gattcactta tgatagaaca
     1201 actacagagt gattttttat tctggatatt tatttacttc ctgatagtag gtttgcttat
     1261 cagaaacatt catcttacca gaaaaattaa atccatcgaa aaaaacaaaa tgttcccagc
     1321 ggaggaatat gctgtcctgg acattgaaca gcatatgtcc aataaatata ttgggcggct
     1381 agtaacccgt tttatgaaga gaactaatgg tgcacctttg ttaattgtgt ttccagaata
     1441 cgataaagga gacaacaatg aagacaaaaa ttctccacca ttccaacttg taaaactaac
     1501 atccgacatt aacaatatta aagaaattta ttccaatgat gacttaaact gttttctgaa
     1561 taatatttcg gcacaaaaaa atttatatac aacgcactct ggacctagcc aggcatggat
     1621 tgaacatgca tatcctttgc aacaagtaac tattctctta caaggcactc gtcatagtga
     1681 aagggaagat ataatcagtc aacttgagat tattctttcc agactaaaaa atggagaaaa
     1741 tacaggtttt gagcatgacg atgattttgg atattcattt cagtatgatg ccgcagcagt
     1801 aggaaaatca tcatttttcg acgaagcggc aggaagaaaa taagaacaac gctctatcca
     1861 ttgcacagac aaattaacat taatctttta tgaaaatctt attaacaata tttatttatc
     1921 tgcttaccgc ttttcagtct gcaaatgcag gtggaatacc ttatacattt aacaaaaaaa
     1981 ttcttcaggg gaacgttatt cgcgttctcg atggcgacac catagaaatt aaaacactac
     2041 ccgcaaaaat tgttgtgtat gaagttccga tccgagtccg attgataaac atcgatgccc
     2101 ctgaaaagaa acaacctttt ggacgctggt caactagtca gcttaaaact ctggttgcag
     2161 gtaaacaggt gacagtctct tattcacata aagaccgcta cggacgcatt atcggtcatg
     2221 tgttcacgac gaacggcacg gatgccagcc gctttatggt tcagtctggc gctgcatggg
     2281 tatatgaacg ttacaacgag gatgagtcat taccggctct gcaacgggaa gcgcaggaac
     2341 aaaaacgcgg tttgtgggcc gatgcaaatc cggttccacc gtgggagtgg cgatacaaac
     2401 ataactaact gatagaggtt tttatggctt tatcacccaa agaaaagaaa gagttacaac
     2461 aacgcttcga aaagcaatta ctacctgacc ttgatatgcc atttgaacta aatggcgaag
     2521 tatataaatt taattttgat aaaaatgagt caggtgacta tatcgatagt tttacacaag
     2581 cagtatgggg aggttatcag gatgccgagg aatgggaaaa atatggtaaa catcaggaga
     2641 cagaattacc tgaacgccct gaaccaatcg agggttggtg cgactgtgat aaatgtaagt
     2701 cgatgatttt tcataaagag aaaaatattt atgtttgcct ctcctgccat aaacaaacag
     2761 agccatttct tccaattgac tggaactcaa acgattacga ttggctacct tatgaagatg
     2821 aatactaatg ctgaatactt caaaatcata atgggggaaa gaaatgcatt cttcttttgg
     2881 tttgccatat cctgctggtc actggtttta ttcatgaggt caacaaatca gagcttcttt
     2941 gagtacgtca aagataaagg aaattactat gtatatacat attgttccta aactttatca
     3001 tctcatggct aataaatgta gccttaaatc tatttccata cctgagcttg attttataat
     3061 tgatagtgaa tcattgtctg taggcagacc gtggcctaat aaatgtttat gggttggaat
     3121 gcgaaaagga aggaagtcag ttaatggcct cgttttgcaa acagataaaa aattaagatg
     3181 gtttactact atatacacct gggatattga agatatgggg ttaatttacc atcaagtcaa
     3241 tacctatata gaagatgatg attttgatat ggtttcacag gaaattcttt taaatggttc
     3301 ttttgataag tggagtaaca gggttcattc agcttatgaa aataagcctc cagccagaat
     3361 ccagcccaaa atggagtcct tattaaataa acctggtgaa aacagtcatg atgtgtggga
     3421 agagttcgaa tggggtgact tccttctgtc cagagaagaa agccttttac ttcacacgat
     3481 tcagtctgaa cgattgagta cagattgctc tttattcaaa agacaaccgt ctattgaaag
     3541 tgcattagtt atttaaagga taataagatg agacttttta tcgcagaaaa acccgcagta
     3601 gcgaatgata ttgttaaggc gcttggtggc aattttaccc gtcatgatgg ctggtttgaa
     3661 agtgataata ctattgtgac taactgtttt ggtcatatta tcgaatcaca gccccccgaa
     3721 aactataatc ctgaatacaa agagtggaag attgaaacac tccctttgcg tctttatccc
     3781 gtaaaatacc agcctgtaga aagcgccgaa aaacaggtta aaacgattat tgagcttatc
     3841 aggcgtgctg atgtgaccga aattattcat gctggcgatc ctgatgacga aggacagcta
     3901 cttgtcgatg aggtcctgga atatgcaggc aacacaaaac ccgttaagcg cgttctgatc
     3961 aacgacaaca cccttccggc agtgaaaaag gcgctggcaa atcccaaaaa taaccgtgac
     4021 ttcagaggac tctatctcaa ggctctggcg cgttctgtag ccgacgcgat atacggttta
     4081 tccatgacgc gtgcttacac aatcccggca aaaactaaag gctataaagg tgttttgtct
     4141 gtcggacggg tccagacacc cgtgcttggc ctgattgtaa atcgcacccg tgccaataaa
     4201 aatcataaat ccagcttcta ctacaccatg accggacatt ttcaacgcgg cgctgatgtt
     4261 atcagggcga actggaaacc tggtgagttc gcaccgctga cagaccgcaa attgcttgat
     4321 aaaacctggg cgaatggaac agcaacatca cttgcaggaa aaccagccac agttgaagcg
     4381 gcagcaacag atgataaaaa aacggctgca ccgttgccct ttaacctggt acgacttcag
     4441 cagtacatga acaaaaaatt caaaatgact gctcaaaaaa cgctggatat cacacagcag
     4501 ctacgcgaaa aatacaaagc aattacttac aaccgttcag attgttcata tctttcagac
     4561 gaacaattca gcgaagcacc acaaataatc gatgccctga agtcggtgtt tgatcagcca
     4621 atggatattg atacgacacg taaaagcaag gcatttaaca gcgcaaaagt aacagcgcat
     4681 acagccatca tcccaaccgt tagcgtaccg gacgtaaacg cactgagcac cgacgaacgc
     4741 aacgtttacc tggcaattgc acaacactat cttgtccagt tcatgcctga aaaggcatat
     4801 caggaagtat ccgtcgccat tcagtgtggt gatgagtcgt tttatgcccg tgccaggaaa
     4861 actacagaca gcggatttga agcgtttctt ggtgtggaaa atgccgggga cgatgaagca
     4921 gaagtcgaaa atgatgattc cgcttttgat cttctctgta aaattcgcac aggagaaaca
     4981 gtgacgacaa aagaagtggt tgtgaatgag aagaaaacaa cgccaccgcc attattcacc
     5041 gaagccactt tgcttgccgc gcttgttcgt gtcgcagatt ttgtcactga tccagtgatt
     5101 aaaaagttac tgaaggataa agatcgggat aaaaaagacg aacatggcgg tattggtacg
     5161 ccagctacgc gggcttccat attggaaaca ctgaaaaaac ggaactatat caccctggaa
     5221 aaaggaaaac ttattccgac agataccggg tatgcgctta ttgatgctct gccggatata
     5281 gctgttaatc cagatatgac ggcattatgg gcggaaaaac agacactcat tgaaaacgga
     5341 aaaatgacca tcgaacagtt cgttgacgaa ctgtacaatg atctgatacc gatgatcagt
     5401 aatgcgaaca gtgctgaaat aaaagtttcc cccagcgcgc cgtctggtca gtcacaacgc
     5461 ctgtcctctc cttgtccttc ctgtggtaaa cagattgtta tcaggccaaa aagctattcc
     5521 tgtacaggat gtgaatttaa gatatggaaa gatttctgcg gcaaatcatt aaccgataaa
     5581 caaattgaaa cgttaataac aaaaggagtt acaagtgaat tgaaaggttt cattagtcaa
     5641 aaaacaaaca aagaattttc agcaaaagta aaactggttg acaaagcaac cgggaaactg
     5701 ggatttgagt ttacacaaaa aagtaagaaa taaatccagc agtagaaatc aaacatcgtt
     5761 gtagctacta taggacaagg atgttcttca ggaattgtac aatgaaaaca aaacaagact
     5821 ggctctttca gttaagacgt tgctcaacac tcgacacact tgaaaagatt attgaaaaaa
     5881 accaaaacag cctccctcct tctgagcttg agtcgtttaa tgccgccgcc gatcacaggc
     5941 ttgcagaact cacaatgaat aaactctacg ataaaatccc cccatccgtc tggaaatatg
     6001 tatattagtt ctcatctatt aatattagat ttttgatcga cacaaacatc agcaatccct
     6061 atacaccttt catagaatga attgaaccct gtatatagcc tgtctgaaat ggatatctgg
     6121 caggctatct taatagtgtt ataggcggta tgaatgtttt tcctctacga tacctacaat
     6181 ttcttctact acctgataaa gttaattgtc attcagccac aatatatttg tgtctatatg
     6241 atattctttt ttttcaatgc agggatagca tatagcatca ccaatgatat cgaggatcaa
     6301 gtatgtcgat ggcttttatt cgtaagtatg ttacatgctc tgatgattcc ccttgcaatt
     6361 atcatgccac cacaggaaat acttcaggaa acagagaaaa ggcaggaact acatgaatca
     6421 atacccaaaa catgcaaatt aaaggcactg gatgctcaac aaggtggttt attcggcgtt
     6481 gataaagatg aatgggtatt ccctgacaat aaatcattct atctgcctga aaagtaccgt
     6541 ccagaaaaca ggattacgga actagccatg atgaaagaag gataaagacc tatgaagata
     6601 ttcaaattgt ttgttctttg tatccccctt ctgtttagtc tttttttcgc tggctacggc
     6661 attttgtcaa aaaatccatt tgaaacacta ccgtgtctgt ccgccagtgc tttttgcctt
     6721 tttctgtttg acatactgag gcgatcattt atgaagtgag gtgtcttgat gttaacgttg
     6781 aggttgagaa agttttgttg gttcttatac ttatagaccg gattaaatca tgaaagaata
     6841 cgaaaaaacg gctttaatat attccttaaa ggaaagcaat acattagttc ttcggattat
     6901 actcatctct attttagttg tcggggtgat ttcaggcgcg gtatacttat ataacttagc
     6961 gatctcgttg tttgattcaa acccgacgtc tgtgcaattc accgtttgcg gagtaggctg
     7021 ttttattgct ctattacttc aatggatatc taatgttgat gcaaattaca gagaaagaat
     7081 tggggagcaa ccaagtacaa taaaaaacat tgtacaatat ttatttgcag ccattattat
     7141 cattagtttc attacaatgg cattaacagc ctcattagtc ttaaaagact cgtccaacat
     7201 atggctcaac attactttta ttgcattatt actgtccatt tttgtagctg ttatttctgt
     7261 ttggggttga ctttttttct taaaaagccc gaaaaccggg cttttttctt aattaatgca
     7321 tgtcctgtga attgtcacgc tgttgctgca attccagatg ttcttctctg gcctggacaa
     7381 attgttttcc aagagcatct tctgccattt cacgagtgaa tccgtgatta agcaaatcgg
     7441 attcggcctt ctccctttcg aacttcagtc cttcccctct gaacttgtcc tcaaagcgat
     7501 caattgcctc aagaatcttg ccgtcttccg gcgtgcaatc ccgtccctgt gctttctcaa
     7561 tttcatgacg ctccacgcgt tcagcatttt tcattacagc gtcaatcccc tgagaaatat
     7621 gttcagactc gacgcgataa atgacagtat cgcgcttatt gagatcatgt agctcgcatt
     7681 caacccggcc ccactcaata gcatccttga gagagctgaa atcttttatt tcagcattca
     7741 ggccttcatg tgttgagaaa tagacataat ccttctgttg ttccagttta ttcacatttt
     7801 caatagccct tgtactgaaa tggatacctg taattaccgg aacacgagcc gatgtttggt
     7861 cccccgttaa aacttcagaa ggtgtttttc cagtgatcgc ggcaaattgc tgctccgtaa
     7921 catccactct ggcaactgca tcaccacgct ctaagccatg cttaccagcg accgcaaggt
     7981 gataaagacc accttccccc ctggtaattt ccgcctccac aacgatatcc ccattgcagg
     8041 aactgaactg agaacggtct ttaaccagga agttttcaag cccggagatc atatgctcgt
     8101 tagctgaaat actggcgagt atctcttctg gtgattctct ggacgaatcc ccctcctgtt
     8161 ccatcgctgc catattagcg tcattaacag cgtccttaat tcctttaaag gcgatttctt
     8221 ctcttgttac aggtgtgatt aagatatcat cctggttaag tccgtgctcc tgaagcatat
     8281 tatttttata ttcagatgcg tcgtgcaatg aatcaaaagt cttaatatct tcactaaact
     8341 catccttcga gaaagaaacc gcatacactg gagtacggta ttcttcatat gaattggcaa
     8401 ttacttcaaa tggcttatca agcgttacta tccccccctc tggataagag gataattcat
     8461 cggttgagac agttttagac ataagcacaa tagtttcaaa accatcacca tttaaataat
     8521 tgctttcata tttttctatt gcagaagatt tgtttccctc ataaaagtca atggcagcat
     8581 ctttagaatg aaatacaaca ccctcattct catcatggag gtgactaaag tttacacagt
     8641 aaatttcctg tttattatgc tcctgcgcgg tagcctgatc gtgttgagaa gtaagctgct
     8701 cctgaatcaa agcctgtgtt ctgtcaattc ctaattcatt aacaatattg caaaattctt
     8761 ttgcatcgtt ttcattatca aaactggcaa cactaaagat atcatcgctg tctccaggta
     8821 catgccccag cacttcccaa atctcatttt catcgctatc cagagatttc aaatagccaa
     8881 attcaccatt tgtgccttta ctaagcatct gtgaatccag ggtatttgcc ctggtaataa
     8941 caccaggatc atatgaagat gtggcctgtg cttgctctgt ggcctgcgcc tgctctgtgg
     9001 cctgagtctg ttctgtggcc tgtgcctgtt cggtggcctg ttccttcaat gcatcacctt
     9061 tctctattct ggattcggac tcctggctat tttcctgtgt ttccacctgc tcaaacttat
     9121 taccctgaag ttctgctttt aattccagca tcatctgttg atactgctcc gggcgtaaaa
     9181 taatcttcat gtcctgctct gcggcaactt cagcgcacat ttttttgaaa tcttcagagc
     9241 cggtaagttt gagtacacca ccaaactttt ccctggaata ctcaagcatt attccgatct
     9301 cttcttttga gggattttta cccgcaacga caaagttccc tttgtcggta aagacaggct
     9361 tttccgattc ctgatcgcga taaacaatcc tggaatcctg cttatccatc acaagatctt
     9421 ttagtttggt gttaaccttt ttcgcctctt ctaagcggcg ctgggaatcc accgctttct
     9481 gaagcgaaag ttcgggttcg tcagcagaaa tggcgttgat ttcatcgaag ttgagatagc
     9541 ttttgagacg ttcaagggct ttcatttcat ctttatcctt atgccagtca gcatgatatt
     9601 gacgaattat gtgtcgcccc tctgtgtcga gttcagaaag acgctcaagt gtggttagtt
     9661 ttttgtaaac aatcacaccc acggcaatat cacgctgttc acgtggtatt aacttcaact
     9721 cgcgtctcat ctctcgcaga tcattataca gcttgccacg ctcaaacctg atagtatttc
     9781 gtaatgtttt ttccgataaa ttcagtgagt taagacgctc acgggcttca tgatgcgtaa
     9841 ggcgtctgta ggttggatag cgcgtatatg gcgtgttttc taactgctgt gaataaactt
     9901 caagcagaaa ggatttcgca tctttccagg caagattaat atgctttgtc agaaaatcag
     9961 atgcgttcat attacgattc cccacagaaa accgggggga gccgtccttt gctgttctga
    10021 ttttatgctg cgctggaata atattaaaac gctcggcggc agaagacaga aacctgacag
    10081 gatcaatatt tctgcgaatt tctgccatcg ttgtaatttc gtttttctct gccgcctcat
    10141 tcagagtctg aaacatcgtt tcacacaaaa aagaagagcg ttcaagactt tttattcctg
    10201 actctgtaaa ttgtctatca taatcccgtc gcacatcctg accaggatgt tctctttgcg
    10261 gtaattgctc tgccagatca cgctgcgaaa tatctgacaa tacactgaca gagtcgggtc
    10321 ttgtgaatcc attgattccg taaaccatac cgcgttgggg catacgtgac aatccgactg
    10381 ttgcttcaga ttgacggatt ctggcaaact ttttaggaca cgacttgtaa cctccctctc
    10441 gtcctgacgt ttgctgagaa tttctttcgt taagtttttc tcttgagtca tattctttta
    10501 tcctctcttg caggaaatca atttgttgta aagggcttaa tgtcttgtaa agttcccttg
    10561 ttttctccgc ctggttaaaa atatgcttaa tctcttggct tgttttattt aaccaggtat
    10621 taagtctctt ttctatctct ttgtgtgttg gtttctcaag tgttaatgtc cttgtctcaa
    10681 tgtaattctg acgaaaaaga ggacttttta agtttgtgta ttttttatct ccaggcaatt
    10741 ttacagccag atactcattg gttttaccag catttcttat acgaagttca ccatactctg
    10801 taacaatatc tttgaataca gagtaatttt caattttctc gttctctata cgcgaaagca
    10861 actcaccttt caattcagag tgatgctctt tgtaaagatc ccccttaact ctacttataa
    10921 tttttccgta atccgcatct ttacgcggat agtctttagg gctatcaaga ttatatttat
    10981 tattaataaa ttcctgtata gcatcaagct gttcaattgt atgccctttg gtaacatctc
    11041 ccactggatt caggaacttt tcagtaatca aatttacttt aggaattaca atatgaatat
    11101 gtggttttct ttcaacaagt tcaccagtgc tattatcctg gatatgacga attttaggca
    11161 aatgagcttc cgcataaaaa gagtattcat caggatgata agcattcatc aataattttt
    11221 tataatcatc aacaacagcc tttaacactt cattcgacac atgactttca tgaaaagaca
    11281 gagtaatgtg caaataacgt tcctgacttt tgttttcaat agaatcaata atcttatctg
    11341 tagtctgtaa atcaccatca agaacgacac gatgatcaag ttcatcacgc gtgtactggc
    11401 gttctgcttt gcgaccattt atcagataat caacgacacc atcattacca ccaccgtatc
    11461 tgacaatcat ttagctatct cctgaaggcg atcacgaata ccgattagtc catttaaaac
    11521 ctttttataa agcgattctg atataatacc ttttaaatga tctgaattta atcggtgcgc
    11581 tatttgattt atgttgtttc ctgcccaact caactgagaa agacaacgtt tgtattctgg
    11641 tatgttacgt ttatcagatg catatgtgtt tattttacca atagtaagca gcctgaaaaa
    11701 ttcagactta ctaatattaa gctcttttat agccctatcg aacggagcat attcttcttc
    11761 ggtaagtctg aatgttacat gaacgttttt gttggttttt ttcttcatat gtcctcaagg
    11821 ttcgtagaac cgcataagaa cggctgcatc gttcggggtt tcaaaggggc gtcccccttt
    11881 ggcacacgat tgtaacatga ccggaacggt cttgtgtaca atcggtatcg tgcctgtgtt
    11941 tgagaaaatt atttgattcc atagcattca cacatcaaat gcacaccata aacatagcta
    12001 atacatagca aaaacacacc ataaaaacca atgacatagc atatacatag catatacata
    12061 gcaaacacat agcatataca tagcaaaaat tgcttgacgt tcatgataga attgctcatc
    12121 atgatttcac tgtcctggag actgacaaat gaacaatgga gaaaataaac tacttggttc
    12181 gctcttagct cagaaggtca agcgaagtaa aactggaaga ataagagagc gttttgctga
    12241 aatagaagaa gcgcagcaac aaggcataag aaacattgat attgttaatg ctcttaatga
    12301 tgaaggtttt gatctaacgt taaagacgtt tgaaaacata ttgcatcgca tacgaaaaga
    12361 aagggccgag aaaaaagatg tttctcatct tctttcaaat aaagagaaaa cgtatcaaaa
    12421 agcaataacc attgaggaca aaaatagaaa aacgaagcaa gataatgata tacttaatgc
    12481 atatttgcca gtttgcttca acaatgcaaa gatagctcaa caagctatag ataacaatgt
    12541 atctatagaa acaataaaat cttggaattg tgctaacttt gtacaagtta gcaatactct
    12601 tggaaattat atacgaaata aaaggtaaga aaaatgtact taaaactagc aattgtaaat
    12661 aatagcggaa acgtagggaa atcgaccatt ggtcaaactt tattgaaacc tcgtctggaa
    12721 gggtcagaaa ttataaaagt agaaacatta aacactgatg gtacaaatga cgagaaatta
    12781 gcagcaaagg agtttgacga aattatcaaa cgtattgatg atagtgaatg caccatcatt
    12841 gacgtaggct catcaaatat tgagcaattc cttgtacaga tgaatgaata tcagggaagt
    12901 catgacctta ttgattattt catcgttcct gttgtgcgag aagcaaaaca acaaatagac
    12961 tcaattcaaa ccattacaac actaatggct atgggtgttg aacaggatcg ttttaaagta
    13021 attttcaatc ttgctgaaaa ggacacccct attaacaaac aatattcagt tttcttagct
    13081 gacgacgtat gtaaagaaat tgtaggtgaa aatcctgttg ttgtttacca taacaacatt
    13141 ttcaatattt taaataaaag cggcctacag tatgatgatg tctataatga caatcgtgat
    13201 tttcgttcat taattcgttc agcgtcttca aaggaagagc ggcaagattt gtcaaattta
    13261 agagcagtaa aaatgttgat gaatggattc aacagcgatc ttgatgttgc ctttaaaaac
    13321 ctcaatctcg tctgatacaa tatggccggg atgtacccgg tcataaaatt ggagaaaaac
    13381 aatgtctcgt agtatctctg aagagtttaa agatcaaaaa ataaacgatc ttcttgaaga
    13441 ttatattgct ttagaaaaac agatcgaaaa aacagcaaac ttaattatca acgctataga
    13501 cgagacacct gctcgtctgg atgccgttct gtcagacaaa gtaaataaaa taatacaaag
    13561 cagcattgat attaataaag atattaatat tgcaaaaagc gatataagac aaatatctaa
    13621 tgagtgtcag agcgaaataa ccaaacaagc aataaatact ttttctggaa tcaaacaaaa
    13681 ttttgaccaa attgcttccg accataaaaa acaactagag atactaacta aatcagcaaa
    13741 accattcagt atcaaaacag ccattgcaat ttgtgccctg tgcacattat gcctgtctgc
    13801 ggcattttct ggcgcaacct ggtatgttgc acaacatgaa aaagaggcaa gtctgcgctt
    13861 ctatgcaaga gcatttcttg atatgaaaaa aataactgaa gagagcatga gaaaattacc
    13921 aaaatcagac cagcaaaata taaaactcaa actagacgaa attgattcaa gaaaaccata
    13981 aatatgaaaa ggagtcccta atgaccacat ttaccgacga ggacaaagaa ctaattaaag
    14041 aaatcagaga gcgaattggc agcctggacg tgcgagacaa tattgagcgt cgggcttatg
    14101 aaattgctct ggcatcgctg gaagcagagg cagttatgtt ctgtatatca ggacaaaatg
    14161 tagattcaga agaacatgta tcaaccagca aagcggttgt tgatgcctgg gttgaagaat
    14221 ggaatcaggt tgacggaagt cctggcgaac cactgtacaa aactatgcca ctctactatc
    14281 acgctgcctt gccagcgccg gtagtgcctg aaggactggt taaagcggtg cgtttttatg
    14341 agcaggtaaa aaatgagaat ccggcagtcg aaaccgtagc atggaaagac gctgttgact
    14401 gggttctcga agaggcttgt caggctgtaa acattggcat caaaggagag tgatatggca
    14461 actttaacaa aaaaagaacg ggcatggttg aacgaattac aggacgttct tgatcgctgc
    14521 ccatcaccga aaaaaattgg tttttacacc attggcgata aaagcattta cctgtatgac
    14581 ctgcgccgca tggatgaaat catggaggct cttgataatc gttcgtcgat ggattggtgt
    14641 gttgctgtcc atgatatgaa tgccggattt gatgaaaaga ttttattccc ctcatcagtt
    14701 gaaagcacag caggataagg actaacacat gacaacgttc accgacaaag aactgattaa
    14761 agaaatcaaa gagcgtatag gcagcctgga cgtccgagac aatattgagc gtcgggctta
    14821 tgaaattgct ctggcatcac tggaagcaga agcgccaaat ttgcctggtg gtttcacaat
    14881 tgaagaagca aaagaattac acgaaaatct ggtaaaaagt cacgtaagca aggcattaag
    14941 tggcgaaaaa atgaaaaaag aaaatcgtga tgccgattta cgctggattc atggcgtaat
    15001 agttcaggct gcgtggtttg tgaaagcatc actagggcag aacgcatagt gcgtacctgc
    15061 ttttctcctg gcaacggtgg atcttccgct tcctcgtcgc tcgctcgcta tgctcggtcg
    15121 ctggactgcg gcgagcggtt agctatcaaa aaaaatcccc gcacaacgcg gggatagttt
    15181 tatttatatg caagttcatg acgtgccgcc tgagccagaa aatggctacg atctttgtat
    15241 acgccaccac tttctctcac atatccatca atacgacgaa tcaatacatc aggcaatgaa
    15301 atattaatac gttgttgttt gccctcaatt tctgacaaat caacatcaat tacaaaccag
    15361 ctatcacagt ggttataatt tgggttggcg gaataagtca aacacccttc atcatgaata
    15421 tcatcaacag aatgagcacc gcttattacc atctcttcca cgattgccaa tatcgcttca
    15481 cgcgccattg cgggtatttc tgcctgagaa tcagcagcag aaacacatcc aaaatcaaag
    15541 caatcaaaaa ccggaacggt gataccgtat gctgtatgat catctttcgg tgtttcgaca
    15601 ccaacagaaa agaacatggc tacctccaaa gtcggcgggg attaaatccc cgccattttt
    15661 ttgatcgatc tgacagtacc tagcggcaag tcctttttag gatgaggaac aggaaaagtt
    15721 tttcctgtta ttggcgacca ccatatctgg tgactgcttg ccttatgccg tttcaactcg
    15781 caaccagcag ctatcagttc tttcaacagg tcagcagatt tcatatgtcc tccctaacct
    15841 gaggataatt atacacacgc atacacacaa aggcaaatta tttcattcgt atgacttatg
    15901 aaccacactg taaagacagc tcaatcttta cagtgtaagt catttatcca gttttatcgc
    15961 tgtgtatgtg atacatagaa aataatatag tattttacta tatgatatct gattgacact
    16021 tttaactgca catctcatca tttacatcgt aaagacaaac atagatttac aatgtaaagc
    16081 tatgtaatta aacactccgt gtcccaaata aggaaagaaa atgactcaac taatcccaga
    16141 aaagatactg gaaatcatag atgatcatga tcgtgcagaa aaaaagcaac gcaacaaaat
    16201 cggtttcatt tatttatgtc tctgtttagc aataattggt gtggccgcgt atacctttat
    16261 atcaacgttt agcattaatg tggtaacaat catcataatg cttttcggtg tgtttatgat
    16321 tgtgattttt aagagctatt tagactcatg gagtgggata attcttagca gtgaccacat
    16381 attgtccata ctcgataaaa ccaaagatta cccagaaatt aaacgtatag ttattaatcg
    16441 tctgctctcc ggctcaatac tcacgggtaa agatgaagag tacatataca gccaactgga
    16501 aaaggcccaa caaagcaacg aacgtgaaaa gagattgcaa gcaattaaag agtatacatc
    16561 gtaatacaac gcattacgtc gaaataccct ggatcatcga aaccacatac ccctaagcgc
    16621 agcgcaaccg ccggaaaacg caccacactg taaagacaat aaaatcttta caacgtaaag
    16681 ctaactatgt aatacaacgc gctacacaat aagaaaggga gagcatgaca ctctccccat
    16741 atatgactca aactaaagca ttatcccggt tatgcgccca ccaaatgatt tcgctaataa
    16801 tccatgcttc tatcgcgggt gtttttacag gttccccatt tacaatgcgg tttgcaagct
    16861 ctatcatttc acatactgac atttgagaat ctccaaagat gcatctgtca tttggtaaca
    16921 ggctgcccca cagcctgtta ccaaccccaa attaatattt gctgattact ttaccctcct
    16981 taatcatttt tattagttcc tgttcagaat attcttttcc gtgttccaat gtggaatata
    17041 tattgccaat agtccatacc ccacactttt taatttctgc atttagccag gattcatgca
    17101 gaataaacaa cggttcgcaa tcatttcctt tttcataaac ataaatagat tcggtatcat
    17161 ttccagaatc agaagtacca acgacttttt ctatatcgaa atgttttttt agaaaagttg
    17221 tgaaatgcat tttcatgctc ctgccgctga gcggctttgg tggttgggga ccatcccctc
    17281 ccgatgtgtt tataataggt cagaactgac ctattatcaa gtatttttcc ctccattctt
    17341 caaaaaaaac accatcaaat atagacagtt acatccatgt gatgcccgac agggcatgga
    17401 aggggcgaaa gaaaatacca gggagcagcg gggccacatc gccccgaagc gcggcgcagc
    17461 cgctgacgaa cataccagga tgcacagggc catcccttcg ccctgcaaag ccagaacaga
    17521 ccaacaggcc atgatcatgc tgatttgcgc cgcagaatgc cgcagttagc gcgagcgaca
    17581 gcgagtttac gccgtcctgg tgcattgcag cagggcgggg cgctgtactg cgcgtgtgag
    17641 cgtcagcgag ctacgggcgc aattgctcat tgttcaatgc aactggggct attaagtcca
    17701 gccataaatt cagttatggt ttttgtagct tattccgtga tttcgtcata cgttaattct
    17761 attttcatct taatattcct taattacttc cagaatcaga attcttttta ttccttttac
    17821 aaagtacata atcagggtgt tctccagcta aaagcagaag aaatttaaac tctactggtg
    17881 taagtgctgt accgcttgca gttccgacag gttcttcttt gcgacgccag gtacgcggac
    17941 tcatgctaaa tatttcggca cattctttct gtgtcaggcc aattttttgc cgcagacgtt
    18001 taatgttttc tggtgtgttt tcatttggca ttttttccct cactcatttc ccgaatcatc
    18061 atagaaacaa tttctttatc attctctctt aatccgtcgc tggcaaagtc gatagtgtcg
    18121 aatgccttct catgaaaaac aagatcggca acagttttca ccgttgcttt cgtttttcct
    18181 aaagtcgcag acttagaaaa agtttttact cgcttcacca gatgaaacgt atcaccttct
    18241 ttattcagat aatattgtct gtcacctttt cttatcttca tatcaagata gtctcccctt
    18301 ttaacagtaa aggggcatat agcccctttc ttttaacttt ttccggtaat caaatcgaca
    18361 tcttcctgat aaagctcccc aagaaccgga acaacgccct ttttataatc acggatatta
    18421 tggcagtctg taaaaatgac gacataagcc tcgcctgttt tttcatgttt aaggcaaatg
    18481 gtgcaaagcg tcacgcgcac aacagaaaac acatgaccag ttgtagagac gataacagga
    18541 aaagtagatt gtttcatatt taatctcctg ccgctgagcg gctttggcga ttggggacca
    18601 tcccctctca atgcattaat aataggtcac aaatgaccta ttatcaagta ttttctagac
    18661 gagctttatt gaaaaatcat tttcaataaa ttcatctaac atctttaata taccagcttt
    18721 atttacgaaa tcatcctcca taatttcgat acttgctgat atttcacatg taaactgaat
    18781 gctcctgtca aatcccgcaa tcccagctaa tgcagacaac atatgataat tatcaatatt
    18841 tatattacca ataagaactt tcatttatcc tcctgatatg gggggaatcc ccccatatta
    18901 aaaattacaa tctggttgca agtccggttt gttcattaaa taaacattca agctgatcac
    18961 aataaatatt atcaaatttt gatatttctt ttactgtagc tccacggaca cgggaaaatg
    19021 tcaccaggta tgtatcagag aaggttaatt tgattttaac taaattaatt cccttcattg
    19081 caaaatttga aggcaaacga aagcaaagac cacactcacc attttcatca aaacaggcaa
    19141 agttctttgc gcctgtcata gcaataaagc ggtttccacc aagctgatta agaatttcag
    19201 tagcaacaaa tttggcgtgg ttttccattt ttaagctcct gccgctgagc ggctttggtg
    19261 attggggacc atcccctctc gatgtgttta taataggtca gaactgacct attatcaagt
    19321 attttttcct cctttcttta aaataaatat cgttaaatac aaatatttac attcatgcaa
    19381 tgcccggcag ggcatagaag gggcgaaaga aaatacctga gagcagcggg gccacatcgc
    19441 cccgaagcgc ggcgcagccg ctgacgaaca taccaggatg cacagggcca tcccttcgcc
    19501 ctgcaaagcc aggacagacc aacaggccat gatcatgctg atttgcgccg cagaatgccg
    19561 cagttagcgc gagcgccagc gagtttacgc cgtcctggtg cattgcagca gggcggggcg
    19621 ctgtactgcg cgtgtgagcg tcagcgagct acgggcgcaa cattacattg ctgcattcgc
    19681 ctggcattct ccacgccttg aaggacggag ttttactgcg caccaaataa aaaaggggcg
    19741 attaagcccc tgtattcact attaacaatt taacatggtt gcaggggata ccccctgcaa
    19801 ctattttctt agtagtatga tgtgaactcg tgtttttctt ccggtgatcc tttatatgct
    19861 gtttcgaatg actcaatttc aattgctggc gtactgtaac aatcacgcac tcgccgtttt
    19921 aatggctcac cacagaaatc attgagtaat ggggtcttat atccgctcat tgaatgggtc
    19981 cgatgaaccg aagcgcgaat ctcctgaaca gttacggttt ttttgccatg aacagaaata
    20041 acctgataga aattaacgtt cgtttgctca tatccccagg aagatacaaa cacatcgcca
    20101 acagaaacag tgccgttatt aaaagttaat gttttcattt ttatctcctg ccgctgagcg
    20161 gctttggtgg ttggggacca tcccctcccg atgtgtttat aataggtcag aactgaccta
    20221 ttatcaagta tttttccctc cattcttcaa aaaaaacacc atcaaataca tatagttaca
    20281 ttcatgtgat gcccgacagg gcatggaagg ggcgaaagaa aataccaggg agcagcgggg
    20341 ccacatcgcc ccgaagcgcg gcgcagccgc tgacgaacat accaggatgc acagggccat
    20401 cccttcaccc tgcaaagccc aacggactga caggccatga tcatgctgat ttgcgccgca
    20461 gaatgccgca gttagcgcga gcgccagcga gtttacgccg tcctggtgca ttgcagcagg
    20521 gcggggcgct gtactgcgcg tgtgagcgtc agcgagctac gggcgcaaca acacaccagg
    20581 caaaatcgca tcctgaaaag ttaccctgta acatacacac aatgtaaagc acaaaaacaa
    20641 ttacattgta aagacacaaa catctttaca acgtaactat aatgaataca atgtaaagac
    20701 aaataaacct ttacactgta tatccgaggt ttctatgggg agagcattac ttgtcgcatc
    20761 agataaaggc ggtgtaggaa aaagcaccac agcaacaaac ctggcttctt atctggtaaa
    20821 taattcacga acggtcatca ttctgaagac cgataaaaac aatgatattc ttagctggaa
    20881 cgaaaaaaga aagcagaatg gcttattacc ggttcctgtt tatgaagagt atggcgatgt
    20941 atcagaagtc attaagcgcc tgaaaaaaat atgtgaagtg cttatcattg actgtccagg
    21001 gcatgacagc aaggaatttc gcagtgcctt aactgtagct gacattctgc ttactctcgt
    21061 taagccgtct tcagatttcg aagcagaaac gctgactcat gtaacagaaa aagttcgcac
    21121 agctcaacag atcaatccag acctgcaacc ctgggtactt ttcacgagaa ttaacacgac
    21181 aaaaccacgc cacagaaaag cagcgattga tctcgacaag ttactccggg agaacccggt
    21241 gtggatacag ccacttcgta cacgtatttc agatttagat atttttgaag cggcctgtaa
    21301 tgaaggcgct ggcgttcatg atgtacgtcg cgcatccagc ctttctacag ctaaagcaca
    21361 aatagagctt ttggcgcaag agttaggcat caatccctaa tggatgtaat acgatagctt
    21421 tacatcatta actttacaca gtaaagctaa tgtaacacac gaaaggtgat ataatggctc
    21481 ttgacttgaa aactccggta ataccaggac aaaaaacaga acaaaccaat gcagacacag
    21541 cccgattcat caatgaagct acccgcaaaa aatcgggagc aactaaagtg accaatgtcc
    21601 ggcttgggga cgtttacgaa gatattctgg aacgggaagc actgcgaaca ggccagacaa
    21661 aaacagcaat aatgaaagcg gctcttgcag cttacgaaca cggcctggac gaaaacggaa
    21721 aaaatcactg gattctacag tcagccaggc tgaaatgata aagcccttcg gggctttatc
    21781 ccttacgttt taattgtttc ttctcgctgg ctttcttacg ggcatcgaga accagttgcg
    21841 cataagttgc gtgtgctttt tttactgtac cagcaggttt cccgtaaagg tcgtaacgtg
    21901 ttgcgcctgg tacagtacaa ttctgaagat attgccaccg gaacgtcatc acctgaaccg
    21961 cacaattcca ctcttcaatc gtcagatcca catccgggtt atccgccagg tatttctcaa
    22021 cgtcctggag aatcccgctt ttcaggggca agttcttttt ttcgtctgtc catagttcag
    22081 gaaaaacagc acgaacttta ttgaaacact tttcaaaata ttctttcgga tattttgggg
    22141 gaaaagtgat ttgttttttc aaataccagg gctgaggctg ctttttatgt gttttctctg
    22201 ctacagcatc tttcttctgt actttcttgt tttcattaac tgctgatttt tgtacttcct
    22261 gcggcttttc attcaccaca ggattttctg gcatgaccag aacgcggcgt tttttgacca
    22321 caatcacggg tgtttttttt ggctgcatac tcgcctcaat aattgctgtc atattgacta
    22381 attataccat tttcagcagt taatatcctg aaaatacatg gaaaaagggg gtatccgtac
    22441 agaatacccc ctttgcaaag gcgcttaatg ccagaaccac atcatcccca aaaaggcggg
    22501 ctttcgcccg cctccgggtt gctacttacc gttggattcg taagccagac ttgccgtaac
    22561 ctccctgttt ccatctttca gccggacctc gcacaaacga ttccgggtca gatacgtgaa
    22621 tattaacaat gtgcagcaca cgattaataa acaccaggtc agggcgtttt tcggctgttt
    22681 catggccttc ttctccttgc gtaacgcgcg gtaagaggct atcctgatgt tgttgaagca
    22741 ttgggatcgc ctcgtgggtt aatgaaaaat taactacggg gctttcttct ttctgccgca
    22801 caacaagata caaaccacct tttcgccatg aggcagaaag cctcaagcgc caacagcatt
    22861 atatattgac caggaattaa attcccagct ttcggcgtgg actatacagt gtgtgattca
    22921 ccacgatgaa tcctttgttg atccactgtg ttacctgcgc aggttgaact ccggtcagcc
    22981 tggcgaacga tgcctgattc ccccgataga acttgttgat gtattcaatc aaaggcattt
    23041 tttaattctc cgaaacaacg caaaatcata tagtaattta ctatatgatt tttattctgc
    23101 aagtgattaa ctcatcggat ttctttgtag ttaacgaaac gttaaattta gtttgtgcct
    23161 ggaatggcac gcaagaagca cactagcatt atcattttga tcaaaacgct gtttcccccc
    23221 gttattgggg ggctttcttt ttgatgccgc gccagcggca tgtaaggcgg cactctgttg
    23281 ttttatatgg cacagagcta aaccgccgaa gcccggcgta agccggtact gattgatagg
    23341 tttcacctta cccatcccca tcctgctaag ccatacccgc tttcagccat gaaatagctt
    23401 ttgtgcgcgg tcggagtggt cccggcaagg gtttacccgc agtcggggcg tatctccgcg
    23461 ttagcggacc gtcagggccg cttacgagcg tgtatttggt acttccagcc agaaggctga
    23521 cagcgatgag atttcttagt tacaacatcc attattgaaa gcgatgttgt tcccgccctt
    23581 tgggcggggg cggcggcgct tttcagtgat gggttgggcg ctgttgctga gtatttgcag
    23641 cgtttttttg ttggagggtt ggaggggctt tgtttttcat tgggcagaag tccatatgcc
    23701 cgccgcgaag cggtgggagg tacttttata ttctgagcgg cgcggcggta gccgctgcaa
    23761 gcgaaggcat ttttgtgatt atccacagtt gtttcaaaga taaatatttt ccctgcggtc
    23821 aacgcagggg attaaagaac aggggttaag aatatattgc ggatttgatc ttctacgttt
    23881 gctgctttct gaagttattc gtttgcgggt ttttgttaat aaccgaaggc ggtttagctc
    23941 cgatacccga ttgtggccat caaaaaactg tcagagcggg acaaaatgaa aaacggtcgt
    24001 caaaaatgag gaatttcatg aaatgcaggc aacacgaaaa gccccgaagg ggctgaagag
    24061 agtgccgatt gtatcaggca gctaaactag tggggtggcg aagctgtcag ggccagttca
    24121 agctgacgta gatccctggc taccagttgc tctaatcttt ctttagtgca ccagtacgct
    24181 tcgtccggcg gcatacgttt cagaataaac cgtgcaattt cgtttcgctg ttcatcatac
    24241 ggcagttttg caaggcgatt ggcacgtttg cgctttgctg ctttctctcg tcggaacttg
    24301 atggcatcga gagaacgctg cgcataccag cgtttacgag ccgagtgaac agagatttcc
    24361 tcgccctcgc ggataacgcc ttctttaatc agttgcagac ggatttcact ttctgcaagg
    24421 cgtttacgtt gctgttcctg aagtttcacc agatcaactc ccagcatttt ccagccagtt
    24481 tcggtgatcc agacgtattt cggcagtcgc tggcgggatt cacgatccca tatcgtcttt
    24541 tctgacgttc ccaacgtgcc aaagcaaacc tgctctgcca gcagacggga aaggcgcgat
    24601 actgtgactg cggtttcagg aataacgttt cccttactgt cttttggact tatttcttct
    24661 gccaggcggg agacagacat gccgacagtg tgcaatccac gatcacaaaa actcacaagt
    24721 acaacccata ttgcgtcaag cagccgctgt ttctccggtc ggaatttata tttgcgacca
    24781 acactctgac gtttcagtac ataaaatgga tgcaacgaca tgtgcattct gagtgtgaac
    24841 tgaccagtta ccgggtcttt tttaacgaga cgattgtaag cgtggcccaa ctgtccgccc
    24901 agcgccttat agctgtcggg acggaaccag gctggattct cacactttgg cttagtggaa
    24961 tgttcacccc ggcgacgttt tgttgttccg cctttgtttt cctgggggac ctggaatgag
    25021 ctttcaatgc ccgcttcgta gtcctgccag aaacggacct gctcttccgg tgatagttca
    25081 gaccagtgac gtttgctaat cacagcaagc tgcacttagc ttgcactgta agagttgata
    25141 tttccttctc atggaattaa actccatttc aggtaacaag catacctgaa ataaaacgtt
    25201 atcagctcaa ggctcggcct tgagggcaac ccccgctaat ctacgctgcc tggaaaacac
    25261 gagattacgg gggttttctt tatctgaaat ttacgttctt gaacaaaatt tcagcacctc
    25321 gttttctgca tttacaaatc gcggaaaact tgcctgcaaa tttagcagca tgggcgcaat
    25381 cgatcaacga tgttcaagcc tggctcgttt cctttttgat tcagcacaca tctgtgactg
    25441 cttcaaaatc gttttcagaa cagctactga agccccttct ttactgacgt tccccataca
    25501 aaaccttaca aaatcagaaa ttcagaattt tgtatattca aaaaagtttt tttgactgca
    25561 tacacccatt cacacgatcg aataaaccac acaaacagta ttatctagat ttttagatcg
    25621 tacatcattt aacaaacaga atattattct gttttttaga tgttatccag atcattagat
    25681 cgctcttgtg gcattgacag gttttaaatg tcgcacacac aatactatcc agatgattgt
    25741 aaaaagctcg ttcatcgcgt tttttgtgct cattgcctgg aaaatgagca ctctttgacg
    25801 cccgttagaa cgcttgtcat ctcatgctct ttaacaaccc cggacagcgg tcattgccta
    25861 aagccctggc ataaggaggt tattattcgt gcatttcaag caccgctggc atctgtcagc
    25921 aacgggctac gtaacagagt gacgtgccgc gccatctaca caggtaacga ctctttatcc
    25981 gcaaatatca ttctgcatac ttaaaccccc ttatttggtg aactcgcgtt gagttaatct
    26041 cactcactct ctccctgagc aagtcgtaca ggctgcaacg cagaagtaca ccagatttgc
    26101 cagtctccac tccaggtaag ggggtttagc tatgcatgat gatgaacaat cagataaaga
    26161 gcctgtagtg agtggcagca acttttcata attagcttat gacgctgtat tccggggatg
    26221 tttctatttc agacaaacaa ccgggagaaa atatggcgcg taagttaaaa actaatcctg
    26281 tcattcctga atgtgtggca gatttgaaag gatatccact ctatatcatc gtggcttatt
    26341 ggggattaag aaataataaa acgctcacaa caatggatgt tagccgtaat ttccttatta
    26401 cacagcaaca atcttatgat gtgcttacct acatttacca ggaagcacag aagcagataa
    26461 cagcaactaa acgaacacta tttaacgagc acaaaagaag aattagcgcg ttcaggattc
    26521 ttgatattga caaaggcata ttgcgcacac ccaaaaaaga gattgcatat gccagctacg
    26581 acaatatacc aactgtcaca atgaaaagta cccgttgtga gatccaggat agttttacag
    26641 aactgcgccg ctggatgtgt tcaagaaaga caggtgatcg agtggaggta tttttgaatg
    26701 cctaagtacc aaataaacgc aaccatcaaa aaaccaggtg gtggccccgt gaaatggact
    26761 cattacagcg acttccctat gaccgctgaa gaatgcagaa aaaacttctc aaaagaaaaa
    26821 gaagcaggaa agtgtttttc tgttgatgtt catatttcag atttcatttg caaagaaata
    26881 catccacagt gatgaattgg gagagggtta atcatgattg acgctataat tacgccaata
    26941 gttacatcta ttccgttact cttattaatt ttagttgcgc tgatattcgt taaatctgcc
    27001 gtcaaaatcg tgccacaagg taatgcctgg acagtggaac gctttggtaa atatacccat
    27061 acgctttccc ccggtcttca cttcctgatc ccgtttatgg accgcatagg tcaacggatc
    27121 aacatgatgg aaaccgtcct ggatattcct aaacaggaag ttatttcgaa agacaatgca
    27181 aacgtaacta tcgatgctgt ttgttttgtc caggtcattg atgcagcgaa ggcagcttat
    27241 gaagtcgata atcttgcatc agctatttca aatcttgtta tgaccaatat ccgaactgtc
    27301 gttggtggaa tgaatctcga cgatatgctt tctcaacgtg acagcatcaa ttctaaactg
    27361 ttaacggttg tcgattatgc gactgacccg tggggaatta aggtcacacg tattgaaatt
    27421 cgggatgtta aaccaccaaa agaactgact gaagcgatga acgcgcaaat gaaagcggaa
    27481 cgaactaagc gtgcccggat tcttgaagcg gaaggtatcc gccagtcaga aattctgaag
    27541 gcagaagggg aaaaacagtc tcaaatactg aaagcggaag gggaacggca atcagcattt
    27601 cttcagtctg aagcacgcga acgtcaggca gaagcggaag ctcgcgcaac aaaactggtg
    27661 tcggatgcaa tcgctgaagg tgatgttcag tctgttaatt actttattgc gcagaaatac
    27721 actgaagcac tccaggcaat tggcacagca tcaaacagta aactggttat gatgccgctt
    27781 gattcaagta gcctggtgag ttcggttgca ggtatcagtg aactgctaaa aaacgtatca
    27841 caggatactc ccaaagttga tatgaaacag gctcaacaat atttgtctta aaaaagatag
    27901 cggacggtga caatattgtt ttaatcaccg tccttatctc atggagatta gccatgttat
    27961 ggtttacact gtttcttctg tgtattgcac ttgaaattat aaccggaaca ggatggttac
    28021 tattaatcag tcttggtgca ttatcaagcg ccattgtggg cttttttctt ccgatatcac
    28081 aagaagccaa tatatgcttc tttgcgtcaa tcagcatact ggcgtcgata attaagttct
    28141 tctatgacaa aaaacataag aaattagata ctctcctggt gaacacaggg cattcacgtt
    28201 ttaaaggaaa agaatttacg ctcagtgacg atattgttaa cggcaagggg caattactca
    28261 ttggtgatac attctggcct gttgagacgt tacacgatga aaactaccct gcgggaacaa
    28321 gagttattgt tacagacatg caaggaataa ctcttaaagt aatgaccatc gaagacaaga
    28381 gtgaaagcaa ggaataaaac atgcctcttt cgttgttaat tccgcttacg ggaacagcga
    28441 tgaatgcact atgtattttt attatcagac ttctggataa agattttatt gaagtctaca
    28501 aaaaaacgct aataaatatt actgcattac atattttatt gatggtgtta agtgtcacag
    28561 gaatagtatg aggacaattc tatgcgaaac gcaaatgaag ggatttctct tgcagctatt
    28621 gtagctaaag aaacaacaac aggtaaatgg aatggtactg gtgggggtat tggtttaggt
    28681 actggtggta ttggcttatt catcggaggt gtttctggtt ctaaacaaga gcagacacaa
    28741 agggcaaaaa actttgaatc gcctaaagag gaagaataca gtctattaaa tgtatacggt
    28801 tcgataataa aagttctcgt ttttggcgtg ctatttggtt tatctgcccg tttatttaca
    28861 tttttatcag acactggttc tgatcttgct gaaccagtaa caaccaccac agccacagga
    28921 aagattcaat ctattctggg tggcatggca gaaatggtaa gcattttagg caccgttgtc
    28981 ccttttttag taattatcat agcaatgatc tggtttctgt ttttcagcag caaacgagaa
    29041 aaagaagagt taacacgagt tgaacgtgaa gctgctaaaa caaaactccg tatagatgtc
    29101 tatcaccgtt tacgttacgt tgaaagcgat catgtcgttt ttgacccgat tacaggccgg
    29161 gaggttccgg cagaacgggc gtgtattaat aaacttattg aggcacttgc tgatgaaagc
    29221 aacaacgtga gagggtgaca gccccctgaa ggatacgatg aaatgtacag aaaaaaacat
    29281 cacctaataa aaatattttt attaaaaatg gcgtgcaatg ttttttcttt cctgctgggt
    29341 actttcatac tttcagcacc gtttgtctta tatacattaa ccggttcagt tctgaatgcc
    29401 gttattcatt tcaccttttc tttgccttta gccaccataa tcctggttta tgcttattca
    29461 ggctttgaag aaagcattta tttattcagg gcatacaaat atgactcaag caaaagctaa
    29521 tctgagaaaa cttcgccgtg cacgttatgt cccccctcga acagtaaaga aaaacatagc
    29581 gaatacagaa cgtgtcgttt caaaagcaat taccggctgt ggtgtcttcc gtgatatacc
    29641 acaagtaaaa atcaatctca tcgaaacaag gaaaatttta caaatcgaac tggctttatc
    29701 cattgctaac ttatctgagg cgaatccaat tgagatacga aaaaaaatca aaagcattga
    29761 ccgacaatta tcttcaacct caaaaatcag aactacatta tgaaaaaaaa cgacgattta
    29821 atcactttac catctcataa tagccacgct ctcgattaca tcattaaaaa agcgtttaca
    29881 ccacgcaaaa ccaaaacatc gacagcgacc cacaacgttt atagttacaa taaagaagaa
    29941 tccacattaa atttccaaaa aattggagaa gcaatttttt tcggcacaga cctggtaatt
    30001 aagctgaagc caaatattag tgcctctggt gaactcttca tctgccagaa ggactaagct
    30061 atgaacattg tttcagaatt tcttgagaaa gtaacaacca ttgttgaatc cacagcggcg
    30121 acgaacgccg ctaaggtcgc acaggcaata tctcctacat tctttgctgc tatagcctta
    30181 tacgttattt atctaatata tgaaatcacg tatgcccaac gtgacgtcct gatgagcgag
    30241 gtgactaaga atatcggtgc attcgccctg gttggtgcat tcacttattc agcgccttat
    30301 tacagccagt ttgttattcc ttttgtaatg cacgctggct ccgacctttc tgctgctgtt
    30361 actggaggtt caggaactgc aaccagtgtt gataatttgt ggaacgctct atctacgaat
    30421 ttaaacaact tccttgatac aagtatgagt atttatggaa agttccaaat tgctgagaaa
    30481 atgggggttt atctagtatg ggggattggc tatatcggcg gaatgcttct catctattac
    30541 actactgttt tcttgacact atcaactttc atggttggaa ttcttctttc cgctggcatt
    30601 ttattcattt gtttcgctct attttcatcg accagaaata tgtttactgc atgggtcggc
    30661 agttgtctca actatatact gttaaattta ttctatagca tatcatttag tttcttaatt
    30721 agtttcgttg aatcagttgt ccctgttaat ggagatataa aactatctat tgtagtttac
    30781 tttttcctta ttacgattat ttccatattc cttatagaac aagttggcac actctgttct
    30841 acattaacag gtggggtagg tataaatggc cttacatcag ccgctaatgg ctttggaggt
    30901 aaggttgcat ctggttttat gcgagcatca ggactccgat ctttcactaa tggatttgcc
    30961 agcaaaatgc caaatccaat ctttaatgca ggaagtcgtt ctgcatcagc attagttcag
    31021 ggattaaacc gtggaagtaa acttaaagcg gggtgattaa catgaaagat aaagtattat
    31081 catttctctt tgctggaata attaccgtaa catcaccatc aatctttgca tctggtattc
    31141 cagtagtaga cgttacagcg atagctaaaa cagttgaaga agggctaaat cgtgccgctg
    31201 aagcggcaag acagcttgag caactcaagc aacaatatga acagaccata agatacgctg
    31261 aagaacagaa gaaaagactc gaaggcttca ctgatttcag caatggcttt gatagcgcat
    31321 ccagctatat gaaggattct ctatcaacaa taacaaatag tgcaaaatca gatcttagct
    31381 ctttgcgatc gcaatatgac ttatcaagca acattgcaga gacgcaacaa aaatatgatg
    31441 caatcctggc taagatcaaa ttctatgaaa atttcaacaa tgaaatgcag gaaagggcta
    31501 agagattaac cacgttacag aaggagttcg cgtcagcaga tactccgcaa aaaaaagctg
    31561 acttatctaa ccaattgaat acagaaaaat tgacaatgga gctacaattg aagcaatatg
    31621 atattgcaga gagacagcta atcgcgtctg aaaatgcaag acaagaggct gttagtcgag
    31681 aaaagtttaa attaatgatg caataggtgt tcgaatgaaa aaattcataa gcactttaac
    31741 tatcttgtca tgcttccttt tagctgcttg tgaagacaaa gtttatgatg tcagttacta
    31801 cgctgaacat cttgaacaag cccaagatgt tgtcgaaaaa tgcagcaaag gggatatgtc
    31861 tggtcaaaac tgcgaaaatg cgcgtgaagc cattcaaaaa gagcaatcgg gtaaagcatt
    31921 taaaaatatg atgcaataat ccttcattaa aaacagctcg caaagcgggc tttttttggc
    31981 tgtacgcagt gaccagttga taaatagttc taatgttcgt gctgcattag aacaacgcac
    32041 aaaaagaaga gtacaagaag acagtgcggg atctaatgga acgtttacaa tagaatcacc
    32101 ttccctatag caaggtgatt ctatttaaaa gaatacccgc ctcgtgcggg ttttatctta
    32161 cttaaagatc tatgaaacct ccggcttcgg gtattaaact ataacttgct ttggcaaata
    32221 ttgtttcatt gacttcatgg aatcttattt tctccgcctg ttcatatgtt acagggaaga
    32281 acacgccccc tggctcatta aacatcataa aatcaccttg cattgcgtta caatgacaac
    32341 aatgattcgc gtaatagttc atttttgctg ttttgctatg ggatggataa tataattttg
    32401 aatactcatt aatccttgag agagcagagg ttgaaattat ttcaattttc gttaaatcag
    32461 tccaccatac tctactggtt aaccaccgat aaatcatatt attctttgaa tcaaaatact
    32521 ccataccctt aggggaggat gtttcatacc attcatctaa tatacaatct tcccattcgg
    32581 caggatcaac tccccaaata acgtctctat acttatacca acgaggtaaa aaaataccaa
    32641 acacaggagt tgttttctta caacgccaac aagactggtg actttccgca atataaaaac
    32701 gttgcgaaaa aacattgtaa tcatgttcat ttaattcttt tatccaccga ataaaataaa
    32761 ttggattttt tccctgtgga acaaaccatt tccgttgctc ttggtcccat tgcgcccctt
    32821 tttgtttagc caaatccttt tcttcaaacg gaacatttaa ataaaaccgc gccatagcca
    32881 acttaactcc agtattctgt tctgatgatg caccaggata gatatcaaaa ctaaacaaag
    32941 agtgcaattc cgcctctaat ggctcattca tacccgcctc gtgcgggttt tcttttgcct
    33001 gaaatatgga ggaactccat gaaatccatc attttatcga tggttgccgc cacagcatta
    33061 ctgtcaggct gcgcggatag caaaacacaa ccagtacctg agtcaccgtt aacctcaaat
    33121 gaccaccaga taacgttgaa aattgatacc gtgcgaacag acctgccgga cagttctcct
    33181 gtctgtttga atggtatgaa tatatacgaa agtctgagcg agcggcatcc aggcttgttt
    33241 gccaaaaatc agaacgtgat tttccgtagt gcattctttg atgacggtca ggcgatatcc
    33301 tccgaaacaa tccgcaaaat aatgcagagt accccgtgcg ttgaatcaca aacaacatct
    33361 gccggaagct gggaacctgc cacggaactg atcccccgtc aggcaatatc cggtgacact
    33421 acactggctg aaaaagatcg gactgctgac ttcacaatgc ggatagcacc ggagattgag
    33481 cgtggaaatt tccacctgct actcacaacc actgacccga tacggaaaca gactgtcgag
    33541 cagaatattt tactcaaacc ggaccagtcc cttatcgtga gtggttttac tggagttaat
    33601 gctgatagcc aaagtgagaa ccggattgtc atcattacgc cacacgtgcg ttgattcccc
    33661 tgatatttaa attacccgcc tcgtgcgggt tttcttttgt ctgaaatatg gaggaacacc
    33721 atgaaatcca ccattttatt gatggttgcc gccaccgcag cattacgacc aggctgcgca
    33781 gatagtagaa cacaccccgt gcctgagaca ccgttaagca aaatcatgtt atcgatgatt
    33841 gccgcaagtg ctatcgcact ctctgctccc gtatatgcgt ctgatccgtg tgcttccgtt
    33901 ctttgtctgt acggtaaagc tatcggtagc ggcggcggga gcgaatgcaa aagcgccgaa
    33961 aaggacttct ttaatatcat caaaaagaaa aagggatcta ttcgctggag caaaaccttt
    34021 gatgcccgaa aagcgtttct taatcaatgc acgaccgcag accctgcggc gatctcaaaa
    34081 atcatgagta agttcggacg ttcctggggt taaccatgaa aaagctcatt attattccca
    34141 ccacattatt gctgaatgcg tgttcccatg cccctgaacc cgcacaggca aaaggaggtt
    34201 ggtatgaact gaataccaca attgagcagg taagagcaga cacatactat aacggggtga
    34261 tccttcagca gcccgtgtca cagccaaaca gcactgttca atacagcaat gataattatc
    34321 tcaaaaagga agccataaac gtaactcccg cgcctcgtat tgcagaaacc gtaaaaccac
    34381 gttctgagca atcagcgaca gcgccaaaaa accagggcag caataatagt tttctgaagc
    34441 cgacaacaaa cagcataact tcatctggtg agataaaaac agcagccaaa ggagcaacta
    34501 aacagcattc tgaacaacca gcactctctc ccgcaaagcc aatcagtagc aacaactatc
    34561 aggcacaaac ggcacaaaag actgatcagc agacaaaacc gcgtcctgaa caaccaccgc
    34621 taatgtcaaa aagcggtttt ctgaaaaaaa acgctaacaa cgctgttccg gccaccacgc
    34681 ttacaggccc atcaacacct gaaaagcaaa tttactttgt tcgccatgat ggtaaaggct
    34741 cgctgcaaaa agcattaaaa gaaattgtcc catcggactg gacggctgaa atatccccgg
    34801 aggtaacgaa aactttccgt cgcacgatat cctggcaggg caacgatcaa tggccttatg
    34861 tcctggataa aatgcttcgc aactacggtc tgacggtaat taagaacacc gaaaaaagac
    34921 atcttctgat tgattttcaa caacagaaag ggaaaacatc acaaaaagca cctgtatctc
    34981 ctgacaaaac ccctgcatct cctgtcagga caccagtttc ctctgacaag ttacctcaaa
    35041 aatcgctaac gcctgtcacc gttccgcaaa aacagacaga aaaactcacc tggaatctta
    35101 aaaaaggttc gacgctgcgg gatggcctca cagaatgggc atcgagttcg ccgtgtggta
    35161 attcaacctg gaatcttgta tgggatactc ctgtgaacta cagaatagat gctcctctta
    35221 ttttccgggg aaactttgaa tctgcattaa aagatacgct gatgctttat ctttcagcag
    35281 ataaaccgtt atacgcattc aggaatattg cacaatgcgt aatcacaatt aaagacacac
    35341 aacaggagta atcgctatgg gttggttctt cagcttgctc ctgatattcc tgtcaatagg
    35401 cagttactat ctgacagata acaataacca aaccatacag cgaatgaagt tgcttcctgc
    35461 aaaacaggaa gcaattgaat ttatacattt cgcaaatgtt atcaatgact atctttataa
    35521 atatccagac aaacgtaatt caggaggaac gttaacatct gagcaaatcg gtataacacc
    35581 cgtttatgat attcatcata ttatttacgg taagcgagtt tatatctggt ctgccgatac
    35641 agaaggtctg atgtctgctt tgcagcagca aacaaaacat tcagcaatgt taggaagagt
    35701 aaaaaataag aagattgtcg ataatcaggg gaatgatatg ggcgttcccg ttccttcctc
    35761 cattccagaa gaggctattg tactcattaa ctaaatatcc cctgacaagg ggattctgaa
    35821 ctggagtctg catatccgac acatcattat gtgggccgga tatatcgcta cggcgcggac
    35881 ttcagtccag aaaatcttta ccggagatac ccatgcagga taatgatgat gtcagcgagc
    35941 ttgatattgc cactcgtgaa gtccttatct tacgatgcaa tcttattcag cttgcttctg
    36001 aatatataaa aataaatcct cttaaaaaca cccgaatcta cacagattta attcatcgtt
    36061 cagcagatat tatcaaaaat atcgaacaag gtaacatcca gaataaacat aattatctgt
    36121 cacaggatgt aaaacatacg cttgttttat acgacctgta caaatcagac tttgaacaat
    36181 gtccgggggc aaaatccctt atacctgtca gggaaaaaat tgtctcgctg aaagaacttc
    36241 tttccggcgc taatgagctg gacagtatcg ttcgtcgccc ccgcaggaaa aaacgttctt
    36301 cacgaattta atatcaggag ataaatccct tatgcagtta tcagcggctg ttctggcagc
    36361 actgataagt cagtgtgccc ctgatgtatc acccgatacg atgaatgccc tgattatgac
    36421 tgaatcaggg gctaatccat atgtaatagc gaatgtttct gacggaacaa gtaagtattt
    36481 taaagatgaa aaaggagcta ttgaatatgc agaaaaatta acagcagaaa ataaaaggtt
    36541 tagtgcaggt ctgacacaga tatacagcaa aaatttccca tcactcaatc tgacaaataa
    36601 aacagttttt gacccgtgta caaatataaa agcaggagcg gcagtcctga cggacaatta
    36661 tctgagacaa aaggagggta gtagtaatca aaaaattctc cgcgctttat ctttgtatta
    36721 ttcgggtaac gaatcaacag gatttattaa agaaaagaaa tttaacaaca cttcttatct
    36781 ggaacgggtt attcgtaacg cgaataacta tatcgtgcca tcaatccgtg aaaaaactga
    36841 tgaatcagaa ataacccccc cgaatgatga agagaaaaca tcaccggact gggatgtttt
    36901 tggagatttt agttaatgaa agtcaaatca actttgcagt attttattct ggcgtttttc
    36961 ctgtcactaa ctgcctccgt tgcctatgca ggtggactcg acaccgcaac aaataccatg
    37021 acagaactta agacatgggc atttagcttt ggtggtgttt gtgctttgtg ttacctgatc
    37081 tataacgtca tcatggcatt catggagaag aaaagctggt ctgatgtagg aatggcgctt
    37141 gtctattgtt ccctcgctgg cggtgcactt tacgccggga actgggcgct ggaactcttc
    37201 aaataagtaa tccatatata caactgtgcc gcatatttac cttatgtgtg aattgcggct
    37261 tttttcatgg tgagttatgg acaaagtaaa tgaacatgtc agtgagtcat tcatgactta
    37321 taacgggttt aaccgtcctg cgttaattgc tggtataccc ttaatgttgt tattgttcac
    37381 ggcttttttc gctgttctga caggcttccc cgctattttc ctttggggta taaaagggat
    37441 aattattcct gtcatatgtg cgctgtttct ttttatagta aaactggcct gtgaaaatga
    37501 ctcaaacgca ctaagagtca tccgtttaaa ccttatgggg cttttgttaa aaatccgcca
    37561 cagagattta attattggtt attcatctgt caggtaacat atatgcacaa aatcaaatta
    37621 aaaaacagtg acaatatgga tgttgctcgt gaatatcccg attatcgctt ccatataaca
    37681 gacagtatta tctttacttc tgacaggaaa atgctggctt cacttgttgt agcaggcatt
    37741 ccatttgaga cagagaacga taatgttctt acaaatttat ttaacagcgt taaaaacttc
    37801 ctgattgggt taggaaagga aggcgacctg tatttatgga ctcatcttat caaaaaaaga
    37861 ataaccattg atggagaatg gaattttgac aatgcttttt taagcaggtt ttcaaaaaaa
    37921 taccttgctc tgttcacatc cagcgcattc tacaaatcaa catggtattt aacatttggg
    37981 attccctacg atgatgttga taccggaatc gagcgcatga atgaaatgac acaacaggcg
    38041 caaaaagcac ttcagccatt taacgcttct gttttatcag ttcacaatac ttatattagt
    38101 gaggtggcag actatttatc actattgcta aatacagaac ataacatgat tcctttatca
    38161 tccactccct tatcatccag tatttgtgat tctgaatggt attttggtgc tgatgttctt
    38221 gaactgcgta acaacgaatc ggatagtaaa aagtttgcaa cgaattatat tttgaaggat
    38281 ttccctatag aaacgacccc cggacaatgg gattttcttc tcaagcaacc ctatgaattt
    38341 attcttactc aatcgtttat ttttgaatca cccacaaaaa ccctgaagaa cattgattcg
    38401 caattaaaca aactgcaatc agctaatgat gcagccaaaa cacaacagga agaactcgaa
    38461 gctggtaaag aagcggtcgc agccggaatt acgttgtttg gttcactaca ctgcgcatta
    38521 acggtattcg gtgatacacc agatcaggcc agaagcaatg gtataaagct ctctgctgag
    38581 tttattactt cgggtaaggg attccgcttc agtcgcgcct cactcgcatc accttttgtt
    38641 tttttcagtc atatgccgct gaataagagg cgtccactgg atacccgaag aacaataacc
    38701 aacctggcct gtctgatgtc ttttcacaat tattcctccg ggaaaaaatc aggcaatcct
    38761 attggtgatg ggtccgccat aatgccgttg aaaacggtgt ctgacagcat ttactggttc
    38821 aatacgcatt acagcccacc ggaaaaaaat gttaccggac aaaaaattgc cgggcatgga
    38881 atgattcttg gcgcaacagg cacagggaaa acaacatttg aagcagcagc cagcggtttt
    38941 cttcaacgct ttgacccttt aatgtttgtt gtggatttca accgttctac tgagcttttt
    39001 gtccgtgcat acggaggaag ctatttcacg ttgcaggaag gcatttatac cggctgcaat
    39061 ccgtggcaac tggcagaagg cccggaatct cctgtctggc acagacttct cgcatttctg
    39121 aaacgctgga cacaggttct ggcacgagac aaccagggca acccctgttc tgatgaacat
    39181 ggcattgaat taaacgctgc cgtggaaagc attatgcgaa tgcctgtcga agaacgccgg
    39241 acagcccttc ttctcgatat agtcagtcct gaacttcaga cgcgtctcgc caaatggtgc
    39301 gacaacggcg aatatgcctg ggcggtggat tccccccgga ataccttcaa tccactgcat
    39361 cataaaaaag tgggatttga tacaaccgtt gtcctggaca caaaaaacgg tattcatcct
    39421 gcctgtgaac ctttactcgc ggtcctgttt ttctacaaag aaatcatgca acgcggcggc
    39481 aatctgatgc tctccattat tgaagagttc tggatgccag caaactttcc catgacacaa
    39541 agcatgatta aaagcgcact gaaagcgggg cgcatgaaag gtgaaatgat gtggttaaca
    39601 tcacagtccc cggaagacgc cataaactgc gcaatttttg cggcgctggt acaacaaacc
    39661 gcaacgaaaa ttctcctgcc caatcccgat gcaaaatggg aaggatataa agaaattggg
    39721 cttactgaaa aagagtttga aaaactcaaa gagctgacga aggagtcaag gaccatgctc
    39781 atcaagcaat ctggctcttc tgttttcgcc aaaatggatt tgtttggatt cgatgagttt
    39841 atccccgtgc tttctggctc tgaaactggc ctgtctattt ttgatgagat tattgctgaa
    39901 aaaggagatg tagctcctga tatctggata ccagaactcc tgaaacgcct gaacggttaa
    39961 tttctctgtt ttatacagaa cggaaaacaa tatgaaaatc cccctcataa ttgcctccgc
    40021 gctattaatt agtgcatgtt ctcacccccc caatcctgtg cagaaaaaag ggggatggtt
    40081 tgagttaaac acagcaattg aacagatcaa agcaggtgat tattaatgga aaacacaaac
    40141 tattatgaac attcaaaaaa agaagccacc aaaaagaaat ttgaagaaaa aaacgaaaaa
    40201 aaggattatt tcaaggccat acgtgacttt gagcgcagtg aaattgaaat cataaagaaa
    40261 aaggcaaaaa catttaccat tctcgccatt ggtgaatttg ttgtaatttg cattcttggt
    40321 ttcgcaatag cctcccttgc tccattaaaa accgctgtac cgttccttgt gcgagtggat
    40381 aacagcactg gatatactga tattgcccct caattatctg atgctaagga aagttatcag
    40441 gatgttgaga ctaaatattt cctgtcaaaa tatctcatta actatgaagc atacgactgg
    40501 cagaccatcc aggaacaggc cgatacagta aaaacaatgt cttcccaaaa ggtgttttca
    40561 gcttatgaca ccatgattcg tgctgattcc tcaccactga acattctgaa aaacaactac
    40621 aaaattaagg tccagattaa ttcagtaatc cttctaagga aggatatggc gcaggtgcgt
    40681 ttcaaaaaaa tggtactgga tttatctggt aaacccgccc ccggatacag agcaacagag
    40741 tggatttcga cgatttcttt tgactgggat aaagatatca aaacagaaaa agagcgcctt
    40801 gtgaatccgc tgggactgca agttctgagc taccaacctg atccagaggt tataaaatga
    40861 tgcttaaacg cactactgcg atagttcttt tgtcgatgct acaagtacct ggtgctttag
    40921 cagctatgta cggcacacca tcggaacgag acgggcgtat tcagaccgta gattataacg
    40981 agcaggatgt atttaatgtg cgcgtaaaag caggcgcaca aacaacaatt aagttcggac
    41041 aagacgaaac aataaaagat gtcggaattg gtgatccaga agcctggtca gtatcggtca
    41101 gggacaacac tctgtttctg cgtccaaaag cagaagaacc ggatacaaac gttaccgttc
    41161 agaccaataa gcatatatat cctctttacc tgatatcaac cacgaaacaa cccacgtata
    41221 ttctgcgttt taattatccg aaaccaccgt cagctaccgt aatgactgaa aagccatttc
    41281 catgcactga tggtggaata attaatggtc attatcaact caagggggac aaatcaattt
    41341 tcccttatca ggtatgggat aatggcgaat ttacatgcat gagatggact aataaacagg
    41401 aaattccggt cctttatcgt gttgatgccg atggaaatga acatctggtt aacggagaca
    41461 gaaataaaaa cacaatggtt tactatgacg tggctgaaaa tctccgtttg cgcctgggtg
    41521 atcaggttgc agatatcaga acatcatcaa tcgttaatcg tccgtggaat aaaaaaggta
    41581 cgtcaaacgg aaaaacacga gtagagaagt tcagctatga aaaataaaga tgatgaaaaa
    41641 aataatgacg cgggaaatcg cggaattatc gaagtaaaag gtaaagcagc accaaaaaaa
    41701 atattaatcc tcatcatatt actgattgcc gcgttatttg tgatcattat cttgtttaaa
    41761 gttctgtcac gagaacaggt cgttcagcaa acacctcttg agaagtctga cgagacactg
    41821 gtcacaaata ccaacaatgg cgtttcactg acaacaatga tgaaaaacat cgaagaaaag
    41881 gaaaaactcg atgccgctaa ccgcaggaaa gcacaggagg agcaagaaca gaaacaggca
    41941 gacaatgccc cgtcagcgcc agcatcacaa aaggccgatc aaacagccgt caatgtgata
    42001 gccaacggca ctccccaaac cgccagtgga gatccaaacc aaccacaacc attgccaaaa
    42061 tccgtccgac aactgatggg cgacacaatg gtgaagatcg acaatcagga accaggcgaa
    42121 aaaaaccagg aaagagatga tttacagggc agtcagtacg cagacggaaa ggtatcccct
    42181 gtgctgaacc gccgatatct gttatctgcg ggaacagcat tatcctgtgt gctcaaaacg
    42241 aaaatcgtaa cctcttatcc cggtatcaca atgtgtcagt taacacgaga cgtctggtcg
    42301 gataacggcg aagtgcttct tgcccgtaaa ggagcattac tgatcgggga gcagaataaa
    42361 gtcatgacac agggagtagc ccgtgtgttc gttaactgga caacgctcaa agatgaaaat
    42421 gtcaatgtgc gcattggcgc acttggcact gacagtctgg gcgcttccgg ccttcctgca
    42481 tgggtcgata atcattttgg tcagcgtttt ggtggtgcat tgctgctgtc attacttggt
    42541 gatggtttag atattctgaa aaacagcacg caacaaacag gctcaaacag caatatcacc
    42601 tacgaaaaca catctgacgc aacaaaagaa atggcaaaaa caacgctgga caatacgata
    42661 aatattcccc ccacggcata tattaatcag gggactgtat tgtctgttat tgttccgcga
    42721 aatatcgact tttcatccgt atatgaactt cagtaattca gtaaagcaat tgatatgaat
    42781 aacatcagag ataccagtaa tacatcacaa ccacgagata acagtaaaac cgcaagacga
    42841 tatctggata tgacgggtat ccaatctgtt cttaatattg aacatgtcac agaaatatct
    42901 gtgaataaac caggaacaat atggtttgaa gggaaaaatg gctgggaatc taaagatgcg
    42961 ccagacgcga cttttgataa tttaatgaca cttgccaaaa ctctgacgaa cttatcaaag
    43021 ataaaaatac cacttagcca tgataatccc attgcatcgg tagttcttcc cggtggggaa
    43081 cgtggacaaa tcatcattcc acctgcaaca gaaaataaca gcgttgtgat atctatccgt
    43141 aagccatcat taacgcgctt tactatagat gattatgtcc gaacagggcg ttttgataat
    43201 gtcaggattg cgacgaaaca cgaagccatc ctgactgaac ggcaacgcta cctgtatgaa
    43261 ttatcccggc gtccagatgg tcaatctaaa gctcaatttt taagagaggc agttaaagac
    43321 cgtcttaatt ttttaatcgt cggtgggact ggctccggta agacaactat agcaaaggca
    43381 attgcagata tctttccacc tgaacggcgt tatataaccg ttgaagatgt accggaaatg
    43441 tctctgccgc tgcatccaaa ccacatccgg cttttctaca aaaaaaatac cgttgaagcg
    43501 aaggaaatca ttgaagcgtg tatgcgatta aagccagatc acatctttct tgctgaactt
    43561 aggggtaacg aagcatggag ttatctggag gcgcttaata cagggcatga aggctcaatc
    43621 agtaccattc atgctaataa tacttatgcc tcattttcac gactggcaag tattgtaaaa
    43681 caatctgatg ttggtatgac tgttgatatg gacctcatca tgcgcacgat aaaaacatca
    43741 attgatgtca ttcttttttt caaccatacc agaatgactg aactgtatta cgaacccgaa
    43801 gaaaaaaatc gctttttgag cacaatgtaa actctggaga taactatgga tgcaaaaaag
    43861 acgggggggt taattctctt cctgttactg cttcttgtgg gtgtgcttat agcctcaaac
    43921 tatctcggtg gatacacagc attaagatat tcatccgttg atatgtcttt gctcaagtgg
    43981 gatacatttc attctgtaat ctcaacattt tctggcaacc cacaatataa aaaactcgtt
    44041 tttatggcat ggtttggatt cagcgttcca ttaatattct ttgccatatt catgttgatt
    44101 gtcgtgattg ggattatgcc taaaaaagtc atctacggtg acgcccggct cgcaactgat
    44161 atggacctga gtaaatcagg ttttttcccg gataaaaaat caccttacaa acatccacca
    44221 atactcatcg gtaaaatgtt caaggggaga tacaaaaaac aatttattta tttcgccgga
    44281 caacaatttt taattcttta tgccccgaca cgttctggta aaggggtggg gattgttatc
    44341 cccaactgcg taaactatcc aggaagtatg gtcatactgg atatcaaact tgaaaactgg
    44401 tttttatcag ccggattcag gcaaaaagaa ttagggcaaa aatgctttct ctttgcgccc
    44461 gccggatacg cggaaactat cgaccaggca attaaaggac agatacgctc tcaccgctgg
    44521 aatcctctgg attgcgtcag tcgcagtgat ttacttcgtg aaacggacct ggcaaaaatt
    44581 gccgcaatcc ttatcccggc aagtgatgat cctatatggt ctgattctgc acgaaacctt
    44641 tttgtcgggt taggacttta cctgctggac aaggagcgtt ttcacctgga acagaaagcc
    44701 aaaggtcata atgtaccgga cgttcttgtt tccatcagcg ccatactgaa aacgtcggtt
    44761 cccgatggcg gcaaggatct cgccgcatgg atgggacagg aaattgaaaa ccgtagctgg
    44821 attagcgata aaacaaaatc attcttcttt aagtttatgt cagcaccaga tcgcacaagg
    44881 ggaagtatag aaacaaactt ttcatcaccg ctgagcatct tcagcaaccc tattactgct
    44941 gaggcgacga atttcagtga ttttgatatc cgggatattc gtaaaaagcc catgtcaatt
    45001 tatcttggtc tgacaccgga tgctttaatc actcatgaga aaattgtaaa ccttttcttc
    45061 tcattactgg ttaatgaaaa ctgtcgtgaa ctgccggaac acaacccgga cctgaaatac
    45121 cagtgtctta ttctcctgga tgaatttaca tcaatgggta aatcggaggt cattgaacgc
    45181 gctgttggtt ttacagcagg ttataacctt cgctttatgt tcattcttca gaatgaagga
    45241 caagggcaaa aaagcgatat gtacggtcag gaaggatgga ccacattcac tgaaaacagt
    45301 gctgttgtgc tttactatcc gccgaaatca aaaaatgctc tggctaaaaa gatttcagaa
    45361 gagattgggg tgcgtgatat gaaaatatcc aaacgctcaa tatccagtgg tggtggtaag
    45421 ggaggaagtt cacgtacacg gaatgatgat gtaatagagc gcccggttct tcttcctgaa
    45481 gaaattgtgt ctctccggga taagaagaat aaagccagaa atattgcaat ccgtgaaatt
    45541 ataaccagtg aatttagccg accatttatt gccaacaaaa taatctggtt tgaagaacca
    45601 gagtttaaac gccgcgtgga tatcgcccgt aacaatcatg ttgacattcc aaacctgttt
    45661 acacaagaag tcatggatga aatagcaaaa attgcagaaa tttatctccc taaagctgga
    45721 ggaaaaaaag tcatggttgc cggaggcaat gtgattacta atccagacct tgataatcac
    45781 gataaaacag acgtatctga ataatcctcg caaccagcaa atagtatttt gcatattcca
    45841 taaggtaatt aaacatgaat aaaaaattgc tttgtttatt aatatcctct tttttattat
    45901 caggctgtgc aaacttcaaa aaaatcaatg ctaatgaact tgctgccgaa agagacgcac
    45961 aaaaagcgac tgaatacgcg aaaaaaattc gtaataaccc ggttatccag gattcaacaa
    46021 aacaatggat taatacaacg ccaattattg aaaagcaggt aaagcaatca gcgccgccct
    46081 gcccgattgt gattaatact cgttcagata ttgagctacg ccagatcgca caacgcatca
    46141 cacaaacctg tcatatcccg gtacgaatca gcgccgatgt ctggacctat ctgaacggta
    46201 caggcactgg ctcaacacaa caaatgacag gctcaattcc agcacctgat gcaaatggaa
    46261 tggttcctct ggcgtcagta ggcagtcagc cagtaaaagt ctcttctggc ggtacatcac
    46321 tccctgaact gaatatctcc gggcttcctg ccctactgca aaccgtatct tcacggctgg
    46381 gaatatcctg gaaatacgat aaaggtcgta tcacattctt ctatctggaa acgcgctcat
    46441 tcccgattac atatatggac agcaatgtgg catacaactc aaaagttgtg agcggcacaa
    46501 tgtcttccag tggcagtacc ggctctacaa gttctggcgg tatgactggc gacgcatcaa
    46561 atacgcaaac tacaaccgtg gaaatgaaat catcgctgta caacgacctg aaaagcgaag
    46621 tttcctcaat gctgacacca ggtacagggc gcatgtatct gtccacaggt tcactgacag
    46681 taacagatac cccggatgta ctggacagcg ttcaggagat tgtgaacaag cgcaataatg
    46741 aaatgagtcg tcaggttgtg ctgaacgttg aaatcctgag tatcaagaaa tcaagccagg
    46801 aacaggcggg aatcgactgg aacgcagtat ttaatgatgg tcatcttggt ctgagtcttg
    46861 gtggctcatt tggtgatgcc gccgagaatg ttattaccag tggtgtatcc atcgtggacg
    46921 gtaagctggc tggctcaaaa gcatttctga aagccctgtc gtcacaaggc agcgtcagtg
    46981 tcgtaacgca aaactccgct gtgactaaaa acctgacgcc tgtccctatg caaattgcca
    47041 atcagcaggg cttcattgaa agtgtcacaa ccgattctac cgccaatgtg ggatcatcca
    47101 cgtcactgaa tgcggcaaca ataacaaccg gattcaatat gactctgctt ccatacattc
    47161 agccggattc acaaaacctg caattgctgt tctcaatgag tctgtctgac aaacctactt
    47221 ttgaggtgtt cgaaagtggc ggcagtaaag cacaaacacc gaatgtggat ttgaaaacaa
    47281 ttaatcagac tattgatctg aaatcaggcc agacagtcat tctgagtgga ttccagcaat
    47341 caaaccggaa aaccagtaag caaggtgtaa taaccccctc cttctttgga cttgggggtg
    47401 gcgttaacag cgaagacggc gacacgattc tggtcgtttt gattacacca aatgtgctta
    47461 attccggctc cagtgcagta ataaaaaatg tgaactaatt aactgaaaac tccctgaaaa
    47521 aacacaggga gtttttttgg aggtcacttt gtctgagaat aatcagaata accggaattt
    47581 cacttcggtt attaaaaaca agcgtgcttt tttctccggt ctggactgga aaacgcttcc
    47641 ttcagaagaa aagaatgcgc gtacattcgc aagaaaaaac gacgctgaat attttctctc
    47701 ctgtcagtat caggattcag aaaatgaaac caaaacgatg gtggcattta tcagaaaaga
    47761 ggatcttccc acaggggcgt cctcattctg gtcactggca ctgatgataa aacccctcat
    47821 cgaacctgac ggatacgcta tctgcgaact cggtgatctt tatggctttg tttcctgcgt
    47881 caataacgtt ctcgtcaacg atgttgtagg taacaaaagt cagatcatga gtgctctgac
    47941 aacatttctt gagtttaatg aaacaccaga acccggctgg aaactatacc agcctgaatc
    48001 atgggatatc agccaggcac tcccctcact cactctgtct gcgttgattg acgtaaaaaa
    48061 accgccgaaa gaggcggcgt ttactcgcgt atcccgaaaa cgtcagttca tgatttatgg
    48121 cggaagtgcc attctggcca tactgctctg gaacgggatc accatgtacc aggaataccg
    48181 ggaaaaagaa gctgcggcag aagctgcacg tcttcgtctg gcaaaagaaa tggcagacaa
    48241 acaggcaata cagattgccc caccgtggca acatttacct gaaataaaac catttattga
    48301 taaatgcatt gataaatggg atgcactgcc cttatcgatc gccggatggc gttttgacct
    48361 tgctgaatgc agcacatccg gtaatgacgg gctgttacgc acatcatata aggaactgag
    48421 cggcgttacg gtcgaggact tcagcacacg gatacgagaa atattccagg ggacaaccac
    48481 agcaactttt gtattgccag aaggcagcgc cgggggattt tctcttcctg tcagttttga
    48541 cgtttcgcct gatccaatta ccccggacac attgccacag gcaacagata ttcaggaacg
    48601 cctgacaaca tttgcacaaa aaatgaggtt aaaactcacc tggcaggaaa ttgaaaacac
    48661 aaaaacagat gaagaaggcc gtccaataat tctcccgtgg aacgagtatg aattaatgat
    48721 tcagaccagc accccgcctt caatactttt cgctaacttt catgaacctg ccgttcgctt
    48781 ccagtatgca ggaataaaac tggaagaagg aagactgaat tatgagatta aaggtgcatt
    48841 ctatgttaaa aataattaaa ccaacgatta tttctgtctg ttttttcagt tcacttgctt
    48901 acgcaacaga atctgaaagc acaccaaaag acgaaataaa atattcatct ccgaccattt
    48961 tacaggtaga gcagattcag gctgaaacgg taatttacga agcaaagctg gcgcgtcaga
    49021 aggctgtcag tgaattgcgt aatcttgatt tgaatggtgt agcgtccgca acgccagcaa
    49081 taacgccctc ttccgggtat tcttctcttg cagataccga tataccagcg caacgtgtag
    49141 ccgctaaatc acttcgtgtt attgaaatat ttggtacacc acaacaaatg tcagcacgaa
    49201 tttctctcct ggacgggtca gtaactgatg tcaaggtcgg gcaaagtatt cctggtactt
    49261 cttataaggt ggcatcaatc actggtaatg gcgtctggat tcagaatggt acagagaaac
    49321 gccagcttat tctttaatgc tattgaaaga gacgacacat aatgtttttt tctgacgcag
    49381 actatcccgg aaaagatatt tctgatgttg tatacgtcag tatatctgaa aaagacgata
    49441 aacctgttca ggtttatatc gctgataaca gtaaaggaca gcgtgctgtt caggcttata
    49501 ttggcactct gaatgttaaa tatcccggac aggtaaatat aacctcgaca actcttgatg
    49561 ttatcgcatc ccgctaccag gacagcgaaa aaaggtctga ttcaaactca tcacaacact
    49621 taaacagcaa ccaggaaaag gtcattagct acctggcgaa agcaaacaaa ctgggaagtt
    49681 ccgatttgca catcacacct ggtcgggacg gctccgagtt cacttatgtc gaagcacgcg
    49741 tgcatgggga actggaaatt ctggatgtca tcccgcgtaa agaagggctg gaattgctgg
    49801 gtgctgccta ttctggaatg agcgatgtta tcaaaggtac gcagtttgac ccggcaatac
    49861 cgcaggatgc acgtatcgca gagaattttc tgaagcctgt aaatcttttt ggggcgcgct
    49921 attcgcatta tccgtgcgtg ggtggtgtgt acgcggtctt ccgcctgatt aaagatgaca
    49981 gcgaggatat tcccactttc gaagaacttg gctacatgcc acaacaaatc cagacaatca
    50041 ggcgaatgtt acaacgcccg gaaggaatta tcgttttatc cggccctaca gggtccggta
    50101 agtcaacgac tctgagaacg gcttcagaag cctatctgag cacgtttggt ttcaaccata
    50161 acgataatat gcgtctcccc cgaaaacgcc ttttcaccat tgaatcaccg ccggagggcc
    50221 gcattcccgg cgcaatacag acggcagtca gggattccgt cgatggctgg gttgacgcga
    50281 ttaaatccgc tatgcgactt gatccagacg cgatactgaa cggcgaaatt cgtgatcacg
    50341 catcggcatt agcagcaatc aaagcctcga tgacaggcca ccttctgttg acgaccttac
    50401 acgccaacga tgccctgaat attattgagc gccttgaaat ggagaatatt caggccaggc
    50461 tgattgcaga tcctcttctg tttatcggtc tgctgagtca acggctggta caaaaactct
    50521 gtccttactg taagcgcact tatgctgaaa tggcagacaa actgagtaca gaagaacgga
    50581 aactcattga ggaaaattgc ctgccggaac aggtccggtt gcgcaatctt gacggttgcg
    50641 agcactgtta taaaggcgta acgggaagga cggttatagc agaagtcgtc tcacccgacg
    50701 cacgtttttt tcagttatat cgtcagtccg ggcgtctgga agcaaaatca tactggcact
    50761 atgaacttgg aggaattacc cgtaatcagc acttattaca cctgattaat catggtctgg
    50821 tagatccgct gtccgcacac ttcatatccc ccattgacga agataaatac tcaatgctcc
    50881 cggaggggac atggcgataa aagatatatt tgagaaactc aattcgatag aaattgattt
    50941 ctctggaata tcccactggc tggcaaaata cacattcagg tattccagaa taagtttata
    51001 tgaagattta tctggaatgt taaatgatgg cataaatgta aagcgtgctc ttacacacct
    51061 gattgatgta gaaacagact acggaaagaa aacggggtct tctgcaacct actatatttg
    51121 ttgtgagtgc cttaatgcac tgaataacac aggggcaata tcttcagcat taaaaaaata
    51181 tataaaggag gatgaatatc aattaatatc cagcggtgaa attcgcggcg accttgccgg
    51241 gggattagtt caggccataa aaattgtcag aagtcgttct gaaatgacaa tgactcttgt
    51301 tatgtcgatg atttatccat ccgtactggt ttttggttgt ctggcaaaca tgtatatggt
    51361 ccacgatacc ataacacctg ttttcctttc tctcgcaccg aaagaacgct ggacaagttc
    51421 tatgcgatta ctgacagata cgtctggttt cctgatagaa aacggaacgt atattctctg
    51481 cttctgcatc gtactgtttt tcctgatcag atattcgctt gcacgttata caggaccggg
    51541 ccgtcagtat cttgattaca ttcccccctg gtcactttat aaaacattta atggtgtaag
    51601 ttttctcttc aacatggcct ccatgttaag tattgatatt cctgttgcaa aagcactgga
    51661 aaggctggag aaaaacagca ctcaaaacag atggctactc gaacgaataa aagaaatcag
    51721 gagatatgtt ttacttggac agtcacttgc aatggcaatg aaaagttgcg gttatgactt
    51781 cccatcgaaa ttatgcatta acaaactatt acttcattct gaaaatgctg acaatgcatt
    51841 gatgatgtct aactatgctg ataaatggct ggaggaagca aaaggaaaag tcaaatcaac
    51901 aggagtctgg ataactgtag tatccgcatt aattgttttc gcatttattg taatgatgat
    51961 tactgctctc tacgatgtca gtaacgtcct gcaacactaa tccctaatgt ttttaacctc
    52021 tcaataaata aggaattatt tattatgtct tctattaata ttttaaatat gcgttctgtt
    52081 ttttcttctc tttctgcaca ccgtaaaaaa gaacaggaca aaggggcaac actgatggaa
    52141 gtgttattgg ttgttggtgt tattgttgta cttgcagcaa gtgcatataa actctattca
    52201 atggtgcaat ccaacattca atcatccaat gagcaaaaca atgtcctgac agtgattgcc
    52261 aacatgaaat cactgaaatt ccaggggcgt tatactgaca gtaactatat taagactctc
    52321 tacgcgcaag gattactgcc atccgatatg atcgcagata caactggcgc aagcgcgaaa
    52381 aacccgtggg gcggctccgt tacaatcaca acctcttcgg ataaatactc cttcaatgtt
    52441 gttgaggcca gcgtaccgaa gaaaaactgc atggcaatgg taaacgccct gcgttcttcc
    52501 agcgcaatct ccaagattaa caacacttcc acatcaactg tcgatgcggc aactgtttgc
    52561 tcatctgaca gcaatacgct gaccttcagc actgattcct gatttttcat gtgcatcgga
    52621 taagcgcctt cgggcgctta ttttttccat gtttacagga ggagtattgt gcgttctgtt
    52681 ctttatatcc tgctatcatg tatttcatca ttaatctttt cattgcaacc cgcatattca
    52741 gcgacaacga cccgatttga tgcctgcttt gcacaagccg ggcaacgtta tctgattgat
    52801 cccctgcttc tgaaaagcat tgccactgta gaaagttcac tcataccaga ccggataaat
    52861 cacaacaaag aaaaaggaaa aatattaagc accgattacg gactgatgca aattaattca
    52921 tcacacatcc ccaacctgaa aaaacttggc gtaataaaag acaaatcaga gcttattgat
    52981 aacccctgtc tcaatataca aattggcgcg tggattctgg caacacattt tcagaaatgt
    53041 ggcataaact ggagttgtct tggcagttat aacgctggtt tcaaagaaag taacgaacag
    53101 aaaagaatca aatacgcacg ctatgtctac aataaataca tggtaaggta acttatgcta
    53161 aacagcatat ttaatacggc gatactttgc tgtgcaaata tcgtctgtca atgctacgcc
    53221 tacaatgaag taaaattccg tttaaataaa cttaatgtat cttataaaac cggcacagag
    53281 aaatattact gtctgatact gacaacgctt gccgttctgt atctttcatt aaaccagcta
    53341 tcactgattc aatccataat agtgattgtt ttctatgctt tccttacatt aatggcctgt
    53401 atagaccttc tttcatttct actaccacgc ctttataccg tgacatttat cttctccggc
    53461 ctgttatatc agacatggaa taacaatata ttgtctggtt tgttttgtgc aatgctgatg
    53521 ttcttcatta tgctgtttgt aagactttac tttgcatata aaaacggaac ggaaagtttc
    53581 ggcatgggag atgtgctact tattgcagga actggcgtat ggtttccaac gcctgagatt
    53641 gcctgttcaa ttgtatttat tgccgttatc ggcggaatta ttttcttcac tcttggcgga
    53701 ttaaacaagc aaaaaaaata tatcccattc ggtccgttcc tttgtggcgg aatgtttgtt
    53761 tactcactcg taccgggaat attattctga ggattaagag caatgaaaaa gacagataaa
    53821 ggtgtatctc tccttgaggt attacttgtc atcggcataa tggtgatggt tatccctaaa
    53881 gtttatgaaa acattgaaaa ccatctgaat aatgttcgct ggcagaatgc agcagaacac
    53941 gcaaatacct ataacaccgc tgttcgtaat tatgttgctg acaatgcatc aacactactt
    54001 gctggctctt tacccaaaac aatcacgcca gcaacactca ttcagaaagg ctatctgaaa
    54061 tctggttttt ctgaatccaa ttttggacaa agctacatta ccggtattgc aaagaacagc
    54121 aaaacatccc gtctggaagc cctgacctgt agtaatggcg gtcagtccct gtcagaagcc
    54181 ggaatgcgct cggtagcaag catgattgaa gggcttggcg gctacatcaa cagcagcaag
    54241 caggcgattg gcgcgggtgg cggctggtct gacacgcctt caaactatgg gctgaactgc
    54301 gcgaccgggc atattgcaat ggcacttgtt ggcgctgatt tgcaggaaag cgaccgtttg
    54361 tatcgttact ccataactaa tcgtcccgac cttaacagga tgcatacggc gattgatatg
    54421 aacagcaaca atctgaacaa cgtcggcact ttaaacggca acgccgccgc gctctccggc
    54481 gatatcagcg cacgtaacgg tacgttctct ggcgcaattt caggaaatac agccacaacc
    54541 aacggtgata tcacttcgaa taatggctgg ctggtcacaa aaaacagcaa aggctggatg
    54601 aacagcacct atggtggcgg ctggtatatg tccgattcat cctggttgcg cagcgtgaat
    54661 aataaaggga tttacactgg cggccaggta aaaggtggaa ctgtacgcgc tgatggacgc
    54721 ctctatacag gcgagtattt acagcttgaa aaaacagcaa cggctggcgc ttcctgctcc
    54781 cctaacgggc ttgtaggccg cgacagtaca ggggcg
//
LOCUS       DAZRVY010000016        51301 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZRVY010000016 DAZRVY010000000
VERSION     DAZRVY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.016"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHXOX_000966"
     CDS             complement(<1..1334)
                     /locus_tag="ACHXOX_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR7004509.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHXOX_000967"
     CDS             complement(1380..2138)
                     /locus_tag="ACHXOX_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004510.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHXOX_000968"
     CDS             complement(2170..2883)
                     /locus_tag="ACHXOX_000968"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR7004511.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHXOX_000969"
     CDS             3057..3749
                     /locus_tag="ACHXOX_000969"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR7004512.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHXOX_000970"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHXOX_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR7004513.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(5585..7444)
                     /gene="gss"
                     /locus_tag="ACHXOX_000971"
     CDS             complement(5585..7444)
                     /gene="gss"
                     /locus_tag="ACHXOX_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR7004514.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            7649..8515
                     /gene="yghU"
                     /locus_tag="ACHXOX_000972"
     CDS             7649..8515
                     /gene="yghU"
                     /locus_tag="ACHXOX_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR7004515.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(8544..8625)
                     /locus_tag="ACHXOX_000973"
                     /pseudo
     CDS             complement(8544..8625)
                     /locus_tag="ACHXOX_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="ACHXOX_000974"
     CDS             complement(8638..8886)
                     /gene="hybG"
                     /locus_tag="ACHXOX_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR7004516.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="ACHXOX_000975"
     CDS             complement(8899..9240)
                     /gene="hypA"
                     /locus_tag="ACHXOX_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR7004517.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="ACHXOX_000976"
     CDS             complement(9233..9721)
                     /gene="hybE"
                     /locus_tag="ACHXOX_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR7004518.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(9714..10208)
                     /locus_tag="ACHXOX_000977"
     CDS             complement(9714..10208)
                     /locus_tag="ACHXOX_000977"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR7004519.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="ACHXOX_000978"
     CDS             complement(10208..11911)
                     /gene="hybC"
                     /locus_tag="ACHXOX_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR7004520.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="ACHXOX_000979"
     CDS             complement(11908..13086)
                     /gene="hybB"
                     /locus_tag="ACHXOX_000979"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR7004521.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="ACHXOX_000980"
     CDS             complement(13076..14062)
                     /gene="hybA"
                     /locus_tag="ACHXOX_000980"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR7004522.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="ACHXOX_000981"
     CDS             complement(14065..15183)
                     /gene="hybO"
                     /locus_tag="ACHXOX_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR7004523.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(15372..15659)
                     /gene="yghW"
                     /locus_tag="ACHXOX_000982"
     CDS             complement(15372..15659)
                     /gene="yghW"
                     /locus_tag="ACHXOX_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR7004524.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(15778..16665)
                     /gene="yghX"
                     /locus_tag="ACHXOX_000983"
     CDS             complement(15778..16665)
                     /gene="yghX"
                     /locus_tag="ACHXOX_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR7004525.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            16822..17862
                     /gene="gpr"
                     /locus_tag="ACHXOX_000984"
     CDS             16822..17862
                     /gene="gpr"
                     /locus_tag="ACHXOX_000984"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR7004526.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(17902..18396)
                     /locus_tag="ACHXOX_000985"
     CDS             complement(17902..18396)
                     /locus_tag="ACHXOX_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR7004527.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            18587..19471
                     /gene="yghA"
                     /locus_tag="ACHXOX_000986"
     CDS             18587..19471
                     /gene="yghA"
                     /locus_tag="ACHXOX_000986"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR7004528.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="ACHXOX_000987"
     CDS             complement(19631..20056)
                     /gene="exbD"
                     /locus_tag="ACHXOX_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR7004529.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="ACHXOX_000988"
     CDS             complement(20063..20797)
                     /gene="exbB"
                     /locus_tag="ACHXOX_000988"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR7004530.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            21049..22236
                     /gene="metC"
                     /locus_tag="ACHXOX_000989"
     CDS             21049..22236
                     /gene="metC"
                     /locus_tag="ACHXOX_000989"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR7004531.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            22376..23035
                     /gene="yghB"
                     /locus_tag="ACHXOX_000990"
     CDS             22376..23035
                     /gene="yghB"
                     /locus_tag="ACHXOX_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR7004532.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="ACHXOX_000991"
     CDS             complement(23075..23974)
                     /gene="yqhC"
                     /locus_tag="ACHXOX_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR7004533.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            24168..25331
                     /gene="yqhD"
                     /locus_tag="ACHXOX_000992"
     CDS             24168..25331
                     /gene="yqhD"
                     /locus_tag="ACHXOX_000992"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR7004534.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            25436..26263
                     /gene="dkgA"
                     /locus_tag="ACHXOX_000993"
     CDS             25436..26263
                     /gene="dkgA"
                     /locus_tag="ACHXOX_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR7004535.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            26463..27389
                     /locus_tag="ACHXOX_000994"
     CDS             26463..27389
                     /locus_tag="ACHXOX_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR7004536.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            27440..27697
                     /gene="yqhH"
                     /locus_tag="ACHXOX_000995"
     CDS             27440..27697
                     /gene="yqhH"
                     /locus_tag="ACHXOX_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR7004537.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(27740..29959)
                     /locus_tag="ACHXOX_000996"
     CDS             complement(27740..29959)
                     /locus_tag="ACHXOX_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR7004538.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="ACHXOX_000997"
     CDS             complement(30070..31482)
                     /gene="ftsP"
                     /locus_tag="ACHXOX_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR7004539.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="ACHXOX_000998"
                     /gene_synonym="parF"
     CDS             complement(31557..32294)
                     /gene="plsC"
                     /locus_tag="ACHXOX_000998"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR7004540.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(32527..34785)
                     /gene="parC"
                     /locus_tag="ACHXOX_000999"
     CDS             complement(32527..34785)
                     /gene="parC"
                     /locus_tag="ACHXOX_000999"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR7004541.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(35331..35813)
                     /locus_tag="ACHXOX_001000"
     CDS             complement(35331..35813)
                     /locus_tag="ACHXOX_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR7004542.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="ACHXOX_001001"
     CDS             complement(35866..36258)
                     /gene="ygiW"
                     /locus_tag="ACHXOX_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR7004543.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            36410..37069
                     /gene="qseB"
                     /locus_tag="ACHXOX_001002"
     CDS             36410..37069
                     /gene="qseB"
                     /locus_tag="ACHXOX_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR7004544.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            37066..38415
                     /gene="qseC"
                     /locus_tag="ACHXOX_001003"
     CDS             37066..38415
                     /gene="qseC"
                     /locus_tag="ACHXOX_001003"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR7004545.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            38525..39106
                     /gene="mdaB"
                     /locus_tag="ACHXOX_001004"
     CDS             38525..39106
                     /gene="mdaB"
                     /locus_tag="ACHXOX_001004"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR7004546.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            39137..39451
                     /locus_tag="ACHXOX_001005"
     CDS             39137..39451
                     /locus_tag="ACHXOX_001005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR7004547.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(39499..41391)
                     /gene="parE"
                     /locus_tag="ACHXOX_001006"
     CDS             complement(39499..41391)
                     /gene="parE"
                     /locus_tag="ACHXOX_001006"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR7004548.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="ACHXOX_001007"
     CDS             complement(41420..42001)
                     /gene="yqiA"
                     /locus_tag="ACHXOX_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR7004549.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="ACHXOX_001008"
     CDS             complement(42001..42828)
                     /gene="cpdA"
                     /locus_tag="ACHXOX_001008"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR7004550.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(42853..43275)
                     /locus_tag="ACHXOX_001009"
     CDS             complement(42853..43275)
                     /locus_tag="ACHXOX_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR7004551.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="ACHXOX_001010"
     CDS             complement(43276..43905)
                     /gene="nudF"
                     /locus_tag="ACHXOX_001010"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR7004552.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            44110..45591
                     /gene="tolC"
                     /locus_tag="ACHXOX_001011"
     CDS             44110..45591
                     /gene="tolC"
                     /locus_tag="ACHXOX_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR7004553.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            45739..46410
                     /locus_tag="ACHXOX_001012"
     CDS             45739..46410
                     /locus_tag="ACHXOX_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR7004554.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            46416..47576
                     /locus_tag="ACHXOX_001013"
     CDS             46416..47576
                     /locus_tag="ACHXOX_001013"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR7004555.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="ACHXOX_001014"
     CDS             complement(47614..48402)
                     /gene="ygiD"
                     /locus_tag="ACHXOX_001014"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR7004556.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            48545..49318
                     /gene="zupT"
                     /locus_tag="ACHXOX_001015"
     CDS             48545..49318
                     /gene="zupT"
                     /locus_tag="ACHXOX_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR7004557.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(49376..49546)
                     /gene="yqiD"
                     /locus_tag="ACHXOX_001016"
     CDS             complement(49376..49546)
                     /gene="yqiD"
                     /locus_tag="ACHXOX_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR7004558.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="ACHXOX_001017"
     CDS             complement(49807..50460)
                     /gene="ribB"
                     /locus_tag="ACHXOX_001017"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR7004559.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(50564..50712)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            50834..51124
                     /gene="ubiK"
                     /locus_tag="ACHXOX_001018"
     CDS             50834..51124
                     /gene="ubiK"
                     /locus_tag="ACHXOX_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR7004560.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        12799 a        13698 c        12798 g        12006 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtaccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaaacaca
     5461 ggtattccac tggttaatat attcacatat gaaataaata ttagaacata taaatattga
     5521 atattttgat taatccccgt actgattatt cttcataatc agtacggggt tgctacaaca
     5581 tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg atgaccagtg
     5641 attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag gtacatacct
     5701 gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg tttttctgct
     5761 cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc aggtcgatat
     5821 tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc aattcatcat
     5881 taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac cagaggatcg
     5941 gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact agcacttccg
     6001 ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta cggattggca
     6061 ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt tcccacgccc
     6121 aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc ccggcagcat
     6181 cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc tggtgcagcg
     6241 cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc atgatatgga
     6301 caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc agcccttccg
     6361 ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg atcaagcccg
     6421 cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg ccacgctcat
     6481 ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc caggagagac
     6541 gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg tcatctttca
     6601 gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct ttaattagct
     6661 cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg atcacctgac
     6721 cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat ttaccgtcaa
     6781 actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct gcaatttccg
     6841 gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga atggtggtgt
     6901 catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc agctcacgcg
     6961 tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca atacggactt
     7021 tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat tcaccgcctt
     7081 tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta ggaaatgcct
     7141 gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag aaaatctccc
     7201 acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa cggcgagcaa
     7261 attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta cggaatacgg
     7321 cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag attgctaccc
     7381 cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc gttcctttgc
     7441 tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg tgctctgatt
     7501 acctgctcag cgacattaac cggacagagg tcagactaat catacacctc atcgcagcgg
     7561 ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct acactgcttc
     7621 aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc ccgcgaaagt
     7681 ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc cggtttctgg
     7741 tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt attcgctggg
     7801 aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc tgggcgttac
     7861 tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat tctccagcgg
     7921 ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata cgcataatcc
     7981 gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga aatttggcta
     8041 cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt tctggttaca
     8101 gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg cgccgatgaa
     8161 aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg acgtgctgga
     8221 taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg cggatatggc
     8281 gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg cagagtttct
     8341 tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg aacgtccggc
     8401 ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc agttgcatga
     8461 gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg ggtaagggtt
     8521 ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca tccggcaatc
     8581 gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag ctaatcatca
     8641 cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt tggcttcatc
     8701 ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc ccagtagatc
     8761 ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac cacatacttc
     8821 aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg gaacgccaat
     8881 acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg aatcgccggt
     8941 atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct gatgaatctc
     9001 caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga tatgtaaatc
     9061 gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga cggcgctctc
     9121 ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct taacatcgtg
     9181 ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca actcatgcat
     9241 tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt acatccggat
     9301 tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt tggccctctt
     9361 caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat tgcgaaacac
     9421 cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga ccaatttttt
     9481 cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact gcactcagca
     9541 tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa tcagaaacat
     9601 acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg gcaatttctt
     9661 caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc tcttcagtca
     9721 tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc cagaacctgc
     9781 tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg tggctccagc
     9841 gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt gaagcgcagg
     9901 gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt aaacaacgcc
     9961 ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt cgacacaatg
    10021 gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc cattcccgcc
    10081 gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc taacgcttcg
    10141 acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc taagactaaa
    10201 atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc actacgtgta
    10261 ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc agcggtttat
    10321 tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg tcatcattga
    10381 agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta ctgataatac
    10441 cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg aagccaacac
    10501 ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga tcgcctttgc
    10561 cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat tcgcagcagt
    10621 gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact tccagcgtgt
    10681 tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg gtagattcgc
    10741 gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc gttttgccgt
    10801 agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca tcataagccg
    10861 gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag gagtgcttcg
    10921 cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc ggacgatacg
    10981 aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg tttttactgt
    11041 cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc gtcagccatt
    11101 ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca acaaagtcgc
    11161 tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc acgcccaaac
    11221 cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg tgcggcgttt
    11281 taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc gcctgcaggt
    11341 agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg tgacccccgt
    11401 agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc ttgttctgaa
    11461 ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc agcatttcgg
    11521 aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc agcgccgaaa
    11581 gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg atgttacgga
    11641 tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca cgaacggaag
    11701 acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc catgcatcgc
    11761 gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg gaagcccatg
    11821 ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc tcaatacggg
    11881 ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac gattatgatc
    11941 gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt aagcgcaaat
    12001 ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga agtaggcgta
    12061 accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg cacaacctaa
    12121 cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct tcgccacacg
    12181 cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca cggagtagaa
    12241 gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa agcgcagcac
    12301 gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca gactcttttc
    12361 atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga tgacaatcga
    12421 gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat agctctgcca
    12481 caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag actggtgcat
    12541 ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca gacgcttgag
    12601 agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg ccatcacgcc
    12661 gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt tgaagtgacc
    12721 cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga tcgacaagcc
    12781 acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg gatggtattg
    12841 cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg caaagccggt
    12901 gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt tcaggtcaga
    12961 gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga tgacgattaa
    13021 cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt gtggatcatg
    13081 actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga accagcacgg
    13141 ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg tgttgaatat
    13201 tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga ttttcataag
    13261 gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg tacagatgcg
    13321 gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc tgacgcggat
    13381 agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc atcagctctt
    13441 cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca ggcagaccgc
    13501 ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc agcgcaccaa
    13561 acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg cagtaacggc
    13621 aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct tttttcagtg
    13681 cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac tgtttcttaa
    13741 tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg gtccacacct
    13801 gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc tgttcccctt
    13861 gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc tggcagccta
    13921 cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg cggttttcag
    13981 cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag gaggctgctt
    14041 taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat ctttcttttg
    14101 ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc caaccacacc
    14161 gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt taacatccgg
    14221 tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc ctttatggaa
    14281 gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg gccacacgcc
    14341 gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc ctttacagcc
    14401 gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt tggcaaaacg
    14461 accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc cataggcgaa
    14521 ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga tgtgcgcaac
    14581 ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa cggttttgcc
    14641 tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg cggcaacacc
    14701 gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg ccgctttgcg
    14761 gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat cttttaatgg
    14821 gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg cgttatgttt
    14881 gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact ccagagagat
    14941 agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag attccgtaca
    15001 accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt tagtaaccga
    15061 ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta atgctgcaca
    15121 aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt tatctccagt
    15181 catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc gcaaaatcaa
    15241 taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat catgatactg
    15301 cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag caagataata
    15361 cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa cgtagaacag
    15421 gaattattct cacgaaagaa atccattacc atctctttat ccagtccata gcggcgcgtc
    15481 agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta catccggtgt
    15541 gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac attaaccgca
    15601 ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg gttattcatc
    15661 gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa gaacgacttt
    15721 tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat aaacctatca
    15781 ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg cggcagattt
    15841 gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat agatatacgc
    15901 ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc cctcgttgat
    15961 tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg ccacatcggc
    16021 agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat acgcgacagc
    16081 cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt tgcctgccgc
    16141 ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca gtttggttgg
    16201 atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac ctccaacgga
    16261 acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc gtgccacatc
    16321 ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg gcgttttgcc
    16381 gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat ttggcgaagg
    16441 atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag tcgccagcgc
    16501 ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca ccgcatactt
    16561 cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat agtcgtagta
    16621 atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg cactcctcag
    16681 ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc gtcactctgt
    16741 gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg tttcgaaatt
    16801 gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta cgggcagatg
    16861 caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct cggtttatgg
    16921 cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg taaagcgttt
    16981 gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc aggaagcgca
    17041 gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga tgaactgatt
    17101 atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg cggttcacgt
    17161 aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat
    17221 atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg
    17281 gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta ctcgccagag
    17341 cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt aattcatcaa
    17401 ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga taccctgcaa
    17461 aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct gaccggaaaa
    17521 tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa agttcgtggt
    17581 ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg
    17641 gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct gaaagatgat
    17701 cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga gaacgtgcag
    17761 gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca gcatatcgcc
    17821 gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa taacgggcgc
    17881 gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa gatagcccat
    17941 cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca gtttgttatc
    18001 cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa ttgccacaat
    18061 cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc agttcagctt
    18121 ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg aaaacatcac
    18181 catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca cgaggatcaa
    18241 atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct ggaagaactt
    18301 cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa gcagccagcg
    18361 agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg tagttaaaac
    18421 gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta
    18481 aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat caacgcatcg
    18541 agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt ctcatttaaa
    18601 agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac cgacgccagg
    18661 catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg
    18721 tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag gtcgcgctgc
    18781 cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc ccgtggaaga
    18841 agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct
    18901 gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc
    18961 gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca ttccggatat
    19021 tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt
    19081 ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca tcaccacttc
    19141 gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta cgaaggcggc
    19201 gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta ttcgggtgaa
    19261 tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac aaacgcagga
    19321 taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac cggcggaact
    19381 ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca
    19441 cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg
    19501 gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg gcctacaaac
    19561 gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg
    19621 caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac
    19681 ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg
    19741 ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca
    19801 ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca
    19861 gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct
    19921 tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca
    19981 cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt
    20041 taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat
    20101 gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg
    20161 caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa
    20221 cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac
    20281 ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca
    20341 tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca
    20401 gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga
    20461 agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat
    20521 tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat
    20581 cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaaggcgac
    20641 gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg actacggagg
    20701 ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc
    20761 agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag gacgcaagtc
    20821 acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt ctcattacta
    20881 tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc
    20941 agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat
    21001 attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat ggcggacaaa
    21061 aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct cggcgcggta
    21121 aatagcgtga ttcagcgcgc ttcttcgctg gtctttgaca gtgtggaagc caaaaagcac
    21181 gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat
    21241 ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt gctatttccc
    21301 tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg
    21361 ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa
    21421 ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg
    21481 cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat ggaagtccac
    21541 gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat cattatgatc
    21601 gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct
    21661 attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg
    21721 tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg ccagatggtc
    21781 gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt
    21841 caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc gcaagtagcg
    21901 cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt
    21961 acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa tgaagagctg
    22021 gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa
    22081 tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc
    22141 gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt
    22201 gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg
    22261 cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg
    22321 aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc
    22381 tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc
    22441 tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct
    22501 gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc
    22561 ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg
    22621 ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg
    22681 gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg
    22741 tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat
    22801 ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt
    22861 gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa
    22921 acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc
    22981 tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg cctgacgatt
    23041 ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc
    23101 ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgca
    23161 atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga
    23221 tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg
    23281 tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct gccgccagtt
    23341 gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg cggctaatca
    23401 gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca
    23461 gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc
    23521 gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg
    23581 agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca
    23641 acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc ggcacctctg
    23701 acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg
    23761 catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta
    23821 tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac
    23881 gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta ttaactttat
    23941 ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc
    24001 ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag
    24061 acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag
    24121 gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta
    24181 atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac
    24241 aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaatcg
    24301 gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta
    24361 ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga
    24421 aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg
    24481 ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta
    24541 aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag
    24601 aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt
    24661 ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc
    24721 cgcgtcaggt ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta
    24781 ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa
    24841 tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca
    24901 tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg caggactggg
    24961 caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac
    25021 tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc
    25081 tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg
    25141 ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg
    25201 actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca
    25261 tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag
    25321 ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa
    25381 gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc
    25441 taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt
    25501 ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta
    25561 tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa
    25621 aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca
    25681 caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga
    25741 cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg
    25801 catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat
    25861 ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga
    25921 acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac acaaaatcca
    25981 gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat
    26041 tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc gctggcatct
    26101 ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt
    26161 tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa aactcgatca
    26221 gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa attctcccgg
    26281 tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc
    26341 cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg
    26401 aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac
    26461 agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc
    26521 ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg
    26581 ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac
    26641 aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg
    26701 atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt gcgataaccc
    26761 aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag
    26821 aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgacgata
    26881 ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg
    26941 ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac
    27001 atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg
    27061 ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa
    27121 tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac
    27181 gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt
    27241 ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg cgcgaaggca
    27301 agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg
    27361 caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat
    27421 aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    27481 ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca
    27541 aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac gcccacaaat
    27601 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc aggactattt
    27661 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    27721 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    27781 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    27841 gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag
    27901 cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    27961 gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt gccggatcgt
    28021 ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgt
    28081 cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg
    28141 agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt
    28201 tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga
    28261 aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    28321 taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    28381 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat
    28441 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg atttttttaa
    28501 tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca
    28561 tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg
    28621 gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    28681 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    28741 aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa
    28801 agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat
    28861 tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt
    28921 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca
    28981 tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    29041 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
    29101 acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt ttcggtgcca
    29161 ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt gggtcgattt
    29221 ttccaggggt atcaagacgg gtggaatcca cgccgctcca gccaggcagc gcctctttca
    29281 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
    29341 ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    29401 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt gcggtacggc
    29461 gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc tctttacaac
    29521 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    29581 aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc gaatccatgt
    29641 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    29701 tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc agcatacgac
    29761 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    29821 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    29881 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    29941 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    30001 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    30061 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    30121 ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata
    30181 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    30241 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    30301 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    30361 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    30421 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca tcggaagact
    30481 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    30541 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcgccaca
    30601 ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    30661 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    30721 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    30781 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt
    30841 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    30901 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat cataatggtt
    30961 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    31021 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    31081 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    31141 gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    31201 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    31261 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    31321 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg
    31381 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    31441 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    31501 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    31561 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    31621 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    31681 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    31741 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg
    31801 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    31861 aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg
    31921 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    31981 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    32041 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    32101 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    32161 agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    32221 cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga
    32281 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    32341 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    32401 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    32461 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca ggccctcccc
    32521 ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga tctcaacacg
    32581 atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac cagtgacttt
    32641 ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg tcagcgtgct
    32701 ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg cttctgccga
    32761 tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg
    32821 gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag catcttcaat
    32881 caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct tacctgcacg
    32941 gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat cggaagccat
    33001 cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc caggcggcaa
    33061 cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg taatcgggtc
    33121 gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc tcttaccttt
    33181 caccgccgct ttgaagctat cacccgcttt ataattcagg cccggcgcgt cgatatcatg
    33241 gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag gttcagacgg
    33301 cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg
    33361 atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt tattcatttt
    33421 acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct cactctgctc
    33481 accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt cgaggatcgc
    33541 ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg gttcatcttc
    33601 gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca aaccttccag
    33661 gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc gcacggtatc
    33721 gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg ccggacgacc
    33781 atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg tggtagcgaa
    33841 gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa tcaccagacg
    33901 ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca gctttttgtt
    33961 gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat gcggtaatgc
    34021 gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgca ctgaaccacg
    34081 tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg cttcagtcgg
    34141 ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg gctggtcgat
    34201 taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat cggtcgccat
    34261 gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag caggcagcat
    34321 tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc cctgccccag
    34381 ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac gcattgccgc
    34441 gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca gcggataacg
    34501 gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat cgccgtgcgg
    34561 atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt tggcgctggc
    34621 attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc
    34681 accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta agtaggcgtt
    34741 ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt ctgattcctc
    34801 aactgattcg ccagccttca aaggggcaat attgccggag atactacatt atctgccgcg
    34861 tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg gcgataaagg
    34921 catcaatgtg ccttgatata caactctttg ctgtagactt gatcggcata gagggcagca
    34981 caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg attcgagaag
    35041 ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgactg taagtatatg
    35101 gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca taagtgcggc
    35161 ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt tcgcgtatac
    35221 atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg gcattgttcg
    35281 ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca tcacgctaac
    35341 ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag attggtgtag
    35401 tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca gtggcgaata
    35461 atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc cacggcataa
    35521 cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat cggttcgcta
    35581 acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg gtcgtcccag
    35641 gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt acgccacatg
    35701 ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg caacatcgcc
    35761 acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt cattttgcct
    35821 ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg gatttacttt
    35881 gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt caccctgaat
    35941 ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt cgatatcaac
    36001 attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc gttcaacgat
    36061 attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt
    36121 tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg atgggccaga
    36181 aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta ctgcgattac
    36241 tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag catttaacaa
    36301 gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta cagagcgcgt
    36361 tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt
    36421 actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta gtaaaatggg
    36481 ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc
    36541 ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg atattttgcg
    36601 cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc gtgatgcgtt
    36661 agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta aaccttttgc
    36721 gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg gccaggccag
    36781 caacgagctg cgccacggta acgtcatgct cgaccccggc aaacgtatcg ccacgctggc
    36841 tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac tgatgcgtaa
    36901 cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct gggacgaaga
    36961 ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga
    37021 ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa atttacccaa
    37081 cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc ggtgacctgg
    37141 ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga attgttcgac
    37201 acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga aatcaacgcg
    37261 gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg
    37321 ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg cgataacgga
    37381 gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa
    37441 gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg catcgttgtt
    37501 ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg gcaattgatc
    37561 ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact gggtcgtgaa
    37621 ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa
    37681 ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc gctaaatcaa
    37741 ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc cgatgcagct
    37801 cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct
    37861 gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg gatcgatcgc
    37921 gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact ggataacctt
    37981 caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat ggatatttac
    38041 cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca cagcatcaaa
    38101 cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga taacgccgtg
    38161 cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa tttcatcgtg
    38221 agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga acgcttctat
    38281 cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt ccagcgaatc
    38341 gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg atttgaggcg
    38401 aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg cacattttgc
    38461 taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac cttgaggtta
    38521 aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact ccaatggtca
    38581 actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg ggcatgatgt
    38641 tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa actttctctg
    38701 ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt ggacagtgaa
    38761 aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca gcgatggccg
    38821 tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg gcaaaaaata
    38881 tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag atcaattctt
    38941 ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc aattccttgg
    39001 catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg atgttccccg
    39061 ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag agctcaggct
    39121 ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc ctggtcaaca
    39181 tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac tgaaagaaga
    39241 aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt tccagtctat
    39301 ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc ttgaagcgca
    39361 tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg tgctggagat
    39421 gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg ccatcctgac
    39481 cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct cttgcaacca
    39541 gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg
    39601 ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt
    39661 ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct ggacgttcgg
    39721 cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc
    39781 gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga cgtaaacgtg
    39841 accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca gcgtggcaat
    39901 gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac gaagctggct
    39961 cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca cttcctgcga
    40021 ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt tcagcggcat
    40081 gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc
    40141 cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca gtttgccagg
    40201 cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac gcatacgacg
    40261 ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt tctggttcag
    40321 ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt gacgcgaaga
    40381 gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg acagaacata
    40441 ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg
    40501 atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat taacatgggt
    40561 accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc
    40621 cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat taccgataaa
    40681 cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc
    40741 gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg taatctcaac
    40801 gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaatcg
    40861 cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt
    40921 accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa aggcgatgtt
    40981 ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg cgttaaccac
    41041 cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc
    41101 gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt ccgggtgaat
    41161 atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag catgtaagat
    41221 tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat caataacttc
    41281 ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg
    41341 ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt
    41401 tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga
    41461 aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg
    41521 aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca
    41581 gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat
    41641 ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga ccgagatagt
    41701 ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac
    41761 attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat
    41821 cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa ggatacggcg
    41881 gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag ttttttaaca
    41941 agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca
    42001 tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc gatgcacctc
    42061 ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatagtatc
    42121 cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcag
    42181 gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt
    42241 gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca gactgtgttg
    42301 atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg
    42361 ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc
    42421 gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa
    42481 cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa
    42541 atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa
    42601 atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc
    42661 gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt
    42721 tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat
    42781 cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta
    42841 ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa
    42901 ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt
    42961 aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac
    43021 catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg
    43081 cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg
    43141 atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa
    43201 caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg
    43261 tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca
    43321 ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc
    43381 ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccatgaat
    43441 accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc
    43501 ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca gtcccgcctc
    43561 ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta tcatcccggc
    43621 aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc gaatctgctc
    43681 aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg cgtgaccgcg
    43741 ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa
    43801 tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac
    43861 atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc
    43921 actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt
    43981 gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc
    44041 ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa
    44101 ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc tgggttcagt
    44161 tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg
    44221 gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc
    44281 agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc
    44341 gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt
    44401 tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg gattcaggac
    44461 gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc ttatttcaac
    44521 gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc gatctaccgt
    44581 caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac cgacgtgcag
    44641 aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt
    44701 gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga actggcggcg
    44761 ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct gctgaaagaa
    44821 gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct ggcgcgcgag
    44881 caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc ttctaccggg
    44941 atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac
    45001 gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat ttatcagggc
    45061 ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa
    45121 ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa caacattaat
    45181 gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta
    45241 gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt gttggatgca
    45301 accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa ctacctgatt
    45361 aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct gctggcactg
    45421 aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc gcaaacgccg
    45481 gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt cgttcagcaa
    45541 acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg acgacgacga
    45601 cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt
    45661 cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac
    45721 tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa
    45781 aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg
    45841 gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca
    45901 gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa
    45961 gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa
    46021 ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca ggcgcaggcc
    46081 cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg
    46141 atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccagccagc
    46201 ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc
    46261 cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac tactaccgtt
    46321 acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc
    46381 ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc
    46441 gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg
    46501 agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt gaaaagctgg
    46561 aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca
    46621 gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt gtgcgtcagt
    46681 catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg gatggcactg
    46741 gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac gaggcggcgt
    46801 tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg
    46861 accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg cgtgaacagt
    46921 atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca
    46981 ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacattg
    47041 acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtaatttcca
    47101 acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc accaagctgg
    47161 aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc
    47221 ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg
    47281 cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag
    47341 gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg
    47401 gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata
    47461 tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc
    47521 gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt gagtaatcaa
    47581 tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg acagcatact
    47641 caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
    47701 acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata acgcgccacc
    47761 ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc acgtcagatt
    47821 cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat caccgtgcca
    47881 cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta ttccttcatc
    47941 tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
    48001 gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca gcacgcccca
    48061 ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg acgctaacag
    48121 ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg tatcgtacag
    48181 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg cggtcactcc
    48241 tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc gtggcaatgt
    48301 catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
    48361 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
    48421 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
    48481 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt ataaggagtt
    48541 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
    48601 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
    48661 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cactagcagc
    48721 tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc ttggctattt
    48781 tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa aatcggtgca
    48841 gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca tcagtctgca
    48901 taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg agctgggatt
    48961 tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag tggcaggccc
    49021 ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga tttctggact
    49081 agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga tttccccggt
    49141 ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga
    49201 attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg
    49261 tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa
    49321 acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt
    49381 ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca
    49441 gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca
    49501 ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt ttacccttaa
    49561 tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc ggatatattc
    49621 atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa aagaatgcct
    49681 gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc atagcagggc
    49741 aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt aattaagcag
    49801 cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt cttcaatagt
    49861 cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac gcgccatcgt
    49921 gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg ccagcgtcat
    49981 caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg cctgcgcgcg
    50041 aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat cggcaatcgc
    50101 tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac cttcagccgc
    50161 ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta ccatcattgg
    50221 cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac cgctaccgtg
    50281 gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga tcatatcgcc
    50341 ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac gcagcgcagc
    50401 cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg tctgattcat
    50461 ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta ggagtggcgg
    50521 catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact ctctcccatc
    50581 cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga gttcgcaccc
    50641 aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt tcgccccgcc
    50701 ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc atagcttcac
    50761 ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca cctatcagta
    50821 cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc aggttcacga
    50881 atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa tccgccaaac
    50941 cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg acgtccaaac
    51001 gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa tgagcgaact
    51061 ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc ccccaacgct
    51121 gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt tacagtgctt
    51181 tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa tgagtgcaat
    51241 tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta gataaactaa
    51301 t
//
LOCUS       DAZRVY010000017        51278 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZRVY010000017 DAZRVY010000000
VERSION     DAZRVY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 51278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.017"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            228..1130
                     /locus_tag="ACHXOX_001019"
     CDS             228..1130
                     /locus_tag="ACHXOX_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7004561.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1277..2497
                     /locus_tag="ACHXOX_001020"
     CDS             1277..2497
                     /locus_tag="ACHXOX_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR7004562.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2470..3099
                     /locus_tag="ACHXOX_001021"
     CDS             2470..3099
                     /locus_tag="ACHXOX_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR7004563.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3100..4260)
                     /gene="ybdL"
                     /locus_tag="ACHXOX_001022"
     CDS             complement(3100..4260)
                     /gene="ybdL"
                     /locus_tag="ACHXOX_001022"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR7004564.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4369..5457
                     /gene="hcxA"
                     /locus_tag="ACHXOX_001023"
     CDS             4369..5457
                     /gene="hcxA"
                     /locus_tag="ACHXOX_001023"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR7004565.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5467..5664)
                     /locus_tag="ACHXOX_001024"
     CDS             complement(5467..5664)
                     /locus_tag="ACHXOX_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR7004566.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5846..7951)
                     /gene="cstA"
                     /locus_tag="ACHXOX_001025"
     CDS             complement(5846..7951)
                     /gene="cstA"
                     /locus_tag="ACHXOX_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR7004567.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8132..8545)
                     /gene="entH"
                     /locus_tag="ACHXOX_001026"
     CDS             complement(8132..8545)
                     /gene="entH"
                     /locus_tag="ACHXOX_001026"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR7004568.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8548..9294)
                     /gene="entA"
                     /locus_tag="ACHXOX_001027"
     CDS             complement(8548..9294)
                     /gene="entA"
                     /locus_tag="ACHXOX_001027"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR7004569.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9294..10151)
                     /gene="entB"
                     /locus_tag="ACHXOX_001028"
     CDS             complement(9294..10151)
                     /gene="entB"
                     /locus_tag="ACHXOX_001028"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR7004570.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10165..11775)
                     /gene="entE"
                     /locus_tag="ACHXOX_001029"
     CDS             complement(10165..11775)
                     /gene="entE"
                     /locus_tag="ACHXOX_001029"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR7004571.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11785..12960)
                     /gene="entC"
                     /locus_tag="ACHXOX_001030"
     CDS             complement(11785..12960)
                     /gene="entC"
                     /locus_tag="ACHXOX_001030"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR7004572.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13335..14300
                     /gene="fepB"
                     /locus_tag="ACHXOX_001031"
     CDS             13335..14300
                     /gene="fepB"
                     /locus_tag="ACHXOX_001031"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR7004573.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14381..15631)
                     /gene="entS"
                     /locus_tag="ACHXOX_001032"
     CDS             complement(14381..15631)
                     /gene="entS"
                     /locus_tag="ACHXOX_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR7004574.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15742..16746
                     /gene="fepD"
                     /locus_tag="ACHXOX_001033"
     CDS             15742..16746
                     /gene="fepD"
                     /locus_tag="ACHXOX_001033"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR7004575.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16743..17735
                     /gene="fepG"
                     /locus_tag="ACHXOX_001034"
     CDS             16743..17735
                     /gene="fepG"
                     /locus_tag="ACHXOX_001034"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR7004576.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17732..18547
                     /gene="fepC"
                     /locus_tag="ACHXOX_001035"
     CDS             17732..18547
                     /gene="fepC"
                     /locus_tag="ACHXOX_001035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR7004577.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGHTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18544..19677)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXOX_001036"
     CDS             complement(18544..19677)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXOX_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR7004578.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19893..23774)
                     /gene="entF"
                     /locus_tag="ACHXOX_001037"
     CDS             complement(19893..23774)
                     /gene="entF"
                     /locus_tag="ACHXOX_001037"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR7004579.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23771..23989)
                     /gene="ybdZ"
                     /locus_tag="ACHXOX_001038"
     CDS             complement(23771..23989)
                     /gene="ybdZ"
                     /locus_tag="ACHXOX_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR7004580.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23992..25194)
                     /gene="fes"
                     /locus_tag="ACHXOX_001039"
     CDS             complement(23992..25194)
                     /gene="fes"
                     /locus_tag="ACHXOX_001039"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR7004581.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25437..27677
                     /gene="fepA"
                     /locus_tag="ACHXOX_001040"
     CDS             25437..27677
                     /gene="fepA"
                     /locus_tag="ACHXOX_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR7004582.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27852..28472
                     /gene="entD"
                     /locus_tag="ACHXOX_001041"
     CDS             27852..28472
                     /gene="entD"
                     /locus_tag="ACHXOX_001041"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR7004583.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28594..28746)
                     /gene="hokE"
                     /locus_tag="ACHXOX_001042"
     CDS             complement(28594..28746)
                     /gene="hokE"
                     /locus_tag="ACHXOX_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR7004584.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29097..29249)
                     /gene="hokE"
                     /locus_tag="ACHXOX_001043"
     CDS             complement(29097..29249)
                     /gene="hokE"
                     /locus_tag="ACHXOX_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR7004585.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29218..29486
                     /locus_tag="ACHXOX_001044"
                     /pseudo
     CDS             29218..29486
                     /locus_tag="ACHXOX_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29689..30807
                     /locus_tag="ACHXOX_001045"
     CDS             29689..30807
                     /locus_tag="ACHXOX_001045"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR7004586.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30873..31121
                     /locus_tag="ACHXOX_001046"
                     /pseudo
     CDS             30873..31121
                     /locus_tag="ACHXOX_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31186..31554
                     /locus_tag="ACHXOX_001047"
     CDS             31186..31554
                     /locus_tag="ACHXOX_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR7004587.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31648..32301
                     /gene="nfsB"
                     /locus_tag="ACHXOX_001048"
     CDS             31648..32301
                     /gene="nfsB"
                     /locus_tag="ACHXOX_001048"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR7004588.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32409..33656
                     /gene="ybdG"
                     /locus_tag="ACHXOX_001049"
     CDS             32409..33656
                     /gene="ybdG"
                     /locus_tag="ACHXOX_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR7004589.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33724..35100)
                     /gene="pheP"
                     /locus_tag="ACHXOX_001050"
     CDS             complement(33724..35100)
                     /gene="pheP"
                     /locus_tag="ACHXOX_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR7004590.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35202..38345)
                     /gene="cusA"
                     /locus_tag="ACHXOX_001051"
     CDS             complement(35202..38345)
                     /gene="cusA"
                     /locus_tag="ACHXOX_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR7004591.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38357..39580)
                     /gene="cusB"
                     /locus_tag="ACHXOX_001052"
     CDS             complement(38357..39580)
                     /gene="cusB"
                     /locus_tag="ACHXOX_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR7004592.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39596..39928)
                     /gene="cusF"
                     /locus_tag="ACHXOX_001053"
     CDS             complement(39596..39928)
                     /gene="cusF"
                     /locus_tag="ACHXOX_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR7004593.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39952..41334)
                     /gene="cusC"
                     /locus_tag="ACHXOX_001054"
     CDS             complement(39952..41334)
                     /gene="cusC"
                     /locus_tag="ACHXOX_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR7004594.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQSS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41491..42174
                     /gene="cusR"
                     /locus_tag="ACHXOX_001055"
     CDS             41491..42174
                     /gene="cusR"
                     /locus_tag="ACHXOX_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR7004595.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42164..43612
                     /gene="cusS"
                     /locus_tag="ACHXOX_001056"
     CDS             42164..43612
                     /gene="cusS"
                     /locus_tag="ACHXOX_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR7004596.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            44350..46252
                     /locus_tag="ACHXOX_001057"
                     /pseudo
     CDS             44350..46252
                     /locus_tag="ACHXOX_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            46280..46741
                     /locus_tag="ACHXOX_001058"
     CDS             46280..46741
                     /locus_tag="ACHXOX_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR7004597.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            46761..51278
                     /locus_tag="ACHXOX_001059"
                     /pseudo
     CDS             46761..51278
                     /locus_tag="ACHXOX_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT        12423 a        14008 c        13295 g        11552 t
ORIGIN      
        1 attatgggaa ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa
       61 ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt ggctgtgcta ttttagaaaa
      121 acttaatcct gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt
      181 gattaagtga atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct
      241 acgacttgaa aaaattcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt
      301 taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt
      361 tacaacgctt acgtgcgcaa tttaatgacc ctgtattcat tcgctcagga aaaggtatcg
      421 cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg
      481 aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc
      541 ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct
      601 ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg
      661 ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt
      721 gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta
      781 ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat
      841 cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt
      901 catttcgtgg ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg
      961 gaatagtccc ctatgaactt tataactccc accgtgactt tcttaattta aaagagataa
     1021 aactagaaca tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa
     1081 ataatttggt gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta
     1141 cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa taaaacactc
     1201 tgctttacag tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt
     1261 cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata ttctggaagc
     1321 tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg tctcattttc
     1381 tggcggcaaa aactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg
     1441 caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca ccattaacta
     1501 tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact gggttgcgct
     1561 cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt gctgggaacc
     1621 tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg actttttctg
     1681 cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg aatggttttc
     1741 acaaaaacgt ccggtggcga tgatgatcgg catccgtgcg gatgagtcct acaaccgttt
     1801 tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccat ggactacggc
     1861 cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag tggctgatat
     1921 ctggacctgg tatgcaaatc atcaaaatct ctgcaaccca ctgtataacc taatgtatca
     1981 ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg agcaacgaca
     2041 aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt gcgcacgagt
     2101 cagcggggta aaaagtggcg gcatttacgc cggacatgat aatcatttct atgggcatcg
     2161 gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc tgctcaatag
     2221 catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt tgcactggta
     2281 tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg cgaaagatat
     2341 cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt gtcgggcatt
     2401 atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga taaaaggaaa
     2461 acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac gcaatttctc
     2521 gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat ggcgatccat
     2581 caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa aaacagccaa
     2641 cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa actgctgcaa
     2701 aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacacatac ggataaaaat
     2761 gccatggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc atcatcatta
     2821 aaattacgct taaaaggtta tttgccggtg atctgtctgg aaggtacgcg taatcaacgc
     2881 atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac cgccatgtcg
     2941 gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg caaagagctg
     3001 ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca agagctgttt
     3061 gccgaccgtc aatattcccg cgcctggacg gttaaataac taaagctggc gcaggcgttc
     3121 agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca gtttatgtgg
     3181 gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct cctgcgtcag
     3241 ccactggcaa aactcaacat catccagggt agaaaccgcg ctgtaatcca ccagcaaaaa
     3301 gtatgtacct tcacacggta aaatctccag ccggctttca tttaaggcat ttaccagaat
     3361 atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag
     3421 catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat actgatgtac
     3481 cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa taacccactt tccagccggt
     3541 catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca gctgcgaatg
     3601 cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga cttcatcgct
     3661 aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat cagcctgctg
     3721 ccagacagtt gcactggagt tatgcggagt gttgaggatc accagtctgg tgcgctcgct
     3781 taataatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca gtgccatacg
     3841 cttcactatt cccccagaaa gcgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa
     3901 acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata acgcttccgt
     3961 cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat acaaacgttc
     4021 cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg cgtattggtt
     4081 tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac catcaaaatc
     4141 aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc tcatctgggt
     4201 gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt tatttgtcat
     4261 ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc tagtatttgg
     4321 caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat gcctcacaat
     4381 cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag tttcaatcac
     4441 ctgaacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta cggcaaacgc
     4501 gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg ggcaaagcat
     4561 attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc tgagtccggt
     4621 gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac cgcgaaagcc
     4681 ctcgcccgcc gtctcggtct gccatttgtt gccgttccga cgatcgccgc tacctgcgcc
     4741 gcctggacac cgctctccgt ctggtataac gatgccggac aggcgctgca ttatgagatt
     4801 ttcgacgacg ccaattttat ggtgctggtg gaaccagaga ttatcctcaa cgcgccgcaa
     4861 caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg
     4921 gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg ggatcaataa tgcgcaggcc
     4981 attcgcgacg tattgttaaa cagtagcgaa caggcgctga gcgatcagca aaatcaacag
     5041 ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg gatggtcggt
     5101 ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa cggtctgact
     5161 gtcctgccgc aaaccgagaa atttctccac ggcaccaaag tcgcttatgg cattctggta
     5221 caaagcgcct tgctaggtca ggatgatgtg ctggcgcaat taaccggggc gtatcagcgt
     5281 tttcatctgc cgaccacgct ggcggagctg gaaatggata tcaataatca tgcggagatc
     5341 gacaaagtga ttacccacac cctgcgtccg gtggaataca ttcattacct gccagtcacg
     5401 ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt
     5461 gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg
     5521 gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca tatgttcgac
     5581 atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta aatattttcc
     5641 ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg aaagagtgcc
     5701 ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc ggtcagtccc
     5761 ctcgccccat cggggagagg gttagggtga ggaggaaccc tcggcactat gccaacattc
     5821 gccaacatcc cccctcactc tgactttagt gtgcgccttt tgcctgcgcc acgatctcct
     5881 cgacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc ggatctttca
     5941 atgccgccag tgccgactta atcgagaaca gcgccagaac caccacgacc accataaaga
     6001 agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc tgtgactcag
     6061 tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttatta gcaatggcca
     6121 ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc agggtacaaa
     6181 tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt ttcatcttga
     6241 acaacaccac ggcacagagc atcagcgcca tccctgccag catctggttg gcaataccaa
     6301 acagcggcca cagagtgtta atgccgccca gcggatcgac cacgccctga tggaggaagt
     6361 agccccacgc cagtacgcac agcgctgttg ccagcaggtt agcaggcagt gaatcggtcc
     6421 gcttcaggcc aggagacacc acgcccagca gatcctgcaa cataaagcgc gcagcacgcg
     6481 tacccgcatc taccgccgtc agaataaaca gagcttcaaa cagaatggcg aagtgatacc
     6541 agaacgccac atccatcata ccacccagcg caccgtgcag gatgtaagcc atccccaccg
     6601 ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact tcgctggcaa
     6661 tctggttgag cgtatccgga gtaatcgcaa agccccagct actcaccacc tgcgcggcag
     6721 aagcgaccac atccgccgtc cctgccggag ccagcaccgc catcgggctg ttcatggcga
     6781 agtacacgcc cggatcgatg atacaggcgg aaaccagtgc cataatcgcc acgaaggatt
     6841 ccattaacat tccaccgtag ccgataaagc acgcctgccc ttcgttcgcc agcatcttcg
     6901 gcgtggtgcc ggaagagatc agcgcatgga agcctgacac cgcgccacag gcgatggtga
     6961 taaacaggaa cgggaacaga ttaccggtcc ataccgggcc agtgccatcg acaaatttgg
     7021 tcagcgcagg catggtcagc gtcggacgca taatcaaaat gcctaccgcc agaccaacga
     7081 tagtcccgat tttcaggaag gtagagaggt agtcacgcgg ggccagcagt aaccacaccg
     7141 gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc acgccggtaa
     7201 agtcaaagta cggtgcccag gtcgggcttt ctgccaccca gccgccagag ataatggcaa
     7261 aaataaggaa caccaggcca atcactgaca cttcgccaat acgccccgga cgcagatagc
     7321 gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta tacgttcccc
     7381 acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg ataatgacca
     7441 tgatcataaa gcaggctacc agcgcaatca ccccggcagt tggccccatc tcttctttga
     7501 ccagctcacc cagcgagcga ccgtcacggc gcgtagaaac aaacagcacc atgaaatcct
     7561 gcaccgcacc ggcgagaacc accccggcga gcagccagat catccccggc aggtagccca
     7621 tttgcgctgc cagcaccggc cccaccagcg gccctgctcc ggcaatggcc gcaaaatggt
     7681 gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta tggcgcaccg
     7741 ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac agcccataaa
     7801 aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc gcgttgatct
     7861 gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca gagagcactg
     7921 tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt gtccatagag
     7981 atgttacatt ttgtttctat aacatctttg tacgatttaa caacacggta accatgtaaa
     8041 tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg ttaaccgagt
     8101 tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta cccagccgac
     8161 aagtgcagca acgccgcccc tgttcatcga aaacgatgat ttcccagctc tgattttgcc
     8221 gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc gggcgatgat
     8281 gggtggcgtt aagttccgta cccaccacac actgcccgtc acgggtcatc ataaatccgg
     8341 ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc aggccaaacg
     8401 gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct cccagacggg
     8461 tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc agttcgtcga
     8521 gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc catcgaccac
     8581 aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa tcgtgttggc
     8641 gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact gctcgccaaa
     8701 gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt gcatatcggt
     8761 gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctaaccgcca gttccagccc
     8821 gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac tcataccaat
     8881 acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc gctgacggcg
     8941 aaactggttc atggtttgct gaaacaggtt aaacgcaccg ccgacgttaa ccgcaaaagt
     9001 ctgctgccag tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa ttcccgccgc
     9061 attgaccagc acgtccagtc gctccgtttc agctaacagt cgctgacata cctgcgcgac
     9121 ctgcgcagca tcggcaacat ccatcacttc ggtcgcaaag ggatattgct cctgagcgaa
     9181 cgcttgatca aaaccggtaa ctttcgctcc cgcttcaaca aacgccagcg ccgtggcgta
     9241 gccgatacct ttacctgcgc cggttaccca gacattttta ccgctgaaat ccattatttc
     9301 acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc cagcatgaca
     9361 aagtcgatat cgccatgcac tttgcgccag cgcgccgcca gcgccatcat gcgcaccgaa
     9421 tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc gtccagcaac
     9481 ggcaggataa cctcacgcag cgccgctttc ctggcgggga caggtgctgg cagtaatcct
     9541 tcagtcatca ccacccggcc agaccgcccg gccacatatt tcagcgacat caaatgctcg
     9601 tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc gcgcataaat
     9661 gcgtcggttg cggtggtcat acagccaatg tgggcatata ccccggtaat aatcagctgg
     9721 ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc gctgtagcgc
     9781 cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac cttttgctgt
     9841 tccggcgaac gggtcaggcc cggcccccac atatcattca acagcgcccg atcttcatcg
     9901 ctctgctctt tcggctgggc ggtgtaataa accgggatat tgtgctgttt gcagtagtcg
     9961 cgcagcgcag caatattcgc gatcacctgc tccatcatcg ggcagttctc gccccagaag
    10021 ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg ttcaaaggcc
    10081 cagtcaactt tattctgcgg aatatcgtga gactccggca gtgcgtaagc ctgtaatttt
    10141 ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc actgacgtaa
    10201 ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatccacac actccacgcg
    10261 atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca cctgcaccgc
    10321 gcgcagcggc tcttttacca ccagataagc acagcttttt tcgcccatca gctcatcttc
    10381 catgctcacc agtgcggcat agatcaccgc cggatggcgc agcagcaggt tttcgatctc
    10441 ttcggcagcg atcttctcgc cgccacggtt gatctgatct ttctcgcgcc cctgcacggt
    10501 gatgtaaccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac cgttggcatc
    10561 aaaggcactg gcattgtgct gcggactttt gtaataaccg cggaaggtgt acggcccgcg
    10621 cgtcatcagg cgtccaactt ccccttgcgg cagtggattt ccttcagcat cagcaaccca
    10681 tacttcatcg tccggacaca ttgggtaacc ctgggtatgg ataattttct ccgcgctatc
    10741 atcaagtcgg gtgtagttca ccagcccttc cgccatgcca aacacctgct gcaactggca
    10801 gccaatctca gcggaaatac gcgccgcaag ggtggcagaa agacgtgcgc cgccgacctg
    10861 taacagtttc agcgaggcaa gctgcgcccg gctttcgcct tcagccagcg cctgcaacca
    10921 caggctgact gccggcggca ccagcgcggt aacgttaacc tgatgttttg caatcaacgg
    10981 gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg caagaaagac
    11041 gcccagcgat cctggcgaac tcatagcgta gttatgagcc gccgggatcg cgcacaggta
    11101 gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt agtagtagtc
    11161 gttatgagtg cgcgggatca gcttcggtgt accggtggtg ccgccggaaa gctggaaata
    11221 ggccacttca tcagcaggtg atggcgtagc agtaaaaccg tctgccggat ggttaatcgc
    11281 atcctgcaag ttatgctcac cgctgtcgtt gagcagttgc accacgcgaa tggaggaatg
    11341 ttctgtgacg aaagtattga ggaaatcatc cccgctaaac agcgcatgtt ggcgatcggc
    11401 aatcagtaac gccggttcaa tctggctggc ataggcgttc agttcactac gctgatggct
    11461 gaacaacgcc agcaccggcg caacgcccag tttcagcagt gcgaaaaagg taatgtaaag
    11521 ttcagcgacg ttacccagtt gtaccagcgc ggtttcaccg ggtttaatgc cctgacggcg
    11581 taaactacac gcgaggttat ccgccgcctg attcagctcc cggtaactca actgtcgctc
    11641 gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt cggtcagcgg
    11701 caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg gccagcgggt
    11761 gaatggaatg ctcatcctcg ctccttaatg caatccaaaa acgttcaaca tggtagaaag
    11821 tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa tccccgctcc
    11881 ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca ccacccattc
    11941 gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc ggtcaaacgg
    12001 ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca gcgcgggggt
    12061 cggatgcagc agacaggcca gagtcagtgc gttttcttgc gaattcgctt taccttcaaa
    12121 gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg agggaacgtg
    12181 taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca gttcatgttc
    12241 atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga gcacttcatc
    12301 cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc cgtctttacg
    12361 tagcagcagt tccgggctgg cccccagcag gacgccacca tcagccagcg ggacatggaa
    12421 gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac tatcaatggc
    12481 ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact ttgtcgacct gcggcgtggc
    12541 ggtaagtgcg gcggcgcggg caaccatctg ttcaaacgtg gtttgttcag gaattgcctg
    12601 gcgttccacc acgttcagcg actggctgcg ggtaaaacag tgggctgagg tctgtttttc
    12661 ctgacgggag aacgactgcc aggattcggg aatatacagc gacgaaggct gacgtggatc
    12721 gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg catcggcaaa
    12781 cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca cagccggttc
    12841 atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca taaagaaaaa
    12901 gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg acgtatccat
    12961 atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat tttcggtcgc
    13021 taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct gcaactcggg
    13081 gttgcatctg gttgtggtta caatgaaaat gagaagcatt attgatgaat tcgcataagc
    13141 gcaatgtgat gtcctgcgcc gttttgcccc ctctcccttc cagggtgagg gctggggtga
    13201 gggttaatgt tcgcaccagt gctggctgtt cccctcaccc taaccctctc cccaaagggg
    13261 cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac gcccttaacc ttattaataa
    13321 caggaagctg acttgtgaga ctcgccccgc tctaccgcaa cgcccttcta ttaacaggac
    13381 ttttgctttc aggaatagcc gtagttcagg ccgccgactg gccgcgtcag attactgaca
    13441 gccggggcac tcataccctg gaaagccagc cgcagcgtat tgtttccacc agcgtcaccc
    13501 tgaccggatc actgctggcg attgatgctc cggtgatcgc cagcggcgcg accacgccga
    13561 ataaccgcgt cgcagatgac cagggctttt tacgccagtg gagcaaggtg gcgaaagaac
    13621 gcaaactgca acggctctat atcggcgaac cgagcgccga agccgttgcc gcgcaaatgc
    13681 cggatctgat tttaattagc gcaaccggcg gggattcggt gctggcgctg tatgatcagc
    13741 tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg cagtcgctgt
    13801 taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg attgcacagt
    13861 ttgataagca actggcagcg gcgaaagagc aaatcaaatt accgccgcag ccggtcactg
    13921 ccattgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa tcagcacaag
    13981 ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg ttaaacgcca
    14041 gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggggaaaat ctggctgctg
    14101 ggttaaacgg tgaatcgcta ttcctgttcg ccggtgatca gaaagacgcc gatgcgattt
    14161 atgccaatcc actgctcgcg cacctgcctg cagtacaaaa caagcaggtt tatacgctgg
    14221 gaaccgagac gttccgcctg gattactaca gcgccatgca agtgctggac agacttaatt
    14281 cactgttaaa aaaagattga taaaacatac aattagagca ctaataatcc cctcgcccct
    14341 ttggggagag ggttagggtg aggggaaaat gcacctttag ctaactgtca gacgctgtca
    14401 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    14461 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    14521 ccaaaccacc caacagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    14581 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    14641 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    14701 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    14761 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    14821 caccgagcgg gatcgccgca tagagaaaac caatctgtgc cgccgacatc tgccagttgt
    14881 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    14941 acgcaatccc tcctaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt
    15001 tcaacggatg ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    15061 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccgccggtcg
    15121 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    15181 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgcaggtgtt gccgccagta
    15241 gcgccgtaac accaagtgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    15301 gcaatgacgg ctccggcagc agcgcgttca ggcacagtcc aatgaagcca atgccacagg
    15361 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    15421 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg
    15481 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    15541 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    15601 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    15661 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    15721 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    15781 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    15841 tcactccccg cgtcagtagt gctggaggcc ttctcaggca cctgccagag cgccgactgc
    15901 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttacttgc aggcggtgcg
    15961 cttgggcttg ctggggcgtt aatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    16021 ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    16081 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    16141 attgttgcct ttatcggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    16201 gcgggcgtgg cgctggcggc ggtgctggaa gggctgacca gcggcatcgc cctgcttaat
    16261 cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    16321 cataccttaa aagtggtgct gatcccggtt ctgatcgccg gagcaactgc gctattactg
    16381 agtcgcgcgc tgaacagttt gagcctcggc agtgacaccg cgacggcgct gggcagtcgc
    16441 gtggcgcgca cacagttgat tggtctgctg gcgattaccg tgctttgtgg tagtgcgacg
    16501 gcggtagttg gcccgattgc ctttatcggc ctgatgatgc cgcacatggc gcgctggctg
    16561 gtaggtgccg atcatcgctg gtcgctgccc gtcacgctac ttgctacccc tgccctgctg
    16621 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggcg aactgcgcgt ttctgtggtc
    16681 agtgcgttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt
    16741 gcatgattta cgtctctcgc cgattactca tcacctgttt gctgctgatt tccgcctgtg
    16801 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaatc tcgcaggtat
    16861 tcgccgcgtt gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    16921 taccacgcgt gctgatggcg ctgttgattg gcgcggcgct gggcgtcagc ggcgcgattt
    16981 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    17041 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    17101 tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta
    17161 acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg
    17221 cctttaatac ttggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    17281 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca
    17341 tcatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg
    17401 gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc
    17461 tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg
    17521 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    17581 aggcggcgct gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    17641 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    17701 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    17761 cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    17821 tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact
    17881 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    17941 cattcaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc
    18001 taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca
    18061 accgctgttt acccgctggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc
    18121 cacgagaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    18181 gcgagcgtgg atcgcgatgg tgctagccca ggaaacggca attatgctgc tcgacgaacc
    18241 cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa
    18301 ccgcgagaaa ggccataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    18361 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    18421 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    18481 tccggtagcc ggaacgccgc ttgtggtgcc gctcggacga acggcacctt caaccgcaaa
    18541 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    18601 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    18661 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    18721 cgtaaaattc acatcgttga catttgcttt tgttaattgc tcgacaagat actgccgatt
    18781 acgtaattca ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    18841 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    18901 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    18961 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    19021 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    19081 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt
    19141 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    19201 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    19261 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    19321 ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    19381 gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    19441 ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt gcggcaggat
    19501 gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga ccgttttttt
    19561 ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattattac tgggcgacgc
    19621 cagaggatac tcagaaaaat gagcgtcact tccctgttta atattcaatg atgacatggg
    19681 gtataacctg aaaatgagtc caatagggga aaatctccac ggcgctcact ttaccagttg
    19741 gtgagaatta tccgaagctg aagtttgtaa attccttcca ctgaatgcaa tattcactct
    19801 atttaagaga aatattcatc aaacctataa atcaaacaaa taaaaaaccc ataattattt
    19861 tctgtaatta tgggtttata gataatatta atttacctgt ttagcgttgc gcgaataatc
    19921 ggcccaattt ttacaaacgc ccctggagag ataatatcca catgcgcaca atcctgacga
    19981 tagatatcca gctccgcaat ccacggcgac caggcgcgtt caggactcat accttcctga
    20041 agcgtgcgtt cagcaacaaa caacgtcgct ttaccgtcaa acggtacgct atgagcagtc
    20101 gtcagcaggc gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa ctccgttgaa
    20161 gtacttccct gctgtgccgc caggaaggcc tcgcgctcgc ggttaatctc cgccagcact
    20221 tccgggtcca gaccattagc ttctttttcc tgccagtttt gcgtttctgg cggccaggta
    20281 tccagcaagc caagaaatgc cacctgttcg ccacgggcac gcagtcgcgc cgcaataccc
    20341 tgcgccagcg taccgccaag ggaatacccc agcaggtaat aagggccgtg cggttgttgc
    20401 tcaagtaacg ttgccagatg cgcttcgcag acttcatcca ggtttgtcgc tgtctgcatg
    20461 ggtccatgag ggcgcggcga ctgaatgcca ataatcgacc attgtggatc gagataacgc
    20521 gagagtacgc taaactgcca ggcaaaaccg gacgccggat ggaaacaaaa cagcgtcggg
    20581 ccattacctt cacgcaacgg cagaatggtt tcgaatccca tgcgccggga gctgtcctct
    20641 tcaccatcaa taatcgttgc cagtttggcg acggttgacg cgaccatcac ctgccccggc
    20701 gtcacctgac gagcaaactg tcgacttaac tgcgctgcca gtttcattgc cagtagcgaa
    20761 tgaccgccca gcgcaaagaa atcagcatcg gcatcctgca cgtcacaacc cagcaacgac
    20821 gcgaatgccg cggcgataat cgtttcactg cccgctttcg gtgcacgtcc cgacgcttgt
    20881 gtcttcagtt caggcaacgg taaggctttg cgatccagct tgccgttggc gctaagaggt
    20941 aactgtggaa gttgcagcag aaccaccggt accatatgtg gtggcaatgt ttcacgaagc
    21001 tgcgcctgca atgcgctggt atccaacggc aggcccgatt gcgacaccag atagcccacc
    21061 aactgacgcg catcaccacc agtggctgcc gcctggttaa tcacacaggc gtgggtaacg
    21121 gcttgttcga catccggcag cgcctgcatc acgcgatcga tttcgcccag ttcgatacgc
    21181 tgcccgcgaa tttttagctg atcgtcactg cgcccgaggt actccaccgc gccgttatcc
    21241 agccagcggg caacgtctcc ggtacggtac atccgttcgc caggagcaaa aggatcggca
    21301 ataaagcggc tggcggtcag atcggggcgt ccgagatagc cctgcgccag ttgaatgccg
    21361 gtaagataga gatcacccgc cacacccggc ggcaccggat gcatcatcgc atcaagaata
    21421 cgcagcccca tgttccacac cggataacca atcggcacac tgctgccgcg cacctgtgcc
    21481 agttcctcgc caaaagccgg ataccagctg acatctaccg ccgcttccgt cgggccatac
    21541 agattatgta acggcgcacc ggttaactgt tgccattcac ggcataaatc agccggtaag
    21601 gcttcaccac tacagaaaac ctgtttcaac gtcgcgcaac tctggcgagc ggtttgcgga
    21661 gtcagcgagg caacaaatgc cgccagcatc gacggcacaa agtgcgtggt cgttacgcca
    21721 tattcggcaa agaattgctg catagcgagc gggtcacggt gcgcttccgg ttcagccatc
    21781 accagttttg cccctgcgat aaacggccag aaaaactccc acaccgagac atcaaaactg
    21841 cacggcgttt tttgggcaac gacatcttcg cctgtaagcg ggtagtgatt ttgcatccat
    21901 aacaggcggt taacgatagc cgtctgcccg accattaccc ctttcggcct gccggtggag
    21961 ccagaggtaa agatgatata agccgtgtgg tgcggttgtg aaagttgcag cggcgcactg
    22021 ccctgggggg taagcggggc gttatagcaa aggcttgtta aattgggaac atcgctaaag
    22081 cgcggcagtt gatcgtcggt ggtaattaac agcgacggac gcgcatcttc cagcatcatt
    22141 ttcaggcgat cgtccggata gccggtatcc agcggtagcc aggccgcgcc agcttcaact
    22201 attgcatgga gtgccagggt caaaaagacc gagcgcggta gtgccaccgc cacgctgtcc
    22261 cctggtttaa cgccgcgctc acgcagcaga ttcgccagcg ccaccacctg ctcgcgcatt
    22321 tcccgatagc tgaacaggta acgcgcatct gccagcgccg gagcatccgg tgtttttgcc
    22381 gcttgttctg ccaccagcgc gctcagcgtg gtttctggaa tctcaacctg agtggcgttg
    22441 atctgcgcca gctgcgcata ctcacctggc agcataatat cgacatcgcc gcacaacagc
    22501 gccggatccg cggcgaactg ggcaatcagc attttcaggc gttcagcatg ctggattaac
    22561 gttggctcat cgtaacgctg tttattggcg aggatctcaa tactcaaatc accgtgttca
    22621 tccgggaaca gggccagttc aaggtcatta accggaccgg ttgccagggt atgggtttgc
    22681 gcctgaacac caggaatatc cagttggtaa tcaaatacct taatattgag taccggacca
    22741 aacagcggtt catcacctgc cgctcgcccg ctgtcacgga caatttgttc ggcatcgtaa
    22801 cgttgatggc gacgcatttt tttcagttga gccgccagtc gggttgccag ttccggcagc
    22861 gtttcttgcg ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg tccgctagcc
    22921 gtcagcgccg ccgagcccag tcgacgcata aagataaatc cggcggcata gtccatgcga
    22981 ttgcacaatc gccccagcca caaggctgcc agcgcaaggg ctaaatcggt acgctgcaca
    23041 cctgaaagtt gcgtagccag ctggcggaat tccccgtcgg taaattccag tttcaggcgc
    23101 agaatatctg ccgaagcgct gcgccccggt aaaggtgccg gagaaagtga cgcgggcggc
    23161 ggcagttgac gacgctgttc tgcccagaat gccgcatcac gctgccaggc ttcgctttcg
    23221 cggtattgct ggtactcttc cactacatca gcgaaaggcg taaacggcga agcaggcgtt
    23281 ggttcgccac gcagccatgt gcagtaaata ttggcgatct ggcgagtaat ggccgggaaa
    23341 ctgaagccat cgaccagcaa atggtgataa cgctgatacc agtaccagcg gttatccgca
    23401 acatgtatca gctgatgaaa gaccagtggt ttaccgctat cgacgcgcaa atcttgttgc
    23461 aaatccgtca gcattaatgc ctgcgcagta ccgtgcggat caatattggt tcgcaggtcg
    23521 ataatttctg gctgttcgaa tatcagcgca tcatcgaccc actgccagac ttcgccgtta
    23581 tcttccgtaa aacgcatccg caacgtatct gcttgcgcta gtccggcaac caccgcgcgg
    23641 gccagtaatg gcgcatcaac atctccagtt aactcaacgt aatgcgccac gctccaggcg
    23701 gagggtaaat ccgacagttt ttctgccatc cagatgccgg gctgtgcggc gaccaaaggt
    23761 aaatgctggc tcattgtgcc tcctgcaact gggtgaaatt ggccggtgtc agagtacgcc
    23821 agtggacttc cagccactgc tggcaggacg cctgtgactg cggctgacac acaatgtccc
    23881 agcctgccga taagaggcat tgttgcggcc acagactgaa ttgcccctgc gcattgcgca
    23941 aaatgtaaaa cgctccctgc ggatcatcga agggattact gaatgccata ctcaactcct
    24001 gtcatggaaa agtggttgcc agaggtcgat taacccctgc atcaagccac cgcgccaaca
    24061 aagcgcatca tgtccgccgt caacctgacg ccagaaaatg gattctttta tagggtgcaa
    24121 ttgcgcatac agcgcctgat tggcccgcat gatcatcggc tcgcgaatac ccgcttccag
    24181 cacaatgcgc agaccttcgg cgctaacttc accagctttt agcttttcaa gtaacacgcc
    24241 ctcttgctgc ccgtcccgat gcggccacca gtacgatcct gactggctta atacacagcc
    24301 aaagcgttca ggccagtgca gtccggcata cagcgcggac agcccaccaa aactctgccc
    24361 ggcgaccacg gtgcgatcgg cgcgatcgct aaaaggggca atagctttca ccaggggtaa
    24421 taactcttgc tgtactgcca gccagaaatc cgcattacac ggcagttcgt gggcgcggtg
    24481 cgtggtgtcg atagcgtcga tcaacacata cacggcggga ggaagttgct gacgatgggt
    24541 cagcgaagtc agcacaggcc agacgggcat actttgcgcc caaaattcgc catcgagcaa
    24601 aactgccagc ggacgttctt ctgctgttac atcgccggtg gtaaaaatcc atacacgacg
    24661 tgaatttttc aaccgttcac ttttccagat aatttctttg gctgctattt ctggcacttg
    24721 cggacaatcc catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag aaacagcgtg
    24781 ccctcgcccg cctttccagc tttgtggatt cagcggatcg gctattgcct ggggtaatag
    24841 ttttcgccag ccttcacgta attcgaggcg atcgggggat ggtgcagaaa aaatgtcatc
    24901 gcgttcggtg ggaataaagc agtagctgcc gcgccagttg gcattgagtt gtgtcgtcca
    24961 ctgccagaca tcagtacctg caattcgctg catcgactgg ggctggctgt tctgatgatg
    25021 atcggtcaca ccagtgatgt agatccatac gcgctttatc gtcgagtatt cttcagaacc
    25081 ttggggatca cgccaccaga aagtgacctc aaacatttcg tcattcagac gctgccattc
    25141 cgggccatgt ttcgactgcc accagctctc acttcctact tttaacgccg tcacaccata
    25201 accccatgtt tactgtgcaa tttttcattg attgcagaaa tatattgata atattattga
    25261 taaccatttg catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga catttattaa
    25321 ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga tgcgtcgtgt
    25381 tgaggacgac catgcccgac agttgcaatt cgtggcaaaa atgcaggaat aaaacaatga
    25441 acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg gtagcgcagg
    25501 cacaagagcc gaccgatact cctgtttcac atgacgatac cattgtcgtt accgccgccg
    25561 agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc cgcaaaaacc
    25621 ccgttgcccg cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac ctgaccggta
    25681 actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt atgggtccgg
    25741 aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg gtgcgtcagg
    25801 gctggcgtgg cgagcgcgat acccgtggtg atacctcctg ggtgccacct gaaatgattg
    25861 aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc gcggcgggcg
    25921 gcgtggttaa catcattacc aaaaaaggca gcggtgagtg gcacggctcc tgggacgctt
    25981 atttcaatgc accagaacat aaagaggaag gtgccactaa acgcaccaac ttcagcctga
    26041 ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac aaaacccagg
    26101 ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg tatgccacga
    26161 cgttaccagc cgggcgcgaa ggggtgatca acaaagatat taatggcgtg gtgcgctggg
    26221 atttcgcgcc attgcaatcg ctggaactgg aagcaggtta cagccgccag ggcaacctgt
    26281 atgcgggcga cacccagaac accaactccg attcctatac ccgctcgaaa tatggcgatg
    26341 aaaccaaccg tctgtatcgc cagaactact cgctgacctg gaacggcggc tgggataacg
    26401 gagtgaccac cagcaactgg gtgcagtatg aacacacccg taactcgcgt attccggaag
    26461 gtctggcagg cggtaccgaa gggaaattta acgaaaaagc gacacaggat ttcgtcgata
    26521 tcgatcttga tgacgtgatg ctgcacagcg aagttaacct gccgattgat ttcctcgtta
    26581 accaaacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac ttgagttcca
    26641 atacccaggc gctgaccggg gcgaacaccg gcggcgctat tgatggtgtg agtgccaccg
    26701 accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac aacatggagc
    26761 tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt attgtcggca
    26821 ataactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc acgctcaaaa
    26881 tgggcattgc ccgtgcttat aaagcaccga gcctgtacca gactaacccg aactacattc
    26941 tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatcag caaggtaacg
    27001 atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc aaacgcgacg
    27061 gctggctggc gggcgtcacc tggttccgta acgattatcg caataagatt gaagcgggct
    27121 atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat aacgtgccga
    27181 aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa atggtgatgt
    27241 ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc gaccgtttgt
    27301 cgatcatccc ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg gaagatttgt
    27361 cgatgcaaac gaccttcacc tggtacggta agcagcagcc gaaaaaatac aactacaaag
    27421 gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt gtgggtctga
    27481 gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac aacctgttcg
    27541 acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca ggggccaact
    27601 atatcgcggg tgccggggcg tatacctata acgagccggg acgtacgtgg tacatgagcg
    27661 taaacactca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc gttcatgccg
    27721 gatgcggcgt gaacgcctta tccggcctac aaaaatcttg ccaattcaat atattgcagg
    27781 accatgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca tcagtctcga
    27841 atatggtcga tatgaaaact acgcatacct ccctcccctt tgccggacat acgctgcatt
    27901 ttgttgagtt cgatccggcg aatttttgtg agcaggattt actctggctg ccgcactacg
    27961 cacaattgca acacgctgga cgtaaacgta aaacagagca tttagccgga cggatcgctg
    28021 ctgtttatgc cttacgggaa tatggctata aatgtgtgcc cgcaatcggc gagctacgcc
    28081 aacctgtctg gcctgcggag gtatacggca gtattagcca ctgtgggact acggcattag
    28141 ccgtggtatc tcgtcaaccg attggcattg atatagaaga aattttttct gtacaaaccg
    28201 caagagaatt gacagacaat attattacac cagcagaaca cgagcgactc gcggactgtg
    28261 gtttagcctt ttctctggcg ctgacactgg cattttccgc caaagagagc gcatttaagg
    28321 catcggagag acaaactgaa gcaggttttc tggattatca gataattagc tggaataaac
    28381 agcaggtcat cattcatcgt gagaatgaga tgtttgctgt gcactggcag ataaaagaaa
    28441 aaatagtcat aactctgtgc caacacgatt aattgacaac atctggtacg attcgcccgc
    28501 agccatcact gactgcgggc gaaagtgtaa agcaggtgcc ttaccactct gacctgacaa
    28561 ccggatatgc agggattgct ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    28621 agccttaaac tcaatattac gttcctttac ggtgaactca cacagcgagt ctccgaccag
    28681 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccacaaggg catatttcgt
    28741 cagcatgttt atattgcctc catgcgaaga agggactacc atccgagttg ttgaggttcg
    28801 gagtggcagc cccaggattg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28861 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcagcaaa
    28921 atatctgttt atttatacag taagtcctgt ttcggacttc tgttggcaac actcgttatt
    28981 gctcgtaaaa cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt
    29041 gccctaccat cctgacctga caaccggata tgcggggatt actccccgca caacggctac
    29101 ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt caccgtaaac
    29161 tcacacagcg agtctccgac cagaagcgta aatcccagca ccgtcaaaca cagcactatg
    29221 actgccgcaa gggcatattt cgtcagcatg tttatattgc ctccatgcga agaagggact
    29281 accatccaaa ttgttcgggt ttggagtggc agccccaggg ttgatagaaa tatctcctgg
    29341 ggcttcatct ttccagacct cagagtaaac ctgaaaccgg aaagcttcag gcacccgccg
    29401 ttatcttacc cttcctcaac aaaatggctg tttatttata cagtaaatcc tgtttcggac
    29461 ttctcactcc tcatttaatc aactgaagct gagcccgttt tttctgcatg cctcaatcgt
    29521 caaaattctt cgccatcagc cagctctgcc ataacttgcc acattgaaaa agtagccaaa
    29581 ttgcttcagt caccatcatc aaaaaaagaa acattccaga acttccgcat ctcgcccagc
    29641 cgctccgact tctatactga atagaaaacg ccaacataag agaaacctat gccattaccc
    29701 gattttcatg tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat
    29761 ccgccgggct atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaataag
    29821 atcacggccg gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat
    29881 gtttgccgtg atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg
    29941 caggcagccg cagaccatca tctggaaatt tgcggcggtg gcacacaccc gtttcagaaa
    30001 tggcagcgtc aggaggtatg cgataacgag cgctatcaac gaacgctgga aaactttggc
    30061 tatctcatcc agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc
    30121 gatgacgcca tttatttgct gcacggcttg tcacggtttg tgccgcactt tatcgccctt
    30181 tccgccgcgt cgccatatat gcaaggaacg gatacgcgtt ttgcctcctc acgaccgaat
    30241 attttttccg cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt
    30301 gaagccctgt ttcgctgttt gagttacacc acgatgatcg acagcattaa agatctgcac
    30361 tgggatattc gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg
    30421 ttaaccctta gccacgcggt aaatatggcg ggattaattc aggctaccgc ccactggtta
    30481 ctgacagaac gcccgttcaa acatcaggag aaagattacc tgctgtataa attcaaccgt
    30541 ttccaggcct gtcgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt
    30601 cgaccgctaa cggaagacac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa
    30661 attggtgcat cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa
    30721 gcgcagctga tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag
    30781 cattgtgaga tctgggccga tgactaagta gggaattgcc ttacagaaga atatccctga
    30841 caatggtgtt ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta
    30901 ccgcagcagg cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac
    30961 tggggctgac gctggcacaa aaactggtga ccatgttcca tctgatggat cttagctagc
    31021 tttacacttt attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc
    31081 tgcgctttat ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac
    31141 ttcgcggcat actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca
    31201 ctgcacgaaa cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct
    31261 tttggcccgg agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag
    31321 ctgcgcggcg tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag
    31381 cagatatacc cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg
    31441 tatcccggcg aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat
    31501 ctggtggttg atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga
    31561 aatctatagc gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac
    31621 gcaaaattac tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat
    31681 tccactaagg catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa
    31741 actctcctgc aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc
    31801 agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac
    31861 gaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac
    31921 gatgcctggc tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg
    31981 gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat
    32041 ctgcatgatg atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg
    32101 ctcggcgtgg cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc
    32161 ctcgatgcag aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta
    32221 ggtcatcaca gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac
    32281 atcaccttaa ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca
    32341 gattctcctg atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag
    32401 gagacgttat gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc
    32461 acaccacctc gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata
    32521 ttattttgca ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc
    32581 tttggttgca aatcataacc cagaataaac tcttccaccg tttagctttt accctgcagg
    32641 ggattatcgt caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata
    32701 ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt
    32761 tgctggatgt tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga
    32821 aagggatatt tcaggggatt aaactgatcg gcgcgatcct ggtcggcatt ttgatgatct
    32881 cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg
    32941 tgctgatgtt ggtatttaaa gatccgattc ttggtctggt ggccggtatt cagctttccg
    33001 cgaacgatat gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcagatggcg
    33061 cggtgatcga tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca
    33121 ctattcccac ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat
    33181 ctggcgggcg acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg
    33241 atgaagacga aatgcaacgt ctgaataaag cgcatttgtt aaagccttat ttaaccagcc
    33301 gccatcagga aattaatgag tggaatcgcc agcaaggttc tacggagtcg gtattaaacc
    33361 tgcgccgaat gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc
    33421 atccgcggat tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg
    33481 gtttaccgct cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca
    33541 ttcaggctga tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc
    33601 atcagtcgcc aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa
    33661 aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat
    33721 gccttatttc cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag
    33781 caggatcgct gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag
    33841 gaaggcaatg cagagatagt ttccgaacgg atagagcagc gccttaaact gtgtttcacg
    33901 cccctgacgt cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt
    33961 caacagcagc gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag
    34021 atagttgatt aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg
    34081 cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc
    34141 aaacagcatg cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag
    34201 aatgacgaag ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac
    34261 aaacgggcta ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag
    34321 tgaaccgatg taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct
    34381 tttatccgga tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga
    34441 gaacataatt accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc
    34501 gtagcgccag aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca
    34561 caggccaaag ccgatcatac cgatgattgc cagcacttta atcaacgcaa accagaactc
    34621 ggtttcgcca tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc
    34681 ggcagcccaa atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc
    34741 ggtcagctct gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa
    34801 gcccgcaaac ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc
    34861 ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc
    34921 gtagcccagc aatacagccg gacccgccat ctgaatcgcc ggaccaatgc caagaaacag
    34981 accagtacca attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg
    35041 atgaagcgtc ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac
    35101 gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt
    35161 cgcaagacac aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt
    35221 cggtgcagcc acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg
    35281 gtgatcatgc cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc
    35341 gttccccaca gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg
    35401 cgcacgcgca gtaccgcgcc gtgatacaac gcctcatcca gcttctgctc actgaatgtt
    35461 tgcggattat tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc
    35521 acgccaaatt cggcggcgac cccggcaagg gcaataaaac cggtgcccgt tgccacagaa
    35581 agatgaaagc ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg
    35641 ctgataatca gcaacgcttc gcccacccga cggaatgcca gatacaacag cacgaagatg
    35701 atcatcagtg tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg
    35761 aactgcccgg agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct
    35821 ttttgcaaat cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag
    35881 ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg
    35941 gccacgtctg ccagggtgat ttgctgcttc atcggcgtca ggatcggtag ctggcgcagt
    36001 gcctgcgggc tatcgcgcca gctttgcgga taacgcagat taatgggata acgggcaatc
    36061 ccttccaccg tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca
    36121 tccgccaccg tcataccgta acgcgcggct ttttcacggt taatctcaac gttgatatag
    36181 cgcccacctt ccagacgctc ggctagagca gaagccacgc ttggcaccgt tcgcgctact
    36241 tcttcaattt gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg
    36301 ccgatggggc ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc
    36361 cacagattcg ccagccccgg cagccgcacg gtgttatcca gttcctcaat gattttgtcc
    36421 atcgtcatgc caggccgcca ctgatcctgc ggcttaagct ggatggtcgt ttcaaccatc
    36481 tcgagcggag cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc
    36541 acttcaggta cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct
    36601 gcggaaatcc ccggcagcgt cgatggcata tacaacaagt cgccttcatt gatctgcggt
    36661 aaaaattccc cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc
    36721 accaacagcg tggttttcgg ccagtgcagt actttcagca atagaggatg ataaacacga
    36781 atcaaaaagc gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtac
    36841 cccatcagga tagggatcac tacgatcgcc agcagcgccg cgcccgccat cgcataggtt
    36901 ttagtgaacg ccaacgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg
    36961 ataaacgaca acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat
    37021 gcatcggtga tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc
    37081 tgccactctt ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc
    37141 gccccgacgg caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag
    37201 tgcatgacaa taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc
    37261 gagcgcacat gccagagaaa cagcgcacag actaccgcca ccacgataaa ctcttccagc
    37321 aacttgccgc tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta
    37381 actatctcca cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggag
    37441 gcgatcactt ctcgggcgtt tttgccggag cgcaggatca ccaccccgcc cgccacttcg
    37501 ccttcgccat tcagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca
    37561 acatcacgca gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag
    37621 tcgtcgagcg tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg
    37681 atcgacgaac cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggctaga
    37741 ctgatgccat attgcgccag gcgctgggga tcgataacca cctgatactc tttcaccacg
    37801 ccgcccaccg acgccacttc cgcaacgtca gggatggttt ttagctcata tttgagaaac
    37861 cagtcctgca atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca
    37921 tattcataga tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca
    37981 ggcagcttac cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga
    38041 tcggtaccat cttcgaaaat gacatacaca taagagtctc caaactgcga gaaaccgcgc
    38101 acagtcttcg cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt
    38161 tcaacgattt gcggtgcctg accgggatag ctggttttaa taatcacctg cacatcggag
    38221 agatccggca gcgcatccac tggcgtgtta atgatggtcc aggtgcccca gatgctcaga
    38281 aacaacgcgc ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca
    38341 atcattggtt attccctcaa tgcgcattgg tagcactttc agagcgcatc cgctccagtg
    38401 cgccagaaat attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac
    38461 cttccgccag accagagtgt aacgcggtga cgccttgcga tgcctggaaa acggcaacgc
    38521 gtttcggtac aaagcgtccg tcggcatcaa cggtaatcac ccgctgttcg ttgccggtat
    38581 caatcagcgc ctgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc
    38641 aggcgttcat gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca
    38701 gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt
    38761 tatccggtcg cgccggaacg gtgagcgtaa actgcgaggc atctttcacc agccaggcga
    38821 tagactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgctacca
    38881 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
    38941 tgggtgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat
    39001 ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg
    39061 taccgccggt ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg
    39121 tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct
    39181 tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga
    39241 cattcgccgg gaaactctgg gcaaaagtca gcggtccgcg cgtgacggta gccgttttca
    39301 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat
    39361 cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc
    39421 gcgtattggg gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt
    39481 tttctgcggg tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc
    39541 ccgcgatcat gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact
    39601 ggctgacttt aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca
    39661 ctttgtcgcc ggttttaatt tcactcattt tcgtctgcgg ggtgatggta aagcgcatag
    39721 tcatctccgg ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt
    39781 ccagatcaat accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca
    39841 tggtttcatg atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga
    39901 acattgcgac ttgcagtgct tttttcattt attctctcct ggagttaaaa attattgctg
    39961 ccaaccgccc cctaaggcgg tatacaaaga aatttcgtta acctgacggg cataattcag
    40021 gtcgagtaaa gtttgtcggg ttgcaaataa agaacgctcg gcatccagca cttccagata
    40081 acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc aaagtaattt gcagcgacgc
    40141 cagataacgc tgctgggcgc tgctttgatc gttcaggctt tgacgtaatg ccagcgcatc
    40201 tgccacttct ttaaaggcgt tctggatttt ctgttcataa ttcactaccg actgctgctg
    40261 gcgaatttcg gcgatatcca gattggcctg gttgcgtccg gcattaaaaa tggggatctc
    40321 aattttagga ataaaattcc acatcccgct gctagcgtta aataatgaag acagatcgct
    40381 gctggcggtt gatattccgc tggtcaggct gatagacggg aaaaatgccg cacgcgcagc
    40441 accaatatta gcattagccg ccattaacgc gtgttcagct tccataatat ccgggcgctg
    40501 caataagatt tgcgacgaca agcccgccgg taatttaacg ctttgcaggc tgccgctgtt
    40561 tactgtctgc gcttgcggca gtttgccgta gcttcccaat aacagttgca atgcattatt
    40621 cgcctgcgcc agttccccct gacgtttagc gatgtcactg cgggtacttt ctatcacccc
    40681 gcgagcctgt tccagcgcca gaacattgct gctaccggtc aacagttgtt tttcgacaaa
    40741 cgcatatgac tgctgataat tacgcagcgt ttcttcggct atttgcaatt gcgcatacgc
    40801 cagttgctga ttgaaatagc tttgcgcgac attagaaacc agcagaatat gcaccgcgcg
    40861 ctgagcttcc tcagtggcta aataattttg tcgctcggct tcgctcatgt tctttaagcg
    40921 accgaaaaaa tcgaggtcaa agctggcgtt aaggccggtc gaaaactccc gtgtcgtggc
    40981 ggtattccct ttaagattac cgctccagct gccgctgccc tcgccattga gctgtgggta
    41041 gcggtcggca tcggtcagac gatattgcgc ccgtgcctcc tgcactttca gcgtcgccat
    41101 gcgcaaatcc cggttattca ccagcgcctc gctaatcagc gtcttcacct gattatcaac
    41161 aaaaaaggtg cgccagccca cgttctggta gttatctgct gcgttaacca agccgttctg
    41221 gctgagtgag aactgctgcg gcacgggcat tgccgtacgc tgataatccg gtgccaatga
    41281 acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt ttacaaggag acataggctc
    41341 ataatttctg gtgattttat accgccaact ttactcgcca ggctctgatt ttccggtgac
    41401 aggaaaatga caaaattgtc attttgccaa taagcgattg ccatctgatc ccgctactct
    41461 agaattgccc gggcaacatg cggaggaaaa atgaaactgt tgattgtcga agatgaaaag
    41521 aaaaccggag aatacttgac caaagggtta accgaagccg gttttgtggt cgatttggcc
    41581 gacaacgggc tgaatggcta ccatctggcg atgaccggtg attatgacct gataatcctc
    41641 gatattatgc tgccggacgt gaacggctgg gatatcgtgc gcatgttgcg ctccgccaat
    41701 aaagggatgc cgattctgtt gcttaccgcg cttggcacca ttgaacatcg cgtcaagggg
    41761 ctggagttgg gggccgatga ctatctggtg aagccgttcg cttttgctga actgctggcg
    41821 cgggtgcgca ctctcctgcg gcgcggggcg gcggtgatta tcgaaagtca gtttcaggtt
    41881 gccgatctga tggtcgatct cgtcagccgc aaagtcaccc gcagcggcac gcgtatcact
    41941 ctgaccagta aagagtttac tctgctggag ttttttctcc gccatcaggg cgaagtgctg
    42001 ccccgctcgc ttatcgcctc gcaggtgtgg gacatgaatt tcgacagcga cactaacgcc
    42061 attgatgtgg cggtgaagcg gctacgcggc aaaatcgaca acgactttga gccgaagctg
    42121 attcagaccg tgcgcggcgt gggctatatg cttgaggtgc cggatggtca gtaagccatt
    42181 tcagcgcccg ttttcgctgg caacccgtct gacctttttt atcagcctgg ccaccatcgc
    42241 ggcgtttttc gcctttgcat ggatcatgat ccactcagtg aaagtgcatt ttgccgagca
    42301 ggatattaat gatttaaaag aaattagcgc cacccttgaa cgggtactta atcatcctga
    42361 cgagacgcaa gcccgacgct taatgacgct ggaagatatc gtcagtggtt attccaacgt
    42421 gttgatttcc ctggcagata gccacggcaa aacggtgtat cactcccccg gtgcgccgga
    42481 tatccgcgag tttacgcgtg acgccatacc cgataaagac gctcagggtg gcgaggtgta
    42541 tctcctttcc ggcccgacga ttatgatgcc aggccacggt cacgggcata tggaacacag
    42601 caactggcgg atgattaact tgccggttgg cccgttggtg gacggcaaac cgatttatac
    42661 gctctacatc gcgctttcga tcgattttca tcttcattac ataaatgatt tgatgaataa
    42721 acttattatg accgcatctg tcatcagcat cctgatcgtc tttatcgtgc tattggcggt
    42781 acataaaggt cacgcgccga tccgcagcgt cagccgtcaa atccagaata tcacctcgaa
    42841 agatctcgac gttcgccttg acccgcagac cgagcccatt gagctggaac agctggtact
    42901 gtcgttcaac catatgatcg agcgtattga ggatgtcttt acccgccagt ccaatttctc
    42961 agcggatatc gctcacgaaa ttcgcacgcc gattaccaat ctcatcacgc aaacggaaat
    43021 cgccctcagc cagtctcgca gccagaagga gctggaagat gtgctctact ctaatctcga
    43081 agagctgacg cgaatggcga aaatggtcag cgatatgctg tttctcgctc aggccgataa
    43141 caaccagcta atccccgaaa agaaaatgct caacctggcg gatgaagtcg gcaaagtgtt
    43201 cgattttttc gaggcgttag cggaagatcg cggcgtggag ttgcgatttg ttggcgacaa
    43261 atgtcaggtt gcgggcgatc cgctgatgct gcgtcgggcg ttaagcaacc tgctttctaa
    43321 tgccctgcgt tatacgccaa ccagagagac aattgtagtg cgctgtcaga cggtcgatca
    43381 ccaggtgcaa gttaccgtcg aaaaccccgg tacgcccatt gcgcccgagc acttaccgcg
    43441 attgtttgac cgtttctacc gcgttgaccc ttcccgccag cgaaaaggcg aaggtagcgg
    43501 cattgggctg gcgatagtga aatcgattgt tgtcgcgcat aaaggcacgg ttgcagtaac
    43561 gtcagatgcg cgggggacaa ggtttgtgat tatgctgccg gagagagagt gattattaag
    43621 ttcaaattca gggcgtggtg catcacctcc tggtattgtt gatcaacaaa ttagagaaaa
    43681 acttattaaa catatgaaat atcgaaggct tttatttttc aaagtttcac gagatgtttg
    43741 tatatgaaac ttagttaaat ctgtgtttct tccaattgta gatcacaatg ccctttctaa
    43801 attttcatcg ttataaacaa gccatctatc atataaagca atgaataatt taatcgcgat
    43861 taatcatcat taattgttat attattcact caatctattt gtcaagtgaa tttgataaat
    43921 tcctaattta aaatatccac tattgagcca gtatttaaca acgttaagat gtgcaatgtg
    43981 cagatcatgt tatcatgggt ggagcggtag catatatttc ttatgcaatc attcatttta
    44041 ataatgttaa ttggattatt tattgctgtg aataaaaata tattatgaat attggtttaa
    44101 ctttgcgttt ttgttactcg ttgtgatatg cgtacctgtc ctgaataacg ccaagaaaca
    44161 agaggttgag cttgtgatta tttttttgac tgaacagaca cccgtttctg taaagtaata
    44221 tttaaacaaa attctcacat aaccatagca ttttctgttc cttccctaat aacgtctgaa
    44281 gtcgtcccgc ttatcacatt atccgcagtt gtacggtacg gatcattatt cattttactg
    44341 gaggttgcta tgtcaaccgg attacgtttc acactggaag tggacggcct gccgccggac
    44401 gcttttgcgg tggtttcctt tcatctgaac cagtcactct cttcgctctt ttccctcgat
    44461 ctctccctgg tcagccagca gtttctctcc cttgaatttg cgcaggtgct ggacaaaatg
    44521 gcctacctga cgatatggca gggcgatgaa gtacagcgcc gggtgaaagg cgtggtgacc
    44581 tggtttgaac tcggggagaa cgacaaaaac cagatgctgt acagcatgaa ggtgcacccg
    44641 ccgctgtggc gtgccggtct gcgccagaac ttccgtatct tccagaacga ggacatcaaa
    44701 agcatcctcg gcacgatgtt gcaggaaaac ggggtgaccg aatggagtcc gctgttcagc
    44761 gagccgcatc cttcccgtga gttttgtgtc cagtacggtg agactgatta cgatttcctg
    44821 tgccggatgg cggcggagga aggcatcttc ttttatgagg agcatgctta caaaagtacc
    44881 gaccagagcc tggtgctgtg cgacacagtc cgccatctgc ccgaatcttt tgaaatccca
    44941 tggaacccga acacccgtac cgaggtgagc accctctgca tcagccagtt ccgctacagc
    45001 gcacaaatcc gcccttcttc cgtggtgacc aaagactaca cctttaaacg ccccggctgg
    45061 gccggacgtt ttgaacagga aggccagcac caggactacc agcgcacgca gtatgaagtg
    45121 tatgactacc ccggacgttt caagggtgct cacgggcaga actttgcccg ctggcagatg
    45181 gacggctggc gaaacaatgc agaaaccgcg cggggaatga gtcgctcgcc ggagatatgg
    45241 ccgggacgac gaattgtgct gacggggcat ccgcaggcga acctgaaccg ggaatggcag
    45301 gtggtggcaa gtgaactgca cggcgaacag ccacaggcgg tgccaggacg gcagggagcg
    45361 gggacggcgc tggagaacca ttttgcggtg atcccggcag acagaacatg gcgaccacag
    45421 ccgttgctga aaccgctggt cgacggcccg cagagcgctg tcgtgacagg accggcaggc
    45481 gaggaaatct tctgcgacga acatggtcgc gtgcgggtga agttcaactg ggaccgttat
    45541 aacccggcag accaagacag ttcgtgctgg atccgtgtgg cacaggcgtg ggcaggcacc
    45601 ggttttggcc acctggcgat accgcgtgtg ggtcaggaag tgattgtaga cttcctcaac
    45661 ggcgatccgg accagccgat cattatgggg cgcacctacc accaggaaaa ccgccccccc
    45721 cggcagcctg ccgggaacaa agacgcagat gaccatccgc tccaaaacgt acatgggcag
    45781 cggatttaat gagctgaagt ttgatgatgc gacagggaga gaacaggtct acatccacgc
    45841 gcagaagaac atggataccg aagtgctcaa cgaccgtacc accaccgtaa aacacgatca
    45901 ccgcgaaacc gtaaaaaatg accagacggt cacgatccag gaaggtaacc gccttcttac
    45961 ggtggaaaaa ggccacaaga tcaccggagt actgaaaggg tctttatctg aggatgtctt
    46021 tcaggacaga ggcacgattg ccggttcggt gcatgttgac gctgtaaaca atggtggcga
    46081 aggcgacggt atacaggctt atacggcgat taaggaaatt ttgctggccg tggaggaaag
    46141 caaaattgcg ctgacgccgg atagcattca gctacaggtc ggggaatcga cggtaatcag
    46201 gctgtcgaag gatggcatca ccatcgtggg cggttctgtt ttcatcaact gattcactca
    46261 gattatcagg gagtatttta tgcagttcac ttttaatgaa ggccacattc aactgccgtc
    46321 tcaatggcag gatcagtcaa tgcaggtcct ggtgtccaca gataacagtg gcatcaacct
    46381 ggtcattacc cgcgaagccg ttccacaggg aaccctgaca ccagagctgt accaggaaac
    46441 gctggcgctg taccagggca aacttgatgg gtacaccgaa catgcctgcc gggaaatcac
    46501 tctggcagag gccccggcct ggctgctgga ttattcctgg aacggtccgg aagatgaggg
    46561 gaatcagggg cggattagcc agatagccgt attccagcgg cgtggagata cgctgctgac
    46621 cttcactttc agcacctcct tatccctgaa gaacagtcaa aagacgatgc tgctggaggt
    46681 gataaagagc ttcaccccgt tgccaccgga gaatgacatc caaaaggacc agccgcgctg
    46741 acataaacga gggataacac atgagtgaag gaccaggcgg gccacaggga gcgaccgcag
    46801 gcggtacgct ggcaatgcga atgctgtcac agcaggcgat ggtcgccagc cagatgaaac
    46861 gggcagccaa cgacaaagcc attgcacaga tgttggcatc aaaaaagtct ggcccccccg
    46921 ccgccaggct gggcgatgaa attcagcaca agagtttttt gggggcgctg gcaggggccg
    46981 tactgggggc gatagtgacc atcgcagaag gttgcctgat tatggccgcc tgtgccaccg
    47041 gcccttatgc gctggttctg gtacctgcgc tgatgtatgc cagctataag gcgagtgatt
    47101 atgtggagga gaaacagaac cagcttgaat catggataaa cagcttttgt gacacggacg
    47161 gcgccatcaa taccggttct gaaaacgtaa aaattaacgg cgagctggcc gcacgtgcag
    47221 cggtcaccct tccccctcct cccccacctg gagcaatacc tgaagtccca cagggggaac
    47281 cctcatgggg tgatattgcc actgacctgc ttgaatcggc agcggaaaaa gcagtaccac
    47341 tggcgaaggc ctgggggaac gctgttatca ccctgacgga cagcaatgcc ggttttatgg
    47401 atcgcgtcag cgccggcgca tcgcttctgt ttcccgccgg tccggtatta atggagtttg
    47461 ccaccatggt gggcgggcgt ggcgaaatca aaaaagatgt ggatttcccg gaagccggtg
    47521 aggacacggc gctctgcgac aaggagaaca aaccaccgag gatagcccag ggcagcagca
    47581 acgtctttat caacaatcag cctgccgcgc gcaagggcga caaactggag tgcagtgcgg
    47641 cgatcgtggg aggttcgccg gacgtcttta ttggcggtga gcaggtcaca tatctggata
    47701 tccagccgga gttcccgccg tggcagagaa tgatcctggg gggaataacg atagccagct
    47761 atcttctgcc gccagcggga ctactgggaa aactgaagaa tctggcgaga ctcggcaaac
    47821 tgggaaactg ctggggaaaa gcgggaagct gctgggcgca aagctcggcg cgttgctggg
    47881 gaaaacaggt aagtcgttaa aaagtattgc caataaagtc atcagatggg taacagatcc
    47941 tgtcgatccg gtaaccggcg catactgcga cgaacgtacc gacttcaccc tgggccagac
    48001 cctccccctc tccttcaccc gtttccacag ttctgtactg ccgctgcatg gcctgaccgg
    48061 cgtgggctgg agcgactcct ggagcgaata cgcctgggtg cgtgaacagg gaaaccgggt
    48121 ggatatcatc agccagggag ccacgctgag atttgccttc gacggtgaca gtgatacggc
    48181 ggttaacccg tatcacgccc agtacattct gcgccgccgc gatgattatc tggagctgtt
    48241 cgacagggat gcactgagca gccgcttctt ttatgacgcc tttccgggaa tgcgtctgcg
    48301 ccacccggtg actgacgata ccagcgatga ccgcctggca cacagcccca gtgaccggat
    48361 gtacatgctg ggcgggatga gcgacaccgc cagcaaccgc atcacgtttg agcgcgacag
    48421 ccagtaccgg atcacgggtg tcagtcacac cgacgggatc cggcttaaac tgacgtacca
    48481 cgccagcggc tacctgaaag ccattcaccg cacggataac gacatacaga cgctggcgac
    48541 ctacgaacag gatgcgcggg ggcggctgac agaagcggat gcgcggctgg actaccacct
    48601 gttttatgag tacgacgctg cggaccggat catccgctgg tccgataacg accagacgtg
    48661 gagccgtttc acctacgatg aacagggccg gtgcgtgaat gtcaccgggg cggagggcta
    48721 ttacaacgcc acgctggact atggtgacgg ctgcaccacc gtgacggacg gcaagggcat
    48781 tcaccgttat tactatgatc ctgacggcaa tattctgcgg gaagaagcgc cggacggcag
    48841 caccaccatg tatgaatggg atgaattcca tcacctgctg gcccgccact cccctgccgg
    48901 gcgggtggag aaatttgaat acaacgccgc acacggtcag ttaagccgtt acacggcggc
    48961 agacggcgcg gagtggcagt accgctatga tgagcgcggc ctgctcagca acatcaccga
    49021 ccctgccggg cagacgtgga cacagcagtg tgatgaacgc ggcctgccgg taagtctggt
    49081 gtcgccacag ggcgaagaga cccggctggc atacaccgct caggggctgc tgtcgggaat
    49141 attccgccag gatgaacggc gtctgggcat agagtacgac caccacaacc ggccggaaac
    49201 actcaccgac gtgatgggcc gtgaacacca caccgaatac agcggtcacg acctgccagt
    49261 gaagatgcgc ggccccggcg gtcagtcagt gcggttacag tggcagcagc accataaact
    49321 gagcggcatt gaacgtgctg gaaccggcgc ggaaggattt cgctatgacc gccacggcaa
    49381 cctgctggcc tggacggacg gcaacggtgt tgtctggacg atggaatacg gtccgttcga
    49441 ttttccggtg gcgcgaacgg acggtgaagg ccaccgctgg cagtaccgct acgataaaga
    49501 cacgctgcaa ctgacagaag tcatcaaccc gcagggtgag tcatatcttt atgttctgga
    49561 taactgcggc cgggtgacgg aagagcgtga ctggggcggc gtggtctggc gttaccgcta
    49621 tgacgccgat ggcctctgta ccgccagggt taacggcctg gaggaaacca tcctctacag
    49681 ccgggacgcc gcaggccgcc tggcagaaat catctcgccg gaaggcaaaa cgcaatatgc
    49741 ctatgacaaa tccggcaggc tgacgggtat cttcagcccg gacggcacat cacagcgcac
    49801 cggctatgac gaacgcgggc gggtgaatgt caccactcag ggccgacggg ccattgaata
    49861 caactacccc gatgaacaca ccgtcatccg ctgtatcctg ccaccggaag atgaacgcga
    49921 cagacacccc ggtgaatccc tgctgaaaac cacgtaccgc tacaacgccg ccggagaact
    49981 gacagaggtc attctgccgg gggatgagac gctgacgttc agccgtgatg aggcgggacg
    50041 tgaagtgctc cggcacagta accggggttt tgcctgtgaa cagggctgga atgcagccgg
    50101 tcagcctgtc agtcagcgcg ccggattttt tccggcagaa gccacatggg acgggctggt
    50161 tccctcactg gtacgggagt accgttacga cagcgcgggt aacgtgtcag gcgtcaccag
    50221 ccgggaagat tacggacggg aaacacggcg ggagtatcgg ctggaccgga acggccaggt
    50281 cacggcggtg acagcctcag gcaccgggct gggctatggc gaaggcgatg agtcctatgg
    50341 ctatgacagc tgcggctacc tgaaggcgca gtctgcgggc aggcaccgga taagtgaaga
    50401 gactgaccag tatgccggag gccaccggct gaaacaggcc ggaaacacgc agtatgacta
    50461 tgacgccgca ggccggatgg tcagccgcac cagacaccgt gacggctacc gaccggaaac
    50521 agagcggttc cggtgggaca gccgggacca gctgaccggg tattgcagcg cacagggtga
    50581 gctgtgggaa taccgccacg acgccagcgg cagacgaaca gagaaacgct gcgaccggaa
    50641 gaaaatccgc ttcacgtatc tgtgggacgg cgacagtatt gcggaaatcc gggaataccg
    50701 cgatgataaa ctgtacagcg taaggcacct ggtgtttaac ggctttgagc tgataagcca
    50761 gcagttcagc cgggtacggc aggcgcatcc gtcggtggcc ccgcagtggg tgacgcggac
    50821 gaatcatgcg gtgagcgacc tgacgggccg cccgctgatg ctctttaaca gtgaaggaaa
    50881 gacggtctgg cgaccggggc agaccagcct gtgggggctg gcactcagcc tgcccgcaga
    50941 caccggctac ccggacccgc gcggggaact ggacccggaa gccgaccccg gcctgctgta
    51001 tgcaggacag tggcaggatg cggaatccgg gctgtgctat aatcggttcc ggtattacga
    51061 gccggaaacc ggaatgtacc tggtgagtga tccgctgggg ttgcagggcg gggagcagac
    51121 ttaccggtat gtgccgaatc cttgtgggtg ggttgatccg ctgggattgg ctgcaagttc
    51181 taaaatcagc agtttgatgg actatattgg cgatggtcgt cgtgttagtg ggcatacggg
    51241 tttcctggat ggggttcgtt tatcacgtag tcaaataa
//
LOCUS       DAZRVY010000018        49321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZRVY010000018 DAZRVY010000000
VERSION     DAZRVY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.018"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            292..1386
                     /gene="gcvT"
                     /locus_tag="ACHXOX_001060"
     CDS             292..1386
                     /gene="gcvT"
                     /locus_tag="ACHXOX_001060"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR7004598.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1410..1799
                     /gene="gcvH"
                     /locus_tag="ACHXOX_001061"
     CDS             1410..1799
                     /gene="gcvH"
                     /locus_tag="ACHXOX_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR7004599.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1918..4791
                     /gene="gcvP"
                     /locus_tag="ACHXOX_001062"
     CDS             1918..4791
                     /gene="gcvP"
                     /locus_tag="ACHXOX_001062"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR7004600.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDCFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5057..5800
                     /locus_tag="ACHXOX_001063"
     CDS             5057..5800
                     /locus_tag="ACHXOX_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7004601.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5857..7290)
                     /gene="bglA"
                     /locus_tag="ACHXOX_001064"
     CDS             complement(5857..7290)
                     /gene="bglA"
                     /locus_tag="ACHXOX_001064"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR7004602.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLNVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7335..7646
                     /gene="yqfB"
                     /locus_tag="ACHXOX_001065"
     CDS             7335..7646
                     /gene="yqfB"
                     /locus_tag="ACHXOX_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR7004603.1"
                     /translation="MQPNDITFFQRFLDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7810..8469
                     /locus_tag="ACHXOX_001066"
     CDS             7810..8469
                     /locus_tag="ACHXOX_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR7004604.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8665..9645)
                     /gene="ygfZ"
                     /locus_tag="ACHXOX_001067"
     CDS             complement(8665..9645)
                     /gene="ygfZ"
                     /locus_tag="ACHXOX_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR7004605.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9888..10154
                     /gene="sdhE"
                     /locus_tag="ACHXOX_001068"
     CDS             9888..10154
                     /gene="sdhE"
                     /locus_tag="ACHXOX_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR7004606.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10135..10542
                     /locus_tag="ACHXOX_001069"
     CDS             10135..10542
                     /locus_tag="ACHXOX_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR7004607.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10582..11103)
                     /gene="fldB"
                     /locus_tag="ACHXOX_001070"
     CDS             complement(10582..11103)
                     /gene="fldB"
                     /locus_tag="ACHXOX_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR7004608.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11215..12111
                     /gene="xerD"
                     /locus_tag="ACHXOX_001071"
     CDS             11215..12111
                     /gene="xerD"
                     /locus_tag="ACHXOX_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR7004609.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12136..12846
                     /gene="dsbC"
                     /locus_tag="ACHXOX_001072"
     CDS             12136..12846
                     /gene="dsbC"
                     /locus_tag="ACHXOX_001072"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR7004610.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12852..14585
                     /gene="recJ"
                     /locus_tag="ACHXOX_001073"
     CDS             12852..14585
                     /gene="recJ"
                     /locus_tag="ACHXOX_001073"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR7004611.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14676..15774
                     /gene="prfB"
                     /locus_tag="ACHXOX_001074"
     CDS             join(14676..14750,14752..15774)
                     /gene="prfB"
                     /locus_tag="ACHXOX_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR7004612.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15784..17301
                     /gene="lysS"
                     /locus_tag="ACHXOX_001075"
     CDS             15784..17301
                     /gene="lysS"
                     /locus_tag="ACHXOX_001075"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR7004613.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17344..17892)
                     /gene="idi"
                     /locus_tag="ACHXOX_001076"
     CDS             complement(17344..17892)
                     /gene="idi"
                     /locus_tag="ACHXOX_001076"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR7004614.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17947..18018
                     /locus_tag="ACHXOX_001077"
     CDS             17947..18018
                     /locus_tag="ACHXOX_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004615.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(18015..18140)
                     /gene="yqfG"
                     /locus_tag="ACHXOX_001078"
     CDS             complement(18015..18140)
                     /gene="yqfG"
                     /locus_tag="ACHXOX_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR7004616.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18142..19220)
                     /locus_tag="ACHXOX_001079"
                     /pseudo
     CDS             complement(18142..19220)
                     /locus_tag="ACHXOX_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19656..21575
                     /gene="ygfT"
                     /locus_tag="ACHXOX_001080"
     CDS             19656..21575
                     /gene="ygfT"
                     /locus_tag="ACHXOX_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR7004617.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21575..22063
                     /locus_tag="ACHXOX_001081"
     CDS             21575..22063
                     /locus_tag="ACHXOX_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR7004618.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22132..22482)
                     /gene="ghxQ"
                     /locus_tag="ACHXOX_001082"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22132..22482)
                     /gene="ghxQ"
                     /locus_tag="ACHXOX_001082"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22518..22832)
                     /locus_tag="ACHXOX_001083"
     CDS             complement(22518..22832)
                     /locus_tag="ACHXOX_001083"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR7004619.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22765..25704)
                     /locus_tag="ACHXOX_001084"
     CDS             complement(22765..25704)
                     /locus_tag="ACHXOX_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR7004620.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25701..26480)
                     /gene="ygfM"
                     /locus_tag="ACHXOX_001085"
     CDS             complement(25701..26480)
                     /gene="ygfM"
                     /locus_tag="ACHXOX_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR7004621.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26531..27859)
                     /gene="ssnA"
                     /locus_tag="ACHXOX_001086"
     CDS             complement(26531..27859)
                     /gene="ssnA"
                     /locus_tag="ACHXOX_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR7004622.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27862..30960)
                     /gene="ygfK"
                     /locus_tag="ACHXOX_001087"
     CDS             complement(27862..30960)
                     /gene="ygfK"
                     /locus_tag="ACHXOX_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR7004623.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31282..31860)
                     /gene="mocA"
                     /locus_tag="ACHXOX_001088"
     CDS             complement(31282..31860)
                     /gene="mocA"
                     /locus_tag="ACHXOX_001088"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR7004624.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31963..32733
                     /gene="yqeC"
                     /locus_tag="ACHXOX_001089"
     CDS             31963..32733
                     /gene="yqeC"
                     /locus_tag="ACHXOX_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR7004625.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32781..34406
                     /gene="yqeB"
                     /locus_tag="ACHXOX_001090"
     CDS             32781..34406
                     /gene="yqeB"
                     /locus_tag="ACHXOX_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR7004626.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34447..35379)
                     /gene="arcC"
                     /locus_tag="ACHXOX_001091"
     CDS             complement(34447..35379)
                     /gene="arcC"
                     /locus_tag="ACHXOX_001091"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR7004627.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35427..36812)
                     /gene="hyuA"
                     /locus_tag="ACHXOX_001092"
     CDS             complement(35427..36812)
                     /gene="hyuA"
                     /locus_tag="ACHXOX_001092"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR7004628.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36865..38076)
                     /locus_tag="ACHXOX_001093"
     CDS             complement(36865..38076)
                     /locus_tag="ACHXOX_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR7004629.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38134..39330)
                     /gene="dpaL"
                     /locus_tag="ACHXOX_001094"
     CDS             complement(38134..39330)
                     /gene="dpaL"
                     /locus_tag="ACHXOX_001094"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR7004630.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFPQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39388..40575)
                     /gene="ygeW"
                     /locus_tag="ACHXOX_001095"
     CDS             complement(39388..40575)
                     /gene="ygeW"
                     /locus_tag="ACHXOX_001095"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR7004631.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41054..42832
                     /locus_tag="ACHXOX_001096"
     CDS             41054..42832
                     /locus_tag="ACHXOX_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR7004632.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42872..43351)
                     /gene="xdhC"
                     /locus_tag="ACHXOX_001097"
     CDS             complement(42872..43351)
                     /gene="xdhC"
                     /locus_tag="ACHXOX_001097"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR7004633.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43348..44226)
                     /gene="xdhB"
                     /locus_tag="ACHXOX_001098"
     CDS             complement(43348..44226)
                     /gene="xdhB"
                     /locus_tag="ACHXOX_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR7004634.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44237..46534)
                     /gene="xdhA"
                     /locus_tag="ACHXOX_001099"
     CDS             complement(44237..46534)
                     /gene="xdhA"
                     /locus_tag="ACHXOX_001099"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR7004635.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46949..47704
                     /gene="actS"
                     /locus_tag="ACHXOX_001100"
     CDS             46949..47704
                     /gene="actS"
                     /locus_tag="ACHXOX_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR7004636.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47783..47852
                     /locus_tag="ACHXOX_001101"
     tRNA            47783..47852
                     /locus_tag="ACHXOX_001101"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47815..47817,aa:Gly,seq:ccc)
     gene            48057..>49321
                     /locus_tag="ACHXOX_001102"
     CDS             48057..>49321
                     /locus_tag="ACHXOX_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR7004637.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        11966 a        12175 c        13008 g        12172 t
ORIGIN      
        1 atttttttag ctcttaatat tgccttattc aaattagctt tctaatggca tcatctttat
       61 atagaaactg gtgtgttttt tggtttttta ttctgtcgcg attttggcgt tttttaacca
      121 taagctaatg tgatgatcaa ttttacctta tggttaacag tctgtttcgg tggtaagttc
      181 aggcaaaaga gaacgattgc gttggggacc gggagtggct ccgatgctgg gtttcgtggt
      241 gataatttca ccatgaaaag ttgtcagccc cgcttattca atgaggacaa gatggcacaa
      301 cagactcctt tgtacgaaca acacacgctt tgcggcgctc gcatggtgga tttccacggc
      361 tggatgatgc cgctgcatta cggttcgcaa atcgacgaac atcatgcggt acgtaccgat
      421 gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc tccgcggcag ccgcacccgg
      481 gagtttctgc gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc
      541 ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag atgacctcat cgtctactac
      601 tttactgaag atttcttccg cctcgttgtt aactccgcca cccgcgaaaa agacctctcc
      661 tggattaccc aacacgctga acctttcggc atcgaaatta ccgttcgtga tgacctttcc
      721 atgattgccg tgcaagggcc gaatgcgcag gcaaaagctg ccacactgtt taatgacgcc
      781 cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt
      841 attgccacca ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa
      901 gcggccgatt tctggcgtgc gctggtggaa gcgggtgtta agccatgtgg cttgggcgcg
      961 cgtgacacgc tgcgtctgga agcgggcatg aatctttatg gtcaggagat ggacgaaact
     1021 atttctcctt tagccgccaa catgggctgg actatcgcct gggaaccggc agatcgtgac
     1081 tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa actggttggt
     1141 ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg
     1201 cagggcaacc agcatgaagg cattatcacc agcggtactt tctccccgac gctgggttac
     1261 agcattgcgc tggcgcgcgt gctggaaggt attggcgaaa cggcgattgt gcaaattcgc
     1321 aaccgtgaaa tgtcggttaa agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc
     1381 gcgtgattta cttttttgga gattgattga tgagcaacgt accagcagaa ctgaaataca
     1441 gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg
     1501 aacatgctca ggagctgtta ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa
     1561 cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt
     1621 atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc actgagcgat tccccggaac
     1681 tggtgaacag cgaaccgtat gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa
     1741 gcgaactgga atcactgctg gatgcgaccg catacgaagc attgttagaa gacgagtaac
     1801 ggctttattc ctcttctgcg ggagaggatc agggtgagga aaatttatgc ctcaccctca
     1861 ctctcttcgt aaggagagag gttcacaatt cactgcacgt ttcaggaacc atcgctcatg
     1921 acacagacgt taagccagct tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg
     1981 gacgccgcgc aacagcaaga aatgctgaat gccgttggcg cacaatcgtt aaacgcgctg
     2041 accggccaga ttgtaccgaa agatattcag cttgcgaccc cgccgcaggt tggcgcaccg
     2101 gcgaccgaat acgccgcact ggcagaactc aaggctattg ccagtcgcaa taaacgcttc
     2161 acgtcttaca tcggcatggg ttacaccgcc gtgcagctac cgccggttat cctgcgtaac
     2221 atgctggaaa atccgggctg gtataccgcg tacactccgt atcaacctga agtctcccag
     2281 ggccgccttg aagcactgct caacttccag caggtaacgc tggatttgac tggactggat
     2341 atggcctctg cttctcttct ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg
     2401 aaacgcgtca gcaaactgaa aaatgccaac cgcttcttcg tggcttccga tgtgcatccg
     2461 caaacgctgg atgtggtccg tactcgtgcc gaaacctttg gttttgaagt gattgtcgat
     2521 gacgcgcaaa aagtgctcga ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc
     2581 actaccggtg aaattcacga ctacactgcg cttattagcg aactgaaatc acgcaaaatt
     2641 gtggtcagcg ttgccgccga tattatggcg ctggtgctgt taactgcgcc gggtaaacag
     2701 ggcgcggata ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc
     2761 ccacacgcgg cattctttgc ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt
     2821 atcggtgtat cgaaagatgc agctggcaat accgcgctgc gcatggcgat gcagactcgc
     2881 gagcaacata ttcgtcgtga gaaagcgaac tccaacattt gtacttccca ggtactgctg
     2941 gcaaacatcg ccagcctgta tgccgtttat cacggcccgg ttggcctgaa acgtatcgct
     3001 aaccgcattc accgtctgac cgatattctg gcggcgggcc tgcaacaaaa aggtctgaaa
     3061 ctgcgccatg cgcactattt cgacaccttg tgtgtggaag tggccgacaa agcgggcgta
     3121 ctgacgcgtg ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa cgccgtgggg
     3181 atcacccttg atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag cgtgctgctg
     3241 ggcgataacc acgggctgga gatcgacacg ctggacaaag acgtggctca cgacagccgc
     3301 tctatccagc ctgcgatgct gcgcgacgac gaaatcctca cccatccggt gtttaatcgc
     3361 taccacagcg aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa agatctggcg
     3421 ctgaatcagg cgatgatccc gctgggttcc tgcaccatga aactgaacgc cgccgccgag
     3481 atgatcccaa tcacctggcc ggaatttgcc gaactgcacc cgttttgccc gccggaacag
     3541 gctgaaggtt atcagcagat gattgcgcag ctggctgact ggctggtgaa actgaccggt
     3601 tacgacgccg tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg
     3661 gcgattcgtc attatcatga aagccgcaac gaagggcatc gcgatatctg cctgatcccg
     3721 gcttctgcgc acggaactaa ccccgcttct gcacatatgg caggaatgca ggtggtggtt
     3781 gtggcgtgtg ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa agcggaacag
     3841 gcgggcgata acctctcctg tatcatggtg acttatcctt ctacccacgg cgtgtatgaa
     3901 gaaacgatcc gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat
     3961 ggcgcgaaca tgaacgccca ggttggcatc acctcgccgg gctttattgg cgcggacgtt
     4021 tcgcacctca acctgcataa aactttctgc attccgcacg gcggtggtgg tccgggtatg
     4081 ggaccgatcg gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa
     4141 atcgaaggca tgttaacccg tcagggcgca gtttctgcgg caccgttcgg tagcgcctct
     4201 atcctgccaa tcagctggat gtacatccgc atgatgggcg cagaagggct gaaaaaagca
     4261 agccaggtgg caatcctcaa cgccaactat attgccagcc gcctgcagga tgccttcccg
     4321 gtgctgtata ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg
     4381 ctgaaagaag aaaccggcat cagcgagctg gatattgcca agcgcctgat cgactacggt
     4441 ttccacgcgc cgacgatgtc gttcccggtg gcgggtacgc tgatggttga accgactgaa
     4501 tctgaaagca aagtggaact ggattgcttt atcgacgcga tgctggctat ccgcgcagaa
     4561 attgaccagg tgaaagccgc tgtctggccg ctggaagata acccgctggt gaacgcgccg
     4621 cacattcaga gcgaactggt cgccgagtgg gcgcatccgt acagccgtga agttgcggta
     4681 ttcccggcag gcgtggcaga caaatactgg ccgacggtga aacgtctgga tgatgtttac
     4741 ggcgaccgta acctgttctg ctcctgcgta ccgattagcg aataccagta attcactgat
     4801 tcgactattt tctaaaggcg cttcggcgcc tttttagtca gatgacaaag tacaaaagtg
     4861 ctcagacagt cccctcgccc ctttggggag agggttaggg tgaggggaac aggccggcac
     4921 tggcgcgaat atttaccctc accccggccc tctccctgaa agggcgaggg gaaaagcgtg
     4981 ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa cgaattaatt
     5041 tttctcggaa aaacaaatgg ctatagcact tgtgaccgat ggcagtcgcg gcatcgggcg
     5101 ggcaactgca ttactgttgg cgaaagaaga gtatacggtg gcggttaatt atcaacaaaa
     5161 tctccatgcg gcgcaggaag tggtgaactt aatcacgcaa gccggtggca aggcattcgt
     5221 gctccaggcg gatatcagcg acgaaaatca ggtcatcgca atgtttacag caatcgatca
     5281 gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta cccagtgcac
     5341 cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaaca tgacgggata
     5401 ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg
     5461 cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg aatatgttga
     5521 ttacgcggca tcgaaagggg cgattgatac gttaaccacc ggactatcgc tggaagtcgc
     5581 cgcgcagggg gtccgcgtta actgcgtgcg gccagggttt atttttaccg aaatgcacgc
     5641 cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg
     5701 tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata aagcctctta
     5761 cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag ttgtctgacc
     5821 ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc cgttgctggt
     5881 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
     5941 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtggtgaa
     6001 cgatacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacgc catcgtaagt
     6061 caccgctttt ttcatctctt cgatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
     6121 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
     6181 cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
     6241 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt cgaaaccaga
     6301 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
     6361 aagataatcg caggtgcctt cgcgcagtac attgagatcg ccgtcttcca ttttgatgtt
     6421 aaatccgcga cgctcccact cgttcaacac ataggacggg taatagccgc gtagctgcac
     6481 atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
     6541 acagcagtag ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
     6601 aatgcgacgc gcagctttca ccgccagggc gctggcgaca aactggtgat gcagcacctg
     6661 atacatcgtc tcttccgggt tctcatgctc ggtataaacc acgccggagc agcagtaacc
     6721 gaacagcggt gcacgccagt tacgctggtt gttaatttcg ttgaaggtca tccagtattt
     6781 gactttatgc ttatagcgtt caaacaccac ttccgcgaaa cggacaaaga aatcaaccac
     6841 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
     6901 gagggtgatc accggttcga tgttgtattt cagcagttca tcgaacatat catcgtagaa
     6961 cttcagccct tcttcgtttg gctgagcttc atcgcctttt ggaaaaatgc gcgtccaggc
     7021 tatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
     7081 gtgaccataa aaatcaacgg cttcatggtt tggatagtat ttgcctggca agacttcttt
     7141 ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggccc
     7201 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaactgcgc cgccccataa
     7261 gaaatctttc tgtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta
     7321 caggaaatag taccatgcag ccaaacgaca tcactttttt tcaacgtttc ctggatgaca
     7381 ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg
     7441 gtgatgtgct tcgtgtcggg cgttttgaag atgacggtta tttttgcacg attgaagtca
     7501 ccgcaacctc aaccgtaacg ctggatacgc tgacggaaaa acatgcagag caggaaaata
     7561 tgaccctgac tgaactgata aaggtcattg ccgacatcta tcccggtcag acacagtttt
     7621 atgtgattga atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttcgcat
     7681 ttaatttcat gatttttagg agattagtct caaatcagaa tttattttag ctaacaggtg
     7741 ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg
     7801 gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt
     7861 gccaacagcg tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta
     7921 ctggttcagg cggtggatct caatgccagc gccacagcga taaccagtta cagcctctat
     7981 ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa
     8041 cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga
     8101 acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt
     8161 gttatctgga gcctggcatt gctgggtatt ctgtttaaac tgaccatcgc gcaccgattc
     8221 aaaattttat ctctggtgac ctatctggcg atgggctggc tgtcgctggt ggtaatttat
     8281 gaaatggcag ttaagctcgc ggcgggcagc gttaccttat tggcggtagg cggcgtggtt
     8341 tattcgctcg gagtgatttt ctatgtctgc aaacgcattc cctacaatca tgccatctgg
     8401 cacggcttcg tgctcggcgg cagtgtgtgc cactttctgg cgatctattt gtatattggg
     8461 caggcgtaat gggtatgcct ttgtaggcct gaaaagacgc gtcagcgtcg catcaggcat
     8521 cggagcacaa ctgccggatt cggcgtgaac gccttatccg atctacgggc ctcgtccctg
     8581 ttgtgataaa ggcatcaaat cccagacatg ataagacgcg tcagcgtcgc atcaggcgat
     8641 aaatgcctga tgcgataaag agatttactc ttcgagcgaa tacggcagcg gctcaatatg
     8701 caatgtattc gcatcgtcgc gtacgcggaa gatgctatcc ggttccatat cgttattcat
     8761 gacgacctgt accacgacct gaccatcttc cagttttacc gcagccagca ccgtaccggt
     8821 acgacgccag ttctcgccca ttttcagctc taagtcttca ccagcttccg gcagtcggct
     8881 ggcgctacct gccagcaacc agagcgcacg tttattggca ccacggaatt ttgctcgcgc
     8941 caccatctct tgtccggtat aacagccttt cttaaagctg ataccgccca gcgcctggag
     9001 attggtcgcc tgtgggataa actgcccgct gttggcggca tcaatcaccg ggaaacccgc
     9061 ttcaatgttt aatgccagcc actgttggct attgttcagt tccgcttcac cgcgcagttt
     9121 atcggtcagc atattagcag tagcttcatc ggttacgatc aggaaacgtt ctgccgggta
     9181 ttcaaaccat agcaaagtgg tcgcgccttc tttaactacc tgtttttctt tcgaaggcag
     9241 ttcactaaag atatttgcca gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac
     9301 atgctcgtcg tctggcgcga tggtcacttt agagaacacc gcatattttt tcagttcagt
     9361 cagctgcggt tcacgcacgc tgcgccgttc aatccacgca aagccatcgc cgtcgcggaa
     9421 cagacgtaaa ttgctccaca ttttgccttt ggcgtcgcaa tgggcggcga gcaggtgctg
     9481 atcttctgcc atctggctga catctgctgt cacctgaccc tgcatatatt tttcgctgtc
     9541 cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg
     9601 ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa gccataatag agtcctgagt
     9661 tgcttaacgg cgtattaatg actaatggta aaagagctat gaggcaatgc aagtgctttc
     9721 actacgcgat ctgtgccttc tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt
     9781 gtgaattaat ttcgtgatcg cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa
     9841 acacgttaca atagcggggt actggatttt cgtgagacag gaagaagatg gacattaaca
     9901 acaaagcccg cattcattgg gcatgccgcc gtggtatgcg cgaactcgat atttcaatca
     9961 tgccgttttt cgaacatgag tacgacagct taagcgatga cgaaaaacgc atctttattc
    10021 gtctgctgga atgtgacgat ccggacctgt ttaactggct gatgaatcac ggtaaaccgg
    10081 ccgatgcaga actggaaatg atggtccgac tcatccagac acggaaccgg gaacgtggtc
    10141 ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt ggctttcctt gctgattcat
    10201 gggctggttg ccgctgttat tttactcatg ccctggccac tcagttacac cccgttatgg
    10261 atggtgttac tttcgctggt ggtgtttgat tgcgttcgca gccagcggcg tattaatgct
    10321 cgccaggggg aaattcgctt gttgatggac gggcgtttgc gttggcaagg gcaggagtgg
    10381 agcatcgtca aagcaccgtg gatgattaag agcggcatga tgctgcgttt acgttctgat
    10441 ggcggtaaac ggcaacattt atggctggca gccgacagca tggacgaagc cgaatggcgg
    10501 gatttacggc ggattttgtt gcaacaagag acgcaaagat aaagatgacg gcaaagcata
    10561 tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc atttcgttga ggatttgctc
    10621 gcaccagctc tgaatacgct cgtcgctaag gtcatactgg ttagtttcat ccagcgccag
    10681 acccacgaac agttgcccgt cagcaatcac cggtttcggg ctggtaaatt catatccttc
    10741 cgttggccag tagccgacga acttcacgcc tttggtcgag agtttgtcat gcagcatacc
    10801 gagcgcatcg aggaaccact cgccgtatcc cagttgatcg ccaagcccat acagcgcaac
    10861 aattttacct tcaaggttca ggtcgtcgag ctgatcccag acggcttccc agtcttcctg
    10921 gatttcacca aaatcccagg tcgggatacc cagaatgagc acatcgtact gctccattaa
    10981 tttcggggag tcgtccttga ggttatgtaa ggtcaccagt tctgggccga taatatcgcg
    11041 gattttttct gccgccattt cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt
    11101 cataacgtaa gcttctcgat tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt
    11161 agtcacggca caataaggca taatggatca aatgagtgac cataaggggc gcaagtgaaa
    11221 caggatctgg cacgcatcga gcagtttctt gatgctctgt ggctggaaaa aaatctggct
    11281 gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac
    11341 cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc aggcattact ggcagaacgg
    11401 ctggagggcg ggtataaagc caccagctca gcgcgtttgc tgagtgcagt gcgccgattg
    11461 ttccagtatc tttatcgcga aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca
    11521 ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag cgcaggtcga acgtttatta
    11581 caggcgccat taattgatca gccactggag ctacgcgata aagccatgct tgaagtgttg
    11641 tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga caatgagtga tatcagcctg
    11701 cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag agcgcctggt accgttaggt
    11761 gaagaggcgg tttactggct ggaaacctat ctggaacatg ggcgtccgtg gctgctgaat
    11821 ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc agcagatgac gcgacagacc
    11881 ttctggcacc gcattaaaca ttatgccgtg ctggcgggta tcgacagcga aaagctgtca
    11941 ccgcatgtgt tgcgtcacgc ttttgccact catttattaa atcatggtgc ggatttacgc
    12001 gtggtgcaga tgctactggg ccacagcgat ctttccacca cgcaaattta tacgcatgtc
    12061 gctaccgagc gtctgcggca acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa
    12121 agaacgggaa gatttatgaa gaaaggtttt atgttgttta ctttgttagc ggcgttttca
    12181 ggttttgctc aggctgatga cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa
    12241 agcagcgata ttcagcccgc gcccgtagct ggcatgaaga cagttctgac taacagcggc
    12301 gtgttgtaca tcaccgatga tggtaaacat atcattcagg ggccaatgta tgacgttagc
    12361 ggcacggctc cggtcaatgt caccaataag atgctgttaa agcagttgaa tgcgcttgaa
    12421 aaagagatga tcgtttataa agcgctgcag gaaaaacacg tcatcaccgt gtttactgat
    12481 attacctgtg gttactgcca caaactgcat gagcaaatgg cagactataa cgcgctgggg
    12541 atcaccgtgc gttatcttgc tttcccgcgc caggggctgg acagcgatgc agagaaagaa
    12601 atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt
    12661 aaaagcgtcg caccagccag ctgcgacgtg gatattgccg accattacgc acttggcgtc
    12721 cagcttggcg ttagcggtac tccggcagtt gtgctgagca atggcacact tgttccgggt
    12781 taccagccgc cgaaagagat gaaagaattc ctcgacgaac accaaaaaat gaccagcggt
    12841 aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg tgcagtcgat gaaacggcag
    12901 acttgcccgc tgaattgcct cccttgctgc gccgtttata cgccagccgg ggcgtgcgca
    12961 gtgcgcaaga actggaacgc agtgttaaag ggatgctgcc ctggcagcaa ctgagcggcg
    13021 tcgaaaaggc cgttgagatc ctttacaacg cctttcgcga aggaacgcgg attattgtgg
    13081 tcggtgattt cgacgccgac ggtgccacca gcacggctct aagcgtgctg gcgatgcgct
    13141 cgcttggttg cagcaatatc gactacctgg ttccaaaccg tttcgaagac ggttacggct
    13201 taagcccgga agtggtcgat caggcccatg cccgtggtgc gcagttaatt gtcacggtgg
    13261 ataacggtat ttcctcccat gcgggggttg aacacgctcg ctcgttgggc attccggtta
    13321 ttgttaccga tcaccatttg ccgggcgaaa cattacccgc ggcggaagcg atcattaacc
    13381 ctaacttgcg cgactgtaat ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt
    13441 atctgatgct ggcgctgcgc acctttttgc gcgatcaggg ctggtttgat gagcgcggca
    13501 tcgcaattcc taacctggca gaactgctgg atctggtagc gctggggaca gtggcggacg
    13561 tcgtaccgct ggacgctaat aatcgcattc tgacctggca ggggatgagt cgcatccgtg
    13621 ccggaaagtg ccgtccgggg attaaagcgc tgcttgaagt ggcaaaccgt gatgcacaaa
    13681 aactcgccgc cagcgattta ggttttgcgc tggggccacg tctcaatgct gccggacgac
    13741 tggacgatat gtccgtcggt gtggcgctct tgctgtgcga caacatcggc gaagcgcgcg
    13801 tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc
    13861 aggttgaagc cctgaccctg tgcgagaaac tggagcgcag ccgtgacacg ctacccggcg
    13921 ggctggcaat gtatcacccc gaatggcatc agggggttgt cggtattctg gcttcccgta
    13981 ttaaagagcg ttttcaccgt ccggttatcg cctttgcgcc cgcgggtgat ggtacgctga
    14041 aaggttctgg tcgctccatt caggggctgc atatgcgtga tgcgctggag cgattagaca
    14101 cactctaccc tggcatgatg ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc
    14161 tggaagcgga taaattcgaa ctctttcaac aacggtttgg cgaactggtt actgagtggc
    14221 tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa
    14281 tgactatgga agtggcgcag ctgctgcgcg atgctggccc gtgggggcag atgttcccgg
    14341 agccgctgtt tgacggtcat ttccggctgc tgcaacagcg gctggtgggc gaacgtcatt
    14401 tgaaagtcat ggtcgaaccg gtcggcggcg gtccgctgct ggatggtatt gcttttaatg
    14461 tcgataccgc cctctggccg gataacggcg tgcgcgaagt gcaactggct tacaagctcg
    14521 atatcaacga gtttcgcggc aaccgcagcc tgcaaattat catcgacaat atctggccaa
    14581 tttagcgtca tcttctctat aaaaaagagc gtggattggg tacaatcccg ctcttatcac
    14641 cgcattttga ctagctcaat aaaagaaatc agaccatgtt tgaaattaat ccggtaaata
    14701 atcgcattca ggacctcacg gaacgctccg acgttcttag ggggtatctt tgactacgac
    14761 gccaagaaag agcgtctgga agaagtaaac gccgagctgg aacagccgga tgtctggaat
    14821 gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac
    14881 accctcgacc aaatgaaaca ggggctggaa gatgtttctg gtctgctgga actggctgta
    14941 gaagctgacg acgaagaaac ctttaacgaa gccgttgctg aactcgacgc cctggaagaa
    15001 aaactggcgc agcttgagtt ccgccgtatg ttctctggcg aatatgacag cgccgactgc
    15061 tacctcgata ttcaggcggg gtctggcggt acggaagcac aggactgggc gagcatgctt
    15121 gagcgtatgt atctgcgctg ggcagaatcg cgtggtttca aaactgaaat catcgaagag
    15181 tcggaaggtg aagtggcggg tattaaatcc gtgacgatca aaatctccgg cgattacgct
    15241 tacggctggc tgcgtacaga aactggcgtt caccgcctgg tgcgtaagag cccgtttgac
    15301 tccggcggtc gtcgccacac gtcgttcagc tctgcgtttg tttatccgga agttgatgat
    15361 gatattgata tcgaaatcaa cccggcggat ctgcgcattg acgtttatcg cgcgtccggc
    15421 gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc gtattaccca catcccgacc
    15481 gggatcgtga cccagtgcca gaacgaccgt tcccagcaca agaacaaaga tcaggccatg
    15541 aagcagatga aagcgaagct ttatgaactg gagatgcaga agaaaaatgc cgagaaacag
    15601 gcgatggaag ataacaaatc tgacatcggc tggggcagcc agattcgttc ttatgtcctt
    15661 gatgactccc gcattaaaga tctgcgtacc ggggtagaaa ctcgcaacac gcaggccgtg
    15721 ctggacggca gcctggatca atttatcgaa gcaagtttga aagcagggtt atgaggaacc
    15781 aacatgtctg aacaacacgc acagggcgct gacgcggtag tcgatcttaa caatgaactg
    15841 aaaacgcgtc gtgagaagct ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat
    15901 ttccgtcgcg atcatacctc tgaccaattg cacgcagaat tcgacggtaa agagaacgaa
    15961 gaactggaag cgctgaacat cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt
    16021 atgggtaaag cgtctttcgt taccctgcag gatgtgggtg gccgcattca gctgtacgtt
    16081 gcccgtgacg atctgccgga aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc
    16141 gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa ccggcgaact gtctatccac
    16201 tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc tgccggataa attccacggc
    16261 ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg atctcatctc taacgatgaa
    16321 tcccgtaaca cctttaaagt acgctcgcag atcctctctg gtattcgcca gttcatggtg
    16381 aaccgcggct ttatggaagt tgaaacgccg atgatgcagg tgatcccagg cggtgccgct
    16441 gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg acatgtacct gcgtatcgcg
    16501 ccggaactgt acctcaagcg tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac
    16561 cgtaacttcc gtaacgaagg tatttccgta cgtcataacc cagagttcac catgatggaa
    16621 ctctacatgg cttacgcaga ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt
    16681 actctggcac aggatattct cggtaagacg gaagtgacct acggcgacgt gacgctggac
    16741 ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga tcaagaaata tcgcccggaa
    16801 accgacatgg cggatctgga taacttcgac tctgcgaaag cgattgctga atctatcggt
    16861 atccacgttg agaagagctg gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg
    16921 gcagaagcac atctgattca gccgaccttc attactgaat atccggcaga agtttctccg
    16981 ctggcgcgtc gtaacgacat taacccggaa atcacagacc gctttgagtt cttcatcggt
    17041 ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc
    17101 ttcctggatc aggttgccgc gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa
    17161 gattacgtta ccgcactgga acatggctta ccgccgacag caggtctggg gattggcatc
    17221 gaccgtatgg taatgctgtt caccaacagc cataccatcc gcgacgttat tctgttcccg
    17281 gcgatgcgtc cggtaaaata agcattacgt tatgctcaca accccggcaa atgtcggggt
    17341 tttttaattg tgctgcgcga aagcagacaa caattttctt gcttcactat tggctgcctg
    17401 catcaccatc cacggactga acgcccacgg tgtggcatca ataccgtgta atacatctgc
    17461 taaatcacac cattgataat ccatcacttc atcatcgttg atctgtaacg cactggtggt
    17521 gcgtgcggca aataccggac acacttcatt ttccacaatg ccattcggat cggtggcgcg
    17581 atagcgaaag tcaggataga tagattcagg aggcgtaatt tccacgccaa gctcataacg
    17641 gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga
    17701 gttagtccac acgccaggcc acgctttttt gctcagtgcg cggcgggtaa ctaataattg
    17761 tcctttggca ttaaacagcc aactggagaa cgcgagatgt aagcgggtgt ctgccgtgtg
    17821 tgcggcatac ttttccagcg tacccgtggg aactccctgt gcattcaata aaatgacgtg
    17881 ttccgtttgc ataatttctc acatgtaatt ctgatcactc atcattatag ataattgaag
    17941 agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc ccgttttgag cggatgccga
    18001 caggtaaaaa ccatttaaaa aagtagcatc atatatttga agcgactttc aatggccatt
    18061 gcaacacagt cagaaatgac aggaatagag agagtaaaaa gcaacagcag actgaatata
    18121 acgcgcatta aaaaattcat attattctcc atgctcattt ttcttcagct tgcgcatcag
    18181 aagccacata cgtactgtcc tgacttttaa atctttatca gagacggatt ccttcaccag
    18241 gtcagcatga tgctggtagc cattaaagaa gacattcagc acaacggcgc taagggttgc
    18301 gagcataatg ccgctatgca gcaacggttg cagtacggcc ggtaacttag aaaagaaatc
    18361 gtgagagagc gtcggagtca tgccaacgcc gagactgatc gccacaatat agaggttgta
    18421 acggttggtg gtgtagttac agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc
    18481 gaacatcacc agcccagcac cgcccagcac aaattgcgga atggaggcga ccagcaccgc
    18541 catttttggc accatgccaa acaggattaa tataattccc gaagaaatac acacccagcg
    18601 gctataaatc ctttcgcata gcgagtaatg agcaagataa aaattaagac agcaaaggag
    18661 atacctaaat aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca
    18721 atacccacct gaatgatgct aagcccaata gaagtaataa ccacaccggt aaccagtggc
    18781 gggaataaag gcatcaagcg accaataaat ggcgctaata atgtggtgat aaaacccgcg
    18841 gcgatagtgg caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc
    18901 attggtgtta cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag
    18961 cggccgatac cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta
    19021 atgagcatcg caatagcatc ttttgagagg cccagtcggt caccaatcat aagaggaaca
    19081 gcgactgcac ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct
    19141 ggtgataata tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt
    19201 tgggaatcta tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg
    19261 tttacgttaa tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg
    19321 ggagttagag acaggaattg cggaggcaat cacataatcg tgcttaaacc acgaaaatga
    19381 caagagaacc tgacaatgga attatctgta attttctcat attgatattt cattaatcat
    19441 tctggcatgt cgcactctag catttaatcg tttttatctg gatagcgctc ttttgatcgg
    19501 caatgttgaa ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa
    19561 aattataatt aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt
    19621 gagaagacat aactcattaa atgaaaggga tgcaaatgaa taagtttatc gctgctgaag
    19681 ctgcggaatg tataggctgt catgcttgtg aaattgcctg tgcggtggca cacaatcaag
    19741 aaaactggcc gctgagtcac agtgactttc gaccgcgtat ccacgttgta gggaaaggcc
    19801 aggctgcgaa tccggtggcc tgccatcact gcaacaatgc cccttgtgtt acggcttgtc
    19861 cggttaatgc tctgactttc cagtccgata gcgtacaact ggacgagcaa aaatgtattg
    19921 gctgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc gatacgattg
    19981 cgcagaaatg cgacctttgt aaccagcgca gttccggcac gcaagcctgt attgaagtct
    20041 gcccaacaca ggcgttacga ctgatggacg ataaagggtt acagtggata aaggtggctc
    20101 gccagcgcaa aacggcagca ggaaaagcgt catcagacgc tcagccatct cgcagtgcag
    20161 cgttgctccc cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt gaacggaaaa
    20221 cccactttgg cgaaatctat tgcgggctgg atccactgca agcgacttat gagagtgacc
    20281 gctgtgttta ttgtgccgaa aaagctaact gcaactggca ttgcccgctg cataacgcta
    20341 ttccggatta catccgtctg gtacaggaag gaaagattat tgaagcggca gaactttgcc
    20401 accagaccag ttccttacca gaaatctgcg gcagggtatg cccacaggac cgtctttgtg
    20461 aaggcgcatg tactttgaaa gatcactctg gcgcagtctc tatcggtaat ctggaacgct
    20521 acatcaccga taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc
    20581 gtatcgaaaa agtggcggtg attggcgctg ggccagcagg gttagggtgt gctgatattc
    20641 tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc
    20701 tgacttttgg cattcctcct ttcaaactcg ataaaacggt attaagccag cggcgagaga
    20761 tattcaccgc aatgggaatc gactttcatc ttaactgtga aattggccgc gatattacct
    20821 ttagcgattt aacttctgaa tatgatgcag ttttcatcgg cgtggggact tacgggatga
    20881 tgcgagcaga tctgccgcat gaagatgcgc ccggtgtcat tcaggctcta ccgttcctga
    20941 ctgcccatac ccgccagctc atgggattgc cggagtctga agagtatccg ctgacggacg
    21001 tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac gacaatggat tgtttgcgga
    21061 cttccattcg cctcaatgct gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta
    21121 tgccgggctc gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag tttcagttca
    21181 atgttcaacc gcaatatatc gcttgtgacg aagatgggcg tttaactgcg gtgggcctga
    21241 ttcgtaccgc catgggtgag ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg
    21301 gttcagagtt tgaattgccc gccgatgttc tgattatggc ctttggtttc caggcgcatg
    21361 ccatgccgtg gttgcagggc agcggaatta aactcgataa atggggcctg attcaaaccg
    21421 gtgacgtcgg gtatttacct acccagacgc atctgaaaaa agtctttgct ggtggtgatg
    21481 cagttcatgg cgcggatctg gttgtcactg caatggccgc aggaaggcag gcggcgcgcg
    21541 atatgttaac tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc
    21601 ggctgactgt attggctgcc gtacttgtga agtagcctgt gtggtcgctc acccttcaga
    21661 acaggagttg aatgccgatg tctttttacc ccggctgaag gtacagcgac tggacagcat
    21721 cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg cttgccccgt
    21781 gggggcgctg acgatgggcg agcaggtggt gcagaccaat tctgcccgtt gtattggctg
    21841 tcagagttgc gtcagtgcgt gcccgtttgg aatgatcacc attcagtcgt tgccggggga
    21901 tacccgacaa caaattgtga aatgcgatct ctgtgaacag cgggaagagg ggccagcctg
    21961 tgttgaatct tgcccgacac aggcgctgca gttgcttact gaaagagaac tcaggcgagt
    22021 ccgccagcag cgtattgttg ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg
    22081 aaaggcgcgg agaatctatt agattgccca accacccgcg taaaatgcga ccagtgcgac
    22141 aggaacgcta atttgttgcc cgagtaccag actgaatgcg gtaaatgccg tcaaggaaat
    22201 cgcacaacca atcgccattg gcaaattggc ctacaacccc atcagcaacg agccgaagcc
    22261 cgcgaccaga caggtggcaa caaacacagc tgcgggagga aaacctgctt tgcccagcat
    22321 tcccggaacg acgataacgg aataaaccat ggccagaaag gtcgttaagc cagccagtac
    22381 ttcctgacga acggtactgc cacgagcggt aattttaaaa taattatcca gcgcgccctg
    22441 tggctttggt gcgtccggtg tttgtaggat gtctccagac ataatgatgt cctcacgagg
    22501 atgttaaagt tttattatta gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg
    22561 tagatcgaac ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac taaagaaaca
    22621 gagttgtcat agcgcagctg ctgtagcgga gtggcggtgg gttccatcac cacgaaatca
    22681 gcctctttgc caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg
    22741 ccgagcgtgg ccaggtaaaa cgcttcatat gccgagaggc gatagccttg taattgcaac
    22801 actttgtagg cttcgttcag cgtttgcagc atgttgaaag ttgttccggc accgatatcc
    22861 gttcccatgc ccactttgac tttcttctgc ccagatgccg catgcatcgt gaattgcggt
    22921 agcaatcgcc ggagctgcgc catttacacc gatttccgag atcgatttcg ccccgaacgg
    22981 gccgacttta tcgtcgctcg gcaccagcac agcgcggaaa tcacgcggaa tgtcaccaat
    23041 tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg cgtcataaat
    23101 gatctcttcg ctcatactgt ggccgatagc tcgcagggta gcaccgtaga tttgtcccaa
    23161 tgctaattcc gggttgaccg gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg
    23221 gatttcaccc gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat acgggaaggc
    23281 gaaatcaggc gtgatataac tgccagttcc caccagtgaa ccaaagccga tgccggtttc
    23341 gcctttatgg gcaatatccc cgaaactaac ttcgcctttc ttgccgcgca cgacgcccgg
    23401 cgttgccagt tgaacatctg ccactgcctc acctaacatt tgcgcgccgt ggaacagaat
    23461 tttctcccgt agattttccg ctgccaaacg cgccgcgtta cccgagaagc aagtaccgga
    23521 cgaggcatat gcgcctttat caaacaacgc atgatcggta tcaccggaga taacatgcac
    23581 gtcctgcggt gggcagtgca gcacttctgc tgccagtttc gtcactacag tatccagacc
    23641 agtaccaata tccgcaccgc cagaatgaac gataaaggtg ccgtccgatt ccagtttgat
    23701 catgcagtta gcctgatcga tatccgggat acccgatttc tgcatgataa tggcgacgcc
    23761 gcgaccgatg tgccagtcac cattttgcgg tttcggtgac gaccattgga tcatctcgcg
    23821 accctgacgc aggatctctt ccagtgcaca gctggcggcg gaaggaacgg aggtcggcgc
    23881 tttaccttca ccgattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg
    23941 ttcgataatt tccagttggt cgatctgtaa ctgttcagcc agttccgcta atgccatggt
    24001 gatagcgaag ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt
    24061 gctgtagtag gtggtgacct ggaaatcgac gttatcgcac ggatataacg gcagcgacag
    24121 cgccggtccg ttacacggta cggtgagtga gtggttgccg taagggccag tgttggcgcg
    24181 gaaatccatc ttcactgccg tcaggcgacc atcttttttc gctcccagtt tgacggtgac
    24241 tttcgcgacg tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaatag
    24301 taccggacgc ccggtcacgc aggttgccca ggcgcacact tcttccagca ggatgtcctg
    24361 tttggaacca aaaccgccgc caactcgctc tttaatgaca tgaactttat gctgtttcat
    24421 gcccacgagg cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat
    24481 aaccagacga tcgccgtcca tccgggtaaa gcagatatgt gtttcagtcg ggcactgctg
    24541 tgcctgcgtt gagttatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc
    24601 catatcgcca atatgaccat gaatactggc ggcgatattt ttgcgagggc gagaaccgat
    24661 cgggaagttg atgatcatat gctcgccgcg ctgggctgca tggctgttat cgtcttccag
    24721 agtatctggc gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc
    24781 cgccattgct tcgtcgatcg acattaccgg cttaagcact tcatattcaa cgtcgatgag
    24841 cttcagtgct tcgagcgcaa tttcttcact ttctgcgacg accgcagcaa cacgatcgcc
    24901 gacgtgacgc attttcttgc cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct
    24961 ctgacccccc ggggtatagt agatatcagg gcaattcagg tgagtaataa cgtgaacgac
    25021 gcccggtaag gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg
    25081 gctacgtaac attttaatga cgcaggcgtc agccgttacg cagtcttcaa catagcaggg
    25141 tttcgcctgc accattttcg cggcatcagt tttaggataa tgcttgccaa tgacgtcaag
    25201 gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac
    25261 cgccagttca atgacctgat agtattgttg ccagcctgca tcgcggctga acaaaccaga
    25321 aagcgcgtcg tcgatctctt cgcgggtagg tgcggcgatg cgatcgagaa gatcggtgat
    25381 aatcagagct gcagctggat cgttataacc agactgcacc acgccaacat caaccattgc
    25441 ctgttgaacc agacttaact cgttccattt acccagagat tctgcggtac gaatatctgc
    25501 cttctctaac tgtgcggcaa taagcaagga cgcgttaacg atattaccgt taaagattat
    25561 tgcgtcagaa ccggcaaacc cgaagccatc atcactgttg cgtacagagt gcattcccat
    25621 gttaaacaac agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc
    25681 atttaaagta aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac
    25741 aggtcggcta ccagaactcc cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct
    25801 tcctgcggga aaatggcgtt ggcgacagcc tgttcaagtg cattgccttc cagattttgt
    25861 ttttcgacat cgtgcagacg cagtgcttta ctggcgacgc catccagcgc aattcgcata
    25921 ccgtcgtggt ctgtcattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg
    25981 ctaattttgc gggtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttagcagg
    26041 cgatcgcatg ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc
    26101 agttcagcat ccagtgctag caggacggga agcagcactg actcttcctg ccgggcggcg
    26161 atttcaccac caatggtcga ctgattacga acatggcgtg agtaaacaaa accgagggct
    26221 tcacgcagtg ctgcaggaat aaagcgcgca tcacgcagtg gctgcaagcg agacattgca
    26281 ccaatccgca gtgcaccgtt atcccagtca acccagtcca gttccagatc ctgtaaggaa
    26341 atggcaatct ttttatcggt acgggttggt gtagcattga gtttgctgcc cccggcgaac
    26401 cagacggctt catcctggta gcggcgcttc agttccagcg cctgttcgac agagtcgggc
    26461 ctgaaaaatt gttcaatcat cttctttcct tatttaaccc tcttccctgc gggaagaggg
    26521 catctgtcat ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg
    26581 cttgtgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacac
    26641 cattgaccat cacgctgtga acgctgcctg agcccatacc gaaagcgata tgcccggcga
    26701 tattgtctgc cagcagcggc gtcggcgagt tgtaatcgca aatggtcaaa tcagctttgt
    26761 aaccggcttc cagaagccca aatttcgcgc caaagttgcg gctcatcagt tcgttaccgt
    26821 tagtcagggc tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa
    26881 agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt cccaatgcca
    26941 ggttgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg
    27001 cgttatgcac caggaacgca tcgcgctgat tgagtagggc gatgtcatct ttcgacaagt
    27061 acagcccatg agcgaccagc gttttgctgt cgatgaggtc gaattgcgcc agtcgtgcca
    27121 gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt
    27181 gaatatgcaa accacggcct gtggctttca cggcttcacg cagcatctcc agaccggcat
    27241 ccggcacggt aaacggcgcg tgagcaccga tatgtgcttc caccagatac ggctcgcttg
    27301 tcgctttctt cgcctcatca atcaaacggg cgaaacggat gttttcttct acaccttcct
    27361 gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc
    27421 caacttttaa aaatgcgtcg cgcaatgtgg agagcgaccc gccgatatac gccggcgagg
    27481 catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagtc
    27541 cgctgtaata gagcgactct tcatcaaggg cgcgatcgag ccgccaccag agatttttca
    27601 gcgttgagat gaaatccggg caaggggcga tgtttgccat aattccgcgg gaaagctccg
    27661 agtaaaaatg gttgtgcgag cagacaattc ccggcatcac aatccgacca tgcatctctt
    27721 tgtagctggc atcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacgtcat
    27781 tttcgatagc gatatcaacg ccttcctgca ctttcgccgg gtgtaactgc actgcggtga
    27841 cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg
    27901 gtggacatgg ctgatgatgc ggcacatatc gttcagctcc ggtggtacgt tgttaaactg
    27961 accttcgctg tcgatgttta acacccagct ttggttattc agacgtactc gtacccggca
    28021 atcttccaca aggaagcctg gggtgctgct gttatcaaag tcttgcgcca ggctgaagac
    28081 ggtgattttg tcttcgtatg gtttaccgtt ccacggacag aactgagcgc agttgccgca
    28141 ttcgttacag taagcgtcga ggtgcagcgt ctggaatcta ttctggaagc cagggactgc
    28201 aatggagacg ttggcgcggt tcgggcagac atccacacac ttgttgcaaa cgtagttaca
    28261 ttcgaggcag cgagcggctt cctgggcgac aaacgcgtca cgatcgtcac tgttcaccag
    28321 cgtgatagag atatcgcctt tacgttgata gatttccgct ggattgacgt tgttccagta
    28381 tttatcgttc tggtgggaac ggatattttc ccgactaagg atggcatcgg tcgcccgacg
    28441 cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa
    28501 gacgtcagtc agacgagttt cgccattatg gtcgacgtct ggccagccgt ttttgtccag
    28561 cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag
    28621 gctgtctaca tgcagtgtta ctgtttcgtt ggtttcaacc ggacggcgac gacctttctc
    28681 atccggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc
    28741 cggattattc aggaaacgga actctacgcc gtcgtgcaac gcttcttcat actcttcgcg
    28801 ccatgcgggc atctcttgta gtgaacgacg gtaaacgatc gttgcttttt ctacgcctgg
    28861 aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta cccgccccga caacgaccac
    28921 atgtttgccc agcttgagcg ctgtaccctt gttgtattca cgcaggaagg ggagtgattt
    28981 ccagacattt tggttgtcgc ccgccagttt cacaccgcta tttttatcag tgccggtggc
    29041 aatcagaaca tagtggaagc cctgattttt taactgttca acggttaaat cgggtgagca
    29101 gccatactca aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct gaattaactc
    29161 tgcaggaata cggaactgag gaatgatatt tttcaccacg ccgccagcat tggcttcgcg
    29221 ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag taacctgctg ccagacccgc
    29281 cggacctgca ccaatcacgg caaccggatg gcgtgaacca gaaccggcgg gtttgtgcca
    29341 gcgttgctta tattcatccc aacctttttc cagcgcgact tttttcagtt cgcggatatt
    29401 cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat cgcaaatatg
    29461 accggtaatg gcaggtaggg cgttgcgttg gtagatgagt tccagcgcgt cggcatagcg
    29521 gtgttcgcca agcagacgga tgtattccgg aatatcttgc ttgatagcgc aggcagtaac
    29581 acagggggca acgtagcaat cggtcagcgg caggtcttct gccacttcaa tacgctcttc
    29641 tggcttccag tgtttctggg tgtattccat ggttaacgcg tctgctgcca gtctgttcag
    29701 tcgttcgacg tcaacatggt caagtcccca ggcgtcggag ccttccagct cgcgcatgca
    29761 ggcacttaag cgcagatagc cgccaggttt cagcaggtcg gttgccatag taatagggcg
    29821 aatacctgta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg aataagaaat
    29881 gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acattgatgg agagcgggaa
    29941 cagcgcacgg cctgacatat acatctcttc accaggcagt gcgcctttat tgttgatggt
    30001 gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg ccagtaccat
    30061 caggcgctcc agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa atgactcttc
    30121 ttttaagcct atgtaaccga aaccgcagac atcgaggatc tcacgaacac gcgcgtaccc
    30181 cagtaaggtc gggttaagtt tgacaaaggt gttgagccct ttttcttcca gcatgtagcg
    30241 gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg tgacgccctg
    30301 caccatactg gtggggatgc gagcgggtaa ggcttgcaag ttttcgcgtt tttcctgcaa
    30361 tccgtggcgg gctaaaaatg cgtcatcctg gagtaattta ttcagcgtat cgcgatattg
    30421 agcgaatttc ggatggtcag atgcgtccat catattgtcg atgaactgtt gcatcggcgg
    30481 ttgcttaata ccttcgaggt tgtaaccgac gctcatatta aagatgaacg atttaccgga
    30541 atcagaaggc tgtaacatcg cttcgagaag gtgcagggca aaccaggctt tgaggtattc
    30601 atcccaggct ttaagcaagg taaattcggt agaccattcg gtgttaaagc attcgtcttc
    30661 ggcatcaata cagggctttt ccagctccag gcggtcaaga atttggacgg tttttagttc
    30721 gatgaagcgt ccgccagtca gccaggaagt gacaatattt tgcgcgagct gcgtgtgcgg
    30781 gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa cagttttacc
    30841 ttttacaggt gagtaaaact gttgctcggg aataccaaag attgagcgtt gttgttggta
    30901 ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa tatcccccat
    30961 aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt
    31021 ttagggggat agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa
    31081 atgtgaggtg gctcgaaact taaaggcttt ttgtgaatga actcacaggt gccaaatgag
    31141 aattaaaatc actcctgaaa attattattg tcagcccatt tatcactttt tctcttttct
    31201 caaaatcagt gattccttat cattatgata aagagtgctg gagagattaa atatttgaac
    31261 gcaatcgttt aactacattt tctaaatttt agtatacctt ttttgcgctg taataaagtc
    31321 gtccggagta tcaatatcta aaattatttc tgcattttct atttcgacgg aataatgatc
    31381 tcccataagg agtgcctgac gcatattggt gacattgggt cgctggattg cctgcatcag
    31441 gcatggtttt gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc
    31501 gccatcattt cgtaacgacc agatttttct aaaaatatcg atggtgaggg ttggcatgtc
    31561 accgtgggtg agaaaacaat gttctgtttg cactgcgggt actgcggcct tgacggatgt
    31621 tagtaaaccc tgcgcataat ctgggttgtg aataatagtg atattgctct ggttcgcata
    31681 acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg
    31741 caacgcattt ttgatacttg tatcaagaat tgttccctgt tcccagggga acatcatttt
    31801 ccattgcccc attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat
    31861 agtggttcca ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga
    31921 ttaagttggg gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac
    31981 ccatcggcat tagtcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt
    32041 ggtgctgggg ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc
    32101 aggcgtgtgt taatcacgac gacgacacat atgtttatgc caacgtctca ctggcccgtg
    32161 gttttctgtc gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt
    32221 tgttttcaca gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt
    32281 gatgcactgg tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt
    32341 ggaatgccgt taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc
    32401 gtgattgctg tgatgggagg acatactttg ggcgcgaaag tgagcacaga aaatgtccat
    32461 cgctggtcgc agtttgctga tattaccggg ttaacacctg atgcaacctt gcaactgagc
    32521 gatctcgttg cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt
    32581 cggcgagtct ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag
    32641 ctccttcaac cgctgcaaca acacgacgta gaggcaatct ggctgggcga tatacaagaa
    32701 catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa
    32761 ggatatccat tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa
    32821 attgtccgtt tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg
    32881 cacaaatgtt agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg
    32941 agtggaaggt gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc
    33001 atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt
    33061 cagtatttat cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg
    33121 tcaaccgggc gatagcccag agtgcggcgc tattaggatt tgatattgcc gttgccgata
    33181 tttatcgcga aagcctcaat cctgaactat tcccaccatc aaccacgctt ctccatgctg
    33241 agtcgtttgg tgcggcagtg gaagaactgg atattcgccc tgataatttt gtcctgattg
    33301 ccacgaataa tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt
    33361 tggggttgct ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag
    33421 gcgtggctga agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg
    33481 aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat attacgggtg aaaaataacg
    33541 cgccgggtgg gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc
    33601 gcggtgcggg agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag
    33661 tgatcatgct ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg
    33721 ccgtgttcga tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagctctg
    33781 cggaagcgat gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt
    33841 cattgcttga tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt
    33901 tgggaacgcg ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag
    33961 ggaaggattg tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt
    34021 actctggttg tgcgcaggag aataccgatg ttcctggcaa tattatgggg cataccaccc
    34081 gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc
    34141 tggtgaaaga gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga
    34201 cggggatggt gcgtggcttg ttgaacgacg gcctggcagt ggttggtggt tttaaaattg
    34261 gtgatatcga tcctcgtggt gaaaccgctg atttcaccag cgtttctgat aaagcccggg
    34321 cgattggcgg cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga
    34381 caaaagaagt gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc
    34441 ttaaaattac tgacaaatca ccgttccggt tttgccttca atcccttctt tcgctttgct
    34501 caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc
    34561 cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc
    34621 acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt
    34681 ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct
    34741 ggcccagtct ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac
    34801 agggatacca ccaccgccaa cggcgatcac cacctggcct gcatctacca gagctttaac
    34861 cgtttctttt tcaatgatat caaccggttt tggcgaggca acaacacggc gatagccgcg
    34921 accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt
    34981 aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc
    35041 gacctgagta accagcgttg ctacaggttt attaatgccc cgagaaagca gttcttctct
    35101 taacgcgttt tgtagatcgt aaccaatata accctggctt aaggcaacac agacagacat
    35161 cggcagcatt ggcgagtgtg cttcagtttt tgcggcagct tcaaaggctt ggttaatcat
    35221 gcccacctgc ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc
    35281 aacaattgcc tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag
    35341 cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt
    35401 gattgtgtat agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc
    35461 aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca
    35521 cgggataagg ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg
    35581 gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc
    35641 acgtcgccat cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg
    35701 gcgggcattg cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg
    35761 ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga
    35821 ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc
    35881 gccaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg
    35941 ttacgcagcg gtgggctaag aatgaacttc atgccatctt ctgtatcgta actgcgttcg
    36001 tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca
    36061 agacgcagat aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta
    36121 ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg
    36181 cgactcaagg catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg
    36241 ctggcgatag ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaag
    36301 cggcgtaatg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt
    36361 ttaaaactgc tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta
    36421 atgtgctgga tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca
    36481 cgataaacct ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca
    36541 ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa
    36601 ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa
    36661 acgtaacagc cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc
    36721 aactggcgaa caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta
    36781 acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac
    36841 cggaggggaa taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc
    36901 gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc
    36961 ttcaggttct ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga
    37021 tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc
    37081 gtaggcattc accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa
    37141 gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga
    37201 aacaaccgcg ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc
    37261 ccaggtttcg ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc
    37321 gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa
    37381 ttcgtcataa cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt
    37441 gaaaatggcg ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc
    37501 aatacgaatt tccatacgac cgcgctgacc acggtatatc tggcagtcgg ttggttcggt
    37561 actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc
    37621 gtcgcagtct tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc
    37681 gaggtcttta atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga
    37741 agtaccgcga ccaccgatta gctcatcagt ttccatgcct tcgtacggat cgaagtccca
    37801 gtttttgatg ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg
    37861 gccgtggccg atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc
    37921 gactttttcc atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact
    37981 gggaatagca accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt
    38041 ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc
    38101 cccgttttgc agggagtagt tccaataggg tgattaaggt gctacagcgt gtttgccttc
    38161 ccagacaact tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac
    38221 tacggcatct ttgtccagcg ccagtttttc catcaggctt tgatgttgcg ggtgataatg
    38281 aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc
    38341 gttgccgtac ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga
    38401 gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca
    38461 ggccaggccc gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac
    38521 gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg
    38581 cgggctgtag acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc
    38641 ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc
    38701 tgccagggtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc
    38761 cgtgtcctgt accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt
    38821 atcgtcatag ttcatatccg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac
    38881 gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgtgc
    38941 tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc
    39001 gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc
    39061 acacaataat tgagcgatgg cgtacgcacc gccaagcatt ttgaaggcgt tcagaccgaa
    39121 tcgttttgat tcgtctttga cgagaatttt cttcacacca aaaaggtttg cgagatcgtc
    39181 cagcgcacaa agcggtgttg ggcgataacc agctattttc tgggggaact ggcgcgccag
    39241 tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc
    39301 gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag
    39361 ggccgaccag cagatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag
    39421 ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat
    39481 ataaggcttc cagctggctt ctttgtaggt agcgatacgg tatttttcga atacgccttc
    39541 agtcacttca ccttctttgc aggatacgcc gctgatatca gctggcaggc agtgcatgta
    39601 cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg
    39661 ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt ggtcgttcgc
    39721 acgcagcaat tcagtacgct cttccatcac tttgtaaggt gcccatgact tcggataaac
    39781 gatgtctgcg tctttgaagg cttcttccat gctggtgacc tgacggaagc taccaccgga
    39841 ggctttagca ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg
    39901 atgggccaag gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac
    39961 agagagcggt ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt
    40021 caggttttcc agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt
    40081 cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg
    40141 cttgtaaccg tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag
    40201 atacatatcg tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc
    40261 acgcacggtt tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag
    40321 gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgggtggagt tgtcgcggaa
    40381 tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc
    40441 ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt
    40501 taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc
    40561 attaacagtt ttcatcatac agtcccggta gtggaatgac aggcaaagtg gtttatttca
    40621 ctgtgacttg ccaaagtgat tggtgttttc atagctatac ctgggactaa caataagtgt
    40681 gccagaatta tccgcgcggc tgcaaaagac taatttgacg gataatgtgt ggggttgttc
    40741 acaaattgat gccagcttct ggttgtttgt aaaatacgaa aacgtttggc tttttttatc
    40801 gcgttattta tttgctgcta agtatcagaa ataagataaa ggggcaggct atctctcttc
    40861 agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca
    40921 taattgcatt agaaaatgat aaaaatctta tgtttttatc aaagccaatt tcgcctgagt
    40981 taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct
    41041 ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac
    41101 aattcagcgt tttgccagaa tgcttgccag cgttttgcag tttgaggttg agatcgttga
    41161 tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt
    41221 gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac
    41281 acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgcgaaaa
    41341 agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat
    41401 tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat tttctgatta
    41461 tgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg ggccaggaga
    41521 taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg tattagttgt
    41581 tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt
    41641 acaaaataat attattggga aacctatccg tttcagacca ttaacatttg agagtaattt
    41701 tactcatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat taatcattgg
    41761 tcaattgcat aacattcagg gccgacaatt atttttaatg gcatttcacc aatcgcatac
    41821 cagtttttct gtagcaaatg cacctgatga accacatatt gaacaattgg ttggcgagtg
    41881 ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc catccagcgt
    41941 tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc gcccgagcaa tccataagtt
    42001 gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc cggagcagct
    42061 tctggaaagc gaactgttcg gttatgttaa aggcgcattt actagtgctt ctgccaacgg
    42121 taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg aaataggtga
    42181 tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcgcgtg aaattctgcc
    42241 gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa ctaatcagaa
    42301 tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt
    42361 tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac tattggtgca
    42421 ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca ttgctcccga
    42481 tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt taagcaattt
    42541 gatggaatat ctggttaacg tggttccttc aggtgaagtt atcgacagca cgctattgcc
    42601 gccaaatctg ctgaataatg gcacaacgga gcaaagtgat gtaacagagg tcagtgaggc
    42661 gcacctgtca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa agcaaatgat
    42721 ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt gctgatgaac tgggcatcgg
    42781 cattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat aacctcagtg
    42841 aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa tccagaactg
    42901 tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt ccgcgacgaa
    42961 tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg gtagccataa
    43021 tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga tttcgcataa gcctgctgaa
    43081 catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctcc tttccttcag
    43141 cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac accgtacagg
    43201 caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt tcgcggagta
    43261 attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg ttaatggtgc
    43321 attcgatggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg cggcgacggc
    43381 ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact ctttactggc
    43441 ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag cttccagcgt
    43501 ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt
    43561 tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca
    43621 atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg
    43681 cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga ggatttcgtc
    43741 atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga cgaaacgaac
    43801 accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg tggcagaatc
    43861 tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac ggatctgcgg
    43921 tccagcaatg gacgtggccg cagcacataa cgccgggaga tgacgttgag ttataggagc
    43981 ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat cttccgccag
    44041 cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat aacggtcatt
    44101 gtgatggtgg agctgaatca gtacgtcagt gccatcggcg agcagtttgg cctgcgggtt
    44161 gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag aagcaaaatc
    44221 aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat aaccgttttg
    44281 gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg ttacgaatag
    44341 cagcggcaac aggaattatt ggtggctcac ccagtgactt atgtccgtat gcggattgcg
    44401 gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg gtcggcattt
    44461 tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg atcatctctt
    44521 caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct tctgccagca
    44581 gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg gtgactttgc
    44641 acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccgat gggttagtgg
    44701 tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg tagaaagcgt
    44761 ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc agcacgatat
    44821 ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg gcgtgagcga
    44881 tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca acatagctct
    44941 ggcgtgaggc aaatgcgccg ggagcgaacg gcgtaacgtc ggtatcttga gttgaaataa
    45001 cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag aagacggtgt
    45061 cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca tcctgattca
    45121 tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag gtatagctaa
    45181 aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat tctgcacggc
    45241 gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac cctgcgctgt
    45301 aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa
    45361 tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa
    45421 atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg ttggtatagc
    45481 aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact ttattcctcc
    45541 ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac agaacatcca
    45601 gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca atggtaaaag
    45661 cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact ttcaccggaa
    45721 tgccgccaag cttgctggtc aggaatgctg ccattggctc ttccagtaca tcctgtttat
    45781 taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag ggaatatcca
    45841 gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa
    45901 ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga caatgttgaa
    45961 taacgggggt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt gtttgttgga
    46021 cattacccgt cgacatcgtg ctttgtttca gtaaattgcc accgttatgg attggtgcag
    46081 cgtcttctgc cagcgccgct tctggcgtgg tgataacggg taattcttgc cactcaatgc
    46141 tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg atggcaacgg
    46201 cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt
    46261 tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg aatatcaggc acatcttccc
    46321 aggtaaaaat cgccagtacg cccggcaaac ttctggcttg ttcatcatta atacttacgg
    46381 cataaccatg tgcgataggg ctacgtacat atttcgcata acacatgccc gccataacat
    46441 aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg cgcatgcatg
    46501 attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt tgaacatttg
    46561 gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac gacgataagc
    46621 gttaggggag gtaggaagtg tgatgacgtt aaaaaaacac agatatgata cgaaacagga
    46681 ctctgaatag gggcagtgat acgtatcaat atgagtggcg ataaaatcaa tgtgataaat
    46741 tgggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaagtt cgatacctct
    46801 acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc tctttctcta
    46861 ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc tatgataagg
    46921 cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata aaaaatctct
    46981 gggtatcgtg atgttgttat cggttggact gcttttggcg ggctgttcgg gtagcaaatc
    47041 atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg atacgctata
    47101 tcgtatttcg cgcaccacgg gaaccagcgt aaaagaactg gcgcgactga acggcatttc
    47161 ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga aaagtagcag
    47221 tagtacacgt aaatcaaccg ccaaatcaac gaccaaaacc gcatcggtta caccgtcatc
    47281 agcggtaccg aaatcatcct ggccgccagt agggcaacgt tgttggttat ggccaacgac
    47341 agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga ttgatatctc
    47401 agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt atgtgggcaa
    47461 ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt acattacggc
    47521 ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg ctgggcaaaa
    47581 aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc agattcgtta
    47641 ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg cagggcagca agccaaaatg
    47701 ctgatggcga attaatcagc agtcagcagc gcggcacttg ctaaggagag cgtaaggttt
    47761 ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacgaga gcttcccaag
    47821 ctctatacga gggttcgatt cccttcgccg cttttgctgt atctgatttt ctatattatc
    47881 ttaattcaat cgcttcagac ttttcgcaat cgccgtaata tttccccatt ttgttgtaaa
    47941 taaaaagcgt ttacgcaatg caatcctttt gtaacaaccc aggtaaaaca ccttattttc
    48001 aagttgttaa ataagtggtt atttacgctt ttaacaaacg attgatggaa tattttatga
    48061 aagggaaatc tgcattaact cttctgctcg ctggtatatt ttcgtgcggc acatgccagg
    48121 cgacaggggc agaagtgacg agtgaatctg tatttaacat tcttaattcc acgggggcag
    48181 caacagataa aagttattta tcgttaaatc cagataagta ccctaattat cgtcttctga
    48241 ttcactcagc caaattaaaa aatgaaataa agagccacta taccaaagac gaaattcaag
    48301 ggttgttaac attaacagaa aacacacgaa agttaacgtt gacggagaaa ccctggggaa
    48361 catttattct tgcttctact ttcgaagatg acaaaacagc ggcagaaacc cactatgatg
    48421 ctgtctggct gagggatagt ttatggggct atatggcatt agtatcagat cagggaaata
    48481 gtgtagccgc aaaaaaggtt ctgctcaccc tgtgggatta tatgtctacg ctcgaccaga
    48541 ttaagcggat gcaggacgtc attagcaacc ccaaacgact ggatggagtt cccgggcaaa
    48601 tgaatgctgt gcatatccga tttgacagca actcccccgt gatggcagat gtgcaggaag
    48661 aaggcaagcc gcagctgtgg aaccataaac aaaatgatgc gttaggtctt tatcttgatc
    48721 tccttataca agcaatcgac actggtacaa ttaacgctga agattggcaa aaaggtgacc
    48781 gattaaaatc ggttgcgctg ctgattgctt atctggataa agccaatttc tatgttatgg
    48841 aagattccgg agcatgggaa gaagatgccc gattaaacac atcttccgtt gcactggtga
    48901 cctctggact ggaacgtttg tcgaacttac tctcgaagaa agactctgtt tttgtctctg
    48961 atttactacg cgaagctaaa gccaatgaac tggatgaacc tctttcaaca acgaggttga
    49021 accacctcat agataaaggt tatgaaagaa taacgttaca gcttgattta gggggagaat
    49081 ccccaggtta tctggaaaaa gataaacatt acagagaagc cgatgcggct ttacttaatg
    49141 ttatttatcc tgcaaatctt gcaaagataa acaccagaag aaaagagcag gttctaaaaa
    49201 tagttaaaaa actagcaggt ccgtatggta taaaaagata cgagaaggat aattatcagt
    49261 cggcaaattt ttggttcaat gatattaaaa ccgataccga tcaaaattca catgcgaaaa
    49321 g
//
LOCUS       DAZRVY010000019        48860 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZRVY010000019 DAZRVY010000000
VERSION     DAZRVY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.019"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            389..1606
                     /gene="alaA"
                     /locus_tag="ACHXOX_001103"
     CDS             389..1606
                     /gene="alaA"
                     /locus_tag="ACHXOX_001103"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR7004638.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1690..2289
                     /gene="yfbR"
                     /locus_tag="ACHXOX_001104"
     CDS             1690..2289
                     /gene="yfbR"
                     /locus_tag="ACHXOX_001104"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR7004639.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2348..4180)
                     /locus_tag="ACHXOX_001105"
     CDS             complement(2348..4180)
                     /locus_tag="ACHXOX_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR7004640.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4267..4915)
                     /gene="hxpA"
                     /locus_tag="ACHXOX_001106"
                     /pseudo
     CDS             complement(4267..4915)
                     /gene="hxpA"
                     /locus_tag="ACHXOX_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4926..5420)
                     /locus_tag="ACHXOX_001107"
     CDS             complement(4926..5420)
                     /locus_tag="ACHXOX_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR7004641.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5503..5958)
                     /gene="yfbV"
                     /locus_tag="ACHXOX_001108"
     CDS             complement(5503..5958)
                     /gene="yfbV"
                     /locus_tag="ACHXOX_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR7004642.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6296..7498
                     /gene="ackA"
                     /locus_tag="ACHXOX_001109"
     CDS             6296..7498
                     /gene="ackA"
                     /locus_tag="ACHXOX_001109"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR7004643.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7573..9717
                     /gene="pta"
                     /locus_tag="ACHXOX_001110"
     CDS             7573..9717
                     /gene="pta"
                     /locus_tag="ACHXOX_001110"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR7004644.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9907..11427
                     /gene="yfcC"
                     /locus_tag="ACHXOX_001111"
     CDS             9907..11427
                     /gene="yfcC"
                     /locus_tag="ACHXOX_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR7004645.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11460..12002)
                     /gene="yfcD"
                     /locus_tag="ACHXOX_001112"
     CDS             complement(11460..12002)
                     /gene="yfcD"
                     /locus_tag="ACHXOX_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR7004646.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12060..12611)
                     /gene="yfcE"
                     /locus_tag="ACHXOX_001113"
     CDS             complement(12060..12611)
                     /gene="yfcE"
                     /locus_tag="ACHXOX_001113"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR7004647.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12667..13311)
                     /gene="yfcF"
                     /locus_tag="ACHXOX_001114"
     CDS             complement(12667..13311)
                     /gene="yfcF"
                     /locus_tag="ACHXOX_001114"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR7004648.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13447..14094
                     /gene="yfcG"
                     /locus_tag="ACHXOX_001115"
     CDS             13447..14094
                     /gene="yfcG"
                     /locus_tag="ACHXOX_001115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR7004649.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14151..14513
                     /gene="folX"
                     /locus_tag="ACHXOX_001116"
     CDS             14151..14513
                     /gene="folX"
                     /locus_tag="ACHXOX_001116"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR7004650.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14534..15427
                     /locus_tag="ACHXOX_001117"
     CDS             14534..15427
                     /locus_tag="ACHXOX_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR7004651.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15475..16365)
                     /gene="rpnB"
                     /locus_tag="ACHXOX_001118"
     CDS             complement(15475..16365)
                     /gene="rpnB"
                     /locus_tag="ACHXOX_001118"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR7004652.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16562..17335)
                     /gene="hisP"
                     /locus_tag="ACHXOX_001119"
     CDS             complement(16562..17335)
                     /gene="hisP"
                     /locus_tag="ACHXOX_001119"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR7004653.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17343..18059)
                     /locus_tag="ACHXOX_001120"
     CDS             complement(17343..18059)
                     /locus_tag="ACHXOX_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7004654.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18056..18742)
                     /gene="hisQ"
                     /locus_tag="ACHXOX_001121"
     CDS             complement(18056..18742)
                     /gene="hisQ"
                     /locus_tag="ACHXOX_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR7004655.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18832..19614)
                     /gene="hisJ"
                     /locus_tag="ACHXOX_001122"
     CDS             complement(18832..19614)
                     /gene="hisJ"
                     /locus_tag="ACHXOX_001122"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR7004656.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19835..20617)
                     /gene="argT"
                     /locus_tag="ACHXOX_001123"
     CDS             complement(19835..20617)
                     /gene="argT"
                     /locus_tag="ACHXOX_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR7004657.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLCKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20883..21452)
                     /gene="ubiX"
                     /locus_tag="ACHXOX_001124"
     CDS             complement(20883..21452)
                     /gene="ubiX"
                     /locus_tag="ACHXOX_001124"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR7004658.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21547..23064)
                     /gene="purF"
                     /locus_tag="ACHXOX_001125"
     CDS             complement(21547..23064)
                     /gene="purF"
                     /locus_tag="ACHXOX_001125"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR7004659.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23101..23589)
                     /gene="cvpA"
                     /locus_tag="ACHXOX_001126"
     CDS             complement(23101..23589)
                     /gene="cvpA"
                     /locus_tag="ACHXOX_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR7004660.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23848..24510)
                     /gene="dedD"
                     /locus_tag="ACHXOX_001127"
     CDS             complement(23848..24510)
                     /gene="dedD"
                     /locus_tag="ACHXOX_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR7004661.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24500..25768)
                     /gene="folC"
                     /locus_tag="ACHXOX_001128"
     CDS             complement(24500..25768)
                     /gene="folC"
                     /locus_tag="ACHXOX_001128"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR7004662.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25838..26752)
                     /gene="accD"
                     /locus_tag="ACHXOX_001129"
     CDS             complement(25838..26752)
                     /gene="accD"
                     /locus_tag="ACHXOX_001129"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR7004663.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26908..27567)
                     /locus_tag="ACHXOX_001130"
     CDS             complement(26908..27567)
                     /locus_tag="ACHXOX_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7004664.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27650..28462)
                     /gene="truA"
                     /locus_tag="ACHXOX_001131"
     CDS             complement(27650..28462)
                     /gene="truA"
                     /locus_tag="ACHXOX_001131"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR7004665.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28462..29475)
                     /locus_tag="ACHXOX_001132"
     CDS             complement(28462..29475)
                     /locus_tag="ACHXOX_001132"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR7004666.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29541..30677)
                     /gene="pdxB"
                     /locus_tag="ACHXOX_001133"
     CDS             complement(29541..30677)
                     /gene="pdxB"
                     /locus_tag="ACHXOX_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR7004667.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30776..31771
                     /gene="flk"
                     /locus_tag="ACHXOX_001134"
     CDS             30776..31771
                     /gene="flk"
                     /locus_tag="ACHXOX_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR7004668.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31768..32946)
                     /locus_tag="ACHXOX_001135"
     CDS             complement(31768..32946)
                     /locus_tag="ACHXOX_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7004669.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33221..34441)
                     /gene="fabB"
                     /locus_tag="ACHXOX_001136"
     CDS             complement(33221..34441)
                     /gene="fabB"
                     /locus_tag="ACHXOX_001136"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR7004670.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34600..36606
                     /gene="mnmC"
                     /locus_tag="ACHXOX_001137"
     CDS             34600..36606
                     /gene="mnmC"
                     /locus_tag="ACHXOX_001137"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR7004671.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36727..37005)
                     /locus_tag="ACHXOX_001138"
     CDS             complement(36727..37005)
                     /locus_tag="ACHXOX_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR7004672.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37039..37587)
                     /gene="epmC"
                     /locus_tag="ACHXOX_001139"
     CDS             complement(37039..37587)
                     /gene="epmC"
                     /locus_tag="ACHXOX_001139"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR7004673.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37587..38396)
                     /locus_tag="ACHXOX_001140"
     CDS             complement(37587..38396)
                     /locus_tag="ACHXOX_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR7004674.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38396..39220)
                     /gene="mepA"
                     /locus_tag="ACHXOX_001141"
     CDS             complement(38396..39220)
                     /gene="mepA"
                     /locus_tag="ACHXOX_001141"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR7004675.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39224..40309)
                     /gene="aroC"
                     /locus_tag="ACHXOX_001142"
     CDS             complement(39224..40309)
                     /gene="aroC"
                     /locus_tag="ACHXOX_001142"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR7004676.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40344..41276)
                     /gene="prmB"
                     /locus_tag="ACHXOX_001143"
     CDS             complement(40344..41276)
                     /gene="prmB"
                     /locus_tag="ACHXOX_001143"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR7004677.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41442..41993
                     /gene="smrB"
                     /locus_tag="ACHXOX_001144"
     CDS             41442..41993
                     /gene="smrB"
                     /locus_tag="ACHXOX_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR7004678.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42115..42987)
                     /locus_tag="ACHXOX_001145"
     CDS             complement(42115..42987)
                     /locus_tag="ACHXOX_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR7004679.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42974..43498)
                     /locus_tag="ACHXOX_001146"
     CDS             complement(42974..43498)
                     /locus_tag="ACHXOX_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7004680.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43495..43965)
                     /locus_tag="ACHXOX_001147"
     CDS             complement(43495..43965)
                     /locus_tag="ACHXOX_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7004681.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43962..44510)
                     /locus_tag="ACHXOX_001148"
     CDS             complement(43962..44510)
                     /locus_tag="ACHXOX_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7004682.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44485..45236)
                     /locus_tag="ACHXOX_001149"
                     /pseudo
     CDS             complement(44485..45236)
                     /locus_tag="ACHXOX_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(45256..47898)
                     /gene="yfcU"
                     /locus_tag="ACHXOX_001150"
                     /pseudo
     CDS             complement(45256..47898)
                     /gene="yfcU"
                     /locus_tag="ACHXOX_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(47980..48543)
                     /locus_tag="ACHXOX_001151"
     CDS             complement(47980..48543)
                     /locus_tag="ACHXOX_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7004683.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        12176 a        13105 c        12019 g        11560 t
ORIGIN      
        1 ttaattccgc ttaacaatag tttcacacct tgcccccatt gcgacacctc ggaaatcatc
       61 acgctgttaa actctccgta atgcggaaat atcatcatcg cgtttcatta gtgaattctt
      121 aaatgaggca ttttcacaca attatcttac agataaaaaa accagacttg caattaagaa
      181 tcagaacaat tcactatata acattgcatg caaagcatat acacctcatt attttgtcat
      241 tattaagtta ttaacagcac aatcgagcct tcccctctgg caaaatctta ttctgcagac
      301 cttcaaaaca ccgtcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg
      361 gcgataacag caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga
      421 gaatgtctgt tatgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga
      481 aggtaacaag gtactgaaac tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc
      541 agatgaaatc ctcgttgacg tgatacgcaa cctgcctacc gctcaagggt attgcgattc
      601 caaagggctt tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg
      661 tgatgttacc gtggaagata tttacatcgg caatggtgta tcggagctta tcgttcaggc
      721 aatgcaggca ttgctgaaca gcggggacga aatgttggtt cctgcaccag attacccact
      781 ctggaccgcg gcggtttcgc tttccagcgg taaagcggtg cattatcttt gcgatgaatc
      841 ctctgactgg ttcccggacc tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg
      901 gatcgttatt atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat
      961 ggagattgtg gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga
     1021 caaaattctc tacgacgatg ctgagcatca ctcaattgcg ccactggcac ctgacctgct
     1081 gaccattacc tttaacggac tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg
     1141 gatggtgttg aacgggccga aaaaacacgc caaaggctac atcgaaggtc tggaaatgct
     1201 ggcttcaatg cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg
     1261 tggttatcag agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa
     1321 ccgcgcgtgg gaactgatca acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc
     1381 gctgtatatg ttcccgaaaa tcgacgccaa acgctttaac attcacgacg atcagaaaat
     1441 ggtgctggat ttcctgttgc aggaaaaagt tctgttggtg caagggacgg cattcaactg
     1501 gccgtggccg gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc
     1561 tttgagcaag ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg
     1621 ccggagattg catccggcag cgttatcccg ccacaatgac ctgatgatgt catcatacgt
     1681 aaggtcacta tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac
     1741 cgctggccgc taatgcgcaa cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta
     1801 gcgatggtcg cccatgcgct ggcagctatc aaaaatcgaa aatttggcgg taatgtcaac
     1861 gccgaacgta tcgctttact ggcgatgtac cacgatgcct cagaagtgct caccggcgat
     1921 ctccctactc cggtgaaata cttcaattcg caaatcgctc aggagtacaa ggctattgaa
     1981 aaaatcgctc agcaaaaact ggtcgatatg gttccggaag agctgcggga tatctttgcg
     2041 ccgttaattg acgagcatgc atatagcgat gaagaaaaat cgctggtgaa acaggcagat
     2101 gcactgtgtg catatctgaa atgtctggaa gaactcgcgg ccggaaataa tgaattcttg
     2161 ctggcaaaaa cgcgactgga agcgacgctt gaagcgcgtc gcagccagga gatggactac
     2221 ttcatggaag tatttgttcc cagcttccat ctttcgctcg atgagattag ccaggattca
     2281 ccgctgtaag cagccggagt ctgcgtcgca tcaggcaata agcgccggat gcgacatcag
     2341 gctcttgtca aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa
     2401 tggtgaacgg tacccccaac ttcacaaagt cactgaagct gtaatttccc ggacctaaaa
     2461 ccagtgtgtt aacaggtgaa gaaaccggtg tcataaaggc ggcggatgct gccatcgcca
     2521 cgaccatcgc gaatggataa ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg
     2581 gagccatcaa caccgccgtc gcggtattag agataaatag cccaataacc gccgacaaga
     2641 caaacaaaca ccccagcatc atatgtggcc cgtaaccacc gccaatgtcc atcagccctt
     2701 tcaccgccag cgcgacacct cccgttttct gtaatgccac agcaaacggc atcatcccaa
     2761 cgatcaaaat aatgctcggc cagtgaatgg atttataggc gctttcagca tctatacagc
     2821 ggaatttccc catcagcagg caggcgatga tagcggcaac aggattagga atttcatctg
     2881 tcagcattaa cgccaccatt agcaccagac agaaaatggc atggggtgcc tggctgtgcg
     2941 cgggcgatgc ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt
     3001 tggccagcat accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca
     3061 gaggctcatc cgccagcgaa ccttccagcg ccacgccatt gcgctttagc cccaccacat
     3121 tcagtccgta gcgggtacga aaaccaattt cgcgcaccga tttaccaatc agttctgact
     3181 caggaattaa tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc
     3241 cgcgcagtac catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag
     3301 ccgcagacat atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt
     3361 tcacgataac gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcgccataac
     3421 gctcacgtaa tttgagatca tccagccgtt gaccaatcat tggcgatccg gggcgaatag
     3481 ccagacgtcg cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcgac
     3541 gcgtccagcc ttcgcgctgc ggggtctggg tatccccttt cagcatgaaa cgcatcacta
     3601 acatatacaa aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac
     3661 tgaagccgtg atagccttca cgcagcaatt cactgttgac taccaggttc ggcggcgtcg
     3721 ccaccagcgt catcatgccg cttatcagcc cggcaaaact caacggcatc atcagacgtg
     3781 acggcgaagt ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcgacaa
     3841 cgccggttga actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca
     3901 ttttgatttc actgttgccc gcaactttga ccagccatgt tcccattacg gtggcaacac
     3961 cggtacggac caaaccatcg ccaataataa acaaggcggc aatcaggaca acgttaggat
     4021 cagaaaagcc ggaaaatact tcggggactg tcagcgttcc gcttaatgca aacgcgacaa
     4081 taacaaacaa agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acaatggcaa
     4141 cggccagtaa tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga
     4201 gggagatgtc ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca
     4261 ggtgtttcac tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag
     4321 actgtggagg accaaatcga cctcattcag gcgcggggta tctgccggag cgttaaccgc
     4381 aatgacatga caacccgccg ccaggccaga aagcacgcca gcggagcatc ttccaccacc
     4441 acacactcct gcggcgcaag ccccagcagc tgcgcgccta acagatacgc atcaggttct
     4501 ggttttccgc gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct
     4561 attttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata
     4621 cctgctttat tcaaatgact gagtaaggcg attgcccccg gaagcgggta ataccttcgg
     4681 tttccgtggc ctcgatgtgc tccagacgcg taaactcggc ggcaatatca gcctcggatt
     4741 tgcccgccat aaaatggcgc agagaggtga tcgcctgttt accgtgaatg aaagccagca
     4801 cctcttccgg cgctaaccca tgacgtctgg cccagttgct ccacgcccgt tctaccgcag
     4861 gcagggaatc caccagcgtt ccatcaagat caaacagaaa acctttgcac cgcacgcggg
     4921 cctcctcagg cattgataat ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg
     4981 caggcatgcc acacattaag catgcgctga tatttttccc acattggcgt ctgggcgtta
     5041 aaaccgtgag ttccggcgtc aaaatgggta tagcgccctt ccacattaac cataaagcgg
     5101 acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt
     5161 tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa catgcaacgc atgatacatc
     5221 tccataatgt cgatgatagt gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc
     5281 aattcacgca tctgtaatcc gtaacctcgc tcaataattg tttgcaggcg acggtaacgt
     5341 tcagcatttg ccggatcgag catagtcatc atcttgtact gattagacaa aataagacgt
     5401 tgcgcgttgg tcatttccat tgttgactcc tgtatcactc tactacggtg aaaaaaaaga
     5461 aggctgagta tgccttcttt tatatgcgta atcaggggtc aattacaaat catcaaggaa
     5521 agttttatcc agttgtttga aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg
     5581 cttgccttca acgggtgcca acacctgtcc agactcctgc aatttaccgc gaacttcata
     5641 aaaccagtta aggattgcag ggggtaatgg cgttacagaa cgcttgccca gccaccacaa
     5701 tccctgcatg ggtaaactta aggcgaacag tgcagtggca actgccggcc caagctgacc
     5761 gcccagggca atctgccagc agagagtaaa tacggcgatc ggcggcataa aacggatcgc
     5821 ataacgcgtc atcttgataa cgcgattttc gacaaagacc ggggcaaggc gtttttccag
     5881 tggccacgtc tttgagtaat gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg
     5941 attatccggc gttgacatgc ttcacctcaa cttcacatat aaagattcaa aaatttgtgc
     6001 aaattcacaa ctcagcggga caacgttcaa aacattttgt cttccatacc cactatcagg
     6061 tatcctttag cagcctgaag gcctaagtag tacatattca ttgagtcgtc aaattcatat
     6121 acattatgcc attggctgaa aattacgcaa aatggcatag actcaagata tttcttccat
     6181 catgcaaaaa aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct
     6241 acgctctatg gctccctgac gtttttttag ccacgtatca attataggta cttccatgtc
     6301 gagtaagtta gtactggttc tgaactgcgg tagttcttca ctgaaatttg ccatcatcga
     6361 tgcagtaaat ggtgaagagt acctttctgg tttagccgaa tgtttccacc tgcctgaagc
     6421 acgtatcaaa tggaaaatgg acggcaataa acaggaagcg gctttaggtg caggcgccgc
     6481 tcacagcgaa gcgctcaact ttatcgttaa tactattctg gcacaaaaac cagaactgtc
     6541 tgcgcagctg actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc
     6601 cgtagtgatc gatgagtctg ttattcaggg tatcaaagat gcagcttctt ttgcaccgct
     6661 gcacaacccg gctcacctga tcggtatcga agaagctctg aaatctttcc cacagctgaa
     6721 agacaaaaac gttgctgtat ttgacaccgc gttccaccag actatgccgg aagagtctta
     6781 cctctacgcc ctgccttaca acctgtacaa agagcacggc atccgtcgtt acggcgcgca
     6841 cggcaccagc cacttctatg taacccagga agcggcaaaa atgctgaaca aaccggtaga
     6901 agaactgaac atcatcacct gccacctggg caacggtggt tccgtttctg ctatccgcaa
     6961 cggtaaatgc gttgacacct ctatgggcct gaccccgctg gaaggtctgg tcatgggtac
     7021 ccgttctggt gatatcgatc cggcgatcat cttccacctg cacgacaccc tgggcatgag
     7081 cgttgacgca atcaacaaac tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt
     7141 gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc
     7201 aatggacgtt tactgccacc gcctggcgaa atacatcggt gcctacactg cgctgatgga
     7261 tggtcgtctg gacgctgttg tattcactgg tggtatcggt gaaaatgccg cgatggttcg
     7321 tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg aacgcaacct
     7381 ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt
     7441 tatcccaacc aacgaagaac tggttatcgc gcaagacgcg agccgcctga ctgcctgatt
     7501 tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa
     7561 agaggataaa ccgtgtcccg tattattatg ctgatcccta ccggaaccag cgtcggtctg
     7621 accagcgtca gccttggcgt gatccgtgca atggaacgca aaggcgttcg tctgagcgtt
     7681 ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc
     7741 gtgcgtgcga actcttccac cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa
     7801 ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag agatcgtcgc aaactaccac
     7861 gctaacacca aagacgctga agtcgttctg gttgaaggtc tggtcccgac acgtaagcac
     7921 cagtttgccc agtctctgaa ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc
     7981 gttatgtctc agggcactga caccccggaa cagctgaaag agcgtatcga actgacccgc
     8041 aacagcttcg gcggtgccaa aaacaccaac atcaccggcg ttatcgttaa caaactgaac
     8101 gcaccggttg atgaacaggg tcgtactcgc ccggatctgt ccgagatttt cgacgactct
     8161 tccaaagcta aagtaaacaa tgttgatccg gcgaagctgc aagaatccag ccctctgccg
     8221 gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat cgatatggct
     8281 cgccacctga atgcgaccat catcaacgaa ggcgacatca atactcgccg cgttaaatcc
     8341 gtcactttct gcgcacgcag cattccgcac atgctggagc acttccgtgc cggttctctg
     8401 ctggtgactt ccgcagaccg tcctgacgtg ctggtggccg cttgcctggc agccatgaac
     8461 ggcgtagaaa tcggtgccct gctgctgact ggcggctacg aaatggacgc gcgcatttct
     8521 aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa caccaacacc
     8581 tggcagacct ctctgagcct gcagagcttc aacctggaag ttccggttga cgatcacgaa
     8641 cgtatcgaga aagttcagga atacgttgct aactacatca acgctgactg gatcgaatct
     8701 ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg
     8761 actgaacttg cgcgcaaagc gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt
     8821 accgttaaag cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt
     8881 aatccggcag agatcaaccg tgttgcagcg tctcagggtg tagaactggg tgcagggatt
     8941 gaaatcgttg atccagaagt ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt
     9001 aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc
     9061 ggtacgctga tgctggaaca ggatgaagtt gatggtctgg tttccggtgc tgttcacact
     9121 accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg
     9181 gtatcttccg tgttcttcat gctgctgccg gaacaggttt acgtttacgg tgactgtgcg
     9241 atcaacccgg atccgaccgc tgaacagctg gcagaaatcg cgattcagtc cgctgattcc
     9301 gctgcggcct tcggtatcga accgcgcgtt gctatgctct cctactccac cggtacttct
     9361 ggtgcaggta gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt
     9421 cctgacctga tgatcgacgg tccgctgcag tacgacgctg cggtaatggc tgacgttgcg
     9481 aaatccaaag cgccgaactc tccggttgca ggtcgcgcta ccgtgtttat cttcccggat
     9541 ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt ctgccgacct gatctccatc
     9601 gggccgatgc tgcagggtat gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt
     9661 gacgatatcg tctacaccat cgcgctgact gcgattcagt ctgcacagca gcagtaacct
     9721 cgtcatcatc cgcagctttg cgctgcggat atctgaaccg gaaataatca ctatttccgg
     9781 ttttttattc tcttaatctg cattaatcct ttctgattat cttgcttaac tgcgctgcat
     9841 caatgaattg cgccatttca ctttacatac ttaccacttt gttttgtgca agggaatatt
     9901 tgcgctatgt ccgcaatcac tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg
     9961 ttggtgatta tcttttttgt tgctatttta accagccttg ccacctgggt agttccggtg
    10021 gggatgtttg acagtcagga agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa
    10081 gtcgtagatc cacactcatt tcgcattctg actaacgaag caggcgaacc tgagtatcac
    10141 cgcgtacagc tgttcacgac gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa
    10201 ggattaacct caggatcgaa atacgggaca gctgttggca tcatcatgtt tatgctggtg
    10261 attggcggcg cgtttggcat tgtgatgcgt acaggaacca ttgataacgg tatcctggcg
    10321 cttattcgcc atacccgcgg gaatgaaatt ctctttattc ctgcgctgtt tattctgttt
    10381 tcacttggcg gcgcggtatt tggtatggga gaagaggccg tcgcctttgc cattatcatc
    10441 gcaccgctaa tggtccggct gggctatgac agtattacca ccgtcctggt gacctatatt
    10501 gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag
    10561 gggattgccg gcgttccggt gctttctggc tccgggttgc gcatcgtggt gtgggttatc
    10621 gccactatga ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct
    10681 cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa
    10741 caacgtccgt ttacctttgg tgactggctg gtattgattg tcctgaccgc cgtaatggtc
    10801 tgggtgattt ggggcgtgat cgttaatgcc tggtttattc cagaaattgc cagccagttc
    10861 ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt tccgccttaa cggcatgacg
    10921 gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg
    10981 ctggtgggtt tcgccaaagg gattttgctg ctggtcggta atggtgaagc gggtgatgcc
    11041 agcgtgttaa ataccatcct caacagcatt gccaatgcca ttagcggtct ggacaacgcg
    11101 gtcgcggcct ggtttatgtt gctcttccag gcggtattta atttcttcgt gacgtccggt
    11161 tctggtcagg cggcgttaac catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt
    11221 aaccgtcagg ttaccgtgct ggcattccag tttggtgatg gcttcagtca catcatttac
    11281 ccaacctcag cttcgttaat ggcaactctc ggtgtgtgca gggtggactt ccgtaactgg
    11341 ctgaaggtgg gcgcgaccct acttggactg ctgtttatta tgtccagcgt cgtagtgatc
    11401 ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc cgcaattgcg gctctttttt
    11461 cattctgccg tttcagtctc tacggcttca tttttggcat tgcgcttcat ccacaacgcc
    11521 agcgctttca gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc
    11581 agccagcaaa cttcactgac ttcatcttcc tgtagggcga agggaccgtg agagacgcag
    11641 ctgaacaacg cgccccagac acggcaattt ttatcttcga aatagaactg cccgtgctcg
    11701 gcaaagggga caccggcaat gcccaactct tcttccgctt cgcgacgcgc ggattccagc
    11761 agttgctcat cggcctgaac cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag
    11821 tcttttgtct cggtacgacg ctggaccaga attttgccca tgccatcatg cacgacgatg
    11881 taagttgcac gatgacgcag acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg
    11941 acttcgttct cttcattgac aatatccacc cattcagtac ttgccaaacg acgctgttcc
    12001 attatcagga aaccttcttt ttctggcgct cttacggcgc attttgaatt gtgggtaaat
    12061 tacggattaa tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat tacactaagt
    12121 acgtcattat ccagcatgcc ataactcgcc ggattaccgc ctttcggaat actcaccgag
    12181 ccggggttga agtggaaaat ctccccccgc tgttctgcca ctggcagatg ggtatgaccg
    12241 tacaccagca catcgttctg gtttaaagca ggtagatttt ccgggccaaa aagatgacca
    12301 tgcgtcaaaa acagacgttg tttttccagt aatacctgtt gccacggcgc ggttatcggg
    12361 aaatgcagca gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc gataacctta
    12421 tgtgccactt cattaagccg ttcagcgact ttggctggcg cgtaaccctc cggtaaagca
    12481 ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca gccactgggc accgctttgg
    12541 gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac
    12601 atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg
    12661 ttgctatcag ccagattgct tcgccgaaag tgcaataaaa cgctggacag acgctcgctg
    12721 ccactggaat gtcgcataat ccaccaggcg ttctggcacc tcatcgccat gtagtaccag
    12781 gcggttaatc attagcgcca gatcagtatc agcaatgcac cattcaccaa ataaattcgg
    12841 ctgacccagt gctaacaaat gttctgccat cgcgaacagt ttctctgcac tggcttttcc
    12901 ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc
    12961 gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc cgtcgtgcac gcgcacgatt
    13021 ttctaaatca agcggataaa tacgttccca ggtcggtggc gcaaatcgat cttccagata
    13081 ctccgcaatg gcagaagatt cactcaactc aaaatcatcg atttgtaata acggcacacg
    13141 gcgtgtctga ccgtaacctt gccacgtcgg cttcaaatgt tcaccgctgt cgaggtcgat
    13201 ggtcttgata tgaaacgaca ggcctttttc ctgcaacgcc acccaggcgg ataacacata
    13261 aggggagaaa aagtgggcat ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc
    13321 ggcaataaag ggggctggct atcaaactat agagcctttt tgtcactgtc acgtgacgaa
    13381 tactcacgta agtcgccgcg ctttctatac ttgtggtgtt cagatgatta aacaaccgga
    13441 gctgcaatga tcgatctcta tttcgccccg acacccaatg gtcacaaaat tacgctgttt
    13501 ctcgaagaag cagagcttga ttatcgcttg attaaggtag acctggggaa aggcggtcag
    13561 tttcgtccgg aatttttgcg catttcgccc aacaacaaaa ttccggcaat tgttgatcat
    13621 tctcctgccg atggcggcga accgctaagc ctctttgagt ctggtgccat tttgttgtat
    13681 ctggctgaga aaacaggact ctttttgagt catgaaacgc gtgagcgcgc cgccacatta
    13741 cagtggttat tctggcaggt aggcggactg gggccgatgc ttgggcaaaa tcatcatttt
    13801 aatcacgcag ccccccaaac cattccttac gctattgaac gttatcaggt tgaaactcag
    13861 cgtctttacc atgtactaaa caagcggctg gaaaactcgc cctggctggg aggcgagaac
    13921 tacagcattg cggatattgc ctgctggccg tgggttaatg cctggactcg ccagcgaatt
    13981 gacctcgcaa tgtatccggc agtcaagaac tggcatgagc ggatccgttc gcgccctgcc
    14041 accgggcagg cactgctaaa agcacaactc ggtgatgagc gttcggatag ttaacagaaa
    14101 caggttctcg tgtattattt catcctaagt aaaacaacgg agaacctgca atggcacaac
    14161 ctgccgctat tattcgtata aagaaccttc gtttacgtac gtttatcgga attaaggaag
    14221 aagaaattaa caaccgtcag gatattgtta tcaatgtgac gatccactac cccgccgata
    14281 aagcgcgcac tagcgaagat atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca
    14341 ttattcagca tgtagagaat aaccgtttct ctttgttgga aaaattaact caggatgtgc
    14401 tcgatatcgc acgtgaacat cactgggtga cgtatgctga agtggagatc gataaactgc
    14461 acgcgctgcg ctacgccgat tcggtatcca tgaccttaag ctggcagcgt taaccgccat
    14521 atcgggaggc tgcatgaaca tagtgatcac cggagggacg ggattaattg gtcgccattt
    14581 gattccgcgt ttgctggagc tgggccatca aatcacggta gtgacgcgta acccacagaa
    14641 agccagttcc gttctcggcc ctcgggtgac gctatggcaa gggctagccg atcaaagcaa
    14701 cctcaacggc gttgatgcgg taatcaacct ggccggagaa ccgattgctg ataaacgctg
    14761 gactcacgag caaaaagagc gtctctgcca aagccgctgg aatatcacgc aaaaactggt
    14821 cgatttgatt aatgccagcg acacgccgcc gtcggtactc atttccggct cggcaacggg
    14881 ctattatggc gacttaggtg aagtggtggt taccgaagag gaaccgccgc ataacgaatt
    14941 tactcataaa ctctgcgccc gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac
    15001 gcgagtgtgc ctgctgcgaa ccggcgtggt actggcaccg gacggcggta ttctcggtaa
    15061 aatgctgcca ccgtttcgtc ttggcctggg cgggccgatt ggttccggtc ggcagtatct
    15121 ggcctggatt catattgatg atatggtcaa cggcattctc tggctgctgg ataacgacct
    15181 gcgcgggcca tttaatatgg tttcgcccta cccggtacgc aatgaacaat ttgcccatgc
    15241 gctcggtcaa gcgctgcatc gcccggccat tttgcgcgtc cccgcaaccg ccattcggct
    15301 gttaatgggc gaatcttcaa tactggtatt aggtggacaa cgcgcgctgc ctaaacggct
    15361 ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt
    15421 tcgctgatgt ggtttacagc aaacatccgc cagttaactc ccggtgttac aggattagtg
    15481 gctttgcgcg ataagatcgt ctggtgaaag tcgggtcacc atcataacta actctctgtc
    15541 taaacctcta tccagcatct cctgagcaat acgcagggct tcttcgtgtt tgccctgcat
    15601 tgcgccttct tcacgtaatc tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc
    15661 gcgttctgtt atctcaccaa taaatgcacg gaaacgctgg gcatcccctg tttgtaatac
    15721 gtaattaaac agggctttta gctgtctgtc attagtgttc cctgtaacta gcagcgaaac
    15781 aatttggtcg actaatccca acagatcgcg ctgacgaata tgtttctgaa ttaactccaa
    15841 cagcgccatt ttgcggtgtt gcataatctc gtcatccggc accacggtaa tatccaccaa
    15901 cggaaacgct gatgaatata ttttgcgggc tatagcaggc tcggcaaatt catcaagcca
    15961 gcagagtgaa taaggataag gacttctgca accatgataa aacagcatcg ggatcaccaa
    16021 tggaagctct ttatagcccg catcaagatg gttttgcatt gccgcaatgg aataacgcat
    16081 catgcgaaaa gccattaatt cttccagctt actttggtgc tctatcacta cataaatata
    16141 acccactccc tcctgcgttt tcacagacca caagaggtcg gaataatatt gccgcaggtc
    16201 ttcatcaata aaactgtttg attctagttt aagcgtcgtt aaatcacaca gtttgcgcag
    16261 cggcgcggga agatgaatat caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga
    16321 tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc
    16381 ctcaaattta agatgacgcg acaataacca cgattaacac ccaatgcagc aggcagtttt
    16441 tatctttgga cagcgaattc agaagtattt ttagcggact gcaactttta catttcagcg
    16501 gaagctgact cgcaaattcc ccatgaaaat actccggcag cgttagccac cggagaggaa
    16561 attatttcag cgatccctta aggaactgtt gcagacgagg gctttgcggg ttgccaaata
    16621 actgctccgg cgcgccctct tcttctattt tcccctgatg gaggaaaatg acgtgagtag
    16681 aaacatggcg agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg
    16741 ccagttgctg cataatacgt aacacttcgc ctaccagttc aggatcgagc gccgaggtag
    16801 gttcatcaaa cagtaaaact tccggttcca tcgccagcgc ccgcgcgata gaaacacgct
    16861 gttgctgacc gccggaaaga tgcaccggat atttcccctg cgcacgttcg tctatcccga
    16921 ctttcgccag atacttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct
    16981 gaatcggcgc ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct
    17041 ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg atttttatcg gcgactttga
    17101 gttgaccgtc tttgtcgcgc accagattga tcgtctggcc attgaccacg atcgaccctt
    17161 cactcggttt ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg
    17221 atccgatgat gcttattaca tctccggcat tcgcttgcag tgagacccct ttcagcactt
    17281 catgttcgcc gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat
    17341 tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag
    17401 aggctgatca ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaggcggta
    17461 aaaggttgat acgtggcggc gttaatatcg cgggctattt tcagcagatc cggcacagtg
    17521 gccgtaaacg ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtatgccggt
    17581 aacgcaatac gcagcgcaga aggcaaaata atgcaacgat acattttaaa agtcgagaag
    17641 ccataggctc tggcggcttc aatttcccca tgcggaaccg aacggattgc cccagcaaaa
    17701 atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca
    17761 ctgcggaaga aagcgttaag gaattcggtt cccttaacaa tctcaagcgt gtacatgccg
    17821 gaatagaaca ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac
    17881 cagattggaa actggatgta tttattactg gagacacgac caatcgccag aaacagcgcc
    17941 aggactccgc ctatcactac cgacaaaata agcagccaca gagtgatcgc cacaccagta
    18001 aagcgataac cgtcggtcca cagcagcggt ttccagtatt catgtaagat ttcgatcaca
    18061 ggtcagccct cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg
    18121 aaacagtggt gaaaaccagg taaatcacgc cacagacgat ggcgaaatag aacggttccc
    18181 aggtactttt gcctgccagt tgggtggctt tgaccacatc ttccaggccg agtaacgaaa
    18241 ccagtgcggt agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac
    18301 gcatcatcgc cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg
    18361 tcgccgcctc tatatgtcct ttcggcactg ccataaaagc accacggaac gtttcggtaa
    18421 aataagcacc gtagataaaa ccgagagtga taataccagc gaccatcgga tcgatatcaa
    18481 tctgcccgac gcccatcgcc tccgtcaccg tgtttagcgc aatctgcaaa ccgtagaaaa
    18541 tcagcagcat caacactaag tccggcacgc cacgaatcag cgtggtgtac ccttcgaaaa
    18601 tcagccccga aagccgattt tgcgagagct taccgccagc gccaattaaa ccgatgatta
    18661 cagcgagcac tacagagctg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa
    18721 cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct
    18781 tgtctccctc cggttacccg aagggagcgg gctttctttc accgatggcg attagccacc
    18841 ataaacatca aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat
    18901 ttcggcaaag gctttgttca gtgcttcgcg cagttcgtta tcttctttac gcaggcccat
    18961 gccggtccct acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc
    19021 tttaccgaca ggttgtttga ggaaaccttc gctggcagcg acctcatcct ggaacgcggc
    19081 atcaatacgt ccggcagtca ggtcagaata aatgttgtcc tgcccctgat acgagacgat
    19141 ttcaatgcct tttggtgccc aatgttcatt accgaacgtc tcctgggtgg tgccctgcaa
    19201 tacgcctacc cgtttgcctt tcagcgactc gactgtcggc tgaatgtcag aatttttcgc
    19261 caccaccaaa cgagaatcgg cagcgtacag tttgtcggtg aaggctattt cttgctgacg
    19321 tttttccgta atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg
    19381 gattaacgca tccagcggat tttcgacaaa cgtacattgc gtattgatgc gtttgcataa
    19441 ttcctttgcc agatcgatat cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa
    19501 tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc ggaatcgcag caaatgccgc
    19561 agttgcgctg gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc
    19621 ctcaactgac agtcttttta tagggaatat ttacaggttt gttacactta tcgtgccata
    19681 aaattgacca tcaaggcaaa atattccgcc ccgttagacg tattattttt cattaaaaat
    19741 gcttaagtat gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg
    19801 atcattccat caggtacagc ttcccagcga cgtatcagtc accgtagaca ttaaagtcga
    19861 aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat
    19921 tgaaggcagc cgtcagttca gcatcatctt tacatagccc tacaccggtg ccatcaccga
    19981 agtatttttt gtcttttact gatgagccag caaaggcgaa agctttacca gcaggttgct
    20041 tgaggaatcc ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag
    20101 ccagatcgga atagaccaaa tcctggttgg cataggccac cacatccacg cctttgctac
    20161 gccaggtctc gttagcgtaa gcttcctggg ttgatccctg cagcacacca acatgtttac
    20221 ctttcagtga atccagcgtt ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat
    20281 ctgcggcgta cagcttgtcg gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa
    20341 gcgacgaaat aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt
    20401 cactggcaac ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga
    20461 tatcaaagcc aacaaaatca cctttagcat ctttcgacga gaacggcgca taggtggtat
    20521 cggttccgat acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac
    20581 cgactaacaa agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg
    20641 cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc
    20701 atcttgacga caacagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt
    20761 cctgaagttg aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga
    20821 aacaaaatgt cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg
    20881 agttatgcgc cctgccagcg ggcaaagaga tcttcaggaa gggttattgc aaactggtca
    20941 agaacacgat taaccgtctg atttatcaca tcatcaaggg attgcgggcg atgataaaac
    21001 gccggaacgg gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga
    21061 tggcctaagt gcaatggtgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc
    21121 accacatctg ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggaaagg
    21181 gttttgattg aacagggtaa aatcaccatc cccagcgtct ggaaagaacc ggaagagatg
    21241 ctggcggaaa tatcgcgcgc atcgtgcgtg acatcagcta atgcctgcac ttcgcgcagg
    21301 gaaaaatccg tttcgaggga taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt
    21361 tcgatatctg tgacatcgcg cagaacctgt aataagcgca cgccataaat cgcgccgctg
    21421 gcaccgctga tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc
    21481 agactttgca gtatttcggc gggagttgca agtcagggtg ccagaccggc accctcagcg
    21541 aaggcatcat ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc
    21601 tttggcgtca tcattacgta acgtatcgag gaaatcaagg tagccctgat caacatcttt
    21661 ggtgacgtag acgccgttga acaccgagca ttcaaactgc tggatatccg gattttcagc
    21721 gcgaacggcg tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat
    21781 ctggcgaatt tcatcaactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc
    21841 aataccataa acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt
    21901 cgctccggct tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgatgga
    21961 gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg cggttggcat tcagtttacg
    22021 gcgcacggac ttacgacgca gctgctggcc cggcatgata aaggtgcggc caacatagcg
    22081 gtttttaacg aagccctggc ggtacggttt gcccagaata cgagcaattt ccagcgcgat
    22141 atcacacgag gtttctggga tcgggatcac cacgtcgata tccagatctt cccattcgcg
    22201 ggcaattttc tcgcccagtt tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt
    22261 gtcgatgaac gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac
    22321 cggattgtca gcacattgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc
    22381 gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga
    22441 agcgaccata tattctgtac ggttctcgtc aatatcacgt tttcccagta ccagcggacg
    22501 aatcccgttt ggatcgcgga aagcaaccat accgtggccg ataatcatcg ccacacaggc
    22561 atatgcgccg cggattaagc ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc
    22621 cagcgggtag tggcggaagt tgtccagctc gctagcgaag atattaagca gaatttccga
    22681 gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg
    22741 agcgttggtc agattgccgt tgtgggcaag cgtaatgcca tacggggagt taacgtaaaa
    22801 cggctgcgct tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc
    22861 catattgccc tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt
    22921 cgctttacgc aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg
    22981 accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc
    23041 ggcgataccg acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac
    23101 ttacgctctg ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat
    23161 aaaactgaac tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt
    23221 aaaggagtcg agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa
    23281 acagacgccc agcacccgat cggtgcctga caaccccgtt ttctccacca actggcctat
    23341 cacgaagttc acgatagcac caacaatcag ggtagcgata aacagtaccg cgatggcaat
    23401 tccatttcga accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta
    23461 atgactggca acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa
    23521 gccgcggatc aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat
    23581 ccagaccata tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac
    23641 agaaaaagaa aacgtttgcg tagggatttc cttcccacgc atcaataaaa atggcgctga
    23701 aaaaatattc aacgccatcg actttttatg cctttgcggc atcgtgcaat gcgtgtcgga
    23761 tgcggcgtaa acgccttatc cgacctacgg ttctaacact gcccaggcct gataagacgc
    23821 gccagcgtcg catcaggcaa gactgtatta attcggcgta tagcccatta ccacgccact
    23881 taagccagaa agttgcttca actcacccag cgaacctttc agcttatctt tcgaggcatc
    23941 cggcccaacc agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac
    24001 ccgataaccg gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag
    24061 cgcacccagt tgcacaacat aggctttacc cgttggcgct gctttttctt ccacgaccgg
    24121 cttcggttct ggcttcggcg caggtggtgc ttcaaccttc ggtttaggcg gctccaccgg
    24181 tttcggcttc ggtggggcga ccggtgcagg ttccggttca aactcggtgt tattggctgc
    24241 aatagtggcc ggatcgagcg acggtgctgc ggcatcacct gcccgcacct cttccgctgc
    24301 gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct ggcatcatat caggctcatc
    24361 gcgatcgccc gcttttggaa ccagcgggat agccgcaaac tcatcctgat aatgtttttt
    24421 ctgcccgtcc agcagccctg gaagtacaat cacccccagc gccaccagca cgatcgtgcc
    24481 cactaaccga ttctgaaact tacttgccac cgcttctcct cgcgtcaatc acttccatga
    24541 catgtgcgac cgtgtggaaa gaaccacaca ccagcacggt gtcttccgct ttagcgtccg
    24601 ccattgcggc atcccatgcc tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct
    24661 caagcagttg ttctgccgtg gcaccgcgcg gcccttccag tggcgcacaa taccagtcat
    24721 caaccacgct tttcaaccag gccagagttc cggcaatatc tttatcatgt agcataccga
    24781 taaccgccag cacgcgcccg tttttcggta gcgctttcat acgcccggtg agatattccg
    24841 ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg cgactcgctc acaatctgga
    24901 aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca
    24961 gcccgctggc acgcagtgcc gccagcgctg ttgcggcatt cggttgcggg acaagcggca
    25021 acggcagatt ttccagcgtg ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg
    25081 aatagttcca ctcaacgccc cgacgttgta acagtgcacc tttttcctgc gccacatcag
    25141 caatggtaga aggcatttcc ggctcaccga caattgccgg ttttgcgctg cgaaagatgc
    25201 ctgctttctc gcgaccaata ctttcgcgat ctggtcccag ccagtcggta tgatccagcg
    25261 caatgctggt tactacagcc acatcggcat cgacaatatt ggttgcgtcc agacgaccgc
    25321 ccagccctac ttccagaatc actacgtcaa gttgtgcctg tttgaacagc cacaacgccg
    25381 acagcgtacc gtactcgaaa taggtcaggg aaatatcacc gcgtgccgat tcaatctccg
    25441 caaaagaggc ggtgtgggcc gattccggca attcctgccc ctgcacacgt acgcgctcgg
    25501 tataacgcac cagatgcggt gaactgtaga cgcccacttt gtaccctgcc gccatcagaa
    25561 tcgactccag cgtacggcag gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa
    25621 atggcgctgg tttcaggacg ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga
    25681 gatcgatagt tttactgtgc aggttttcca gataagaaag ccacgaagcc agaggcgacg
    25741 cggcttgagg agtgcgtttg ataatcatgg tatccgctga ttcgttacgg tgagaatagc
    25801 aaaagggcag agccagtggc cctgccctta tcagttatca ggcctcaggt tcctgatccg
    25861 gtaccggggg taccactacg ccttcacgcg gcgcttcagg attcggcgct ggcagattca
    25921 tcaacttcgc cagaatgctc gccagtttca ggcgcatttc cggacgatgg acgatcatgt
    25981 cgatggcgcc tttctcgatc aggaattcac tgcgctggaa tcctggcggc agtttttcgc
    26041 gaacggtctg ttcgataaca cgcggaccgg caaagccaat taacgctttc ggttcagcga
    26101 tgttgagatc gcccagcatg gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca
    26161 gcacggagat gtacggcaag ccgcgctcct gcatttttgc cagtgccgca gaggttttcg
    26221 ccatctgcat cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc
    26281 agatcagcgg gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca
    26341 caacagaccc cattgaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca
    26401 ttccatacag agtgcctttc atcaccacca gcgcatcttt ttcgccggtt tctttctgcg
    26461 cagaagccag acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct
    26521 caagctcgct acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac
    26581 gcgctgtcat acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct
    26641 cagcgcggta taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc
    26701 tcgccttgcg ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc
    26761 tttctgtctg aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt
    26821 gcccccaaca gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa
    26881 aaagtggtcg aaccgcggag ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg
    26941 acgaataaat tcgaaaaccc caggcaatat tgaaacaaaa ataatgccaa caatcatcag
    27001 cttaaggttg ctctgaatga aggggagcgt gccgaagaaa taacccgcgt aggtaaaaat
    27061 cagtacccac aacagtgcgc cgatcacgtt ataagcggcg aaatgacggt acgacatgtg
    27121 gcccattccc gcaacaaacg gcgcgaacgt tctgacgatc ggtacaaaac gggcgagaat
    27181 aatcgttttg ccgccatgtc tctcgtaaaa ctgatgagtc ttatcaagat aactgcgacg
    27241 aaagattttc gaatccgggt tactgaacag tttgtcacca aataaccgtc caatggtata
    27301 gtttactgca tcaccgacaa tagcggcaat caacataagc accaccatca tatggacatt
    27361 gagatcgtta gtttccagcg acgccaatgc accagcaaca aacagcagcg aatcaccagg
    27421 caggaatggc gttaccacca gccccgtttc gcagaacaga atcaaaaaca gaatggcata
    27481 aacccagacg ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa
    27541 atcgatgagg aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctttcag
    27601 gctgtaacta cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca
    27661 gaaatagcgg gcccattggc ggttttggaa gatcataccg gtcagggtaa tccaccgcga
    27721 ccagatacag cccttccgct tttgccgttg ctgccgccag cgttctgtcc tttgccgcca
    27781 gcaactctgc tatccatctc tccggctggt tgtgggcacc gacttccatc aggctgccga
    27841 caatattcct gaccatatga tgtacaaagg cattcgcttt gatatctacc accacataag
    27901 gaccgtgacg cgtgacatta atatgcataa cgttgcgcca cggggttcgg gactggcact
    27961 gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa gcattgcgca gcccgatgca
    28021 tccgttcagc gtccagcggt tcgtaaaaat gggttacccc tttactcaat accgccgggc
    28081 gcagccgatg attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat
    28141 gaaaatcatc aggtacagct ttaacccaac gcacggcgat gtcaccaggt aaattcgcat
    28201 ttacgcccaa ggtccacgcc gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccacct
    28261 gcccggtacc gtgtacccct gcgtcagtac gcccggcgca gaagacggtg atgggttcgt
    28321 tcgccacctg ggagagcgcc ttttccagct tctcctgcac actgcggact tcattctgcc
    28381 gttgccagcc gtaatactta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg
    28441 gcagttgttg ctggtcggac attagtacag atactcctgc accagtttct cggcgatttt
    28501 tactgccatc agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg
    28561 ctccggcata ccgtagtcat tacgcacgca gccaacagaa agatgcggcg tacccgaagc
    28621 atcccctacc tgagtcggga attcgttctc ttcagagagc acaatatctt cgccctgagc
    28681 aaacgcatca cgcgcttctt ctgccgccag cggacgcagt gcttcaaagt tgaccatctg
    28741 ggcatgaccg tagaataccg gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc
    28801 ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct
    28861 atccggcaac aacggcagca tattgaacgc cagttgacga ccgaagaaat cttcttcgtc
    28921 aatcggaatg ccgttgagca atttcgcact ctgccccgct aacgcatcga ccgctttttt
    28981 gccctgggcg gaggctgaaa tcaggctggt aacactgata cgcgacaaac cgccctgatc
    29041 gattaacggt ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac
    29101 attccggttg cggtaatcgg tcagtacaaa cgggtttact tccggcacca ccagcggtac
    29161 gtcgggttcg agagcaaaca gtccgctgct gtcgatcacc aggcaacctg agttggtcgc
    29221 ttcttcaacc caggcagcgg tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg
    29281 cgtccagtcg aattcagcgg catcctgcac agtgattgtt ttaccaccaa agcgcagttg
    29341 ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc ccaaccggga actgacgttc
    29401 agccagcgtt tcaagcaggg cttcgcccac agcgccagtt gcgcccagga cggcaatgtt
    29461 ccagccttca gacatggtgg tttactccag aaatagcaaa gctccctgcc aatgttacag
    29521 cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta
    29581 cacagcaatg atgccgcact ggcgtcatca caaattacat acagagatga ccattcacgg
    29641 cgctcaagat agtttttgcg cagtttatcg aactcacccg gtatcccggc gactttacgc
    29701 agcggtgcgt catcgcggcg cacatcatac accaaatgca ccagcctttt cagcgtcggt
    29761 tgatcgagcg ggccatgcag cgtaatgcga ccaaactctg gcgcgggcag taatgtatcc
    29821 agcgcaacgt gctgttcatg cccaataaac ttgctatagg cttcaaacac ttgcgtagta
    29881 ccgcgtgctt taccttccag ggtataacct gcgatatgcg gcgtgccgat atccactttt
    29941 gtcagcaact ccacgttaag ttccggttcg ccttcccaga catccagcac tacgcttaac
    30001 ttctggcctt cattcaggca ggtcagcaac gcagtattat cgacgactgc gccacggcag
    30061 gcgttaatca gaatcgctcc cggcttcagg ctacggatca gtttttcatc cgccagatgc
    30121 agcgttttgt acggcccatc tttgaagagt ggcgtatgga aagtcagaat atccgcatgc
    30181 tgaactaatt catccagcga gcggaaatca ccctcatccc cacggtcagc gcgaggcgga
    30241 tcgcaaagca atgttttaat ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg
    30301 ttaccaacgc ccacgatccc caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca
    30361 agcatcagta gtgaggagaa aacatattcc accaccgcaa tcgcattaca gccaggtgca
    30421 gcggaaaaac caattcccgc ctgcttcaac catgcttcat cgacatggtc ggtccccgca
    30481 gtggcagtgc caacaaattt aatgggtttt cctgccagca aagattcatt cactttcgtg
    30541 accgaacgca ccatcagcgc atccgcgtct gccagttgag cgacggggat tggacgcccg
    30601 ggaaccgcgg tcacctcacc caaacggcta aataagtcgc gggcataagg catattttca
    30661 tcaacaagga ttttcacgtt tgtgttacct gtatgagacg agagttaacc ggacaagtgt
    30721 gccataatct cgcggccagg catacttgcg aagatttcag gtataaggat acgtaatgat
    30781 acaacctatt tccggccctc ctcctgggca accaccaggt cagggagata acctgccgtc
    30841 tggcgcgggc aatcagcctt tatccagtca gcaacgtact tcgctggaaa gcttaatgac
    30901 gaaagtgacc tcactgacgc aacaacaaag agcagaactg tgggcgggta tcaggcacga
    30961 tattggtctg tcgggagatt caccgctgct ttcgcgtcac ttccctgccg ctgagcataa
    31021 tctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct gcccgccagc ttttagcgca
    31081 attaggggag tatttacgtc tggggaataa tcgtcaggcg gtcacggatt atatccgtca
    31141 taactttggt cagacgccgc tgaatcagct ctcaccggag caattaaaaa ccattctcac
    31201 cctgttgcag gaagggaaga tggttattcc gcaaccacag cagcgcgagg cgaccgaccg
    31261 tcctttatta ccggcggagc acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc
    31321 aacgggggaa cccagcaaac agatctggca atcgatgctg gaactttccg gggtgaacga
    31381 tggcgagtta attccagcga aactgtttaa ccatctggtg acctggttgc aggcgcgcca
    31441 gacgttaagc cagcaaaata cgccgacgct ggaatcacta cagatggcgc taaaacaacc
    31501 tttagatgcc agtgaactgg cagcgttatc ggcgtatatc cagcaaaaat atggtctttc
    31561 tgcgcaatca tcgctttctt ctgcccaggc cgaggatatt cttaatcagc tttatcaacg
    31621 gcgggttaaa gggattgatc cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat
    31681 gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat
    31741 tatcctgatg ctggtctggc tggttcgtta gccccgacga aatgacagta tcgtgacaat
    31801 aattcccagc accgcagaga tcgccccggc aagaaatacc gaagagtaac caaacgtggt
    31861 cgccagcatt cccgccagcg gcccggagac gccgagggcg atatcctgaa acgcggcgta
    31921 accgcccagt gcggtgccgc gaacttgtga ggggacgcgt ttaaccacct ccacgcccag
    31981 cgcaggaaag ataagcgaac atccggctcc ggttaacgcc gcgcccgcta atgcgaccca
    32041 cgcacctggg gcttgccaga gcagcaacaa gcccaccgtt tctacaagca gagagacaat
    32101 cgccactttc acgccgccaa aacggtccgg catccagcca aacatgacgc gcatcacgac
    32161 aaatgcgccg ccaaactcgg taagagtaaa gcccgccatc gcccatcctt tgctggcaaa
    32221 gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa
    32281 ccctggtttc cagataaacc cgacaacgct ccacagcgat ggacgttctc ccgccagggc
    32341 cggtactttg cgcactgtgc cgttacaggc ccacgccagt aagggtaata ccattgtggt
    32401 gatcgccagt gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gagcaccaac
    32461 agcgagggca ccgtaaatcg ccattccatt ccatgacatc actttgccag agtgttttgg
    32521 ccctacgatg cctaaccccc aggtcagagc gcctgtcagt aactggcttt caccaaaccc
    32581 aagaatcaaa cgcccgatga ccaacagggc aaatttgaac ggtgcggaga caggcaaaat
    32641 cgccgccagc agcaacgcgc cgccagccag accacaagct aacatcccct gaagcgccga
    32701 acgttttgcg ccatattgat cggccagtcg cccggcatag ccacgcgtca gcaccgtagc
    32761 cagaaactga atcccgacgg caatgccgac catggtattg ccatagccca gttcatgatg
    32821 aacaaacagc gggataaccg gcaacggcag ccctacggtc atgtaggtga gaaaaaccgc
    32881 aaaagcgatg cggaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc
    32941 agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg
    33001 gtttcagggt tacggtgcgt tggcaggatt taacgcgtac gtcttttcag aaggaaatcg
    33061 acaaagcggg aagtttgcct ggaactggtg gcgattgtca atgatgtgaa aaagggaacc
    33121 atcaggttcc cttttgcgtt agtgccggag gccgcattgg cgcgtaacgt cggatgcgac
    33181 gctggcgcgt cttatccgac ctactccggc ctactgcgaa ttaatctttc agcttgcgca
    33241 ttaccagcgt ggcgttggtg ccgccgaagc cgaagctgtt agacataaca gtggtcagtt
    33301 cgcgatcggt cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc agctcttcaa
    33361 tgttgatgct cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct
    33421 gcacacccgc agcgcccagt gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc
    33481 tcttatcgcc gaacacttca cggatagctg ccagctcttt cacgtcgcca accggagtcg
    33541 aagtaccgtg agagttcagg taatcgattg gggtatcaac gccatgcatc gccatctgca
    33601 tacaacgtac tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc
    33661 cgtagccaac gatttcagca tagatgtgag caccacgcgc cagcgcgtgt tccagctctt
    33721 caaccactac cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag
    33781 tacgggaggc tttttccggg gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga
    33841 attcgcaggc catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt
    33901 tgcccagttg gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg
    33961 agctgatgga gtagttaacg ccatgaattt taaacggcgt ggcgaggcag gcagaaacgc
    34021 cggatgccat cgctttggtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc
    34081 gcattgcgtc agcgccgaat acctggaaac gcggggaacc gccgccagaa cctgcaatca
    34141 ggccaacgcg cgggttattc tggtaagctt ccggagaaag gcccgcatct gcgattgcct
    34201 gctccataga aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt
    34261 caatgaggcc agtggtatcc agttttacgt tgccccagac gtggctacgc atgccggaat
    34321 ccttcagctc ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt
    34381 cctgctggtt attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca
    34441 ttcaatacct ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg
    34501 tacgccgaac aagtccgatc agccatttaa tagagaaatt tgcgcagcct tacacacatc
    34561 gctaagatcg agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa
    34621 cctgccaacc tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc
    34681 tatttttcca acgataacgg gctggaagag acgcgttatg tttttctggg aggcaaccaa
    34741 ttagaggtac gctttcctga gcatccacat cctctgtttg tggtagcaga gagcggcttc
    34801 ggcaccggat taaacttcct gacgctatgg caggcatttg atcagtttcg cgaagcgcat
    34861 ccgcaagcgc aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt
    34921 gcggatttag ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt
    34981 caggcgcagt ggccaatgcc cttgcccggt tgccatcgtt tattgctcaa tgaaggccgc
    35041 gtgacgctgg atttatggtt tggcgatatt aacgaactga ccagccaact ggacgattcg
    35101 ctaaatcaaa aagtagatgc ctggtttctg gacggctttg cgccagctaa aaacccggat
    35161 atgtggacgc aaaatctgtt taacgccatg gcaaggttgg cgcgtccagg cggcacgctg
    35221 gcgacattta cgtctgccgg ttttgtccgc cgcggtttgc aggaagccgg attcacgatg
    35281 caaaaacgta agggctttgg gcgcaaacgg gaaatgcttt gtggggtgat ggaacagaca
    35341 ttaccactcc cctgctccac gccgtggttt aatcgtacgg gcagcagcaa acgggaagta
    35401 gcgattatcg gcggtggtat tgccagcgcg ttgttgtcgc tggcgttatt acggcgcggc
    35461 tggcaggtag cgctttattg cgcggatgaa gcccccgcgc tgggtgcttc cggcaatcgc
    35521 cagggggcgc tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct
    35581 aatgcgttta cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac
    35641 tggtgcggcg tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg
    35701 ttgtcaatgg atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa
    35761 attacgggcg ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc
    35821 ccagcagaac tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat
    35881 tatcaatatc agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca
    35941 ggagatcagc aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccgt
    36001 ttcagccaaa cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg
    36061 acaacgccgg aactggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca
    36121 caaaatccgg cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat
    36181 acggcgtaca gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg
    36241 caggcacagt gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt
    36301 tgtgccaccc gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc
    36361 gtggaatatg cgtcgttggc ggagcagaaa gatgaggcgg taagcgcgcc ggtttttgac
    36421 gatctcttta tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc
    36481 gagattctgg cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctgacg
    36541 gcgttaaacc cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg
    36601 gggtaatctg ctctcgcagt gtttgccgga tgcggcgtga acgccttatc cgacctacgt
    36661 gtgacgtgta ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc
    36721 cgcaacttac tgctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc
    36781 ctggtcacgc ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt
    36841 cgtatgcacc gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt
    36901 caggtggcca cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaaccat
    36961 gccgtcgatt aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg
    37021 taaaacgtgg caccggcttc agttgagcgc ttccggccac gggaactgtt ccgccgtaag
    37081 ttccggcgtg tgataataat tttgtagtgc tttaatgaag cgtgccggac gctcgggaat
    37141 gccatttgtc agataatcca tcacctgcgc atgtacccgc cgttgaaaca caacacgatc
    37201 cggttcgaag tcgccttcca gattgtcgca actgacgtta aacggatatc ccgccgccac
    37261 acagaacaac cagtcgagtg cctgcggctt cacttcaaca tcttcaaact ggctttgcgt
    37321 ttgggcatca cgcccatccg ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc
    37381 ttttccggca atacaccagt gcgaaatctc atgaattgcg ctggcataaa agccgtgggc
    37441 aaagacaatt cggttatacg gtacttccgc atcagcagga agatagatcg gttcgtcgtc
    37501 gcctttaatc agacgggtat taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa
    37561 ctgctcatag tggtgtgtac tgttcattag ttcatcccca accagtggag gatctcctgt
    37621 ccgtggctat cgtaaagtaa tttggcactc atcaccgccg agacgataac aatcatcggg
    37681 cggatcagct tttgtccttt gctcaacacc agccgtgaac ccatgcgcgc gccgaggaac
    37741 tgccccacca gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag aataaacagc
    37801 agcagaccgc caatgtttga cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg
    37861 aggttgaatc cgcacagcgt aacgaaggcc agcgcgtaaa acgatccggc agccgggcca
    37921 aagaatccgt cataaaaacc gacgcagcca ccggcaatta acgcgaaggg taaaccgtac
    37981 atccggcgct ggcgatcttc ttcacccagc tttggcatca gcaagaaata gaggccgata
    38041 caaatcacca gaatgggcaa aatctgccgt aagacatcag cctgaacgta ctgtaccaac
    38101 agtgcgccgc tcattgagcc gacaaaggtc atggcaatat tgagtttctg atcgcttaaa
    38161 ctaaccactt tgcggcgaat aaagtagata gtggcggaaa tagagccgcc gcaggcttgc
    38221 agtttattgg ttgccagcgc gttagcggga gacattcccg cagccatcaa tgccggaatg
    38281 gtgagtaacc caccgccacc ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa
    38341 aagagaactc ccagcaacag cggggaaacc ataaacaggc tattaaacgt ttccattaga
    38401 tcacgtgctc atccagtagc gcctggcagg aaggcggcaa cggaggcggt gtcttcttct
    38461 caggctttgt tgttcccggt tttagaggtg caaaccagct ttgcagttct gccccgcaac
    38521 catcgcctga tggcggtaaa ggttgatctt cacactccag actatcggca gggcaacgta
    38581 atcgtacatg catatgcgcg cgatgctgga accagggtcg cactttgcgc aaccagtcgc
    38641 gatcggtgcc cgcatcaagg caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg
    38701 tgacgtcttt gtcctgggca gcgagtttga tcaagctgaa aatttctggc ttccacagcg
    38761 tggagacaac gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct
    38821 gcgcggaggt ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc agtccggtct
    38881 ggtggctggc atgaccgccg ttgaaacgcc caccagcggg catccccata tcgccaatca
    38941 gcaccgtacc catgcccaga ttgctcacct ggctactcag acgctggata aacatcacca
    39001 gatccgggtg accgaaatag cgacgctgat cggtacgcat gacctgataa tgttcggact
    39061 gtatcggcaa cgtgtcagcg ccgacaatac agccattaga aaaactgcct atcgattgtg
    39121 cgctacccgg cacaggttgg gttatttttt gccacggcgt cgctgccagg ctagcgctac
    39181 tggcaagcag agccagcagc gcaatcgcgg ttttgttcat tttttaccag cgtggaatat
    39241 cagtcttcac atcggcattt tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg
    39301 ccagcatcgc ttcagcgatc ggcactgcac ggatcccgac acagggatcg tgacggcctt
    39361 tggtgatcat ctcaacttct tcgccaaagc ggttaatagt gcgccccggc acggtaatgc
    39421 tggaggtcgg tttcagcgcc atatgagcaa tgatttgctg cccgctgctg ataccgccga
    39481 gaatgccgcc cgcatggttg ctctggaaac cgtctttggt gatttcgtcg cggttctggc
    39541 tgccgcgcag cgccactacg tcaaaaccgt cgccaatttc cacgcctttc accgcgttga
    39601 tgctcatcag cgcatgggcg atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc
    39661 cggcggggac accactggca acaacggtga ctttcgcgcc gatggagtcg ccctcttttt
    39721 tcagcgcgcg catcagttca tctaacgcgt cgattttgtc cgggtccggg cagaagaacg
    39781 gattttgttc aaccagcgac cagtctttga tttccagcgg aatgtcgccc atctgggtca
    39841 agcagccgcg gatttcaata ccaaatttct cggcgagata ttttttggca atcgcccctg
    39901 ccgccacgcg catggcggtt tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca
    39961 gaccgtattt ttgttcgtag gtgtaatcag catggcctgg gcggaaaacg tctttaattg
    40021 cgctgtaatc ctgagagcgc tggtcagtgt tttcgatcaa caagccaatg ctggtgccag
    40081 tagtaacgcc ttcaaaaaca ccggagagaa ttttgacctg atccggctcg cggcgctggg
    40141 tggtatagcg cgatgtccca gggcgacgac ggtcgaggtc atgttgcagg tccgcttccg
    40201 tcagcggaat gcctggcgga acaccatcga cgatgcagcc gagcgccagc ccatgcgatt
    40261 cgccgaaggt ggttacgcga aagagttgtc caattgtgtt tccagccatc acggctccgt
    40321 tattgttgtg tttgcgtgtt tacttaatct ttataaatcg cgaaatattc acgggccgcc
    40381 agcagctgct ctttggtgag cataaacaca ccatcgccgc cgttatcaaa ctccagccag
    40441 gtgaacggaa catccggata ttgttccata agatgtacca tgctgttgcc gacttcacaa
    40501 atcaacacgc catcatcagc aaggtaatct gccgcgtttc caagaatgcg acgcgtcagt
    40561 ttaaggccgt cggtgccaga tgccaggccc agttccggct cgtgacggta ttcgtttggc
    40621 aggtcggaca tatcttccgc gtcgacatac ggcgggttag tgacaatcag atcgtactgc
    40681 actttcggca aatcacggaa cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg
    40741 tgttcttcga tgttctgttc ggcaaccgcc agcgcgtcgg gagagatatc taccgcgtcg
    40801 acttccgctt ccgggaaggc ataagcacag gcaatggcga tgcagccgct accggtacac
    40861 atatctaaaa tatgctgcgg ttgcttgcta atgagtccgg caaatttatt gttgatcagt
    40921 tcaccaatcg gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc atgaccgcag
    40981 aaccaggctt tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg
    41041 cgttcaacaa tacggtgttt ttcgctggag gtcagacgcg cggtgcgcat atcttccggg
    41101 atatccagcg gcaggtagag cgatggtaac accagctgta cggcttcatc ccacgggtta
    41161 tcggtgccgt gaccgtacca gatatttgcc gcgctgaagc ggcttaccga ccagcgcaac
    41221 atgtcctgaa tggtttgcag ctcatttact gcttcatcaa cgaaaatttt atccacgtat
    41281 tcctccaggg catgatcgca ataatttcgg cggctagttt gccatgaaga tgacgataaa
    41341 tcagcattca cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga
    41401 gtcgggtaaa ctgtaggaaa attagaaata gagacagata aatgaaaaag aaaacaacac
    41461 ttagcgagga ggaccaggct ctgtttcgcc agttgatggc ggggactcgc aagattaagc
    41521 aggacacgat tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct
    41581 tgatccagga gcaggctgat gccagccatt atttctccga tgagtttcag ccgttattaa
    41641 ataccgaagg tccggtgaaa tatgttcgcc cggatgtcag ccattttgag gcgaagaaac
    41701 tgcgccgcgg cgattattca ccggaattgt ttttggattt acacggtctg acgcagctgc
    41761 aggccaagca ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg
    41821 cctgcgtgat gcatgggcac gggaagcata ttttgaagca acaaacaccg ctgtggctgg
    41881 cacaacatcc gcatgtgatg gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg
    41941 ctttgttggt gttgattgaa gtggaagagt ggttgccgcc ggagttgccc tgaatgctgg
    42001 cagcaaatgc cggagcagcg taaacgcctt atccggccta caaatatgtg ataattcaat
    42061 aaattgcaat tcacttcgta ggcctgataa gacgcatcag agcaatacta cgaactacag
    42121 ttcattcaat acatactgaa aacgacacgt cgaatcaatc ggttgcccac tactggtatt
    42181 cacattcacc acaatatcgt gtagtacttt atccgttacc ggatgacgag ttggcgtaac
    42241 cgttaccgtt gatttatcac aaccgtaaga ggtaaaggtt aacaattctg ataccgcgcg
    42301 gcagtatgct cctctgccat cattatcaga aaaattactg ccatcacaga gtctgccgcc
    42361 ttccatcagg taggtcgaaa gcgtggtggt cactgttgac cccgtggact gaattcgtaa
    42421 cgccttatta tggctgacat aattttcact ccaggagata tcgtatgaag tgaccggtat
    42481 cgttgggcat attgtgttat gcactgtagg ggccgtatag cttccaaatc cgggtaaatc
    42541 tgtataggaa taaaaggatg cgatccaagg gcaaccataa tcatcagatg caacggtaat
    42601 tttaaacgtc atatatgcac aacgaaaaat tttcgtatct atgccagggc aatttgagtt
    42661 attaattgta tcaaagtcag tcagtaccaa agttggagct gaacccgacc cagaccagga
    42721 aataacccgc aacgtatcgc ctttatacgc aaaagaatca tatttcccat atgtaacctc
    42781 ctttggcgtt atcaccgtaa cattgaaaac actgctcgct acagcaccgg ttaccttgtc
    42841 catggcataa taaactctca cactgtcaaa actcgcactc ataagtttat ctccagcata
    42901 actcttagat gcgccgacaa cactcagaac gagcagcatt aggctcgcga aaattatggt
    42961 ccattttttc atttcagaaa tactcaaagg ttgccgtcac tgtggcacta aagtctccgg
    43021 acgttacatc tcgcccgctc tttgcctgta accaggtatt aaaatttagg ctgttatttc
    43081 cctgattcag cgggagcgtg acgcctgttg tattgttgat tggcaccgat gtgcctgccg
    43141 cagtttcaat accaatccca gcgccggatg ctgatgacga ggagtcaaag gccagaaagc
    43201 cgggtagagc actatcttct gttcctgtca acgttacttt gacattaaac aatgtagaac
    43261 tatgacaatc tttcaattga aacaccaatg gcactcgctc tgactgccca aggttcatta
    43321 aatcgctggc ggcaatggca ggaaaatgta cttctgccaa ctggctacca ttaatcactg
    43381 gcgtacacga tttactgatt aaattgccgg taaagtgcaa attatctcct gctattgtgg
    43441 ttccactgcc cagcaacatc aggctactaa taaaaaacaa cgtacgcttt ttcttcattg
    43501 gtaatctacc tgtaacgtgg cccaggcctc aaagtccgcc tctggcagaa cgacaccact
    43561 cttttttact ggcacagccg ttaacaccgg taaatcggtt tcattcacca taagtggcgt
    43621 attgatcgta aaactctgcc ctgcctgttt gagctgaatg cctaaaccag tgatattgct
    43681 gcttactgca gcattatcaa aatcgcttag tgtgccactc cagctcaagg tcatttccat
    43741 taatgggtcg cgaaccgccg tatcgcaggt aatggaatac ggcacggttt gctcataatt
    43801 gctgccatta atgtcatcaa taagcacatc ctgaaaagtt atttcaatgg tttgagcgtt
    43861 gctaatcgtg cagttaggcg gctgcactaa ataaccgtga aaagagacgt ttgagagtgc
    43921 catcgagcaa aagccccaca gcaaaataag cgagatccgt ttcattggta atccaccaca
    43981 attgtggcac tggcgttaaa ttcagctgcc gctaacgttg taccactctg tttcaccggc
    44041 acagcttcca gaacgggaac tcccgaggaa aaattaaatg gcaaccatga gccagaggtt
    44101 aagtcgagta ttgtgtgatc actacttttc tgaacccgaa taccaaaccc aggaataccc
    44161 gtgctgataa ctgtttcacc gttaatcacg accgttgtcg cctgcatctg tagacgcagg
    44221 tcatccattg ccagattgtt acaaacaagc gagtagtcaa tgggacgttt ataatcaacc
    44281 ccattgatct tattaatgaa tacattacca aactcaactg ccgcaccatt taccgtacaa
    44341 ggaggagggg catcgacaag cacccgtaaa ttcatactta ctgacttact cgctgcaact
    44401 gcaaagccgc taccaacaca acatatcagt aacccagtta ccgcccagac agaacaagat
    44461 cctttcatac tggctctccc ggcctcagcc tttgcggctc tgctcttcat cgacctggca
    44521 actgttaccg ttgcaagtga aaaagagcgg catccgagcg ccatagtcgt tgacataggt
    44581 cagtaccggc acgctaccca tcttcacatt cagcggtgca gaggttttcg gcgacatcac
    44641 tattggcgag aaaccggctg caggattgcc atttttctgt gtgctggcat tactgaaaat
    44701 gacgtaatac ggcgtcgggt tattgacggt aaattcctga ccgttgcggg ttaaagtaac
    44761 cttatattgc caggggtttt tcatgctgac gttctccagc gccttcggtc gccagaacaa
    44821 cttaatacgc gtttgtagtg ctatttgcag cgtgttggct ttgtttgatt ttggcgggat
    44881 ctcacgcacg ttgaagtaga acaggctttc acgatcggca ggcaatttgt tgatatccgg
    44941 cattccctgc acttttacct gcccgttcat cattgaatcg atgcgttgca ccggtggcag
    45001 aacagttaac ggtgaactga tcttgttacc cttttcatct tcgatccagc tttgggccag
    45061 ataaggcagt ttcggatcgt tattgcgcaa cgtcacgctg attgatttgt cgctttcatt
    45121 aaatatcaaa cgggtacgat ctggcgttac agaagcctgg gcgacaaact agcagcgcta
    45181 agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc agacatggat
    45241 cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg tgggtgtcac
    45301 ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg gcgcgacgtt
    45361 gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg
    45421 caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt
    45481 caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc
    45541 attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca gcaccgccat
    45601 cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt cagttagcgt
    45661 ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga ggtcgatata
    45721 cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt
    45781 ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg catcaatcaa
    45841 caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt
    45901 tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact
    45961 gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt
    46021 gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc
    46081 gacctgactg ctgtccgtcc cactgccata gttaccgtta tagctaacgg tactgttgtc
    46141 accccacggc atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta
    46201 acggtagcca gtcattgaga tgctgacatt acgaatactg cccatattga aatagtgcga
    46261 gagcatgatg ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt
    46321 gagataaacc gaaacacccg catcgcggaa gttctggtta taagtggcag tgtacatctc
    46381 tttgtcgttg cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt
    46441 cataaagttc tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc
    46501 aaagtctttg gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc
    46561 ggtgtcttta tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt
    46621 agacaaatcg cgaccgacgc caagcgccgc agactgatag ttttcatctc ccagtgcgcc
    46681 gccatataac gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc
    46741 accctcgaca tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg
    46801 acctggacga gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc
    46861 gttctgttct tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat
    46921 acgaaacggt ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt
    46981 cacttttgct gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg
    47041 aggcaacatt tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat
    47101 atcggatctg aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg
    47161 ctaggcatag tagcgactcc actcccattt tttttgagtt tcatcgccgc tgaattcatc
    47221 gtcatcatta ctgcgagtat gttgatagtt agtctgccag tcagcacgca tacgccacgg
    47281 ccccaggtta accccgaccg tcccgttgcc gctgatctcg ttactatcat caccgccatt
    47341 ttcttcgtgc cgggtttgtg cgttgatgct gtagtccgcg acgatcccgg agatgccgtc
    47401 atcccaacgt gaaggcggat cccagtcggg ataagtatat tcgaggtaag cctgtggcag
    47461 tgaaatgact aatgcggact ggcttaaatc agccttaatt tccatgcctt ccagttgacc
    47521 ggatttcagg catttagcat cgtggctcca ttgcagattt ttcgccacgt cttcttttaa
    47581 accaaactgc gctaccagtt ccggtgtcag acaagcatag cttttgctcg cgtcatcttc
    47641 accagcatac cagtaaatat cgtactcttc cgccaatggc tgtttattta gttgaacctg
    47701 taaattgtat tttccgggct caacatatcc ctggctggaa aaacgcttca gatcaatttt
    47761 tgtgtcgcct tttaattcca gaaaacggga atcaaactga atgtcgtctt cagcccagac
    47821 actgctataa ctccctgaca ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa
    47881 aagagaatgg tcaggcatac acgtttctat ccgtttttat tcttgttcgg tcaaaatcca
    47941 tttaaatccc ccatagcagt gtcgctatgg gggtatgcct tacaggtaag taatctggaa
    48001 ggtggtattt gcttcgaaat tacccagatc tggcgcatca gctgcgccga ccagccaggc
    48061 tttaaagttc agtgtctgct tcgctttgcc tttattggta gaagtgtctt tcactatttt
    48121 ctggtcaata cccatgccgc ttttgtaaga ggtacccaga gagtcaccga tcgccaggct
    48181 aacattgtta aatgccgcac aggtatcggt attgaaaatg gtgtaataat tgccgctatt
    48241 tgcagaagaa acggtaccag taaaggtagt ggtcattgtt tcctgagtat caaatacgca
    48301 atcttgcaga cgaatctgga aacttttcgg cgtggtggtg ccgttatttt tcagtttttc
    48361 ggcaccgata tcacccatat caacttccag tttgtgatcg tccggaacga tagaacacac
    48421 tgagtcttca atggtgccgt aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc
    48481 cgtagaggtg aacacgccca tcaccattgc cgcagcaata gctgttttaa caaacttact
    48541 cattatttgt ccttaaacta taaataatta aaaatataaa cattgcaata cattgattca
    48601 gtcaataacc aatgtttata cccagccatt gaatacggca tgcgatccaa aaagaacagg
    48661 tcgtagaaaa ttgtattcgt tggtgtgggg ccgcatagtg atgtttccac acccctgatg
    48721 caatttaatt ttactaaaga gtaaatgtag atttaattaa tatattgaca gaagaaaggt
    48781 tatcttaagg acatccttaa aaaatacgga ataagtattt agaaaaaata gaaagtatat
    48841 caataatgaa tatcaacagc
//
LOCUS       DAZRVY010000020        44687 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZRVY010000020 DAZRVY010000000
VERSION     DAZRVY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.020"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHXOX_001152"
     CDS             complement(<1..716)
                     /locus_tag="ACHXOX_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7004684.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXOX_001153"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXOX_001153"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR7004685.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXOX_001154"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXOX_001154"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR7004686.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPEINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHXOX_001155"
     CDS             complement(3650..4705)
                     /locus_tag="ACHXOX_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7004687.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHXOX_001156"
     CDS             complement(4715..5503)
                     /locus_tag="ACHXOX_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR7004688.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHXOX_001157"
     CDS             complement(5521..6573)
                     /locus_tag="ACHXOX_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR7004689.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHXOX_001158"
     CDS             complement(6604..7446)
                     /locus_tag="ACHXOX_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR7004690.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHXOX_001159"
     CDS             complement(7433..8314)
                     /locus_tag="ACHXOX_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR7004691.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHXOX_001160"
     CDS             complement(8335..9645)
                     /locus_tag="ACHXOX_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7004692.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHXOX_001161"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHXOX_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHXOX_001162"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHXOX_001162"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR7004693.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHXOX_001163"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHXOX_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR7004694.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHXOX_001164"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHXOX_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR7004695.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHXOX_001165"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHXOX_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR7004696.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHXOX_001166"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHXOX_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR7004697.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHXOX_001167"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHXOX_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR7004698.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHXOX_001168"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHXOX_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHXOX_001169"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHXOX_001169"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR7004699.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHXOX_001170"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHXOX_001170"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR7004700.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHXOX_001171"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHXOX_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR7004701.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHXOX_001172"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHXOX_001172"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR7004702.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHXOX_001173"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHXOX_001173"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR7004703.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHXOX_001174"
     CDS             21883..22047
                     /locus_tag="ACHXOX_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004704.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHXOX_001175"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHXOX_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR7004705.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLSPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHXOX_001176"
     CDS             23556..23801
                     /locus_tag="ACHXOX_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR7004706.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHXOX_001177"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHXOX_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR7004707.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHXOX_001178"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHXOX_001178"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR7004708.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHXOX_001179"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHXOX_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR7004709.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHXOX_001180"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHXOX_001180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR7004710.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHXOX_001181"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHXOX_001181"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR7004711.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHXOX_001182"
     CDS             29464..29817
                     /locus_tag="ACHXOX_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR7004712.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHXOX_001183"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHXOX_001183"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR7004713.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHXOX_001184"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHXOX_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHXOX_001185"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHXOX_001185"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR7004714.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHXOX_001186"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHXOX_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHXOX_001187"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHXOX_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHXOX_001188"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHXOX_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR7004715.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHXOX_001189"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHXOX_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR7004716.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHXOX_001190"
     CDS             36881..37630
                     /locus_tag="ACHXOX_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR7004717.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHXOX_001191"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHXOX_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR7004718.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHXOX_001192"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHXOX_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR7004719.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHXOX_001193"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHXOX_001193"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR7004720.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHXOX_001194"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHXOX_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR7004721.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHXOX_001195"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHXOX_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR7004722.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHXOX_001196"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHXOX_001196"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR7004723.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHXOX_001197"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHXOX_001197"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR7004724.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..>44687)
                     /gene="acnA"
                     /locus_tag="ACHXOX_001198"
     CDS             complement(42720..>44687)
                     /gene="acnA"
                     /locus_tag="ACHXOX_001198"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR7004725.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAAS
                     NELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10536 a        11831 c        11281 g        11039 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatctc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 ctcgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ctgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtattaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacat
//
LOCUS       DAZRVY010000021        43121 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZRVY010000021 DAZRVY010000000
VERSION     DAZRVY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.021"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXOX_001199"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXOX_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR7004726.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXOX_001200"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXOX_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR7004727.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXOX_001201"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXOX_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR7004728.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXOX_001202"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXOX_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR7004729.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXOX_001203"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXOX_001203"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR7004730.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXOX_001204"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXOX_001204"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR7004731.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHXOX_001205"
     CDS             complement(6574..7338)
                     /locus_tag="ACHXOX_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7004732.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXOX_001206"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXOX_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR7004733.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHXOX_001207"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHXOX_001207"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR7004734.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHXOX_001208"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHXOX_001208"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR7004735.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHXOX_001209"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHXOX_001209"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR7004736.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHXOX_001210"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHXOX_001210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR7004737.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXOX_001211"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXOX_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR7004738.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXOX_001212"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXOX_001212"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR7004739.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXOX_001213"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXOX_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR7004740.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXOX_001214"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXOX_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR7004741.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHXOX_001215"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHXOX_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR7004742.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXOX_001216"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXOX_001216"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR7004743.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXOX_001217"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXOX_001217"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR7004744.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXOX_001218"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXOX_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR7004745.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXOX_001219"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXOX_001219"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR7004746.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXOX_001220"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXOX_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR7004747.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXOX_001221"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXOX_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR7004748.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXOX_001222"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXOX_001222"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR7004749.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHXOX_001223"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHXOX_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXOX_001224"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXOX_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR7004750.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHXOX_001225"
     CDS             complement(31717..33003)
                     /locus_tag="ACHXOX_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7004751.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHXOX_001226"
     CDS             complement(33054..33995)
                     /locus_tag="ACHXOX_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR7004752.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHXOX_001227"
     CDS             complement(34010..34780)
                     /locus_tag="ACHXOX_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR7004753.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="ACHXOX_001228"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="ACHXOX_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR7004754.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="ACHXOX_001229"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="ACHXOX_001229"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR7004755.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39286
                     /gene="caiB"
                     /locus_tag="ACHXOX_001230"
                     /pseudo
     CDS             38069..39286
                     /gene="caiB"
                     /locus_tag="ACHXOX_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            39360..40913
                     /gene="caiC"
                     /locus_tag="ACHXOX_001231"
     CDS             39360..40913
                     /gene="caiC"
                     /locus_tag="ACHXOX_001231"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR7004756.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            41022..41807
                     /gene="caiD"
                     /locus_tag="ACHXOX_001232"
     CDS             41022..41807
                     /gene="caiD"
                     /locus_tag="ACHXOX_001232"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR7004757.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41813..42403
                     /gene="caiE"
                     /locus_tag="ACHXOX_001233"
     CDS             41813..42403
                     /gene="caiE"
                     /locus_tag="ACHXOX_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR7004758.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="ACHXOX_001234"
     CDS             complement(42612..43007)
                     /gene="caiF"
                     /locus_tag="ACHXOX_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR7004759.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        10347 a        11739 c        11338 g         9697 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgca atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gttagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttc acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacgtc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg gtcgcacctg
    39121 caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct ggcgcggaat
    39181 gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca gcgaaaacga
    39241 cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc gtggcgtcag
    39301 tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat agaggtgcaa
    39361 tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg gacgtttacg
    39421 gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg tatagttatc
    39481 ttgagttaaa tcaggagatt aaccgcacga caaacctgtt ttatacgctg gggattcgca
    39541 aaggcgacaa ggttgctcta catctcgaca actgcccgga atttatcttt tgctggttcg
    39601 ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgttg cgcgaggaaa
    39661 gcgcgtggat cctgcaaaat agccaggcgt gcctgctggt gaccagtgcg caattctatc
    39721 ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt tgcctgacag
    39781 atgtggcact tcccgctgat gatggcgtga gttcgtttac tcaactgaaa aatcaacaac
    39841 ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa attctcttca
    39901 cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac ctgcgcttcg
    39961 ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac ctgacggtaa
    40021 tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt tctgccgggg
    40081 ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag gtacagaagt
    40141 accgcgccac cgttaccgaa tgtattccga tgatgatccg taccttgatg gcgcagccgc
    40201 cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc aacttgtcgg
    40261 agcaggaaaa agatgcgttt tgtgaacgct tcggcgttcg cttgctgacg tcttatggga
    40321 tgacggaaac cattgtgggc attatcggcg atcgccctgg cgataaacga cgctggccgt
    40381 cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac aatcgcccgc
    40441 tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa accatcttca
    40501 aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac ggctggctac
    40561 ataccggcga taccggatac tgcgacgaag agggcttttt ttatttcgtc gatcgccgct
    40621 gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa aatatcatcg
    40681 ccacccatcc aaaaattcag gatatcgtgg ttgtgggtat taaagattcg attcgcgatg
    40741 aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa gaggaatttt
    40801 tccgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg gagatcagaa
    40861 aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa taaatgaaac
    40921 agcagggaac taccctgcca actaataatc acgctataaa acaatatgca ttttttgcag
    40981 ggatgttgtc gtccctgaaa aagcaaaaat ggagaaaagg aatgagtgaa tcattacatc
    41041 tgacccgcaa tggatcaatt ctggaaatta cccttgatcg tccaaaagcg aatgctattg
    41101 atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac gatccgcaat
    41161 tacgtgtcgc cattattacc ggtgccggag agaagttctt ttccgcgggc tgggatttaa
    41221 aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtgga tttgcgggat
    41281 taaccgaaat tttcaatctc gacaaaccgg ttatcgcagc tgtgaacggc tatgcctttg
    41341 gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat aacgccagct
    41401 tcgccctgcc ggaagccaag ctgggcatcg ttcctgacag cggcggcgtg ctgcgtctac
    41461 cgaagatcct gccgcctgcc atcgtcaatg aaatggtgat gaccggcaga cgaatgggcg
    41521 cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg gaactgatgg
    41581 ataacgcccg cgaactggcg cagcagctgg ttaacagcgc cccgctggcg attgcggcgc
    41641 tgaaagagat cttccgcacc accagcgaaa tgccggtaga agaagcgtat cgctatattc
    41701 gcagcggcgt gttgaaacac tatccgtcgg ttctgcattc ggaggatgcc attgaagggc
    41761 cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa ccgtgagcta
    41821 ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc atcccagtgc
    41881 cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac tcgcctcact
    41941 gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg atggctgcat
    42001 tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata tcgggcacgg
    42061 agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga acagcgtgat
    42121 tatggatggc gcggtcattg gcgaagagag cattgttgcc gccatgagct ttgtcaaagc
    42181 aggctttcac ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg ctgtacgcag
    42241 tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc aggatcttgt
    42301 cgggcgctgt catgcatcgt tacatgaaac acagccactg aggcaaatgg aggaaaatcg
    42361 cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc ggatgcggcg
    42421 taaacgcctt atccaactct gctggagagc aaccgtaggc cggataagat gcgccagcat
    42481 cgcatccggc gatagctgtc ggatgcggca taaacgcctt atccaacccg gctggagagc
    42541 aaccgtaggc tggataagat gcgccagcat tgcatccagc aaccaggctg cgtggtatca
    42601 acgaaatgac attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
    42661 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
    42721 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
    42781 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aactgcgcag
    42841 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
    42901 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
    42961 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
    43021 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
    43081 attgtggata tatcgatagt atgcaacttc accgtcgata a
//
LOCUS       DAZRVY010000022        42133 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZRVY010000022 DAZRVY010000000
VERSION     DAZRVY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.022"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHXOX_001235"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHXOX_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR7004760.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHXOX_001236"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHXOX_001236"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR7004761.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHXOX_001237"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHXOX_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR7004762.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHXOX_001238"
     CDS             4216..4620
                     /locus_tag="ACHXOX_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR7004763.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHXOX_001239"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHXOX_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR7004764.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHXOX_001240"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHXOX_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR7004765.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHXOX_001241"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHXOX_001241"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR7004766.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHXOX_001242"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHXOX_001242"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR7004767.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="ACHXOX_001243"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="ACHXOX_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHXOX_001244"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHXOX_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR7004768.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="ACHXOX_001245"
     CDS             complement(10471..10767)
                     /locus_tag="ACHXOX_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR7004769.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHXOX_001246"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHXOX_001246"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR7004770.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHXOX_001247"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHXOX_001247"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR7004771.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHXOX_001248"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHXOX_001248"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR7004772.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHXOX_001249"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHXOX_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR7004773.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="ACHXOX_001250"
     CDS             complement(16025..16711)
                     /locus_tag="ACHXOX_001250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR7004774.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHXOX_001251"
     CDS             complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHXOX_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR7004775.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="ACHXOX_001252"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="ACHXOX_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR7004776.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHXOX_001253"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHXOX_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR7004777.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHXOX_001254"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHXOX_001254"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR7004778.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHXOX_001255"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHXOX_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR7004779.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHXOX_001256"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHXOX_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR7004780.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="ACHXOX_001257"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="ACHXOX_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR7004781.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHXOX_001258"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHXOX_001258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR7004782.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHXOX_001259"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHXOX_001259"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR7004783.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHXOX_001260"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHXOX_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR7004784.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHXOX_001261"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHXOX_001261"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR7004785.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="ACHXOX_001262"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="ACHXOX_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR7004786.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHXOX_001263"
                     /pseudo
     CDS             complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHXOX_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHXOX_001264"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHXOX_001264"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR7004787.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHXOX_001265"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHXOX_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR7004788.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHXOX_001266"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHXOX_001266"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR7004789.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="ACHXOX_001267"
     CDS             32810..33454
                     /locus_tag="ACHXOX_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR7004790.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="ACHXOX_001268"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="ACHXOX_001268"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR7004791.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34793
                     /locus_tag="ACHXOX_001269"
                     /pseudo
     CDS             34530..34793
                     /locus_tag="ACHXOX_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHXOX_001270"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHXOX_001270"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR7004792.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHXOX_001271"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHXOX_001271"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR7004793.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHXOX_001272"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHXOX_001272"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR7004794.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHXOX_001273"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHXOX_001273"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR7004795.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="ACHXOX_001274"
     CDS             39514..41064
                     /locus_tag="ACHXOX_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR7004796.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="ACHXOX_001275"
     CDS             41036..41899
                     /locus_tag="ACHXOX_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR7004797.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9945 a        11409 c        10590 g        10189 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgctgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gac
//
LOCUS       DAZRVY010000023        39927 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZRVY010000023 DAZRVY010000000
VERSION     DAZRVY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.023"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(138..813)
                     /locus_tag="ACHXOX_001276"
                     /pseudo
     CDS             complement(138..813)
                     /locus_tag="ACHXOX_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(958..1566)
                     /locus_tag="ACHXOX_001277"
     CDS             complement(958..1566)
                     /locus_tag="ACHXOX_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR7004798.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1750..2067)
                     /gene="metJ"
                     /locus_tag="ACHXOX_001278"
     CDS             complement(1750..2067)
                     /gene="metJ"
                     /locus_tag="ACHXOX_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR7004799.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2344..3504
                     /gene="metB"
                     /locus_tag="ACHXOX_001279"
     CDS             2344..3504
                     /gene="metB"
                     /locus_tag="ACHXOX_001279"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR7004800.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3507..5939
                     /gene="metL"
                     /locus_tag="ACHXOX_001280"
     CDS             3507..5939
                     /gene="metL"
                     /locus_tag="ACHXOX_001280"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR7004801.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6078..6212)
                     /locus_tag="ACHXOX_001281"
     CDS             complement(6078..6212)
                     /locus_tag="ACHXOX_001281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004802.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6288..7178
                     /gene="metF"
                     /locus_tag="ACHXOX_001282"
     CDS             6288..7178
                     /gene="metF"
                     /locus_tag="ACHXOX_001282"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR7004803.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7507..9687
                     /gene="katG"
                     /locus_tag="ACHXOX_001283"
     CDS             7507..9687
                     /gene="katG"
                     /locus_tag="ACHXOX_001283"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR7004804.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9781..10686
                     /gene="yijE"
                     /locus_tag="ACHXOX_001284"
     CDS             9781..10686
                     /gene="yijE"
                     /locus_tag="ACHXOX_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR7004805.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10713..11330)
                     /locus_tag="ACHXOX_001285"
     CDS             complement(10713..11330)
                     /locus_tag="ACHXOX_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR7004806.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11604..12707)
                     /gene="gldA"
                     /locus_tag="ACHXOX_001286"
     CDS             complement(11604..12707)
                     /gene="gldA"
                     /locus_tag="ACHXOX_001286"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR7004807.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12718..13380)
                     /gene="fsa"
                     /locus_tag="ACHXOX_001287"
     CDS             complement(12718..13380)
                     /gene="fsa"
                     /locus_tag="ACHXOX_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR7004808.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13392..15893)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_001288"
     CDS             complement(13392..15893)
                     /gene="ptsP"
                     /locus_tag="ACHXOX_001288"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR7004809.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16202..17281
                     /locus_tag="ACHXOX_001289"
     CDS             16202..17281
                     /locus_tag="ACHXOX_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR7004810.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17296..17616
                     /locus_tag="ACHXOX_001290"
                     /pseudo
     CDS             17296..17616
                     /locus_tag="ACHXOX_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17667..19963
                     /locus_tag="ACHXOX_001291"
                     /pseudo
     CDS             17667..19963
                     /locus_tag="ACHXOX_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19929..20807
                     /locus_tag="ACHXOX_001292"
     CDS             19929..20807
                     /locus_tag="ACHXOX_001292"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR7004811.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20809..21150
                     /locus_tag="ACHXOX_001293"
     CDS             20809..21150
                     /locus_tag="ACHXOX_001293"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR7004812.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21137..21988)
                     /locus_tag="ACHXOX_001294"
     CDS             complement(21137..21988)
                     /locus_tag="ACHXOX_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7004813.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22203..23936)
                     /locus_tag="ACHXOX_001295"
     CDS             complement(22203..23936)
                     /locus_tag="ACHXOX_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR7004814.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24119..26770)
                     /gene="ppc"
                     /locus_tag="ACHXOX_001296"
     CDS             complement(24119..26770)
                     /gene="ppc"
                     /locus_tag="ACHXOX_001296"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR7004815.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27122..28273)
                     /gene="argE"
                     /locus_tag="ACHXOX_001297"
     CDS             complement(27122..28273)
                     /gene="argE"
                     /locus_tag="ACHXOX_001297"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR7004816.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28427..29431
                     /gene="argC"
                     /locus_tag="ACHXOX_001298"
     CDS             28427..29431
                     /gene="argC"
                     /locus_tag="ACHXOX_001298"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR7004817.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29439..30215
                     /gene="argB"
                     /locus_tag="ACHXOX_001299"
     CDS             29439..30215
                     /gene="argB"
                     /locus_tag="ACHXOX_001299"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR7004818.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30276..31649
                     /gene="argH"
                     /locus_tag="ACHXOX_001300"
     CDS             30276..31649
                     /gene="argH"
                     /locus_tag="ACHXOX_001300"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR7004819.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31916..32833
                     /gene="oxyR"
                     /locus_tag="ACHXOX_001301"
     CDS             31916..32833
                     /gene="oxyR"
                     /locus_tag="ACHXOX_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR7004820.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32816..34216)
                     /gene="sthA"
                     /locus_tag="ACHXOX_001302"
     CDS             complement(32816..34216)
                     /gene="sthA"
                     /locus_tag="ACHXOX_001302"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR7004821.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34537..35187
                     /gene="fabR"
                     /locus_tag="ACHXOX_001303"
     CDS             34537..35187
                     /gene="fabR"
                     /locus_tag="ACHXOX_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR7004822.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35187..35546
                     /locus_tag="ACHXOX_001304"
     CDS             35187..35546
                     /locus_tag="ACHXOX_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR7004823.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35586..36686)
                     /gene="trmA"
                     /locus_tag="ACHXOX_001305"
     CDS             complement(35586..36686)
                     /gene="trmA"
                     /locus_tag="ACHXOX_001305"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR7004824.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36800..36990
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37055..38899
                     /gene="btuB"
                     /locus_tag="ACHXOX_001306"
     CDS             37055..38899
                     /gene="btuB"
                     /locus_tag="ACHXOX_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR7004825.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38844..39701
                     /gene="murI"
                     /locus_tag="ACHXOX_001307"
     CDS             38844..39701
                     /gene="murI"
                     /locus_tag="ACHXOX_001307"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR7004826.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9115 a        10036 c        11016 g         9760 t
ORIGIN      
        1 gatgtaaaat ataatatatg tcatttgaga aaaaagagca gaatgaggta aaaatctttg
       61 gctgtgtatt cagtctggta atcatccaag gaaagcttat cagaagggtg atgactccaa
      121 ttagtagaac atatattttc gataaaagca ccgatgactt tttcatgaag ttctacagaa
      181 cgcgagaaac agatggttct gcgggccaga cgtttgattc gggttctcag ggtcagattg
      241 ttacgctcaa tgtgctgagt aaaaatctta ccagtcagat gcttctcctt cggtaactct
      301 ctagcgtaac taccccattc gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg
      361 cccgcaattc acgacaggtt tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac
      421 cagttttcgt gttgtacacg taccagagcc agtgctgtcg cgctttactg ccgacaaagc
      481 tccattgctg actaagtttg cagatgagtg ccacatcagc atgagcgacc ggggatgacg
      541 ttattcttcg tggcgagagt tttttaaagt tcggatgact gtattaatgc cgattttcag
      601 cgtacgggca gtatcgcgaa ccccggcacc gttgaaagtc atttcagcga tttggtcttt
      661 aactcccggt ttacggggct cgtaacagta actgagctga aatacccggt gacagtcacg
      721 gccgcggaat cgctcatgtc tttttgggtt ctgcccgtga cggtaaacct ggacggactg
      781 gcaacggggg caatgaaggt taactgtgac catgaaacaa ccttaaaagc gagtattata
      841 tccttaattc aactaattgg aggcatcacc gtttaccgga gcgacagttt gcggaaaggt
      901 cgtgcaacta caaagcaaca aacagactac ggcgggaatt atttttttca tcatccatta
      961 ccacgaaaac agcggccact ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc
     1021 ttgtggtgaa actaccgttt cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt
     1081 agcctggacc agcgggttgc tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg
     1141 cattcccagc gcgttctggt aaaccttcca caccacttcc gaacagtact ggcgatcgtc
     1201 gctccatgag aagctgaaat catacggttt accgagataa cgttttgccg tttgcgccag
     1261 tttttgctgt tgttcaacac tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt
     1321 ttcaccatgg gcgatccact gcttgagcgg ggtgtatttc accgggccga ccgcttcaaa
     1381 aacgtagggc tttttgttgc gtatcaccag cataccggtg tggctataat cggaatgggt
     1441 cgccagttgg atcgctttac tttgcgatga gcgagagatc tgaaagatga tgtcgccggt
     1501 ttgtggctgc caggcaaagg caggtacaga aaccagcagg ctgaggatca gcagcctgtt
     1561 tttcatagtt aaacgtccat gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt
     1621 tatgccggat gcggcgtgaa cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat
     1681 tgcaatatga cgtaggcctg ataagcgtag cgcatcaggc gattccactc cgcgccgctc
     1741 ttttttgctt tagtattccc acgtctccgg gttaatcccc atctcacgca tgatctcttt
     1801 tgccgcttcc gggatttcgt cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg
     1861 ttgcccggta aaggcatgca gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg
     1921 caggttgttc acctgacgac gcgtgcgttc atcggtgagg atttttaaca ccttaagagg
     1981 aatggaaacc gtaatctttt tgacttgttc actcttcttg ccgtgctcag cgtatgggct
     2041 gatatattcg ccgctccatt cagccatgag atacttaatc ctcttcgtca ataaattgag
     2101 accagaccac acaatttatg tgggtactga ccgtaaaccc gcatagttta ccgtacaggc
     2161 gttaccgtga catcgtgtaa tgcacctgtc ggcgtgataa tgcatataat tttaacggct
     2221 atttgggatt tgctcaatct atacgcaaag aagtttagat gtccagatgt attgacgtcc
     2281 attaacacaa tgtttactct ggtgcctgac atttcaccga caaagcccag gaaacttcat
     2341 cacatgacgc gtaaacaggc caccatcgca gtgcgtagcg ggttaaatga cgacgaacag
     2401 tatggttgcg ttgtccctcc catccatctt tccagcacct ataactttac cggatttaat
     2461 gaaccgcgcg cgcatgatta ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt
     2521 gcgctggcag aactggaagg cggtgctggc gcagtactta ctaataccgg catgtccgcg
     2581 attcacctgg taacgaccgt ctttttgaaa cctggcgatc tgctggttgc gccgcacgac
     2641 tgctacggcg gtagctatcg cctgttcgac agtctggcga aacgcggttg ctatcgcgtg
     2701 ttgtttgttg atcaaggcga tgaacaggca ttacgggcag cgctggcaga aaaacccaaa
     2761 ctggtactgg tagaaagccc aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc
     2821 tgccatctgg caagggaagt cggggcggtg agcgtggtgg ataacacctt cttaagcccg
     2881 gcattacaaa atccgctggc attaggtgcc gatctggtgt tgcattcatg cacgaaatat
     2941 ctgaacggtc actctgacgt ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc
     3001 actgaacttg cctggtgggc aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat
     3061 ctgctgctac gcgggttgcg aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg
     3121 caggcgattg tgaaatatct gcaaacccag ccgttggtga aaaaactgta tcacccgtcg
     3181 ctgccggaaa atcaggggca tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg
     3241 ttgagttttg aactggatgg cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg
     3301 ttgtttacgc tggcggaatc attaggggga gtggaaagtt taatctctca cgccgcaacc
     3361 atgacacatg caggcatggc accagaagcg cgtgctgccg tcgggatctc cgagacgctg
     3421 ctgcgtatct ccaccggtat tgaagatggc gaagatttaa ttgccgacct ggaaaatggc
     3481 ttccgggctg caaacaaggg gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc
     3541 gtcagctgca taaatttggt ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg
     3601 cgggcattat ggcggagtac tctcagcctg acgatatgat ggtggtttcc gccgccggta
     3661 gcaccactaa ccagttgatt aactggttga aactaagcca gaccgatcgt ctctctgcgc
     3721 atcaggttca acaaacgctg cgtcgctatc agtgcgatct gattagcggt ctgctacccg
     3781 ctgaagaagc cgatagcctc attagcgctt ttgtcagcga tctggaacgc ctggcggcgc
     3841 tgctcgacag cggtattaac gacgcagtgt atgcggaagt ggtgggccac ggggaagtat
     3901 ggtcggcacg cctgatgtct gcggtactta atcaacaagg gctgccagcg gcctggcttg
     3961 atgcccgcga gtttttacgc gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt
     4021 cttacccgtt gctgcaacag ctactggtgc aacatccggg caaacgtctg gtggtgaccg
     4081 ggtttatcag ccgtaacaac gccggtgaaa cggtgctgct ggggcgtaac ggttccgact
     4141 attccgcgac acaaatcggt gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg
     4201 tcgccggggt atacagtgcc gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc
     4261 tgcgtctgga tgaagccagc gaactggctc gtctggcggc tcccgttctt cacgcccgta
     4321 ctttacagcc ggtttctggc agcgaaatcg acctgcaact gcgctgtagc tacacgccgg
     4381 atcaaggttc cacgcgcatt gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca
     4441 ccagccacga tgatgtctgt ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac
     4501 tggcgcataa agagatcgac caaatcctga aacgcgcgca ggtacgcccg ctggcggttg
     4561 gcgtacataa cgatcgccag ttgctgcaat tttgctacac ctcagaagtg gccgacagtg
     4621 cgctgaaaat cctcgacgaa gcgggattac ctggcgaact gcgcctgcgt caggggctgg
     4681 cgctggtggc gatggtcggt gcaggcgtca cccgtaaccc gctgcattgc caccgcttct
     4741 ggcagcaact gaaaggccag ccggtcgaat ttacctggca gtccgatgac ggcatcagcc
     4801 tggtggcagt actgcgcacc ggcccgaccg aaagcctgat tcaggggctg catcagtccg
     4861 tcttccgcgc agaaaaacgc atcggcctgg tattgttcgg taagggcaat atcggttccc
     4921 gttggctgga actgttcgcc cgtgagcaga gcacgctttc ggcacgtacc gactttgaat
     4981 ttgtgctggc aggcgtggtg gacagccgcc gcagtctgtt gagctatgac gggctggacg
     5041 ccagccgcgc gttagccttc ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt
     5101 tcctgtggat gcgcgcccat ccgtatgatg atttagtggt gctggacgtt accgccagcc
     5161 agcagcttgc tgatcagtat cttgatttcg ccagccacgg tttccacgtc atcagcgcca
     5221 acaaactggc gggagccagc gacagcaata aatatcgcca gatccacgac gccttcgaaa
     5281 aaaccgggcg tcactggctg tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca
     5341 ccgtgcgcga tctgatcgac agcggcgata ctattttgtc gatcagcggg atcttctccg
     5401 gcacgctctc ctggttgttc ctgcaattcg acggtagcgt gccgtttacc gagctggtgg
     5461 atcaggcgtg gcagcagggc ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag
     5521 acgtgatgcg caagctggtg attctggcgc gtgaagcagg ttacaacatc gaaccggatc
     5581 aggtacgcgt ggagtcgctg gtgcctgccc attgcgaagg cggcagcatc gaccatttct
     5641 ttgaaaatgg cgatgaactt aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa
     5701 tggggctggt gctgcgctac gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg
     5761 tggaagcggt gcgtgaagac catccgctgg catcactgct gccgtgcgat aacgtctttg
     5821 ccatcgaaag ccgctggtat cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc
     5881 gcgacgtcac cgccggggcg attcagtcgg atatcaaccg gctggcacag ttgttgtaat
     5941 ttcagaaatt taataatgcc cggtactcat gttttcgggt ttatggtttc taatgaaata
     6001 tattgaatta tcataggatt aggccggatt aagcgtttac gacgaatccg gcaagaagca
     6061 ataagtacat ggttagttta tatttgcagt ccggtttgct ttgcataccg gattttcttt
     6121 ttcttaccat cctgaagttt tttcatcttc cctgattttt cctcaccatc attggtcatt
     6181 tttcggttga cgcccttcgg cttttccttc atctttacat ctggacgtct aaacggatag
     6241 atgtgcacaa cacaacatat aactacaagc gattgatgag gtaaggtatg agcttttttc
     6301 acgccagcca gcgggatgcc ctgaatcaga gcctggcaga agtccagggg cagattaacg
     6361 tttcgttcga gtttttcccg ccgcgtacca gtgaaatgga gcagaccctg tggaactcca
     6421 tcgatcgcct tagcagcctg aaaccgaagt ttgtatcggt gacctatggc gcgaactccg
     6481 gcgagcgcga ccgtacgcac agcattatta aaggcattaa agatcgcact ggtctggaag
     6541 cggcaccgca tcttacttgc attgatgcga cgcccgacga gttgcgcacc attgcacgcg
     6601 actactggaa taacggtatt cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa
     6661 gtggtaagcc agaaatgtat gcttctgacc tggtgacgct attaaaagaa gtggcagatt
     6721 tcgatatctc cgtggcggcg tatccggaag ttcacccgga agcaaaaagc gctcaggcgg
     6781 atttgcttaa tctgaaacgc aaagtcgatg ccggagccaa ccgcgcgatt actcagttct
     6841 tcttcgatgt cgaaagctac ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg
     6901 tggaaattat tccgggaatt ttgccggtat ctaactttaa acaggcgaag aaatttgccg
     6961 atatgaccaa cgtgcgtatt ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg
     7021 atgccgaaac ccgcaaactg gtgggcgcga atattgccat ggatatggtg aagattttaa
     7081 gccgtgaagg agtgaaagat ttccacttct atacgcttaa ccgcgctgaa atgagttacg
     7141 cgatttgcca tacgctgggt gttcgacctg gtttataaat agtgtggctt ttgtgaaaat
     7201 cacacagtga tcacaaattt taaacagagc acaaaatgct gcctcgaaat gagggcggga
     7261 aaataaggtt gtcagccttg ttttctccct cattacttga aggatatgaa gctaaaaccc
     7321 ttttttataa agcatttgtc cgaattcgga cataatcaaa aaagcttaat tacgatcaat
     7381 ttgacctaca tctctttaac caacaatatg taagatctca actatcgcat ccgtggatta
     7441 attcaattat aacttctctc taacgctgtg tatcgtaacg gtaacactgt agaggggagc
     7501 acattgatga gcacgtcaga cgatatccat aacacgacag ccactggcaa atgcccgttc
     7561 catcagggcg gtcacgacca gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca
     7621 aatcaactcc gtgttgacct gttaaaccaa cattctaatc gttctaaccc gctgggtgag
     7681 gactttgact accgcaaaga attcagcaaa ttagattact acggcctgaa aaaagatctg
     7741 aaagccctgc tgacagaatc tcagccgtgg tggccagccg actggggcag ctacgccggt
     7801 ctgtttattc gtatggcctg gcacggcgca ggtacttacc gttcaatcga tggacgcggc
     7861 ggcgcgggtc gtggtcagca acgttttgca ccgctgaact cctggccgga taacgtaagc
     7921 ctcgataaag cgcgtcgcct gttgtggcca atcaaacaga aatatggtca gaaaatctcc
     7981 tgggccgacc tgtttatcct cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc
     8041 ttcggttttg gtgccggtcg tgaagacgtc tgggaaccgg atctggatgt taactggggt
     8101 gatgaaaaag cctggctgac tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt
     8161 gcaaccgaga tgggcctgat ttacgttaac ccggaaggcc cggatcacag cggcgaaccg
     8221 ctttctgcgg cagcagctat ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa
     8281 accgtggcgc tgattgcggg tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca
     8341 tcaaacgtag gtcctgatcc agaagctgca ccgattgaag aacaaggttt aggctgggcg
     8401 agcacttacg gcagcggcgt tggcgcagat gccattacct ctggtctgga agttgtctgg
     8461 acccagacgc cgacccagtg gagcaactat ttcttcgaaa acctgttcaa atatgagtgg
     8521 gtacagaccc gcagtccggc tggcgcaatc cagttcgaag cggtagacgc accggaaatt
     8581 atcccggatc cattcgatcc gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg
     8641 acgctgcgtt ttgatcctga gttcgagaag atctctcgtc gtttcctcaa cgatccgcag
     8701 gcgttcaacg aagcctttgc ccgtgcctgg ttcaaactga cgcacaggga tatggggccg
     8761 aaatctcgct acatcgggcc ggaagtgccg aaagaagatc tgatctggca agatccgctg
     8821 ccgcagccga tctacaaccc gaccgagcag gacattatcg atctgaaatt cgcgattgcg
     8881 gattctggtc tgtctgttag tgagcgggta tcggtggcct gggcatctgc ttctaccttc
     8941 cgtggtggcg acaaacgcgg tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc
     9001 gactgggatg tgaacgccgc agccgttcgt gctctgcctg ttctggagaa aatccagaaa
     9061 gagtctggta aagcctcgct ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag
     9121 aaagccgcaa gcgccgcagg tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat
     9181 gcgcgtcagg atcagactga cattgagatg ttcgagctgc tggagccaat tgctgacggt
     9241 ttccgtaact atcgcgctcg tctggatgtt tccaccaccg agtcactgct gatcgacaaa
     9301 gcccagcaac tgaccctgac cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta
     9361 ctgggtgcca acttcgatgg cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg
     9421 agcaatgact tcttcgtgaa cttgctggat atgcgttacg agtggaaagc gaccgacgaa
     9481 tcgaaagagc tgtttgaagg ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc
     9541 cgtgcggatc tggtatttgg ttctaactct gtcctgcgtg cggtggctga agtttacgcc
     9601 agcagcgatg ctcacgagaa gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg
     9661 aacctcgacc gtttcgacct gctgtaatct gaccttcttc agcgactgcc tttcaggcag
     9721 tcgctgaagt ttctttaccg gcgtatagtg tccacaggaa aactacacac tggatctctc
     9781 atgtctgccg caggaaagag caacccactg gcaatcagtg gcctggttgt gctcacactt
     9841 atctggagtt atagctggat tttcatgaag caagtcacca gctacatcgg tgccttcgac
     9901 tttaccgcct tacgctgcat tttcggcgct ctcgttttat tcatcgtcct tttattacgt
     9961 ggtcgcggaa tgcgcccgac accgtttaaa tacaccttag ccattgccct gttacaaacc
    10021 tgcgggatgg ttggcctggc gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg
    10081 atcctaagct ataccatgcc gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa
    10141 cgcctgcgac gtgggcaata tttcgcgata ctgattgccg ctttcggctt atttttggtg
    10201 ttgcagccgt ggcaactcga tttctcttcg atgaaaagtg ccatgctggc aatcctctcc
    10261 ggcgtcagtt ggggggcgag cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc
    10321 gtggatttat tgtcgttaac atcctggcag atgctctatg cggcgctggt gatgagtgtg
    10381 gtcgctttac tggtgccgca acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg
    10441 gcctacagtg cgattctggc gacggcactg gcgtggagct tatggttgtt tgtattgaaa
    10501 aacttgcctg ccagtattgc cagcttaagc acactggccg ttcccgtttg cggcgtactc
    10561 ttttcctggt ggctgctcgg cgagaatccg ggggccgttg aaggtagcgg tattgtgctg
    10621 attgtgatgg cactggcgct ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg
    10681 atctgaattt tttcttcatg tggggcgatc gcttatttaa caaaataacg ataatgcccc
    10741 accatgcgcc agctaaacag cacatcttct tcctgcgcac ctgcgccaat gttatgtatc
    10801 accagcggcg taccgtcgcg ggcgaagcca tctgaaacca ccccaatatg tgccagcccg
    10861 ttatctaatc gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctgatg
    10921 gggcgtgttt tatcgtggtg ggtaaaccag gtttccagat taggcacccg acggtgatcg
    10981 atgttgctgt ccgggcgctt taactgccat ttttgcgggt actcggcaaa attcttcgcc
    11041 atatcttcgt gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc
    11101 acatcggaac ataccccgcg tttttgcggg acatcaccgc caggataggt aagctgcata
    11161 tacgccggat cgtaaaatag cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca
    11221 aggttggtat tggcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag
    11281 tggctggtaa atgccgttag caggctgagc agcgccagtg aagctttcat ctggttcgtc
    11341 cgtgaaaata agagtcacag attcgacctt ccgggataac gattgttctg tagagaaaat
    11401 gtccagaagc ggataaaaaa ctcctgaaca acgtgggagt ttcagattac tggcacagag
    11461 tgccggatgc ggcgtgaacg ccttatctgt cctaaaaaac aagaaaattc aatatattgc
    11521 agagattgcg taggcctgat aagcgtagcg catcaggcaa ttttgcgttc aaactcccgg
    11581 acaagccggg agtttggagt aggttattcc cactcttgca ggaaacgctg accgtactgg
    11641 tcggctacca gcagagcggc gtaaacctga tctggcgtcg cgccgccagg catgttgtgg
    11701 atggtttcac attctgcaca tgccgcttca gccacaattc gcattttcgc cgggacatct
    11761 tctttaatat ccagttgagc gagagttatt ggcaaaccta ccgcatggct aagtgcagct
    11821 acggtttcga tttcctcaac cggtgcgttt tccagaacca gctgcgtcag cgtaccaaat
    11881 gccacttttt caccgtgata atagtgatgc gcgtccggga tagcggtcag gccgttatgc
    11941 actgcgtgcg ccgcagccag accaccactt tcaaaaccaa caccgctcaa ataggtgttc
    12001 gcttcaatca cgcgctccag cgccggagtc actacatgct gttcggcagc aagcatcgct
    12061 ttttcgcctt cttccagcag ggtgttgtag cacagttcag ccagtgccag cgcagcctgg
    12121 gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag agcaggcacg cgcttcaaac
    12181 caggttgcca gcgcatcgcc gatacccgcc gctaacagac gtgcaggtgc gccagcgacg
    12241 attttggtgt cgacaatgac catattcggg ttatttggca acagcagata gcggtcaaac
    12301 tcaccctcat cggtgtagat aacagacaat gcgctgcacg gtgcatcggt agaggcgata
    12361 gtcggtgcga tcgctaccgg aacacccatg aaatgtgcca gtgctttggc tgtatcgagg
    12421 gtttttccgc caccgatacc gagaattgcg ccacactgcg cagtctccgc gatgccacgc
    12481 agacggtcga tctcattttg cgaacatcca ccgccaaacg gcgcaatttc tactaccagt
    12541 ccagcatctt taaagctttt ctcgacagtg gattgagcaa aacctaaaac aaatttgtca
    12601 cccaccacta accagcgttc tgccagcggc ttcaggtatt cgcccagacg attaatcaca
    12661 tcagcgccct ggatgtattt acccggtgat tgaataatgc ggtccataat tgctccttta
    12721 gagatgagta gtgccaaatg cggcattcca gtcgtgttcg aacttctcta tagctgactc
    12781 taccgcaggg gtgttgagca tttgttgcgc tacatctaag ggcagggtga tggattcaca
    12841 tcctgccagt aaacagtcca gcgcctgacg cggcgtttta aagctggctg ccagcaccat
    12901 gctttctggc gcgtgcattt ctaacagcgc ttgcagctcc tgaaccgtac gaatgccgtc
    12961 tccgccctgg gcatctacgc ggttaacata cggagcaacg tattttgccc ctgccagcgc
    13021 ggccagtaac ccttgtgcag cactgtacac tgcggtgcca agcgtagtaa taccctcttt
    13081 tttcagtatt ttaattgctg ccagaccttc ggaagtcacc gggattttca ccacaatacc
    13141 cggaatagcg tcgcgcaggc gcttcgcttc ttccaccatc ccctgcgcgt cgcggctcat
    13201 ggtctgagca aacagaatgc cctcatcacc aatcgctttt tgcagacgcg gcagcacttc
    13261 ccatatggac tccttgctgg cagcgataat gctcgggtta gttgtcaccc cggcaatggg
    13321 gaatatgcgt gccagacgtt cgacttctgc gacgttagcg gtgtccagat acagttccat
    13381 gatgtttcct tttacagttc cagttcatgt tgcagcaggc tggcgatagc gtcggcagag
    13441 gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga tgcggcgagc gaggcgcgag
    13501 aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca gggttaacat gatgatgaat
    13561 tgcgcttcat catcacccca gcgcaccggc gcttgcagac gcgcaacgct gatggtggat
    13621 tgctcaatgt gttctgattt gctgtgtgga atggcaaaac taaagcccag accggtagag
    13681 aaaacggcct cgcgcgccca caagtcggct tccagtttac gcggatagcg gcagcgccct
    13741 gccagcagca ggttatcggt catgcctttg agcacttctt ctttgctgcg ccagtcgctt
    13801 tccagtgtga tgcactcggc ggtgaccagc ggtgcgtctt gttgggtcat gcggaattgc
    13861 gccagcaggt gctccacttc cagcgaagta cggcaggcca ttgcctggtt gagcaacttg
    13921 cggcactcac ggctatcaag ttgcgccatc cgcgctttcg ccgccggaat tgatggtgcg
    13981 ctcatgctga gttcatccag ccctaagccg accagcaacg gcagcacgga acctttcgct
    14041 cccagctcac cgcacagacc aatccattta ccctggcgat gcaccgcctg cacggcgtaa
    14101 tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt gacgagtaac cttagcgtta
    14161 tcgcgatcca ctgccagcag atattgcgtc aggtcattac taccaatact aaagaaatca
    14221 atctcttcgc agcattgatc gatgatgaac atcaccgacg gcacttccag cataatgccg
    14281 agctggatct tctcatcaaa cggaatgtgt tcgttacgta gttgctgttt ggcttctgcc
    14341 agtttttctt tcacccataa gatctcttcc attgaggaga tcatcgggat catgattttc
    14401 aggctgccgt gagcggaggc gcgcaggatc gagcgtagtt gtgtggtaaa taacgacgcg
    14461 tattcttcat aaatacgcac ggcgcgataa ccgaggaacg ggtttgcctc tgcgggaatg
    14521 tttagataat caacgggttt gtcgccgcca atgtccatag tgcgcacaat aatgctgcgt
    14581 ccgttggcag actccagcgc ctgacaaaaa atgttgtaca gctcgctttc gcccggtgca
    14641 ctggtgcgat ccatatagag catttcagtg cggaacaaac caacgccttc cgcaccattg
    14701 ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag cggcaatttc aatgcggata
    14761 ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct gttgctcacg cagggcgtcc
    14821 tgtacgcggg cttcttgctg ataataacga gctacggctt ctcctggctc aaccacaatc
    14881 gccccggcgt tgccgtcgat ataaatcgtt tgatgctgcc acggagtgag ggcatcaata
    14941 tccacaccaa ccagcgtcgg aatgttgaac gaacgggcaa ggatcaccgt atgtgaggtg
    15001 gtgccgccac ttttcagcaa cagacctttg aggtgatttt tatccagttc gaggaactgg
    15061 ctgggggtca gttcatcagc catacaaatg gcggactgcg tcagtttgcc cggtgccggg
    15121 aagcgttgct caccgtagat ttgctggagt aactggaagc agacgtcgcg tacgtccagg
    15181 gcacgttctt gcaggtagct gctgctggaa cgggcgaact cttcgcaaaa gtgattcgca
    15241 ctggcgacaa tcgcttcggc gcagcttaat ccctcgctga cgcctgccag taagtgctga
    15301 cgtaaagatg tatcgccagc cagggatcgg tgagcttcca gtatcgcgct ggtagcaccg
    15361 tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca gcgtcaggcc gttttccagt
    15421 gtggattgct cggcgtcaac gtctttggct gcgggaagat tactcagcgc attgagatct
    15481 aaagaagaga tcggcgtcag aatgccgcct gcactaccgc tgcacacggt gcgggcgcgg
    15541 ataatttgcg gattcagatt ggtcagtgaa accggcagtg gttccagttc gtcagattta
    15601 acttccgcca gcggcgcatc gcagtggggg aattcatcgc gcagccattg gcttaaacgc
    15661 tggtgcgctt cctgttcgtc ggccccggaa atcagtaact ggcagttatc gcccgccagc
    15721 gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc ccttgcggtc agtgtgcagg
    15781 ttatgccact caatttgtga tgaaaaagta ttacacagcg tttcaacgtg gcttgccgga
    15841 cgcgcatgta cgccgttagg tagctcacaa ataaattcca caatcagggc cattgcctct
    15901 ctcccggtac ggttttctgt ttacaggata agagcgcacg gcaacggcct gccatgtgac
    15961 aaatctgcca aaagctggac aaatgtaatg taaccgtcaa tttgcgacgc gtctcacaag
    16021 acgccgtttt gcggcatgct tccggtttat cgcaagttat gaggcggatc gcatttttgt
    16081 actgatatta caaaagtcca gtaaatggcc tttttatcca ctgtttgctc cgcctgcgat
    16141 tgcctattgt ttcctccatc aaaaatatgg acatgggctt tcgcaagccc gggagcagct
    16201 tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag catttaatga cgggcgtttc
    16261 acacatgatt cccttcgtgg tatcgggcgg tattttgctg gcggtttccg tcatgttgta
    16321 tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat ctgaaaaaac tgtttgatat
    16381 cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgcc gcttacattg gttattccat
    16441 tgcagaacgt tctgcgctgg ctccatgcgc tatcggtacc tgggttggta acagctttgg
    16501 tgcgggcttc tttggtgcac tgatcgccgg gattatcggc ggcatcgtgg tgcattacct
    16561 gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg cctatcttca ttattcctat
    16621 cgtcggcaca ctgattaccg caggcatcat gatgtggggc ttgggcgagc ctgtaggggc
    16681 gttgaccaac agcctgactc agtggcttca ggggatgcag cagggcagca ttgttatgct
    16741 ggcggtgatc atgggtctga tgctggcgtt cgatatgggc ggtccggtta acaaagtggc
    16801 ctatgccttc atgctgattt gcgttgctca gggtgtttat accgtggtgg ccatcgctgc
    16861 cgtgggtatt tgtatcccgc cgctggggat ggggctggcg acgctgattg gtcgtaaaaa
    16921 tttctccgca gaagagcgcg aaaccggcaa agcggcactg gtgatgggct gtgttggcgt
    16981 tactgaaggg gcgattcctt tcgccgctgc cgatccgctg cgtgtcattc cttccatcat
    17041 ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc ggtgcgcagt gctatgcagg
    17101 ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag ctgggttata tcgcagcagt
    17161 ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg ctgaaaagtc tggcgcgtaa
    17221 aaatgggtct tcgactgatg aaaaagaaga cgacctggat ttggattttg aaattaatta
    17281 attgaggaac gagccatgac gaaaattatt gcagtaaccg catgtccttc aggtgttgtc
    17341 catacctata tggcagcaga ggcgctggaa agtgccgcga aagcgaaagg ctgggaagtg
    17401 aaagtagaaa cctaggggtc aattggtctg gaaaatgaac tgactgcgga agatgtggcg
    17461 agcgctgata tggtgattct gaccaaagat atcggcatca agtttgaaga gcgttttgct
    17521 ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa aacgtgctga cgccattatg
    17581 agcaaaattg aagctcacct ggcgcaaacc gcttaagttc ttctcccgct cgcaagggcg
    17641 ggttcgcttt cccacaggag ttcctcatga cgaatcgtat ctctcgcctc aaaactgcac
    17701 tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct gctttatacc gccagccatc
    17761 ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa agcaacagcg tatatccttg
    17821 aacatgttga aatttcgatt cgtgatgaag aactgattgc cggtaaccgc accgtaaaac
    17881 cgcgcgccgg gattatgtcg ccggaaatgg acccttactg gctgctgaaa gagctggatc
    17941 aattcccgac gcgtccgcag gaccgctttg ctatcagcga agaagataaa cgtatctacc
    18001 gtgaagagtt gttcccgtac tgggaaaaac gttcgatgaa agatttcatc aacgggcaga
    18061 tgacagatga agtaaaagcc gcgaccaaca cgcagatttt cagcatcaac cagacggata
    18121 aaggccaggg gcacattatt attgattacc cacgcctgtt gaatcacggt ctgggtgagc
    18181 tggtggcaca gatgcagcaa cattgtcagc aacagccgga gaatcacttt tatcaggccg
    18241 cgttgttact gctggaagcc tcgcagaaac acattttgcg ttacgccgaa ctggcggaaa
    18301 cgatggcggc aaactgcaca gatgcacagc gtcgcgaaga actgctgact attgcggaga
    18361 tctctcgcca taacgccgaa cataagccgc agacgttctg gcaggcgtgc cagttattct
    18421 ggtacatgaa catcattctg caatacgaat ccaacgccag ttcgctgtct ttagggcgct
    18481 tcgaccagta tatgttgccg ttctatcagg catctttaac ccagggcgaa gatccggcgt
    18541 tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa cgacatcgtg ctgttgcgct
    18601 ccaccagcag cgcgcgttat ttcgcaggtt tcccgaccgg ctataccgca ctgctcggcg
    18661 ggttaaccga gaacggacgt agcgcggtga acgtgctttc gttcctttgc cttgacgcct
    18721 atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg cactaacgcc ttgatcgaca
    18781 cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg caccggtatt ccgcaaatct
    18841 ttaacgatga agtggtggta ccagcgttcc tcaaccgtgg cgtttcgctg gaagatgcgc
    18901 gcgactattc cgtagtgggc tgtgtggaat tatctattcc cggcagaacc tacggcttgc
    18961 atgacatcgc gatgtttaat ctgctgaaag tgatggaaat ctgcctgcat gaaaatgaag
    19021 gcaatgctgc gctgacttat gaaggtttac tggagcagat ccgcgccaag atcagccact
    19081 acatcaccct gatggttgag ggcagcaata tttgtgatat cggccatcgc gactgggcac
    19141 ctgtaccgct gctctcgtct tttatcagcg attgtctgga aaaaggtcgc gatattaccg
    19201 atggcggcgc gcgttataac ttctccggcg tacaggggat cggtatcgcc aacctgagcg
    19261 attctctcca tgcgttgaaa gggatggttt ttgagcaaca gcgtttaagt tttgacgaat
    19321 tgctgtcggt attaaaagcc aactttgcaa cgccagaagg cgaaaaagtc cgtgctcgct
    19381 taattaaccg cttcgagaaa tacggtaacg atatcgacga ggtggataac atcagcgccg
    19441 aactgttacg ccactactgc aaagaagtgg aaaaatacca gaacccgcgc gcggttactt
    19501 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    19561 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    19621 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    19681 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    19741 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    19801 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    19861 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    19921 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    19981 gcgatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    20041 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    20101 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    20161 aatgtttgcg tgatgcggat gaatgcccct ccggggcgtt tgaacggatt ggtcgcgata
    20221 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    20281 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    20341 ttttacagcg actgcggctg tggggtgtgt catgcgccat tgaaactgcc ggagacgcgc
    20401 cagcaagcaa gctgttaccg ctggcgaaat tgtgcgatga agtgctgttc gatttaaaaa
    20461 ttatggacgc gactcaggcg cgggatgtgg tgaagatgaa cctgccacgc gtgctggaga
    20521 atctgcgttt gctggtgagt gagggtgtca acgtgatccc gcgtttaccg ttgatccctg
    20581 gtttcacgct cagcagggag aatatgcagc aggcgctgga tgtactgatc ccgctgaaaa
    20641 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    20701 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    20761 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    20821 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatactt atatggcggc ggaacggctg
    20881 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgctggga
    20941 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    21001 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    21061 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgcg
    21121 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    21181 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    21241 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    21301 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    21361 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    21421 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    21481 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    21541 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    21601 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    21661 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    21721 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    21781 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    21841 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    21901 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    21961 agagcagata gctgacgtcg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    22021 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    22081 aacattgcgg cagttgtatt gccggatgtg gcgtaaacgc cttatccagc ctaccgccgc
    22141 gatctgtagg ccggataaga cgcgtcaagc gtcgcatccg gcaaatagcg cctggctgat
    22201 aattactgat tacctacctg gtcgccatac ggcagagtgt cgtaatcgat cagcgcgttt
    22261 ttcttgtacg ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa
    22321 cgggttggat cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt
    22381 tccgccaggc tgtatttacg atcgacatcc tgtgagaaat cacggggatg agtcgcttgc
    22441 catttttccg acgtccacag caggaacgga atggtgtaca tgtggcgcgt cgggttgtct
    22501 tcgttacgcc cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag
    22561 aagtaaacca ggaatccgtt cgggtctgcc gctttaaagt ctttaatcag gctggcaacc
    22621 acatgatcgt tatacaggtt agcgttgtca taatcgttat atgattccag ctcttctgcg
    22681 cttaatcctg gcggaacatg atcggtattg ccatcaaact tgccctgatt ttccgggtag
    22741 cggtatttgt atttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca
    22801 gggtcattca gcacatcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc
    22861 tgcgtacgtt gctggttcat gtagtactgc ttgtcggtct ggcgcgaaaa taccgtcagc
    22921 atggtattgc gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc
    22981 tgtttcatca tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg
    23041 gcaaaggtca gcgcctgttg caggatttca atggtgtacg gacgagacgt aactacgtta
    23101 ttaaacacgg tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca
    23161 cgcggataac cgtacagact catgcgtccg cgctgggtcg actcgccaat caccagcact
    23221 aaagtgcgcg gttcgttacc cgattcatct ttgaaattag ccagtggcgg caaggcatta
    23281 ttttcattca gtaactttgt tagcgagttt agttgctgac gatactgata atagccggtc
    23341 aggaattgcc acggtgcggc aggctccata cgcgaggcca ggttatccaa cgttttctca
    23401 aacggcttgt ttttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc
    23461 agggcaaaag agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc
    23521 cacagcagaa ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa
    23581 tactggctta aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg
    23641 ctctgcgaga actcctgccc gtagatgacg tagtagcata gcgccgccag agaggccgcc
    23701 catagcacca cgccgattac tgcggcaata attttaatcc gcttcggaaa gaggaatacc
    23761 gggatcaacc acagcgagct gaataacagc gagtcgcgaa tgccgttggt gccactataa
    23821 ccactgatgt aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc
    23881 caacccaggg ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta
    23941 agaacctgtc ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa
    24001 ggtatattca gaatttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga
    24061 acagaagtat ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagaa gaggaagatt
    24121 agccggtatt acgcatacct gccgcaatcc cggcaatagt gaccattaac gcttgttcga
    24181 cgcgaggatc cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg
    24241 cctgcaatac gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc
    24301 acggcagatc ggccatcaga tgggaatcgt tggcaatcgc cagcaccact ttgatgtctt
    24361 cttcttgcag gttgcgtaac tctttaccta acggccacag tgctttgtct accagacgtt
    24421 gatcatagta ttccgccaac cacaggtctg ctttggcgaa gaccatctcc agcatgccga
    24481 gacgcgtcga gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttgccat
    24541 cttcgaccac tttttgcagc gccgtacctg cacccagcca ggcggggagc atcaggcggt
    24601 tttgcgtcca ggcaaaaatc cacggtatgg cgcgcagcga ctcgacgccg ccggttgggc
    24661 gacgtttcgc cggacgtgaa cccaacggca gtttgcccaa ttcttgttcc ggcgtagcgg
    24721 agcggaagta aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg
    24781 agatgactga cagttcatcc ataatgcgac gccagctctc tttcggctca ggtggtggca
    24841 gcaggttggc ttccagaatc gccccggtgt aaagcgacag gctgctgacg gtgatttctg
    24901 gcagaccata tttaaagcgg atcatctcgc cctgttcagt cacgcgcagg ccacctttca
    24961 ggcttcctgg cggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg
    25021 aaccgccgcg accgtggaac aacgtcagct caatacccgc tttttcgcag gttttgatta
    25081 atgcatcctg tgcctgatat tgcgcccagg aagctgccat cacgcccgca tcttttgctg
    25141 agtcggaata gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga
    25201 tattgagcag ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca
    25261 gcggagcaac cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca
    25321 gtacgtcgga cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg
    25381 cttcggcaat cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt
    25441 tgcgcggcag aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg
    25501 cctctgacca gctttcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt
    25561 cggtatggcg ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt
    25621 tcacgcggcg cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct
    25681 gaagtgactg gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcagcc
    25741 cttctggttt tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca
    25801 tcaggcgaga acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc
    25861 caaccagcgc cagcagttca ggggtcgctt caaccatcga cagttcagaa accagaacct
    25921 gaatatcttt caggaacaga tcggtggctt tccagcgact gagtagcagg acgtggcggg
    25981 tgatatcggc agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaagtaaaac
    26041 ggaccggaac aaattcgacg ggcagtttgt agccgaggtt ctcttccagt tgttcgttca
    26101 gttcgcgcaa gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc
    26161 atttggcttc atctaccggg cttggacgca gcttacggat ttcatcagta tgccatgact
    26221 gggcgattaa ctggcgcagg cgacgcatca gctggttgtg ttcgtagtca gcgatatctt
    26281 tgttatcgag ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac
    26341 gggtaatttc ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt
    26401 ttttgatggt gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga
    26461 tcacttccgg gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg
    26521 tgttggctag gttcaggaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg
    26581 acaaattttg taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag
    26641 atttcgacaa cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct
    26701 tgatggtttc tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt
    26761 gttcgttcat attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg
    26821 tcgcattggc gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa
    26881 aagcggtgac gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta
    26941 cgccattaaa ttcacgacgc tttaaataag cgtaacttat ggaaatgtta aaaaatcgcc
    27001 atacataaca ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattatt
    27061 gcggcaccgg agtttggcaa cggtgccgga tgcggcgcga gcgccttatc cggcctacgt
    27121 tttaatgcca gcaaaaatgg tgaattacct ggataatcaa ctcacgggtg ggtttaataa
    27181 accgcgtctc cagatattca tcaggctgat gagcttgatt gatggaaccc ggaccgagca
    27241 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    27301 tttttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    27361 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgttcgctca
    27421 ccggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg ccaggcagcg
    27481 gacgaatatc catatgcaac tcacagcaag cgcaaatacg gttagaagcg tcgccaccgt
    27541 gaatatgccc gaggttgagc gtagggtatg gcacggtaaa tgcttcgtag tgataacgtt
    27601 ctttcaggtt atcgcgcaat tgcaaaatat gcccgatggc gtcgtgcatt agttcgatag
    27661 cgttaactcc gcgtgctgga tcgctggagt gccccgactg gccctgaata cggatggcgt
    27721 tagagatatg acctttatgt gcgcgtaccg gttgtagtga cgtcggttcg ccaatgatgg
    27781 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    27841 cttcatcagc agtcgccaga atgtagagcg gttttttcag tttcgtgacg tcgacatcgc
    27901 gtagcgtatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    27961 gcttgccgtc atgctccgtc agtgtaaacg gatcgcgcgt ccagcgaccg tcatcaaatg
    28021 gcaccgtatc ggtatgcccc gccagcaaca agccgccagc cccctgtccg atacttgcca
    28081 gcatattgaa tttgttgcga gttcctggca caggctgcac ttccacattg aagcctaaat
    28141 ctttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    28201 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    28261 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    28321 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    28381 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    28441 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    28501 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    28561 ctccgatttg catccgcagc taaaaggcat cgttgagctg ccgttgcagc cgatgtcgga
    28621 tatcagcgag tttagcccag gggtggacgt agtgtttctc gccaccgccc atgaagttag
    28681 ccacgattta gcgccgcagt ttcttgaagc gggctgcgtg gtgttcgacc tttccggtgc
    28741 gtttcgtgtt aacgacgcca ccttctatga aaaatattac ggctttaccc atcaataccc
    28801 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    28861 agcgaatttg attgcggtgc caggctgtta tccgacggcg gcacagctgg cgctgaaacc
    28921 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    28981 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    29041 accgtatggc gtctttactc atcgccatca accagagatc gccacacacc tcggtgctga
    29101 cgttatcttc accccacatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    29161 ccgcctgaaa tcgggtgtga cccaggcgca agtcgcgcaa gtgttacagc aggcgtatgc
    29221 ccataaaccg ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    29281 gctgccattt tgcgatatcg ggtttgccgt tcagggcgag catctgatta ttgtggcgac
    29341 cgaagacaac ttactgaaag gcgcggcggc acaagcggta cagtgcgcca atattcgttt
    29401 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    29461 aactgggcgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcacactgg
    29521 tgaattatcg tgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    29581 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaacggc ctgcgggtga
    29641 cgcctgctga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    29701 tgttggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    29761 acagcgtcaa agtgacccag cttgatgaag agttaggtca tgttggactg gcgcagccag
    29821 gttcgcctaa gcttatcaac tccttgctgg agaacggtta tctgccggtg gtcagctcca
    29881 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcaacggcgc
    29941 tggcggcaac gctgggcgcg gatctgattt tgctctccga cgtcagcggc attctcgacg
    30001 gcaaagggca acgcattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    30061 gcattattac tgacggcatg atagtgaaag tgaacgcggc gctggatgcg gcccgcacgc
    30121 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga gcagcttccg gcactgttta
    30181 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    30241 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    30301 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    30361 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggtc acggtaggcg
    30421 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gaacgtgctg ctggaagatg
    30481 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    30541 aaggcaaact gatcgacaaa gtgggccagt taggcaaaaa gctgcatacc gggcgtagtc
    30601 gtaacgatca ggtagcgact gacctgaaac tgtggtgcaa agataccgtt agcgagttac
    30661 tgacggctaa ccgacagctg caatcggcgc tggtggaaac cgcacaaaac aatcaggacg
    30721 cggtcatgcc aggttacact cacctgcaac gcgcccagcc ggtgacgttc gcgcactggt
    30781 gcctggccta tgttgagatg ctggcgcgtg atgaaagccg tttgcaggat gcgcttaagc
    30841 gtctggatgt cagcccatta ggctgtggcg cgctggcggg aacggcctat gaaatcgacc
    30901 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctggacagcg
    30961 tttctgaccg tgaccatgtg ttggaactgc tgtcggctgc cgctatcggc atggtgcatc
    31021 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    31081 tttctgaccg cgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    31141 tggagctgat tcgcggtaaa tgcggtcggg tgcagggtgc gttaaccggc atgatgatga
    31201 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    31261 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    31321 ttcaggtgaa acgtccacgt tgccaggaag cggctcagca gggttacgcc aacgccaccg
    31381 aactggcgga ttacctggtg gcgaaaggcg tacctttccg cgaggcgcac catatcgtgg
    31441 gtgaagcggt ggtggaagcc attcgtcagg gcaaaccgct ggaagatctg ccgctcgacg
    31501 agttgcagaa attcagcccg gtgattggcg aagatgtcta tccgattttg tcgctgcaat
    31561 cgtgcctcga caagcgtgcg gcaaaaggcg gcgtctcacc gcagcaggtg gcccaagcga
    31621 ttgcttttgc gcaggcgcgg ttggggtaag aacatttata tgtacaaatt tgagcctggc
    31681 ttatcgccgg gcttttttat ggcaaaaaaa agcggatcct ggagatccgc aaaagttcac
    31741 gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt caagggttaa
    31801 aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat agggataatc
    31861 gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat ggataatgaa
    31921 tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc ggcgtgcggc
    31981 agattcctgc cacgttagcc agccgacgct tagcgggcaa attcgtaagc tggaagatga
    32041 gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat
    32101 gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc
    32161 aagccagcag ggcgagacga tgtccggacc gctgcatatt ggtttgattc ccacggttgg
    32221 accgtacctg ctaccgcata ttatcccgat gctgcaccag acctttccaa agctggaaat
    32281 gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagtg gcaaactcga
    32341 ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc cgttgtttga
    32401 tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtacc
    32461 gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact gtttgcgcga
    32521 tcaggcaatg ggtttctgtt ttgaagccgg ggcggatgaa gatacacact tccgcgcgac
    32581 cagcctggag acgctgcgca acatggtggc ggcaggtagc gggatcactt tactgccagc
    32641 gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccgt gcattaagcc
    32701 ggaaccacgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc gcagccgcta
    32761 tgagcagctg gcagaggcca tccgagcaag aatggatggc tatttcgata aagtgttaaa
    32821 acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatg gtcgggtagt
    32881 taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt tccataatcg
    32941 cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata cccagaatct
    33001 cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg acgatttgtg
    33061 cgcgtgccag atgtttaaac tgggcgcggc ccacttcata tggcactttc atcgcggtca
    33121 gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg ccggtaggga
    33181 tatcttcaat cagatgcgcg gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc
    33241 cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc accgcgtaaa
    33301 cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt
    33361 ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg ttggcataga
    33421 gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca ccgtcgtcac
    33481 agccttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg ttccagaagt
    33541 gataggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt
    33601 tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca cagccgatca
    33661 ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga attgagtcgc
    33721 tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga cgagagccgc
    33781 aggcaataac aaatttttca gcggttagtg tttcgacgct gccgtccggg caatccagcg
    33841 ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag tgattacgtt
    33901 cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca tggttaagga
    33961 tatcggcaaa agaagagcgg agcagtcgcg aatggtcgct gtatagtggg ttttgattga
    34021 attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg ccccagtggg
    34081 tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcaca ccttgcttaa
    34141 ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg gcatcgtaat
    34201 cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa cagaatggta
    34261 acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata tcctgagcgg
    34321 cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt tatcgacgat
    34381 actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag taaatatgtt
    34441 ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat tctctggtat
    34501 agtgccagca gtacttttgg caaggattca gacatcgtga tgggcataag agcgcaacaa
    34561 aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag tgctgaacgc
    34621 agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct
    34681 ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga tgagagcggt
    34741 ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg cgggagtgtg
    34801 atccgcacct cggtctccac atttatggag ttcatcggta ataatcctaa cgccttccgg
    34861 ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa
    34921 attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca tatgccgcgt
    34981 gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag
    35041 gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt actgcaactg
    35101 cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa aaccgcaatt
    35161 attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga ggtacgctgc
    35221 tgctggcgtt agttgctggc ttatcgatta atggtacttt cgcagcgctg tttagctcca
    35281 ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac tgcctgcatc
    35341 aacgttatct taatcgcact atgccggtag gcttgccggg tctggcagct gcctgtttta
    35401 ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat atcggctcta
    35461 acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg aagatgcgta
    35521 accgtaagca ggaagttgct gagtaatcgg tattatgccg gatgctggcg catccggcat
    35581 gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta ggggaactga
    35641 tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag attcttgcat
    35701 aacgtttccg ggttacagga gatgtacaaa atacacggat acgcctgcac cattttctcg
    35761 gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgca ctgataactc
    35821 tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat
    35881 tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagctgc gatgttgtat
    35941 tgcgcagcag caaccgacgg cttagcgatt tcggtggcta atacccgatc aaaattgcgc
    36001 gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc gccttttgag
    36061 cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc gttcggctgg
    36121 gtaaagctgt tttctacctg acggtagatc atctctttcc ctgcgaccgg cagacgctcg
    36181 tcgatgtaat cttgatccag ctcgattttg gttttcgttg cccgaccaat cagatgcaca
    36241 ttcagattct gcgcgcgcag tgcatcgcgc agggcctccg cttcctgacg ccactcatca
    36301 tccagcttct tatggtatag cagggaaacc accgcctgat tactcagcgt ggtgaggtaa
    36361 tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc aatcatcgcc
    36421 gtcatcaact ggttgataag ttcactggcg gcggggaagc tatccacgcg gatacggctt
    36481 ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca gatgcggaac
    36541 tccgcacgca tccggtaatg actgaccggc gagcgaaaca cttccggaac caggtcagaa
    36601 aatggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc ttcatactgt
    36661 tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt
    36721 gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga gctacgtctg
    36781 gacagtaact tgttacaacc tgtagcatcc acttgtcggt cctgtgagtt aatagggaat
    36841 ccagtgcgaa tctggagctg acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc
    36901 ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc cctccaagcc
    36961 cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc
    37021 tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt cgctgctgac
    37081 ggcgtgttct gtcacagcct tttccgcttg ggcacaggat accagcccgg atactctcgt
    37141 cgttactgct aaccgttttg aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt
    37201 gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct
    37261 tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta tttttattcg
    37321 cggtacaaat gccagtcatg tgttggtgtt aattgatggc gtacgcctga atctggcggg
    37381 ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc gtgttgaata
    37441 tatccgtggg ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg tggtgaatat
    37501 catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa gcaatagtta
    37561 tcagaactat gatgtctcta cgcagcaaca actgggggat aagacacggg taacgctgtt
    37621 gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata caggaacgca
    37681 agcgcagaca gataacgatg gttttttaag taaaacgctt tatggcgcgc tggagcataa
    37741 ctttactgat gcctggagcg gctttgtgcg cggctatggc tatgataacc gtaccaatta
    37801 tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct atagccaaag
    37861 ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca ttaccagcta
    37921 tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt cgtcggcgac
    37981 gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg
    38041 tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta caggttatgt
    38101 tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc aacaagtcgg
    38161 cgattttacc tttgaaggcg cagcacgcag tgacgataac tcacagtttg gtcgtcatgg
    38221 aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca ttgcttccta
    38281 cgggacatct tataaggcac caaatctggg gcaactgtat ggcttctacg gaaatccgaa
    38341 tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa ccgctggggt
    38401 gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt atgatgatca
    38461 caccctgaaa tattacaacg aagggaaagc gcggattaag ggcgtcgagg cgaccgccaa
    38521 ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc
    38581 gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc agctcgactg
    38641 gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc gctatgataa
    38701 ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct
    38761 tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag ccaacctgtt
    38821 cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat acaccttgtc
    38881 tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg
    38941 ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac atttatgctt
    39001 tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg
    39061 tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg gtcgcttgca
    39121 acactgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg
    39181 gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt gtcggattac
    39241 tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg cgtttcgcta
    39301 atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct gaagcgaagc
    39361 tacatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg tggttaagaa
    39421 tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta ctacaagaag
    39481 aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca gcgattgctc
    39541 gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc gatgcgaata
    39601 ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt ttacagcgtt
    39661 acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga atgttgcgcg
    39721 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    39781 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaatct
    39841 caacagagaa aagcaaaaaa aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
    39901 tcgcgccgct gagaaaaagc gaagcgg
//
LOCUS       DAZRVY010000024        39790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZRVY010000024 DAZRVY010000000
VERSION     DAZRVY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.024"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHXOX_001308"
     CDS             <1..435
                     /locus_tag="ACHXOX_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR7004827.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHXOX_001309"
     CDS             518..1420
                     /locus_tag="ACHXOX_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR7004828.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHXOX_001310"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHXOX_001310"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR7004829.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHXOX_001311"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHXOX_001311"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR7004830.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHXOX_001312"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHXOX_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR7004831.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHXOX_001313"
     CDS             complement(5330..6037)
                     /locus_tag="ACHXOX_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR7004832.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHXOX_001314"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHXOX_001314"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR7004833.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHXOX_001315"
     CDS             complement(6895..7098)
                     /locus_tag="ACHXOX_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7004834.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7299..7441
                     /locus_tag="ACHXOX_001316"
                     /pseudo
     CDS             7299..7441
                     /locus_tag="ACHXOX_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7603..7923
                     /gene="cyaY"
                     /locus_tag="ACHXOX_001317"
     CDS             7603..7923
                     /gene="cyaY"
                     /locus_tag="ACHXOX_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR7004835.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7963..10509)
                     /gene="cyaA"
                     /locus_tag="ACHXOX_001318"
     CDS             complement(7963..10509)
                     /gene="cyaA"
                     /locus_tag="ACHXOX_001318"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR7004836.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10896..11837
                     /gene="hemC"
                     /locus_tag="ACHXOX_001319"
     CDS             10896..11837
                     /gene="hemC"
                     /locus_tag="ACHXOX_001319"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR7004837.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11834..12574
                     /gene="hemD"
                     /locus_tag="ACHXOX_001320"
     CDS             11834..12574
                     /gene="hemD"
                     /locus_tag="ACHXOX_001320"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR7004838.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            12596..13813
                     /gene="hemX"
                     /locus_tag="ACHXOX_001321"
     CDS             12596..13813
                     /gene="hemX"
                     /locus_tag="ACHXOX_001321"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR7004839.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            13816..15012
                     /gene="hemY"
                     /locus_tag="ACHXOX_001322"
     CDS             13816..15012
                     /gene="hemY"
                     /locus_tag="ACHXOX_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR7004840.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15674..15811
                     /locus_tag="ACHXOX_001323"
                     /pseudo
     CDS             15674..15811
                     /locus_tag="ACHXOX_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(15919..17154)
                     /locus_tag="ACHXOX_001324"
     CDS             complement(15919..17154)
                     /locus_tag="ACHXOX_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR7004841.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17301..17377)
                     /locus_tag="ACHXOX_001325"
     tRNA            complement(17301..17377)
                     /locus_tag="ACHXOX_001325"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17341..17343),aa:Pro,seq:tgg)
     gene            complement(17420..17506)
                     /locus_tag="ACHXOX_001326"
     tRNA            complement(17420..17506)
                     /locus_tag="ACHXOX_001326"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17470..17472),aa:Leu,seq:cag)
     gene            complement(17527..17602)
                     /locus_tag="ACHXOX_001327"
     tRNA            complement(17527..17602)
                     /locus_tag="ACHXOX_001327"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17567..17569),aa:His,seq:gtg)
     gene            complement(17661..17737)
                     /locus_tag="ACHXOX_001328"
     tRNA            complement(17661..17737)
                     /locus_tag="ACHXOX_001328"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17701..17703),aa:Arg,seq:ccg)
     gene            complement(17840..19225)
                     /locus_tag="ACHXOX_001329"
     CDS             complement(17840..19225)
                     /locus_tag="ACHXOX_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7004842.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19416..20156)
                     /gene="wecG"
                     /locus_tag="ACHXOX_001330"
     CDS             complement(19416..20156)
                     /gene="wecG"
                     /locus_tag="ACHXOX_001330"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR7004843.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20159..21511)
                     /gene="wzyE"
                     /locus_tag="ACHXOX_001331"
     CDS             complement(20159..21511)
                     /gene="wzyE"
                     /locus_tag="ACHXOX_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR7004844.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21508..22587)
                     /gene="rffT"
                     /locus_tag="ACHXOX_001332"
                     /gene_synonym="wecF"
     CDS             complement(21508..22587)
                     /gene="rffT"
                     /locus_tag="ACHXOX_001332"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR7004845.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22584..23834)
                     /gene="wzxE"
                     /locus_tag="ACHXOX_001333"
     CDS             complement(22584..23834)
                     /gene="wzxE"
                     /locus_tag="ACHXOX_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR7004846.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23836..24966)
                     /gene="rffA"
                     /locus_tag="ACHXOX_001334"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23836..24966)
                     /gene="rffA"
                     /locus_tag="ACHXOX_001334"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR7004847.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24971..25645)
                     /gene="rffC"
                     /locus_tag="ACHXOX_001335"
                     /gene_synonym="wecD"
     CDS             complement(24971..25645)
                     /gene="rffC"
                     /locus_tag="ACHXOX_001335"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR7004848.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25623..26504)
                     /gene="rfbA"
                     /locus_tag="ACHXOX_001336"
     CDS             complement(25623..26504)
                     /gene="rfbA"
                     /locus_tag="ACHXOX_001336"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR7004849.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26523..27590)
                     /gene="rffG"
                     /locus_tag="ACHXOX_001337"
     CDS             complement(26523..27590)
                     /gene="rffG"
                     /locus_tag="ACHXOX_001337"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR7004850.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27590..28852)
                     /gene="wecC"
                     /locus_tag="ACHXOX_001338"
     CDS             complement(27590..28852)
                     /gene="wecC"
                     /locus_tag="ACHXOX_001338"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR7004851.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28849..29979)
                     /gene="wecB"
                     /locus_tag="ACHXOX_001339"
     CDS             complement(28849..29979)
                     /gene="wecB"
                     /locus_tag="ACHXOX_001339"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR7004852.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30035..31081)
                     /gene="wzzE"
                     /locus_tag="ACHXOX_001340"
     CDS             complement(30035..31081)
                     /gene="wzzE"
                     /locus_tag="ACHXOX_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR7004853.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31093..32196)
                     /gene="wecA"
                     /locus_tag="ACHXOX_001341"
     CDS             complement(31093..32196)
                     /gene="wecA"
                     /locus_tag="ACHXOX_001341"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR7004854.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32436..33695)
                     /gene="rho"
                     /locus_tag="ACHXOX_001342"
     CDS             complement(32436..33695)
                     /gene="rho"
                     /locus_tag="ACHXOX_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR7004855.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33780..33881)
                     /gene="rhoL"
                     /locus_tag="ACHXOX_001343"
     CDS             complement(33780..33881)
                     /gene="rhoL"
                     /locus_tag="ACHXOX_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR7004856.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34023..34352)
                     /gene="trxA"
                     /locus_tag="ACHXOX_001344"
     CDS             complement(34023..34352)
                     /gene="trxA"
                     /locus_tag="ACHXOX_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR7004857.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34483..35748
                     /gene="rhlB"
                     /locus_tag="ACHXOX_001345"
     CDS             34483..35748
                     /gene="rhlB"
                     /locus_tag="ACHXOX_001345"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR7004858.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35882..37366
                     /gene="gppA"
                     /locus_tag="ACHXOX_001346"
     CDS             35882..37366
                     /gene="gppA"
                     /locus_tag="ACHXOX_001346"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR7004859.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37413..39434)
                     /gene="rep"
                     /locus_tag="ACHXOX_001347"
     CDS             complement(37413..39434)
                     /gene="rep"
                     /locus_tag="ACHXOX_001347"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR7004860.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            39651..>39790
                     /locus_tag="ACHXOX_001348"
     CDS             39651..>39790
                     /locus_tag="ACHXOX_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004861.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         9867 a        10817 c        10043 g         9063 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaagtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctgctt
     7141 tctgctttct gctttctgct ttctgctttc tgctttctat catcgcagga gaagacgtaa
     7201 aagcaagcgt ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct gcgagtataa
     7261 cgcttactcg tggggattta tgttttgctg gtagtttcgt gatcggcacc tcgcgccagg
     7321 atggctctat gcactatcgc aatgtggttt gtttgttgtc ttttttagtt ccgtcagcgt
     7381 gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga aaggcgtggt
     7441 gtaactgcgc cctaatgaag agacaaatct ttcgctacac agagacatca ttattccggt
     7501 gacggatgaa tcgtgccgga acgcagccgc agaggtgcaa actttgaagc cctgacccca
     7561 tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga cagtgaattt
     7621 catcgcctgg ctgatcaact ctggctgacc attgaagaac gcctggacga ctgggatggc
     7681 gacagcgata tcgactgcga aatcaacggc ggcgtgctga ccattacctt tgaaaacggc
     7741 agcaaaatca ttatcaaccg ccaggaaccg ctgcaccagg tatggctggc aaccaaacaa
     7801 ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag cggcgaaacc
     7861 ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt cagtttccgc
     7921 taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa aatattgctg
     7981 taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag attttgtgcg
     8041 gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact gcggcaggtt
     8101 gaagttgatg aagcttgagc cgtaggtgaa gcggtcgtgc gatgacgagt agaagcgact
     8161 gacgtcacgc accagttcct ctttgctgcc ttcgcagtgg tgatacacct caacccggtt
     8221 gctttcgtca agaatgtaga tattaaagcc attctcgtct tgcgtttctt cgaagaagaa
     8281 ctggatgatc ccttcactag caaagccgtc caccaccgcc ggtaatttga cgtgattggt
     8341 ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat aaaactcgat
     8401 ggcgttctcc agcttctgta ccgatacatt caggcgttcg aagaacaagc cccaggtttg
     8461 accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg aaaggcgcag
     8521 ttcaatacac tcagaaacca gttgctgcac gcgagtacgg attaacccgc gcagatgctg
     8581 gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca ttttgccgag
     8641 aatagttttc agggcttcga tcatcgattg ctcgccgttg aagtgcagcg tacgcacttc
     8701 gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt gattctcgcc
     8761 aaagctgaag acatccagct tacggaaatc aaaatgcacc acctgattgc ggaacgccgc
     8821 tgtcgggtca tattccaggt taacgataat cgccagatgg cggatctcac acgggctgta
     8881 gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca cgtcggcgac
     8941 catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca aacgggtgcg
     9001 cgaggtcagc agaccgttaa accatgccca cgccaccagt ttattcaggt aacggttata
     9061 ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat acagatacca
     9121 acctgaacgg ttagcccggc ccggcggcac ataaataaag gtcagattcg gttccgagag
     9181 atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt caaacgcggc
     9241 atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc taaggttatt
     9301 gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga gcaactcgtt
     9361 gtgcgcctca cgcacctgat caatcttcca gttagcacgg ttatcgagca ttgccagacg
     9421 agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac gccagcctac
     9481 acaggcgcgt tcacggctga gtttttcgca cacttttaaa tagaagcagc gacgtactaa
     9541 atccagacgg gtgaaatctt caatcgccgt caggtattca gtaacacgct ccagcatcat
     9601 gcagtatgga tcgaggccaa acgatacaat ctcgccgtcg tgcaaacgct gtttgatatc
     9661 tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca gcagtgtttt
     9721 cagcaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc tggcaccaaa
     9781 gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat ttggcgtcag
     9841 cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt cgtcgcacgg
     9901 caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat aaaattcgtc
     9961 aagcagcagt atatgctggg tggagccaca atcttcgccc cccaggctgc cgctttcatt
    10021 atgacgaaag cggttttcat caatcaggaa gaagctgact tccacaccca gcgaggcggc
    10081 ccagctttcc agcaggctgc atttacgttg tagtaactgg cgctcttcgc tatcgagcca
    10141 ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta cggacgaggt
    10201 gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggtggat cctgtactga
    10261 cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat caggcgtgta
    10321 aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg gatggtgata
    10381 gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca tagcagcaag
    10441 cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag tctcaatata
    10501 gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc gccaacatca
    10561 acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta ccacttttcg
    10621 cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca atttaacacc
    10681 ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt ttattctctg
    10741 taaattcctt attacgacag cgtgaaacgt ctgtcaggat ccactgccag acctcatttt
    10801 acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg caaggatgtt
    10861 aggatggacc acggatgata atgacggtaa caagcatgtt agacaatgtt ttaagaattg
    10921 ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac aagttgatgg
    10981 cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc gatgtgattc
    11041 ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag ctggaagtcg
    11101 cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg ccggttgaat
    11161 tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc gatgcctttg
    11221 tgtccaataa ctatgacagt ctggatgcgt taccagcagg cagtatcgtc gggacgtcca
    11281 gtttacgtcg ccagtgccaa ctggctgaac gccgcccgga tctgattatc cgctccctgc
    11341 gcggcaacgt cggcactcgc ctgagcaaac tagataacgg cgaatacgat gccatcattc
    11401 ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc gcgttaccac
    11461 ccgagatttc tcttccggcg gtaggacaag gtgcagtcgg tattgaatgc cgccttgatg
    11521 atacgcgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca ctgcgcgtta
    11581 ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca attggtagct
    11641 acgccgagct tattgatggc gaaatctggc tgcgtgcgct ggtcggcgcg ccggacggtt
    11701 cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaaa tgccaaacaa atggggattt
    11761 cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa gtctataacg
    11821 gagacgcccc ggcatgagta tccttgtcac ccgcccgtct cccgctggag aagagttagt
    11881 gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg agttttctcc
    11941 gggtcaacaa ttaccgcaac ttgctgatca actggcagcg ctgggggaga gcgatctgtt
    12001 gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctacatc agcaagatcg
    12061 taaatggccc cgactaccta attatttcgc cattggacgc accaccgcac tggcactaca
    12121 taccgtaagc ggacagaaga ttctctaccc gcaggatcgg gaaatcagcg aagtcttgct
    12181 acaattacct gaattacaaa atattgcggg caaacgtgcg ctgatattac gtggcaatgg
    12241 cggtcgtgag ctaattgggg ataccctgac ggcgcgcggt gctgaggtca ctttttgtga
    12301 atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc gctggcaatc
    12361 ccgcgaggtg acgacggtcg ttgttaccag cggtgaaatg ttgcagcaac tctggtcgct
    12421 gatcccacaa tggtatcgtg agcactggtt actacactgt cgactattgg tcgtcagtga
    12481 gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg ccgataacgc
    12541 tgccaacgat gcgcttttac gggcattaca ataactctca taacaggaag ccataatgac
    12601 ggaacaagaa aaaacctccg ccgtggttga agagactagg gaggccgtgg acaccacgtc
    12661 acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc tcagcgcggc
    12721 ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta aacaacaggc
    12781 cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat tgcaaaaagc
    12841 ccaggagagc caaaaagccg aactggaagg cattattaag caacaggcta cgcagcttga
    12901 acaggcgaat cgtcagcaag aaacgctggc aaaacagctg gatgaagtcc agcaaaaggt
    12961 cgccaccatt tccggcagcg atgctaaaac ctggctgctg gcccaggccg attttctggt
    13021 gaaactcgcc ggacggaagc tgtggagcga tcaggacgtc acgaccgctg cagcgttgct
    13081 gaaaagtgca gacgccagcc tggcggatat gaatgacccg agtctgatta ccgttcgtcg
    13141 ggcaattacc gatgatatcg ccagcctttc tgcagtatcg caggtggatt atgacggcat
    13201 catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg ccgataatga
    13261 cagcgatggt tcgccgatgg attcagacgg tgaagagctt tccagttcca tcagcgaatg
    13321 gcgtatcaat ctgcaaaaaa gctggcagaa ctttatggac aacttcatta cgattcgccg
    13381 tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc tgcgcgaaaa
    13441 tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcatcagg aagagactta
    13501 tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata ctgatgatgc
    13561 caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata tctcgatgga
    13621 tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga cccgcgtgcg
    13681 taacctgctg gcacaaccgg cagcggggac aacggaagct aaacctgcac ctgcacctgc
    13741 acctgcacct gcacctgcac ctgcacctgc accgcaaggt gatactccgg cagccgcgcc
    13801 gcaaggagaa taaccatgct aaaagtgtta ttactctttg ttttgctgat tgcggggata
    13861 gtggttggcc cgatgattgc cggtcatcag ggttacgtgc tgatccagac tgacaactac
    13921 aatatcgaaa ccagcgtcac gggcctggcg attattttga ttctggcgat ggtagtgctg
    13981 tttgccattg agtggctact gcggcggatc ttccgcacag gcgcacacac ccgtgggtgg
    14041 tttgtcggac gtaagcgtcg ccgtgcccgt aagcagaccg aacaggcgct gctgaaactg
    14101 gcggaaggcg attatcagca agttgaaaag ctgatggcga aaaatgccga tcacgcggaa
    14161 caaccggtgg tgaactatct actggctgcc gaagccgcgc aacaacgtgg tgatgaagca
    14221 cgcgccaacc aacatctgga acgcgcagca gagctggccg gcaatgacac cattccggta
    14281 gaaatcaccc gtgtacgtct gcaactggcc cgtaatgaaa accatgctgc acgccacggc
    14341 gtggataagc tgctggaagt tacgccacgc catccggaag tattacgtct ggcggaacag
    14401 gcgtatatcc gcacaggtgc atggagttct ctgctggata tcatcccatc aatggcgaaa
    14461 gcccatgttg gtgatgaaga acatcgtgca atgctggaac aacaggcatg gattggcctg
    14521 atggatcagg cgcgtgccga taacggtagt gaaggtttgc gtaactggtg gaaaaaccaa
    14581 agccggaaaa cgcgtcatca ggtagcgttg caggtggcaa tggcggaaca tcttattgaa
    14641 tgtgacgatc atgatactgc ccagcaaatt atcatcgatg gcctgaaacg ccagtacgac
    14701 gatcgcctac tgctgccgat tcctcgactg aaaaccaaca atccggaaca gctggaaaaa
    14761 gtgctgcgcc agcagatcaa aaacgtcggc gatcgcccgc tgttgtggag cacactgggc
    14821 cagtcactga tgaagcacgg agaatggcag gaagcatcgc tcgccttccg cgcagcgctg
    14881 aaacaacgtc cggacgccta cgattacgca tggcttgccg acgcgctgga cagactgcac
    14941 aagccggaag aagctgcagc gatgcgtcgc gacggtttga tgttaacgtt gcagaacaac
    15001 ccaccacagt agttccttct cacccggagg caagcacctc cgggaccttt ctgatgcata
    15061 aaaaaacgcc tgctcttatt acggagcagg cgttaaaaca ggtctgtatg acaacagtgg
    15121 gtgcttcact caacgttgtg tccatggtgt ctgatgaggc ataagcgaca tctgtcagtg
    15181 gacgataagc accgtaaacg gctctgcgtc attccggagt ttatgaggca ctaaggcgaa
    15241 cataagagat ggaatgagca tctacccgtt tattatgcca cagagaatcg ggaaataaca
    15301 tcccttaaca cttgttatga gataattctg taatcctctt tgcttcctga gtgaatattt
    15361 aacctgagct tgatcctaca catacttatt atgaatgata aaattcattc aattaataac
    15421 acatatatta attgccgtta aaactaaaaa agcattaata atcaacgcaa tataataaac
    15481 ctgccttaca tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat
    15541 tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt
    15601 cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac
    15661 cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc
    15721 cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg
    15781 ctttgccgga tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg
    15841 cacgatcatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc
    15901 cacaaaatgg ccgctttttt acttattcac caccagcaaa tgtgcctgca taatgtcact
    15961 ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg
    16021 gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag
    16081 catgaagcgg tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca
    16141 ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat
    16201 catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg
    16261 atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg
    16321 atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat
    16381 cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc
    16441 accaaattca tcggggcgca ccgaccattc cgtcagttcc ccctgaatat caccaggcgc
    16501 atgcaatttc agtccatcgc gggcagctgt ttcatcagcc agacgctcga ccaccggaat
    16561 aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg
    16621 cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag
    16681 cgtcagggca cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg
    16741 attgtggatc tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag
    16801 aaatgcacac catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt
    16861 cctgccagca ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag
    16921 taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc
    16981 gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc
    17041 gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga
    17101 accactcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac
    17161 tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag
    17221 ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc
    17281 gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc
    17341 ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta
    17401 gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
    17461 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc
    17521 taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct
    17581 ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac taccaccgca
    17641 gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc
    17701 cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat
    17761 aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc gtttggttac
    17821 ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc tttcccgtgg cgattaaggc
    17881 caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccaaca aacagcgaca
    17941 tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta
    18001 cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat
    18061 gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa
    18121 gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca
    18181 ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaact ggtacgccgt
    18241 gacgggaaac tttcgccatc gccgccggta actgacggtt tttcgccagt gcgtagagca
    18301 tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa
    18361 agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc
    18421 tgccgttgct gccaatttca ttccacggga agatggtgac gataacgaaa atcgcgccta
    18481 cgtagaaaat caggatccgc cacagcactt tgcctacagc actgcgcagc gtcacctgcg
    18541 gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca
    18601 caatacacag cgcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg
    18661 tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca
    18721 ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga
    18781 tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc accgcgatca
    18841 atgcaggtat ccactgcgcc atctccggga accagaactg gacataaacg ccaatggcgg
    18901 tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg gtgagataac
    18961 caaaaaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta acaggttcaa
    19021 ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac gaacagcccg gcgatgatat
    19081 aggccaacaa tacggatggc ccggcccatt tcagggtact ggcggccccc ataaacaggc
    19141 cgacgccaat agtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct
    19201 gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt
    19261 taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat
    19321 ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg
    19381 agtgaccact ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag
    19441 cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg
    19501 tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg
    19561 aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag
    19621 tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg
    19681 ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga
    19741 ctgccaacga tattcacatt ccattggttg cgcagtttag cttcagtttg cgccagcact
    19801 tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc
    19861 tcttcccaga gatcggcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt
    19921 actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt aattaactcc
    19981 ctgacctcgg cgttatcttc aatggtcagc attttttcag cattaatggc aaccagcgtt
    20041 ccctgcttaa gctgcccgtc agcaaacaga taatcgaggg cgtgctgcat atcacgccaa
    20101 ccaatcaact gtaagccacg cagcgtatag gtcggtgccg tggtgttgtt attcattgtt
    20161 atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt
    20221 caaaaagcca gtacagcagt tttgcgatta tcagacatgc gccgaagacc acgataaaaa
    20281 agactacgcg tgagacaaac gaatccagcc cttcacgcgc cagcacgatc atattgaaga
    20341 tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattaggc tcgcggttgc
    20401 ccagctcata cagccagtcg aaccatttga tgatcagacc aaccacgatc gccccgagcg
    20461 ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca
    20521 gtccggagtg gttattcagc acttcccagg taaagtagtt ggctgagttc agcaccatac
    20581 tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag
    20641 ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccacg
    20701 gggagaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat
    20761 tcattccata gcgttttagc gccatccaga acatgccaac aatccccagc acgcccgccg
    20821 ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga
    20881 atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg
    20941 cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc ctggcgcaga aagtagacca
    21001 ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac
    21061 tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga
    21121 agatgccgac gcttaccagc gcgataccca tcaggatcac ccacgtaaga ttggtctcca
    21181 cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg
    21241 ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca
    21301 agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga
    21361 agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca
    21421 cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacca
    21481 caaacaggcc actgaattgc agcagactca tgcgacctcc ccggcggcaa tcgccaacgc
    21541 ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt tatccaccga
    21601 cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag
    21661 caccggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt taagcacaca
    21721 aggaatgccc gcctgaatca gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat
    21781 aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc
    21841 gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc
    21901 aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc
    21961 aaattgctga tgaacggcgc gcaaggcagc aatatggtca ttgctgcggt caccggagtt
    22021 ccccaccaga atggtcattt tcccttcacg ttgccgatca ttcgccatcg tattgagaga
    22081 agggtccatc cgcgtcggga agtacagcag ttcgccccgc acctttggat gcgttttggc
    22141 aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg
    22201 acgtagcggg taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc
    22261 ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag
    22321 tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc
    22381 gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca
    22441 gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt
    22501 cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga
    22561 tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa
    22621 caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca
    22681 tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct gacttccgcc
    22741 agaatataaa accgcagtga cgctttggcg atcaccagat aaccaaagac ataagcgccc
    22801 acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta
    22861 ttcgacaaca gcaaccagat agcaaaatca cgcagcaacc agacggtaaa actcgccgcc
    22921 gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt
    22981 tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc
    23041 aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac
    23101 tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc
    23161 aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc
    23221 agatagctta acgggatgac accacgtttg atcaacatga tggcggcagg aatcaccacc
    23281 agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg
    23341 acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg
    23401 gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc
    23461 accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacag tccctggctg
    23521 attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc
    23581 gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg
    23641 tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac ggtaatcaac
    23701 tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc cagcaactta
    23761 ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct
    23821 tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt
    23881 gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt
    23941 gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg
    24001 aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg
    24061 gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg
    24121 aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat cgtagtagtt
    24181 ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag
    24241 gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt
    24301 atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc
    24361 tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata
    24421 gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc
    24481 tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat tatgcttttt
    24541 cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat
    24601 aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc
    24661 cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat tggcggtgga
    24721 gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc
    24781 cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg
    24841 ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct
    24901 acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg
    24961 aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta
    25021 tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac
    25081 cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctgca ccgcgtccag
    25141 ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca taaccgcgaa
    25201 taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg cgcacggcat
    25261 tttcaatcca ctgtgcataa aagcgtccgc tggcgtcagg cgcataccac ggcgcacgaa
    25321 aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata tcggtctctt
    25381 gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa tcaacttcac
    25441 cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta tttgatgccg
    25501 caattttggc ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt
    25561 cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg
    25621 gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt
    25681 ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag
    25741 gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaag
    25801 gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg
    25861 agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc
    25921 agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag
    25981 aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttccagcgag
    26041 atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc catcacctga
    26101 tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag
    26161 ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc gttgaggaag
    26221 gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc
    26281 agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc
    26341 ggcgtagtga tgatgagaat ttcgcggata tcggccaaca tcagcaccga cagcggatag
    26401 taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga
    26461 tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc ctccgctgaa
    26521 aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac ctgcttccac
    26581 cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc aaaggtttcc
    26641 tgtggcagcc agccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca
    26701 tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc
    26761 ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta
    26821 cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa
    26881 tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca
    26941 tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc
    27001 ggaaagtggt aaggaccgta gttattcgag cagttggtga taagcgtcgg cagaccgtag
    27061 gtccgcagcc aggcgcgcac cagatggtcg ctgctggctt ttgacgcgga ataagggctg
    27121 ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat
    27181 acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta
    27241 ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata
    27301 aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca
    27361 cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact
    27421 ttctcaaagg caaagcgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg
    27481 taggtcagct tatcgaccac taccaccgcg tcgctcgttt cgttgatgat ataacgcacc
    27541 agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga
    27601 ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata actttaaact
    27661 gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg
    27721 ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca
    27781 gagtttcgcc gctatgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc
    27841 gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg
    27901 cgcgtttatc ggtagccgcc aggcaatcag ccaccgccgc tttcacctga tcgataaccc
    27961 agaacggttt gtgatcgttc acttcgcgcg cggtacggat aagccgcgcc tgctgggggt
    28021 tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag
    28081 gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa
    28141 tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga
    28201 agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac
    28261 cttcgaggaa aattttgtac agttcgctgg cgcgggtcga acaaaccggc gtcataccac
    28321 caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac acgcgttccg
    28381 ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg
    28441 gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt
    28501 caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa
    28561 cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca atcagccagg
    28621 catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta
    28681 ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg
    28741 cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg
    28801 taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat
    28861 ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt
    28921 atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc
    28981 gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg
    29041 acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg
    29101 aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa
    29161 atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg
    29221 ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt
    29281 ggcaatgtct gccaacgcgt ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct
    29341 gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat ttgccgccag
    29401 ttctgaacgc agcgtatcgc tgctcatcac ctggtcacgc acccataaca gtgcatcaat
    29461 aactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg
    29521 ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt
    29581 agcctcttcc ggccacggcg aataaagatc gcccgtgcgc agaccagcct caacgtgacc
    29641 aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgtcg ttgtgtcgcc
    29701 gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat
    29761 ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg
    29821 tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg cagtgacgca
    29881 aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggcg ccatcttgat
    29941 ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc
    30001 ggcgagcgcc tttgcgctca ccgcagcagt gttgctattt cgagcaacgg cgggttaatg
    30061 cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac
    30121 gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac
    30181 gcggatccag ggttggacca acattcaggg tgtttaacat ggcccgattc tgatcatagt
    30241 cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac
    30301 gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc
    30361 gcgaaatatt atgctgctca gcaattttca gcgcctgttc aatactgttc atccggcggt
    30421 cgtagatggc tttcgccacc tcttcctgac gcttcacctg tgctttcatc tggatggtac
    30481 gcgccgccca tgcgcctttc agctcatcat tcagatggct ggctgcacgc tggctggcaa
    30541 aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcagca ataagcttca
    30601 cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg ttaatcattt
    30661 catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgtttg taatagtcgg
    30721 tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat
    30781 aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg acatccaggt
    30841 tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg
    30901 taatcgccgt cgagctccac tcctgacgag caaaaaaagt atacgccagc gcgattaacg
    30961 caaatgccag ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc
    31021 cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca
    31081 tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg
    31141 ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag
    31201 gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc
    31261 tgccagcacg ccaatggaag cgagcagtgc cgcagcaagg gtaatcagca caaacgcctg
    31321 acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa
    31381 tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat
    31441 ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc
    31501 gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac
    31561 tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat
    31621 catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat
    31681 tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt
    31741 gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag
    31801 caccatctcc caggagccaa agatataacc caggctactg agataaagtt tgccgaacac
    31861 catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa
    31921 acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc
    31981 atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat
    32041 ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa
    32101 accgactttt tttgccacct tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat
    32161 actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta
    32221 ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccgaacccac
    32281 cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacacgcc agcaaattcc
    32341 aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa gcaaaacgcc
    32401 acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag
    32461 aaatcgtcat tggtcttggt catggccagt ttattaatga ggaattccat tgcatcgatt
    32521 tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagta
    32581 gtgagcagtt cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt
    32641 ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg
    32701 tagataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatagtcagg
    32761 ctgccgccct cttccacgtt acgcgccgca ccaaagaagc gtttcggacg atgcagggcg
    32821 ttggcatcca caccaccggt caacacttta cctgacgccg gaacaacggt gttgtaagcg
    32881 cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg
    32941 cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca
    33001 aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga
    33061 cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctgg
    33121 gcaatgttct gcagcagcat ggttttaccg gctttcggcg gtgccacaat cagaccacgc
    33181 tgaccacgac cgataggtga tgccagatcc agtacgcgag cagttaaatc ttcagtagaa
    33241 ccgttaccac gttccatacg cagacgagaa tttgcgtgca gcggggttaa gttctcaaag
    33301 aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc
    33361 gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg
    33421 cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg
    33481 taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca
    33541 tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc
    33601 tgcttacgca tgcgagccag gttttccagc cccatatttt cgccgagagt gatcagctca
    33661 gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg
    33721 gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt
    33781 catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa
    33841 gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc
    33901 gttggaaatt acaagattca aatcttaata aggtatgttt aatacgaagt caacactaag
    33961 ttagcatgac tcacgccggg cgtccagttt ttagcaacgg ggcacccgaa catgaaattc
    34021 ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcgcccact
    34081 ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca
    34141 tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc
    34201 tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac
    34261 tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg
    34321 tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat aagcctggcg
    34381 tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat ttcaccggat
    34441 acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa aacacattta
    34501 acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa
    34561 aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac gctggcgggt
    34621 cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca
    34681 acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa tcaaccgcgt
    34741 gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccatgccga cgcagaaccg
    34801 ctggcggaag ctactggcct gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa
    34861 cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg tcgtttaatt
    34921 gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt tctggatgaa
    34981 gccgatcgca tgtacgatct gggctttatt aaagatatcc gctggctgtt ccgccgtatg
    35041 ccgcctgcaa accagcgcct caacatgctg ttctccgcca cgctttcgta ccgggtacgt
    35101 gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc ggaacagaaa
    35161 acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa aatgcgtttg
    35221 ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc caataccaaa
    35281 caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt cggtttgttg
    35341 acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac ccgtggcgat
    35401 ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg
    35461 cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg tattggtcgt
    35521 acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga gtatgcattg
    35581 aatttgcctg ctattgaaac ctatattggt cactcaattc cggtaagcaa atacaatccg
    35641 gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg tacaggcaat
    35701 ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat
    35761 actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat gagcatactg
    35821 tagtaagtga ttcaggtgaa agaatgcagc caacacagag acagattgaa ggatgaagag
    35881 tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata gttttcatat
    35941 gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa aacgcaaagt
    36001 gcgtctggct gctggcctga atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg
    36061 ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct cgcaaattcg
    36121 cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta ttgccaatgc
    36181 gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag cacgtctgat
    36241 ttatcagggc gttgctcaca caactggcgg tgccgatcag cgcctggtgg tggatatagg
    36301 cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt tgttcagcct
    36361 gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc tggggcagga
    36421 aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt
    36481 acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg cgttacagga
    36541 aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc agcaattgaa
    36601 acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc tgacgctgga
    36661 acgtgcgtta gtgttcccga gtggtctggc gatcctgatc gccattttta ccgaactgaa
    36721 tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt
    36781 gcatctggcc gtcgagcagg atattcgcag ccgtacgctg cgtaatattc agcgccgctt
    36841 tatgatcgat attgatcagg cgcagcgcgt agccaaagtt gcggctaact tcttcgatca
    36901 ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca
    36961 gcttcatgaa atcggcctga gcgttgactt taaacaagcg ccgcaacacg ctgcttatct
    37021 ggtgcgtaat ctggatcttc ccggttttac ccccgcacag aaaaaattgc tggcgacgct
    37081 actgctcaac cagactaatc cggtcgatct ctcatcgctg catcagcaaa atgccgtacc
    37141 gccgcgcgtc gcagaacaac tctgccgttt actccgcctg gccatcattt ttgccagccg
    37201 tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac tgttgacctt
    37261 gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta ttgctcagga
    37321 aagccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag taagtgccgg
    37381 atgcgatgct gacgcatctt ttccggcctt gattatttcc ctcgttttgc cgccatcatc
    37441 gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc gctgaccact
    37501 ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc
    37561 tccgggcgca ccagctcgcc gtactgacgg cgttctttac acagcgtaaa ggtcaattcc
    37621 ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat attatcttca
    37681 tcgatgctgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta gacataagga
    37741 aactccagcc cttttgacgc gtgcagagtc atcagttgca cctgatccag ctcttcttca
    37801 ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc
    37861 atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa cagttggttg
    37921 acgttcttca tgcgcatttc ggcggctttc gggctgggcg atgtttcgta cagccaggat
    37981 tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg ctccgccaga
    38041 cgctggattt ctgccaacca gtgagtaaag cgggtcaatg cttcataacc acgtccgcta
    38101 agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc
    38161 gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg cgtgttaacg
    38221 atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata agccagcaag
    38281 tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta cgggatgcgg
    38341 ttttgcatca ggaatttttc aaacactcgc gactgatggt taccgcgata aagaatagcg
    38401 taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc agtgacgcgc
    38461 tcggcttcat gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc gtaacccagt
    38521 tcggagaaca gacgcttctc aaagacgtgc gggttattgg cgatcaggat gttcgccgct
    38581 ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt cagcgccgga
    38641 aaatcctgac tcagcagcac caggttttgt ggacgtgcgc cgcgccagga gtagatcgac
    38701 tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt
    38761 tcatactggc tggtgttggt gtcctgatac tcatccacca gcagatagcg aattttgttc
    38821 tgccagcgct cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga
    38881 tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat catacagccc ataacaatgg
    38941 gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg tgttttgaga
    39001 tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt gtcatcttca
    39061 atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc aaacagcgaa
    39121 aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag ccccagcgtg
    39181 tggaaggttg agatcatcag cccacgcgcc tctttgcgac ccagcgtctg tccgacacgc
    39241 tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg tcgcgcctga
    39301 taaccgcaac cgcggatcag atgggcgatt ttattggtga tcacacgagt tttaccggaa
    39361 cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg ttgttggccg
    39421 gggtttagac gcataggtgt attgctcaat cttcgaacgg ggggaggatt gtagcagaat
    39481 cgcatttgaa tgtgaccgcg tgtaagccgc taaccgattg gctgcggatg tttaaatcag
    39541 agagggaaga ccgaaaccgg agttcatcgt gaatccatta gacttagaaa atatcgggtc
    39601 gtaacgttgt gttattactt tttcagagtt tactaaggag aggtgtactc gtggcgatta
    39661 atttcagtcc taaggttggt gaaatactgg attgcaactt tggtaactat cctgttagcc
    39721 agaatggaca gttcagcacc acgtattacg atggccgcat tcctcctgaa atgataaaaa
    39781 acaggttagt
//
LOCUS       DAZRVY010000025        39409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZRVY010000025 DAZRVY010000000
VERSION     DAZRVY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.025"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHXOX_001349"
     CDS             <1..266
                     /locus_tag="ACHXOX_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR7004862.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHXOX_001350"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHXOX_001350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR7004863.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXOX_001351"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXOX_001351"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR7004864.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHXOX_001352"
     CDS             complement(1570..3270)
                     /locus_tag="ACHXOX_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR7004865.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHXOX_001353"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHXOX_001353"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR7004866.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHXOX_001354"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHXOX_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHXOX_001355"
     CDS             4828..6600
                     /locus_tag="ACHXOX_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR7004867.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHXOX_001356"
     CDS             6593..8374
                     /locus_tag="ACHXOX_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR7004868.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXOX_001357"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXOX_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR7004869.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXOX_001358"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXOX_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR7004870.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXOX_001359"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXOX_001359"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR7004871.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHXOX_001360"
     CDS             11336..11908
                     /locus_tag="ACHXOX_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR7004872.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHXOX_001361"
     CDS             complement(11939..12250)
                     /locus_tag="ACHXOX_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR7004873.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHXOX_001362"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHXOX_001362"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHXOX_001363"
     CDS             12629..12982
                     /locus_tag="ACHXOX_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR7004874.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXOX_001364"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXOX_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHXOX_001365"
     CDS             complement(14736..15206)
                     /locus_tag="ACHXOX_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR7004875.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXOX_001366"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXOX_001366"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR7004876.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHXOX_001367"
     CDS             complement(16044..16262)
                     /locus_tag="ACHXOX_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR7004877.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHXOX_001368"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHXOX_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR7004878.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHXOX_001369"
     CDS             complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHXOX_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR7004879.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHXOX_001370"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHXOX_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR7004880.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="ACHXOX_001371"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="ACHXOX_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR7004881.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="ACHXOX_001372"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="ACHXOX_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR7004882.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25913..26011
                     /locus_tag="ACHXOX_001373"
                     /pseudo
     CDS             25913..26011
                     /locus_tag="ACHXOX_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHXOX_001374"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHXOX_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR7004883.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHXOX_001375"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHXOX_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR7004884.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="ACHXOX_001376"
     CDS             26836..27213
                     /locus_tag="ACHXOX_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR7004885.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="ACHXOX_001377"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="ACHXOX_001377"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR7004886.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHXOX_001378"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHXOX_001378"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR7004887.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="ACHXOX_001378"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="ACHXOX_001379"
     CDS             30024..30353
                     /locus_tag="ACHXOX_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR7004888.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="ACHXOX_001380"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="ACHXOX_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR7004889.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="ACHXOX_001381"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="ACHXOX_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR7004890.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="ACHXOX_001382"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="ACHXOX_001382"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR7004891.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="ACHXOX_001383"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="ACHXOX_001383"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR7004892.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHXOX_001384"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHXOX_001384"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR7004893.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="ACHXOX_001385"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="ACHXOX_001385"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR7004894.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHXOX_001386"
     CDS             complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHXOX_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR7004895.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39320..>39409)
                     /locus_tag="ACHXOX_001387"
     CDS             complement(39320..>39409)
                     /locus_tag="ACHXOX_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004896.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9527 a        10050 c        10419 g         9413 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgata cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAZRVY010000026        38338 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZRVY010000026 DAZRVY010000000
VERSION     DAZRVY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.026"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            60..1424
                     /locus_tag="ACHXOX_001388"
     CDS             60..1424
                     /locus_tag="ACHXOX_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7004897.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1552..2043)
                     /gene="yajQ"
                     /locus_tag="ACHXOX_001389"
     CDS             complement(1552..2043)
                     /gene="yajQ"
                     /locus_tag="ACHXOX_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR7004898.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2211..3122
                     /gene="panE"
                     /locus_tag="ACHXOX_001390"
     CDS             2211..3122
                     /gene="panE"
                     /locus_tag="ACHXOX_001390"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR7004899.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3085..3675
                     /gene="yajL"
                     /locus_tag="ACHXOX_001391"
     CDS             3085..3675
                     /gene="yajL"
                     /locus_tag="ACHXOX_001391"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR7004900.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3729..5177)
                     /gene="thiI"
                     /locus_tag="ACHXOX_001392"
     CDS             complement(3729..5177)
                     /gene="thiI"
                     /locus_tag="ACHXOX_001392"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR7004901.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5383..5625
                     /gene="xseB"
                     /locus_tag="ACHXOX_001393"
     CDS             5383..5625
                     /gene="xseB"
                     /locus_tag="ACHXOX_001393"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR7004902.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5625..6524
                     /gene="ispA"
                     /locus_tag="ACHXOX_001394"
     CDS             5625..6524
                     /gene="ispA"
                     /locus_tag="ACHXOX_001394"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR7004903.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6549..8411
                     /gene="dxs"
                     /locus_tag="ACHXOX_001395"
     CDS             6549..8411
                     /gene="dxs"
                     /locus_tag="ACHXOX_001395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR7004904.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8466..9440
                     /gene="yajO"
                     /locus_tag="ACHXOX_001396"
     CDS             8466..9440
                     /gene="yajO"
                     /locus_tag="ACHXOX_001396"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR7004905.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9494..10012)
                     /gene="pgpA"
                     /locus_tag="ACHXOX_001397"
     CDS             complement(9494..10012)
                     /gene="pgpA"
                     /locus_tag="ACHXOX_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR7004906.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9990..10967)
                     /gene="thiL"
                     /locus_tag="ACHXOX_001398"
     CDS             complement(9990..10967)
                     /gene="thiL"
                     /locus_tag="ACHXOX_001398"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR7004907.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11045..11464)
                     /gene="nusB"
                     /locus_tag="ACHXOX_001399"
     CDS             complement(11045..11464)
                     /gene="nusB"
                     /locus_tag="ACHXOX_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR7004908.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11484..11954)
                     /gene="ribE"
                     /locus_tag="ACHXOX_001400"
     CDS             complement(11484..11954)
                     /gene="ribE"
                     /locus_tag="ACHXOX_001400"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR7004909.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12043..13146)
                     /gene="ribD"
                     /locus_tag="ACHXOX_001401"
     CDS             complement(12043..13146)
                     /gene="ribD"
                     /locus_tag="ACHXOX_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR7004910.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13150..13599)
                     /gene="nrdR"
                     /locus_tag="ACHXOX_001402"
     CDS             complement(13150..13599)
                     /gene="nrdR"
                     /locus_tag="ACHXOX_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR7004911.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13750..14289
                     /locus_tag="ACHXOX_001403"
     CDS             13750..14289
                     /locus_tag="ACHXOX_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR7004912.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14588..15472
                     /gene="tsx"
                     /locus_tag="ACHXOX_001404"
     CDS             14588..15472
                     /gene="tsx"
                     /locus_tag="ACHXOX_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR7004913.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15649..15996)
                     /gene="yajD"
                     /locus_tag="ACHXOX_001405"
     CDS             complement(15649..15996)
                     /gene="yajD"
                     /locus_tag="ACHXOX_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR7004914.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16125..17096)
                     /gene="secF"
                     /locus_tag="ACHXOX_001406"
     CDS             complement(16125..17096)
                     /gene="secF"
                     /locus_tag="ACHXOX_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR7004915.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17107..18954)
                     /gene="secD"
                     /locus_tag="ACHXOX_001407"
     CDS             complement(17107..18954)
                     /gene="secD"
                     /locus_tag="ACHXOX_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR7004916.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18982..19314)
                     /gene="yajC"
                     /locus_tag="ACHXOX_001408"
     CDS             complement(18982..19314)
                     /gene="yajC"
                     /locus_tag="ACHXOX_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR7004917.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19337..20464)
                     /gene="tgt"
                     /locus_tag="ACHXOX_001409"
     CDS             complement(19337..20464)
                     /gene="tgt"
                     /locus_tag="ACHXOX_001409"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR7004918.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20520..21590)
                     /gene="queA"
                     /locus_tag="ACHXOX_001410"
     CDS             complement(20520..21590)
                     /gene="queA"
                     /locus_tag="ACHXOX_001410"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR7004919.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21683..22264
                     /gene="acpH"
                     /locus_tag="ACHXOX_001411"
     CDS             21683..22264
                     /gene="acpH"
                     /locus_tag="ACHXOX_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR7004920.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22269..24086)
                     /gene="malZ"
                     /locus_tag="ACHXOX_001412"
     CDS             complement(22269..24086)
                     /gene="malZ"
                     /locus_tag="ACHXOX_001412"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR7004921.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24242..25615)
                     /gene="proY"
                     /locus_tag="ACHXOX_001413"
     CDS             complement(24242..25615)
                     /gene="proY"
                     /locus_tag="ACHXOX_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR7004922.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25691..27010)
                     /gene="brnQ"
                     /locus_tag="ACHXOX_001414"
     CDS             complement(25691..27010)
                     /gene="brnQ"
                     /locus_tag="ACHXOX_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR7004923.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27417..28712)
                     /gene="phoR"
                     /locus_tag="ACHXOX_001415"
     CDS             complement(27417..28712)
                     /gene="phoR"
                     /locus_tag="ACHXOX_001415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR7004924.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28770..29459)
                     /gene="phoB"
                     /locus_tag="ACHXOX_001416"
     CDS             complement(28770..29459)
                     /gene="phoB"
                     /locus_tag="ACHXOX_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR7004925.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29649..30851
                     /gene="sbcD"
                     /locus_tag="ACHXOX_001417"
     CDS             29649..30851
                     /gene="sbcD"
                     /locus_tag="ACHXOX_001417"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR7004926.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30848..33991
                     /gene="sbcC"
                     /locus_tag="ACHXOX_001418"
     CDS             30848..33991
                     /gene="sbcC"
                     /locus_tag="ACHXOX_001418"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR7004927.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34117..35301
                     /gene="araJ"
                     /locus_tag="ACHXOX_001419"
     CDS             34117..35301
                     /gene="araJ"
                     /locus_tag="ACHXOX_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR7004928.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35444..36352)
                     /gene="mak"
                     /locus_tag="ACHXOX_001420"
     CDS             complement(35444..36352)
                     /gene="mak"
                     /locus_tag="ACHXOX_001420"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR7004929.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36477..37388
                     /gene="rdgC"
                     /locus_tag="ACHXOX_001421"
     CDS             36477..37388
                     /gene="rdgC"
                     /locus_tag="ACHXOX_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR7004930.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37466..37921)
                     /locus_tag="ACHXOX_001422"
     CDS             complement(37466..37921)
                     /locus_tag="ACHXOX_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7004931.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37918..38253)
                     /locus_tag="ACHXOX_001423"
                     /pseudo
     CDS             complement(37918..38253)
                     /locus_tag="ACHXOX_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9465 a        10314 c         9734 g         8825 t
ORIGIN      
        1 ctgtttaccc aacatgcgct ccgcactaca ctatgtgggc atttttttaa ggggtggtaa
       61 tgaacgatta taaaatgacg ccaggtgaga ggcgcgcgac ctggggttta gggaccgtat
      121 tctcgttgcg catgctgggc atgtttatgg ttctgccggt tctgaccacg tacggcatgg
      181 ctctgcaagg tgccagcgaa gcattaatcg gtattgccat tggtatttat ggtctgactc
      241 aggccgtttt tcagattccg tttggcctgc tttccgaccg cattggtcgc aaaccattaa
      301 ttgtcggtgg gctggcggtg tttgccgccg gtagcgttat cgctgcgctt tccgactcca
      361 tctggggaat tattctgggc cgggcgctac aaggctctgg agcaattgcc gccgccgtta
      421 tggcgctgct ttccgatctc acgcgcgaac aaaaccgcac caaagcgatg gcgtttatcg
      481 gcgtgagctt tggcattacc tttgccattg cgatggtgct tggcccgatc atcactcaca
      541 aacttgggct gcacgcgctg ttctggatga tcgctattct ggcaacaacc ggcattgcgt
      601 tgaccatttg ggttgtgccc aacagtagca ctcacgtact taatcgtgag tccggaatgg
      661 tgaaaggcag tttcagtaaa gtgctggcgg aaccgcggct gctgaaactc aactttggca
      721 ttatgtgtct gcacatgctt ctgatgtcta cgtttgttgc cctgcccgga cagctagctg
      781 atgcggggtt cccggcggct gaacactgga aggtctatct ggcgacgatg ttaatcgcct
      841 ttggctcgat cgtgcctttc attatctacg ctgaagttaa gcgcaaaatg aagcaagtct
      901 ttgtcttctg cgtcgggttg atcgtggttg cggaaattgt gttgtggaac gcacaaacgc
      961 agttctggca actggtggtc ggcgtgcagc ttttctttgt ggcgtttaat ttgatggaag
     1021 cccttctgcc ctcacttatc agtaaagagt cgccagcagg ttacaaaggt acggcgatgg
     1081 gtgtctactc caccagccag tttcttggcg tggcgattgg cggttcgctg ggcggctgga
     1141 ttgacggcat gtttgacggt cagggggtat ttctcgctgg cgcaatgctg gccgcagtgt
     1201 ggctggcagt cgccagtacc atgaaagaac cgccgtatgt cagcagtttg cgcattgaaa
     1261 tcccggcgga cattgccgca aacgaagcgt taaaagtgcg tttgctggaa actgaaggcg
     1321 tcaaagaagt gttgattgca gaagaagagc attcagctta tgtgaaaatc gacagcaaag
     1381 tgacaaatcg ctttgaggtt gaacaggcaa ttcgtcaggc ataaaaaacg cggcgaattg
     1441 cctgctgcgc tacgcttacc aggcctacaa agttcttgag ttacaaaccg ttgtaggccg
     1501 aatcaggcgt tcatgccgca tctgacatga acaaaacgca cattgtcgcg attaatcgcg
     1561 gaagtttttg aactggaacg gctggccgag atcgccacca cgtaccatcg ccatgacagc
     1621 ctgcaaatca tcacgagatt tgccagtcac acggatctca tcgccctgaa tttgcgcctg
     1681 cactttcagt ttgctgtctt taatcatctt gacgattttc ttctgggtcg cgctctcaat
     1741 gccctgtttc agtttcgctt ccacaaacca ggttttaccg ctatgaacga tattttccgg
     1801 tacatccagt gaactgcctt caatgccgcg cttcagcagc ttggcacgca gaatatccag
     1861 caactgattg acctggaaat cggactcgct caacactttg atggttttgc tggcgtcgtt
     1921 cagctcaaat gaggcttcaa cattacggaa gtcaaaacgg gactccactt cgcggctcgc
     1981 gttatcgacc gcgttacgtg cttcctgaag atcaacttca gagacaatat cgaaagatgg
     2041 catcttttcc tctcccttca tttttgatgc gaagcataat acccgcaaag ttaaataacc
     2101 gacagtcctg caccaggacg tatcccttaa ttgtgcatag tcacaacagc atgcacaatt
     2161 aagggatagc cttattgaac acccggagtg gttgcgggtg aggaggaaca atgaaaatta
     2221 ccgtattggg atgcggtgcc ttagggcaat tatggcttac agcactttgt aaacagggtc
     2281 acgaagttca gggctggctg cgcgtaccgc aaccttattg tagtgtgaat ctggttgaga
     2341 ctgatggttc gatatttaac gaatcgctga ccgccaacga tcccgatttt cttgccacca
     2401 gcgatctgct cctggtgacg ctgaaagcat ggcaggtttc cgatgccgtc aaaagcctcg
     2461 cgtccacact gcctgtaacc acgccaatac tgttaattca caacggcatg ggcaccatcg
     2521 aagagttgca aaacattcag cagccattac tgatgggcac caccacccat gccgcccgcc
     2581 gcgacggcaa tgtcattatt catgtggcaa acggaatcac gcatattggt ccggcacggc
     2641 aacaggacgg cgattacagc tatctggcgg atattttgca aaccgtgttg cctgacgttg
     2701 cctggcataa caatattcgc gccgagctgt ggcgcaagct ggccgtcaac tgtgtgatta
     2761 atccactgac cgccatctgg aattgcccga atggtgaatt acgtcatcat ccgcaagaaa
     2821 ttatgcagat atgcgaagaa gtcgcggcag tgatcgagcg cgaagggcat catacttcag
     2881 cagaagattt gcgtgattac gtgatgcagg tgattgatgc cacagcggaa aatatctcgt
     2941 cgatgttgca ggatatccgc gcgctgcgcc acaccgaaat cgactatatc aatggttttc
     3001 tcttacgccg cgcccgcgcg catgggattg ccgtaccgga aaacacccgc ctgtttgaaa
     3061 tggtaaaaag aaaggagagt gaatatgagc gcatcggcac tggtttgcct cgcccctggt
     3121 agtgaagaga ctgaagccgt caccactatc gatctgctgg ttcgcggcgg tatcaaagtc
     3181 accactgcca gcgtcgccag cgatggcaac ctggccatta cctgctcgcg cggcgtgaag
     3241 ctgctggcgg atgcgccgct ggtcgaggtg gctgatggcg aatatgacgt gatcgtactg
     3301 cctggtggca ttaaaggcgc agagtgtttt cgcgatagta ccctgctggt tgaaaccgtt
     3361 aaacagttcc accgttccgg gcgtatcgtc gcggctattt gcgccgcgcc agccaccgtg
     3421 ctggtaccgc acgatatctt cccgattggc aatatgaccg gcttcccgac gctgaaagac
     3481 aaaattcccg ccgaacaatg gcaggacaag cgcgtcgtct gggatgcacg ggtaaaattg
     3541 ctgaccagcc aggggccggg tacggctatt gattttggtc tgaaaattat cgacctgctg
     3601 gttgggcgcg agaaagccca tgaagtggca tcacaactgg tgatggcggc agggatttat
     3661 aattattacg agtagtgtcg gatgcggcaa acgtcgcatc tgaccagatg cgacgtaaaa
     3721 accacaaatt atgggcgata caccttcaca ttgttaaagc cctgctcgcg cagatagagc
     3781 gcctgcagac ggctcatcac cccgcgctca caccacagta gccaggttct gttctggtcg
     3841 agatcgccaa atttggtgct cagtttatag aacggcagag aaaccacgtc gataccttca
     3901 accttcagcg gcttatcttc ctgctcatcg acagaacgga tatcgaggat cacgtcgttc
     3961 gggccaaagc cattgacggt ttccacttcc accacttcct gctcggtctg ctgggcgatt
     4021 tcgcggatat caacgttatt cgcttcctca accactttat cgagaatact gaagtcgaac
     4081 ttctcttctt ccgcttcaat cttcgattta accgctttca ccgtcgggct tttggagatc
     4141 acgccacaat attctggcat cgtgcgagca aagtcttcag tgccaatctg gcgggccagg
     4201 ttgatgatgt gctctttgtc gtaagaaatc agcggacgca ggatcagcgt atcagagacg
     4261 ttatcaatca ggcgcaggtt ggtcagcgtc tgactggaca cctggccgag cgcttcgccg
     4321 gtgaccagcg cctgtacgcc gtaacgttca gccactttag acgcggcacg caccatcata
     4381 cgtttgagga taacgcccat ctgaccgtcg tcgattttct cgagaatttc cccgacgacc
     4441 ggttcgaaat taatggcgac aaaacgcacg cggtgggagc tgccaaagcg gttccacaga
     4501 taatgcgcca cctgacgcac gccaatttca tgcgctgcgc cgccgaggtt aaagaagcag
     4561 taatgcacgc ggcagccgcg acgcatcaac atataactgg aaacaccgga gtcgaaacca
     4621 ccggaaatga gcgacagcac atcttcctgg gtgccaatcg ggaaaccgcc aataccttcg
     4681 tagcggcctt taatcagcag aagacgatcg tcttccactt ccagatggac agttacatcc
     4741 gggttagtca gcttcacgcg cgcggattca atatgctgat ttaaaccgcc gccgacgtaa
     4801 cgttccacat caatcgagct aaaatcatgt ttgccacggc gcttcacgcg tacgcagaag
     4861 gttttgcctt ccagctgatc gcgatattga accaacgctt tctcgaaaat atcgtgcatg
     4921 tcggtaaacg gcacgtcttc gacttcgaga atatggtgga tacccggaat acgggtcagg
     4981 gcgtcgcgaa tagccagacg ctgtttttca tcttttgcgc gaacttcgat gttatcccag
     5041 tggcggacga cagcgagcgt ctcatcatag tgctttaaaa cgttacgaat gttcccggta
     5101 aggattttta taaagcgcaa gcgcacagat tggcttttga tggtgatttc cgggaacaat
     5161 ttaatgataa acttcatggc ggcaatggtt cgttggcaag ccttaagcga cttgtatagg
     5221 gaaaaataca gcagcccaca cctgcggctg catccaggcg cggaagtata ccactaacat
     5281 cgctttgctg cgcacatcac cttaccattg cgcgttattt gctatttgcc ctgagtccgt
     5341 taccatgacg gggcgaaaaa tattgagagt cagacattca ttatgccgaa gaaaaatgag
     5401 gcgcccgcca gctttgaaaa ggcgctgagc gagctggaac agattgtaac ccgtctggaa
     5461 agtggcgacc tgccgctgga agaggcgctg aacgagttcg aacgcggcgt gcagctggca
     5521 cgtcaggggc aggccaaatt acaacaagcc gaacagcgcg tacaaattct gctgtctgac
     5581 aatgaagacg cctctctacc cccttttaca ccggacaatg agtaatggac tttccgcagc
     5641 aacttgaagc ctgcgttaag caggccaacc aggcgctgag ccgttttatc gccccactgc
     5701 cctttcagaa cactcccgtg gtcgaaacca tgcagtatgg cgcattatta ggtggtaagc
     5761 gcctgcgacc ttttctggtt tatgccaccg gtcatatgtt cggcgttagc acaaacacgc
     5821 tggacgcacc cgctgccgcc gttgagtgta tccacgctta ctcattaatt catgatgatt
     5881 taccggcgat ggatgatgac gatctgcgtc gcggtttgcc gacctgccat gtgaagtttg
     5941 gcgaagcaaa cgcgattctc gctggcgacg ctttacaaac gctggcgttc tcgattttaa
     6001 gcgatgccga tatgccggaa gtgtcggacc gcgacagaat ttcgatgatt tcagaactgg
     6061 caagcgccag cggtattgcc ggaatgtgcg gtggtcaggc attagattta gatgcggaag
     6121 gcaaacacgt acctctggac gcactcgagc gtattcatcg tcataaaacc ggcgcattga
     6181 ttcgcgccgc cgttcgcctt ggtgcattaa gcgccggaga taaagggcgt cgtgctctgc
     6241 cagtactcga caagtacgca gagagcatcg gccttgcctt ccaggttcag gatgacatcc
     6301 tggatgtggt gggagatact gcaacgttgg gaaaacgcca gggtgccgac cagcaacttg
     6361 gtaaaagtac ctaccctgca cttctgggtc ttgagcaagc ccggaagaaa gcccgggatc
     6421 tgatcgacga tgcccgccag tcgctgaaac aactggctga acaatcactc gatacctcgg
     6481 cactggaagc gctagcggac tacatcatcc agcgtaataa ataaacaata agtattaata
     6541 ggcccctgat gagttttgat attgccaaat acccgaccct ggcactggtc gactccaccc
     6601 aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct
     6661 atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg
     6721 aactgaccgt ggcgctgcac tatgtctaca acaccccgtt tgaccaattg atttgggatg
     6781 tggggcatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa atcggcacca
     6841 tccgtcagaa aggcggtctg catccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat
     6901 taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg gttactgccg
     6961 agaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg attaccgctg
     7021 gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg ctggtggtcc
     7081 tcaacgacaa tgaaatgtcg atttccgaaa atgtcggcgc gctcaataac catctggcac
     7141 agctgctttc cggtaagctt tactcttcgc tgcgcgaagg cgggaaaaaa gttttctctg
     7201 gcgttccgcc aattaaagag ctgctcaaac gtaccgaaga acatattaaa ggcatggtag
     7261 tgcctggcac gttgtttgaa gagctgggct ttaactacat cggcccggtt gacggtcacg
     7321 atgtgctggg gcttatcacc acgctgaaga acatgcgcga cctgaaaggc ccgcagttcc
     7381 tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac cccatcactt
     7441 tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt agcggcggtt
     7501 tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg aaagacaaca
     7561 agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag ttttcacgta
     7621 aattcccgga tcgttacttc gacgtggcaa tcgccgagca acacgcggtg acctttgccg
     7681 ccggtctggc gattggtggg tacaaaccca ttgtcgcgat ttactccact ttcctgcaac
     7741 gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gctcccggtc ctgttcgcca
     7801 tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca tcagggcgct tttgacctct
     7861 cttacctgcg ctgcataccg gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc
     7921 gccagatgct ctataccggc tatcactata acgacggccc gtccgcggtg cgctacccgc
     7981 gcggtaacgc ggttggcgtg gaactgacgc cgctggaaaa actgccaatt ggcaaaggca
     8041 ttgtgaagcg tcgtggcgag aaactggcga tccttaactt tggtacgctg atgccagacg
     8101 cggcgaaagt cgctgaatcg ctgaacgcta cgctggtcga tatgcgtttt gtgaaaccgc
     8161 ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc accgtagaag
     8221 aaaacgccat tatgggcggc gcaggcagcg gcgtgaacga agtgctaatg gcccatcgta
     8281 aaccagtacc cgtgctgaac attggcctgc ctgacttctt tattccacaa ggaactcagg
     8341 aagaaatgcg cgccgaactc ggcctcgatg ccgccggtat ggaagccaaa atcaaggcct
     8401 ggctggcata atccctactc cactcctgct atgcttaaga aattattcat ggcaggagtg
     8461 gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga ctttgcctcg
     8521 gctgtatgac ctttggcgag ccagatcgtg gtaatcacgc atggacactg ccggaagaga
     8581 gtagccgtcc catcattaaa cgcgcgctgg aaggcggcat aaatttcttt gataccgcca
     8641 atagctattc cgatggcagc agcgaagaga tcgttggtcg cgcactgcgg gatttcgccc
     8701 gtcgtgaaga cgtggtcgtt gcgacgaaag tgttccatcg cgttggtgat ttaccggaag
     8761 gattatcccg tgcacaaatt ttgcgctcta tcgacgacag cctgcgccgt ctcggcatgg
     8821 attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc gaagagacgc
     8881 tggaagccct gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc gcgtcatcca
     8941 tgcacgcttc gcagtttgct caggccctga agctacaaaa acagcacggc tgggcgcagt
     9001 ttatcagtat gcaggatcac tacaatctga tttatcgcga agaagagcgc gagatgctgc
     9061 ctctgtgtta tcaggagggc gtggcggtga ttccgtggag cccgctggcg cgggggcgac
     9121 tgacgcgtcc gtggggagaa actaccgcac gactggtgtc tgatgaggtg gggaaaaatc
     9181 tctataaaga aagcgatgaa aatgacgcgc agatcgcaga gcggttaacg ggcgtcagtg
     9241 aagaactcgg tgcaacacga gcacaagttg cgctggcctg gttattgagt aaaccgggca
     9301 ttgccgcacc gattatcggt acatcgcggg aagaacagct tgatgagctg ctgaacgcgg
     9361 tggatatcac tttgaagcca gaacagattg ccgaactgga aacgccgtat aaaccgcatg
     9421 cggtcgtagg atttaaataa gagtccaggc ctgatgagac gtgacaagcg tcacatcagg
     9481 catcggtgca caactacgac agaataccca gcggccagtg atgaccgata aagtacagga
     9541 tgcctgcgga aatcaccccg gcgacaatat cgtcgatcat gatccccatg ccgccatgta
     9601 cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata cggaaaatca
     9661 caaacccggc ggcaacccac tgccagtcat tggtcggcag cgccatgagc gtgatccaca
     9721 taccaataaa ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata tctttcgccg
     9781 tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc gagtagagct
     9841 gccagggtaa aaaggtcatc agataccaga acggaatcgc tgccagcgat cccatcgtgc
     9901 caggaacgat tgggcttaat ccacttccga atccgacagc aagcagatgc cacggattac
     9961 tcatcttcag gcgacttttc gcgacatctt tatggcgtgg caaaatggtc atatcctttc
    10021 cagtcaaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat atcggcggtc
    10081 atttgcccga tacaggtaaa cggtacgcca aggtgtccga gagcaacatc cagcgcgcca
    10141 cggttcagtt ccggcaccgt gaaacacaac tcgtaatctt caccgccaga gagcgcccag
    10201 cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg cagcaatgct
    10261 aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc ggaaatcaga
    10321 ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata
    10381 cgcggcgatg gacggagatg acgtttgatc aagtagtcag catctttagc atcggcaacc
    10441 tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt accggtcaca
    10501 tagatccagt cacccggttt cgccccagag cgcgttaagg ctcgtcccat cggaacaaag
    10561 ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc
    10621 atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg
    10681 tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc catcgctgcc
    10741 agatcgctta ggttcaccgc cagtgcttta taagccagat cagcaggatc gatatcaggg
    10801 aggaaatggt tacccgccac cagcgtatca gtgctgatcg ccagggtctg tttctcgggg
    10861 atattgagaa gtgcgcaatc gtcgccgatg cccagttcga catcaagacg agaacttctt
    10921 acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg ttatgcctca
    10981 gcaggtaaaa aagaaaaggc cgacgatcat ggaacggtct tccgtgaatc taccggcctg
    11041 gatatcactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg ccgttgacga
    11101 acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca
    11161 ctttgtatgg cacatcgcta cgtttagaca gttcgtacag cgcaatgcgc agtactgctt
    11221 tttctacctg tcccagttct tccagcaggc gggacaggta tggcttcatc agtccgtcga
    11281 ggtatgcggt attagtcgcc accccggcca gcagctcacg gaagtacagg acgtcaacgt
    11341 cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg ttctgggaca
    11401 actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga gcagcaggtt
    11461 tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt aatcatttca
    11521 agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc acgttcgatc
    11581 gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg
    11641 gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaagtg ggcagtgcca
    11701 ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt agccagtgca
    11761 cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat gttttcatct
    11821 tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct gtcattgata
    11881 aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc aacgttagct
    11941 tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg ggcggatttt
    12001 agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac ccactaaatg
    12061 caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg gggcgtcggc
    12121 taatttctca agccctggca gcgtgcataa tccgcgggcg tcgctgccta atagtttagg
    12181 tgcgatatag acaatcagct catcgactaa acccgcctgc agcaatgcgc cagcgagcgt
    12241 tggccccgct tccacccaga tgctgttaat ttgctgttta cccagttgca tcatcagtac
    12301 aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg tttccggcca
    12361 ctcactagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg
    12421 atgttccggc gtcacgcgat tttggctatc aatcacaata cgtactggct ggcggagatt
    12481 ttgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca ctgttaaggc
    12541 tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt gtgcgcgctg
    12601 tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc cgctcgccat
    12661 cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa agccggtgcg
    12721 catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca gcccgtggct
    12781 gacgtcaatg ccagcctgtt gcagacggta aagtccacgc ccagcgacct gcgggttagg
    12841 atcctgcatc gctgcgacca cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg
    12901 tggcgtacgg ccatgatggc tacagggttc gagtgtcaca tacgccgttg cgcctttggc
    12961 tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg
    13021 gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat tcgggttggg
    13081 gtgggtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc
    13141 ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa
    13201 actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt tcaattgctc
    13261 catcaccaga ttgccaatca tcttgctcgg tacttcgcgc tcaccggtgg cgcgcagctg
    13321 cgatttaata tgattgattg ccatttcaac gtcatcggaa ctcaccggac gtttttccag
    13381 cgcccgcagc attccgctac gcaatttctc ttcattaaac ggttcacgca cgtcgttgct
    13441 ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac gttcattaca
    13501 caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac gagagtcaat
    13561 taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg accaggtggt
    13621 taacggaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg cttttgagat
    13681 aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc gacgagaaag
    13741 ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct gttcagcctt
    13801 agcgcctgtg tgcagcaaag tgaagtgcga cagatgaaac acagcgtcag cacgctgaac
    13861 caggagatga cgcaactcaa tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat
    13921 gcaaaatcca gcagtggggt ttaccttctg cctggagcga aaacaccggc aagactggaa
    13981 agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatac agatggcacc
    14041 acccttacac tacgtattca gggtgagtcg aatgatccct tgcccgcatt cagcggaact
    14101 gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa tgtgcagaat
    14161 caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat tccgttacaa
    14221 ttaaagggga tttctgtgga tcagttaggc tttgtacgta tccacgacat tcaacctgtt
    14281 atgcagtaaa cgtatttcgg gacgattttg tgcgtcccgc aacatctttc cccgtcattt
    14341 tgttactctg cttacatcac ctggattgat agtaaaagtt tgcaacaagg gcgaaagtca
    14401 gtacaatccc cgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa
    14461 ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc tctgtatacg
    14521 aaatatttag aaacgcaatt tgcgcctttt tcactcccgc aagggatttt caaacagtgg
    14581 catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct cttcgtcttt
    14641 tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt ggcaccagag
    14701 cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagatccgca acgataccta
    14761 ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc
    14821 gccggtattc ttcggcggta actccgatgc taaaggtatc tggaaccacg gttctccgct
    14881 gtttatggaa atcgaaccac gtttctccat cgacaagctg accaatactg accttagctt
    14941 cggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg gtcgtaataa
    15001 agatggtcgc cagagcacct ggtacatggg tctgggtacc gacatcgata ctggtctgcc
    15061 gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactatg acgcagcgaa
    15121 cgaaaacgag tgggacggtt accgtttcaa aattaaatac tttgtgccga ttaccgatct
    15181 gtggggcggt cagctgagct acatcggctt caccaacttc gactggggtt ccgatttagg
    15241 ggatgacagc ggtaacgcaa tcaacggtat taagacccgt actaataact ctatcgcttc
    15301 cagccatatt ctggctctga actacgatca ctggcactac tctgtcgtag ctcgttactg
    15361 gcacgacggt ggtcagtgga acgacgatgc agaactgaac ttcggcaacg gcaacttcaa
    15421 cgttcgctct accggctggg gtggttacct ggtagtaggt tacaacttct gattatgaaa
    15481 atgccgggat ttattcccgg catttctgat tgttaacaac gtgtgctttg ttcatgccgg
    15541 atgcggcgta aacgctttgt ccggcccaca aaagcacgta aattcaatat attgctgaaa
    15601 taacgtaggc ctgataagcg tagcgcatca ggcaatttta cgttttaatc acttcttctt
    15661 gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga catctttctg
    15721 cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat atttcgaatg
    15781 ctcatgatcg tggcaataga gacaaagcaa ttcccagtta ctgccatctt ccgggttatt
    15841 ggtatggtcg tgatcaatgt ggtgaacggt aagttcacgc aggttggaat aaacaaactc
    15901 gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc gatagccgct
    15961 ttctaaccgc gcatagtttt ttgggatgat agccatgaaa agttacctgg atgaaatgta
    16021 gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca tcaaaaaaaa
    16081 tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattacgg cagaattgac
    16141 ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg cttcataccc
    16201 agtttcagag ccaacgcaga tgctacgtag atggaagatg cagtgccgat ggaaacaccg
    16261 ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagagata cagcatcagg
    16321 ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac
    16381 acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc acgaattcgg
    16441 tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa cgatgccaca
    16501 atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat gataacgtcg
    16561 tgcgccagcg caataaccac ccctgccgcc agtcgccact caaagcggaa acctacgtac
    16621 acgaggatag acagcagcgc tgccatcaac gccatcgcac cggtttgcgc aaggtctgcc
    16681 cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt ggtggattcg
    16741 ttaatcacct tcagaacctg gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc
    16801 ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg ctcttcaaaa
    16861 ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc gagcgtaatt
    16921 tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac gcccataata
    16981 acgatagcag cgattaacag cagaccagag atgccgaaag cccagtagtc ccagcgcata
    17041 aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg tgccacatcg
    17101 cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt ttacgatggc
    17161 acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg tagtaatcgc
    17221 gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa tcagcgtggt
    17281 gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa ttgcctgttg
    17341 aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta cgttcgcatc
    17401 gaccgctacc gcaagggtta agacgatacc cgcgatacct ggcatactca gtgttgcgcc
    17461 tggcaacagc gacataatac cgacgatcaa gatcaagttg gcaatcagag cactggtcgc
    17521 aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca gcagaccggc
    17581 aaggcaagcc tccagcccct gctcaatgtt ctgcataccc agggtcgggc caattgtacg
    17641 ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg
    17701 acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca gacgagactg
    17761 gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac catttgcatc
    17821 tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc cgatgttgtc
    17881 cttggtgaag ttagacataa tgttgccacc agcgctatcg agcgagatgt taacctgcgg
    17941 ctggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg tcagaattac
    18001 gcgtttgtac agcacaaccg gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg
    18061 tacgcgaccg gatgccgcag cggcctgatc aacattggtg tttaccagac ggaattccag
    18121 cgttgccgtc gcgcccagaa tctctttcgc acgcgcagtg tcctgaatac ctggcagttc
    18181 aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc caagttggtt
    18241 tacacggtta cgcaggatat taatgttctg ctgcaccgca tattcacgcg cttcactcag
    18301 acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctgctaa tcaccaggtc
    18361 cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat cgcggaaagt
    18421 gatgctcagg ccgtagttgt tttctttacg aacagtggta tacgggatgc ccttttcgcg
    18481 caggtcactg cgtaggctat cgatattttg ttcctggagt ttgccaagcg cggtgtccat
    18541 atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct tcatcggctc
    18601 agcatgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg ccacgacgta
    18661 tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc aactgggtgt cagtggagtc
    18721 gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta ttttttcttc
    18781 ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc
    18841 agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca gcagaccgat
    18901 gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta acacggcaat
    18961 tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca ggacggcagc
    19021 tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag caatgtagcc
    19081 gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa cttcatcacc
    19141 tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct gtggacgcag
    19201 gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag acatcgggct
    19261 accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt
    19321 tccctcatta ttaatattaa tcaacgttca aaggtggtac ttctcgcccc tgacgctggt
    19381 aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt aaacccgcca
    19441 tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt
    19501 tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac
    19561 actcaggatc aagtgggcca gtatcgctct tatacttcgc attgcggatt ttcaccacgc
    19621 catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg cagtcaaaca
    19681 tatcgatacc acgacgtacg ccttcaacca ggtcttctgg tttaccaacg cccatcaggt
    19741 aacgcggttt gtctgccgga atttgcgggc atacatgctc cagaatgcgg tgcatatctg
    19801 ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaaaccg atatctacca
    19861 gacctttaac agaaatatca cgtaaatctt cgtaaacgct gccctgaatg ataccaaaca
    19921 gcgcattttt gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc caacgcagag
    19981 acatctccat ggagcgtttt gcgtaatccc agtcagcagg atacggcgta cactcatcaa
    20041 agatcatgac gatatccgaa ccaagatcgt actgaatctc cattgatttt tcaggatcga
    20101 ggaaaatcgg atcgccgttg atcgggttac ggaagtgcac gccctgttcg gtgattttac
    20161 gaatatcgcc aaggctgaag acctggaagc cgccggagtc ggtgaggatc ggccccttcc
    20221 actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc
    20281 acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact tcttccggcg
    20341 tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt tccactacgc
    20401 cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt
    20461 tcattttttc tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc gccacggaat
    20521 tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg
    20581 taactaaaaa agcgatattt ctcttctacc gctgctttat aggcgttcat ggtgtgttga
    20641 taaccggcaa aggccgaaac cagcataatc agcgtcgatt ccggcaggtg gaagtttgtc
    20701 accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg ggtatcgtca
    20761 aagaacggtt cgataagatc gtttttcgcc gcctgagccg cgctttccag cgaacgtact
    20821 gaggtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct
    20881 accacatcct gcggcacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg
    20941 acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc catctccacg
    21001 cctttggcgc gtaatttttc cagcaaaggt tcgtcaaaat gcagacctgc ggtcggggct
    21061 gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc agcgtcttcg
    21121 tccggacggt cgatatacgg cggcagcggc atatggccga tgctgttgag aatatccagc
    21181 accgagcgtt catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt
    21241 gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg cgctttcgag
    21301 gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gttcaaccag cacttcaatc
    21361 ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt attattaaaa
    21421 accagaagat cgccggggtt gagcttatca agtaaatcgg tgaaagtacc gtgcgtcagc
    21481 gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catgggatag
    21541 tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat gacactgact
    21601 cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca acctttaccc
    21661 ccttttggat agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc
    21721 ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag
    21781 ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg tattaaccga
    21841 caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaactc gccgcgttgc
    21901 gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt cgcagctgtc
    21961 gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga tgacgatttt
    22021 gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag agcagtggct
    22081 ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg caagccgccg
    22141 cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt atgacgccct
    22201 cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac acaaggcgtt
    22261 ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag aatcccgtca
    22321 gtcagttgcc catgcctttc tttgcattgc cattgcacgg cattgagcaa cggtgacgcg
    22381 ggtagcacga cttcacaagc ttcgccacgg ttgattgcca ccagtacacg ctgctgattc
    22441 agcacgcgga caaataccac cacgttatct tccgcataca gcacctgaca gccgccatga
    22501 cgtagcgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc gaataacgcc
    22561 gtatcctgct tttccacctg ccaggggaac ggtttacggc aaaacggatc gtttttgcca
    22621 tccagtccta cttcatcacc gtaataaatg cacggtacac caggccaggt gaacagccag
    22681 accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta
    22741 tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg
    22801 taattatcca tccaggccat acaggtttgg gcatcaattt gctgcggatc gtaagagata
    22861 tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt catggcggca
    22921 tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc gacaatatac
    22981 gcttccggct gggtttcttt cgccgcttcg gtgctcccag caacgtgctg catattattg
    23041 cgcgccccac ccgcctcccc tagcatatgc accacatcca gccgccagcc gtccatactc
    23101 cacggcgctt tcagccaatg gcggacaata ctgtcttccc cgcgataaat ttcattcacc
    23161 agactttccg actgataatc cagcttcggc aagctggcat agccaagcca gtcgagcgcc
    23221 gtgccatcat cactaaacga gtaccagtcg cgccagggcg attcagggtt gtgacaagct
    23281 ccccccgtgc cacgattgtg cctgtcaaac caggcatggg aatcgccact gtggttaaac
    23341 acgccatcca gccccagccg cattcccagc tgctgcgtat tgtggcgtaa gcgcagcaac
    23401 gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc gtatttatgt
    23461 acgctgggag ctttaaacac cggattgaga tacagcgccg tcacgccaag ctttttcaga
    23521 tacggcagtt tttcgcttat cccgtccaga tcgccgccat agaacgttga tccgcccgcc
    23581 tgcgccgtga ccggttcatc ccagtcacgc aagatgatct cttgtccggc tgcatgatgg
    23641 taatagacat gatcctgttc agcttcacga ggaagactac gcgcaaaacg atcagggaag
    23701 atctgataaa aaatctgatc cgcagcccat tgtgggccga tatccggtac atcgacggca
    23761 aactgctcca gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg
    23821 cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg gttgtccgct ggagagatca
    23881 atcgccgcac gccatgcggt gacgccaggc tgctgctgac tgcgatgctt atgcatcgat
    23941 actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg
    24001 cccgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa tgggggcacc
    24061 ggcaggtgcc atgcatttaa catcataatt cccctgtgat gaaaatctga tcaccttgcc
    24121 atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag gacgggggcg
    24181 gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca gaacgcaagg
    24241 attactggtt ttcagccagc tgacgatcgt ggcgacgctt aaacatccag ccaatcaaca
    24301 gcacaacaat ccacgcgaaa ccgacataca gcgagatacg cgtatccggg tgataaccaa
    24361 tcaacccgat aataaagagc aggaaaatca gcccgccgat ggtcgttgct accccacccg
    24421 gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acagcgacgg aaggcaattt
    24481 gcgacagcag gatcataatc cacacccaca ccgtggcgaa ggttgccagc gaagcgatca
    24541 ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc agcgcggtag
    24601 tcatcaccag caccgtaacc cacggaatac cgcgacgcga cgttttgctg aaaattttcg
    24661 gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacgccaaag acgtcactgt
    24721 taattgccga tagcgaagcg gtcagcacaa caaagttaag aatgctggcg gcaaaggtaa
    24781 tgcccatatg ctggaacgtc agcacgaacg ggctaccggc agtgccaacc tgattccacg
    24841 ggtagataga cataatgacg aacagcgtac cgacgtagaa caccagaata cgcatcggca
    24901 cggagttaat cgcacgcggt atcgatttct caggatcttt cgcttcaccg gcggtaatcc
    24961 cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatc accatcccaa
    25021 gccagccgtt actgaagaag ccgccgttgc tccacagatt atgaataccg gtcggttgcc
    25081 cgccgttgcc aatcccccag atgatgatgc cgaaaccagc gacaatcatg atgatgatgg
    25141 tggcgacttt aaagaacgag aaccagaatt ccagctcccc gaagaccttc acgctcatca
    25201 ggtttacggc gcaaatgatc agcaccacgc tcagtaccca aatccagtgc ggcaccgtcg
    25261 ggaaccagac acccatatag ataccaaaag cggtcacatc ggcgatggcg acaataagga
    25321 tttcaaagca gtaggtccag ccggtaatgt aacctgccag cgggccgagg ttttcctgcg
    25381 cataacgcga gaaagagctg gcggccgggt tatgtaccga catttccccc agcgcacgca
    25441 taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctc ggaccggcca
    25501 ttttgatggc gtctgccgaa ccgtaaaaca gcccggtacc aattgctgaa cccagtgcca
    25561 taaagcgtat gtggcgggta cttagcccac gctttagctt gttcttactt tccatcaatt
    25621 cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt taaaacaaat
    25681 gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga tcccagataa
    25741 tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt tcggccagcg
    25801 gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc agatgctttg atcccgtcga
    25861 gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac gaattatgcc
    25921 accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa atagcggtca
    25981 gcaccggtac ggagatctgg atcagctggc ttaagccgag gttagaaacc accatcgaga
    26041 agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg gcaaagaatt
    26101 ctgcacaagc acaggtcagg ccaactgccg ttaccaagca ggcgatgaag attaacgccg
    26161 ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc agaatagcag
    26221 cgccgtttgc agactgatcg accagcgacg cgctgtctga acccagacgg aacagcgcca
    26281 ggtacagcag agtcagacca acacccgcca tcaggccagc ccagacggta taacgggtca
    26341 gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg ataccaaaca
    26401 ccattgcgcc cagcgtatcc atggtcagat agccgttaac gaagccgtta gaaaacgcag
    26461 cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagaca atcgcggcaa
    26521 cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc acggtatcca
    26581 gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc aggctgtaga
    26641 taaacagcgg cagcgcggaa tcaccagtca gcggcgcaat ccccacttca aaggaaacgg
    26701 tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc gttgccagca
    26761 gcacgccagc gactttaccg attggcgtgc tgaggctgtc aacaccgccg ccaacttttg
    26821 ccagcgccac taccgttaat accggcaggc caacggcagt aatgaggaag ccgaatgccg
    26881 cagtccagac gtgttcgcca gcctgcaagc cgaccattgg agggaaaata atgttacctg
    26941 cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc gatcttaatt
    27001 gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca
    27061 atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata ctcccaacca
    27121 ggcgcggcga agaatagttt ttcgatttac cgcgttaatg cagtcagtat gacagcacct
    27181 gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga gggatctaaa aaccagaggt
    27241 atatagcgat aagaaatcac caaccagtat aaaacactat ttccgtgaaa aagacatggc
    27301 taatcatgcg aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta ttttgactgc
    27361 aaagaaaata agccagcatc gactggctta tggcaataaa agatgacaaa ggcggattaa
    27421 tcgctgtttt tggcaattaa acgttccggg ataacaaaac tgaaacgtgt tccttttcct
    27481 actgtactct caatattcag gcgactttcg tgatgattca cagcatgttt cacgatcgct
    27541 aaccctaatc cgctaccgcc ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc
    27601 tcggtcaggc gcggaatatg ctccggtgca atgcccggtc cgttatcttc aacgctaaat
    27661 tcggcaccgt gcggcactcg ctgccagcgt acggtgatat gcgtgccttc tggagtatga
    27721 ttcacggcgt tatagaccag gttcgaaatc gcactgcgta gctgatcttc gttgccagac
    27781 actttgaggc cgttatctat ctcaaaggta aatgtctgtt ttttctgact cagagtctga
    27841 gcctcgcgct caacaacgcg cagcatcatc ggcacatcaa ccttttcatt gagcaattgc
    27901 gtcggcgcgg cttcaatttt cgacagcgtt agcaactgct tcaccagccc ttccatccgc
    27961 tgggtctgct cgcgcatggt gtgcaacgct ttttcgcgta ccgcgccttc cagcggctgc
    28021 tcatccatca tctccaggta accctgtaac acggtcaatg gcgtacgtaa ctcatggctc
    28081 acgttggcaa aaaagttacg ccgcgcccct tccagttgat gcatttgcgt gacatcacgc
    28141 gccaccatca gcaactgttt gtgggtataa ggcatgacgc gaatttccag atgccgcccg
    28201 gtgttgagca ccagattgag cgggcgagaa aaatcacgcg ttttcagata ttgcgtaaac
    28261 tccgggtaac gcagtaggtt aaggatgttc tgcccgttat cttccggcca gcgcaaacca
    28321 agaatttgtt gcgccagacc gttacaccag aaaataccgc tctcttccgt ggtcagcacc
    28381 accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt gcccagttca
    28441 cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc cgtatagtag cggttcccag
    28501 ctaccacgcc ccggtggcgg ggtcatactg cgatccaccc acagccacca tgaaaggcgc
    28561 aataaattcc agaaatgcca gataagcagt cctgttaccg atgccagcaa aaaccagggc
    28621 aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc agcaaagtag cagctccagc
    28681 accagccttt tccacgacag ccgttccagc acgtaagata ctcccgttaa gaaatcataa
    28741 gccctgctct gcgtccgatg agcaaggcgt taaaagcggg ttgaaaaacg atatcctgta
    28801 ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt acgcaggcga
    28861 cgaatgtgga catcgaccgt gcggtcttcc acatacacgt tagttcccca gacgtggttt
    28921 aacagctgct cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg cagcagttta
    28981 aattctgtcg gccccatctc cagcggctct tcgcccgcca tcactcggtg agatgtcggg
    29041 tcgagactta atccctgcat ctcaatcacc tcttccaccg ccattggcga aatacggcgc
    29101 attaccgctt tgattcgcgc caccagctcc ttcggcgaaa acggcttggt gatatagtca
    29161 tccgcgccgg tttcaaggcc gcgcacgcga tcttcttctt cccctctggc ggtcaacatc
    29221 accactggaa tatcccgggt catcgactcg cgcttaaggt gtttgatgaa ctggataccg
    29281 gagccgccag gtaacatcca gtcaaggaga attaaatccg gccagggttc attcagttga
    29341 ttcacagcac tgtcataatc ttccgcttcg accggctgaa agccattttg ttcgagcacg
    29401 aagcagacca tttcgcgaat tggagcttca tcttctacga ccagaatacg tctcgccatg
    29461 atttgccctg ttgtaataaa taggttgcga tcattaatgc gacgtcatta tgcgtcagat
    29521 ttatgacaga tttatgaaaa gctcgtcgca catatcttca ggttattgat ttccgtggcg
    29581 cagaaaaaag caaatggcac atctgtttgg gtataatcgc gcccatgctt tttcgccagg
    29641 gaaccgttat gcgcatcctt cacacctcag actggcatct cggccagaac ttctacagta
    29701 aaagccgcga agctgaacat caggcttttc ttgactggct gctggagaca gcacaaaccc
    29761 atcaggtgga tgcgattatt gttgccggtg atgttttcga taccggctcg ccgcccagtt
    29821 acgcccgcac gttatacaac cgttttgttg tcaatttaca gcaaactggc tgtcatctgg
    29881 tggtactggc aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg
    29941 cgttcctcaa tactaccgtg gtcgccagcg ccggacatgc gccgcaaatc ttgcctcgtc
    30001 gcgacgggac gccaggcgca gtgctgtgcc ccattccgtt tttacgtccg cgtgacatta
    30061 ttaccagcca ggcggggctt aacggtattg aaaaacagca gcatttactg gcagcgatta
    30121 ccgattatta ccaacaacac tatgccgatg cctgcaaact gcgcggcgat cagcctctgc
    30181 ccatcatcgc cacgggacat ttaacgaccg tgggggccag taaaagtgac gccgtgcgtg
    30241 acatttatat tggcacgctg gacgcgtttc cggcacaaaa ctttccacca gccgactaca
    30301 tcgcgctcgg gcatattcac cgcgcacaga ttattggcgg catggaacat gttcgctatt
    30361 gcggctcccc cattccactg agttttgatg aatgcggtaa gagtaaatat gtccatctgg
    30421 tgacattttc aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg gtaacgcaac
    30481 ccatggcagt gctgaaaggc gatctggcgt cgattaccgc acagctggaa cagtggcgcg
    30541 atgtatcgca ggagccacct gtctggctgg atatcgaaat cactactgat gagtatctgc
    30601 atgatattca gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg
    30661 tacgtcggag tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca
    30721 gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc actggaagaa ctggatgaat
    30781 cgcagcagca acgtctgcag catcttttca ccacgacgtt gcataccctc gccggagaac
    30841 acgaagcatg aaaattctca gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg
    30901 gaagattgat ttcacccgcg agccgttcgc cagcaacggg ctgtttgcta ttaccggccc
    30961 aacaggtgcg gggaaaacca ccctgctgga cgccatttgt ctggcgctgt atcacgaaac
    31021 tccgcgtctc tctaacgttt cacaatcgca aaatgatctc atgacccgcg ataccgccga
    31081 atgtctggcg gaggtggagt ttgaagtgaa aggtgaagcg taccgtgcat tctggagcca
    31141 gaatcgggcg cgtaaccaac ccgacggtaa tttgcaggtg ccacgcgtag agctggcgcg
    31201 ctgcgccgac ggcaaaattc tcgccgacaa agtgaaagat aagctggaac tgacagcgac
    31261 gttaaccggg ctggattacg ggcgcttcac tcgttcgatg ctgctttcgc aggggcaatt
    31321 tgctgccttc ctgaatgcca aacccaaaga acgcgcggaa ttgctcgagg agttaaccgg
    31381 cactgaaatc tacgggcaaa tctcggcgat ggtttttgag cagcacaaat cggcccgcac
    31441 agagctggag aagctgcaag cgcaggccag cggcgtcacg ttgctcacgc cggaacaagt
    31501 gcaatcgctg acagcgagtt tgcaggtact tactgacgaa gaaaaacagt taattaccgc
    31561 gcagcagcaa gaacaacaat cgctaaactg gttaacgcgt caggacgaat tgcagcaaga
    31621 agccagccgc cgtcagcagg ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc
    31681 tcaactggcg gcgcttagtc tggcacaacc ggcacgaaat cttcgtccac actgggaacg
    31741 catcgcagaa cacagcgcgg cgctggcgca tattcgccag cagattgaag aagtaaatac
    31801 tcgcttacag agcacaatgg cgcttcgcgc gagcattcgc caccacgcgg cgaagcagtc
    31861 agcagaatta cagcagcagc aacaaagcct gaatacctgg ttacaggaac acgaccgctt
    31921 ccgtcagtgg aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac aaaccagcga
    31981 tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa aacttaatgc
    32041 gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt gctagcgccc tggcgcaaca
    32101 ttccgagcaa cgcccactgc gtcagcgcct ggtcgcgctg catgggcaga ttgttcccca
    32161 acaaaaacgt ctggcgcagt taatggtcac tatccagaat gtcactctgg aacagacgca
    32221 acgtaatgtc gcacttaacg aaatgcgcca gcgttataaa gaaaagacgc agcaacttgc
    32281 cgatgtgaaa accatttgcg agcaggaagc gcgcatcaaa acgctggaag ctcaacgtgc
    32341 acagttacag gcgggtcaac cttgtccact ttgtggttcc accagccacc cggcggtcga
    32401 ggcgtatcag gcgctggagc ctggtgttaa tcagtctcga ttactgacgc tggaaaacga
    32461 agttaaaaag ctcggcgaag aaggtgcggc gctgcgcggg caactggatg cattaacgaa
    32521 gcagcttcag cgcgatgaaa acgaagcgca aagcctccga caagatgagc aagcacttac
    32581 tcaacaatgg caagccgtca cgaccagcct caatatcacc ttgcagccac aggacgatat
    32641 tcaaccgtgg ctggatgcac aagatgagca cgaacgccag ctgcggttac tcagccaacg
    32701 acatgaatta caagggcaga ttgccgcgca taatcagcaa attatccagt atcaacagca
    32761 aattgaacaa cgccagcagc agctcttaac ggcattgacg ggttatgcac tgacattgcc
    32821 acaggaagat gaagaagaga gctggttggc gacacgtcag caagaagcgc agagctggca
    32881 gcaacgccag aacgaattaa ccgcgctgca aaaccgtatt cagcagctga cgccgattct
    32941 ggaaacgttg ccgcaaagtg atgatctccc gcacagcgaa gaaactgtgg cgctggataa
    33001 ctggcggcag gttcatgaac aatgtctcgc attacacagc cagcagcaga cgttacagca
    33061 acaggatgtt ctggcggcgc aaagtctgca aaaagcccag gcgcagtttg acaccgcgct
    33121 acaggccagc gtctttgacg atcagcaggc gttccttgcg gcgctaatgg atgaacaaac
    33181 actaacgcag ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac
    33241 gctggtcaca cagacagcag aaacgctggc acagcatcaa caacaccgac ctgacgggtt
    33301 ggctctcact gtgacggtgg agcagattca gcaagagtta gcgcaaactc accaaaagtt
    33361 gcgtgaaaac accacgagtc aaggcgagat tcgccagcag ctgaagcagg atgcagataa
    33421 ccgtcagcaa caacaaacct tactgcagca aattgctcaa atgacgcagc aggttgagga
    33481 ctggggatat ctgaattcgc taataggttc caaagagggc gataaattcc gcaagtttgc
    33541 ccaggggctg acgctggata atttagtcca tctcgctaat cagcaactta cccggctgca
    33601 cgggcgctat ctgttacagc gcaaagccag cgaggcgctg gaagtcgagg ttgttgatac
    33661 ctggcaggca gatgcagtac gcgatacccg taccctttcc ggcggcgaaa gtttcctcgt
    33721 tagtctggcg ctggcgctgg cgctttcgga tctggtcagc cataaaacac gtattgactc
    33781 gctgttcctt gatgaaggtt ttggcacgct ggatagcgaa acgctggata ccgcccttga
    33841 tgcgctggat gccctgaacg ccagtggcaa aaccatcggt gtgattagcc acgttgaagc
    33901 gatgaaagag cgtattcctg tgcagatcaa agtgaagaag atcaacggcc tgggctacag
    33961 caaactggaa agtacgtttg cagtgaaata actattcagc aggataatga atacagaggg
    34021 gcgaattatc tcttggcctt gctggtcgtt atcctgcaag ctatcacttt attggctacg
    34081 gtgattggta gccgttctgg tggttgtgat ggtggtatga aaaaagtcat tttatctttg
    34141 gctctgggca cgtttggttt ggggatggcc gaatttggca ttatgggcgt gctcacggag
    34201 ctggcgcata acgtaggaat ttcgattcct gctgccgggc atatgatctc gtattatgca
    34261 ctgggggtgg tggtcggtgc gccaatcatc gcactctttt ccagccgcta ctcactcaaa
    34321 catatcttgt tgtttctggt ggcgttgtgc gtcattggca acgccatgtt cacgctctct
    34381 tcgtcttacc tgatgcttgc cattggtcgc ctggtttccg gctttccgca tggcgcattt
    34441 tttggcgtcg gcgcgatcgt gttatcaaaa attatcaaac ccggaaaagt caccgccgcc
    34501 gtggcgggga tggtttccgg gatgacagtc gccaatttgc tgggcattcc gctgggaacg
    34561 tatttaagtc aggaatttag ctggcgttac acctttttat tgatcgctgt ttttaatatt
    34621 gcggtgatgg catcggtcta tttttgggtg ccagatattc gcgacgaggc gaaaggaaat
    34681 ctgcgcgaac aatttcactt tttgcgcagc ccggccccgt ggttaatttt cgccgccacg
    34741 atgtttggca acgcaggtgt ctttgcctgg ttcagctacg taaagccata catgatgttt
    34801 atttccgttt tttcggaaac ggcgatgacc tttattatga tgttagttgg gctagggatg
    34861 gtgctgggaa atatgctaag tggcaggatt tcaggacgtt attcaccact gcgcattgca
    34921 gcagtgactg actttatcat tgtactggca ctgctgatgc tctttttctg cggcggcatg
    34981 aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg gattatttgc cctttcagca
    35041 cctctgcaaa tattgttact gcaaaacgcc aaaggcggag agttattagg tgccgcaggt
    35101 gggcaaatag cgtttaacct cggtagcgcc gtcggcgcat attgcggagg tatgatgctg
    35161 acgctggggc tggcatataa ttacgtggcg ctgcctgccg ccctgctttc gtttgctgcg
    35221 atgtcatcgt tgctgctgta tggtcgctat aagcgccagc aagcggcgga tagtccggtg
    35281 ctggcgaaac cactggggta gattactgtc acggtgcgcg ttgcttattg ccggatgcgg
    35341 cgtgaacgcc ttatccgccc tacgcggttc tggcacattt tgtaggcctg ataagacgcg
    35401 gcaagcgtcg catcaggcat gatgcgccaa ttgcctacgt tttttactct tgtggccata
    35461 accacgcagc gccgcgtacg ccgctggaat caccgtgctt cgccttacgc accggcgttt
    35521 cacattcgcc gccgaagaca aattgtttaa tcaactgccc aaccgtttga tataaacggt
    35581 ctacattgct catcccgccc cccaggacaa tcacatccgg atcgagaata ttcacgacat
    35641 gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa tgccagttcc gctaccggat
    35701 cgctttcttc aaccaggcgg ataatttcac tgcctttcag cgcatgtccg ctcaaacgac
    35761 gataatccgt cgcgaatccc gtgcccgaaa taaaggtttc aatacaacct tgtttaccgc
    35821 aataacaagg gacttcctcg cgataacgca gctcgtcttc atccatccac ggtagcggat
    35881 tgtgtcccca ctcgcctgcc gtgccattgc cgccgatatg cgcccgccca tttaatgcca
    35941 cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac cgtctgcgct cccgctgccg
    36001 cgccatctac tgcttctgaa accgccagac agttggcatc atttgccagc cgcacttccc
    36061 gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag gttgaattgg
    36121 cattcttcac cacaccggtg tagggcgaaa tcgaacctgg aatgcccata cctaccgttc
    36181 cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt tcaatagtct
    36241 gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc cctgcatcgc
    36301 ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc actgtactct
    36361 ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca atgcaaggcc
    36421 gccgacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa attatcatgc
    36481 tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag
    36541 agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag gacatggcga
    36601 agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac gttgccaatg
    36661 gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac
    36721 aggcgctgga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa
    36781 ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gctgccgcgt gctttcagcc
    36841 gttttagcca gacaatgatg tggatcgaca cggttaacgg tttgattatg gtggactgcg
    36901 ccagtgccaa aaaagcggaa gatacgctgg cattactgcg taaaagcctg gggtcgttac
    36961 cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa tgggttcgct
    37021 ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc cgagctgaaa tcgttgctgg
    37081 aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag atcaccaatc
    37141 acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag cgcattcagt
    37201 ttgtgatgtg cgacgatggt tcgctcaagc gtcttaagtt ctgcgacgag ctgcgcgatc
    37261 aaaacgaaga tatcgaccgt gaagatttcg ccctgcgttt tgatgccgat ttcatcctga
    37321 tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt ggcgaagcac
    37381 aacgttaatt gctgattttc ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc
    37441 gtacgaagcc acaccaggca tataattatt cgctacggcg agcaataatt tttagcgcag
    37501 caatattatg cgttttacgc cgtaactgat ctaatcgcct catccctatt cgcctgtcag
    37561 cagcacgcag aatattcact aacccccatt tatcgctggt aaattgtttt gttaacagct
    37621 ccgcttttgg ggtatcaata tattcacgca ataaatcaga aattgaacaa atatccgtca
    37681 ggtaaggata ccagcaagtt tcaccgtgaa aattacgcac tccagcgtta cctgcaataa
    37741 agtctttcgg gtaatcccag atcacgtttt tatccaacgt gatccaataa cgcggtaaat
    37801 cggtacttcc accattttgg ctgcgcatga gataacgggt acagtgaatt tgcaggttga
    37861 tcgttggcgt cagtaagtca tagatctccc gctgtaactt gctccatgga cgttgtgtca
    37921 ttgtttttcc tcaagccggt gacgcgcttc actgcgaatt ttccgcgttg ccccggtttt
    37981 cactaaatca cgtaactgcg aaagatcggg ttgttcgaca aataagagta atgatagatc
    38041 ctcttttaac cacgccgttt tcacatcggc agccagctgt ggattattaa tagccagcga
    38101 ccgatcctga gactcagtca atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa
    38161 tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc attcatcatc
    38221 tgatttatct tttatcgtag ccagttccgc aaaaaatacc tggagatctt cactgatatt
    38281 tatatcaacc ttgatgaatt atgaatatat cggaaattga aaaaatcgca gcaaagga
//
LOCUS       DAZRVY010000027        38208 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZRVY010000027 DAZRVY010000000
VERSION     DAZRVY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.027"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            85..159
                     /locus_tag="ACHXOX_001424"
     tRNA            85..159
                     /locus_tag="ACHXOX_001424"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Gln,seq:ctg)
     gene            complement(313..1488)
                     /gene="ubiF"
                     /locus_tag="ACHXOX_001425"
     CDS             complement(313..1488)
                     /gene="ubiF"
                     /locus_tag="ACHXOX_001425"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR7004932.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1634..3058
                     /gene="miaB"
                     /locus_tag="ACHXOX_001426"
     CDS             1634..3058
                     /gene="miaB"
                     /locus_tag="ACHXOX_001426"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR7004933.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3211..4251
                     /locus_tag="ACHXOX_001427"
     CDS             3211..4251
                     /locus_tag="ACHXOX_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR7004934.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4248..4715
                     /gene="ybeY"
                     /locus_tag="ACHXOX_001428"
     CDS             4248..4715
                     /gene="ybeY"
                     /locus_tag="ACHXOX_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR7004935.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4805..5683
                     /gene="corC"
                     /locus_tag="ACHXOX_001429"
     CDS             4805..5683
                     /gene="corC"
                     /locus_tag="ACHXOX_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR7004936.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5708..7246
                     /gene="lnt"
                     /locus_tag="ACHXOX_001430"
     CDS             5708..7246
                     /gene="lnt"
                     /locus_tag="ACHXOX_001430"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR7004937.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7324..9201)
                     /locus_tag="ACHXOX_001431"
     CDS             complement(7324..9201)
                     /locus_tag="ACHXOX_001431"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR7004938.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9656..10564
                     /gene="gltI"
                     /locus_tag="ACHXOX_001432"
     CDS             9656..10564
                     /gene="gltI"
                     /locus_tag="ACHXOX_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR7004939.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10734..11474
                     /gene="gltJ"
                     /locus_tag="ACHXOX_001433"
     CDS             10734..11474
                     /gene="gltJ"
                     /locus_tag="ACHXOX_001433"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR7004940.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11474..12148
                     /gene="gltK"
                     /locus_tag="ACHXOX_001434"
     CDS             11474..12148
                     /gene="gltK"
                     /locus_tag="ACHXOX_001434"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR7004941.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12148..12873
                     /gene="gltL"
                     /locus_tag="ACHXOX_001435"
     CDS             12148..12873
                     /gene="gltL"
                     /locus_tag="ACHXOX_001435"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR7004942.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12991..13926
                     /gene="rihA"
                     /locus_tag="ACHXOX_001436"
     CDS             12991..13926
                     /gene="rihA"
                     /locus_tag="ACHXOX_001436"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR7004943.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            14018..15688
                     /gene="hscC"
                     /locus_tag="ACHXOX_001437"
     CDS             14018..15688
                     /gene="hscC"
                     /locus_tag="ACHXOX_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR7004944.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15748..17199)
                     /gene="djlC"
                     /locus_tag="ACHXOX_001438"
     CDS             complement(15748..17199)
                     /gene="djlC"
                     /locus_tag="ACHXOX_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR7004945.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17196..17903)
                     /locus_tag="ACHXOX_001439"
     CDS             complement(17196..17903)
                     /locus_tag="ACHXOX_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR7004946.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            18005..18559
                     /locus_tag="ACHXOX_001440"
     CDS             18005..18559
                     /locus_tag="ACHXOX_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7004947.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18569..19996)
                     /locus_tag="ACHXOX_001441"
     CDS             complement(18569..19996)
                     /locus_tag="ACHXOX_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR7004948.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19993..20700)
                     /locus_tag="ACHXOX_001442"
     CDS             complement(19993..20700)
                     /locus_tag="ACHXOX_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR7004949.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20864..21841
                     /gene="ybeQ"
                     /locus_tag="ACHXOX_001443"
     CDS             20864..21841
                     /gene="ybeQ"
                     /locus_tag="ACHXOX_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR7004950.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(21911..22393)
                     /locus_tag="ACHXOX_001444"
     CDS             complement(21911..22393)
                     /locus_tag="ACHXOX_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR7004951.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22628..25210
                     /gene="leuS"
                     /locus_tag="ACHXOX_001445"
     CDS             22628..25210
                     /gene="leuS"
                     /locus_tag="ACHXOX_001445"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR7004952.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25225..25806
                     /gene="lptE"
                     /locus_tag="ACHXOX_001446"
     CDS             25225..25806
                     /gene="lptE"
                     /locus_tag="ACHXOX_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR7004953.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25806..26837
                     /gene="holA"
                     /locus_tag="ACHXOX_001447"
     CDS             25806..26837
                     /gene="holA"
                     /locus_tag="ACHXOX_001447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR7004954.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26839..27480
                     /gene="nadD"
                     /locus_tag="ACHXOX_001448"
     CDS             26839..27480
                     /gene="nadD"
                     /locus_tag="ACHXOX_001448"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR7004955.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27504..28115
                     /locus_tag="ACHXOX_001449"
                     /pseudo
     CDS             27504..28115
                     /locus_tag="ACHXOX_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28375..28692
                     /gene="rsfS"
                     /locus_tag="ACHXOX_001450"
     CDS             28375..28692
                     /gene="rsfS"
                     /locus_tag="ACHXOX_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR7004956.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28696..29163
                     /gene="rlmH"
                     /locus_tag="ACHXOX_001451"
     CDS             28696..29163
                     /gene="rlmH"
                     /locus_tag="ACHXOX_001451"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR7004957.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29194..31095
                     /gene="mrdA"
                     /locus_tag="ACHXOX_001452"
     CDS             29194..31095
                     /gene="mrdA"
                     /locus_tag="ACHXOX_001452"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR7004958.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31098..32210
                     /gene="mrdB"
                     /locus_tag="ACHXOX_001453"
                     /gene_synonym="rodA"
     CDS             31098..32210
                     /gene="mrdB"
                     /locus_tag="ACHXOX_001453"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR7004959.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32221..33309
                     /gene="rlpA"
                     /locus_tag="ACHXOX_001454"
     CDS             32221..33309
                     /gene="rlpA"
                     /locus_tag="ACHXOX_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR7004960.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33448..34659
                     /gene="dacA"
                     /locus_tag="ACHXOX_001455"
     CDS             33448..34659
                     /gene="dacA"
                     /locus_tag="ACHXOX_001455"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR7004961.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34769..35032
                     /gene="ybeD"
                     /locus_tag="ACHXOX_001456"
     CDS             34769..35032
                     /gene="ybeD"
                     /locus_tag="ACHXOX_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR7004962.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35133..35774
                     /gene="lipB"
                     /locus_tag="ACHXOX_001457"
     CDS             35133..35774
                     /gene="lipB"
                     /locus_tag="ACHXOX_001457"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR7004963.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            36034..36987
                     /locus_tag="ACHXOX_001458"
     CDS             36034..36987
                     /locus_tag="ACHXOX_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR7004964.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37196..38161
                     /gene="lipA"
                     /locus_tag="ACHXOX_001459"
     CDS             37196..38161
                     /gene="lipA"
                     /locus_tag="ACHXOX_001459"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR7004965.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9575 a         9748 c         9589 g         9296 t
ORIGIN      
        1 atggggtcac aggttcgaat cccgtcgtag ccaccaaatt ctgaatgtgt cgaatacgtt
       61 cggcaaattc aaaaaccaat ttgttggggt atcgccaagc ggtaaggcac cggattctga
      121 ttccggcatt ccgaggttcg aatcctcgta ccccagccac attaaaaaag ctcgcttcgg
      181 cgagtttttt gcttttctgc gttcattcaa tgtcgaatgc gatgttgaca cgtcttatcc
      241 ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct acggttggca cgcatccggc
      301 aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc tgacgtttca acacgccagc
      361 acgctctgcc gccattaacc cgagattacg cacaaaacgc agcggcggca gattattact
      421 gaatccggca taaaacagat ccataccact ttgcataatg aagttatccg ccatgcgccg
      481 catctggtaa cgcttgagga caggataact ggcccacgct tcaccgtagc tgcgggcatt
      541 caccagaaca tcaatcaggg catcgacatc acgataacca agattcaccc cctgccccgc
      601 cagcggatgg atggtatgcg cggcatcgcc caccagcgca agccccggct gcacatactg
      661 caacgcatgt cggcgcgtca gcggaaacgc accagcggca agcggtgtaa cgtaacccag
      721 acgcgacggg aaatgcttcg cgatttccgc ctggaattgc gccatattca tattctgcaa
      781 ctggcgaata cgcgccggag agtcatacca caccagcgat gcccagttat caaacaacgg
      841 cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag gtgctgtcgc cgggatcgtt
      901 ctcacactgt acgctaatca acatgcacga ctgcgcatac tgccaggcat gaacgccaat
      961 tcccgccatc tgccgcacct gagaatttgc gccgtcggca ccaatcacca gtttcgcgcg
     1021 gatcgtttca ccgcccttca gttccagctc ctgaagatca ttatctcgat gtaatgcaat
     1081 cagcgagcct ggcacacgta acgttacttt cggatgcgct tccagcgcct gccacagcgc
     1141 ctgttgcagg acggtgtttt ccaccatata gccaagcaac ggtagcttaa gttcagcggc
     1201 gtcaaacacc acatgcgccg tttcccactc ccacgtttcc agtctgcggt aaggatggca
     1261 acgcatagcc tgtactgcat cccagacccc taacccttta agcaatgata ccgatgcggc
     1321 gctgatcgcc gagatccgca cgtccggttg gctatcagcg acaaacggtg caggttctgc
     1381 gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc cccagcgcca gcgcgccgcc
     1441 gaccattcct ccgccgacaa tggcaatttc cgttggttga tttgtcatgg tcaatcatcc
     1501 tgttagcaat atgctttaag tttacaggat ttttaccccc aagggctgat aacgctcata
     1561 ctggtcacaa cggcagcaaa gcattacact atacgccctg cattcctggc tactatttcg
     1621 caagagcaag tcgatgacca aaaaactcca tattaaaacc tggggctgtc agatgaacga
     1681 gtacgattca tcgaagatgg ccgatctgct ggatgccacc cacggctatc aactgaccga
     1741 cgtggcggaa gaagcggatg tgctgctgct gaacacctgc tcaatccgcg agaaggctca
     1801 ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta aaagagaaga atccagacct
     1861 gattatcggc gtcggtggct gcgtggcatc gcaagaaggc gagcacattc gccagcgcgc
     1921 ccactatgtc gatattattt ttgggccgca aacgctgcac cgtctgccgg agatgatcaa
     1981 ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc ttcccggaaa tcgagaagtt
     2041 tgaccgtcta ccggaaccgc gcgccgaagg gccgaccgcg tttgtctcca tcatggaagg
     2101 ctgcaataaa tattgcacct actgcgtggt gccttacacc cgtggtgaag aggtaagccg
     2161 tccgtctgac gatattctgt ttgagattgc ccagcttgcg gctcagggcg tgcgtgaagt
     2221 caacctgctc ggtcagaacg tgaacgcctg gcgtggtgaa aactacgacg gctccaccgg
     2281 atcgtttgcc gatctgctgc gtctggttgc tgcgatcgac gggatcgatc gtattcgctt
     2341 taccaccagc catccgatcg aattcaccga cgatatcatc gaagtgtatc gcgacacgcc
     2401 ggagctggtg agcttcctgc atctgccggt acaaagcggt tccgatcgca ttctgaacct
     2461 gatgggccgt actcacacgg cgctggagta caaagcgatc atccgtaaac tgcgtgcggc
     2521 gcgtccggat attcagatca gttctgactt cattgttggc ttccctggcg aaaccaccga
     2581 agacttcgag aaaacgatga agctgattgc cgacgtcaat ttcgacatga gctacagctt
     2641 tatcttctct gcacgtccgg gtacaccagc cgccgatatg gttgatgatg ttccggaaga
     2701 agagaagaag caacgtctgt atattctgca agaacgtatc aatcagcaag cgatggcatg
     2761 gagccgccgg atgctcggca ccacccagcg tattctggta gaagggactt cgcgtaagag
     2821 catcatggag ctttccgggc gtaccgaaaa taaccgcgtg gtcaacttcg agggcacacc
     2881 ggatatgatc ggtaaattcg tcgatgtaga aattaccgac gtctacccga actctctgcg
     2941 cggtaaagtg gtgcgtactg aagatgaaat gggtctgcgc gtggcagaaa caccggaatc
     3001 ggtgattgcc cgtacccgca aagaaaacga ccttggcgtg ggttattatc agccgtaatt
     3061 ctcaggcccg ccgttccggt gggccttgtt ttttctctct ttatccccat ctttatcgca
     3121 atgcttgcgc ctttttcctg tggcgtgaat aatttcctta taggccggtg aatgttcagc
     3181 ttccggcggc atacagataa gaggaacggt ttgaacatag acactcgcga aatcaccctg
     3241 gagccagcag acaacgcgcg tctgttgagc ctgtgcggcc cgtttgatga caacatcaag
     3301 cagctcgaac gccgtctcgg catcgagatc aatcgccgcg acaaccactt taaactgacc
     3361 ggccgtccga tttgcgtcac cgctgcggca gacattctgc gtagcctgta tgtcgataca
     3421 gccccgatgc gcggtcagat tcaggatatc gaaccggaac agatccacct tgcgattaaa
     3481 gaagcgcgag tactggagca aagtgcggag agcgtgccgg agtacggcaa agcggtcaat
     3541 atcaaaacca aacgcggcgt aattaagccg cgcacgccaa accaggcgca gtacatcgcc
     3601 aatattctcg accatgacat taccttcggc gttggcccgg cgggtacggg taaaacctac
     3661 ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg agattcgccg tattctgctg
     3721 actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc tgcctggcga tttaagccag
     3781 aaagtagacc cgtatctgcg cccactgtac gacgcgctgt ttgaaatgct gggctttgag
     3841 aaagttgaga aactgattga gcgcaacgtt attgaagtcg cgccgctggc ctatatgcgt
     3901 ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga gccagaacac caccatcgaa
     3961 cagatgaaga tgttcctgac ccgtatcggt tttaactcaa aagcggttat caccggcgac
     4021 gtcacacaga tcgacctgcc gcgtaatact aaatcaggct tacgtcacgc catcgaagtg
     4081 ctggccgatg tcgaagagat cagctttaac ttcttccaca gcgaagacgt ggttcgtcac
     4141 cctgtggtgg cgcgtatcgt taacgcctat gaagcctggg aagaagccga acaaaaacga
     4201 aaagcggcgc tggcggcaga acgcaagcgc gaagaacagg aacaaaaatg agtcaggtga
     4261 tcctcgattt acaactggca tgtgaagata attccgggtt accggaagag agccagtttc
     4321 aaacatggct gaatgcggtg atcccgcagt ttcaggaaga atcggaagtg actattcgcg
     4381 tggtcgatac cgccgaaagc cacagtctga atctgaccta tcgcggtaag gataagccga
     4441 ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat ggaaatgtcg ctactgggcg
     4501 atctggttat ctgccgtcag gtggttgaga aggaagctca ggagcaaggc aaaccactgg
     4561 aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca tttgttaggt tacgatcaca
     4621 tcgaagatga cgaagcagaa gaaatggaag ccctcgaaac agagattatg cttgctctgg
     4681 gctatgagga tccgtacatt gccgagaaag aataatttgc caggcatttg actggcagac
     4741 gtccccgccg ttggtgatta acgacggcgt tttgattaac taacatgaga acccatacga
     4801 cgccatgagc gacgacaatt cacacagtag tgacacgata agcaacaaga agggattttt
     4861 ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa aaccgtgacg aactgctggc
     4921 gctgatccgt gattccgggc agaacgacct tatcgacgaa gatacgcgcg atatgctcga
     4981 aggggtgatg gacatcgcag accaacgcgt tcgcgacatc atgatccccc gctcccagat
     5041 gattaccctg aaacgcaacc agacgctgga cgaatgcctt gatgtcatca tcgagtccgc
     5101 ccactcacgt ttcccggtga ttagcgaaga caaagatcac attgaaggga ttctgatggc
     5161 gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc ttcagcatgg acaaagtgtt
     5221 acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac cggatgctga aagagtttcg
     5281 ctctcagcgt taccacatgg cgatcgtcat tgacgaattc ggtggggttt ccggtctggt
     5341 gaccattgaa gacatcctgg aactgattgt tggtgagatt gaagacgagt atgacgaaga
     5401 agatgatatc gacttccgtc agctgagtcg tcatacctgg accgtgcgtg cactggcttc
     5461 cattgaagac ttcaacgaag cgttcggcac ccactttagc gatgaagagg ttgacactat
     5521 cggtggtctg gtgatgcagg catttgggca tcttccggcg cgtggcgaaa ctatcgacat
     5581 cgacggttac cagttcaaag tggcgatggc cgacagtcgg cgtattattc aggttcatgt
     5641 caaaatcccg gatgactcac cccagccgaa gctggatgaa taaaaccgaa actggataga
     5701 taactacatg gcttttgcct cattaattga acgccagcgc attcgcctgc tgctggcgtt
     5761 attattcggt gcctgcggaa cgctggcctt ctctccttac gacgtctggc ctgcggcgat
     5821 tatttcgttg atggggcttc aggcgttgac ctttaaccgc cgtccactcc agtctgccgc
     5881 tattggcttt tgctggggat ttggcctctt tggcagcggt attaactggg tctatgtcag
     5941 catcgcgacc tttggcggaa tgcctggccc ggttaacatc ttcctggtgg tgctgctggc
     6001 ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg ctgtcgcgtc tgtggccgaa
     6061 aaccacctgg ctgcgcgtgg cgattgccgc ccctgctctc tggcaagtga ccgagtttct
     6121 gcgcggttgg gtactgacag gcttcccgtg gttacagttc ggctatagcc agattgatgg
     6181 cccgttaaaa gggctggcac cgataatggg cgtggaagcc attaacttcc tgctgatgat
     6241 ggttagtggc ctgctggcac tggcgttggt caaacgcaac tggcgtccgc tggtggtggc
     6301 cgtcgtgctg tttgccctac ccttcccgct gcgttacatc caatggttta ccccgcaacc
     6361 ggagaaaacc attcaggttt cgatggttca gggcgatatt ccgcaatcac tgaaatggga
     6421 tggagatcag cttcttaata cgctgaagat ttactacaac gcaacggctc cgctgatggg
     6481 caaatcatcg ttgattatct ggccggagtc ggcgataacc gatctggaaa ttaatcagca
     6541 accgttcctc aaagcactgg acggtgagtt gcgcgataaa ggtagctcgc tggtaaccgg
     6601 gattgtcgac gcgcgtctca ataagcagaa ccgctacgat acctacaaca ccatcatcac
     6661 actgggtaaa ggtgcaccat acagctacga atcagccgat cgctataaca aaaaccatct
     6721 agtgccgttt ggcgagtttg tcccgctgga gtcgattctg cgtccgttag caccgttctt
     6781 tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc cagccgccgc tgtcggtaaa
     6841 tggtattgag cttactgcgg ctatttgcta cgagatcatt ctcggcgagc aagtgcgcga
     6901 taacttccgc ccggataccg actatctgct gactatctcc aacgatgcgt ggtttggtaa
     6961 gtctattggt ccatggcagc acttccagat ggcgcgaatg cgtgcgctgg agctggcgcg
     7021 cccactgttg cgcagcacca acaacggcat tacggcggtg attggcccgc agggggaggt
     7081 tcaggcgatg atcccgcagt tcacccgcga ggtgttgacc actaatgtga caccgaccac
     7141 cggactcaca ccgtatgcac gtaccggcaa ctggccactg tgggtgctga cggcgctgtt
     7201 tggttttgcc gctgtgttga tgagtctgcg agcacgtaaa cgttgatgtg cctgatgtat
     7261 cctgcatgag caaaacgtac tttctcattg agctttgggg catggttatc atgccccttt
     7321 ttcttacggt tcagggatac aagccacatt gccatattgg ggatgacgaa taattgcctg
     7381 cggatcaagc gagcgcgtcc tgtccatttg gatcaacccg ttcttcgcca tgacaaatat
     7441 ttttctacca tccgctgaaa aacgaaggtg attaacgtag ctggcaggat aaacgccaat
     7501 aatctgcccg gcattaatat tgagcaggat gacataacct tgcaggccat atgacaccaa
     7561 cgctaaatat tgaccgtccg ggctaatacc ctcaacggaa taaacgctcc tgctacacca
     7621 ttgcgtcata atgctggctt gttgatcccc tacgcttata cgtgaaatcc cctgactatc
     7681 ggcaaaaata acctcgccgt tttcaggtcg aaaacgcatc gccgatatcg ttccacgcgc
     7741 atcacctgcg ggaaagtctt ctttctgatg cgtgatggta ttgatccgca cgatgttacc
     7801 tctgcctgct cccggaacca gtaatgttct ttcatccggg gaaagtgcca taacccaggc
     7861 gcctggttta ccgctcgtcc ccgtcccgtc gtatttctcc agcgggatat ccgccacatg
     7921 ctgataagtc accagatcaa tcgctgacac actattactg gtgcgattca tcacccaggc
     7981 ttttgtgcca tcacgcgaga ggataaggct gtcaggatgt gcaccggtgg taatcgactg
     8041 aataatctcc cctgtcgcca cgttaatgac cgataacgca ttcgcttcaa atgaagtcgc
     8101 ataaatcaac ttttcatccg ggcttaatgc cagcgatctg acgccctgat ctttacagcc
     8161 accacaatgt ttgactgact tttctttcgc tatgggcgct gtaaaggtat gcaggatttt
     8221 cttttgtccg aggtcgaaaa caacaatgct gttcgtgttt tcaattgcgg catacaacat
     8281 attaccatcc cgactgatcg ccatatcatg gatatcggca atctcagcga gcaaatcacc
     8341 actggcctgt tcacgactta ccggagcgga taatgtttcc ctgagggcgt tgcgttcctc
     8401 cgctaactgc tgcttttttt gccgtcgctc ttgttcaatc tcctgtggat aaaacgcttc
     8461 acgcaggctt tgcctgacct gtaaaactga ctcatccatc tgctgctgcg caagccagat
     8521 agccccggtc agaagaagac tgcccgcaac aataatcccg ccttccgtaa cgagtcggtt
     8581 ttgcccagat aaacgcgcgc tcagccaacc caacgcgcca cccgcaatca ggccgccaat
     8641 gtggcaagcg ttatctattc ctgtttgccg ggcaccgtac aacagtgtca gcgccaccat
     8701 acccaacaga ggaaatactc gccgctggtt tttatggtat gtgcccgtac ctgaagcaaa
     8761 ctgtgttgct atcgctgcac cagctattcc cagaatcgcc ccggatgccc caacgctgat
     8821 gactatctgc tcactttcgc gtaacgcata acaggcgctg atgagcccac caaagacgcc
     8881 ggagaataac caggtaatca ggagtcgcca tttccccaga atctgttccg ccacgcctcc
     8941 cacgaccagg aatgcaaaca tattcatcag cagatgagaa acactactgt gcagaaagat
     9001 actgctcaat aagcgccagg gttcacccga caaagtaaga ggcgcaacgt ttgcgccgag
     9061 atggataagt gcggattgat cgacctcttc ataactcatc cattgtgcga gataacaaaa
     9121 tatcccaata cagagtgcaa aaaggataag ggttattgcg ggtaattgaa catttaacgg
     9181 cttaaccgaa gatgcagaca taaggtgttt cttccttgaa aagacaggta aaaagcattt
     9241 ttcccgtgtg cagttatcat cccggcaaac gtcccatgcc gaaaagataa ttctgtccga
     9301 aatatgactt tttcagccta aaattacaat cgtaattaat tcgcccctaa gtattttttg
     9361 gcatgtaagt tgctttattt actgtgtaag cgatgaaatc agccgcgacg caaccaggtt
     9421 gcaccaccac aaatcagcgg tagcaccaaa aaggtgcggt ttaagaattt tgcttcggaa
     9481 tggtgcggcg catctcgcaa aaacaaacaa taaaacagca atatctttac atttagccaa
     9541 actaaatgtt acctaacagg cacaacactg cacaataaag ttgcagacga taacaacaca
     9601 aacactcaca acgggtatcc atgcgttctt aacgcagaag ataaaggagt tggatatgca
     9661 attacgtaaa cctgccacag caatcctcgc cctggcgctt tccgcaggac tggcacaggc
     9721 agatgacgcc gccccggcag caggcagtac gctggacaaa atcgccaaaa acggtgtgat
     9781 tgtcgtcggt caccgtgaat cttcagtacc tttctcttat tacgacaatc agcaaaaagt
     9841 ggtgggttac tcgcaggatt actccaacgc cattgttgaa gcagtgaaaa agaaactcaa
     9901 caaaccggac ttgcaggtaa aactgattcc gattacctca caaaaccgta ttccactgct
     9961 gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc aacaacgtcg aacgccaaaa
    10021 acaggcggct ttctctgata ctattttcgt ggtcggtacg cgcctgctga ccaaaaaggg
    10081 cggcgatatc aaagattttg ccgacctgaa agacaaagcc gtagtcgtca cttccggcac
    10141 tacctctgaa gttttgctca acaaactgaa tgaagagcaa aaaatgaata tgcgcatcat
    10201 cagcgccaaa gatcacagtg actctttccg caccctggaa agcggtcgtg ccgttgcctt
    10261 tatgatggat gatgccctgc tggccggtga acgtgcgaaa gcgaagaaac cagacaactg
    10321 ggaaatcgtc ggcaagccgc agtctcagga agcctacggt tgcatgttgc gtaaagacga
    10381 tccgcagttc aaaaagctga tggatgacac caccgcgcag gtgcagacct ccggtgaagc
    10441 ggaaaaatgg tttgataaat ggttcaaaaa tcccattccg ccgaaaaacc tgaacatgaa
    10501 tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa ccgaatgaca aggcactgaa
    10561 ctaattacaa gaaccagggg cggaaattcc agccctctcg attgttacgt agcacggaca
    10621 gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa ccgcaatacg ctgggtaaca
    10681 atcttcgagg gtagcagtta acgctgctac cctctttttt ctggagtaga tttatgtcta
    10741 tagactggaa ctggggtatt tttttacaac aagccccgtt cggcaacacc acctatcttg
    10801 gttggatctg gagtggtttt caggtgacga tcgctttgtc gatctgcgcc tggattatcg
    10861 ctttcctcgt cggttcattt ttcggcattt tacgtaccgt tccaaaccgt tttctctccg
    10921 gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc gttgattgtg caattcttta
    10981 cctggtatct ggtgatcccg gagctgttgc cggagaaaat cggcatgtgg tttaaggccg
    11041 agctggatcc caatattcag tttttccttt catccatgct ctgcctgggg ctgtttaccg
    11101 ccgcccgtgt ttgcgaacag gtacgcgccg ccattcagtc gttgccgcgt gggcaaaaaa
    11161 atgccgcgct ggcgatgggc ctgacgctac cgcaggctta tcgatacgta ctgctgccta
    11221 atgcttatcg cgttatcgtc ccgccgatga cttccgagat gatgaacctg gtgaaaaact
    11281 ccgccatcgc ctcgactatt ggtctggtgg atatggcggc gcaggcgggt aaattgcttg
    11341 attactcagc ccacgcctgg gagtcgttta ccgctattac gctggcgtat gtgttgatta
    11401 acgcggttat catgctggta atgacgctgg ttgaacgtaa agttcgcctg cctggcaaca
    11461 tggggggcaa ataatgtacg agtttgactg gagttccatt gtcccttccc tgccatatct
    11521 gctcgacggg ctggtgatca cgctgaaaat caccgtcacg gcagtcgtga ttggtatttt
    11581 gtggggcacc atgctggcgg tgatgcgctt gtccagcttt gcgcccgtag cgtggtttgc
    11641 caaagcctac gttaacgtat tccgctcgat tcctttagtt atggttttgc tgtggtttta
    11701 cctgatcgtg ccaggttttc tgcaaaacgt gctgggatta tcaccaaaaa atgatatccg
    11761 tttaatttcg gcgatggtgg cgttttcgat gtttgaagcg gcctactatt cagagattat
    11821 ccgtgctggt attcaaagta tttctcgtgg tcagtcgagc gccgcgctgg cgttgggaat
    11881 gactcactgg cagtcgatga aactgattat tctgccgcag gcgttccgcg cgatggtgcc
    11941 gcttctgctc actcagggca tcgtactgtt ccaggatacc tcactggtgt atgtgttatc
    12001 tctggccgat ttcttccgta ccgcctcaac cattggtgag cgtgatggta cgcaggttga
    12061 gatgatcctg tttgccggat ttgtttattt cgttattagt cttagcgcgt cgttgttggt
    12121 cagctacttg aaaagaagga cagcataatg attaccctga aaaatgtttc aaaatggtat
    12181 ggtcactttc aggtgctgac cgactgctca accgaagtga aaaaaggcga agtggtggtg
    12241 gtttgcggcc cgtctggttc cggcaaatca acgctgatta aaaccgtcaa cggcctcgaa
    12301 ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg ttaatgacaa gaaaaccgat
    12361 ctggcaaagc tgcgttcccg cgtcgggatg gtattccagc atttcgagct gttccctcat
    12421 ctgtcgatta tcgaaaacct gaccctggcg caggtgaaag tgcttaaacg cgataaagcg
    12481 cccgctcgtg aaaaagcatt gaaattgctg gagcgcgtgg gactttccgc acatgccaat
    12541 aaatttccgg cgcagctttc cggcggtcag cagcagcgtg tggcaatcgc tcgcgcattg
    12601 tgtatggatc ctattgcgat gctgttcgac gaaccgacat cggcgctgga tccggagatg
    12661 atcaacgaag tgctggacgt gatggtggaa ctggcgaacg aaggaatgac catgatggtg
    12721 gtgacccacg aaatgggctt tgcccgcaaa gtggcgaatc gggtgatctt tatggacgag
    12781 ggtaaaattg tcgaagactc gccgaaagac gctttcttcg atgatccgaa atcggaccgc
    12841 gcgaaagact tcctcgcgaa aatcctgcat taatcactct ggcgcgcata cccaagtgcg
    12901 cgccatctca cgctttatcg tttcgcccct cgtatctgtt acatcctggc gttacccttg
    12961 tcgcaaagaa gcacacaaca aggagcaaca atggcactgc caattctgtt agattgcgac
    13021 ccaggtcatg acgacgctat cgcaatagtt ctcgccctcg cctcaccaga gcttgatgtc
    13081 aaagcaatta cgtcttccgc cggaaaccag acaccagaaa aaaccttacg caatgttctg
    13141 cgtatgctga ccttgcttaa tcgcaccgat attccggtag caggcggcgc ggtaaaaccg
    13201 ttaatgcgtg agttgattat cgcggacaat gtgcacggcg aaagcggtct ggatggcccg
    13261 gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg cggtagagct gatggcgaaa
    13321 acgctgcgcg aaagtgcgga acctgtcacg attgtgtcta ccggaccgca aactaacgtt
    13381 gccttgctgc tcaatagcca tccggaactg catagcaaaa ttgcccgtat cgtgattatg
    13441 ggcggcgcaa tggggcttgg taactggacg cctgcggctg aatttaatat ttacgttgac
    13501 ccggaagcgg cagaaattgt cttccagtca gggatcccag tggtgatggc cggtctggat
    13561 gttactcaca aagcacaaat ccacgttgaa gacaccgagc gtttccgcgc gattggcaac
    13621 cctgtttcaa ccattgttgc cgaactgctg gatttcttcc tcgaatatca taaagacgaa
    13681 aaatggggct ttgtcggtgc accgctgcat gacccatgca ccatcgcctg gctgttgaaa
    13741 cccgagttgt ttaccactgt tgagcgctgg gttggcgtgg aaacacaggg gaaatatagc
    13801 cagggtatga cggttgttga ttattattat ctgactggca ataaaccgaa tgccactgta
    13861 atggtcgatg ttgatcgtca gggctttgtt gatttactgg ctgatcgtct gaaattttac
    13921 gcttaacaca cgtcattgat taagatcacc acgcgttacc tgggtggtct taatctgttt
    13981 atctgtttat ctctttttct ttcaagatat aaatgcaatg gataatgcag aactcgccat
    14041 tggtatcgat ctcggcacta ccaatagttt aattgccgtc tggaaagacg gtgccgcgca
    14101 attaattcca aataagttcg gtgaatattt aacaccatcc ataattagca tggatgaaaa
    14161 taatcatatt ttagtcggaa aaccggctgt atcacggcgt acttcgcatc cggataaaac
    14221 ggcagcgtta tttaaacgtg caatgggcag taataccaac tggcggttag gcagcgacac
    14281 atttaacgcg ccagaactgt cctctttggt attacgctca ttaaaagaag atgccgaaga
    14341 atttctgcaa cgtccgatta aagatgtggt gatctccgtt ccggcttatt tcagcgatga
    14401 acaacgcaag catacccgtt tagcagcgga gttagccggg ttaaatgcgg tacgcttaat
    14461 taatgaaccc acagcagctg cgatggcata tggcctgcat acgcaacaaa atacccgctc
    14521 gctggttttt gatctcggtg gcggcacgtt tgacgtcacg gtgctcgaat acgccacgcc
    14581 ggtgattgaa gttcacgcct ccgctggcga taacttcctc ggtggcgaag attttaccca
    14641 tatgttggtc gatgacgttt taaagcgcgc ggatgtcgcc aggaccacgc ttaacgagag
    14701 tgaactggca gccttgtacg cctgtgtgga agcggcaaaa tgtagcaatc aatcgccatt
    14761 gcacattcgc tggcagtacc aggatgaaac gcgggagtgc gaattttacg agaacgaact
    14821 ggaagatttg tggttgccgc tgctcaatcg cttgcgagtg ccgattgaac aggcgttgcg
    14881 cgatgcacgt ctgaagccga gtcaaatcga cagtctggtg ctggttggcg gcgcgtcaca
    14941 aatgccgctg gtgcagcgaa tcgccgtgcg tctgtttggc aaattaccgt atcaaagtta
    15001 cgacccgagc accattgtcg cgctgggcgc agcaatccag gccgcctgcc gcttacgcag
    15061 tgaagatatt gaagaggtga tcctcactga tatttgccct tactcgttgg gcgttgaagt
    15121 taaccgccag ggcgtttccg gcattttctc gccaattatt gaacgaaaca ccacagtacc
    15181 cgtgtcgcgt gtagaaactt actcgaccat gcacccggaa caggattcta ttacggttaa
    15241 cgtctatcag ggagaaaacc acaaagttaa aaataacatt ctggttgaat ccttcgatgt
    15301 gccgttgaag aaaaccgggg cttatcagtc gattgatatt cgctttagtt atgatatcaa
    15361 cggcttgctt gaagttgacg tgcttctgga agatggcagc gttaagtcca gagtcattaa
    15421 tcatagcccg gtaacattga gcgcgcagca gattgaagag agtcggacgc ggttatccgc
    15481 attgaaaatt tatccgcgcg atatgctaat caaccgcacc tttaaggcca aactggaaga
    15541 gttgtgggcg cgggcgctgg gtgacgagcg agaagagatt ggccgggtga tcaccgattt
    15601 tgatgcggcg ttgcagtcaa acgatatggc ccgcgtcgat gaagttcgtc gacgggcgag
    15661 cgattatttg gctattgaga tcccttaaac acggctgccg gatgcggcat aaacgctgca
    15721 tccggcatcg gcgcaaaaaa cacatcctca gctaaacaac atcgcgattt ttccgagcgc
    15781 ataacctatc atcagcaccg ttaagctgat tttgataata ctggtaatgc tctgtggttt
    15841 ttcatcctca tcattttttt cttcatggtt ttcaggtgtt ggctcatcga catcttcatc
    15901 ctccggctcg agtttcactt tcgtcaacag catctgggtg ataatggcct ctaatggatg
    15961 agcatctcca ccttccagcc ttgtattaag acgggccagc atctcttgtt tcgggaacgc
    16021 ttccagcaca acctgttccg ctacccgata caaatccagt aacggtgaat cttgctctgt
    16081 ctcgctaccg actctcgatt tccaccacgc ccatgctttg cgcttgaaga ataacgactc
    16141 aataaacgat tgacctaacg gcgataattt tccgctatgt aaaatctccg cccataacag
    16201 ccgcgtccgg gcgctctcac caggaacata tttcaacggt ttaccttgcg catctaccat
    16261 gcttcgcgct tcgataacca tcactaataa cgcgaaataa tccgggcaat gacgcttgct
    16321 ccactgcaac aacaaattat cagcttgtgt agatgggtaa ctttcccagt acgcgcagag
    16381 atcggcaagc aggctttccc cctcgccgca gtacaaacgt tgtgcacact ggtaataccg
    16441 ggcatcttcg ctttctggtt cttccgcttg ccactgtttg gctacaggaa tgagttccgc
    16501 catacctgtt tgcaccgagc tgtaccagcg caacagtttg cgatgtaact ttgcatcatc
    16561 aggaatgaac cacggtcctt ccatcgccaa aaacgcagtg acatagtcag gatggtaacg
    16621 gcagcaacgt tccagcgcga agtaatattc aacagtctgg ttctgtaacg cgacgggata
    16681 ttccagaagg ctaaggaaat cgaaacaatc accagcctga atggcctcaa gaaaagcctc
    16741 cctttcttcc tcatccattc cttcgctgtc attaaacgaa tgccagttca aacgctctgc
    16801 caacaaagaa gcacaattta atgaaagata tcgcgcttct atagcgattg cacacagcgg
    16861 ccagcgtaat tgatcgacat cctccatgtt ccaggtattt aattgctgaa taaatttctg
    16921 ccaggcggag ggctgaaaac gatcactttc tgaatccagt aatgtcctga atgcacgtag
    16981 tatttcatgt tcagcggtgg catctttttc ttcatcatct gcttcctcga caggatttac
    17041 cgccagtcgc agcgcctctt cataggcctg gcgtaatgct ttaaacccct gcggatcggt
    17101 ttcgggatga cacaacggta agcgagcaag ataagcctgg cggataatgt ctatttgcgt
    17161 cgtgctttca atttcgagga tttgccaaca tgttttcata ggtcgctcac atccttaagc
    17221 gttaccgggc attcaggctg gacagttaat ggttgccagg gaagcgtggc atagaaacgg
    17281 tcgcttcccg ctgccagctc ttcgaacatc cgactcccgg cgctatcgta tttgtacagg
    17341 gttagcgcct cgcgtagcga agcgttgtct tcgcactgag aagattgcca gtagagacgc
    17401 cctagtgaat aggcagaaaa atagtgcatc cagctgtgat agtaatggtg agctcgtgca
    17461 tagacgcgcg attgcagcca taaactctct tcttccgtca gccagttgtt gagcacaccg
    17521 acccgtgaca gaaaccccat tcgaacataa tcccaggctt tgattccccc ttctccacag
    17581 cacatagcgg tttgggcaac gaattgggta taaatcaacc ctctctccga gccaccagca
    17641 attaatgctt tccactcttc aggggaagag cgttgccatg tcagatataa gccagctaaa
    17701 tcattggcat gaccatcatc agtcatctga taaatagtct gaattaaatc atcacgatta
    17761 tttattcccc aggatgatga gagatcgaat tcttttatcc cagtatacat ttttggcgag
    17821 tcatgcgcgg gtttgcaact ctgattaagt atttccatgg gcgcagaaag cgcaaaaaga
    17881 agatattgtt cttccttatt cattttttaa taatccctta agtgcattaa ctgactactt
    17941 ttttattatt gatgagtata aacttgacgc tttcaaaata aaaagaaaat cgaagcattc
    18001 acacatgaat aaaaaatcaa tgtatatatt cgcaattttt atagttgcag caattacctg
    18061 tattagccaa cccaagaaaa cgacgttgcg tgataaagcc atggtgaatt atgcctttga
    18121 ttatttaaga tcaccaggca gtctaccgtt aaccacggca gccacggagc tttccgcgat
    18181 tcatggtcac tcaacatcgc aatatcgcct tggcgaattt tatcttcatg gtagcgacgg
    18241 taaaccactg gattatacac aggcgagata ctggtatgag caatcagcag aacaggaaaa
    18301 tccgcgcgca caaagcaaac tggggtggat ctacctgaaa ggcctgggag tcaaacccga
    18361 tacccgtaaa gcaattctct ggtataagga agcagctgaa caagggtatg ctcatgctca
    18421 atatactttg ggtttgatct acagaaatgg cactggtatt aatgttaacc attatgaatc
    18481 tcaaaaatgg ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa gattacttgc
    18541 cggacttccc gcacattaac gtcgactgtc acccgaagag atggagtatt tttcctatca
    18601 aaccggcaaa gataaaaatg tagaaaataa ttttgatgac gcccagacag cccagcccgc
    18661 catcgtcaac gggcattggc tgcggttgcg gctccggctc gacggtttcc tgaccggtaa
    18721 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatctt
    18781 ctgcatcaag gcggctcaca atcggcatta atgattcttc tgtcggttgg ctggcctgca
    18841 ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg
    18901 agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagcgcag
    18961 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
    19021 ggacataaag tagctgttta ccctgatcgt taaccagttc acgagcttca atcatcatca
    19081 ccactaacgg gtaataatcg acccgatgct gacggcacca ttgcaatatt aaagcatccg
    19141 cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcaag ctttcgcctt
    19201 ctccacaata gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag
    19261 cttcccgcca cgaccgggcg taatccagaa gttcggggat atccaggcga cctgcgctat
    19321 accagcggag taaatttctg tgcagttttg catcatcggg gatgatcact gcgcgatgtt
    19381 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt
    19441 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa
    19501 aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
    19561 cgacgttttc ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
    19621 ataaatgggc agtgttcatg gcgattgtgc acagcgacca gcgtaattca tcaatctctt
    19681 ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
    19741 cgctatcaga ggcaagtaac gcgcgaaagg cgagcagaat ttcatgttct gctacctcat
    19801 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcctcctcat
    19861 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
    19921 gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt tcgaggatct
    19981 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
    20041 agccagtggt tgccaggaca aggcagggta atacgcatca tcactggcaa ataattgata
    20101 aaacatatca ttgccggaat cgtcgtattc tttacgagct aagaattcgc gtaataaagg
    20161 cagatgatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
    20221 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
    20281 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
    20341 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
    20401 ttgcgcataa gcctgacctt gctcatttag ttcagcaaga tagtcacgcc attcacatgg
    20461 cgaataacga aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat
    20521 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccatg aggaagaaag
    20581 gtcaaaaaag catgtaccta aatacatttt gggggatgaa tgcgaaggta aacagcattc
    20641 attgcgtatt tccatcggag tggaaagtgc aaacaatatt ttttgcgacc ccatgtccat
    20701 ataatttatc cttaaacatt catttaatga atgtaaacta catttcgaga ctcagttatt
    20761 tatacttttc tgacgcaaca taatacgcat aaaataaggc ttacgcaaaa atggaacaca
    20821 taaaaatata atatgtttta tctacaacca taaggaaatg ataatggttt ttacgtcaag
    20881 ttgctgcgat aatttatcaa tagatgagat tatcgaacgt gctgaaaaag gcgattgcga
    20941 ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgact ctccggacga
    21001 cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa ggtcattgtg aagcacagta
    21061 ttccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg agcaagccat
    21121 cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg
    21181 gacactggat cgtggagaag cccccaatta taaagaagcg gttgtctggt atcagatagc
    21241 cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa
    21301 cggagtcgca aaagactatg cgctggcatt tttctggtac aaacaagctg cattacaagg
    21361 ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca
    21421 aaacaagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcacgc
    21481 ccagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca
    21541 agcgatgtac tggtatctga aggctgccgc tcaggaaagc gtcggcgctt acgtcaacat
    21601 cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg
    21661 gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattatgta
    21721 tcactacgga gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt
    21781 tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt gagactgaag atttactgta
    21841 gagataaaaa caagggccgg ggaatttcat tcggcccata ttctctttat tgctgtgcga
    21901 aatcagacta ttacggctca aacgggcgtc tctggaactg gtcatgacca catttcgggc
    21961 atagcgtcag cacttccggt gtgtagatcg ggagatggaa gtgacatttc tcgcagacca
    22021 gatttcccag cccgaccact tctccgctgt gataaacccc atgatgattg aggtcctgga
    22081 aaacttcgcg ccattcaagc tgcgttttat cggtgatatc tgccagctcc tgccataagc
    22141 tttctttaat cacccgcata aagacgctgt cagattcttc tttcaggctc tcttcatagc
    22201 tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt cagctcatcg acctcggttc
    22261 gcgttaactc ccctgttttt attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat
    22321 cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac cagttcacgg taatattgag
    22381 caaccttgtt catcgtttca tctcccgggt cgttaactga ctataataat agacgttctt
    22441 tgttccttgc tttgctaata cgcccacaaa ctctgtaaat taatgtatga ataatccggg
    22501 catcatttca acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta
    22561 tgcggatctg aaaaaccacc tcaacgctac atttgtagcc gtattgaaaa caggaccact
    22621 ggctgccatg caagagcaat accgcccgga agagatagaa tccaaagtac agcttcattg
    22681 ggatgagaag cgcacatttg aagtaaccga agacgagagc aaagagaagt attactgcct
    22741 gtctatgctt ccctatcctt ctggtcgact acacatgggc cacgtacgta actacaccat
    22801 cggtgacgtg atcgcccgct accagcgtat gctgggcaaa aacgtcctgc agccgatcgg
    22861 ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg aaaaacaaca ccgctccggc
    22921 accgtggacg tacgacaaca tcgcgtatat gaaaaaccag ctcaaaatgc tgggctttgg
    22981 ttatgactgg agccgcgagc tggcaacctg tacgccggaa tactaccgtt gggaacagaa
    23041 attcttcacc gagctgtata aaaaaggcct ggtatataag aagacttctg cggtcaactg
    23101 gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt atcgacggct gctgctggcg
    23161 ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg tttatcaaaa tcactgctta
    23221 cgctgacgag ctgctcaacg atctggataa actggatcac tggccagaca ccgttaaaac
    23281 catgcagcgt aactggatcg gtcgttccga aggcgtggag atcaccttca acgttaacga
    23341 ctatgacaac atgctgaccg tttacactac ccgcccggac acctttatgg gttgtaccta
    23401 cctggcggta gctgcgggtc atccgctggc gcagaaagcg gcggaaaata atcctgaact
    23461 ggcggccttt attgacgaat gccgtaacac caaagttgcc gaagctgaaa tggcgacgat
    23521 ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac ccattaacgg gcgaagaaat
    23581 tcccgtttgg gcagcaaact tcgtattgat ggagtacggc acgggcgcag ttatggcggt
    23641 tccgggtcac gaccagcgcg actacgagtt tgcctctaaa tacggcctga acatcaaacc
    23701 agttatcctg gctgctgacg gctctgagcc agatctttct cagcaagccc tgactgaaaa
    23761 aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac catgaagcgg ccttcaacgc
    23821 catcgccgat aaactgactg cgatgggcgt tggcgagcgt aaagtgaact accgcctgcg
    23881 cgactggggt gtttctcgtc agcgttactg gggcgcgccg attccgatgg tgacgctgga
    23941 agacggtacc gtaatgccga ccccggacga ccagctgccg gtgatcctgc cggaagatgt
    24001 ggtgatggac ggcattacca gcccgattaa agcagatccg gagtgggcaa aaactaccgt
    24061 taacggtatg ccagcgctgc gtgaaaccga cactttcgac acctttatgg agtcctcctg
    24121 gtactatgcg cgctacactt gcccagagta caaagaaggt atgctggatt ccgaagcggc
    24181 taactactgg ctgccggtgg atatctacat tggtggtatc gaacacgcca ttatgcacct
    24241 gctctacttc cgcttcttcc acaaactgat gcgtgatgca ggcatggtga actctgacga
    24301 accagcgaaa cagttgctgt gtcagggtat ggtgctggca gatgccttct actatgttgg
    24361 cgaaaacggc gaacgtaact gggtttcccc ggttgatgct atcgttgaac gtgacgagaa
    24421 aggccgtatc gtgaaagcga aagatgcggc aggccatgaa ctggtttata ccggcatgag
    24481 caaaatgtcc aagtcgaaga acaacggtat cgacccgcag gtgatggttg aacgttacgg
    24541 cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg gctgatatga ctctcgaatg
    24601 gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa cgtgtctgga aactggttta
    24661 cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt gatgcgctga ctgaagatca
    24721 gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa gtgaccgatg atatcggccg
    24781 tcgtcagacc ttcaacaccg caattgcggc gattatggag ctgatgaaca aactggcgaa
    24841 agcaccaacc gatggcgagc aggatcgcgc tctgatgcag gaagcgctgc tggccgttgt
    24901 ccgtatgctt aacccgttca ccccgcacat ctgcttcacg ctgtggcagg aactgaaagg
    24961 cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac gaaaaagcga tggtggaaga
    25021 ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt gccaaaatca ccgttctggt
    25081 ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag gaacatctgg tagcaaaata
    25141 tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca ggtaaactcc tcaatctggt
    25201 cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa cattgttgtt atctctggcg
    25261 gtgttaatca ccgccgggtg tggctggcat ctgcgtgata ccacgcaggt tccttccact
    25321 atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc cattaagccg tgcagtgcgt
    25381 aaccagttac gtctgaatgg tgtcgagttg cttgataaag aaaccacgcg taaggacgtt
    25441 ccatccctgc gtttgggtaa agtgagcatc gcgaaagata ccgcatcggt attccgtaac
    25501 ggtcaaacag cagagtatca gatgatcatg acggtgaatg cgaccgtgtt gatccccggc
    25561 cgtgatatct acccgattag cgccaaagtc ttccgttcgt tctttgataa cccgcaaatg
    25621 gcgttagcga aagataacga acaagacatg atcgtaaaag agatgtacga ccgtgctgcc
    25681 gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg atattcgttc cgacgaagaa
    25741 cagacgtcga ccacaacgga tactccggca acgcctgcac gcgtctccac cacgctgggt
    25801 aactgatgat tcggttgtac ccggaacaac tccgcgcgca gctcaatgaa gggctgcgcg
    25861 cggcgtatct tttacttggt aacgatcctc tgttattgca ggaaagccag gacgctgttc
    25921 gtcaggtagc tgcggcacaa ggattcgaag aacaccacac tttttccatt gatcccaaca
    25981 ctgactggaa tgcgatcttt tcgttatgcc aggctatgag tctgtttgcc agtcgacaaa
    26041 cgctattgct gttgttacca gaaaacggac cgaatgcggc gatcaatgaa caacttctca
    26101 cactcaccgg acttctgcat gacgacctgc tgttgatcgt ccgcggtaat aaattaagca
    26161 aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa tcgcagcgtg caggtgacct
    26221 gtcagacacc ggagcaggct cagcttcccc gctgggttgc tgcgcgcgca aaacagctca
    26281 acttagaact ggatgacgcg gcaaatcagg tgctctgcta ctgttatgaa ggtaacctgc
    26341 tggcgctggc tcaggcactg gagcgtttat cgctgctctg gccagacggc aaattgacat
    26401 taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt cacccctttt cattgggttg
    26461 atgctttgtt gatgggaaaa agtaagcgcg cattgcatat tcttcagcaa ctgcgtctgg
    26521 aaggcagcga accggttatt ttgttgcgca cattacaacg tgaactgttg ttactggtta
    26581 acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt gtttgataag catcgggtat
    26641 ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg cttaagtcag acgcagttac
    26701 gtcaggccgt gcaactcctg acacgaacgg aactcaccct caaacaagat tacggtcagt
    26761 cagtgtgggc agagctgaaa gggttatctc ttctgttgtg ccataaaccc ctggcggacg
    26821 tatttatcga cggttgatat gaaatcttta caggctctgt ttggcggcac ctttgatccg
    26881 gtgcactatg gtcatctaaa acccgtggaa acgctggcga atttgattgg tctgacgcgg
    26941 gtcacaatca tccctaataa tgttcctccg catcgtcccc agccggaagc gaacagcgtg
    27001 cagcgtaaac acatgcttga actggcgatt gccgacaagc cattatttac tcttgatgaa
    27061 cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa cactgaaaga gtggcggcag
    27121 gaacaaggac cggacgtgcc gctggcgttt attattggtc aggattcact gctgaccttt
    27181 ccgacctggt acgaatacga aacgatactc gacaatgcac atttgatcgt ctgtcggcgt
    27241 ccaggttacc cacttgaaat ggcgcaaccg caataccagc aatggctgga agatcatttg
    27301 acacataacc cggaagatct tcaccttcag cctgccggta aaatttatct ggctgaaacg
    27361 ccgtggttta acatctcggc gaccatcatc cgcgaacgtt tgcaaaacgg tgaatcgtgt
    27421 gaggatttat tgccggaacc ggtactgact tacattaacc aacaaggctt gtatcgctga
    27481 tacctgtcgt tacggagggc atgatgcgac tgtggttaat tcgtcatggt gaaacgcaag
    27541 cgaatatcga tggtctttac agcggtcatg cgcccacccc cctgaccgcg cgcggtattg
    27601 agcaagcgca aaatctgcat acgctgctac atggtgtttc ctttgatctg gttttatgca
    27661 gtgaactgga acgggcacag cataccgcac gactggttct cagtgaccgc cagctccccg
    27721 tgcaaatcat ccctgaactc aacgaaatgt tttttggcga ctgggagatg cgacatcatc
    27781 gcgacctcat gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg
    27841 caatccccac gaacggtgaa ggatttcagg cattttcgca acgtgtggaa cgctttatcg
    27901 caaggcttag tgaatttcag cactatcaga atattttagt cgtcagccat cagggtgtac
    27961 tgagtctgtt aatcgcccgt ttaattggca tgcctgccga ggctatgtgg cattttcgcg
    28021 ttgactaggg gtgttggagc gccattgata tcaaccaaaa attcgcgacg ctacgcgtcc
    28081 tcaatagccg tgccatcggg gtcgagaatg catgactttt ctgttttttt acgggtaagc
    28141 cgcaacgacc attgacagac ccgggcaggc tgatattctc cgcagccaga cttttttcgc
    28201 cagacacgac tttgtagaaa ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt
    28261 gggatgatag cccactttcg aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg
    28321 ttcaggtata ctgacagacc atttttatct atttgattca cccaggggga aaacttgcag
    28381 ggtaaagcac tccaggattt tgttatcgac aaaattgatg acctcaaagg tcaggacatc
    28441 atcgccttag acgttcaagg caaatccagc atcaccgact gcatgatcat ctgtacgggt
    28501 acgtccagcc gtcatgttat gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg
    28561 ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg actggattgt cgtggatttg
    28621 ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc gcctgtatga actggaaaaa
    28681 ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg
    28741 gtacaaaccg gttttaccga gtacctgcgt cgttttccga aagatatgcc cttcgagctg
    28801 attgaaattc cggccggaaa acgcggcaag aatgcggaca tcaagcgcat actcgacaaa
    28861 gagggtgagc agatgttggc ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca
    28921 ggcaagccct gggatacgcc gcagttagcc gctgagctgg aacgctggaa gctggatggt
    28981 cgcgacgtca gtctactgat tggcgggcct gaagggttgt cgcctgcctg taaagcggcg
    29041 gcggagcaga gctggtcgct gtcggcgctt accctccccc atccgctggt tcgcgtgctg
    29101 gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca accatcctta tcaccgtgag
    29161 tgataaggga gctttgagta gaaaacgcag cggatgaaac tacagaactc ttttcgcgac
    29221 tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg
    29281 ctgctgaccg gcgtgcttat cgccaacctg tataatctgc aaattgttcg ctttaccgac
    29341 taccagactc gctctaatga aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc
    29401 attatctacg accgtaatgg tatccctctg gccctcaacc gcactatcta ccagatagaa
    29461 atgatgccag agaaagtcga taacgtgcag caaacgctgg acgctttgcg cagcgtggtt
    29521 gatctgaccg atgacgatat tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc
    29581 acctctattc cggtgaaaac taacctgacc gaagtacaag tagctcgctt tgccgtcaat
    29641 cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac gtcgttacta tccttacggt
    29701 tcggcgttga cccacgtcat cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc
    29761 ctgaataatg acggcaaact ggccaactat gcggcaacgc atgatatcgg taagctgggc
    29821 attgagcgtt actatgaaga cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt
    29881 aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac caccgcaagc cggacacgat
    29941 atttacctga cgctggatct caaactccag caatatattg aaacgctgct ggcgggtagc
    30001 cgcgcagctg tggttgtcac cgatccgcgt acaggtgggg tgctggcgct ggtttccacg
    30061 cctagttatg acccaaacct gtttgttgac ggtatctcca gcaaagatta ttccgccttg
    30121 ttgaacgccc cgaatacacc gctggtgaac cgcgccacac agggggttta tcctcccgcg
    30181 tctacagtta aaccctatgt ggcggtttcg gcattgagcg ccggggtgat cacgcgcaat
    30241 acgacgctgt ttgacccagg ctggtggcaa ctgccaggtt cggaaaaacg ttatcgtgac
    30301 tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat cgctggaaga atctgcggat
    30361 accttcttct atcaggtggc ctacgatatg gggatcgatc gcctctccga atggatgggt
    30421 aaattcggtt atggtcatta caccggtatc gacctggcgg aagaacgttc cggcaacatg
    30481 cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt
    30541 ccggttggta tcggtcaggg ttactggaca gcgaccccaa tccagatgag taaggcactg
    30601 atgatcctga ttaatgacgg tatcgtgaag gttcctcatt tgctgatgag caccgccgaa
    30661 gacggcaaac aggtgccatg ggtacagccg catgaaccgc ccgtcggcga tattcattcc
    30721 ggttactggg agctggcgaa agacggtatg tacggtgttg ctaaccgccc taacggtacg
    30781 gcgcataaat actttgccag cgcaccgtac aaaattgcgg cgaaatccgg taccgctcag
    30841 gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca aaattgccga gcgtttacgt
    30901 gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc cgcaagtggc tgtcgccatg
    30961 attctggaga acggtggtgc gggtccggcg gttggtacac tgatgcgcca gatcctcgac
    31021 cacattatgc tgggtgataa caacaccgat ctgcctgcgg aaaatccagc ggttgccgca
    31081 gcggaggacc attaatcatg acggataatc cgaataaaaa aacattctgg gataaagtcc
    31141 atctcgatcc cacaatgctg ctgatcttac tggcattgct ggtttacagc gccctggtta
    31201 tctggagcgc cagcggtcag gatattggca tgatggagcg taaaatcggc caaatcgcga
    31261 tgggtctagt catcatggtg gtgatggcgc aaattcctcc acgcgtttat gaaggctggg
    31321 ccccctatct ctatatcatc tgtattattt tgctggtggc ggtagatgct ttcggtgcca
    31381 tctctaaagg tgctcaacgc tggctggacc tcggtattgt tcgttttcag ccgtcggaaa
    31441 ttgccaaaat agccgtacca ctgatggttg cgcgctttat caaccgcgac gtttgcccgc
    31501 catcgttgaa gaacactggc atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg
    31561 ctgcacagcc tgacctggga acatcaatcc tcgttgcgct ttccggtctg tttgtactgt
    31621 tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt agtgctggtg gcggcgttca
    31681 ttccgattct gtggttcttc ctgatgcatg attaccagcg ccagcgcgta atgatgctcc
    31741 tagacccgga atcagaccca ctcggcgcgg gctatcacat tattcagtct aaaattgcta
    31801 ttggctccgg cggattacgc ggcaaaggct ggctgcacgg cactcagtca cagcttgaat
    31861 ttctccccga acgccatacc gactttatct tcgcggtact ggcggaagag ctgggattag
    31921 tgggcattct gattctgctc gctctctaca ttctgctgat catgcgcggg ctgtggatag
    31981 ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg tggcttaatg ctgatattat
    32041 tcgtttatgt cttcgtaaat attggtatgg taagcggtat tctgccggtt gtaggggttc
    32101 cgctcccact ggtcagttat ggaggatcgg cgctaattgt gctgatggct gggttcggga
    32161 ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca
    32221 atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc
    32281 gatgatggtc agcaacagac ggtaagtgta ccgcagcctg cggtatgtaa cggccccata
    32341 gttgaaatta gcggggcgga cccgcgtttc gaaccactga acgcgacggc aaatcaggat
    32401 taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg
    32461 ggactggcgg caatctatga tgccgaaccc ggcagtaacc tgacggcctc tggcgaagct
    32521 ttcgatccga cacagctgac ggcggcccat ccaacgcttc cgatccccag ctacgccaga
    32581 atcactaacc tggctaacgg gcgaatgatc gtggtgcgca ttaatgatcg cggcccttac
    32641 ggcaacgacc gcgttatttc actttctcgc gcggcagctg accgtcttaa cacgtcaaac
    32701 aacaccaaag ttcgtatcga tccgattatt gtcgcccagg atggttcgct ttctggtcct
    32761 ggtatggctt gtaccacagt cgccaaacag acttacgccc tgccagcacc tcccgattta
    32821 agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg gtgacattct tccggtcagt
    32881 aattcgacgc taaaaagcga agatccgacc ggcgcgccgg taaccagtag cggtttcctc
    32941 ggcgctccaa cgaccttagc gcctggtgta ctggaaggca gcgaaccgac gcctgcgcca
    33001 cagcccgttg ttacagctcc gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg
    33061 caagccgcct cgcaaagcgc cagcggcaac tttatggtgc aagtcggggc cgtaagcgat
    33121 caggctcgtg cgcaacagta ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc
    33181 gtaactcaaa atggcgcggt ctggcggatc cagcttggcc cattcgccag caaagccgaa
    33241 gccagtacct tgcagcaacg tttgcaaacc gaagcccaat tacagtcatt tattactacc
    33301 gcgcagtagc gtaaagcagg catctgatgt gtcaatttct gtaaatgacg ttaacaaagt
    33361 cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta tagtagggca cttttttaat
    33421 tccatcacgg atgtcgtagt tcagaccatg aataccattt tttccgctcg tatcatgaag
    33481 cgcctggcgc tcaccacggc tctttgcaca gcctttatct ctgctgcaca tgccgatgac
    33541 ctgaatatca aaactatgat cccgggtgta ccgcagatcg atgcggagtc ctacatcctg
    33601 attgactata actccggcaa agtgctcgcc gaacagaacg cagatgtccg ccgcgatcct
    33661 gccagcctga ccaaaatgat gaccagttac gttatcggcc aggcaatgaa agccggtaaa
    33721 tttaaagaaa ctgatttagt cactatcggc aacgacgcat gggccaccgg taacccggtg
    33781 tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc aggttccggt ttctcagctg
    33841 atccgcggta ttaacctgca atcgggtaac gatgcttgtg tcgccatggc cgattttgcc
    33901 gctggtagcc aggacgcttt tgttggcttg atgaacagct acgttaacgc actgggcctg
    33961 aaaaataccc acttccagac ggtacatggt ctggatgctg atggtcagta cagctccgcg
    34021 cgagatatgg cgctgatcgg ccaggcattg atccgtgacg taccgaatga atactcgatc
    34081 tataaagaaa aagaatttac gtttaacggt attcgccagc tgaaccgtaa cggcctgtta
    34141 tgggataaca gcctgaatgt cgacggcatc aaaaccggac acactgacaa agcaggttac
    34201 aaccttgttg cttctgcgac tgaaggccag atgcgcttga tttctgcggt gatgggcgga
    34261 cgtactttta aaggccgtga agccgaaagt aaaaaactgc taacctgggg cttccgtttc
    34321 ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg cctctgaacc ggtttggttt
    34381 ggtgattctg atcgcgcttc gttaggggtt gataaagacg tgtacctgac cattccacgt
    34441 ggtcgcatga aagatctgaa agccagctat gtgctgaaca gcagtgaatt gcatgcgccg
    34501 ctgcaaaaga atcaggtcgt cggaactatc aacttccagc ttgatggcaa aacgatcgag
    34561 caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta acttcttcgg caaaatcatt
    34621 gattacatta aattaatgtt ccatcactgg tttggttaaa aattgaacac ttgaaagtgt
    34681 aatttccgtc cccatatact aagcatcagt aaaaaactcc cgccttctgg cgggagttgc
    34741 tatttaatta cgttacgccg gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt
    34801 ccctactcct tttacttaca aagttatggg gcaggcgtta cctgagctgg ttgatcaggt
    34861 ggttgaagtg gtacagcgcc atgcgccagg tgactacacc ccaacggtaa aaccaagcag
    34921 caaaggcaac taccactcgg tatctatcac tatcaacgcc actcatatcg agcaggttga
    34981 aacactgtat gaagaactag gcaaaatcga tattgtccgc atggttctgt aactcgcttc
    35041 tccgttaccc ggcccctggt cgggtaactc ccctctcctc gctgtgatat acttttcccc
    35101 cacttttact cattctccac ggagatgcag ttttgtatca ggataaaatt cttgtccgcc
    35161 agctcggtct tcagccttac gagccaatct cccaggctat gcatgaattc accgataccc
    35221 gcgatgatag tacccttgat gaaatctggc tggtcgagca ctatccggta ttcacccaag
    35281 gtcaggcagg aaaagcggag cacattttaa tgccgggtga tattccggtg atccagagcg
    35341 atcgcggtgg gcaggtgact tatcacgggc cggggcaaca ggtgatgtat gtgttgctta
    35401 acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg
    35461 tgaataccct ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct
    35521 atgtcgggga aaagaaaatt tgctcactgg gtttacgtat tcgacgcggt tgttcattcc
    35581 acggtctggc attaaacgtc aatatggatc tttcaccatt tttacgtatt aatccttgtg
    35641 ggtatgccgg aatggaaatg gctaaaatat cacaatggaa acccgaagcg acgactaata
    35701 atattgcacc acgtttactg gaaaatattt tagcgctact aaacaatcct gacttcgaat
    35761 atattaccgc ttaattgcat acctcaatgg cccaatttac actgggtcat tactcataat
    35821 acctgctaat gctttacatc tccttcgcgt ttgtatactt tctttatcac ttgcagaaat
    35881 gcggtgctac gccttgctcc gtaactcacc ccattgatta acctattgaa atagttgatt
    35941 atggtgagca aaagcgttcc gttccaatta gacataagat tattgtaatt tttttaaata
    36001 ttatttttcc atgaataaaa ttggagtaag tgcgtggata gtaataatca aattgaaccc
    36061 tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat ttacaacctt acgaaatatt
    36121 gatcttaacc ttctgactat ttttgaagct gtatatgtac ataaagggat cgttaatgca
    36181 gcgaaagtgc ttaatctgac cccctcggca atcagtcagt ctattcagaa actgcgcgtt
    36241 atattccctg acccattgtt tattcgcaaa ggccagggtg tcactcctac cgcatttgcg
    36301 atgcatctac atgagtatat cagtcagggc cttgagtcca ttcttggcgc gctggatatc
    36361 gaaggaagct atgataagca acgaacgata actattgcga ccactccctc ggtcggagcc
    36421 ctggtccttc ctgtcatcta tcgggcgatt aaaactcact atccgcagct tttactgcgc
    36481 aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt ttcaaaccga tctcatcatc
    36541 gataacatgt tttgcaccaa tcgtacggtg caacatcatg ttctgttcac cgacaatatg
    36601 gtgttaattt gccgcgaggg aaatccacta ctctctttag aagatgacag agagactatc
    36661 gacaacgctg cgcatgtact cctgttaccg gaagggcaaa atttcagcgg tctgcggcag
    36721 agagttcaag agatgtttcc ggaccggcaa atcaatttca ccagctacaa cattttgaca
    36781 atcgctgcac tggttgccaa cagtgacatg ttagcgatta ttcccagccg tttttataac
    36841 ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc cgtccttaaa tgaggagcaa
    36901 atagatttct ctatccacta caacaaattc agcctgcgtg atcccatcct gcacggggta
    36961 atagatgtta tccgtaatgc attttgaaaa agtattgtta cagccagatc acagcgcaga
    37021 acgataaaat ggcacaatcc aacaacaatt ttacattttc gcgaccgctt tggctgcttt
    37081 caggtccgtt tcaatgatat actgccagtc gttaattcaa aaatagttga taattacaac
    37141 aatctattga attgaaacgc tttccttcgt aattcgcaac tggaacacgc acgctatgag
    37201 taaacccatt gtgatggaac gcggtgttaa ataccgcgat gccgataaga tggcccttat
    37261 cccggttaaa aacgtggcaa cagagcgcga agccctgctg cgcaagccgg aatggatgaa
    37321 aatcaagctt ccagcggact ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa
    37381 tggcctgcat tctgtctgcg aggaagcctc ctgccctaac ctggcggaat gcttcaacca
    37441 cggcacagca acgtttatga tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga
    37501 tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa ccagtgaaac tggcgcagac
    37561 cattgccgat atggcgctgc gttatgtggt tatcacctcc gttgaccgtg atgacctgcg
    37621 cgatggcggt gcccagcact ttgcggattg cattactgcc attcgggaaa aaagcccgca
    37681 aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt atggatcgtg ctctggatat
    37741 tctgactgca acgccaccag atgtgttcaa ccataacctg gaaaacgtac cgcgtattta
    37801 ccgtcaggta cggcctggtg cagattacaa ctggtcgctg aagctgctgg aacgctttaa
    37861 agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg gtgggactgg gtgaaaccaa
    37921 tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat ggtgtgacga tgttaacgct
    37981 ggggcaatat ttgcagccaa gccgccatca cctgccggtt caacgttacg ttagcccgga
    38041 tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg
    38101 tccgtttgtc cgctcttctt accacgccga tttgcaggcg aaagggatgg aagttaagta
    38161 atcctgtaat aattacttac atctgtctga taaaaaaccc gctttttg
//
LOCUS       DAZRVY010000028        36784 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZRVY010000028 DAZRVY010000000
VERSION     DAZRVY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.028"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHXOX_001460"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHXOX_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR7004966.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHXOX_001461"
     CDS             complement(280..873)
                     /locus_tag="ACHXOX_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR7004967.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHXOX_001462"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHXOX_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7004968.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHXOX_001463"
     CDS             1788..2183
                     /locus_tag="ACHXOX_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR7004969.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHXOX_001464"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHXOX_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR7004970.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHXOX_001465"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHXOX_001465"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR7004971.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHXOX_001466"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHXOX_001466"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR7004972.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHXOX_001467"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHXOX_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR7004973.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHXOX_001468"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHXOX_001468"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR7004974.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHXOX_001469"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHXOX_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR7004975.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHXOX_001470"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHXOX_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR7004976.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHXOX_001471"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHXOX_001471"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR7004977.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHXOX_001472"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHXOX_001472"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR7004978.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHXOX_001473"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHXOX_001473"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR7004979.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHXOX_001474"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHXOX_001474"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR7004980.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHXOX_001475"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHXOX_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR7004981.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHXOX_001476"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHXOX_001476"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR7004982.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHXOX_001477"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHXOX_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR7004983.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHXOX_001478"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHXOX_001478"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR7004984.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHXOX_001479"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHXOX_001479"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR7004985.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHXOX_001480"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHXOX_001480"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR7004986.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHXOX_001481"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHXOX_001481"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR7004987.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHXOX_001482"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHXOX_001482"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR7004988.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHXOX_001483"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHXOX_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR7004989.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHXOX_001484"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHXOX_001484"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR7004990.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHXOX_001485"
     CDS             complement(26207..26548)
                     /locus_tag="ACHXOX_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR7004991.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHXOX_001486"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHXOX_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR7004992.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHXOX_001487"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHXOX_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR7004993.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHXOX_001488"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHXOX_001488"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR7004994.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHXOX_001489"
     CDS             33227..34570
                     /locus_tag="ACHXOX_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR7004995.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHXOX_001490"
     CDS             complement(34667..34873)
                     /locus_tag="ACHXOX_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR7004996.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHXOX_001491"
     CDS             35198..35752
                     /locus_tag="ACHXOX_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR7004997.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHXOX_001492"
     CDS             complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHXOX_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR7004998.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8908 a         9852 c         9141 g         8883 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaagcg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    36781 ttat
//
LOCUS       DAZRVY010000029        36066 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZRVY010000029 DAZRVY010000000
VERSION     DAZRVY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.029"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHXOX_001493"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHXOX_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR7004999.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHXOX_001494"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHXOX_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHXOX_001495"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHXOX_001495"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR7005000.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHXOX_001496"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHXOX_001496"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR7005001.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHXOX_001497"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHXOX_001497"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR7005002.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHXOX_001498"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHXOX_001498"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR7005003.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHXOX_001499"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHXOX_001499"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR7005004.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHXOX_001500"
     CDS             8330..10048
                     /locus_tag="ACHXOX_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR7005005.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHXOX_001501"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHXOX_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR7005006.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHXOX_001502"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHXOX_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR7005007.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHXOX_001503"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHXOX_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR7005008.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHXOX_001504"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHXOX_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR7005009.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHXOX_001505"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHXOX_001505"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR7005010.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHXOX_001506"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHXOX_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHXOX_001507"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHXOX_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR7005011.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHXOX_001508"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHXOX_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR7005012.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHXOX_001509"
     CDS             complement(20443..20718)
                     /locus_tag="ACHXOX_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR7005013.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="ACHXOX_001510"
     CDS             complement(20927..22912)
                     /gene="yjcS"
                     /locus_tag="ACHXOX_001510"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR7005014.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23165..23482
                     /locus_tag="ACHXOX_001511"
     CDS             23165..23482
                     /locus_tag="ACHXOX_001511"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR7005015.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23551..23880
                     /gene="yjdP"
                     /locus_tag="ACHXOX_001512"
     CDS             23551..23880
                     /gene="yjdP"
                     /locus_tag="ACHXOX_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR7005016.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(24027..24785)
                     /gene="phnP"
                     /locus_tag="ACHXOX_001513"
     CDS             complement(24027..24785)
                     /gene="phnP"
                     /locus_tag="ACHXOX_001513"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR7005017.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(24787..25221)
                     /gene="phnO"
                     /locus_tag="ACHXOX_001514"
     CDS             complement(24787..25221)
                     /gene="phnO"
                     /locus_tag="ACHXOX_001514"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR7005018.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25208..25765)
                     /gene="phnN"
                     /locus_tag="ACHXOX_001515"
     CDS             complement(25208..25765)
                     /gene="phnN"
                     /locus_tag="ACHXOX_001515"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR7005019.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25765..26901)
                     /gene="phnM"
                     /locus_tag="ACHXOX_001516"
     CDS             complement(25765..26901)
                     /gene="phnM"
                     /locus_tag="ACHXOX_001516"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR7005020.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(26898..27578)
                     /gene="phnL"
                     /locus_tag="ACHXOX_001517"
     CDS             complement(26898..27578)
                     /gene="phnL"
                     /locus_tag="ACHXOX_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR7005021.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(27689..28447)
                     /gene="phnK"
                     /locus_tag="ACHXOX_001518"
     CDS             complement(27689..28447)
                     /gene="phnK"
                     /locus_tag="ACHXOX_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR7005022.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28444..29289)
                     /gene="phnJ"
                     /locus_tag="ACHXOX_001519"
     CDS             complement(28444..29289)
                     /gene="phnJ"
                     /locus_tag="ACHXOX_001519"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR7005023.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(29282..30346)
                     /gene="phnI"
                     /locus_tag="ACHXOX_001520"
     CDS             complement(29282..30346)
                     /gene="phnI"
                     /locus_tag="ACHXOX_001520"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR7005024.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(30346..30930)
                     /gene="phnH"
                     /locus_tag="ACHXOX_001521"
     CDS             complement(30346..30930)
                     /gene="phnH"
                     /locus_tag="ACHXOX_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR7005025.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30927..31379)
                     /gene="phnG"
                     /locus_tag="ACHXOX_001522"
     CDS             complement(30927..31379)
                     /gene="phnG"
                     /locus_tag="ACHXOX_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR7005026.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31380..32105)
                     /gene="phnF"
                     /locus_tag="ACHXOX_001523"
     CDS             complement(31380..32105)
                     /gene="phnF"
                     /locus_tag="ACHXOX_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR7005027.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32126..32905)
                     /gene="phnE"
                     /locus_tag="ACHXOX_001524"
     CDS             complement(32126..32905)
                     /gene="phnE"
                     /locus_tag="ACHXOX_001524"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR7005028.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(33031..34047)
                     /gene="phnD"
                     /locus_tag="ACHXOX_001525"
     CDS             complement(33031..34047)
                     /gene="phnD"
                     /locus_tag="ACHXOX_001525"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7005029.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(34072..34860)
                     /gene="phnC"
                     /locus_tag="ACHXOX_001526"
     CDS             complement(34072..34860)
                     /gene="phnC"
                     /locus_tag="ACHXOX_001526"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR7005030.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34993..35436)
                     /gene="yjdN"
                     /locus_tag="ACHXOX_001527"
                     /pseudo
     CDS             complement(34993..35436)
                     /gene="yjdN"
                     /locus_tag="ACHXOX_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(35594..35929)
                     /locus_tag="ACHXOX_001528"
     CDS             complement(35594..35929)
                     /locus_tag="ACHXOX_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR7005031.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7787 a         9717 c        10188 g         8374 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtcacc gcattcataa cttatatatt ttatttatta attggctgta tatattttta
    20881 aaataaatgc ttttgggggc ttcagcgccc ccaaaagcga gtgacattaa tttggggtta
    20941 cgatattcac ccacaaatcg aaattatcca gacaggcgat aatttcttcc agtttcgcgc
    21001 cattgccaat aattttggct ttcttcgctt ttaccagatc cgccattttg gcttgcccgg
    21061 tcagcacggc gtgcagatct tcacggctga tgtagaaaga ggcgtcagct tgggattgca
    21121 gtgttttacg gtagttaagc acgctatcgt tcagcgtcag gttgaggtta tcgccattgc
    21181 tcatattgaa gttcaggctg atatttttac ccgcggcttt cgcgctatcg aggcgaacgg
    21241 acataaagtc gaacagcatt tcgaccgaca tcccgcgaat ggtgtccggg gaaccgatgg
    21301 tgccgtggct gaacttatgc accccttcgc gcagctcttt cgcgccggtc aggtagaaac
    21361 cgcgccaggt ggcggactcg gcctgatagc ccagctgttc aaagttattc gcttgcagat
    21421 tcttcgcgac ctggtcaccc gggttggcgg caatcacctg tttcagcagt tctgccgacc
    21481 agcggtaatc accttgcttg ttcgcttctt gcgccaggtt gatgacacgg gcagaaccgc
    21541 ccagcgcctg cacgtaacgt ttacccatct ccacctgacc atacggatgc aggttagccg
    21601 ggttaccgtc gtaatagcca agatagaagt tatacaccgc gcgggcgttg tggctgacag
    21661 aaccgtaata gccacggctg gcccagttat tggcaagtgc aggcggcagt ttaatcatgt
    21721 cgccgatttc attcatggtg tagccctggt tcgccaggtg cagggtctgg tcgtgaatgt
    21781 acttgatagt atcgcgatat ttaccaatat aatcattgat atgcttattg ccccagaccg
    21841 gccaggtgtg cggcataaac aggacttccg cgtcgttacc ccacatatcc agcgtttcgt
    21901 tcagatactc ggtccacttg ctggtgtcgc gggttttcgc gccgcgcaga gtgtagaagt
    21961 tgtgcagggt atgcgtggcg ttctcggcgg tacacagcgc tttcagggcc ggaatataga
    22021 agtgcatttc ggctggtgct tcgctacctg gggtcatcag gaagtcaaac tccaggccgt
    22081 cgataatcat cttctcaccg gttttggtga tggttttgtt cggtgcgatg atgctcgggt
    22141 agcccgatgc cagcgttacg ccaagaccat tgccgatgtt accctgaggg ttatgcgcca
    22201 gcagcaggcc gtaggagtaa agtgcacggc ggctcataat attacccgcc agtacgtttt
    22261 cgctgatggc ttcgtccata aagccagcag gggcgataac ctgcacttta ccggatttca
    22321 catcagcttc agagataatg cctttcacgc cgccatagtg gtcggtatgg ctgtgggtat
    22381 aaataaccgc aacaatcggt ttttgcgggc gattttggaa gtaaaggtca agggcggctt
    22441 ttgcgctcgg tggcgtcacc agcgggtcga tgacaataat cccggtatca ccttcgataa
    22501 aggtgatgtt ggagatatcc tgaccgcgaa cctggtacat tctgtcggtg actttaaaca
    22561 ggccggaaat accgtttaac tgcgactgac gccacaggct agggttaacg gtttcgggcg
    22621 cgggcgcatt gatatcaaat ttataatctt ccccgcgata atatggtttg ccattagcat
    22681 cgcgcagaat accttcatcc agcagcggag cgataaatcc acgctgagcg tcgtcaaaat
    22741 cctgacgatc gctaaacggt aatgatttgg cgtaattctg attaacctgt tgggtatatt
    22801 gagtggcggc tttcgcttcc tctttcgcgt tagcggtatt taccatcatg ccgctggcgg
    22861 cagttaacgc aataacaatt ctgctcaaac ggaataaccg agagttattc atgtaaatct
    22921 ccatttatca tttatgtcgt aaatatgtaa tccatgcaaa atacgtcatt taaagctggg
    22981 gttatcataa ttgggaattt gcgactttaa taagtggaag tgtgagctga acgatccatt
    23041 ttattaggca tttgtgctga agagggaaaa ttacgcgaat ttattatctc tgtaaataat
    23101 atgaggtgtt ttatatattt attatatata ttgtacttcg gcaaatactg agcacaattt
    23161 atcaatgcaa ccggtgtttc tttttaaaaa acgtaccgga gcaatgggcg gtcatgtcgc
    23221 tggcaccgca ggattctgcg ttgctgtgcc agccttttat tcatggtcaa tgagtgaggt
    23281 gattgataaa gacaccaggt cgtcagagcg cactgattat ccgcattatg ccagtgaagt
    23341 tgcactggct tttggttcac gagtggttgg cctcgaactg gcaaaaatga ttgtggatgc
    23401 gtggctgggc gcacagtacg aaggcggtcg tcatcaacaa cgcgtggagg cgattacggc
    23461 aatagagcag cggagaaatt gagattcatc cactacttgc atggatgagt aatgactaat
    23521 gtggatagag tttctttttg aggttggcta atgaaacgct ttccgctatt tcttttattc
    23581 accctgctca cgctgtccac cgttccggcc caggctgata ttatcgacga tactattggt
    23641 aatattcagc aggcgattaa tgacgcttat aaccctgacc gtgggcgaga ctatgaagat
    23701 tcgcgcgacg acggctggca gcgtgaagtg agcgacgatc ggcgcagaca atatgacgac
    23761 cgccgccgcc agtttgaaga tcgccgcagg cagctggacg atcgccagcg ccagcttgat
    23821 caggagcgtc ggcaactgga ggatgaagag cggagaatgg aagatgagta tgggcgatga
    23881 ggttgggtat ggagcaggca tcgcccctca ccccaatggg gagagggaga aaacgagcgc
    23941 aatattcaat atcgagcaca atcggtcccc tcgcccctct ggggagaggg ttagggtgag
    24001 ggggccagcg cgcacattcc ctctcatcac gccaccccaa tctccatccc atcaaacccc
    24061 acctcaaacc ctgacggtag tgcgttttcc atcagccacg catcaaactg gtggctgata
    24121 tgggtcagaa tcacccgtgg cgagcggata acctgattca gcgcaagcac ggtatttaaa
    24181 tcacagtgat tacgcggtgc atccgcgcgc ggcgggtgac tgcaatccat caccattacc
    24241 tgcggctgat tattgcgtaa aaatttcagc gttttttccg gcaagccagc ggtgtcagac
    24301 agccacgcca cccggctgtg cgccgtttcc agtaaatagc cgaaagtgag ttttgagtgg
    24361 ttaagcggca gcggcgtgac ctgcaacccc tgcaaatcaa acaccacaaa cggttccacc
    24421 gtgtggctga aatcaagcag cccgggatgt ttaaacagat cgtcgcagcc ctgttcatcc
    24481 ggcgggccgt agatcgggat cggatcgccc acgccccagc gcagcggaaa cagcccctgg
    24541 acgtgatcca tatgataatg cgtcagcaaa aactgctgga acgatccggg agaccagcga
    24601 tcggtgagat cgtgcagccc ggcgtcgatc agggtgatcg cctcgttaaa cttcactacg
    24661 ccgctgcacg gttggcggcg atactgcggc gagcgccgcg ctcggtcgca ggccgcgcac
    24721 tcgcagcccc atgccggaac gccctgtgcg ccgccggtgc cggtgagcgt gagggtcaga
    24781 ctcatgttac agcgccttgg tgaagcggaa gtgactctgc tcgtagcctt ctcgcagata
    24841 gaaacggtgc gcgtcgtggc gcttcacgtt ggtcgaaagc tcggtcattt cggccccggc
    24901 ctggcgggct tcttcttctg cccacgccag caacttgcta ccgacgttca ggccgcgcgc
    24961 ctgcggcatc actaccagtt cctgaatttc gccgatccag ttgacatgat gcagatgaaa
    25021 ctgcaaatgc aggccgatca tgccgacaac ctcgccatcc agcagcgcca gatgatagcg
    25081 catgttcggg tcgcgcagat tggcgctaaa gcccacgcga aatgcgtggt ggtcaaactc
    25141 cgcctgtttt agctcacaaa tcagcgcgta aaccgcgtcg gtatcatact gcgtggccgg
    25201 gcgaagctca caagcaggca tggtgtttct ccttctgatg aatcagcgtc agcagcgtgt
    25261 cgaccgactg gcgcaggctg ccgtcattgt tgagcgtatg gcaatcctgt ggagtatagc
    25321 gggcggcgcg cgccaggcgg gcgttaattt cactggcgtt ttcacggcca cggttttcca
    25381 ggcgctggcg gaggatctcc ggcgaaacct gtaaacagac gggcagcagc gccgattgat
    25441 agcgcgcccg cgcctgcggc agatgggcgc gtgagccgtt gaccagcacg tcgaatccgg
    25501 cgtgcagcca gaggtcgatc tcgacgccga cgccataata cagaccgtta gcgtgccagc
    25561 ttaaggccag taggttctgc cccgcgcggg taaaaaactc ctgctcgctc agagcgatat
    25621 ggttttcgct tccggcgctg gcgtcgcgcg tgatgtagcg atgcgccacc agtagctgag
    25681 tttgttcccg caggcggagt tccgccagca ggctatcttt cccggagccg gacggcccca
    25741 ttaaccaaat cagttttccc atcatcagaa caccctttta ccctgacgcc agacgtggtc
    25801 gatatgaatg tgattgccct tgcgatgcgc cagcaccaag tccgcgcgtt tgccctcgcc
    25861 aatcaccccg cgatcctgga gattaagcgc ctgcgctgga tttttagtca ccagcttcac
    25921 cgcctgcggc agcgtaaagc ggttgctctc gtcatcggcg acgcgaaatg ccgcatcgag
    25981 caggctggcg gggtagtagt cggaagagag gatatccagc aggccaagct gcgccagttc
    26041 actggctgcc acgttgccgg agtgcgagcc gccgcgcaca atattcggcg cgcccatcag
    26101 cacgttcatg ccatgcttgc gcgaggcttc cgccgcttcg aacgtggtgg gaaattcggc
    26161 gatcacgctg ccaagctggt gagattcagc aacgtgggcg tgggtggcgt catcgtggct
    26221 ggcaagcgca atttttcgcg cgcggcacag ggcggcgatg gattcgcgat tcggctgcga
    26281 ccagcgtgcg gcgagcgcca gttgctcttc ttcgtactgc tgcatctgcg catcagtgag
    26341 ggagtatttg ccctgataat attcgcgata cttctcgcga ttggcgaact ggcgctggcc
    26401 cggcgagtgg tccatcagcg acaccagcgt caccggctcg cgctgcacca gtttttcaaa
    26461 cagcggcagc gtggtgtgat gcggcagttc gcagcgcaga tgcagacggt gctcggcgcg
    26521 gttgacgccg cgtttctgcg tctcttcgat ggcgttgatc atcttctcca gattcaccag
    26581 ccgatcgccg ccgtcgcgca cgtcgccaat tgccacggca tccagtacgg tggtgatgcc
    26641 gctcgccacc atcagcgcgt cgtggctgct catcgccgag tgggcaggcc agtcaacttt
    26701 cgggcgcggg gtgaagaatt tatccagatt atcggtatgc agctcaatca gccccggcag
    26761 cagccagccg ccttcgccgt ccatcgcctc cggcaggcgg ctctggcttt cggcaaaggc
    26821 gcggatttcg ccgtcctgca cctccagtga accgtgtacc acttcgtttt ccagcaccaa
    26881 tttgacgtta ttgataatca tgaagaggct tccattgggt gcaggcggtc ggcaacgtca
    26941 ttacgtacgg cttcgtcgtg gaagatgccg acgatggccg cgccacgggc tttggcttcg
    27001 tgaatcagct cgactacggc ggcgctgttt ttggcgtcca gcgaggcggt aggttcgtcg
    27061 agcagcagaa tggggtagtc gacgataaag ccgcgggcga tgttgacgcg ctgctgttcg
    27121 ccgccggaga aggtcgatgg cgccagatgc cacaggcgtt ccggcacgtt caggcgggtg
    27181 agcagacgcg cggctttagc ggcgcaggct tcacgcggta cgccggtatc gagtagcggc
    27241 tgcatcacca cttccagcgc cgaaatacgc gggatgacgc gcagaaactg gctcacccag
    27301 ccaacggtgg ttttgcggat ctccaccact ttgcgcgctg gcgcggtgac cagatcgacc
    27361 cattcgtcac cgtgtttgat ctggatttgc ccttcgtcgg gcagatagtt ggcgtacagc
    27421 gagcgtagca gggttgattt tccgctgccg gaatggccgt ggagcaccac gcattcgccc
    27481 gcgttgacga tgagcgaggc gcggttgagg acgggcaggc gcacgccgtt ttgctggtgc
    27541 aggatgaagg ttttactgac gttttgtacg ttaatcattt ttcgcctcgt ggttcgggtg
    27601 tcggatgcga cgctgacgcg tcttatccga cctacgggga gcgcatttgt aggccggata
    27661 aggcgtttac gccgcatccg gcaccggctc aattctgcaa aaccgatgac accagcagct
    27721 gtgtatacgg atgatgcggg tcgtcgagca cgcggtcggt taacccactc tccaccactt
    27781 gcccctgctt catcaccagc aaacggtccg ccagcaggcg ggcgacgcct aaatcgtggg
    27841 tgacaatcac taccgcgagg ttcagctcca ccaccagtcc gcgcagcagg tcgagcaggc
    27901 gggcctgcac cgacacatcc agcccgccgg tcggttcatc cataaacacc agcttcggat
    27961 gcgtcaccag gttgcgggca atctgcaaac gctgctgcat accgccggaa aaggtggtcg
    28021 gcaggtcgtc gatacggttg gcgggaatct ccacctcttc cagccacttc tgcgcggtag
    28081 cgcgaatatc gccgtaatgt cgcgccccgg tcgccatcag ccgctcgccg atattgccgc
    28141 ctgccgacac ctggcggcgc aggccgtcga gcggatgctg atgcaccacg ccccattcgg
    28201 tgcgcagcag gcgacggcgg tcggcctcgc tcattgcata gagggaacgg ttttcatagt
    28261 gaatctcccc ctgctgcggc gtcagacgcg cggagatcga cttcagcagc gtggtcttcc
    28321 cggagccgga ttccccgaca atgcccagca cttcccccgg ccataaatca aaagagacat
    28381 cgctaaagcc tttgcccggc gcgtaaaggt gggtcaggtt attgaccgaa agtaacggtt
    28441 gattcattgg cttttcgcct cgctctgttg gcggcaataa tcggtatcgg agcagacaaa
    28501 catgcggttt ccggcgtcat ccagcaccac ttcatcaaga tagctgtggg tcgatccgca
    28561 gatggcgcat ggctcatccc actgctgaac ggtgaacggg tgatcgtcga aatcgagact
    28621 ttccacgcgg gtaaacggcg gcaccgcata gatgcgcttc tcgcgccctg cgccgaacag
    28681 ctgcagggcg ggcatcatgt ccattttcgg gttatcgaat ttcgggatcg gcgacgggtc
    28741 catcacgtag cgcccgttca ccttcaccgg ataagcatag gtggtggcga tatgaccgaa
    28801 gcgggcgata tcttcataca gtttcacctg catcacgccg tactcctcca gcgcgtgcat
    28861 agtgcgggtc tccgtttcgc gcggctcgat aaagcgcagc ggttccggga ttggcacctg
    28921 gaagataatg atctgatctt cggtcagcgg cgtttcgggg atgcggtgac gcgtctggat
    28981 aagcgtcgca tcgtccgtac attcagtggt gtttaccccg gtcacgcgct taaagaagtt
    29041 gcgaatcgac acggcgttgg tggtgtcatc cgcaccctgg tcaatcacct tcaacacgtc
    29101 gctttcgcca atcacgctgg cggtgagctg gataccacca gttccccagc cgtacggcat
    29161 cggcatctcg cgcccgccaa acggtacctg ataaccgggg atcgccaccg cttttaagat
    29221 ggcgcggcgg atcatgcgtt tggtctgctc gtcgaggtag gcaaagttgt agccgctcag
    29281 attagccatg gttctgctcc tgttgcagac gtttgagtag ctccagttcg gcctggaaat
    29341 cgacgtagtg cgggagtttg aggtgcgaga caaagcctgc ggcttcgacg ttgtcggcat
    29401 gcgccagcac gaactcttca tcctgcgccg ggcctgtcgc gtgctcgccg tattccgggg
    29461 cttgcaacgc acggtcaacc agcgccattg ccatcgcttt gcgctcgctc atgccgaata
    29521 ccagcccgta gccgcgcgtg aagtgcggcg gctcatccgg cgggtcgata aaaccgttga
    29581 ccatttcaca ctcggtcatc agcagttcgc cgacgtttac cgcaaatccc agctcttccg
    29641 gcacaatcga cacgtcgatg taaccactgc ggatctcgcc cgcgaacggg tggttgcgcc
    29701 cgtaaccgcg ctgggtggag taagccagcg ccagcaaata gccttcgtcg ccgcgcatta
    29761 actgctgcaa acgggaagaa cgtgaacagg ggtaaaccgg cggcgtgcgg gtgatgtcat
    29821 ccggctgtgc tccgctatcc tcttcaaact tcgccagccc ctgacgcgcc agcaggctga
    29881 aaacgtgcgg cgacggctgt tgttcgctgt cggcggtggt cagcgtcggc gcttcgccgt
    29941 ttgccagcag agtgaaatcg agcaggcgat gggtgtagtc gtaggttggg ccaagcagct
    30001 ggccgccggg aatatcttta taaaccgctg aaatacgccg ttcgagacgc atctccgtgg
    30061 tgtcgagcgg ctcgcttacc gccagtttcg tcagcgtggt gcggtaggcg cgtagcagga
    30121 aaatcgcttc gacgttatcg ccgctggcct gtttcagcgc cagcgccgcc agttcgcggt
    30181 cggcaatgcc gccttctgtc atcacgcgat ccaccgcgag gttaagctgc tgttcaatct
    30241 gggcgacgct cagttcgggc aaatcggtat cgccccggcg tcggctctct tgcagggcgt
    30301 gggcggcgtc gatcgccttt tcgccccctt tcacggcaac gtacatcagc acacctccac
    30361 atgagtggtt cgcgggatag ccagcaggcg ctcgccgcag gtcaggatta ggtcgatgcc
    30421 gagcgggaac gggtgcgggc gctcggtgag ttcgtgcaga atgcactctg gcagctgcgg
    30481 agcgatcatt cgttcttcgg caatgcccgc gccggtgaga cgcaacatgc gtccgccgct
    30541 caggctggcg acctgtaaaa tcagcgtcgc gcccgcttcc ggcgcaacgg cggtgtcggt
    30601 ggaaagggcg ttgagctgtt cgctggaaat cgcctcatcc gtcaccgcga aggtcgcctg
    30661 ttccggctgg ctgaccagcg gcgcgttggt atgaaaacgc aggctctgat tgacgatatc
    30721 gttacttaat ggcgtggcaa gccacaccgg cgtgtcgtta tcggccagcg tcagcagcac
    30781 gctggtggtg gcgatattca gcggctgcca gccgcgtttg agctgataca gggcgacaat
    30841 cacgcccggc tcgctcatgg cctttaacag gcgacgaaaa ctgtgctggg catcctgcac
    30901 gggaagcata aaagcggttt ccagggtcat gcgttgtctc cgcgaaccat cgtaaagaag
    30961 tcgacccggc tggcgttcac ttcggcctgg cgtgcggcaa tgcgtgccat acggtcagcg
    31021 tccagcgggg caataagggt ttctgataag ttttgaaagt ggcgagattg ctgcatcagc
    31081 gcatcaatca gcgcgcagcg ttcggcgtgc tgtttatcac gccccagcac ccagctgtag
    31141 ccgagggtgc cgtcagtcag gcgtacggcg gcacgggtca gcgtggcgtc gccggcaaaa
    31201 aagcgttcgc cggtaccgcc cattcgcgcc tgaatctgca ccaggccagt ttcggcagtg
    31261 cggatcactt catagtcggc ggtgatgttc agcgcgttca ggcgggctgc cagttcagcc
    31321 ggttggctgt gcgccagtac ggacatccag tgctggcggg tcgcggtatc tgcgtgcatt
    31381 cagtgctcca tagtgaattc aatcatgtcg gcgcgcgtca ggctgacgga gtactccgcc
    31441 gggctgcttt caccgtcacg gtggttaagg gtgcgcacgc acagcagcgg cgacatattc
    31501 gggatttcaa ggcgctggca ctctttggcc tgggcgcggc gggcgctgat ccgcgtctgg
    31561 ctgcggcgca gcgcaattcc ggtttgctcg cgcagaaaat cgtgcagcga accgctgtcg
    31621 aaacgttgca gtgtcggcca gagggtgagg tccgcaaagt agtggtcgat taaacagagc
    31681 gcgacgccgt tgacgcgacg cagggtgcgc aaatggataa cgttctcacc ctcggtaatc
    31741 cccagcgcgt cagcaacgtg accggacgcg gggcgcaata ccgacagcag tttttcgctg
    31801 gtgggatggc tgccctggtc cagcaggttc tggctaaaac gcgcctgggc gttgagcggg
    31861 taatcgaacg ggcgcatcag caccagcacg ccgatgccct gacggcgctg tacccagcct
    31921 ttttccacca gttggtcgat ggcgcggcgc agggtgtggc ggtttacctc aaagcgcgcc
    31981 gccagttgct gctcggcggg aagatagtcg ccgcagcgat agtgttgacg aagctcctgc
    32041 tcaagttttg cggctatctc ctgatagcgt gttgggtagc tggtcggatg tgtagacaag
    32101 tgcatagata tcaatgcctc acttatcaga taaagtgctt acgcaaccgt tgagagagga
    32161 aatccagcag gctgaccgtg acgatgataa gcaccatcag ggcgcaggtt tgttggaact
    32221 ggaaaccgcg aatcgcttcc cacagggtga cgccgatccc gcctgcaccg accatgccga
    32281 cgacggtcgc cgagcggacg ttggattcga agcgatagag ggagtaggag atcagcagcg
    32341 gcattacctg tggcagcacg ccgtaaagga tctcttccag cttgttggcg ccggtggcgc
    32401 gaatgccttc caccgggccg ggttcaatcg cttccaccgc ttcggaaagc agtttcgaga
    32461 gcacgccggt ggtgtgaata aatagcgcca gcacgccagc gaacggtccg agaccgacgg
    32521 cgaccacgaa cagcatggcg aagaccattt cgttaatggc gcggcaggcg tccatcaggc
    32581 ggcgaacggg ctggtaaacc caccacggca ccaggttttc ggcgctcatc aggccgaagg
    32641 ggatggagag aaccactgcc agcgcggtgc cccagacggc gatttgcagc gtgacggcca
    32701 tttcggtgag gtaatcctgc cactggctga aatcgggcgg gaagaagtcg gcggcgaagg
    32761 tcgccatgtt gccgccgtct ttgatcagcg taagcggggc catttccgcg ccctgccacg
    32821 agacgaccaa cacggcgaga acaatggccc agctcagaag cgagaaccag ctgcgcttgg
    32881 gtggggcgat ggtgatggtt tgcatgtttg gctccgttta atgttttctc cctctccctg
    32941 tgggagaggg ccggggtgag ggcatcagcg tgcgcatttc cccctcaccc taaccctctc
    33001 cctcaaggga gaggggaccg atcgagcaca ttactgcacc gctttactca ccgaactcat
    33061 cgcgcttaac gcgttgttca ggcggtccag gtcatccagc tgcgcctgaa tctcggtggt
    33121 tttcgccagc ttgtcctgct cattcagtcc gttattgctt tttacgccct gaatctcttt
    33181 aaacagtgcg agctggcgaa tcggcaccag ttgcaggtcg ctggaggcgc gaaacggtgc
    33241 ccagcccagg cgttccagca ccgctttctc ttccggcgtt tttccgtagt tcataaagaa
    33301 gtcgtagatc ttgtctttgg tggtttcgga gagattcttg cgccagacga tcggatcgcc
    33361 tgggatcagc ggcgacttcc agatcacctt cagttctttc agcttctctg gcgcggaggt
    33421 tttcagcttg tcgaggtttt cggtgttgtt ggtggcaaca tccacctgct tgttggcgac
    33481 ggccagcgcg ttggtttcat gcccggcgtt gacggtgcgc ttaaagtcgc tggcggagat
    33541 attgtttttg gcaaagacgt agtaaccggg gacgaggaag ccagaggtgg agttaggatc
    33601 gccattgccg aaggtgagat ctttccgctt cgccagcaga tcgttcaggt tgttgatcgg
    33661 actatctttg ttgacgatca acacgctcca gtaacctggc gatccatccg ccgcgaccgt
    33721 ctgggcgaag acctggccgt tggcgcgatc caccgcttcc attgccgaca ggttgccgta
    33781 ccaggcgata tccactttat tgaagcgcat tccctggata atgcctgcgt agtctggggc
    33841 aaagaaggcg ttcaccttca cgcccagctt cttctccata tcctgtaaga atggcgtcca
    33901 ttgcggtttc aggttttgct gtgattccgt tgaaataatg ccgaaattca gcgccttttc
    33961 ctgctcttcg gcgtgtgccg ggcttaacag ggtgctgagg ctgaacatgc tggtgaaggc
    34021 cagcgaggca attatcttag cgttcattcg ttttcctcaa tgatggggat gtcaggcagc
    34081 tttcgcgttc tcttcgatgc gattaatgct gcggtagaga tggtcaaaac gttcgttatc
    34141 aaactgttgg ctgctgccgt cgtagaaaac gtgcccctgg cgcagggcga cgatgcgttc
    34201 gcagtagcgc agggcgtaat ccacctgatg cagcgtgacg accacggtga tgccgtcgtt
    34261 ctgattgatg tcgcgcaggg tgtccatcac gatgccggcg gattccgggt ccagcgaggc
    34321 gatgggttca tcggccagaa tcaccttcgc ctgctgcatc agcgcgcggg caatcgccac
    34381 acgctgctgc tgtccgccgg agagggtgga aacgcgttga tgggcaaaat gcaccatgcc
    34441 aacgcgggtc agcgcctgta acgcgcgttg tttctgctcg cgggtaaacc agctaaaaca
    34501 ggtgcgccag aacggcgtgc tgccgagtgc gccaatcagc acgttctcca gtacgctcag
    34561 gcggttcacc aggttgaatt gttggaagat gtagccggtg ttggcgcggc ttttgcggat
    34621 atcgcgcgcc agacggcctt cgcgctggac tgtgcggccc agcagctcga tatggctgcc
    34681 ggcggattta tcgccggtaa tcaaaccgct taagtgacgt aaaagggtgg atttgccgga
    34741 acccgacggc ccaagcagag ccaccatttc accgtgatga atgttcagat caaccgcatg
    34801 cagcgcctga tgctgattga aggttttggc gagcttctcg acacggataa tcgtttgcat
    34861 gatgcagcct ccctaaaaaa gtggctccat cgtggcggat tattgtgacg atttggttaa
    34921 ttaaaagtga ctaacagatg aagagttaac gggaattcga tgacagtgcg ggagggcggg
    34981 cctcccggcg ggttattgcg ttggttgttg tttgacgaca ttaatcatcc acggtacgcc
    35041 aaatttatcg gtgactttgc caaagccatg cgcctagaaa gtttcctgcc aggccatttc
    35101 gatttttccg ttagcggcaa gattgtcaaa ccagcgtttt ccttcttcga cctgttgcga
    35161 atcgagcacc agcgtaaagc cggagtagct ggcttttcct gacggcatgg catcgctcat
    35221 catgatgtcg cttccggcaa tgcgcacatt ggcgtgggcg atggcggtat cgggaaattg
    35281 cattccggaa gggcagttct cggcgctgtc ctgcgctgat tttggcattt cgccgaagct
    35341 gattttatag agcagttcag cgcccaacgt acgttgataa taagcaattg cgtcggcaca
    35401 gttaccggca aaagagaggt agggacttaa cggcatgatg gtgacctcag ttaagagaag
    35461 ccaattaagt gtagttcgaa atttatacag atgagaggct gcctgataag acgcgcaagc
    35521 gtcgcatcag gcagtcggca tcgttgccgg atgcggcgcg agcgccttat ccggcttacc
    35581 gatcacaata caatcagttc tttttcacaa actcagattt cagtttcatc ggaccaaaac
    35641 cgtcgatttt gcaatcgatg ttatggtcgc cttcaaccag gcggatgttt ttcactttgg
    35701 tgccaatttt cagcatcgaa gagctacctt tcaccttcag atctttaatg atggtaacgc
    35761 tgtcgccgtc agccagcaga ttgccgttag catctttaac gatcagctcg tcgctttcct
    35821 gtgcaggttc tgcgtcgttc cattcgtagg cacattccgg gcagatgtac atgccgttat
    35881 cttcgtaagt gtattcggag ttgcattttg ggcagtgtgg taatgacatg tggatttcct
    35941 caaaagtcag cagggcaaaa gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta
    36001 tacacaaaat ccctgagttt gtcgggggta ctgctgattt tagacgacga tcgttttcat
    36061 ttgaaa
//
LOCUS       DAZRVY010000030        35410 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZRVY010000030 DAZRVY010000000
VERSION     DAZRVY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.030"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..212)
                     /locus_tag="ACHXOX_001529"
                     /pseudo
     CDS             complement(1..212)
                     /locus_tag="ACHXOX_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(441..1361)
                     /gene="malM"
                     /locus_tag="ACHXOX_001530"
     CDS             complement(441..1361)
                     /gene="malM"
                     /locus_tag="ACHXOX_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR7005032.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1513..2853)
                     /gene="lamB"
                     /locus_tag="ACHXOX_001531"
     CDS             complement(1513..2853)
                     /gene="lamB"
                     /locus_tag="ACHXOX_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR7005033.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2925..4040)
                     /gene="malK"
                     /locus_tag="ACHXOX_001532"
     CDS             complement(2925..4040)
                     /gene="malK"
                     /locus_tag="ACHXOX_001532"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR7005034.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4405..5595
                     /gene="malE"
                     /locus_tag="ACHXOX_001533"
     CDS             4405..5595
                     /gene="malE"
                     /locus_tag="ACHXOX_001533"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR7005035.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5749..7293
                     /gene="malF"
                     /locus_tag="ACHXOX_001534"
     CDS             5749..7293
                     /gene="malF"
                     /locus_tag="ACHXOX_001534"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR7005036.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7308..8198
                     /gene="malG"
                     /locus_tag="ACHXOX_001535"
     CDS             7308..8198
                     /gene="malG"
                     /locus_tag="ACHXOX_001535"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR7005037.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8570..10045
                     /gene="xylE"
                     /locus_tag="ACHXOX_001536"
     CDS             8570..10045
                     /gene="xylE"
                     /locus_tag="ACHXOX_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR7005038.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10089..10499)
                     /gene="psiE"
                     /locus_tag="ACHXOX_001537"
     CDS             complement(10089..10499)
                     /gene="psiE"
                     /locus_tag="ACHXOX_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR7005039.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10625..10768)
                     /locus_tag="ACHXOX_001538"
     CDS             complement(10625..10768)
                     /locus_tag="ACHXOX_001538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005040.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10769..11047
                     /locus_tag="ACHXOX_001539"
     CDS             10769..11047
                     /locus_tag="ACHXOX_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005041.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11094..13190)
                     /locus_tag="ACHXOX_001540"
     CDS             complement(11094..13190)
                     /locus_tag="ACHXOX_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR7005042.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13190..13927)
                     /locus_tag="ACHXOX_001541"
     CDS             complement(13190..13927)
                     /locus_tag="ACHXOX_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR7005043.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13924..14562)
                     /locus_tag="ACHXOX_001542"
     CDS             complement(13924..14562)
                     /locus_tag="ACHXOX_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR7005044.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14676..14918)
                     /gene="yjbE"
                     /locus_tag="ACHXOX_001543"
     CDS             complement(14676..14918)
                     /gene="yjbE"
                     /locus_tag="ACHXOX_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR7005045.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15380..17029)
                     /gene="pgi"
                     /locus_tag="ACHXOX_001544"
     CDS             complement(15380..17029)
                     /gene="pgi"
                     /locus_tag="ACHXOX_001544"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR7005046.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17266..17462
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17554..18903
                     /gene="lysC"
                     /locus_tag="ACHXOX_001545"
     CDS             17554..18903
                     /gene="lysC"
                     /locus_tag="ACHXOX_001545"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR7005047.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19155..19427
                     /locus_tag="ACHXOX_001546"
     CDS             19155..19427
                     /locus_tag="ACHXOX_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR7005048.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19560..20432)
                     /gene="rluF"
                     /locus_tag="ACHXOX_001547"
     CDS             complement(19560..20432)
                     /gene="rluF"
                     /locus_tag="ACHXOX_001547"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR7005049.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20780..21617
                     /locus_tag="ACHXOX_001548"
                     /pseudo
     CDS             20780..21617
                     /locus_tag="ACHXOX_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            21687..22493
                     /locus_tag="ACHXOX_001549"
     CDS             21687..22493
                     /locus_tag="ACHXOX_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7005050.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22503..22910
                     /locus_tag="ACHXOX_001550"
     CDS             22503..22910
                     /locus_tag="ACHXOX_001550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR7005051.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            22910..23404
                     /locus_tag="ACHXOX_001551"
                     /pseudo
     CDS             22910..23404
                     /locus_tag="ACHXOX_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23470..24267
                     /locus_tag="ACHXOX_001552"
     CDS             23470..24267
                     /locus_tag="ACHXOX_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR7005052.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24278..25102
                     /locus_tag="ACHXOX_001553"
     CDS             24278..25102
                     /locus_tag="ACHXOX_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR7005053.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25154..26382
                     /gene="sorE"
                     /locus_tag="ACHXOX_001554"
                     /pseudo
     CDS             25154..26382
                     /gene="sorE"
                     /locus_tag="ACHXOX_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26765..27454
                     /gene="pepE"
                     /locus_tag="ACHXOX_001555"
     CDS             26765..27454
                     /gene="pepE"
                     /locus_tag="ACHXOX_001555"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR7005054.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27545..29176)
                     /locus_tag="ACHXOX_001556"
     CDS             complement(27545..29176)
                     /locus_tag="ACHXOX_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR7005055.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29396..33079)
                     /gene="metH"
                     /locus_tag="ACHXOX_001557"
     CDS             complement(29396..33079)
                     /gene="metH"
                     /locus_tag="ACHXOX_001557"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR7005056.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33279..34103
                     /gene="iclR"
                     /locus_tag="ACHXOX_001558"
     CDS             33279..34103
                     /gene="iclR"
                     /locus_tag="ACHXOX_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR7005057.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34420..35410
                     /gene="espL4"
                     /locus_tag="ACHXOX_001559"
                     /pseudo
     CDS             34420..35410
                     /gene="espL4"
                     /locus_tag="ACHXOX_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8464 a         9162 c         9057 g         8727 t
ORIGIN      
        1 gacttcacgt aacatattta ttgcatgatt ttttcgttcc gttgttttat atttaaaaat
       61 atatcactga tataaataaa gttatctctg tttaaacctg tcaccccatt gaaattacgt
      121 agtaaatcaa gatctactat attatttcct tgaaccacat catgtttcat caaagagtta
      181 ttgccgccag aatttgtgat atgacctatc ataaaacctc tgtaatacag aaataaaata
      241 acctggaaaa gtagcctgag cgtccagaca ctaccggcga aaggaaaaga atctctgata
      301 aggcattgag ataatggaca ttcttagtga agaagaagtg aagtactata aaaaatcgct
      361 catatcccag gaaggaagca taaaaaaagg tgcgccagga gacgcaccag ttgttgcaaa
      421 atcagcactg tggggcgtaa ttaccccttg ccttttacac tgctgataaa ggtggaacgg
      481 gcagatgtcg atcccaggcg ttcagcttca tcaagcagtt ttaacgcctt atcaacatca
      541 cctttcgcga cagcgttttt gatcgcggta ttaaaataac tttccgtgtc gttgagcatc
      601 ggctcgcttt tcttcaccgg caccggagca ggtgcagcca cagctggtgc cgccgtgttg
      661 cctaccgtaa ccggagctgg agcggaagaa ccaaatagtg gtcctaccaa cacgctggag
      721 ctggagttcg ttttgacttt cagtttcagt aagccatcgc tggtatgacg agcaaccgga
      781 tcgggaatat ccgggatcga gttaccgacg cctttggcat aggctttagc cgggtcgagc
      841 agttgggtcg tctgctggag atctttttcc gtggtaaaga ccagaacata aagtttttgc
      901 tgccccaacg ccggtgtcag gcgcataacg ccttccagcc gatctgcact catcacgcct
      961 ggttcctggt aggtgaaata actgctgggg aagaaggctg atggggtcat gttctgatca
     1021 agaatcagca cgttcggcgc aaaaacgctg gtttgtttgt tcacttcgct ggtcagcgtc
     1081 agggtcagtt cgccaatgtt tgccgggacg ctgtacgcag caaccggacc actgatgccg
     1141 ggaacgttca gttgttggcc gccggtcgcc agttgggtgg tctgggtttt agattgatcg
     1201 accggtgtcc aggtgagttg ttgcagcgcg gcagatggaa tggctggcgc gtcgctggtg
     1261 ttttgcggta cgtagttaac atcggcaagg ctaattccag gcgcgcttgc cagtaaccct
     1321 gctgataaac agagggcgat gagactttta ttcattttca ttgttttcac ctcaaaatct
     1381 ggagctcagc ggtagccagg caatagcgcg ctaaacccga taatcagagg ggctttcgcc
     1441 cctttcagat aatgacaacc tgtttttatg ccggatgcgg cgtaaacgcc ttatccggcc
     1501 caggttttgc tattaccacc agatttccat ctgggcaccg aaggtccact cgtcgctgtc
     1561 gccacgaccg aagctgccgc cgttgaaatc agcaggaacg gctttgccgt agttcgggtt
     1621 aaccttgcta tcgccgttgt agtcgtaacc ccatttctca tcccacttgg cgtaggttgc
     1681 gaagacacga atagccgggc gtgaccagat gctgtcgcca gcctgccatt gttgtgcgag
     1741 ggtaattttg tactgattgt tcttgtcgcc ggtgcgctgg gattcgacgt tgtcgtagcc
     1801 gatttccatc acggtgctca tgattggcgt ccacttgtac atcgggcgaa taccgacggt
     1861 ccaccacttg gtgccgttgt cgttatccca gttgatatcc tggtacatac ccacgtacat
     1921 catgtcccag ttgtcgccca tggagatcgc accgtggtcg aggatacgca gcatgtgacc
     1981 gttgttgttg atattgtagg caaatttttc gttatcaaac gcgacgccag aaccctgcga
     2041 cagaccttta ccctgcgagg tcatcgagtc agtagcgtac tgaacaacaa acttgttaaa
     2101 gcccttcagg acactctgag tatgttcagc agtgaataac cagccgtctt tcgatgcgcc
     2161 atcaaccaga cgatagttat cacgcaggtt ggcacgaccg tagtcgacac ccagttctaa
     2221 tgtgccgccc gggttgattt ccatctgcgc taaacgcaca tcgaaaacgt cgttcgcggt
     2281 ttcgttggta tagtcataaa tattgttgct ggcaaaagag gaagaaccac cagcttcaga
     2341 ggagcgggtt gctgccagag agagtttacc gaagccaaca tcgatgtttt ccagaccggc
     2401 accaggacca gaaatatccc agtagtagaa gtcgatcata tgaacgtcat gacgttggta
     2461 gaagcgctta cctgcccaga tggtggaacc tggcagccat tcgatcaggt ttttaccctg
     2521 cacgtttgct tcacggaagg ccggatcggt agcttcccag tcattctgtt gcgcgacgga
     2581 ataggccacg ttagtgtcga aatagaagct cttatcgccc tctttccaca cttcctgacc
     2641 caattttaat tcagcataag tttcacattc gttgccaaga cggtatttac tttgagcacc
     2701 ggtagtctgg aaacactgtt gttcaccgcc gctacctgtc cagccaatac cggaacgtgc
     2761 atagccgtgg aaatcaacag ccattgcctg agcagacatt acgcccgctg cgacggcaac
     2821 cgccagagga agtttgcgca gagtaatcat cattctatct cctgagtcat tgcttttctt
     2881 ttttcacatc acctgtgaca ggctttgtgt gttttgtggg gtgcttaaac gcccggctcc
     2941 ttatgcagtc gacgacatgc agtgccatcc tcacggaaca gatggcaacg ctctggcggc
     3001 aggccgatag cgaatgtggc accttcttct accaacacca cgtcgttctg gcggtacacc
     3061 aggttttgac gaatggaagg gatctggata tggatttggg tttcgttgcc gagttgctcg
     3121 acgacctgaa cttcaccctc aaggatgacg tcagcgatat cactcggcag tagatgttcc
     3181 gggcgaatac ccagcgacat attggctcca acctggacat cacggctttc aactggcagc
     3241 cagacttgct gacgatttgg catcggcagc tccacctgca cttgatcgat tgcggtggcg
     3301 gtcactttta ccggcaggaa gttcatcttt ggcgaaccga taaatccggc gacaaaacgg
     3361 tctgccggat agtggtacag ctccagcggt ttcccaacct gcgccacgcg accggcgtcc
     3421 agcaccacga ttttgtcggc cagcgtcatc gcttcgacct gatcgtgggt gacgtaaatc
     3481 attgtgcggc ccaggcgttt atgcagacgg gagatttcga tacgcatttg cacacgcagt
     3541 gcagcatcga ggttggagag cggttcatcg agcaaaaata cgcttggctc ggccaccagc
     3601 gtacggccaa tcgccacacg ctgacgctga ccaccggaga gcgctttcgg tttgcgatcc
     3661 agcaaatgcg ccagttgcag cacttccgcc acctggttga cgcgttggtt aatcacctct
     3721 ttttttgcgc cagccagttt caggccaaat gacatgtttt ctgctactga caggtgggga
     3781 tagagcgcgt aagactgaaa caccatacca acgccgcgtt ctgctggcgg agtgtcattc
     3841 atccgtttct caccgatgaa caggtcgccg ctggtgatcg tctcaagccc ggcaatcatg
     3901 cgcagtaaag tcgatttacc gcagccagac ggtccgacaa acaccacgaa ttcaccttca
     3961 tggatatcga gattgatatc tttcgatacc acgacctcgc cccaggcttt cgttacattt
     4021 tgcagctgta cgctcgccat gcccttctcc ctttgtaaca acctgtcatc gacagcaaca
     4081 ttcatgatgg gctgactatg cgtcatcagg agatggctta aatcctccac cccctggctt
     4141 ttttatgggg gaggaggcgg gaggatgaga acacggcttc tgtgaactaa accgaggtca
     4201 tgtaaggaat ttcgtgatgt tgcttgcaaa aatcgtggcg attttatgtg cgcatctcca
     4261 cattaccgcc aattctgtaa cagagatcac acaaagcaac ggtggggcgt aggggcaagg
     4321 aggatggaaa gaggttgccg tataaagaaa ctagagtccg tttaggtgtt ttcacgagca
     4381 cttcaccaac aaggaccata gattatgaaa ataaaaacag gtgcacgcat cctcgcatta
     4441 tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga agaaggtaaa
     4501 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa
     4561 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa
     4621 ttccctcagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc gcacgaccgt
     4681 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag
     4741 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac
     4801 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccacca
     4861 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg
     4921 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt
     4981 tatgcgttca agtatgaaaa cggcaagtac gatattaaag acgtggccgt ggataacgct
     5041 ggcgcgaaag cgggtctgac cttcctggtt gaccttatta aaaacaaaca catgaatgca
     5101 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc
     5161 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta
     5221 ctgccgacct tcaagggtca accatctaaa ccgttcgttg gcgtgctgag cgccggtatt
     5281 aacgccgcca gtccgaacaa agagctggcg aaagagttcc tcgaaaacta tctgctgact
     5341 gacgaaggtc tggaagcggt caataaagac aaaccgctgg gtgcagtggc gctgaagtct
     5401 tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa cgcccagaaa
     5461 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg
     5521 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact
     5581 cgtatcacca agtaatgctg tgaaatgccg gatgcggcgt gaacgccttg tccggcctac
     5641 aaaactgaaa cgcatgtagg cctgataaga cgcatcagtg tcgcatcagg cagttgttgt
     5701 cggataaggc gtgaaagcct tatccgtcct ggaatgagga agaaccccat ggatgtcatt
     5761 aaaaagaaac attggtggca aagcgacgcg ctgaaatggt cagtgctagg tctgctcggc
     5821 ctgctggtgg gttaccttgt tgttttaatg tacgcacaag gggaatacct gttcgccatt
     5881 accacgctga tattgagttc agcggggctg tatattttcg ccaatcgtaa agcctacgcc
     5941 tggcgctatg tttacccggg aatggctgga atgggattat tcgtcctctt ccctctggtc
     6001 tgcaccatcg ccattgcctt caccaactac agcagcacta accagctgac ttttgaatgt
     6061 gcgcaggaag tgttgttaga tcgctcctgg caagcaggca aaacctataa ctttggtctt
     6121 tacccggcgg gcgatgagtg gcaactggcg ctcagtgacg gcgaaatcgg caaaaattac
     6181 ctctccgacg cgtttaaatt tggcggcgaa caaaaactgc aactgaaaga aacgaccgcc
     6241 cagcccgaag gcgaacgcgc gaatctacgc gtgattaccc agaatcgtca ggcgctgagt
     6301 gacattaccg ccattctgcc ggatggcaac aaagtgatga tgagctccct gcgccagttt
     6361 tctggcacgc agccgctcta cacactcgac ggtgacggca cgttgacgaa taatcagagc
     6421 ggcgtgaaat atcgtccgaa taaccaaatt ggcttttacc agtccattac cgccgacggc
     6481 aactggggtg atgaaaagct aagccccggt tacaccgtga ctactggctg gaaaaacttt
     6541 acccgcgtct tcaccgacga aggcattcag aaaccgttcc tcgccatttt cgtctggacc
     6601 gtggtgttct cgctgatcac tgtcttttta acggtggcgg tcggcatggt tctggcatgt
     6661 ctggtgcagt gggaagcatt gcgcggcaaa gcggtctatc gcgttctgct gattctgccc
     6721 tacgcggtgc catcattcat ttcaatcttg attttcaaag ggttgtttaa ccagagcttc
     6781 ggtgaaatca acatgatgtt gagcgcgctg tttggcgtga agcccgcctg gttcagcgat
     6841 ccgaccaccg cccgcacgat gctaattatc gtcaatacct ggctgggtta tccgtacatg
     6901 atgatcctct gcatgggctt gctgaaagcg attccggacg atttgtatga agcctcagca
     6961 atggatggcg caggtccgtt ccagaacttc tttaagatta cgctgccgct gctgattaaa
     7021 ccgctgacgc cgctgatgat cgccagcttc gcctttaact ttaacaactt cgtgctgatt
     7081 caactgttaa ccaacggcgg cccggatcgt cttggcacga ccacgccagc cggttatacc
     7141 gacctgcttg ttaactacac ctaccgtatc gcttttgaag gcggcggggg tcaggacttc
     7201 ggtctggctg cagcaattgc cacgctgctc ttcctgctgg tgggtgcgct ggcgatagtg
     7261 aacctgaaag ccacgcgaat gaagtttgat taagggagat aacaaaaatg gcaatggtcc
     7321 aaccgaaatc gcaaaaagca cgtttgttta ttactcacct gctactgcta ctttttatcg
     7381 cggcgattat gttcccgctg ctgatggtcg tcgctatctc actgcgccag gggaactttg
     7441 cgatcggcag cctgatcccg gagcaaatct cctgggatca ctggaaactg gctttaggtt
     7501 ttagcgtgga acaggctgat ggtcgcatta cgccaccgcc attcccggta ctgctgtggc
     7561 tatggaactc ggtaaaggtc gccgggattt ccgcgattgg cattgtcgcg ctctctacca
     7621 cctgcgccta cgctttcgcc cgtatgcgct ttccaggcaa agcgacgctg ctgaaaggaa
     7681 tgctgatttt ccagatgttc ccggcagtac tttcactggt cgcgttgtat gcgttgtttg
     7741 atcgtctggg tgagtacatt ccattcattg gcctgaatac tcacggcggc gtaattttcg
     7801 cgtatctggg tgggattgcg ctgcatgtct ggaccatcaa aggctatttc gaaaccatcg
     7861 atagttcgct ggaagaagcg gctgcgctgg atggtgcgac accgtggcag gccttccgcc
     7921 ttgtcctgtt gccgctgtca gtaccgattc tggcggtggt attcatcctg tcgtttatcg
     7981 ctgccattac tgaagttccg gtcgcgtcgc tgttactgcg tgacgtaaac agctacaccc
     8041 tggccgtggg gatgcagcaa tacctcaacc cgcaaaacta cctgtggggt gactttgccg
     8101 ccgctgccgt gatgtctgca ttaccgatca ccatcgtctt cttgctggct caacgctggc
     8161 tggtcaacgg cctgacggca ggtggtgtga aaggttaaag atgttgttct gccaatgtta
     8221 tgccgctgca ccctcaactt acgttatccc aacttgtgac tgttattcgg cgctccacgg
     8281 agcgcctttt tttctttcgt ctgcaatctg aatcgttcgc cggttaatat ttccatcata
     8341 gagcttatta tttttacgtt atttgttttc ccacttacga taattctctt tcgtgctctg
     8401 agtcacggca atagtattgt tttcatcaat ttttgataat tatcacaatt aagatcacag
     8461 aaaagacatt acgtaaacgc attgtaaaaa atgataattg ccttaactgc ctgacaattc
     8521 caacatcaat gcactgataa aagatcagaa tggtctaagg caggtctgaa tgaataccca
     8581 gtataattcc agttatatat tttcgattac cttagtcgct acattaggtg gtttattatt
     8641 tggctacgac accgccgtta tttccggtac tgttgagtca ctcaataccg tctttgttgc
     8701 tccacaaaac ttaagtgaat ccgctgccaa ctccctgtta ggattttgcg tagccagcgc
     8761 tctgattggt tgcatcatcg gcggtgccct cggtggttat tgcagtaacc gcttcggccg
     8821 tcgtgattca cttaagattg ctgctgtcct gttttttatt tctggtgtag gttctgcctg
     8881 gccagaactt ggttttacct ctataaaccc ggacaacaca gtacctattt atctggcagg
     8941 ttatgtcccg gaatttgtta tttatcgtat tattggcggt attggcgttg gtttagcctc
     9001 aatgctttca ccaatgtata ttgcggaact ggctccagct catattcgcg gaaaactggt
     9061 ttcatttaac cagtttgcga ttattttcgg gcaactttta gtttactgcg taaactattt
     9121 tattgcccgt tccggtgatg ccagctggct gaatactgac ggctggcgtt atatgtttgc
     9181 ctcggaatgt atccctgcac tgctgttctt aatgctgctg tataccgtgc cagaaagtcc
     9241 tcgctggctg atgtcacgcg gcaagcaaga acaggcggaa agtatcctgc gcaaaattat
     9301 gggcaacacg cttgcaactc aggcagtaca ggaaattaaa cactccctgg atcatggccg
     9361 caaaaccggt ggtcgtctgc tgatgtttgg cgtgggcgtg attgtcatcg gcgttatgct
     9421 ctccatcttc cagcaatttg tcggcatcaa tgtggtgctg tactacgcgc cggaagtgtt
     9481 caaaacgctg ggggccagca cggatatcgc gctgttgcag accattattg tcggagttat
     9541 caacctcacc ttcaccgttc tggcaattat gacggtggat aaatttggtc gtaagccact
     9601 gcaaattatc ggcgcactcg gaatggcaat cggtatgttt agcctcggta ccgcgtttta
     9661 cactcaggca ccgggtattg tggcgctact gtcgatgctg ttctatgttg ctgcctttgc
     9721 catgtcctgg ggtccggtat gctgggtact gctgtcggaa atcttcccga atgctattcg
     9781 tggtaaagcg ctggcaatcg cggtggcggc ccagtggctg gcgaactact tcgtctcctg
     9841 gaccttcccg atgatggaca aaaactcctg gctggtggcc catttccaca acggtttctc
     9901 ctactggatt tacgggtgta tgggcgttct ggcagcacta tttatgtgga aatttgtccc
     9961 ggaaaccaaa ggtaaaaccc ttgaggagct ggaagccctc tgggaaccgg aaacgaagaa
    10021 aacacaacaa actgctacgc tgtaatcttc ctgtccagca cgccgcgcca gttcggcgtg
    10081 ctttcttttt actcccgctt cagccgtttc gaattacaca gccacagagt gatcaccagt
    10141 aacaggatcg cagccgagta gatcaacaca tccagtggcg atttatgatc gacgatgatc
    10201 aagcgcacaa tggcggtgat cccaatatag acaaagtaac gtagcgggaa gtgaaaaccg
    10261 gactgaaagt acttcacaat cagcgcgata aattcgaaat agagaaagta aaccaccagt
    10321 ccttctacca gctcatattt gctggtttgt tctggcgcga acagcacatc tgccagatgc
    10381 accgtttctt tgccgaggaa gacaaccaaa atcaggccga ggcacagcag gccaagattg
    10441 agtacggtct gcaaaatggt ggagataaac tccacgcgcg gacgagagag tgacgtcata
    10501 gcagtacctc cttcacagtg gcgagcttcc tgtataacgc aaaaatgtga cggagatcta
    10561 tattttttgt gtatgttttg ttcaacctgg atttggagcg cgcctccacc aaagccggat
    10621 atacctaccc gtaggccgga taagccgcgc ctggcggcgc atccggcaag acaaaccgca
    10681 cccaagccgg taattcaaaa atcacccatt caggtgattc ccagccacca gcaatgcgcg
    10741 aaaaatcgac aatgttcctc tggaacaaat gaaacgtaac ctgataaagg tggtaaaaat
    10801 gaagccttat tttgctgctt tgatgttatc agtctctgtt cttcctgctt atgctggtcc
    10861 gctcggtact gctgataaag ccgacctacc gcaaagcaat gtctccagcc caatgatggc
    10921 ccagtcgctc aggcaaccag acctgcaacc gatatcgacg gacaggaaga cagaatgttt
    10981 tcgcctttac acaccagatc gcaaaccggg ggtgaactgc gttcctgatg gctcaacagg
    11041 ccattaagaa tgagggtgcc agtgattgcg aaaactggca cctttcccat gacttagcgg
    11101 aaattgacgc tacggtcgct ggtcatatca tacaactgga acttacgccc aagttgctga
    11161 ccaccgtcac gcgtcagcgg cgtccagcca atcgccgcac ggctgcgtgt cgggccagac
    11221 gagaagagat ccaacggtac cgagacatac acgcctttgg tgaagtcccc ttcgccgtac
    11281 tcctcttttg aaacattcgt gatcgtggca tagccaccca ccaccacgcc gctatcaaag
    11341 cgtttggcga tctccagcgt gccgccttta tcccctgcca gatactgccc gacgctggct
    11401 ttcactaaca cgtcctgggc gaaagatggc gtccagtagg cggtcagatg tccggttttc
    11461 acgctgtagt cggtgaattt catcatatct tttgcactac gccagtcgcg ctgtttaacg
    11521 tagttggcat ccagaccaaa cgcccagttg ctgtccagcg gacgatacaa cacttcagcc
    11581 cccgcaccgc caaacatggt ttcgagataa cctccataga cctgaccgta gaagccgttg
    11641 cccagatgct ggaagtagtt ggcttgcagg ttattcacat aggcatcgtt ctgcacatac
    11701 tcgcgcacat gggtacgaac gcgcggtaag tgcgagtcct gtggcggatt ggtgtagtta
    11761 aacttgtcgt agttgttggc aagatttgca aacaggctgc cgatggtcag cagatggtcc
    11821 gtcagccaca aatctgccgt tcccatcacg cccagctgat acatgtaaaa gttttccggg
    11881 ccaccgaccg actggttcag caccggatcg atatggaaat caaagcgtga tttgtcgata
    11941 taccagccct gctcggtgga ctgcggaacc actggctcga cgcgtttttg cgccagcgtc
    12001 gtttcgtggc ccaacggctc tccggcgaga tgatttttca ggctggcgac atcggtttcc
    12061 gtcgtcacct gcggcatgtt aaggcgattt tccgtaatgc ggatcgtttt gatcccatcc
    12121 ggcagatcgt tcatcacgat ccgattagca cggatgatcc cttcgcgcga atcacgatat
    12181 ttcacctgct cgccggtaac atacagcgta tcgcctttcg cctggatctg cggatcggca
    12241 agtccggcat tgtatttcag caacgttaac tgattcgcca ccaccgagtg ctgcaaaatg
    12301 gcatcctgcg gctgcggttg atattgcggg cgggcgttat cgatgtagga cgggcgcaga
    12361 tcgttaaagt tggtgcgcag cgtgacgcca aacataaagg tgttgccacg ttcatagcta
    12421 aggttaacgt cggcccaatc ggtaacgcga taaatcgccc cgacgttaaa cttacttttt
    12481 tgctccagct tcccggcaaa atcctgctga taattattac cttcatactc cagtttcagg
    12541 cgcagcggtt gccagggcgt ctggtattcc acgccgccaa acagtgaggc cgggccgtgg
    12601 aacatctgac tgccgtcgat agatcccgct tgtttgtagc tgttatcgcg atagcaatat
    12661 ttatcactgg ctgaacagag cggatttttc acattaccgc tggtgcccag atatccccag
    12721 cccaggccga gcgtaaaatc gaacggcccc caggctttgc tggcaacaag atattccgca
    12781 tcaaacagcc ccgtaccgcc gatatcccgc gcaccaaccg ccacttgcgg cagccagtaa
    12841 ctctcttccc acaaacgcag tttgagatcg aaggctttat ctttatacgt ttgatcgcca
    12901 gagaacgctt cgacgctgct gtactgccgg gtgcgcacgt cggtgtaacg cagcgttgtt
    12961 tccagccacg ggaagagttg cactgaagct gagtaataac ggtactgatc gttatcgcga
    13021 tagttcagac tcaactcccc ttcccgtgcc atacgcgcgg tgggagtttg taataatcct
    13081 acgccaccga aatccgactg cgacggacca atgggcgcag gatatgtttc gccgtagcaa
    13141 gctgtgctaa tgcccagcgc cagtaagcta agcagatgtc tttttttcat tattggggta
    13201 tccgctgcgt cagagtctga agaatgtcgg cgttaagggc atcaggcgtc tcactccaga
    13261 cggagtctgc gaggccaaca taaataatgc tgccgggcat cggctctacg tgacgcttgt
    13321 tccagtaagc caccggcgct ttttgcgtgc gtccgtccgg gtaaacgacc cacgcgtagc
    13381 tgcgatccgc accgctgagc aggctctggt cagagagata gctcgccacg tcgcgacctg
    13441 gagtgaatgg ctgattgcca ggatggctga taagcccgaa caacgtgacc gtggacggcg
    13501 gtggtccgac ccacagcgtg tagttgccct gcaacggcgg gttaccgcgt tcggcaacgc
    13561 ggacgatatc gggatcaaga ttgatttttt gcctgcctgt cacttccaat gcctggattt
    13621 gctggcgtaa ggcgttaatg gcggcggcat cgtcggtgct ggaatctgcg ccctgttctg
    13681 ccagccgtgt cagcaacgcc tgctgctgac gcaatgctgc cgccgttgcc agctcttcgc
    13741 taatcaccgc accaggccac cagctgtttg ccagtcgagg ttgccctacc agatcgatta
    13801 aatgctctgc gcccgttagc gttttggcct cactgctgcc attgctgaac accttgacgg
    13861 ttcctgccgc gaagaccgat gacgctccca cactcaaaag caacgcgaca atagtttgtt
    13921 taatcattgt tttgccgcct tgatgagagt ggttttcacc gggaaataat ccgcgccgag
    13981 atactgttcc gactggcgaa tttgcccttc gctgtcgatc cagtagcggt tatgccagcg
    14041 cgcctggtcg gtggagactt cttcgtccag cacgcgaacc agggtttcat cgctgccgac
    14101 cttgacggta tcggtgccat cccatttgaa gactgagcgt gcggtggcgt agcgtacctg
    14161 ctggtactcg gtccagccca tcgtgcgcgt ccatgttgca ccatcaacaa tttgtgcggg
    14221 tttaatcagc gggtcggcgg caaggttatt cacttcaatc aggttgtcgc cgccaagcaa
    14281 agtcttcacc agacgaccat gttgtgtgac aagagtggct tgatcctgag tgacccattt
    14341 ttgttgtccg tcttcagcga aggccagcac cacaaacaat tgcggcccgc cattaagctg
    14401 catgtactgg ctggcgtagg gcatattttg aatatcatcg tccgtcagct gtacgcctgg
    14461 cgtgccgaac agactgtccc acagtgaatt gcccagctct ttagtcgtgg ctgaacaggc
    14521 ctgtaataac aggcagataa gaatgagtgc aggtcgcttc acgactcttc tccagagggg
    14581 cagaataacc acaccggagt gtggttatgg ttaatccctt catatttcaa gccacagatg
    14641 caatgactcg aattattttg gggatacata ctttattact gggtactggt ggttgtggtt
    14701 gttgtggtcc cggtattaga accatcaccg ccaccggttg ccgcgagcgc gacacccacc
    14761 gccgagctta cggtgctgac gctggtcgcg gaggaactcc ccgccgaaac cgacgttgct
    14821 gccgcccctg ccgcttcgcc cacctgtaca ggtgcagccc acacagaagt cgccgcaagc
    14881 gcagatatgg caaaaatgcc atacagaact tttttcataa caatttccct tcattgaatg
    14941 aatggagatt tacccaaaaa catttcgggc gggatcgagt atacacaact aaagcatgcg
    15001 aattacggcg cggggaatta gcgatggtgt tttaggataa ttggaatgaa tatcaaatat
    15061 aagtttcaac ttatatttaa ataacaataa aaatcataaa cttaaaataa aaacaaaagt
    15121 aattaatctg cgtattctcc gaaataaaca acgattaaaa atctatttta ggattaacct
    15181 cacggtatga tttccgttaa atcacagaca aggggattta tctgataaaa caatgccgac
    15241 ttattgtcgg cattgttttt aaggttattt aactcctcat actatgcatt aatgtcgata
    15301 ttggcatcag gctccggttg ccggatgcgg cgtaaacgcc ttatccggcc tacatatcgg
    15361 catcaggcac gcatgatgat taaccgcgcc acgctttata gcggttaatc agaccattgg
    15421 tcgagctatc gtggctgctg atttctttgt catctttcag ctctggcaga atacggttcg
    15481 ccagttgttt acccaactcc acgccccact ggtcgaaggt aaagatgttc aggatcacgc
    15541 cctgagtaaa gattttgtgc tcatacagcg caatcaacgc acccaggctg aacggagtga
    15601 tttcgcgcag caggatggag ttggtcgggc ggttaccttc gaacactttg aacggcacca
    15661 cgtagtcaag cgttgccgga tctttaccct gatcgcgata ttcctgctca accacttcgc
    15721 gggatttacc aaacgccagc gcttcggtct gggcgaagaa gttagacagc agtttctggt
    15781 ggtgatcaga gagcgggtta tgggtgatag ccggggcgat gaaatcgcac ggtaccattt
    15841 tggttccctg gtggatcagc tggtagaacg cgtgctgacc gttagtgcct ggttcacccc
    15901 agataatcgg gccagtctgg taatccacaa cgttaccgtt acggtcaaca tacttaccgt
    15961 tggactccat attgccctgc tggaagtacg ccgcgaaacg gtgcatatac tggtcatacg
    16021 gcagaatcgc ttcagtttcc gcaccaaaga aattgttgta ccagatgcca atcagcgcca
    16081 gcaggacagg caggtttttc tcggcaggcg tggtggagaa atgcttgtcc atcgcgtgtg
    16141 cgccagaaag cagttcaacg aagttatcaa agccgatgga gagaacaatc gacaggccaa
    16201 tcgctgacca caaagagtaa cggccgccga cccagtccca gaactcgaac atgttggcag
    16261 tatcaatacc aaactcgcca acggctttgg cattggtgga aagcgccgca aagtgttttg
    16321 caacgtgttt ttcatcacct gccgctttca ggaaccagtc acgcgcgcta tgggcgttgg
    16381 tcatagtttc ctgagtggtg aaggttttag atgctaccag gaacagcgtg gtttccgggt
    16441 ttactttttt cagcacttca gcgatgtgag tcccatcgac gttagaaaca aagtgcatgt
    16501 tcaggtgatt tttgtacgga cgcagtgctt cggtcaccat gtacgggccg aggtcagaac
    16561 caccgatccc gatgttcact acgtcagtga ttgctttgcc ggtataaccc ttccactcac
    16621 cggagataat cgcttccgag aaggttttca tcttctccag caccgcgttg acttccggca
    16681 ttacgtcttt gccatcaacc agaatcgggg tattgctacg gttacgcagc gctacgtgca
    16741 gcacggcgcg gttttcagtg cggttgatct tctcgccaga gaacatcgac ttaatcgcgc
    16801 ccgccagatc gcactctttc gccagatcct gtaatttcgc cagcgtctct tcagtgatgc
    16861 ggtttttgga gtaatccacc agcatctgat cgtcgaatgt tgcggagaac ttagaaaaac
    16921 gatcgccgtc tttagcaaaa agatcggcga tcgtaacgtc tttcatttca tcgaagtgtt
    16981 tctgtagtgc ctgccaggca gcggtctgcg ttggattgat gtttttcatt agcaatactc
    17041 ttctgatttt gagaattgtg actttggaag attgtagcgc cagtcacaga aaaatgtgat
    17101 ggttttagtg ccgttagcgt aatgttgagt gtaaaccctt agcgcagtga agcatttatt
    17161 agctgaacta ctgaccgcca ggagtggatg aaaaatccgc atgaccccgt cgttgacaac
    17221 cgccccgctc accctttatt tataaatgta ctacctgcgc tagcgcaggc cagaagaggc
    17281 gcgttgccca agtaacggtg ttggaggagc cagtcctgtg ataacacctg agggggtgca
    17341 tcgccgaggt gattgaacgg ctggccacgt tcatcatcgg ctacaggggc tgaatcccct
    17401 gggttgtcac cagaagcgtt cgcagtcggg cgtttcgcaa gtggtggagc acttctgggt
    17461 gaaaatagta gcgaagtatc gctctgcgcc cacccgtctt ccgctcttcc cttgtgccaa
    17521 ggctgaaaat ggatcccctg acacgaggta gttatgtctg aaattgttgt ctccaaattt
    17581 ggcggtacca gcgtagctga ttttgacgcc atgaaccgca gcgctgatat tgtgctttct
    17641 gatgccaacg tgcgtttagt agtcctctcg gcttctgctg gtatcactaa tctgctggtc
    17701 gctttagcgg aagggctgga acctggcgag cgattcgaaa aactcgacgc tattcgcaac
    17761 atccagtttg ccattctgga acgtctgcgt tacccgaacg ttatccgtga agagattgaa
    17821 cgtctgctgg agaacattac tgttctggca gaagcggcgg cgctggcaac gtctccggcg
    17881 ctgacagatg agctggtcag ccacggcgag ctgatgtcga ccctgctgtt tgtcgaaatc
    17941 ctgcgcgaac gcgatgttca ggcacagtgg tttgatgtac gtaaagtgat gcgtaccaac
    18001 gaccgatttg gtcgtgcaga gccagatgta gccgcgctgg cggaactggc cgcgctgcag
    18061 ctgctcccac gcctcaatga aggcttagtg atcacccagg gatttatcgg tagcgaaaat
    18121 aaaggtcgta caacgacgct tggccgtgga ggcagcgatt atacggcagc cttgctggcg
    18181 gaggctttac acgcatctcg tgttgatatc tggaccgacg tcccgggcat ctacaccacc
    18241 gatccacgcg tagtttccgc agcaaaacgc attgatgaaa tcgcgtttgc cgaagcggca
    18301 gagatggcaa cttttggtgc aaaagtactg catccggcaa cgttgctacc cgcagtacgc
    18361 agcgatatcc cggtctttgt cggctccagc aaagacccac gcgcaggtgg tacgctggtg
    18421 tgcaataaaa ctgaaaatcc gccgctgttc cgcgcgctgg cgcttcgtcg caatcagact
    18481 ctgctcactt tgcacagcct gaatatgctg cattctcgcg gtttcctcgc ggaagttttc
    18541 ggcatcctcg cgcggcataa tatttcggta gacttaatca ccacgtcaga agtgagcgtg
    18601 gcattaaccc ttgataccac cggttcaacc tccactggcg atacgttgct gacgcaatct
    18661 ctgctgatgg agctttccgc actgtgccgg gtggaagtag aagaaggtct ggcgctggtc
    18721 gcgttgattg gcaatgacct gtcaaaagcc tgcggcgttg gcaaagaggt attcggcgta
    18781 ctggaaccgt tcaacattcg catgatttgt tacggcgcat ccagccataa cctgtgcttc
    18841 ctggtgcccg gcgaagatgc cgagcaggtg gtgcaaaaac tgcatagtaa tttgtttgag
    18901 taaatactgt atggcctgga agctatattt cgggccgtat tgattttctt gtcactatgc
    18961 ttatcaataa acgagcctgt actctgttaa ccaacgtctt tatcgagaat aattgccttt
    19021 aattttttta tctgcatctc taattaatta tcgaaagaga taaatagtta agagaaggca
    19081 aaatgaatat tatcagttct gctcgcaaac gaattcccgt acactgaact ctctggctca
    19141 ctgagggcac catcatggca ctcccccgta ttacccaaaa agagatgact gaacgcgagc
    19201 agcgtgaact taaaacactg ctggatcggg cgcggattgc gcatggtcgc gtactgacca
    19261 actccgaaac caacagcatt aagaaagagt acatcgataa attaatggtc gagcgtgagg
    19321 ctgaagcgaa aaaagcccgc caattgaaga aaaagcaggc ttataaaccg gatcctgaag
    19381 cgtcatttag ctggtcagca aatacgtcga cgcgcggcag gcgctaatga acaatcaacg
    19441 cctgagattt tcactcgtca gtgtgaacac aattttcccc tcaccctaac ccttttcccg
    19501 aaggggcgag gggactgtcc gagcactttt gggccgtttt catcagtctg aggcgttaat
    19561 cagcgcccct tctttttacg ccccggcgag gtaaaacgct taccgttgga cgccgggcga
    19621 ccgccttttt ccgccgtttt ttccatctta acgactggac gtttgatgcc cgctgttttc
    19681 ggtttggcct tcgctttggg cttcgcctcg gaagaggaat tttcaatgag cttaaagaga
    19741 tcgataagct cgtcgtcggt taaatcgcgc cattctccca gcggaatgcc gcttaagctg
    19801 acgttcataa tgcgcgtacg ttccagcttt ttcacttcat agccgaaatg ctcgcacatg
    19861 cgccggatct gacggttcaa cccctgcacc agggtaatgc ggaagacaaa cggtgcttct
    19921 tttttgactt tgcacttttt cgtcaccgtc ccgaggatcg gcactcccgc ccccatacca
    19981 cgaataaatt catcggtaat cggtttatcg accgtcacca gatactcttt ctcatgatcg
    20041 ttacccgcac ggaggatctt attcaccaga tcgccgtgat tggtgaggaa aatcagcccc
    20101 tgggagtctt tatccaggcg gccaatcggg aacacgcgtt tgctgtggtt aacgaaatcg
    20161 acaatgttat cgcgctcgcc atcttcggtg gtgctgacaa taccgacggg cttgttcagg
    20221 gcgataagta ccaaatcttc ggcttcccga ggctcaatca actgaccgtt tactttcaca
    20281 atgtcgccgg gtttcacctg atcaccaatg gtggctcgct tgccattaag gaacacattg
    20341 ccttgctcga tatagcgatc cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat
    20401 ttatttaaac ggactgatga gtcgggcagc ataaattctc ctgtaaaacg cggaatatac
    20461 cgtacctctt agcggggaaa aacagggtta tgcggcaacg tcgaaattaa atgtgatctg
    20521 ccacgccttt tatcacaaaa gtgtctccgg aagtacgatg caaatttgcc tggcaaaata
    20581 aggataatca acatattacg aatcgctgca cacctgtgct gatgactact acgcagagaa
    20641 acaaatgtgc aagagaaagc gctatttctc cccatgcaaa gagtgaaaaa tcgctttgaa
    20701 aggccctcat aatggggtta tatcagatca agcggtgaga aagcgtattt gcacaaatgt
    20761 gcagaaccag gaggcggcaa tggaaaacag tgacgatatc cgtttgattg tgaagattgc
    20821 ccaactctat tacgaacagg atatgacgca ggcgcaaatc gcgcgcgaac tgggtattta
    20881 ccgcaccacc atcagccgct tgcttaaacg aggccgcgat cagggaattg tcaccatcgc
    20941 catcaactat gactacaacg aaaatctctg gctggagcag caactgaagc aaaagtttgg
    21001 cttgaaagac gttgtggtgg tgtcgggaaa tgatgaggat gaagagactc aactggcgat
    21061 gatggggtta cacggcgcgc aactgctgga tcgcttgctg gagcctggcg atattgtcgg
    21121 tttttcctag ggtcgcgcgg tgagcgcact ggttgaaaac ttgccgcaag cggggcaatc
    21181 gcggcagtta atctgcgtgc caattattgg cggaccgtcc ggtaaactcg aaagccgcta
    21241 tcacgtaaac acattaacct acagcgtggc agcgaagctg aaaggggaat cgcatctcgc
    21301 ggattttccg gctctgctgg ataacccctt aattcgtaat gggatcatgc agtctcagca
    21361 ctttaaaacc atctctgcct actgggataa tctggatgtc gccctggtgg gaattggctc
    21421 accggccatt cgcgacggcg ctaactggca tgcgttttat ggtggtgaag agagtgacga
    21481 cctgaaagca ggcgcggtat tccattggca ttgccatgag tgaagaaaaa tacagcggaa
    21541 ttgttggtgc actgcgtgga aaatatatta attgtctggt aacgaatagc agcacagctg
    21601 aactattact gaaataaaaa tcttcccgat gtttaccgta ataaaaatta cggcgggtcc
    21661 ctattatcta aaatcaggag tctgttatgc aaacgtggtt aaatttgcag gataaaatca
    21721 ttatcgtcac cggcggcgca tccggtattg gtctggcgat tgtagaggaa ttattagcac
    21781 aaggcgcaaa cgtacagatg gtagatattc acggtggcga tggtcaatat gaagttcata
    21841 aaggctatca gttttggccg accgatattt ccagcgccaa agaggtaaat catacggtag
    21901 cggaaattat ccagcgtttt ggtcgcatcg acggtctggt caataacgcc ggggtcaatt
    21961 tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca gtatgaactc aacgaagctg
    22021 cattcgaaaa aatggtcaat atcaaccaaa aaggcgtttt tctgatgtcg caggcggtgg
    22081 cgcgccagat ggtcaaacaa catgatggcg tgattgtgaa tgtttcctca gaaagtggac
    22141 tggaaggctc agaaggccaa agctgttacg ccgctaccaa agccgcgctc aatagcttca
    22201 cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg tgtggtcggt atcgcgccgg
    22261 ggattctgga aaaaaccggg ctacgcacgc cggaatatga agaggcgctg gcgtggacgc
    22321 gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa aaacgccatt cctattgggc
    22381 gcgccggaag attagcagaa gtcgctgatt ttgtttgtta tctgctgtct gaacgcgcca
    22441 gctacatcac cggagtaacc actaacattg cgggcggcaa aacgcgcgga taaggaggct
    22501 ttatggtcaa cgccattttt tgtgcccacg gcaaattagc ttgcgccatg ctggaatcgg
    22561 ttcagatggt gtacggcgac gccaatgtcg aggcggttga atttgtacca ggcgagaacg
    22621 ccggagacat cgtagcaaag ctggaaaagt tagtaagcat tcacaatcaa gatgagtggc
    22681 tgattgccgt ggatttacag tgcggcagcc cgtggaatgc ggcagcgatg cttgcgatgc
    22741 gcaatcctcg tctgcgggta atcagcggcc tttccctgcc gctggcgctc gaactggttg
    22801 ataaccagga cagcatgaat gttgatgaac tgtgtgaaca cctgacgcaa atcgcaaagc
    22861 aaacctgcgt tgtctggaaa caactggcaa cggcagagga agatttctga tgaacattac
    22921 gctcgcccgc attgatgacc gtctgatcca cggccaggtc actaccgtct ggtcgaaagt
    22981 ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt tataacgatg aagtgcgccg
    23041 gacattattg cgtcaggctg cgccaccggg aatgaaggtt aacgtggtca atattgaaaa
    23101 agccgtcgcg gtttatcaca acccgcaata ttaggacgaa accgtttttt atcttttcac
    23161 tcgcccacag gatgccttag cgatggttcg ccagggggta aaaataggca ccctgaatat
    23221 tggcggcatg gcctggcggc ccggtaaaaa acagttaacc aaagcggttt cattagatga
    23281 tgatgatatt aacgcatttc atgagttaaa taatcttggc gtcataatgg atttacgcgt
    23341 cgtagcgtca gatccctcaa tcaatattat cgacaaaata aacgaacagt taatcgcaaa
    23401 ttaaataata gcgcctgtgt acatatgctt taacaggcag ggatgacgac acttaaaagg
    23461 tgacaaatca tggaaataag taccctacaa attattgcta tatttctttt ttcctgtatt
    23521 gccggaatgg gcagcgtgct ggatgaattt cagactcatc gcccgttaat tgcctgtacg
    23581 gtgattggtt taattctcgg tgatttaaaa accggaataa tactcggtgg tacgctggaa
    23641 ttgatagctc tcggctggat gaacgtcggc gcggcgcaat ctccggattc tgcactcgcc
    23701 agcataatct ccgccattct ggttatcgtt ggtcagcaaa gcatcgccac cggaatcgcc
    23761 atcgcgttgc ctgtggctgc ggcaggccaa gtgctgacgg tgtttgcccg taccatcacc
    23821 gtagtgttcc agcacgcggc ggataaagca gcggaagagg cgcggtttcg cactctcgat
    23881 attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg ttgctattcc ggcactgatt
    23941 gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt taaacgccat tcccgaattt
    24001 gtgacccgtg gactgcaaat tgctggcggt tttatcgtgg tggtcggtta cgccatggtg
    24061 ctacgtatga tgggcgtgaa atatttgatg cctttctttt tcctcggttt cctcgcaggt
    24121 ggctacctcg atctcagcct gctggcgttc ggtggcgtcg gcgtgatcat ggccctggtc
    24181 tacatccagc taaatccaca gtggcgtaaa gctgaacctc aactccaggc caccgcctcc
    24241 accgcccttg accaacttga cgattaacgg agccatcatg gaacagagaa aaattacacg
    24301 cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa caggcgtcct ttaactttga
    24361 acgtattcac ggactgggct tttgctacga catgatcccc gccatcaagc gactttaccc
    24421 attaaaagag gatcaggttg cggcgctcag gcgacacctg gtgttcttca ataccacgcc
    24481 agccgtatgt ggcccggtca tcggcgtcac tgccgccatg gaagaggcgc gggccaacgg
    24541 cgcggaaatt gatgacggta ccattaacgg catcaaagtc ggtctgatgg gaccattggc
    24601 aggagttggc gatccactgg tctggggaac gctgcgcccg attaccgctg cgctcggcgc
    24661 atctctggca ctttctggca acatcctcgg cccgctgctg tttttcttta ttttcaatgc
    24721 ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc tttcgcaaag gggtgaatat
    24781 cgtcagcgat atgggcggga atgtgctgca aaaactcacc gaaggcgcgt cgattctcgg
    24841 actgtttgtg atgggcgtgc tggtcaccaa atggacgtca atcaacgtac cgttggtggt
    24901 ttcacaaacg catgccgccg atggctccac cgtcatcatg accgtgcaga acattctcga
    24961 ccaactttgc cctggtttgc tggcgctcgg tctgacgcta ctgatggttc gtctgctcaa
    25021 caaaaaaatt aacccggtat ggctgatttt cgccctgttt ggcttaggga ttatcggcaa
    25081 tgcgctgggc ttcctgtcct gattcttcgc cccggcacga ctgccggggc catcgctcaa
    25141 catgaggtgg tttatgaaaa caacagctct gcgtctttat ggtaaacgtg atttacgcct
    25201 ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc ctcgcgacgg tggtcactga
    25261 cagcctgtgc ctctcttcct ggaaagaggc aacctgggtg aaaaccataa aaaagtaccc
    25321 gacgatgtgg cgaccaaccc aatcatcatc ggccacgagt tttgcggcga tattctggcc
    25381 gtgggtaaaa agtggcagca caaattccag ccgggtcagc gttatgtgat tcaggccaac
    25441 ctgaaactcc ctgaccgccc ggactgcccc ggctactcct tcccgtgggt gggcggcgaa
    25501 gccacgcatg tggttattcc caacgaggtc atggaacaag attgcctgct ggcatacgac
    25561 ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt cctgcgtgat tggcgcgttc
    25621 aacgccaact atcatcttca ggaaggtagt tataaccaca cgatggggat tcgcccgcaa
    25681 gggcgcacgc tgatcctcgg cggcaccgga ccaatgggat tgttggcgat tgattatgcg
    25741 ctgcatggac cagttaaccc gtcgctgctc gtcattaccg ataccgacaa cgacaaattg
    25801 agttatgcgc gcaagcacta tccatcagaa ccgcaaacac tgattcatta tctcaatgcc
    25861 accgatgcag catttgatac gttaatggcg ctgagtggcg gtcacggctt cgatgatatt
    25921 ttcgtctttg tgcctaatga aggactggtg actctcgcct cttccttgct ggcgacagat
    25981 ggttgcctga atttcttcgc cggaccgcag gataaacatt tcagcgcgcc aattaatttc
    26041 tacgatgtgc attatgcatt tacccactac gtgggcacgt caggcggcaa taccgacgac
    26101 atgcgcgcgg cggtcaaact gattgaagag aaaaaagtgc aggccgcaaa agtggtaaca
    26161 catattcttg ggctgaatgc cgcgggcgaa accacgcttg aattgcctgc cgtcggcggc
    26221 ggcaaaaagc tggtgtatac cgggaaatac ctgccgctga cgtcactcac gcagattcag
    26281 gatcaagcac tggcggcgat tctggcgcgt catcagggga tctggtccgg tgaggcggag
    26341 caatatctgc tcactcatgc agaggcaatt tcccatgatt aatcgcgcta cttaactctg
    26401 catgtcgctg gctggtcgcc gcggcaactt cgcgacctga tgtgcggctg attgctgaag
    26461 cggcccaatg tgcacgagaa ggataaaaaa acatcagcgc cttaaggggc gctcaaattg
    26521 gtggcaaagt acacattgtt tatgccggat gcggaataaa gccttattcg gcctacataa
    26581 tctcgaaaat tcaatatatt gcagttatct ggtaggcctg gtaagcgtag cgcatcaggc
    26641 aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata cgtgacatcg tgagccatgt
    26701 cagtaagtgg ttaaattcgc agtggtagca tgccttccgt gatttcttac taaccggaga
    26761 agtaatggaa ctgcttttat tgagtaactc gacgctgccg ggtaaagcct ggctggaaca
    26821 tgcactgccg ctaattgctg aacagttgca gggtcgccgc tcagcggtgt ttatcccttt
    26881 cgctggcgta acgcagacct gggatgatta cacagcgaaa acggctgcgg ttctcgctcc
    26941 gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat cccgttgccg cgattgaaaa
    27001 tgctgagatc gtgattgtcg ggggcgggaa tactttccag ttgctgaaac agtgccgcga
    27061 gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt ggtgcgctgt atattggctg
    27121 gagcgcaggc gctaaccttg cttgcccaac cattcgtacc accaacgata tgccgattgt
    27181 cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg caaatcaacc cgcacttcac
    27241 caacgcgctg ccggaaggcc ataaaggtga aacccgtgag cagcgtattc gcgaactgct
    27301 ggtcgtcgcg ccagaactga cgattattgg tctaccggaa ggtaactgga tcacggtgag
    27361 taaaggtcac gctacgctgg gtggcccgaa caccacttat gtgtttaagg ccggtgaaga
    27421 agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat tgccggatgc atgatccggc
    27481 atcttgttca tagtgcctga tgcgacgctt gacgcgtctt atcaggccta caggattcac
    27541 aatattaata ctcgtcccgt ccttcatctt catccggctg ttccagcaca ctgtaagcca
    27601 ccgaacaaaa cagcgagttc agacgctgca tatctcccag taagcctaaa tgtagcgaac
    27661 tggtttcaat gctctgcacg ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc
    27721 ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa acgacgagcg ctggtgacat
    27781 cgccagagaa gaacaccgac attgccagct ttaaattact gagcaattgc tcatagagcg
    27841 catccagttc cttcaaccca tcaagcgaaa atgcccgccg tgctgccagc gatttatcag
    27901 caatttcgct gcccatgcgc tcgacgatat cggaagcctg ttcaaggttg agcgacattt
    27961 caataatctc cgcccagcga cgcgactctt cttctgccag ttcctctttt ggcattcgcg
    28021 ccagatacag cttaatggcg gtgtagagaa cgttgatatc atctgccagc ttacgcagct
    28081 ctttctcctg ccgtggctcg ccgtgcatca ctttattcag cccttccatc atctgttcca
    28141 tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtat
    28201 ccagcgcgct gacatccaga tgtttaggcc gtagctgggt atccagttcc ggttcatcgc
    28261 gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa tggcagcatg accaggcaac
    28321 gtacaaggtt gtagaagacg tggaaataga tcaccagttc cgcttttggc aatgacaact
    28381 tccccattgt ctctgccagc aaatggacaa acggcaggat aatcaggcta cccaccagct
    28441 taaacagcaa actacccagc gcgacacggc gggctgcggc attggcggca ctgttgttga
    28501 gcatcgccag caggccggaa ccgaggttag caccaatcac cagacagagc gccacgggga
    28561 aggagataat gcctgcggcg gtcagagtcg cggtcagcag tacagcagca aggctggagt
    28621 agctgataat ggcgaacatc gcgccaatca gcgcatccag cagaatatcg ccggtcagcg
    28681 aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca
    28741 actccagcgc cagcaaaatc agcccaagac caataccgac gcggcccagt tgcccggcgc
    28801 gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa aataagtaac ggtgacagcc
    28861 aggataagtc gaaggtgaga atacgcgcca ttagcgccgt cccgacatct gcacccagca
    28921 caatcaccag agccggtgcg agggctacca gatcctgagc gacgaacgag gtcaccagca
    28981 tggtggtggc attactgctc tgtaccagtg cggtaacgcc gatccccgcg caaaaggcga
    29041 gcggcttttt ttcgacgctc cggctaagga cagtacgcaa acgcgcgccg aagacgcgca
    29101 ttacgccggt tcgaacaata tgagtccccc agaccagcag ggcgacggca gaaagcaggt
    29161 gaagcagcgt taacacgagt tcaggttctc cttatcgtta tatgttcctg aagcaattga
    29221 atgaccaaag gaccggaaac acgctgactg gcatctttac tgtgcctttt gacattcgtt
    29281 tttccggcta agattattcc agcctgtttc cagtataagt gtttcatatt aataaagaga
    29341 cagggcactc attgagcgcc ctgtttgttg taaggaaaag tgacagattt gtgaatcagt
    29401 ccgcgtcata ccccagattc ggtgccagcc agcgctcaac atcggagacg ctcatacctt
    29461 tacggcgggc atagtcttca acctgatcgc gctgaatttg cgccaccgcg tagtacttgc
    29521 tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc aggccacatg gcgaaagatt
    29581 ctgtgagttt catgccagta tgtttttcca cttccagcag ctcccagatg gtggcttttt
    29641 ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg gatgccctgg tagttttcgc
    29701 ggatcagttc ttcgttgctg aggttctcgt tcggcgcata gccccagtag actttacgca
    29761 cacgctcatg gagatactcc gcaaaggctt cggctaaacg gtcggcaagc gctttcacca
    29821 tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc agccagtgcg tcctcttcca
    29881 gcccgccagt cacggcaaat gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa
    29941 cgaagtcagc gagacagtag ttagcgaatc ccgttttttc ggtctgttga cgcagatggt
    30001 ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca
    30061 cacggtttgc cgggaacaag cccaccacgc cacgcggatt cagcgttttc tcggcgctta
    30121 atttatccag catgtcgttg gcgtctttaa acagccgctg cgcctcaacg cccaccactt
    30181 catcttccag aatgcgcgga tacttcccgg ccagcgacca ggtcataaag aacggtgtcc
    30241 agtcgatgta attacgcaga gtttcgatgc tggcttcaac ttcctgcacg ccgagacggt
    30301 gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa gtcgttatcg cgcgccgctt
    30361 ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg ctgaatacgt acggtttcat
    30421 actccttgcg ggtacgagcg acaaaatcat cgcgctgagt atcggaaagc agcgccgcca
    30481 ccacaccaac ggtgcgcgag gcattctgca catacaccgt cgggccgctg tagttctgct
    30541 caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc accgatcagt aacggaatgg
    30601 tgaagccctg acgctccatc tctttcgcca cgttaaccat ctcgtccagc gacggcgtga
    30661 taagccccga aagtccaatc agatcagcat tcacttcttt agcggtacgg agaatttttt
    30721 ccgcaggcac cataacgccg agatcgacaa tttcgtagtt gttacattgc agcaccacac
    30781 caacgatatt tttaccgatg tcgtggacgt cgcccttcac ggtggcgatc accatcttgc
    30841 cgttggtttt gccctgctct ttgctggctt caataaacgg ttcgaggtag gccactgcct
    30901 gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa cattttcccc tcgccaaaca
    30961 gatcgccgac cacgttcatg ccgtccatca acgggccttc aatcacttca atcgggcgcg
    31021 tagcctgctg gcgggcttct tcggtatcct gctcgataaa ctcagtaatg cctttgacca
    31081 gcgagtattc cagacgttta ttcacttccc acgagcgcca ctccgcctgc tgggcgttag
    31141 cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag ctccagtaaa cgttcagtgc
    31201 catcatcgcg acgattaaga atcacatctt ccaccgcgtc gcgcagttca gcgggcaggt
    31261 cgtcgtaaat cgccagttgc ccggcgttga cgatccccat atccatgcca ttgcgaatag
    31321 cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg ttcgttgcca cggaatgaga
    31381 aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg cagttcgcgt ttgatgtctt
    31441 cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc ttcaatgccg gtggcgaccg
    31501 cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa gccaacctct tcggtgagga
    31561 ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg ggtatcggcc tgtccctgtt
    31621 cgtcaaaggc cattaccacc accgctgcac cgtaacgacg caacagtttc gcatgatgga
    31681 taaaggcgtc gacgccctct ttcattgaga tagagttaac aatgcctttg ccctgaatac
    31741 acttcagccc cttttcgatg acctcccatt ttgaggagtc gatcataatc ggcacgcgag
    31801 cgatatccgg ttcaccggca atcagattga gaaaacgcac catcgccgct tcggcgtcga
    31861 gcatcccttc atccatattg atatcgataa tctgcgcgcc gttttccacc tgctggcggg
    31921 ccacatccag tgcctcgctg tatttctctt ctttgatcag gcgcttgaac ttagcggaac
    31981 cggtgacgtt ggtgcgttca cccacgttca caaacaggct atcttcgcca atgttcagcg
    32041 gctccaggcc ggacaaacgg caggctacgg ggatttccgg cagtttgcgc ggcgctaatc
    32101 cttctactgc acgactcatc gctgcaatat gttgtggcgt ggtgccacag cagccgccga
    32161 cgatattgag aaagcccgct tccgcccatt cacgtatttg ttttgccatc gtgtcggcgt
    32221 cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt cgggtgcgcg gtgacgtagc
    32281 attccgcaat ccgtgacagc tcctgcacgt actggcgcag ttcatcgggc cccagcgcac
    32341 agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga gttgtaaaat gcttcggtgg
    32401 tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt gccagagatc ataatcggca
    32461 gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa taccgccgct ttggcgttaa
    32521 gggtgtcgaa aacggtttca atcaggatca gatccgcacc accttccacc agcgctttgg
    32581 tggactctcg ataagccgcc accagcccgt caaaagtgat attacgaaat gccggatcgt
    32641 tgacgtccgg agaaatagac gccgtgcggt tggttgggcc gagaacaccg gcaacgtagc
    32701 gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca ggcgcgcgcc agtttcgccg
    32761 ccgcaaagtt gatttccgcc gacagggatt ccatctggta atccgccatc gcaatggtcg
    32821 tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga
    32881 tagcggcgat cacttccggt ttactgagta ccagcaggtc gttgttgcct ttgaggtcgc
    32941 atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc gttcagtcga tagctctgga
    33001 tcatggtgcc cataccgccg tccagcacca gaatacgttc atttaactgc gcacgcagtt
    33061 gttccacttt gctgctcaca cttgctcccg acacgctcaa cccagattta ataaaaattc
    33121 aacaaaccat actggcataa acgcatctgt ggtaaaagcg accaccacgc aacatgagat
    33181 ttgttcaaca ttaactcatc ggatcagttc agcaactatt gcattaacta acaataaaaa
    33241 tgaaaatgat ttccacgata cagaaaaagg agactgtcat ggtcgcaccc attcccgcga
    33301 aacgcggcag aaaacccgcc gttgccaccg caccagcgac tggacaggtt cagtctttaa
    33361 cgcgtggcct gaaattactg gagtggattg ccgaatccaa tggcagtgtg gcactcacgg
    33421 aactggcgca acaagccggg ttacccaatt ccacgaccca ccgcctgcta accacgatgc
    33481 aacagcaggg tttcgtgcgt caggttggcg aactgggaca ctgggcaatc ggcgcacatg
    33541 cctttatggt cggcagcagc tttctccaga gccgtaattt gttagcgatt gttcacccta
    33601 tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa
    33661 gcgatcacga agcgattatt atcgatcagg tacagtgtac tcacctgatg cgtatgtccg
    33721 cacctatcgg cggtaaattg ccgatgcacg cttccggtgc aggtaaagcc tttttagccc
    33781 aactgagcga agaacaggtg acgaagctgc tgcaccgcaa agggttacat gcctataccc
    33841 acgcaacgct ggtgtctcct gtgcatttaa aagaagatct cgcccaaacg cgcaaacggg
    33901 gctattcatt tgacgatgag gaacatgcac tggggctacg ttgccttgca gcgtgtattt
    33961 tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc cggaccgatt tcacgtatta
    34021 ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa agcggcgaag gaagtgacgc
    34081 tggcgtacgg tggaatgcgc tgactttttc tggcgggcag aggcaatatt ctgcccatca
    34141 tacctgagtg gcaatagaat aagggtgtct gttaatcgca ttgacgccaa aataacttaa
    34201 tgtcatacac ttcacacact tttattaact cagcattatt tttaaacatc aaaccactta
    34261 attattaaca aaatcgcaag atctcattcc accgaaactt ttacggctgc attatgagat
    34321 tgagcgattt atgatatttc tttagttgta atcatattaa attttttcac tgttctgata
    34381 gttaaaattt aagacatcaa taaacaatga gatatttaaa tgattactcg tattcctcgt
    34441 agttctttct ctgcaaatat taataataca gcccagacaa atgaacacca aaccctgagt
    34501 gaattgtttt ataaagaact cgaggataaa ttttctggca aggagctggc gacgcctcta
    34561 ttaaaaagct tctcagagaa ctgtagacaa aatggtcgtc atatctttag caacaaggat
    34621 tttgtcatta aattttccac gtccgtctta caagctgata agaaagaaat tacgataatt
    34681 aataaaacga aaacacgaca ctcactcaaa ccattgctcc aatatttgaa gaatacctaa
    34741 tggaaatttt acctcaacgc tcagacactc ttgataaaca agaattaaac ctaaaatcag
    34801 atagaaaaga aaaagaattc ccaagaatta aacttaatgg tcaatgttat tttccggggc
    34861 gaccccaaaa ccgtatagta tgccgacaca ttgctgcaca atatattaat gatatttatc
    34921 agaatgttga ttacaaaccc catcaagatg attattcttc agctgaaaaa tttctcactc
    34981 acttcaacaa aaaatgcaaa aaccagactt tggcgttgat ttccagccgt cctgaggggc
    35041 gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa agcatatttt gacaagatgg
    35101 aatcaaatgg catcagtgtt atggcagcca tattactggt ggataaccat gctttgacgg
    35161 tccggctaag aataaagaac acaactgaag gatgtaccca ttacgtggtt tcggtttatg
    35221 atcctaatgt aactaacgac aaaataagaa tcatgagcga aagcaaagag gatattaaac
    35281 actattctct gatggatttt atgaatgtag attatagcct cctgaaatgg tcaaatgatc
    35341 atgttattaa ccaatctgtt gcaataattc cagcacttcc gaaagaacag ctattgatat
    35401 taaaaggatc
//
LOCUS       DAZRVY010000031        34981 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZRVY010000031 DAZRVY010000000
VERSION     DAZRVY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.031"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHXOX_001560"
     CDS             complement(<1..815)
                     /locus_tag="ACHXOX_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7005058.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHXOX_001561"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHXOX_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHXOX_001562"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHXOX_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHXOX_001563"
     CDS             complement(1397..1873)
                     /locus_tag="ACHXOX_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR7005059.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHXOX_001564"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHXOX_001564"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR7005060.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHXOX_001565"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHXOX_001565"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR7005061.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHXOX_001566"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHXOX_001566"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR7005062.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHXOX_001567"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHXOX_001567"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR7005063.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHXOX_001568"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHXOX_001568"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR7005064.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHXOX_001569"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHXOX_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR7005065.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHXOX_001570"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHXOX_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR7005066.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHXOX_001571"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHXOX_001571"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR7005067.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHXOX_001572"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHXOX_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR7005068.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHXOX_001573"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHXOX_001573"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR7005069.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHXOX_001574"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHXOX_001574"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR7005070.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHXOX_001575"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHXOX_001575"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR7005071.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHXOX_001576"
     CDS             13839..14543
                     /locus_tag="ACHXOX_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR7005072.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14814..15461
                     /locus_tag="ACHXOX_001577"
     CDS             14814..15461
                     /locus_tag="ACHXOX_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR7005073.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(15526..16237)
                     /locus_tag="ACHXOX_001578"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="ACHXOX_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHXOX_001579"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHXOX_001579"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR7005074.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="ACHXOX_001580"
     CDS             complement(17475..18236)
                     /locus_tag="ACHXOX_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR7005075.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="ACHXOX_001581"
     CDS             18369..18779
                     /locus_tag="ACHXOX_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR7005076.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="ACHXOX_001582"
     CDS             complement(18741..19847)
                     /locus_tag="ACHXOX_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7005077.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="ACHXOX_001583"
     CDS             complement(19858..21000)
                     /locus_tag="ACHXOX_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7005078.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHXOX_001584"
     CDS             complement(20993..22729)
                     /locus_tag="ACHXOX_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7005079.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHXOX_001585"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHXOX_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR7005080.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHXOX_001586"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHXOX_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR7005081.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHXOX_001587"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHXOX_001587"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR7005082.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHXOX_001588"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHXOX_001588"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR7005083.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHXOX_001589"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHXOX_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR7005084.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHXOX_001590"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHXOX_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR7005085.1"
                     /translation="MDYRKIIKEIGRGKKHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHXOX_001591"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHXOX_001591"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR7005086.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHXOX_001592"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHXOX_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR7005087.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHXOX_001593"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHXOX_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR7005088.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHXOX_001594"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHXOX_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR7005089.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHXOX_001595"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHXOX_001595"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR7005090.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHXOX_001596"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHXOX_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8271 a         8975 c         9490 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaaa
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg cattttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZRVY010000032        34771 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZRVY010000032 DAZRVY010000000
VERSION     DAZRVY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.032"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHXOX_001597"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHXOX_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHXOX_001598"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHXOX_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR7005091.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4881..5543
                     /gene="acrS"
                     /locus_tag="ACHXOX_001599"
     CDS             4881..5543
                     /gene="acrS"
                     /locus_tag="ACHXOX_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR7005092.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(5546..5725)
                     /locus_tag="ACHXOX_001600"
     CDS             complement(5546..5725)
                     /locus_tag="ACHXOX_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR7005093.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="ACHXOX_001601"
     CDS             complement(5809..6693)
                     /gene="yhdJ"
                     /locus_tag="ACHXOX_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR7005094.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6779..7075)
                     /gene="fis"
                     /locus_tag="ACHXOX_001602"
     CDS             complement(6779..7075)
                     /gene="fis"
                     /locus_tag="ACHXOX_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR7005095.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="ACHXOX_001603"
     CDS             complement(7101..8066)
                     /gene="dusB"
                     /locus_tag="ACHXOX_001603"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR7005096.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="ACHXOX_001604"
     CDS             complement(8395..9276)
                     /gene="prmA"
                     /locus_tag="ACHXOX_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR7005097.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(9288..10739)
                     /gene="panF"
                     /locus_tag="ACHXOX_001605"
     CDS             complement(9288..10739)
                     /gene="panF"
                     /locus_tag="ACHXOX_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR7005098.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10729..10971)
                     /locus_tag="ACHXOX_001606"
     CDS             complement(10729..10971)
                     /locus_tag="ACHXOX_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR7005099.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(11080..12429)
                     /gene="accC"
                     /locus_tag="ACHXOX_001607"
     CDS             complement(11080..12429)
                     /gene="accC"
                     /locus_tag="ACHXOX_001607"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR7005100.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12440..12910)
                     /gene="accB"
                     /locus_tag="ACHXOX_001608"
     CDS             complement(12440..12910)
                     /gene="accB"
                     /locus_tag="ACHXOX_001608"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR7005101.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13888..14862)
                     /locus_tag="ACHXOX_001609"
     CDS             complement(13888..14862)
                     /locus_tag="ACHXOX_001609"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR7005102.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15014..16954
                     /gene="csrD"
                     /locus_tag="ACHXOX_001610"
     CDS             15014..16954
                     /gene="csrD"
                     /locus_tag="ACHXOX_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR7005103.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17259..18302
                     /gene="mreB"
                     /locus_tag="ACHXOX_001611"
     CDS             17259..18302
                     /gene="mreB"
                     /locus_tag="ACHXOX_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR7005104.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18368..19471
                     /gene="mreC"
                     /locus_tag="ACHXOX_001612"
     CDS             18368..19471
                     /gene="mreC"
                     /locus_tag="ACHXOX_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR7005105.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19471..19959
                     /gene="mreD"
                     /locus_tag="ACHXOX_001613"
     CDS             19471..19959
                     /gene="mreD"
                     /locus_tag="ACHXOX_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR7005106.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19968..20561
                     /gene="yhdE"
                     /locus_tag="ACHXOX_001614"
     CDS             19968..20561
                     /gene="yhdE"
                     /locus_tag="ACHXOX_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR7005107.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20551..22020
                     /gene="rng"
                     /locus_tag="ACHXOX_001615"
     CDS             20551..22020
                     /gene="rng"
                     /locus_tag="ACHXOX_001615"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR7005108.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            22088..25888
                     /gene="yhdP"
                     /locus_tag="ACHXOX_001616"
     CDS             22088..25888
                     /gene="yhdP"
                     /locus_tag="ACHXOX_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR7005109.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            26044..27489
                     /gene="tldD"
                     /locus_tag="ACHXOX_001617"
     CDS             26044..27489
                     /gene="tldD"
                     /locus_tag="ACHXOX_001617"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR7005110.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="ACHXOX_001618"
     CDS             complement(27617..28546)
                     /gene="aaeR"
                     /locus_tag="ACHXOX_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR7005111.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28729..28932
                     /gene="aaeX"
                     /locus_tag="ACHXOX_001619"
     CDS             28729..28932
                     /gene="aaeX"
                     /locus_tag="ACHXOX_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR7005112.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28940..29872
                     /gene="aaeA"
                     /locus_tag="ACHXOX_001620"
                     /pseudo
     CDS             28940..29872
                     /gene="aaeA"
                     /locus_tag="ACHXOX_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            29878..31845
                     /gene="aaeB"
                     /locus_tag="ACHXOX_001621"
     CDS             29878..31845
                     /gene="aaeB"
                     /locus_tag="ACHXOX_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR7005113.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31937..32209
                     /locus_tag="ACHXOX_001622"
     CDS             31937..32209
                     /locus_tag="ACHXOX_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR7005114.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="ACHXOX_001623"
     CDS             complement(32265..32528)
                     /gene="yhcN"
                     /locus_tag="ACHXOX_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR7005115.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32893..33363)
                     /gene="argR"
                     /locus_tag="ACHXOX_001624"
     CDS             complement(32893..33363)
                     /gene="argR"
                     /locus_tag="ACHXOX_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR7005116.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33798..34736
                     /gene="mdh"
                     /locus_tag="ACHXOX_001625"
     CDS             33798..34736
                     /gene="mdh"
                     /locus_tag="ACHXOX_001625"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR7005117.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8495 a         8637 c         9073 g         8566 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa
     4681 agaggcagaa ttaaaacctc gtaaattgaa atatatattc atgtggcgaa tgtattttag
     4741 gtaaataata tatattattt atttgacgat ttatagcaac tcaatgttac cctctcgtat
     4801 aaatacacat tagatgatag attaaccttc gctattttct caccctgtgt cgaatatatt
     4861 tatttcctga ataattaatc atggcaaaaa gaaccaaagc cgaagctctg aagacccggc
     4921 aagaactgat tgaaactgcc atcgcccagt ttgcgcaaca tggcgtaagc aagacgacgt
     4981 tcaacgacat tgccgatgcc gctaacgtta cgcgtggcgc tatctactgg cacttcgaga
     5041 acaagaccca actgtttaat gagatgtggt tgcaacagcc ttcattgcgg gagttaatcc
     5101 aggaacactt gacggctgga ttagagcatg acccgtttca acaattgcgt gaaaaattga
     5161 ttgtcggctt gcaatatatt gccaaaattc cccgccagca ggcgttgctg aaaatcttat
     5221 atcacaaatg tgaatttaat gatgggatgc tggccgaggg agtgatacgc gaaaagatgg
     5281 gctttaatcc gcagactctc cgcgaagtat tgcaggcgtg tcagcaacaa ggttgtgtag
     5341 caaataacct cgatttagat gttgtgatga ttattattga tggtgccttc agcggaattg
     5401 ttcaaaactg gttaatgaat atggcgggtt atgatcttta taaacaagca cccgcgctgg
     5461 tcgataacgt attaagaatg ttcatgccag atgaaaacat aacgaaatta attcatcaaa
     5521 cgaatgaatt aagtgtcatg taaattcaca ttctgtcctg aaaattcagt tcgtcacagt
     5581 taaccagttt tagtggataa actgagttca tattacggca tttgtctgtt tctgttgtca
     5641 gcagttcaat ccactgcatc agtaaagtat caaacagaaa aacgaagaca gcgaaaacga
     5701 cgagccacca atacttgcga atcattacct gagcctgtgc atgtttaacc aagtctggca
     5761 ggaaatatac acgaaatgaa taatctgaaa accaggctta cacttccttt actttgcaat
     5821 gagatcgggg tcaacttcgc ttaaccggct tcgttttgac aggttgccgg tctttctttt
     5881 tttcactttc gccagttctt ccgcggagta atgcgacgcg acatccagcc gtcgaagccc
     5941 cattttgatg tactcgctgt tgatctcaat accaatgaat tttcgtccgc tggcgatggc
     6001 tacggcaccg gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc ctgggttgga
     6061 agaggcgaga ataatgcgtt tcagtaaggc ttccggtttt tgcgtcgggt ggttctcata
     6121 ttcatccatc aaataacgca cgcgcggaaa atcccagacg ttacccggta ctttttgatg
     6181 attgtatggc tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg atccggtttt
     6241 ggcttctacc agaatagcat caccgttgaa tgtgtagttc tttgcgtctt tcaccatcat
     6301 caggatgggt tcgtacatgg agccgtagtg ttttttcgcc tgtactccag aactgtcata
     6361 tgaccagacg atgcgacttt tgatggtaaa aagcttgcgg cactggagat cgataaaggg
     6421 catgttttcc gtactgttca taatgtacat gctgccctgc tttttcagaa cgcggtggca
     6481 ctctacaatc acttcaaaca gccagtcgat aaacagatct tctttccagg cttcgatcag
     6541 accatcaaaa tttttaccga tgttatacgg tgggtcggca aagatcagat cgacgctttc
     6601 agtaggtagc tttttaagtt cggcaagggc atcaccgtga ataatggtct tagcttcatt
     6661 accaaaccgg gtcggttcac atcctgttct catggtcact ccctttgtga cacctataaa
     6721 aaaggcgctt ccccatgccg agtagcgcct ttttaatcaa gcatttagct aacctgaatt
     6781 agttcatgcc gtattttttc aattttttac gcagcgtacc acggttgatg cccatcatca
     6841 gcgcagcacg ggtctggtta ccacgggtgt attgcatcac catgtccaac aggggctgtt
     6901 ctacttcagc cagtaccagc tcatagaggt cattcacatc ctgaccattc agttgagcaa
     6961 aatagttctt cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt acctgatcct
     7021 gagagttaac ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg aacatagttc
     7081 tgtcagctct ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa cgcctccagc
     7141 tgttcgctgg catcctcaat ggcgttgaat gtgcgccgaa actggtcatt tggagcgtgt
     7201 tcctggagat accaggaaac gtgtttacgt gcaattcggt acccttttgc cggaccataa
     7261 aagtcatgca gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc caaaggcagc
     7321 gggggcagca actccccagt gtccagataa tgctggattt cccgaaagat ccagggtctt
     7381 ccctgagctg cgcggcctat catcagggca tccgcccctg tatagtcgag cacagctctg
     7441 gctttaagcg ggtcagtaat gtcgccattc gcgataaccg gaatggaaac tttctgctta
     7501 actgcccgaa tactgtcgta ctcagcttct ccattgaaca aacaggcgcg tgtacggcca
     7561 tgaatggtca gagcctgaat gccacagtct tcagccagtt gggcaatctc ttcgcagtta
     7621 cggtgttccg gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc cactgcattg
     7681 acgacctcgg taaggatcga tttaacgaca tccgggtact gcaagagggc tgaacctgcg
     7741 agcttgcgat tcactttttt agccgggcaa cccatattga tatcaataat ctgggcaccg
     7801 ctttccacgt taatacgtgc tgcatctgcc atttctttcg gatcgctacc agcaatttgc
     7861 acggtgcgaa taccgggttc atcaatgtgc accatccgta aacgagattt gtcgctttcc
     7921 caaacctgtg ggttagaaga catcatctcg gatactgtca atccggctcc catctcgtag
     7981 cacaacgtcc gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat caggcgattt
     8041 ctgagctgat attgtccgat gcgcatgagt taagaaatga ccatactgtg actgcaaggc
     8101 ggcgtatatt acgcattttt tgcacgagat gaaaggccaa actttgacca atcctctgag
     8161 atggatcaaa gaattgcatt taaaatgagc gtggtgagat aattactcat aaaaatcatc
     8221 atattagaaa atagtgacta aaatttacac tcaaagaaat ttgagtaagt tctcaatttt
     8281 tctttatgaa tgaaaatttt ggcacgcaaa tttgcgtaaa taaccggcaa atttacgtgc
     8341 cttttgtgag cttgctcgca cttcgccccg cgtcacccta cggcgatgcg aaggttaatt
     8401 cttacgaccg gtaatgcggc accactcttc tttttccacg accgggtcca gtgcgaagct
     8461 gtcgacataa gcttcacaaa cgctctctgc ctggcttgcc agaataccgg aaaggcccag
     8521 caaaccgcct gaaaccggca ggacgctgat taacggtgcc agttcacgta atgggcctgc
     8581 aaggatgtta gcgaccacca cgtcggcttt catttcttct ggctgatctt tcggtaagta
     8641 gagttccaga cggtcagaaa cgccattacg ttcggcgttg tcgcggctag cctgaatcgc
     8701 ctgcggatcg atatcaatac caatggcttt tgctgcaccc agtttcagcg ccgcgatagc
     8761 cagaatgccg gaaccacagc caaagtcgat gactatttta ccggttaaat cgaggctgtc
     8821 gagccactgc aggcacagag aggtagttgg atgggtaccc gtaccaaacg ccagccctgg
     8881 atctaacatc acgttgacgg cgttttcgtc cggcacatca cgccagctag ggcagatcca
     8941 cagtcgttca ccaaagcgca tcgggtggaa attatccatc cattcgcgct cccagtcttt
     9001 atcttccagt tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat ggttttccag
     9061 aatcgccacc acgtcgttca tatcggtttc agcgtcgaac agaccaatca catcggtgtc
     9121 gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt gggtatcctg
     9181 aaaagtgata gaaacggcac ccgcttccat cagcgcatcg ctaagatctt cagcgttcgc
     9241 gccggtggtg ttcagtttca gttggatcca aggcatggca aaactcttta tttatcagta
     9301 gtcaaaacgg tagcttgcgg gacggatgta ccgaaacggt ttccgaccag gaaagccagc
     9361 aaacttagta gtaacgaggg cacgataggg tggaagccca ggtactgaat attcagcgtc
     9421 gcgagtacgg catacagcac gccgccaacg atcatcgcac ttagcgcgcc tttggcgttg
     9481 gcgcgttccc agtaaagacc cagcaccagc ggccacagga aaacggcttc cagcccaccg
     9541 aaggccagca aattcagcca gatgatcatt tctggcggct tccaggcggc aagcagcagc
     9601 aacgcgccga gaactaacgt aattaccgcc gacatccgct tcagacgcgt ctcgttttgc
     9661 atttgatccg gacggatatt cagatagaga tctttaatga tcgtagcgga actttgtagc
     9721 aattgggcgt taattgtcga catgatcgca gccataggtg cagccagaaa gatcccggca
     9781 gcaaacggtg gcagcacttt taccattaac gtcgggatca ccagatccgg tacggtgaga
     9841 tcggggatca ctgcacgacc taacgctccg gctaggtgca taccgaacat caggattgcg
     9901 actacaatcg taccgatgat gatcccccgg tgtaccgctt tgctgtcttt ataagagata
     9961 cagcgcaccg cagtatgcgg caggccaatc acgccaaagc acaccagtac ccagaacgac
    10021 gtcataaagg caggcgacag aatatcgtca gcgccttgtg gcgtaaccag ttgcggatcg
    10081 atggtttgca aggtctgtac tgcgttgctt aagccgccag cggcatgtac cacgccaata
    10141 agcagcacaa cggtgccaat cagcatcaca agcccttgca tggtgtcgtt cagcacgctg
    10201 gcgcgaaagc cgccaaaggc ggtatacaac gcaatgctga taccgaaaat cagcagcccg
    10261 gtttcataag gaatacccgc cgcggtttcc agcaggcgcg caccgccgat aaactgcacg
    10321 gtcattgccc caacgaacgc aaccagcaaa ctcaaactcg ccagccacac cagaagacga
    10381 ctctggtagc gggcaaacag catgtcgttc agcgtcactg cattgtagcg gcgcgcaaga
    10441 atcgcaaact tcttgccgag aataccgagt gaaagccaga ctgcaggaag ctgaatcatc
    10501 gccagcaata cccagcccag cccgtattta taagcagctc ctggcccgcc gataaacgaa
    10561 ctggcactga tataggtcgc ggtgagcgtc atcgccagca caataccgcc catagagcgg
    10621 ctgccgagga aatactcatt aaggaaggtg ccggtgctcc gtttacgcat cgcataaacc
    10681 gagataccga acaccaccac cagataggcg accagcggta gaattacttc aagctgcatc
    10741 gtcatcctcc agtgggatat cgcgatagat aaatttcacc atcgcccagc acagtccaat
    10801 aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc cggtaaaacc
    10861 gggggcaacg ccaggtaagt aagcggctac taaccaaact gccagataca aaagggtcag
    10921 ccccagcgcc cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca ttttttgtcc
    10981 ctgatgggtg aagaaagcga ggattgtacc ttatgggggt tgtcgatccc cagtaataaa
    11041 aaaggccgga aaatccggcc ttttgacgct tttgcagtct tatttttcct gaagaccgag
    11101 ttttttctcc agatagtgga tgttagtgcc accatgctgg aagttctcgt cattcatgat
    11161 gcggatctgc agatcaacgt tggttttgat accgtcgatg atcagctcct gcagcgcatt
    11221 cttcatgcgg gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct taccgatcat
    11281 tgagtcatag tacggcggta cggtgtagcc cgcgtagata tgagactccc aacgtacgcc
    11341 aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg cttggcagga aggtgttcgg
    11401 atcttcggcg ttgatacgac attccaccgc atggccgcga acgtgaactt cttcttgctt
    11461 gatcgacagc ggttgaccgg cagcgatacg gagctgttct ttgatcaggt caacgccagt
    11521 gatcatttcg gtaaccgggt gttctacctg aatacgggtg ttcatttcga tgaaatagaa
    11581 ctcgccgttt tcgaacagaa actcgaaagt acctgcaccg cgatagccga tatcaacaca
    11641 cgctttagcg caacgttcgc cgatgtagcg acgcagttcc ggggtaatgc ccggtgctgg
    11701 cgcttcttcg accactttct ggtggcggcg ctgcatggag cagtcacgtt ccgccagata
    11761 gatagcgttg ccctgaccgt cagccagtac ctgaatctcg acgtggcgag gattttccag
    11821 gtatttctcc atgtaaacca tatcgttgct gaaagcagct ttcgcttccg cacgggtcat
    11881 ggagatggat tgtgccagtt cagcgtcgcc gcgcactacg cgcataccgc gaccaccgcc
    11941 gccgccggag gctttgataa tcaccggata accaatgcgt ttagcaatgg cacggttttt
    12001 atccatatcg tcgcccaacg ggccgtcaga acccggtacg caagggacgc ccgctttttt
    12061 catcgccgcg attgcggata ctttgtcgcc catcaggcga atggtttctg ctttcgggcc
    12121 gatgaagata aagccggagc gttcaacctg ctcggcaaag ttggcgttct cggagaggaa
    12181 gccgtaaccc ggatggattg ctactgcgcc ggtgatttca gcggcgctga tgattgccgg
    12241 gatgttcaga taacttttta ctgacggagc agggccaata cagaccgttt catctgccag
    12301 taatacgtgt tttagatcgc gatccgcgct ggagtgcaca gcgacagtct tgatgcccag
    12361 ttctttacag gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa taacaatttt
    12421 atccagcatg ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat tctaccggtt
    12481 gtccactttc gaccagaatt gctttcacgg taccggattt gtccgcttcg atctggttca
    12541 tcattttcat ggcttcaacg atgcacaggg tatcgcccac gttgactttc tgacccactt
    12601 cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc atcggggaac
    12661 gtacgatgtg accactgatt tccgctgctg ctggcgcttc catggaagga acggtcgccg
    12721 gagcggctgc gttagattga gctggctgct gcatcattgg tgcagcgtaa gcttgttgca
    12781 tcacagggaa acttgcggca ggagctgcac ggctaatgcg tactgactct tcgccttcag
    12841 aaatttccag ttcggagatg cctgattctt caaccagctc gatcagtttt ttaatcttac
    12901 gaatatccat gagtgggttc cgtactcttt gtttagtgtg attgtgacag gcgtttcacc
    12961 gccgtctgta aagcgtatgc cccgacagct gtatgcatag cgataaattc cagcaggccg
    13021 ggacgccggt ctattttgcc ccagagtatc aatgttagac ttgacggaca ttgtgcagcg
    13081 atctgctgct atcctccggc aaaaaacaaa atataccttc gttgcgccaa tgtcaccttt
    13141 tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca cattataacg atttcgtagc
    13201 aattggcagc taaatactgg tctcatcagg gaagataatc aacagctaac atgtaaataa
    13261 ccttcaacac cgtgtaattt gcaacaagcc gcacaattca cgaaattagc gccatcatcg
    13321 acggaacttc ttataacgta aggtgaaaag ctgtaaagcc agccctgcga agatgagggg
    13381 ctgcaatgag ataatcttca cagaccacaa ataatgtagg gatgccagga tcgcgacaag
    13441 atagacgaca ttacgcagcg gttgctgggg attacccaat tttcactgca tcgtctggat
    13501 agatgtgaat gctaaagcac gcaaaatcaa ccgactgaca attctcagca ttaaataggt
    13561 tcgagtggtt aatttgtagt caaataaaat cagattattc accccatggt accagaaatc
    13621 agtaacaggt taagcgtagt gtcgcccagg caaagcacca taaacctaac aggcgacgga
    13681 ggcgtatcaa taatggctgt ttagaggagt atcaatgcgc atcaatttgt ggtcagtcga
    13741 aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt gttcgacgaa
    13801 agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca aaatgagttc
    13861 cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc gaccctggat
    13921 ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata tctcttttgc
    13981 cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg cttgtgcgcg
    14041 gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac gcagaataaa
    14101 tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg ccacgcagcc
    14161 gccgtaattc atttgcgcca gcacttttgc cagcactttg tcgccaacag tgtcaattgc
    14221 cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac gagggagaat
    14281 acggctggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg aaacggcaac
    14341 gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc caccactggc
    14401 acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca gcgccatcac
    14461 acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg cgtccagccc
    14521 ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca gcccacccca
    14581 gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat gaaaacgcgg
    14641 atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg gaaaattacg
    14701 gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc tcgaccagtg
    14761 aacatcgacc gtgacatcgc cctccggcag gcgactttcg tccagagtct gtactgatgc
    14821 gagagttttg ccatcctgct gttctaaaag taacgcctgc ataagtggtc ctcatgtgca
    14881 tgatggattg gaaaataatt tctgaagatt atactcgcta atggaaaagc aaagccgatg
    14941 aagcgcaata aattgcgaga taaatctgat ttgctagtat gcccgcttcc tcactatcgg
    15001 agttaacaca aggatgagat taacgacgaa attttcggcc tttgttacgc tgctcaccgg
    15061 gttaacaatt tttgtgactt tgctgggctg ttcgctaagt ttctacaacg caattcagta
    15121 taagtttagt catcgcgttc aggcggtggc gacggcgatt gatacccacc ttgtgtcgaa
    15181 tgacttcagc acattaaggc cacaaattac cgaattaatg atgtcggcag atatcgttcg
    15241 tgtagacctg ctccatggtg ataaacaggt ttataccctg gccagaaatg gtagttatcg
    15301 tccggttggc tccagcgatc tgtttcgcga actgagtgtt ccgttgataa agcatccggg
    15361 gatgtcgctg cgtctggttt atcaggatcc gatgggcaac tatttccatt ctttaatgac
    15421 taccgcgccg ctcacggggg cgattggctt tatcattgtt atgctcttcc tggcggtacg
    15481 ctggttacaa cggcaacttg ccgggcaaga attgctggaa acccgggcta ctcgtatctt
    15541 aaacggtgag cgtggctcta atgtgttggg aaccatctat gaatggccgc ccagaaccag
    15601 cagtgcgcta gatacgctgc ttcgtgaaat tcagaacgca cgcgaacaac acagccgtct
    15661 tgatacgctg atccgctctt atgccgccca ggacgtgaaa accggcctca ataaccgact
    15721 ctttttcgat aatcagttag caacgttact ggaagatcag gagaaagtag gtacccacgg
    15781 gatcgtgatg atgattcgtc tgccggattt caatatgttg agcgatactt gggggcacag
    15841 ccaggttgaa gaacagttct tcactctgac taatctgctg tcgacattta tgatgcgcta
    15901 ccctggcgca ctgctggcgc gttaccaccg cagtgatttt gctgcgctgt taccgcaccg
    15961 gacgttaaaa gaggcagaga gcatcgccgg tcagttaatc aaagccgtcg ataccttgcc
    16021 gaacaataaa atgctcgatc gcgacgatat gatccacatt ggtatctgcg cctggcgtag
    16081 tggtcaggat accgagcagg taatggaaca tgcagagtct gccacgcgta atgcgggatt
    16141 gcagggcggc aatagctggg ctatttacga tgactcgttg cctgaaaaag gacgcggtaa
    16201 tgttcgctgg cgtacgctta tcgagcaaat gctcagtcgc ggcggcccgc gcctttatca
    16261 aaaaccggcg gttactcgcg aaggtcaggt tcatcatcgc gaactcatgt gccgcatctt
    16321 cgatggtaat gaagaggtta gctcggcgga gtatatgccg atggtcttgc agtttggctt
    16381 atcggaagag tatgaccgtc tgcaaatcag ccgtcttatt ccactattgc gttactggcc
    16441 agaggaaaat ctggcgattc aggttaccgt tgagtcgctg attcgcccgc gttttcagcg
    16501 ttggctgcgc gatacgttaa tgcaatgtga aaaatcacaa cgaaaacgca taattattga
    16561 acttgcagag gccgatgtag gtcaacatat cagtcgttta caacctgtta ttcgtttagt
    16621 gaatgcttta ggggtacggg tagccgtcaa ccaggctggt ttgacgctgg taagcaccag
    16681 ttggatcaaa gaacttaatg ttgagttact caagctccat ccggggctgg tcagaaacat
    16741 tgagaagcga acggagaacc agctgctggt tcaaagcctg gtggaagcct gctccgggac
    16801 cagcacccac gtttacgcca ccggcgtgcg ttcgcgaagc gagtggcaga ccctgattca
    16861 gcgcggtgtt acaggcgggc aaggggattt tttcgcgtcc tcacagccac ttgatactaa
    16921 cgtgaaaaaa tattcacaaa gatactcggt ttaacctgcc gtttaatccg ttttcacgta
    16981 gaataacgcg cgctgcgtct catgggagtg tgcttgtctg ctcgccagat tgttgcagca
    17041 catatgcaga tgaatgacct tacgcggttg caaacaggcg aggaatgctg ctgatgcatt
    17101 aagcctttct ggactcaggc agagatttgt aacaaaggaa acgaactgca ctaattttca
    17161 ccgtagcaga tgatttttgc gccttgtcgc tgctgcgtgt ggttggtaaa gtaagcggat
    17221 tttcttttcc gccccagctt tcaggattat cccttagtat gttgaaaaaa tttcgtggca
    17281 tgttttccaa tgacttgtcc attgacctgg gtactgcgaa taccctcatt tatgtaaaag
    17341 gacaaggcat cgtattgaat gagccttccg tggtggccat tcgtcaggat cgtgccggtt
    17401 caccgaaaag cgtagctgca gtaggtcatg acgcgaagca gatgctgggc cgtacgccgg
    17461 gcaatattgc tgccattcgc ccaatgaaag acggcgttat cgccgacttc ttcgtgactg
    17521 aaaaaatgct ccagcacttc atcaaacaag tgcacagcaa cagctttatg cgtccaagcc
    17581 cgcgcgttct ggtttgtgta ccggttggcg cgacccaggt tgaacgccgc gcaattcgtg
    17641 aatccgcgca gggcgctggt gcccgtgaag tcttcctgat tgaagaaccg atggctgccg
    17701 caattggtgc tggcctgccg gtttctgaag cgaccggttc tatggtggtt gatatcggtg
    17761 gtggtaccac tgaagttgct gttatctcct tgaacggcgt ggtttactcc tcttccgtgc
    17821 gcattggtgg tgatcgtttc gacgaagcta tcatcaacta tgtgcgtcgt aattacggtt
    17881 ctctgatcgg tgaagccacc gcagaacgta tcaagcacga aatcggttcg gcttacccgg
    17941 gcgatgaagt ccgtgaaatc gaagttcgtg gccgtaacct ggcagaaggt gttccacgcg
    18001 gttttaccct gaactccaac gaaatcctcg aagcactgca ggaaccgctg accggtattg
    18061 tgagcgcggt aatggttgca ctggaacagt gcccgccgga actggcttcc gacatctccg
    18121 agcgcggcat ggtgctcacc ggtggtggcg cactgctgcg taaccttgac cgtttgttaa
    18181 tggaagaaac cggcattcca gtagttgttg ctgaagaccc gctgacctgt gtggcgcgcg
    18241 gtggcggcaa agcgctggaa atgatcgaca tgcacggcgg cgacctgttc agcgaagagt
    18301 aatcggatgc aggcagggga aatgtctgtt taccctgcct ggtctgatac gagaatacgc
    18361 ataacttatg aagccaattt ttagccgtgg cccgtcgcta cagattcgcc ttattctggc
    18421 ggtgctggtg gcgctcggca ttattattgc cgacagccgc ctggggacgt tcagtcaaat
    18481 ccgtacttat atggataccg ccgtcagtcc tttctacttt gtttccaatg ctcctcgtga
    18541 attgctggat ggcgtatcgc agacgctggc ctcgcgtgac caactagaac ttgaaaaccg
    18601 ggcgttacgt caggaactgt tgctgaaaaa cagtgaactg ctgatgcttg gacaatacaa
    18661 acaggagaac gcgcgtctgc gcgagctgct gggttccccg ctgcgtcagg atgagcagaa
    18721 aatggtgact caggttatct ccacggttaa cgatccttat agcgatcaag ttgttatcga
    18781 taaaggtagc gttaatggcg tttatgaagg ccagccggtc atcagcgaca aaggtgttgt
    18841 tggtcaggtg gtggccgtcg ctaaactgac cagtcgcgtg ctgctgattt gtgatgcgac
    18901 ccacgcgctg ccaatccagg tgctgcgcaa cgatatccgc gtaattgcag ccggtaacgg
    18961 ttgtacggat gatttgcagc ttgagcatct gccagcgaat acggatattc gtgttggtga
    19021 tgtgctggtg acctccggtc tgggcggtcg tttcccggaa ggctatccgg tcgcggttgt
    19081 ctcttccgta aaactcgata cccagcgcgc ttatactgtg attcaggcgc gtccgactgc
    19141 aggactgcaa cgtttgcgtt atctgctgct gctgtggggc gcggatcgta acggcgctaa
    19201 cccgatgacg ccggaagagg tgcatcgtgt tgctaatgaa cgtctgatgc agatgatgcc
    19261 gcaggtattg ccttcgccag acgcgatggg gccaaagtta cctgaaccgg caacggggat
    19321 cgctcagccg actccgcagc aaccgacgac aggaaatgca gctactgcgc ctgctgcgcc
    19381 cacacagcct gctgctaatc gctctccaca aagggctacg ccgccgcaaa gtggtgctca
    19441 accgcctgcg cgtgcgccgg gagggcaata gtggcgagct atcgtagcca gggacgttgg
    19501 gtaatctggc tctctttcct cattgcgctg ttgctgcaaa tcatgccctg gccggataac
    19561 ctgattgttt tccggccaaa ctgggtgtta ctcatcttgt tgtattggat cctggccttg
    19621 cctcatcgcg taaatgtggg cacaggtttt gtgatgggtg ccatactgga tctgatcagc
    19681 ggctcgacgc ttggcgtacg cgtattggcg atgagcatca ttgcttacct ggtggcgctg
    19741 aaataccagc ttttccgcaa cctcgcatta tggcagcagg cgctggtcgt catgttgctt
    19801 tcgctggtgg tggatattat tgttttctgg gcagagtttt tagtgattaa cgtctctttc
    19861 agaccggaag tgttctggag tagtgtagtc aatggggtgc tctggccgtg gattttcttg
    19921 ctgatgcgta aagtccgtca gcagtttgca gtgcaataaa ggtttctatg acttctctgt
    19981 atttagcttc cggttctccg cgtcgtcagg agttacttgc gcaacttggt gtgacctttg
    20041 aacgtattgt tacgggcatt gaggagcagc gtcagccgca ggagagcgcg cagcagtatg
    20101 ttgtgcgtct ggcgcgcgag aaagcacagg cgggtgtcgc gcaaacggcg caggatctcc
    20161 cggtgctggg tgcggatact atcgttatcc tgaatggaga agtgctggag aaaccgcgcg
    20221 acgcagagca tgcggcgcag atgttgcgca aattatcggg tcagacccat caggtgatga
    20281 cggcagtggc gttggccgac agccagcaca ttctcgattg cctggtggtc accgatgtga
    20341 ctttcagaac gttaacagac gaagacatcg cgggctatgt cgccagcggt gaaccgttag
    20401 ataaagcagg tgcatacggt attcaggggc tgggtggctg ttttgtcagg aagataaatg
    20461 gcagctatca cgccgtagtc ggcttacctc tggttgaaac gtatgaatta ttaagtaatt
    20521 ttaacgcact gcgtgagaaa agggataaac atgacggctg aattgttagt aaacgtaacg
    20581 ccttcggaaa cgcgagtggc gtatattgat ggcggtattc tgcaggaaat tcatattgaa
    20641 cgtgaggcgc gacgcggaat agtaggcaat atctacaagg gtcgtgtaag tcgtgtactt
    20701 ccgggtatgc aggcggcttt tgtagatatt gggctggata aagccgcgtt tcttcatgca
    20761 tccgatatca tgccgcacac cgaatgtgtg gcgggtgaag aacaaaagca attcacggtg
    20821 cgcgacatct cggaactggt tcgtcagggg caagatctga tggtgcaggt ggtgaaagat
    20881 ccgcttggca ctaaaggtgc gcgcctgacc accgatatca cgctgccttc tcgctatctg
    20941 gtctttatgc caggggcttc tcacgttggg gtttcccaac gtattgaaag cgaatcagaa
    21001 cgtgaacgcc tgaaaaaagt ggtcgcagag tattgcgacg agcagggcgg gtttatcatc
    21061 cgtaccgcag cggaaggggt tggcgaggct gaactggcct ccgatgccgc ttatctgaaa
    21121 cgcgtctgga ccaaagttat ggagcgtaaa aagcgcccgc agacccgtta tcagctgtac
    21181 ggcgaactgg cgctggctca gcgtgttctg cgtgatttcg ccgacgccga actggaccgc
    21241 attcgcgttg actcacgcct gacttacgaa gcattacttg agttcacctc ggagtacatt
    21301 cccgagatga ccagcaagct ggagcattac accggacgcc agccgatttt cgatctcttt
    21361 gatgtcgaaa acgaaatcca gcgagcgctg gaacgcaaag tagaactgaa atccggtggt
    21421 tatctcatta tcgaccagac cgaagcgatg accaccgtgg acatcaatac cggagcgttt
    21481 gtcggtcatc gcaatctgga cgacaccatt ttcaatacca atattgaagc gacgcaggct
    21541 atcgctcgcc agttacggtt gcgtaatctg ggcgggatta tcattattga tttcatcgat
    21601 atgaataatg aagatcaccg ccgccgcgtg ctgcactcgc tggagcaggc gttgagcaaa
    21661 gaccgggtga aaaccagcgt taatggtttt tcggcgctgg ggctggtgga gatgacgcgt
    21721 aaacgcaccc gcgaaagcat tgagcacgta ctgtgtaacg aatgcccaac ctgccacggt
    21781 cgcggaacgg tgaaaaccgt ggaaacggta tgctatgaaa tcatgcgcga gattgttcgt
    21841 gtccaccatg cttacgactc cgaccgtttc ctggtctatg cttctccggc agtagccgaa
    21901 gccttgaaag gcgaagagtc acactcgctg gcggaagtgg aaattttcgt tggcaaacag
    21961 gttaaagtac aaattgaacc gctctataac caggagcagt ttgacgtcgt aatgatgtaa
    22021 acagatgctg ggccgccatc cggcaaaggg tttttgagtc acatttttag cagacaagga
    22081 gtgacgggtg aggcgattgc cggggatttt actgcttact ggagccgcgc tcgttgtgat
    22141 cgctgccctg ctggttagcg gcctgcgtat tgctttaccg catcttgacg cctggcgtcc
    22201 ggaaatcctc aacaaaatag aatccgcgac tggcatgccg gtagaagcca gtcagctctc
    22261 agccagctgg cagaattttg gcccgacgct tgaagcacac gacatccgtg cagaactaaa
    22321 agatggcggc gaattttcgg ttaaacgcgt tactctggcg ctggatgtct ggcagagcct
    22381 gttacatatg cgctggcagt ttcgcgacct cactttctgg cagctgcgct ttcgcaccaa
    22441 cactcctatc accagcggtg gtagcgatga cagtctggaa gccagtcaca tcagcgatct
    22501 gtttcttcgt caatttgacc atttcgatct ccgcgacagt gaagtcagtt tcctgacgcc
    22561 atccggtcag cgtgccgagc tggcgatccc acaactcacc tggctgaacg atccacgtcg
    22621 acaccgtgcg gaaggcctgg taagcctctc cagccttacc ggacagcacg gcgtgatgca
    22681 ggtgcgcatg gatttgcgcg atgatgaggg gttgttaagc aatggtcgcg tctggctaca
    22741 ggcggatgac atcgacctga agccgtggct cggtaaatgg atgcaggaca atattgctct
    22801 ggaaacggca cagttctccc ttgaaggctg gatgacgatc gacaaaggcg atgtaaccgg
    22861 cggtgacgtc tggctgaaac agggcggtgc cagctggttg ggcgagaagc aaacgcatac
    22921 gctgtcggtg gataatctga ccgcgcatat tacgcgtgaa aatccgggct ggcagttctc
    22981 tattcccgat acacggatca cgatggacgg caaaccctgg ccgagcggag cattgacgct
    23041 ggcctggata ccggaacagg acgttggcgg caaagacaat aaacgcagtg acgaactccg
    23101 gattcgcgcc agtaatctgg agctggcagg cctggagggc atacgcccgc tggccgcgaa
    23161 actttcacct gcactgggtg atgtttggcg ctccacacaa ccgagcggca agattaacac
    23221 tctggcgctg gatatcccgc ttcaggcggc agacaagacc cgttttcagg catcgtggag
    23281 cgatctggcc tggaagcaat ggaaattatt accgggtgcg gaacacttct ccgggacgct
    23341 ttccggcagc gttgaaaatg gtttgcttac cgcgtcgatg aagcaggcaa agatgcctta
    23401 cgaaacggta ttccgtgcgc cactggaaat cgccgacggc caggcaacta taagctggct
    23461 gaacaatgac aaaggtttcc agctggatgg gcgtaatatt gacgttaaag ccaaagccgt
    23521 ccatgcgcgc ggcggttttc gttacctgca acctgctaac gatgaaccct ggctgggtat
    23581 tctggctggc atcagtaccg atgatggttc acaagcctgg cactatttcc cggaaaactt
    23641 gatgggtaaa gacctggttg attacttaag tggcgcgatt cagggcggtg aagcggataa
    23701 cgcgacgctg gtttatggtg gcaatccgca actcttcccc tataaacaca acgaaggtca
    23761 gtttgaagtg ctggtgccgc tgcgcaacgc gaagtttgcc ttccagccgg actggcctgc
    23821 attaactaac cttgatattg aactggactt tattaacgac ggtttatgga tgaaaaccga
    23881 tggcgttaat ctgggcggcg tgcgcgcgag taatctcacc gcagtgatcc ctgactactc
    23941 gaaagaaaaa ctgctgattg acgctgacat taaaggtccg ggtaaagccg ttggccctta
    24001 ctttgatgag acaccgctga aagattctct gggtgcgacc ctgcaagaac tccagctcga
    24061 cggcgatgtg aatgctcgct tacatcttga tatcccgctg aacggcgagc tggtaaccgc
    24121 gaaaggtgaa gtgacgctgc gtaataacag tctgtttatc aaaccactcg acagcaccct
    24181 gaaaaatttg agcggtaaat tcagctttat caatggcgat ctgcaaagtg aaccactgac
    24241 agcaagctgg tttaatcagc cgttgaacgt ggatttttcc accaaagaag gggcaaaagc
    24301 ctaccaggtt gcggtgaatc tcaacggtaa ctggcaaccg gcgaaaaccg gcgttctgcc
    24361 tgaagcggtg aacgaagcat tgagtggcag cgtggcgtgg gatggtaaag tgggcattga
    24421 tctgccttat catgctggcg cgacctataa cgtagagctg aacggcgatc tgaataatgt
    24481 gagtagtcac ttaccttcac cgttagccaa acctgcgggt gaaccactag cggtaaacgt
    24541 taaggttgat ggcaatctca acagctttga attaaccgga caggctggtg cggataacca
    24601 tttcaatagc cgctggttgc ttggtcaaaa gctgacgctc gatcgtgcta tttgggcggc
    24661 agacagtaaa acgctcccgc cgttgccgga acaaagtggc gttgaactca atatgccgcc
    24721 gatgaatggt gccgagtggc tggccctgtt ccagaaaggc gctgcggaga gtgtcggtgg
    24781 tgcagcgagt ttcccacaat acataacgtt acgtacgcct atgttgtcac tgggaaatca
    24841 gcaatggaat aacctgagta ttgtttcgca accgacggca aatggcaccc tggttgaggc
    24901 gcaagggcgt gaaatcaacg ccacgctagc gatgcgtaat aacgcgccgt ggctggcgaa
    24961 tatcaaatat ctttattaca acccgagcgt ggcgaaaact cgtggtgatt caacgccgtc
    25021 atcacctttc ccgacaacgg agcgcattaa cttccgtggc tggccggacg cacaaatacg
    25081 atgcacagag tgctggttct gggggcaaaa attcggtcgc attgacagtg atatcaccat
    25141 ttctggcgat acgttaacgc tgaccaatgg actgattgat actggtttct cgcggcttac
    25201 tgccgatggt gaatgggtta ataatccggg gaatgaacgt acctcgctga aaggaaaact
    25261 gcgcgggcag aaaattgatg ccgccgcaga attttttggt gtcacgacgc ccatacgcca
    25321 gtcgtcattt aatgtggatt acgatttaca ctggcgcaaa gcaccctggc agccagatga
    25381 agcgacgttg aatggcatca ttcatactca actgggtaaa ggcgaaatta ccgaaatcaa
    25441 taccggacat gccgggcaat tgctgcgctt attgagcgta gatgccctga tgcgtaagct
    25501 gcgttttgat ttcagagaca cttttggcga agggttctat tttgactcca ttcgcagcac
    25561 cgcgtggatt aaagacgggg ttatgcacac cgacgacacg ctggtggatg gcctggaggc
    25621 ggatatcgcc atgaaagggt cggtaaatct ggtacgtcgc gacctgaata tggaagcggt
    25681 tgtcgcacca gagatttctg cgacggtggg cgtggctgcg gcttttgcgg ttaaccccat
    25741 tgttggcgcg gcagtttttg ccgctagtaa agtgctgggg ccgctgtgga gcaaagtctc
    25801 cattttgcgc tatcacattt cgggtccgct ggacgatccg caaatcaacg aagtgttgcg
    25861 ccaaccgcgt aaagaaaaag cgcaatgatt tgacgagggc gcgtaattgc cccaatctca
    25921 tatgataatc gttgccaaag gccaacgagc cagaacataa ccgtaggccg gatagggcgt
    25981 tcatgccgca tccggcagcc gtaaaaaatc ctctactgca gtaactaacg agtagcaaaa
    26041 acgatgagtc tgaacctggt aagtgaacaa ttgctagcgg cgaacagcct gaaacatcag
    26101 gacttgttcg cgatcctcgg tcaactggcc gaacgtcgcc ttgattatgg cgatctctat
    26161 tttcagtcga gctatcacga atcctgggtt ttagaagacc gcattattaa agatggttct
    26221 tacaacatcg atcagggcgt tggcgtgcgt gcaatcagcg gtgaaaaaac cggatttgct
    26281 tacgctgacc aaatcagcct gctggcgctg gaacagagtg cgcaagcggc gcgcaccatc
    26341 gtccgtgata gtggtgatgg caaagtacag acgctgggcg cggtagagca tagcccgttg
    26401 tatacctcgg tagatccgct gcaaagcatg agccgtgaag agaagctgga tatcctgcgt
    26461 cgcgtcgata aggttgctcg cgaagcggac aagcgcgtac aggaagtgac tgccagcctc
    26521 agtggtgtct atgaattaat tctggttgcg gccaccgacg gcacgctagc ggcggatgtc
    26581 cgtccgctgg tgcgtctttc cgtgagcgtt ctggtcgaag aagatggcaa acgcgaacgc
    26641 ggtgccagtg gcggcggcgg tcgttttggt tatgagttct tccttgccga tcttgacggc
    26701 gaagtccgtg cggatgcatg ggcaaaagaa gcagtgcgta tggcgctggt caatctttct
    26761 gccgttgctg caccagcggg caccatgccg gtagtacttg gcgcaggttg gccgggcgtg
    26821 ctgttgcatg aagcggtagg tcacggtctg gaaggcgact tcaaccgccg cggcacttca
    26881 gtatttagtg ggcaggtcgg ggagctggtg gcttcagaac tgtgtaccgt ggttgatgac
    26941 ggcacgatgg tcgatcgccg tggttcggtg gcgattgatg acgaaggtac gccaggccag
    27001 tacaacgtgc tgattgagaa cggcattctg aaaggctaca tgcaggataa actcaacgcg
    27061 cgtttgatgg ggatgacgcc gactggcaac ggtcgccgtg aatcctacgc ccatctgccc
    27121 atgccgcgta tgactaacac ctatatgctg ccgggtaaat cgaccccgca ggaaattatt
    27181 gaatccgttg agtacggtat ctatgcaccg aactttggtg gcggtcaggt ggatatcacc
    27241 tccggcaaat tcgttttctc cacttcagaa gcatatctga ttgaaaacgg taaagtaacg
    27301 aagccggtga aaggcgcaac gttgattggt tccggtatcg aaaccatgca gcagatttcg
    27361 atggttggca acgacctgaa actggataac ggcgtgggtg tctgcggtaa agaagggcaa
    27421 agtttgccgg ttggcgtggg ccagccaacg ctgaaagttg ataacctgac tgttggcggt
    27481 actgcgtaat aatttcatta ttttcagaag gataattaat ctttctacgt gcacgaacgg
    27541 tcccctcgct cctctggggt tagggtgagg ggaacccgtt ggcacaggtt tgtagaacgt
    27601 aacagtacaa tatgaattac ttctctttcc cgcgcccgtg catctcctga aacaatttac
    27661 cgacctcaac aaaataatcc gtcagcgagt tgatcacgac ctgtaccttc agcggcagct
    27721 tatctttttc ggtatataac gcataaaccg ggcgtggatc tgactgataa cgcggcagca
    27781 ggatctccag ctcaccgcga ttgatctcgt tgatcaccca catcagcggc acgtaggcga
    27841 tcccggcacc cgccgtcagc cagcgcacca gcgtcatcgg atcattagtc acaaatcttc
    27901 cttgtgggat caggcgagtc gagatccctt ccggtgcgat cagttcaaat tcattgtcgg
    27961 gccgcacgct gtattcaagc catgaatgac tactcaaatc ggcgggtttt tccggtatgc
    28021 cgtattgtgt gagatagctt ttcgcggcgc acaccaccat cggcatcgcg cccagacggc
    28081 gggaaaacag gctggaatcc tgcaacgcgc cgacgcggat caccacatcc agaccgtcgg
    28141 caatcaggtc gggggctgga attccggtaa ccagattgac gctcaaacct gggtattctt
    28201 tcagcatttt tgctgtcagc ccggcgagaa cattttgtgc catagttgaa gaacagccaa
    28261 tgcgcagcgt cccgatgggg gtgttattga aggcatacag ttgctcatga acatcctgca
    28321 cttcatgaag catccgacgg cagccctggt agtaaattct gccggcttcg gtcaggccaa
    28381 tgctgcgtgt gctacggttt aacagcttta cctgcaactc atcttccagt tttgacaccg
    28441 tctgactgat ggacgaaacg ctcatctgta gctgtctggc ggcggcggta aaagagccaa
    28501 attcaactac tttggcaaac accgacatgc gttttagtcg ttccattatt cactctgact
    28561 taaaagtgat ttagatcaca taatatagat aacagcataa cagttacgct aatatattaa
    28621 atatcaatct acagcaatgt tgctctcgcc cggctttcca tgcccttctc tgcggcgaca
    28681 gatgctgaaa ataataacgc ctgctctctc tttacaacca aggtcaacat gagtctgttt
    28741 cccgttatcg tggtgtttgg actgtccttc ccaccgatat tttttgaatt gcttttatca
    28801 ctggcgattt tctggctggt gcgccgggta cttgtgccaa caggtatcta cgactttgtc
    28861 tggcatccgg cgttgttcaa caccgcgctc tattgctgct tgttttattt gatatcgcga
    28921 ctgttcgttt gaggttgaag tgaaaacact aataagaaaa ttctcccgta cggccatcac
    28981 ggtcgtatta gtcattctag ccttcatcgc aatttttaat gcctgggtct attacaccga
    29041 atccccctgg acgcgtgacg cgcgctttag cgctgacgtc gttgcgatcg cgccggacgt
    29101 ttctggactc attacccagg tgaatgttca tgataaccag ctggtgaaaa aaggacagat
    29161 actgttcacc atcgaccagc cgcgctatca aaaggcgctt gaggaagcgc aagccgatgt
    29221 tgcttattat caggtactgg cacaggagaa acgccaggag gccggacgtc gtaaccgtct
    29281 cggtgtgcag gcgatgtctc gcgaagagat cgaccaggcc aacaacgtac tacaaacggt
    29341 tctgcatcag ttagcgaaag cgcaggcgac ccgcgatctg gcaaaactgg atcttgaacg
    29401 cacggtgatc cgcgcgccag cagatggctg ggtgaccaac ctcaacgtct ataccggtga
    29461 gtttattact tgaggatcaa cggcggttgc gctggtgaaa cagaactcct tctatgtact
    29521 ggcctatatg gaagaaacta agctggaagg ggggcgtccg gggcatcgtg cagagatcac
    29581 gccgcttggc agtaacaaag tgctgaaagg gactgttgat agtgttgccg caggggtcac
    29641 caacgccagc agcacgcgtg acgacaaagg gatggcgact atagactcta atcttgaatg
    29701 ggtgcgtctt gcgcaacgtg ttccggttcg tattcgtctc gacaaccagc aagagaacat
    29761 ctggcctgcg ggcaccactg ctacagtggt ggtcactggc aaacaagatc gcgacgaaag
    29821 ccaggattcg ttcttccgta aaatggccca tcgcctgcgt gagtttggtt aatcacgatg
    29881 ggtattttct ccattgctaa ccaacatatt cgctttgcgg taaaactggc gaccgccatt
    29941 gtactggcgc tgtttgttgg ctttcacttc cagctggaaa cgccacgctg ggcggtactg
    30001 acagcggcga ttgttgccgc cggtccggcc tttgctgcgg gaggtgaacc gtattctggc
    30061 gctattcgct atcgtggctt tttgcgcatc atcggcacat ttattggctg tattgccgga
    30121 ctggtgatca tcattgcgat gatccgcgca ccattattga tgattctggt gtgctgtatc
    30181 tgggccggtt tttgtacctg gatatcctcg ctggtacgaa tagaaaactc gtacgcgtgg
    30241 gggctggccg gttataccgc gctgatcatt gtgatcacca ttcagccgga accattgctt
    30301 acgccgcagt ttgccgtcga acgttgtagc gagatcgtta tcggtattgt gtgtgcgatt
    30361 atggcggatt tgctcttttc tccgcgatcg atcaaacaag aagtagatcg agagctggaa
    30421 agtttgctgg tcgcgcaata tcaattaatg caactctgta tcaagcatgg cgatggtgaa
    30481 gttgtcgata aagcctgggg cgacctggtg cgacgcacca cggcactaca aggcatgcgc
    30541 agcaacctga atatggaatc ttcccgctgg gcgcgggcca atcgacgttt aaaagcgatc
    30601 aatacgctat cgctgacgct gattacccaa tcctgcgaaa cttatcttat tcagaatacg
    30661 cgcccggaat tgatcactga tactttccgc gaattttttg acacgccggt agaaaccgcg
    30721 caggacgtcc acaagcagct caaacgcctg cggagagtta tcgcctggac cggggaacgg
    30781 gaaacgcctg tcaccattta tagctgggtc gcggcggcaa cgcgttatca gcttctcaag
    30841 cgcggcgtta tcagtaacac aaaaatcaac gccaccgaag aagagatcct gcaaggcgaa
    30901 ccggaagtaa aagtagagtc agccgaacgt catcatgcaa tggttaactt ctggcgaacc
    30961 acactttcct gcattctggg cacgcttttc tggctgtgga cgggctggac ttccggcagt
    31021 ggtgcaatgg tgatgattgc ggtcgtgacg tcactggcaa tgcgtttgcc gaatccacgc
    31081 atggtggcga tcgactttat ctatgggacg ctggccgcgc tgccgttagg gctgctctac
    31141 tttttggtga ttatccctaa tacccaacag agcatgttgc tgctgtgtat tagcctggca
    31201 gtgctgggat tcttcctcgg tatagaagta cagaaacggc gactgggctc gatgggggca
    31261 ctggccagca ccataaatat tatcgtgctg gataacccga tgactttcca tttcagtcag
    31321 tttctcgaca gcgcattagg gcaaatcgtc ggctgtgtgc tcgcgttcac cgttattttg
    31381 ctggtgcggg ataaatcgcg cgacaggact ggacgtgtac tgcttaatca gtttgttttt
    31441 gccgctgttt ccgcgatgac taccaatgtg gcacgtcgta aagagaacca cctcccggca
    31501 ctttatcagc agctgttttt gctgatgaat aagttcccag gggatttgcc gaaatttcgc
    31561 ctggcgctga cgatgattat cgcgcaccag cgcctgcgtg atgcgccgat cccggttaac
    31621 gaggatttat cggcgtttca ccgacaaatg cgccgcacag cagaccatgt gatatctgcc
    31681 cgtagcgatg ataaacgtcg tcggtacttt ggccagttgc tggaagaact ggaaatctac
    31741 caggaaaagc tacgcatctg gcaagcgcca ccgcaggtga cggaaccggt tcatcggctg
    31801 gcggggatgc tccataagta tcaacatgcg ttgaccgata gttaagtcaa aaccgacgcc
    31861 aaaagcgtcg gttttttcat ggctatactt agcgatgcac ggcagaactc gccgcgaaac
    31921 gtgacggtgg caacagatga atatttatac ctttgatttt gatgagattg agagtcagga
    31981 ggatttttat cgtgacttta gccaaacctt tggtctggct aaagataagg tacgcgatct
    32041 cgactcacta tgggatgtgt taatgaacga tgtcctgccg ctaccacttg agattgaatt
    32101 tgttcatctg ggagagaaaa cgcgtcgccg ttttggcgcg ttaatattgc tgtttgatga
    32161 ggcagaggaa gagctggaag ggcatttgcg ttttaatgtt cgtcattagc gcaaaaaaaa
    32221 gcccccgaac cgggggcaat atcgtcggac aagacgatga gggtttattt gtacagttca
    32281 gccgtagcgt gccaggtgtc accgctacga gcttcagtaa tctggtaggc cgttgcgcct
    32341 ttctcttccg cttttttgtt cagcatttca cgcatatcca ttggcgaaga cgccacacca
    32401 cttacggata cggtcccgat tgcttcacga ttttgtgctt gtgcagtatc aatggagtcg
    32461 gcagcgaatg caccgaaaga gagaacagaa agtacgctta atgcagctac agtggttttg
    32521 attttcatga tttttacctc gacataatct tttagctggg cctttgtttc gtgacccata
    32581 tcacaaaatc aagcatacac taattattga actaattaat accacgctaa ctatttcagt
    32641 tgatactaag tgttaaaaat gtgaatttta ataacaaaaa agaattaaaa acacggttat
    32701 ttattgttaa ttctagtatt ttcaattaga taatgaattt tacataatgt gcttttttta
    32761 ccgtatgtat tcattgtgtg aatgatatgt cgcggtaaaa cgcactattc gttaaattaa
    32821 tcgcagggga aagcaggggg ttgagaggaa taagcaacat tttccccgcc gccagaagcg
    32881 acggggcaga gattaaagct cctggtcgaa cagctctaaa atcgcttcgt acaggtcttt
    32941 gacggtgaaa ccgttagcag gggtggtaaa gatggtgtca tcgccagcga tggtgcccag
    33001 aataccttct gctttgccca gtgagtccag caggcgagca attaactgcg ccgcgccagg
    33061 gctggtatga atcacgacaa ctgcatcgtt gtagtcgata tccagcacca gattcttcaa
    33121 tggactggag gtggttggta cacccagttc agctggcagg cagtaaacca tttccatttt
    33181 ggcattgcgt gtacgtacag caccaaactt ggtcaacatc cgcgagactt tagactgatt
    33241 aatattgtca aagccttgct cctgcaacgc ggcgacgatt tcgccctggg agctaaattt
    33301 ctcttcttta agtaatgctt taaatgcttt aactagttct tcttgcttag ccgagcttcg
    33361 cataagtcac ccgatatggt ggttgataca acattattgt gcatacagat gaatttttat
    33421 gcaaacagtc agccctgaag aaggctgaaa taatgttatg aaagagcggg attttatcaa
    33481 atttcgttat tgagaaacat gcctgcgtca cggcatgcaa attctgttta aaagtaaatt
    33541 aattgttatc aaattgatgt tgttttggct gaacggtagg gtatattgtc accacctgtt
    33601 ggaatgttgc gctaatgcat aagcgactgt taattacgta agttaggttc ctgattacgg
    33661 caattaaatg cataaacgct aaacttgcgt gactacacat tcttgagatg tggtcattgt
    33721 aaacggcaat tttgtggatt aaggtcgcgg cagcggagca acatatctta gtttatcaat
    33781 ataataagga gtttaggatg aaagtcgcag tcctcggcgc tgctggcggt attggccagg
    33841 cgcttgcact actgttaaaa acccaactgc cttcaggttc agaactctct ctgtatgata
    33901 tcgctccagt gactcccggt gtggctgtcg atctgagcca tatccctact gctgtgaaaa
    33961 tcaaaggttt ttctggtgaa gatgcgactc cggcgctgga aggcgcagat gtcgttctta
    34021 tctctgcagg tgtagcgcgt aaaccgggta tggatcgttc cgacctgttt aacgttaacg
    34081 ccggcatcgt gaaaaacctg gtacagcaag ttgcgaaaac ctgcccgaaa gcgtgcattg
    34141 gtattatcac taacccggtt aacaccacag ttgcgattgc tgctgaagtg ctgaaaaaag
    34201 ccggtgttta tgacaaaaac aaactgttcg gcgttaccac gctggacatc attcgttcca
    34261 acacttttgt tgcggaactg aaaggcaaac agccaggcga agttgaagtg ccggttattg
    34321 gcggtcactc tggtgttacc attctgccgc tgctgtcaca ggttcctggc gttagtttta
    34381 ccgagcagga agtggctgat ctgaccaaac gcatccagaa cgcgggtact gaagtggttg
    34441 aagcgaaagc cggtggcggg tctgcaaccc tgtctatggg ccaggcagct gcacgttttg
    34501 gtctgtctct ggttcgtgca ctgcagggcg aacaaggcgt tgtcgaatgt gcctacgttg
    34561 aaggcgatgg tcagtacgcc cgtttcttct ctcaaccgct gctgctgggt aaaaacggcg
    34621 tggaagagcg taaatctatc ggtaccctga gcgcatttga acagaacgcg ctggaaggta
    34681 tgctggatac actcaagaaa gatatcgcct tgggcgaaga gttcgttaat aagtaattga
    34741 ttagcgaata ataaaaaacc ggagcacaga c
//
LOCUS       DAZRVY010000033        34131 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZRVY010000033 DAZRVY010000000
VERSION     DAZRVY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.033"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHXOX_001626"
     CDS             38..580
                     /locus_tag="ACHXOX_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005118.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHXOX_001627"
     CDS             663..1364
                     /locus_tag="ACHXOX_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7005119.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHXOX_001628"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHXOX_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHXOX_001629"
     CDS             3979..5049
                     /locus_tag="ACHXOX_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005120.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHXOX_001630"
     CDS             5061..5603
                     /locus_tag="ACHXOX_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005121.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHXOX_001631"
     CDS             5611..6126
                     /locus_tag="ACHXOX_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005122.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHXOX_001632"
     CDS             6092..6829
                     /locus_tag="ACHXOX_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR7005123.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHXOX_001633"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHXOX_001633"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR7005124.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHXOX_001634"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHXOX_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR7005125.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHXOX_001635"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHXOX_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR7005126.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHXOX_001636"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHXOX_001636"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR7005127.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHXOX_001637"
     CDS             12136..14043
                     /locus_tag="ACHXOX_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7005128.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHXOX_001638"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHXOX_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR7005129.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHXOX_001639"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHXOX_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR7005130.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHXOX_001640"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHXOX_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR7005131.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHXOX_001641"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHXOX_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR7005132.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHXOX_001642"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHXOX_001642"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR7005133.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHXOX_001643"
     CDS             complement(18711..20471)
                     /locus_tag="ACHXOX_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7005134.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHXOX_001644"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHXOX_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR7005135.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHXOX_001645"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHXOX_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR7005136.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHXOX_001646"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHXOX_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR7005137.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHXOX_001647"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHXOX_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR7005138.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHXOX_001648"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHXOX_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR7005139.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHXOX_001649"
     CDS             complement(26072..26518)
                     /locus_tag="ACHXOX_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR7005140.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHXOX_001650"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHXOX_001650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR7005141.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHXOX_001651"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHXOX_001651"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR7005142.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHXOX_001652"
     CDS             complement(29281..29943)
                     /locus_tag="ACHXOX_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR7005143.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHXOX_001653"
     CDS             30116..30529
                     /locus_tag="ACHXOX_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR7005144.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHXOX_001654"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHXOX_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR7005145.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHXOX_001655"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHXOX_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHXOX_001656"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHXOX_001656"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR7005146.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHXOX_001657"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHXOX_001657"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR7005147.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHXOX_001658"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHXOX_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR7005148.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHXOX_001659"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHXOX_001659"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8829 a         8131 c         8897 g         8274 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctagcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca a
//
LOCUS       DAZRVY010000034        33163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZRVY010000034 DAZRVY010000000
VERSION     DAZRVY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.034"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1907
                     /locus_tag="ACHXOX_001660"
     CDS             <1..1907
                     /locus_tag="ACHXOX_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFR7005149.1"
                     /translation="KLISNKAKLKKILKNAAKYITPPPKLLPSEWCEANMVLVDGPQA
                     GDKVKLLSFQKGMIDAPFLENKKKYVLMTSAQIGKTTILNGILFNQMANDPCNMIIGQ
                     STAKEMSQYLAGKIRPSIEACDALKDVVTDKNDRNAVNNNNQLQLKTNHFLYMVSLTS
                     PSTLRGKTAKVGLLDEIDAATASEEGDPVALAANRLTTFGDEGRLVVSSTPTSKLGSI
                     NQQWLSSDMRMFFVPCPHCGRHQVIEWENVQFEWRNIDGKNLPDPDTARYICPHCKNA
                     WTEGERIRAVAQGEWRATRESEVAGFWISRLYSPFSTIRACVVDFSHAWQSFDLQSFY
                     NTVLGKVYDDQDTAVEANELEQLKTDVSIENIPDDVIFLCSGIDQQLDRAESTILGVA
                     KDKVYILDHRSFYDHNCERYESPVWDRLVNFKKTKFYNVNGERVPMLASFLDTSNGRF
                     TQAGYRICGKWKNLHAIKGSSSGNAPVIPVKPTRTGGHELLMLGVNVGKSAVREMLVR
                     NLKDNPHIGLEISETVPDDYLDQLLSESIKRTTTGVRWVKNPGSTRNEALDCLVYSYA
                     ASRYVLSKMSWDKLIAMKDSLNRVVEESVEAPKSQSDEQIEETKPITRPQRQNIARRP
                     NRGRSWVTSF"
     gene            1979..2473
                     /locus_tag="ACHXOX_001661"
     CDS             1979..2473
                     /locus_tag="ACHXOX_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005150.1"
                     /translation="MSLELIPLVIRKGEKITLANEEGVTIQVGNSKGIIYQVDDTPAN
                     HEIKTLDFAEGKYTIVITLEEELVSMQELTVLPVFTKQSKKDHLRETIATIEQVIFAR
                     LSGDEAALSQMTVKGNTFAYESLAVLQQLKTDYERQLSKLIQAERRKQGISPIKNIKL
                     RLTR"
     gene            2484..3995
                     /locus_tag="ACHXOX_001662"
     CDS             2484..3995
                     /locus_tag="ACHXOX_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7005151.1"
                     /translation="MFNLFRRKKAVETPVKTNHRQQQPKIFIDKQVEKFQKDLSKRSL
                     GLVGDRIDGSLQQDTITGTFNKALKSNGKRLYDQGRTLALNTSVGSRYTQYITDMVVG
                     TGLDPKPSVVKSNGKLDSALNKQIENAFWKWAQNAKRFSRNGRFNFRELLVMAERERV
                     MGGECFIVLTKENNELSVSILSADKCDWTLNREVSKERAIYQGIEYDVETMRPVAYWF
                     RKINLLTQTYTGDNYRVDASQVCHYYQPLAAESLRGVTDFLPVIKDIAHQDAFRETAI
                     VQKRIAASSMGFIERPKDSGDDFDTGEDDEQYQAPEVVQDFAPGTIQELPEGATIKSI
                     QATQSGDDFNSFNDAMFTSVSMGLGVFKQGLTGDTSQINYSAARFGELLQRNRVKSLQ
                     NKLIETVVLPIFEAYLRHYSARGIVPIRITAIPHIIENTTIIRPRFESVDVIKDVNAD
                     IALIDKGLKSRTAVILERGDDPEKVFSEIRSESSALNIIVNGEGEEKNSPADP"
     gene            4042..6048
                     /locus_tag="ACHXOX_001663"
     CDS             4042..6048
                     /locus_tag="ACHXOX_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR7005152.1"
                     /translation="MLKFRRDLNGYGGVINEGQNDQYEFEIAFSSEQPYQRRFWDEQN
                     QEMVVLDEILVHTPEAVDLSRLNNNAPLLFNHIFDNHIGVVCNARIDADNVGRALVKF
                     SKHGTLSNDIRNKVIEGTMEKISVGYDIKEYRIDYAKGQLIVTKWEPFEISFVTVPAD
                     DSVGLNRSLNTITVNLEAKRDMTKEQIEEIKEEQEPAQVEETPVEENKEPEVEKTQER
                     QVEENKEDENLEDGKDAKHPESVDDDSSTVREEEVKEEREAAPVEEEKTEEVAERSEE
                     DELEIREIARELNINEDDEEFKRALANKEITPEAFRTKALNKIATAQRNNEQIKDSKM
                     EKTFDLNNVIRSLVDGAALGANEAEYSAMAAGAAMQRGRAARGGSVFVPAAALRAASE
                     GNTKATLTAVTDEKLLTESYIEMLMPQSVLGRLGVTVLSGLNSPIAVPKMTASSVDAF
                     GFVDENGAAPESKAEFANVKMAPKTFAGGNPISRQSIKTVPGIATLITDHINKSVRLK
                     LEQLILSDKENARGPAGLVKQLVDAGRVTKKAAFSYKDFLKEIAALTDAGVPAQAIKF
                     AMSGATAAELESTLKDNGVSGYIIENGKLAGYEVVTSGVIPADHIVLGAFDGITIGEW
                     GGLELDMDDTTYRAQSAIVPRIWVDLDYVVTQPEALKVLHISAE"
     gene            6246..6536
                     /locus_tag="ACHXOX_001664"
     CDS             6246..6536
                     /locus_tag="ACHXOX_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005153.1"
                     /translation="MFKLSESQLSRMFRSAPVFSVEGGKSIRAYHEITTTDEQGVITE
                     SEFLFCREGDLNQGDVVIVDGERFKVQYVKRNGDNTSDCFIARAGGTHARYR"
     gene            6520..6942
                     /locus_tag="ACHXOX_001665"
     CDS             6520..6942
                     /locus_tag="ACHXOX_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005154.1"
                     /translation="MLATVNNMPRLKIKRALQDIIEQDLGLALNVEQTQQGFSDDVVC
                     WITGMNETYTRVRGGNAMQAECVIEMQLYSQNHETKIHEGICQIIQIQPDNPRFKDLG
                     FSISDITPVASNTDYDDDSSDGGIVGTLSLKFSYLARF"
     gene            6952..7785
                     /locus_tag="ACHXOX_001666"
     CDS             6952..7785
                     /locus_tag="ACHXOX_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005155.1"
                     /translation="MNITQDNLDIFTGSHVEVSISNQIDNQVDFFDPSFSSMENIAAF
                     PTLTESTEIETLEEYDQDATGKLAGYRRLEPTTLTLNRVLDDEHQAMLMKAVEDKTPL
                     RFRMFYVVNSGYSAANTGYFCIFDAYVTSHKTRGSDNKAVTLEFKLEPDGGILARGIA
                     TEGRILRQGDFGLGAGVHPFTGPIDSEALAGNRFVTYKGTASGNPYSTDTALIHLQAN
                     EHGAWQLTCNTSGAPRLRVRNIQENGRSEWIKVYSTNEKPTPSEIGAVAKTDRVDFGE
                     Y"
     gene            7862..11392
                     /locus_tag="ACHXOX_001667"
     CDS             7862..11392
                     /locus_tag="ACHXOX_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR7005156.1"
                     /translation="MQSIQFKRTQTAGKKPTPEQLSQGEIALQLADHVIYTKDKNNNV
                     VQISVSPEKHAELNTKVDNNKTSTDRVIASNKQEAADNLASAKAELNQTITSTRDTLN
                     ASITAGDTAANKRIDALTTTVGNNKTEAANALAAAKTELNQTITSTRDTINATINKNK
                     TDINARVDQTNTNVTNLTNTVAANKSAIEKTVADNKKDADSKITNLTSTVSANKTAIE
                     RTVANNKASADAAIAAANQRIDSIEGSADAAYIKKNTNTKHGGYLLSKTANYLEDQTA
                     RDLNYFGAFRTNGQDGLMDLTLNVPHSSGKAHGRGFTFRYASGGSRVETYGFDREGQK
                     NFSYKMYHEGDKPTPGEIGAYTKAEVDKMFVKNVVMSVPNSSENVSAYFKLATATIPQ
                     NGRSVFFRIHGGNGYNVTAYDQVDVVEILLRSGNDRPKGLNVIAYRRNTNKDFEVFAV
                     NTSGDNYDIYVKYQRYTDNVIVEYGKSVYVSLTVYDTPEATLIKPSVGVIGGRNVTLF
                     NTENKRGVLSFDDNTQNSYDIVHLSNDRGTGRKYIRKFRSNYNEMIWHETVQGSSYRL
                     ATGSTDAQEIMTIESSSSIAGTHKGNIISGRMLLNGGSNAITLRRPAGQSNHIAFQDN
                     RTGDITRQGWIGYANADTNVFEWYSDVGGTSIRHHIDGQIELATGRTKRVYTNAQFIS
                     MNSDAYRMVYGNYGAFWRNDGGKVYLLSTAENDKLGGWNGNRPFIYDLTNGKVTLGGD
                     GNEGALVLERDSRAARFSNNVFLEKGLLTFSAGGNQSMDSFTINHWGNSNANRYNVLQ
                     FQDTSGTHFTTERNGSGGLLAHFRGDLTTEGKLTWGKGTATSSFNIRAWGNSDSRKQV
                     FECVDESGWHWYTQRPGGPGTSTIEFAINGTVKPQAIHTGGNITLNGADIEFRRSGNK
                     HLWFRDPNGLELGLMYCDDAGVIRFRGQKQAQTWKLADKMIHLESGSVGGSDKGLIRG
                     NVSGGSWSSWRDRASGLQVDCPQSTDSAHNIWKATHWGKYHIAAMGVHVPSGTIGNAL
                     VRLHVNDTNFDFNAAGDFTAGRNGSFNDVYIRSDSRLKINKEELQDGALEKVNSLKVY
                     TYDKVKSLSDDTVIKREVGIIAQDLEKVLPEAVGIQSTEDPENPEAIKTISNSAVNAL
                     IIKAMQEMDAKYKAQIEALHKEIAELKATK"
     gene            11450..11923
                     /locus_tag="ACHXOX_001668"
     CDS             11450..11923
                     /locus_tag="ACHXOX_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR7005157.1"
                     /translation="MSQQFKDIFTGGLVSMFYHADTTNTALDHESYEEIKECAGFPET
                     GIERGTVEVKSFSSQYNRKLVGKLNVPDLTLTVNYIPGDAVHEKLIKAAEDGTRIQIK
                     IEYYVDAAKQTGIRTAFNGFISKVAMNGGDEEVVTKEFTFAVDGAPLKQQIFTAG"
     gene            12098..12604
                     /locus_tag="ACHXOX_001669"
     CDS             12098..12604
                     /locus_tag="ACHXOX_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005158.1"
                     /translation="MANVVKVPGWIGSSAVSVTGKRWMKEALTALKVPAPRNMSAMAG
                     RGMDTVVATASWSTSLGNNWGVTASNYPVSGMQSKGSMENPENVGVGRLVGVIVGQFN
                     GGTPTMAVYLQNGRAGNITVNLGGAAVTVPYNSMQSGFHYYWLSNPPAAFLTNIKKTG
                     TKQTLKIS"
     gene            12650..12958
                     /locus_tag="ACHXOX_001670"
     CDS             12650..12958
                     /locus_tag="ACHXOX_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome"
                     /protein_id="HFR7005159.1"
                     /translation="MNLNEMLKALSPKRESLTLGGFTFYARPMSVQEFNEHVFNTDKK
                     DRDERSILRCIEDEDGKPVFESIEQVKALYTSVRSELIGLVAQASLMKDAAVIENEVK
                     "
     gene            13003..13341
                     /locus_tag="ACHXOX_001671"
     CDS             13003..13341
                     /locus_tag="ACHXOX_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005160.1"
                     /translation="MSKDEMDTMPITLFWALHIFDTYLEPQSPLYQDARHAQSMYYMH
                     VTSPNMTREWLNKINVNQFRMIKDDKQFKTQEEIKEIARKKEEERNSALVDSYFDASL
                     LQKLKSGQLG"
     gene            13345..15687
                     /locus_tag="ACHXOX_001672"
     CDS             13345..15687
                     /locus_tag="ACHXOX_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005161.1"
                     /translation="MTRHIVTIEGNNDGLRRSTNQAIDMLDNLSERAMNLDLSGGLSS
                     LSGTLRGTGGSLALAAGGFVGLAAGAVSAANAIGDYVKEYSEVSKATGLTVETLQKLE
                     KQFSGTGLTVEKFGDINKDTLDKLADAWRNGGGIEDDLESVGLKIKDYAEFMNDPQGG
                     MKAAIKVFYDMRKAGASIADVKFMMESLASDSSHMADELQKYGSYQEAVNAINQQTVK
                     ITNDTAEEYNKFSKNMDTLTDNLKGMAANALNPVVEGMNKLYNWFDKDWEKTALFNAL
                     KTFNERFEKARQNMAMANGGFINKHGYSSTGLAGGQASFNNKPNYPDRWVDSEGVTRD
                     KNGDPVITITGNKSDTGFTPTGQGGGVTGPSKKEQEEAEKAAEEARKAAEKAQKQRED
                     AIKKLNALDVRLHGQTAASIASQNKQLEASLKDLDTALDMGLISQQDAAAKRQQLLDQ
                     NAENIYKMMLGADPVDALNALTVLQQIRDNELESHKRLLDGKAISYEEYMRRVNDTEQ
                     NYSQIDSHLQGMDGFKSNQLTSGFDYQDSNNPFAKFNAIDKEKSDAEQDYKTDKLKID
                     GINDPAKRMAALEKLNENHQKRMAAIEKKYADARQSIADDMYGGFAAAMTLFGQENTK
                     AMQMAFNAHKAFSIGQATVNMWTAATDAWNDPTNVTTGQKIAAAALAVSQNMGNIANI
                     KSTNVSGMAHDGIDNIPREGTWLLDKGERVVDQRTNGDLKDFLSAQKSGGGNSQPIEV
                     NAPLNINGNVNSSDKMVMDAIKRHAKLVAQAVEDAQRRKM"
     gene            15738..16298
                     /locus_tag="ACHXOX_001673"
     CDS             15738..16298
                     /locus_tag="ACHXOX_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005162.1"
                     /translation="MFKSKNIKITDFTLKSKQPFFKAQSISGKFQRRFTGIHFYEAEF
                     TANFMAQDINEVKEFVARHLFGRPFSVPLSYFSKYTGDVRQMVTAAAGTARGGRKVRI
                     SNFTGTLKAGTVIQFENHKKIYTITEDVKSGGEMKLFPNLRQNVLAGEVIKYQNVEGE
                     FVLKTENIDWKIAQIGKMKFELVENV"
     gene            16298..16951
                     /locus_tag="ACHXOX_001674"
     CDS             16298..16951
                     /locus_tag="ACHXOX_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005163.1"
                     /translation="MATIQESFSKLCTNLDFIEVYNDQTGQNVSRLTLPQLFSTGSMF
                     HIIEVITASGDVLRLTDGYFDLDYNGFTYLATGDFLQISSNTEEKEINNNGINVTVSN
                     VREEYITLIRNKQFDKSDVKIEMVFLNPNTGKVETTYPVFRGVVDSIGINIEHEDDEC
                     KNESEFQLNSIWEVLDKNARSHASDGIHRSYVGNENDLFFSRAGKWQSESKWFSSKK"
     gene            16998..17417
                     /locus_tag="ACHXOX_001675"
     CDS             16998..17417
                     /locus_tag="ACHXOX_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005164.1"
                     /translation="MLKTRLITDYINSLIGQEFVQGENDCNIIACKIIDILAGTDLYN
                     SLYKKYSTKEEGLKICKELSGYSNILQPIKKHFKLVTDDLQDGDLLVTAHKLGNRNYY
                     SVVPHYSGYGLVEEDGIWMTIPVSDIDYEQVYRFGGE"
     gene            17417..21193
                     /locus_tag="ACHXOX_001676"
     CDS             17417..21193
                     /locus_tag="ACHXOX_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFR7005165.1"
                     /translation="MGFEILVGAVIAGASAGMAAAATFSVMTAVAIGMAAGAMTLIAS
                     TVGAPKTPKVQSPDNAVTLGTSNDPKTVLPVLFGTTRTGAICVYKAISKQENNKLVQI
                     FAIAEGEIDHYKALFIDNKNVLVGKNMTIRDGVLDKGNIKEEYRKVLEVEFRTGKNPN
                     TALSLAKRHLGSDWNDNYKGNGIATMCIVLRRDDKSLAAGVDILQPNSQVAVDVMGLK
                     IRNLETNAIEASTNGVDQIFHYLTNEKYGLSVPIENINVDSFLKVRKQVRQMDLHSNG
                     ACDPNASFKENLTSLMQTFGGVMFESFGRITLKLDAPDIVKHTFNEDNIMMGKVSLKT
                     GGTNGYFNTINAMYQEPSIDYSEQMLRYPADAENDATIRQDGRIIAKDIEYRFVKSKA
                     QIDKLASVERNKSRITQVISFMTTDAFTAEVWDVISVTYDELKLNNSLWRITAIDRSI
                     DSGIAGMMTITATEYNSQVYTDLNYAATPDNRPSGLPDSMTVQKPTNFRIKATGETIY
                     GKNVTLTWDAPEDFNRYGFQIDYRVSGSPNWIKLGQTSQQIFSVNALAKDRSYDYRVC
                     AFGIIARSDWVELVNQNPTVTYELPTPVIRIKNQGSTPGTFEGNDLIIEWENQQQLDV
                     MINGEANKFSDLFEAYIIKVTNKAGKSIQYRTRDPESWTYTLDMNQFNGLSRQLTVEV
                     SAKGYNNSESAPARLVAINPQHKPMKGFSARGGFNTAFVSWADDVEHDYAGSIIQYAT
                     DNTFSDARAVSTNSVSHTSFDIADGDYYIRGAHYDIFGMDDAVWSEPYFMSMKSTISW
                     DDQDKEALEDLIGLQDRLDETIADAIAQAGANADAKIDAMHKQITTETGKTVQASADT
                     LKSLIVSGDQASSTKIDQVKAELKGDITKEVSASATTLKQAIATSEAASASKIDQVRV
                     EMDGKIAGVNQEADVKIDALKGTINSKYNLAVNADGRVAGIHMSATNDPAQPTKIIFT
                     ADKIAVAPQNGTDVCPFGIEGNKVYLDNAMIRNAAIGTAQINDAAITTAKIGNAAINS
                     AKIQDGAITNAKIVNGAIDNAKIGNYIQSSNWNGSTGWHINKNGSATFMNATVKGNIT
                     ADSGTLNNVTINSNCVIKGMLDATQVRGDFVKVIGRKFPVRDANVDNGYAGYPQGTVT
                     VRIEDDHKFDRQIVIPAISFGGLRAREGSNNNTFYDTCRLIVRKNGSELYNRAYGGNT
                     GLYSGVIDMPAGKGAVTLTFEVHSSAINNWTPSTWISDLTVMVTKKATTGITVS"
     gene            21300..21578
                     /locus_tag="ACHXOX_001677"
     CDS             21300..21578
                     /locus_tag="ACHXOX_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005166.1"
                     /translation="MTEIIYGGIGVIALICGGLWRLHRNQLATENRLSKLESNDALFN
                     QKFETMQNNHDQISERVYRMEQTLHGIEKKVVAMDAKFDQVIDLLRQK"
     gene            21598..21768
                     /locus_tag="ACHXOX_001678"
     CDS             21598..21768
                     /locus_tag="ACHXOX_001678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005167.1"
                     /translation="MKNKLKKYFGYLLIVALTYNVAIRPLLTSFGLELPAMTVDEQLL
                     RTLAGVFSLLGG"
     gene            21770..22453
                     /locus_tag="ACHXOX_001679"
     CDS             21770..22453
                     /locus_tag="ACHXOX_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005168.1"
                     /translation="MSSSANKRNALRTKKALRQWTDKATDTFEKAIGEGAIFAARALQ
                     KKINKNVDRPTRWTQQAVGNTNYKNRSGTRHQIFIKGARDKDKKIGSQDDYLKHYFDG
                     GKINKLVPIANGKVLDAHGNIKAIKGGKMMRNIENGNFIKVENQEGTFIMKKYKPKKS
                     RTKRAKNGSAVAKRRLEKRTQKQSKRIVAVKSDKISTRYSTMGSWESNEEMMLKNINK
                     HIKSRMRYV"
     gene            22492..22839
                     /locus_tag="ACHXOX_001680"
     CDS             22492..22839
                     /locus_tag="ACHXOX_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005169.1"
                     /translation="MAKNIFTEFPTYPVDQLSGIFINGISPESMTHDFEAKRVKHKQY
                     KECIRDHEKGTVFCVATLAKRPKYRFRVGQEVDVVNPYSFNCLGDARAVCVGTAPYYI
                     KGMRFIGYIFEMI"
     gene            22849..23202
                     /locus_tag="ACHXOX_001681"
     CDS             22849..23202
                     /locus_tag="ACHXOX_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HFR7005170.1"
                     /translation="MLSKHFSRKEFKCKCGKCDYDTIDAELLVILEDVREHFGKPVII
                     NSGNRCPTHNKNVGGAANSYHVRGRAADIVIKGVSPDIVHAYLDGKYPTQYGLGKYKT
                     FTHIDSRSKKSRWNG"
     gene            complement(23234..24724)
                     /locus_tag="ACHXOX_001682"
     CDS             complement(23234..24724)
                     /locus_tag="ACHXOX_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFR7005171.1"
                     /translation="MQDIKIHFQDYSFVKVDCSPSIGMELRDYFSFEVEGARFSPRYK
                     YSGWDGRIRLFTHENTLPIGLLKTLGVFVKNMGYSVWIDPRLLDKEDVTKDAINEWID
                     SLEVYSGSNKINPYWYQREAVFQGIHNRRRMLVLPTSAGKSLIACMLSRWYLEHYTGK
                     VLIIVPTTSLVVQLRDDFIDYRLFPYEAIHTMMSGSGKHPGDRLITVSTWQSACKMPP
                     EWFRQYGMLIVDESHKASAKNITNIINGMNHCQFKIGMTGSPKESKCHLMQYVGLFGD
                     ISKIVSIDRLMEEGQVTKLKINCLFLRYTDEECSAVKGREYAEEIKYITSNPRRNKFA
                     CNLALKLAKKGENVFLMFRNTKHGKLMYDALQKVHDKVYYIDGGVKTEERDEFKKMAE
                     GDTGIICVASYGVFSTGVSIKNLHHVIFGHPVKESTIVRQSIGRALRKHGSKDIATVW
                     DLIDHLCIFGRNGKVKHKNYAVKHALERIRYYLTDKFSYATKTIAI"
     gene            complement(24724..25281)
                     /locus_tag="ACHXOX_001683"
     CDS             complement(24724..25281)
                     /locus_tag="ACHXOX_001683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005172.1"
                     /translation="MAKDIVFTPKSSRAVTPSALEQRILTHIKEKDLPLVFHGFLGEF
                     KGLHSRIALTCTRCGNRADKSATNLLNEGKRCACLKSDYFQYKAGVPYLYVMKSGDIG
                     KFGVTKSIIKRRVNLGVRNPGHNFDIVFAGEMPSMKHALATERAIKRLLDVGIGGIEY
                     GKTETFHYCNKSLNNIKNIASVEWN"
     gene            25448..26623
                     /locus_tag="ACHXOX_001684"
     CDS             25448..26623
                     /locus_tag="ACHXOX_001684"
                     /inference="COORDINATES: protein motif:HMM:NF024847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFR7005173.1"
                     /translation="MKLAYAAEIPFVKKTRGLSPEEYQQRIIAKLESAFVFGGFILPW
                     KGNHTYFRIYNLDTQEYKDYKLRKLEDTNGGELFQTEKAVWLKMESRCNEKGKKFLGW
                     QGEWKGRNRTKARVLCPEHNQIMTPSLLHALKDDFDFDCKICMAEKSQRVRSGKTFDE
                     VIKDKETIINARCESTPYTFKGFIINTPHLKDVKFKTYCKTHNHEWESHLRCADSFTC
                     PLCIKDQLVQLSNRTYQGKASFYIQLLDDKFIKFGITTRKPEERMREQTRKSNFTHRL
                     IFTHEFDDGWKAVDLEHEVKQRFKTHAAPYKDFKDGWSETLTIDDLPHLQQLVYDYLT
                     NQPDEANMWVSPKDVFDDDTFKLHTHFYGINKPEFFCIDDDSPDLMDEYFNTLLDAA"
     gene            complement(26697..28376)
                     /locus_tag="ACHXOX_001685"
     CDS             complement(26697..28376)
                     /locus_tag="ACHXOX_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7005174.1"
                     /translation="MKTKLYSYIRFSSMRQSDGSSYERQIRMAREIAAKYDLELVNDY
                     QDLGVSAFKGANSKTGALSRFLDAIGRSVPVGSWLFIENLDRLSRADIVSAQELFLSI
                     IRRGITIVTGMDNKIYSLDTVTANPMDLMFSILLFIRGNEESQTKRNRTNSSALIKIK
                     AHQENPQNPAVAIEEVGKNMWWTDTTSGYVLPHPVYFPVVQEIVELRKNGRSTAEILD
                     HLNETYAPPPAVSNKKHSKWSRAMVERLFHTRALIGIKEVSVDGVNYELKDYYPRVID
                     DPTFYHLKKNIGARAWNFGDKEEAKPIPLLSGIGLLKCDHCGCAMVKVKGTNKRSDQY
                     RYTCDATRSRRLDCEHPNWSFRGDQLEKAVLQLLADKIWIAEDKANPVPALKVQVDEI
                     SRKIDNLIALSAMTGATKELADQITTLNSERETLYNQIKMAEEEMYSVDSQGWEKLAE
                     FDLEDVYNEDRIKVRFKIKQALKRIGCSRIDRNKNLFVLEYIDGKTQRVVIENSRGTR
                     KGQIFVDLKTINDRQILESNGLVLHPCLDMLTDKNWKPEEEIPGPLQEFGI"
     gene            complement(28430..28771)
                     /locus_tag="ACHXOX_001686"
     CDS             complement(28430..28771)
                     /locus_tag="ACHXOX_001686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005175.1"
                     /translation="MKKTIYAIIVSVCVGGIGGFVCGYDYSDTQHQETIRSTKAEIKD
                     LNQEISQLENALSLKDSEYSEVYADKQKYWKEKDNLVVYGMKLCKSMKDERSCLMELA
                     LKGNVTIDFKD"
     gene            complement(29073..29342)
                     /locus_tag="ACHXOX_001687"
     CDS             complement(29073..29342)
                     /locus_tag="ACHXOX_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005176.1"
                     /translation="MNITKAIHNYARRRGIELQLEDEQVRFWEIEQDCEWMFSYSIAQ
                     CGCLFWKGNVYLPRDIKEELPAMIGTDKKLKEVLDFIHKEFISKK"
     gene            29382..29723
                     /gene="ycdX"
                     /locus_tag="ACHXOX_001688"
                     /pseudo
     CDS             29382..29723
                     /gene="ycdX"
                     /locus_tag="ACHXOX_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
     gene            29747..30301
                     /gene="ycdY"
                     /locus_tag="ACHXOX_001689"
     CDS             29747..30301
                     /gene="ycdY"
                     /locus_tag="ACHXOX_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR7005177.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            30403..30894
                     /locus_tag="ACHXOX_001690"
     CDS             30403..30894
                     /locus_tag="ACHXOX_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR7005178.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(30959..31792)
                     /gene="csgG"
                     /locus_tag="ACHXOX_001691"
     CDS             complement(30959..31792)
                     /gene="csgG"
                     /locus_tag="ACHXOX_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR7005179.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(31819..32235)
                     /gene="csgF"
                     /locus_tag="ACHXOX_001692"
     CDS             complement(31819..32235)
                     /gene="csgF"
                     /locus_tag="ACHXOX_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR7005180.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(32260..32649)
                     /gene="csgE"
                     /locus_tag="ACHXOX_001693"
     CDS             complement(32260..32649)
                     /gene="csgE"
                     /locus_tag="ACHXOX_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR7005181.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(32654..>33163)
                     /gene="csgD"
                     /locus_tag="ACHXOX_001694"
     CDS             complement(32654..>33163)
                     /gene="csgD"
                     /locus_tag="ACHXOX_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR7005182.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         9873 a         6865 c         7355 g         9070 t
ORIGIN      
        1 tgaaactaat ttctaataag gccaaattaa aaaagattct caaaaatgca gccaaatata
       61 ttacgccacc gcctaaacta cttccatcgg aatggtgcga agcgaatatg gtgctggtag
      121 atggtcctca agctggggat aaagttaaat tgctgtcatt ccagaaaggc atgattgatg
      181 ctcctttcct tgagaacaag aaaaagtatg tcctgatgac cagtgcgcag atcggaaaga
      241 ctaccatctt gaacggcatc ctgtttaacc agatggctaa cgatccatgc aatatgatta
      301 tcggacaatc aaccgctaaa gaaatgtctc aatatctcgc tggtaagatc cgaccgtcta
      361 tcgaagcatg tgatgcatta aaagacgtgg ttacagacaa gaatgatcgc aatgcggtta
      421 acaacaataa ccagctacag ctaaaaacga atcacttcct ttacatggta tcgctaacca
      481 gtccatcgac cctacgtggt aagaccgcaa aggttggatt gcttgacgaa atcgatgctg
      541 ctacagcctc agaagaaggt gatccggtgg cactggctgc taaccgttta actacctttg
      601 gcgatgaagg ccgattagtc gtatccagta ccccgaccag taagctggga agcattaacc
      661 agcaatggtt atcaagcgat atgcgtatgt tttttgttcc gtgtcctcat tgcggtagac
      721 accaggtgat cgagtgggaa aacgttcaat ttgaatggcg taacatcgat ggtaagaact
      781 tgcccgatcc tgataccgct cgttatattt gcccccactg taagaacgca tggacagaag
      841 gggaacgaat cagggcagta gcgcaaggcg agtggagagc aacccgtgaa agtgaagtag
      901 ccggattctg gatcagccgt ctgtattcac ctttcagcac gatccgcgct tgtgtggttg
      961 attttagtca tgcgtggcaa tcctttgatt tacaatcatt ttataacacc gtgttaggca
     1021 aggtatacga cgatcaggac acggcagtag aagcaaatga actggaacaa ctcaaaacag
     1081 atgtttctat cgagaatatc cctgatgacg tgattttctt gtgctcagga attgaccagc
     1141 aattggaccg cgcagaatct accatcttag gtgtagcgaa agataaggtt tacattctgg
     1201 atcaccgcag cttttacgat cataactgtg aacgatatga atcacctgtt tgggatcgtc
     1261 tggttaactt caagaaaacc aaattctata acgttaatgg tgaacgtgta ccaatgctcg
     1321 ccagcttcct tgatacatcg aacggtcgat tcactcaagc cggataccgt atttgcggca
     1381 agtggaagaa cctacacgcg atcaaaggta gttcatctgg taatgctcca gtgattccgg
     1441 ttaaacctac ccgcacaggt ggtcatgaat tgcttatgtt gggtgttaac gttgggaaat
     1501 ccgctgttcg tgaaatgctg gttagaaacc tgaaagataa tcctcatatt ggcttagaaa
     1561 tatcagaaac cgttcctgat gattatctcg atcagctttt gagtgaatcc atcaagcgta
     1621 ctactactgg cgtgcgttgg gtgaaaaatc caggtagtac cagaaacgag gctctcgact
     1681 gtctggttta tagctatgct gcttctcgct acgttctttc aaaaatgtca tgggataaac
     1741 tcattgcaat gaaagacagc ttaaaccgtg ttgtcgaaga atccgtagaa gctcctaaat
     1801 cgcaatctga cgagcaaatc gaagaaacta agccaatcac acgaccgcaa cgtcaaaaca
     1861 tcgccagacg cccaaataga ggccgtagct gggtaacatc gttctaataa ctcgccgtcc
     1921 ttcgggcggc ctccccctaa atattgttaa tccaataaca attaaataag gggtaattat
     1981 gagtttagaa ctaattccct tagtaattcg taaaggcgaa aaaatcacgc tggcgaatga
     2041 agagggtgta acaattcagg taggaaatag taaaggtatc atttatcagg ttgatgatac
     2101 tccggctaat cacgagatta aaaccttaga ttttgccgag ggtaaatata ccatcgtaat
     2161 aactttggaa gaagaactgg tatcaatgca ggaattaact gtattgccag tattcaccaa
     2221 acaatccaaa aaagaccatc tgcgggaaac tatcgccacc atagagcaag ttattttcgc
     2281 ccgtttatct ggagacgaag ccgcattatc tcaaatgaca gtgaaaggga atactttcgc
     2341 ctatgagtca ttggctgttc tccagcaatt aaagactgat tatgaacgtc agttatcaaa
     2401 attaattcaa gccgaacgac gtaaacaggg aattagtccg attaaaaata tcaaattacg
     2461 tcttacgcga taaggggtaa atcatgttta atctttttcg acgcaaaaag gcggtagaaa
     2521 ctccagttaa aactaatcac cgccagcaac aaccaaaaat tttcatcgat aagcaagtag
     2581 aaaaattcca gaaagaccta tctaaacgta gtttaggtct ggttggtgat cgcattgatg
     2641 gatcgcttca acaagacacc attacaggaa ccttcaataa ggctctcaaa tcgaacggta
     2701 agcgccttta tgatcagggt cgtactctgg ccttaaacac ttccgtaggc agtcgctaca
     2761 cgcaatacat caccgatatg gtggtaggta ctggcctaga tccgaagccg tcagttgtta
     2821 aatcaaatgg caaacttgat agcgcactga ataagcagat cgagaatgct ttctggaagt
     2881 gggcgcagaa tgctaaacgc ttctctcgta acggtcgctt taacttccgt gaactgctgg
     2941 taatggctga acgtgagcgc gttatgggtg gtgagtgctt catagtttta accaaagaaa
     3001 acaatgagtt aagtgtttct atcctgtctg ctgataagtg cgactggacg ctcaaccgtg
     3061 aagtaagcaa agaacgcgct atctatcagg ggatcgagta tgacgtagaa acaatgcgcc
     3121 ctgttgcata ctggtttcgt aaaatcaacc tactgactca gacttacaca ggtgataact
     3181 atcgcgtaga tgcttcgcaa gtatgccatt attatcaacc acttgcggct gaatctctgc
     3241 gtggtgtgac tgacttcctg ccagtgatta aggatattgc gcatcaagac gcattccgcg
     3301 aaaccgcgat cgtccagaaa cgtattgcgg ctagttctat gggctttatc gaacgtccga
     3361 aagattctgg tgacgatttt gatactggtg aagatgatga acaatatcaa gcgccggaag
     3421 tagtacagga ttttgcacct ggtactattc aggaattgcc ggaaggggca acgattaaat
     3481 caatccaagc tactcaatca ggcgatgact ttaacagctt caatgatgcg atgttcacta
     3541 gcgtatcaat gggcttaggc gtgtttaaac agggcttaac tggtgatact agccaaatca
     3601 actacagcgc cgcacgtttc ggtgaactac ttcaacgtaa ccgtgttaaa tcgctacaaa
     3661 acaaattgat tgaaacagtg gtattgccaa tttttgaagc atatctacgc cattattccg
     3721 cgcgtggtat tgttccgatt cgtattactg caatcccgca tattatcgag aacactacta
     3781 ttattcgtcc gcgttttgag tccgtcgatg ttattaaaga tgtgaacgct gatattgctt
     3841 taattgataa aggacttaaa tcacgtactg ctgttatttt ggaacgtggc gacgatccag
     3901 aaaaggtatt ttcagagatc aggtctgaat ccagtgcact aaatattatc gttaatggtg
     3961 agggtgaaga aaaaaattcc ccagccgatc cctaataacc aacggggggc gcaatgcccc
     4021 caattaatta aaggtgatta aatgcttaaa tttcgccgcg atcttaacgg ttacggtggg
     4081 gttattaatg aagggcagaa tgatcaatac gaatttgaaa ttgctttctc cagtgaacag
     4141 ccttatcagc gccgattctg ggatgagcaa aatcaagaaa tggtggtatt agatgaaatt
     4201 ctggtacata caccggaagc ggttgatctg tctcgtctga ataataacgc tccgttgctg
     4261 ttcaatcata ttttcgataa tcacattggt gtcgtttgta acgctcgaat cgatgcggat
     4321 aacgtaggcc gtgctctggt taaattctcc aagcatggca ctttgtctaa tgatattcgt
     4381 aataaagtaa ttgaaggtac gatggaaaaa atttctgtcg gctatgacat taaagaatat
     4441 cgcatcgatt acgccaaagg acaattgatt gttactaagt gggaaccttt tgaaatttca
     4501 tttgtgacgg tccctgccga tgattctgtc ggtttaaatc gctctctaaa tactatcaca
     4561 gttaatttgg aggctaaacg cgatatgact aaagaacaaa tcgaagaaat caaagaagaa
     4621 caagaacccg ctcaggttga agaaactccg gtagaagaaa ataaagaacc ggaagttgaa
     4681 aaaactcaag agcgccaagt tgaagagaat aaagaagatg aaaatctcga agacggaaaa
     4741 gacgctaaac atcctgaaag tgttgatgat gatagttcaa ctgttcggga agaagaagta
     4801 aaagaagaac gtgaagccgc tccggttgaa gaagaaaaaa ccgaggaagt ggctgaacgt
     4861 tccgaagaag acgaattaga aattcgcgaa attgcacgcg agttaaatat taacgaagac
     4921 gatgaagaat ttaaacgagc attggcaaat aaagaaatta cgccggaagc attccgcact
     4981 aaggcactaa ataaaatcgc tactgctcaa cgtaacaacg aacaaattaa ggactctaaa
     5041 atggaaaaaa cttttgacct gaacaacgta attcgctctc tggtagatgg tgctgctctg
     5101 ggtgctaacg aagctgaata ttccgctatg gctgctggtg ctgcaatgca gcgtggtcgt
     5161 gctgctcgcg gtggctctgt attcgttccg gctgctgctc tgcgtgctgc ttccgaaggt
     5221 aacaccaaag ctactctgac cgctgttact gatgaaaaac tgctgaccga atcctacatc
     5281 gaaatgctga tgcctcagtc cgttctgggt cgtctgggtg tgactgttct gtctggtctg
     5341 aatagcccga tcgctgttcc gaaaatgacc gcttccagcg ttgacgcttt cggcttcgtt
     5401 gatgaaaacg gtgctgcacc ggaaagcaaa gctgaatttg caaacgtgaa aatggctccg
     5461 aaaacttttg ctggtggcaa cccgatcagc cgtcagagca ttaaaaccgt tccgggcatc
     5521 gctaccctga tcaccgatca cattaacaaa tctgttcgtc tcaaactgga acaactgatt
     5581 ctgtctgata aagaaaatgc tcgtggtccg gctggtctgg ttaagcaact ggtagacgcg
     5641 ggtcgcgtta ctaagaaagc cgctttctct tacaaagact tcctgaaaga aattgcagca
     5701 ctgaccgacg ctggcgttcc tgctcaggcg atcaagtttg cgatgagcgg tgcaactgct
     5761 gctgaactgg aatctaccct gaaagataac ggcgtttccg gttatatcat cgaaaacggc
     5821 aaactggctg gttacgaagt agttacttct ggtgttatcc cggcagacca catcgttctg
     5881 ggcgcgttcg acggtatcac cattggtgag tggggcggtc tggaactgga tatggacgac
     5941 actacctacc gcgcacagtc tgctatcgtt ccgcgaatct gggtagacct ggattatgtt
     6001 gttactcagc cggaagccct gaaagttctt cacatctccg ctgaatgaac tcctgtagaa
     6061 ccttctgaac cttcccccga tttgggggaa gaaaatctga ttccatctca ggaagaagaa
     6121 gttcaagagg ttacggcgaa agccacagct aaaaaggtgg gacgtccacc gaaagcaaaa
     6181 taataattag ccctgcctaa cggtggggct tttttgtatg taaatactcc ataaaggggg
     6241 taactatgtt caaattatca gaatcacaat tatcaagaat gttccgaagt gctcctgtat
     6301 tttcggtgga aggtggtaaa tcaattcgtg cttatcatga aattaccact accgacgaac
     6361 aaggggtaat tacagaatca gaatttctat tttgtcgtga gggagactta aatcagggtg
     6421 atgttgtcat tgtagacggt gaacgcttca aagttcaata cgttaagcgc aatggtgata
     6481 atacttctga ttgctttatt gctcgtgcag ggggtacaca tgctcgctac cgttaataat
     6541 atgcctagac tgaaaatcaa acgcgccttg caggatatta tcgaacaaga tttaggtctg
     6601 gctttaaacg tagaacaaac tcagcaaggc tttagtgatg acgtggtttg ttggattacc
     6661 ggaatgaatg agacttacac aagggtccgt ggtggtaatg caatgcaagc tgaatgcgtt
     6721 atcgaaatgc agttgtattc tcagaatcat gaaaccaaaa ttcatgaggg tatttgccag
     6781 ataatccaga ttcagccgga taacccacgt tttaaagatt tgggcttctc tatttcagat
     6841 atcactccag tagcctctaa taccgattat gacgatgatt ctagtgatgg gggtatcgtt
     6901 gggacactta gccttaaatt ttcttatcta gcgcgttttt aaggggtaat aatgaatatt
     6961 acacaagata acttagatat tttcactgga tcacatgttg aagtgtcgat ctctaaccag
     7021 atagacaatc aggtcgactt tttcgatcct agctttagtt ctatggagaa catcgcagca
     7081 ttccctacgc taaccgaatc cacagagata gaaactctgg aagagtacga tcaggacgct
     7141 acggggaaac ttgctggtta tcgtagactg gaaccgacaa cactcacatt aaaccgtgtt
     7201 cttgacgatg aacatcaagc aatgttgatg aaagcggtag aggataaaac acctttacgc
     7261 ttccgtatgt tctatgttgt gaactctggc tatagtgctg ctaacacagg gtacttttgt
     7321 atattcgatg cttacgtcac atcacataaa acccgtggta gtgataacaa agctgtaaca
     7381 ctggaattta aacttgaacc agatggcggg attttggcaa ggggtattgc taccgaaggc
     7441 cggatcttac gtcagggtga ttttggtttg ggtgctggtg tacatccatt cacgggtccg
     7501 attgatagcg aggcattagc cggaaaccgt ttcgtaactt acaagggaac tgctagcggt
     7561 aatccatatt caaccgacac agcattaatt caccttcaag ctaatgagca tggcgcatgg
     7621 caattaacct gtaatacttc tggcgcacca cgtttacgcg tccgaaatat tcaggaaaac
     7681 ggtcgctctg aatggatcaa ggtatattcc accaatgaga aaccgacacc tagcgaaatt
     7741 ggcgcggtag ctaaaactga tagagtcgat ttcggggagt attgattcac ttcctaaata
     7801 aaacatgacg cggggaggtt aacgcctccc ttattcctgt tttatagtga ggtgattcta
     7861 gatgcaatca atccaattta aacgcacaca gacggctggt aaaaagccga cgccggaaca
     7921 attatcacag ggtgagatcg ccttacagtt ggcggaccat gtgatttaca ccaaagacaa
     7981 aaataacaat gtcgttcaaa ttagtgtttc tccagaaaaa cacgctgaac tgaatacgaa
     8041 agtagataat aacaaaacta gcactgaccg tgttattgct tccaacaaac aagaagccgc
     8101 tgataatctg gctagtgcta aagccgaact gaatcagact atcacttcta cccgtgatac
     8161 cctcaatgct tcaatcactg ctggcgatac cgcagccaac aaacgaattg atgctttgac
     8221 taccacagta ggtaacaata aaaccgaagc tgctaacgct ctggcggctg ctaagactga
     8281 actgaatcag actattactt caacccgcga tactatcaac gcgacaatta acaagaacaa
     8341 gacagatatt aatgcgcgag ttgatcagac gaataccaac gttaccaact taacgaatac
     8401 tgtcgcagca aataaatcag caatagaaaa gacggtagcg gataataaga aagatgctga
     8461 ttctaagatc accaatctta ctagtacggt ctctgcgaat aagacagcca ttgaaagaac
     8521 tgttgcaaat aacaaggcca gtgccgatgc cgcaattgcc gcagctaacc agcgaattga
     8581 ttcgattgaa ggtagtgccg atgctgctta catcaagaag aataccaata ctaagcatgg
     8641 tgggtatctg ttaagcaaga cagctaacta tttggaagac cagacagcga gagatcttaa
     8701 ctactttggt gctttccgta ccaatggtca agatggactc atggatctaa ctcttaacgt
     8761 tcctcactct tccggtaaag cgcacggtcg cggatttact ttccgttatg cgtctggtgg
     8821 atctcgtgtt gaaacctatg gttttgatag agaaggacag aagaacttta gctataagat
     8881 gtatcacgaa ggtgataagc cgactcctgg cgagattggt gcatacacca aagccgaagt
     8941 tgataagatg tttgttaaaa acgtcgttat gtctgttccc aattctagcg aaaacgtatc
     9001 tgcatatttc aaattggcaa ccgcaacaat tccacaaaac gggcgtagtg tgtttttccg
     9061 tattcatggt ggtaatggtt acaacgttac ggcatatgat caggttgacg tagtagaaat
     9121 tcttctgcgt agtggtaacg atagacctaa aggtcttaac gtaattgcat accgtagaaa
     9181 tacaaacaaa gattttgagg tatttgctgt caatacttcc ggtgataact atgatatcta
     9241 cgtgaaatat cagcgttata ccgataacgt tattgtcgaa tatgggaaat ctgtttatgt
     9301 aagtttgacg gtttatgata cgccggaagc cacgttaatt aaaccttccg ttggtgttat
     9361 cggtggtcgc aacgtaactc tttttaatac tgaaaataaa cgtggtgtgt tgagttttga
     9421 cgacaataca caaaacagct atgacattgt tcatctaagc aatgatagag gtactggacg
     9481 aaaatatatt cgtaaattcc gtagcaacta taacgaaatg atctggcatg agactgttca
     9541 aggttccagt tatcgtctgg ctactggtag cactgatgct caggaaatta tgactattga
     9601 atctagtagc tcaattgctg gaactcataa aggtaatatt atttctggtc gtatgctgtt
     9661 aaatggtgga tctaatgcca ttacactacg ccgacctgct ggtcaatcta accacattgc
     9721 gtttcaagat aatcggactg gagatattac ccgtcaagga tggatcggtt atgccaatgc
     9781 tgatactaac gtttttgaat ggtatagtga tgtaggtggt acttctattc gtcaccacat
     9841 cgacggacag atcgaacttg caactggtag aacaaaacga gtttatacta acgctcaatt
     9901 catctcaatg aatagcgacg cctaccgcat ggtctacggt aattatggtg cattctggcg
     9961 taatgatggt ggtaaagttt atcttctttc tactgccgaa aatgataaat tgggcgggtg
    10021 gaatggaaac agaccgttca tttacgattt gactaacggt aaagttactt taggtggtga
    10081 tggtaacgaa ggtgcattag ttctcgaaag agacagccgt gctgcccgtt ttagcaacaa
    10141 tgtattctta gaaaaaggat tgcttacttt ctctgcgggt gggaatcagt cgatggattc
    10201 tttcactatt aaccattggg ggaatagtaa tgccaatcga tataacgttt tgcaattcca
    10261 ggacactagc ggaactcact ttaccactga acgtaacgga tctggcggtt tgcttgctca
    10321 tttccgaggg gatttaacta ccgaaggaaa attaacgtgg ggtaagggta cagctacttc
    10381 tagtttcaat attcgtgctt ggggtaatag tgattcccgt aaacaggttt tcgagtgtgt
    10441 agatgaaagt ggttggcatt ggtataccca gcgaccgggc ggtccaggta cttctactat
    10501 cgaatttgcc atcaatggta ctgttaagcc tcaagcaatc cacactggcg gtaatatcac
    10561 gcttaacggt gctgatattg agtttcgtcg cagtggtaat aagcatttat ggtttagaga
    10621 tccgaacggc ttagaattag gtttgatgta ctgcgatgat gctggtgtta ttcgcttccg
    10681 tggtcagaaa caagcccaga cgtggaaact tgcggataag atgattcatc ttgaatctgg
    10741 tagcgtcggc ggatctgata aaggtttgat tcgtggtaac gtttctggtg gtagctggtc
    10801 tagttggcgc gatcgtgcat ccggcttgca agttgattgc cctcaatcaa ccgattccgc
    10861 tcataacatc tggaaagcta ctcattgggg taaatatcac attgcagcaa tgggtgtaca
    10921 cgttcctagc ggtactatcg gtaacgctct tgttcgtctg catgttaacg atactaactt
    10981 tgactttaac gctgctggtg actttaccgc aggtcgtaac ggtagcttta acgatgttta
    11041 cattcgctct gactcccgtt tgaagattaa caaggaagaa ttacaggacg gtgcattaga
    11101 gaaagtaaac tcactgaaag tctacaccta cgataaagtt aaatctcttt ccgatgacac
    11161 ggtgattaaa cgcgaagtag gtattattgc tcaggatctg gaaaaagtat tgccggaagc
    11221 agtaggtatt caatccaccg aagatccaga aaatccagaa gcaatcaaga ctatttctaa
    11281 ctctgctgtc aatgctctga ttattaaagc tatgcaggaa atggacgcca aatataaagc
    11341 gcagatcgaa gcattacata aagagattgc cgaactgaaa gcaactaaat aataaaagtc
    11401 gggggactag ttcccccgtt ataaacaatt ttaattaagg ggtaattcta tgtctcagca
    11461 atttaaagat atttttactg gtggtctggt aagtatgttc tatcacgccg ataccactaa
    11521 caccgctctc gatcatgaaa gctacgaaga aattaaagag tgtgctggtt ttcctgaaac
    11581 tggtatcgag cgcggcactg tagaagttaa atccttctct tctcagtata accgtaagct
    11641 ggtaggtaaa ctgaacgttc ctgatctgac tctgaccgtt aactacatcc caggcgatgc
    11701 ggtacacgag aagctcatta aagctgccga agacggtact cgtattcaga ttaagattga
    11761 atactatgta gatgcagcaa aacagactgg tattcgcacc gctttcaacg gcttcatctc
    11821 taaagttgct atgaacggtg gcgatgaaga agtggtaacg aaagagttta cctttgctgt
    11881 tgatggtgct ccgctgaaac agcaaatctt cactgccgga tgaactaacg aagaacttcc
    11941 tttaccagaa ccggaaccac tgccggaaca tgaagcggtg gtgattccag aagtagaaga
    12001 agttacggcg aaagccacag caaaacgagg ccgcaaggct aaagattaat tataagccct
    12061 gccttaatgg tggggctttt ttattggagt aatcaaaatg gcaaatgtcg ttaaagttcc
    12121 tggctggatc gggtcatctg ctgtatcggt gacgggtaag cgatggatga aagaagcatt
    12181 aacagcttta aaagttccgg ctccgcgtaa tatgtcggca atggctggtc gtggtatgga
    12241 tacggttgta gctactgcat cgtggtctac ttcattgggt aataactggg gtgtaactgc
    12301 ttcaaactat cctgtttccg gtatgcaaag caaaggatct atggagaacc ccgaaaacgt
    12361 gggcgtaggg cgtctggttg gcgttatcgt tggtcaattc aatggcggta ctcctactat
    12421 ggctgtatat ctccagaacg gtagagcggg gaatataacc gttaatttgg gtggtgctgc
    12481 tgtcactgtt ccttataaca gtatgcaaag tggttttcat tactattggt taagcaatcc
    12541 tccggctgct ttcctaacaa atattaagaa gactggcact aagcagactt tgaaaatctc
    12601 ttaaattcta aataaataca gcgtaatcac tattaatgag gaaacaataa tgaatctgaa
    12661 tgaaatgctg aaagctcttt ctccgaaacg tgaatcttta accctcggcg gatttacgtt
    12721 ctatgctcgc cctatgtcgg tgcaagaatt taatgaacat gttttcaata ctgataaaaa
    12781 agaccgcgat gaacgctcta ttcttcgttg tattgaagat gaagacggta agccagtatt
    12841 tgaatctatt gaacaagtta aggcactgta tactagcgtc cgcagcgaat taatcggctt
    12901 agttgctcaa gcatcattga tgaaagatgc ggcggtaatt gaaaacgagg taaagtaaac
    12961 ccgctcttga atttttattt tcggcaaatg atgcgtcggg gcttgagtaa agatgagatg
    13021 gatactatgc caattacgct attttgggcg ttgcatatat tcgatacata tcttgaacca
    13081 caaagccccc tatatcaaga tgcgcgacat gcacagtcga tgtattacat gcacgttaca
    13141 tctcctaaca tgactcgtga atggttgaat aaaatcaatg ttaaccaatt ccgaatgatt
    13201 aaggacgaca agcaatttaa aacgcaagaa gaaataaaag aaattgctcg taagaaagag
    13261 gaagaacgta actctgcttt ggttgatagt tatttcgacg cttcattatt acaaaagctc
    13321 aagagcggtc aattggggta atttatgaca agacatatag taacaataga agggaataac
    13381 gacggtctaa ggagaagtac caaccaagcg attgacatgc ttgataacct ttcagaaagg
    13441 gcaatgaatc ttgatttgtc tggtggttta tctagtttaa gtggaaccct tcgcggaact
    13501 ggtggatcgc ttgctttggc tgctggtggt tttgttggtc tggctgctgg tgctgtctct
    13561 gctgcaaatg ccatcggtga ttatgtaaag gaatacagcg aagtttcaaa agcaactggt
    13621 ttaaccgtcg aaacactcca gaaattagag aagcaattct ccggtactgg cttaaccgtt
    13681 gagaaatttg gtgatattaa caaagacacc ttagataaac tggctgatgc gtggcgtaat
    13741 ggtggcggta ttgaagatga cctagaaagt gtaggtttga agattaaaga ctatgccgag
    13801 tttatgaacg atccacaagg cgggatgaaa gcggcgatca aggtcttcta tgatatgcgc
    13861 aaggctggtg cgtctatcgc tgatgttaag tttatgatgg aatcgttagc cagtgattca
    13921 agtcatatgg ctgatgaatt gcagaaatac ggatcttatc aggaagccgt aaacgccatt
    13981 aatcagcaaa ctgttaaaat caccaatgat acagccgaag aatataataa attctctaaa
    14041 aatatggata cccttaccga taacttaaag ggtatggctg caaatgcgct aaatccagtt
    14101 gttgaaggga tgaacaagtt atacaactgg tttgataaag attgggaaaa gaccgcttta
    14161 ttcaacgcat taaagacttt taatgaaagg tttgaaaaag ctcgccagaa tatggctatg
    14221 gctaacggtg gttttattaa caagcatggt tatagttcta ctggtctagc aggtggacaa
    14281 gccagcttca acaataaacc aaattatcct gatcgttggg tggatagtga aggcgttacc
    14341 cgtgataaaa acggtgatcc ggtaattact atcactggaa ataaatcgga taccggattt
    14401 accccaacgg gtcaaggtgg aggcgtcacc ggaccatcga aaaaagaaca agaagaagca
    14461 gaaaaagcgg cagaagaagc aaggaaggcc gcagaaaaag cacagaaaca gcgtgaagat
    14521 gcaattaaaa agctgaatgc tcttgatgtt agattgcacg gtcagacagc cgcatctatt
    14581 gcttctcaaa acaagcagtt agaagccagc ttgaaagatt tagacactgc tttagatatg
    14641 ggcttaatct ctcagcaaga cgcagccgcg aaacgtcagc aactgttaga ccagaacgca
    14701 gaaaacatct ataaaatgat gctgggtgct gatccggttg atgctttgaa tgccttaaca
    14761 gttttgcaac aaatcaggga caacgagtta gaaagccata aacggttact tgatggtaaa
    14821 gctatctcct atgaagaata tatgcgtcgc gtgaatgata ccgaacaaaa ctattctcag
    14881 attgatagtc atttacaggg aatggatggt tttaaatcca atcaattaac cagtggattt
    14941 gattatcaag actcaaataa tccgtttgct aaatttaatg caatcgataa agagaaatcg
    15001 gacgctgaac aagattataa aaccgataaa ctcaagattg atggtattaa tgatccggct
    15061 aaacggatgg ctgcattaga aaaactcaat gaaaaccatc aaaaacggat ggctgcaatt
    15121 gaaaagaaat atgctgatgc tcgacagtca atagccgatg atatgtatgg cggttttgct
    15181 gctgcaatga ctctcttcgg gcaggaaaac actaaagcaa tgcagatggc tttcaatgct
    15241 cataaagcat tctccattgg acaagcgacg gtgaacatgt ggacggctgc taccgatgca
    15301 tggaacgatc cgaccaacgt aaccacaggt caaaaaatcg cggctgctgc attggctgtt
    15361 tctcagaaca tggggaacat tgcaaacatc aagtctacta atgttagcgg tatggctcat
    15421 gatggtatcg ataatatccc tcgtgagggt acatggttgc ttgataaggg tgaacgagta
    15481 gttgatcagc gtactaacgg tgatttgaaa gacttcttat ctgctcaaaa atcaggtggt
    15541 ggcaactctc aaccgattga agttaatgcg cctttgaaca ttaacggcaa cgttaatagc
    15601 tcagataaga tggtcatgga tgctatcaaa cgtcatgcta agttagttgc tcaggcggta
    15661 gaagacgcac agcgccgtaa gatgtaatta aaagccctca tagtgataaa taatcataaa
    15721 actatggggg cttttctatg ttcaaatcaa agaatattaa aatcacagat tttactctta
    15781 aatcaaaaca gcctttcttc aaggcgcaat ctatttctgg taagttccag cgtcgcttta
    15841 ctggcattca tttttatgaa gcagaattta cagctaattt catggctcag gatattaacg
    15901 aagtaaaaga atttgtagca cgtcaccttt ttggtcgtcc tttcagtgtg ccgctgtctt
    15961 acttttcaaa atatacaggt gatgtacgcc agatggtaac ggctgctgct ggtactgctc
    16021 gcggtgggcg taaggtgaga atctccaact tcaccggaac actgaaagcg ggaactgtca
    16081 tccagtttga gaaccacaag aaaatctaca cgatcaccga agatgtgaaa tcaggtggtg
    16141 aaatgaaact cttccctaac ttgcgtcaga acgtcctggc gggtgaggtg atcaagtatc
    16201 agaacgtaga aggtgaattt gttctcaaaa ctgaaaatat cgattggaag atcgcccaga
    16261 ttggcaagat gaaattcgaa ttagtggaga atgtataatg gcaactattc aggaatcatt
    16321 cagcaaacta tgcactaatc tggacttcat cgaggtctac aacgaccaga caggtcagaa
    16381 cgtgtctaga ttgacgctac cgcagctttt ttccactgga tcgatgtttc acattatcga
    16441 agtgataaca gcgtcagggg acgttctacg gcttacagat gggtatttcg atttggacta
    16501 taacggattt acgtatctcg caacgggtga ttttcttcaa atctcatcga ataccgaaga
    16561 gaaggagatc aacaacaacg ggatcaacgt aaccgtttct aacgttcgcg aagaatacat
    16621 taccctgatt cgtaacaagc aattcgataa atctgatgta aaaatcgaga tggttttcct
    16681 taaccctaac acgggcaagg ttgaaactac ttaccctgtt ttccgtgggg tagtcgattc
    16741 catcgggatt aacatcgaac atgaagatga tgagtgtaaa aacgaatcag aatttcagct
    16801 taatagtatc tgggaagttc tagataaaaa cgctcgaagt catgcctccg atggtatcca
    16861 ccgatcctat gttggaaacg agaacgatct attcttctcc cgtgccggga agtggcaatc
    16921 agaatcaaaa tggtttagct ctaagaaata atcccttctc cctaagccta gtaaataact
    16981 gcatggaggt attcaacatg ctaaaaacta ggcttatcac cgattacatc aattctttaa
    17041 taggtcagga gttcgttcag ggtgagaatg attgcaatat aattgcatgt aagatcatcg
    17101 atattctcgc tggcactgac ctatataatt ctctttataa aaaatattca acgaaagaag
    17161 aaggcttgaa aatctgcaaa gaattaagcg ggtattcaaa catccttcaa ccaattaaga
    17221 aacatttcaa attagtcact gatgatttac aggacggcga cttactggtc acagcccaca
    17281 aattaggaaa ccgtaattat tattccgtag ttcctcatta ttccggttat ggcctcgttg
    17341 aagaagatgg tatctggatg accattcctg tttcagacat tgactatgaa caagtttata
    17401 gattcggggg tgaataatgg gatttgaaat attggtaggc gcggttattg ctggtgcgtc
    17461 tgctgggatg gctgctgctg caacattttc tgttatgacc gcagtcgcta tcggtatggc
    17521 tgctggtgca atgaccttga tcgcttccac tgtaggcgca ccaaaaacac cgaaagtaca
    17581 aagcccagat aatgcggtga cactaggaac atcaaacgat ccgaagacag tattacccgt
    17641 tcttttcggt actacccgca cgggtgcaat ctgcgtttac aaagcaattt ctaagcaaga
    17701 aaacaataag ttagtgcaaa tcttcgcaat tgcagagggt gaaatcgacc attacaaagc
    17761 actattcatc gataacaaaa atgttcttgt tggtaagaac atgacgatcc gtgatggtgt
    17821 actggacaaa ggcaacatta aagaagagta ccggaaagtc ttagaagttg agttccgcac
    17881 gggtaagaat cctaacaccg ctttgtcact ggcaaaacgt cacttagggt cagactggaa
    17941 cgacaactac aaaggcaatg gtattgcaac catgtgtatt gtgttacgtc gtgatgacaa
    18001 atctcttgct gctggtgttg atattctcca gcctaatagc caggtagcgg tcgatgttat
    18061 gggtttaaaa atccgtaacc ttgaaaccaa tgctattgag gctagcacta acggcgtgga
    18121 ccagattttc cactacctaa caaatgaaaa atatggttta tcagtaccaa ttgaaaacat
    18181 taacgttgat tcattcctga aagtacgtaa acaagtacgc cagatggatt tgcattcaaa
    18241 cggtgcatgt gatccgaacg ccagctttaa agagaacttg actagcctta tgcagacttt
    18301 cggcggggtg atgttcgaat ccttcggaag aattaccctg aaactggatg ctcctgatat
    18361 tgttaagcat accttcaatg aagacaatat catgatgggt aaggtatcac tgaaaacagg
    18421 tggcactaac ggttatttca ataccattaa cgcaatgtat caagaaccat caattgacta
    18481 ttcagagcaa atgctacgtt atccggctga tgctgaaaac gatgctacta ttcgtcaaga
    18541 tggtcgaatt atcgctaaag atattgaata tcgttttgtt aagtctaaag cccagattga
    18601 taaactagcg agtgtagaac gaaataaatc tcgtatcact caggttatca gctttatgac
    18661 tactgacgca ttcactgccg aagtttggga cgttattagc gtaacctatg atgaattaaa
    18721 actgaataat tcactctggc gtattactgc aattgatcgt tctattgatt ctggtattgc
    18781 tggtatgatg actatcaccg ccacagaata taattctcag gtttatactg acctgaacta
    18841 tgcggcaact ccagacaata gaccaagtgg tttaccggat tcaatgacag tacagaagcc
    18901 tactaatttc agaattaagg caactggcga gacgatttac ggtaaaaacg ttactttgac
    18961 atgggatgca ccggaagatt ttaaccgcta cggcttccag attgattacc gtgtaagcgg
    19021 atcacctaac tggattaagt tgggacagac ttcacagcaa attttcagtg tcaatgcact
    19081 ggcaaaagat cgttcttatg attaccgagt ttgtgctttc ggtatcatcg cgcgatccga
    19141 ttgggtggaa ctggttaacc agaatcctac tgttacctat gaattgccga ctccggttat
    19201 tcgaatcaaa aatcagggta gcacgcctgg tactttcgaa ggtaatgatc tgattatcga
    19261 atgggaaaat cagcaacaat tagatgtgat gatcaacggt gaagctaaca agtttagtga
    19321 cctgtttgaa gcatacatta tcaaggtgac taacaaggct ggtaagtcta ttcagtaccg
    19381 tacccgcgat cctgaatcat ggacttatac gcttgatatg aaccagttta acggcctttc
    19441 gcgtcaactg acggtagaag tatcagctaa gggctataac aactcagaga gcgccccagc
    19501 gcgtttagtg gctatcaacc cacagcataa gccgatgaaa ggttttagtg cgcgtggtgg
    19561 ctttaatact gcgtttgtta gctgggcgga tgacgtagaa catgactatg cagggtcaat
    19621 catccagtat gcaaccgata acactttctc agatgcaagg gcagtaagca cgaatagtgt
    19681 tagccatact tcctttgata ttgctgacgg tgattattat atccgtggtg ctcactacga
    19741 tattttcggt atggatgatg ctgtttggtc tgaaccttat ttcatgtcta tgaagtctac
    19801 cattagctgg gacgatcagg acaaagaagc cctggaagac ctgatcggtt tacaagatcg
    19861 cttagatgaa accatcgctg atgctattgc tcaagctggc gctaatgccg atgctaaaat
    19921 tgatgcaatg cataagcaaa tcactaccga gacgggtaaa actgttcaag cctcagccga
    19981 taccttaaag agtcttattg tctctggtga tcaagcaagt tccactaaga ttgatcaggt
    20041 taaagctgaa ctgaaaggcg atatcaccaa agaagttagc gcatctgcta ctaccctgaa
    20101 acaagcaatt gcaaccagtg aggcagcaag cgcaagtaag attgaccaag ttcgggtaga
    20161 aatggatggc aagattgctg gtgtgaatca ggaagcggac gttaagatcg atgctttgaa
    20221 gggaaccatt aacagcaaat acaatctagc agttaatgca gatggtcggg tggctggtat
    20281 tcacatgagc gcaactaacg atccggcaca accgactaaa atcatcttta cggctgataa
    20341 aattgctgta gctccgcaga acgggaccga tgtttgcccg tttggtatcg aaggtaacaa
    20401 ggtttatctc gataatgcga tgattcgtaa tgctgcaatt ggcacagccc agattaatga
    20461 tgcggcgatt acaaccgcca agatcggcaa tgctgctatc aactcagcga agattcagga
    20521 cggggcgatc accaatgcca agatcgtaaa cggtgcaatt gataacgcca aaattggtaa
    20581 ctatatccag tcttccaact ggaacggatc aaccggatgg catatcaaca agaacgggtc
    20641 cgctacgttc atgaacgcta ccgttaaagg taacatcacg gcggattccg gtactctgaa
    20701 caacgtcacg attaactcta actgtgttat taagggtatg ctcgatgcta ctcaggttag
    20761 gggtgatttt gttaaggtga ttggtcgtaa attccctgtt cgtgatgcca acgttgataa
    20821 tggttatgct ggttatccac agggtacggt cactgttcgt attgaagatg atcacaagtt
    20881 cgaccgacaa atcgttattc cggcaattag ttttggtgga ttgcgagcgc gtgaaggatc
    20941 gaacaataat accttttacg acacctgtcg cctgattgtt cgtaagaatg ggtcagagtt
    21001 gtataatagg gcgtatggcg gtaatactgg tctttactca ggtgttattg atatgccagc
    21061 gggtaagggt gctgtaactc taacgtttga agtacactca agcgcaatca acaactggac
    21121 gcctagcaca tggatcagtg atttaacagt aatggtaact aaaaaagcaa ctaccggtat
    21181 tactgtttct taaaaattta attttaataa ttaatcccgc ttaattcttt agagttaggc
    21241 gggattattt ttatctaaat aattttgcca ttaaactata taaaaatatc gggggcgtaa
    21301 tgactgaaat tatatatggc ggtatcggtg ttatcgcctt aatttgtggt ggtctttgga
    21361 gacttcaccg aaatcaatta gccacagaaa atagattgtc gaaattagaa tccaatgatg
    21421 cattgtttaa tcagaagttt gaaacaatgc agaataacca cgatcagata tccgagagag
    21481 tttatcgcat ggaacaaacc cttcacggta tagaaaagaa agtggttgcg atggacgcta
    21541 aatttgacca agttatcgat ttactcagac aaaaataata ataaaagggg tgaatatatg
    21601 aagaataaac ttaagaaata ttttggttat ctcttgattg tcgccctcac ttataacgta
    21661 gcaattagac ctctgcttac gtcctttggg cttgaactcc cggctatgac cgtggatgaa
    21721 caattgctaa ggacactggc gggggtcttt tcgttattag gggggtaaca tgtcatccag
    21781 tgccaataaa cgaaacgctc taaggactaa gaaagcgtta cgccaatgga ctgataaagc
    21841 aaccgataca ttcgaaaagg cgatagggga aggggcgatc tttgctgctc gtgcactcca
    21901 gaagaagatt aacaagaatg ttgatagacc tactcgctgg actcagcaag ctgtaggtaa
    21961 taccaactac aagaaccgat cagggaccag acaccaaatc ttcatcaagg gcgcaaggga
    22021 taaggacaag aagatcggca gtcaggatga ctatctgaaa cactatttcg atggtggcaa
    22081 aatcaataag ctagtgccaa tcgccaacgg taaggtctta gacgcccacg ggaacattaa
    22141 ggccatcaaa ggcggtaaga tgatgcgtaa catcgaaaat ggcaacttca tcaaggtaga
    22201 gaaccaggaa gggactttta tcatgaagaa gtacaagcct aagaaatccc gaactaaacg
    22261 cgctaagaat gggtcggcgg tggcaaaacg tcgcttagag aaacgcacac agaaacagag
    22321 taagcgaatt gttgccgtta agtcggacaa aatttctact cgttattcga caatgggatc
    22381 atgggaaagc aacgaggaaa tgatgcttaa aaacatcaat aagcacatta aatcgcgcat
    22441 gagatacgtt taatccataa ataccctcat agaatcttat gggggtataa catggctaaa
    22501 aatattttta ctgaatttcc tacttatccg gtcgatcagc tttccggtat ttttattaat
    22561 ggcattagcc cagaatctat gacacatgat tttgaggcga agagagttaa acataaacaa
    22621 tataaagaat gtatccgtga ccatgaaaag gggaccgtgt tttgtgtcgc tacattggct
    22681 aaacgtccta aatatcgttt tcgtgtaggg caagaagtcg atgtagttaa tccttatagc
    22741 tttaattgtc tgggcgatgc acgcgcggtg tgtgtaggca ctgctcctta ttatatcaag
    22801 ggtatgcgct ttattggtta tatcttcgaa atgatctaag ggggtaatat gttaagcaaa
    22861 catttttctc gcaaggaatt taaatgtaaa tgcggaaaat gtgattatga tacaatcgac
    22921 gctgaattac tggtaattct tgaggatgta cgagagcact tcggtaaacc agtgattatt
    22981 aacagtggca accgttgccc gactcataac aagaatgtag gcggggcagc caatagctat
    23041 cacgttcgag gccgcgcggc tgacattgtt atcaagggtg tctcacctga tattgttcat
    23101 gcttatcttg atgggaaata tcctactcaa tacggcttag gaaaatataa aacatttacg
    23161 catatcgatt ccagatcaaa aaaatcacgg tggaatggat aaaacaaaag cgcctctggt
    23221 gggcgctttt ttgttatata gcaattgttt tagttgcata gctgaacttg tcggttagat
    23281 aatagcggat tctctcaagt gcatgtttta ccgcataatt tttatgcttg acttttccgt
    23341 tacgaccgaa tatacacaga tgatctataa gatcccaaac ggtagcaata tccttagaac
    23401 catgtttacg caatgcacga ccgatactct gacgaacgat agttgattcc ttcactggat
    23461 gaccgaaaat aacatggtgc aaattcttaa ttgaaacacc agtagagaac acaccataag
    23521 acgcaacaca aatgatcccg gtgtcaccct ccgccatttt cttaaattca tcacgttctt
    23581 ctgtcttaac tccaccgtca atataataaa ccttgtcgtg taccttctgt aacgcgtcat
    23641 acataagttt tccgtgctta gtattgcgga acatcaggaa tacgttttcg cccttcttag
    23701 cgagttttag cgccaaattg caagcaaact tattccgtcg tgggttagat gtaatatact
    23761 tgatctcttc cgcatattct ctccccttaa ccgcgctaca ttcctcatcg gtataacgaa
    23821 gaaaaagaca gttgattttc agttttgtca cttgaccttc ttccatcagg cggtcaatac
    23881 tcacgatctt agaaatatca ccgaaaagcc cgacatactg cattaaatgg cactttgatt
    23941 ctttcgggga accagtcata ccgattttga actggcaatg attcattccg ttaatgatat
    24001 tggtaatatt tttggcacta gccttatgcg attcatcaac tatcaacatt ccatattgtc
    24061 ggaaccattc aggaggcatc ttgcaagctg attgccaggt actaaccgtg attaacctgt
    24121 ctcccggatg tttcccgcta ccactcatca ttgtatgaat tgcttcataa gggaatagcc
    24181 tataatcgat gaaatcatcc ctcagttgaa ccactagcga tgtagtcgga acaatgatta
    24241 gaacttttcc ggtgtaatgc tctaagtacc aacgagacag catacaagca atcaatgact
    24301 tacccgcact ggtaggaaga accagcattc tacggcgatt atggataccc tgaaagactg
    24361 cctccctttg ataccaataa ggattgattt tattgctacc ggaatacact tctaaggaat
    24421 caatccattc attaatcgcg tctttggtta catcttcctt atcaagcaat cgcgggtcaa
    24481 tccagactga ataccccata tttttaacaa agacacccaa cgtttttaat agtccaattg
    24541 gtagggtgtt ttcatgcgta aacagtctta ttcgtccatc ccagccagaa tacttgtaac
    24601 gggggctaaa cctagcccct tcaacctcaa acgagaaata atctcttaat tccattccga
    24661 tagaaggaga gcaatcaacc ttaacgaaac tgtaatcttg aaaatgaatt ttaatatctt
    24721 gcattagttc cactccacac ttgcaatatt ctttatattg tttagtgatt tattgcagta
    24781 gtggaatgtt tcagttttgc cgtattcaat cccgcctata cccacatcca gtaatctctt
    24841 tattgctctt tcagtggcta aagcatgttt cattgatggc atttcaccag caaagacaat
    24901 atcaaagtta tgaccgggat tgcgtacacc cagattaaca cgtcgcttga tgattgactt
    24961 tgtaacacca aatttaccaa tatcccctga tttcattaca tagagataag gcacacccgc
    25021 tttatattga aagtaatcac ttttgagaca agcacagcgt ttaccttcat taagcaggtt
    25081 ggtagctgat ttgtcggctc tattgccgca tcgggtacat gtaagagcaa ttcttgaatg
    25141 aagcccttta aactcaccca gaaagccatg aaagactaac ggtaagtcct tctctttgat
    25201 gtgagtcaga atgcgttgtt ctaatgccga tggagttaca gcacgggagg atttaggagt
    25261 aaaaacaata tctttagcca ttactggaat catatagacc tcactttttt aacttatagg
    25321 cataacctca atatggttgg attgggattt agggtcgcgg attatacaca tccagataga
    25381 gaatgcaata gtaagacaat tttctctgat aaatagtagt gaacttatta ttttagagga
    25441 aactactatg aaacttgctt acgctgccga aattcctttt gttaagaaaa ctcgcggttt
    25501 atcgccggaa gagtatcaac aacgaatcat cgctaaactg gaatctgctt tcgtatttgg
    25561 tggatttatc ctaccgtgga agggtaatca cacttacttt cgaatctaca atttagatac
    25621 acaagaatat aaggactaca aattacgcaa actggaagat actaacgggg gtgagttatt
    25681 ccagactgaa aaggctgtat ggttgaaaat ggaatctcgt tgtaatgaga agggaaagaa
    25741 attcctcgga tggcaaggag aatggaaagg gcgaaataga acgaaagccc gtgttttgtg
    25801 ccctgaacac aatcaaatca tgacgccatc tctattacat gctctcaaag atgatttcga
    25861 ttttgattgc aaaatctgta tggctgaaaa atcccaacgg gtaagaagtg gcaagacttt
    25921 tgatgaagtt attaaggaca aagagacaat catcaatgca cgttgcgaga gtacgcctta
    25981 caccttcaaa ggattcatca taaacacccc acacctaaag gatgttaaat ttaaaaccta
    26041 ttgcaagaca cataaccacg aatgggaatc tcatttaaga tgtgctgatt ctttcacttg
    26101 tccgctttgc attaaagacc aactggtgca actgtctaac cggacttatc agggtaaggc
    26161 tagtttctac attcaattat tagatgacaa atttattaaa tttggaatca ctacccggaa
    26221 accagaagaa cgaatgagag agcaaaccag aaagagcaac ttcacccacc gcttaatctt
    26281 tactcatgag tttgatgatg gttggaaagc cgttgattta gagcatgaag taaaacaacg
    26341 cttcaagact catgcagcac cctacaaaga tttcaaggat ggatggtcgg aaactctgac
    26401 tattgatgac ttaccacact tacagcaact ggtttatgac tacctaacca atcaaccgga
    26461 cgaggctaat atgtgggtat ctccgaaaga tgtatttgat gacgatacat tcaagctaca
    26521 tactcacttc tacggcatca ataaaccgga atttttctgt attgatgacg acagtcctga
    26581 tttaatggat gaatacttta atactctctt agacgctgcc taacgcaatt aaattaaaaa
    26641 ggggtacatc tgtacccctt aactgtttta gctcgttata cgtcgattta ggccgcttaa
    26701 attccaaatt cctgtaatgg acctggtatt tcctcttctg gtttccagtt cttatctgtc
    26761 aacatgtcta aacatggatg cagaacaagc ccgttactct ccagaatctg cctatcattg
    26821 atagttttca aatcaacgaa gatttgtcct ttcctcgttc ctcttgaatt ttctattaca
    26881 actctctggg tctttccatc gatgtattcc agtacaaaca agtttttgtt tctgtcaatc
    26941 ctgctacacc cgatccgctt tagagcttgc ttaatcttaa accggacctt aatgcgatcc
    27001 tcgttataaa catcttctaa atcaaattct gcgagcttct cccagccttg agagtcaaca
    27061 gaatacattt cctcttctgc catcttgatc tgattgtaga gtgtttcacg ctcgctattg
    27121 agggtagtaa tctgatcggc tagctccttc gttgctcctg tcatagcaga aagggcaatc
    27181 aggttatcaa tcttgcgtga tatctcatca acctgtactt ttaaagccgg aacaggatta
    27241 gccttatcct cagcaatcca gattttatca gccagcaatt gcaatacagc tttctctaat
    27301 tgatcacctc gaaaactcca gtttggatgc tcgcaatcaa gtctacgtga acgggtcgca
    27361 tcacatgtat aacgatactg gtcagaacgt ttgtttgttc ccttcacttt gaccatagcg
    27421 caaccgcaat gatcacattt caataggcca ataccactaa gcagggggat cggtttagct
    27481 tcttccttgt ctccaaagtt ccatgctctg gcaccaatat ttttcttaag gtgatagaac
    27541 gtagggtcat caataacacg agggtaataa tcctttaact cataattaac gccatctaca
    27601 gagacctcct tgataccaat caaagcgcgg gtatggaaca acctttcaac cattgcccgt
    27661 gaccattttg aatgcttctt attactaacc gctggtggtg gtgcatatgt ctcgttaagg
    27721 tgatccagta tctcggcggt tgaacgtcca ttcttccgta attccacaat ctcctgaaca
    27781 acagggaagt acaccggatg aggcaacaca taaccggaag tggtatcagt ccaccacata
    27841 ttctttccaa cttcctcgat tgcaactgcc ggattctggg gattttcttg atgagcttta
    27901 atcttaatca gtgcgcttga gttagtacga ttgcgcttag tctgactttc ctcattacca
    27961 cggatgaata gaaggataga gaacatcaga tccatcggat tagcggtaac agtatccagc
    28021 gagtagatct tgttatccat gccagtaaca atggttattc ccctacggat tattgaaagg
    28081 aataactcct gcgcactgac aatatcggca cgggataaac ggtccaagtt ttcaatgaat
    28141 agccagctac caacaggaac ggatctacct attgcatcaa ggaaacgaga tagcgcccct
    28201 gttttggagt tagcaccctt aaacgcagat acacccagat cctgatagtc atttaccagt
    28261 tctaagtcat actttgccgc aatctctctc gccatcctga tttgtcgttc ataactagaa
    28321 ccatcacttt gacgcataga cgagaagcga atatacgaat atagtttagt tttcatacca
    28381 ttctcaaaca aaaaagcccc gaccgaagtc gaggcgatat tacacacatt cagtctttga
    28441 agtcgatggt cacgttacct ttaagggcaa gttccattaa gcaactgcgc tcatccttca
    28501 tgctcttaca caatttcatc ccgtagacaa cgagattatc tttctctttc caatattttt
    28561 gtttatctgc ataaacctcg ctatattcac tatctttgag tgagagagca ttttctaact
    28621 ggctaatctc ctgattcaga tccttaatct cggccttagt tgaccggata gtctcttgat
    28681 gttgagtatc ggaatagtca tacccacaaa caaatccacc gatgccacca acacacacag
    28741 aaacaataat tgcgtagata gtctttttca taattgaatc ctctttgaat gaattacgat
    28801 atatttatat cgttaaaagt tgatgctaat aatcagacca aatgccacag cgcagattaa
    28861 atcagtccag ctaatgctaa gtgaataatc tttcttgaat gtcttagatg aaatgccatc
    28921 atgaaattgt ttcattgctc accccatact aacgacaaga taagaaagac taatcatagt
    28981 tgtgagacta cctcccacga atgagataaa agctaacatg tttgattctc cagtttaaaa
    29041 agcggtggga attgcaccca ccaaattaga aatcacttct tgctaataaa ctctttatgt
    29101 ataaaatcta agacttcctt taacttctta tcggtgccaa tcatcgccgg aagttcttct
    29161 ttaatatcgc gaggaagata cacattacct ttccagaaca agcaaccaca ttgagcaatc
    29221 gaatagctga acatccattc acaatcttgt tctatctccc agaatctaac ctgctcatct
    29281 tcgagttgaa gttcgattcc acggcggcga gcatagttat ggattgcttt ggtaatgttc
    29341 atcgttgtat cctctcgtgt gttgatgggt acatgatatt aggcaatccc aaatatgaaa
    29401 tagatgtgaa agccgttgct gaagcagccg cgaaacatca ggtggcgctg gaaatcaata
    29461 attcttcatt tttacactca cgtaagggca gtgaagacaa ctgtcgtgcg gtagccgcag
    29521 cggtacgtga tgctggaggt tgggtggcat taggctcgga ttctcacacc gcgtttacca
    29581 tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt agattttccg ccagagcgca
    29641 ttttgaatgt ttctccgcgc cgcttactga acttccttga atctcgcggt atggcaccga
    29701 ttgcggaatt tgcagacctt taattactag taatggaaat atattaatga acgagttttc
    29761 tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa ccgcaagatc ctttactggt
    29821 gccgctgttt accctgattc gtgaggggaa actggctgcg aactggccac tggagcagga
    29881 tgagttactg acgcgtttac agaaaagctg tgatatggcg caagtctctg ccgattacaa
    29941 tgcgttgttt atcggcgatg aatgtgcagt gccgccatat cgtagcgcat gggttgagga
    30001 cgtaacggaa gcggaagtgc gcgcttttct ttccgaacga gggatgccac tggcggatac
    30061 gccagccgat cacatcggca cattgctgct tgcggcttcc tggctggaag atcagtcaac
    30121 ggaagatgag agcgaagcac tggaaacact gttcagtgag tatctgttac cctggtgtgg
    30181 tgcgttcctc ggcaaagtgg aggcccatgc aaccacgcct ttctggcgca ccatggcacc
    30241 gctaacccgt gatgccatta gtgcaatgtg ggacgagctg gaagaagatt ctgaagagta
    30301 aatgtgatca tcgtcactca taactgcaac agataatttg ttgttgcata aaatgtgtgc
    30361 tcgatctcat tcatggccgc gttttctgct atcatgcgcg gcatgaacat acttctctct
    30421 attgcaatca caacaggcat tctctccggt atctggggat gggtggctgt ttccctgggc
    30481 ttactaagct gggcgggctt cttgggctgt acggcctact ttgcctgccc gcaaggtggg
    30541 ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg gcgtggtgtg ggcgatggtc
    30601 attatttacg gtagtgcact ggcaccacat ctggaaattc tcggttatgt cataaccggt
    30661 attgtggcgt ttctgatgtg tattcaggcc aaacagctgc tgctttcatt tgttccgggg
    30721 acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg actggaaact ggtgttacct
    30781 tcgctggcgc ttgggctggt atttggttac gcaatgaaaa acagcggcct gtggctggcg
    30841 gcgcgtagtg caaagacggc tcaccgtgag caggaaatca aaaataaagc gtgaggggca
    30901 ctcacgcttt cgcttaaaca gtaaaatgcc ggattattaa ttccggcatt tttatctgtc
    30961 aggattctgg tggaaccgac atatggcggt atttaaccag aatgtcattc tgccgttctg
    31021 ctttgttctg caaatcccac agaccacggt cgataccatc attaatcagg aaaatgaccc
    31081 ctgtttcgat agccgacatc agacacagca taacgggttc gtttgaggtg taacccactt
    31141 ccccttccag caagcgctgg tagtcaataa agcggaaaac cccggcctga acttcatagg
    31201 aaagtatcgt cttactggtg ttcaccgagg agaggatctc accggtactc acattgacga
    31261 cacgcaggtt cacggcaatc tgatcgagct ggtattgcgt gtctgcaccg atcccaaaat
    31321 atcttgcccc aacaccgccc gatttaacgt tgctttcata accgataatc gaaccttcaa
    31381 ccatgatatt tgccgccgtt aaagattgca gcgggattcg gttattaatg gcaactgtgc
    31441 cgttttcttg tgccgcacga ataatcttgc gctcgttaag caggttttgt aaactctgac
    31501 gctccagcgg tataaaccag cgagaatctt tcaatgccgt gaccagcatt gccgtggcgc
    31561 tttgcggaac agcagtggag aagttacttg ccgggtaggg tttaaattgc ccggtttcgt
    31621 cctgaatgtt gtataccgaa acaaagatct tacccgtcgg cgctggcaga tgggtcaaat
    31681 ctttgtagct ctgagctcga ggcattaatg tcggtctggc ggcttcttta ggcggggcgg
    31741 ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc atgattattt
    31801 tccttatgaa gctggggctt aaaaatcggt tgagttattt tgtaaacccg aaacctggat
    31861 ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac tgcaattgac catcgcggtt
    31921 agcaatatca acaatataat cgttggtcac catgcggccc ggtttaccgg tattaatatt
    31981 cgacagtagc ccacctaaaa tttgtgactg gatggcctga gtaaagttat ctaacgctga
    32041 gggtgtttca ataccaaagt catcgttata gctcggatct ttataagagt tttgggcctg
    32101 agcgctattt aataaaaaag cgccattatt tgggttacca ccaaagtttg gattacggaa
    32161 ctggaaagtc atggttccag cccaacttaa tggcgaaata agcatgagta gaactactgc
    32221 atgtttgaca cgcattgcag cctccgaaca attttttatt tagaattcat catgcgccaa
    32281 atcgcccgta cttaataacg cctgatttat ctggcgacga tttagtgctt cttcagtttg
    32341 aatcagtgca aagacgacag ttttctcgaa gtctcttttc aacggaaata aaaaagtctg
    32401 gaaaataacg tcctgattga ccgttatagt gatccagctt ccccatcgtg cactgggcct
    32461 ttcattaatc gttaagttac ccgtatagtc actttcccat ttatcactaa aggctcggta
    32521 aaaatcatgg ccaatagatg aaacagtatg gtcagttagc aatcccggga cttctacctc
    32581 aacggcgtga agattccctg cggcgaacag aaattctgcc gccacaatcc agcgtaaata
    32641 acgtttcatg gctttatcgc ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt
    32701 tttttacggc tatcttcttg aaaagattat aaagatgcgt tttaaccgta ttttcgctga
    32761 tgaacaacga acgagcgatc tcgttattag acgcgccgat acgcagttta ttcaggatct
    32821 ctttttcccg atgagtaagg agggctgatt ccgtgctgtt ataacgatag ttacctgaat
    32881 gcgtaatcaa gtagctggca agcttttgcg taaagtagca ttcgccgcgc aggacgcctt
    32941 gcaacccatt gacaacacgt tcttgatcct ccatggcata aaaaacgccg ttgatatgag
    33001 gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac aatattttga
    33061 tattgttgtt tttcctgctc aaagtatcct gccaataatg gataagcttt ttatccgctt
    33121 ccatcatatc cagaagaata atagagcctg aagatatatc gtc
//
LOCUS       DAZRVY010000035        32840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZRVY010000035 DAZRVY010000000
VERSION     DAZRVY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.035"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHXOX_001695"
     CDS             267..1925
                     /locus_tag="ACHXOX_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR7005183.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHXOX_001696"
     CDS             complement(1922..2878)
                     /locus_tag="ACHXOX_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR7005184.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHXOX_001697"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHXOX_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR7005185.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHXOX_001698"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHXOX_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR7005186.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHXOX_001699"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHXOX_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR7005187.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHXOX_001700"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHXOX_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR7005188.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHXOX_001701"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHXOX_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR7005189.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHXOX_001702"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHXOX_001702"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR7005190.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHXOX_001703"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHXOX_001703"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHXOX_001704"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHXOX_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR7005191.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHXOX_001705"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHXOX_001705"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR7005192.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGQLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHXOX_001706"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHXOX_001706"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR7005193.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHXOX_001707"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHXOX_001707"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR7005194.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHXOX_001708"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHXOX_001708"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR7005195.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHXOX_001709"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHXOX_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR7005196.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHXOX_001710"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHXOX_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR7005197.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHXOX_001711"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHXOX_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR7005198.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHXOX_001712"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHXOX_001712"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR7005199.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHXOX_001713"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHXOX_001713"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR7005200.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHXOX_001714"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHXOX_001714"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR7005201.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHXOX_001715"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHXOX_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR7005202.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHXOX_001716"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHXOX_001716"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR7005203.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHXOX_001717"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHXOX_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR7005204.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHXOX_001718"
     CDS             29803..30951
                     /locus_tag="ACHXOX_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005205.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHXOX_001719"
     CDS             31161..32591
                     /locus_tag="ACHXOX_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR7005206.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32840)
                     /locus_tag="ACHXOX_001720"
     CDS             complement(32592..>32840)
                     /locus_tag="ACHXOX_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7005207.1"
                     /translation="DDVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8057 a         8033 c         8814 g         7936 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag ctggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatcatc
//
LOCUS       DAZRVY010000036        32648 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZRVY010000036 DAZRVY010000000
VERSION     DAZRVY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.036"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            78..1580
                     /locus_tag="ACHXOX_001721"
     CDS             78..1580
                     /locus_tag="ACHXOX_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR7005208.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            1824..2084
                     /locus_tag="ACHXOX_001722"
     CDS             1824..2084
                     /locus_tag="ACHXOX_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR7005209.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            2275..2415
                     /locus_tag="ACHXOX_001723"
     CDS             2275..2415
                     /locus_tag="ACHXOX_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7005210.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(2722..3021)
                     /gene="ihfA"
                     /locus_tag="ACHXOX_001724"
     CDS             complement(2722..3021)
                     /gene="ihfA"
                     /locus_tag="ACHXOX_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR7005211.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3026..5413)
                     /gene="pheT"
                     /locus_tag="ACHXOX_001725"
     CDS             complement(3026..5413)
                     /gene="pheT"
                     /locus_tag="ACHXOX_001725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR7005212.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5428..6411)
                     /gene="pheS"
                     /locus_tag="ACHXOX_001726"
     CDS             complement(5428..6411)
                     /gene="pheS"
                     /locus_tag="ACHXOX_001726"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR7005213.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6620..6744)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6694..6738)
                     /gene="pheM"
                     /locus_tag="ACHXOX_001727"
     CDS             complement(6694..6738)
                     /gene="pheM"
                     /locus_tag="ACHXOX_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR7005214.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(6861..7217)
                     /gene="rplT"
                     /locus_tag="ACHXOX_001728"
     CDS             complement(6861..7217)
                     /gene="rplT"
                     /locus_tag="ACHXOX_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR7005215.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7270..7467)
                     /gene="rpmI"
                     /locus_tag="ACHXOX_001729"
     CDS             complement(7270..7467)
                     /gene="rpmI"
                     /locus_tag="ACHXOX_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR7005216.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7564..8106)
                     /gene="infC"
                     /locus_tag="ACHXOX_001730"
     CDS             complement(7564..8106)
                     /gene="infC"
                     /locus_tag="ACHXOX_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR7005217.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8110..10038)
                     /gene="thrS"
                     /locus_tag="ACHXOX_001731"
     CDS             complement(8110..10038)
                     /gene="thrS"
                     /locus_tag="ACHXOX_001731"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR7005218.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10563..12461
                     /gene="espL1"
                     /locus_tag="ACHXOX_001732"
                     /pseudo
     CDS             10563..12461
                     /gene="espL1"
                     /locus_tag="ACHXOX_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12633..12740
                     /gene="yniD"
                     /locus_tag="ACHXOX_001733"
     CDS             12633..12740
                     /gene="yniD"
                     /locus_tag="ACHXOX_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR7005219.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12793..13551)
                     /locus_tag="ACHXOX_001734"
     CDS             complement(12793..13551)
                     /locus_tag="ACHXOX_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR7005220.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            13838..14767
                     /gene="pfkB"
                     /locus_tag="ACHXOX_001735"
     CDS             13838..14767
                     /gene="pfkB"
                     /locus_tag="ACHXOX_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR7005221.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            14868..15158
                     /gene="ghoS"
                     /locus_tag="ACHXOX_001736"
     CDS             14868..15158
                     /gene="ghoS"
                     /locus_tag="ACHXOX_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR7005222.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            15264..16124
                     /locus_tag="ACHXOX_001737"
     CDS             15264..16124
                     /locus_tag="ACHXOX_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR7005223.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(16165..16701)
                     /locus_tag="ACHXOX_001738"
     CDS             complement(16165..16701)
                     /locus_tag="ACHXOX_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR7005224.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            16848..17516
                     /gene="hxpB"
                     /locus_tag="ACHXOX_001739"
     CDS             16848..17516
                     /gene="hxpB"
                     /locus_tag="ACHXOX_001739"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR7005225.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            17679..18269
                     /locus_tag="ACHXOX_001740"
     CDS             17679..18269
                     /locus_tag="ACHXOX_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7005226.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            18402..19793
                     /gene="tcyP"
                     /locus_tag="ACHXOX_001741"
     CDS             18402..19793
                     /gene="tcyP"
                     /locus_tag="ACHXOX_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR7005227.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(19797..20600)
                     /locus_tag="ACHXOX_001742"
     CDS             complement(19797..20600)
                     /locus_tag="ACHXOX_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005228.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERFESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFAAIQK
                     NESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(20889..21152)
                     /gene="cedA"
                     /locus_tag="ACHXOX_001743"
     CDS             complement(20889..21152)
                     /gene="cedA"
                     /locus_tag="ACHXOX_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR7005229.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            21335..23596
                     /gene="katE"
                     /locus_tag="ACHXOX_001744"
     CDS             21335..23596
                     /gene="katE"
                     /locus_tag="ACHXOX_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR7005230.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(23854..24612)
                     /gene="chbG"
                     /locus_tag="ACHXOX_001745"
     CDS             complement(23854..24612)
                     /gene="chbG"
                     /locus_tag="ACHXOX_001745"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR7005231.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(24625..25977)
                     /gene="celF"
                     /locus_tag="ACHXOX_001746"
                     /gene_synonym="chbF"
     CDS             complement(24625..25977)
                     /gene="celF"
                     /locus_tag="ACHXOX_001746"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR7005232.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(26082..26924)
                     /gene="chbR"
                     /locus_tag="ACHXOX_001747"
     CDS             complement(26082..26924)
                     /gene="chbR"
                     /locus_tag="ACHXOX_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR7005233.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(26932..27282)
                     /gene="chbA"
                     /locus_tag="ACHXOX_001748"
     CDS             complement(26932..27282)
                     /gene="chbA"
                     /locus_tag="ACHXOX_001748"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR7005234.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(27333..28691)
                     /gene="chbC"
                     /locus_tag="ACHXOX_001749"
     CDS             complement(27333..28691)
                     /gene="chbC"
                     /locus_tag="ACHXOX_001749"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR7005235.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(28776..29096)
                     /gene="chbB"
                     /locus_tag="ACHXOX_001750"
     CDS             complement(28776..29096)
                     /gene="chbB"
                     /locus_tag="ACHXOX_001750"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR7005236.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(29395..29733)
                     /gene="osmE"
                     /locus_tag="ACHXOX_001751"
     CDS             complement(29395..29733)
                     /gene="osmE"
                     /locus_tag="ACHXOX_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR7005237.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            29935..30762
                     /gene="nadE"
                     /locus_tag="ACHXOX_001752"
     CDS             29935..30762
                     /gene="nadE"
                     /locus_tag="ACHXOX_001752"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR7005238.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            30992..31879
                     /gene="cho"
                     /locus_tag="ACHXOX_001753"
     CDS             30992..31879
                     /gene="cho"
                     /locus_tag="ACHXOX_001753"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR7005239.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(31839..32414)
                     /gene="ves"
                     /locus_tag="ACHXOX_001754"
     CDS             complement(31839..32414)
                     /gene="ves"
                     /locus_tag="ACHXOX_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR7005240.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         8659 a         8095 c         7432 g         8462 t
ORIGIN      
        1 gtaagaatca tgctttgata accaattgat aactattatt atattaatct aacaccattg
       61 acatagttga ggtttgaatg aacaaaaagc tgtatttatt cccagactca aacatttttc
      121 tacaatgcaa ggacttgacg caggtaaatt tttccgagat tactgcttgt gatgaagtat
      181 gtatcattat tacaaggccc attcagcaag agattgaccg ccagaaaggg caaggtaatt
      241 cccgcctatc caaaaaagca aggaaggctg ctagcctatt taatcaagta gttgattcaa
      301 ctgacatgac tcttgttatt cgtgaacgct cgccgcgagt ttttcttacc atggatctaa
      361 gcctgaaacc atgtgaacag ttatctgaac aattggatta tcaggaggcc gacgatcgtt
      421 ttgttggtat tgcggcaggg tattgcgctg acgatccaga ttcggaggtg gcaatcatta
      481 ccaatgatag cggtccacgt ttttcagcaa taaaacataa tataatctgc tataaagtgc
      541 cttcttcatg gttgcttcct gctgagccag atgaaaagga taaacaaatt aaattgctgg
      601 aagctaagct gaagcaattc actcagtcta tgcccgattt cagcattaaa gtagccgatg
      661 acgagcaatt aaatattgta cttccttatt atacagcttt atctgaatat gaagttgata
      721 atttgctttg tcaactaacg gatgcattcc cattggttac aaactttgac aatgaacaaa
      781 ctaccccaca atcactagaa aaaatcatca agtcatttag tcaacaggag tatttccctg
      841 tatcagcaga tgatatttct ctttataaag aaaaacatta tccactttgg aaaaagcagt
      901 gtgaagaaat actgaaaaac tgtcatactc atattaatcc gaaggcaata aattacccgc
      961 tgtcgttttt attacaaaac acaggttata ctatcgctga acgtgctgtg gtaacattta
     1021 cagcaaaagg agattttcgt ctgtgtggat ttaattggga gataatggaa gagctgactg
     1081 aaagcaaaag gattgaccta ccttcagccc ctaccgcccc caaaggaaaa tggcgtagtg
     1141 tatgtggcgt aacagaaaga atgccttacg ctgggggagc tttggaaaca ccgctatcca
     1201 gtataatttc aaaagaaatt ggtcctttgt tatctcttac tccaacagaa agggataaaa
     1261 acgattttta ttataaagaa aagagaaatg agccagttca agttgtatcg ctggaatgcg
     1321 aagagtggcg tcatcaagtc aaagaagaaa tattcccgtt gttcgtatat acagccaaca
     1381 aaccaaagaa agtgaatggt gcaattgagg tgagagttga tgcaaataac cttaccaatc
     1441 cggtcattaa aacatttaaa ttaaaaataa ctatagaaga acatagtgca ttttcaacca
     1501 taacagaaat ggtgcagaca tacattcaag agcagaagaa aagacagaaa agttctatta
     1561 gcgcacaaat caaaacatga gggtgtactc aaataataca atgctatagt aaatctataa
     1621 gtgcataact atttcctgat cttttcccaa aatagatgac aggataaaaa acattccaat
     1681 cttttacagg ctttggataa atcgtatcaa agcctatttc ttcaagagtt aaatcaccgc
     1741 ttttcgctcg aaacggactg gctgttggag tatgagccag aacaacttag aatagcccct
     1801 gcactacgtt aagggaggtc gctatgttcc gttgtccgct ttgtggcgca tctgcccgta
     1861 tccgcaccag tcgtccggaa aatgattcaa acaccgtgcg gcaaaagtat taccagtgta
     1921 acaatctgga atgcggcgta tgcttctcaa cactggaagc tttccataaa ttcacatcaa
     1981 aacacgcctc cggcgttcac tcttcagaag atatcccgtg gcatgatctg ccagcttcac
     2041 acaggggaaa caatcagatg agcttgcctt tacctcagaa ttaacaggca gaattgccgg
     2101 agtaacaaaa aagcgataga ttacgcgcgg gtgcctttcg gctgatggtc ggagggaata
     2161 cccgaaggcc agatgtggaa aggccccgga aaacatctct gtttaaccga ggccctaacc
     2221 gcattacctt gacaagtgaa aggttagcgc ctctccggaa aaggagcaag tgctatgtcg
     2281 caaaaatcgc ttacgaccat cacgttctgc gtgacggcaa tcctcatcat ctggatgttg
     2341 cacggttcgc tgtgtgaaat acggatgagc ttctggggag cggagtttgc ggcgttctta
     2401 cagtgtaagc agtaaggaaa ccgcgacggg ggagcaatcc cccgtcaatc ggttgccagg
     2461 gtaaggccgg ataaggcacc ctatctcaca gatatgaaca acaaacccgc agcgtaaaaa
     2521 ctgcgggttt tctttttggt gccctcaccg acttgaaaca gtgataagac tataagacaa
     2581 agcccgcaat gtagcgggct tactcccctc accctccact gaatgtggac acacagtgga
     2641 acgaagcggc ataagatatt ataaatcaat cagttattga aagaaaaaag gccgcagagc
     2701 ggccttttta gttagatcag attactcgtc tttgggcgaa gcgttttcga cccggctttt
     2761 taacttctgc ccgggtctga aggtcaccac gcgccgtgct gtaatgggaa tatcctcgcc
     2821 cgttttcggg ttacgtcccg ggcgttgatt cttatcacgc agatcgaagt taccaaaacc
     2881 agagagtttc acctgttcgc cgttttccag agcgcgacgg atctcttcga aaaacagttc
     2941 aaccagttct ttggcatccc gcttgctaag cccaagctta tcaaacagat attctgacat
     3001 ttcagctttt gtaagcgcca taggttcaat ccctcaatga tgcctggaat cgctctttta
     3061 atgcctctac acatttggcg acggtagcgg caatctcctc ttcttcgagt gtacggctgg
     3121 tatcttgcag gatcaggctt atggcgaggc tcttataccc ctccgcaaca cccttaccgc
     3181 ggtacacgtc aaataagttt acgccaacta cctgatttac gccaactttc ttacattcgg
     3241 ataaaatatc cgctgcggga acgttttctg cgaccaccac cgcgatgtca cgacggttcg
     3301 ccgggaagcg agaaatctcg cgcgcctgag gcaccacgcg gtctgcgagc ttgttccact
     3361 ccagttcgaa caccagagtg cgaccgttaa gatccagttt acgttccagt tcaggatgaa
     3421 caactccaac aaaaccaata cgttcccctt tcagataaat cgctgcggat tgccccggat
     3481 gcagtgccgg attcgcttct gcacggaact caacctcatt cagtttaccg gtcaggtcga
     3541 gaacggattc aagatcgcct ttcaaatcat agaaatcaac ggtctctttt gccaggttcc
     3601 agtgctcttc gtaacggtta ccgcaaatca caccggctaa catcagatcc tgacgaatgc
     3661 ccaacggtgc ctgagtatct ggtacgaaac gcagaccgct ttcgaaaatg cgcacacggt
     3721 tctgctgacg gttctggttg tacaccacgg ttgccagcag gccggtccac agagaaagac
     3781 gcattgctga catttcaaca gagatcgggc ttggcagcag taaggcttca acgcctggat
     3841 ggatcatctg ctgcactttc ggatcaacga agctgtaggt gatcacttcc tgatagcctt
     3901 tgtcgttgag cagcgttttc acgcgcttga gcgacaggtc agcttcacgg tgagtaccca
     3961 taatcaggct tgcctgtacc ggctcatccg ggatgttgtt gtagccgtaa acacgcgcga
     4021 cttcttcaac cagatcttct tcaatctcca tatcgaaacg ccagctcggc gcaactgcct
     4081 gccactcgtc tttgccttcg gtcacttcgc agccgagacg acgcagaatg tcagttacct
     4141 gttcatcagc aatatgatgg ccgatcaggc gatccagttt gctacgacgt aaagtgatgg
     4201 ttgcacgctt cggcagcgtt gcttcgttgg tgatatcaat taccggacca gcctcaccac
     4261 cgcagatgtc gatcagcaga cgggtcgcac gttccatcgc tttgtgctgc agtgccggat
     4321 caacgccacg ctcataacgg tgagacgcat cagtatgcag gccatgacga cgagcacgac
     4381 cggtgataga cagcgggctg aagaaagcgc attccagcaa gacgttttgc gtttcgtcat
     4441 tcacgccaga gtgttcgcca ccaaagatgc cgcccatcgc cagcgccttg ttgtggtcgg
     4501 cgatgaccag agtgtcagca ttcagcttcg cttcagtgcc atcaagcagc accagcgttt
     4561 cgccctcttt tgccatccgc accacaatgc cgccttcaat gcgatcttta tcgaaagcgt
     4621 gcatcggctg gcccagttcg agcagtacat agttggtgac gtcaacaact gcatcgatag
     4681 aacggatccc gcaacgacgc agtttttctt tcatccacag cggagttggc gctttaacgt
     4741 taatgccttt taccacacgg ccaagatagc gcgggcaggc ttcaggcgct tcgactgtaa
     4801 tcggcaacgt gtcgtcgatg gtcgcaccaa ccggaacgat ttccggttca accagcggca
     4861 gctgattcag cacggcaacg tcacgcgcaa caccaatgat acctaagcag tcggcacggt
     4921 ttggcgttac gctgatttcg atggtgttgt catcgagttt caggtattcg cggatgtcag
     4981 tgccaatcgg cgcatctgca ggcagttcga taatgccgct gtgatcgtca gaaattccca
     5041 gttcagagaa ggagcacagc atcccttcag acggttcgcc acgcagtttc gccgctttaa
     5101 ttttgaaatc acccggcaga acagcaccaa tggtagctac cgccacacgc aggccctgac
     5161 ggcagtttgg cgcaccacag acgatgtcca gcaggcgatc gccgccgaca ttcacttttg
     5221 tcacacgcag tttgtcagcg ttcggatgct gcgcacactc aaccacttca ccaacgacca
     5281 cgccgtggaa gctaccggca accggttcta caccgtcaac ttccaggccc gccatagtga
     5341 tttgattcgc cagcgcatcg ctatcaatcg ccgggttcac ccattcgcgt aaccacagtt
     5401 cactgaattt cataatctat tcctgcctta tttaaactgt ttgaggaaac gcagatcgtt
     5461 ttcgaagaat gaacgcaggt cggtgacgcc gtaacgcaac atagtcagac gctccatccc
     5521 catcccgaag gcgaaaccag agtaaacttc cgggtcgatg ccaacgttac gcagcacgtt
     5581 cggatgcacc atcccgcaac ccagcacttc cagccattta ccgtttttac ccatgacgtc
     5641 aacttctgca gaaggctcgg tgaacgggaa gtaggaagga cggaagcgaa tctgcaaatc
     5701 ttcctcaaag aagttacgca ggaaatcgtg cagagtgcct ttcaggttgg taaagctgat
     5761 gttggtatca acaatcagac cttccatctg atggaacatc ggcgtgtgag tctggtcgta
     5821 gtcgttacga taaacgcggc caggcgcgat gatacgaatc ggtggctgct gggctttcat
     5881 ggtgcggatc tgtacgccag aggtctgggt acgcagcagg cgggtagtgt caaaccagaa
     5941 agtgtcgtgg tcagcgcgcg ccgggtggtg accaggaatg ttcagagcat cgaagttatg
     6001 ataatcgtct tcgatttccg gcccggttgc cacggtaaag ccaagctcac cgaagaaact
     6061 ttcgatacgg tcgatggtac gggtaaccgg atgcagaccg ccgttttcaa tgcgacgacc
     6121 tagcagggag acatcaatcg tttccgccgc cagacgcgca ttcagcgctg cgctttccag
     6181 ttccgcttta cgcgcattca gcgcctgctg aacctgctct ttcgcttcgt tgataaccgc
     6241 accagctgcc ggacgctctt ctggcggcag ctcacgcagg gtcgtcatct gaagggttaa
     6301 gtgccctttt ttacccaaat attcgacgcg cacattatct aacgcggcaa catctgacgc
     6361 ctggctaatg gccgccttcg cactggcaac cagttctgcg agatgtgaca tggttttcct
     6421 cattgtgtca gtggtgacac tggttcgttg gacttagagc ctatcccatc aggctatttt
     6481 acttgccatt ttggtcccgg gcagtgctcg aaattctcac gtacttaaat acgctccggt
     6541 ttctccgcgc tggccgtgtc cagactggct gcgacaatta cgcctgatag actggctttt
     6601 tatttttcaa aacgcgcata caaaaaaagc ctccactggg aggctttcag gcgctgtttt
     6661 ccgtttctct tctcacgcgc tagcctcctg gattcaggtg ctaaagtaaa aaaagaagcg
     6721 gaaaatagca gcattcattg cttgcgttac cttttggtac tcttcaaaag acctttattg
     6781 aaaaggctac ggcgataaaa gtcaatgttt tgatggcgtt gaaacgaaaa gagggagact
     6841 agctccctct ttcaactggc ttatgccaga gctgctttcg ctttttcaac cagagcggtg
     6901 aacgctactt tgtcgaatac tgcgatatca gccaggatct tacggtcgat ttcaacagag
     6961 gcttttttca ggccattgat gaatttgctg taagaaatac cgttctgacg tgctgctgcg
     7021 ttgatacgcg caatccacag ttgacggaac tgacgcttac gttgacgacg gtcacggtaa
     7081 gcatactgac cagctttgat aacagcctgg aaggcaacgc ggtatacgcg agaacgcgca
     7141 ccgtagtagc ctttagcttg tttcaaaatt ttcttgtgac gtgcgcgtgc aataacacca
     7201 cgttttacgc gagccatatg tgctctcctg tatctatatt ctaattaaaa agttaaaaac
     7261 gttaacggct tatgcgtacg gcaggcacgc gattaccagg cccagatcgc ctttggaaac
     7321 catggctttc ggacgcaggt gacgtttacg tttggtcgct tttttggtca gaatgtgacg
     7381 caggttagcg tgcttgtgct taaaaccacc tttaccggtt tttttgaagc gcttagcagc
     7441 accgcgtacg gtcttaattt ttggcatttt aataacttcc acttcgcatt gttaataaac
     7501 gaaacaaagg cgaacaaagc ctgtgaagcc cgaaggctcc acagacagtg ctacttgaag
     7561 gccttactgt ttcttcttag gagcgagcac catgatcatc tggcggcctt cgatcttcgt
     7621 tgggaaggat tcgaccactg ccagttcttg caaatcgtct ttcacgcgat taagcacttc
     7681 cataccgatt tgctggtgcg ccatctcacg accgcggaaa cgcagcgtga ttttggcttt
     7741 atcaccctct tcgagaaagc gaatcaggct gcggagtttt acctgatagt cgccttcatc
     7801 tgtaccagga cggaatttaa tttccttaac ctggataact ttttgctttt tcttctgttc
     7861 cttagaagac ttgctctttt catagaggaa tttgccgtaa tccattatac gacaaaccgg
     7921 cggctcggcg ttagggctga tctcgactaa gtctactccg gcttcttctg ctttctccag
     7981 agcttctctc agactcacaa taccaagctg ctcgccttcc agacctgtta agcgaacttc
     8041 ctgggcgcga atttcgccat tgatacggtt agggcgcgcc gtttgaactc gttttccgcc
     8101 tttaatacct tattcctcca attgtttaag actgcggctg cgaatctctt gttgcagctt
     8161 ctcgatcact tcatttacgt ccatgcttcc caggtcttta ccacggcggg tgcgaacggc
     8221 aactttgcct gattccacct ctttatcacc acagaccagc atatatggga cgcgacgcaa
     8281 agtgtgctcg cggattttaa agccaatctt ctcatttctc aagtctgctt taacacgaat
     8341 gcccgcattt gatagttttt gcgtcaattc gttaacgtat tcagactgtg aatcggtaat
     8401 attcatgata acaacctgaa ctggcgcaag ccaggtcggg aagaaaccag cgaattcttc
     8461 ggtcaggata ccgatgaaac gttccatcga ccccagaatt gcgcggtgaa tcattaccgg
     8521 taccttacgc tcgttgtctt cgcccacata ggaggcgctc agacgagacg gcaaggagaa
     8581 gtccagctgt actgtaccgc actgccatgc acgatcgagg cagtcataca gggtaaattc
     8641 aattttcgga ccgtagaaag cgccttcacc cagttgatat tcaaacggga tgttgttttc
     8701 ttccagcgca accgccaggt ccgcctcagc acgatcccac atttcgtcgc tgccaatacg
     8761 tttttcagga cgagtggaga gtttgacgac gatcttctcg aagccaaaag tgctgtacat
     8821 atcatagact aaacggatac atccgttaac ttcatcgcga atttgttctt cagtacagaa
     8881 gatatgcgcg tcatcctggg taaatccacg cacgcgcatc aggccatgca gcgaacctga
     8941 cggctcgtta cggtggcagc taccaaactc ggccatacgc agcggcagat cgcgataaga
     9001 cttcagcccc tggttgaaaa tttgtacgtg acccgggcag ttcatcggct taatgcagta
     9061 ttcacggttc tcagaagatg tggtgaacat tgcatctttg tagttgtccc agtgaccggt
     9121 tttttcccac aggacacggt ccatcatgaa cggaccttta acttcctgat actggtactc
     9181 tttcagttta gaacgaacaa acacttccag ttcacggaag atggtccagc cgtcgttgtg
     9241 ccagaatacc atacccggcg cttcttcctg catatggtac aggtcgagct gtttaccgat
     9301 tttacggtgg tcgcgtttcg cggcttcttc caggcgctgc aggtaagcgt taagtgcttt
     9361 tttgtctgcc cacgccgtac cgtaaatacg ttgcaacatt ttgttgttgc tgtcgccacg
     9421 ccagtaagcc cctgccgttt tcattagttt gaaatgatgg cagaaacgca tgttcggtac
     9481 gtgcggaccg cggcacatat cgacatattc ttcatggaag tacagacctg gcttgtcatc
     9541 atgggcgatg ttttcgtcaa gaatggagac tttgtagctc tccccacggt tggcgaaagt
     9601 ttcacgcgct tcgtgccagc tgactttctt cttaatgacg tcgtagtttt tctcagcaag
     9661 ctcatgcatc cgcttctcga gtgcttcgac atcttcctgg gttaacgtgc ggtcaagatc
     9721 aacgtcgtaa taaaaaccgt tgtcaataac cgggccgatt gccattttgg tatgcggcca
     9781 aagttgttta atcgcatgcc ctaacaggtg cgcacaggag tgacgaatga tctccagacc
     9841 ttcttcgtct ttggcggtaa tgatcgacag ttgtgcgtcg ttttcaatca gatcgcaagc
     9901 atcaaccagt tcgccattaa cgcgccctgc gatacaggct ttcgccagac ctggaccaat
     9961 gtccagcgca acatccatgg ggcttacagc gtggtcgtaa tggcgttggc tgccatcagg
    10021 aagagttata acaggcattt tatatcctta atttgcagtg gtgacccaca cgaaagatca
    10081 catacaaaga aaaatttgtt tattaacagt tcattgcgaa accatctagc caacaaatgc
    10141 taaattggtt cgcaatcagg tacacaaatg aaagacatta acctctcacc ttccacccgg
    10201 ttgaacgatg ttaacactaa aaaaagggag attgtacctt tccgtttcac attgattcga
    10261 ttcgcaattc gtttgtagtg agtacaccag caaaacgctt aaagaaccac tgataacatc
    10321 acggtgcgtc tgaaaggttt actgcgagcg gatttacagc agcataaact gaatccagca
    10381 aagttcgttg cgtgatttgg cggtagtatt ttaaacgctc atgagattaa tgacgaagtg
    10441 gtcatatcac aatgataaaa gtgacacaat tcttatagca atttttcgtg cacatttcgt
    10501 tctggcgata ataattaatc atcatcctca aaacctccag acatatataa ggcgaataga
    10561 ttatgagtca aaacgatatc attatcagaa cccattataa gtctcctcat agattgcaca
    10621 tcgatagcga cataccaacg ccttcatcag agcctattaa tcaatttgcg cgccaactca
    10681 tcaccctact tgatacctct gacttaagtt cgatgctgtc atactgtgtt actcaggaat
    10741 ttaccgcaaa ctgtcgaaaa atttcacaaa attgttattc cactgccctt tttatcatta
    10801 actttgccac ttcacccatc catgcagaaa atatactcat tacattacac tataaaaaag
    10861 aaatcattcc cttatttctg gaaaccacgc ctattaaggc taaccatttg cgaagcatac
    10921 tggattatat tgaacaggaa cagttaactg ccgaagatcg taaccattgt atgaaactgt
    10981 ctaaaaaaat ccatagagaa aaaactatac aaccaacagt aaatctcaat ggtagtgcat
    11041 ttttttcgca atctccttct gacgctattt tttgtcgcca tctgtcattg caatacgccc
    11101 ttgattcatt gagaaatgga aaaggcaaag tcaacctgat taaacactac tcctccgttg
    11161 aatccataca gcagcatgtc cccttagtcc gggacgcgga gttcagagca ttacttcgcc
    11221 atcctcctgc agggagtcgc gttatcgcga gtaaggattt tggcttcgct ttagatattt
    11281 tcttttgtcg aatgatggca aacaatgtca gtcatatgtc cgcgatttta tatatagaca
    11341 atcatacttt gtcagtaagg ctacgaataa agcagtcagt gtatggacaa ttaaattatg
    11401 ttgtgtctgt ttacgacccg aacgatacca acgttgccgt cagaggcacc cacagggcag
    11461 cacggggctt tctctcgctt gataagttca tcagttcagc tcccgatgct cagacctggg
    11521 ctgataggta tgttcgcaac tgtgcaattg ctattcttcc tctattacct gagggagttc
    11581 cagttgctat tttagcgggt atcacgacac gaatgccatt tgcccctata catccatcgg
    11641 caatgttgtt aataatggcc acaggccaga ctcaatagct tattacacta ttcaaacagt
    11701 tgcccatact ccctgaaaaa gaaatcattg aaataataac tgcgcagaat agcgttggta
    11761 cacctgcttt atttctggct atgatgaacg gacaaactga caacgtaaaa atatttatgc
    11821 aagaaattca gtcactggta tacaatcata tcattcatga agataatctg gttaaattac
    11881 tgcaaactaa aagtgctaac gaaacacctg gactctatat ctccatgttg tatggattcg
    11941 atgaaataat cgatatcttt ctgaatgcat taaccactcc aatagcacaa gaacttttaa
    12001 acaaaaaaat ggtgatgagt attttagcca tgaaaataca tgatggtgag ccaggattat
    12061 acgccgcaat ggaaaataat caccctttgt gtgtcacacg gttcctctct aaaattaatg
    12121 gcatcgcctt taaatacaag ttgagcaaag ctaacatcat ggatttatta aaaggagcta
    12181 cagcacaggg aacccctgca ttatacatcg ccatgagcaa gggtaatgaa gacgtcgtgt
    12241 tatcttatat atcgacgctg ggtgcttttg caaaaaaaca ttcttttagt caacatcagt
    12301 tatttacact attggccgct aaaaatcatg acaacatgtc agctgttcat atagccattc
    12361 atcataagca ttataaaact gtagaaacat attatgctgc tataaatgtc atcagccaaa
    12421 gcctgagttt tagtgctgat gaaataaaga cgtatttata accagctatt tacgatatat
    12481 atcgatacat aattttattt ttttcataaa acgcagaaac agtgaccatg tgcacaacgc
    12541 cacccgacgg ctgtaataac aaaaaatccc ttgatgccta tcccttttgt tacactccgt
    12601 tatcacgcac aagagatatg caggacactg gtatgccgac taaacgcttt gataaaaaac
    12661 actggaagat ggtggtggtg ctactggcaa tctgtggcgc tatgttgttg ctacgttggg
    12721 cagcaatgat ttggggctga gcagtgacaa acgaagacag ccagaccgca taacacggtc
    12781 cggcagataa ttttacatgc tgtagcccag cgataaggtt gtccgacgat cggtatgttc
    12841 cggtgcagac tctggaggtt cagagttcca tgtgacgttg taagccacct tcagtccaaa
    12901 atgttcatta atagcaacat ttaatgcgct ttcggagttc agcgttgtat cttccgcgcc
    12961 aaagaccgaa acaccctgcg taaatttagc gttgtcagtc aactgccatg cataagcccc
    13021 ggaggcatag cccagcggct gggtttcact ggcattgtcg gtatatttgt cgtaacgcac
    13081 acctgggccg aattcaaagc ggaaactgtg taccgggcca ttcaaaaact ggcgaccata
    13141 acccgcggtc aacacatcgc gctctcgata gccgttataa cggtctgtca gccagcttgc
    13201 ctgaccaaat aaatagtcat aatcagttaa attaaaacgg ctacgcccgc ccgccgcata
    13261 tttttctgaa gaacgctcat cattagaaga agtattactg gcgttccccc acagcgacca
    13321 ggcagtggtt tgtccatacc aggtcatggt ggtgtcagcc gtaagtgagg agcttttcgt
    13381 attgcctgat tgtgcaagat atcctgcgtt cagattacct tcgaaaggtt ttttagcgct
    13441 ggcaggatcg tccatgacag taaaaacgga atcatcggca gctgcattca gtgacgcaaa
    13501 catgcccccc gccaacataa cgatggcagg aactgtcttc aaaagcttca tttatcaaga
    13561 gtccgtacaa caaaaaaaga gaccatcgcg gtcccggaaa ctttcttaag gatcaaagat
    13621 tagcgtccct ggaaaggtaa cgaattataa aaaggcgcga ataacttagc aatgtattct
    13681 tatttcattt tttgaataag catgtggcga aaacagattt ttatttatat atatttatct
    13741 gcaaaatttt aaataaagct ccaataaatc atattgttaa tttcttcact ttccgctgat
    13801 tcggtgccag actgaaatca gcctatagga ggaaatgatg gtacgtatct atacgttgac
    13861 acttgcgccc tctctcgata gcgcaacaat taccccgcaa atttatcccg aaggaaaact
    13921 gcgctgtacc gcaccggtgt tcgaacccgg gggcggcggc atcaacgtcg cccgcgccat
    13981 tgcccatctt ggaggcagtg ccacagcgat cttcccggcg ggtggcgcga ccggcgaaca
    14041 cctggtttca ctgttggcgg atgaaaatgt ccccgtcgct actgtagaag ccaaagactg
    14101 gacccggcag aatttacatg tacatgtgga agcaagcggt gagcagtatc gttttgttat
    14161 gccaggcgcg gcattaaatg aagatgagtt tcgccagctt gaagagcaag ttctggaaat
    14221 tgaatccggg gccatcctgg tcataagcgg aagcctgccg ccaggtgtga agctggaaaa
    14281 attaacccaa ctgatttccg cttcgcaaaa acaagggatc cgctgcatcg tcgacagttc
    14341 tggcgaagcg ttaagtgcag cactggcaat tggtaacatc gagttggtta agcctaacca
    14401 aaaagaactc agtgcgctgg tgaatcgcga actcacccag ccggacgatg tccgcaaagc
    14461 cgcgcaggaa atcgttaata gcggcaaggc caaacgggtt gtcgtttccc ttggtccaca
    14521 aggagcgctg ggtgttgata gtgaaaactg cattcaggtg gtgccaccac cggtgaaaag
    14581 ccagagtacc gttggcgctg gtgacagcat ggtcggcgcg atgacactga aactggcaga
    14641 aaatgcctct cttgaagaga tggtccgttt tggcgtagct gcggggagtg cagccacact
    14701 caatcaggga acacgtctgt gctcccatga cgatacgcaa aaaatttacg cttacctttc
    14761 ccgctaacaa aaacattccc ccagcattgg gggaatcacc accaacctgt cggcaacgcg
    14821 tttctccgac tttgctcaaa agtcatgtga taacaaaggg gtgaactatg gccagtggcg
    14881 atcttgtccg ttatgtcata accgtcatgt tgcatgagga tacattgact gaaattaacg
    14941 agttgaataa ttacctgact cgcgacggtt ttttgctcac catgacggat gatgagggaa
    15001 atatccatga gctggggact aacacttttg gacttatcag tacccaaagt gaagaagaaa
    15061 ttagagaact ggtttcgggg cttacccaaa gtgcaaccgg caaagatcct gaaatcacca
    15121 tcacgacttg ggaggaatgg aatagcaaca gaaaataaat ggtttttggg caataatcag
    15181 tctgtggtgt gcgttagctc gtgtttttac accgcattct tgcgctaacc ttatgatctg
    15241 gcagacaaca tgggagagac atcatgtggc aggcaatcag tcgtcttttg agcgagcagt
    15301 taggtgaagg cgaaatcgaa ctgcgtaatg aactgcctgg cggagaagtc catgccgcat
    15361 ggcatttgcg ctatgcagga catgactttt tcgtcaaatg tgatgaaagg gaactgcttc
    15421 ccggttttac cgccgaagcc gaccaactgg agttactgtc gcgtagtaaa accgtcaccg
    15481 tgcctaaggt ttgggcagta ggcgctgacc gtgactacag ttttctggtg atggattatc
    15541 tcccacctcg tccgctggat gcgcatagcg catttattct tggtcagcaa attgcgcgtt
    15601 tacatcaatg gagtgaccaa ccacaatttg gcctcgattt tgataactcg ctctccacaa
    15661 ctccacagcc caacacctgg caacgtcgct ggtcaacgtt ttttgctgaa caacggattg
    15721 gctggcagtt ggaactggca gcagagaaag ggatcgcttt cggcaatatc gacgccatcg
    15781 tcgagcatat tcagcagcgt ctggcctcgc atcaactgca gccttctctg ttgcacggcg
    15841 atttatggtc cggcaactgt gcgctgggtc cggatggccc gtacattttc gaccctgcct
    15901 gctactgggg tgaccgagag tgcgacctgg cgatgttacc gctgcatact gaacagccgc
    15961 cacaaatcta tgacggctat cagtcagtat ccccgctacc tgccgatttc cttgaacgtc
    16021 aaccggttta ccaactctac acgctgttaa atcgtgcaag attatttggc ggtcagcatt
    16081 tggttattgc tcagcagtca ttggatagat tattagcagc atgatatggg ttgaggataa
    16141 tggccgctcc gtgcggcctt ttgattaaat aaacccaagc agagaaaaga acacataacc
    16201 tgcagcaata caaataaccg gcaggatata tagtgagaaa aactgcagga aaatagtatg
    16261 gtgcggaaca acaatacggg actcaatttg ttcacgcgtt agcccctctt cccctttggc
    16321 tttttccaga atgagttgat cttcaacacc ttcccgcaag aagcgcgcct ggcgactcat
    16381 ccgggcaccg gaatcctgca acgccagtcc gacaaaaatc aggatgaaaa tcacccagaa
    16441 cataacgttc acgccaccat taaaattagg ggtcggggag ttataccaga acagattcaa
    16501 aaaaggcgta ttcgcctgca tcatatcgat catgacatga gtaaagtcga gcatgaccgc
    16561 attaatgcct tcctggtttt cctgccgggt attcataaat ttcagcaaag aaatcaatgt
    16621 ggagatcaat gcaggtataa aaatcaccca acccaaaatc cttttcaaaa cagcaatgcg
    16681 tccagcttgt tgatacgtca tgagttctcc ttgattaaga cgcgtcgttt cacttagttt
    16741 acctgtagat atctgttttc gcccattctt taaaggcgat atgataggcg cttaatcata
    16801 agcacggctt aataccttac acataatgct ctaaaggaga ggttgtaatg tcaaccccgc
    16861 gtcagattct tgctgcaatt tttgatatgg atggattact tatcgactca gaacctttat
    16921 gggatcgagc cgaactggat gtgatggcaa gcctgggggt ggatatctcc cgtcgtaacg
    16981 agctgccgga caccttaggt ttacgcatcg atatggtggt cgatctttgg tacgcccggc
    17041 aaccgtggaa tgggccaagc cgtcaggaag tagtagaacg ggttattgcc cgtgccattt
    17101 cactggttga agagacacgt ccattattac caggcgtgcg cgaagctgtt gcgttatgca
    17161 aagaacaagg tttattggtg ggactggcct ccgcgtcacc actacatatg ctggaaaaag
    17221 tgttgaccat gtttgactta cgcgacagtt tcgatgccct cgcctcggct gaaaaactgc
    17281 cttacagcaa gccgcatccg caagtatatc tcgactgcgc agcaaaactg ggcgttgacc
    17341 cactgacctg cgtggcgctg gaagactcgg taaatggcat gatcgcttct aaagcagccc
    17401 gcatgcgttc catcgtcgtt cctgcgccag aagcgcaaaa tgatccacgt tttgtattag
    17461 caaacgtcaa actctcatcg ctgacagaac tcaccgcaaa agaccttctc ggttaatgac
    17521 caggggcagt gatcacgctg cccctggttc tttatctgaa tttgcattca actgacggat
    17581 taatcgtcaa tttaagagaa agagttacac cgtcaccact tccgtgcact gtataaaaat
    17641 cctatactgt acgtatcgac agtttagtga gttttatcat gacggcggaa ggtcaccttc
    17701 tcttttctat tgcttgtgcg gtatttgcca aaaatgccga gctgacgccc gtgctggcac
    17761 agggtgactg gtggcatatt gtcccttccg caatcctgac gtgtttgtta ccggacatcg
    17821 atcacccaaa gtcgtttctt gggcagcgat taaaatggat atcaaaaccg atcgcccgcg
    17881 cttttgggca tcgtggtttt acccacagtc tgctggcggt atttgcgctg ctggcaacct
    17941 tttaccttaa ggttccggaa ggctggttca ttccggctga tgcgctacaa ggaatggtgc
    18001 tgggttattt gagccacata cttgccgata tgctgacacc cgccggtgtt cccctgctct
    18061 ggccatgccg ctggcgtttc cgcttgccta tcctggttcc ccaaaagggc aaccaactgg
    18121 aacgttttat ctgcatggca ttatttgtct ggtcggtatg gatgccccat tcattacccg
    18181 agaacagcgc tgttcgttgg tcatcgcaaa tgatcaatac cttgcagatc cagtttcatc
    18241 ggcttattaa gcatcaggtt gaatactaaa aaggcaaaaa tcacctttct ggaataagca
    18301 attccatttg aatataagag ccagctcaca gttctgttaa tcttgcgcca acactatgac
    18361 tgctacgcag tgatagaaat aataagatca ggagaacggg gatgaacttt ccattaattg
    18421 cgaacatcgt ggtgttcgtt gtactgctgt ttgcgctggc tcagacccgc cataaacagt
    18481 ggagtctggc gaaaaaagtg ctggtgggtc tggtgatggg tgtggttttt ggccttgccc
    18541 tgcataccat ttatggttct gacagccagg tacttaaaga ttctgtacag tggtttaaca
    18601 tcgttggtaa cggctatgtt caactgctgc aaatgatcgt tatgccgtta gtcttcgcct
    18661 ctattctgag cgcggttgcc cgtctgcata acgcatctca gttaggcaaa atcagttttc
    18721 tgaccatcgg tacgcttttg tttaccacgc tgattgcggc gctggtcggt gtgctggtca
    18781 ccaacctgtt tggtttgacg gctgaaggtc tggttcaggg tggtgcagaa actgcacgtc
    18841 tgaacgccat tgaaagtaac tatgttggta aagtctctga tctgagcgtt ccgcagctgg
    18901 tcttgtcctt tatcccgaaa aacccgtttg ccgatcttac cggagccaat ccgacgtcaa
    18961 ttatcagcgt ggtaattttt gccgcattcc tcggcgtagc tgcattgaaa ctgctgaagg
    19021 atgatgcgcc gaaaggtgaa cgcgtcttag ccgctatcga taccctacaa agctgggtga
    19081 tgaaactggt tcgccttgtc atgcagttga ccccttacgg cgttctggct ctaatgacca
    19141 aagtggttgc aggttctaac ttgcaagaca tcatcaaact gggaagtttc gttgtcgcgt
    19201 cctacctcgg tctgctgatt atgtttgcag tgcatggcat tctgctgggc attaatggcg
    19261 tgagtccgct gaagtacttc cgtaaggtat ggcctgtgct gacgtttgcc tttaccagcc
    19321 gttccagtgc tgcgtctatc ccactgaatg tggaagcaca aacgcgtcgt ctgggcgttc
    19381 ctgaatccat cgccagtttc gccgcctctt tcggtgcaac cattggtcag aacggctgcg
    19441 ccggtttgta tccggcaatg ctggcggtga tggttgcgcc tacggttggc attaacccgc
    19501 tggacccgat gtggattgcg acgctggtcg gtattgttac cgttagttcc gcaggcgttg
    19561 ccggtgtcgg tggtggtgca actttcgccg cactgattgt gctgcctgcg atgggcctgc
    19621 cagtaaccct ggtggcgctg ttaatctccg ttgaaccgct tatcgacatg ggccgtacgg
    19681 cgctaaacgt tagtggctcg atgacagctg gcacgctgac cagccagtgg ctgaagcaaa
    19741 ccgataaagc cattctggat agcgaagacg acgccgaact ggcacaccgt taattcttat
    19801 gctggcaacg gtccgttttg tataggggcc gttgctttac tttttaaatg ttccatcgcg
    19861 gcgatgcata ctgactcata tttaaacaga tcgtcagcga aaaaaatgtc caacttatca
    19921 atacattcct ggctcaatga atgtgcctgt agtgagaagc ttaaaacctc atacccgaga
    19981 cgttttctaa gtgaactatc tggaatactc ataatatctt caagcacacc acttcccatt
    20041 cccggaagat ttagtggatt aaaaagaaga ttgatattaa gcattgatat tatcgaagga
    20101 aactttagga gactctcatt tttttgaatc gcagcaaaaa tgctcccggc aggatgtata
    20161 taattttttt catagtatat agtccttaaa taatgaataa ccaacctatt ttttatattg
    20221 atcaaattcg tcattttttc gggtcggaat tcacaggtaa aatccatccc aacaaaattc
    20281 tcattaatat ctcttatccg aaactcactg ccataaagat tattaattat cactctatat
    20341 ttaacattgt cctgcagata cgtatcaaaa ctaagtgctg gagctcctgg ctttgaggca
    20401 tcatagttaa tgttagacat ataggcaatc atacgattga agttcgactc tgctatttca
    20461 tcgcagtcac attgacccgt tgatataaag aaagtgagtg ccgccatggt agactcaaac
    20521 ctttctttaa aagagtcgaa agccaggctt ttaatattta aatcaccata attactctgt
    20581 attaagtttg tagaaaacat ctcccgcccc atattgttaa caaaattatt atctcattta
    20641 aatctaagtc acttacaata taagtttaag agcgacgcca caggatgaac tatcaaaaat
    20701 agctcatcat gattagcaaa acttaaccat tttaaaataa ataaacaatt aaagaaaaaa
    20761 gatcacttat ttatagcaat agctcgtcaa aggcagcttt ttgttacagg tggtttgaat
    20821 gaatgtagca acgaaatgca gaatttcagg tcatgtaact cccggcaaaa ccgggaagta
    20881 tgtaatcctt actcagtcac ttccccttcc tggcggatct gatttgccca acgttgggca
    20941 gattcaggca cagtaaacgc cggtgagcgc agaaatgact ctcccatcag tacaaacgca
    21001 acatatttgc cacgcagcat ccagacatca cgaaacgaat ccatctttat cgcatgttct
    21061 ggcggcgcgg gttccgtgcg tgggacatag ctaataatct ggcggttttg ctggcggagc
    21121 ggtttcttca ttactggctt cactaaacgc atattaaaaa ttagaaaaac tgtagtttag
    21181 ccgatttagc ccctgtacgt cccgctttgc gtgtatttca taacaccgtt tccagaatag
    21241 tctccgaagc gggatctggc tggtggtcta tagttagaga gttttttgac caaaacagcg
    21301 gccctttcag taataaatta aggagacgag ttcaatgtcg caacataacg aaaagaacct
    21361 acatcagcac cagtcaccac tacacgattc cagcgaagcg aaaccgggga tggactcact
    21421 ggcacctgag gacggatctc atcgtccagc ggctgaacca acaccgccag gtgcacaacc
    21481 taccgcccct gggagcctga aagcccctga tacacgtaac gaaaaactta attctctgga
    21541 agacgtacgc aaaggcagtg aaaattatgc gctgaccact aatcagggcg tgcgcatcgc
    21601 cgatgatcaa aactcactgc gtgccggtaa ccgtggtcca acgctgctgg aagattttat
    21661 tctgcgcgag aaaatcaccc actttgacca tgagcgcatc ccggaacgta ttgttcatgc
    21721 acgcggatca gcagctcacg gttatttcca gccatataaa agcttaagcg atatcaccaa
    21781 agcggatttc ctctcagatc cgaacaaaat caccccagta tttgttaggt tctctaccgt
    21841 tcagggtggc gctggctctg cagataccgt gcgtgatatc cgtggttttg ccaccaagtt
    21901 ctatactgaa gagggtattt ttgacctcgt tggcaataac acgccaatct tctttatcca
    21961 ggatgcgcat aaattccccg attttgttca tgcggtaaaa ccagaaccgc actgggcaat
    22021 tccacaaggg caaagtgccc acgatacttt ctgggattat gtttctctgc aacctgaaac
    22081 tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc ccccgcagtt accgcaccat
    22141 ggaaggcttc ggtattcaca ccttccgcct gattaatgcc gaagggaagg caacgtttgt
    22201 acgtttccac tggaaaccac tggcaggtaa agcctcactc gtttgggatg aagcacaaaa
    22261 actcaccgga cgtgacccgg acttccaccg ccgcgagttg tgggaagcca ttgaagcagg
    22321 cgattttccg gaatacgaac tgggcttcca gttgattcct gaagaagatg aattcaagtt
    22381 cgacttcgat cttctcgatc caaccaaact gatcccggaa gaactggtgc ccgttcagcg
    22441 tgtcggcaaa atggtgctca atcgcaatcc ggataacttc tttgccgaaa acgaacaagc
    22501 agcattccat ccaggtcata ttgttcctgg tctggacttc accaacgatc cgctgttgca
    22561 gggacgtttg ttctcctata ccgatacaca aatcagtcgt cttggtgggc cgaatttcca
    22621 tgagattccg attaaccgtc cgacctgccc ttaccacaac ttccagcgtg atggcatgca
    22681 tcgtatgggg atcgacacta acccggcgaa ttacgaaccg aactcgatca acgataactg
    22741 gccgcgcgaa acaccgccgg gaccgaaacg cggcggtttt gaatcatatc aggaacgcgt
    22801 ggaaggaaat aaagttcgtg agcgcagtcc atcgtttggc gaatattatt cccatccgcg
    22861 tctgttctgg ctaagtcaga cgccattcga gcagcgccat attgtcgatg gtttcagttt
    22921 tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc gttgttgacc agctggcgca
    22981 tattgatctc actctggccc aggcggtggc gaaaaatctc ggtatcgaac tgactgacga
    23041 ccagctgaat atcaccccac ctccggacgt caacggtctg aaaaaggatc catccttaag
    23101 tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg gtagcgattt tacttaatga
    23161 tgaagtgaga tcggcagacc ttctggccat tctcaaggcc ctgaaggcca aaggcgttca
    23221 tgccaaactg ctctactccc gaatgggtga agtgactgcg gatgacggta cggtgttgcc
    23281 tatagccgct acctttgccg gtgcaccttc gctgacggtc gatgcggtca ttgtcccttg
    23341 cggcaatatc gcggatatcg ctgacaacgg cgatgccaac tactacctga tggaagccta
    23401 caaacacctt aaaccgattg cgctggcggg agacgcgcgc aagtttaaag caacaatcaa
    23461 ggtcgctgac cagggtgaag aagggattgt ggaagctgac agcgctgacg gtagttttat
    23521 ggatgaactg ctaacgctga tggcagcaca ccgcgtgtgg tcacgcattc ctaagattga
    23581 caaaattcct gcctgatggg aggcggcgca attgcgccgc ctcaaatgat ttacatagtg
    23641 cgctttgttt atgccggatg cggcgtgaac gccttatccg gcctacaaaa ctgtgcaaat
    23701 tcaatatatt gcaggaaaca cgtaggcctg ataagcgaag cgcatcaggc agttttgcgt
    23761 ttgtcagcag tctcaagcgg cgcagttacg ccgcctttgt aggaattaat cgccggatgc
    23821 aaggttcacg ccgcatctgg caaacatcct cacttacaca tcccgataac tccccaaccg
    23881 ataaccacgc tgagcgatag cacctttcaa cgacgctgat gtcaacacat ccagctccgt
    23941 taagcgtggg aaacagtaag cactctgacg gatagtatta tcgataaacg ccggatgaca
    24001 catcacctcc agcgaacgat caccccgatg gcttgcgtca tccagcactt gcaggaacag
    24061 tgcctcgcta atctcttcac cataaaacgc gctactgaat ccctgagaac tgcgcagatt
    24121 agccggtaaa tcaccagagt tagatagcgg ctgacggtcg atgcgcaacg caatcccctc
    24181 ttcagccgca aacctggcaa caatcgggaa aatctgcggg aacatatgca catgatgatg
    24241 gctatcaaga tgcgtaggtt tgcgtccaaa tagctcaatg aaacgcaaat actgactggc
    24301 aagctcctga gtaatttctt ccagcggtaa agcatcttct tctgccaact gccagatcca
    24361 ttttcccagc acaccatcgc gggttaaccc cggcatagct gtcagtggct tgcccatagt
    24421 aaggacaaag tacatcccta tggccagact cggttcatca cgactcaact gcaccgcatg
    24481 gtcaatagcc tgcccattca ccagcgccgt cgtcgatgtg acaatcccat tgcgacaggc
    24541 ctcgataatg ccgtagttct gacctttgct taagccaaaa tcatcggcat taacaatcag
    24601 taagcgttcc ataatcagcc tcggttaatg tgctttttta agctctgcga tgcagtcggc
    24661 aaaatttggc agccatttct cgtgcgccag aatcatctcg cgtgccagca gctcagcatc
    24721 gcgatcggaa tgcaccaacg gactaaggtt tagcgccagt aacacatcgt taaattctcc
    24781 gctaagtgcg gcgttgctgg cagcaatctc gaagccttta atggtgtgaa tcagccccat
    24841 cactttatca tcgaaatgcg taatgcgcgg atgtggcgtc gcgccatcgc gccccagctt
    24901 acaggtcatt tctaccgccc agtctgccgg aatattatca atatgcccat gatgcgggat
    24961 attaacgtaa tgttcagctt gcttgtcgtt gtagatagcg ttgatcactt cgcacgctgc
    25021 atcagagtaa taagccccac cgcgctgttc cagttctttc ggcttaactt tcaactccgg
    25081 gtttttatac agctcaaaaa gttgtttctc gactttctgt actacctgcg ctcgtgcgcc
    25141 gcctttgtag tattcgccca tttcaatagc cagcatctct ttttgcttga agtaataaag
    25201 caaataagaa cacggcaaca gattcagaga acgaattaag ccttcgctaa atggcagatc
    25261 gaaaatattt ttaacgccag atgcttttaa ctgcccggat gccacaccat caagcaattc
    25321 ggcaaagcgc gacttgccat ttaccagcac atccttaatg aacaccatat ggttgaggcc
    25381 gaacaaatcg atagataaat catcgctgtc tttcagcatc agaacatcgt gaataaacat
    25441 cttcatgccg atcggaatat tacacacgcc gataaagcgt ttaaatccgg tatgacgata
    25501 aacggcttca gtaaccattc ccgccgggtt agtgaagtta atcacccatg cattcggaca
    25561 aagttcttcg acatctttta cgatgtcaaa aatcaccgga atggtacgca gacctttaaa
    25621 cagaccgccc gcgccgttgg tttcctggcc aagataacca tgacttaatg gaatacgttc
    25681 atccagttcg cgcgccggta attggcctac gcgcagttgg gtagtaacga aatcagcatc
    25741 tttcaatgct tcgcggcgat ccagcgtttt ataaagcttc atcgggacgc cagcgttatc
    25801 aatcatccgt tggcagagtt caaaaatgat atccagtttc tctttaccat cttcgacatc
    25861 caccagccat aattcgctga ccggcaattc gtgataacgc ttaataaatc cttccagtaa
    25921 ctccggggta tagctgctcc cgccaccaat agtgacgact tttaatttct ggctcataat
    25981 ttctcccttc agtacagaat actgatatct ggcatatctg cccccccgga cataaataat
    26041 ccagcaacag gacagatatg tgaattgtca ggtataacga cttactgatt aaattcagtc
    26101 aatttcttac gatagctctt aggcgtaaag gacgttaatt tcttaaacgt tttaataaac
    26161 aaactcgggc tactataacc ggcctcaaac gcaatatccg tcacggaata gttggtcatt
    26221 tccagttgtt ttttggcaaa attaatacgg atctcattaa taatctgcat tggtgttttg
    26281 ccataatatc gttgagtcgc tcgcgtcaaa tattcctgtg atttggctga caacgtcacc
    26341 atattctcca gcgccgattc actaaactgc tctttatcat gcatcttttc taccgtactt
    26401 ttcagccact gcggtacatc atcaatcacc tgttcttcgc ggtaatgacg taaacggtta
    26461 atgacataaa aagtaaccat ctcaacaaac tcttccagcc ctgtttcgcg gaaattcaat
    26521 gaagaaatca ctgtttccac ataggtgaga aaagcgttat tggtccggta tacctgcgaa
    26581 gcgacaaagc aataaggcaa caacggcagg taatgctgct caaaaaagcg tttactgatc
    26641 ccaacgttca atatacgcgt ggcaccaaac tcataaaaac tttgatggtg cgaacctaac
    26701 ggaataaaaa caaaatcgcc ccgttccagt aacacgcgct taccgttaat ctcctggaaa
    26761 taacgcccgg ttaataccag agtaaattca taatagtcgt gctgatgcag cccgctgata
    26821 ctctcagttt tgttatagat aaacacatgg aagtttttgc cattaaacaa ctgctgttct
    26881 cgggcagtgg caatttccgg cgcgttaatc actggctgca tcatcgactc cttatgcctt
    26941 cagtttttca tgaagctcaa ttaattcagt aatcagttca cgcgcaagca tggacgtcat
    27001 taaatgatcc tgagcgtgga ccagcaccag actcactttc atcttacctt cgcccgcatc
    27061 gccttcaatc agtttcgtct gtaccagatg cgcttcattc aatgccatcc gtgactgatc
    27121 catcatggct tttgctgcag caaaatcgcc ctgcttcgcc tgtttcagtg ccgcatacgc
    27181 caggctgcgc gcttgtccgg agttgatgat aagccccatc accacttctt ccagctcttc
    27241 agcttccgtt tgcgtatcgg gaatgttatc gagatccatc atacatcgtt cctctctatt
    27301 cttaccggca cgattacccg taccggcatc gattaaaatt tcagggcgtt agcgatatct
    27361 tcttcgcgct cttctttatc aatcgcgttt tgtgctttgt tagccaccac aacaaagggc
    27421 agataaatta acgttgcgat accaaggttg aagagtgcaa ccagcaatgc ggcgacgcta
    27481 ccgttggtgt taaagaaggc tcccagaccg gttggcatgg tccacggtgc aatattggtc
    27541 accggaggaa tgatgcccat gtagtacgct gcgagggtga ttgccgccag aatcggttgt
    27601 accagtacaa acgggataaa catcaccggg ttcatgataa ttggcagacc aaacagaatc
    27661 ggttcgttaa tctggaagat gccggacggc agcgccagtt ttgccacctg acgataatca
    27721 gcacggcgag aggcgataaa gatagccagg atcaggccta aagtcgcacc actgccccca
    27781 aggaaaataa aggagtccag catcggcttg gcccagatat ggaaggtctt accggctgcc
    27841 agcgccgctt cgacggaacc atattgctga taggtcgcga tattttccag cgcccacggc
    27901 gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc catgaatacc gaagaaccag
    27961 agcagtggaa caaagatcac ataggcccag cccaccacgc tacccaacga tgccagtggg
    28021 gttgagatgg tatccataat gatctgatgg aagttggtgc cccaggtatt caacgcccag
    28081 gcaataatcc ccatcacgga aagaataata aagccgggaa ttaatgccga gaaggaacgc
    28141 gatactgaag caggtacgct atcgggcaat ttaatgaccc aattgcggcg gacaataaag
    28201 gtaaacattt ctgccaccac caggccaata ataatcccgg agatgatatt cgctccacct
    28261 aaccagtttg cgccaaccac ataggcctca ccgacactat atggggtgac ggtcataaat
    28321 gctgcaacgg ataacaaccc agccgccagc gcatcgactt tacgctcttc tgccagcgcc
    28381 atgccaataa agaacggtgc cattaaagac attatcccta atgttccgtt atatacgttg
    28441 ccgccaatac ctttcaggcc attaagtgtt tcaatggttg aggcgtcgag gcgaatacct
    28501 aaggaataaa aaaacgcccc ctccccaaag cttagaaaaa cgttgttaat taatacaaac
    28561 atggcccccg caagggttaa cggcattaag cgaataaagc catttttgat tgcattaacg
    28621 tgtggctgct ttcctatttt aactgcaaaa gggaggagta ccttttcaag cgatgcaata
    28681 acattactca tagaaaaata cccttaaaaa ccgcaattta aatattgcgg tattgattta
    28741 tgaaataact ctttgacggg aaaatttaaa ataaattaat ttgctgcggc ttttttaatc
    28801 gctgcaaccg cagccttaag cacgcctaaa ccatcgactt tgccataaag cagcgagtca
    28861 attacttcaa ccggtttgtt gggtaacaaa cgctggattt cgggcaacat ataagcaatc
    28921 tgcggcccta ataacacgac atcggcattc tgaccttttt caccagccag tgtttccgga
    28981 aatgcttcaa taatgaccgg aacttcatat ttttctgcct gtgcgcgcat ttttgatacc
    29041 agtaaagagg tagacatgcc cgcagaacaa aacagataaa tgtgtttctt ttccataaaa
    29101 ctgccctcat cgacgattat ctgtcagcca gacactccgc aagccttaac ctgcttccat
    29161 gctctgggta acttgcgaaa ccaacatgac tttttttgtg gctgaaagga gtatacggta
    29221 tagacctgag caatagtatt tctttgacct tataaattgt ttctccttga cctgtggtta
    29281 tgaccctctt cacatttcgg gcaaataatt cgcgaagata attaagaact caggccaaaa
    29341 aaaaccggcg caatggccgg tttccgttgt tactcaagct ttcagacgaa ttgattactt
    29401 cgcagcctgt ggatcagtgt cgtattcagc acaggtctga taaccggagt tgatgacatg
    29461 tccggtatca tctaacgcga caaagtaggt ttctgcttta ccatcacgtt gacccaggat
    29521 gtaggtctgg caagtaccac gagcatggat catgctcact tcagacgaag gtttacccgc
    29581 aatttgtgca acctgcgccc ggctcatgcc ttttttgacg tctttcacca caggctgtac
    29641 aaactggtct ttagtacgat cataagccgt acaacccgcc agcatggtta ataccgccgc
    29701 tgcactcaga attcctgcca tattcttgtt catattccgt cctcttgttt atcagcgtgt
    29761 tagataagcc tggaatacat tgggcgcttt ttcaagcccg cgaacgaaac ggctccgctt
    29821 tcagaggatt cctgtatgac gttttaacca ccattcagcc cgctgtcgct tgtcgtttca
    29881 gtggcaacgg gttagcttta aggaagtttt gtcttttctg tctggagggg ttcaatgaca
    29941 ttgcaacaac aaataataaa ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa
    30001 attcgtcgta gtatcgattt tctgaaaagc tacctgcaaa cttatccgtt cattaaatca
    30061 ctggtgctcg ggatcagcgg cggtcaggac tccacgcttg ccggaaagct gtgccagatg
    30121 gcgattaatg agctgcgcct ggaaaccggc aacgaatcac tgcaatttat tgccgtacgc
    30181 ctgccctatg gtgttcaggc cgacgaacaa gattgccagg atgccattgc ctttattcaa
    30241 ccggatcgcg tattaaccgt taatatcaag ggcgcggtat tggccagcga acaggcattg
    30301 cgggaagcag acattgaact gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg
    30361 atgaaagcac aatatagcat tgcgggtatg accagcggtg tcgtggtggg caccgatcat
    30421 gcagcagaag ccattaccgg attcttcact aaatatggtg acggcggtac agacattaat
    30481 ccgctgtatc gtctcaacaa acgtcagggt aaacagttac tggcggcatt aggttgcccg
    30541 gaacaccttt ataagaaagc gccaacggcc gatctggaag atgatcgccc ttctctgcca
    30601 gatgaagtgg cactcggcgt gacctatgac aatatcgacg actatctgga agggaaaaac
    30661 gtacctgaac aggtcgccag aacaatagag aactggtatc tgaaaaccga acataaacgc
    30721 cgtccgccaa ttaccgtttt cgatgatttc tggaaaaagt aataatttgc acataatggt
    30781 ttgtgcgaaa aagagtaatt tgttcacgcc ggatgcggcg tgaacgcctt attcgaccta
    30841 caaaactatg caaattcaat atattgcagg agtcgaatag gcctgacagg cgtagcacgt
    30901 cagacggtgt aacctttgtc atcgacccgc ctctttttta atcgctcccc gcctgttaca
    30961 ctggatagat aaccagcatt cggagtcaac agtggtacgg cgtttaactt ctccgcggct
    31021 cgaatttgaa gctgcggcaa tttatgaata tcccgaacat ttacgttcat tccttaatga
    31081 cttacccacc cgaccagggg tgtatctgtt tcatggcgaa agtgacacca tgccgctcta
    31141 tatcggcaaa agcgttaata tccgcagtcg ggtgctttct catttacgca ccccggatga
    31201 agccgccatg ctacggcaat cccggcgaat tagctggatc tgtaccgcag gcgaaatcgg
    31261 cgctctgctc cttgaagcgc gattaatcaa agaacaacag ccgctgttta ataaacggtt
    31321 gcgccgcaat cgccagctct gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta
    31381 tgccaaagag gtggattttt cacgagcccc caacctgttt ggcctgtttg ccaataggcg
    31441 cgcagctttg caagcattgc agaccatcgc tgatgaacaa aaactttgtt atggcctgct
    31501 gggactggaa ccgttaagtc gcggtcgtgc atgttttcgt tcagcgctaa aacgttgcgc
    31561 cggagcatgc tgcggtaaag agagccatga ggaacatgcg ctacgcttgc gccagtctct
    31621 ggagcgtttg cgggtggtgt gttggccttg gcaaggtgcg gtggcactga aagaacagca
    31681 cccggaaatg actcaatatc atattattca aaactggctg tggctggggg cggttaattc
    31741 gctggaagaa gcgacaacgt taattcggac acccgccggg tttgatcacg acggttataa
    31801 aattctttgt aagccgctgc tttccggtaa ctatgaaatt actgaacttg atccggcgaa
    31861 tgaccagcga gccagttgat ttcactgaac aacagtttac cttgcggttg taaaagccgc
    31921 agggtgtgcc tgccatcgaa ccagcacgcg ccctggtcgg tagtgagcaa tttatcgccc
    31981 agttgccacg ccccgttaat cacaaacacc accccgccgc gtgagccaaa ggtagtaaac
    32041 gtacgttcgg caattctgac tttcgcctta cagacatcca ggcgtgtcat gatgttgaaa
    32101 tccatcgaca tctgcccttc cgtcagtttc gcttttacca cctggtccgc tgcaaaggca
    32161 aaaggctgta acggttttaa ggtatggtta aagcggtctg cgctttcaag gagcatctcg
    32221 ccgccttcca gcaacgtcac tatcctttcc atgccgggaa acaaagaaaa ctccccattc
    32281 gccgcgatgg atgcaatgct ggcacgccag taaaaatcac gttttgccgg tgggaaagtg
    32341 caaatttcgc gcgtttcacc ggcagcattt cgccacagat tcaccgacat tttacgcata
    32401 tcaaagtatt ccatactcgc tcccggcccg ttcttttcat tgttatcgtt caggtaaccg
    32461 tggatggtta tcggcaactc gggtgattta cgttagtggt gatcaggctt tttttacaac
    32521 tgtttgagaa gagaaaagaa aaccgccgat cctgtccacc gcattactgc aaggtagtgg
    32581 acaagaccgg cggtcttaag ttttttggct gaaagattat tcagcagttg caggcatttt
    32641 accttttg
//
LOCUS       DAZRVY010000037        30872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZRVY010000037 DAZRVY010000000
VERSION     DAZRVY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.037"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            472..987
                     /locus_tag="ACHXOX_001755"
     CDS             472..987
                     /locus_tag="ACHXOX_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7005241.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1205..1933
                     /gene="artP"
                     /locus_tag="ACHXOX_001756"
     CDS             1205..1933
                     /gene="artP"
                     /locus_tag="ACHXOX_001756"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR7005242.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1951..2682
                     /gene="artI"
                     /locus_tag="ACHXOX_001757"
     CDS             1951..2682
                     /gene="artI"
                     /locus_tag="ACHXOX_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR7005243.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTSLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2689..3405
                     /gene="artQ"
                     /locus_tag="ACHXOX_001758"
     CDS             2689..3405
                     /gene="artQ"
                     /locus_tag="ACHXOX_001758"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR7005244.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3405..4073
                     /gene="artM"
                     /locus_tag="ACHXOX_001759"
     CDS             3405..4073
                     /gene="artM"
                     /locus_tag="ACHXOX_001759"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR7005245.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4365..5096
                     /gene="artJ"
                     /locus_tag="ACHXOX_001760"
     CDS             4365..5096
                     /gene="artJ"
                     /locus_tag="ACHXOX_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR7005246.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5271..6398)
                     /gene="rlmC"
                     /locus_tag="ACHXOX_001761"
     CDS             complement(5271..6398)
                     /gene="rlmC"
                     /locus_tag="ACHXOX_001761"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR7005247.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6439..6927)
                     /locus_tag="ACHXOX_001762"
     CDS             complement(6439..6927)
                     /locus_tag="ACHXOX_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR7005248.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6987..7832)
                     /gene="potI"
                     /locus_tag="ACHXOX_001763"
     CDS             complement(6987..7832)
                     /gene="potI"
                     /locus_tag="ACHXOX_001763"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR7005249.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7829..8782)
                     /gene="potH"
                     /locus_tag="ACHXOX_001764"
     CDS             complement(7829..8782)
                     /gene="potH"
                     /locus_tag="ACHXOX_001764"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR7005250.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8792..9925)
                     /gene="potG"
                     /locus_tag="ACHXOX_001765"
     CDS             complement(8792..9925)
                     /gene="potG"
                     /locus_tag="ACHXOX_001765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR7005251.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10020..11132)
                     /gene="potF"
                     /locus_tag="ACHXOX_001766"
     CDS             complement(10020..11132)
                     /gene="potF"
                     /locus_tag="ACHXOX_001766"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR7005252.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11483..11959)
                     /locus_tag="ACHXOX_001767"
     CDS             complement(11483..11959)
                     /locus_tag="ACHXOX_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR7005253.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12047..12949)
                     /gene="rimK"
                     /locus_tag="ACHXOX_001768"
     CDS             complement(12047..12949)
                     /gene="rimK"
                     /locus_tag="ACHXOX_001768"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR7005254.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13010..13732)
                     /gene="nfsA"
                     /locus_tag="ACHXOX_001769"
     CDS             complement(13010..13732)
                     /gene="nfsA"
                     /locus_tag="ACHXOX_001769"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR7005255.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13716..14003)
                     /locus_tag="ACHXOX_001770"
     CDS             complement(13716..14003)
                     /locus_tag="ACHXOX_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR7005256.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14163..14420
                     /gene="grxA"
                     /locus_tag="ACHXOX_001771"
     CDS             14163..14420
                     /gene="grxA"
                     /locus_tag="ACHXOX_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR7005257.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14450..14827)
                     /locus_tag="ACHXOX_001772"
     CDS             complement(14450..14827)
                     /locus_tag="ACHXOX_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR7005258.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15097..16782
                     /locus_tag="ACHXOX_001773"
     CDS             15097..16782
                     /locus_tag="ACHXOX_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR7005259.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16957..17493)
                     /gene="rcdA"
                     /locus_tag="ACHXOX_001774"
     CDS             complement(16957..17493)
                     /gene="rcdA"
                     /locus_tag="ACHXOX_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR7005260.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17577..18785
                     /locus_tag="ACHXOX_001775"
     CDS             17577..18785
                     /locus_tag="ACHXOX_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005261.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18785..19600
                     /gene="ybjI"
                     /locus_tag="ACHXOX_001776"
     CDS             18785..19600
                     /gene="ybjI"
                     /locus_tag="ACHXOX_001776"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR7005262.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19686..19970
                     /gene="ybjH"
                     /locus_tag="ACHXOX_001777"
     CDS             19686..19970
                     /gene="ybjH"
                     /locus_tag="ACHXOX_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR7005263.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20011..21238)
                     /gene="mdfA"
                     /locus_tag="ACHXOX_001778"
                     /pseudo
     CDS             complement(20011..21238)
                     /gene="mdfA"
                     /locus_tag="ACHXOX_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21523..22119
                     /gene="ybjG"
                     /locus_tag="ACHXOX_001779"
     CDS             21523..22119
                     /gene="ybjG"
                     /locus_tag="ACHXOX_001779"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR7005264.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22177..22935
                     /gene="deoR"
                     /locus_tag="ACHXOX_001780"
     CDS             22177..22935
                     /gene="deoR"
                     /locus_tag="ACHXOX_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR7005265.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22982..24184)
                     /gene="dacC"
                     /locus_tag="ACHXOX_001781"
     CDS             complement(22982..24184)
                     /gene="dacC"
                     /locus_tag="ACHXOX_001781"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR7005266.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24431..25056
                     /gene="gstB"
                     /locus_tag="ACHXOX_001782"
                     /pseudo
     CDS             24431..25056
                     /gene="gstB"
                     /locus_tag="ACHXOX_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25053..26168)
                     /gene="yliI"
                     /locus_tag="ACHXOX_001783"
     CDS             complement(25053..26168)
                     /gene="yliI"
                     /locus_tag="ACHXOX_001783"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR7005267.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26279..26662)
                     /gene="bssR"
                     /locus_tag="ACHXOX_001784"
     CDS             complement(26279..26662)
                     /gene="bssR"
                     /locus_tag="ACHXOX_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR7005268.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26875..28200
                     /gene="rimO"
                     /locus_tag="ACHXOX_001785"
     CDS             26875..28200
                     /gene="rimO"
                     /locus_tag="ACHXOX_001785"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR7005269.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28247..29568)
                     /locus_tag="ACHXOX_001786"
                     /pseudo
     CDS             complement(28247..29568)
                     /locus_tag="ACHXOX_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29576..>30872)
                     /locus_tag="ACHXOX_001787"
     CDS             complement(29576..>30872)
                     /locus_tag="ACHXOX_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR7005270.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7782 a         7963 c         7479 g         7648 t
ORIGIN      
        1 tatcgagagt caaagctggg actatcaaat ttcgctcatt ttcaaaaata caacgtaatt
       61 ttggcaatca tcttaatcgc aggatctctt atcgcatgtg aaaaattttg taatgattag
      121 acaataaccg cgttcattta tcacgcgtta taaatgtgaa actggcttct tgcagatgct
      181 cttaacctct acaggaggat ttataccacc atttcagaac tatcatattt accttaaatg
      241 atcatttcag ccatataaca cgctcaacct cacaaatggt ttacactgtc tattgtttgg
      301 gtaatttaag taagtcagct acgagcaacc ttgagtggat agccagcaat ggactcaaac
      361 ccaatcaggt gttcagattg tttcagaatt gtattaaaaa caatgtatta aataatctga
      421 tagcgtttgg tcgtagtgtg acgcagacag caagctaata aggaaatgat tatgcgctac
      481 cgttcactat ctctacttat tccatgtgca ctgctgctga gtgcctgtac caccgtcacg
      541 ccagcttata aagataacgg tccccgcacc ggttcctgtg tccagggggg gcctgacagc
      601 gtggctcaac aattctatga ctaccgcatt caacatcgca gcaatgacat taccgccctg
      661 cgcccctatt tgagtgacaa actggcaacc cttcttagcg atgccagccg cgataatagt
      721 catcgtgaat tgttaagcag cgatccgttc tccagtcgaa ccaccttgcc agatagcgcc
      781 cacgttgcca gtgcatcgac tatccctaat cgtgatgccc gtaacattcc attgcgcgta
      841 gatctcaaac agggtgacca gggttggcag gatgaagtgt taatgattca ggaaggccag
      901 tgttgggtca tcgacgatgt gcgctactta ggcggcagcg ttcatgccac ggcgggtacg
      961 ctccgtcagt ccatcgagaa tcgctaacgc cttgatcaac caataaaccc cgtaaattgt
     1021 ccggcatttc gtgaggaatg ccgacattta tgctcgccga ccaccgcccc cgttattttg
     1081 tgctatgttt attgaataat gcgctttgct tttaactttt aaagcagaaa tattgcataa
     1141 ttattctgtc aaaggtacta tcttcgacct caatagctat cagactgcca gtatacgagt
     1201 gtcaatgagt attcaattaa acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt
     1261 cgatatcacg ctggattgcc cacagggcga aacgctggtg ttacttggcc ccagcggcgc
     1321 gggtaaaagc tcgctgctgc gtgtactcaa tctgcttgag atgccacgct ccggtacgct
     1381 caacattgca ggcaaccatt tcgatttcac caaaacaccc tctgacaaag cgattcgcga
     1441 tttgcgtcgt aacgttggca tggtgtttca gcaatacaac ctgtggccgc atctgaccgt
     1501 gcagcaaaac ctgattgaag cgccctgccg tgtactgggg ttgagtaaag atcaggcgct
     1561 ggcccgtgca gaaaaactgc tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc
     1621 gctgcatctt tctggtggtc agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga
     1681 accgcaggta ctgctgttcg atgaaccgac cgccgcactg gacccggaaa ttacggcaca
     1741 aatcgtcagc atcattcgtg agctggcaga aacgaatatt acccaggtga tcgtcaccca
     1801 cgaagttgaa gtggcgcgta aaaccgccag ccgagtggtg tatatggaaa atggtcatat
     1861 cgtagaacaa ggcgacgcga gctgctttac cgagccgcaa accgaagcat ttaaaaacta
     1921 tctctctcac taagattcgg gataacaaca atgaaaaaag ttctgattgc cgcgttaatt
     1981 gcaggtttta gtctttccgc cacagctgcc gaaaccattc gttttgctac cgaagcatcc
     2041 tatcctccgt ttgaatcgat tgatgcaaac aaccagatcg ttggttttga cgtcgacctg
     2101 gcacaagcgc tgtgtaaaga gattgatgca acctgcactt tctctaacca ggcgtttgac
     2161 agcctgatcc caagcctgaa attccgtcgc gtagaagccg tgatggcggg catggatatc
     2221 actccggagc gtgaaaagca ggtgctgttt accaccccgt actatgacaa ctctgccctg
     2281 tttgtgggtc agcaaggcaa atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc
     2341 gtacagaacg ggacgacaca ccagaaattc attatggata agcacccgga aatcactacc
     2401 gttccgtatg acagctacca gaacgcaaaa ctggatctgc aaaacgggcg tatcgacggc
     2461 gtcttcggtg acaccgcagt ggtcactgag tggctgaaag ataacccgaa actggcggcg
     2521 gtgggcgaca aagtgaccga taaagattac ttcggcacta gcctcggcat cgcggtacgt
     2581 cagggcaaca ctgagctgca gcagaaactc aacactgcgc tggaaaaagt gaagaaagat
     2641 ggcacttacg aaaccatcta caacaaatgg ttccagaagt aattcctgat gaatgaattt
     2701 tttcctttag caagcgccgc cgggatgacc gtcggccttg ccgtttgtgc attgattgtc
     2761 gggctggcgc tggcgatgtt ctttgcggta tgggagtcgg caaaatggcg tcctgtcgcg
     2821 tgggcaggtt cagcgctggt aaccattctg cgtggcctgc cagaaattct ggtggtgctg
     2881 tttatctatt ttggctcctc gcagctgctg ctgacgcttt cggatggctt cactatcaat
     2941 cttgggttcg tgcagatccc agtgcagatg gacattgaga acttcgacgt tagcccgttc
     3001 ctttgtggtg tcatcgctct gtcactgctg tatgccgcct atgcctcgca aacgcttcgg
     3061 ggcgcgttga aagcggtgcc ggtgggtcag tgggagtctg gtcaggcgct ggggctgtcg
     3121 aaatcggcta tctttttccg tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggc
     3181 ctcggtaacc agtggctggt gctgctgaaa gataccgcgc tggtcagttt gattagtgtg
     3241 aatgatttaa tgctgcaaac aaaaagcatc gctactcgta cccaggaacc atttacctgg
     3301 tacattgtgg cggcggcgat ttacctggtg atcaccctgc tcagtcagta cattctcaaa
     3361 cgcattgacc tgcgcgcgac acgttttgag cggaggccca gctaatgttt gagtatttac
     3421 ccgaactgat gaaagggcta cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg
     3481 cactgattct ggcattgatt tttaccatca tcctgacgct gaaaacgccg gtgctggtgt
     3541 ggctggtgcg gggttatatc acgctgttta ccggtacgcc gctgctggtg cagatcttcc
     3601 tgatttatta cgggccgggc cagtttccga cgttgcagga gtatccggca ctgtggcatt
     3661 tgttgtcaga accgtggtta tgtgcgctga ttgcgttatc gctgaacagt gcggcgtata
     3721 ccacgcagct gttttacggt gcaattcgtg cgatcccaga aggtcagtgg cagtcctgta
     3781 gcgccctggg aatgagcaaa aaagatacgc tggcgctcct gctgccgtat gcctttaaac
     3841 gctcgctctc ttcttattcc aacgaagtgg tgctggtgtt caaaagtacc tctctggctt
     3901 acaccattac gctgatggaa gtgatgggat acagccagtt gttgtacgga cgcacctacg
     3961 atgtaatggt gttcggtgcg gcagggatta tttacctggt cgtcaacggc ctgctgacgc
     4021 tgatgatgcg tctgatcgag cgcaaagcac tggcattcga acggcggaat taatgtagct
     4081 gcgtacattc gccggggggg tttgtaggtc tgataagacg cgccagcgtc gcatcagaca
     4141 tcagcacggt gcctgtgccg gatgcggcgt ggacgcctta tccggcctac atagtgcata
     4201 aattcttata atgaagacgg acaactcact aagttatccg tcttttttta tttcatttaa
     4261 attatttaat catatttatt gcatataaat tcacttgatg gcattgttat cccatgccgc
     4321 agacacggcc aaaaatcata agattgacag acgggagttc catcatgaaa aagttagttc
     4381 ttgccgcttt acttgcttcc tttactttcg gtgcttctgc cgcagagaaa atcaattttg
     4441 gcgtttcagc cacctatcca ccctttgaat ctataggtgc taataatgag attgtcggct
     4501 ttgatatcga tctggcaaaa gccttgtgca aacaaatgca ggcagaatgt acttttacta
     4561 atcacgcgtt cgacagcctg atcccgtcac tgaaattcag aaaatatgac tccgtaatct
     4621 ccggtatgga tatcacgccc gagcgtagca agcaggtatc gtttaccacg ccctactatg
     4681 aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa aacgtttgcc gatctgaaag
     4741 gcaaacgtat tgggatggaa aacggcacca cgcaccagaa atatattcag gatcagcacc
     4801 ccgaagtgaa aactgtctcc tatgacagtt atcagaatgc ctttatcgat ctgaaaaatg
     4861 gtcgtattga tggggtattt ggtgacacag cggtggtaaa cgaatggctg aaaaccaatc
     4921 cgcaactggg cgttgctact gaaaaagtga ccgatccgca atacttcggc accggtctgg
     4981 gcatcgctgt acgtccggat aacaaagctc tgctggaaaa actgaataac gcgctggcag
     5041 caattaaagc tgacggtaca tatcagaaaa tcaatgacca gtggttcccg cagtaattga
     5101 gactgatgac aaaagtaaga ttgcctgatg cgctgcgctt atctggccca catcaactct
     5161 gcaatgtatt gatttagcat gattttgtag gccggataag gcgtacacgc cgcatccggc
     5221 atgaacaaag cgcacctgat cagcaatctg aagccgcacc tgcggctttt ttattgcttc
     5281 accagcagcg tcatcacttc atagtgcgcg gtgtgcggga acatatcaaa aagctgtacc
     5341 cgttcgatgc ggtaacctgg cagttcgcgg atatctttcg ccatggtttg ggcgttacag
     5401 ctggagtaga taataaaacg cggtgccatc gttgagagat aatcacacag cggtttacca
     5461 atgccgcggc gcggcgggtt aaccagcacc agctccggca catccccctg agcggtggca
     5521 aactgagtgg agtccagcgc ctgaaattgc aaacgcgtta agcccagttc agcggctgac
     5581 tgctttgcac aggcaatggc ctctgatgca atttcgatcc cggttaactg catgtcaggc
     5641 gtcgcgcagt gtaaaccaaa gccacccaca ccgcagaaca gatcccacat atgtttaact
     5701 ggcagctgcc gtacccagtc gcgcgcggta gcgtacagct ggctggcgac cgccggatta
     5761 gtctggaaga aactttgcgg acggatccac agcggtacgt cattaaaacg ctctgccagc
     5821 gcctgttgtt cagtcaggta gatctccgtc tccccttcca taatcgccat atgtaccggc
     5881 tgaatattga cggtaataac tttcagttgc ggtagttgtt cctgtaacca cggcagcgcc
     5941 ttacgcagtt gtgccagttt ggtttcagaa cgcagtacaa agcgcagcat catgccgcca
     6001 tcgctctggc tttcagtcag cagaatgtat ttcagttcgc cacgcttacg cgccacgttg
     6061 tagggcgtta accccgcacg ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaaa
     6121 gaggcaggat aaagcgggca gtcacaaagg tcttccggcg tgccatcgcg atgcagcata
     6181 ccgagcagtg gtttttcaac gctaccactc accaccattt tggctttatt acgaaaccct
     6241 tgttccgggc ctgacaccgg cgcgcaccat tcctcaaccg gaaaatcggc gagcagattt
     6301 ttaagatcgg cggttttagc ggagagttgc tctggaatcg gctgcgttat ccactgacag
     6361 gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac attgaccttc acatcatcag
     6421 gggcgacgat tatacacatt attgcaactg gaagaaccgc cgactggtcg agggaacgaa
     6481 cagcagcatc aggatgagca tatccggcag cttctgcagc atcaggctat ggaagatttc
     6541 acgttttgat tcaccgggaa tgctgaacag ctccggataa ccgtacccca gcgaagccgc
     6601 ccacaagtaa cttgcggcgg tgatttgtgt cagcagatat agccagcgcg cccagcgacg
     6661 ccctttcacc agtgaaaacg cacaccagat ctcaatgaaa accagcacca gactgcttaa
     6721 aaagaccagc gttaaactcc aggtttgtac gctgcgatga atgaactcgc caataccgcg
     6781 cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca cggatcataa taatggcgag
     6841 cgccgccacc tgcaccagcg caggcacatt caggcgagca tgagatgaag atgttttctt
     6901 aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg cggcatgcgc ggcattatgt
     6961 agccaggttg gcaaatttta gtgtcttcag ccatgtcttg cacgctggat atcgcgtatc
     7021 cgctgttttt ctgcgcgagc catcagatac caggcgataa atccgacaat tccgaccgca
     7081 ccgagaatta acgttgcaag ggcgttgatt tccggattca ctcccatccg cacgctggaa
     7141 aagaccagca tcggtaacgt ggtggctccc ggcccggaaa caaagctggc gatcaccaga
     7201 tcatcaagcg acaaagtaaa agccagtaac cagccagaaa tgatcgcggg catgatcatc
     7261 ggtagcgtaa tgacgaaaaa cactttcagc ggcgtcgcac cgagatccat cgctgcttct
     7321 tctatcgagc gatccagttc ccgcaatctt gaagaaataa cgaccgccac ataagccgta
     7381 caaaaagtga catgcgccag ccagatggtg agcataccgc ggtccgcagg ccagccaatg
     7441 gcatgagcaa gcgcgacgaa taataacaac agcgacaagc ccgtgatgac atctggcatc
     7501 accagcggcg cggtgatcat aaaggcaaaa ccatttgatc cgcgaaacct gccaaaacgc
     7561 accagcacca ccgccgcaat agtcccgagg atcgccgccg ccgttgccgc acaggcagca
     7621 attgtcaggc ttaaaccaac cgcactcatc atcgcgtcat cgcgcaataa ctcaccatac
     7681 cagcgcgttg accagccggc ccacaccgtc accagcttcg agctgttaaa cgaatagata
     7741 accagcatca gcattggcgc gtagagaaag gtaaagccca gcagcaaaat cacaatccgc
     7801 cagggcgaac gaactaccgg taaattattc atccgtgttc tcccacgctt ttttgctggt
     7861 gtttgtggaa ccacattatc ggcacaatta gcagcagcaa catgatgatc gctaccgccg
     7921 aggccaccgg ccagtcgcgg ttattgaaaa actcttgcca taacacgcgc ccgatcatga
     7981 tgctgtccgg gccaccgagc agttccggga tcacaaactc gcccacagcc gggataaaca
     8041 ccagcatcga tccggcaata atcccacctt tagtcagcgg cacgatcaca gtaaagaacg
     8101 ttttcagcgg tcgtgcaccg agatccagcg ctgcttccac cagcgaataa tcaatacgaa
     8161 tcaacgcggt ataaatcggc agtaccataa acggcacgta agcgtaaaca atgccgatat
     8221 aaacggccag attggtatgc agaatggtca gcggttgatc gataaccccc agccacagca
     8281 gaaaattatt cagcacaccg ttgtttttta atattcccat ccaggcataa acgcggatca
     8341 gaaacgaggt ccacgacggc aggatcacca gtagtaataa aatattacgg gtcgaaggct
     8401 tactgtgcgc caccgcccac gccagcggat agccgatcag taaacagcaa aatgtcgaaa
     8461 tcgccgccac ctgtaacgac tggagataag catcaaaata gagcggatcg tcggtcagtt
     8521 gcagaaaatt accgagatta agagtgatgg aaagttgccc gtcagcccac tccatcagtt
     8581 cggtataagg tggaatagcg cgcgccatct ctcccaggct tattttaaag acaatcagaa
     8641 atggcagcag aaacagcaag atcaaccaga tatatagcaa cgcaatgacc agtttgcgcc
     8701 catgcttcat ttgcagctgc gacaaccaca gcttaaatcc gcccggcggt ttcgactggg
     8761 cagcaggttc aagtgtactc atctttgctc cttaaaccgt cagcaccaca cagctgtcca
     8821 cttcccagca caaacgcact tcgtcgcccc aggtcggtaa ccctttacga tgacgatggg
     8881 cgttttgtag ctgggcgctg atcatctgcc cacttttcag tcgaacgtga tacaccgaaa
     8941 gatcgccgag ataggcaatg tgtatcacct cccccaccgc gaagttacaa ccattggcgg
     9001 gcggctcttc gcaaagcatg attttttccg ggcgcagcgc tacgtgtacc ggcacgttat
     9061 cgaccactga agcatccgca tcgactttca gtggatgcac cagccccggc gaatcaagca
     9121 ccaggccatc ttcctgacgc tctttgagta caccttcaaa gacattaacc gaaccgataa
     9181 attcagcgct gtagcgggta gttggatgct cgtagatctc ttccggctcg ccaatctgga
     9241 caaatttccc gcgattcata atggcgatac gccccgccat ggtcatcgcc tcttcctgat
     9301 cgtgggtgac catcacacaa gtcacaccga cgcgctccag aatatccacc acttcaagct
     9361 gcatccggtc acgcagcttt ttatccagcg cgcccatcgg ctcatcgagc agtaatagtt
     9421 tcgggcgttt ggcaaggctt cgggccaggg ccacacgctg tcgctgacca ccggaaagct
     9481 gatgcggttt gcgtttggcg aactcctgca tgtgcaccag gccgagcatc tcatggaccc
     9541 gactggcaat ttccgctttc ggtagtttgt cctgtttcag gccaaaagcg atgttctgtt
     9601 ccacggtcat atgcggaaac agcgcgtaag actgaaacat catattgatg gggcgcaggt
     9661 aaggcggcac ctgtgacaaa tcgacgccat cgagcattat ctgcccggca gaaggttgtt
     9721 caaaacctgc cagcatacgc agcagcgtgg acttgccaca gccggatgcg cccagcagcg
     9781 cgaagatttc acctttgtag atggtcaggc tgacatcatc gaccgcatgc tgaccatcgt
     9841 aggatttggt gaggttgcgg atttctaata gcggcgtcag cgccttacgg gttttcgcct
     9901 gcgggcgagg gatagcgtca ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac
     9961 catgatggtg cgaattgccg gctggtgtgg tgcgcccgcc cggcatctaa ggctgcggat
    10021 tattttccgc tcttcacttt ggtccacgcg cgggtgcgca cacggtcgat tttcggatcc
    10081 tgtactttca gagtgaatag cttcgcacga acgtccgccg gaggataaat acccgggttc
    10141 tcacggactt ccgcactcac cagcggcgtg gctgctttat tggcgttggc atagaacaca
    10201 tggtcggaaa tatgcgctac tacatccggg cgcagcaggt aattcaggaa ctgataggct
    10261 tcgtctttgt ttttggcatc cgcaggcatg gcgaatacat caaagaacgc catcgcccct
    10321 tcttttggaa tcgagaacga gacattcact ccattcttcg cttccttcgc gcggtttgac
    10381 gcctgccaga catcacctgc ccagccgata gcgacgcaaa tatcgccgtt tgccaggtcg
    10441 ttaatgtatt gagatgaatg gaaataacga atgttcgggc gcagctttaa cagcagatcg
    10501 gttgccggtc cggtgtaatc atccgcttta gtgctgttgg gatctttgcc gagataattc
    10561 aacacggtag caaaaacttc ttctggcgca tccaggaaag agacaccgca gcttttcagt
    10621 ttttccagat tttcaggttt gaggatcaag tcccagctat cgacgggcgc gttttcgccc
    10681 agcacagctt taactttatc aacgttatag ccaatcccgg tcgtcgccca catatagggc
    10741 atagcaaatt tattgtcggg atcgtgtttg gcgaccagct tcagcagctc cgggtcgaga
    10801 ttcttccact ccggcaattt gcttttgtcc agcggctgga aaactcccgc agtcaactgg
    10861 cgttcaagaa agctggcaga tggaaccacc aagtcaaagc cggtgctacc cgccattaat
    10921 ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga cgactttaat accggtttct
    10981 ttttcaaaat tggccaccgt gtccggggcg atataatcag accagttata aatgtggagt
    11041 gttttttgtt cagccgcgag cgtgccgaca gagacggcca tcagagcacc cgcagccaga
    11101 cccgaaagcc attttttatt taaggcggtc atatctcttt ccttctgaaa gttcgttaac
    11161 aaacgaacaa aagttgtcac actaaaagat atgcaataaa cgtgcatatt ttttatcggc
    11221 ggggtgtaag aaaagagaac tctctcccgg ccagcactgc tcgctatata aaagcatcgc
    11281 aagaataact ttagccaggg tttgagacta tagctcggat taaaaaacgg tatttaacaa
    11341 atttttatgc gtatttgctc tatgtgataa agccaaatcg acacaagaga agtgaaataa
    11401 tctttaagaa aaggttaaaa acagcagata attttgcagc gcgcagccac tgcaccagcg
    11461 actgcgtgct cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc
    11521 cttcatcgtc agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa
    11581 tctgctcttc gctctgacgc acgaaccatg caaactgctc ataggttacg ccctgcataa
    11641 cggataaaga ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg
    11701 ctttcacggt cagcgaactg gcattgatgg cagataaatc cgccgccagc ggcaatacgg
    11761 ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt
    11821 tggcatcaaa gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat
    11881 cacattcaaa aaaactcatc cccaggtcat caagccattg acgcagcgta tccagaccag
    11941 gaacgaccag cgatgtcata attttgtaca acctctttcg ataaaaagac cggcacagct
    12001 tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt
    12061 tcaggcaata ttctgtcgta gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt
    12121 cgataccagt ggttttttct attccttcca gccccggcga cgcattcact tccatcacca
    12181 acggcccgcg attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg
    12241 cggcttttat cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc
    12301 cacgatgcaa attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca
    12361 cttcatcgcc aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct
    12421 gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctgac
    12481 gcgtctccgc cagcacgacg ccaattccct gcgtgccttc aaccaacttg accaccagcg
    12541 gcgcaccacc gaccatgtcg attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg
    12601 tgacaggcag gtcgatgccc tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg
    12661 cccgggcaat ggcgaccgac tcattgagcg gatagctccc cagcatctcg aactggcgca
    12721 gtgccgccgt cccataaaag gtaatggcgg tgccaatacg cgggatcact gcgtcaaaat
    12781 ggggtaactt gcggcctttg tagtgaatag aagacgccgc aggatttatg ttcatgtagc
    12841 aagaaagcgg atcaagaatt tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc
    12901 gcttacacga atagagcgtt ccatcccggg acaatatggc aattttcacc ctgcacctct
    12961 ctgtcagacc tggtcaaaag cccgcgtatc atacatcgac gtggcggttt tagcgcgtcg
    13021 cccaaccctg tttgtgcaaa taatccagaa taaatgggcg gctttcttta atgattgttc
    13081 ggcggatatg atcgctccag gtatcccggc gattattgct gccacgggtg aggtaatatt
    13141 ccgccagttg ctcgtcatac tgcgccagtg cgtctttatc cagcggttga tagctgtttt
    13201 catgcaccaa aatggaggcc ggtaaacgcg gcttgagatc cggattatcc gcaggccagc
    13261 caaggcacag cccaaacagc ggcagaacat gctgcggtaa tttcagcagt ttcgtcaccg
    13321 cttcaatatt attgcgcagg ccgccgatat ataccccgcc caatcccagc gattccgctg
    13381 cggttaatgc attctgcgcc atcattgccg tatcaacgac accgagcaac agttgttccg
    13441 ccaggccgag ctgagcatcc ggacagatct gtaaatggcg gttaaagtcg gcacagaaca
    13501 cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc ggtcagcgtc accagttctt
    13561 cacgtaatgc tttgtcggta atgcgaataa tgctactaca ctgcaaaaaa ctggaactgg
    13621 acgtcgcacg ggcgctgtta ataatcgcct cacactgcgc ttcggaaatg ggttcatcag
    13681 tgaaatggcg aatggagcga tggccacaaa taagttcaat ggttggcgtc attatctttt
    13741 tctctttctg aacgtgaata ttgcggtgga cggttcatca gctgtggggc aagacgtttt
    13801 gccacctgaa gaataaccac caccgcagcg ggaagcatga gcaaaacacc gagaaaaatc
    13861 atcagaatct gcacttctgg ccgagaaaat ggctcaggca gcgacaggga gtcgcttacc
    13921 gacagcagcg ccaccgccag tagcatcatt ccgataaatt ccagtatcaa cacgccttta
    13981 ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc agtctaaaac
    14041 ttttcactgt attgtgttta acagttatag cttttagcaa ttaatgcaac aggttaaacc
    14101 tactttcagc gaatacattt tagcgtgatc attacaggca taaatctatg aggagagaaa
    14161 taatgcaaac cgttattttt ggtcgttcgg gttgccctta ctgtgtgcgt gcaaaagatc
    14221 tggctgagaa attgagcaat gaacgcgatg attttcagta tcagtatgta gatattcgtg
    14281 cggaagggat cactaaagaa gatctacaac aaaaggcagg taaacccgta gaaaccgtgc
    14341 cgcagatttt tgtcgatcag caacatatcg gcggctatac cgattttgct gcatgggtga
    14401 aagaaaatct ggacgcctga tcgtctgaca agccctcgcg ttgagggctt tactgatttt
    14461 ttctgtgctg tggtttaaac aaactactga taaataagaa acacagtgcc cccagcgcac
    14521 accagaacac cgcgcttagt aaccatgcca gctcttgcca gaatgagcgc gtcggtgaaa
    14581 aaaacagccg cataatgagc attgaacagg gtgccgccag cattgcgcca aatagaggtt
    14641 tcaggacttc tctacgctgt gaaaagaaac tggcaactgc tccaggaaga atgaaaaaca
    14701 gcaagccgat ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc gtcgccagaa
    14761 aaaggcacac cacaatgaag aggacaaaac agcagattgc ccccgcccaa cgttgtttat
    14821 gtttcactcg ttcctcctga cactgcgtct atcgaacaca tttttcgcca gtgtggcgtt
    14881 cagtaagata aagccgcttc gcattccatg ctaatatagg ccaacgcaat tcatatagcc
    14941 gttgatacct aatgtgatta cactagtaaa atatattgtt actttactat cgtttaggtg
    15001 cgctgaatga atctgcgccc tgaattctgg taaaaaacat tatcgtaaat taccatttct
    15061 ttcaacagct tactagtaaa caagaagtta gcctccgtga atataaacgt cgccgaattg
    15121 ttaaatggga attacattct gttattattt gtggtcctcg cgcttgggct atgtctcgga
    15181 aagttacgac ttggttcgat ccaactgggt aattccattg gcgttttagt cgtatcgctg
    15241 ttattaggcc aacaacattt cagcattaac accgatgcgc ttaatcttgg ctttatgctg
    15301 tttattttct gcgtcggggt cgaagccgga ccgaactttt tttccatttt ttttcgcgat
    15361 gggaaaaatt acctaatgtt agcactggtg atggttggca gtgcgctggt gatcgcctta
    15421 gggttaggta agctgtttgg ctgggatatt ggcctgacgg ccggtatgtt agcaggctct
    15481 atgacgtcga caccggttct ggtcggtgct ggcgatacac tgcgtcattc cggcatggaa
    15541 agcaggcagc tctcactggc actggataat ctgagcctcg ggtatgcctt aacctattta
    15601 atcggtctgg tgagtttgat tgttggtgcg cgttacttgc cgaaattgca gcatcaggac
    15661 ttacagacca gcgcccagca aatcgcccgc gaacgtggcc tggacactga tgccaaccgt
    15721 aaggtttatt taccggtgat ccgcgcctat cgcgtcggcc cggagctggt ggcctggacc
    15781 gacggcaaaa atctgcgtga actgggtatt tatcgacaaa ccggctgcta cattgaacgt
    15841 attcgacgta acgggattct ggcaaatcca gacggtgatg ccgtgctaca aatgggcgat
    15901 gaaatagcgt tggtaggcta tcccgacgcc catgcccgac tcgatcccag cttccgtaac
    15961 ggtaaagaag ttttcgatcg tgaccttctc gacatgcgta tcgtcactga agaagtggtc
    16021 gttaaaaacc ataacgctgt aggtaaacgt ctcgcacaac tgaagttgac cgatcacggt
    16081 tgcttcctta accgcgtcat tcgtagccag attgagatgc cgatagatga caacgtcgtg
    16141 cttaacaaag gtgacgtttt acaagtcagc ggtgatgccc gtcgcgtaaa aaccatcgcc
    16201 gatcgcatcg gctttatctc gattcacagc caggtcactg acctgctggc attttgcgcc
    16261 ttctttgtta ttgggctgat gatcgggatg atcaccttcc agttcagcac attcagtttc
    16321 ggcatgggga acgctgccgg gttgttattc gccggaatta tgctgggctt tatgcgtgct
    16381 aaccacccga ccttcggtta cattccgcaa ggtgcattaa gcatggtgaa agagttcggc
    16441 ttgatggtgt ttatggcagg cgttggtctg agcgccggta gcggtattaa taacggcctg
    16501 ggcgcgattg gcggtcagat gttgattgcc ggattaattg tcagtcttgt gcccgtggtt
    16561 atctgtttct tgttcggtgc ttatgtattg cgaatgaacc gcgcactgtt gttcggcgca
    16621 atgatgggcg cacgcacctg cgcgccggca atggagatca tcagtgatac agctcgcagt
    16681 aacatccctg cgctgggcta tgcgggcacc tacgcaatcg ccaacgtcct gctgacgctg
    16741 gcagggacaa tcatcgtcat ggtatggcca ggattaggat aaaactgaag ttgccctgaa
    16801 aatgaaattt ttttgcacaa ccgcagaact tttccgcagg gcatcagtct taattagtgc
    16861 cactgctttt ctttgatgtc cccattttgt ggagcccatc aaccccgcca tttcggttca
    16921 aggttgatgg gttttttgtt atctaaaatt tatctactac cctgcaaccc tctcaaccat
    16981 cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg acaaaatgca gcgtcatccc
    17041 ctcaataaac gcatcaagcg cgcgggcggt tccgggttca aaccattgtt cgagcgtttg
    17101 ctgactgcgc tgcatccagt tttgcattac cgtttttaat agcggttttc ggctagccag
    17161 cgcgtagagc tggtacatca gctccatatt atccggcgtt gcaacctgtg agctgtagat
    17221 catatcggtg atagcctggc atgcgccttg agcatcacta acatcgctaa aaaatgcctg
    17281 atattgccgg gacataatct cagtaaaact gctgaacgcc tccagcaaca actcatcaat
    17341 tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg gcaagggtag caattttgcg
    17401 gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc agcgtggcct ggataatttt
    17461 ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt cctctgtctg caattgtgta
    17521 caaatgtaca caaccttgct aatgttgtct tccctcttct tattttgacg tgatctatga
    17581 ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg ggcgctgttt atgttcttct
    17641 ttttgccagg cctgttaatg gcgtcctggg caacccgtac gcctgctatc cgcgatattc
    17701 tctctgtctc gatcgctgaa atgggcggtg ttctctttgg tctgtcgatc ggttcgatga
    17761 gcggtattct ctgctcggcg tggttagtga aacgctttgg gacacgtaat gtcatcctgg
    17821 tcacgatgtc ctgcgcattg atcgggatga tgatattaag tctggcactc tggctgacat
    17881 cgcccctgct ctttgccgtt ggtctcggcg tctttggggc aagttttggt tctgcggaag
    17941 tggcgataaa cgttgaaggt gccgccgttg agcgagaaat gaataaaacg gttttgccga
    18001 tgatgcacgg tttttatagc ctaggcacgc tggcaggcgc tggtgtcggg atggcactga
    18061 cggcctttgg cgttccggca acggtgcaca ttttattggc ggcgctggta ggtatcgcgc
    18121 ctatttatat cgccattcag gcaatccctg acggtacggg caaaaatgct gccgatggca
    18181 cccagcatgg cgaaaaaggc attccttttt atcgcgatat ccagttgctg ttgattggtg
    18241 ttgtagtgct ggcgatggcc tttgccgaag gttctgccaa cgactggtta cccttattaa
    18301 tggttgacgg tcacggtttt agccctactt ccggctcgct gatttatgcc ggttttaccc
    18361 tagggatgac cgttggacgc ttcactggcg gttggttcat cgaccgttac agtcgcgttg
    18421 ccgtggttcg ggccagtgca ctaatggggg cgttgggtat tgggctgatt atttttgtcg
    18481 atagcgcctg ggtcgctggg gtgtctgttg tactctgggg actgggtgcc tcgctgggct
    18541 tcccgctgac catttctgcc gccagcgata ccggccccga tgcaccaacc cgcgtcagtg
    18601 tggtagcaac gaccggttat ctggctttcc tcgtcgggcc gccgctgctg ggctatctcg
    18661 gcgaacatta tggattacgt agtgcaatgc tggttgtact ggcgctggtt attctcgcgg
    18721 ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac gcggacggcg atggagaata
    18781 gttgatgagc attaaattaa ttgcggtaga catggatggt actttcttaa gcgatcaaaa
    18841 aacctataac cgtgagcggt ttatggctca gtatcagcaa atgaaagcac aaggcattcg
    18901 ctttgtggtc gccagcggga atcaatatta tcagttgctc tctttcttcc ctgaaattgc
    18961 taatgaaatt gcctttgtgg ctgaaaacgg cggctgggta gtgagtgaag gcaaagatgt
    19021 ttttaatggc gagctgtcga aggatgcctt tactactgtc gtggaacatt tgctgacgcg
    19081 cccggaagtg gaaattattg cctgcggaaa aaatagcgcc tatacgctca aaaaatatga
    19141 cgatgccatg aaaacggtgg cagaaatgta ttatcaccgt ctggaatacg tcgataactt
    19201 tgacaactta gaagatattt tctttaagtt tggcctgaat ctttccgatg aactgattcc
    19261 acaagtacaa aaagcattac atgaggccat cggcgatatt atggtgccag tccacaccgg
    19321 caacggcagc atcgatctga ttatccccgg cgtacataaa gccaatggcc ttcgccaact
    19381 gcagaaatta tggggaatag acgacagcga agtggtggtc tttggcgatg gcggtaacga
    19441 tattgagatg ctgcgtcagg caggctttag ttttgcaatg gaaaatgccg gcagcgcggt
    19501 cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt gaaggcgtac tggatgtgat
    19561 cgataaagtg cttaaacacg aagcgccatt tgaccaataa aaacggcagc acgcccggca
    19621 acacaatacc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc attatttcag
    19681 gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc tttactggtg
    19741 tggcgatggc gcaaagtgtc accggcgatg tgccgagtgg atacaaagtg gttgtagttc
    19801 ctgattcagt cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa accgtctatg
    19861 tggctcctgc gccagctccg gcttaccgcc cgcatcctta tgctcgccat cttgccagcg
    19921 taggtgaagg gatggttatc gaacaccaga ttgacgacca tcaccattaa ttcgagaatg
    19981 cctgatcgca caaagcagtc aggcattttt ttacccttcg tgagaatttc ccatctgttt
    20041 atcttttaaa aagataacca tcagcgacag ccacaaaatt ccgttgacaa ggttgaagag
    20101 attaaactgt ccgttgcccc cgttcagcca ggcatgtttg ctgatttcaa taccaacggt
    20161 aaagatcagc atttgcagca ttcccatcgc cgcagaaacc gtacctttac tcatatcgct
    20221 ggcaaacagg gttaatcgca ccagtcccgc attcgccaga ccaataccga aagcataaat
    20281 acttaacccg gcagtcatcc ataaatacgc gtgcgatgag ataaccgttg ccgcagcagc
    20341 gaccaacaga ccaatcataa tcggccagcc gcccataatt atcacgtacg gtgcggcgcg
    20401 aggtcagacg cgctaacagc aagttacccg caattaacgc cccgaaaata ggcacttgca
    20461 gcaagccata ttcatagctg ctcaactgct ccccggtaat gatgataatc ggcgactggg
    20521 cgatccacgc cagcaatggc aggctaacga atcccagcgc cagcgccccc gccacaaagc
    20581 ggccgttctt cagcaccagc ttatagtcac gaccgagttc tttcagtgac agtttctcgc
    20641 ctatacgcgt ggcggtttca ggcatagctc gttgcagacc gaaaaaggag atcgctgcca
    20701 atgcggcaaa caaaacaaac atcccctccc agggcagcac atggatccac gccgcgccca
    20761 ccagcggacc aagtagcgga gcaatcagcg ccacgttcgc catcagcgcg gtgatcttga
    20821 tacaaaccgc ctcttcgaag gattcctgaa ttgcggcgta tcccacagcg ccaatgaaac
    20881 agaggcttat gccctgcaag aagcgcaaca gggtgaattg ttcaatgttt tgcgccagta
    20941 atattgccag acaggtgacg ataaaccaca ccacacccgc cagcatcacc ggacggcgac
    21001 caatacgatc cgacagcggc cccaggagcc attgtaaaaa catcccgccc gccagatacg
    21061 cggtcatcga agtaggaacc caatcaatgc ccgcctgata ttgttccacc acggccaaca
    21121 taccgggttg gatcatatcg ttgccgatat aggttgaaaa ttcgtaaagc accagacaga
    21181 gagggaaaag taacgcctga cgtccaagcc tggcaccgaa agctaattta ttttgcatgc
    21241 aatttcttcg ccaataataa tcgcgcagag tttaataaaa gcgcagctaa cgagaaagcg
    21301 aattttgtag ctgaaaccac ggttaagcac attcttacat tattgcgagt atagctacgc
    21361 tttctttaag ttttatttaa cctatgcccg ttacaatcac ccaccgtaaa caggccgctt
    21421 gagggaaata agacgatgcc gctttaccca gtttaacctg cactttattc tcaacgactt
    21481 gcctgtattg gctccctttt aatcactttg cgtcgggaag ttatgctgga aaatttgaat
    21541 ctctctctat tctctcttat taacgcgacg ccagactcgg ctccgtggat gatctcgttg
    21601 gcgattttta ttgctaaaga tttgattacc gtggtgccgt tgctggccgc ggtactttgg
    21661 ttgtgggggc ttacagcaca acggcaactg gtgataaaaa tcgctatcgc gctggcggtc
    21721 agcctgtttg tgtcctggac gatgggacat ctttttccgc acgaccgacc ctttgtcgaa
    21781 aatatcggct ataacttcct gcatcatgcg gcggatgact cattcccgag cgatcacggt
    21841 acggtgattt tcacctttgc actggcattt ttatgctggc atcgcctgtg gtccggctca
    21901 cttttaatgg ttctggccgt cgtcattgcc tggtcgcgcg tttatcttgg cgtccactgg
    21961 ccgctggata tgctcggtgg attgctggca ggtatgattg gctgccttag tgcccagatt
    22021 atctggcaag cgatggggca taaactctat caacgtctgc aatcgtggta tcgcgtctgt
    22081 tttgcattac cgatccgcaa aggctgggtg cgtgactgat ctctgctaaa aagtgtagta
    22141 ttgagcggct cgcttcaata actattcaga gggattatgg aaacacgtcg cgaagagcgt
    22201 atcgggcagc tgctgcaaga attaaaacgc agcgataagt tacatcttaa agacgccgcc
    22261 gccctgcttg gggtttcgga gatgacgatt cgtcgcgatc tgaacaacca cagtgcgccc
    22321 gtcgttttgc tcggcggcta tattgttctg gaaccgcgca gtgccagcca ttacctgtta
    22381 agcgatcaaa aatcccgcct ggtggaagaa aaacgccggg cggcaaaact ggctgcgaca
    22441 ctggtagaac ccgatcagac cctctttttt gactgtggca ccaccacgcc gtggattatt
    22501 gaagcgattg ataatgaaat cccttttacc gccgtttgtt attcgctaaa tacctttctg
    22561 gcgctgaaag agaaacccca ttgccgcgca tttctgtgcg gtggtgaatt tcacgccagc
    22621 aacgccattt tcaaccccat cgattttcag caaacgttga ataatttttg cccggatatc
    22681 gctttttatt ccgcggcggg cgtgcatgtc agtaaaggcg ctacctgttt taatcttgaa
    22741 gagttgccgg taaaacactg ggccatgtcg atggcgcaaa agcatgtgct ggttgtcgac
    22801 cacagtaaat ttggcaaggt gcgtccggcg cgcatgggtg acctgaaacg ctttgatatt
    22861 gtggtgagtg attgttgccc ggaagatgag tatgtgaagt acgcgcagac gcaccgtatt
    22921 aagttgatgt attaatgacg tataaccgga tgacgtttcg cgccatccgg ttatcagaag
    22981 attaagagaa ccagctgccg aaccactgat ggaatttcat catcacgaaa tcccacatcc
    23041 gaccaaagaa tccgccctct tccacatttt ccatcacgat cagcggacgc tgctcaatgg
    23101 atttaccgtt aagctggaaa tcaatggtcc cgacaacctg accttttttc agcggtgcgg
    23161 taagctgcgg ttccgttaac gtgtagctcg ccttcaggtt tttcagctgc ccacgcggta
    23221 tggtcacgga gcccgcttcg cctgccccga gattcacttc gctcttatca ccaaaccaga
    23281 cgcgctgagt cacaaaggtg gcatcaggtt taattggcgt cacagtttca aagaagcgga
    23341 aaccccaggt caataatttc tcagactcat taaaacggat acggtcagtt ttcgccccca
    23401 gcactacgga gattaaacgc atatcaccct gggtagccga agcaaccaga ttatatcccg
    23461 cacctgccgt ggttcctgtc ttcatgccat caacattcag attgctgctc cacagcagac
    23521 ggttacggtt aggctgacga attttgttga aggtgaactc tttctcttta tgaatggcgt
    23581 actcttccgg cacatcgtgg atcaatgctt tacccagcaa tgccatatcg cgcgcggtac
    23641 tgaactgccc cggcgcatcc agaccgtgca ccgtctggaa ggtagtgttg gtcagaccca
    23701 gttttttggc ataaccattc atcaaaccaa taaatgactc ctggctcccg gcaacgtaat
    23761 cggccagcgc aatacaggcg tcattaccgg actggataat cacacctttg ttcaagtctg
    23821 ccaccgaaac ctgatcgccc ggtttgagga acattaccga tgaaccacgc agtgccggat
    23881 ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc ggtgagttta atcttatcgg
    23941 ccttaagcgc ctgcccaacc acatagctgg tcatgatttt agtcaggctc gcgggatcca
    24001 gtttctcatc cgcgttgcct tctgccagca ctttaccgct ggcgtaatcc attaaaatcc
    24061 atgcacgcgc atccacgctc ggcgcttcaa cggtttgttc cgccgcgaat gccgttgggg
    24121 caaaaaggaa taaaaatgca gaacccgctg caagaccacg aaggagagag gagtattgcg
    24181 tcataaatgc cacccgagta tccattcaaa aaaaatacgt tatatcgccg tatgataagt
    24241 tcggtgatta ataacgcaca cacaaactta aagaaaccgt aagttggtaa agtttttaaa
    24301 gtttatgcaa tatcctgccg cttcgctgct tctggtgtca tttttttgcc agctgttgct
    24361 taaaagttaa ctttacttca ccatcggagt cctgttcagc ctgtggctgc ctgtcggaaa
    24421 aggggtgagt atgattacgc tgtggggtcg gaataattca actaacgtaa aaaagtattg
    24481 ctgacgctcg aagaactgga actaccttat gagcaaattc tcgcgggccg tgagtttggg
    24541 ataaatcacg atgctgattt tctggcgatg aaccctaacg ggctggtgcc gttgttgcgt
    24601 gacgacgaaa gtgatctcat tctttgggaa tcaaacgcca ttgtccgcta tctggcggca
    24661 cagtacgggc aaaaacgcct gtggatcgac tcaccggcac gtcgtgcgga agcggaaaaa
    24721 tggatggact gggcaaacca gacgctcagt aatgctcatc gcgggatcct gatgggatta
    24781 gtcagaacac caccggaaga gcgcgatcag gccgccattg atgccagttg caaagagtgc
    24841 gacgccctgt ttgccctgct cgatgcggaa ctggcaaaag taaaatggtt ctctggcgac
    24901 gagtttggtg tgggcgatat cgctatcgca ccgttcatct acaatttgtt taacgttggc
    24961 ctgacctgga caccgcgtcc aaatctgcaa cgctggtatc agcaactcac tgaacgcccc
    25021 gcagtgcgca aagtggtgat gattcccgtt agctaattgc gtgggctaac tttaagtaat
    25081 tccccactgg actcgtcggt gagaacgtat aaataaccgt cgggtccagt gcgaacatca
    25141 cgaattcgct gccctctgtc cgttaaaata cggccatctt ctgtcacttt gtcgccgttg
    25201 acgctcatca caatgacatc tttatctttc agcgcgccaa taaataattt ttgctgccac
    25261 tgggggaatt tatcgctgtt atagaaggcc atgccgctca cagcgggcga atctttccag
    25321 taaaaaacag gttgctcggt cccggcgacg atctcccctt tcgcttccgg tatcttaaag
    25381 cctgaatagt tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg
    25441 atattaattt catcaccacc gcgcgggcca tgttcattca gccacagtgc attactccac
    25501 ggattcatcg ccattccttg cggattacga atgccataag accagatctc ggcgcgcgca
    25561 ccggattcct ttataaaagg attatcatcc gggatttcgc cctggtcggt cagacgcacc
    25621 agtttgccct gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga
    25681 gcaataaaaa gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta
    25741 gacagttttg gcatctggcg gaagacggtg cggaagtcgg tcacttttga gagatcatca
    25801 cttaagcggc cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa
    25861 cttaaccaga tgcggcgaga ctgagcaaaa tcaggcgcta aaaccacgtc cagcaggccg
    25921 ccctgcccgt gcgcccaaac gtccggaact ccggaaagcg gcgcagataa tccttttcct
    25981 gcttgccagt gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt
    26041 aaaaaggcca gtgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt
    26101 gcaggagccg cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt
    26161 cgatgcataa cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca
    26221 aattcatttt tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc
    26281 aggccttctc aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca
    26341 gacaattttc aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac
    26401 tcatcaaaca tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccact
    26461 cttcctgatc cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt
    26521 taaaaaagtt atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc
    26581 tcagttgcac gtatgcagcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga
    26641 ttcgctgtct gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcgattaaa
    26701 tagtatggct attttttgag caaaatgctg tccaggatca attcttcacg ccaaattaac
    26761 aaaatttaca tttccttcgc ctccgccagc gaatgagatt gcgatacaaa ttttatccag
    26821 aactctgtag aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc
    26881 aaagtaactc cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc
    26941 gattcagagc gtattctcac cgaactacgc actgaaggtt atgacgtggt accgagctat
    27001 gacgatgcgg acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa
    27061 tcactggaag ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt
    27121 ctgggggcaa aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg
    27181 cctcatagct atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac
    27241 aacccattcc tgagcctggt gccagaacaa ggtgtgaaac tgacaccgcg tcattatgcc
    27301 tatctgaaaa tttcggaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg
    27361 cgcggcgacc tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggta
    27421 gatgcgggcg ttaaagagat tctggtgatc tcgcaggata cttccgccta tggcgttgat
    27481 gttaaacatc gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg
    27541 tgcgaacagt tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg
    27601 catgtggacg acgtcatccc actgatggca gaaggcaaaa tcctgccgta tctggacatt
    27661 ccgttgcagc acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat
    27721 cgccaactgg cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca
    27781 acctttattg tcggcttccc tagcgagacg gaagaagatt tccagatgct actcgacttc
    27841 ctgaaagaag cgcgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca
    27901 gacgccaatg ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt
    27961 ttcatgcagc tgcagcagca gatttccgcc gagcgcctgc aagagaaagt gggccgtgaa
    28021 attctggtga ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat
    28081 gcaccggaaa tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat
    28141 atcctgcgtg tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa
    28201 ttaattcagg ccgggcaaaa tcatttgtcc gccctgcgaa taatgattac tgccagctaa
    28261 tctgagccct gccgtttttc ttactcttat acagccgctc atccgccagc attttcaagc
    28321 gttcgacggt ggtacattca tttgaatgcg ctacaccgat actggtactt aaattaatgc
    28381 ttcccacctg gaaatgaacg atttctttta gaatcgatag caactcttcc agcgttccac
    28441 ccgtataaat caccgcaaat tcgtcaccgc cgaagcggta gattttacct ttatctaccg
    28501 ggatctgtga tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac
    28561 cgaaggtgtc gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt
    28621 tttccggcag cgcttttaaa tcttcgtcaa aggattttct gcccattgct ttggtcaggt
    28681 cgtccaggaa cgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga
    28741 aaagcccgcc atccagtacg cgttcaaaat ggttattaat gatggcttct ttctccacaa
    28801 taccacgatt aatcactctg cgtaccagat agaggcaact ggtacccgat atcactgcca
    28861 ccaatgcggc aaagaaaata atcaatgtac agacccgcaa ataaacatct tttttatacg
    28921 caaatacgta caatccggtc ggcaaatagg cgaagtgctc catcgcttcc gaaacgatga
    28981 agatggcgtt tttagcatta tattcatcac cggtttaccg ttcttaaata gagagaacat
    29041 caggttctcg ctgtccatcg ccagcgattt tgacagtaaa gaaaaacgct ccagaccaag
    29101 caaaaatatc agactacgat tgtgagttac cggatagagg taatagacga aggttttacc
    29161 gtcaaaagag ataaaccctg tatttttgac atgtcgatac ttcacaacct gcataatatc
    29221 tgcacaataa tttttctcga actcctttac ctgaatacat tcgtcactgg aaatcagata
    29281 aggaccgaca tcgtacaaat aagaggggga aaccgagtcg gcgtaagctg tgcgcaaacc
    29341 aaccaacagc tgttcctccg tgggatttag cgtgttgata agatcaattg ccacgctacg
    29401 ccgatattca gcaatatttg ccaccgcaaa agcagtatca ataacggact gtttcaggct
    29461 atacacccgt tcctttacca tttgagtgta gatcccgcag gcaactgctg aaatcatgat
    29521 aaaaatcagc agactgactg taagtacgaa cttattgatt ctggacatac gtaaactact
    29581 cttttactaa ttttccactt ttatcccagg cggagaatgg catgggtttc tggtacagat
    29641 acccctgagc ctgaacacat cctaatttaa ttaattcgcg cgcaacgtct tcgctctcta
    29701 cgccttccgc aatcaccggg atccggttat agcgtgccag atcgaaaata atgcgcacaa
    29761 tttttcccgc agtaccgaaa atatcgccgg taaattgcga gtccaccttt atgctgtctg
    29821 gattaatttc ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca tcaatcgata
    29881 tccgtacccc ttgtttacgc agttgttcaa tattgagata aaaatgttca ttaattcgct
    29941 cgatcacggt ttcagtgatc tcaacacaga ggctgttcgc cttcaggcca ttttctttca
    30001 tgatggtggt aaaacgctca tgaaagtgcg gttcgtttaa ctgccagggc gaaacattaa
    30061 tatgcagtaa gaaatcatcc ttcagcgacg cacgctgact aaaatgtctg aattcaccca
    30121 ttgccgtttt aataatctga taacccagct cattgataaa cccgatatct tcagcaatgg
    30181 taatgaactt cagcggtgaa atgatccccc gctccgcaga aacccaacgg cacaacgcct
    30241 ccgcgcccag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg aggaatagtt
    30301 cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct tctatttcgt
    30361 tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg atactggaaa
    30421 tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa ggttcacaaa
    30481 tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa tcgtttacca
    30541 tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa tagagatcgg
    30601 cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc tgacacaatt
    30661 tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacggct tccagaccac
    30721 acagtttgat gatccccaca gccagatact gattattgtt ggactcaata atcgccatat
    30781 ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta tttgaccgtt
    30841 caatttcggc atctttcatt tccgcgatcg aa
//
LOCUS       DAZRVY010000038        30829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZRVY010000038 DAZRVY010000000
VERSION     DAZRVY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.038"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            34..372
                     /locus_tag="ACHXOX_001788"
     CDS             34..372
                     /locus_tag="ACHXOX_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR7005271.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQADL"
     gene            383..670
                     /locus_tag="ACHXOX_001789"
     CDS             383..670
                     /locus_tag="ACHXOX_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005272.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            682..924
                     /locus_tag="ACHXOX_001790"
     CDS             682..924
                     /locus_tag="ACHXOX_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR7005273.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            921..1034
                     /locus_tag="ACHXOX_001791"
     CDS             921..1034
                     /locus_tag="ACHXOX_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005274.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            1121..1324
                     /locus_tag="ACHXOX_001792"
     CDS             1121..1324
                     /locus_tag="ACHXOX_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005275.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            1321..1566
                     /locus_tag="ACHXOX_001793"
     CDS             1321..1566
                     /locus_tag="ACHXOX_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR7005276.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            1592..1711
                     /locus_tag="ACHXOX_001794"
                     /pseudo
     CDS             1592..1711
                     /locus_tag="ACHXOX_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1708..2073
                     /locus_tag="ACHXOX_001795"
     CDS             1708..2073
                     /locus_tag="ACHXOX_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005277.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            2080..4902
                     /locus_tag="ACHXOX_001796"
     CDS             2080..4902
                     /locus_tag="ACHXOX_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR7005278.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            4979..5918
                     /locus_tag="ACHXOX_001797"
                     /pseudo
     CDS             4979..5918
                     /locus_tag="ACHXOX_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            5923..6234
                     /locus_tag="ACHXOX_001798"
     CDS             5923..6234
                     /locus_tag="ACHXOX_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR7005279.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            6295..6819
                     /locus_tag="ACHXOX_001799"
     CDS             6295..6819
                     /locus_tag="ACHXOX_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005280.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            6816..7055
                     /locus_tag="ACHXOX_001800"
     CDS             6816..7055
                     /locus_tag="ACHXOX_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005281.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(7747..8793)
                     /locus_tag="ACHXOX_001801"
     CDS             complement(7747..8793)
                     /locus_tag="ACHXOX_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7005282.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(8793..10544)
                     /locus_tag="ACHXOX_001802"
     CDS             complement(8793..10544)
                     /locus_tag="ACHXOX_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR7005283.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10699..11535
                     /locus_tag="ACHXOX_001803"
     CDS             10699..11535
                     /locus_tag="ACHXOX_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR7005284.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11559..12611
                     /locus_tag="ACHXOX_001804"
     CDS             11559..12611
                     /locus_tag="ACHXOX_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFR7005285.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            12657..13457
                     /gene="gpM"
                     /locus_tag="ACHXOX_001805"
     CDS             12657..13457
                     /gene="gpM"
                     /locus_tag="ACHXOX_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR7005286.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            13559..14053
                     /locus_tag="ACHXOX_001806"
     CDS             13559..14053
                     /locus_tag="ACHXOX_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR7005287.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            14053..14250
                     /locus_tag="ACHXOX_001807"
     CDS             14053..14250
                     /locus_tag="ACHXOX_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR7005288.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            14244..14546
                     /locus_tag="ACHXOX_001808"
     CDS             14244..14546
                     /locus_tag="ACHXOX_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005289.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            14527..15075
                     /locus_tag="ACHXOX_001809"
     CDS             14527..15075
                     /locus_tag="ACHXOX_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR7005290.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            15072..15545
                     /locus_tag="ACHXOX_001810"
     CDS             15072..15545
                     /locus_tag="ACHXOX_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005291.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            15617..16084
                     /locus_tag="ACHXOX_001811"
     CDS             15617..16084
                     /locus_tag="ACHXOX_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005292.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            16077..16712
                     /locus_tag="ACHXOX_001812"
     CDS             16077..16712
                     /locus_tag="ACHXOX_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR7005293.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            16709..17290
                     /locus_tag="ACHXOX_001813"
     CDS             16709..17290
                     /locus_tag="ACHXOX_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR7005294.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            17287..17637
                     /locus_tag="ACHXOX_001814"
     CDS             17287..17637
                     /locus_tag="ACHXOX_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR7005295.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            17641..18537
                     /locus_tag="ACHXOX_001815"
     CDS             17641..18537
                     /locus_tag="ACHXOX_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR7005296.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            18530..19060
                     /locus_tag="ACHXOX_001816"
     CDS             18530..19060
                     /locus_tag="ACHXOX_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR7005297.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            19063..21099
                     /locus_tag="ACHXOX_001817"
     CDS             19063..21099
                     /locus_tag="ACHXOX_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005298.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNATNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNKAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTD
                     SYMWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALR
                     IFNSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGD
                     IFANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQF
                     GIYMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGV
                     NNWQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            21101..21628
                     /locus_tag="ACHXOX_001818"
     CDS             21101..21628
                     /locus_tag="ACHXOX_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7005299.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(21657..22190)
                     /locus_tag="ACHXOX_001819"
     CDS             complement(21657..22190)
                     /locus_tag="ACHXOX_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7005300.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(22193..22936)
                     /locus_tag="ACHXOX_001820"
     CDS             complement(22193..22936)
                     /locus_tag="ACHXOX_001820"
                     /inference="COORDINATES: protein motif:HMM:NF024018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR7005301.1"
                     /translation="MCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIRVTT
                     PSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAVGWN
                     WNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEADWSE
                     FYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTGSVN
                     SNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            23218..23805
                     /locus_tag="ACHXOX_001821"
     CDS             23218..23805
                     /locus_tag="ACHXOX_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7005302.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            complement(23841..24329)
                     /locus_tag="ACHXOX_001822"
     CDS             complement(23841..24329)
                     /locus_tag="ACHXOX_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005303.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(24342..27149)
                     /locus_tag="ACHXOX_001823"
     CDS             complement(24342..27149)
                     /locus_tag="ACHXOX_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7005304.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(27136..27291)
                     /locus_tag="ACHXOX_001824"
     CDS             complement(27136..27291)
                     /locus_tag="ACHXOX_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR7005305.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(27300..27665)
                     /locus_tag="ACHXOX_001825"
     CDS             complement(27300..27665)
                     /locus_tag="ACHXOX_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005306.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            complement(27720..28232)
                     /locus_tag="ACHXOX_001826"
     CDS             complement(27720..28232)
                     /locus_tag="ACHXOX_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR7005307.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            complement(28232..29416)
                     /locus_tag="ACHXOX_001827"
     CDS             complement(28232..29416)
                     /locus_tag="ACHXOX_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR7005308.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            29574..30683
                     /locus_tag="ACHXOX_001828"
     CDS             29574..30683
                     /locus_tag="ACHXOX_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR7005309.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
BASE COUNT         7696 a         8425 c         8086 g         6622 t
ORIGIN      
        1 cgtgcagatg acaaaataac gaggtgtcat tttatgctga agcaacgccg taatttttgt
       61 accggaacag aacgccacgc taaccgtttc actaccagtg catcacgcag caacatccgc
      121 tacagcctga gtgatacaca cgcaacgccg gatggctacc cagtaaaaca aatcggcgag
      181 cacgcctggc tgattgagaa agctggaatc gtgatccaca aatgcccacg caatccgttt
      241 accggaaacc gcatttttgc actgagctgt ggcgacaatc agttcgggca ggatttcaca
      301 ttatacgaag cacttcgcac ggttgatcgt ctgcttcgcg ggcaaagttt tattaaacag
      361 gctgatttat aacaggtgct ttatgaccaa agacaatgca caaggtgtat ttatccgttt
      421 tattgatttt cgcggtgaac tgttattacg tgcatccgct attgacggag tgactccggc
      481 gggtaaaaac ggagccgacg aagccactta cgtttatctg aacggcacgc gactgcttgt
      541 ggaacttccg taccagaccg tacgagaaat catcagcgaa gatgaaaagg cacgccaggt
      601 taatggcgat gaaccctata tcgaaattat ttgtatggat tcagaagctg aaatacagaa
      661 agcagattaa agggcgttgt gatgggcaaa gaatataaaa ctctcattaa caaagcactt
      721 gagcgttttt attttcgctt aagtgcatca ggcgctcatg ctgaacgtgc ggcccgtgac
      781 tcattgacca gagcaatccg aagtctgtat gacgtggctt tttacgctga tgatctggat
      841 gcacttaacg aactttccga gctgatctgt gccgcagaat gcggggaaca tattgaaccg
      901 tataagctgg gaaatatcgc atgagtatat ttatttcatg gcttgttctg attatttcgg
      961 tggtctgcgc cattgggatt atgcgaatta ttaattcagt aaaaaagatt gaacgctttt
     1021 tcactgaaga ataaccgcgc aaataagacc ccaggttaaa taagaaaatg tgaaaacaat
     1081 ccgcattcgc ggaggtattc gcacacgcca aggaggcgta atggcaatta agcattttcc
     1141 tgtcgttcgt ttcacctcca gaggacgtga atacgaagtt gacgaacgcc tgattaccac
     1201 aatcgacaaa caccgttcag aaaaggatgc acatcatatc tatctcactg acggcactta
     1261 cttctgcgcc accaacgtgg tgcaggtgaa tcttatcaga caggtacagg agtcacgcag
     1321 atgaccattc tggactacat cgctgccaat ccgggttgta gcagtggaga aatcgccgca
     1381 gcactgaata caccaaccac aaccattaat gcggagctac gccgtctctg gcgcagcggt
     1441 ttagtcacaa gaaaagagcg caaaacaggc ggtcgctttt cttatcaggt aaacctgatg
     1501 ccgtttgggt gtagcaaccc actaacccag atgttcaacc agctactgag ggaaatcaga
     1561 gcatgagcac ctccaactgc cggaaaccac gtcgggcttc agcagctcat ccggcagcaa
     1621 aacaaactcc attaattcct gttccgggcc tttcctgcac cttgcggcgg gaggccttcg
     1681 cacatctgta acaagaggat tgccgcaatg attctcgcca acgactttct tgaatacctg
     1741 ctcaacacag agcgtgatct tgccgctcgc gtgcgtgatc gttatgacat gtacctgaaa
     1801 tccctgcctg taccgcagct cgctgacgga aagattgtta ttgatggtcg ctacatgatt
     1861 gacagccacg agggaaatta caggctttac cgcattgaag gtggcacccc gtccgttatt
     1921 ggcatttacc agcgcccatc ctctgcaatc gtcgatgtga ttgccgacag catccgcatc
     1981 acacatcgcc atgccgacac agaagacacc gtgctggaaa ttcagcggct ggctacagtc
     2041 tgccgcgaca ccctgaatgg catgacgaag taaatcacta tgacggcaga gtacatcagg
     2101 gactggcaac aaccgcgcca cgcagtgggg cgtgaaggaa cggggatccc cgctcctgaa
     2161 tccgcgcttt cctcctggct ggatgcccac cgggcagaga acgagcgccg ccaggaaatg
     2221 gctgatgcgg cgttctccgc cacgccactg ggcaacctga ttaataaaag cctggacgca
     2281 caggaaaaac aggacaaaac catcacactg gcaggagacg ccagaaaaca ggcacgcggc
     2341 gcggtggatg aagccatggc ctcgctgcgc ctgctgccgt cctatctgcg cgatccgctt
     2401 attcgccacc tctccttcct gcgcaaaaaa caggaagccg atcgccggaa aggcaaaaag
     2461 agctggcagg cggaacgcta tgcacgcgga accctgcgca aaatattcga acgtctggat
     2521 cgcactgacg gacactggct gacaccgggt tatcgctccc ttgccggacg tgaacgcctg
     2581 gacgatttgc tttacctgcc gcaactcaac aaacaccaga tacagacgct ggccaccatg
     2641 acggcggcga tgttcagcag caccttcgaa aaactctgcg atggttttgg cgcgactgat
     2701 ggcgagctga ccatggatgt aacgctgaag gcgtatcaga tgctggcccg catggcgtta
     2761 cacttacacg ccatgcctcc gcattatgac gcactgacaa cagacaaaga ccggaggcac
     2821 gaaccggaca cagaactgct gccgggcgca atccttcgcc tgacctgtgc ggaatggtgg
     2881 aaacgcaaac tgtggctgtt acgttgcgag tggagagaag aacaacttcg cgccgcctgt
     2941 ctggtttcca gaaaaacatc gccctatctg agccaggacg cgttaagcga gtttcgcgca
     3001 cagcgcgaga aaacacgcga tttcctgaaa agtttcatgc tggaaaatga agacgggttc
     3061 acgattgatc tcgagacggt gtattacgcg ggagtaagta acccggttca ccgtaaggca
     3121 gaaatgatgg ccaccatgaa ggggctggaa cttctggccg aagcccgtgg cgacaaagcg
     3181 gtgtttctga ctgtcacctg cccgtcaaaa taccacgcca caacggagaa cggtcatccg
     3241 aatcccaaat ggaacggggc cacaatgcgc gactccagcg attacctggt taacacgttt
     3301 tttgcgacgg tccgcaaaaa actgaaccgc gacggcctgc gctggtatgg catccgcacg
     3361 gcggagcctc accatgacgg caccgtgcac tggcatatga tggtctttgc tcatccggaa
     3421 gaaatcgaca ccattgtgtc ccacacccgc gatattgcca ttcaggaaga tcgtcacgag
     3481 ctgggcgatg acataactcc gcgctttaag gcggagtatg tagacggctc aaaaggcacg
     3541 ccaaccagct acatcgccac ctacatcggg aaaaacctgg acagccgcgc cgtggatggc
     3601 atcgacccga aaacgggcaa gccacgcgtt gaccacgaaa ccggaaaatc aatggccgag
     3661 agcgtggaac gcgccatcgg ctgggcgcgc cttcaccggg tccgccagtt ccagttcttt
     3721 ggcatcccct cccgccaggt atggcgtgaa ctgcgccgcc ttgccagcca gatggcacgc
     3781 aacccggaag gcccgcaacg gctgaaggat gacgcaatgg atgcggttct tgccgccgct
     3841 gatgccggat gttttgccac ctacatagag aaacagggcg gcgtacttgt tccacgcaaa
     3901 gactacctga ttcgcaccgc ctacgacctc gccgatgagc tgaacgatta cggcgaacag
     3961 agcgtacaga tttacgggat ctggtcacca ctcatcgggg aatcctcccg tgtgtgcacg
     4021 catccggata actggaagct ggtaagacgc aaaccggaag cggaagacag cgcccgcgaa
     4081 aatggttttg accttcaggg cggccctgcc gccccttgga ctcgtggcaa taactgtccc
     4141 cgtgtacagg aaacggacaa caacgggaca gaacagccgg aagaacggcc agcaccgtgg
     4201 ccgcagctcc ctgacggcgt tgatgtggat gaatggatgc gctcactgaa acggcacgaa
     4261 cgccgggcgc tgatgcgttc gcttcgtgac aaacaggcaa aaaacagcag tgatgaaatg
     4321 cagagctgga cacagagccg caaacagcag cggcctttgc ctgataacca cgaattactc
     4381 gctaaagaat ggcgggagtc tgctgaatct ctcggcctgc atatcggtga acaacagatg
     4441 cagcacctgt tacggggcgg cagtctgtac gttgacggca gcatcattgc accgcaggga
     4501 tttgaaattg tacgcaaacc ggatacccgc ccggacagcc gaatcacgca gctctggcag
     4561 cgcctgagcc gtaatcatgg cgtaagcagc acggagatcc gccataaccc ggtcgccagc
     4621 tatctgacac agctgggggc atcagaccct gaagccgccg cacgcctggc atccacactt
     4681 cagcaggacc agaacaccat gaaaacaccc gttaccgtgc tttctgacat gctgcgcgcc
     4741 atccgcgacg cagagcacgc acagagaatc agtgaaacca ctgaacgcgc ccgccgcaaa
     4801 gcagacctgc tgcggggtgg cctgaccagt ggaaacaaaa aacagacaga aacgggactc
     4861 acgaatcccg taaatgagca aaaaacgcgc agcgatatat gaagcgcgca caaaacaggc
     4921 aaaaacggga tttcagaatc ccgtaaacga ttaattaatc aacataagga aaagcgacat
     4981 gaaaatttgt atcgacgacg gctccaccaa catcaagctg gcatggactg agaacggcga
     5041 acgccgcaac gccatcagcc cgaacagctt caagtcggaa tggtctgcgc cgttcggtgg
     5101 catgcagccc gcgaactaca tgcttgatgg cgtgcgctat ggttttgatc cggtcagcga
     5161 tcgctttgtc cagacaaccg acacgcaata ccagtacagc gatgtaaatg tcattgccat
     5221 tcatcacgcg ctggtcaaat caggcatcac gccacaggag gtggatgtgg ttgtcaccct
     5281 gccactgagc gagtatttcg acacaaacgc acagccggac atggccaaca tcaaccgcaa
     5341 aaaagcgaac gttatgcgcc cggtggagta ccagaacggc gaagcattca ctatccgtaa
     5401 cgtgcgggtt atgcctgaat ccattccggc tggctttaag gcactggctg acatgagtcc
     5461 gtttgaatcc ctgctgattg tggatttggg cggaaccacg ctggatgtgg caaaggttca
     5521 ggggcaactg gcaggtatca gccaggtgtt ttgcgatcca cacgtaggcg tttctctgat
     5581 ggccgatgcc gtactgtcgg tgatggccac taacggtatg cgcaccagtc accacatcgc
     5641 caataccatt atcgaacatc gccatgatga agcctggctg cgccagcaca tccacaatga
     5701 cgcgcattac gccagcctga tggcggttat tcgtgaaaag gaagaaacac tgaaacaacg
     5761 cgtgatccgc gcgctggcgg gtgatggttg tcggtggagg ggcggagatt gtggcacccg
     5821 ctatccgcga agcctgcgga gttaatgcga ctttcatcgc ggacggggtg ccacagtttg
     5881 ctctggttaa tgggctgtac gcaatggaca aggagtaaac caatgacgac accaaccaga
     5941 cggataagtt tctatctgaa gcctgcggcc gtcaagaacg aaagcgaagc atgcgcctgg
     6001 ctgggcagcc ttacaccaga agcccgcaaa agcggccaac gcgtggcttt tctggccggg
     6061 ctggcacttc tgaaaatgaa tccagcagag gcttaccgac tggctgcatg ggctgacgat
     6121 gaggcgttat cggtgacaca aaccaggaca gaacgccccg tgccacagcc agtaccaacc
     6181 gcacagataa ccagtcagat agcagggaat atccgggcat tatttcctga atgacgaatt
     6241 tgacggcttt taattttatc ggggtgcact gtcgccccgc acgaggaaaa cacgatgaaa
     6301 aaatacgaat acatgctaat gaaagaagca ctgcgggctg gtctggctcc ctgtcctgta
     6361 tccgtagaag ctgttgttgg gaacacgatt ttccgaacgc tgcgaaatcg cgtatccgtg
     6421 aagatggttc accaaccacc caaatctacc agcatgatct atctgccgct gcccgaggct
     6481 atcaaactta tgcgttcgct gaaatttgcg atttggatgc agacacaaat caacaaagcg
     6541 gtgcaaaaca ccttcgttaa gagactaata ccggagcttg ctgccagatg tgcaaaatgt
     6601 accagacccg cacttcaatt tgatctgtca ttgattcaac aagctacgga ggcagaactg
     6661 taccgcctgt cacctatccg tgatgctcac acggaagcat cagaaatgtt ccggctcatg
     6721 tttcaattta attttgccgt tcgacgcaca cgcgttcagg tcaatcgcat gatgcaggag
     6781 caggaagagt taacacagta caaagggggc agccagtgag taattcagca cgactacagc
     6841 ttggtttttc accgctatca aaaaccatcg tattggcaaa aatgcgtgac ttaggagacg
     6901 gaacaaaacg ccgcgtagga aatgatcgcg gtcgcgatgt aaccaacgag gccgctcaac
     6961 tcgtttggca tctagtcatg gcagaaggcg gtgaaattaa ttgggagctg gatgatggtt
     7021 ctcgtatgat tttgagggcc gagaagagta aatagctcaa gggggattaa tgctaaatga
     7081 tgatgaagaa gagctgctta tgcaggaatg gtcgctcggt gattatgata atggtgaaaa
     7141 tgggtgcccg cattgtggcc gttatcgttt atgtatctgc cagaatggaa aacacagatg
     7201 tgaaaagtgc aattggtctc cggaacttaa tgactacgtg ccgatcgagt ggtagaggta
     7261 agtcacacta ggtgtttttt agttgatttt ataaatgcac aatagtgcac aaatttgcac
     7321 aatttttttg aacgactttt tgcccttccg gcccgcgtgg cggctggatc cgtcaaggat
     7381 ccgtgcatgc acaaaaaaac gcgttttttc tgcgcgcagg tgacggggga acagcccgcg
     7441 tttcaggggg taaatagcat tccctgaacg atgtcgcaga gatacaacag aatggctgta
     7501 tttctcacgc tgagcgtgaa aaagacgtga gggcttttga tttgatgggg tgaaaggtaa
     7561 ggccgtcaaa atcgcactga gacggcgaga atatgcagtc aacgaggtgg gattgcgtaa
     7621 gagtctgact gtcgatgatg gcaatcagca ggaaagcgtc gtgaaattat ctgactgata
     7681 caggagctgg agagtcgggg cataaatttt ttatgccccg gcgaagcagc agacaagcga
     7741 agcgcgtcag gatgtgggct gggtgtctaa cagtgcgtaa gggttaaagc ggatcacctc
     7801 ttcgccaagc cagtcattga tgtgcttcat ggcctccatg acgggcatca gctcgttaat
     7861 tgcgtaaacc cgcgcggcct tctccacatc accaaacgca cttttttcgc ccggcatcgc
     7921 ccccatcagt tgcggcggaa cgcggtgcgc agccagcaca tcatcacggg atgccgcctt
     7981 aacattcatg aactcatcct ttgcggtgat ctgctggaac ggcaaaattt gcaccccctc
     8041 tttgcccccg ttgggcgcat gaatgagcac gtttttaaac gcaccaccac cacgtgcccc
     8101 ctgtagcgtt tctttcaggg agtccatgct ttcgcggttt acctgcgctg caccgatgta
     8161 gatgatgcac ccggcgtggg atccgttgtc gtagtacagt tttctgaaca tgtccgccga
     8221 atgagaaagg ctggccgaga gtaatgcgcc aagatattcc ggcatgccgt agatttcctg
     8281 gttaatatcc ggattcatca ggtggcacac tttgccagga cgaaactgaa acgcatcctt
     8341 gccatcctgc acataccacc atgattcaag atcgcttccg cgtcgcatgt atttcgccag
     8401 ggcgtgccgt aatttaagcg gttcgccgag catattgctc cgaagctcaa ggaatgcgtt
     8461 accgaacaca aaccagtcca gcgccagcgc cgagaaatcc tgccgggaaa gcagcgggtg
     8521 cggaatatag cagccgagca atacattgcg cttaaagtaa agcgcagact gatgccagga
     8581 cgtttgccgg gcagctcttg ccagaccgta ccagtccacc ggggtttcat accaccgccc
     8641 gttatcagca cagtacatat tgtccagcag gtcatgcccg gtcaggcggt aaggaccatc
     8701 aaatgtgaat gcactgagcg acgattcttt cctgagcgca tcagcgagat caatgcgtga
     8761 actcatgcgc acttttttat tttttctgct catcagaact ccataaccgt gaaacgctcg
     8821 ttttctcctt cgccgccaat cggttcgtta atgacagcaa gcatggttgc ccacgcaagg
     8881 tcgccgtggc tgatccccct cgcgcggtcc gtttcgtaag tgataaaacc gcccggtgtt
     8941 ttcaccttac gcacggcgtt aaaggccgca accagctcgc gttcggcgcg atcgtattcc
     9001 caccgcccgg cacgcattat ttgcagcatt ttcagtacca gcgaccgttt tgatgacagc
     9061 gtgaaggtgt aagggatggc ggcgggaaaa aaccgtttca ctatctgata gacggcatcc
     9121 ccgtgcccgc ccgtcgcatc aatgccgatg tgttccacgt tgtagcgata cgtgaactct
     9181 tcaataactc tggcctgctc ttcaaattcc agcccctgaa cgcgtcgcgt ctccaccgtt
     9241 ctgaaacgac caccaggaac agccggagga accaccacgg acacagcgcc gctgtcgccg
     9301 ttaccactgc tgccgtttgc gtcataccca atccataccg gacgattccc catcgggcgg
     9361 ggggcaaagg gtttccagtc tttccagtcg tcgtatccgt caacaccgca gccaatcagg
     9421 atattcaggt taaatgccga ttccccttcg cggacaaact cacacatata gagattgcgg
     9481 aactcgtctt cggtgttttc atcacgaatt tcatcaatat cggtgtgttt ccagccgtga
     9541 ttaaccacat cttccagcgt gacaatttgc cgccacgtcc ggtcagggca gataagcccg
     9601 ttatgcagcg ttttccagtc cacagaaaaa cgctggcgtt tatgcgcggc ctttttctcg
     9661 ttccagcggt cgccgttcca gtaggcgtat gcctcgtgcg tttcggtgga tggcgtggag
     9721 aagcaggtgc gccgcagtcc gctgagggtt gccatagcgc cagccacctt gcgcagttca
     9781 gcaaagcgac tgacccagaa aaattcatca aaataaaaat tgcccgtgta agattgcgcc
     9841 gtcgcagcag aagtgccgag aaaatgcatc tctgcgccgt tggagaggat gattttatcg
     9901 ccccctttca gctccacatc gacttcagcc gcggccttct gaataatgct tttaaactgg
     9961 aacgcctgac gacgcgacgc agacaaaaaa atctggttac gctggtaagg ttgtgccaca
    10021 tcgtcacgca gcgccatcag cagtgcttcc tgtgcaaaat accaggtcgc cccaatctgt
    10081 cgggatttca ggatcatcct gttacgtatc ccggcttccc tgcaaagcgt cagggagtca
    10141 aaccagcccc gctgatgcca ctccagcctg ctgatgattt tttcccgcag tgcggcaatc
    10201 tgttccggcg tgaaatgatt tttgagtttt ttcgcccggc ctttctttcc tgtggccgtc
    10261 gcatccggct ggccatcatg cagtttttta agctgccggg tcagcaggtc tatttcctta
    10321 aagtcaccac ctgttttatt ctgtttttca gtaagctgga tgaggcgcgc atcgatggac
    10381 tgcgtgacac gctgcacggg tggcgtttca tcccactggt cgcgtttttt ccacgcataa
    10441 atcgtgttcg ggtttattcc catcagacgt gatatttctg cgggcggata accctgccag
    10501 taaagttgcc gcgcacgctg gcgcacaaaa gcgtcctgaa tcattgctcc ccctgagtaa
    10561 ttacaggaag attacccgcg cgcgaaaccg ttctccttaa ccccctgttc tggccgtttt
    10621 cttacaacaa aagccctttg tatcagcctg ttacgctttg ccatcatgac tgaagaacca
    10681 gtcagagggg caaaaactat ggctaatgaa aaaaagacat cccgcaaaaa gtttcgcgtg
    10741 gctgtctccg gatcaactgt tgatggccgt gaaatcagtc cggtgcatct gcgtgaagcc
    10801 gccgagaact tcaacccgga tgtttacgct gcccgcgtga acgttgagca ctatctctcg
    10861 ccatgcccgt caagcgaatt ttccgcaatg ggcgatgtca ccgcactgag tacggaagac
    10921 attacggaag gtccgctggc cggacgtact gcgctgtatg cagaaatcga accgaccgag
    10981 cgcatgaagc agcttgtcgc ggacggcaag aaaatctatt ccagtatcga actgcacccg
    11041 cagttctccg ttaacgggcg cgcctatctg gtcgggctgg cgatgaccga caccccggca
    11101 agcctgggca ctgagcgcct gaaattcacg gcacagcaac gtcaggcggt gatgacgttc
    11161 aacagtatcc agggtgaagc accgcttatc tccgaagcca tcgagtctga aatcatcgaa
    11221 atggcagaac aacgccagga agaaggcacc cagtggttta accgcgtaat ggggattatt
    11281 ggtcgtggcc gcaaagcgga tgacgccagt ttctcccgta ttcaggaagc ggtggaaagc
    11341 gtcgcaacgt cacaggccga cattatcgac cgttttaatg cgctggaaac acgccatcag
    11401 caggacagcc agaaaatcac tttactgacc acggagctga cagcactgaa ggaaaaactg
    11461 cgcacgcagg acggcgatcc gcagaaccgg ttcaccgcaa cgggcgcagc ctccgaccag
    11521 ctggctgact tctgataaga caaaggagca aattttttat gaatctggtg atgtcagata
    11581 ttacccgcaa caagctgggt tgctatatgg cgcagcaggc gtcgcttaac aatatcccgg
    11641 tttctgcact ggtatcgcga tttaccgtgg aacctgcggt gcagcaacgt tttgaaaacg
    11701 cctcaaagga tagcaccgaa cttacaaaaa aaattaacgt gatcggcgtg accgaccaga
    11761 aaggcgaaaa aatcctcctg gacaccacag gaccgattgc acgcacaaat accagttatg
    11821 acggaacaaa acgccgtaac ccgaataacg tggttgatct gaaaaaccgc aaataccagt
    11881 gcgaacaggt gaactacgac acgtttattt cgtatccgca gcttgatgcc tggtcggcac
    11941 acagcgattt tcagtcccgc atcagcgcac agattgcccg acaggtggca cttgaccgca
    12001 tcatgatcgg tttcaacggc acatctcacg cggatgagtc caacttcagc accaacaagc
    12061 tgcttcagga cgttaacgtg gggtggctgg agcacatcag aaccaatgcc agcgagcgcg
    12121 taatgaatga cgtgacgctg acttcccgca acatggacaa caccgtggcg cacgcgggta
    12181 aatacgcgaa ccctgatgca ctggtacagg acgcgcgctc atctctgctg gatgaatggc
    12241 acaaggaagc tgacgacctc gtggtgatta tggggcgcaa cctgtttaac tcgctgcgtc
    12301 tgccagtgct gaacagcatc agcggccaga atcccaatgc ggaattactt gccgggcagc
    12361 tcatcctgtc atcgcgcacc attggcgggc tgggtgtgtt ccttgcaccg ttcttcccgg
    12421 atgcaacgat gctgatcacc tcattcaaca acctgtcgat ttactggcag aaaggttcaa
    12481 tgcgtcgtct gatgaaagac gaaccggaat acaaccgcat cgccacctac cagtccatca
    12541 atgacgctta tgtcgttgaa gactatggca agtgcgcgat ggtcactggc ctgaagttcg
    12601 ccgacagcta atcaactcac ggcgggcatc atgcccgcct gtaacggaga gaaaaaatga
    12661 ttactcctgc acagcaacac tggcagaacg tgatggcaca gcgcgcaggc cgggcgaatg
    12721 aaggtgtgga ccacgccgcg cgtaccgcgc atgaagaggt gctgtatcgt ctgcgtctgg
    12781 cacaggcccg gcttaagggc gtacaggcca gaagcgcgaa agccgacatc aaaaaagcgt
    12841 tgttgccgga cttttccggc tggattgagg gaacgctgga ggctgacggc gggcaacagg
    12901 acgaagtgat tgccacgctg atggtgtggg cgattgactg cggcgatctt ccgcttgcgc
    12961 tgcgtattgg tgcgtatgcg gtccgtcaca acctcatcat gccggataac tttggccgta
    13021 ctgctgccac agtgctgacc gaagaaatct gcaatccggt actgacgcag gccgggacgg
    13081 atgccgacgc ggatttgtcc gtctttatcg aaccactgga cacactttgg gaaattgtcg
    13141 ccaaccagga tatgccggac gaagtgcgcg ccaaattatg caaggcgtgc gcctttgccc
    13201 gccgtggcct gagcgatgcg gacagcatgg cctcatcact gaagctgctg cgcgaagcga
    13261 tgcatctgaa ccagaacgca ggtgtgaaac gcgagattgc aaccctttcc cgcgccctga
    13321 aaaaagccga ttccgcagcc gcaccagaag acgccagcgc acagcaggcg caggacgaaa
    13381 gcagcgaaag taaaaagaca acgcggaagc ctgcaacacg aaaaaccacc gcgacgcaga
    13441 aggcgaagcg cggttaacga ctgaccccgt cagcgggcgg cgtgcgcggt gttccggttt
    13501 ggctccgtga ccgtttacac cgcgcaccca ccgcccgatt ttttcaggag tgaaccccat
    13561 gagtatggtt gccagaactg aacccggacc cgcagaggac gacatcaccg acaccgaaga
    13621 tggcgatacc cgtatttcag cgggtgcatt ctggccggat attgtgctgc gcgagctgcg
    13681 tctggcggta cgactgccgg gccgcgtgac cacctcccgc ctgctgcata ccgccaccgg
    13741 ggccgtggca cacgttaccc gcgagctgga agcatggcag caggaacagc aggcggctgg
    13801 ccatcagacg ctggccgatg ttccggcacc tgtaattaac ggagaaagcg tcaatctctg
    13861 gcactggcgc aatgctgttt ataccgccac acgcgccctg attctggagc gttaccgcga
    13921 tgcggacaca acggacaagg gcgaccgccg ggcggacgca ctggatatac agacatcgga
    13981 tttgtggcgc gatgtgagct gggccatctc tgacattctg tgccgcccgc gaatctttgc
    14041 ggagttgtgc tgatgaaagt gaagacactg gaaggcgaca ccgtggattc gctctgtttc
    14101 cggtactacg gcacgacgca gggcgtcacc gaaaaggtgc tggatgccaa ccccggactc
    14161 tgccgacagg tatttctgga agccggacag gaggtggaaa tgccggagcc ggagaagaaa
    14221 aaacaacgag agatgataca gctatggtaa caggtttttt tcagcgggtg agtgactgga
    14281 tcaccttctg ggcatcagtc acagccacca caataggcgt gatgaccatc agtgaaaaaa
    14341 tcgcccttgc cgggttgtta ctcggttctc tgtctgcatt acatgcctgg cgacatcgcc
    14401 gcctgatgga aaccgccatg cagcgtcgca atgaactcat cgccagtatc ctggcgcagt
    14461 cagacgcacg gccacttcac gacagtgagc gtcaggctct gaacattctg cgggataacg
    14521 atacacatga taaagctgcc cgttaaacgt tacgccgtag ccgccattgt ggcgctgggg
    14581 ttctccattg cccctcacaa actccggacc agcccggaag cgcaaatccg cattgccaca
    14641 tgggaggact gtcgcgcaac gccttaccgg gatattgccg gggtaatgac cgtggggtgc
    14701 ggctcgaccg ggcatgtgga aaatcgtctg tacaccgaaa cggaagtcgc cgggcgctgg
    14761 gttaatgata tgcaacatgc agaaaactgc ataaatcaga acttcagcgg caacgccatg
    14821 ccacagtccg cgtttgaggc aatgacagat gccgccttta atcttggatg cagaaatctt
    14881 atgtggttca gggataagaa ccgtaactac caccgcacca ctatctggaa acatgcacag
    14941 gcgcacaact ggcctgacat gtgcaacaga ctgactgact tcgtgaacag cggcggcgaa
    15001 cgctcacagg gactggttaa ccgtcgtact gattttcgtg catggtgctt acgtgacacc
    15061 ggaggccagt catgaaagtg cgttacatcc tgatgatggc actgaccgtc ctcacgctgc
    15121 tgaccataac actctggcag acattcagac tggggcagga agacggacac aaccgttata
    15181 cacaggcgca actggacgat tacagccgga ccctgaaaga ggccggcacc atcatcagca
    15241 ccgcatccgg tgtactgcgt gacgtcatga cagcgcgcca gaaccgacaa caggaagggg
    15301 aaatccgacg tgaacagcta cgcaacgata tcacgacaga tgcatgtgcc cgcgctgttc
    15361 ctgacgctcg ctttactgag cggttgcacc agcacgcaga acgcgtccgc gccgccgccg
    15421 tcgttccgtt tcatcccgga agggctgacc gcccccgtac ttcccccgcc gtttccggtg
    15481 acgccatcat ggggaaacat cgggatatgg agcgacagcc tcatggacgc actggaaacc
    15541 tgtaacgccg ataagcgggc cattgaatta ctggaacagc gcaggctgca acgactgaac
    15601 aacgaggaca acaaccatgc tgaaaactga ttccctgcgt gaagccatga cccgttcatg
    15661 ccggtggtgt catgccaacc cggaaaaatt caccattttc gtggagagcg gcaacattga
    15721 aacgaccgga gaaaccccat cgtttgttta ccgctatcag atagtgatgt ttgtcatgga
    15781 ttacgccgga gagctggacg acctcacgct gccgctgctg gcgtggttat ccgaaaatca
    15841 gccacagttg ttgctcaatc cggagcgtaa tcaggacatc aaattttccg ccgttatcaa
    15901 tgacgatgac agcgccgatc tcctgtttac gctccccctg cgggaacgcg ttcgcatcac
    15961 gcgcagcagt cagggcacac cgcaggcaga acacctgccg gagccaaaac cccgcctgcc
    16021 ctcttccgaa ggcgactggt cgcatgtatt ccaggatgtg acgtgggggg aaagcgatgg
    16081 ataaggcatt cacccgcgtg gatgaaacct ttgaggctat ccgcgacagc ctgaatcagc
    16141 aggccattaa taacatcgcc agaaagctgg cacaggattt acgccgcgcc cagcaggcgc
    16201 gtatccggtc acagaaagcg ccggacggga ccgcatggac accacgcaga cgccgcgtaa
    16261 cccggataca ggagcgcatt cgctttatct ggaataacga agcacgcacg ctgaaaaact
    16321 ggcatcacga cacggggaaa tacgggcgaa ccatcaccgg gtgggatgag gataaaaaca
    16381 atatccgcac gttttaccgg gatgacatcg accgttttct ggaaatacgc acccggcgca
    16441 tcaaccagga cagcaccagg cgcgtctcca tgttcgtaaa actgcgcacc gcccgctacc
    16501 tgaaagcccg tgcagatgct tccggtgtga cggtgggtta cagcggcgtg gccgcacgta
    16561 ttgcacgcgt tcatcagttc ggtgagcgcg atcaggttgc gccgggcatt ttcaccgatt
    16621 acccggtacg tgagctgctg ggtatcagcc aggcagatga gcgcctgatt tataacacgg
    16681 tgctgggccg gattgcggag gctgtacgat gagcgcagaa ctcatgcgac tgctgagcaa
    16741 tatcatccgc accgggatca tctctgaagt tgatgaggaa tcctggtgcg tgcgcgttcg
    16801 cagcggcgaa ctggaaacag gctggctgcg ctggaacacc acgcgcgcgg gagacttcaa
    16861 cgtgtggttt ccgccatcac caggcgaaca ggtggcaatt gcctgcatcg gcggcaaccc
    16921 ggaaaccgcc atgataatcg gcagcctgtg gagtgatgcc attccggcac ccggcaaaag
    16981 cctgaaagaa atcgtggtca gcgcgccgga cggcgcggtg ttccgctacg acgcggacgc
    17041 aggcgcactg agcgcaagcg gcatgaaaac ggccactttg caggcatcca tcagcgtgac
    17101 actgaacacg cctgtcgtgg aatgcacaga ccttctgaga acagcgacgc ttgacgtcac
    17161 aaaaggggga acgatgagcg gcaatatcac gcacagcggc ggcgatttca cctcaaacgg
    17221 catcacagtg catacgcata agcacggtgg cgttaaaggt ggcagcgatt cgacaggagg
    17281 cccgcagtga caacccgcta cacaggaatg aacccggacg ggacgggaaa cctgaacgat
    17341 atggcgcacc tgaaacagtc agtcagggac atcctgacca ccccgctggc aagccgggtt
    17401 atgcgacggg aatatggcag ccttgtgcct gatttgattg acgaacccat gaataacacc
    17461 acgcgtctgc aatgcatgag tgctaccgtg attgcactga cacgatggga accacgcatt
    17521 gccctgaacg ccatcgacgt tgtctggaag gcaggaggcc acgccggggt gacgctgtcg
    17581 ggcactgtca tgcagaccat gcagaatgtt gaattaacca tcacgctgag ggagtaaatc
    17641 atgcctgccg ttgacctttc ccagttaccg gaacccgcca tcatcgcgga gcctgacttt
    17701 gaggcaattc tggctgacac aaaggccatg atgattgcag cttatcccgc cgaacagcgt
    17761 gaagtcgtct ccgccgcact ggaactggaa tcggaacccc tgaacgttat cgctcaaacc
    17821 atgtcgtttc gtgaaatgct gttacgccag cgggtcaatg aggccgcacg cgcctgcatg
    17881 ttgagccaca gcgccggaac agacctggac aacctcgcgg gcaatatgaa cacaaagcgc
    17941 ctggttatca ctccggcaac ggataccacc gacgtggtga tggagaacga cacctcgctg
    18001 agactgcggg cgcagcgggc gtatgacggc ctgagtgttg caggcccgtc aggcgcatac
    18061 gagtattttg cccgcagcgc cagcggtctg gtgcgcgatg cgcgggccat cagtccgtct
    18121 ccggcaaatg tgacggtttc catcctgtcc actgagggcg acggcacagc aacggaggcg
    18181 ttgcttaata ccgttcgcgc cgttctgaat gcagaggata cccgcccggt ggccgaccgc
    18241 ctgaccgtac agagtgccag aatcgtgaca tggcggctga atgcaaaact gtacttttac
    18301 cccggtccgg aatccgaacc tattctggct gcggctgaat cgtcgttcag gaagtggctg
    18361 gctgagcagg ggcttatcgg tcaggacgtg gcgttgtccg ccattgctgc cgcactgcat
    18421 gtgcacggtg tgcaacgcgt ggagataatc gaacccacac agaatatggc catcagcgac
    18481 atacaggcgg cgcgctgtga gtcattcacc atcagcgaag gtgggcgtaa tgagtaattc
    18541 actgttacca ccatcagcca gcaatttcat gcgttgtgcc gaagccgtcg gaacacgcat
    18601 tacagacatt ccggcagacc tcaacacgct gtggtcgccg gacacctgcc cggtgcatct
    18661 gctgccttat ctcgcctggg cgttttccgt tgaccgctgg gatcgcaact ggccggaaga
    18721 gacaaagcga caggtgattc gtgatgcatg gctgatacac cgacacaaag gaaccatcag
    18781 cgccctgcga cgggccattg agccgctggg atacctcatt cgcgtgtctg agtggtggga
    18841 gttcaacggc gaacccggaa cattcaagat tgatgttggc acgctggaca gtggcgtgac
    18901 ggaggaaatg tatctggaaa tggagcggtt gattgctgat gcccgcccgg tcagccgcca
    18961 catgacaggg ctgaatatca ttcaggagat tcagggagat attttcgcgg cagcagcaac
    19021 ttacgacggt gaagtcatta ccatttatcc ggacgattaa gcatgagtac cacaacacga
    19081 aaatttaaaa ccattatcac cgatacgggt gcaaaaaaat tagcgcaggc aaccgcgcca
    19141 gatgggaaac ctgtccgcct gacacatatg gccgtgggtg acggcggcgg tactttaccc
    19201 acgccagaca gtaagcagac ccgtcttgtg catgaggtgt ggcgacatac cgttaatcgc
    19261 gtcaccctgg acgcaacaca tcagaaccac attattgcgg agctggttat tcctcctgaa
    19321 acgggcggat tctggatccg ggaaattggt gtgtttgatg agcacggcga tttgatcgcg
    19381 gtaggcaata ctgccgaaag ttacaagcca accgttgccg aaggatccgg acgtgcacaa
    19441 acatttcgca ccattctgac cgtatccagc actgccactg tggcgcttac cgtggataac
    19501 accatggtga tggccacagt gaattacgtg gatgacaaac tgaaagagca tgaacagtca
    19561 cgacgtcacc cggacgcctc gctgaccgca aaaggccttg tccagctcag caacgccacc
    19621 aacagcacgt ctgaaacgct ggcagcgaca ccaaaagccg ttaaggcagt aatggatgaa
    19681 acgaacaaga aagccccatt aaacagcccg gcactgaccg gaacgccaac aacaccaact
    19741 gcgcgacagg gaacgaataa tacccaaatc gcaagcacgg ctttcgttat ggctgcgatt
    19801 gccgcacttg tagactcttc gcctgatgca ctgaacacac tgaacgagct ggcggcggcg
    19861 ctgggcaacg acccgaattt tgcgaccacc atgactaacg cgcttgcggg taagcaaccg
    19921 aaagatgcca ctctgacggc gctggcgggg cttgctactg cggcagacag gtttccgtat
    19981 tttacgggga atgatgtcgc cagcctggca accctgacaa aagtcgggcg ggatattctt
    20041 gcgaaatcga ccgttgccgc cgttatcgaa tacctcggtt tacaggaaac ggtaaacaag
    20101 gctggtaacg ccgtgcaaaa aaatggcgat acgttgtccg gtggacttac ttttgaaaac
    20161 gactcaatcc ttgcctggat tcgaaatact gactgggcaa agattgggtt taaaaataat
    20221 tcggatgcag acaccgattc ctacatgtgg tttgaaacag gcaacaacgg caatgaatat
    20281 ttcaaatgga gacaccgcat cattggcacc cggcctaaag acctgatgaa tcttaaatgg
    20341 aatgctttgt ctgttcttgt tgaagccctt ttcagcagtg aagtgaaaat atcgacagtc
    20401 aatgcactga ggatatttaa ttcatctttt ggtgccattt ttcgtcgttc tgaagaatgc
    20461 ctgcatatca tccctacacg agagaatgaa ggggaaaatg gtaatatagg gccactacgc
    20521 ccctttacgc ttaatctcag aactggccgg ataagcatgg ggcatggtct tgatgttaca
    20581 ggggatatat ttgcaaaccg ttttgcaatt aacagtagta ccggcatgtg gattcatatg
    20641 cgtgaccaga atgttatttt gggacgtaat gcggtatcca ctgatggtgc tcaggctttg
    20701 ctccgtcagg accatgccga ccgcaaattt atgattggcg gtctgggaaa taagcaattt
    20761 ggcatctaca tgattaataa ctcaaggaca gccaatggca ccgatggtca ggcgtacatg
    20821 gataataacg ggaactggct ttgcggctcg caagttattc ccggcaacta tggcaatttt
    20881 gattccagat atgtgaaaga tgttcgactt ggttcacagc aatattatgg agtgaacaac
    20941 tggcaaacat ggaattttca gtgcccgtca ggctatgtac tgacggggat taacgtacag
    21001 gataccggaa aaaactctgc cgataatatt gcaggcgttc attacaggcc agtgcaaaag
    21061 tatataaatg gcacttggta taatgtggcg agtatttaat atgatgcact taaagaacat
    21121 aaaagcaggt aatgctaaaa cagtggagca gtatgagtta acaaagaagc acggagtcat
    21181 ctggctttac tctgaggacg gaaaaaactg gtatgaggaa gtgaagaact ttcagccaga
    21241 cacaataaag attgtttacg atgcaaataa tattattgtc gccatcacca aagatgcctc
    21301 cacgcttaac cctgaaggtt atagcgtcgt tgaggttcct gatattacag ctaatcgtcg
    21361 tgctgatgat tccggtaagt ggatgtttaa ggacggagct gtggttaaac ggatttatac
    21421 ggctgacgaa cagcaacaac aggccgaatc acaaaaggcc gcattgcttt ccgaagctga
    21481 atcagtcatc cagccgctgg aacgcgctgt caggctgaat atggcgacgg acgaggaacg
    21541 cacacgactg gaagcatggg aacgctacag tgttctggtc agccgtgtgg atacggcaaa
    21601 gccagaatgg ccacaaaagc ctgagtaaaa attaaggccc gatatcgggc cttgtttcat
    21661 tctggttgtt cgggaaacgt tactggcagg ctggaggtgt ctgtggattc gaccttctgc
    21721 gcatagagca tccactcggt taatttttgt ttattctcgt cggaaataat gcccagccgt
    21781 agctgtgagt cccatagctg ggttttatcc ctgacgagct gtagcaggct ttgcttttca
    21841 ttttccgctt gttgcctctg ctcttccccg gtataagttc gctttatcac tacgccatct
    21901 ttgaacatcc atttacctga aatatcagcc cggcgatttg ctgtaatatc aggaacctca
    21961 acgacgcttg cgccttccgg attaattgct gaaacatcct tttcaataca aataataacg
    22021 ccgttgtggt catagaccat tttcaacgta tcaggctgaa agttcttttg ttcctcatac
    22081 cagtttttcc catcctctgt ataaagccat ttgatgttaa attgtttcgt tagctggtat
    22141 tgctcttttg tttcaggatt accagcagaa atgtttttta agtgcatcat cgttaaatac
    22201 tccccgcgtt ataccacgtc ccattaatgc aatactgaat tggccttgcc tgagttgtat
    22261 caattagttc atctctgttt gagttaacgg aaccggtaac aacataacct gacctgtcag
    22321 accatccggg accattccat gtctgaacag ataacagacc accaaggcga atacctgtaa
    22381 taaaccttga gttacattct gcctgcgtat atgcaccaac atctcccgca gagggtttgc
    22441 gggttgtggt gtaaaactct gaccagtcag cttcaaagcc ataaccatca cgcgctgaac
    22501 gataaaaaat accgccgttc ctgtaattca cgcggaactg tacagcaggg cagctccccg
    22561 tattcatatt gaagtggagg attaatgtcg atgcgccacc aatgtttgcg ttatagaccc
    22621 cgctattcca gttccagcca acagctttat catttccgac agtgcttcct gtttgcccta
    22681 aagcaaatgc aggttgttga tttttcgtgt tgtagtctcg tcgccagcca ggagcgtaag
    22741 catcaccatg attaatataa gtgaattgag cgttagtaat tccgccaccg ctggacgtgc
    22801 tcggcgtggt aacgcgtatg gtcattgcgc cgcgagtgcc aataacttcc accacagcac
    22861 ctgcaagaca aatatttccg caacctgtat ctgtaatgac cttattattt gcataagccc
    22921 atgagccttt gcacatccag taaggatggt taaatgctcc ctgactctcc agccacgaaa
    22981 taaattgcgc ggttgtccag acctgactat cgccaccaat attcagccat gagctatatg
    23041 cgcggcaggc accaatattt ttggtgaagg tatctttccc cggaatatct gcgccgttct
    23101 ggtttttctg taatgcgcca gaagcctgat ttaccgtttc ctgtaaaccg aggttttaga
    23161 taatggccgt ttccggcctg catggcatga tttgcgcttt tggacgggag attcagcgtg
    23221 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
    23281 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcgggac aaagacagac
    23341 cgaccgggat taaaacgcgc tttaaagcgc cttcaaaaag gtgacacgct ggttgtctgg
    23401 aaactggatc gcctcgggcg aagcatgaaa catctgattt ctctcgtcgg ggaactacgg
    23461 gagcgaggaa ttaattttcg cagtctgacc gacagcatag atacatcttc tccaatgggg
    23521 cgttttttct tccacgtgat gggtgccctg gctgaaatgg aacgtgaatt aattgttgaa
    23581 cgtacactgg ccggactggc ggcagcacgc gcacgggggc gcacaggcgg acgtcgaccg
    23641 aagctgacaa aagaacagca tgagcaaata gcaaggctga tcaaaaacgg gcacgacaga
    23701 aaacaactgg caataattta cggcattggt atatcgacga tttatcgtta ccaccccgca
    23761 ggagaatcaa tcggaacaat agagaagagt caggaaacaa aataaccgct aatctgacca
    23821 ttagcggttt ttgtgttaaa tcagaacaac cctttaactg aactggccgc gctgttaagg
    23881 gatgatgtta ccttatcttt gaagccggac agcatatcgc tgaacgatga ggattgcagg
    23941 cgctcccgca aatcctcatc acagcgttca agagtcagtg aaaattctat ctttttcgcc
    24001 ttaccgtagc gatcaaactc ggagcgggtc gtattcgttt cggtcaggac atacatgccg
    24061 taaatctgcc cgacgccatc aatcagaggc cagggtcgtc ctgtatacgc ctgcgtggtc
    24121 agcagcgaca gcgacacttc gccacctgta atttcaggat aaagcacacc agaaagaacg
    24181 atgcgctcat cacctgcacc gatatactgc cagcttgccg aacggttaac gcgttcattt
    24241 ttcacatgcc gccagctttt gttttgctgt aactgctgat gcggcaatgt acgcagctca
    24301 aaaacaaaca tgccgtagat catcatcatg gccatgactc ctcaatcttt atcgtaaaaa
    24361 ctgccacgcc cggcacgggc gcgccgttcc atttctgccc tgaccatttc accgaccagt
    24421 ttcgccagtt cgcggggatt ctgcgtaaca acgttatgca gatgaacatg aatttcacca
    24481 ccaaatccgg aggcaacagg ctcccggtta cgggaagtta caggaactga tgccactgga
    24541 gagcgtatgg cctccgccac cgggcgggag ctggccgcaa caacagggac cagcgccgga
    24601 ggcagcggag ccgggaccac gggtgtgata ttaattgcgg gggcaggctt actgacctgc
    24661 gcaatcttcc gctcctgcca ctccccacga acagcaagtg cgcggggcag gttcttaaag
    24721 acaatatcgc cggggccaat gcgttttttc gtctcatcaa ccagcttacc tgtgttatca
    24781 gcaattttgc tgagtctgcg tagcgtcccg gtattgctgt ctgtgagcgg tttgttgtct
    24841 ttgggtttat cacctccggt gccattgcca ttttccacag gcttcggcgg attgattttc
    24901 gccaggtccc cctgaagcaa ggcaaccttg tcctgaagaa tggccgcacg ctgtgcgtct
    24961 tcgattttct tgcgcgccct ttccgcttca tccggaagga cgccaagttt ttcaagtatc
    25021 cacgccagcg tatccagtag catttttgca ggtgtcagaa caagttgtaa cgcaccgcca
    25081 agaacgttac cgaatatctc gccagcactg gcacatttat ccagcgtttc cttgctggac
    25141 tccatcggtg acagcagcga tttaaaccag ttaaagacct gaataatccc atcgcgcacg
    25201 acatcaaaaa cagggccaaa ccgttcaaag gtttcgcgca acggggtcag cctttccata
    25261 atcccgctga acaccccggc aaaaaatgcc ctgatgggat cccagtattt ccagataaga
    25321 acggcagctc cggcaagcgc agccacgata agactaaccg gactgaacag cgccccgata
    25381 gcgccaccaa gtaaagaaac ggaacccgtc accattcccc ataatgcagg cagaaccctg
    25441 acaacattca ttgattcggt caggagagaa aaaccgagac gcaacgtagc cagcttcccg
    25501 tgaagcaccc caataaccag agacaacgaa ccaaccgttg ctgttgtcgc cagcagtgcg
    25561 cctcccgcta tcagtaactg gcgcgtcagt gccgggtggg cctgcgccag cgccgtcacc
    25621 tttgagacaa cccgcgtgag ccactgcgtg acagaacgca gcggaccgtc aaccagatca
    25681 ctgatgcgaa tacgcagacc ttcccatgca ctgccgagtg atttcaggtt gccatcaagg
    25741 ttattggcca taacctttgc cgtgcgttct gcctcaccgc gcgcgccttc aagttctttt
    25801 ctcagtttgg ctaagaagcc gtcacctgcc gcatcaacga ggcccataaa tgatgtgaaa
    25861 gcctcttctc cggcaatgtc cttaaagaac gatacccggt caacctcccc gtatttgcgg
    25921 gtggctttat agagatcggc cagcacattc tccatcgggc gcattttgcc tttggcgtca
    25981 gcaactgaca cccccagctc tttcagtgct tctgccgctg cctttggcgg tgatgacaga
    26041 ttggccaggc ttttacgcaa cgctgtacct gcatcactcc ccctgatacc catcttcgcc
    26101 agcacgcccg ccatcgctgc ggcctgctcc agcgatattc ccagcttacc cgccaccgga
    26161 cctgcatatt tcatggtttc gcccagtgcg cgaaggtcag tgttggtacg ggtaaacgct
    26221 gcggtaagtg tgtcaccgac ccggtccatc tggtcagcag agaggccgaa ctgcgtcagg
    26281 atattggaac caatatccgc cgtctcgccg aggtccatac cgccagccgt tgccatgctc
    26341 agcacgccgg gaagcgcagc ctgaatggcc tgcggtgtga aaccagccat tgcaagaaat
    26401 gcctgtccac tggcggcatc gcctgcggtg aactgcgttt cagagccaag ttttaacgcc
    26461 tgctcacgca gcgccttaaa ctgcgggctg tttttatcga ttcgcgtcag tgcctgaaca
    26521 cgggacatct ctttcccgaa cccgatagcg ggctgcaaaa aacgcccggc agcatagccg
    26581 cccgctgccg ccgcacctgt tgccagcgca ccacctgttt tcagttttcc cgctgtttcc
    26641 tgcgcgcgtg aataccgctc acgcgcctgc gttacacgcg caagcgcccg ccgttcgcgt
    26701 tcaagctggt tgttgtattg tccggtgcgt ctgatggcct gctggatggt gttatcgctg
    26761 cctgtcaggg aaatgccgtg gcgtttcagc tctccgccaa gttcccgcat tttctgaatt
    26821 tcccgtgtgc gcgattcatt caggcgttca agccgggtgc ttaactgctg catcagcttt
    26881 tgttgttttt cgctgagcac tgtacccgtg cgttgtaact gattaagggc gttaagctgg
    26941 cgtcgtgctt tcacgatgcc cgcatccgct ttactgacag cgtcacgggc gcgctcaaat
    27001 gaacgcgcct gacgctcgag atttttgatc gccccctgcg ttcgctggat ggagtcacca
    27061 aactgcccca tcaggcggcg ggcgttttcg gcaggccggg tcagcctgtc aacggcgctg
    27121 aaagcgaccc ggatatcaag agtcttcatt gtctgcattc ccgctgcgaa gtgccgcccg
    27181 ctcacgccag ctaaccactt cgccgggcgt catcatgaag atttcggcgg gcgaccagtt
    27241 aaaaatggcg gcaatatccg ccacaaagtc ttctatgtgc tcaaagcaca caaccgtgat
    27301 caggcttccg tcgcctgttc gttcttctcg ccagagtccg caccgctcaa aaaatttacg
    27361 gcaaccacac ataactgaat aaagtcacgg gatgccattt ttttgatcgt cacttcatcc
    27421 agtcgcggtg atgtcacgcg tgacagcagc gtaaacatgg attccgcttt caaattcagc
    27481 acatcagaca gcgacaaatc tcgcagagat ccagcctgct caatagcccc ggtgatctcc
    27541 acatacgtga ttttttcgcc gcctcgctca attggttggg taagttttac gccacgctca
    27601 ctggtttctt tcacagtgtc agcaacgacc gtgttttcgg tatcgatgtt tttcgtctct
    27661 ttcatcagga aactcctttc agtcagaggc gacgcactgc gccgcctgca tattacttat
    27721 cagccaagcc cgagcgcgga acggatgcga tcgggcacaa tgtccctgcc gtccttccgg
    27781 taaatgaagt tcagcaggtc aatctcccac aacgggcgat cgttaacact cagcttgtag
    27841 taggtgtttt taatggcgta agtgtgtgat gtggcttcgc cctgtttggc ttcccccata
    27901 tcaatttccg tcacacgtcc gcgcatttcg acttcataca ggtcgctttc agcgtcggtg
    27961 tagtattcac ccgcaaaacg cagcagcgta ccgtcaatcg tgccgccata cttaaggaac
    28021 agctcacgaa ctgcgccccc catgacaaag ctcgcatcaa gcgcggagtc atccaggccg
    28081 agatcaatac ttaccgctcc catcatgcca ccaccccggt agctgtcggt tttgcgcgtc
    28141 agcttaggca gagtgacgga cgtcacctta cccacttcgt tttcaccatc cacaaacagc
    28201 gtaaaaaagc gaagatgttt tggtacagcc atcaggcacc tcccagcacc gcaaatgcgg
    28261 gaccaaagaa ttcatcagta aacgactggt aaagctccat gtcttccagc gggggaacag
    28321 gcgtatattt gtagcgaata cgcacgcgcc cctgacgtaa attcgtggtg ccgttatcca
    28381 ccacgtcata ccagcacgac gccccaatca gtttcccggc agtaaccagt gaatccagtt
    28441 ttgccctgat ggcaccgata acatccttca cgttcgcagg cgtcagtgga ctgtcgatgg
    28501 tttcaaactg cgcttccgca attgaatcag ccagcacctg tgcggttcgg gtatacacct
    28561 caaagatgta ggcgttcgtt tccggtgtgc ggttgcccca gaagcggaac ccgttgcgac
    28621 gaataatggt cgtgatttct ttgttgttga ggctgttggc atcactgtct tcggcctgca
    28681 acgaccagaa cacatgcctg gacatcccca gcacattttt aaccggaacg ttggacagcg
    28741 atttgtgcca gccctgctca tggtcaatgt acgcacgaag gccgcacgca taggcaggcg
    28801 cggggaacgt ttcgtttttg ccacttttcg ggttgtaggc gatgaagtcc ggccataaga
    28861 gcatcacctc acgttcgttg aatttctggc ggtaggtaat tgcctcagcc atcgtgttac
    28921 agtcatgaca tgaggcatac acaaacgcgc gcagtttacc cgcaatcacg cacagggatt
    28981 ttgtcaccgc ctccgtgtcc agctccggcg cggccagaat acgcggacgg tatccgatgc
    29041 tttcatcctg ctctgcaaca agcagcgcat acatccccgt atagctgccg tcagattcag
    29101 aaccaccgat aaccagttga tcctgcgttt ttccgtcttc ttctttgtgt tcagccacgc
    29161 gaacgacgat cacctttgtg ctcacctggt ctgcgatggc cttaagcgca cgataaagcg
    29221 tccccgttgt tccgcatttt cccagcacgt cattgacgcg gggcagcagt gtgggcttgt
    29281 tcagcgggaa cagctccgcg tccgcatcat ccgccgttgc cacgataccg ataacgctgg
    29341 aatcaacatc gttaatcgct gttaccaggt cggtattttc cgtaacacgg gcaccatgaa
    29401 aacgagtttc actcatagct tcaacccctt gtatccgtta aatgattcgg caacaatcat
    29461 cacccaccac gcgcgtaatc tcacccctgc gccattctcc cgccacggcg acaacaaaaa
    29521 gcagtaaccc cctccgcacg cacatgcgac catgccacac agggagggag cagatgaccg
    29581 acaccaccat gcaattgctc agtcagggca cagaccccgt gaaaatgccg gattttgata
    29641 ttctcgcgga gggtaaaacg ctgtcaggcg tggcagagcg cctgatgagc ctgtcactga
    29701 ccgacaaccg gggatttgag gcggaccagc tcaccatcac gctggatgat gcggatggtc
    29761 agttgcagct accgccacgg ggcgcgcgcc tgacggttct cattggctgg aagggagaac
    29821 cgctgacaga aaaaggcact tacattgttg atgaaatcgc tcacgaagga ccgccggaca
    29881 ggctgactgt ttcagccaga agcgcagatt ttcgggctga atttaacgtt aaacgtgagg
    29941 tgtcctggca tgatgtgacc gttgagcgtg tggtatccgc catcgctcat cggtatggtc
    30001 tgaaaccgca aatcagcgaa atgctgatgg atatcgaaat cgaccacgcc gaccagaccg
    30061 aagaaagcga catgtccttc cttacgcgca tggcggaaat gctgggcgca atcaccacgg
    30121 taaaaagcgg taatctgtta ttcatcatgc ccggtggtgg cgtgaacgca cagggccagc
    30181 cgttgccctc gttcgccatc acacgcagca gcggcgatcg ccatcagttc cgcattgctg
    30241 accgcgaagc gtatacgggg gtacgcgctt actggcttga tcttaattac gggaaaaaga
    30301 aaaaagtcag cgtgaaacgc cgcaaaccgt caaaaccaaa aaaggagaaa agcagcagcc
    30361 gggaaggcga ctatatggaa ggtgcggaag gcaacgtgtt tgtgttacgc aaaacttatc
    30421 agaacgagca ggcagcaaga cgtgcagcgg cggcaaagtg gcagcagcta caacgcggag
    30481 ccgcatcatt ctccatcaca ctggcgcatg gacgtgcaga actctacccc gaaatgcatg
    30541 gcacggtaac aggatttaaa agcgagattg ataatcagga ctggattatt gcaaaagccg
    30601 agcacaccat tgataacagc ggctttacca cgcagcttga gcttgaggca aaaatcccgg
    30661 aatggatagc agaaacagag tgagcaactt aaatgtatta actcagaacg gatcaacaca
    30721 ctcacaacat agagtcaata tagctgctcg ctttgttcca gcaaggatgc ctatttctaa
    30781 ggaaaaacag agcagaaaca tatggtgttt tcctacctga catcagggg
//
LOCUS       DAZRVY010000039        30824 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZRVY010000039 DAZRVY010000000
VERSION     DAZRVY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.039"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(131..496)
                     /locus_tag="ACHXOX_001829"
     CDS             complement(131..496)
                     /locus_tag="ACHXOX_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7005310.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(789..1774)
                     /gene="yqjG"
                     /locus_tag="ACHXOX_001830"
                     /pseudo
     CDS             complement(789..1774)
                     /gene="yqjG"
                     /locus_tag="ACHXOX_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            complement(1844..2236)
                     /locus_tag="ACHXOX_001831"
     CDS             complement(1844..2236)
                     /locus_tag="ACHXOX_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR7005311.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2422..2721)
                     /locus_tag="ACHXOX_001832"
     CDS             complement(2422..2721)
                     /locus_tag="ACHXOX_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR7005312.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2711..3115)
                     /locus_tag="ACHXOX_001833"
     CDS             complement(2711..3115)
                     /locus_tag="ACHXOX_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR7005313.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3118..3423)
                     /locus_tag="ACHXOX_001834"
     CDS             complement(3118..3423)
                     /locus_tag="ACHXOX_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR7005314.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3461..3829)
                     /gene="yqjC"
                     /locus_tag="ACHXOX_001835"
     CDS             complement(3461..3829)
                     /gene="yqjC"
                     /locus_tag="ACHXOX_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR7005315.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3976..4359)
                     /gene="mzrA"
                     /locus_tag="ACHXOX_001836"
     CDS             complement(3976..4359)
                     /gene="mzrA"
                     /locus_tag="ACHXOX_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR7005316.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4363..5025)
                     /gene="yqjA"
                     /locus_tag="ACHXOX_001837"
     CDS             complement(4363..5025)
                     /gene="yqjA"
                     /locus_tag="ACHXOX_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR7005317.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5047..5130)
                     /gene="yqiM"
                     /locus_tag="ACHXOX_001838"
     CDS             complement(5047..5130)
                     /gene="yqiM"
                     /locus_tag="ACHXOX_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR7005318.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5370..6146)
                     /gene="exuR"
                     /locus_tag="ACHXOX_001839"
     CDS             complement(5370..6146)
                     /gene="exuR"
                     /locus_tag="ACHXOX_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR7005319.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            6442..7158
                     /locus_tag="ACHXOX_001840"
     CDS             6442..7158
                     /locus_tag="ACHXOX_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR7005320.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            7188..7688
                     /locus_tag="ACHXOX_001841"
     CDS             7188..7688
                     /locus_tag="ACHXOX_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR7005321.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            7780..10464
                     /locus_tag="ACHXOX_001842"
     CDS             7780..10464
                     /locus_tag="ACHXOX_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR7005322.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            10461..11549
                     /locus_tag="ACHXOX_001843"
     CDS             10461..11549
                     /locus_tag="ACHXOX_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR7005323.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(11632..12929)
                     /gene="exuT"
                     /locus_tag="ACHXOX_001844"
                     /pseudo
     CDS             complement(11632..12929)
                     /gene="exuT"
                     /locus_tag="ACHXOX_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            13412..14824
                     /gene="uxaC"
                     /locus_tag="ACHXOX_001845"
     CDS             13412..14824
                     /gene="uxaC"
                     /locus_tag="ACHXOX_001845"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR7005324.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            14839..16326
                     /gene="uxaA"
                     /locus_tag="ACHXOX_001846"
     CDS             14839..16326
                     /gene="uxaA"
                     /locus_tag="ACHXOX_001846"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR7005325.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            16409..16960
                     /locus_tag="ACHXOX_001847"
     CDS             16409..16960
                     /locus_tag="ACHXOX_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR7005326.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(16965..18209)
                     /gene="sstT"
                     /locus_tag="ACHXOX_001848"
     CDS             complement(16965..18209)
                     /gene="sstT"
                     /locus_tag="ACHXOX_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR7005327.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(18825..19790)
                     /gene="alx"
                     /locus_tag="ACHXOX_001849"
     CDS             complement(18825..19790)
                     /gene="alx"
                     /locus_tag="ACHXOX_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR7005328.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(20073..21059)
                     /locus_tag="ACHXOX_001850"
     CDS             complement(20073..21059)
                     /locus_tag="ACHXOX_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7005329.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(21138..21821)
                     /locus_tag="ACHXOX_001851"
     CDS             complement(21138..21821)
                     /locus_tag="ACHXOX_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR7005330.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(21898..22401)
                     /locus_tag="ACHXOX_001852"
     CDS             complement(21898..22401)
                     /locus_tag="ACHXOX_001852"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR7005331.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            22486..23622
                     /gene="rlmG"
                     /locus_tag="ACHXOX_001853"
     CDS             22486..23622
                     /gene="rlmG"
                     /locus_tag="ACHXOX_001853"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR7005332.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            23907..24221
                     /gene="higB"
                     /locus_tag="ACHXOX_001854"
     CDS             23907..24221
                     /gene="higB"
                     /locus_tag="ACHXOX_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR7005333.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            24218..24634
                     /gene="higA"
                     /locus_tag="ACHXOX_001855"
     CDS             24218..24634
                     /gene="higA"
                     /locus_tag="ACHXOX_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR7005334.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(24679..26697)
                     /gene="fadH"
                     /locus_tag="ACHXOX_001856"
     CDS             complement(24679..26697)
                     /gene="fadH"
                     /locus_tag="ACHXOX_001856"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR7005335.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(26923..29274)
                     /gene="ygjK"
                     /locus_tag="ACHXOX_001857"
     CDS             complement(26923..29274)
                     /gene="ygjK"
                     /locus_tag="ACHXOX_001857"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR7005336.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(29291..30368)
                     /gene="ygjJ"
                     /locus_tag="ACHXOX_001858"
                     /pseudo
     CDS             complement(29291..30368)
                     /gene="ygjJ"
                     /locus_tag="ACHXOX_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(30502..>30824)
                     /locus_tag="ACHXOX_001859"
     CDS             complement(30502..>30824)
                     /locus_tag="ACHXOX_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005337.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         7817 a         7773 c         7795 g         7439 t
ORIGIN      
        1 acatggttat tttttatagg taatttatat aacgacattg aaatatttta aataaacatt
       61 agtaaattat tcattcatca acatgatgat aaaagccgga tgagtcagac atccggcgag
      121 cagatttgtt ttaaggttcc agttttggat caggtccaaa gcggttttcg cctggcgtac
      181 ccgcctggca gttaaagacg atgataataa gccagccgat aaacgggatt aagaatagca
      241 gtgcccacca cgccgagcgg tcggtgtcgt gcaggcggcg gaactgaact gcccaccacg
      301 gtaaaaacac cagaataccg tagattgtcg tcaggatgcc ttcgccacca gcgcgttgcc
      361 agcctaacat tttatccagc agccccagca cgaacgtaaa gatgatgttg accagaataa
      421 acatccagta ctctttgcgc cgcgcacgac cccggaaacc gacataattt tttattactt
      481 tcagatacca gtccattttt gcctcaattt tgctcaatca cttgttttta aagcagcaaa
      541 ttaagaatag acgtgaatga aatggcagta aatattcgtt caaggaatta ataaagtgct
      601 gtgaaggcgc gctaagactg atgacaagcg caaaatcgcc agatgcgcta cgcttatctg
      661 gcctgcgcgg tcattgcaac atattgaata taaatgtttt gtaggtttga taaggcgttc
      721 acgccgcatc cgatatgaac aacatgcact tagctaatta tcagaggcac ttcgtaaggt
      781 gccttttttt aaccgaagcg aacatctcgt cctgcggttc atcgagatcc tgccacggac
      841 caattgaaat aatccccgta gggttgatgg tcttatggct gcggaagtaa tgattacgga
      901 tgtgatcgaa attgacagtt tcggcgatcc ccggcatctg gtagatatcg cgcaggaagc
      961 catacagatt caggtaatcg ctgatgcggt gcttatcaca cttgaagtgg gtcacataca
     1021 ctggatcaaa acgcaccagc gtagtccaca ggcgaatatc ggcttcggtt agctggttgc
     1081 cggtcaggta acgatgctgg cctaaaatct gctccagtcg cgccagcgat tcaaacactt
     1141 tcgccaccgc ctcgtcgtag gcttgctggc tggtggcaaa accagcttta tacacgccgt
     1201 tgttaacggt gtcataaatc cagccgttaa gttcgtcaat tttcgtttgc agggctggtg
     1261 ggtagtaatc acccgctttc gcgcccagcg catcaaacgc ggtattgaac atgcggatga
     1321 tttctgttga ttcgttgctg acgatggtgt ggttcttttt gtcccacagt acgggaacag
     1381 taactcgtcc gctgtagtgt ggatcggcgt ggagataaag ctgatacaga aattcatgtt
     1441 gatagagcgt atctccggtt gctcccggaa aactgtcatc aaaggtccag ccgttttcca
     1501 gcatcagcgg gttcactacg gaaacggaaa taaacggttc cagtcctttg agtttgcgca
     1561 tgatcagcgt gcggtgcgcc cacgggcagg cgagtgaaat atagagatga taacgatctt
     1621 tctctgcgat aaaaccgcct gtgccagtgg ggccaggtgc gccatcggca gtgagccagt
     1681 tacgaaatgc ggaagctgaa cgttgaaatt taccgccggt agatttggta tcgtaccagg
     1741 tgtcatgcca gacgccgtca atcagttgac ccatttttct ctcctccaat agcaaaaacg
     1801 aggagcttcc tcctcgtttt ttatcgttaa gtatagcgcg tgcttaccac tttttattca
     1861 gcaggcggtc gatgctatac gcgcccggac cggtaattgc cagcagcagg aatccgccag
     1921 aaattgtcag gtttttcatg aacatcagcg agttgacgcc ttcagcaaag ttgctgtgaa
     1981 ataaaaatgc cgtcagcagc gtaaagcccg cagtaaacag ggctgtggtg cgagtcagga
     2041 aaccgaacag gattgccaga ccaccaccga actcaagcag aatcaccagc ggcagcataa
     2101 aacccgggac acccattgct tccatatatt gttgggtacc agagtaagcg gtgatttttc
     2161 cccagcctgc ggtaataaac agaatcggca ttaaaatgcg cgctaccagt acaccaacat
     2221 cttctaattt tttcatcata ctctccactg cgccactcag gctgctgatt gtttttttgt
     2281 gcaagggcgc ggtattagcg tcgttgctgt cgatggagag aatcataaac gtggtgaatg
     2341 atgattgtta gcaaggaaaa ctgtcaaaaa tcttcaaaaa atttgaggga taaggccgga
     2401 attgctccgg ccagagggaa gttaaccgcg aagctgttgc tgcttgaggg tcgttttaac
     2461 cagacgccag gcgctccata cgccaaaacc gcgtctggcc cagcggacca gcatattagg
     2521 atggcgaatc gtccagatcg ccatcacgct actgccaacc agcgcccagg agcgcagact
     2581 tagcagcata ttccagcgac gatcgtaagc gcctgttgcc tccagccatt cacgacgact
     2641 ggcggaaaga tccagccgtt gttgctggat ctggctaagc agttgcgcct tacgtcgttc
     2701 acgttcgact ttactgctca cgggactcct cctcgagcag ttgccgatcg tttgccagct
     2761 catggcgtgt atggcgcagc aaggtggatt tacgcgattt acgtaacgtc cagataccgc
     2821 caatcagtgc cagtagcagc aacaccacgg tggtggcaat catcgcattc aggcgatatt
     2881 gcggatcaac cgcccaaata attagcacca tcaggctcat aagaccaaat gcagcgaaaa
     2941 gcatcgtcag gcccagcatc agtaaaagtt gaaagagatt agctttttcc tcttccagct
     3001 ccaccaccgc cagccgcaga cgtgtttcca ccatttcaac catgatggaa acaattcgct
     3061 gcccgatgcc cagaacgctt ttaccgggcc cttgcgcgtg atgagtgtcc gccataatta
     3121 acgacgcgac agcagaacgc cgagcactac accgattgca gcgccaattc ccacgccggt
     3181 ccacggattt tcacgcacat actcatcagc acgcgccgcc gcgacacggg tttgtttggc
     3241 gatggcatca ccggtttcac ccaggcgata acggctctgt ttcagtgcct gctccgcttt
     3301 gctacgaatc ttactcaact cttctttcga cttctcgcca gatgagctaa gcacctcttc
     3361 cagcgtatcg gaaagggatt tcaactcagc acgcagatgt tccgtagtgt gttctttcga
     3421 catagttttc tccaggtgag taagtagtga ctattgtgag ttagtagtcg cgcgcttcca
     3481 gctttttcag ctcttcctgc gcttctgcca gtttccgttc gcgtttggca atcttatcgg
     3541 catcgccttt ttgcttcgcc tcggctaaat cttgctggcg ttccgccacc tcatctttct
     3601 gctttgcgat tttcttgtga tgatcggcac gcagctggct atctgaacag ttggcccgga
     3661 cttcactcag ggctttattc agaccgtcaa tacgattctg gttttggtgt ttttcggcat
     3721 agctgatctc cttaaggata ttttgctcct tttcctgaca cagggtagtg gcataactac
     3781 cggcactaag agcaaagaga gaaacagcta aagcgatgcg gtatttcatt cttgaacctt
     3841 ccattgtgat tcggacctat tacaaaggaa cgattgtcca gacaacgaag cggtagtgtt
     3901 aaccaccgcc tcattaatca tagacagtta cggtgaaatc accaaagtga gtgatatgtt
     3961 tcggataaca ggaagttatc cgaagcgatg agagttatcc cgtaaccggg tcagccactg
     4021 catagcctga ctgttattgt cctgctgcgc aatgatgtag ccatggggca atgttctgtc
     4081 gaggaccgct tttgcggcgg cgctctggtc gctggagtca aatgtaatta acagagtgtc
     4141 gtttttgggg gtgatacttt tgaaaggaat gccatgagcg tcaaggtgat gccagatcga
     4201 aaaaccgtct ggcatcgttg tgccttgatg aacggcacga atcgccagcg tagactcttg
     4261 ctggcgaacc gcggaccagg ccagcaacag cgtgccgacc agaagtaaaa cagtgccgga
     4321 ccaggctagc tggcgaagcg acatgcgagg tatttgcatc ccttaccccc gatttccata
     4381 tttctttttc cataacacga ccagagaacc tgccaggcca aacaccagca gcaccaccgg
     4441 gagcagcatc aggcatgaca tcagctggtc ctcgtacttt aaaaatatcg gcgttttgcc
     4501 gagcatgtaa cccagagttg tcaggatcaa tacccacagc agaccgctca tccagttgaa
     4561 aaactgaaag cgcgcgttat tcagccctga taacccggca atcgtcggca gcagtgttct
     4621 gacaaacgca ataaagcgac caattaacag cgccgataaa ccgtgtttat gaaaaagatg
     4681 gtgtgcgcgt tgatgataat gcgcgggtaa atgagatagc cagttttgta cggtgcgggt
     4741 attgcccagc catcgcccct gaatatagct gacccagcag ccgaggctgg cggcaacggt
     4801 cagcagcaga atcgtttgcg ggtaacccat cgcgcctttc gcaatcaaca cgccgaccaa
     4861 taccagtaaa ctgtcgcccg gtaaaaaggc cgctggaagc aagccgtttt caaggaacaa
     4921 aattacaaac aagacaaaat acaacatgcc aatcatcgat ggattggcca gggtttcaaa
     4981 atcctgcgcc cacagggctt gcagcaattg ggtcaaaagt tccattcatt attcctggtg
     5041 aatcggttaa cgccacgctg gttttaaaag atgcactacc gcaatgttat tgctgttttg
     5101 gtatgttctg cgggtttgat acatcaacac agcctgaact attccctgtt aaatcgtgag
     5161 ataagaaagc attcaaaaac ataaattcaa attgaatcta attgtaacaa aggtcaacgt
     5221 tgttcgttat caaaattaca tgtccgtgga gtctgatttt gctgaatgca ggggagggtg
     5281 gcgagaggat ttacacaggc tgacacttta tacttttcgc ttaccgacga agcgaacagg
     5341 cgcgtcgcag cgcctgtcgg acaggagttt catttactgc cactggtggc cgtatcgaga
     5401 tgcactaccg ggttttcggc gaatagatag cggtcggcat tgaactcgaa atcgtcgctg
     5461 gtttcgttaa ataacatgat tttggtgttt tccagatgct gccacattgc cagcttagca
     5521 gcatggggat ctttgcgaat taacgccttg agaatttgat cgtggtcatc acaccagtta
     5581 tcaacggtac gggaatcaat gtgttcgtgt agttttttcc agtacgggtt atggctacgc
     5641 tgggtccaca ttttttcaac gatggccgcc agggcggagt tctgcgtcgc cagggctacc
     5701 tgaatgtgga actgtaaatc ccactcggaa tcacggaagc attgttcgcc gcgcgcctgt
     5761 tcctgaatgg ccatcagttt catgatgtct tgtttcgtta cctgagttgc cgcgaactcg
     5821 gcaatattac tttcgatgag ctggcgagcc tgaagcaact caaacggacc gtaattggcg
     5881 aattccatat tattgtcagc cgcctgctga tggcgcggct ggttggaaac cacatgaata
     5941 cctgaacctt tacgcacctc cacatagcct tcaacttcca gcatgatgat ggcttcacga
     6001 actaccgttc ggctgacgtt cttttcatcg gcaataaagc gttcggcagg cagtttatca
     6061 cccaccagat agacgccctg ttcaatgcgc tctttcaggt cagcggcaag ttgttgatac
     6121 aaacggcgtg gttcagtgat ttccatatgc gtctcccgga gtggaatgcg gacgaagtta
     6181 tcataccact ttattcatgg gtatgccatt ggtcatatat aaatttggtg attgaattaa
     6241 tgtattgaat taatattgtg aatatttata aattcctaaa aaataagtat cgtcattgtg
     6301 aaagctacta aatttcagta agttaattcc cagcctttaa atttaatatt aactaaaaat
     6361 gcttttttat attgacttac aataaatcta catctctaat acaccaactt tttggaaaca
     6421 atattgtaag atcaaattta tatggtggcg aaaattaatc tcttatcacg gcttacgccg
     6481 cttttattcg tgttcgctcc ttttttggca cagtcaaata tgactgttta tcctatggct
     6541 gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag taatgaagtt
     6601 cagtacatca aggcgacagt attccgtatc gataatccat cgacgcctca ggaaaatgaa
     6661 gttgagatta aatccggtga tgcaaatcat ctggttgtta tgccacctaa attcgcttta
     6721 ccagccggta gtagcaaaac tgtgcgcttt gttgcaatgg aaccagagca aaaagaaaaa
     6781 aattatcgcg ttaaatttga agcggttccc agtattgatg acgttgccac agataaaaaa
     6841 gatctctcta tgcagttaac agttaactta atttggggga ttgttgttag tgttccacct
     6901 cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa aattagttaa tgcagggaat
     6961 caacgcttaa agattttaac gatcgcttat tgtaaaaata atagcaaaga aaattgtaag
     7021 atacagaccg taaataaaaa catcttcctt ggtcaggaaa gaaatcttga aagcatatct
     7081 ggctacgaca aaatcgttgt caaatataat aactggatca ccaaagataa tggcgagttt
     7141 gaactggcgg tccattaatt atcattgtaa taaacgaagg aatagtaatg aaaaaggtat
     7201 ttgcaaaatc tcttctggtc gcagcgatgt tttctgttgc aggctccgca ttggcagtac
     7261 aaaaggatat tactgtaacg gccaacgttg atgccgctct ggatatgaca cagaccgata
     7321 acaccgcgtt gccgaaagca gtagaaatgc aatatctgcc ggggcagggt ctgcaatctt
     7381 accagttgat gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa atgcagttag
     7441 tttccccggc gcaactggtt caaagcctgg atgctagcaa gattgttccg ctgacagtaa
     7501 cctggggcgg tgaagaaatt aaagctgatg cggcaaccac ttttaccgca accaaaatct
     7561 ttgcttccga tgcgttaact aacggttctc tggctaaaaa cctgatgttt gctcaaacca
     7621 ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt aagcatttat ctgtctcagg
     7681 atatctaatc cagataacag aattaacacg taacaaaggg aggggtagcc ctcccttatt
     7741 ttaactgttt tagtatttgt tatggatttc aggattgcca tggataaaaa attactggct
     7801 cttttgatcc tggcgagtct cagcccggca gaggcgacat taaccgaaat tcccgcaggg
     7861 tttgaggtta ttgctcaggg acagcaggag tatatcgagg tttatttttc agggaaaagt
     7921 ctcggtaaat attatgcaat ggttaatctt gataccgtaa catttcttga tccagcaagt
     7981 ctatataaca agctggaact ggatgtagac gatcagaaaa tcgcgcatat agtgaaagaa
     8041 aaattatcgc agccgctagc tcgccacggt gaattggctt gcggttatgt acgtactgac
     8101 tcagggtgtg gttttctgaa taccgatacg ctggaaataa tctataatga tgaagaaagt
     8161 tcggcaacgt tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa gtcattatat
     8221 ttaaatccag acaaaaatac tgttaatgct tttatacatc agcaagacat caatgttctg
     8281 gcacaggatg attaccaatc gttgtctatt cagggaaacg gtgcgctggg aataacagaa
     8341 aatagttata ttggtgcaca ctggaatttc aacggttatg atgcagatga tgtcagtgac
     8401 agtaatgctg acgtcagcga tctctattat cgttatgatt ttttacgtcg ttattatgtg
     8461 caggcgggtc gcatggacaa ccgcacacta tttaatgcac aaggcgggaa ctttaccttt
     8521 aactttctgc cactcggtgc aattgatggg atgcgtatcg ggtcgacact gagctattta
     8581 aaccaggcgc aaagccagca aggaacccca gtaatggttc tgctttcgcg caattctcgt
     8641 gttgacgctt atcgtaatga gcaacttttg ggatcgtttt atctcaatag tggttcgcaa
     8701 tttattgata ccagttcctt tccgccaggc agctacagcg tggcattaaa agtctacgaa
     8761 aataaccaac tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg tctgactgac
     8821 ggaaatgcgc aatggttctt acaggcaggt aaaactacat cacaggtttc tgatgatgaa
     8881 agttcagctt atcagctagg agtacgcctg ccattacatc cgcaatatga gctctacgca
     8941 gggctggcga atgccgatga tgtgagtgct ttcgagttag gtaataactg gacggcagat
     9001 ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc
     9061 aaaggtgata tgcaacaggc caactggagt aatccgggat ggccgacgtt gggcttttat
     9121 cggaccaact ctgacggcga tgcttgtaca accgacagca gagagagcta taacgcctta
     9181 agctgttatg aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg gaatatgatg
     9241 ctgggttata cccgcacaca aaataacact gatgatagtt tgcgttggga taaacagcag
     9301 agctttgaaa ataactatct tcgccagaca acagcgcaaa gtatctctga aactgtacaa
     9361 cttagcgctt cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt
     9421 ttccatcgta atgacaacgg tggcgataac gacgacaacg gcttgtactt atcgttttcg
     9481 ttatctgaca cgccaacgat ggatagcaat aacaacagcc attcaacaaa tgtgtctacg
     9541 gattatcgtt atagcgaaca ggatggcgat caaacgtcat ggcagttatc gcataccttt
     9601 tataacgatt cattcagcca taaagaactt ggcgtaaccg tcggaggcct gaacaccgat
     9661 accataaaca gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt ctacgctacc
     9721 gtatctgata gttatgaccg taagaatcat gatcatcttt cggcctttac gggcacttat
     9781 agctctacgc tggctgtcag ttgctatggc gttaatttgg gagccagtgg tacagacgat
     9841 ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg aacaggatga agagagtcag
     9901 gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac
     9961 agtgtgttgt tcccttatcc tggatttcag tctggttttg ttgaggttaa cgacagtagc
    10021 cagggcaatc agcaggggac aacaaacatc attaacggtg cagggaatcg tgaattaatg
    10081 ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta caactatatc
    10141 ggtcgtttat tactgccggc agcggtgaaa aaattcccga ttgtggggct gaacagcgcc
    10201 atgttactgg tagctgaaga tggaggattt acacttgaaa ttaacggcag cgaaaaagag
    10261 ctctatctgc tttccgggca gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc
    10321 gccagcattc gttacagcgg agatgttact tgtagtgtgg tgacttattc acaattaccg
    10381 gagtcgattc aggttcaggc acagttgaaa cagcctaaat tacgtggaaa cgttcagacg
    10441 gcgcaaaggg aggttgcacc atgagaaatc gattgattgc ggcgatattg ggcttgtttg
    10501 gcacgctcat tggcgttcag gcagctcctg acgtgaccag tgaaattacg tatgatttgg
    10561 catctggtag agcggattat tacttctgga aggatgaagc gtcggcagga aataatggat
    10621 atatgtggta tgaatgttcg tatcctgacc tccaacaaac ctgtacagct aatggaaata
    10681 tatcgacagt acaaatctat ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac
    10741 tcaaaggatt taaaacagcc attgtaagta gcgatgaagc gccgccagga tgcaaggggg
    10801 gcaaagggct tcagacgaat cttaaggatt ctaatagatc ttcatgtaca gaagatggtc
    10861 aacattatta tatatacgat acaaagtttc ttacgctcta ccttgagcag acagagatga
    10921 agaatttgcc gattggtggc gtctggaagg ggaaagttaa attacattcg aacagcccgg
    10981 cccaggacta tttcgcaaat attaccctga atacgctcga ccccaaccat attgacgtgt
    11041 tcttcccgga gttcgcccac gccacgccaa gggtgcagtt agacttgcat ccaacaggaa
    11101 gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg
    11161 gttttaacgg taatgccatc agttatgaaa tcatgctcaa agatgaaggg cgaccagctg
    11221 cagggcgcag agacggtgac ttctctatct atcgtcaagg agggaccacc accgacgagg
    11281 gagaacgcat tgattaccgg gtcaaaatgt acaacccgga aaccggtggg caaattgatg
    11341 tgcgcaataa tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg
    11401 tactgcccgg tattcgctat gccgtgatgt gtgtgccgac acccttgact ctggcagtcg
    11461 ataaattcag cgtgatggat aaacaggccg ggtactacat gggcaaattg tcagtaatct
    11521 ttacgccttc cttgccaacc atcaattgac cccgcagacc agaaaaaact aaacaacgaa
    11581 gccgcctgat aaggcggctt cgtcattaca acttacgtta aacgttaaac gttaatgttg
    11641 cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc gccggtttgt tctgcaatac
    11701 ggtccagata accagcgcac ccagcaggtc aaatactgcc agaactgcga acagcgggct
    11761 gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca aacagcgtac ttgccagcca
    11821 tgcggacatc ccggttaaac cgttcgccgt cgccacttcg ttacgaccga acacgtcaga
    11881 agagagcgta atcagcgcgc cagacagtgc ctggtgggca aaaccaccga tacacagcag
    11941 cataattgcg acatacgggt tggtgaacag accgatcata cccgggccaa tcatcagcac
    12001 agcacccagc gttacgacca tcttacggga aacgatcagg ttcacaccaa accaacgctg
    12061 gaacagcggc ggcaggtaac caccgaggat acaaccgagg tcagcaaaca gcatcggcat
    12121 ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg
    12181 gatccacgcg ttaaaagtac cccaggccgg ttctgccagg aaacgcggca gtgcgatacc
    12241 ccagaactga cggttacgca ggatctgacc aacggacatt ttcttcgccg tgctcacctg
    12301 gtgctgggct tcctgaccat taataatata gtcgcgttct tcatcggtca gatgcttctg
    12361 gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc cagataaagc tcaatgcacc
    12421 ggagatgata aatgccatct gccagctgtg catcacgatt gccataccac cagcggcggc
    12481 gcaatcatcg caccaatcga agaacctacg ttaaagtagc ctactgcgat ggaacgctct
    12541 ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc tgcttccgcg
    12601 gcaccgaccg caccacgagc aacagccagg ccaccccagc tacctgccag cgcggttaca
    12661 ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt acccagcaca
    12721 tccagtacat aacctgctac cggttgcatg accgtataag cagcagaata ggctgcgatg
    12781 atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg cgcagctgcc
    12841 gccacagtgt tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag tgcgatcata
    12901 taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt atatccgcag
    12961 gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa
    13021 aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc
    13081 ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg
    13141 atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag ccatcacaaa
    13201 tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg
    13261 cagcgagatt tatcactttg agagtaattt ttttaactac gtttattgat ctaactcacg
    13321 aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca
    13381 agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct
    13441 gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt
    13501 cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct
    13561 gtatgacatc tggctgaaag gcgatcacta caaatggcgc gctatgcgta ccaacggtgt
    13621 ggccgagcgt ctgtgtaccg gtgatgcatc tgaccgtgaa aaatttgacg cctgggcggc
    13681 gactgttccg cacactatcg gcaacccgtt ataccactgg acgcaccttg aactgcgtcg
    13741 tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga
    13801 atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa
    13861 cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat
    13921 cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt
    13981 caacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac
    14041 cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc
    14101 cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa
    14161 cgaagcggaa ctggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca
    14221 cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg
    14281 cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa
    14341 actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg cggaagagtt
    14401 gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctctactg
    14461 cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc agggcgaagg
    14521 tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat
    14581 ggaacgtcag atgacccaac tggcgcagct cggtctgctg agccgctttg tcggtatgct
    14641 gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg
    14701 ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acctgctggg
    14761 cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa
    14821 ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc
    14881 gctttagcag atttggctga aggcacagaa gtcagtgtcg ataaccagac tgttacgctg
    14941 cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa aggggccaac
    15001 gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac
    15061 gtccacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaacct
    15121 gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct
    15181 aacggcgatg tcggggtgcg taacgagctg tggatcctgc caaccgtggg ctgtgtcaac
    15241 ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc cgaaggtacc
    15301 gacggcgtgt tcctcttcag ccacacctac ggctgctcac aactgggcga cgatcacatc
    15361 aacacccgca ccatgctgca aaacatggtg cgccacccga acgcgggcgc agtgctggtg
    15421 attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt gggcgatatc
    15481 gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccggg
    15541 atcgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa
    15601 ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact
    15661 gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta
    15721 ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac
    15781 gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact ttaaacagta ctttattgcc
    15841 catgatcagc cgatctacga aaacccatca ccggggaaca aagcgggcgg tatcaccacg
    15901 ctggaagaca aatcacttgg ctgtacccag aaagcgggtt ccagtgtcgt ggttgacgtg
    15961 ctgcgttacg gcgagcgtct gaaaacgcct gggctgaact tgttaagtgc gccgggtaac
    16021 gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact
    16081 ggtcgcggca cgccatacgg tggatttgtg ccgacggtga aaatcgccac caacagtgaa
    16141 ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa
    16201 gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc caacggtaag
    16261 caaacttgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg
    16321 ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt
    16381 gcttttccat acctgataag gatttatcat gaccgcgtat tggctggccc agggcgtggg
    16441 tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa
    16501 aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta
    16561 tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct tacgcacgcg
    16621 cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt
    16681 ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt
    16741 ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat
    16801 tcacaacttc tgggcggggt cgataggcgg cacaatgatt gagggtagtt ttctgcttat
    16861 gaatggcttg aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa
    16921 agtagagaaa acccctcccg ccgtagacga aaggggttaa acaattaatt acgcagggcg
    16981 ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt
    17041 gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa gccgacggca
    17101 accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag cgggatcagc
    17161 agcagagaac cccccgccac gccggatgcg ccacaggcac acagagaagc caccacgctc
    17221 aacagcagcg ccgtgggcag atcgaccggg atacccagcg tattaaccgc agccagcgtc
    17281 aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggcacccag cggaatagaa
    17341 acggaatagg tatcgcgatc cagattcagc ttttcacaca gtgccatatt caccggaatg
    17401 ttcgccgcag agctgcgggt gaagaaggca tacacgccgc tttcgcgcag gcacagcagc
    17461 accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc
    17521 gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg
    17581 gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccaat cggtgcgaag
    17641 cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac caggtttttg
    17701 gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg
    17761 atgtagttac ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat taccagcccg
    17821 cgcatcactt ctacaatgcc tgacggtggc gaaatatcac ccgcgctact ggataagtgc
    17881 agggtagaag ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga gaaggtaccc
    17941 agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg gtggttagca
    18001 atagatgcca tcaccagcat caacaccagg atgggggcaa cggctttcag tgcgccgacg
    18061 aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgatatcca tgccagaaga
    18121 atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg
    18181 aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tatcgcacat
    18241 ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt
    18301 ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aagtcatatt
    18361 ttgaaactta atggttacat ttgtttgaca taaacagagg gaaggactgg ctactaaggc
    18421 tgattagata acatatgggc aaaactattt ggaatatgag aacggttgcg ctcctttaat
    18481 gtgctatgcc atataaagtc actggcgcag ggagcgcgca gggggcggcc aatcgccgcc
    18541 gccccctgca cccccaggct ctggcgaaca aaatcgccgc tgcgcgattc cctcagctta
    18601 tcccttgcgg ctaccgggtc gggcgcaagg gaacgtccct gtaagatgcg ccctcgccgc
    18661 gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg gcgattttga cgccaccaac
    18721 acccgtgcgg tctttttgta aagagctaca agccgattac ataattaata gctcaccttt
    18781 gtctggaatg agttatctct aggaatggct taatatacag cgaatcattc tccccgctgc
    18841 ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag aatgccaaac
    18901 accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag catcttgata
    18961 ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg ctctgctacg
    19021 cccgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt tgaggtcagc
    19081 acaatgaacg ggtcagtggt cacggcgaag atagccggaa tgctatccac ggcgaaaatc
    19141 acgtcgctca attccaccag aatcagtacc agcatcagcg gtgtggcgta cagcaatcca
    19201 ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt
    19261 agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc gagcgccatc
    19321 ttcacgccgg taaacagcag gaaggcaccg aagatataca ggatccagtc gaactgcgaa
    19381 attagccagc tgccagtgaa gatcatgatg gtacgcagaa caatcgcccc gagcacgcca
    19441 tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa caacatcagc
    19501 cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacccgt gagaaaggcc
    19561 agtgcctgtg gatcggcaac ggcgcgacct tcagtttgca ccagatacca ccagaaagcg
    19621 gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc ctgtttcatg
    19681 gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag cataatggcg
    19741 acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat agaagttcct
    19801 tacacataaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta tgcatagacc
    19861 tcgccttccg gcaaggtctc acttacaaca aaaaaggaat acgtctgcgt attcctttcg
    19921 ggttgcccgg tgaccggatg tggtttttca cacatcgtaa tgacgatcga ccggcaatga
    19981 agttactccc ctttgcaggt aacaaagtat gacagaccat tcagccagtc aatggtctgt
    20041 tacatacctt ttacactgtt ttactttggc aattatagtt ttacgctatc tgccggaaaa
    20101 atcaccccag tctggcggcg gatctcagtc agcagtttgg cggtgatgcg actgaccgcc
    20161 agccctggat gatccaccag atgctcatcc accagggtag caaacagctc tgcttcgtag
    20221 agcatggtat taatatgctg cggctgggtg agatcctgca tctggctgcc acgcggcacg
    20281 aagcacactt tctggcattc agacagtttt tcaatcacca gcgatcccgc ttcgccctga
    20341 atctcgcttt ccaggacaga atcactgacc tttgagtgct gcaacgtaac gctgaaatca
    20401 ccgtaatcca tcaccaccac gccgtgggca tctacgccgc ttgccaacaa actggcggtt
    20461 gcctgcacgc ttttcggttc accgaataat gccaccgccg acgccaggca gtaaaagccg
    20521 atatccataa ttgaaccgtt agagaatgcc ggattaaagg tgttgggatt ctcaccatcc
    20581 aggtaacgtt gataccgcga ggaatactgg caatagttga aaaagacttt acgcaatttg
    20641 ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg gcaggctggc ggttttaaat
    20701 gcctcaaaca gcaccacctg attttcccgc gcacaggcaa tggcggcatc cacttccgcc
    20761 agattcgacg ccagtggttt ctcgcaaatc acatgaattt tatggctaag gaaaagttgt
    20821 gtctgggaaa agtgcagggc attcgggctg gcaatataca ccgcgtcaat ggcatcgctt
    20881 tccgccatcg cttccagcga ggtaaacaga tgctcgacag aaaaatcatt ggcgaagtgc
    20941 tgggcctgtt caaggctgcg ggaatatacg gcggttaact tgtatttacc gctctcatgg
    21001 gcggcctcga cgaactggcg agtgatccag ttcgtaccaa tcacagcgaa acgtatcata
    21061 acgcgtggat actccataaa gggattctgt cgtcaatgta gcacgccttt ttcatcttcg
    21121 acgtgcgcag atacgcatta ttctttcgcc ggacagccat gctcactaaa cggcccaatc
    21181 atgaccgatg gcccgagata tttcggctgg cgatggagga ttttggcgtc aatcaccgcg
    21241 ctgacacgag cgagtttctc ccgcagttga gtataaaaac cgtgcttcat attaaggtct
    21301 ggcgcgttaa acccgatagc gtcgatgccg tactgttttg ccagccagat ggcgcgttgg
    21361 ttatgaaatt cttgcgagat aatggtgata tggttttccc caaacacctt tttggcacgc
    21421 accactgaat caagcgttga gaatccggca tagtcgcaga agatcacttt cgccggtacg
    21481 cctttagcga tcaaagcctg ctgcatacct gaggcttcat cgtaattttt acgtccgtta
    21541 tcgccactga ctaacagcca tttcactttt ccgacgtggt acaacgctgc tgcggtatca
    21601 atacgtcggg taaagtagcg attacccggc ctcgccccca gcaataaacc cacgttacgc
    21661 gccggaactg cgttgacatc gctccaggtc agctgcttgc tggcgttgac cattacgcga
    21721 tcggcgataa aaatggtcgc tcctgccaca agtaacagta agcaggtaat ctttagagtc
    21781 cggcgagaaa aacaaatacg gagaagaagg cggggctgca tggtcgttat ccttaacgaa
    21841 aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac atcattttca
    21901 gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga actcaagctg
    21961 gtggtactgc ggctccatat ggcagcacaa ctggtaaaac gctttgttat gctctttctc
    22021 cttcaggtgc gccagttcgt gcaccacgat catgcgtaaa aacggttccg gcgcgttgcg
    22081 aaacacagta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct gcacacgaga
    22141 aacagcggta tgtaatccga gcgcgttttt cagtacgtgg atcttattgt catacatcac
    22201 tttagtgatc ggcggggcat tgcgcagaaa ctgatttttt agatcctggg tatattgcca
    22261 gagggcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca gcacatcgcc
    22321 cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt agctgctcgg gataaccctg
    22381 aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta tactcacgca
    22441 cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag ccacttagac
    22501 aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt taacccgcta
    22561 caggcgtggg aagcggcgga tgaatatttg ctgcaacagt tggacgacac agaaatccgc
    22621 ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct ggcggaacat
    22681 aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga gaatttacgc
    22741 ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga ctacccgcaa
    22801 cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga acagcaactg
    22861 cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc caaagcccgt
    22921 gacattcaca cttccacgct ggagctgttc gagaaagtgc tcggcccgac caccactacg
    22981 ctggcatgga aaaaagcgcg cctgattaat tgcactttca atgaaccgcc gctggccgat
    23041 gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca caaccatgcg
    23101 aatgtcttct cccgcaccgg gctggatatt ggcgcacgct tctttatgca acatctgcca
    23161 gagaatctcg aaggtgagat tgtcgatctc ggttgcggta atggcgttat tggtctgacg
    23221 ctgcttgata aaaaccctca ggcgaaagtg gtgtttgtcg atgaatcgcc gatggcggtt
    23281 gcttccagcc gtttgaacgt tgaaaccaac atgccagagg cgttagatcg cagcgagttt
    23341 atgatcaaca acgcgctctc cggcgtggag cctttccgct ttaatgctgt gctctgcaac
    23401 ccgccgtttc accaacaaca tgcgctgacc gataacgtcg cctgggagat gttccaccat
    23461 gcccgccgct gcctgaaaat caacggcgag ctgtatattg ttgccaaccg tcacctggat
    23521 tacttccata aactgaagaa gattttcggc aactgcacca ccatcgccac gaataataaa
    23581 tttgtggtgc tgaaagcagt gaagctgggg cgtcgtcggt aagattggga gttacctgcc
    23641 ggatgcggcg ttaacgcctt atccggccta cgttcagcac gttactccca ggccggataa
    23701 gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc tggaatcgcg cttccataaa
    23761 acaaaaaatc cctctcacca tcctctacat tctcttgttt agcgtttttc tacgtttatt
    23821 cttccgtcac acagataaat tcatccgttg ccaatctgtc aacggatgtt atcatattgc
    23881 caatttgtca acggacgtga tgaagaatgc acctgataac tcaaaaagca ttgaaagatg
    23941 ctgcggaaaa atacccgcaa cataaaacgg agttggtggc tctggggaac acgattgcta
    24001 agggatattt caaaaaacct gagtcattaa aagcagtatt cccatctctg gataacttca
    24061 aatatctgga taagcattat gttttcaatg ttgggggcaa tgaattacgt gttgtagcaa
    24121 tggtcttttt tgaatcgcaa aagtgctaca tacgtgaagt tatgacgcat aaagaatacg
    24181 atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    24241 gcaagcagga gaaaagctaa ctgcggtggc accttttctg gcgggtattc agaacgagga
    24301 acaatacacc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    24361 cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    24421 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    24481 ggatcaatat ggtttaaccc ttgccgatct gccggaaatt ggcagtaaat ctatggtgtc
    24541 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    24601 attcggcatt tctcccgcct tgtttattga ttaacgcgac gtgccggatg cgttgcatcc
    24661 ggcgcttaaa ataacgattt aaatctccag cgccagccgt gttccctggg caattgcccg
    24721 tcgtgcgtcc agctccatag ccacatcgca gccgccaatt aaatgcacag ttttcccgct
    24781 atcaatcagc ggttgcgcca gcgcgcggtt tggctcttgc cctgcgcaga tcaccacatt
    24841 gtccactgct aatacctgcg tttcgccgtt gatcaccaca tgcagcccgt catcgtcaat
    24901 cttctgataa cttacgcctg ggatcatttt cacaccccgc gagagcaggg tggtgcgatg
    24961 gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc gctggagcat
    25021 cacaatttgc cgtgggctac gcgggatctg cattccctgc gggcttaagc caccagcctg
    25081 ttgtaggctg ctgtcgatcc cccactcatt acagaacctg gcgatattct ggctggtgga
    25141 ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac aaccgatgat
    25201 ggcaaccttg ttgcctaccg gcgctttgtc gcgcagtaca ttgagataac tcaatacctt
    25261 cggatgatcg atcccgtcga taggcggaac gcgcggcacg atcccactgg cgaggatcgt
    25321 ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttca gcgtcacgcc
    25381 cgtcacttcg atcatccggt ggtaatagcg cagcgtttcg taaaactcct ctttgccggg
    25441 gatctgtttg gcgatattaa actgcccgcc gatctcgctg tgggcatcaa acaatgttac
    25501 ctgatgccca cgcgccgccg cgttaatggc aaacgccagc ccagcaggtc ccgcaccgac
    25561 caccgccaga ttttttttct gcacggcagg aaggattggc attttggttt cgtggcaggc
    25621 gcgaggattc accaggcacg aggtgacttt gccaacgaag atctgatcga ggcaggcctg
    25681 attgcagcca atgcaggtgt tgatctcatc ggctcgcccc gattgcgctt ttgacagcag
    25741 ttccgcatca gcaagaaacg gtcgtgccat cgataccata tcggcatcgc cgcgcgagag
    25801 aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gttaccagcg gcagcgagac
    25861 gtggcctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg gcgtggcaat
    25921 ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg cgcccgccgc
    25981 ttcaatggcc tgcgccagct ctaccgtttc ggcaaaagtc ccgccgtctt cgaccaggtc
    26041 gagcatcgac agtcggtaga taataatgaa gtcgttgccg acgcgttcgc gcaccgcacg
    26101 cactacttct acggcaaatc gcatccggtt gcggtaatcg ccgccccact ggtcactacg
    26161 ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggacc ccattacctc
    26221 tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat cgtcgatcag
    26281 ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg gggcctgcaa
    26341 tgcggaggga gcgaccagat gcggttggta gctgtagcgc ccggtatgca aaatttgcag
    26401 ggctattttg ccgccttcct gatgtaccgc ttcggtaatg gtgcgatggt gtgggatctg
    26461 gctggcgtcg ttgagcattg cgccaccttc catgccaacg cctgttaaat ctggtgcaat
    26521 gccgccgctg acaatcagcg ccacgccgtg acgggcgcgt tcggcataaa acgctgccag
    26581 ccgctcggca ccgtccgggt attcctccag cccggtgtgc attgagccca tcaacacgcg
    26641 gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt agctcataaa
    26701 attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt agccgtcgat
    26761 ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga atacgcccgg
    26821 tggtaacgcc ggatgcaacg cttgccgcgt tttatccggc ctgggggatt gcacatgtag
    26881 ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattactgct tacggaaaaa
    26941 atcgttatac aacatataca aatgagcggc actccaggag aaatttggtg cgccttgctg
    27001 tgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc
    27061 gtgccggaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt aacgctccat
    27121 ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccacgccagt agatatcagc
    27181 gccaaaagcc ggattggtta acgccgccgt tcccagcggg acaaaggtgt tgaactcttt
    27241 aggatcgagc atcaccttca ccaccgcgtc ggcgttggcc tgcgttgccg caccgttaaa
    27301 cagcggcgac cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc
    27361 gttcgccagt ggtttatctt caatacgtac gtcatagtag aactgggtag tcgggtcgaa
    27421 catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc
    27481 ttctggctta ccgaggatgg ttgccatctc cgccagataa tggttatcgc tgtacatata
    27541 gctggcctga tccaccgact cctgcaatag cgagtagccc agcaacgttc cgtcctgact
    27601 gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccattag cgacatattt
    27661 atccagctgt tctttgtcga taaacccaaa gacggcggca tcatcacgcc ccgattccca
    27721 cgatgcagca acctgtgccg gaatttccag actgtcgtac aggcctttct ccaccacgcg
    27781 ggcgtagttg ttcagcccag actgcgtctc ttctttatcg ccttttttca ccgtaaacag
    27841 catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc
    27901 gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta
    27961 catctctgcc aaccaggctt tatcctgggt gacgttgtac acttccatca ccgaccaggc
    28021 ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac cacgctcggg
    28081 gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgctatc
    28141 gccaggctgg atctgccagg agaagaccgc gcggatattc tctttggcga tgtccggatt
    28201 gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct gattgccgga
    28261 gaaccagcgc ccggtcaccg acggtgtaac ggtgttaaat ttcaccgcac cgcccggtga
    28321 gagccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt
    28381 cgcatccgga ttggttaacc ctttcttcag gtattcttcc cagcgttgct gcgaggcggt
    28441 gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc
    28501 ctgagcggtc agcagatggg aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc
    28561 cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg
    28621 atattccgat tcgccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac
    28681 tttcaggcca tcacgggtgg cgctgatttt gcgctgatag tcagggtatt cgccagcaat
    28741 ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccgtc
    28801 ccacaccaga tccagcggtt tattgctggt gattttggtt tccagtagtg acgtgcgcgg
    28861 cgtggcgaag cgcagagtca tttcgacttg cacatctttt gctgtcagtt tttgcaccag
    28921 cgcaccggga atactgtatg cctccagcgt aaagtcgact ttcttgccgt cctgccagac
    28981 ggtcaggcgg tcgaaattgc tggccataaa gttgatgtac tcttccgtca gcagcgcaac
    29041 gcccggaaag ccgcccatgg tgttagggcc gtctggcaac agatgaccat gccaggctcc
    29101 gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg
    29161 cggcgcgcca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgggcgga
    29221 aaaacttatc agcagagcgc aggttactgg cgttaaaata gtttttattt tcatgaaatt
    29281 acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgtgag
    29341 aggcattttt catgtagaag cggctatcaa aagccgtggc gaaacgtccg ccgccgtatt
    29401 cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat
    29461 gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc
    29521 tcaccatcat cccactttca cttcccggta cgtccagccc gtcggcgcgt ccggtatggt
    29581 gccaggcgcg agcggcaaag ttaggagtta gcaggcccgt gagatagagc tgtcctgtgc
    29641 cgtcggtgat ttccagaccg ttcatccagg tgatgccttc ggcaaggtca tactggatgt
    29701 aaccgttcat cattgccgga taggtgtatt tgtcgtcata gcgatcgtat ttgccaaaat
    29761 gtagccaggc tttgctttca tcatgatgtc cggcaggagt cgcggtgacg ctataacgaa
    29821 agttgccgct taagttctgt accttcagcg cagtcataat gtcgcgggta ttgggaatga
    29881 cgtagcccag atcgggggtg aaatcgcccc accattgcag gtcatccagc gaggaatcct
    29941 gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt
    30001 taattccgcc ctcgaaacca ccccagctat ggtctggcac ccaggcattg ccgtcgtggt
    30061 cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct
    30121 ccaggccacc ttcaatatag gtaccgtcgc tgtatttatg tttattctca ccctctaaat
    30181 ccatatatcc accaccccct aatccctaat tcgccgtaaa aacgaaatga aggagaagat
    30241 gtcgaggtaa ttgcgggtgt ttttgtgtcg tcatgttctg ccggacgtgc ttcttctcct
    30301 gccgcagaaa aggcgtagca aaacggcaga gcaagtaatg ctctgcatgg tgcagtgatt
    30361 aatttcattg tgttaatcca tgagctaaag aaaaagtaca gccttttatc gttgtaatta
    30421 taaaaatata aaggcagaaa ctattttccc tctcctcatc caggaagagg gaaaatgcct
    30481 gctcattttt tattattctg cttaatcaac attgctggct ggcgtcgctt caatatggcg
    30541 ctcttcagcc gttaatccct ttatatattt actgtatttc caccacgcaa agccaaggaa
    30601 gataacaata ccgccgacgt tataaaaaat gatggtcaga atattcgcgc cagtcgggaa
    30661 tgtcgaagcg acaaacccta cggcaaatat cgcaatcagc attgaaacaa cgataatacc
    30721 ggtgcggcgg gagcccatac ggaaatcgcg tggcaggtga tctaatttgg cgcgtaaatt
    30781 caggtaagcc agcatgatga ataacggcgg aagcatggac gctg
//
LOCUS       DAZRVY010000040        30133 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZRVY010000040 DAZRVY010000000
VERSION     DAZRVY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.040"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..210
                     /gene="tuf"
                     /locus_tag="ACHXOX_001860"
     CDS             <1..210
                     /gene="tuf"
                     /locus_tag="ACHXOX_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR7005338.1"
                     /translation="GYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAM
                     DDGLRFAIREGGRTVGAGVVAKVLS"
     gene            440..823
                     /gene="secE"
                     /locus_tag="ACHXOX_001861"
     CDS             440..823
                     /gene="secE"
                     /locus_tag="ACHXOX_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR7005339.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            825..1370
                     /gene="nusG"
                     /locus_tag="ACHXOX_001862"
     CDS             825..1370
                     /gene="nusG"
                     /locus_tag="ACHXOX_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR7005340.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1529..1957
                     /gene="rplK"
                     /locus_tag="ACHXOX_001863"
     CDS             1529..1957
                     /gene="rplK"
                     /locus_tag="ACHXOX_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR7005341.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1961..2665
                     /gene="rplA"
                     /locus_tag="ACHXOX_001864"
     CDS             1961..2665
                     /gene="rplA"
                     /locus_tag="ACHXOX_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR7005342.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHXOX_001865"
     CDS             3078..3575
                     /gene="rplJ"
                     /locus_tag="ACHXOX_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR7005343.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3642..4007
                     /gene="rplL"
                     /locus_tag="ACHXOX_001866"
     CDS             3642..4007
                     /gene="rplL"
                     /locus_tag="ACHXOX_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR7005344.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHXOX_001867"
     CDS             4327..8355
                     /gene="rpoB"
                     /locus_tag="ACHXOX_001867"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR7005345.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHXOX_001868"
     CDS             8432..12655
                     /gene="rpoC"
                     /locus_tag="ACHXOX_001868"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR7005346.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHXOX_001869"
     CDS             complement(12897..14030)
                     /gene="thiH"
                     /locus_tag="ACHXOX_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR7005347.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHXOX_001870"
     CDS             complement(14027..14797)
                     /gene="thiG"
                     /locus_tag="ACHXOX_001870"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR7005348.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHXOX_001871"
     CDS             complement(14799..14999)
                     /gene="thiS"
                     /locus_tag="ACHXOX_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR7005349.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHXOX_001872"
     CDS             complement(14983..15738)
                     /gene="thiF"
                     /locus_tag="ACHXOX_001872"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR7005350.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHXOX_001873"
     CDS             complement(15731..16366)
                     /gene="thiE"
                     /locus_tag="ACHXOX_001873"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR7005351.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHXOX_001874"
     CDS             complement(16366..18261)
                     /gene="thiC"
                     /locus_tag="ACHXOX_001874"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR7005352.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18342..18442)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18495..18971)
                     /locus_tag="ACHXOX_001875"
     CDS             complement(18495..18971)
                     /locus_tag="ACHXOX_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR7005353.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19066..19839
                     /gene="nudC"
                     /locus_tag="ACHXOX_001876"
     CDS             19066..19839
                     /gene="nudC"
                     /locus_tag="ACHXOX_001876"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR7005354.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19879..20943
                     /gene="hemE"
                     /locus_tag="ACHXOX_001877"
     CDS             19879..20943
                     /gene="hemE"
                     /locus_tag="ACHXOX_001877"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR7005355.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYSLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20953..21624
                     /gene="nfi"
                     /locus_tag="ACHXOX_001878"
     CDS             20953..21624
                     /gene="nfi"
                     /locus_tag="ACHXOX_001878"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR7005356.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21667..22257
                     /locus_tag="ACHXOX_001879"
     CDS             21667..22257
                     /locus_tag="ACHXOX_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR7005357.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22444..22716
                     /gene="hupA"
                     /locus_tag="ACHXOX_001880"
     CDS             22444..22716
                     /gene="hupA"
                     /locus_tag="ACHXOX_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR7005358.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22729..23424
                     /locus_tag="ACHXOX_001881"
     CDS             22729..23424
                     /locus_tag="ACHXOX_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR7005359.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="ACHXOX_001882"
     CDS             complement(23426..23851)
                     /gene="zraP"
                     /locus_tag="ACHXOX_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR7005360.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24089..25465
                     /gene="zraS"
                     /locus_tag="ACHXOX_001883"
     CDS             24089..25465
                     /gene="zraS"
                     /locus_tag="ACHXOX_001883"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR7005361.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25462..26787
                     /gene="zraR"
                     /locus_tag="ACHXOX_001884"
     CDS             25462..26787
                     /gene="zraR"
                     /locus_tag="ACHXOX_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR7005362.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26784..28073)
                     /gene="purD"
                     /locus_tag="ACHXOX_001885"
     CDS             complement(26784..28073)
                     /gene="purD"
                     /locus_tag="ACHXOX_001885"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR7005363.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28085..29674)
                     /gene="purH"
                     /locus_tag="ACHXOX_001886"
     CDS             complement(28085..29674)
                     /gene="purH"
                     /locus_tag="ACHXOX_001886"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR7005364.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7052 a         7859 c         8305 g         6917 t
ORIGIN      
        1 ggctaccgtc cgcagttcta cttccgtact actgacgtga ctggtaccat cgaactgccg
       61 gaaggcgtag agatggtaat gccgggcgac aacatcaaaa tggttgttac cctgatccac
      121 ccgatcgcga tggacgacgg tctgcgtttc gcaatccgtg aaggcggccg taccgttggc
      181 gcgggcgttg tagcaaaagt tctgagctaa ttgccgataa catttgacgc aatgcgcact
      241 aaaagggcat catttgatgc cctttttgca cgctttcgta ccagaacctg gctcatcagt
      301 gattttcttt gtcataatca ttgctgagac aggctctgtt gagggcgtat aatccgaaaa
      361 gctaatacgc gtttcgattt ggtttgcctc gcgatcgcgg ggtgaaaatg tttgtagaaa
      421 acttctgaca ggttggttta tgagtgcgaa taccgaagct caaggaagcg ggcgcggcct
      481 ggaagcgatg aagtgggtcg ttgtggtggc attgctcctg gtggcgattg tcggcaacta
      541 cctttatcgc gacattatgc tgccgctgcg tgcgctggcc gtagtaattc tgattgctgc
      601 agcgggtggt gtcgcgctgt taacgacaaa aggtaaagct accgttgctt ttgcccgtga
      661 agcgcgtacc gaagtccgta aggtcatttg gccgactcgc caggaaacat tgcacaccac
      721 gctgattgtg gctgcggtta ccgcagtaat gtcactgatc ctgtggggac tggatggtat
      781 tctggttcgc ctggtatcct ttatcactgg cctgaggttc tgagatgtct gaagctccta
      841 aaaagcgctg gtacgtcgtt caggcgtttt ccggttttga aggccgcgta gcaacgtcgc
      901 tacgtgagca tatcaaatta cacaacatgg aagatttgtt tggtgaagtc atggtaccaa
      961 ccgaagaagt ggttgaaatc cgtggcggtc agcgtcgcaa aagcgaacgt aaattcttcc
     1021 ctggctacgt cctcgttcag atggtgatga acgacgcgag ctggcacttg gtgcgcagcg
     1081 taccgcgtgt gatgggcttc atcggcggta cttccgatcg tcctgcgcca atcagcgata
     1141 aagaagtcga tgcgattatg aaccgcctgc agcaggttgg tgataagccg cgtccgaaaa
     1201 cgctgtttga accgggtgaa atggtccgtg ttaatgatgg tccgttcgct gacttcaacg
     1261 gtgttgttga agaagtggat tacgagaaat ctcgtctgaa agtgtctgtt tctatcttcg
     1321 gtcgtgcgac cccggtagag ctggacttca gccaggttga aaaagcctaa cccagcgatc
     1381 aaaaaagcgg cgatttaatc gttgcacaag gcgtgagatt ggaatacaat ttcgcgcctt
     1441 ttgtttttat gggccttgcc cgtaaaacga ttttttatat cacggggagc ctctcagagg
     1501 cgttattacc caacttgagg aatttataat ggctaagaaa gtacaagcct atgtcaagct
     1561 gcaggttgca gctggtatgg ctaacccgag tccgccagta ggtccggctc tgggtcagca
     1621 gggcgtaaac atcatggaat tctgcaaagc gttcaacgca aaaactgatt ccatcgaaaa
     1681 aggtctgccg attccggtag taatcaccgt ttacgctgac cgttctttca ctttcgttac
     1741 caagactccg ccggcagcag ttctgctgaa aaaagcggct ggtatcaagt ctggttccgg
     1801 taagccgaac aaagacaaag tgggtaaaat ttcccgcgct cagctgcagg aaatcgcgca
     1861 gaccaaagct gccgacatga ctggtgccga cattgaagcg atgactcgct ccatcgaagg
     1921 tactgcacgt tccatgggcc tggtagtgga ggactaagaa atggctaaac tgaccaagcg
     1981 catgcgtgtt atccgcgaga aagttgatgc aaccaaacag tacgacatca acgaagctat
     2041 cgcactgctg aaagagctgg cgactgctaa attcgtagaa agcgtggacg tagctgttaa
     2101 cctcggcatc gacgctcgta aatctgacca gaacgtacgt ggtgcaactg tactgccgca
     2161 cggtactggc cgttccgttc gcgtagccgt atttacccaa ggtgcaaacg ctgaagctgc
     2221 taaagctgca ggcgcagaac tggtaggtat ggaagatctg gctgaccaga tcaagaaagg
     2281 cgaaatgaac tttgacgttg ttattgcttc tccggatgca atgcgcgttg ttggccagct
     2341 gggccaggtt ctgggtccgc gcggcctgat gccaaacccg aaagtgggta ctgtaacgcc
     2401 gaacgttgct gaagcggtta aaaacgctaa agctggccag gttcgttacc gtaacgacaa
     2461 aaacggcatc atccacacca ccatcggtaa agtggacttt gacgctgaca aactgaaaga
     2521 aaacctggaa gctctgctgg ttgcgctgaa aaaagcaaaa ccgactcagg cgaaaggcgt
     2581 gtacatcaag aaagttagca tctccaccac catgggtgca ggtgttgcag ttgaccaggc
     2641 tggcctgagc gcttctgtaa actaatgcct ttacgtgggc ggtgattttg tctacaatct
     2701 tacccccaca tataatgctt aatgcagacg tatatccgag atattcgggt tgtggcaagg
     2761 cggcaactga gtgagtcgcc aggagcatag ctaactatgt gactggtgcg aatgaaggaa
     2821 gccaacgccg ccacaagttg aatagcgacg gatagaaaag atttgttcgt tggagcctgg
     2881 cctatccagg cctccgtcga agaccgcagg agtttcgcaa gaaacttaat cccctgcgta
     2941 gacggtgaca gaacgctaag attattcttt tatattctgg cttgtttctg ctcaccgtaa
     3001 ttaagacgct ctctccgctt ggaggagtga agtgagttcc agagattttc tctggcaaac
     3061 atccaggagc aaagctaatg gctttaaatc ttcaagacaa acaagcgatt gttgctgaag
     3121 tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc ggattcccgt ggcgtaactg
     3181 tagataaaat gactgaactg cgtaaagcag gtcgcgaagc tggcgtatac atgcgtgttg
     3241 ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc gttcgagtgc ctgaaagacg
     3301 cgtttgttgg tccgaccctg attgcatact ctatggaaca cccgggcgct gctgctcgtc
     3361 tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt caaagccgct gcctttgaag
     3421 gtgagctgat cccggcgtct cagatcgacc gcctggcaac tctgccgacc tacgaagaag
     3481 caattgcacg cctgatggca accatgaaag aagcttcggc tggtaaactg gttcgtactc
     3541 tggctgctgt acgcgatgcg aaagaagctg cttaatcgca gttatctttt taacgcattc
     3601 gcttacgtat aaacttattc tgatattcag gaacaattta aatgtctatc actaaagatc
     3661 aaatcattga agcagttgca gctatgtctg taatggacgt tgtagaactg atctctgcaa
     3721 tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc tgtagctgct ggcccggttg
     3781 aagctgctga agaaaaaact gaattcgacg taattctgaa agctgctggc gctaacaaag
     3841 ttgctgttat caaagcagta cgtggcgcaa ctggcctggg tctgaaagaa gctaaagacc
     3901 tggtagaatc tgcaccggct gctctgaaag aaggcgtgag caaagacgac gcagaagcac
     3961 tgaaaaaatc cctggaagaa gctggcgctg aagttgaagt taaataagcc aacccttccg
     4021 gttgcagcct gagaaatcag gctgatggct ggtgactttt tggtcaccag cctttttgcg
     4081 ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg ctgtgccttt caatgcttgt
     4141 ttctatcgac gacttaatat actgcgacag gacgtccgtt ctgtgtaaat cgcaatgaaa
     4201 tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt ccattttccg gtcaacaaaa
     4261 tagtgttgca caaactgtcc gctcaatgga cagatgggtc gacttgtcag cgagctgagg
     4321 aaccctatgg tttactccta taccgagaaa aaacgtattc gtaaggattt tggtaaacgt
     4381 ccacaagttc tggatgtacc ttatctcctt tctatccagc ttgactcgtt tcagaaattt
     4441 atcgagcaag atcctgaagg gcagtatggt ctggaagctg ctttccgttc cgtattcccg
     4501 attcagagct acagcggtaa ttccgagctg caatacgtca gctaccgcct tggcgaaccg
     4561 gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct attccgcacc gctgcgcgtt
     4621 aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag gcaccgtaaa agacattaaa
     4681 gaacaagaag tctacatggg cgaaattccg ctcatgacag acaacggtac ctttgttatc
     4741 aacggtactg agcgtgttat cgtttcccag ctgcaccgta gtccgggcgt cttctttgac
     4801 tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt ataacgcgcg catcatccct
     4861 taccgtggtt cctggctgga cttcgaattc gatccgaagg acaacctgtt cgtacgtatc
     4921 gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg ccctgaacta caccacagag
     4981 cagatcctcg acctgttctt tgaaaaagtt atctttgaaa tccgtgataa caagctgcag
     5041 atggagctgg tgccggaacg cctgcgtggt gaaactgcat ctttcgacat cgaagctaac
     5101 ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc gccacattcg ccagctggaa
     5161 aaagacgacg tcaaactgat cgaagtcccg gttgagtaca tcgcaggtaa agtggttgct
     5221 aaagactata ttgatgagtc taccggcgag ctgatctgcg cagcgaacat ggagctgagc
     5281 ctggatctgt tggctaagct gagccagtct ggtcacaagc gtattgaaac gctgttcacc
     5341 aacgacctgg atcacggccc gtatatctct gaaaccttac gtgtcgaccc aactaacgac
     5401 cgtctgagcg cactggtaga aatctaccgc atgatgcgcc ctggcgagcc gccgactcgt
     5461 gaagcagctg aaagcctgtt cgagaacctg ttcttctccg aagaccgtta tgacctgtct
     5521 gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg aagaaatcga aggttccggt
     5581 atcctgagca aagacgacat cattgatgtt atgaaaaagc tcatcgatat ccgtaacggt
     5641 aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc gtatccgttc tgttggcgaa
     5701 atggcggaaa accagttccg cgttggcctg gtacgtgtag agcgtgcggt gaaagagcgt
     5761 ctgtctctgg gcgatctgga taccctgatg cctcaggaca tgatcaacgc caagccgatt
     5821 tccgcagcag tgaaagagtt cttcggttcc agccagctgt ctcagtttat ggaccagaac
     5881 aacccgctgt ctgagattac gcacaaacgt cgtatctccg cactcggccc gggtggtctg
     5941 acccgtgaac gtgcaggctt cgaagttcga gacgtacacc cgactcatta cggtcgcgta
     6001 tgtccaatcg aaacccctga aggtccgaac atcggtctga tcaactcttt gtccgtgtac
     6061 gcacagacta acgaatacgg cttccttgag actccgtatc gtaaggtgac cgacggtgtt
     6121 gtaactgacg aaattcacta cctttctgct atcgaagaag gcaactacgt tatcgcccag
     6181 gcgaactcca acctggatga agaaggccac ttcgtagaag acctggtaac ttgccgtagc
     6241 aaaggcgaat ccagcttgtt cagccgtgac caggttgact acatggacgt atccacccag
     6301 caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg aacacgatga cgccaaccgt
     6361 gcattgatgg gtgcgaacat gcaacgtcag gccgttccga ctctgcgcgc tgataagccg
     6421 ctggttggta ctggtatgga acgtgctgtt gccgttgact ccggtgtaac tgcggtagct
     6481 aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta tcgttatcaa agttaacgaa
     6541 gacgagatgt atccgggtga agcaggtatc gacatctaca acctgaccaa atacacccgt
     6601 tctaaccaga acacctgtat caaccagatg ccgtgtgtgt ctctgggtga accggttgaa
     6661 cgtggcgacg tgctggcaga cggtccgtcc accgacctcg gtgaactggc gcttggtcag
     6721 aacatgcgcg tagcgttcat gccgtggaat ggttacaact tcgaagactc catcctcgta
     6781 tccgagcgtg ttgttcagga agaccgtttc accaccatcc acattcagga actggcgtgt
     6841 gtgtcccgtg acaccaagct ggggccggaa gagatcaccg ctgacatccc gaacgtgggt
     6901 gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt acattggtgc ggaagtgacc
     6961 ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg aaactcagct gaccccagaa
     7021 gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg acgttaaaga ctcttctctg
     7081 cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc aggtctttac tcgcgatggc
     7141 gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc agctcaaaca ggcgaagaaa
     7201 gacctgtctg aagaactgca gatcctcgaa gcgggtttgt tcagccgtat ccgtgctgtg
     7261 ctggtagccg gtggcgttga agctgagaag ctcgacaaac tgccgcgcga tcgctggctg
     7321 gagctgggcc tgaccgacga agagaaacaa aatcagctgg aacagctggc tgagcagtat
     7381 gacgaactga aacacgagtt cgagaagaaa ctcgaagcga aacgccgcaa aatcacccag
     7441 ggcgacgatc tggcaccggg cgtgctgaag attgttaagg tgtatctggc ggttaaacgc
     7501 cgtatccagc ctggtgacaa gatggcaggt cgtcacggta acaagggtgt aatttctaag
     7561 atcaacccga tcgaagatat gccttacgat gaaaacggta cgccggtaga catcgtactg
     7621 aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga tcctcgaaac ccacctgggt
     7681 atggctgcga aaggtatcgg cgacaagatc aacgccatgc tgaaacagca gcaagaagtc
     7741 gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg gcgctgacgt tcgtcagaaa
     7801 gttgacctga gtaccttcag cgatgaagaa gttatgcgtc tggctgaaaa cctgcgcaaa
     7861 ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag aagcagaaat taaagagctg
     7921 ctgaaacttg gcgacctgcc gacttccggt cagatccgcc tgtacgatgg tcgcactggt
     7981 gaacagttcg agcgtccggt aaccgttggt tacatgtaca tgctgaaact gaaccacctg
     8041 gtcgacgaca agatgcacgc gcgttccacc ggttcttaca gcctggttac tcagcagccg
     8101 ctgggtggta aggcacagtt cggtggtcag cgtttcgggg agatggaagt gtgggcgctg
     8161 gaagcatacg gcgcagcata caccctgcag gaaatgctca ccgttaagtc tgatgacgtg
     8221 aacggtcgta ccaagatgta taaaaacatc gtggacggca accatcagat ggagccgggc
     8281 atgccagaat ccttcaacgt attgttgaaa gagattcgtt cgctgggtat caacatcgaa
     8341 ctggaagacg agtaattctc gctcaaacag gtcactgctg tcggggtaaa acccggcagc
     8401 ggattgtgct aactccgacg ggagcaaatc cgtgaaagat ttattaaagt ttctgaaagc
     8461 gcagactaaa accgaagagt ttgatgcgat caaaattgct ctggcttcgc cagacatgat
     8521 ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc atcaactacc gtacgttcaa
     8581 accagaacgt gacggccttt tctgcgcccg tatctttggg ccggtaaaag attacgagtg
     8641 cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc atctgtgaga agtgcggcgt
     8701 tgaagtgacc cagactaaag tacgccgtga gcgtatgggc cacatcgaac tggcttcccc
     8761 gactgcgcac atctggttcc tgaaatcgct gccgtcccgt atcggtctgc tgctcgatat
     8821 gccgctgcgc gatatcgaac gcgtactgta ctttgaatcc tatgtggtta tcgaaggcgg
     8881 tatgaccaac ctggaacgtc agcagatcct gactgaagag cagtatctgg acgcgctgga
     8941 agagttcggt gacgaattcg acgcgaagat gggtgcggaa gcaatccagg ctctgctgaa
     9001 gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa gagctgaacg aaaccaactc
     9061 cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg ctggaagcgt tcgttcagtc
     9121 tggtaacaaa ccagagtgga tgatcctgac cgttctgccg gtactgccgc cagatctgcg
     9181 tccactggtt ccgctggatg gtggtcgttt cgcgacttct gacctgaacg atctgtatcg
     9241 tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg gatctggctg cgccggacat
     9301 catcgtacgt aacgaaaaac gtatgctgca ggaagcggta gacgccctgc tggataacgg
     9361 tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct ctgaaatctt tggccgacat
     9421 gatcaaaggt aaacagggtc gtttccgtca gaacctgctc ggtaagcgtg ttgactactc
     9481 cggtcgttct gtaatcaccg taggtccata cctgcgtctg catcagtgcg gtctgccgaa
     9541 gaaaatggcg ctggagctgt tcaaaccgtt catctacggc aagctggaac tgcgtggtct
     9601 tgctaccacc attaaagctg cgaagaaaat ggttgagcgc gaagaagctg tcgtttggga
     9661 tatcctggac gaagttatcc gcgaacaccc ggtactgctg aaccgtgcac cgactctgca
     9721 ccgtctgggt atccaggcat ttgaaccggt actgatcgaa ggtaaagcta tccagctgca
     9781 cccgctggtt tgtgcggcat ataacgccga cttcgatggt gaccagatgg ctgttcacgt
     9841 accgctgacg ctggaagccc agctggaagc gcgtgcgctg atgatgtcta ccaacaacat
     9901 cctgtccccg gcgaacggcg aaccaatcat cgttccgtct caggacgttg ttctgggtct
     9961 gtactatatg acccgtgact gtgttaacgc caaaggcgaa ggcatggtgc tgactggccc
    10021 gaaagaagca gaacgtctgt atcgctctgg tctggcttct ctgcatgcgc gcgttaaagt
    10081 gcgtatcacc gagtatgaaa aagatgcaaa cggtgaatta gtagcgaaaa ccagcctgaa
    10141 agacacgact gttggccgtg ccattctgtg gatgattgta ccgaaaggtc tgccttactc
    10201 catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa atgctgaaca cctgctaccg
    10261 cattctcggt ctgaaaccga ccgttatttt tgcggaccag atcatgtaca ccggcttcgc
    10321 ctatgcagcg cgttctggtg catctgttgg tatcgatgac atggtcatcc cggagaagaa
    10381 acacgaaatc atctccgagg cagaagcaga agttgctgaa attcaggagc agttccagtc
    10441 tggtctggta actgcgggcg aacgctacaa caaagttatc gatatctggg ctgcggcgaa
    10501 cgatcgtgta tccaaagcga tgatggataa cctgcaaact gaaaccgtga ttaaccgtga
    10561 cggtcaggaa gagaagcagg tttccttcaa cagcatctac atgatggccg actccggtgc
    10621 gcgtggttct gcggcacaga ttcgtcagct tgctggtatg cgtggtctga tggcgaagcc
    10681 ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc cgtgaaggtc tgaacgtact
    10741 ccagtacttc atctccaccc acggtgctcg taaaggtctg gcggataccg cactgaaaac
    10801 tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg gcgcaggacc tggtggttac
    10861 cgaagacgat tgtggtaccc atgaaggtat catgatgact ccggttatcg agggtggtga
    10921 cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta actgctgaag acgttctgaa
    10981 gccgggtact gctgatatcc tcgttccgcg caacacgctg ctgcacgaac agtggtgtga
    11041 cctgctggaa gagaactctg tcgacgcggt aaaagtacgt tctgttgtat cttgtgacac
    11101 cgactttggt gtatgtgcgc actgctacgg tcgtgacctg gcgcgtggcc acatcatcaa
    11161 caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc ggtgaaccgg gtacacagct
    11221 gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt gcggctgctg aatccagcat
    11281 ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg aagtcggttg tgaactccag
    11341 cggtaaactg gttatcactt cccgtaacac cgaactgaaa ctgatcgacg aattcggtcg
    11401 taccaaagaa agctacaaag tgccttacgg tgcggtactg gcgaaaggcg atggcgaaca
    11461 ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac accatgccgg ttatcaccga
    11521 agtaagcggt tttgtacgct ttactgacat gatcgacggc cagaccatta ctcgtcagac
    11581 cgacgaactg accggtctgt cttcgctggt ggttctggat tccgcagaac gtaccgcagg
    11641 tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct cagggtaacg acgttctgat
    11701 cccaggtacc gatatgcctg cgcagtactt cctgccgggt aaagcgattg ttcagctgga
    11761 agatggcgta cagatcagct ctggtgacac cctggcgcgt attccgcagg aatccggcgg
    11821 taccaaggac atcaccggtg gtctgccgcg cgttgcggac ctgttcgaag ctcgtcgtcc
    11881 gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt tccttcggta aagaaaccaa
    11941 aggtaaacgt cgtctggtta tcaccccggt agacggtagc gatccgtacg aagagatgat
    12001 tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt gtagaacgtg gtgacgtaat
    12061 ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg cgtggtgttc atgctgtgac
    12121 tcgttacatc gttaacgaag tacaggacgt ataccgtctg cagggcgtta agattaacga
    12181 taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa gctaccatcg ttaacgcggg
    12241 cagctccgac ttcctggaag gcgaacaggt tgaatactct cgcgtcaaga tcgcaaaccg
    12301 cgaactggaa gcgaacggca aagtgggtgc aacttactcc cgcgatctgc tgggtatcac
    12361 caaagcgtct ctggcaaccg agtccttcat ctccgcggca tcgttccagg agaccactcg
    12421 cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa ctgcgcggcc tgaaagagaa
    12481 cgttatcgtg ggtcgtctga tcccggcagg taccggttac gcgtaccacc aggatcgtat
    12541 gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag gtgactgcag aagacgcatc
    12601 tgccagcctg gcagaactgc tgaacgcagg tctgggcggt tctgataacg agtaatcgtt
    12661 aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggtgt tattcagagg
    12721 gcaaattctt cacggaaaat attaagaaag gcagggcgaa acgaggaaga agcttttcgc
    12781 cctggacaac attcctgctg acggcactac cataaaaaaa cacaacgctt tcaacaacct
    12841 tgcgtttttc atcttccgta gcggctcgga ataaatttac gttcttacat tccgtctcat
    12901 agtctttgcg aggcgcgtcc cagatagctg tcccagtctt tccataccgg ctgcaaaccc
    12961 tgagcggtta acgcggcagc aaccgcttcc ggtctgcgat cgtcgtgcgg tgagaactgt
    13021 tccaactcgg ggtgattatc ggcatagcca cctggctgcg ttttcgagaa ggcgctgacg
    13081 ttattgatcg ccagcggaat aacgcgatcg cgaaaccacg gtgattcccg cgtggagagt
    13141 gacagttcaa tctccggtgc aagcagtcgg aaggcgcaga tggtttgcac taactggcgt
    13201 tcatccataa tcgacgcagg ctcaatgccg ccagtacacg ggcgcaggcg cggaaaggag
    13261 acagagtaac ggctttgcca gtaatgctgt tgcagccata gcaaatgttc ggcaaccata
    13321 tagcagtcaa cgcgccagtt gtcggaaagg ccaattagcg cgccgaggcc tatcttatca
    13381 atccccgcac gccccagccg atccggcgtt tccagccgcc agaagaagtc ctgttttttg
    13441 cctttcaaat gatggcgggt ataagtcgcc tcgtgatatg tctcctgata aaccatcacc
    13501 ccatccagac caagttgctt taactcggcg tattccgtct ccgccagcgg ttgcacttcc
    13561 atttgtagtg aagagaactg ttcacgcagg gcggggagat gacgacgaaa gtaatccatc
    13621 cccactttcg cctgatgttc accagtgact aatagcagat gttcaaagcc catctcccgt
    13681 atagcggcgc tttccctggc aatatccgct tcatccagcg ttttgcgctt gatgcgatta
    13741 ctcatggaaa atccgcagta cgtgcagtcg ttagcgcaaa gattggaaag ataaagcggg
    13801 acgtagaaac taactgtgtt gccaaatcgc tgacgggtca gacgctgcgc ccgttgggcc
    13861 agtggttcca gatagccact ggcggctggc gataacagcg ccatcatatc gtcgcgggtg
    13921 agttgcgagg catttagcgc ccgttctacg tcagcagccg ttttgccgtt gatacgcagg
    13981 cggatgtcgt cccagtccag ttgtcgccag cgatcgctga aggttttcat gccgatgcct
    14041 ccagaaatcc agtcagcggg ctggtggcat gagcaaaatg actgcggctg cccggtccgg
    14101 actgacgtgc cagcaggcct gcttctaccg ccagacgaaa tgccttcgcc atgttgacgg
    14161 gatcgtccgc tacggcaatc gccgtattca ctaacaccgc gtcggccccc atttccaggg
    14221 cctgcgcggc atggctggga acgccgatgc cagcatcgac aaccaccgga actgtggcct
    14281 gctggataat aatctccagc atggcacggg tttccagtcc ctgattcgag ccaatcggcg
    14341 cgccgagcgg catcaccgct gcacagccga cttcttccag acgtttacac aataccggat
    14401 cggccccgca gtaaggcagc acgacaaatc cctgttgtac cagcatttcg gcggctttca
    14461 gagtttcgat gggatcgggc aacagccagc gggcgtcagg gtgaatctct aattttaacc
    14521 agtttgtgcc taacgcttca cgtgccagat gggcggcgaa aatggcttct tccgctgttt
    14581 tcgccccgga agtatttggc agcagggtca cacccgccgc gataagcggt tcgaggatag
    14641 cgtcgttgtg ctggcgcaag tcgacacgtt tcatcgccag tgtcaccagc tggctgccgg
    14701 aagcgcggat cgcctccacc atcagttgtg aagaagcgaa tttccctgtg ccggtaaaca
    14761 gatgtgaatc aaacgttttg tccgcaatac gtaacatctc aaccccctgc aataacctga
    14821 aaaagcagga tctggtcgcc atcctgcacg atatgttgcg tccactgctc acgcgggacg
    14881 atttgctgat taatcgccag agccgcgccc gcttgtcgtt ggtcgagttg ctccagtagt
    14941 tcgtgaacag tttgcccggc ggcgcactgc atcggttgat cgttaaacag gatctgcatt
    15001 gcttcctccg cataccgggc aaccactggc gcggcgcaac gccaggctgc gccactggct
    15061 cgatttaccg tcgaacagtc ggagttctcc cgcaggtgtc tctataccgc ttaataactt
    15121 aatggcttcc agtgcctgca aagtgcccat aaccccgacc accgggccaa ccacgcccgc
    15181 cgtgcggcag ttgcgttctg gctcctggtt atctggccac aggcagcggt aacacccctg
    15241 ctcccagggc ggcgtcagta ccatcaactg accgccaaat ccgaccgcgc tggcggtgat
    15301 aagcggcgtg ttgagtacca cgcaggcggc attaatctcc tggcgagtcg ccatattgtc
    15361 ggtacagtcg agcaccacat cggcccgtgc aaccgcatct tttaacgcct cacccgttaa
    15421 ccgttgttgt aatgctgtca gttgaatatc gggattcaac tgtgtcagtc gctgatggct
    15481 gacctgcgat ttcgggcgat cgatatcttc agtggtaaag aggatttgtc gttgcagatt
    15541 gcttaaatgc acatcgtcgt catctgccag taccagcgtc ccgacgccag cccccgccag
    15601 gtatagcgca gcaggtgtac ccagcccgcc aagaccaata atcagcacct ggctgtcgag
    15661 cagtttttgc tgcccgtcca gagcgatatc gtcgagcaga atttggcggc tataacgcat
    15721 aaagtcacgg tcattcatcg ccaactcctg caatttccag caactgtgcc gttgccaaac
    15781 gccagtctgc ggcttgagta atggcgctga cgacggcgat actgccgaca cccgttgcta
    15841 tcaccgcagg cgcgcgcgcc agactgatac cgccaatcgc cacggtggga taatccgcca
    15901 gtcgctcaac atgccgtgcc agctgttcca gcccctgcgg tgcagaaggc atctgtttgg
    15961 tttgcgtcgg gaacacatgt cccagcgcga tataagaggg gcgtgctgcc agcgcgacgt
    16021 cgatttccat atcgtcatgt gtcgaaacgc ccagccgcag gcctgccgcg cggatggcat
    16081 tgagatcggt ggcttgcaaa tcttcctgcc ccaaatggac gccatacgcc tgatgcttga
    16141 tcgccagccg ccagtaatcg ttgataaaca atcgcgcgtt atagcggcgg cccagcgcaa
    16201 ttgccgccac gacatcggct tccacctctt cattgcgccg atctttgatg cgtagctgga
    16261 gagtacgtac gcctgcatcc aacagacgtt cgatccactg tacgctgtcc accaccgggt
    16321 acagtcctaa acgaaaaggt acaggaggaa aatcaggctg atacatcacg cttcctcctt
    16381 acgcaggtag atttctccgc ctctggcgcg gaagttctcc gacatatccg ccattcccac
    16441 ttcaatggtt tgcgcggcgg cgtaatcacg tacttcctgg ctgattttca tcgagcagaa
    16501 tttcggccca cacatggagc aaaaatgggc gactttacct gactcttgcg gcagggtttc
    16561 atcgtgataa gcgcgggcgg taaacgggtc gagggccaga ttaaactggt cttcccagcg
    16621 aaattcgaag cgggctttcg acatggcgtt atcgcgaatt tgcgcgcctg gatgcccttt
    16681 cgccaggtca gcggcgtggg cggcaatctt ataggtgata agcccctgct taacatcttc
    16741 tttattaggc agacccagat gctcttttgg cgttacgtaa cagagcatcg cgcagccaaa
    16801 ccagccaatc atcgccgcac caatccccga cgtgaagtgg tcatagcccg gcgcaatatc
    16861 ggtagttagc ggccccagag tgtaaaacgg cgcttcgtgg cagtgctcta actcctcggt
    16921 catattgcgg cggatcatct gcatcggcac gtggcctggg ccttcaatca tcacctgcac
    16981 gtcatattcc caggcaattt tggtcagttc gcccagcgta tgcagctcgg caaactgcgc
    17041 ttcatcgttg gcgtcctgaa tagaaccggg gcgcagaccg tcgcccagcg acagcgaaac
    17101 gtcataagcg gcacagattt cacaaatttc gcggaagtgt tgatagagga aattttcctg
    17161 atgatgggag aggcaccatt tcgccataat cgaaccgccg cgagagacga taccggtcag
    17221 gcgtttcgcg gtcatcggca catagcgcag cagtacgccc gcatggatag tgaagtaatc
    17281 cacaccttgc tcggcctgtt ccagcagcgt gtcgcggaac gcttcccagg taagatcttc
    17341 ggcgatcccg ttaaccttct ccagcgcctg gtagatcggc actgtaccga tcggcaccgg
    17401 gctgttacgc aaaatccact cgcgggtttc gtgaatatag cgaccggtgg agagatccat
    17461 caccgtatcc gctccccagc gcgtggacca taccagcttt tccacttctt cttcgatgga
    17521 agaggtgacc gccgagttgc cgatattggc gttaactttt accaggaaat tgcgaccaat
    17581 aatcatcggc tccgattccg gatgattaat gttggccggg ataatcgcac gtccggcagc
    17641 aacttcatca cggacaaatt ccgcagtgat attttccggc agatgtgcgc caaagctcat
    17701 tcccggatgc tggtggcgta aaacctcgct acggatgcgc tcgcggccca tattctcgcg
    17761 gatggcgatg aattccattt ccggcgtgat gatgccctgg cgggcgtagt gcagttgggt
    17821 gacacggcgt cctgttttgg cgcgttttgg cgttaataag ccgctaaaac gtagctcgtc
    17881 gagaccatca tctgccagcc gcgttctggt gtaatcggaa ctgcgcacgg taagttcttc
    17941 ggtatcgccg cgcgcatcga tccacggctg gcgtagtttt gccagccctt gctgcacgtt
    18001 aatggcgatc tgcggatcac catacgggcc ggaggtgtcg tagaccggaa tcgcttcgtt
    18061 ttcttcgtac tgcggctgtt ctttgctacc gccaattagc gtcgggctaa gctggatctc
    18121 acgcatcggc acgcgcacgc cggggtgtgt gccagtgata taaatgcgtt ttgagttggg
    18181 aaaggcggtg ccttccaggg tgtcgataaa atgttgggcc cgggcgcgtt gttcgcggcg
    18241 ggtcagtttt gttgcagaca tagctcattc caaaaagtca aggacgtggc ttgtcagacg
    18301 acggatgaag caagagacga tcgccgcaaa ggcgatgcga tagcagatta actcttgttc
    18361 ccttcgcagg tattagcctg atcaggttcc gcggatcccg aataaacggt ctcagccagt
    18421 taaggcactc cgacaagaaa ttagccccca caaacggggc gttgaatgta aattacgcgt
    18481 taacagcgca gaactcaagc aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc
    18541 agcttatctt ccagcacaaa gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt
    18601 tgaaactcaa ggtaattatc atgatcgatg gcggtttcca ggctggaatc gtagtaatcc
    18661 ataatctgtt gggtattggc ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc
    18721 ccgttgcctt ccagcttatg aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag
    18781 taatcgacca ggctctgaca aaaatcatca agggcttttt cgtttagcct catgtacgat
    18841 tctttgccaa gcttaatgcc aaccagattg tagtaagcca cgagcagatg cttacgtaca
    18901 tgtagccagc gatcaaccag tttgttactt cctctgacgc gctccgtcag gttatcgagc
    18961 tggttaagca tgattgactc cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa
    19021 aaaccatgtt aaacatgcca gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt
    19081 gaaaaattag atcacggctg gtgggtcgtc agccatgaac aaaaattatg gttgccgaag
    19141 ggagaattgc catatggcga agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa
    19201 atcggcgaat ggcaggggga acctgtttgg ttagtacaac agcagcggcg tcacgatatg
    19261 gggtcggtac gtcaggtcat tgatctcgat gttgggctgt ttcaactggc cggacgaggc
    19321 gtacaactgg cggagtttta ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg
    19381 tatccgagca aaaccgaatg ggcgatgctg tgcagccatt gccgtgagcg ttactacccg
    19441 caaatcgccc cctgcattat tgttgccatc cgtcgcgatg attcgatcct cctcgcccag
    19501 catacccgcc atcgtaacgg tgtccataca gtacttgccg gattcgtcga agtgggcgaa
    19561 accctcgagc aggcagtcgc gcgggaagtg atggaagaga gcggaattaa agttaaaaac
    19621 ttgcgttacg tgacttctca gccgtggccg tttcctcagt ctttaatgac cgcgtttatg
    19681 gcggaatatg acagcggcga catcgtgatc gacccgaaag aattgctcga ggcgaactgg
    19741 tatcgctatg acgatttgcc gttactcccg ccgcccggca ccgtagcgcg ccgtctgata
    19801 gaagatactg tggcgatgtg tcgggcagag tatgagtgat gatacactga ccgcctgacg
    19861 cactaaggaa cagccaaaat gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg
    19921 cgccagcccg ttgatgtcac tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg
    19981 gaatataaag ccacgcgcgc ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag
    20041 ctggcgtgcg aagtgacttt gcaaccgctg cgtcgctact cgctggatgc ggcgatcctc
    20101 ttttccgata tcctcaccgt gccggacgcg atggggttag ggctctattt tgaagccgga
    20161 gaaggtccgc gttttacctc gccagtcacc tgcaaagccg acgtcgataa actgccaatt
    20221 ccggacccgg aagatgagct gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa
    20281 ctgaaaggcg aagtgccgct gattggtttt tccggcagcc cgtggacgct ggcgacctac
    20341 atggtggaag gcggcagcag caaagcgttc accgtgatca aaaaaatgat gtatgccgat
    20401 ccgcaggcgc tgcacgctct actcgataaa ctggcgaaaa gcgtcacttt gtatctgaat
    20461 gcgcagatta aagccggtgc tcaggcagtg atgattttcg acacctgggg cggtgtgctt
    20521 accgggcgcg attatcaaca gttctcgctc tattacatgc ataaaattgt tgatggttta
    20581 ctgcgtgaaa acgacggtcg ccgcgtgcca gtcacgctgt ttaccaaagg cggcggacag
    20641 tggctggaag cgatggcaga aaccggttgc gatgcgttgg gcctcgactg gacaacggat
    20701 atcgccgatg cgcgccgccg tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg
    20761 tcgatgctgt acgcgccgcc tgcccgcatt gaagaagaag tagcgactat acttgcaggt
    20821 ttcggtcacg gcgaaggtca tgtctttaac cttggtcacg gcattcatca ggatgtgccg
    20881 ccagaacatg ctggcgtgtt cgtggaggca gtgcatcgac tgtctgaaca atatcaccgc
    20941 taaggagtga ttatggatct cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct
    21001 gtgatccgcg aggatcgact cgataaagat ccaccggatc tgatcgccgg agccgatgtc
    21061 gggtttgagc agggcggaga agtgacgcga gcggcgatgg tgctgctgaa atatccctcg
    21121 cttgagctgg tggagtataa agttgcccgc atcgccacca ccatgcctta cattccaggt
    21181 tttctttcct tccgcgaata tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag
    21241 ccggatttag tgtttgtcga tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc
    21301 agccattttg gtttgatggt ggatgtgcca acgatcggcg tggcgaaaaa acggctctgc
    21361 ggtaaattcg aaccgctctc cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc
    21421 gagcagctgg cctgggtctg gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc
    21481 ggccatcggg tcagcgtgga cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat
    21541 cgtctgccgg agccaacgcg ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg
    21601 cgctatacag caaatcagcc ctaattcagg taaactgcgg ataatttccg tatttgagaa
    21661 ctcaacatgt tacaaaaccc aattcatctg cgtctggagc gcctggaaag ctggcagcat
    21721 gtcactttca tggcttgctt atgcgaacgc atgtacccca attacgccat gttctgccag
    21781 caaaccggtt ttggtgatgg gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg
    21841 ctgaccgtta aagacgcaaa agtaaatttc gacagccaac tggagaaatt tgaagaagcg
    21901 attccttcag ccgacgattt cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg
    21961 gcgttaagtg aactggtcca ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa
    22021 gtgagtaaga cctccatcac gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa
    22081 atgagcgatg aagagcttaa agaaaaccca gctgtagagc aagaatggga cattcagtgg
    22141 gaaatattcc gacttttagc tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt
    22201 agggcagacc tgcgtgaggc gggtgagagc aatattggta taatttttca gcaataagac
    22261 cagaaaacgt gatttaacgc ctgatttgtc gtacctggag tcttcccttt cgccccccgt
    22321 ctggtctaca tttggggggc gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc
    22381 tattctggca tttccgtcgc actcgatgct tagcaagcga taaacacatt gtaaggataa
    22441 cttatgaaca agactcaact gattgatgta attgcagaga aagcagaact gtccaaaacc
    22501 caggctaaag ctgctctgga gtccactctg gctgcaatta ctgagtctct gaaagaaggc
    22561 gatgctgtac aactggttgg tttcggtacc ttcaaagtga accaccgcgc tgagcgtact
    22621 ggccgcaacc cgcagaccgg taaagaaatc aaaattgccg cagctaacgt accggcattt
    22681 gtttctggca aggcactgaa agacgcagtt aagtaagatt gcgtggcagt gaacagtttt
    22741 aacgaagggg tggtttcacc ccttttgtct ttctggcgtc gatcattgat gctggctggc
    22801 gctctgcttc tcactgcctg tagtcataac tcttcacttc ctccctttac cgccagtgga
    22861 tttgctgaag accagggcgc ggtacgcatc tggcgaaaag acagcggcga taatgtgcat
    22921 ctgcttgccg tgtttagccc gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg
    22981 cagggcgata acctcacgct catcaatatc aatgtttaca gcaaaccgcc ggtgaatatt
    23041 cgcgcgcgtt ttgacgatcg cggtgatctg agctttatgc aacgtgaatc cgatggggaa
    23101 aagcagcagc tttctaacga ccaaatcgat ttataccgtt atcgtgctga tcagatccgc
    23161 cagattagcg atgccttacg tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg
    23221 atggaacaga ccgtgaccac ctgcgaaggg caaaccatta aacctgattt agattcgcag
    23281 gcgatagcgc atatcgagcg ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg
    23341 ctggaagcgc ccgaaggttc gcaattactg ttagtggcaa actctgattt ctgtcgctgg
    23401 caacccaacg agaaaacgtt ctgatttacc agtggcccat acccatatga ccgccaccac
    23461 cgcagccgcc gtagcccatg cccattccgg ctccgcgcgg aatacccgct tcagccatcg
    23521 cgatatctcg tttcacccgt aactcatcta acgactgacg caaattctcc atctctttgg
    23581 cgaccgcgtt aattttgctg ctatccggtg ggttcgcggc taacagggca ttgtattcat
    23641 aacgcttcgt caccagttgc tgttgcagtg cgctgctttg agcgtaaaag tcattatgga
    23701 ttttctgcca cgctgtctgt tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac
    23761 cgtgtccgcc gtgagcaaat gcagatgtcg atcccatcgc cattgctgaa agcgccatca
    23821 ttaccagggc aattttcgtg ttccgtttca tggttaatcc tccagtggtt gtcttacttc
    23881 gggtattgca tcttccgtgc caacgatgaa acgctgatat gacgggtaat ctggcatgat
    23941 aaacgagtaa aaatgactcg cctgctgcgg gtagcgagtc atttttactc attgaaactt
    24001 aagcctttgt gttacagcgc agggtaagcg ctgataaaag atggcatgat ttctgctgtc
    24061 agaaagggat gagcaggcaa agaagaagat gcgttttatg caacgttcta aagactcctt
    24121 agctaaatgg ttaagcgcga tcctccccgt ggtcattgtt gggctggtag ggttgtttgc
    24181 ggtgacggtg attcgcgatt atgggcgcga gactgccgcc gccagacaaa cgctgctgga
    24241 aaaaggcagt gtacttattc gcgctcttga atccggctcg cgcgtcggca tggggatgcg
    24301 catgcatcat gcgcagcagc aggccttact ggaagaaatg gccgggcagc ctggagtacg
    24361 ttggtttgcg gtcacggatg aacaaggaac aatcgtgatg catagcaact ccggcatggt
    24421 gggaaaacag ctttattccc cgcaggaaat gcagcagtta catccgggag atgaagaagc
    24481 gtggcggcgg atcgatagcg cagacggcga gcctgttctg gaaatttatc gccagtttca
    24541 accgatgttt gctgctggaa tgcaccggat gcgccatatg cagcagtatg ccgcgacacc
    24601 acaagcaatt ttcatcgctt tcgacgccag taatattgtg agtgccgaag atcgtgagca
    24661 gagaaacacc ctgattatcc tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc
    24721 attcttctgg tatcgccgct atttgcgctc gcgccagctt ctacaagatg aaatgaagcg
    24781 caaagagaag ctggtggcgc tggggcatct tgcggcaggc gttgcccacg aaatccgtaa
    24841 cccactttcc tcgattaaag gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg
    24901 agaagcgcat caactggcgc aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt
    24961 aagcgagctg ctggaactgg ttaagccaac gcatctggct ttgcaggcgg tggatctcaa
    25021 cacgctgatt aaccactcat tacagctggt aagccaggat gcgaacagcc gggagatcca
    25081 gttacgcttt accgccaacg acacattacc ggaaattcag gccgatccgg acaggctgac
    25141 tcaggtcctg ttgaatctct atctcaatgc tattcaggcg attggtcagc atggcgtgat
    25201 tagcgtgacg gccagcgaaa gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa
    25261 gggaattgcg gcagatcagc ttgatgccat cttcactccg tacttcacca ctaaagccga
    25321 aggcaccgga ctggggctgg cggtcgtgca taatattgtt gaacaacacg gtggtacaat
    25381 tcaggtcgca agccaggagg gaaaaggcgc aacgttcacc ctctggcttc cggtcactat
    25441 tacgcgtaag ggcccacaag gatgacgcac gataatatcg atattctggt ggtggatgat
    25501 gacattagcc actgcactat tttgcaggct ttactgcgcg gctggggcta taacgtcgcg
    25561 ctggcgaaca gcgggcgaca ggcgctggag caggtgcggg aacaggtttt tgatcttgtg
    25621 ctttgcgatg tgcgaatggc ggagatggac ggcattgcca cgctaaaaga gatcaaagcg
    25681 ttaaatccgg cgattccggt gctgattatg accgcatact ccagcgtcga gacggcggta
    25741 gaagcgttga aaaccggggc gctggattat ctcatcaagc cgctggattt cgataacctg
    25801 caggcgacgc tggaaaaggc gctcgcgcat acgcacagta ttgacgctga aacgcctgcg
    25861 gtgtctgcca gccagttcgg tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt
    25921 gaaatcgccc tcgtcgcgcc atcggaagcc acggtgctga tccacggcga ttccggcacc
    25981 ggtaaagagc tggtcgccag ggcgattcac gccagtagcg cacgtagcga aaaaccgctg
    26041 gtaacgctca actgtgcggc actcaacgaa tccttgctgg aatctgaatt gttcggtcac
    26101 gaaaaagggg cgttcactgg agccgataga cgccgggaag ggcgctttgt tgaggcggac
    26161 ggcggcacgc tgtttctcga tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg
    26221 ctgcgtgcga ttcaggagcg cgaagttcag cgtgtcggta gcaaccagac tatctcggtt
    26281 gatgtccggt tgattgcggc gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt
    26341 tttcgccagg atctctacta tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg
    26401 caacggcggg aagatatccc tctgctggct gtccattttc ttcagcgctt tgccgagcgt
    26461 aatcgcaagg cggtaaaagg ctttacgcca caggcgatgg atttgctgat ccactacgac
    26521 tggccgggaa atattcgtga actggaaaac gcggtggaac gggcggtggt gctgctgacc
    26581 ggggaatata tttccgaacg cgagctgccg ctggcgattg ccggtacgcc gatcccactg
    26641 gggcaaagtc aggatattca gccgctggtg gaagtcgaaa aagaagtgat tctggcggca
    26701 ctggagaaaa cgggcggcaa caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa
    26761 acgctattgg caaaactgtc gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata
    26821 tctttccggc agaagcagtc gtcccagtga atatcggtca ttaaggcata agcgcgtttc
    26881 tgcgcttctg ccacggtatg acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc
    26941 actacctgct cgttatccgc cagtttcgtg cccgcgtgga acactttgcc gcctgccact
    27001 tcttccagcg gcaggccgtg gatcacatcg ccggtgcggt aatcgcccgg atatccaccc
    27061 gcagccatca ccacgccgag agaagcacgc tcatcccact ctgacgtttt ctcgtccagt
    27121 ttgccttcac aggccgccag gcagagctca accagatcgg acttcatgcg cagcataatc
    27181 ggctgggttt ctggatcgcc aaagcggcag ttaaattcga taaccttcgg attgccctgt
    27241 ttgtcgatca tcaggcccgc gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg
    27301 cctttcacgg ttggccagat gatacgttcc atggtgcgct gatgaacttc atcggttact
    27361 accggcgcgg gggagtaagc gcccatcccg cccgtgtttg gtccggtatc tttatcgcct
    27421 acgcgtttgt gatcctggct ggtggccatc ggcagcacat gctcgccgtc taccatcacg
    27481 ataaagctcg cttcttcgcc gtcgaggaac tcttcgataa cgatgcgatg acccgcgtcg
    27541 ccaaaagcgt tgcccgccag catatcgtga acagccgctt cggcttcttc cagcgtcatc
    27601 gccacgataa cgcctttccc ggctgccaga ccgtccgctt taatgacgat cggcgcacct
    27661 ttctcacgca gatacgccag cgcaggttct acctcggtga agttctggta ttccgctgta
    27721 gggatcttat ggcgggccag gaaatctttg gtaaacgctt tcgaaccttc cagttgcgcg
    27781 gcacctgcgg ttgggccgaa gattttcatc ccggcggcgc ggaaggtatc gaccacgcct
    27841 ttcaccagcg gcgcttccgg gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa
    27901 tccaacagcg ccgggatatc ggtcacgccg atagcaacgt tttgcagcgc gggttccagc
    27961 gcagtgcctg cattacccgg agcaacaaaa acagtttcaa ccagcggcga ctgcgccgct
    28021 ttccaggcca gcgcgtgctc gcgcccgccg ttaccaatca ctaatacttt catctattgc
    28081 tccattaatg gcggaagtgg cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg
    28141 cggcggcaat cacttcgtca tcacggatag aaccgccagg ctggattacg caggtcacgc
    28201 ctgcagcggc ggcggcatca ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg
    28261 aggaaccttt cacttccagg ccttcatcgg cagctttaat accggcgatt ttcgcggagt
    28321 acacgcggct catctggccc gcgccaatcc cgatggtcat attgtttttg gcatagacga
    28381 tagcgttgga tttcacgaac ttcgccactt tccagcagaa cagcgcatca cgcagttcct
    28441 gttcggtcgg ctgacgttgg gtgacgacgc gcagttcttc cgcaccgacc atgcccaggt
    28501 cacgatcctg aaccagcaga ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct
    28561 cgccccactg accgcaggtc agaacgcgta cgttctgctt ggcggcggtg attttcaggg
    28621 cttcttcgct ggcggaaggc gcaataatca cttcgacaaa ctgacgagaa atgatggcct
    28681 gtgcggtttc cgcatccagc tcgcggttaa aggcaatgat gccgccgaat gcggaggttg
    28741 ggtcggtttt gtacgcgcga tcgtaagcat caagaatgga attgccgata gccacgccgc
    28801 aagggttggc gtgcttcaca atcacacatg ccggctcggc gaactctttc acgcactcca
    28861 gcgccgcatc ggtatcggcg atgttgttat aagagagggc tttaccctga acctgggttg
    28921 cggtagcaac ggaggcttct ttcacattct cttctatata gaaggcagcc tgctggtggc
    28981 tgttctcgcc gtaacgcata tcctgcttct taatgaagtt caggttcagc gtgcgtggga
    29041 agcgaccggc ggcttctttg ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt
    29101 tggcaatcat gctgtcgtag gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac
    29161 gggttgcaag cgtcagcgat ccttcgttgt catccatctc tttaataatg gcgtcgtagt
    29221 cgctgctctt caccacgatt gcgacatctt tatggttctt agcggcggag cgcaccatgg
    29281 tcgggccgcc gatatcgatg ttctcaaccg catcttccag cgagcagcct tcacgagcaa
    29341 cagtctgggc gaacggatac aggttaacaa ccaccatatc gataggctgg atctgatgtt
    29401 cttccataat ggcatcgtcc tggccgcgac ggcccagaat gccaccatgt actttcggat
    29461 gcagggtctt cacgcgtcca tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg
    29521 ttaccggcag acctttttct gctaacagac gggcagtgcc ccctgtagac agcagctcca
    29581 caccgcgtgc ggaaagtgcc tgggcgaatt cgacgatacc ggctttgtca gaaacactga
    29641 gcagagcgcg gcggactgga cgacgttgtt gcatggtaaa tcccctggat ttgactatta
    29701 cagagagcgt tagctgaatt tttcgcgaaa aactcagcta acgcccctaa cggggcatcc
    29761 ttatttttcg cccgcattgt aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt
    29821 caatggtgat cacaattttg acagtggtta ccgtgggaaa aatacagaaa ttacattgat
    29881 gattgtggat aactctgtgc gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc
    29941 agtcagtcat ttttctgcaa tttttctatt gcggcctgcg gagaactccc tataatgcgc
    30001 ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg ttgaagagaa
    30061 aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    30121 cagtgcgcta aag
//
LOCUS       DAZRVY010000041        29548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZRVY010000041 DAZRVY010000000
VERSION     DAZRVY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.041"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHXOX_001887"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHXOX_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR7005365.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHXOX_001888"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHXOX_001888"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR7005366.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXOX_001889"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXOX_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR7005367.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHXOX_001890"
     CDS             complement(3644..4030)
                     /locus_tag="ACHXOX_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR7005368.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXOX_001891"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXOX_001891"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR7005369.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXOX_001892"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXOX_001892"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR7005370.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXOX_001893"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXOX_001893"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR7005371.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXOX_001894"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXOX_001894"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR7005372.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXOX_001895"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXOX_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR7005373.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXOX_001896"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXOX_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR7005374.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXOX_001897"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXOX_001897"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR7005375.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHXOX_001898"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHXOX_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR7005376.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXOX_001899"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXOX_001899"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR7005377.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXOX_001900"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXOX_001900"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR7005378.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXOX_001901"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXOX_001901"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR7005379.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXOX_001902"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXOX_001902"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR7005380.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXOX_001903"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXOX_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR7005381.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHXOX_001904"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHXOX_001904"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR7005382.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXOX_001905"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXOX_001905"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR7005383.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXOX_001906"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXOX_001906"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR7005384.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXOX_001907"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXOX_001907"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR7005385.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXOX_001908"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXOX_001908"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR7005386.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXOX_001909"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXOX_001909"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR7005387.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXOX_001910"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXOX_001910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR7005388.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHXOX_001911"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHXOX_001911"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR7005389.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZRVY010000042        29299 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZRVY010000042 DAZRVY010000000
VERSION     DAZRVY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.042"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(26..901)
                     /gene="nadA"
                     /locus_tag="ACHXOX_001912"
                     /pseudo
     CDS             complement(26..901)
                     /gene="nadA"
                     /locus_tag="ACHXOX_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
     gene            complement(1150..1281)
                     /locus_tag="ACHXOX_001913"
     ncRNA           complement(1150..1281)
                     /ncRNA_class="other"
                     /locus_tag="ACHXOX_001913"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1306..1381)
                     /locus_tag="ACHXOX_001914"
     tRNA            complement(1306..1381)
                     /locus_tag="ACHXOX_001914"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Lys,seq:ttt)
     gene            complement(1415..1490)
                     /locus_tag="ACHXOX_001915"
     tRNA            complement(1415..1490)
                     /locus_tag="ACHXOX_001915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1455..1457),aa:Lys,seq:ttt)
     gene            complement(1539..1614)
                     /locus_tag="ACHXOX_001916"
     tRNA            complement(1539..1614)
                     /locus_tag="ACHXOX_001916"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Lys,seq:ttt)
     gene            complement(1618..1693)
                     /locus_tag="ACHXOX_001917"
     tRNA            complement(1618..1693)
                     /locus_tag="ACHXOX_001917"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1658..1660),aa:Val,seq:tac)
     gene            complement(1843..1918)
                     /locus_tag="ACHXOX_001918"
     tRNA            complement(1843..1918)
                     /locus_tag="ACHXOX_001918"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1883..1885),aa:Lys,seq:ttt)
     gene            complement(2083..2874)
                     /gene="cpoB"
                     /locus_tag="ACHXOX_001919"
     CDS             complement(2083..2874)
                     /gene="cpoB"
                     /locus_tag="ACHXOX_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR7005390.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(2884..3405)
                     /gene="pal"
                     /locus_tag="ACHXOX_001920"
     CDS             complement(2884..3405)
                     /gene="pal"
                     /locus_tag="ACHXOX_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR7005391.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(3440..4732)
                     /gene="tolB"
                     /locus_tag="ACHXOX_001921"
     CDS             complement(3440..4732)
                     /gene="tolB"
                     /locus_tag="ACHXOX_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR7005392.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(4865..6097)
                     /gene="tolA"
                     /locus_tag="ACHXOX_001922"
     CDS             complement(4865..6097)
                     /gene="tolA"
                     /locus_tag="ACHXOX_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR7005393.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(6162..6590)
                     /gene="tolR"
                     /locus_tag="ACHXOX_001923"
     CDS             complement(6162..6590)
                     /gene="tolR"
                     /locus_tag="ACHXOX_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR7005394.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(6594..7286)
                     /gene="tolQ"
                     /locus_tag="ACHXOX_001924"
     CDS             complement(6594..7286)
                     /gene="tolQ"
                     /locus_tag="ACHXOX_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR7005395.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(7283..7687)
                     /gene="ybgC"
                     /locus_tag="ACHXOX_001925"
     CDS             complement(7283..7687)
                     /gene="ybgC"
                     /locus_tag="ACHXOX_001925"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR7005396.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(7837..8130)
                     /gene="ybgE"
                     /locus_tag="ACHXOX_001926"
     CDS             complement(7837..8130)
                     /gene="ybgE"
                     /locus_tag="ACHXOX_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR7005397.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(8130..8243)
                     /gene="cydX"
                     /locus_tag="ACHXOX_001927"
     CDS             complement(8130..8243)
                     /gene="cydX"
                     /locus_tag="ACHXOX_001927"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR7005398.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(8258..9397)
                     /gene="cydB"
                     /locus_tag="ACHXOX_001928"
     CDS             complement(8258..9397)
                     /gene="cydB"
                     /locus_tag="ACHXOX_001928"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR7005399.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(9413..10981)
                     /gene="cydA"
                     /locus_tag="ACHXOX_001929"
     CDS             complement(9413..10981)
                     /gene="cydA"
                     /locus_tag="ACHXOX_001929"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR7005400.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(11829..14462)
                     /gene="mngB"
                     /locus_tag="ACHXOX_001930"
     CDS             complement(11829..14462)
                     /gene="mngB"
                     /locus_tag="ACHXOX_001930"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR7005401.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(14480..16396)
                     /gene="mngA"
                     /locus_tag="ACHXOX_001931"
     CDS             complement(14480..16396)
                     /gene="mngA"
                     /locus_tag="ACHXOX_001931"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR7005402.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            16565..17287
                     /gene="mngR"
                     /locus_tag="ACHXOX_001932"
     CDS             16565..17287
                     /gene="mngR"
                     /locus_tag="ACHXOX_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR7005403.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(17391..18260)
                     /gene="sucD"
                     /locus_tag="ACHXOX_001933"
     CDS             complement(17391..18260)
                     /gene="sucD"
                     /locus_tag="ACHXOX_001933"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR7005404.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(18260..19426)
                     /gene="sucC"
                     /locus_tag="ACHXOX_001934"
     CDS             complement(18260..19426)
                     /gene="sucC"
                     /locus_tag="ACHXOX_001934"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR7005405.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(19520..20737)
                     /gene="odhB"
                     /locus_tag="ACHXOX_001935"
     CDS             complement(19520..20737)
                     /gene="odhB"
                     /locus_tag="ACHXOX_001935"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR7005406.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(20752..23553)
                     /gene="sucA"
                     /locus_tag="ACHXOX_001936"
     CDS             complement(20752..23553)
                     /gene="sucA"
                     /locus_tag="ACHXOX_001936"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR7005407.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(23854..24570)
                     /gene="sdhB"
                     /locus_tag="ACHXOX_001937"
     CDS             complement(23854..24570)
                     /gene="sdhB"
                     /locus_tag="ACHXOX_001937"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR7005408.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(24586..26352)
                     /gene="sdhA"
                     /locus_tag="ACHXOX_001938"
     CDS             complement(24586..26352)
                     /gene="sdhA"
                     /locus_tag="ACHXOX_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR7005409.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(26352..26699)
                     /gene="sdhD"
                     /locus_tag="ACHXOX_001939"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(26352..26699)
                     /gene="sdhD"
                     /locus_tag="ACHXOX_001939"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR7005410.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(26693..27097)
                     /gene="sdhC"
                     /locus_tag="ACHXOX_001940"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(26693..27097)
                     /gene="sdhC"
                     /locus_tag="ACHXOX_001940"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR7005411.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            27478..27585
                     /locus_tag="ACHXOX_001941"
     CDS             27478..27585
                     /locus_tag="ACHXOX_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005412.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            27791..29074
                     /gene="gltA"
                     /locus_tag="ACHXOX_001942"
     CDS             27791..29074
                     /gene="gltA"
                     /locus_tag="ACHXOX_001942"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR7005413.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         7100 a         7885 c         7140 g         7174 t
ORIGIN      
        1 tcttatatca ctggcgctga cacctgcggc aggttgcacc ctcacctgcg gttggtgctt
       61 ccagtaactc tttatctggc accgcctgct gcattttgta gaaaatgccc cgatcggttg
      121 ccacgataag ccgctgatgt ggcaatgttt tcgcagcagc gatcagttga ctggtggaac
      181 cgaccgcatc cgccatatcg acaatagctt gtggtgattc tggatgcacc agtatggcag
      241 catccgggta ttcttcttgc aagcgggtta acgcctgagt cttaaattca tcatgcacaa
      301 tacaggcacc ctgccagcat aaaatgtctg cgcccgtctg tttttgcacg taacgcccca
      361 gatgtttgtc gggtgcccag atgatttttt caccaaaact atccagatga tcaataagtt
      421 cgacggcaat gcttgaagtt accacccaat ctgcgcgcgc ttttaccgca gcagaagtgt
      481 tggcgtagac gacgacagta tgatcgggat gggcatcgca aaatgcgtta aattcttcaa
      541 cagggcagcc gagatccagt gaacattcag cctgaagtgt cagcatcaga attgtttttt
      601 ccggactgag aattttggcg gtttctccca taaatctcac cccagcgact aacaacgtag
      661 aagcgggatg ctttgcaccg aagcgcgcca tttccagaga atcagaaata cagccaccgg
      721 tttcttctgc cagttgttga atttcggggt cggtatagta gtgggcaacc atcaccgcat
      781 tacgttcttt tagcagacgt tttatcttct cgcggtaata cgctttttca tcaatgctta
      841 acggcgtcgg cttcgggggg aaaggataaa tcgccgtgtc tggatcaaac atcacgctca
      901 tcttaccatc tcgttttaca ggcttaacgt taaaaccgac attagcgtaa tattcgctac
      961 tatcggcgta atttgtttta tatgctaaac aagatagtcg aatggtgggg aaaagtcact
     1021 cgaattacgc ctaattccac cgacgtagag ttgcttgcac agtaagctct gacgatgtca
     1081 tcaggtttac aaagtgaaat tgaggatgcc tgaatagcaa aaaagcgcct ttagggcgct
     1141 tttttacatt ggtgggtcgt gcaggatgac tcgcttcgct cgcccttcgg gccgtcgccg
     1201 cgagcggctc cgttgtttcg ctgcgctcaa ctcgaacctg ctgcaggttc gaatctttga
     1261 atatctcaga tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag
     1321 gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc
     1381 ccttgcggga ttatgctgct ttaatcattc aggatggtgg gtcgtgcagg attcgaacct
     1441 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
     1501 tacctgaaag atttctactc ggggccaatc tttaagattg gtgggtcgtg caggattcga
     1561 acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg acccgagtgg
     1621 tgggtgatga cgggatcgaa ccgccgaccc cctccttgta agggaggtgc tctcccagct
     1681 gagctaatca cccgatactg cgctggatac tacaaactac ttccaccgaa cccaccgtaa
     1741 ttagtggtgg gtcgtgcagg atgactcgct tcgctcgccc tacgggccgt tgctgacgca
     1801 acgttatcct acacgcttaa catctgtctt cgatgctaaa actggtgggt cgtgcaggat
     1861 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac
     1921 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
     1981 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
     2041 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
     2101 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
     2161 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
     2221 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
     2281 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
     2341 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
     2401 gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct
     2461 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
     2521 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
     2581 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
     2641 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
     2701 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
     2761 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
     2821 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
     2881 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
     2941 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
     3001 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
     3061 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     3121 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     3181 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     3241 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     3301 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     3361 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     3421 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     3481 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     3541 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     3601 catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     3661 tgtgctgctg cccaccgttg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     3721 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     3781 tcactttata aacctgtgga cgaccggcct ggtcagaagt aaatgccagg ttctggctgt
     3841 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     3901 cagaagccaa atccattacg tacaggttca ggctaccggt tttcgacaag gcgaatgcca
     3961 gtttgctgcc gtctggcgag aatgcaggcg caccgttgtg acgcgggaat gaagccacct
     4021 gacgtacagc gccgtttgcc agcgtttgga taactagcgc ggaacgaccg ctttcgaagg
     4081 tcacataagc cagtttagaa ccgtctggtg accacgccgg ggacatcagc ggctgtggtg
     4141 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     4201 actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc
     4261 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcatagcgc agccactgct
     4321 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     4381 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
     4441 cgtcaatacc cagcgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     4501 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcggcagcaa
     4561 cgatgccgcc aatatcttca ggtgccgcac cagggcccgc ccactggaaa ggaacaacac
     4621 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     4681 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     4741 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     4801 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtcggaac agtcaacatc
     4861 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     4921 gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
     4981 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     5041 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     5101 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     5161 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     5221 cagagcttag ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt
     5281 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     5341 cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct ttcttatcag
     5401 ctgctgcctt atcagcagcc gctttttctg ctgctttttt gtcggctgca gctttctctg
     5461 ctgccgcttt ctttcttgct tcagctgcag cagcttctgc cgcttccgct ttcttcttca
     5521 gtgccgcagc ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg
     5581 cttctgcttt tttcttcgcg tctgccgccg ctttcttcgc tgcttcttcc gcagctttag
     5641 catccgcttc ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc tcttccgctt
     5701 gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgttcctgag
     5761 cagctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac
     5821 gcagttcttc agcagcctgc tgttccttta tcttgcgctg ctcatcagaa cgcttcgcgc
     5881 ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga
     5941 cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg
     6001 aactccagag cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc
     6061 gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg
     6121 cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt
     6181 aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg
     6241 taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg
     6301 ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc
     6361 accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg
     6421 ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg
     6481 ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg
     6541 ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc
     6601 ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata
     6661 aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgaggcggtt ataagccatt
     6721 accgccggaa ttgcggcgaa cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg
     6781 ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg
     6841 atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg
     6901 aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc
     6961 gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac
     7021 tctttgaacc cgctgtaaaa tatttgttcc gaaccagtca gattatcccg tttcccctga
     7081 ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg
     7141 gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaggatgcg
     7201 attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac
     7261 aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg
     7321 cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca
     7381 aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg
     7441 ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg
     7501 tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat
     7561 gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca
     7621 ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg
     7681 tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata
     7741 cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc
     7801 tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc
     7861 gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg
     7921 cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc
     7981 atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca
     8041 tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac
     8101 cgcatacaag gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat
     8161 tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc
     8221 agaatccatg cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac
     8281 gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccaa gcggtgtaga
     8341 gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc
     8401 tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa
     8461 acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca
     8521 caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc ggaagaacca
     8581 cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc
     8641 cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg
     8701 atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga
     8761 ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca
     8821 cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc
     8881 ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca
     8941 gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta
     9001 ccagcggcgg aacgaaacta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg
     9061 tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca
     9121 cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag
     9181 caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa
     9241 tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc
     9301 ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca
     9361 gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg
     9421 ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg
     9481 ggccgaggcg tgcaaacttg aacattaaga acaattctgc caccaggaac agggtataca
     9541 ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gagttcgcca
     9601 cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc
     9661 agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagaagcc
     9721 attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca
     9781 gaagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc gctacacgcg
     9841 ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg
     9901 tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac
     9961 gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc
    10021 cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat
    10081 ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg
    10141 tctcctcttc ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt
    10201 cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg
    10261 attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag
    10321 caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac
    10381 cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct
    10441 gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa
    10501 agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg
    10561 cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca
    10621 gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca
    10681 gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc
    10741 agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga
    10801 taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga
    10861 cgtagaccgt ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa
    10921 ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca
    10981 tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac
    11041 acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa
    11101 aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata
    11161 tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc
    11221 gacggtgatc aatttatagc taaattaccg cctttcagcc aatttgatcg agaacaattt
    11281 atctcttttt gatgccaatt tccaagactt atacattgat aaatatcaat tcctacaaat
    11341 tatgttaatc atgtttcaat aaggtaactt tagtgacatt tatgtttaaa atgtgtgagt
    11401 tataaggctg aaattagttt cggttggcaa tttaaagata aaaagagacg aagcaaaaac
    11461 agcacggttg accttaattc tttttttggt cactggctaa tacgacgcag agtttcagga
    11521 ttactaataa aggcataaat ccaccccgcc gatattaaca tttaattaac ttgtaattat
    11581 attataatta actcacccag ggttattatt ttgttaaaaa taaagccaca tatacgtggc
    11641 ttgagagtga agataaacgc aaaattacct gatgcgctac gcttgccagt gtctacatca
    11701 tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt catgccgtat
    11761 ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg cttcggtttg
    11821 attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa aaaaggcccc
    11881 gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat cgtttctgag
    11941 caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga ttcagccgga
    12001 ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc gcttatcagg
    12061 catcccactg ggggcatttt caacaaacta taactttccg gcacgttgaa tccggctttg
    12121 ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt cagccatgct
    12181 cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa taaacttagg
    12241 cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat tcccgaaggc
    12301 cgcccaggcc ttaaaagcag atcttctttg cccagtaagc ccacgccacg cagcaacgta
    12361 atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtaaccc ttcgctaaag
    12421 acagccatgc cgttacgccc ttcctgtaag gcaacatagt tgagcatatt ccataccgga
    12481 accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc gttcacgggg
    12541 cgcgttagcg aaccaaactg cgtatctgcc agaacactgt cagtgttaaa aggtgtaggg
    12601 accaggacac gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc cacatcaata
    12661 cgcctgctgt tatgactaag agtgacaacc aacacccccc ctactctgcc agtggattgc
    12721 cgggcgctgc gttctgacaa attgagcggc actgccatgt catagcggat aacagccctg
    12781 ctctgccagg cttcatgaat aatatcgcat tgcggtttcg cgttcgctgc ggtaattacc
    12841 cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct ttcttcaatt
    12901 tgcaataccc ggtcatagcg cacaccgctg tctttatcta ccagttgcag agaaccatcc
    12961 tcattgagcg caatctgcca gaaagcattt tccagaatcc cttcagcgtc actttttgcc
    13021 gcaatcacgt tgccaggctg attcgcttca atataaagcg tgcgatagcc catagaaggg
    13081 acaatctggt tgatctgaat atcaaactcc ataaagggat cataattgcc ataatgaaca
    13141 atttgccgat cgattaggcc aggatcgatc tcacgggcat ggcgaataaa atacggtaca
    13201 ggctgaccgc gatcgtcttg tagattaaac tggctggcgc gcagccgcac agtggtgtta
    13261 ataacttctt cccgcggcca gggcatcagg ttaaacagca cgagtttgtc ggcatcactc
    13321 tgtggcatgt tatcggcaat tttgcgcatg tagaaacgca gcagattatc cgccatgtct
    13381 tcagccagtt cgaagcgggc gacaatttcg cgatgaactt tgtcactgca acaacaaccg
    13441 atactgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag caagccgtgg
    13501 tggtattcaa aacccaacgt ccaggccagc gttgccagcg gttccagcag attaacaatc
    13561 ttattttcaa tacgtgcgtg ggcaattttg atatccatgc gcgtggaacc gatggtgcga
    13621 tgtacgcgca tatatttgcc atcgataaat tcacctttca gggtggccag actttcacgc
    13681 tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt acgttgaggg
    13741 tagatctcac gtagcttatc catcacttcg aagatatttt gctgcaatgg catctggtca
    13801 tgaccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac gtcaaaataa
    13861 ctgtcgaggc gtttacgtaa tccgttttcg tcggcaggta agtacttacc gatggcgtag
    13921 cccagcggca gcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca caaaaactcg
    13981 gttttatcag taccgtggcg ctccgaacat ccgcgccaga acatagtgcg ggtaatgcca
    14041 aatccattgt aaatatgcgg cagttgcccg gacatgccaa aggaatccgg taaataacct
    14101 attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag attacggacg
    14161 atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc gataatcaac
    14221 ttaccggctt ctacctgttt cttcacgcgg tctttgtttt ccggtttcac cgcgaaataa
    14281 tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt gtcctgttcc
    14341 agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc ttcggtggtg
    14401 aaataccact cccgatccca atgcatatgc ggggtgatgt gaacgcgaga taccgctttc
    14461 atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg ccatgcttaa
    14521 ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc accaatgccg
    14581 caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga agtaaaaaga
    14641 gcgagaaaat gccggcaccg ggtgtcgaaa ggccgatatt catcgcaccg acaatagcgc
    14701 ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct tcaatcgcca
    14761 tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat ttcccggtct
    14821 caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt acggtaaatg
    14881 ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg ttcgccattg
    14941 cgcccaggca gaatgcataa gcggctttgt tcactggccc tccaaggtca aaggaacaca
    15001 taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg ttcagccagg
    15061 cggtaagcga gttattgatc catgcgacag gttctcccac cacaaacagc atcagactgc
    15121 ccgctcccaa cgtaccgagc accgggtaga gataaaaagt caggaatcca ttgaatttac
    15181 tgctaagacg caagtgattt ttcacccagc gcatcaagta accggctatc aatccgccaa
    15241 cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc gcaaagcctg
    15301 gcgctaacgc cggtttatct gccagagaat aggcggtata ggccgcgagc accggtacca
    15361 tcaaaattcc gagcagcccg ccgcccagct tgcggtacat ccacagccag gagttttctt
    15421 cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc gccagcaccg
    15481 tgccccccgc gacaatcaac ggtacggcaa aagagattcc gctcaacagt gcctgtttca
    15541 gctccgtttt gacacttttc accggttgcg tatcttgctg tacggtacgc gtctcatcgc
    15601 tacgcttgag ggtaagcgct tgttggatca acgcttctgc atggcgaatc ggctcggcaa
    15661 caggcactga aagcgcggga atgccattaa aacgctcact ctccttgatg gcgacttcag
    15721 ccgcaaaaat acaggcggtt gcactattga gttgatccgc cgttaaacgc ccttcaatgc
    15781 cgttagcgcc ttgtttttca acgtaaacat ttacgccgag tttgcgtccg gctttttcca
    15841 gatattccgc agccatatag gtgtgagcaa tacccgccgg acaggccgtt acgcagacga
    15901 tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca tccagcgccg
    15961 agagcaactc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca tcatccgcaa
    16021 ggcgcgtggt cagcgctgtc agcagttgca tatgcgtagt acccgcttca ttggggggaa
    16081 tcgccagcag caccactaaa tcaactgctt ccgggccatc aacgccttcc cactgaagcg
    16141 gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt ttgccatgcg
    16201 gcacagccaa cccttcacct aaggcggtcg ggccaaggct ttcacggcga tacacttctt
    16261 ccagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga gtcaacgcgt
    16321 ggatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg cggtgggtca
    16381 gagtcgtcag attcatcatg atcctcactt gatcgttttt atagtcgcgc cagtgtgccc
    16441 gataaaacaa taccatcatg tgatcaattt cacttgattg aaattatttg tattgtattt
    16501 gtattattta caggatgaat ttatcgggca taatggctaa attcgtatta atgagatact
    16561 aaaaatggga cacaagccct tataccggca gattgccgat cgcattcgtg aacaaattgc
    16621 gcgtggcgag cttaaacctg gcgatgcgtt acccactgaa tcagcgctac aaacagagtt
    16681 tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ctagtcgagc agcagatcct
    16741 cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt
    16801 tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaaat
    16861 cctgatattt gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgc
    16921 gcaggatcgt gtgtggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct
    16981 ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa
    17041 ttcgaaatat cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga
    17101 aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa
    17161 gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag
    17221 ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc
    17281 gcgataagca caaaaaaggc catcatacga tggccaacca tgtcgaaacc gacagccctt
    17341 cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat ttatttcaga
    17401 acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac gcctgcggct
    17461 tccagagcag cgaatttctc atccgcagtc cctttcccac cagcaatgat cgcacccgcg
    17521 tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacctac aactggcttg
    17581 gtaacgtgct ctttgatgta cgctgccgct tcttcttcag cgctaccgcc gatctcaccg
    17641 atcatcacga tcgcttcggt ctgcggatct ttttcgaaca tttcgaggat gtcgataaag
    17701 ttagagcctg ggatcgggtc accgccgata ccaacacagg tcgactgacc gaaaccgtaa
    17761 tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac gatacccact
    17821 ttacccggtt tgtgaatgtg acccggctgg ataccgattt tgcattcacc cggagtgata
    17881 acgcccgggc agttcgggcc gatcatacga acgccggctt cgtccagttt cactttcaca
    17941 gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt gatgcctgca
    18001 tcaatagctt ccagaatgga gtctttgcag aacggtgctg gtacgtagat aacagaagcg
    18061 gtagcgccag tagcagcaac agcttcacgc acggtgttga acaccggcag gccgaggtgg
    18121 gtagtaccgc ctttacctgg ggttacgccg ccaaccattt tagtgccgta tgcaatggcc
    18181 tgttctgagt ggaaagtccc ctggctacca gtaaagccct ggcagataac cttggtgttt
    18241 ttatcaatta aaatggacat tatttcccct ccactgcggc aacaacctgc tgagctgcat
    18301 ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc ttcgcgccga
    18361 gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc acttctgcta
    18421 ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg aagatgttaa
    18481 ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg gttacacgtt
    18541 ctttggttgc gccgccgcca acgtcaagga agttagccgg ttcgccgccg tgcagtttaa
    18601 cgatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg atgttaccgt
    18661 ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga tcttcctgcg
    18721 actggtcacg catttcgcgc agatcaggct ggcggaacag tgcgttgccg tcagcgccca
    18781 gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg ttgatttcga
    18841 tcaacgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt ttggtgaact
    18901 gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc tgatacggca
    18961 tcgggccagt cagcggatca agcgcaactt tgtggatcag gtgcggagtt tcttccgcca
    19021 ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacacga cgggaactac
    19081 ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgctgct tcaaccagaa
    19141 tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc ttgccgagcc
    19201 agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca ccgcccgctt
    19261 taccgcggcc accagcgtga acctgacatt tcactaccca cgggccggca ccgatttttg
    19321 aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt gctggtaagc
    19381 catagcgggc aaaaagttgt tttgcctgat attcatgtaa gttcatgtgt tctgtccatc
    19441 cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc ggtctacagt
    19501 gcaggtgaag cttaaactac tacacgtcca gcagcagacg cgtcggatct tccagcaact
    19561 ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg
    19621 acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc atcggacgat
    19681 ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca
    19741 tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa
    19801 ccgtcagctt gccgtcacgg cctttaactg ccagctcttt gattttcttc tcgatgtctg
    19861 ccatgccgag ggtatcgaca tcacgcagta ccggcgtcac caggccgcgc ggcgtggaaa
    19921 ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcaccgtcg atagaagcat
    19981 tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc
    20041 ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa
    20101 tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca
    20161 gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg
    20221 cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct ttcgccgggg
    20281 ctttcgccag atgtttttcc acatcttcac gagtcagacg accacccaca ccggtgcctt
    20341 taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc gggcttaacg
    20401 catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct
    20461 cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga
    20521 tctgacgaga cgttaccgtt gtaccttcat cttccagaac cgcatccaga atgccgtctg
    20581 ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac
    20641 gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt
    20701 caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac
    20761 gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac
    20821 cgccggagag gcggaggccg tgcggcctgc ataacgcaga gaagccccaa acggaatcac
    20881 ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca
    20941 ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg
    21001 gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg
    21061 ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt
    21121 cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc
    21181 gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca ctaccagcgg
    21241 acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg ccggggtaga
    21301 cggaacgcaa acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc
    21361 ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca ccagaccaca
    21421 catccggccc catttctgtt cgccagaaga gatgaactgg tcgataacca cctgcgcacc
    21481 gttggcgaag tcaccgaact gcgcttccca gatggtcaga gtgcgtggtt ctgcggtggc
    21541 ataaccgtat tcaaacgcca gcactgcttc ttcagacagt acggagtccc agacgcggaa
    21601 cgcgccctgc ccgttatgga tgtgttgcaa cggcgtgtaa gtggaaccgt tagactggtt
    21661 gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg
    21721 aaccggaatg ccttcgtcaa ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc
    21781 gaacagtttc tcaccggcag ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga
    21841 ctgcatttca actgcttccg gcaccgtgct gatgcgtttc gccagctcct gcaggcgctt
    21901 catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt
    21961 gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc
    22021 atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc
    22081 cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct gatacatcag
    22141 cggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcacaccag
    22201 gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa
    22261 ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa ccatcttacc
    22321 gatatcagta cagtacggtg tagaacgagc atccagtgga ttagaggtgg tgaaaccaac
    22381 ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat
    22441 gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat
    22501 tggcagcact ttgttgctgc tcggctcatc aagtctgtcc agacgggcac gaacagaacc
    22561 gataactacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg ccaggtgcac
    22621 caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcacccgt
    22681 gccgaggtgt tctttatgtt taccggcaaa ctcgtcgaac aagtcttgcg gttttttacc
    22741 cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccaagaa ccacttcgcg
    22801 ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta acgcgtcacc
    22861 gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac gttcaaggcc
    22921 ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga aagtcgcgcg
    22981 accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtaa tgtgcatata
    23041 ctcggcacca atcgggccac agtaggtttg cttgagggct tccagcagct cgccgagttt
    23101 catgttttct ttgccgctgg caaatgaacc gacgttgaag gtctcctgga agtctgcttc
    23161 ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag
    23221 cggatcgaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac
    23281 tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc
    23341 tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc
    23401 cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt
    23461 taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga
    23521 gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg
    23581 cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag
    23641 ctcttgtcta tgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca
    23701 gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggccta
    23761 cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga
    23821 tgcgacgctt gcgcgtctta tcaggcctac ggtttacgca ttacgttgca acaacatcga
    23881 cttaatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt
    23941 catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc
    24001 agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc
    24061 gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag
    24121 aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg
    24181 agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata
    24241 gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat
    24301 cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt
    24361 cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacggcagg agcggcggaa
    24421 cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg
    24481 accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt
    24541 atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat
    24601 cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga
    24661 ttccgactct ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa
    24721 gtcgaagcgg ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc
    24781 atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc
    24841 gctggaagtg tcatccagac gggcattttt cagacgctcg cggatcactt tcaactgctc
    24901 aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc
    24961 ttgcagcgct ttacgaatcg ccaccggatc ttcaccgtta cgattattgt tccagcggtt
    25021 cagacgatcc agagatgctt caacatcaga ctcgctggca tcgcgcaatg cgccctgttc
    25081 ggcgatagac tcttgcagat gcagacctgc cgcacgacca aagaccacca ggtccagcag
    25141 cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt caccaacggc
    25201 aaacagcccc ggaacaacca catcttcgcc tttctcgttc accgtcagcg cctgaccggt
    25261 cactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg gaatcggctc
    25321 tttcaccgga tcgacgtgag caaaggtacg ggaaagctcc aggatacccg gcagacggga
    25381 ttcgagaact tctttaccca ggtgatcgag tttcagtttc gcgtgtggcc cccacggacc
    25441 atcacagccg cgaccttcac ggatttcaat catgatggaa cgcgccacca catcacggcc
    25501 cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag
    25561 cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatgcc
    25621 ggtcgggtgg aattgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat
    25681 gccgacaccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa tacgccctgc
    25741 cccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac cggtttcgat
    25801 gcacagtgcg gtacaaccca ccaccgcgcc atcctggttt ttcaccagat ccagcgcata
    25861 ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag
    25921 tgcgtgaccg gtacggtcag ccgccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt
    25981 tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcaagac gcgagaacgg
    26041 caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat
    26101 cgcgtcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat gccattccca
    26161 gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg
    26221 ggaacgggtc gggaagactt tagagagcag cgcacaggtc tggccgctct gggaaatttg
    26281 cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa attctctgac
    26341 tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt
    26401 gcaacgacaa tcaccagttg cagcatcagg cgcaatgcca gcggtttaac gtagtcggtc
    26461 aacacctgcc acatgccgat ccaggcatga atcaagatag aaaacagcgc cagcagggtg
    26521 aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca
    26581 ctggtggcga aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg
    26641 cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata
    26701 cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc
    26761 gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac
    26821 ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga
    26881 agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca
    26941 gaagccacag caggatgccc actgcaacaa aggtgatcac accggaaacg cgatggagaa
    27001 tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt
    27061 gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg
    27121 actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg
    27181 tctgcttgcg ggtcagacag cgtcctttct ataactgcgc gtcatgcaaa acactgcttc
    27241 cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag
    27301 acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat atagtttgtc
    27361 gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa tttttaatca
    27421 tctaatttga caatcattca acaaagttgt tacaaacatt gccaggaaaa gcatataatg
    27481 cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta
    27541 ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta
    27601 agtacttgat tcgccattta ttcgtcatca atggatcctt tacctgcaag cgcccagagc
    27661 tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag
    27721 ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag
    27781 gagaccttaa atggctgata caaaagcaaa actcaccctc aacggggaca cagctgttga
    27841 actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc
    27901 aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg aatctaaaat
    27961 tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct
    28021 ggcgaccgat tctaactacc tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac
    28081 tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca
    28141 gattacccgt ctgttccatg ctttccgtcg cgactcgcat ccaatggcag tcatgtgtgg
    28201 tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca
    28261 ccgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta
    28321 caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa
    28381 cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga
    28441 acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc
    28501 caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat
    28561 tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga
    28621 agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga
    28681 ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac
    28741 cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct
    28801 ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa
    28861 actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc
    28921 ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag
    28981 cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga
    29041 aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt
    29101 ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc caaacacaaa aaattgtaaa
    29161 aataaaatcc actatcggac attcatatat tctaaaaaaa atatatcggt ataaattata
    29221 aacaaatcga tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa
    29281 tatagcgcac ccctataat
//
LOCUS       DAZRVY010000043        29167 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZRVY010000043 DAZRVY010000000
VERSION     DAZRVY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.043"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..186)
                     /locus_tag="ACHXOX_001943"
     CDS             complement(<1..186)
                     /locus_tag="ACHXOX_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR7005414.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIE"
     gene            complement(364..1275)
                     /gene="gsiD"
                     /locus_tag="ACHXOX_001944"
     CDS             complement(364..1275)
                     /gene="gsiD"
                     /locus_tag="ACHXOX_001944"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR7005415.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1278..2198)
                     /gene="gsiC"
                     /locus_tag="ACHXOX_001945"
     CDS             complement(1278..2198)
                     /gene="gsiC"
                     /locus_tag="ACHXOX_001945"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR7005416.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2216..3754)
                     /gene="gsiB"
                     /locus_tag="ACHXOX_001946"
     CDS             complement(2216..3754)
                     /gene="gsiB"
                     /locus_tag="ACHXOX_001946"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR7005417.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3774..5645)
                     /gene="gsiA"
                     /locus_tag="ACHXOX_001947"
     CDS             complement(3774..5645)
                     /gene="gsiA"
                     /locus_tag="ACHXOX_001947"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR7005418.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5632..6597)
                     /gene="iaaA"
                     /locus_tag="ACHXOX_001948"
     CDS             complement(5632..6597)
                     /gene="iaaA"
                     /locus_tag="ACHXOX_001948"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR7005419.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6801..8036
                     /gene="moeA"
                     /locus_tag="ACHXOX_001949"
     CDS             6801..8036
                     /gene="moeA"
                     /locus_tag="ACHXOX_001949"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR7005420.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8036..8785
                     /gene="moeB"
                     /locus_tag="ACHXOX_001950"
     CDS             8036..8785
                     /gene="moeB"
                     /locus_tag="ACHXOX_001950"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR7005421.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8964..9626)
                     /gene="fsa"
                     /locus_tag="ACHXOX_001951"
     CDS             complement(8964..9626)
                     /gene="fsa"
                     /locus_tag="ACHXOX_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR7005422.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9757..10656
                     /locus_tag="ACHXOX_001952"
     CDS             9757..10656
                     /locus_tag="ACHXOX_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR7005423.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10662..13094
                     /locus_tag="ACHXOX_001953"
     CDS             10662..13094
                     /locus_tag="ACHXOX_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR7005424.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13240..14055
                     /gene="ybiV"
                     /locus_tag="ACHXOX_001954"
     CDS             13240..14055
                     /gene="ybiV"
                     /locus_tag="ACHXOX_001954"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR7005425.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14207..15472
                     /locus_tag="ACHXOX_001955"
     CDS             14207..15472
                     /locus_tag="ACHXOX_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR7005426.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15439..16599)
                     /locus_tag="ACHXOX_001956"
     CDS             complement(15439..16599)
                     /locus_tag="ACHXOX_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7005427.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16837..18138
                     /locus_tag="ACHXOX_001957"
     CDS             16837..18138
                     /locus_tag="ACHXOX_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005428.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18153..20516
                     /locus_tag="ACHXOX_001958"
     CDS             18153..20516
                     /locus_tag="ACHXOX_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR7005429.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20696..22291)
                     /locus_tag="ACHXOX_001959"
     CDS             complement(20696..22291)
                     /locus_tag="ACHXOX_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR7005430.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGVDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22510..23430
                     /gene="ldtB"
                     /locus_tag="ACHXOX_001960"
     CDS             22510..23430
                     /gene="ldtB"
                     /locus_tag="ACHXOX_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR7005431.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23489..24607)
                     /locus_tag="ACHXOX_001961"
     CDS             complement(23489..24607)
                     /locus_tag="ACHXOX_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR7005432.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24604..25071)
                     /gene="mntR"
                     /locus_tag="ACHXOX_001962"
     CDS             complement(24604..25071)
                     /gene="mntR"
                     /locus_tag="ACHXOX_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR7005433.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25257..25385
                     /gene="mntS"
                     /locus_tag="ACHXOX_001963"
     CDS             25257..25385
                     /gene="mntS"
                     /locus_tag="ACHXOX_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR7005434.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25657..27240
                     /gene="opgE"
                     /locus_tag="ACHXOX_001964"
     CDS             25657..27240
                     /gene="opgE"
                     /locus_tag="ACHXOX_001964"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR7005435.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27289..27804)
                     /gene="ompX"
                     /locus_tag="ACHXOX_001965"
     CDS             complement(27289..27804)
                     /gene="ompX"
                     /locus_tag="ACHXOX_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR7005436.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27857..27922)
                     /locus_tag="ACHXOX_001966"
     CDS             complement(27857..27922)
                     /locus_tag="ACHXOX_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005437.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28157..29044
                     /gene="rhtA"
                     /locus_tag="ACHXOX_001967"
     CDS             28157..29044
                     /gene="rhtA"
                     /locus_tag="ACHXOX_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR7005438.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7054 a         8003 c         7327 g         6783 t
ORIGIN      
        1 ttcaatatct ttaaagaaat agccaacgtt tttctgaata agttcaaagc ggctatcggc
       61 acgttttacc gcgcgttccg ataccagttg atagttaatg atgaaaaaaa gaccaataaa
      121 tgacagtgct gccaataaaa ataaaattat cagccttatc tttatctttt cgtataaact
      181 cagcatgtta atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt
      241 cccttgctgc ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa
      301 cacttgctta gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg
      361 taactatcct ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa
      421 cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac
      481 catatccgct cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc
      541 gaggccgaga aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa
      601 aaacaccacg atagacgaga cggtcccagg caagatatga cgcaataaaa tggtcatatc
      661 gctggcacca atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt
      721 gccgcgcacc agtcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt
      781 agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc
      841 aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc
      901 caacaagccc agcaacgtcc cgatcgccgc accgataaac acggcaaaca cgcccgccgc
      961 cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac
     1021 gccaaaccag tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc
     1081 ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa
     1141 taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg
     1201 tgtacgcacc tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca
     1261 gttaaatagt cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca
     1321 tccaccacta agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc
     1381 accgggtaat cgcgcatttc tacggagtca acgagtaagc gtccaagtcc gggccagttg
     1441 aaaactttct ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg
     1501 gtcactaccg ggatcatcgc gttacgtagc ccgtgtttga ggacaaccca ggtttcgctc
     1561 acccctttcg ccctcgcggt acgcatataa tcttcgctta aaacatcgac aaacgacgcg
     1621 cgggtaaagc gcgccatcac ggctgccact gccgcgccga gcgtcaggga gggtaaaatg
     1681 tagtgctgcc aactgtctgc tcccacggta ggcagccagc ccagttcaac ggagaatacc
     1741 tgaattaaaa gcatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc
     1801 atactcaatc gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata
     1861 ccaaatatca ccgcccagac catactggtt atggtcagcc acagcgttgg cataaagcgg
     1921 ctggcaatct catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc
     1981 acagcattgc tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc
     2041 tgacgcacca gttctataac ctgcgcatca gcttcgagcc cggcaatcaa tcgcgccgga
     2101 tcgccgggca gcatatgcac aaataaaaac accagcaccg agacgataaa cagcgtcgga
     2161 atcaacccca gtaagcgttt gataacgtaa ttaagcattc cactccctgc gttgcttatt
     2221 gcaaatccgc gtcttcaaag ctgaagccgg tgtctggcat gatccaaaaa ccggtcaggt
     2281 ttttactgtg tgccgacacc agtttttcta ccaccagcgg gatccacggc gactcttgcc
     2341 agatgatatc ctgcgccgcc ttatacaagc gggtcttttc cgccggatca ttagttttca
     2401 gtgcctgagc caggaagtcg tccacctgtt tattgctgta aaatgcggta ttaaacagcg
     2461 tcggtggcca gttttgcgag gcgaacagtg gcgatagcgc ccagtccgct tcgccggttg
     2521 aagccgacca gccagtgtag aacatccgca cgccgctctc tttttgccct ttaccttcaa
     2581 cttctgccgc ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg
     2641 ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc
     2701 acagcgtggt actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca
     2761 ctggatcgta aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac
     2821 cagtagctgg cgttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg
     2881 cgtaattcag cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac
     2941 tgatataacg ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca
     3001 gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca
     3061 ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca
     3121 atcctggctg ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg
     3181 tatccagttc atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt
     3241 ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg
     3301 agaacggctg tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa
     3361 tattcttata caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat
     3421 tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg gaatttaatg ccttcccgca
     3481 atttcacggt gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt
     3541 tcagtttcat ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct
     3601 gagataacgt gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca
     3661 ccaccacatc tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg
     3721 ccactaaccc actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta
     3781 cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc
     3841 aacgagacgg ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac
     3901 agcagtacac gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat
     3961 ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc gccgtggacc aatttcaaca
     4021 atttgcccga gatacatcac cgccacacga tgactaatcc gctctaccac ggccatatcg
     4081 tgggagataa acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata
     4141 atctgcccgc gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt
     4201 ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca
     4261 tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg
     4321 cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa
     4381 tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata
     4441 tcccggcgca atgcctgaag tttgccgggt gacaaggtat cgattctctg accgttaaag
     4501 ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta
     4561 ccgctgccag actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact
     4621 ttctcaacgg catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa
     4681 cgggtgacaa gattacgcac tcgtaaaaca ggttcgccat ccaccaccgt tttctgctcg
     4741 atgggggggg cctgtttcgc tggatgttca agcgatatca acgggaaacg tcggggataa
     4801 tccaaccctt tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga
     4861 tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac
     4921 atcaccagta cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata
     4981 acgcccatcg acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata
     5041 gtgacatcca gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc
     5101 gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga gttgatgcgg ataacgtgaa
     5161 agaatggttt gtgcctcagg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc
     5221 gcttcttcac gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca
     5281 gtaaataccg ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg
     5341 cgaacatggc gcatttgtgc agcgctctgc tcgctaagtt caatcacttc gcgactgcgc
     5401 cgctgcaaca gcattttatc gcactgtact aaaccgcccg cctgttccaa caggcgcatc
     5461 aatgccaacg cagtcactga cttaccggag ccggattcgc caacaattgc cagcgtctca
     5521 ccgcgttgca gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata
     5581 aaggcaatat tcagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt
     5641 ggcaacggtg tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc
     5701 ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat
     5761 agcgattaag ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca
     5821 ggcttccgcg agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag
     5881 cgcgcggatg aagacttcgc ccgtgccgat acaagaaacc gccacactgg cgttattggc
     5941 gtagcatccg gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat
     6001 tccgcctgtg gacgtggctg ccgccaaatt gccgtctaaa tccaacgcca cggcccccac
     6061 ggtgcccatt ttctgttttt catccagtgg cgcaccgcta tggtcgagga cggttgcccc
     6121 ttccttgcgc gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggcga
     6181 gacgcgctcc atgccacgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac
     6241 atgcgggctt tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac
     6301 gcccgccacc gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg
     6361 ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag
     6421 cagacgcacc gcttccgtca ccacatccag cgcactttcg cccgcttcca gcattttctg
     6481 cccggtttca acaatggcag acaacgcctc aatgtagcgt aattcctgtt gcagactcat
     6541 ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt
     6601 attcgtccat taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta
     6661 ctcattcaat ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct
     6721 acgcccgagg tggcatacag tctgtggcgt ttttctgaca taataggcaa attcgatttt
     6781 gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc
     6841 ttaatgagat gctttctcgc gtcaccccac tgaccgccca ggaaacgctg ccactggtac
     6901 agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc gcttgatgtt ccggggtttg
     6961 ataactccgc aatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc
     7021 tgcccgttgc cggtaaatcc tttgccggtc agccgtatca tggtgaatgg cctgcgggca
     7081 cctgcattcg tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc
     7141 aggagcagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc
     7201 aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa
     7261 ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg
     7321 tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc
     7381 agccgctggg cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag
     7441 aacagctggg ttgcgaggta atcaacttag ggattatccg cgacgatccc catgccctgc
     7501 gcgccgcatt tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt
     7561 cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag atcgccttct
     7621 ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt
     7681 tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc
     7741 ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac
     7801 gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc
     7861 aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat
     7921 ttagctcctt tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg
     7981 acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc
     8041 ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga
     8101 ttttgacggt caggaggcgc taaaagattc tcgtgtgctg atcgtcggcc tcggtggact
     8161 cggctgcgca gcttcgctgt atctggcaag cgccggtgtc ggcaacctga cgctgctcga
     8221 cttcgacacg gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt
     8281 cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc
     8341 gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca
     8401 cgatctggtg ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg
     8461 ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac
     8521 ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga
     8581 gaacgcatta acctgcgtag aagcaggcgt gatggcaccg ttgatcggcg taattggttc
     8641 gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccga ccagcgggaa
     8701 aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc
     8761 ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac gcgacaagcg
     8821 tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg
     8881 ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat
     8941 ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc
     9001 tgctcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca
     9061 tccagtggca gagtaattga ttcacatcct gccagtaagc agtccagcgc ctgacgcggg
     9121 gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt
     9181 aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc
     9241 gcaacatatt ccgcacctgc cagcgccgac agcaaccctt gtgctgcgcc atataccgcg
     9301 gttcccagcg ttggaatccc ttccgctttt aacatcttaa tagctgccag cccctcggcg
     9361 gtcaccggaa ctttcaccac gatatccgca ataatagaac gcaatttacg tgcgtcattc
     9421 accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc
     9481 gcttcatgaa gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt
     9541 gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg
     9601 tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt
     9661 agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa
     9721 agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac
     9781 tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc
     9841 tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc aggatctgct gtatgacgca
     9901 cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc
     9961 gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta
    10021 accgactgct gtccgacaca ggcattaacc gtgtgtggtg aagtgaaaag cgttgaggag
    10081 atcatgacga ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggtttaacg
    10141 ctttcgggtg gtgagccctt tatgcagccg gaaatggcga tggcgctact gcaagccagc
    10201 cacgaggcag gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc
    10261 gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg
    10321 ccgtttaaac agtggaccga cggtaacgcc gccagagtgc tggataacct gaaaaaactc
    10381 gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac
    10441 gaaacctctg taaaagctat taccgatttt gccgccgacg agctgcacgt tggcgaaatt
    10501 catttcctgc cctaccacac gctaggcatc aacaaatatc acttacttaa tctgccctat
    10561 gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg actttgccca gcagtatgcc
    10621 tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg
    10681 acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaaaccgc
    10741 cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc
    10801 cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga
    10861 tcaaacacga tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct
    10921 tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg
    10981 gtgctggctt tgcggtgagc gaagagaaca aacgcgttct gcatgaagtg tgcccgtggt
    11041 ggcgcggtca gaccgtacag gatcgctgct acggcatgtt taccgatgag caaaaaggtc
    11101 tgctggcgac cggaatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg
    11161 cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg tctgcgcgag aaagtagcgg
    11221 aacgtcgctc gcgcatcaat ctgacggtac tggaagattt gcacggcgaa cagttcctga
    11281 aagcgattga tatcgtgctg gtggcagtca gcgaacacat tgaacgtttt gcagccctgg
    11341 cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatag
    11401 cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac
    11461 tgtgttactt catccaattg atattgcaga tcgaatctaa cggtcactcg gtatcgtttg
    11521 gtcgtatgga ccagtatctc tacccgtact atcgccgcga cgttgaactc aaccagacac
    11581 tggatcgcga acacgccatc gaattgctgc atagctgctg gctgaaactg ctggaagtga
    11641 acaagatccg ctccggctca cactcaaaag cctctgcggg aagtcctctg tatcagaacg
    11701 tcaccattgg cggacaaact ctggttgatg gtcaaccaat ggacgcggtg aatccactct
    11761 cttacgctat cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc
    11821 gttaccatgc aggaatgagt aatgatttcc tcgacgcctg cgtacaggtg atccgttgcg
    11881 gcttcgggat gccggcgttc aacaacgacg aaatcgtgat cccggaattt attaaactcg
    11941 gtattgaacc gcaggacgct tacgactacg cagcgattgg ttgtatcgaa accgccgtcg
    12001 gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc
    12061 tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa
    12121 aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt aatggacgcg tgggatacgc
    12181 aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg
    12241 aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga
    12301 aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gttggcattg
    12361 ccaacctcgg caacagcctg gcggcagtga agaaactggt atttgaacag ggcgcgattg
    12421 gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac
    12481 tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata
    12541 cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccgc
    12601 gctacggtcg tggtccggtt ggcggcaact attacgcggg tacctcgtct atctccgcta
    12661 acgtaccgtt tggcgcgcaa actatggcaa caccggacgg gcgtaaagcc cacaccccgc
    12721 tggcagaagg cgcaagcccg gcctccggta ctgaccatct tggccctact gcggtcattg
    12781 gctcagtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac
    12841 tgaatccggc aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta
    12901 ccttctttga agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc
    12961 tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggct
    13021 attccgcctt cttcaccgcg ctctctccag acgcacagga cgatatcatc gcccgtactg
    13081 aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc cgggctaaat cattcacatc
    13141 atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt
    13201 acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc
    13261 acagacatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg
    13321 gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag
    13381 tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa
    13441 aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat
    13501 gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc
    13561 ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca
    13621 aaacactacc atcgcctgaa agctgtaaaa gattatcagg agattgacga cgtactgttc
    13681 aagttttcgc tcaacctgcc agatgaacaa atcccgttag tgatcgacaa actgcacata
    13741 gcgctcaatg gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt
    13801 cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg
    13861 caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt
    13921 tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc
    13981 gatgataata atcatgaagg cgcgctgaat gtgattcagg cggtgctgga taacacatcc
    14041 ccttttaaca gctgacctct ctaccggagc cgtgcgctcc ggttttccct tctttttaca
    14101 tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact tatattgtcg
    14161 catgcaaatc agaatggttt cgaatgaaag ttaatgagag gtggttatgg cttctacttt
    14221 taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct
    14281 gcgggcgcag cttggcgacg tccagcagat ctttaatcag ctaagcgatg acattgccac
    14341 gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc
    14401 ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg
    14461 cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc
    14521 gatgctggac tatctggacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa
    14581 tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc
    14641 gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct
    14701 gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc
    14761 tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa ggtcttggag cgcataccga
    14821 ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cgcgttttcg ccaacgtctt
    14881 taatggcaat ctggcgcaat atgatccctg gcatgcggca catcgtacgg aagttgaaga
    14941 gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccaag gctggacagc
    15001 gctctctaat atgctgcctg gtcaggggct gctgcacgtt gtgcccattc ctgaagccat
    15061 ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt
    15121 agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt
    15181 aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca
    15241 ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc
    15301 accaatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg
    15361 cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac
    15421 gcttgccgac ctcaacattc acggtaagcg agcgttggga atcgacagct gagtgtcgta
    15481 cagcccttcc cgttccacgg cggtgttccc ttttcctggg cgaacccgcc gcactgactc
    15541 ccgcaccgcc aatcgtccgt gcagcagcag cgagcccacc ggagcatccg ggcgaatcag
    15601 ccgctgttgc aacatttgca ccgcttcatt gcctagctca tcgcgtggca catgcacagc
    15661 cgtcagggga acatcctcga ttgccgcaag gttaaagccg tcgatactca tcaccgagac
    15721 atcctgtggt acgcgcagac cacgacgatg tagcgcattt accgttcccg ccgccataaa
    15781 atccccgccg accagaaacg ccgtcggcaa cgtcttgcca ttggcggcat ccagccacgc
    15841 tccgaccttt tcttccgctt ctttagcgct aaaactgctg atattcagca aatgacggtc
    15901 atggtgaaag gccagattat gctgacgcca tgattcttta atccctgcaa accgctgctc
    15961 catagtgtag cggcgcaggc acatcacatt catcacctcc cggtgaccca tttcaaacag
    16021 atagcgggcg gcgaacgcgc caattaaccg atgatccggc gcaatcgccg ggaggcgcat
    16081 tcgttcatca cggcagttga tcagcacaca aggcttagcg agatcggcgg ccagatcatg
    16141 gatgtgcgga tcgtcaatcc ccagcaggat cgctgcctgc gtatctgctt cattcatccg
    16201 cgagagaaag agcgcgggat cgctgtcaaa ctcctccagc gcgcagtaac gcagccgcac
    16261 ttcatgcggc gataacgctt tgctgatgct ctggatcaca cggtaataaa agatatcaga
    16321 tcgttggtca aaagcgcgtt gcggcgcgaa aataatcagg ttgttcagca acatacgacc
    16381 tgctgccatg ccatccatca cgcccaactc acgcgcgcag gccagcacct gttcgcgagc
    16441 gcgtgcactg gtattcgctt tgccagccag cacccgcgaa accgtactcg gcgatagccc
    16501 ggtacgtgcg gcgatatcgg cgattttcag cttcttgtcc attttgtgat ctgcctctaa
    16561 ttcggaaatg acagaatttt catgagcaaa aaagcccatt ctgtctggca ttaaatccat
    16621 ttcagtaatg tatatcagtc actcatggaa aaacgtgcac aaaacccgct ttctttcgct
    16681 tttagggtct cttactgtga attggtagaa caacttccat acaaaaataa tggaaaaacg
    16741 aacaataaag actttattta tcaaaaagtt aaaagtggtg taacaactcc attctgacgt
    16801 tgccgcacaa accctatacc attctgtgga gtgcatatga gtcagggtat caataacgat
    16861 atgactgcca gtaaaccgcg ccatatcgtc aaaaatctgc gttggtggat actggtcctt
    16921 tttttgctcg gtgttactgt caactacatc acgcgtaact cgctgggtat tctcgccccc
    16981 gaactgaaaa gcagcctagg gattaccacc gaacaatatt cctggattgt aggtgctttc
    17041 cagctcgcct ataccctatt ccaaccgttg tgcggatggc tgattgacgt cattggcctc
    17101 aaactgggct ttatgatctg cgcgggactg tgggcactgg cctgtctgat gcatgcgggg
    17161 gcgggaaact gggtacatct ggcgctgttg cgcttcttta tgggcggtgc ggaagccgcg
    17221 gcaaccccag caaacgccaa aaccatcggt gaatggtttc caaaatcaga acgcccgatc
    17281 gccgcaggct gggcaggcgt cggtttttcc atcggtgcca tgctggcccc accgattatc
    17341 tattttgccc acgcttcatt cggctggcag ggtgcattta tgttcactgg catcctggcg
    17401 atgctgtggg tggtgctgtg gtgggcgttc tatcacagcc cggataagca tcctaacctc
    17461 agcaaagccg agctggactt tattcgccag gataacgaag cgcccccggt caagttaccc
    17521 tttctgaccg cactgaaaac cgtgggcaaa aataaacgct tttacggtat cgctatcccg
    17581 gcctttatgg cagaaccggc gtgggcggtg atgagtttct gggtgcctct atacctgtcc
    17641 aaaacctatg gcatggacct gaagcagatc gcattattcg cctggttgcc gttcctcgcc
    17701 gctgatcttg gcagtattgc cagcggctat ctgaccaaac tttacacccg cctgtttggg
    17761 ctgagtcgtg tgaactccgt tgtcgcatca tcggtcagtg gcgcattctt gatggtttcg
    17821 ctggcggtga tggcctttac caataacccg tatatcgcca tcgtccttat ctccatcggc
    17881 ggcttcggtc accagatcat ctcctgcatg ctgagcgcac tggtggtgga atccttcgac
    17941 aaaggtcaga tggccactgt taacggcatg cgcggttctg ccgcgtggat cgcaagtttc
    18001 ctgttctccc tgattatcgg ggttaccgct gacaaaatcg gctttaaccc cctgtttatc
    18061 gccatgggct tctttgacct gattggcgct ttcttcctgg tcacctttat tgctgaacgt
    18121 cgcgcaaaac gcgcctgaga gtggatgtaa acatgaaaac gctgaaaaac tggaccctgc
    18181 acaaacagtc tgcccaccac gttgaactgc tggtcgatgg ccagcatatc ctgtgcctgt
    18241 acgtgctgga agaaaatctg ttccgcgtgc tgttaaaacg caaaggcgag ctggcgctag
    18301 atcgcacctg gagcattgct ccggcgcagg atgtgccatg ggaagggcgc agccgcgaag
    18361 atctcagcgg ctttagcctg cccgcgtgga cagtgatcca gcacgacgac accgtggtta
    18421 tcgccaccga gtcactgcgt gcaaccgttc accagccgct gtggctggag tggcactatc
    18481 gtaacaacga aggcgaatgg ctgccgttgg tgaatgaccg cccgaccagc gcttatctgc
    18541 tgaatgccca cggcgatggc gtggcacact atcagagccg tcgtaaagat gaacgctttt
    18601 atggcctcgg agataaagcc ggcgatctgc aacgcaacgg taaacgttac gagatgcgta
    18661 acctcgacgc gatgggctac aacgccgtta gtactgaccc gctgtacaaa cacattccat
    18721 tcaccatcac ccgccgcgat gacattagct tcggcctgtt ttatgacaac ctcagcagct
    18781 gctggctgga tctgggcaac gagatcgaca actaccacac cgcctatcgc cgctggcagg
    18841 cggaagcggg tgatatcgac tactacctgt ttaccggcga acgcgtactt gatatcacca
    18901 aagccttcgt acgcctgacc ggaaaaaccc tgttcggacc gaaatggagc ctgggctaca
    18961 gcggttcaac tatgcactac accgatgcgc cggacgcaca aaatcagttg atgaacttta
    19021 tccgcctgtg tgaagaacat gcaattccct gcgattcgtt ccagctctct tccggttata
    19081 cctcaattaa cggcaaacgc tatgtgttca actggaacta cgacaaagtg ccgcagccga
    19141 aagtgatgag ccaggcattt cacaatgctg gtttgcacct ggccgccaat atcaagccgt
    19201 gcctgttgca ggatcaccca cgctataacg aagtggcaga aagaggactg ttcatccgtg
    19261 attcagaaac agatgaacca gaacgttcca gcttctggga tgacgaaggt tctaacctcg
    19321 actttactaa tccgcagacc atccagtggt ggcaggaagg cgtgaccaca cagcttctgg
    19381 agatgggtat cgactccacc tggaacgaca acaacgaatt cgaagtatgg gacggtgaag
    19441 cacgctgtca cggctttggt cagacgatcg cgatcaaaca tattcgccca gtgatgccac
    19501 tgctgatgat gcgagcctcg atggaagccc agcagcgttt cgctccggaa aaacgtccgt
    19561 acttgatctc ccgctccggc tgcgcgggaa tgcagcgcta cgtgcagacc tggagtggcg
    19621 ataaccgcac tagctgggac actctgcgct acaacacccg catgggtctg gggatgagcc
    19681 tctccgggct gtataacctc ggtcacgacg tcggcggttt ctccggtgat aagccggacc
    19741 cggaactgtt tgtacgctgg gtacaaaacg gcgtgatgca tccgcgcttt acgattcact
    19801 catggaacga tgaccatacc gtaaacgaac cgtggatgta tccgggcgtc actccggcga
    19861 tccgcagcgc tattgagttg cgctatcgcc tgctgcccta cttttatacc ctgatgtggc
    19921 aggcatacgc cgatgatgaa ccaatgctac gtccgacttt ccttgaccac gagcacgacg
    19981 ttcagacttt tgaagagtgt gatgacttcc tgcttggtcg cgatattttg gttgccagcg
    20041 tcgtagagca aggcgagcgc cagcgccgtg tatggctgcc agataacgaa actggctggt
    20101 acgatttcta caacggcgaa tggttcagtg gcggtcagtg gatcaccctc gacgcaccgc
    20161 tggagaaact gccgctgatg gttcgtgccg gggctggtct accgctcagc aaacgcatca
    20221 cctatgtgag cgcagaacaa gacgataccc gcgaactgaa actgttcccg ctgaaaggtg
    20281 tgggcacaac gtccggcctg ctgtttgaag acgatggcga aagctggggc taccaaactg
    20341 gtaatgcgct ttgggtggaa tgggaaatgg tttgcgatgg ggcgaccatc aacctgaaag
    20401 tcaacgcgcg cggggattat cgtcctgcat ggagtgcact gaaagtgtca ttaccattgg
    20461 aagaaaaacg cacgctgctg gtcaatggtg ttgaagggag tgaatggatg cgttaaatca
    20521 ttaccggggc cgggaggcga ctgcacctta cccggtccag ccatcgaatc ctttacctca
    20581 gtatttcacg ctgcaggaag gcagcaaacc tgtaaacccc gggagcttaa ataagtaagt
    20641 gaccggggtg aacaggtgaa gccaatgcat ctgccgcatg aaagacggcg gggaattaag
    20701 cgtcgatccc tttactacgc aggtaatctt cgtaattacc gctaaagtcg atcgcgcgtt
    20761 ccggggtgat ttccagaatg cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa
    20821 caaagatcag cgtgccctga tacagttcca gcgccatgtt cagtgactcg atggattcca
    20881 tatccaggtg gttggtcggt tcgtccatga tcagaatgtt cggcttctgc atcattaact
    20941 taccaaacag catccgccct ttttccccac cggaaagcac tttagctggc tttttgatgt
    21001 cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt
    21061 cctgcttcca ctgactcatc cattcgaaca cggtcagatc attttcaaac tcatattcgt
    21121 ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg
    21181 gttgcagatc gcccaccagc gttttcagca gcgttgattt accgacgccg ttggtaccca
    21241 gtaccgcaag tttttcaccg acctccagca gcaggttgag atttttaaac agtggaccgt
    21301 tatcaacccc tttggtcaga ccttccactt ccagcgcgtt acggaacagt ttcttatcct
    21361 gttcaaaacg gatgaacggg ttctgacggc tggaggcttt cacctcttcc agtttgattt
    21421 tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc
    21481 ggctaacgaa agattgcaac tcagcaatct gcgctttctt cttggcgtta tcggccagca
    21541 gacgttcacg cgcctgggtc gccgccgtca tgtactcatc gtagttaccc ggataaacgc
    21601 gcagctcgcc gtaatccaga tccgccatgt gggtacagac catgttaagg aagtgacggt
    21661 cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag cacctgttcc agccagcgaa
    21721 tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag gagaatatcc ggatcagcaa
    21781 acagcgcctg cgccagaagc acacgcagtt tccagccagg agcaacttca ctcatcgggc
    21841 cgtagtgctg ttccactgga attcccacgc caagcaacag ttcaccggcg cgagcttccg
    21901 cagagtaacc gtccatttcg ccgtatttaa cttccagatc ggccacttta tagccgtctt
    21961 cctcactcat ttccggcaaa gcatagatgc ggtcacgctc ctgcttcact tcccacaact
    22021 ctttatgccc catgatcacc gtatccagca cagtgaactc ttcaaaggca aactgatcct
    22081 gacgcagctt accaatgcgc tcgttgggat cgagggaaac gttacccaaa gtcggctcta
    22141 agtcgccgcc gaggatcttc ataaaggtgg atttaccact accgttcgcg ccaatcaggc
    22201 cgtaacggtt gccgccgccg aatttgacgg aaatgttttc aaacaacggc ttactgccga
    22261 actgcatggt gacgttactg gaaactaaca caggcgtatc ctgaaaagag atatgacaaa
    22321 ccgcgcatta tgccataagt ctgaaagaag atcctgctcc tcgcttaagg tctacactca
    22381 agccaaaggc aaaaaatgtg atttcgtaca catctgattt cactgtgagc tggaatgaac
    22441 ttataatgcg cttccaatac tctaatattc tcaacccaat ggcctgccag gcacaaaatc
    22501 tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag cggccttcgc tgttgtcggc
    22561 ttttgcagta ccgcctctgc ggtaacttat cctctgccaa ccgacgggag tcgcctggtt
    22621 ggtcagaatc aggtgatcac cattcctgaa ggtaacactc agccgctgga gtattttgcc
    22681 gcggagtacc agatggggct ttccaatatg atggaagcga acccgggtgt ggataccttc
    22741 ctgccgaaag gcggtactgt actgaacatt ccgcagcagc tgatcctgcc ggataccgtt
    22801 catgaaggca tcgtcattaa cagtgcagag atgcgtctgt attactatcc gaaagggacc
    22861 aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag gcaaagatac gcctatcaac
    22921 tggaccacca aagttgagcg taagaaagca ggcccgacct ggacgccgac cgccaaaatg
    22981 cacgcagagt accgcgctgc gggcgaaccg cttccggctg tcgttccggc aggtccggat
    23041 aacccgatgg ggctgtatgc actctacatc ggtcgcctgt acgctatcca tggcaccaac
    23101 gccaacttcg gtatcggcct gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac
    23161 atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg tacagtttat tgatgagccg
    23221 gtaaaagcga ccaccgagcc agacggcagc cgttatattg aagtccataa cccgctgtct
    23281 accaccgaag cccagtttga aggtcaggaa attgtgccaa ttaccctgac gaagagcgtg
    23341 caaacagtga ccggtcagcc agatgttgac caggttgttc ttgatgaagc gattaaaaac
    23401 cgctccggga tgccggttcg tctgaattaa tcttcaaaac ttaaagcaaa aggcggactc
    23461 ataatccgcc ttttttattt gccagaccct agttggccgg gagtataact aacaaaacat
    23521 atcccaccaa cgccgcccac aacagcatcg gtattgaata gagatggaaa cgccaccaga
    23581 tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc cagcgatccg ggtaataacc
    23641 caaagccacc tacgtttacc gcccatacca gtaataaaga cggcggcaca tagttcagca
    23701 acaatatggt actcggcacg ttactgatca cctgcgataa accgattgcc gttaaccata
    23761 acccgggttc agatagatga ctcacgttac ccaacacgcc ttgcaacgct ggaagctggg
    23821 tcagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac
    23881 tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc cacaatcacc agtccccaca
    23941 gctcttgttt gaactccagc gccgtcagaa agacgatata cagccccaga caactccaca
    24001 ccagccgcgg tttccactcc ggtgtttgca cccccgtatg gtattgcagc gcctttccag
    24061 ggaaacaaca ccagcacagg agcaggagcg tcagcatcat tgcgccagcc agcggtgcca
    24121 tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg
    24181 ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca
    24241 gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga acaacaataa
    24301 acagcgcgac atcgttggtc agaaaggtag aaagcagcgc cgccgccagc accataaaca
    24361 tcgccagccg acgctccgta gcaaagcggc gcaccatttt gcgccccagc acatcaaaat
    24421 aaccgcttaa ctccacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt
    24481 gccagtcgat agcagcaggc caggatttcg gtgcaaaggg cacgaaaaag cttaatccga
    24541 taccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca
    24601 ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa gcgtcgagcg tctcttcact
    24661 aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac
    24721 caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc
    24781 ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag
    24841 ccgcttaagc attttagcca ccgtcggttg cgaaactccc agacgagcag ccatgtccac
    24901 ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat
    24961 aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt
    25021 cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc
    25081 tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg
    25141 cgaaggtaca aaaaattaac gttttagcaa tagctacata atatagcctg tgctatatct
    25201 gtatgtaatg caatcatccc tcaaggatcg acgggattag caagtcagga ggtcttatga
    25261 atgagttcaa gaagtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc
    25321 tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaagataaa ccttccgata
    25381 aatagcggcg tcgcggtacg ccgcttcact cctgctttta tgcaggcata acgcgttttg
    25441 gtctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc
    25501 agtagttgac ctgaacggcg gctcgctcta tcttcttgca gccctgcgta tattgcggct
    25561 cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc
    25621 agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg
    25681 cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta
    25741 ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct
    25801 attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtgtcagt
    25861 tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc taatttcaat
    25921 acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt
    25981 ccgtggtaca gctacctggt cggcttattt atttttgcgc tcggcgtaat agcaatcagg
    26041 cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg acagcctgtg tctggtattc
    26101 agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggtgg cgtatttaaa
    26161 ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac
    26221 gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg
    26281 gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg tgatcggcga aagcgcgcgt
    26341 cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt
    26401 aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa atcacttggc
    26461 ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc aggataactt tgtcaccctg
    26521 gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac
    26581 gatactgcta tcgccagcat cgccaaacgt gcagatgaag tgtacttcct gaaagaaggt
    26641 aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg
    26701 ctggcgcaag agcactcgca accgcagctg attgttctgc atctgatggg ctcgcatccg
    26761 caggcctgcg acaggacaca aggcaaatac gaaacctttg tgcaatcgaa agaaacgtcg
    26821 tgctatctct ataccatgac gcaaacggac gatttactgc gcaagctgta cgatcagtta
    26881 cgcaacagcg gcagcagctt ctctctggtt tacttttctg accacggtct ggcctttaaa
    26941 gagcgcggca aagacgtgca ataccttgcc catgatgata aatatcagca aaatttccag
    27001 gtgcctttta tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc
    27061 tcagccaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata
    27121 aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc
    27181 cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa
    27241 aacaaaaaat ccgccccgag aggcggattt tttatatcac caaagtgatt agaagcggta
    27301 accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct gctcgtaaga
    27361 gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt aggagaaacc
    27421 gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt taccataacc
    27481 cacccccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg gaccagcagt
    27541 gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc ttttctcggt
    27601 gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc ggtatttcag
    27661 gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt aaccgccagt
    27721 tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg cggccagtgc
    27781 tgaaagacat gcaatttttt tcataaccac ctcaaatgtg attcaaataa gtcctaagtt
    27841 ttaaatatat caaaaattaa tgggaaactc ttcgcgattt gtgatgtcta acgggccatt
    27901 tcatgtaaca gaacgtttcc atacaccgct atccatctaa atttaaatca ctttttcaga
    27961 gaactgcgta agtattacgc atgttttccc tgtcattcat ccagattatt cctaatcacc
    28021 agactaatga ttccatcaat cctggcgcat tttagtcaaa acgggggaaa attttttcaa
    28081 caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt aagtctagat
    28141 atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt taccaatagt
    28201 catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta agtcactttt
    28261 tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct
    28321 catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt
    28381 gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta ttcagacagt
    28441 accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg cgctgttctc
    28501 ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct
    28561 gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc tggcactggg
    28621 ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag cggaacatgg
    28681 ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta attttcgtgc caattggagc
    28741 gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc tggctgtcgc
    28801 tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc gtttgccaac
    28861 acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt ccgggatgat
    28921 tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta tcatcgccgc
    28981 ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat tagacattaa
    29041 ttaaatttac atttctgcat ggttatgcat aaccatgcag aatttctcgc tacttttcct
    29101 ctacaccgtc tttatatatc gaattatgca aaagcatatt tattccgaaa attcctggcg
    29161 agcagat
//
LOCUS       DAZRVY010000044        28894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZRVY010000044 DAZRVY010000000
VERSION     DAZRVY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.044"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHXOX_001968"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHXOX_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR7005439.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHXOX_001969"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHXOX_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHXOX_001970"
     CDS             complement(2649..3497)
                     /locus_tag="ACHXOX_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR7005440.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHXOX_001971"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHXOX_001971"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR7005441.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHXOX_001972"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHXOX_001972"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR7005442.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHXOX_001973"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHXOX_001973"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR7005443.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHXOX_001974"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHXOX_001974"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR7005444.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHXOX_001975"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHXOX_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR7005445.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHXOX_001976"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHXOX_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR7005446.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHXOX_001977"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHXOX_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR7005447.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHXOX_001978"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHXOX_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR7005448.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHXOX_001979"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHXOX_001979"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR7005449.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHXOX_001980"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHXOX_001980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR7005450.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHXOX_001981"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHXOX_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHXOX_001982"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHXOX_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHXOX_001983"
     CDS             22885..23868
                     /locus_tag="ACHXOX_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR7005451.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHXOX_001984"
     CDS             23891..25402
                     /locus_tag="ACHXOX_001984"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR7005452.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHXOX_001985"
     CDS             25427..26425
                     /locus_tag="ACHXOX_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7005453.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHXOX_001986"
     CDS             26458..27552
                     /locus_tag="ACHXOX_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR7005454.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHXOX_001987"
     CDS             27564..28436
                     /locus_tag="ACHXOX_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR7005455.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="ACHXOX_001988"
     CDS             complement(28484..>28894)
                     /locus_tag="ACHXOX_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR7005456.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7144 c         7865 g         7019 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgccagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAZRVY010000045        28050 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZRVY010000045 DAZRVY010000000
VERSION     DAZRVY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.045"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOX_001989"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOX_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR7005457.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHXOX_001990"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHXOX_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHXOX_001991"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHXOX_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHXOX_001992"
     CDS             complement(1702..3318)
                     /locus_tag="ACHXOX_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR7005458.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHXOX_001993"
     CDS             3614..4330
                     /locus_tag="ACHXOX_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR7005459.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHXOX_001994"
     CDS             complement(4327..5988)
                     /locus_tag="ACHXOX_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR7005460.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHXOX_001995"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHXOX_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR7005461.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHXOX_001996"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHXOX_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR7005462.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHXOX_001997"
     CDS             7443..7775
                     /locus_tag="ACHXOX_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR7005463.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHXOX_001998"
     CDS             complement(7777..8991)
                     /locus_tag="ACHXOX_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR7005464.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHXOX_001999"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHXOX_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR7005465.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHXOX_002000"
     CDS             complement(10153..11490)
                     /locus_tag="ACHXOX_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005466.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHXOX_002001"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHXOX_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHXOX_002002"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHXOX_002002"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR7005467.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHXOX_002003"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHXOX_002003"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR7005468.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHXOX_002004"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHXOX_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR7005469.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHXOX_002005"
     CDS             15153..15809
                     /locus_tag="ACHXOX_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005470.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHXOX_002006"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHXOX_002006"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR7005471.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHXOX_002007"
     CDS             complement(16782..17180)
                     /locus_tag="ACHXOX_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR7005472.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHXOX_002008"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHXOX_002008"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR7005473.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHXOX_002009"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHXOX_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR7005474.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHXOX_002010"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHXOX_002010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR7005475.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHXOX_002011"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHXOX_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR7005476.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHXOX_002012"
     CDS             23741..23821
                     /locus_tag="ACHXOX_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005477.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHXOX_002013"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHXOX_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR7005478.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHXOX_002014"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHXOX_002014"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR7005479.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHXOX_002015"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHXOX_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR7005480.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHXOX_002016"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHXOX_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR7005481.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHXOX_002017"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHXOX_002017"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR7005482.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7571 c         7159 g         6560 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcactcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZRVY010000046        27470 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZRVY010000046 DAZRVY010000000
VERSION     DAZRVY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.046"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHXOX_002018"
     CDS             complement(<1..81)
                     /locus_tag="ACHXOX_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR7005483.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHXOX_002019"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHXOX_002019"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR7005484.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHXOX_002020"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHXOX_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR7005485.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHXOX_002021"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHXOX_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR7005486.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHXOX_002022"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHXOX_002022"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR7005487.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHXOX_002023"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHXOX_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR7005488.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHXOX_002024"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHXOX_002024"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR7005489.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHXOX_002025"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHXOX_002025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR7005490.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHXOX_002026"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHXOX_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHXOX_002027"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHXOX_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR7005491.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHXOX_002028"
     CDS             complement(12387..12626)
                     /locus_tag="ACHXOX_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR7005492.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHXOX_002029"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHXOX_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR7005493.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHXOX_002030"
     CDS             13003..13290
                     /locus_tag="ACHXOX_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR7005494.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHXOX_002031"
     CDS             13960..14103
                     /locus_tag="ACHXOX_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR7005495.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHXOX_002032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHXOX_002032"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR7005496.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHXOX_002033"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHXOX_002033"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR7005497.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHXOX_002034"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHXOX_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR7005498.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="ACHXOX_002035"
     CDS             complement(16292..16750)
                     /locus_tag="ACHXOX_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR7005499.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHXOX_002036"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHXOX_002036"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR7005500.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHXOX_002037"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHXOX_002037"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR7005501.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLNNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHXOX_002038"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHXOX_002038"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR7005502.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHXOX_002039"
     CDS             19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHXOX_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR7005503.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHXOX_002040"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHXOX_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR7005504.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="ACHXOX_002041"
     CDS             complement(21526..23124)
                     /locus_tag="ACHXOX_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR7005505.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHXOX_002042"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHXOX_002042"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR7005506.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="ACHXOX_002043"
     CDS             complement(24803..25381)
                     /locus_tag="ACHXOX_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR7005507.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHXOX_002044"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHXOX_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR7005508.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="ACHXOX_002045"
     CDS             26820..26999
                     /locus_tag="ACHXOX_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR7005509.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27418)
                     /locus_tag="ACHXOX_002046"
     CDS             complement(27119..27418)
                     /locus_tag="ACHXOX_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR7005510.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
BASE COUNT         7067 a         6771 c         6941 g         6691 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttattgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aattgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagc
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
//
LOCUS       DAZRVY010000047        27102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZRVY010000047 DAZRVY010000000
VERSION     DAZRVY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.047"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHXOX_002047"
     CDS             <1..921
                     /locus_tag="ACHXOX_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005511.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXOX_002048"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXOX_002048"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR7005512.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXOX_002049"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXOX_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR7005513.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHXOX_002050"
     CDS             3882..4277
                     /locus_tag="ACHXOX_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR7005514.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXOX_002051"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXOX_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR7005515.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXOX_002052"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXOX_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR7005516.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHXOX_002053"
     CDS             complement(5586..6626)
                     /locus_tag="ACHXOX_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7005517.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHXOX_002054"
     CDS             complement(6699..7334)
                     /locus_tag="ACHXOX_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR7005518.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXOX_002055"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXOX_002055"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR7005519.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHXOX_002056"
     CDS             complement(7960..8403)
                     /locus_tag="ACHXOX_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR7005520.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXOX_002057"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXOX_002057"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR7005521.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHXOX_002058"
     CDS             complement(8743..9246)
                     /locus_tag="ACHXOX_002058"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7005522.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHXOX_002059"
     CDS             complement(9240..9764)
                     /locus_tag="ACHXOX_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR7005523.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHXOX_002060"
     CDS             9973..10968
                     /locus_tag="ACHXOX_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR7005524.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHXOX_002061"
     CDS             10977..11855
                     /locus_tag="ACHXOX_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR7005525.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHXOX_002062"
     CDS             12061..13068
                     /locus_tag="ACHXOX_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR7005526.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXOX_002063"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXOX_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR7005527.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXOX_002064"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXOX_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR7005528.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXOX_002065"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXOX_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR7005529.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXOX_002066"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXOX_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR7005530.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXOX_002067"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXOX_002067"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR7005531.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXOX_002068"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXOX_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR7005532.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXOX_002069"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXOX_002069"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR7005533.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXOX_002070"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXOX_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR7005534.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXOX_002071"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXOX_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR7005535.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXOX_002072"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXOX_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR7005536.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXOX_002073"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXOX_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR7005537.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHXOX_002074"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHXOX_002074"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6421 a         7516 c         6809 g         6356 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcagtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgataa gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcaa tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca agctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ct
//
LOCUS       DAZRVY010000048        25774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZRVY010000048 DAZRVY010000000
VERSION     DAZRVY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.048"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="ACHXOX_002075"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="ACHXOX_002075"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR7005538.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="ACHXOX_002076"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="ACHXOX_002076"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR7005539.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="ACHXOX_002077"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="ACHXOX_002077"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR7005540.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="ACHXOX_002078"
     CDS             2440..3063
                     /locus_tag="ACHXOX_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR7005541.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="ACHXOX_002079"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="ACHXOX_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR7005542.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /gene="yadW"
                     /locus_tag="ACHXOX_002080"
     CDS             complement(3447..3512)
                     /gene="yadW"
                     /locus_tag="ACHXOX_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR7005543.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="ACHXOX_002081"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="ACHXOX_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR7005544.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            complement(4969..5016)
                     /gene="yadX"
                     /locus_tag="ACHXOX_002082"
     CDS             complement(4969..5016)
                     /gene="yadX"
                     /locus_tag="ACHXOX_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR7005545.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="ACHXOX_002083"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="ACHXOX_002083"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR7005546.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="ACHXOX_002084"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="ACHXOX_002084"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR7005547.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="ACHXOX_002085"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="ACHXOX_002085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR7005548.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="ACHXOX_002086"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="ACHXOX_002086"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR7005549.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="ACHXOX_002087"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="ACHXOX_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR7005550.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="ACHXOX_002088"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="ACHXOX_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR7005551.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="ACHXOX_002089"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="ACHXOX_002089"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR7005552.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="ACHXOX_002090"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="ACHXOX_002090"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR7005553.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="ACHXOX_002091"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="ACHXOX_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR7005554.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="ACHXOX_002092"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="ACHXOX_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR7005555.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19928..20824
                     /gene="gluQRS"
                     /locus_tag="ACHXOX_002093"
     CDS             19928..20824
                     /gene="gluQRS"
                     /locus_tag="ACHXOX_002093"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR7005556.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            20884..22281
                     /gene="pcnB"
                     /locus_tag="ACHXOX_002094"
     CDS             20884..22281
                     /gene="pcnB"
                     /locus_tag="ACHXOX_002094"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR7005557.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="ACHXOX_002095"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="ACHXOX_002095"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR7005558.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="ACHXOX_002096"
     CDS             23127..23711
                     /locus_tag="ACHXOX_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7005559.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="ACHXOX_002097"
     CDS             23816..24556
                     /locus_tag="ACHXOX_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR7005560.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25774
                     /gene="htrE"
                     /locus_tag="ACHXOX_002098"
                     /pseudo
     CDS             24591..25774
                     /gene="htrE"
                     /locus_tag="ACHXOX_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6986 c         6416 g         6042 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc tgtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc gccacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgcg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctca agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgcgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAZRVY010000049        25309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZRVY010000049 DAZRVY010000000
VERSION     DAZRVY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.049"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            279..1253
                     /gene="hemB"
                     /locus_tag="ACHXOX_002099"
     CDS             279..1253
                     /gene="hemB"
                     /locus_tag="ACHXOX_002099"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR7005561.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1359..2210)
                     /gene="tauD"
                     /locus_tag="ACHXOX_002100"
     CDS             complement(1359..2210)
                     /gene="tauD"
                     /locus_tag="ACHXOX_002100"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR7005562.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2207..3034)
                     /gene="tauC"
                     /locus_tag="ACHXOX_002101"
     CDS             complement(2207..3034)
                     /gene="tauC"
                     /locus_tag="ACHXOX_002101"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR7005563.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3031..3798)
                     /gene="tauB"
                     /locus_tag="ACHXOX_002102"
     CDS             complement(3031..3798)
                     /gene="tauB"
                     /locus_tag="ACHXOX_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR7005564.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3811..4773)
                     /gene="tauA"
                     /locus_tag="ACHXOX_002103"
     CDS             complement(3811..4773)
                     /gene="tauA"
                     /locus_tag="ACHXOX_002103"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7005565.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5383..6054
                     /locus_tag="ACHXOX_002104"
     CDS             5383..6054
                     /locus_tag="ACHXOX_002104"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR7005566.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            6064..7260
                     /locus_tag="ACHXOX_002105"
     CDS             6064..7260
                     /locus_tag="ACHXOX_002105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR7005567.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            7260..7820
                     /locus_tag="ACHXOX_002106"
     CDS             7260..7820
                     /locus_tag="ACHXOX_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR7005568.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            7822..8595
                     /locus_tag="ACHXOX_002107"
     CDS             7822..8595
                     /locus_tag="ACHXOX_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR7005569.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            8783..9058
                     /gene="frmR"
                     /locus_tag="ACHXOX_002108"
     CDS             8783..9058
                     /gene="frmR"
                     /locus_tag="ACHXOX_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR7005570.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            9093..10202
                     /gene="frmA"
                     /locus_tag="ACHXOX_002109"
     CDS             9093..10202
                     /gene="frmA"
                     /locus_tag="ACHXOX_002109"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR7005571.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10295..11128
                     /gene="fghA"
                     /locus_tag="ACHXOX_002110"
     CDS             10295..11128
                     /gene="fghA"
                     /locus_tag="ACHXOX_002110"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR7005572.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11354..11893)
                     /locus_tag="ACHXOX_002111"
     CDS             complement(11354..11893)
                     /locus_tag="ACHXOX_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR7005573.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(11995..13205)
                     /gene="mhpT"
                     /locus_tag="ACHXOX_002112"
                     /pseudo
     CDS             complement(11995..13205)
                     /gene="mhpT"
                     /locus_tag="ACHXOX_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
     gene            complement(13492..14505)
                     /gene="mhpE"
                     /locus_tag="ACHXOX_002113"
     CDS             complement(13492..14505)
                     /gene="mhpE"
                     /locus_tag="ACHXOX_002113"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR7005574.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(14502..15452)
                     /gene="mhpF"
                     /locus_tag="ACHXOX_002114"
     CDS             complement(14502..15452)
                     /gene="mhpF"
                     /locus_tag="ACHXOX_002114"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR7005575.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(15449..16258)
                     /gene="mhpD"
                     /locus_tag="ACHXOX_002115"
     CDS             complement(15449..16258)
                     /gene="mhpD"
                     /locus_tag="ACHXOX_002115"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR7005576.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(16268..17134)
                     /gene="mhpC"
                     /locus_tag="ACHXOX_002116"
     CDS             complement(16268..17134)
                     /gene="mhpC"
                     /locus_tag="ACHXOX_002116"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR7005577.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(17152..18096)
                     /gene="mhpB"
                     /locus_tag="ACHXOX_002117"
     CDS             complement(17152..18096)
                     /gene="mhpB"
                     /locus_tag="ACHXOX_002117"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR7005578.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(18098..19762)
                     /locus_tag="ACHXOX_002118"
     CDS             complement(18098..19762)
                     /locus_tag="ACHXOX_002118"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR7005579.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            19804..20637
                     /locus_tag="ACHXOX_002119"
                     /pseudo
     CDS             19804..20637
                     /locus_tag="ACHXOX_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            20714..21796
                     /gene="lacI"
                     /locus_tag="ACHXOX_002120"
     CDS             20714..21796
                     /gene="lacI"
                     /locus_tag="ACHXOX_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR7005580.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            21919..24993
                     /gene="lacZ"
                     /locus_tag="ACHXOX_002121"
     CDS             21919..24993
                     /gene="lacZ"
                     /locus_tag="ACHXOX_002121"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR7005581.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNHLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            25045..>25309
                     /locus_tag="ACHXOX_002122"
     CDS             25045..>25309
                     /locus_tag="ACHXOX_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005582.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         6007 a         6977 c         6481 g         5844 t
ORIGIN      
        1 aaacacaaac cagataaata tcttatcaat atacaacaaa gtttgaatta ttcaatttat
       61 agcagcacaa ccaaacgcca aaaaaataat aaccaagagc tataaataaa gattaaaaat
      121 attttcagtg aatatgaaat tattgctatt tctattacaa agatgtcctg gtctctcttt
      181 cattctcttc ttgtcaacat cgcgacaact ttcgtaaaac actcctgccc cgcttcaggt
      241 atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc
      301 ctcgtcgcct acgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc
      361 ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg
      421 aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca
      481 tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa
      541 ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc
      601 agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg
      661 gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag
      721 gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga
      781 tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg
      841 cgattatgtc gtattcgacc aagttcgcct cttcctttta tggcccgttc cgtgaagctg
      901 ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg
      961 aggcgattcg tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac
     1021 ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg
     1081 gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta
     1141 tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc
     1201 tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt
     1261 cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc
     1321 ctctccccaa gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt
     1381 ttatccccaa ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg
     1441 gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa
     1501 cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc
     1561 tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt
     1621 ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc
     1681 gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat
     1741 ttacggaaat catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc
     1801 gcctcatagg ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct
     1861 ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag
     1921 ttgtcgttat ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct
     1981 tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt
     2041 tgctgctgcg gcgtaatagc ttgatcgcgc agaaatacca cctgatagcg cagcaccgca
     2101 tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt
     2161 tgtgcgccga tatacggccc cagcggggta atactcagac gttcactcat tgtacttctc
     2221 catgccaggg cgtcagacgg cgctgtaacg cgcgcagacc cagttctaaa agaaaggcga
     2281 taatcgcaat caccgcgatc cccgccagta ccacgtcagt cgcgagaaat tcacccgctg
     2341 actgaaccat aaatcctaaa ccgcgtgtcg cggcaatcag ctccgccgct accagcgtag
     2401 accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca
     2461 aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc
     2521 gaacctgctg cgcgcttttc acccccgcca gcaccgacat cgccaccggt gcaaaaatcg
     2581 ctaaatagat caataagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg
     2641 gcaaataagc cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc
     2701 gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga
     2761 gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca
     2821 taaatccctg cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca
     2881 aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg
     2941 ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc
     3001 gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac
     3061 gcggcttaaa acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct
     3121 cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc
     3181 agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg
     3241 ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat
     3301 ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg
     3361 attcgccgcc agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca
     3421 gatatagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc
     3481 cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac
     3541 attgcgccac ggtagtagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc
     3601 ctcaatacgc ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat
     3661 cagattcagc agggtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc
     3721 gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat cggcatagag
     3781 atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg
     3841 gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc
     3901 gatgattgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata
     3961 ggtattcccc ttcaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag
     4021 tttgctgatg ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg
     4081 agcatcgatg gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc
     4141 tttgcgcacc acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc
     4201 ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc
     4261 atcaatatca ccccgctgcc atgcggcgat aatcgcgggc ggctgcaggt tcacaatctc
     4321 cacttgccct ggtttaatcc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt
     4381 ggagataaac ggtacggcaa tgcgtttgcc aatcaaatct tcgggtttgc tgatagtttt
     4441 ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg
     4501 cacctgttgg ctggctgcaa ccgctaatgg gctggaaccg aggttgccga tttgcacgtc
     4561 gccagaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacgct
     4621 cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga
     4681 ggtttgatac gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc
     4741 ggcaagaagt gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg
     4801 ggagcagtat tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga
     4861 ttttattctt agcaaaaaaa cggcaaagga aagattaaat atgcaattac tccgttctaa
     4921 caattgggaa aattactaca cttcatttta gtgatgcata aacaaccaca ttacgagatt
     4981 tattacttca ttgtttctat caatacatat aataaaacta ttatattcgc attgtatata
     5041 tcactgtatc attctcaatc actcgcatat tacattcaca taatgtatat aaacaatact
     5101 tgttcacaga acatattttt taaatgcaat taattgacaa actaaatcac atctaaatac
     5161 aatgtataaa acaaaataac tatacatacc aaacatacaa acacttaaca tatgaattgc
     5221 ataaaaacat tacccagtcg aacctccggg tctgaaagcc cttgcttttc agttattagc
     5281 aacatttaac atttataaat taacatctgg ctgcgccaga gattacgtct ctgagtgcat
     5341 cgttgtgctt gtagtataat aaataaaatg gagtgtcatt aagtggataa ggttttagat
     5401 tcagccctcc tttcttcggc aaataaaaga aagggtatcc ttgctattgg cgcacatcct
     5461 gacgatatag aattaggctg tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat
     5521 atcgcagcag tggtgatgac taccggcaac tctggtacag atggaataat cgatcgccat
     5581 gaagaatcgc gcaacgccct aaagatatta gggtgccacc aaactattca tcttaatttt
     5641 gctgacaccc gcgctcattt acagctcaat gatatgattt ccgccctgga agacatcatt
     5701 aaaaatcaaa ttccttctga tgttgaaatc atgcgggtat ataccatgca tgatgccgac
     5761 cgccatcagg atcatctcgc tgtttatcaa gcttcaatgg ttgcctgccg cactattcca
     5821 caaattctcg gctacgaaac cccaagtacc tggctttcat ttatgcctca ggtttttgaa
     5881 tccgttaaag aagaatattt cacagtcaaa cttgctgcat taaaaaaaca taaaagccag
     5941 gaacgacgtg attatatgcg tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa
     6001 caggtcaata gcgatctggg tgaaggcttt gtaattcata aaatgattct ttgagcaaat
     6061 tgcatgaaaa cctggatatt tatctgtatg tccatagcaa tgttgctatg gtttttaagt
     6121 acgctaagac gtaaacccag tcaaaagaaa ggctgtattg acgccattat acctgcgtat
     6181 aacgaaggcc cgtgtctggc gcagtcactg gataatctac tgcgtaaccc ttatttttgc
     6241 cgggtaattt gcgttaacga cggctccacg gacaataccg aagcggtcat ggcggaagtc
     6301 aaacgcaaat ggggcgaccg ctttgttgcc gtcacgcaaa aaaataccgg taaaggtggt
     6361 gcgctgatga atggcctcaa ttatgccacc tgcgaccagg tttttttaag tgatgccgac
     6421 acctatgttc cgcccgatca agacggaatg ggctatatgc tggcagaaat taagcgcggt
     6481 gctgatgccg taggcggcat tccctctact gcgttgaaag gcgcggggct gttaccgcac
     6541 atccgcgcga ccgtaaagtt gccgatgatt gttatgaagc gcacgctaca gcagctcctg
     6601 ggcggcgcac cgtttattat cagcggtgcc tgcggaatgt tccgtactga tgtattgcgt
     6661 aagttcggtt tctcggatcg tactaaagtc gaagaccttg atctcacctg gacattagtg
     6721 gcaaacggct accgtattcg gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac
     6781 agtccgcgtg aggagtggcg tcgctggcgg cgttggattg tgggctacgc ggtctgtatg
     6841 cgcctgcata aaaggctttt atttagccgc ttcggtatct tcagtatatt tcctatgctg
     6901 ttggttgtga tttatggcgt tgggatttat ctcactacct ggtttaatga attcatcacc
     6961 accgggccgc atggtgtggt gttggcaatg tttccgctta tctggatcgg cgtagtttgt
     7021 gttattggtg cttttagcgc ctggtttcat cgttgctggt tgttggtgcc tttagcgccg
     7081 ctttccgttg tgtatgtatt attagcttat gccatctgga ttatctatgg acttattgcc
     7141 ttttttactg gacgcgaacc tcagcgcgac aaacccaccc gctattccgc actggtggaa
     7201 gcgtcaaccg cttattccca accttctgtc acaggaactg aaaaactatc tgaagcttaa
     7261 tgttaaagag aaaaattatt cagattgcag accaggtaat tattgatgaa actgcaggtg
     7321 aagttgttat cggcgcgaat acccgtattt gtcatggtgc cgttattcag ggtccggtag
     7381 tgattggcgc aaactgcctg ataggtaatt atgcgtttat tcgtcctggc acaataatca
     7441 gcaatggtgt aaaaattggt tttgccaccg aaattaaaaa tgcggttatt gaagcggaag
     7501 caacgattgg tccgcaatgt tatattgccg actcggtagt tgcaaaccag gcatatttgg
     7561 gcgcacaagt acgtaccagt aatcatcgtc tggatgaaca gcctgtgtct gttcgaactc
     7621 cagagggaat tatcgctacc ggatgcgata aattaggttg ttatatcggg cagcgttcac
     7681 gccttggcgt acaagttatt attttgcctg ggcgaattat ttccccgaac acgcaacttg
     7741 gcccgcgcgt gattgtagaa cgtaatttac ctaccggaac ttattcactc cgacaagaac
     7801 ttatccgtac aggagattaa aatgataaaa cggacgttat tagcggcggc catttttagc
     7861 gcattgcccg cttatgccgg gttaacttcc attaccgcgg gctacgattt taccgattat
     7921 tctggcgatc atggcaaccg taatttagcg tatgctgaac tggtggcgaa agttgaaaac
     7981 gcaacgctgc tttttaatct ttcccagggg cgtcgtgatt atgaaactga acatttcaat
     8041 gccactcgcg gtcagggtgc ggtctggtat aaatggaata actggctgac aacccgaacg
     8101 ggtattgcct ttgcggataa tacgccggtc tttgcccgcc aggattttcg tcaggatatt
     8161 aacctggccc tgttgccaaa aacgcttttc acgaccggtt atcgctacac taaatattac
     8221 gatgatgtcg aagtcgatgc ctggcaaggc ggcgtatcac tctatactgg cccggtgatc
     8281 accagctacc gctataccca ttatgactcc agcgatgcag gtggtagtta tagcaatatg
     8341 atttccgtgc gtctgaatga cccgcgcggc actggttata cgcaactatg gctaagccgc
     8401 ggaacaggcg cttacaccta tgactggacg ccagaaacac gctacggcag catgaagagc
     8461 gtcagtttgc aacgtattca accgctgact gagcaactta atctcgggct gacggcaggt
     8521 aaagtgtggt acgacacccc aaccgatgat tataacggtc tgcaacttgc agcccatctg
     8581 acctggaaat tctgattgct tctgccgccc gctatccggg gcggccttcc ctgccgatta
     8641 gccccactcc ctttcctcgt tgttttccga ccacattcac cggataaatt ttattctcca
     8701 gcgttatata ctatagggag gtatgcattg acatatagca tacctcccta tagtatattg
     8761 cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa aggtccttac
     8821 tcgagttcgt cgtattcggg ggcagattga tgctctggaa cggtcgctgg agggtgatgc
     8881 cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta acgggctgat
     8941 ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact gctacagccg
     9001 cgaagtcagc caatccgttg acgacactat tgaactggtt cgagcctatc ttaaatagct
     9061 gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc gttgcatttg
     9121 ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa aaaggtgaag
     9181 tgctgattaa agtcacccat accggcgttt gccataccga cgcatttacc ctctccgggg
     9241 atgacccgga aggtgtattc ccggtggttc tcggtcacga aggggccggc gttgtggttg
     9301 aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatcccg ctttacaccg
     9361 cggagtgcgg cgagtgtgag ttctgtcgtt ctggcaaaac taacctctgt gttgcggttc
     9421 gcgaaaccca gggtaaaggc ttgatgccag acggcaccac ccgtttttct tacaacgggc
     9481 agccgcttta tcactacatg gggtgctcta cattcagtga atacaccgtg gtcgcggaag
     9541 tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgcctg ctgggctgtg
     9601 gcgtgaccac cggtattggc gcggtgcaca acacagctaa agtccagcca ggtgattctg
     9661 ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg cgtcaggcga
     9721 aagcgggacg gattatcgct atcgatacca acccgaagaa attcgatctg gcgcgtcgct
     9781 tcggtgctac cgactgcatt aacccgaatg actacgacaa accgattaaa gatgtcctgc
     9841 tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac gtcaacgtga
     9901 tgcgtgcggc gctggaaagt gcgcaccgcg gctggggtca gtcggtgatc atcggggtgg
     9961 caggttctgg tcaggaaatc tccacccgtc cattccagtt ggtcactggt cgcgtatgga
    10021 aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg gttgaagatg
    10081 cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc cttgatgaaa
    10141 ttaatgacgc cttcgacctg atgcatgaag gcaaatccat tcgaaccgta attcgttact
    10201 gatttcccgc aggtataccc cgtccacttc agacggggtt cttaatactc tccccgggca
    10261 gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa aacatgccag
    10321 ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat gtgaaatgaa
    10381 tgtcggcgtc tatctcccac caaaagccgc gaatgaaaaa ttgccggtgc tgtactggct
    10441 ttcaggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc agcgttacgc
    10501 ggctgagcac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca gtcatgtcgc
    10561 agatgccgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg caacgcaagc
    10621 gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc cggatttagt
    10681 gatgcaacat tttccggcaa cgaccagaaa gtctatctct ggtcattcta tgggcgggct
    10741 gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct cggcgttttc
    10801 gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg catatcttgg
    10861 tgaaaataaa gatgcctggt tggattacga tccggtgagt cttatttcac aaggtcaacg
    10921 cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcgg aacagctgcg
    10981 gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa tccgttatca
    11041 cgagggttac gaccacagct attattttgt ctccagtttt attggcgagc atattgccta
    11101 ccacgccaat aaactgaata tgcgttgata atagtgcacg actgccggat gcggcgtgaa
    11161 cgccttatcc ggcctacact tcgcccgtaa accgtaggcc tgataagacg cgcaaagcgt
    11221 cgcatcaggc atggcgcacg actgccggat gcggcgtgaa cgccttatcc ggtctacact
    11281 tcgcccataa accgtaggcc tgataagacg cgcatagcgt cgcatcaggc atctgcgcac
    11341 gacttatcta tgtttaccac atcaaatcat caggcacttt gaagtcggca tacggatcgt
    11401 cttcatcctg ctcttcggca ctgagcgcgc tgtgcaacac aatactgctg gcatcacgct
    11461 gagcaatttt atcggcaaca ctggcgggaa taatggtgta ttcaccttcg ctattgttat
    11521 cgaccaacaa gcgggcaatc gccagacgac cattaatcaa ctgcgcctgt gtgagcttat
    11581 cgacaaaaat cttcttaatc agattgccgt cagtgaagtt aaaaccaata tcgccattgg
    11641 caatggtgat tcggttcatt tcaatgagct gcttcacctg ggctttatat tctttcgcca
    11701 acgccgcttg tttttgctgt tcgctaagct gtttatcacg ctcaagctgt gcctttttat
    11761 tttcttctac cgcagcccga gcttcacgcg cctgaacgcg cgattttttc gccgttctct
    11821 cgaccttcgc cgcttttttg ctggtgacta atcctgcttt gagcaactgc tcttgtaagg
    11881 taagttttgc catttttgtt tccaaacccg ctgaataatt tttagcgagt atacctgtaa
    11941 acgccgtggg tgtattgggg gcaagtccct ttcgcccctg cttaataagg cgcgtcaggc
    12001 atcggcacac ggctgcattc ttgatctccg actcatcaaa ataaacaccg ccaacccagc
    12061 aacaagaata cccggtgcag aagcggccat tacgccgacc gtgccagtgc ctaatgccag
    12121 cattttcccg gccagtaacg gaccgctcat agcccccaga cgccctaccg ccacggctgt
    12181 tcccacacct gttgcgcgga tctgcgaact gtaaaacaac ggtgccaggg catacaaaac
    12241 gctttgccca cctgtcgcaa acaaccccgc gacaaatccc gccagcaaca taccgttaaa
    12301 tgacgacacc gttccaagcg ccagcagcga agctaacatg ccgctataaa tcagtagcga
    12361 catggttact ggacgcagct tatccatcaa tgcgcccaac attaacgtcc cgcttgccgc
    12421 ccccatttgc agggcaaaca tcacccctgc cgcctgcgat ggctggaatc cttgctccac
    12481 caaaagtagc ggtagccagt tgatcaacat ataaaccacc agcagagtga agaaataaca
    12541 caaccacagc agcagcgtcg cggttgccgt ttctggcgca aataaggcac gcagtggtgg
    12601 cgcagcctgt ttttcgccag cgaaaaccgc tgactccggc agccagcgca ttaatagcgg
    12661 caccagaatc aacggcacca caccacctac ccaaaacacc gtttgccatg ctaagtttgc
    12721 ccccgcgaaa cccagtgtcg ccgccagcgc cgcgccaatg ggaacaccgc aatacatcag
    12781 gctcactgcc gtcccacgaa aacgtggacc cgcgcttcag atgtcagggc gataagattc
    12841 ggcaacgccg cccccagccc gacaccggtc atcagccgcg caaagaccag tgaggggaaa
    12901 tcccaggcaa tcgccgttgc cagtgagaac aaaccaaaca gcgcaactga gccaatcaaa
    12961 atacgcttac gaccataacg gtctgccagc atcccgccaa ccaacgcgcc gggtagcaaa
    13021 ccgagtattc cggcgctaaa tatccagccc atttgcattt tatcgagtgc gaaagcctgg
    13081 gcgatgccac ccgccgcaat gccagccgcc tgaagatcca gcccttccat cagagcgacc
    13141 aaaaaacaaa gcccgatggt cagcatcagg cgggatgaag atgatgaagg ggtacgagtc
    13201 gacataagat gaacctcatt gcgagtgcag gtgaatcgcg actgatacat tgccagccgc
    13261 gtaatcagaa ctatttaatg actgcctacg ggcgaaatgt agcgaaatgt aggccggata
    13321 aggcgttcac gccgcatccg gcagtcgtgc gcagatgcct gatgcgacgc taacgcgtct
    13381 tatcagacct acaaattctc acgccatccg taggccggac aaggcgttca cgccgcatcc
    13441 ggctgtcgtg ctgggatggg catacaaaat gcacgttatc ggcagtcatc attatttgtt
    13501 gttgcgcaga tccagcgcca cgtcgacgat catatcctcc tggccgccaa ccatccggcg
    13561 tttgcccagc tcaacgagaa tatccaccgc acttaagcca taacgcgccg ccgccgtttc
    13621 acagtgacgc aggaagctcg agtaaacacc agcgtatccc agcgccagcg tttcgcgatc
    13681 gactcgtacc ggacgatcct gcaacggacg caccaggtcg tcggcggcat ccattaacgc
    13741 atagagatcg gtcccatgct gccagcccag tttatccgcg gcggcaataa acacttccag
    13801 cggtgcgtta cctgcgcccg cgcccattcc cgcgaggctg gcgtcgattc ggtcgcagcc
    13861 ctcttccacc gccgcgatag agttcgccac gccaagactc aggttatggt gagcgtgcat
    13921 gccggtttgc gtttctggtt tcaacactgc tttcagggcg cggaaacggt cacggatatc
    13981 gctcatgttc atcgcaccgc cagaatccac cacataaata caggtcgcac cgtagccttc
    14041 catcagcttt gcctgcttgg cgagattctc cggcgtggtc atatggctca tcatcagaaa
    14101 accaacggtg tccattccga gctcgcgggc atactgaata tgctgggcgg aaacatcagc
    14161 ttcggtacag tgcgttgcca cacgaaccac ccgcgcgcca gcctgccagg catttttcag
    14221 atcgtgaata gtgccgattc ctggcagcaa caacgtcgcg attttggcgt gcctcaccac
    14281 atccgccgcc gcttcaatcc attcaaggtc gctatgtgcg ccgaaaccat agttaaagct
    14341 ggaaccttgc aaaccgtcgc cgtgggccac ttcaatcgaa tccacgcggg catcgtccag
    14401 tgctttggca atctggcgaa cgttttccag cgaatactga tgacgaatgg cgtgcatacc
    14461 gtcacgcaat gtgacgtccg agatataaag ttttttaccg ttcatgccgc ttctcctgcc
    14521 ttgcgcgcca gtgactgggc cattttttcc gctgtcgcca gcgcactgga agtcataata
    14581 tcgaggttgc ccgcataggc aggcagataa tgcgctgcgc cttcgacttc cagccagacc
    14641 gctgttttca gtccggagaa ttgccccacg cccggtaaat tgaccggttt atcctgcggg
    14701 ataacttcaa actgcacgcg ctgtttcagg cgataacccg gtacgtaagc ctgcaccgcc
    14761 tcagccattt cattgattga ggcttcgata tcatcttgtg aagcttcgtc gctcaataca
    14821 tacaccgtgt cacgcatcat caacggtggc tctgctgggt taagcacaat aatcgccttc
    14881 ccttttgccg cgccgcccac cacttcaatg gctcgggaag tggtttccgt aaattcatcg
    14941 atattggcac gcgtgccagg tccggcagat ttactggcga tagaagcgat aatttcggcg
    15001 taatgaacac gcgccacgcg tgaaactgcc gccaccattg gaatggtggc ctggccgccg
    15061 caggtgacca tgttgacgtt cagttgatcg acgttcgcct cgaggttaac caccggcacg
    15121 cagtaagggc cgatggcagc aggcgtcagg tcaattaagc gaatatccgg tttcgcttcg
    15181 cgtaaagcgg catcgttttt cacatgcgcg cccgcgctgg tcgcatcaaa tacaatgtcg
    15241 atatcagcaa attcaggcat gttcatcagc ccgatcaccc cttcatgggt ggtggcgacg
    15301 cccatacgtc tggcacgcgc cagaccgtcg gactgaggat caatgccgac catcaccgcc
    15361 atctccagat gctgaccgtg acgcaaaatt ttaatcatca gatcggtgcc aatgttgcca
    15421 gaaccgataa tggcgacttt acgcttactc atgacagact tccttttggg gctgcgcttg
    15481 aaaatgtcgc agcaactgaa cctatgcctt caatatgggc ttcaaaacga tcgcccgcat
    15541 tcaccgccac catcgggcct aatgccccgg taagaatgat atctccggcg cgcagcggtt
    15601 cacccagact ggccattttg cgtgccagcc agacggcggc attaagcgga tgtcccaggc
    15661 attcgctgcc gcgcccgctg gaaacctctt cgttattacg cgtcatcttc atggcgcagt
    15721 ttttcaggtc taaccccgcc ggacgttgcg ccggaccgcc gatgacatac accccgcagg
    15781 aagcgttatc tgccacggta tcgacaaact gaatcgacca gtcgcgaatg cggctcccca
    15841 ccacttccag cgccggaagt acccattcaa tggcgttata cagttcgtcg aaggtgatat
    15901 cagttgcggg taaatcgcgg ttcaacacca gtgcgatctc cgcttcaatg cggggttgca
    15961 gaacacggga aaaaggaatg atttcgttat cgccataaca catgtcggca aataacgtcc
    16021 caaaatccgg ttgatcaacg cccagttgtt gttgcacttt cggatgcgtc aggcccactt
    16081 tacgccctac cacgcgacgc ccctgcacaa cgtcatattg cacatttatg tgctgaatgg
    16141 cgtaggccgc ttcagcgtga tcgataccaa tcagatcgcg cagcggcgca atcgcttcgc
    16201 cctgctctgc ggcgcggcgt aaatccgccg ccagttgctc aagagtatgc ttcgtcatga
    16261 ccattcctta agggcgtgcg aggaaattca gcaccaattg attgaaagcg tcggcatgtt
    16321 cccactgcgc ccagtgacca cagtcgcgga agatatgcag ttcagaaccg gcaatgccgg
    16381 acagcagacg cagacccgca tccatcggca caaagcggtc gttgcgcccc cagacaatca
    16441 gggtttgcgc tttgatttcc gccagacgtg ggccaaaatc cgggaactgt ttcggattag
    16501 cttccaggct cttaacgaag ttttccaggt gatcgcggcg cgacagcata ttattcaggc
    16561 gcgcttcaaa cagggcgtcg gtcaaatcgc tggtatcgaa aacgaagata tccatcatca
    16621 gcttcaggtt ttcgatagtc ggctgacgat aaagctgatt cagtcgctta ataccttcgg
    16681 ttggcatcgg cgtaaacaaa ctcatgccgc ccgtaccgcc gcccatcagc accagtttgc
    16741 cgacgcgctc cggccagtta agggtgaacg ccacagaact atggccgccc atcgagttgc
    16801 ccagcaggtg gattttggcg atatccagtt gatccaccac gcttttcagg attcgtgcat
    16861 taagatccga tcgtgaacca ctattaacga tcgaatcgct tttgccccaa cccggacaat
    16921 ccagcaggat cacccgatag cccgcctcta ccagcggatc gatattgcgg ctgaagttcg
    16981 cccagccagt agcacccggg ccggaaccat gcagcaggac aacggtttcg tcgccttgtc
    17041 cgcagtcatt aaaatggatg cgcagcgttt tacccgcttc ttctacattc agaaaacggc
    17101 tggtggcggc ttcggtttgt ggctgataac tcatcatctt ctcctgcata ttcagttctc
    17161 tgttctggcg cttaacgagc caaatccggc aatccactcc gggattgggc ggtaataacg
    17221 cccttcgcta cgccagttgc caaatgcaga aatagcggca aaagcggcga cccaggtttt
    17281 gatttcatgt gtcgactttc cggcaatggc ggaaagctct tcgttactga cggcatccag
    17341 ttcctgtatg cgtccctgct ccagcaaagt catgaactgg ttatcccaaa tcgggttgag
    17401 cggatgcaga gttctctgat cctcaacaaa cttctcagcg gcgctaatca ccctttgctg
    17461 acgcaattcg cgctcactgg cgggtaaatc tttcccgctc cccaacagac ggtcgcgcat
    17521 atgggcatcg gctttcgcca gttcgggcac cggcggctga tgggaaagcc caccggaacc
    17581 caggaacagc acgcgtttat tgagagtgct ggtgaaacgt ccaatggctt cacccaacat
    17641 gcgggtacgc tggaaaccgg gcagcggcgt ggcgacaccg ttgatgaaca caggcagaac
    17701 tggcacctta tccagcccac cgagcaggaa ctccagcggc tgggcgaacc cgtggtccac
    17761 ctgcatacag taagaaacgg caagatcgat cccgctcttc atgacagcat gcgcacaggc
    17821 ctccgccagc tccacaggca cgggcagctc tcctgccgca ctgccgaaat caccaattgc
    17881 cgtcgctcca acgcctaaac agaacggtgg catcacgtca tagaaaaagc cgttgtagtg
    17941 atctggcgca aacagcacca ccagttcagg ggagaaagcc gcaatacgct cgcgggcgct
    18001 ggcaatcacg ccattgacct catcgagtac ctcttgcgcc gggtcgacat accccaccaa
    18061 cggcgagtgg gaaagacagt gaagataagc gtgcatatca ggctaccttt tcgacagaaa
    18121 cgtcggcatc agggcgggtc agcgtcatca ccgacgccag tttattcagc gtattgccca
    18181 gggtttgcgg aatggcggtg gcggcaacaa agcgatccgg gcgcatcacc accagcgaag
    18241 cattgtgttg cgcgaaccag ctacgcagac gaccttgcgt atcgcccacg tgtagcacgc
    18301 cgtcgtggtt atcctgtgcg gtatgaattt gcacttccgg caccacctga atgaagcgtg
    18361 tgcccaacgc gcgccactgc tggatttgct cgtcgctcat cccccacagt ggattgcatc
    18421 cccagccaat taccgcgaag ttcgcgccga tcgcgttatc gagcagcgtc acgtcgccgt
    18481 tttccagcgt gactttcggc tgaataaaca tcttgccgac cggagagtgc ttcgcttcgc
    18541 cctcacgcac cagcgcaccg ccgtaatatt gcggcatcgg cttgaagcgc atttcgagga
    18601 agtagcgttt tactggcggc agataattca acagccagga aacgccgtca cgtaacgtac
    18661 cctgccagcg tttcggcgga gccagcacgt tgcccgccgt cacggacagg tcaatcatcg
    18721 ctttggcgtg atcgcgacgt tcttgttgat aggtatcgag cagcgcatcg cgggctttcc
    18781 cctggataac caacgccagt ttccaggcga ggttaaaggc gtcgcgcata ccactgttat
    18841 agccctgccc ctgccacacc ggcatgatgt gcgcggcatc gcccgccagc agtacgcgat
    18901 caatacggaa acgttgcgcc agtcgcgcgt tatgggtgta gacacgctgg cgaatcaatt
    18961 caacattgtc cggattaggc agcactttgc ttaacagctt gcgcatattt tgcggctcac
    19021 gcagctgctc ttcggtttct cccggcatca ccataaattc aaagcgacgt accgcatgag
    19081 gcagcgcggc agaaacatac gggcgcaccg gatcgcaaca caaatagata tgcggcgtac
    19141 ttaacggatc gttggcgata tctaccacaa tccactgatt tggcgcagtt ttaccttcaa
    19201 acggcacatt cagggtgcga cgaacaaaac ttgccccacc atcacaggcc accagccact
    19261 gggctttgat cgtttcccgc tgcccttctg ccgttttcag gtgcaaggtc acttcgtcat
    19321 cttgctggct gaaggcctcc agctcgcggg aaaacaagca gcgcacattc ggaaaacgcg
    19381 acaacccttc cagcatcacc gcatcgacct gtggctgaat aaaggcgtta cggcgcggcc
    19441 agccaaattc atcggtcatt ggctgaatat cagcaaaaca gcggcctttt ggggtgagaa
    19501 aacgcatcgc gtgccacggt gtagtgtgcg gcagaacatt ctcgaccagg ccgaccgact
    19561 gcatggtgcg cagcgcctcg tcatcaatac caatcgcacg cgggtagtcg atcaacttat
    19621 cgagtttctc caccaccagc acgtcaatgc ccatctgacc gagatagttc gccatcatca
    19681 gcccaaccgg accggcacca gcgatcgcca cctgaacgct atggttaaca gcaggctgga
    19741 tgtcagggtg ttgtattgcc atttctgcac aacgttgttt taactataga aatgtcaatt
    19801 aatatgcaga acaatgagca gacggaatac aaaaccgtgc gcggcttaac ccgcggtcta
    19861 atgttattaa atatgttaaa taaacttgat ggcggtgcca gcgtcgggct gctggcggaa
    19921 ctcagcggcc tgcatcgcac cactgtgcgg cgactgctgg agacgctgca ggaagaggga
    19981 tatgtccgcc gtagcccctc cgatgacagt tttcgtctga ccatcaaagt gcggcaatta
    20041 agcgaaggat ttcgtgacga acagtggatt tctgcactgg cggccccgct gctgggcgat
    20101 ctgttgcgcg aagtggtatg accaacagat gtgtccaccc tggatgttga tgcaatggtg
    20161 gtacgcgaaa ccacccaccg tttcagccgc ttatcctttc accgggcaat ggtcgggcga
    20221 cgtttaccgc tcctgaaaac cgcctcgggc ctgacctggc tggccttttg cccggaacaa
    20281 gaacgcaagg aattaatcga aatgttagcc gcccgccccg gtgatgacta tcaactggca
    20341 cgagaaccgt taaagctgca agccattctg gcgcgcgcgc gcaaagaggg ttacggacag
    20401 aactaccgcg gctgggatca ggaggagaag atcgcctcta tcgccgtacc gctgcgcagt
    20461 gaacaacggg tgattggctg tctgaatctg gtgtatatgg cgagcgcaat gaccattgaa
    20521 caggcagcgg aaaagcatct tccggcgcta caacgggtag caaaacagat cgaagaaggg
    20581 gttgaatcgc aggctattct ggtggccgga cggcgaagcg gcgtgcattt acgttgacac
    20641 catcgaatgg cgcaaaacct ttcgtggtat ggcatgatag cgcccggaag agagtcaatt
    20701 cagggtggtg aatgtgaaac cagtaacgct atacgatgtc gcagagtatg ccggtgtctc
    20761 ttatcagacc gtttctcgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
    20821 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
    20881 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
    20941 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
    21001 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
    21061 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
    21121 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
    21181 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
    21241 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
    21301 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
    21361 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
    21421 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
    21481 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
    21541 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
    21601 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
    21661 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
    21721 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    21781 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    21841 aggctttaca ctttatgctt ccggctcgta tgttgtgtga aattgtgagc ggataacaat
    21901 ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
    21961 cgtgactggg aaaaccctgg cgttacccaa cttaatcacc ttgcagcaca tccccctttc
    22021 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    22081 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
    22141 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
    22201 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgcctttt
    22261 gttcccgcgg agaatccgac gggttgttac tcgctcacat ttaatattga tgaaagctgg
    22321 ctacaggaag gccagacgcg aattattttt gatggtgtta actcggcgtt tcatctgtgg
    22381 tgcaacgggc gctgggtcgg ttacggccag gacagccgtt tgccgtctga atttgacctg
    22441 agcgcatttt tacgcgccgg aaaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
    22501 ggtagttatc tggaagatca ggatatgtgg cgaatgagcg gcattttccg tgacgtctcg
    22561 ttgctgcaca aaccgagcac acaaatcagc gatttccatg ttgccactca ctttaatgat
    22621 gatttcagcc gcgcggtact ggaggcagaa gttcagatgt acggcgagct gcgcgatgag
    22681 ctgcgggtga cggtttcttt gtggcagggt gaaacgcagg tcgccagcgg caccgcgcct
    22741 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac gctacgtctg
    22801 aacgtcgaaa acccggcgct gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
    22861 gaactgcaca ccgacgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
    22921 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgcggc
    22981 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
    23041 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgttgttc gcattatccg
    23101 aaccatccgc tgtggtacac gctgtgcgac cgcttcggct tgtacgtggt ggatgaagcc
    23161 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggtta
    23221 cccgcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
    23281 atcatctggt cgctggggaa tgagtcaggc cacggcgcta atcacgacgc actctatcgc
    23341 tggatcaaat ctgtcgatcc atcccgcccg gtgcagtatg aaggcggcgg agccgacacc
    23401 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
    23461 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
    23521 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggcttcgc taaatactgg
    23581 caggcgtttc gtcagtaccc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
    23641 tcgctgatta aatatgatga aaatggcaat ccgtggtcgg cttacggcgg tgattttggc
    23701 gatacaccga acgatcgcca gttctgcatg aacggtctgg tctttgccga ccgcacgccg
    23761 catccagcgc tgacagaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
    23821 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gcttctgcac
    23881 tggtcggtgg cgttggatgg caagccgctg gcaagcggtg aaatgcctct ggatgttgct
    23941 ccacaagata aacagttgat tgaactgcct gaactaccgc agccggagag cgccggacaa
    24001 ctctggctta cggtacacgt agtgcaaccg aacgcgaccg catggtcaga agccggacac
    24061 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gcgtgacact ccccgccgcg
    24121 ccccacgcca tcccgcaact gaccaccagc gaaacggatt tttgcatcga gctgggtaat
    24181 aaacgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgctgaa
    24241 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
    24301 ggcgtaagtg aagcgacccg cattgacccg aacgcctggg tcgaacgctg gaaggcggcg
    24361 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgccgacgcg
    24421 gtgctgatta cgaccgctca cgcgtggcag catcagggaa aaaccttatt tatcagccgg
    24481 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtagcg
    24541 agcgatacac cgcatccggc tcggattggc ctgacctgcc agctggcgca ggtagcagaa
    24601 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
    24661 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
    24721 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
    24781 gacttccagt tcaatatcag tcgctacagc caacaacaac tgatggaaac cagccatcgc
    24841 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
    24901 ggtggcgacg actcctggag cccgtcagtt tcagcggaat tccagcttag cgccggtagc
    24961 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
    25021 gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt ttggatgttc
    25081 ggtttattct ttttctttta cttttttatc atgggggcct acttcccgtt tttcccgatt
    25141 tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt tgccgctatt
    25201 tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa actcgggctg
    25261 cgcaaatacc tgctgtggat tattaccggc atgttagtga tgtttgcgc
//
LOCUS       DAZRVY010000050        25106 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZRVY010000050 DAZRVY010000000
VERSION     DAZRVY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.050"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHXOX_002123"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHXOX_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR7005583.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHXOX_002124"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHXOX_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR7005584.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHXOX_002125"
     CDS             1145..1309
                     /locus_tag="ACHXOX_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR7005585.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHXOX_002126"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHXOX_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHXOX_002127"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHXOX_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR7005586.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHXOX_002128"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHXOX_002128"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR7005587.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHXOX_002129"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHXOX_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHXOX_002130"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHXOX_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR7005588.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHXOX_002131"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHXOX_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR7005589.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHXOX_002132"
     CDS             14459..15955
                     /locus_tag="ACHXOX_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR7005590.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHXOX_002133"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHXOX_002133"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR7005591.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHXOX_002134"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHXOX_002134"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR7005592.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHXOX_002135"
     CDS             18049..20109
                     /locus_tag="ACHXOX_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR7005593.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHXOX_002136"
     CDS             complement(20291..21613)
                     /locus_tag="ACHXOX_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005594.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHXOX_002137"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHXOX_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR7005595.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHXOX_002138"
     CDS             complement(23086..23985)
                     /locus_tag="ACHXOX_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7005596.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHXOX_002139"
     CDS             24505..>25106
                     /locus_tag="ACHXOX_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR7005597.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6991 g         5855 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttat
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggttttagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZRVY010000051        25064 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZRVY010000051 DAZRVY010000000
VERSION     DAZRVY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.051"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(166..576)
                     /locus_tag="ACHXOX_002140"
     CDS             complement(166..576)
                     /locus_tag="ACHXOX_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR7005598.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="ACHXOX_002141"
     CDS             complement(634..867)
                     /locus_tag="ACHXOX_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR7005599.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="ACHXOX_002142"
     CDS             1256..1801
                     /locus_tag="ACHXOX_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR7005600.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="ACHXOX_002143"
     CDS             1776..3701
                     /locus_tag="ACHXOX_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR7005601.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="ACHXOX_002144"
     CDS             3698..3904
                     /locus_tag="ACHXOX_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR7005602.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="ACHXOX_002145"
     CDS             3901..5502
                     /locus_tag="ACHXOX_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7005603.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="ACHXOX_002146"
     CDS             5483..6802
                     /locus_tag="ACHXOX_002146"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR7005604.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="ACHXOX_002147"
     CDS             6812..7144
                     /locus_tag="ACHXOX_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR7005605.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="ACHXOX_002148"
     CDS             7200..8225
                     /locus_tag="ACHXOX_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR7005606.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="ACHXOX_002149"
     CDS             8267..8665
                     /locus_tag="ACHXOX_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR7005607.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="ACHXOX_002150"
     CDS             8677..9030
                     /locus_tag="ACHXOX_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR7005608.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="ACHXOX_002151"
     CDS             9042..9620
                     /locus_tag="ACHXOX_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005609.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="ACHXOX_002152"
     CDS             9617..10012
                     /locus_tag="ACHXOX_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR7005610.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="ACHXOX_002153"
     CDS             10020..10760
                     /locus_tag="ACHXOX_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005611.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="ACHXOX_002154"
     CDS             10776..11198
                     /locus_tag="ACHXOX_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR7005612.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAIFHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="ACHXOX_002155"
     CDS             11180..11614
                     /locus_tag="ACHXOX_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR7005613.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="ACHXOX_002156"
     CDS             11607..14168
                     /locus_tag="ACHXOX_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7005614.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="ACHXOX_002157"
     CDS             14165..14494
                     /locus_tag="ACHXOX_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7005615.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="ACHXOX_002158"
     CDS             14494..15192
                     /locus_tag="ACHXOX_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR7005616.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="ACHXOX_002159"
     CDS             15198..15941
                     /locus_tag="ACHXOX_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7005617.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15938..16510
                     /locus_tag="ACHXOX_002160"
     CDS             15938..16510
                     /locus_tag="ACHXOX_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR7005618.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="ACHXOX_002161"
     CDS             16571..20068
                     /locus_tag="ACHXOX_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR7005619.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="ACHXOX_002162"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="ACHXOX_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..23803
                     /locus_tag="ACHXOX_002163"
     CDS             20720..23803
                     /locus_tag="ACHXOX_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7005620.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23803..24319
                     /locus_tag="ACHXOX_002164"
                     /pseudo
     CDS             23803..24319
                     /locus_tag="ACHXOX_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         6024 a         6185 c         7539 g         5316 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgcct
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttttc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctctgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gcgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcgcagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacatc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacatcgt ggcatctcgt
    23941 tgaagatcat cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt tatttatttc
    24001 tgaactcggc tcattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa
    24061 gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc ggatccggga
    24121 ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag cgccgcttca
    24181 ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg aagcctggaa
    24241 aaagtatcgg gtgttgctga accgtgttga tacatcaact gcaccggata ttgaatggcc
    24301 agtagcacct atagggtaaa ttcgtaatga ttacctaaat acgttattct tttgttaaaa
    24361 aagtgatttt gctataggta gggagtatgt tcttggatta agaataaaaa atcgccatga
    24421 aagagcttca tggcgagtac ataaacgtac aaaatgtgat gaaagtagag tggcgtaagc
    24481 cactatatta agatatgaca atagcgaaga aaagtaaata acattttatg gacgtttaat
    24541 tgttaattat aaaaactaga gtgcccgata ttgtttatct tgctttaaaa taatgtactc
    24601 aaaccaaatt tttgagctaa aagctcaaat aatataaatg caataataat gtttaataag
    24661 acaattagct tgctttgtat tttcatttcg gaatactgtt aagtaacttt gatgacccta
    24721 acaatagatc ggaattttct tagagtcaat ggtcactgtt tttgcttgca aagcaaatgt
    24781 tttggtggtt ataagttgtc ttaatatagt cacgcatgca acttgttcca tgcggagatt
    24841 aaagattgga atataacgcg aagatggaat taaattacat ttgcaataat caatttatta
    24901 ttactcaata attaccgata ttattattgt aaattaatca ttagagtgca aaaagaaatg
    24961 taacggcaaa ataagaggac gatagctgag aaaaaagcgt gctttcccaa tctggggcaa
    25021 atcacgtctg aatgacacct gaaaacaggt ggtagcctca agaa
//
LOCUS       DAZRVY010000052        24934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZRVY010000052 DAZRVY010000000
VERSION     DAZRVY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.052"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHXOX_002165"
     CDS             complement(<1..163)
                     /locus_tag="ACHXOX_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR7005621.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHXOX_002166"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHXOX_002166"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR7005622.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHXOX_002167"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHXOX_002167"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR7005623.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHXOX_002168"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHXOX_002168"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR7005624.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHXOX_002169"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHXOX_002169"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR7005625.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHXOX_002170"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHXOX_002170"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR7005626.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHXOX_002171"
     CDS             5058..6191
                     /locus_tag="ACHXOX_002171"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR7005627.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHXOX_002172"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHXOX_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR7005628.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHXOX_002173"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHXOX_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR7005629.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHXOX_002174"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHXOX_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR7005630.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHXOX_002175"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHXOX_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR7005631.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHXOX_002176"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHXOX_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR7005632.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHXOX_002177"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHXOX_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR7005633.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="ACHXOX_002178"
     CDS             9762..10631
                     /locus_tag="ACHXOX_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR7005634.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="ACHXOX_002179"
     CDS             complement(10834..11187)
                     /locus_tag="ACHXOX_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR7005635.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHXOX_002180"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHXOX_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR7005636.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="ACHXOX_002181"
     CDS             complement(13015..13308)
                     /locus_tag="ACHXOX_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR7005637.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHXOX_002182"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHXOX_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR7005638.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /locus_tag="ACHXOX_002183"
     CDS             complement(14862..15332)
                     /locus_tag="ACHXOX_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR7005639.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="ACHXOX_002184"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="ACHXOX_002184"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR7005640.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHXOX_002185"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHXOX_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR7005641.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="ACHXOX_002186"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="ACHXOX_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR7005642.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHXOX_002187"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHXOX_002187"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR7005643.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="ACHXOX_002188"
     CDS             20688..21263
                     /locus_tag="ACHXOX_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7005644.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="ACHXOX_002189"
     tRNA            21370..21445
                     /locus_tag="ACHXOX_002189"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            21688..21802
                     /locus_tag="ACHXOX_002190"
                     /pseudo
     CDS             21688..21802
                     /locus_tag="ACHXOX_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22062..22361
                     /locus_tag="ACHXOX_002191"
     CDS             22062..22361
                     /locus_tag="ACHXOX_002191"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR7005645.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHXOX_002192"
     CDS             complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHXOX_002192"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR7005646.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            22544..23599
                     /gene="cadC"
                     /locus_tag="ACHXOX_002193"
     CDS             22544..23599
                     /gene="cadC"
                     /locus_tag="ACHXOX_002193"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR7005647.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHXOX_002194"
     CDS             23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHXOX_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR7005648.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         6222 a         6267 c         6122 g         6323 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc
    23761 ttttcatgat atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta
    23821 cagaaacttt tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc
    23881 ttatcgccag tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc
    23941 aaaaatgaaa ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta
    24001 ccggtgttgt tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag
    24061 caagtatcgg tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc
    24121 tggcgtatgt atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt
    24181 atgccggaga aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta
    24241 actggattgg taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc
    24301 cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta
    24361 cctttgtaaa tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc
    24421 tggttcttat tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa
    24481 cttatgcagc taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta
    24541 ttctgctcta cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg
    24601 ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta
    24661 ttgtttacat cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg
    24721 cttccggtgc tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc
    24781 tggtttctgc attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg
    24841 taggccaggc aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag
    24901 tcgacagcaa cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZRVY010000053        24856 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZRVY010000053 DAZRVY010000000
VERSION     DAZRVY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.053"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHXOX_002195"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHXOX_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHXOX_002196"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHXOX_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR7005649.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHXOX_002197"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHXOX_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR7005650.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHXOX_002198"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHXOX_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR7005651.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHXOX_002199"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHXOX_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR7005652.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHXOX_002200"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHXOX_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR7005653.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHXOX_002201"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHXOX_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR7005654.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHXOX_002202"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHXOX_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR7005655.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHXOX_002203"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHXOX_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR7005656.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHXOX_002204"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHXOX_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR7005657.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHXOX_002205"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHXOX_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR7005658.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHXOX_002206"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHXOX_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR7005659.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHXOX_002207"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHXOX_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR7005660.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHXOX_002208"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHXOX_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR7005661.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHXOX_002209"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHXOX_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR7005662.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHXOX_002210"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHXOX_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR7005663.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHXOX_002211"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHXOX_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR7005664.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHXOX_002212"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHXOX_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR7005665.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHXOX_002213"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHXOX_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR7005666.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHXOX_002214"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHXOX_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR7005667.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHXOX_002215"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHXOX_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR7005668.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHXOX_002216"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHXOX_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR7005669.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHXOX_002217"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHXOX_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR7005670.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHXOX_002218"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHXOX_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR7005671.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHXOX_002219"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHXOX_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR7005672.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHXOX_002220"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHXOX_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR7005673.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHXOX_002221"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHXOX_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR7005674.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHXOX_002222"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHXOX_002222"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR7005675.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHXOX_002223"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHXOX_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR7005676.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHXOX_002224"
     CDS             14267..14635
                     /locus_tag="ACHXOX_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR7005677.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHXOX_002225"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHXOX_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR7005678.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHXOX_002226"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHXOX_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR7005679.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHXOX_002227"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHXOX_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR7005680.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHXOX_002228"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHXOX_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR7005681.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHXOX_002229"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHXOX_002229"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR7005682.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHXOX_002230"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHXOX_002230"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR7005683.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHXOX_002231"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHXOX_002231"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR7005684.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHXOX_002232"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHXOX_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR7005685.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHXOX_002233"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHXOX_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR7005686.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHXOX_002234"
     CDS             21814..22356
                     /locus_tag="ACHXOX_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR7005687.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHXOX_002235"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHXOX_002235"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR7005688.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHXOX_002236"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHXOX_002236"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR7005689.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHXOX_002237"
     CDS             23753..24010
                     /locus_tag="ACHXOX_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR7005690.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHXOX_002238"
     CDS             complement(23986..24540)
                     /locus_tag="ACHXOX_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR7005691.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6322 a         5903 c         6500 g         6131 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaag
//
LOCUS       DAZRVY010000054        24498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZRVY010000054 DAZRVY010000000
VERSION     DAZRVY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.054"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..228
                     /locus_tag="ACHXOX_002239"
     CDS             <1..228
                     /locus_tag="ACHXOX_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR7005692.1"
                     /translation="LYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAE
                     LLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            384..1919
                     /gene="gadC"
                     /locus_tag="ACHXOX_002240"
     CDS             384..1919
                     /gene="gadC"
                     /locus_tag="ACHXOX_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR7005693.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2050..3369
                     /locus_tag="ACHXOX_002241"
     CDS             2050..3369
                     /locus_tag="ACHXOX_002241"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR7005694.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3741..5123
                     /gene="dosC"
                     /locus_tag="ACHXOX_002242"
     CDS             3741..5123
                     /gene="dosC"
                     /locus_tag="ACHXOX_002242"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR7005695.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5148..7547
                     /gene="dosP"
                     /locus_tag="ACHXOX_002243"
                     /gene_synonym="pdeO"
     CDS             5148..7547
                     /gene="dosP"
                     /locus_tag="ACHXOX_002243"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR7005696.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHXOX_002244"
     CDS             7806..8387
                     /gene="ddpX"
                     /locus_tag="ACHXOX_002244"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR7005697.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8401..9951
                     /locus_tag="ACHXOX_002245"
     CDS             8401..9951
                     /locus_tag="ACHXOX_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7005698.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9953..10975
                     /locus_tag="ACHXOX_002246"
     CDS             9953..10975
                     /locus_tag="ACHXOX_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7005699.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHXOX_002247"
     CDS             10972..11868
                     /gene="ddpC"
                     /locus_tag="ACHXOX_002247"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR7005700.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11865..12851
                     /locus_tag="ACHXOX_002248"
     CDS             11865..12851
                     /locus_tag="ACHXOX_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7005701.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12880..13776
                     /locus_tag="ACHXOX_002249"
     CDS             12880..13776
                     /locus_tag="ACHXOX_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR7005702.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(13832..14263)
                     /gene="osmC"
                     /locus_tag="ACHXOX_002250"
     CDS             complement(13832..14263)
                     /gene="osmC"
                     /locus_tag="ACHXOX_002250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR7005703.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14608..14823
                     /gene="bdm"
                     /locus_tag="ACHXOX_002251"
     CDS             14608..14823
                     /gene="bdm"
                     /locus_tag="ACHXOX_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR7005704.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14925..15062
                     /gene="sra"
                     /locus_tag="ACHXOX_002252"
     CDS             14925..15062
                     /gene="sra"
                     /locus_tag="ACHXOX_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR7005705.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15219..16916
                     /gene="maeA"
                     /locus_tag="ACHXOX_002253"
     CDS             15219..16916
                     /gene="maeA"
                     /locus_tag="ACHXOX_002253"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR7005706.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17050..18052
                     /gene="adhP"
                     /locus_tag="ACHXOX_002254"
                     /pseudo
     CDS             17050..18052
                     /gene="adhP"
                     /locus_tag="ACHXOX_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18198..18482
                     /locus_tag="ACHXOX_002255"
     CDS             18198..18482
                     /locus_tag="ACHXOX_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR7005707.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18710..19363)
                     /gene="fdnI"
                     /locus_tag="ACHXOX_002256"
     CDS             complement(18710..19363)
                     /gene="fdnI"
                     /locus_tag="ACHXOX_002256"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR7005708.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19356..20240)
                     /gene="fdnH"
                     /locus_tag="ACHXOX_002257"
     CDS             complement(19356..20240)
                     /gene="fdnH"
                     /locus_tag="ACHXOX_002257"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR7005709.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20253..23300)
                     /gene="fdnG"
                     /locus_tag="ACHXOX_002258"
     CDS             complement(20253..23300)
                     /gene="fdnG"
                     /locus_tag="ACHXOX_002258"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22713..22715),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR7005710.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGGGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23532..24413
                     /gene="yddG"
                     /locus_tag="ACHXOX_002259"
     CDS             23532..24413
                     /gene="yddG"
                     /locus_tag="ACHXOX_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR7005711.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6013 a         6215 c         6190 g         6080 t
ORIGIN      
        1 ctgtacgacc tctctgaacg tctgcgtctg cgcggctggc aggttccggc cttcactctc
       61 ggcggtgaag ccaccgacat cgtggtgatg cgcattatgt gtcgtcgcgg cttcgaaatg
      121 gactttgctg aactgttgct ggaagactac aaagcctccc tgaaatatct cagcgatcac
      181 ccgaaactgc agggtattgc ccagcagaac agctttaaac atacctgata acgtttaacg
      241 gtaacggtgt cccgaaacga acccgtttcg ggataatttc caaagtctgt tcactggcat
      301 tagcaacgga aaatattgtt ctgaatacgc ttcagaacaa aacaggtgcg gttccgacag
      361 gaataccgtt ttagggggat aatatggcta catcagtaca gacaggtaaa gctaagcagc
      421 tcacattact cggctttttt gccataacgg catcgatggt aatggctgtt tatgaatacc
      481 ctaccttcgc aacatcgggc ttttcattag tcttcttcct gctattaggc gggattttat
      541 ggtttattcc cgtgggactt tgtgctgcgg aaatggccac cgtcgacggc tgggaagaag
      601 gtggtgtctt cgcctgggta tcaaatactc tggggccgag atggggattt gcagcgatct
      661 catttggcta tctgcaaatc gccattggtt ttattccgat gctctatttc gtgttagggg
      721 cactctccta catcctgaaa tggccagcgc tgaatgaaga ccccattacc aaaactattg
      781 cagcactcat cattctttgg gcgctggcat taacgcagtt tggtggcacg aaatacacgg
      841 cacgaattgc taaagttggc ttcttcgccg gtatcctgtt acctgcattt attttgatcg
      901 cattagcggc tatttatctg cactccggtg cccccgttgc tatcgaaatg gattcaaaga
      961 ccttcttccc tgacttctct aaagtgggca ccctggttgt atttgttgcc ttcattttga
     1021 gttatatggg cgtagaagca tccgcaaccc acgtcaatga aatgagcaac ccagggcgcg
     1081 actatccgtt ggctatgtta ctgctgatgg tagcggcaat ctgcttaagc tctgttggtg
     1141 gtttgtctat tgcgatggtc attccgggta atgaaatcaa cctctccgca ggggtaatgc
     1201 aaacctttac cgttctgatg tcccatgtgg caccggaaat tgagtggacg gttcgcgtga
     1261 tctccgcact gctgttgctg ggtgttctgg cggaaatcgc ctcctggatt gttggtcctt
     1321 ctcgcgggat gtatgtcaca gcgcagaaaa acctgctgcc agcggcattc gctaaaatga
     1381 acaaaaatgg cgtaccggta acgctggtca tttcacagct ggtgattact tctatcgcgt
     1441 tgatcatcct caccaatacc ggtggcggta acaacatgtc cttcctgatc gcactggcgc
     1501 tgacggtggt gatttatctg tgtgcttatt tcatgctgtt tattggctac attgtgttgg
     1561 ttcttaaaca tcctgactta aaacgcacat ttaatatccc tggtggtaaa ggggtgaaac
     1621 tggtcgtggc tattgtcggt ctgctgactt caattatggc gtttattgtt tccttcctgc
     1681 cgccggataa catccagggt gactctaccg atatgtatgt tgaattactg gtcgttagtt
     1741 tcctggtggt acttgccctg ccctttattc tctatgctgt tcatgatcgt aaaggcaaag
     1801 cgaataccgg cgtcactctg gagccaatca acagtcagaa cgcaccaaaa ggtcacttct
     1861 tcctgcaccc gcgtgcacgt tcaccacact atattgtgat gaatgacaag aaacactaac
     1921 catgtctgaa tggttataag acaagggagc gataattcat cgctcccttt ttcgtgctcg
     1981 ctgtcgtatt gacttaaccg ggataagtac gagaatgagc gcacatctgt ttaccggaaa
     2041 ccagcatata tggatatctg ctcccgaaac gagaaattag cgattagaag accagcgata
     2101 ctggttgcac tggcactttt actgtgtagt tgtaaaagca cgcctccaga gtccatggtg
     2161 acaccaccag caggttcaaa gccaccagcc acgacgcaac aatcgtcaca accgatgcgt
     2221 ggcatctggc tggccacggt gtctcgcctc gactggccac cggtttcctc ggttaacatt
     2281 agtaacccca ccagccgggc ccgtgtacaa caacaggcga tgatcgacaa actggatcat
     2341 ctgcaacgtc tcggtataaa cacggtcttt ttccaggtta agccggacgg taccgccctg
     2401 tggccatcga aaattttgcc atggtccgat cttatgaccg gtaagattgg tgaaaatccg
     2461 ggttacgatc cgctgcaatt tatgctcgac gaagcccaca agcgtgggat gaaagtacac
     2521 gcctggttta acccctatcg cgtatcggtt aatacgaagc ccggtactat cagggaactg
     2581 aatagcactc tgtctcaaca accggcgagc gtctatgtgc aacaccgtga ctggatcaga
     2641 acgtctggcg atcgctttgt cctcgacccg ggcatccctg aggttcagga ctggatcaca
     2701 tcaatagtcg cagaagtggt ttcccgctat ccggtagatg gcgtgcagtt tgacgactat
     2761 ttctatactg agtcaccggg ttcacggcta aatgataacg aaacgtaccg caaatacgga
     2821 ggcgcatttg cgtcaaaagc agactggcgg cgcaacaata ctcagcagtt aattgcaaaa
     2881 gtatcgcaca ccattaaaag cattaagccg ggagtcgaat ttggcgttag cccggcaggc
     2941 gtgtggcgta accgatcaca cgatccgctc ggttccgata cccgaggcgc ggcagcctat
     3001 gacgaatcct acgctgacac ccgtcgatgg gtggaacaag gattgctgga ttacattgct
     3061 ccccaaattt actggccgtt ctcacggagt gccgcgcgtt atgacgtgtt ggcaaaatgg
     3121 tgggcggatg tcgttaaacc gaccaggacc cgcctgtata tcggtatcgc cttctataaa
     3181 gtgggtgaac cttcaaagat agagccagac tggatgatta acggcggcgt accggaactg
     3241 aaaaagcagc tcgatcttaa cgatgcggta ccagaaatta gcggcaccat cttgttccgt
     3301 gaggactatc tgaataaacc gcagactcaa caagcggtca gctatctgca aagtcgttgg
     3361 ggcagttaaa ctcccatgct tactttgtaa caagccggtg ctttacccac cggcttgtta
     3421 caccttgtga aatatcccac tagtaatcat gcttacataa gtcaaattaa ccacgcaatt
     3481 aaccacactt agttaaacag ctataaatcc ctaataaaaa tgtatgaata tgaaaaacca
     3541 tacgatattc gctgtcaccg aaaaataatt acgatgggct atactcttct gaagaacagt
     3601 agcgtagatt gaaactgtcc taagtcgctt atttcttttc agtatatctt catatttcag
     3661 gagaatatgg tgcagttagt caatctcgca cgttgcgttt tgattccgtc tgatttccca
     3721 gccttataag ggtgtgaatt atggagatgt attttaaaag aatgaaagat gagtggaccg
     3781 gacttgtcga acaggcagat ccactcattc gtgctaaagc cgcggaaatt gccgttgcgc
     3841 atgctcatta tctgagtatc gagttttatc gaattgtccg catcgacccg catgccgaag
     3901 aattcttgag taatgaacaa gttgagcggc agttgaaaag tgcgatggaa cgctggatta
     3961 ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct aatacaaatc cagcataccg
     4021 tcgcggaagt gcatgcccgc ataggaattc cggtagaaat tgtcgagatg gggtttcggg
     4081 tgctgaaaaa aatcctctat ccggtaatct tctcttcgga ttattccgcc gcagaaaaac
     4141 ttcaggtcta tcatttctcg attaacagta ttgatatcgc gatggaagtg atgacccgcg
     4201 cgtttacctt tagtgacagt agtgcctcaa aggaagatga aaattatcgt atcttctcgt
     4261 tactggaaaa cgccgaagaa gaaaaagaac ggcaaatagc ctcaatactt tcatgggaaa
     4321 tagatattat ctataaaatc ctgctggatt ctgatttagg cagtagtttg ccattaagcc
     4381 aggctgattt tggcctgtgg tttaaccata aaggtcgaca ttattttagt ggcattgccg
     4441 aagtaggcca tatctcccgt ctcattcagg atttcgacgg tattttcaat caaaccatgc
     4501 gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa atttttatta cagataagaa
     4561 ataccgtatc gcagattatt accttgctgc gtgaattgtt tgaagaagta tcgcgccacg
     4621 aagtcggtat ggatgtacta acgaaattac ttaaccgccg tttcctgccg actatcttca
     4681 aacgcgaaat tgcccatgcc aaccggaccg gtacaccgct gtcagtgctg attattgacg
     4741 ttgataaatt caaagagatc aacgatacgt ggggccataa cactggcgat gaaattctgc
     4801 gtaaagtctc tcaggccttt tatgacaacg tccacagtag tgattatgtt tttcgctacg
     4861 ggggcgatga atttatcatt gttttgactg aagcttctga aaacgaaacg ttacgtaccg
     4921 cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa agccgcaaac ggcgaggata
     4981 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     5041 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     5101 aactctggaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     5161 atgcggaaaa tgccgccgat ggcatttttt tccccgccct tgaacaaaat atgatgggcg
     5221 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     5281 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccgcgggatt
     5341 tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga aggtggtaaa gcgcgcgtcg
     5401 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     5461 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     5521 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     5581 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     5641 ttaccgaaat gtttggttac tgcattagcg aagccagcgg tatgcagccc gatacactcc
     5701 tgaacattcc tgaattccct gccgataacc gcattcgttt acaacagttg ctatggaaaa
     5761 ccgcccgcga tcaggacgaa tttctgctgt tgacacgcac cggtgaaaaa atctggatta
     5821 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacct
     5881 tctcggatat caccgaagaa cggcagatcc gccagcttga aggcaatatt ctcgccgcca
     5941 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     6001 ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa cgggatgccg atacactggg
     6061 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     6121 agcgtgatgg tgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     6181 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     6241 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     6301 caaatcgcaa taacctgcac aattacctcg atgaccttgt cgacaaagcc gtctctccgg
     6361 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     6421 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     6481 agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt gagcctggaa aacgacgtca
     6541 gtaacattac ccaaatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     6601 acgataaacc attcccgcta accttgagta ttggcatcag ctacgacgtg ggtaaaaacc
     6661 gcgattacct gctctccact gctcacaatg caatggatta tattcgcaag aatggcggta
     6721 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     6781 gcgcagcgct gaaagaagcg attagcaata accaactgaa actggtttac cagccgcaaa
     6841 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg cacgatcccc
     6901 agcatggtca tgtaccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6961 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     7021 agaatattca tatcccggcg ttatccgtga acttgtcggc gctgcacttt cgcagtaatc
     7081 agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg tattgacggc caccagctga
     7141 cggtagaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     7201 agatcctgcg cgatatgggc gtgggcttat cggtagatga ttttggcacg ggcttttccg
     7261 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgacaaa agttttgtcg
     7321 atcgttgtct gacggaaaaa cgcatccttg ccttacttga agccattacc agcattgggc
     7381 aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     7441 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctacccgccg
     7501 aggaaattcc aggctggatg agcagcgtgt taccgctgaa aatctgacaa attcctctcg
     7561 cccgcactcg cgggttttca tttaacgtga cactgtcact taaatactgt gatttcagct
     7621 acgattccgg gagattcctt ccttaacttc ccctcttgca ccaaaactgc actacgctgc
     7681 acaaatgttg atcaaaagtt aaaaaatata actttaagcc agcattctgg cgcttatccc
     7741 ggcatggcat gagatctgca taagcggaaa gcgcagcaat ttttgtctta atacaggaac
     7801 cgtttatgtc ggataccacc gaactggttg atttagccgt gatcttccct gatctggaga
     7861 tcgaattgaa atacgcctgc gctgataaca tcacaggtaa agctatttac cagcaagcgc
     7921 gttgtctgtt acacaaggat gcgattaccg cgctggcgaa aagtatcagt atcgcccagc
     7981 tgtcaggctt acaactggtg atttacgatg cgtatcgccc acaacaagca caggcgatgt
     8041 tgtggcaagc ctgcccagac ccgcaatatg ttgttgatgt gacggtcggt tctaatcaca
     8101 gccgtggcac ggcgatcgac ctgacgcttc gtgatgagca cgggaacatc ctggatatgg
     8161 gcgcagggtt cgatgaaatg cacgagcgct cccatgccta tcacccttcc gtcccgcccg
     8221 ccgctcagcg caatcgcctg ttgctgaatg cgataatgac tggtggcggt tttgtcggta
     8281 tctccagcga atggtggcac ttcgaattac cccaggcagc gagttaccct ctgctcgctg
     8341 atcaattctc ttgttttata tcccctggca cacagcacgt cagttaatca ggaaactgtc
     8401 atgaagagat cgatatcgtt tcgtcccaca ttgctcgcgc tcgtccttgc cacaactttc
     8461 ccggttgcgc acgccgccgt accgaaagat atgctggtga ttggtaaggc cgccgatcca
     8521 caaaccctcg acccggcggt aacaatagat aataacgact ggacagtgac ctacccgtct
     8581 tatcagcggc tggttcagta caaaacggac ggtgataaag gctcaaccga cgttgaaggc
     8641 gatctggcaa gtagctggaa agcgtctgac gatcaaaaag agtggacgtt caccctgaag
     8701 gacaatgcta aatttgccga tggcacacct gtcactgccg aagcagtaaa actctctttt
     8761 gagcggttac taaaaatcgg ccaggggcca gcagaagcat ttcccaaaga tttaaagatt
     8821 gatactcccg acgaacatac ggtgaagttt acccttagcc aaccattcgc accgttcctc
     8881 tacacgctag cgaatgacgg tgcatccatt atcaatccgg cggtcttaaa ggaacatgca
     8941 gcggatgatg ctcgcggctt cctcgcgcaa aataccgccg gttccggacc atttatgctg
     9001 aaaagctggc aaaaaggtca gcaattagtt ctggtgccaa atccgcatta ccccggcaat
     9061 aaaccgaact ttaagcgagt atcggtaaaa attatcggtg aaagtgcctc ccgtcgcctg
     9121 cagctctccc gtggcgacat tgacattgcc gatgcgctgc cggtggatca actcaacgcc
     9181 ctgaagcagg aaaataaagt caatgtggca gagtatccgt cactgcgcgt tacctatctg
     9241 tatctgaata acagcaaagc gcctcttaat caggcggatc tgcgtcgggc catttcctgg
     9301 tctaccgatt atcagggcat ggttaacggc attctgagtg gtaacggaaa acagatgcgc
     9361 ggcccgattc cggaaggcat gtggggctac gatgcgacgg caatgcaata caaccatgac
     9421 gaaacgaaag ccaaagccga atgggataaa gtgacgagca aacccaccag cctgacgttt
     9481 ctctactccg ataacgatcc gaactgggag cctattgctc tggcgacaca atccagtctc
     9541 aacaagctgg gcatcaatgt gaagctggaa aagctggcga acgccaccat gcgcgacaga
     9601 gtgggtaaag gtgattacga cattgcgatt ggcaactgga gtccggattt tgccgacccg
     9661 tatatgttta tgaattactg gtttgagtca gacaaaaaag gtctgccggg taaccgctcg
     9721 ttctatgaaa acagtgaggt cgataagtta ctgcgcaatg cgcttgcgac caccgaccag
     9781 acgcagcgta cccgggacta ccagcaggca cagaaaatcg tcattgatga cgctgcttat
     9841 gtgtacctgt tccagaaaaa ctaccaactg gcgatgaaca aagaggtgaa aggctttgtg
     9901 ttcaatccca tgctggaaca ggtcttcaat atcaatacca tgagtaaata acgtgacctt
     9961 ctggagtatt ttacgccaac gctgctgggg gctggtgctc gtggtggcgg gcgtctgcgt
    10021 gattacgttt attatctcgc atctgatccc tggcgatccg gcgcggttac tggcgggtga
    10081 ccgcgccagc gatgctatcg tggaaaatat tcgccagcaa ctgggactgg accagccact
    10141 gtatgtacag ttttaccgct acttcagcga cctgtttcat ggtgacctgg gaacatccat
    10201 tcgtaccggg cgtccggtgc tggaagagtt gcgtatattt ttcccggcga cgctggaact
    10261 ggctttttgt gccctgctgc tggcactcct gattggcatc ccgctgggca tactctctgc
    10321 agtctggcga aatcgctggc tggatcatct ggtgcgaata atggccatta ccggaatctc
    10381 cacacctgcg ttctggcttg gactgggcgt cattgtgctg ttttatggtc atctgcaaat
    10441 tcttcccggc ggcggaaggc ttgatgactg gctggatcca ccaacgcacg ttaccggctt
    10501 ttatctgctc gatgcgctgc ttgaaggcaa cggtgaagtc ttcttcaatg cgttgcaaca
    10561 tctcatctta ccggcattaa cgctggcgtt cgttcacctg ggaattgtcg ctcgccagat
    10621 ccgctcagcg atgctggaac aattgagtga agactacatt cgtaccgccc gggccagcgg
    10681 cttgcccggc tggtatatcg ttttatgtta tgcgctaccc aatgcgttga tcccatcgat
    10741 taccgtattg ggtctggcgc tgggcgattt gttgtatggc gcagtgctca ccgaaaccgt
    10801 ttttgcctgg cccggaatgg gtgcatgggt agtaacatca atacaggcgc tcgacttccc
    10861 ggcagtgatg ggctttgccg tcgtggtttc atttgcttat gtgctggtca acctggtggt
    10921 ggatttgctc tatttgtgga ttgatccgcg tatcggacgt ggaggtggtg aatgatgcta
    10981 agcgaggaaa cgtccgccgt acgcccacaa aaacaaacgc gatttaacgg tgcaaaactg
    11041 gtatggatgc tgaaaggcag tccgctcacc gtgaccggcg cagtcatcat tgtattaatg
    11101 ctattgatga tgattttttc accgtggctt gcgacgcatg atcccaacgc cattgattta
    11161 accgcccgcc ttttgccgcc ttctgcggcg cactggtttg gcaccgatga agtgggacgc
    11221 gatctgttca gccgcgtact ggtcggcagt cagcaatcaa ttctcgccgg attagtcgtg
    11281 gttgccattg cgggtatgat tggttcgcta ctcggatgtc tatccggtgt gcttggcgga
    11341 cgcgcagacg ccatcatcat gcgcatcatg gacattatgc tgtcgattcc ttcgttggta
    11401 ctgacaatgg cactggctgc cgctctcggg ccgagtttat ttaacgccat gctggcgatt
    11461 gctattgtgc gaattccctt ttatgtgcgc ctggcgcggg ggcaagcatt agttgtacgc
    11521 cagtatacct atgttcaggc ggcgaaaacc tttggtgcgt cgcgttggca tctgatcagc
    11581 tggcatattt tacgtaactc cctgccgccg ctgatcgtgc aggcatcgct ggatatcggt
    11641 agcgcgattt taatggccgc cacgttggga tttattggcc tgggtgctca acaaccgagt
    11701 gctgaatggg gcgcgatggt agctaatggt cgcaactatg tactcgatca gtggtggtat
    11761 tgcgcatttc cgggggcagc gattttgctt accgccgtcg ggtttaatct ctttggtgat
    11821 ggtattcgcg atctgcttga cccgaaagca ggaggaaagc agtcatgacc caacccgttc
    11881 tggacattca acagctgcat ttgagtttcc ccggttttaa cggttatgtt cacgtgctca
    11941 acaatgtgtc cttgcagatt aaccgcggtg aaattgtcgg tctggtggga gaatccggct
    12001 caggtaaatc agtcaccgca atgctgatta tgcgtctgct accgacgggc agttattgcg
    12061 tacatcgggg gcagatttca ctgctgggag acgatgttct taacgctcgg gagaaacagc
    12121 ttcgtcagtg gcgcggcgca cgagtggcga tgatctttca ggaaccgatg accgccctca
    12181 atccgacacg tcgaataggt cttcagatga tggacgtgat ccgccatcat caaccaataa
    12241 gtcgtcggga agccagagct aaagcgatta ccctgctgga agagatgcaa atcccggatg
    12301 ccgtggaagt tatgtcgcgc tatccgtttg agctttcagg tggtatgcgc cagcgggtaa
    12361 tgattgcgct ggcattctcc tgcgagccgc aattgattat tgccgacgaa ccgactacgg
    12421 cgctggacgt cacggtacag ttgcaggtac tgcgtttgct taaacataaa gctcgcgcca
    12481 gtggaactgc ggtactgttc atcagccatg atatggccgt ggtgtcgcaa ctgtgcgata
    12541 gcgtttacgt gatgtatgcc ggaagcgtaa ttgaaagcgg cgtgacggca gacgttatcc
    12601 atcatccccg gcatccgtat accattggtt tgctgcaatg cgcaccagaa catggagtac
    12661 cacgccagcc attacccgcc attccaggga cggtaccaaa cctcacccat ttgcctgacg
    12721 gctgcgcttt tcgcgatcgt tgctatgcgg caggtgcaca gtgtgaaaac gtcccggcgc
    12781 tgacagcgtg tggtgacaac aaccagcgct gcgcctgttg gtatccgcag caggaggtca
    12841 taagtatctg acacgttatt aacgttacgc gacgtccata tcaatttccc ggcccgtaaa
    12901 agctggcttg gtaaaactac ggaacatgtt catgcgatta atggtattga tttacagatc
    12961 cgccgtggtg aaaccttagg gatcgtcggt gagtcaggct gcggcaaaag caccctcgca
    13021 cagcttttaa tgggtatgct gcaaccgagc cacgggcaat acatccgttc aggatcacaa
    13081 cgcattatgc agatggtgtt tcaggacccg ctctcttcgc ttaatccgcg cttatcggtg
    13141 tggcgcatca tcacagaacc gctctggata gctaagcgta gtagtgaaca acagcggcga
    13201 gcgttggcaa aggagttggc tgtgcaggtg ggtattcgtc cggagtatct cgaccgcctg
    13261 cctcatgcgt tctccggcgg gcagcggcaa cgcatcgcca ttgccattgc cagagcactc
    13321 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
    13381 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
    13441 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
    13501 ctcgggcaga ttgtagaact gggcgacgcg cagcaggtgc tgacggcacc tgcacatcca
    13561 tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
    13621 gcattatgta aaacggatct gccgggaaac cgcacgttgc cacaaggctg ttttttctac
    13681 gaacgttgcc ctctggcaac ccacggatgt gaagtccggc aatcattagc aataagggag
    13741 gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
    13801 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tccagcgtaa
    13861 tttccgcttt cagtacctga gaaaccgggc atcctgcttt tgctttctgg atgatgccgt
    13921 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
    13981 tcgcaaaatc ggcatccact ttatccagcg acacatcggc ggtggtatca atcgatgttg
    14041 gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
    14101 ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc
    14161 catacggctg ttggttcagc acgccactct gggtggatac tgttcccttc ccgcgtttga
    14221 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
    14281 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
    14341 ataaaatacg gcgaaaatat tacttctgca cttaaattaa attcccacaa tagataaccg
    14401 ggaatatata tctcaagaga tattgcatca atatcaatca actcaccaca ataataatac
    14461 atatcaatat catggagtta aaatataaaa atcatcaacc aggactaatc ttaacaacga
    14521 agcggcaaat atttgccatg ctgaatgtgc tttataaatc tgcgatccgt agcagacacc
    14581 ataaatacac agacacggag aatcactatg tttacttatt atcaggcaga aaattcaaca
    14641 gcagaacccg ctctggttaa tgccattgag caaggccttc gggcagagca aggtgttgtc
    14701 actgaagatg acattctcat ggagctgacc aaatgggtgg aagcttctga taacgacatc
    14761 ctcagtgata tctaccagca aacgatcaat tatgtggtca gtggccagca ccctacgctt
    14821 taaggtgcta tgcttgatcg gcaacctaat ttaggggttt agcacgtgtt tcttcgctac
    14881 ggcgatgttg tccttaaaac tagcaacagg attgaggagt taaaatgaaa tcgaaccgtc
    14941 aggcacgtca tattcttgga ctggaccata aaatttctaa ccagcgcaaa atagttaccg
    15001 aaggtgacaa atccagcgtg gtaaataacc caaccggcag aaaacgcccc gctgaaaagt
    15061 aattcataac catcagtcct caatgacgat taaacaccat tgcctgcgca atggtgtttt
    15121 tgtttttatc tgctttatac ttggggccga cgccctggcg gtaaagcaaa gacgataaaa
    15181 gcgtgccagg gatggatatt caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac
    15241 agcgttcgct ttatatccct tacgctggcc ctgtactgct ggaatttccg ttgttgaata
    15301 aaggcagtgc cttcagcatg gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg
    15361 aagtggtcga aaccatcgaa gaacaagcgg aacgagcatg gatccagtat cagggattca
    15421 aaaccgaaat cgacaaacac atctacctgc gtaacatcca ggacaccaac gaaaccctct
    15481 tctaccgtct ggtaaacaat catcttgatg agatgatgcc tgttatttat accccaaccg
    15541 tcggcgcagc ctgtgagcgt ttttctgaga tctaccgccg ttcacgcggc gtgtttatct
    15601 cttaccagaa ccgccacaat atggacgata ttctgcaaaa cgtaccgaac cataatatca
    15661 aagtgattgt ggtgactgac ggtgaacgta ttctggggct tggtgaccag ggcatcggcg
    15721 ggatgggcat tccgatcggt aaactgtcgc tctataccgc ctgtggcggc atcagcccgg
    15781 cgtataccct tccggtggtg ctggatgtcg gaacgaacaa ccaacaactg cttaacgatc
    15841 cgctgtatat gggctggcgt aatccgcgta tcactgacga cgaatactat gaattcgttg
    15901 atgaatttat ccaggctgtg aaacaacgct ggccagacgt gctgttgcag tttgaagact
    15961 ttgctcaaaa aaatgcgatg ccgttactta accgctatcg caatgaaatt tgttctttta
    16021 acgatgacat tcagggcact gcggcggtaa cagtcggcac actgatcgca gcaagccgag
    16081 cggcaggtgg tcagttaagc gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat
    16141 gcggcattgc cgaaatgatc atcgcccaga cccagcgcga aggattaagc gaggaagcgg
    16201 cgcggcagaa agtctttatg gtcgatcgct ttggcctgct gacggacaag atgccgaacc
    16261 tgctgccttt ccagaccaaa ctggtgcaga aacgcgaaaa cctcagtgac tgggataccg
    16321 acagcgatgt gctgtcactg ctggatgtgg tgcgcaatgt aaaaccagat attctgattg
    16381 gcgtctcagg acagaccggg ctgtttacgg aagagatcat ccgtgagatg cataaacact
    16441 gtccgcgtcc gatcgtgatg ccgctgtcta acccgacgtc acgcgtggaa gccacaccgc
    16501 aggacattat cgcctggacc gaaggtaacg cgctggtcgc cacgggcagc ccgtttaatc
    16561 cagtggtatg gaaagataaa atctacccta tcgcccagtg taacaacgcc tttattttcc
    16621 cgggcatcgg cctgggggtt attgcttccg gcgcgtcacg tatcaccgat gagatgctga
    16681 tgtcggcaag tgaaacgctt gcacagtatt cgccgctggt gctgaacggc gaaggtctgg
    16741 tactaccgga actaaaagat attcagaaag tctcccgtgc aattgcgttt gcggttggca
    16801 aaatggcgca gcagcaaggc gtggcggtga aaacctctgc cgaagctttg caacaagcca
    16861 ttgacgataa tttctggcaa gccgaatacc gcgactaccg ccgtacctcc atctaagcct
    16921 gcgcccggta gtgaaggcta ccgggcaatt ttcctctccc tttttcagat ctcatccata
    16981 ctgggtagtg gcgaataaat ctcatttgcc tcacctgcta tgcagaacat catccgaaaa
    17041 ggaggaacta tgaaggctgc agttgttacg aaggatcatc atgttgacgt tacggataaa
    17101 acactgcgct cactgaaaca tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt
    17161 cataccgatc ttcatgttaa gaatggcgat tttggtgaca aaaccgacgt aattctgggc
    17221 catgaaggca tcggtgtggt ggcagaagtg ggtccaggtg tcacctcatt aaaaccaggc
    17281 gatcgtgcca gcgtggcgtg gttctacgaa ggatgcggtc attgcgaata ctgtaacagt
    17341 ggtaacgaaa cgctctgccg ttcagttaaa aatgccggat acagcgttga tggcggaaga
    17401 gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact cggcggcggc
    17461 cagcagcatt acctgtgcgg gggtcaccac ctacaaagcc gttaagctgt caaaaattcg
    17521 tcccgggcag tggattgcta tctacggtct tggcggtctg ggtaacctcg ccctgcaata
    17581 cgcaaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg agcagttaaa
    17641 actggcaacc gaaatgggtg cagatttagc gattaactca cgcaccgaag acgccgccaa
    17701 aattgtgcag gagaaaaccg gtggcgctca ctctgcggtg gtaacagcag tagctaaagc
    17761 tgcgtttaac tcggcagttg atgctgtccg tgcaggcggt cgtgttgtgg ctgtcggtct
    17821 gccgccggag tctatgagcc tggatatccc acgtcttgtg ctggatggca ttgaagtggt
    17881 cggttcgctg gtcggcacgc gccaggatct aaccgaagcc ttccagtttg ccgccgaagg
    17941 caaagtggtg ccgaaagtcg ccctgcgtcc gttagcggac atcaacacca tctttaccga
    18001 gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcact aagaggtctt
    18061 tgctgcgact cccatgttcg ggtcgcagca tcgcacactc tccaacatga aatggctaaa
    18121 tggattattg ttgcgtagga aggaacaata atgatagtgt tcccagagaa ggaacgctac
    18181 tgagagtaaa gacactaatg aaaatggcca atcatccccg cccgggggac attattcagg
    18241 aatcactgga cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc
    18301 cctcaacggc aagccgattg ctgaccggaa aagcagcttt gacgccagaa atggcaatta
    18361 aactttccgt ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga
    18421 gtctggcaga ggcagaaaaa acggtggatg tgtcgcgatt acgccgtctg gttacgcaat
    18481 aaaaaatggc accttataaa gcgccatttg aaactgatga caaacgcaac attgcctgat
    18541 gcgctacgct tatcaggcct acacgttcac ttcaatctat tgtatttgaa gggttttcat
    18601 gccggataag gcattcacga cgcatccggg atgaacaaag cccacgttgt ctacaatatg
    18661 aaaatggcgc cttttacagc gccattttca gcttaaagtt caaaagagat tatatccctt
    18721 cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc ggatggtgtt
    18781 tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac cctttcaccc
    18841 agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc gcagccgcgt
    18901 ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag tacggacgcc
    18961 agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac cagaacatca
    19021 tcttttgccc ggcgttgtac ttaccaacat ccgccacttt gtgctcattg ccttttaata
    19081 cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg tgatgcacaa
    19141 aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaagaac gggtgcaaaa
    19201 tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc gggaagaaga
    19261 acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag tgacaggcgc
    19321 gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc ctcctcgtca
    19381 tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc ggcaaaagtg
    19441 gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct taccgaggtg
    19501 tcgatcttcg gatctttcgg cagaccgtaa tacagctccg gctgatcggc gtgatgcagc
    19561 acgtacataa cgtgcgtacc accgacccct ttcgggttgt agacgccagc atgttcgtaa
    19621 ccacgcgctt tcagtttcgc cacgcgctct tccgccagct ccagcatctc cttcttggtg
    19681 ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg gccgacgctg
    19741 acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt gaggcgcgga
    19801 atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc cgactggaaa
    19861 tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc cttcaggcag
    19921 ccgggatctt cacagtgcat acagccgtct ttacggatca gccactccag cttgccgttc
    19981 tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc ggcggggtta
    20041 tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc cgaacacgcc
    20101 acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagttttgc gacttctgct
    20161 ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct tttgataata
    20221 tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt gactaagaac
    20281 gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag agtgttagcg
    20341 atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc caccgtttca
    20401 acctgctgac cgtttacatt cagcggtttc agacggcgcg ttaccaccgc caccgcgcgg
    20461 ataaagccac gcttgcttga gacagtgaca cgatcgccat tattaatgcc tttcgccgcc
    20521 gccagcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt gagcaatgcg
    20581 tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac atacgggaac
    20641 tgctcttttt tacccatccg cagcgcgtct tgttcataca gacgaacaac cgggttagac
    20701 accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc gtagtgttcc
    20761 gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc ttccggctgc
    20821 atgataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtccgg aatatcgtta
    20881 cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca cggtttacca
    20941 ttgatatccg ccgaagcacg gttgtagagc acgcgacggt tgagcggcca cgcccaggcc
    21001 catcccagcg tattccccag accggatggg tcggagttat cgcggttagc catctggttg
    21061 ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt accgtcatca
    21121 cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac gccattagcg
    21181 tcatagagat cttccagcgc atagccgtta ttctctttcg ccacttcgtc agattgcggt
    21241 tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc tttaccacct
    21301 tcggcctggt acagctcgcg cagatgatgg tagatacccg ccagaatttc gccgtcgtta
    21361 cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact gcaaccagcg accggagtta
    21421 gcgatagaac catcttcttc ggcaaagcag gtcgaaggca gacggaatac ttcagtctga
    21481 atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt agaggtttca
    21541 gtcaccagcg gatcgataac caccatgtac ttcagcttgc tcaggcagct caccactttg
    21601 tttttgtccg ggaaggacgc aaccgggtta aagccctggc agaagtaacc ggtgactttg
    21661 ccttcgtcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca cttcggcagc
    21721 cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga tttcatcagg
    21781 ctaacgaaga acttcggata gttgctccag tagttcacct gatcagccag cgtcgctttc
    21841 ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg aagcgtcaga
    21901 taacctggca ggctggtaga gagcagacct aagtcagtca gcccctgaat gttggagtga
    21961 ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag caactggatc
    22021 atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc cagcgcgtac
    22081 aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca cactttcagg
    22141 aagtcggctt ttggcgtacc gcagatgttt tcaacgacgt ccggcgtgta gcgggaaacg
    22201 tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc gcgttttgca
    22261 tagccgtttt catcgaactg atagttccag gacgacttat catactggcg tttttcagcg
    22321 tcgtagccgc tgaacaggcc gtcttcgaaa gcaaaatcat cacgcaccag caggctggcg
    22381 ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag gtagcgcaaa
    22441 acgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat atccgccaca
    22501 gaagcggtac gcgtaaaacg aggatcgaca acgatcaagg ttgcatcgtt gttgtttttc
    22561 gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc gcccattacc
    22621 attacgacgt tagcgttttt gatatccacc cagtggttgg tcatcgcacc gcgaccaaat
    22681 gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt gtctaccgcc
    22741 agcatcccga gggagcgggc aaatttctgg gtcagcatcc cggtttcgtt gctggcaccg
    22801 gaggcacaca gcataccggt agaaagccaa cggtttaccg ttacgccctg ctcgttcttt
    22861 tcaataaagt tagcgtcacg gtcagctttc atcagcttcg caatacggga gaatgcttct
    22921 tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatattccgg gtagcgcaga
    22981 cggttttcgc tgttgacgta atccagcaaa ccgccccctt ttgggcacag cgcaccacgg
    23041 cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc gtttttcgcg
    23101 ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt gcaggtgtta
    23161 cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc ttgcttcggg
    23221 gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat tttaaaaaat
    23281 tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg cgcgaggaaa
    23341 ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg tatgccacaa
    23401 aataatccgc ctgcgcaaat taacctacct caatagcggt agaaaaacgc accactgcct
    23461 gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc aagagagaca
    23521 aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg atcgtcctgt
    23581 ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggtccg gtcggcggcg
    23641 cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga tttccgcgta
    23701 ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc agctatgaaa
    23761 tctgtctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt gaagtgggta
    23821 tggtgaacta tctgtggccc agcctgacaa ttctctttgc cattctgttt aatggtcaga
    23881 aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc tgctgggtgt
    23941 taggcggtga gaatgggtta cattatgatg aaatcatcaa taatatcacc accagcccat
    24001 tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc acagtaacga
    24061 ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg ggagcatgtc
    24121 tgtggattta ctattttctc acgccacaac cagaaatggt atttagcacg cccgtcatga
    24181 ttaaactcat ctctgcggca tttaccttag gatttgctta tgctgcatgg aatgtcggta
    24241 tattgcatgg caatgtcacc ataatggcgg taggctcgta ttttacgcct gtactttcct
    24301 cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg caaggcgcgc
    24361 taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt taaaataatt
    24421 gccgggtcta cggcccggca gttaatcaga taaattccga caatattaaa aaataaattt
    24481 caaataccag taattcac
//
LOCUS       DAZRVY010000055        23729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZRVY010000055 DAZRVY010000000
VERSION     DAZRVY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.055"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..800)
                     /locus_tag="ACHXOX_002260"
     CDS             complement(<1..800)
                     /locus_tag="ACHXOX_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR7005712.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
     gene            1451..1732
                     /gene="drpB"
                     /locus_tag="ACHXOX_002261"
     CDS             1451..1732
                     /gene="drpB"
                     /locus_tag="ACHXOX_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR7005713.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            1772..2428
                     /locus_tag="ACHXOX_002262"
     CDS             1772..2428
                     /locus_tag="ACHXOX_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR7005714.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2495..3913
                     /gene="dcm"
                     /locus_tag="ACHXOX_002263"
     CDS             2495..3913
                     /gene="dcm"
                     /locus_tag="ACHXOX_002263"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR7005715.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3894..4364
                     /gene="vsr"
                     /locus_tag="ACHXOX_002264"
     CDS             3894..4364
                     /gene="vsr"
                     /locus_tag="ACHXOX_002264"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR7005716.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="ACHXOX_002265"
                     /pseudo
     CDS             complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="ACHXOX_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5447..6364
                     /locus_tag="ACHXOX_002266"
     CDS             5447..6364
                     /locus_tag="ACHXOX_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR7005717.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6443..6625
                     /locus_tag="ACHXOX_002267"
     CDS             6443..6625
                     /locus_tag="ACHXOX_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR7005718.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6796..8490
                     /gene="dgcQ"
                     /locus_tag="ACHXOX_002268"
     CDS             6796..8490
                     /gene="dgcQ"
                     /locus_tag="ACHXOX_002268"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR7005719.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8487..9302)
                     /locus_tag="ACHXOX_002269"
     CDS             complement(8487..9302)
                     /locus_tag="ACHXOX_002269"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR7005720.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="ACHXOX_002270"
     CDS             complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="ACHXOX_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR7005721.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9990..10178
                     /gene="dsrB"
                     /locus_tag="ACHXOX_002271"
     CDS             9990..10178
                     /gene="dsrB"
                     /locus_tag="ACHXOX_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR7005722.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="ACHXOX_002272"
     CDS             complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="ACHXOX_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR7005723.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="ACHXOX_002273"
     CDS             complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="ACHXOX_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR7005724.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="ACHXOX_002274"
     CDS             complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="ACHXOX_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR7005725.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="ACHXOX_002275"
     CDS             complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="ACHXOX_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR7005726.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="ACHXOX_002276"
     CDS             complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="ACHXOX_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR7005727.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="ACHXOX_002277"
     CDS             complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="ACHXOX_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR7005728.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="ACHXOX_002278"
     CDS             complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="ACHXOX_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR7005729.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="ACHXOX_002279"
     CDS             complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="ACHXOX_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR7005730.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15296..16423)
                     /locus_tag="ACHXOX_002280"
     CDS             complement(15296..16423)
                     /locus_tag="ACHXOX_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR7005731.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="ACHXOX_002281"
     CDS             complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="ACHXOX_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR7005732.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="ACHXOX_002282"
     CDS             complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="ACHXOX_002282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR7005733.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="ACHXOX_002283"
     CDS             complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="ACHXOX_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR7005734.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="ACHXOX_002284"
     CDS             complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="ACHXOX_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR7005735.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="ACHXOX_002285"
     CDS             complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="ACHXOX_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR7005736.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21796..22110
                     /gene="fliE"
                     /locus_tag="ACHXOX_002286"
     CDS             21796..22110
                     /gene="fliE"
                     /locus_tag="ACHXOX_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR7005737.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(22135..22263)
                     /locus_tag="ACHXOX_002287"
                     /pseudo
     CDS             complement(22135..22263)
                     /locus_tag="ACHXOX_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22444..22776
                     /gene="emrE"
                     /locus_tag="ACHXOX_002288"
     CDS             22444..22776
                     /gene="emrE"
                     /locus_tag="ACHXOX_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR7005738.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22945..23496
                     /gene="ybcL"
                     /locus_tag="ACHXOX_002289"
     CDS             22945..23496
                     /gene="ybcL"
                     /locus_tag="ACHXOX_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR7005739.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23506..>23729
                     /locus_tag="ACHXOX_002290"
     CDS             23506..>23729
                     /locus_tag="ACHXOX_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR7005740.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAGL"
BASE COUNT         5759 a         5946 c         6139 g         5885 t
ORIGIN      
        1 attgcaagat aaacattgtt ggcgtcgtat tttgcaccaa atgtccatgc ttcagctgta
       61 tcaccaccag caaatttgct gtattcattg tagtgatacc cgccaagagc tacttgagaa
      121 ttggtacggt cagagtttga gtaagcggca cctaagctca aaccgaagtc aaagtcatat
      181 gaagtggaaa gaccaaaacc atcaccgttc tggaagcgaa cgtcttcgtg accattttta
      241 gtaccttctt gatcttctcc tgcattgctg ccttcgttgt taccttgata ctgcaatgca
      301 aagttcagac cttccaccag accgaagaaa tcactattgc ggtaagtagc taccccattt
      361 gcacggcctg ccatgaagtt atcagtccag gtataggagt cgccgccgaa tacaggaagc
      421 atatctgtcc aggcttcaac gtcgtaaatc acaccatagt tacgaccata gtcaaaggag
      481 cccatctcgc cgaacttcag acctgcaaaa gcaagtttga cataagtatt atcagaacct
      541 tcagtgtcgc tagcctttgt tttggtcagt gcttgaccat aaccagttaa ctgatctgta
      601 atttgggtct cacctttgaa gcctaactcg atgatagttt catcgccgtc atcgccaggg
      661 ttatcggaaa aagtatgacg agcatcaact tttccgtaca gatccagttt attgccattt
      721 ttattataaa tttcagccgc atttgctgcg ccagcaacta ataacgctgg gaccagcatt
      781 gccagaactt ttcttttcat tatgtattcc cttgtgatta taatcttcat gaatatatca
      841 ataagtgccg ttatccaaaa aaaaagcaca tttggatact attctatgaa gttcatttta
      901 ttttaaagat tgcgtgtaac aaatgtattt aaaagatttc aaaatgtttc taatctattt
      961 aatataattc agaattatga attctaaata taaacatcca tgaaaattat aaatgtatat
     1021 caatatgtta tgagttgaat ggaaagatag ggagtatcaa tgattgttgc gccaaacggc
     1081 aggcgtgatc acgcgcacat taatgaaggt ataaaaaaag ccggcatcat gccggcccaa
     1141 aaaatataaa caagacgata aaaggctaat aacggggtta tcatgacaca gaaatcaaaa
     1201 aaggtataat tgtattcagt gctaatatat tgtttgggtg aattaataaa actattacca
     1261 aggatataaa taaatcttta ttgctgtttt tccagtaatt attgccgttg cgcaaaattt
     1321 atctatttgt tcaaaaaatg attgagtctt gactggctca tccaatgtgg aaaaatgtga
     1381 cttttatcac ataatagtac taagtctgaa ttttccgggt tatctcaaaa tggaatacgg
     1441 ttcgacaaag atggaagaga gactctctcg cagccctggc ggcaaattgg ctttgtgggc
     1501 tttctataca tggtgtggct atttcgtctg ggcaatggcg cgttacatct gggtgatgag
     1561 ccggatccct gatgccccgg tgtcgggatt tgaaagcgat ttaggctcta ccgcgggaaa
     1621 gtggttaggg gcactggtcg gatttttatt tatggctctg gtcggggcat tgttaggcag
     1681 cattgcctgg tacacccgac ctcgtcccgc acgtagcaga cgctatgaat aaaaactgct
     1741 aaggtggcga ctctctggca acggagcgcg tatggactta caacactggc aggcacagtt
     1801 cgaaaactgg ttgaaaaacc atcatcagca ccaggacgcg gcacagaagc tggcggcaga
     1861 tgacgatgtc gatatgctgg tgattttaac cgcctgttat tttcacgata ttgtgagcct
     1921 ggcgaaaaat catccgcaaa ggcagcgttc ttcaatcctg gcggcagaag agacgcgtcg
     1981 cctgctccgt gaagagttcg agcaatttcc ggcggagaaa atcgaggccg tttgtcatgc
     2041 cattgccgct cacagtttca gcgcgcaaat cgccccctta acaacggaag ctaaaatcgt
     2101 ccaggatgcg gatcggctgg aagccctggg ggcgattggc ctggcgcgcg tgtttgccgt
     2161 ttcaggggca ttgggcgtgg cgctgtttga tggtgaagat ccgttcgcac agcatcgccc
     2221 gcttgatgat aaacgctacg cgctggatca tttccagact aagttgctga agctaccgca
     2281 aaccatgcaa accgcaagag gcaagcagct ggcgcagcac aacgcgcatt ttttagtcga
     2341 gtttatggcg aagctcagtg ccgaactggc gggggagaat gaaggtgtcg atcacaaggt
     2401 gatagatgcg ttttcacccg cgggataagc gcgtggccct aaatggctgt aattatgtta
     2461 acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat cagtaaccga
     2521 ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc aaatttatga
     2581 tgttaaaacg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga gcgcggcaat
     2641 tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga aagagttcgc
     2701 ccatctgcaa acgttgttac ccaaaccatc ggcacatcat ccgcattatg cgtttcgctt
     2761 tatcgatcta tttgccggaa ttggcggcat ccgtcgcggt tttgaatcga ttggcggaca
     2821 atgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag ccaaccatta
     2881 ttgcgatccg gcgacgcatc attttaatga agatatccgc gacatcaccc tcagccataa
     2941 agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc ctgaacacga
     3001 tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat cgaaaaagaa
     3061 ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt tttttgatgt
     3121 ggtacgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg tcaaaaacct
     3181 gaaaagtcac gaccagggta aaacgttccg catcatcatg cagacgctgg acgaactggg
     3241 ctatgacgtg gctgatgcag aagataacgg gccggacgat ccgaaaatca tcgatggtaa
     3301 acattttctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc gcgatcttaa
     3361 tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac agcgagtgac
     3421 gctggcgcag ctgttggacc cgatggtcga ggcgaaatat atcctgacgc cggtgctgtg
     3481 gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct tcggttatgg
     3541 aatggtttat ccgaacaatc cgcaaagcgt cacgcgtacg ctgtctgcgc gttattacaa
     3601 agatggcgcg gaaattttaa tcgatcgcgg ctgggatatg gccacgggtg agaaagattt
     3661 tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcggg aatgcgcgcg
     3721 cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt cggacactca
     3781 ggcctatcgc cagttcggta actcggtggt cgtgccggtc tttgccgcgg tggcaaaact
     3841 gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac aacatggccg
     3901 acgttcacga taaggccact cgcagcaaaa atatgcgcgc gattgccacg cgtgatacgg
     3961 cgattgagaa gcgcctcgcc agtctgttaa ccgggcaggg cctggcgttt cgcgttcagg
     4021 acgccagtct gcccggaagc ccggattttg tcgttgatga atatcgctgc gtgatattta
     4081 cccatggctg cttctggcat catcatcact gctatctgtt taaagtgcct gcgactcgaa
     4141 ccgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc gatatcagtc
     4201 gtttgcagga actcggctgg cgcgtactga ttgtctggga gtgtgcatta cgtgggcgcg
     4261 agaagctgac ggatgaagcg cttaccgagc gtctggaaga gtggatctgc ggcgaaggtg
     4321 ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcgtc ctgaataact
     4381 ggcgcaacta cgggttttgc cgggaagaga tattttccca acgtgaccag taccaccgcg
     4441 aagacaatta cgccgagcgc cagccattca atcttcgaca gcgtctctcc gcccagtccc
     4501 gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc gagagccgga
     4561 ctgacattac ggattaaata catataagcg ttgatggcga taatcgaacc aaacagcgcc
     4621 agatagccga ccgcaaggaa gcctgaaagg gaagggagcg ccgtcagttt ttcacctgca
     4681 atcatcgacg cgatcattaa caccacgcct gccgccagca tctcaatcgc acccgccatc
     4741 atccctacag gtaaggtaat gcgcgagcca taaaccgagc caaacgccca gctaatcgag
     4801 ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc gctattgagc
     4861 atgatgattc cggcaagccc aatggcgata cccacccatt ccagtttgcg cgttttaatg
     4921 ccaaacaggc ggctgaagca ccagggtaaa gaggggcacg gttgcaacca caacggcggc
     4981 gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac cgacagccag
     5041 caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga gcttgtgtcc
     5101 gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa cgcctgccat
     5161 cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg agccccaaat
     5221 gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac gcataatccc
     5281 tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg ctggcgagag
     5341 atataattgc acttgattgt aaaaaaaatg ttgacctgtg aagcaagtat cagagctgcc
     5401 gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc ttttggccgg
     5461 gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata tctccgtgat
     5521 gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat cgctcaatgc
     5581 gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg gcgtggcgaa
     5641 aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca gtgcgtttat
     5701 cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct ttgaaggagt
     5761 agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg cgcagagcca
     5821 gcagcgtctg gagaagctgg cggcgcagga tccgctgaag tttgaaaagg acaaaataaa
     5881 aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca cgctggggat
     5941 tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg cgctggtagt
     6001 gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc tggggtattg
     6061 gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat tgatcattgc
     6121 gccctggttg atgaaggcgt tatcgattgt cggcacgctg gcgatgttcc tcgtcggcgg
     6181 cggtattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt tcgccgggca
     6241 gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc ttggatttat
     6301 catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatgcgcg gtcaggcaca
     6361 ttaatactcg tacaggctat tatgcaaaat tcgccatctt caactaaggt gaacaagttt
     6421 cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta cggttaaaga
     6481 gggcggcccg cggatgattg tcactggata ctccagcggt atggttgagt gtcgttggta
     6541 tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc cgggggaggg
     6601 gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg atcacttccg
     6661 gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc accattataa
     6721 aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata aaaaagcagg
     6781 ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg gttgaaaaaa
     6841 ctcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt ggtattgctt
     6901 ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta tatctccagc
     6961 cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca gtataacgtc
     7021 gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc actggatttc
     7081 acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca cgcctggcaa
     7141 attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc attagtcagc
     7201 gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac cgctgcactg
     7261 gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga acaggcgatg
     7321 tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac gcgcaatgta
     7381 ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc gcaacgagaa
     7441 aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag caatactgaa
     7501 ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg cgtgctgggg
     7561 atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga taaaaacctc
     7621 gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc caatcctgac
     7681 catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca ggcgatggaa
     7741 catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga acgtctggac
     7801 catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg cggcgatttc
     7861 ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat gttactcatc
     7921 tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag ctcgttgcag
     7981 tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact gtttgaaaaa
     8041 gcccgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt cattcaggtc
     8101 gaccttgacc attttaaagc gattaatgac cgctttggtc atcaggcggg cgaccgtgtt
     8161 ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt tgccgggcgg
     8221 gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga ggctgcggaa
     8281 gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc taagagtacg
     8341 acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaaaccgg tgattatgat
     8401 tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca ggccgggcgt
     8461 aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc cagcccttca
     8521 cgccagcctt ccggtccttc acgctgcgtt cgccagacgc gggtcggatc ctcatcatgc
     8581 agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat tacctccagt
     8641 aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt gcctgataat
     8701 tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga ggcatccagg
     8761 acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag acgagcggta
     8821 aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc ctcatgcagc
     8881 tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt tgcatcgtcg
     8941 acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt taaaacctgg
     9001 ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat atcctgccat
     9061 tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtaaccc ttgtagcccc
     9121 aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag aatgacggga
     9181 acattcgctt cgcgtaaacg gctgagccag agggcagtcg gttgccagtc ataactatga
     9241 ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg aattgaaaac
     9301 atggtcgtgc agttctcccc ttaccaggat gattctcaaa acagcacaaa taatcaaaga
     9361 tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac gcacgtcaca
     9421 tttctattca taagtagcgt taattattca tatcgcgaat attttcttgt cagcgaaaaa
     9481 aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt tttaagtgct
     9541 tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt attgtgcatt
     9601 caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc atccgccaat
     9661 tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc gttctggctg
     9721 cgatgaactt cgctttcgct aatttcattg atcgccgcca gcagggcatc gacatcgacg
     9781 ctgacctcac gttttgcggt atcgctgtac tcttttgcgg ttttcatcat ctttcctcgc
     9841 aaccgttact ctgttgttgc cgccagtcgt taagtccgcc tggcatctct taagtataga
     9901 ccgtcgagaa aatcagcact cgcgcgcgcc tggagctgcc agaaaaacga aattgttcta
     9961 cactggcaca aagccacagg aggaaaacga tgaaggtgaa tgatcgggta acagtcaaaa
    10021 cggatggcgg tccgcgtcgt cctggcgtgg tactggcagt tgaggagttt agtgaaggca
    10081 caatgtacct ggtttcgctg gaagactacc cgctcggcat ctggttcttt aatgaagcag
    10141 ggcatcagga cggtatcttt gtggagaaag cagagtaatc ttattcagcc tgactggtgg
    10201 gaaaccacca gtcagaatgt gttagcgcat gttgacaaaa ataccattag tcacattatc
    10261 cgtcagtcgg acgacatggt agataacctg tttattatgt gttttgatct tacgtttaat
    10321 attaccttta tgcgatgaaa cggtcttggc tttgatattc atttggtcag agatttgaat
    10381 ggttccctga cctgccatcc acattcgcaa catactcgat tcggttcggc tcaatgataa
    10441 cgtcggcata tttaaaaaag aggttatcgt tgtctctttt ttcagaatat cgccaaggat
    10501 atcgtcgaga gattccggtt taatcgattt agaacttatc aataaatttt ttctgaccaa
    10561 tagatattca tcaaaatgaa cattggcaat tgccataaaa acgataaata acgtattggg
    10621 atgttgatta atgatgtgct tgatgtgctg actgttagaa gcatcatgga tgaaacagtc
    10681 ctcattaata aacaccactg aagggcgctg tgaatcacaa gctatggcaa ggtcatcaac
    10741 ggtttcaatg tcgttgattt ctcttttttt aacccctcta ctcaacagat acccggttaa
    10801 acctagtcgg gtgtaactac ataaatccat aataatcgtt gacatggcat accctcactc
    10861 aatgcgtaac gataattccc cttacctgaa tatttcatca tgactaaacg gaacaacatg
    10921 ggtcacctaa tgcgccactc tcgcgatttt tcaggcggac ttactatccc gtaaagtgtt
    10981 gtataatttg cctggaattg tcttaaagta aatgttgcga tatgtgagtg agcttaaaac
    11041 aaatatttcg ccgcaggagt atcctggaag atgttcgtag gtattatttt tcggataatc
    11101 cttaggataa catgataaac gttacggaat tatattaatg gcaattcact aataatatca
    11161 gccagcaaat taaacatctc actgaataaa tgttcgcaaa aaggtgcaat taacggcatt
    11221 aatgccgcca ttaaagagat accgacagtc agagttaatg gaaatccaat aacaaaaatg
    11281 gataattgcg gggccatacg attcagtaaa cctaatgcca gattcagggt cagcagcaga
    11341 gtaatgagcg gtaacgccag catcagcccg ttaaggaaaa tcaaactccc cgctttggtg
    11401 agtgccagaa acgcattgct gttcaacggt tcgccaccaa ttggcagggt gtgaaaggta
    11461 tcgaccagca gtgaaatcaa ccataaatga ccgttaaatg tcaggaacag cagtaacgcc
    11521 agcatatcca tgatacgcgc taaaacgggc atattgagat ggctggccgg atcgacaaac
    11581 gtcgcaaatg acagccccat ttgcagaccg ataatttcgc cagcggttcg cacagcggca
    11641 aaggcaaatt gcatggtaaa accaagcgca atgccgatca ggatctgctg cacggccagc
    11701 cacagagcaa agaacgaaaa aacaggaaca tcgttggcag gtaatgatgg ggcaatgacg
    11761 aacgtgatca tcattgccag ccccagtttt acccgtttcg gcacgctgcg ttcgctcaga
    11821 attggtgcgg tagaaatgag cgccagcacg cgcagtaacg gccagaagta caggcttaac
    11881 caggaaagcc attgatcgct tgtcacctgc atcattgtac ggcctacccg atgatatacg
    11941 gcaggttagt gaacaaggtg cggacgtaat ccagcaacag attgagcatc cacggtccgg
    12001 caataataat ggcaataaat acggcgatga tttttggaat aaacgacagc gtcatttcgt
    12061 tgatctgcgt ggcggcctgc aaaatactga tgataaggcc cgtgaccagc gccaccagca
    12121 acagcggggc agccagtgcc agcgcgactt tcatcgcttc agtccccatc atcatgaccg
    12181 attcaggtgt cattttgcct ctctagctgt aaaagctctg cgccagcgaa ccgaccagca
    12241 attgccagcc atccaccagt acaaacagca tcagtttaaa gggcagagca atggtggcgg
    12301 gggggaccat catcatcccc aatgccatca acacgctggc aatcaccagg tcgataatca
    12361 aaaaaggaat gaaaatcgtg aagcctatct gaaatgcggt tttcaattcg ctggtcacgt
    12421 aggccgggag caaaatgcgc atcggcacgg cttcaggtcc ctgcaacggg ccggtattcg
    12481 ctagtctggc aaacaaccct aaatctgcct cacgggtctg acgcagcata aactcacgta
    12541 gcggctgcgc ccctttttcc agcgcctcct gcattgatat tttctcttcg ctgaatggct
    12601 ggtacgcatc gacataaatt ttgtcgatca ccgatgacat aataaaaaag gtcaaaaata
    12661 gtgccagccc cagcaatacc tggttaggtg gcgcggaggg cgttcccagc gcgttacgca
    12721 ataaaccaaa aacaatgatg atgcgggtga aactggtcat catcagtaaa attgccggaa
    12781 taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg gagcgaccag ctttgtccac
    12841 cgccaggcag cggctggctg gtgatacccg gcagttgcgc gaaggcgagg ggcgtaacca
    12901 gccagagaag gacaggtgcg acagacaaca aacgacgcat caggatctcc cactacgctt
    12961 aagcaaattt ttcatgaccg actgaaaatc ggtctgcggt atctcttctg ttggtgctga
    13021 agggggaagt ttatgcagca gattgatttg ccctgcggta acgccgagca ccagccgtgc
    13081 atcttccaca tcgaccacca caacccgttc acgcgcgccc agcgaagcac tggcgctaat
    13141 tttcagaccg ttaacgccag tgcgtttcgg ggcaaatccc aaccgtttta ccagccaggc
    13201 agcagcgagg atcagggcaa taatggcgat gagtgcgccg ctgacctgca gcagtggcgc
    13261 agcagaaacc ggcgtggaag attgcacagt agcgtggtta ttcatcacta acggctcagg
    13321 cgacgcattc gctcagacgg agtaatgata tcggtgatcc gcacgccata tttatcggca
    13381 acgaccacca cttcgccctg ggcgattaaa taaccgttga tcagaatatc cagtggttcg
    13441 cccgccagac cgtccagcgc cacgacggac ccttgcgtca gacgcaacag ctctttgatg
    13501 gtcatccgcg tacggcccag ctcgacggtc agcttgaccg gaatatccat tatcaggtcg
    13561 atatcctgca acgttccgct gacatcacca ccgccaaatt gctggaacac cgcgtcggca
    13621 gcgcttttgc tgctggttga tttttgttcg ctcaacgctt cagcccacag atcgtccatt
    13681 gcgccgttgt tgtcatcggc cggattattc atgtcactca tttgggctgt tcctcgttca
    13741 gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa cgcatactga ccgttgagag
    13801 tgccgtactg actggtcagc accgggacgc cgtcaacatg ggcgatgatg ccatcgggtt
    13861 tttctatcgg caggacgtcg ccgggtttca gttttaaaat ctgcgacagg cgtagcgaga
    13921 tatcggcaaa gttggcgacc agctccagct gtgaatgctg cacctggcgc accaggttat
    13981 cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg cgggttaacc aacaattccc
    14041 gtagcggctc gatcatgctg aatggcagac agatattaaa ttcgccggtc aggttgccaa
    14101 tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg cgaggtggtg atattggtaa
    14161 atttcacctg catttccgaa cgcacgtact caacttccag cggattaatc gccttccagg
    14221 cgtcgctata gccttcaagc gccagcttta acatgcggtt gatgacgcgc tgttcggtat
    14281 gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc attgccgcca aacaggttat
    14341 ccacggcgat aaacaccaga ctcggtgaaa acaccaccag ccctgtgccg cgcagcggtt
    14401 tcagatggat aaggttcagg ttggtcggca ccggcaggtt gcgggcaaat tcatggtacg
    14461 gctgaatgcg gatggccccg acggttatat ccgggctacg acgcagcagg ttgaacagcc
    14521 ccatacgaaa atggcgggca aagcgctcat taatgatttc cagcgcctgc aaacgttcgc
    14581 gcacaacccg tcgttgggta ttcggatcgt acggacgaat gtcactttcg ccgctaacac
    14641 tggctgtcgg ttcgtctttg acttcgctgt caccattcaa cagcgcatca atttcagctt
    14701 gagaaagaat actatcgccc atgtcgttat cgcagaataa aagcggtata catcacgtcg
    14761 gtgacatcct gtttcggttg cccggcaaca agcggggtgg aaagcgtggt tttaatctcg
    14821 gcaatcaggt ttttcttgcc ttcttctgtc gccagtacgg cagcatcctg acgcgaaaac
    14881 agtaacagca aacggctacg gacttccggc aaatactcac tcagccgcga gcgggtagct
    14941 tcatctttga gtcgcagggt tataccgata taaagcacgc gatccgcatc gcccaaattg
    15001 accgtaaagg tatccagcgc atagaagacc ggtgagggca cgacgcgatg ctgcgctttg
    15061 tcttcagcgg caacctgatg cgaatgccag tagctgtaac ctgcgctggc acaggccgcg
    15121 agggtaatga ataccagaat cgggagccaa agcgatcgct tgcttttctt gcttatcgcg
    15181 taatcagtca tgtgttgcgg tcttcctgtg tcgctactgc ttatcggcta attatcctgc
    15241 gtcttgagcg caacaaagcg gggaaaagac gcggattacg gtgctacctc tgacgttagg
    15301 cgaaaatatc aacgccgctg ttgcctgtta cgcgcccttg taaagagacg ggaaccggaa
    15361 gcgtatcgtc ttcttccccc gccagaggtt catggtttac tgtgcgttgg ctttgctgtt
    15421 gttgggaagc ggcctgctgc tgaccactaa agctttcgcc actgatgttg ctttgcccta
    15481 actgaatgcc actctcggcc agctgcgtac gcagtaccgg cagcgctgct tccagggcgg
    15541 cgcgtacgtg ctgatgcggc gaaaccatct ggatttgcgc ctggttatca tccactttga
    15601 gggagatttg cacttcacct aaatcctgcg ggtgcagacg caactctgca ctttgttgtc
    15661 cctggcgggt gaacagcgaa atatgctggc ttaatgattg ttgccattcg tgagaaccca
    15721 gcggtgcact caagacaggc gcggcgacgg tgagcagtgg ctgtgtctgg tgtggagtga
    15781 ttagcgggct ggcggcagcg gtcaccggtg aaggtgtgct gatgacttcc gctttactct
    15841 gggcttctgc taccagcggt gttaatggct gagctggcgt gccgggggca tcatcaggct
    15901 gtgctgttgt gagttgcgca gaagtcagtt ttgtgaagag cgttggtttc tctgccggta
    15961 acacggttga cggcgcatca gtcactttgg gcgtattgtc aaaacccggc aacatcgcaa
    16021 aaagggcgct caggcttgcg gtgaggtctt cattcagatc atccgctttt tcgtcttttg
    16081 tcgtattttt gtcagccacc gcagccaacg tcatcgtatg agcggtggtt aacggtgtcg
    16141 atgtggattg ttcgacgttg atgacaggca gtgtttcatc cacagggatc agtaaatccg
    16201 cttgttgcgc gtcggaaaca atatcgctga tcagcagctc gccttttgtc gtgggcttat
    16261 ctgttgccac cagcacctgg ggggccgctt tgtcggtagt tgtctcgctt gctaatgctt
    16321 cgctcaacaa cgcgagaaaa tcttgtgcag catcgctggc tttgccgcca ggcaatgtgg
    16381 tggtgtcaac gtcggcggta atcaagggcg ctaagcgaat cattcaggtt tcctcatggc
    16441 ggcgcgctgg gcgaactcat ccatcttttt ctgatcgagg cggttttctg caagcagtgc
    16501 cgccgtggat tgccgttcct gcagtgtctg ccaggcctgc aaacgttgtt tcttttctcg
    16561 ccagctgttc agggcaatgt caactttctg cgtccactga ttaagttgct ggcgatgctg
    16621 agtaatggct ttttccagcg tctggataaa ctgctgatag ttgatccagc ggttgctggt
    16681 catcccggca ctcatatcgc tgttgaggtt attgcgatat tcattctgat aatcgatcag
    16741 cattttgagc tgttcttccg cctgctgaca cccgcgacgc atttcaccca gcaggcgcgc
    16801 ggcatcctct acctcttttt ctgccagatc tttcagggtc gccagcgcac catgttctgc
    16861 catctaccgt tatctcctgg gttatgacac tgtcgggaaa atacgctcca gcccctggag
    16921 agacgcttcc cagtccgcac gttcaaaaat gccttgttgt aaatagccct ccagctgcgg
    16981 ccacagggcg atggctttat cgagcatcgg atcgctgcct ttggcatacg cgccgacgct
    17041 aaccagatcg cggttacgct gaaaactcga caacagctgt ttgaaggtgc gcactcgcgc
    17101 gtaatgttgc tcactgatca acgccgtcat tgcgcggctg atcgacgctt caatatcgat
    17161 agccggatag tgcccggctt ccgccagtcg gcgagacagc acaatgtgac cgtcgaggat
    17221 cgcccgtgcg gagtctgcaa tggggtcctg ctggtcatcg ccttcagtaa gcacggtata
    17281 aaacgcggta atcgagccgc cgccgctaat gccatttccg gcacgctcga ccagtgccgg
    17341 taatttggca aacaccgacg gtggataacc tttggtggcg ggtggttcgc caatcgccag
    17401 cgcaatttca cgctgggcca tcgcgtagcg ggtgagggag tccataatca gcaacacatg
    17461 ctgaccacga tcgcgaaaat cttcggcaat gcgcgtggca taggcggcac cttgcattcg
    17521 cagaagcgga gagacatccg ccggagcggc aatcaccacg gagcgtgcac gcccttcagc
    17581 accgaggata ttctcaataa aatcttttac ttcgcgccca cgttcaccaa tcaaacccac
    17641 gacaatgaca tcggcgcggg tgtaacgtgc catcatcccc agcagcacac ttttaccaac
    17701 gccggacccg gcaaacagcc ccatacgctg cccacgccca acggtaagca gggcattgat
    17761 tgggcgcacg ccggtgtcca gcacatgttc aatcggtgta cgttgcaacg ggttaaatgg
    17821 cggggtaatc agcgcaccgg tttccgtcgt atcgggggag ggcaggccat cgagcggctt
    17881 accgctgccg tccagaacgc gacctaataa cgccggaccg agcggcaact gcttgccgct
    17941 ttgcagtcct tcagccgaaa tgtttttggc ataaacccgc gcgccgggca ggacaccttc
    18001 cacttcctcc agcggcatta aaaacagccg ttgaccgtta aagccaacga cttcgctttc
    18061 tacttcgtgc gtttcgctgc cgttctggcg ttcaatgaca caggttgcgc cgagcggcaa
    18121 ttgtaatccg gtggcttcca gcaccagccc ggtagcgcgg gttaatcgcc catagcgacg
    18181 taccgcaggc aactgcgcca ttttggcttc aaagttatcc agcgtggtta gccagcgagt
    18241 caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc ttgccagcga
    18301 gtggcgacac tggcgtcgag atcgccttca tcggcggaga ctttacagcc gccaggatgg
    18361 agtgtgggat cgccccgcaa gcgccagcca tgcaaactta aggttgcgcc gagcatatca
    18421 tccacacgtt gcagatcatc cgggtgcacg cgcagctgtg gtttaccgct gaataacggt
    18481 tcttgctgca acaactgttg gatctgtttg atcagtgccg agttatccac tgttggcgtc
    18541 tgaccgatca cctgacgtgc cgcctccagc gccatctgca tcaggcgcga agcaatcaca
    18601 ctatcaagcg catcaagggt agtttgaaat tcgctgacca gttgctgcat ccgggcatga
    18661 attggcgctt gttgagactt cgcctctgcc agaccttgct ccagcccctg ggccagtcct
    18721 tcctgatagc cctgctcatg accttgctgg cgaccttcgg caatccccgc ctgataacct
    18781 tgctcatggg cctgcatttg cagttgcgcc agttgctgct caaggctagg ttcggcctct
    18841 tcaatgatgg tttcttccgg ctcgaccatg ggcacaaact ctgcctgtgg tggcgcgaga
    18901 tcgtccggcg tccaggtttt ccacggcaga ttatcagaca taggtatcct cgccgctgcc
    18961 aattaccatc tcgccagttt cggcaaggcg gcgcacaatc agcagaatcg ctttctgttc
    19021 gttttccacc tgcgacagac gcaccggacc acggttggcg agatcgtcgc gcagaatatc
    19081 ggcggcacgc tgcgacatat tgcgcaggaa tttctcgcgc agtggctgct cggccccttt
    19141 cagcgcgatc aacagcgatt cggaatccac ttcctgcaac agacgctgaa tgctgcgatc
    19201 gtcgacatcc accagatttt cgaacaggaa catctcgtcg ataatttttt gcgccagctc
    19261 gccgtcgaat tcgcgcacgg cggtaataac ggcttcttcc tgctgagttt tcatcaggtt
    19321 gataatttcg gctgccgttc tcacgccgcc cattttgctg cgcttgagat tctgaccgtc
    19381 gagcaagcca ttcagtactt cggtcagttc cgccagcgcg gctggctgca cgccgccaaa
    19441 ggtggcgata cgcaacatca cgtcgtggcg cagacgttca tcgaacaacg ccagaatatc
    19501 ggcggcctgc gcgcgcttca gatgcaccag aatggtggcg ataatttgcg gatgctcatc
    19561 gcgaatcaga tcggcggcac tctgcggctc cataaagttg agcgtttcaa taccgctggc
    19621 ggtatcgcga gtttcgagaa tatcttccag caggctggcg gcacgttcct cacccagagc
    19681 tttgaccaat accgaacgca gataatcgtt ggcgttgata ttcagtgcgg caaactgttc
    19741 agcttcttgc tcaaactccg ccagcacata ggttagctgc ttgttggaga tctgcgtgac
    19801 gttcgccatt gcagcgctca gggtttgcac ttcgcgctgg gagaggtgct tgaacacctc
    19861 tgccgcccgg tcttcgccaa tggtcatcag caggatgacg cttttatcgg tgcctgtcag
    19921 gttactcatg atcgttattt atccactggc gaatgaccag cgccaccacg cgcggatcgt
    19981 tatcagacat ttcacggata cgctggctca tgacttctgc ccccagacgt tggttagcgc
    20041 gtcgttgttg gagttgttcg tctttgctca ggcggacttc caccgcatct tccacttcct
    20101 cacgggcctg cgcctgttgc tgtacagctt tcatcgcctc ggcgcgacgt gttaactgcg
    20161 ggcgtaccgc tttccgccac agcagccacg ccaccagcag taccagcaac cagcgaccgg
    20221 cagccagcaa ctgatcgata aacgcttgct gttgccagaa cggcagttct ccgccgcttt
    20281 cgtcactgct attgaacggc gagttaacga cattgagcga gtcaccgcgt ttttcagaaa
    20341 agcccatcgc ctcgcgggtc agatcttcaa tttgcttcat ctgttcgttg ctgagaggca
    20401 atggtttacc gtctggcaag gttttgtaat tcaccactac cgcgactgac agacgttgca
    20461 catcgcccac gttcattttg gtatgacgaa tggtgcgatc gacttcgtag ttactggttt
    20521 cattccgctg tgtgctacgc ggcccgctat tgctggtggt gctcgcctgc tgctggcggt
    20581 tattttgatt tgccggaggc gtgctgattg gcgcgttatt cgcaggtgcc ggttgattcg
    20641 acaacgcccc cggtacgccg cccggataac cggaaccgct ttgctcgctc tcattaagct
    20701 ggcgtgaacg aagcgccgca tgagattcat caccgttagg gcgatactgt tcttccgttt
    20761 gttctttact ggcgaagtcc agttgcgccg tgacctgggc gtgaatatta ccgttaccaa
    20821 caataggcga caggattgct tcaatacgcc gctgaatacg gccttcgaca tcgctggcat
    20881 atttcaactg agcgtcatta agatcgcgcc cgctggtatt ggactgggtt aacagatgtc
    20941 cgccctgatc caccagcgtg acgtttcccg gcggcagacc agcaacggcg ctggaaacca
    21001 ggtgcacaat ggcgctaatt tgcccttcat cgagtgcgcg gccgggtaac agatttaccg
    21061 tcaccgatgc agaaggggat ttttgttcac ggacgaataa agacggtttc ggcattgcca
    21121 gatgtacgcg cgcccctttt accgggccga tagtttcgat ggtacgagaa agctcgcctt
    21181 ccagcgcccg ctgataattc acctgttcgc tgaactggct gataccaaac ttttcctgat
    21241 caagcagttc gaaaccgacc gcgccgcctt tgggcaaacc ttgttgtgcc aggcgcagac
    21301 gcagttcgtg aactttatct gccggaactt caatagcgcc gctggcttcg ctgaagcagt
    21361 aaggaatatt catttgcgtc agttggctga caattgcgcc accatcctga tcggaaagat
    21421 tgctgaataa tgtgcggtag tcgggggctt tcgcccacag gatcagtgcg accatgaccg
    21481 ccactgccgc ggaaccggca acaatcaatg gaattttcgg attcgcacgc aggcgattaa
    21541 gccactcaag agattttgtc tgggctgcag tcgcattcat cgcgcacctc gtggctgatt
    21601 atggacggcg ttattggcaa agtggaacaa gactcactcc cgggtcagca aactcgaaaa
    21661 attgacgctg ccattatttg tgctttcgtt tttttctatc cgccaataaa cccgtttttt
    21721 ttgttgctat ttagcgcctt tgtcttattg acttactggt aagctttgct accagaaatt
    21781 atccgggaga tgaaaatgtc agcgatacag gggattgaag gggttatcag ccagttacag
    21841 gctacggcga tgagtgcgcg tgcgcaggaa tcactgccac aaccgaccat tagttttgcc
    21901 gggcagctgc acgccgcgct cgatcgcata agcgatacac aaacagccgc ccgcacgcag
    21961 gcagaaaaat tcactctcgg tgaacccggc gtggcgttaa acgatgtgat gaccgatatg
    22021 caaaaagcct cagtttctat gcaaatgggg attcaggtgc gtaataagct ggtggcggct
    22081 tatcaggaag tgatgtccat gcaggtgtag gttttgtaac ctgttgttaa ttacatccga
    22141 tctcaccgcg tggggcatgg atggggcaaa ctcactcaat ttctggttta aaatggatac
    22201 ctgatcctga aagttttctg ccatccacga tccgtaaacg cgataacatg ttttgtcttc
    22261 gctttgctca taggaagagt gaactctttt gttagcctgg ggttccttct gatcattatt
    22321 gttcctgcct ttatctcttg ccacgccaga gccccctgga tacacataca ccaggatgaa
    22381 aacggtgagt tgtgttcaaa ctgtagtaca attctctcca gtatgaacag gaaagaatat
    22441 gctatgaacc cttatattta tcttggtggc gcaatacttg cagaggtcat tggtacaacc
    22501 ttaatgaagt tttcagaggg gtttacacgg ttatggccct ctgttggcac aattatttgc
    22561 tattgtgcat cattttggtt attagctcag acactggctt atatacctac agggattgct
    22621 tatgctatct ggtcaggagt cggtatcgtt ctgattagtt tgctttcctg gggatttttt
    22681 ggccaacgac tggacctgcc agccatcata ggcatgatgt tgatttgtgc cggtgtgtta
    22741 gtcattaact tgttgtcacg aagcgcaccg cattaaaaaa taatcatcct tgagataaca
    22801 ggaatctgat ttgtgcatat tatggctaat gttcggggca actttataat tatgattgca
    22861 tttgcgcgat cattgcgcct ttttgcataa tgagatcaat gcacatcaaa taaaataacg
    22921 ataggtcaca acaaagaggt ttttatgaaa aaacttatcg tttcaagtgt attggcattc
    22981 ataacatttt ctgcgcaggc cgcagcattt caggtcacta gtaatgaaat aaaaacagga
    23041 gagcaactta caacgtctca tgtattttct ggagttgggt gtgagggggg taatacatcg
    23101 ccctcattaa cctggtctgg tgctccagaa ggcaccaaaa gctttgccgt aactgtgtat
    23161 gatccagatg cgcctacagg cagcggatgg tggcactgga ctgttgctaa tattccagca
    23221 acggtaacat atttgcccgc tgatgcagga agacgtgatg gtacaaaact tccgactggt
    23281 gctgtccaag gccgaaatga ttttggctat gcgggatttg gtggagcatg tcctcccaaa
    23341 ggagataaac cccatcatta ccagtttaaa atatgggctc taaaaactga taagattcct
    23401 gtggattcta attccagcgg agcgttagtt ggttatatgc ttaatgccaa taaaattgca
    23461 accgctgaga taatacccgt ttatgaggta aagtaggata agagtatgct ggcaaaagat
    23521 aggactaacc taaagattga agaaatacgg atgcataaac atcatgagat tcacagggtt
    23581 aagcctctta tgccagcttc atgtcgtatc cgtcagggaa aaaaagttat caattgggag
    23641 acgcatagtc taactgttga taataatcag ataatattat ttccctgtgg atatgaatct
    23701 tatattgcga attacccaga agcaggtct
//
LOCUS       DAZRVY010000056        23539 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZRVY010000056 DAZRVY010000000
VERSION     DAZRVY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.056"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHXOX_002291"
     CDS             <1..2064
                     /locus_tag="ACHXOX_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR7005741.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHXOX_002292"
     CDS             2080..3099
                     /locus_tag="ACHXOX_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR7005742.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHXOX_002293"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHXOX_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR7005743.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHXOX_002294"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHXOX_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR7005744.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHXOX_002295"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHXOX_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR7005745.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHXOX_002296"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHXOX_002296"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR7005746.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHXOX_002297"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHXOX_002297"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR7005747.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHXOX_002298"
     CDS             6828..7646
                     /locus_tag="ACHXOX_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR7005748.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHXOX_002299"
     CDS             7698..8444
                     /locus_tag="ACHXOX_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR7005749.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHXOX_002300"
     CDS             complement(8418..9383)
                     /locus_tag="ACHXOX_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR7005750.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHXOX_002301"
     CDS             complement(9380..10384)
                     /locus_tag="ACHXOX_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR7005751.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHXOX_002302"
     CDS             complement(10381..11658)
                     /locus_tag="ACHXOX_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR7005752.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHXOX_002303"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHXOX_002303"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR7005753.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="ACHXOX_002304"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="ACHXOX_002304"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR7005754.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHXOX_002305"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHXOX_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="ACHXOX_002306"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="ACHXOX_002306"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR7005755.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="ACHXOX_002307"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="ACHXOX_002307"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR7005756.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="ACHXOX_002308"
     CDS             16207..17562
                     /locus_tag="ACHXOX_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR7005757.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="ACHXOX_002309"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="ACHXOX_002309"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR7005758.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="ACHXOX_002310"
     CDS             18756..19529
                     /locus_tag="ACHXOX_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR7005759.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHXOX_002311"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHXOX_002311"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR7005760.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /gene="yegR"
                     /locus_tag="ACHXOX_002312"
     CDS             20916..21233
                     /gene="yegR"
                     /locus_tag="ACHXOX_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR7005761.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHXOX_002313"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHXOX_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR7005762.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="ACHXOX_002314"
     CDS             complement(23071..23403)
                     /locus_tag="ACHXOX_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR7005763.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6069 a         5513 c         6029 g         5928 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccataa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcagttggta
    23521 atgactccaa cttattgat
//
LOCUS       DAZRVY010000057        23493 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZRVY010000057 DAZRVY010000000
VERSION     DAZRVY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.057"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXOX_002315"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXOX_002315"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR7005764.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHXOX_002316"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHXOX_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR7005765.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXOX_002317"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXOX_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR7005766.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXOX_002318"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXOX_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR7005767.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXOX_002319"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXOX_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR7005768.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHXOX_002320"
     CDS             5280..5738
                     /locus_tag="ACHXOX_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR7005769.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHXOX_002321"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHXOX_002321"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR7005770.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPALIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHXOX_002322"
     tRNA            6790..6874
                     /locus_tag="ACHXOX_002322"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHXOX_002323"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHXOX_002323"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXOX_002324"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXOX_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR7005771.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXOX_002325"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXOX_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR7005772.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXOX_002326"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXOX_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR7005773.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHXOX_002327"
     CDS             complement(10156..13899)
                     /locus_tag="ACHXOX_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR7005774.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHXOX_002328"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHXOX_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR7005775.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHXOX_002329"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHXOX_002329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR7005776.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHXOX_002330"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHXOX_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR7005777.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHXOX_002331"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHXOX_002331"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR7005778.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHXOX_002332"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHXOX_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR7005779.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHXOX_002333"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHXOX_002333"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR7005780.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHXOX_002334"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHXOX_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR7005781.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHXOX_002335"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHXOX_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR7005782.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHXOX_002336"
     CDS             23294..23401
                     /locus_tag="ACHXOX_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7005783.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5884 a         6145 c         5666 g         5798 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaaag gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgcttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctc
//
LOCUS       DAZRVY010000058        22862 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZRVY010000058 DAZRVY010000000
VERSION     DAZRVY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.058"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            134..763
                     /locus_tag="ACHXOX_002337"
     CDS             134..763
                     /locus_tag="ACHXOX_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR7005784.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            790..2451
                     /locus_tag="ACHXOX_002338"
     CDS             790..2451
                     /locus_tag="ACHXOX_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR7005785.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2690..2749
                     /locus_tag="ACHXOX_002339"
     CDS             2690..2749
                     /locus_tag="ACHXOX_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7005786.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3066..3125
                     /gene="ibsE"
                     /locus_tag="ACHXOX_002340"
     CDS             3066..3125
                     /gene="ibsE"
                     /locus_tag="ACHXOX_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR7005787.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3245..4678)
                     /gene="hldE"
                     /locus_tag="ACHXOX_002341"
     CDS             complement(3245..4678)
                     /gene="hldE"
                     /locus_tag="ACHXOX_002341"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR7005788.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4726..7566)
                     /gene="glnE"
                     /locus_tag="ACHXOX_002342"
     CDS             complement(4726..7566)
                     /gene="glnE"
                     /locus_tag="ACHXOX_002342"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR7005789.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7589..8890)
                     /gene="ygiF"
                     /locus_tag="ACHXOX_002343"
     CDS             complement(7589..8890)
                     /gene="ygiF"
                     /locus_tag="ACHXOX_002343"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR7005790.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9132..9752
                     /locus_tag="ACHXOX_002344"
     CDS             9132..9752
                     /locus_tag="ACHXOX_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR7005791.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9816..11054
                     /gene="cca"
                     /locus_tag="ACHXOX_002345"
     CDS             9816..11054
                     /gene="cca"
                     /locus_tag="ACHXOX_002345"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR7005792.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11217..12038)
                     /gene="bacA"
                     /locus_tag="ACHXOX_002346"
     CDS             complement(11217..12038)
                     /gene="bacA"
                     /locus_tag="ACHXOX_002346"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR7005793.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12129..12497)
                     /gene="folB"
                     /locus_tag="ACHXOX_002347"
     CDS             complement(12129..12497)
                     /gene="folB"
                     /locus_tag="ACHXOX_002347"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR7005794.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12602..13219
                     /gene="plsY"
                     /locus_tag="ACHXOX_002348"
     CDS             12602..13219
                     /gene="plsY"
                     /locus_tag="ACHXOX_002348"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR7005795.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13232..14164)
                     /gene="ttdR"
                     /locus_tag="ACHXOX_002349"
                     /gene_synonym="dan"
     CDS             complement(13232..14164)
                     /gene="ttdR"
                     /locus_tag="ACHXOX_002349"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR7005796.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14371..15282
                     /gene="ttdA"
                     /locus_tag="ACHXOX_002350"
     CDS             14371..15282
                     /gene="ttdA"
                     /locus_tag="ACHXOX_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR7005797.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15279..15884
                     /gene="ttdB"
                     /locus_tag="ACHXOX_002351"
     CDS             15279..15884
                     /gene="ttdB"
                     /locus_tag="ACHXOX_002351"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR7005798.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15933..17396
                     /gene="ttdT"
                     /locus_tag="ACHXOX_002352"
     CDS             15933..17396
                     /gene="ttdT"
                     /locus_tag="ACHXOX_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR7005799.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17439..18452)
                     /gene="tsaD"
                     /locus_tag="ACHXOX_002353"
     CDS             complement(17439..18452)
                     /gene="tsaD"
                     /locus_tag="ACHXOX_002353"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR7005800.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18689..18904
                     /gene="rpsU"
                     /locus_tag="ACHXOX_002354"
     CDS             18689..18904
                     /gene="rpsU"
                     /locus_tag="ACHXOX_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR7005801.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19015..20760
                     /gene="dnaG"
                     /locus_tag="ACHXOX_002355"
     CDS             19015..20760
                     /gene="dnaG"
                     /locus_tag="ACHXOX_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR7005802.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20955..22796
                     /gene="rpoD"
                     /locus_tag="ACHXOX_002356"
     CDS             20955..22796
                     /gene="rpoD"
                     /locus_tag="ACHXOX_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR7005803.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5545 a         6236 c         5842 g         5239 t
ORIGIN      
        1 ataaaaaact atattaaaaa actttctctt ccagaacttc tctaacatgt gttattatta
       61 acaaagataa atgtaagcaa ggaaactatc ccaaagtaaa tattgcgtca ctaaatggac
      121 attggagtga catatgattt tattcgccga ctataatacc ccttatcttt tcgctatttc
      181 ctttgtactg ttgatcggcc ttttagaaat tttagcgctt atctgcggtc acatgctctc
      241 cggtgctctt gatgcacatc ttgaccatta taattccata accacaggtc atattagcca
      301 ggcacttcat tatctcaata tcggaagatt gccagctctc gtcgttctct gcttgctggc
      361 tggttttttt ggtcttattg gcatcctgtt gcagcatgcc tgcatcatgg tctggcaatc
      421 accactgtcg aacctgttcg tggttcctgt cagtttactg tttacgatta ttgcggtgca
      481 ttataccggt aaaatagttg cgccctggat tccacgcgac catagttcag ctattacaga
      541 agaagaatat attggtagca tggcattaat taccggtcat caagccactt caggtaatcc
      601 ctgcgaagga aaactcaccg atcaatttgg ccaaatacat tatttactac tggagcctga
      661 agaaggaaaa attttcacta aaggagtcaa agtgttaatt atttgccgac tttctgcaac
      721 gcgatatttg gcggaaaaca atccctggcc acaaatattg taatttatta atcaaaagga
      781 aatttaataa tggatgatat tgttaattct gtgccctcct ggatgtttac cgcgattatt
      841 gccgtatgca ttctgtttat tattggaatt attttcgcca ggctctatcg tcgcgcttcg
      901 gcagagcaag cttttgttcg tactggttta ggtgggcaaa aagtggtgat gagcggtggc
      961 gcaatcgtga tgccgatctt tcatgaaata atccccatca atatgaatac tctgaagctg
     1021 gaagtcagcc gctcaaccat tgatagcctg attacgaaag atcgtatgcg tgtcgatgta
     1081 gtagtcgctt tctttgtacg ggtaaaaccg tctgtagaag gcatcgccac tgcggcgcag
     1141 acgttggggc aacgcacgct atcaccagaa gacttacgta tgttggttga agataaattt
     1201 gtcgatgccc tccgtgcaac agctgcacaa atgaccatgc atgagttaca ggatacccgc
     1261 gagaacttcg tgcagggagt acaaaatact gtcgcagaag acctatcgaa aaacggtctt
     1321 gagctggaaa gcgtttcact taccaacttt aaccagacct cgaaagaaca tttcaatccg
     1381 aacaatgcct ttgacgccga aggtttaacc aaactgactc aggagacgga gcgccgtcgt
     1441 cgcgaacgta acgaagttga acaggatgta gaagttgcgg tgcgtgagaa aaaccgtgat
     1501 gcactttcgc gcaagttgga gattgaacaa caagaagcgt ttatgacgct tgagcaggag
     1561 cagcaggtta aaacccgtac tgccgaacag aatgcacgta ttgcggcttt tgaagctgaa
     1621 cgtcgtcgtg aagcagagca gacgcgaatt ctggctgaac gacaaattca ggaaacggag
     1681 atcgagcgcg agcaggccgt ccgctcaaga aaggttgagg ctgaacgtga agttcgtatt
     1741 aaagagatcg aacagcagca ggtcaccgaa atcgccaacc agacgaaatc gatcgctatt
     1801 gccgccaaat cggaacaaca gtcccaggca gaagcgcgtg ctaaccttgc actcgcagaa
     1861 gcagtaagtg cgcaacaaaa cgtagaaacc actcgccaga ccgcagaagc cgatcgtgct
     1921 aaacaagttg ccctaatcgc tgccgcgcag gatgcagaaa ccaaagcggt tgaactgacc
     1981 gtgcgggcga aagcagaaaa agaagccgca gagatgcagg cggcggctat cgttgagtta
     2041 gccgaagcta cacgtaaaaa gggtctggcg gaagcagaag cacaacgtgc gctgaacgat
     2101 gctatcaacg tactttctga tgaacaaacc agccttaaat tcaaactggc cttgttgcag
     2161 gcgctacctg cggtaataga gaaatccgtt gagccgatga agtccattga cggtatcaag
     2221 attattcagg tcgatggtct gaatcgtggc agcgctgcgg gtgatgcaaa cacgggtaat
     2281 gtggggggcg gaaacctagc ggaacaagca ttatcagccg ctctctctta ccgcacacag
     2341 gcaccgctga ttgactcctt gctcaatgaa attggcgttt caggcggctc actggcggca
     2401 ttgacttcac ccttaacctc aacaactccc gtcgccgaaa acgtagaata aattttcagc
     2461 tatgaacgtc cctggcacaa aaagccgggg attttttata tctgcgttcc gctaaaaact
     2521 gcaaatgatc aggccgttgc aacgttttgc gtgaccgctc ggggaaggca aaactgcctc
     2581 tgggaaagcg ttgcgcgagg tccggcgctc atcaacaatc ggggagtcag caaagggctg
     2641 aaacgggaaa gcccctcccg aggaaggggc cttacagaag gaaagggtta tgatgaagct
     2701 cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac agccaatcag
     2761 aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa aaaatcagcg
     2821 tcaagaaggt ttcattaaca catatccctg gcccaaaaag ccggggattt tttatatctg
     2881 cgttccgcta aaaggtgcaa atgctcagga cgttgcagcg ttttgcgtga tcgctcgggg
     2941 aaggcaaaac tgcttcaggg aaagcgttgc gcggggtccg gcgctcatca acaatcgggg
     3001 agtcagcaag gggctgaaac gggaaagccc ctcccgagga aggggcctta tagaaggaaa
     3061 gggttatgat gaagttcgtc atcatactgg ttgtgttgtt actgttaagt ttcccgactt
     3121 actaacaact gatcagaggg ggaagaaatc ctcccttacc cttgttcctt tactttaggt
     3181 tgaaaaaaca acagcgtcaa taggcctgcc tgctacaaag cgagatctgt gaaccgcttt
     3241 ccggttagcc ttttttatcc tgttggatct tcttgatgat gttagtggtc gagcaaccgt
     3301 cttcgaagtt gagcaccagc acttcgccgc cgttggccca gacttcttta ctcccggcga
     3361 tctcttctgg tttatagtcg ccgcccttca ccagtaaatc tggcaggatc ccggcgatca
     3421 agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc gaccgcttcc agtgcgccca
     3481 gcacaatcat acgctgttcg agcgggttaa cggggcggga atcccctttc agccgtttgg
     3541 tggaggcatc gctgttcaca gcgacaatca gacggtcacc cagtttgcga gcatttgcca
     3601 gataggagac gtgcccggcg tgcaggatgt caaagacacc gttggtcatt accacctttt
     3661 cgccgcgttt acgcgctgcc gctacggcca gcttcagttc ctcttcggtc atcacgccaa
     3721 agccggtatc tgcgcgccca cgcactgcat tttccagctc gatcggcgaa accgtggagg
     3781 ttcccagttt gccgaccacc acgccagccg ccgcattggc aaagaagcag gcttcttcca
     3841 gcgaattacc cgctgccagc gttgccgcca ggacgccaat caccgtgtcg cccgcaccgg
     3901 taacgtcata cacttcctgc gcctgggttg gcatatgcag cggcgcttta cccggttgca
     3961 gcagcgacat accctgttcg gaacgggtca ctaacagagc cgagagttcg taatcggcaa
     4021 tcagtttcat gccgcgctca acaatctctt cttcggtctt acatttaccg acaacagctt
     4081 caaattccga gagattcggc gttaacagcg tagcgccgcg gtagcgctca aaatcggtac
     4141 cttttggatc aatcagcacc ggaacgcccg ctttacgcgc cagttggatc atctgctgta
     4201 cgcttgccag cgcacctttg gcgtagtcag aaagcaccag cgcgccaatc gaactcagcg
     4261 cctgattaat ccgctcgtgc agcggctgcg gatcaacgcc ttcgaaacct tcttcgaaat
     4321 ccagacggat cagctgttgg ttgcgggaaa gtacccgtaa tttggtgatg gtcggatgcg
     4381 tcggtacaga aacgaagtcg catttgacgt tgacgtcggc cagagattta ctcagcgcgc
     4441 gcgctgcatc gtcaatgccc gtcaacccga ccaggcgtgc attagcaccg agagaagcga
     4501 tattcatcgc cacgttagcc gcgccgcccg gacgttcttc gatggtattc actttaacca
     4561 cgggcaccgg cgcttccggc gagatacggc tggagggacc gtaccagtaa cgatccagca
     4621 ttacatcacc aaccaccatc actcctgcac gttcaaactc tggcagcgtt actttcattc
     4681 ctgtctcctg agagattcaa aatttgcgcg cgataatacc atacttcatt cttccaccag
     4741 ccacttctgc cagcttgccc gcaccagatc acgctctgcg gtgaagcaat cccccgacac
     4801 atggcccggc aattcctgta atgccagatg atgaagttca tcgcgcagcg tcgtgtaagc
     4861 acgggtcagc gccatcgctt cctgctcttc cataatgtcg ttttgcgcca gcagctccag
     4921 aatacgcacg ttgtctgacc agcgcgttaa cttcggtttt tcatgagcgt agcgcaacac
     4981 cagatattgg gtaataaatt cgatatcggt aattccgcct tcatcagctt tgatatcaaa
     5041 gcgatcgcga tgtttattgc cgagatgagc gcgcattttc tcgcgcattt cccgcacttc
     5101 cgtttgcaga gttctacctt cacgcggcag cgtcataatc tcgcgacgca ctgcgtcaaa
     5161 gtgcgcggtg agctgcggat cgccgtacac tacccgcgca cgcaccagcg cctgatgttc
     5221 ccacgtccag gcctcgtttt tctgataatc ggcaaatgct tctgtggagg taaccagcat
     5281 tcccgccgcc ccggacgggc gcagccgcgc atccacttcg tacaaaatgc cggaagaggt
     5341 tcgcgtgctg aacaggtgca taatgcgttg cgccagacga agataaaact gtcgtccgtc
     5401 gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa tcatggagga agatcaggtc
     5461 aagatcggaa ctgtagccta actcccagcc gcccagcttg ccgtagccga ccactgcaaa
     5521 accacgccct tcgcgatcgt tgaggtgatt cggcttaccg tagcgggcaa ccatttgaac
     5581 ccacgcctgc tgaacgacgg catctatcat ggcttccgcc agccaggtta agtgatcgct
     5641 cactttcatc accggtagcg taccagcgat atccgctgcg gcgatgcgta acagctgcgc
     5701 ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg tcatcttccg gcacgcgcag
     5761 caaatactgg cgcaactcat cgcggtaggc atcggtcgcc gtcggctggt aaagagtgtt
     5821 tggatcgagc aattcatcca gcaataatgg ataacgcgcc agctggctgg caatcatcgg
     5881 cgatgcggca cacagagaaa tcaaatggtt aagcgccgca gggaattcac tgagcaattc
     5941 taaataggtg gtgcgggtaa caatccccac cagcaaggcg gtaatgcgcg acagcgtgac
     6001 agcagcgtct tcacgcgcac agacatcact tagcagatgc ggcatcagat ggtcgagcac
     6061 ctgacgtcct cgcggcccga tggtgcgctt atccagctct ttgcggaaat cggaaatcag
     6121 cgttagcacc tgtttgcgat catcctcgct aagatgcgcc agcactggcg tagtgtcatc
     6181 ttcctgcaac gcatcctgcc acagctcacg ccactgttcc gacagcgact cttcctgagt
     6241 ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg cgcacattgt tcatatgtgc
     6301 ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc atcgcccacg ccagccgcgc
     6361 acggttaagc tcatcagaag gaagcgtctg ggtttgttcg tcgttaatgc tttgcagcag
     6421 gttttccaga cgccgcagga acagatacgc cactcgcaat tgttcagcat cgttttcaga
     6481 aagcagatgc agcgcagcaa tggcgctgag cgttggcagt aaagagcacg attgcagcga
     6541 cggttcgcgc ccgccgcgaa tgagctggaa tacctggacg ataaattcaa tttcgcgaat
     6601 accacccgcg ccaagtttga tgttgtcggt caaaccgcgc cgacgcactt cacgggcaat
     6661 cattcctttc atgttgcgca gcgactgaat cacgctgaaa tcgatgtaac gacggaaaac
     6721 aaacgggcgt agcatcgcac gcaactcgtt agcatagacg ccttcgctat cgcccataat
     6781 ccgcgctttg accatcgcgt aacgctccca gtcgcgcccc tgctcctggt aataatcctc
     6841 caacgcggca aagctcagca ccagcgggcc actttcgcca aacggacgca gtcgcatatc
     6901 cacgcgatag acgaagccat cctgcgttgg ttgatccagc actttaatca gccgctgccc
     6961 catgcgggta aaaaactgcg cgttatccag ttcccggcgt ccgccctgcg tacaaccatg
     7021 ttccggccag gcaaaaatca gatcgatatc agaggagaaa ttcagttccc cgccgcccag
     7081 cttacccatg cctaagatca gcagcggttg cgcttcgccc tgcgcattgc acggcgttcc
     7141 ccactcgcgg cagcaggcgt catacagcca gtcacgcgcc gcaacaatca gcgtctccgc
     7201 cagataactg agctgctgca atatgctctc ttcagtaacc agtgccagcg tttgcgccca
     7261 tgcgatgcgc accataatgc gccgccggaa tagccgcagc tcgcgcatta acccggcttc
     7321 gtcactcaca ttactgagcg cctcctgcaa ccatgcagcg taatgctgcc attcatcggc
     7381 ctgcggcggt tggctttcca gttccgtcaa ccactctcga tgcgcaatta tgctgtcctg
     7441 cacaaaatca ctgaaagtaa gtactgactt cgcctgtgca cttagtggtt cctcggctaa
     7501 aggctctggc agccgctcaa caacggtctg ccagtactgc tgtaacggtg aagagagcgg
     7561 cttcataagc gattttatcc ttgcctgatt aacgttttcc gctgtgcaac cagaacggtt
     7621 cctgattgtt tgcctcatta cggaaatgct caatttcaat gcgttgcccg gtcgcaatag
     7681 cgtgacgcag cccctgccag ttctccagcc aggcatgcgc gacgacagga tcatagtaac
     7741 ccgccagcaa cagtattgag tcaatgtcac gcgtcaggcg tggcaactgg tcacggtaga
     7801 gatcgcctaa cggctggcag aaaacgcttt tcagttcagc ggcatggtgg gaaagatgga
     7861 tatcggcaaa gcgtttgaag gagtcgctga ttttgccctg cgctttggca tctaaaaatg
     7921 gctgccatgc tttgcttacc aaccattcgg tcaacgccag cttcgccatt gccgtttcgg
     7981 tagagtagac cgccgtcacg gcagaaaccg cagaagcaat ggtcgcttcg cattgggtca
     8041 gcaaatcacg taagtgagtg ctcgctttac gcggcacaat accaccgaac agcatcagcg
     8101 tatgacggac caggctaatg gctgccagca cctgttcttt cgccgcatcg ttaccgcgta
     8161 cccacagttc ttcatgatac tgccattgcg ctaacgccag ctcgagcgcc gcttccagcc
     8221 cctgttctac atcggctttt gccgcaacat gcaaaatggt ggtcggtttg atttcacgcg
     8281 ccggattgcc ctgcgccaga tgatagccac gcgccgcttt gctcaggctg ccctggcgta
     8341 atccggtttg cgataccagt tggttcgcca gtttcagcac cgcgcgcgtg tcgccgctaa
     8401 gcagttccag ttccagctca cagataggtt cagcaaattc ccccgctttc acctccccct
     8461 gatcgagagc gatttcaatt cggctgccat cgaccgccac cagccatttt tcgcgataaa
     8521 aatcggtgct gaacagcggt tgtacgcggg aagcgagatc ggcaggcaat tcaccgttcg
     8581 gccagacttc cgagggaaac tgcgccaggt cgagcgtcgg ttcgctcaac gccacgttat
     8641 attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc aactttcatg gtcatctcat
     8701 agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg cctacgcagc cagttatccg
     8761 gcgtttcgta gtaaatattc agcaactgca cggggtcatg gtgctcgccg cccagcgtat
     8821 tgagatggtc acgcaacgcc tcaacggcac tgtgattaac aataaacttt aattcgattt
     8881 cctgagccat ggcctttact tatcggttat gtcacataag tgacgatgaa cggcgaactt
     8941 aatgcgatct tttttgtcag tagatagtat tttgcgccaa attgccatgc aacgggcaat
     9001 ttgacgggcg taaaagtttg aagcagtggc aagacctgac acagaggatt ccgattgatg
     9061 tcgaatgctt tgcgtagcga cactgatgcc actactatcg ttccactttc catgacaata
     9121 acgacagcct gatgccaaaa ttacgcctga tcggattaac tttactcgca cttagcgcga
     9181 ctgccgtctc acacgctgaa gaaacgcgct atgtttctga cgaactgaat acctgggtcc
     9241 gtagcggtcc gggagatcat tatcgcctcg tgggcacggt taacgccggc gaggaagtga
     9301 ccttattaca aactgacgcc aacaccaatt atgcccaggt gaaagacagc tctggccgta
     9361 ccgcctggat cccgttgaaa caacttagca ctgagccaag cctgcgctcc cgtgtgccag
     9421 atctggaaaa tcaggtcaaa accctgaccg ataaactcac caatatcgat aacacctgga
     9481 atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag cgacagcgtg atcaacgggt
     9541 taaaagaaga aaatcagaaa ctgaaaaacg agctgattgt cgcgcagaaa aaggtcgatg
     9601 ccgccagcgt acagctggat gacaaacagc gcaccatcat catgcaatgg tttatgtatg
     9661 gcggtggcgt gctggggctt ggcttgctgc tcggtctggt actgccgcac ctgatcccaa
     9721 gccgcaaacg caaagatcgc tggatgaact aaatcgcctt cttcgttgca ctgtcttatt
     9781 attaggaatt gtgtatttga cgaagggatg atggcgtgaa gatttatctg gtcggtggtg
     9841 ctattcggga tgcattgtta gggctaccgg tcaaagacag agattgggtg gtagtcggca
     9901 gtacgccaca ggagatgctc gacgcgggct accagcaggt aggccgcgat tttcctgtgt
     9961 ttctacatcc gcaaacgcat gaagagtatg cgctggcacg taccgaacgg aaatccggtt
    10021 ccggttacac cgattttact tgctatgccg caccggatgt cacgctggaa gatgatctta
    10081 agcgtcgcga tctgaccatt aatgcgctgg cccaggacga taacggcgag attatcgacc
    10141 cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg ccatgtttcc cccgcttttg
    10201 gcgaagatcc gttacgcgta ctgcgcgtgg cgcgttttgc tgcgcgttat gcccacctcg
    10261 gttttcgtat tgccgatgaa actctgacgc tgatgcgcga gatgacccat gcgggtgaac
    10321 tggaacacct gacgcctgaa cgggtatgga aagagacgga aagcgccctc accacccgca
    10381 atccacaggt gttcttccag gtactgcgcg attgcggcgc actgcgcgtt ttattcccgg
    10441 aaattgacgc actgtttggc gttccggccc ctgccaggtg gcatccggaa atcgacacgg
    10501 gtattcatac cttaatgacg ctctcaatgg cggcgatgct gagtccgcag gtcgatgtcc
    10561 gtttcgcaac tttatgccac gatctcggta aaggactgac gccgccagaa ctttggccgc
    10621 gtcatcatgg tcacggtccg gcgggtgtga aattagtgga acaactctgc cagcgtctgc
    10681 gcgtgccaaa tgaaattcgc gatttagcca gactggtggc tgagtttcac gatctcatcc
    10741 acaccttccc aatgctgaac ccaaaaacta tcgtcaaatt gtttgattcc atcgacgcct
    10801 ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag cgaggctgac gtacgcggca
    10861 gaaccggttt tgaatcagcg gactacccgc aaggccgttg gttgcgcgaa gcctgggaag
    10921 tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg atttaaaggt gtggagattc
    10981 gcgaggagct gacccgacgg cggattgcgg cggtagccag ttggaaggaa caacgttgcc
    11041 caaagcctga ctgagtataa aactaaacgt taacacgaac catcccctcg ccccttcggg
    11101 gagagggtta gggtgagggg aacagaccag caccggtgcg aacaatcacc cccaccccgg
    11161 ccctctccct acagagcgag ggagaaaacc aggcctgctc ccgaaccttt tctcaatcaa
    11221 aagaacacga cgtacaccgc agccgccaca ataaagcgat aaatggcgaa cgggataaac
    11281 gaaatgcgct taatcaattg caggaaggtt ttaatcgcta tcagcgccac cacaaaagcg
    11341 gtgataaacc caacggcaaa catcgagata tcgccggttg tcaggaagcc ccagcttttg
    11401 tagagatcga gcgccgttgc gcccatcatc atcggtaccg ccagcaggaa cgagaactcg
    11461 gaagcagcgt aacggctcac gcccatcagc atcccacctg aaatggtggc cccggaacgg
    11521 gagaaacccg gccacagcgc cagacactgg aaacagccaa tcataaacgc ctgacgatag
    11581 gtcatatcat caagacccgg cgcacgcggc tctttcggct tcaggcattc ggcggcaatc
    11641 agcaacaaac cgccaacgac cagtgcatac atcacattta tcgggttaaa caatgactta
    11701 atcgtgtcgt ggaacaacag ccccagtacc accgccggaa tcatccccag caaaatgtgg
    11761 atcagcgtta aacgaccttt gctttctcct tcgtgctgca acgggcggcc aaagtggatg
    11821 ccaatcaggc caaacagacg ccgccagaac atcactacta ccgccagaat tgatcctaac
    11881 tggatcacaa cttcaaaggt tttcgccgtg tcgccctcga accccaacaa gtgaccgaca
    11941 ataatcatat ggcccgtgct ggataccggc agaaattctg tcaatccttc gaccacaccc
    12001 aatattgccg ctatcagcag cgagtgcata tcgctcatca ataaacccct aaattattaa
    12061 aatgtaccgc ttgtccggac tactgcagta agaccaggtt ataaccgttt ggtttaacag
    12121 ctgtaaaatt aattattttc tttcagatta ttgccacgct caatgattac accaacattc
    12181 gccgcccgcg ccattgcgcc tggcttgctg agtttgatgc gcacccacgg cgagttgaag
    12241 cgtgctaaca gcagctccgc cacctcttca gccacgcgtt ccaccagcgc aaaacgcgcc
    12301 ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt aactgaggca atccgccaca
    12361 tcatcacttt tcgccgcttt acggttatcc catgccattt cgatatcgaa cactaacttc
    12421 tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg tgattaccga aagttgctct
    12481 ataaatacaa tatccatcac gtcctgcctg ctttttggct aaccggatac cacttccggc
    12541 gaaatgtgcg tattatccac agattcatcg ttgaacacga attttcaaaa cggaacagct
    12601 tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct ccatttccag
    12661 tgccattctg gtttgccgct tgtgtgggct gcccgatccg cgaaccagcg gctccggcaa
    12721 tccaggcgca accaatgtgt tacgtatcgg tggcaaggga gcagccgtag cagtactgat
    12781 tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag gtgtcagccc
    12841 cttctggtta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc ccgttttctt
    12901 cggatttaaa ggaggaaaag gcgttgctac cgcttttggt gccatcgcac ccattggctg
    12961 ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga gcggatactc
    13021 gtcgctggga gcgattgtca gtgcactgat tgctccgttt tatgtctggt ggtttaagcc
    13081 acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgtc atcatgacaa
    13141 catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca aaagaaagcg
    13201 cgaaaaggat cccgagtgat ttctggtgga tctacatgac ctgatagcct tcatcgggct
    13261 tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt aatttcatgc
    13321 tgcgatagag cggctcccgg taaacagccc agatattggc gctttgtgca tactccggca
    13381 atacttgcac cagtttgcca ctctccagaa acggcaacac atcccactcg gaacgcagca
    13441 taatcccttt gccctccagc gcccattgca acactatctc gccgctattg gaggaaagat
    13501 gcccgctaac cttcaccgat tttttctcct gaccgttccc caactcccat attccgtggg
    13561 tcatatcgcg ctctttggtc accagacagt catgacgact taattcctgt aatgattgtg
    13621 gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta tttttcgtta
    13681 acagatgcgc aatataataa tcaggaattt cgtcattaat acgaatatcc agatcaatat
    13741 tatcctgcac taaatcaatt tgccgatcga acaattcaaa atgcacctgt aactcaggat
    13801 aattgcgcat aagttcggta atggctggcg caatatggct gcgcccaaaa ccaaaactgc
    13861 aaccaatacg aatcatcccc tccgggcgcg ttttgatttg cgtgacgtca tcgaccagtc
    13921 gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca ctttcagtca
    13981 gcgccacgcc gcgagcggag cggttcagca gcgttgttgc cagcgtattt tcaaggattt
    14041 ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt cgccgctgcg gcgctaaaac
    14101 tcccgctgtg gacgatttct accagtacct gcaagtcttt ggctaaaggc cagctattca
    14161 gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat ttcgcaaaac attgccattc
    14221 cctgctctgg cgcaatattc attcttattt tcgcgttgat tcacgaattg ttaattacat
    14281 attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg cggtgaattt
    14341 ttccagacaa atacaaaaac tggagttgcc atgatgagcg aaagtaataa gcaacaggca
    14401 gtgaataagt tgacggagat tgtcgctaac tttaccgcca tgatttctac ccgaatgcct
    14461 gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacgtcgtc gatggggaaa
    14521 attatctacc atacgatgtt cgacaacatg caaaaagcga ttgacctgaa tcgtcctgcc
    14581 tgtcaggaca ccggggagat tatgttcttc gttaaagtcg gttcccgctt cccactgctt
    14641 ggcgagctgc aaagcatact caaacaagcc gtggaagagg caaccgtcaa agcgccacta
    14701 cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac cggtagcggc
    14761 gtaccgtggg tcacctggga catcatcccc gacaatgacg atgcggaaat cgaagtttac
    14821 atggcaggcg gcggctgcac gctacctggc cgctcgaaag tgttaatgcc gtcagaaggc
    14881 tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt aaacgcctgt
    14941 ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc cgtactctcg
    15001 cgtaaagcca ttttgcgccc gattggcagc cgccatccca atccaaaagc ggcagaactg
    15061 gagctacgcc tggaagaagg actcaaccgt ctggggattg gtccacaagg gctgactggc
    15121 aacagttcag tgatgggcgt acatatcgaa tctgccgccc gccatccgtc aaccatcggc
    15181 gttgctgtct ctaccggctg ctgggcgcat cgtcgcggca cactgctggt tcatgccgat
    15241 ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc ctgacaaccc
    15301 cgatcaaagc tgaagatctg caagatattc gcgtcgacga tgtgatctac ctgaccggta
    15361 cgctggtgac ctgccgtgac gtttgtcacc gccgcttgat cgaactgaaa cgtccgatcc
    15421 cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc aaaaacggcg
    15481 ataaatggga gatggtttcc gttggcccga caaccagtat gcgtatggaa agttttgaac
    15541 gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg caaaggcgga atggggccgc
    15601 tgaccgaaga aggctgccag aaattcaagg cgctacatgt gattttcccg gcaggctgcg
    15661 cggtgctggc ggcaacccag gtggaagaga ttgaagaagt gcactggaca gagctcggaa
    15721 tgccggagtc gctgtgggtc tgtcgggtca aagagttcgg cccgctgatt gtctctattg
    15781 atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa cgccgcgatc
    15841 ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc cggagaggct
    15901 cgccccctct cctttttcgc aggcataaca cgatgaaacc ttccactgaa tggtggcgat
    15961 accttgcgcc gctggcggtc atcgccatta ttgctctaat tccggttccc gcagggctgg
    16021 agagtcatac ctggctctac tttgccgttt ttactggcgt gatcgttgga ctgatcctcg
    16081 aacccgtgcc gggtgccgtg gtggcgatgg tgggtatatc catcatcgcc atactctctc
    16141 cctggctgct gttcagcccg gagcagctcg ctcagccagg ctttaaattc actgcaaaat
    16201 ccctctcgtg ggccgtttcc ggtttttcta attcggttat ctggctgatt ttcgccgcct
    16261 ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg ccgcattgcg ctgattctgg
    16321 tgaaaaagat gggacatcgc acgctgtttc tcggctatgc ggtgatgttc tccgagctga
    16381 tcctcgcacc tgtaacaccg tccaactcgg cgcgtggtgc ggggattatc tatcccatca
    16441 tccgtaacct gccaccgctc tatcaatcac aaccaaacga cagcagttcg cgcagcattg
    16501 gctcgtacat catgtggatg gggattgttg ccgactgcgt gaccagcgcc attttcttga
    16561 cggcgatggc accaaacttg ctgttaattg gactgatgaa aagcgcatct cacgccacgc
    16621 tgagttgggg cgactggttc ctcgggatgt tgccgctcag tattttgctg gtgctgctgg
    16681 ttccctggct ggcttatgtg ctgtacccgc cggtactgaa gtctggcgat caggtgccgc
    16741 gctgggcaga gacggaactg caggcaatgg gcccgctctg ttcgcgtgaa aaacggatgc
    16801 tggggctgat agtaggcgcg ctggtgctgt ggattttcgg cggtgattat atcgatgccg
    16861 cgatggtcgg ttacagcgtg gtggcactga tgctgcttct gcgcattatc agttgggacg
    16921 acattgtcag taataaagcg gcgtggaacg ttttcttctg gctggcctcg cttatcaccc
    16981 tcgctaccgg actcaacaac accggtttta ttagctggtt tggcaaactg ttagcaggca
    17041 gcttaagcgg ttattcgcca acgatggtga tggtggcgtt gattgtggtg ttttatctgc
    17101 tgcgctactt tttcgccagc gccacggcgt atacctccgc cctcgcaccg atgatgattg
    17161 ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg gttggcgcgg
    17221 caattggtct ggggagcatt cttacaccat acgccaccgg acccagtccg atttactacg
    17281 gtagtggtta tctgccaacg gcggattact ggcgactggg ggcgattttt gggctgatat
    17341 tcctcgtgtt gctggtgatt accggcttac tgtggatgcc cgtggtgttg ctttaagtga
    17401 ggacggtgcc ggaaattgct ctccggcacc caatagtttt acgcagccgg taactccgcc
    17461 agcggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc tttaaaccgc
    17521 accattccgg cataggcgat catcgcgccg ttatcagtac aaaactccgg acgcgcgtag
    17581 aacacttcgc cgcggcgttt tttcatcatt tcagccagct tcgcccgtaa cgtgcggtta
    17641 gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa cgctcgctta
    17701 cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat atcagcacgc
    17761 gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt cagaccagaa
    17821 aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa gcgcccggca
    17881 gtaccctgag ccgccatttt cgacagtaac ggtccgccag gataatccag ccccagcagc
    17941 ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc gagcagctcg
    18001 tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggacac cagcagcgcg
    18061 acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg cccttccata
    18121 tggtgtaccg ggattgccgg aacgttccag gcaaacgcca gagaacgccc cacggttgcg
    18181 ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc atcaatgtct
    18241 tttgccgtta agccagactc ctttagcgcc gcctggatca acggtacggt tttgcgcacg
    18301 tgatcgcggg aggccagttc aggcacgacg ccgccatagt cagcgtgcaa tttcacctga
    18361 ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat gccggtttca
    18421 tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct ttattaccgc
    18481 gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag aaagcctcgt
    18541 gtatactcct cacccttata aaagtccctt tcaaaaaggc cgcggtgctt tacaaagcag
    18601 cagcaattgc agtaaaattc cgcaccattt tgaaataagc tggcgttgat gccagcggca
    18661 aaccgaatta atcaaaggtg agaggcacat gccggtaatt aaagtacgtg aaaacgagcc
    18721 gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg ttctggcgga
    18781 agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg ctaaagcttc
    18841 tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca ctcgtctgta
    18901 ctaattcccc gagagcgttg ctctccgatc agaccgagtt gtagttgtaa ggccgtgctt
    18961 ccgaaaggaa tgcgcggctt attttcgttt atgaattgct aaaaatcggg gcctatggct
    19021 ggacgaatcc cacgcgtatt cattaatgat ctgctggcac gcactgacat cgtcgatctg
    19081 atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg ttgtccattc
    19141 cacaacgaga aaaccccgtc attcaccgtt aacggtgaga aacagtttta ccactgcttt
    19201 ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga actacgacaa gctcgagttc
    19261 gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg aagtgccatt tgaagcaggc
    19321 agcggcccca gccagatcga gcgccatcag aggcaaacgc tttatcagtt gatggacggt
    19381 ctgaatacgt tttaccaaca atctttacaa caacctgttg ccacgtctgc gcgccagtat
    19441 ctggaaaaac gcggattaag ccacgaggtt atcgctcgct ttgcgattgg ttttgcgccc
    19501 cccggctggg acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg ccagtcattg
    19561 attgatgcgg ggatgttggt cactaacgat cagggacgca gttacgatcg tttccgcgag
    19621 cgggtgatgt tccccattcg cgataaacgc ggtcgggtga ttggttttgg cgggcgcgtg
    19681 ctgggcaacg atacccccaa atacctgaac tcgccggaaa cagacatttt ccataaaggc
    19741 cgccagcttt acggtcttta tgaagcgcag caggataacg ctgaacccaa tcgtctgctt
    19801 gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat acggcattaa ttacgccgtt
    19861 gcgtcgttag gtacgtcaac caccgccgat cacatacaac tgttgttccg cgcgaccaac
    19921 aatgtcattt gctgttatga cggcgaccgt gcaggccgcg atgccgcatg gcgagcgctg
    19981 gaaacggcgc tgccttacat gacagacggc cgtcagctac gctttatgtt tttgccggat
    20041 ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag aagcgtttga agcgcggatg
    20101 gagcaggcga tgccgctttc cgcatttctg tttaacagcc tgatgccgca agttgatctg
    20161 agtacccctg acgggcgcgc acgtttgagt acgctggcac tgccattgat atcgcaagtg
    20221 ccgggcgaaa cgctgcgaat atatcttcgt caggaattag gcaacaaatt aggcatactt
    20281 gatgacagcc agcttgaacg attaatgcca aaagcggcag agagcggcgt ttctcgccct
    20341 gttccgcagc taaaacgcac gaccatgcgt atacttatag ggttgctggt gcaaaatcca
    20401 gaattagcga cgttggtccc gccgcttgag aatctggatg aaaataagct ccctggactt
    20461 ggcttattca gagaactggt caacacttgt ctctcccagc caggtctgac caccgggcaa
    20521 cttttagagc actatcgtgg tacaaataat gctgccaccc ttgaaaaact gtcgatgtgg
    20581 gacgatatag cagataagaa tattgctgag caaaccttca ccgactcact caaccatatg
    20641 tttgattcgc tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg cacgcatggt
    20701 ttaagcaacg aagaacgcct ggagctctgg acattaaacc aggagctggc gaaaaagtga
    20761 tttaacggct taagtgccga agagcgatcg ggaagccccc gacagccgca ctgagaggca
    20821 gcggcaaata tataagtacg ccctcgtaat tatcgttggc ggtaaacaac cgttggattt
    20881 cagcgttaac ggctgaagga catcgggtca atcgcccaac accaacctca tgaaataagt
    20941 gtggataccg tcttatggag caaaacccgc agtcacagct gaaacttctt gtcacccgtg
    21001 gtaaggagca aggctatctg acctatgccg aggtcaatga ccatctgccg gaagatatcg
    21061 tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc attcaggtga
    21121 tggaagaagc accggatgcc gatgatctga tgctggctga aaacaccgcg gacgaagatg
    21181 ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg cgcacgactg
    21241 acccggtacg catgtacatg cgtgaaatgg gcaccgttga actgttgacc cgcgaaggcg
    21301 aaattgacat cgctaagcgt attgaagacg ggatcaacca ggttcaatgc tccgttgctg
    21361 aatatccgga agcgatcacc tatctgctgg aacagtacga tcgtgttgaa gcagaagaag
    21421 cgcgtctgtc cgatctgatc accggctttg ttgacccgaa cgcagaagaa gatctggcac
    21481 ctaccgccac tcacgtcggt tctgagcttt cccaggaaga tctggacgat gacgaagatg
    21541 aagacgaaga agatggcgat gacgacagcg ccgatgatga caacagcatc gacccggaac
    21601 tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt tgtaacgcgt gacaccatca
    21661 aagcgaaagg tcgcagtcac gctgccgctc aggaagagat cctgaaactg tctgaagtat
    21721 tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc atgcgcgtca
    21781 tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt gagcagtgca
    21841 aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc gatacctggt
    21901 tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa actgcacgat gtctctgaag
    21961 aagtgcatcg cgccctgcag aaactgcagc agattgaaga agaaaccggc ctgaccatcg
    22021 agcaggttaa agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc cgccgtgcga
    22081 agaaagagat ggttgaagcg aacttacgtc tggttatttc tatcgccaag aaatacacca
    22141 accgtggctt gcagttcctt gacctgattc aggaaggcaa catcggtctg atgaaagcgg
    22201 ttgataaatt cgaataccgc cgtggttaca agttctccac ctacgcaacc tggtggatcc
    22261 gtcaggcgat cacccgttct atcgcggatc aggcgcgcac catccgtatt ccggtgcata
    22321 tgattgagac tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa gagatgggcc
    22381 gtgaaccgac gccggaagaa ctggctgaac gtatgctgat gccggaagac aagatccgca
    22441 aagtgctgaa gatcgccaaa gagccaatct ccatggaaac gccgatcggt gatgatgaag
    22501 attcgcatct gggggatttc atcgaggata ccacccttga gctgccgctg gattctgcga
    22561 ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc gcgcgtgaag
    22621 cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg ctggaagaag
    22681 tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg aaggcgctgc
    22741 gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag cttcctggac gattaatcgg
    22801 taggccggat caggcgttac gccgcacccg gcactagtcc ctctgcacaa acgccacctt
    22861 tt
//
LOCUS       DAZRVY010000059        22378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZRVY010000059 DAZRVY010000000
VERSION     DAZRVY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.059"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            35..637
                     /locus_tag="ACHXOX_002357"
     CDS             35..637
                     /locus_tag="ACHXOX_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005804.1"
                     /translation="MLINQQERDELELALSCTDHEYRLPVKHKHLKTDYTTSGFSREE
                     AKEILIEFYRDNGYTDVHSFFKKHRTSHTEFRRVRDWFDFDIKRYYRIDDGPIYRLQW
                     KPIREVLKQKRLNHAITRYRNEAFKKGYGDTRELFVELANVRYSHYYNDPKGFFEVLR
                     KVDISRGTYYSRLKKYEIKAEFFMSVDDGELFPIKCKSSK"
     gene            673..900
                     /locus_tag="ACHXOX_002358"
     CDS             673..900
                     /locus_tag="ACHXOX_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005805.1"
                     /translation="MTTKATRGRPARFNAEQMADIAYAYYTADRGKEAKEQILSEHGI
                     SIAQFYKNMKKLDIKFYVQIGDGQIVEATGF"
     gene            complement(981..1793)
                     /locus_tag="ACHXOX_002359"
     CDS             complement(981..1793)
                     /locus_tag="ACHXOX_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR7005806.1"
                     /translation="MNTVTVMNTAKTASVSLVVDNTKPLTMSSRQIAEFTGKEHKNVK
                     RDIKSMLDALELDVLSFERTYLDQCGREQTEYHLDEEMTLTLVTGYDVKRRMVVIQEW
                     KRLKEENEKLRQERLAVNPYADFEETDWIELALKKTRENKKLIELHVRKSVDSHSMTR
                     LLGAKKGATKVKEALTALRTAGYIERVYDENNKPCGYVANDSSLSFCRMNAHYQLEFT
                     VDVLPVLVELGVLEEEQKATLNLPKPNNTILIQNKAAERLRQNVGSLEVFGL"
     gene            complement(1883..2215)
                     /locus_tag="ACHXOX_002360"
     CDS             complement(1883..2215)
                     /locus_tag="ACHXOX_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005807.1"
                     /translation="MKEFTITKSLYDWTVKISGKTVSVTPSHVENDWGVSRETISRSM
                     HVPFDLMTLTGIHAMLEVREQFEAGNHNATPVNNHLLTNEEREIIENWRYEYAIPYYE
                     EDQVEIDW"
     gene            complement(2336..2803)
                     /locus_tag="ACHXOX_002361"
     CDS             complement(2336..2803)
                     /locus_tag="ACHXOX_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005808.1"
                     /translation="MNHKMTTTTPFILLGFDADSFHNDKLMKMFRERGYNILFNSDES
                     HFSSAWYAFCRNKKPTIVVNCFTDEERLKYQDLPAIIVRDDLPFEVRSGDIDLGSFVT
                     VYELDEAMFNYGKETAQEAPEDDEQDEDDEDYAPICFSSQNGFYGYDEGLDML"
     gene            4202..4381
                     /locus_tag="ACHXOX_002362"
     CDS             4202..4381
                     /locus_tag="ACHXOX_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005809.1"
                     /translation="MARPVKFTREMIVDIAKRFYSDTERGGENFFKKEGIDRSGFYKL
                     MKKYEIQVKINVQVC"
     gene            4499..5443
                     /locus_tag="ACHXOX_002363"
     CDS             4499..5443
                     /locus_tag="ACHXOX_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005810.1"
                     /translation="MLFSANFFTNEVTNAKAVIKFGTEANKKIQPKVVAHVSFAQQKS
                     AFAGFKAHREALIAAGFGVDGKLPNGIRKAIDRVKPAKDVAVDHIVTFTVKNSSELFH
                     LVIAGDTAIVSAPNMDFQDTADCNITFGRRKEVTGKAKLNFTKSGNPYFAVFFNSHAT
                     EKEVVELVAYEGEEKARIESCDAFVELEAQVNAVSPVAVEDRFSALEAQIAELKAALK
                     EKDQIIEAQKAEIEELKAKEVAPVAVVENAADEAANDEVEVKIDVMRDPMAALQLLRS
                     FKGKSHFSSDAVDEEEEQKRNVAMLHDEMYGTNFSYCQ"
     gene            complement(6153..6707)
                     /locus_tag="ACHXOX_002364"
     CDS             complement(6153..6707)
                     /locus_tag="ACHXOX_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005811.1"
                     /translation="MKEELTLTEISRLYGYSLNGGGKRWLERGLPYNTNTRRVPAKEG
                     CDWVLKNVINPLKQTSIKEAIDQERLRRERAMADAAERENQEAMNLLIPVGCVEQELA
                     EYCGKVKQTILQIATIDALEILESANDQKTLKNKLREIIERRLNEVGDLFENADLGED
                     EEEELPLMDEPEEEPEEDDEFDVS"
     gene            complement(7051..7455)
                     /gene="ycdX"
                     /locus_tag="ACHXOX_002365"
                     /pseudo
     CDS             complement(7051..7455)
                     /gene="ycdX"
                     /locus_tag="ACHXOX_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
     gene            complement(7510..8448)
                     /gene="ghrA"
                     /locus_tag="ACHXOX_002366"
     CDS             complement(7510..8448)
                     /gene="ghrA"
                     /locus_tag="ACHXOX_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR7005812.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            8682..8769
                     /locus_tag="ACHXOX_002367"
     tRNA            8682..8769
                     /locus_tag="ACHXOX_002367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8716..8718,aa:Ser,seq:gga)
     gene            8814..8950
                     /locus_tag="ACHXOX_002368"
     ncRNA           8814..8950
                     /ncRNA_class="other"
                     /locus_tag="ACHXOX_002368"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(9041..9727)
                     /locus_tag="ACHXOX_002369"
     CDS             complement(9041..9727)
                     /locus_tag="ACHXOX_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR7005813.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(10308..11096)
                     /gene="phoH"
                     /locus_tag="ACHXOX_002370"
     CDS             complement(10308..11096)
                     /gene="phoH"
                     /locus_tag="ACHXOX_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR7005814.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(11717..12988)
                     /gene="efeB"
                     /locus_tag="ACHXOX_002371"
     CDS             complement(11717..12988)
                     /gene="efeB"
                     /locus_tag="ACHXOX_002371"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR7005815.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(12994..14121)
                     /gene="efeO"
                     /locus_tag="ACHXOX_002372"
     CDS             complement(12994..14121)
                     /gene="efeO"
                     /locus_tag="ACHXOX_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR7005816.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(14179..15009)
                     /gene="efeU"
                     /locus_tag="ACHXOX_002373"
     CDS             complement(14179..15009)
                     /gene="efeU"
                     /locus_tag="ACHXOX_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR7005817.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(15675..17183)
                     /gene="putP"
                     /locus_tag="ACHXOX_002374"
     CDS             complement(15675..17183)
                     /gene="putP"
                     /locus_tag="ACHXOX_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR7005818.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            17605..21567
                     /gene="putA"
                     /locus_tag="ACHXOX_002375"
     CDS             17605..21567
                     /gene="putA"
                     /locus_tag="ACHXOX_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7005819.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(21607..22245)
                     /gene="rutR"
                     /locus_tag="ACHXOX_002376"
     CDS             complement(21607..22245)
                     /gene="rutR"
                     /locus_tag="ACHXOX_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR7005820.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         5961 a         5516 c         5204 g         5697 t
ORIGIN      
        1 gataaatacg ggtattaaga tttaaggagg gtgtatgcta attaatcagc aagaaagaga
       61 tgaattagaa ttagccttgt cttgtaccga tcatgaatat cgtttgccag taaaacataa
      121 gcacttaaag actgattaca caacatccgg ttttagccgt gaggaagcga aagagatttt
      181 aatagaattt taccgtgata atggttatac agacgttcac agcttcttta agaagcatag
      241 aacatcacat acagagtttc ggagagtccg agattggttt gattttgata tcaagcgtta
      301 ttatcgcatt gatgacggtc ctatctatcg gttgcagtgg aagccgatcc gagaagtgtt
      361 gaaacagaag agattgaatc acgctattac acgctatcgt aacgaggctt tcaagaaagg
      421 ctatggtgat acaagggaat tgtttgtaga actcgctaac gtgcgataca gccactatta
      481 caacgatcct aaagggttct tcgaagtgct tcgcaaggtt gatattagtc gtggtacata
      541 ctattcacgg ttgaagaagt acgagattaa ggcagagttc tttatgtcgg ttgacgatgg
      601 agaacttttt ccgataaaat gtaagtcctc taaataaagg tgaacattca ctttaatttg
      661 gagaaaatga ctatgactac taaagctact cgcggtcgcc ctgcccgttt taacgctgaa
      721 cagatggctg atattgccta cgcttactat acagcggatc gtggtaagga agcgaaagaa
      781 cagattctta gtgaacatgg gatctcaatc gcccaattct ataagaatat gaagaagtta
      841 gacattaaat tctatgttca gatcggcgat ggtcagatcg tagaagcaac aggtttttga
      901 ttctctttgc cactcttcgg agtggctttt ttgtatctgt aggtatgaaa aagccccgca
      961 cgaggcgagg ctaaaaatta ttacagtccg aagacttcca gtgatccaac attctggcgg
     1021 agacgttcag ccgctttatt ttgaatcaga atagtgttgt taggcttagg aagattcaga
     1081 gttgcttttt gttcttcttc cagaacaccc aattcaacca gcaccggaag cacatcaaca
     1141 gtaaactcaa gctgataatg agcattcatg cggcagaaag acagagaaga gtcattagca
     1201 acatacccac acggcttgtt attttcatca tagacgcgct caatatatcc agcagtacgt
     1261 aaggccgtca gtgcttcttt aaccttcgta gcgccctttt tagcgcctaa cagacgggtc
     1321 attgaatggc tgtctacgga tttacgaaca tgaagttcaa tcagcttttt attttcgcga
     1381 gttttcttta atgccagttc gatccagtcg gtttcttcaa agtctgcata cgggttaacg
     1441 gctaaacgtt cctgacgcag tttttcattt tcttctttca gtcgtttcca ttcctgaata
     1501 acaaccattc gacgcttaac atcgtaacca gtgacaagag tcagcgtcat ttcttcgtct
     1561 aagtgatatt cggtttgttc acgtccacac tgatcaagat aggtgcgctc aaaactgagc
     1621 acatctaatt ccagagcatc gagcatggat ttaatatcac gtttaacgtt tttatgctct
     1681 tttccggtaa attcagcgat ctgacgagaa gacatggtca gcggtttggt gttatcaaca
     1741 accagagaca cggaagcagt tttagcagta ttcataacgg taacagtatt catagtatta
     1801 attcccctta aacggtttaa tgatttggtc ctcctagttc cggcagcatc accggaacat
     1861 ttttatttat gatagaaatt tattaccaat cgatttctac ttggtcttct tcataataag
     1921 gaatcgcata ttcataacgc caattttcga tgatttcacg ttcttcatta gtaagaaggt
     1981 gattgttaac tggagtcgca ttgtgattac cagcttcgaa ttgctcgcga acttccagca
     2041 ttgcatgaat accagttaag gtcatcagat caaacggaac atgcatacta cgggagatag
     2101 tttcacggga tacaccccaa tcattttcta catggctagg ggttacagaa acggttttgc
     2161 cggagatttt aacagtccag tcatacaggg atttagtgat ggtaaattct ttcatgttac
     2221 atcctcttta caaatctagg tcaaataggt tcaaagttgt caagtgcgat aatcataaaa
     2281 aagtcagaaa tttttgtcaa taccccttga caagacgcca aagggttgac ctaacttaca
     2341 gcatatctaa gccttcatcg taaccgtaga agccattctg agaagagaaa cagattggag
     2401 cgtagtcttc gtcatcttcg tcttgttcgt catcttccgg ggcttcctga gcggtttctt
     2461 tgccgtagtt aaacattgct tcatccagtt catagacagt aacgaagcta cccaggtcaa
     2521 tgtcaccgga acggacttca aacggaagat catcacggac aataattgct ggtagatcct
     2581 ggtactttaa gcgttcttca tcggtgaagc agttaacaac gatggtcggc ttcttattac
     2641 ggcaaaacgc ataccatgca ctagagaaat gagattcatc agagttgaaa agaatgttgt
     2701 aaccacgttc acggaacatc ttcattaact tgtcattgtg gaagctatca gcatcaaaac
     2761 ctaacaggat gaaaggagta gtggtagtca ttttatggtt catagtggaa atcctctaaa
     2821 agaaagtgaa tcaatctaat attatttata aagagaaaat tcttgttatt atgaaaggtg
     2881 attgtattca gtcgggctaa agccctcctt catgatttga ttaaaagtac atcaaaacga
     2941 agaagtatct ttagaatata agaatctttc ttttggagaa ataataatca ggaagcaatt
     3001 tagcaaccga aggttgcaat cccgaaggga taccaatacc tttctagaaa gtgattgtta
     3061 ctcacttcgt tcgtttacta gaatcagtta tcatctagta ttaaaatccc tttctaagaa
     3121 aatcctttca aaagtgattc tcgatgccac gcagtggcgt cgcagacgtt tgagagtgag
     3181 cgaagcgagc gagcaaacta gttaattcct tctaagacca tctagtatca tctagtttaa
     3241 attagttgtt actagaagta attggttagc gctacgcgca gtcgggcaaa gccctcctaa
     3301 tatctttctg gttatttgtt gtttttagtt ttacacatga gcgtagcgaa tgtgttatat
     3361 aatatctatg tacgccgcat cacccgcttt taccactcgc taagttaatg ttttataaag
     3421 agtttttctc accaatcctt ttcgggggca aatttcaggg gcaaaaatta gattttcagg
     3481 ggcaattatt ggcatttcag gggcaaaaat gaggggcaaa atcactgttt cccagaattt
     3541 caggggcaaa aatagtaggt ttcaggggca aaaataggat agtagaatct tggggatttt
     3601 attcgggggc aaaaattcag aatttcaggg gcaattccca tcaaatattt tgatagaaaa
     3661 ttcttgtata ttatttaatc aaaaaatcac caaaaagtgg cgtaagttaa taatttaaaa
     3721 agaattgtca aaaggtctcc gtgctataat aggttataaa ggttgataat cctgatctac
     3781 cattttcaaa caaaaatttt gatagtcatt tctcgcttag atcaagtgta ttgacaactg
     3841 tcaaggatta ttttatactt tttctcactg gtgggggttg aaattttcta aacagtcccc
     3901 atatagatga taagcaagaa tttccacgtt ataaatacat gtgtgaaatg attgttcact
     3961 tctgaaaatc tcctgattca ctgctaattc atacagcact ggttagatat acagtgcttt
     4021 ttcggttgaa tattgtacga attgattaat agttaatctt ctctttagca attcgtgctc
     4081 ccactgatcg gatatacagt ggttttacag ggttttctga aaatcatttt ataaatacaa
     4141 gtgttcagag cgaaatgctc ttttttaaga aattattaat ccacatgtga ggaatcgact
     4201 gatggctaga cctgttaagt tcactcgtga aatgattgtt gatattgcta aacgctttta
     4261 cagcgacact gagcgaggcg gggaaaactt ctttaagaag gaaggaattg atagaagcgg
     4321 attctacaag ctaatgaaga aatacgaaat tcaggtaaag attaacgttc aagtttgtta
     4381 atccactttc actgagaatt atatgatggc tttaaaaata aaaaagcctc cctgttggcg
     4441 caaggaggct tttgatatga aacattaaaa ctaacacaga gtattcagga gtatttatat
     4501 gttattttcc gctaactttt tcactaacga agtaaccaac gctaaagcag ttatcaaatt
     4561 tggcaccgaa gcaaacaaga agattcagcc gaaagttgtt gctcatgttt cctttgcgca
     4621 gcagaaatcc gctttcgctg gctttaaagc acatcgtgaa gcactgatcg cggctggctt
     4681 tggtgttgac ggtaaactgc ctaacggcat tcgtaaagca atcgaccgcg ttaaacctgc
     4741 taaagacgtt gcagtagacc acatcgttac tttcaccgtt aagaactctt ctgaactgtt
     4801 ccacctagtg attgctggtg atactgctat cgtttccgct cctaacatgg attttcagga
     4861 taccgctgat tgcaacatta cctttggtcg tcgtaaagaa gttaccggaa aagctaaact
     4921 gaacttcacc aaatccggca atccttactt tgctgtattc tttaacagcc acgctactga
     4981 gaaagaagta gtagaactgg ttgcatatga aggtgaagaa aaagcacgta ttgaatcttg
     5041 cgacgctttc gtagaactgg aagcacaggt taatgctgtt tctccggttg ctgttgaaga
     5101 tcgtttctct gcactggaag cacagatcgc agaactgaaa gctgcactga aagaaaaaga
     5161 ccagatcatc gaagcacaga aagctgagat tgaagaactg aaagctaaag aagttgctcc
     5221 ggtggcagta gttgaaaatg cggctgatga ggccgcaaat gatgaagtag aagtgaaaat
     5281 cgatgtgatg cgtgatccga tggctgcact gcaattgctt cgttctttta agggcaaatc
     5341 tcatttttct agtgatgcgg tagacgagga agaagaacag aaacgcaatg ttgcaatgct
     5401 tcatgatgaa atgtacggta ctaacttttc ttactgccaa taaaaaaggg actccgaaga
     5461 gtccctagta agtttaggtt aggtttgcac aacaaagatg aggttataat gaacataatg
     5521 ctacattatt ctttaaagcc agaaaaataa ccgcctcttt cgaggcggga ggattaacac
     5581 tcaatctata cttgttatgt ttttatgttg tatagatcac tgactatgaa aacactgtac
     5641 tggatgtgtt ttatggcttt taatcgggaa tcctcaaaga agatcccctg ttaataacca
     5701 taaggagaaa aggcagatta cactattatt tagtatcctt tttacagcaa ctaaaaagaa
     5761 taaacaaaaa gaagcatacc cagccgtaag gcgtaccgat tagaaatttg aaaaatacca
     5821 ccagcgatat aaaaatcaat ttaatgccag cgacgatagt caaaaacagc agagtagcaa
     5881 agtagacaaa aagatccata gataatcctc tttgaaaacg accatcatct atagttcaat
     5941 tcatcgattt aattaatgat acttgaaagc gttacacaaa gtagaaatgc caatatgtgc
     6001 cagtgaatat acgatagtta atcctacagc cagataaatc aaaaattcaa ccataggtaa
     6061 atcctcaaat aagaaaaagg ttagcggggg atgttctccc ccgtagacgt tgaccatcaa
     6121 catctttcac atatgtattt agtatagaaa ttttaagaca catcaaattc atcgtcttct
     6181 tctggttctt cttccggttc atccattaat ggtaattctt cttcctcgtc ttctcccaaa
     6241 tcagcatttt cgaacaagtc ccctacttca tttaaacgac gctcgatgat ctcccttagc
     6301 ttattcttca atgttttctg gtcattggca gattcgagaa tttctaaagc gtcgattgtg
     6361 gcgatctgca aaatagtctg cttcactttt ccgcaatatt ccgctagttc ctgttctaca
     6421 cacccgaccg gaatcaggag attcattgct tcctggttct ctcgttcggc tgcatctgcc
     6481 attgctcttt ctctgcggag acgttcttga tcaatcgctt ctttgattga agtttgttta
     6541 agtgggttaa ttacgttctt tagcacccaa tcgcaacctt cctttgccgg aacacgcctc
     6601 gtattggtat tgtaaggtag tcccctttct aaccatcgct taccgccacc attcaatgaa
     6661 tagccgtata aacgggaaat ttctgtaagg gttaattctt ctttcattgg ttatcctcca
     6721 ttaaatttgc gtaattttat ttattggttt tacgcaaaat ctaagcgcct aaaacacgtt
     6781 ttaacgaagg gaaagatcca gtaatggtaa gggctaggaa ggggggttaa tagtcgcgta
     6841 gcgaccgcgc taggactgca ctgatcacgt tttaaaatct cacacgcaca tcaaataccg
     6901 gggtgccgaa aactcccttt atttcagccg tggaacagta ccttttgatt ttgtcaagtc
     6961 tttttttgaa attttttcgt tcaatcgctc actaatcaat caaacgattc accaaataaa
     7021 ttttttagtt attggggggt tgactacgtt cgggtttccg ggatggctta ttatatgcac
     7081 attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt catgtggtgc
     7141 aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca ttttaccgct
     7201 gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca ggatccctac
     7261 cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat gcggagcatc
     7321 ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt gtttggcctg
     7381 ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat gcatatgaag
     7441 gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag caggaatcct
     7501 gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct cccctttttc
     7561 gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg taatcgcggc
     7621 gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag gcggtaaggg
     7681 ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca gcgccgcgag
     7741 caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg cgccatccgg
     7801 taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat tcggtaacaa
     7861 attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc cggcaaagct
     7921 ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa agcgccaggt
     7981 ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc cgatggtaaa
     8041 atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct ggatgcgata
     8101 atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca gcatattcct gcatttgctc
     8161 gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca tttcagggtg
     8221 tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca ccgctttaag
     8281 atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat cagcagagtc
     8341 attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc gcagtgcctc
     8401 aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac tgactcccga
     8461 aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc caccagcata
     8521 acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca cacattgatt
     8581 gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt catgatttcc
     8641 ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg tccgagtggc
     8701 tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgggggttcg aatccccccc
     8761 tcaccgccat atttaaagaa gagctcgtac gaaagtacgg gctttttttt cgtatattgc
     8821 acacaccggg gggatgagaa gccccgaccg gggttcgaca actggcgaca gccagttgga
     8881 cagaccgtga acgcagtgag cgggctgccc gcagggcgag cgaagcgagt caatcccccc
     8941 ctcaccgcca tctttaaaga agagctcgta cgaaggtacg ggcttttttt cgtatattgc
     9001 acacaccggg gggatgagaa gccccgaccg ggatacttag ttaatgcacc ggttcactct
     9061 ttgtcgccgc gactaatggg cgaattttgt cacgagtctc ccagaccgga ggaatttcct
     9121 ccaatttagt gacagaagct cgtacaaaag gcacccgtgc aagagtcata attgtatcca
     9181 accaacggtc acgaataatg cgatccgccc tttcatgact cgggtcatcc agctcaataa
     9241 cacagactat tttattgttc ttctttgaaa ccagaacata atccattgcc atgccattat
     9301 aaattgcaaa gaaacgcttt ctttcttctt cgctaaactc aggttgttct ggaaagttga
     9361 tcaagcgccc cagagagacc tggcaatgga tatgacaata atgtccaaac catttttcca
     9421 gattatcgaa atacattttt tcacaacgcg tgattaacca ctcttttctt tccaataaaa
     9481 taccttcggc aaccattccc tgaaataact catctcgctc ttgccaacga gagtcacgat
     9541 aggttttctg cggtatatag ctccttgtcg acgccttagc ggcaggggaa gttgcagatg
     9601 aagggacggg ttgcgattta gcgatgatta ccgatgaggc aacttttcgg agtgtcaatt
     9661 ttctggcaac agcaaaaccg gcgataaatg caattgtcaa acaagttaca aaagccataa
     9721 agctcataat taattttcac ctatccatgt tgattatatt tcattaccaa tgttactcgc
     9781 tgcagcgtgt tacatgccaa caatgcaacg atcaatatat agttagtatc atgttttgat
     9841 tattacaacc atccctattt agccattgcg gcttgccttc cacaaaaaaa taacaacaca
     9901 caatattagc aaagaattaa ttaagattaa ccttacattt ataatattat ccctaaagaa
     9961 taaatcagag gaatatattc ggtgttaaac agtcagtcaa agtatagccc attgttgatt
    10021 aaacctcata agcaaaattg gcaccagatc tcctgtacag gttgaatggc cgaaaaaaga
    10081 taggcagatg ctcatttttt tggcttctgg aagaaaaact ctccgcgctg gcagtattac
    10141 actggtctga tgctatgacg aaaaatgcct ggtaagagta tctaaaccag actccagttc
    10201 agcttatcaa cacgctcaaa gtcgcccccc tgaacgctaa cctgaagtga aaatctgttg
    10261 aaataaaaca aagcccggtt cgcccgggct ctgcaccgat aacacactta actgtaggca
    10321 tgcagcgtac gttggcaaag tgccgaacgt acgcagtcct ctttaccgaa ccggacgatc
    10381 ccaaccattt catcttcttc gaaacgttcc agcgcgtcac ttaatccgga gcacacgccg
    10441 cgaggcaaat cgcattgcgt gatatcaccg ttgacgataa ccgtcacgtt ctccccgagg
    10501 cgggttaaaa acattttcat ttgcgcggca gtcacattct gcgcctcgtc aagaatgacg
    10561 actgcatttt caaaggtacg tccacgcata taggcgaacg gcgcaatttc caccttccct
    10621 atttccggtc gcaggcagta ctgcataaag gaagccccta agcgccggac cagcacgtcg
    10681 tagaccgggc gaaaataggg agcaaacttt tctgcgatat ctccaggtaa gaagccaaga
    10741 tcttcatcgg cttgcagaac tggacgggtg acgataatcc tgtcgacatc cttatgtatc
    10801 agggcctctg ccgcttttgc tgcgctgatc caggtttttc cgcccccggc ttcgcccgtg
    10861 gcgaatatca gctgcttact ctcaatagcc ttcagatagt gcaattgcgc ttcatttcgc
    10921 gcgaggatgg gcgaagtatc gcgactgtcg cgggccatac caatggcttc tacgccgccc
    10981 atctgcacaa gcgaggtgac cgattcttct tcacgctgct tatggctgcg cgaatcccgt
    11041 ctcagcacac gttttgcctc gcgacgagct ttgatcactg ctttttgtct tcccatagag
    11101 agcaccttga gttgtttgta ttcatcacac gcgccgttgg cagcgcgatt atgcgcacga
    11161 acatcagagg gttggcttcc ttgtaagcca tagtttgctt ttggataaaa tgccgaaaaa
    11221 cggctacgcg caccgtttac ggcgtcggta acacatgaaa agaaaggatg aggttgaaaa
    11281 tgcaaagtga cgagatgact accggaggag aaaactccgc gagtggtggc gcgttgatta
    11341 tctaaaacat gtccagtaca ggacgttacc atccgcgatc tccatagtga ctgactatca
    11401 ctgccgggaa cttccgctgc tacttaataa gtacaacaga tctcgcattt attgcaacaa
    11461 tatatttact tatatttaac tataaaacac tatttcagtg acattagttt ctactggaaa
    11521 gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct ttcagcagga tttgcatcag
    11581 gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta tccagcctac caaacccgca
    11641 ctgattgtag gcatgataag acgcgcaagc gtcgcatcag gcatctgcgc acaaatgcct
    11701 tatccgccta aaaacattaa acccgcaata acgcgcttcc gagataatcg ttcgcgtcct
    11761 tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg
    11821 cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtagc
    11881 agacaaacag caaccccata tccagttgtc cggagttggt gacccccaac gaataactgt
    11941 agccgcgacg cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga
    12001 tatggctgtc cagcgcaatc accttccctt ccgggtcgct ggcgtaatca ggcacatcat
    12061 gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct
    12121 gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct
    12181 gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc cacaccactt
    12241 tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca
    12301 aattaatcgg cgtctctttg cctttactac gcgacacgtg atcggaaata aacccttccc
    12361 gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga
    12421 taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga cataacgccg
    12481 catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc atctgtggcg
    12541 caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat
    12601 cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg
    12661 tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca
    12721 accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg
    12781 cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc
    12841 gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac
    12901 aacttcccgc cagcgccagc gcaccaatcc ctttcagtaa acgtcggcgt gacggttcat
    12961 tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca caccgcgaag
    13021 ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc
    13081 ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc
    13141 gaccttttta aagttggcat cgacttttgc cagcagttcc gggttggctt tttgcagttg
    13201 tggacgcagc aaatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag
    13261 atcggtgtgg ctgtagcgat cttcttcacc gctaattttg ctggctgcca cttcctcaat
    13321 cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt
    13381 ttgcaaatcg accacatctg tataaagctg ctcagcgtac tgatccatcc ctttggtggt
    13441 gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg ggtcggcggc
    13501 tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc
    13561 agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt
    13621 ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca ccagctgcgt
    13681 ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc
    13741 atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt
    13801 agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg
    13861 gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc
    13921 ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc cggcgttaac
    13981 cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct gcggcacatc
    14041 agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact gcaatgcgtt
    14101 acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa
    14161 gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc gtggtggcag
    14221 agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct
    14281 cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga
    14341 gagcactgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg
    14401 aaatgcgcga atggcaccag cagccagccc tgcggcgacg aagagaataa acaggctggt
    14461 ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagcctag
    14521 caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg
    14581 ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa
    14641 gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc
    14701 ctgctccagt tgtactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt
    14761 aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgtt ctttttgcgg
    14821 aaattcgccg gtggtttcgt taatgaagat acccaagccc aggcacaacg cagcggcaag
    14881 caacacgcca atccacatca cgccaatcca tcggcctcgc tgggtacgct taagatagct
    14941 ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg cgcaacataa tgagaaacgg
    15001 aacaaacatg ccacccaccc ttaaacgttt ttctgagtca atccatgcaa agaaaagtaa
    15061 atcgcagtga tagcgattat cattacggca aagaaaaata caagcggaat gtttataaaa
    15121 tgatgttgga atgtaaacaa agagaggtaa taccagggga tataaacgat tattccaggt
    15181 acaaagatgg gattgtttac aacgagccat gtgttcttgc cagatgccgc gtaaacgcct
    15241 tatccagccg acaaaactgt ccagactcaa tgcatcacag cttccatgta aaactgataa
    15301 gcgggtgtat caagcaattt cgtccatccg cgattatcaa atctaacttt ttgcccggaa
    15361 cataataaca gcgcgggtgt tggtggcgtc aaaatcgctg aaacgttccc cgaaggccgg
    15421 ggcagcccac atggatgtgg gctgagggcg cgtcttacag gacgttacct cgcgcccgac
    15481 ccggtagccg aaggggataa gctgaaggta ccgcgcagcg gcgattttgt tcgccagagc
    15541 ccgggggtgc agggggcggc ggcgactggc cgccccctgc gcgctccctg caccattcaa
    15601 tttatatggc gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc
    15661 gcaggctaag tcccttagtt ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca
    15721 tcggcctcgg caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac
    15781 actacaatcc cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag
    15841 ccgaactgct tccagacgat aactgtcagc gcaccgatga tcatccccgc cagcgcaccg
    15901 ttgcgcgtca tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca
    15961 aagcctgccc acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc
    16021 gcaatcgcca ccagcgccac caccagcacc atcacacgtc ctacccacac cagctctttc
    16081 tggctggcct gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac
    16141 accagcagct ggcaacttaa ggttgacatt accgccgcca gaatcgccga cagcagaatc
    16201 ccggcaatcc acgggttaaa cagaatttgc gccagttcga taaacacgcg ttcagcgttc
    16261 tggtttaccg cacctgctac cgccggatgc tcgttaaagt aagcgatacc aaagaagcca
    16321 acagccaccg cccctgccag gcaaaggatc atccaggtca tactgatacg acgcgcatgg
    16381 acaatgctgt ggtgagaatc tgccgccata aaacgcgcca ggatgtgcgg ctgcccgaag
    16441 taacccagcc cccaacccat caatgagata atggcaacaa agttcagccc tttgagcata
    16501 tccacgtttt cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca
    16561 ctgataatga cgataaccgg cgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca
    16621 gtgtcagtcc agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgacgca
    16681 gctcccgcca acagagccgt ttcgtaactc atgccaaagg tactttcaaa cagacgcgcg
    16741 cctgccacaa tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag
    16801 ataatgcgca aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt
    16861 aaggcgttat tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg
    16921 atccacgcgc ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa
    16981 accgcgccag gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat
    17041 gccgtcacga atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt
    17101 gatcgccagg cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc
    17161 aacatcggtg tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa
    17221 acttgccgtt atatctgcca ccggaacggg gtaacagagt ttatgtttta ccagggcgac
    17281 cgtatcctgc cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat
    17341 tttagtgctc agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg
    17401 cacctttcaa aaaggttaac tgccgcagag aaaaagtctg agttattttt taatcactgt
    17461 catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc aagtcacatt
    17521 taacatggtt gcacaaagtt gcaacattat ggatatttta cgataacgtt aagttgcacc
    17581 tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac
    17641 gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacacc acactggtta
    17701 attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag
    17761 ctacctgcgc tgctttctgg cgcggccaat gagagcgatg aagcaccgac tccggcagag
    17821 gaaccacacc agccattcct cgactttgcc gagcaaatat tgccccagtc ggtttcccgc
    17881 gccgcgatca ccgcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa
    17941 caagcccgcc tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc
    18001 gataaactgc gtaatcaaaa aaatgccagt ggtcgtgcag gaatggtcca agggttattg
    18061 caggagtttt cgctgtcatc gcaggaaggc gtggcgctga tgtgtctggc agaagcgttg
    18121 ttgcgtattc ccgacaaagc cacccgcgac gcgttaattc gcgacaaaat cagcaacggt
    18181 aactggcagt cacacattgg tcgtagcccg tcactgtttg ttaatgccgc tacctggggg
    18241 ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg
    18301 aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg
    18361 cgcctgatgg gcgagcagtt cgtcacgggt gaaaccatcg cggaagcgtt agccaatgcc
    18421 cgcaagctgg aagagaaagg tttccgttac tcttacgata tgctgggcga agctgcactg
    18481 actgccgcag acgcacaggc gtatatggtt tcctatcagc aggcgattca cgccattggt
    18541 aaagcgtcta acggtcgtgg catctatgaa ggtccgggca tttcaatcaa actgtcggcg
    18601 ctgcatccgc gctacagccg cgcccagtat gaccgggtaa tggaagagct ttacccgcgt
    18661 ctgaaatcac tcaccctgct ggcgcgtcag tatgatattg gtattaacat cgatgccgaa
    18721 gaggccgatc gtctggagat ctccctcgat ctgctggaaa atctctgttt cgagccggaa
    18781 ctggcaggct ggaacggcat cggttttgtt attcaggctt atcaaaaacg ctgcccgttg
    18841 gtgatcgatt acctggttga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg
    18901 gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc
    18961 tatccggttt atacccgcaa ggtgtatacc gacgtttctt atctcgcctg tgcgaaaaag
    19021 ctgctggcgg tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg
    19081 gcagcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc
    19141 ctgcatggta tgggcgagcc actgtacgag caggtcaccg ggaaagttgc cgacggtaaa
    19201 cttaaccgac cgtgtcgtat ttatgccccg gttggcacac atgaaacgct gctggcgtat
    19261 ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac
    19321 acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg
    19381 caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac
    19441 ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg tctggcctcg
    19501 ctctcctctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa
    19561 caaccggtag cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt
    19621 gtgggctttg tgcgtgaagc cacgccacgt gaagtagaac aggcgctgga aagtgcggtt
    19681 aataacgcgc caatctggtt tgccacgcct ccggctgaac gcgcggcgat tttgcatcgc
    19741 gctgccgtgc taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc
    19801 ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac
    19861 tacgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg
    19921 gtgtgtatca gtccgtggaa cttcccgctg gctattttca ccgggcagat cgctgccgca
    19981 ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg
    20041 caagggatcg ccattttgct ggaagcgggt gtaccccctg gcgtggtgca attgctgcca
    20101 ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtgcg cggggtgatg
    20161 tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac
    20221 gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc
    20281 gattcttcag cactgaccga acaagtcgtc gtggatgtgc tggcctcggc gttcgacagt
    20341 gcgggtcagc gttgttcggc actgcgcgtg ctgtgcctgc aagatgagat tgccgaccac
    20401 acgttgaaaa tgctgcgcgg tgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg
    20461 accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt
    20521 cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat
    20581 gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt
    20641 gccgaattgc acaaagaggt ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac
    20701 cagctaccag agctgatcga gcagattaac gcttccggct atggtctgac gcttggtgtc
    20761 catacgcgta ttgatgaaac catcgcccag gtcactggct cggcccatgt tggtaacctg
    20821 tatgttaacc gtaatatggt gggcgcagtg gttggtgtgc agccgttcgg cggcgaaggg
    20881 ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat
    20941 cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat
    21001 gcacagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat
    21061 cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa
    21121 cgattgctgc cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc
    21181 gtgttgtgta ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg
    21241 gtgggcagcc aggtactgtg gccggatgac gcgctgcatc gtcagctagt aaaggcattg
    21301 ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccc
    21361 tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt
    21421 gctgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc
    21481 cttctggaac ggctgtatat cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt
    21541 aacgccagtt taatgacgat cggttaaaca ggtagccgga ggatgttaca acctcctccg
    21601 gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg
    21661 attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa
    21721 atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg
    21781 cgcgagtttg ccgcttttga cccaaccggc aatcagcgca gatttctcat caattaatgc
    21841 cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca tctccataca
    21901 gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc agcttcagac ggatgtactc
    21961 ttttatcgcc gccagcggag cgaaatcttc acgaaacgct tttaacggtg ccagccagat
    22021 atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag
    22081 cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa
    22141 accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac
    22201 tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt
    22261 gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc actctcatcg
    22321 cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt tttatcag
//
LOCUS       DAZRVY010000060        22239 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZRVY010000060 DAZRVY010000000
VERSION     DAZRVY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.060"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXOX_002377"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXOX_002377"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR7005821.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHXOX_002378"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHXOX_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR7005822.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHXOX_002379"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHXOX_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR7005823.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHXOX_002380"
     CDS             2284..2730
                     /locus_tag="ACHXOX_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR7005824.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHXOX_002381"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHXOX_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR7005825.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHXOX_002382"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHXOX_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR7005826.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHXOX_002383"
     CDS             4841..5188
                     /locus_tag="ACHXOX_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR7005827.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHXOX_002384"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHXOX_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR7005828.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHXOX_002385"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHXOX_002385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR7005829.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="ACHXOX_002386"
     CDS             6705..6803
                     /locus_tag="ACHXOX_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR7005830.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHXOX_002387"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHXOX_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR7005831.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="ACHXOX_002388"
     CDS             complement(6939..7187)
                     /locus_tag="ACHXOX_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR7005832.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="ACHXOX_002389"
     CDS             complement(7335..7517)
                     /locus_tag="ACHXOX_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR7005833.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHXOX_002390"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHXOX_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR7005834.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHXOX_002391"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHXOX_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR7005835.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHXOX_002392"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHXOX_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR7005836.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHXOX_002393"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHXOX_002393"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR7005837.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHXOX_002394"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHXOX_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR7005838.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHXOX_002395"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHXOX_002395"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR7005839.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHXOX_002396"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHXOX_002396"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR7005840.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHXOX_002397"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHXOX_002397"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR7005841.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHXOX_002398"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHXOX_002398"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR7005842.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHXOX_002399"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHXOX_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR7005843.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHXOX_002400"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHXOX_002400"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR7005844.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="ACHXOX_002401"
     CDS             complement(19482..21392)
                     /locus_tag="ACHXOX_002401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR7005845.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="ACHXOX_002402"
     CDS             21524..21868
                     /locus_tag="ACHXOX_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR7005846.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5450 a         5698 c         5400 g         5691 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacacctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga acaatctggt tatagcgtgc ggactgaccg ttaattttct ccttcttact
    22141 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    22201 ccatatcgtt ataatccctt tgatattaga tgcaaatta
//
LOCUS       DAZRVY010000061        21965 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZRVY010000061 DAZRVY010000000
VERSION     DAZRVY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.061"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..323)
                     /locus_tag="ACHXOX_002403"
     CDS             complement(<1..323)
                     /locus_tag="ACHXOX_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR7005847.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIR"
     gene            complement(525..1169)
                     /gene="cheZ"
                     /locus_tag="ACHXOX_002404"
     CDS             complement(525..1169)
                     /gene="cheZ"
                     /locus_tag="ACHXOX_002404"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR7005848.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1180..1569)
                     /gene="cheY"
                     /locus_tag="ACHXOX_002405"
     CDS             complement(1180..1569)
                     /gene="cheY"
                     /locus_tag="ACHXOX_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR7005849.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1584..2633)
                     /gene="cheB"
                     /locus_tag="ACHXOX_002406"
     CDS             complement(1584..2633)
                     /gene="cheB"
                     /locus_tag="ACHXOX_002406"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR7005850.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2636..3496)
                     /gene="cheR"
                     /locus_tag="ACHXOX_002407"
     CDS             complement(2636..3496)
                     /gene="cheR"
                     /locus_tag="ACHXOX_002407"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR7005851.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3515..5116)
                     /gene="tap"
                     /locus_tag="ACHXOX_002408"
     CDS             complement(3515..5116)
                     /gene="tap"
                     /locus_tag="ACHXOX_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR7005852.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5162..6823)
                     /gene="tar"
                     /locus_tag="ACHXOX_002409"
     CDS             complement(5162..6823)
                     /gene="tar"
                     /locus_tag="ACHXOX_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR7005853.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6968..7471)
                     /gene="cheW"
                     /locus_tag="ACHXOX_002410"
     CDS             complement(6968..7471)
                     /gene="cheW"
                     /locus_tag="ACHXOX_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR7005854.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7492..9456)
                     /gene="cheA"
                     /locus_tag="ACHXOX_002411"
     CDS             complement(7492..9456)
                     /gene="cheA"
                     /locus_tag="ACHXOX_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR7005855.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9461..10387)
                     /gene="motB"
                     /locus_tag="ACHXOX_002412"
     CDS             complement(9461..10387)
                     /gene="motB"
                     /locus_tag="ACHXOX_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR7005856.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10384..11271)
                     /gene="motA"
                     /locus_tag="ACHXOX_002413"
     CDS             complement(10384..11271)
                     /gene="motA"
                     /locus_tag="ACHXOX_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR7005857.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11397..11975)
                     /gene="flhC"
                     /locus_tag="ACHXOX_002414"
     CDS             complement(11397..11975)
                     /gene="flhC"
                     /locus_tag="ACHXOX_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR7005858.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11978..12328)
                     /gene="flhD"
                     /locus_tag="ACHXOX_002415"
     CDS             complement(11978..12328)
                     /gene="flhD"
                     /locus_tag="ACHXOX_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR7005859.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13108..13536
                     /gene="uspC"
                     /locus_tag="ACHXOX_002416"
     CDS             13108..13536
                     /gene="uspC"
                     /locus_tag="ACHXOX_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR7005860.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13543..14967)
                     /gene="otsA"
                     /locus_tag="ACHXOX_002417"
     CDS             complement(13543..14967)
                     /gene="otsA"
                     /locus_tag="ACHXOX_002417"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR7005861.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14942..15742)
                     /gene="otsB"
                     /locus_tag="ACHXOX_002418"
     CDS             complement(14942..15742)
                     /gene="otsB"
                     /locus_tag="ACHXOX_002418"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR7005862.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15909..16895)
                     /gene="araH"
                     /locus_tag="ACHXOX_002419"
     CDS             complement(15909..16895)
                     /gene="araH"
                     /locus_tag="ACHXOX_002419"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR7005863.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16910..18424)
                     /gene="araG"
                     /locus_tag="ACHXOX_002420"
     CDS             complement(16910..18424)
                     /gene="araG"
                     /locus_tag="ACHXOX_002420"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR7005864.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18494..19483)
                     /gene="araF"
                     /locus_tag="ACHXOX_002421"
     CDS             complement(18494..19483)
                     /gene="araF"
                     /locus_tag="ACHXOX_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR7005865.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            20280..20783
                     /locus_tag="ACHXOX_002422"
     CDS             20280..20783
                     /locus_tag="ACHXOX_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR7005866.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20862..21113)
                     /gene="yecJ"
                     /locus_tag="ACHXOX_002423"
     CDS             complement(20862..21113)
                     /gene="yecJ"
                     /locus_tag="ACHXOX_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR7005867.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(21228..21314)
                     /gene="azuC"
                     /locus_tag="ACHXOX_002424"
     CDS             complement(21228..21314)
                     /gene="azuC"
                     /locus_tag="ACHXOX_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR7005868.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            21577..21900
                     /locus_tag="ACHXOX_002425"
     CDS             21577..21900
                     /locus_tag="ACHXOX_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR7005869.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         5369 a         5839 c         5172 g         5585 t
ORIGIN      
        1 cgaattacat aggggctcgc agcgttacgg ctgcaataaa gccggatata aagctgcaac
       61 ctacccgtca tgtcaaaaca atggatgcct tgcaaacggg atgcgttcat ataaatactg
      121 tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg
      181 ccaaaccaga taacacacac gcccactaac aaaatcagca acgaggtcag ttcacgggaa
      241 cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt
      301 ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cttaaatgat
      361 gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct
      421 tttgctgcca cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca
      481 gaccgcctga tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta
      541 tccaacaaat cgtccacctg atcctgactg gctaccacgc cgactttgct ggtatcgacc
      601 tgaggtccat taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg
      661 ttttccaaca gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc
      721 ttaatgacca gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc
      781 gcgttagtaa agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc
      841 agttcacggg catcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt
      901 aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc
      961 agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca
     1021 tcggggatgg cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg
     1081 tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg
     1141 tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa
     1201 gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact
     1261 ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa
     1321 cactggcaat gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc
     1381 catattgggc atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt
     1441 gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct ctttcagcag
     1501 gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt
     1561 atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc
     1621 aacatttgct ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg
     1681 gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc
     1741 cccgcctgac gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc
     1801 accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa
     1861 ggccgatgac ggttaaccgc cgggccgtcg tgaattttga tttgataatt tgcgccacta
     1921 cgcgccagtt ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt
     1981 tctccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa
     2041 gagcgggtga aaccgggcgg catatgttgg gtaattaaca atgccgggct ggaaagcggc
     2101 aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc
     2161 agtttttcag aactcaacaa cggacccgcc ttcagcgttg tcggtgctga caatggctta
     2221 tgtgctgcaa ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgctatac
     2281 gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc
     2341 tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca aggaagaaac catcacaacg
     2401 ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt
     2461 tcaacatcca gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc
     2521 ggatcaggcg cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg
     2581 atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat
     2641 ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa
     2701 agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc
     2761 gcaaaatctc ctgctgggta ttttgatcga agtagatcat gacgttacga cagaagatcg
     2821 catcaaacgg ccccggcacg gtgtactgtt tcgccagtag attcagcggg gcaaaatcaa
     2881 catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca
     2941 tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac
     3001 cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc
     3061 cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg
     3121 tcgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga
     3181 gcagagggaa atggtgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga
     3241 taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga
     3301 aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt
     3361 tatggtcagc cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat
     3421 gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg
     3481 gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta
     3541 actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg
     3601 acgaaagatg gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca
     3661 gcgaggcgtt ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa
     3721 tcccccgctg ttgttcttcc gacgcagagg caatttctcc cataatgtcg ttcacgcggg
     3781 tcaccgaact gacaatatcg atcatggtcg cggcggcgtt attcaccagt ttcgaaccct
     3841 gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc
     3901 tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc
     3961 cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc
     4021 cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg
     4081 tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg
     4141 ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct
     4201 ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat
     4261 cgttattgcc tgcgacaatc tccgcgatac caatgtgcat ctcctggctt cccttacgca
     4321 catcacttac cgtcccacgc aaagcctgct gcatggtctt cagactggca aaaatggcgg
     4381 tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt
     4441 caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg
     4501 cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagt gtcgaaatct
     4561 gatagttacg ctggctttgc gcactggcgg cttccagcac atggttgatc tccagctgcc
     4621 aggcctcaaa gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat
     4681 ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg
     4741 caaaactttt ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg
     4801 ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca
     4861 acgttttaat gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg
     4921 cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc
     4981 tctgctcgac ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt
     5041 tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc
     5101 gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc
     5161 gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgccggtg
     5221 gttgctcact ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca
     5281 gacggaacgc ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg
     5341 cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa
     5401 ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc
     5461 ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc
     5521 tttcgaccag caccgaaccg gtatcaacgc gtgcgacgga gtcttcaatg agggctttga
     5581 tctcttttgc cgcctgggcg ctgcgactgg caagattacg cacttcaccc gccaccacgg
     5641 caaaaccacg gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga
     5701 tattagtctg gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac
     5761 tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg
     5821 cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct
     5881 tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct
     5941 gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga
     6001 tggcatctga accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg
     6061 aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcta
     6121 ggttaccacc ggcgatttcg cgaatgtgag caataatttt tgccagcgga gtaagcaaca
     6181 tacggcgaat gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg
     6241 ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca
     6301 gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct
     6361 gggttggctg agcgaaataa gctccagtat tgccataatc tagataatca atcagttcag
     6421 ttaacgctgt gtaatagttt ttatattttt catcaatatt acgactggta gcgaccattt
     6481 caggtaacgg tgccatgctt ttgaattttt tataatgcgt cgctgcctgc gccaatgttt
     6541 tcctggcgct atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca
     6601 tcatccgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg
     6661 acgtcagctc gccctgctgt tcccgcaatt gattggaaac cacaaagctc ttctggctat
     6721 ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca
     6781 gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa
     6841 acaagttgat ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg
     6901 cgaaattgaa ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc
     6961 gggagaatta cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca
     7021 gcagtttttc gatgttcacc agaatcaaca tacggtcgcc cagtgcgccc agtccagtga
     7081 gatattctgt tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca
     7141 atgaaagcac gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca
     7201 ggacgataac taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta
     7261 agtcaacaat cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg
     7321 tgttcgcaat ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa
     7381 taccgtactc ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg
     7441 ccagctttgt tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc
     7501 ggtgttcgcc atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag
     7561 tgccacgctg ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt
     7621 actttcaagg tttttaacca caacctggtg ttgaccaatt aattgatcca ccagcaaggc
     7681 gtagcggcga ccgccacttt gtaagatcac aacaattccc tgggtggctt cggttttcgc
     7741 gcccgcgacg ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc
     7801 cagcacccgc tcgccgccgg ccagtggatg gagatcggct tcacggggtt gcagtgattc
     7861 cataacagca ttcagcggca gaatgaaaac ttcatcccca acgcgtacgg acatgccgtc
     7921 gaggatggcc agcgtcagcg gcagtaaaat gcggatcgta gtgccagtcc cctgcttcga
     7981 ctggatttcg acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac
     8041 gccgcgcccg gagacgtcgg tgacctgctc tgccatggag aagcctggtg caaatatcag
     8101 catcgcgact tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt
     8161 tgccagaatt cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt
     8221 gccgccctga tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag
     8281 ccgtttttct ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg
     8341 gtcgataatg cgttctatca ggcttttgtc gagttcagta gaactgccca ccagcgtcag
     8401 ttctacctgc ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa
     8461 aacatattcc atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt
     8521 acgttgtaac tgccccatgc tggttatcaa atcaccatga ttaaccgggt ccagttcgct
     8581 ggaacgctgg gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg
     8641 atcaaccttt tctaccgcta cacggatgct ggtggattca ttgctgcgcg tcgttttttc
     8701 ccgctccacg cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggtgga
     8761 tatttttggc gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca
     8821 gagtaccgct gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc
     8881 cgcccctttc accacgtcag ttaacgttgt cagatgtccc agttcttctt ccagcaggtc
     8941 gacttccccg gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg
     9001 ctcatcttgc ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc
     9061 gcctttcgct tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc
     9121 atccggctct tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc
     9181 caaaaacaga ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag
     9241 caggttttcc atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc
     9301 tcctttgatc gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc
     9361 cggctgcaaa accagcagat gctgctccat gtcagccaac agttcgtccg cttcatcaaa
     9421 aaatgtctga taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg
     9481 gcattgtggg aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct
     9541 cattctggct ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca
     9601 gcaggctgat gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg
     9661 ccgccatgcc gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc
     9721 ggcgggatgc attggcccga tcggcagaaa gctcccagtt gctatatcct ttctcaccgc
     9781 tggcgtaggg gaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca
     9841 gtacaggcgc aatggcacgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg
     9901 ttctaaacat cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca
     9961 gatcgatttt gagatgggga cgtaacgccc gcagtttcgg atcggactct atcaactggt
    10021 cgagatcacc ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agatcttcga
    10081 tgttcggctg cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa
    10141 ttgggctttc actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc agtggagtcc
    10201 ggaagtactc cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca
    10261 ccagaaaaaa ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat
    10321 gtgctgcccc gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat
    10381 tcttcatgct tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc
    10441 ttccagttca ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac
    10501 ggcgataggc ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat
    10561 cattttgctg gtttcggcgc ttttctgacg taaaacactc gctaatgggg aaataaatcc
    10621 gtaagccaat aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag
    10681 ctcggcggca ggacgatcgg ctgaacctaa cgcgtgaacg ccccccatta cagccgcgac
    10741 aataccaaac gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc
    10801 ctcgctttcg tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat
    10861 atgaccgctg ataatcagac gcagataatc gacgataaaa tcaagcatga cgctatccgc
    10921 gaggatgcgt gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc
    10981 cagcgaaaac atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag
    11041 atccatatac attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag
    11101 cgtgccttta atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc
    11161 aataatcacc agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata
    11221 accgccgaaa actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc
    11281 cttccactgt tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca
    11341 aaaaaagcag cggtacgtcg ttaccgctgc tggaatgttg cgcctcaccg tatcagttaa
    11401 acagcctgta ctctctgttc atccagcagt tgtgggataa tatcggcagg attctgggaa
    11461 agtttacgtc tttttactgc ccgggatggc ggttgacata agctgcaggc aaagctgcca
    11521 acaggctggt gagcgtgggt aataaaattg ccgccgcagc agttgcagct ggaaagttgc
    11581 agtaatccac tttcaacaaa ccgcaccaat gtccaggcac gggttaatgc cagcagtggt
    11641 ccttcttctg cttgtgggca ctgttcaagg tataaacggt aggctttgat caccgcatcg
    11701 acgccattac acaaaccggt tttcagtaaa aactgccatg cattacagaa catcgaagca
    11761 tgaacgtttt gttcccaggt cataaaccag tcggttgaga atggcagcat gcctttcggc
    11821 ggtgggcttc cgcgcagttc tttataaagt tttatcaggc gtccgcgact taactgtgtt
    11881 tcgctttcca gcatctgcaa acgagcgccc agggtaatca attccattgc cagctgaata
    11941 tcccgcgctt cctgaacaat gcttttttca ctcatgatca ggcccttttc ttgcgcagcg
    12001 cttcttcagg ctgattaaca tcattcagca agcgtgttga gagcatgatg ccggtatgaa
    12061 tttgctggag atcgtcaacg cgggaatctt gcgtcaactg agtaatcgtc tggtggctgt
    12121 caaaacggaa gtgacaaacc agttgattgg tttctgccag cttaaccatt tgcggaagag
    12181 tcagtgccgc taacgttgtc gccatttctt catttatgcc gagacgaaac atagcggacg
    12241 ctttgtcctg aacaatcaaa cgctgtgcaa gtagtaaata tgacaagttg atgtcataaa
    12301 tgtgtttcag caactcggag gtatgcatta ttcccaccca gaataaccaa ctttattttt
    12361 atgcggtttc accgcacccc gtgatgtcgc cgggaagccc cggtaaaaaa taattagcat
    12421 tagaatagtt gcgataagct gcaataagca gaaccacctt tttggtttaa tatgtcctta
    12481 caaatagaaa tgggtcttta cacttatcta agatttttcc taaatcgacg caactgtact
    12541 cgtcactaca cgcacataca acggaggggg gctgcgattt tcaataatgt gtgatgcaga
    12601 tcacacaaaa cactcaatta cttaacataa atgtataagt catacttttg ttttgggtgt
    12661 atttcaatat gttaaaaagt ttttcgctac gctagcaagc aaaaatgaaa caggaataat
    12721 cgaaatggga tgttgcgcac agttaaaata aatcaccgta aataatcatc tgctataaat
    12781 aatcactttc atgcaatacc agataagcta tttttaaaca gacacttacc gcacaacaaa
    12841 ctaattaaat acaaagaaag gcaacaatca acttaatgtt aatgaaatgt tttacatata
    12901 ttaaccactt gtttaatgcg gagaatcagt acactatttt cttataatta catttgaaat
    12961 tatatgatca ccgggagtga ttgattaatt gtctttacta ataatcagac taatatttac
    13021 ctgtttgacc gagttgggat tgcgtcgttt ctccattagg agtaaagctt taatgtcacc
    13081 tgaagttcac agaataagga acaggttatg agctatagca atattcttgt cgctgttgcg
    13141 gttacaccgg aaagtcagca actgctggca aaagcggtat ctatcgccag gccagtaaag
    13201 ggacacatca gtttaattac tctcgcttcc gacccggaaa tgtacaatca attagccgcg
    13261 ccgatgcttg aagatttacg cagcgtgatg caagaagaaa cgcaaagctt tcttgataag
    13321 ttaattcagg atgcggggta tcccgttgac aagacgttta ttgcctacgg tgagttaagc
    13381 gaacatattc tggaagtatg tcacaaacat catttcgatt tggtgatttg cggtaatcac
    13441 aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa gagtgattgc ctcaagtgag
    13501 gtcgatgtgc tgttagttcc acttacggga gattaaccgc tcctacgcaa gctttggaaa
    13561 ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc ggaactatct gctttaggtc
    13621 gctaatgaag cactcctgcc agtggttaat atcgtttttc acgataacgt ccagcatttc
    13681 tgcatgacgg gaaatacgtt ccgccagcga catagtcaat gcacgatcca gcgcagctgc
    13741 aacttcgtca cgatcgtagg ggttaacaat taacgccgac gttaactcgt ttgccgctcc
    13801 cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg tcctgagcag caacatactc
    13861 ttttgctacc aggttcatcc cgtcacgcag tggcgtcact aagcccacgt cagagtagcg
    13921 gaatattttc atcagtaatt tacggtcaaa atgctgattc aaataataaa gcggcgtcca
    13981 gcctaattgc ccgtatttac cattaattcg tccagcttca ttttcgagct gatgacgaat
    14041 atcctgatag gcttgcacat caccacgcga cgttggtgca atctgggtat aacgaatttt
    14101 accatgatgc tgcggatatt tttccagcaa cgcttcatag gcgagaaaac gctctggcaa
    14161 acctttggaa taatccagcc gttcgacaga aaagatattt tgtacgtttt tcagttccgc
    14221 tttaagttgc gccagttttg gcggcagtgg cccggcagcc tgtttggcta tttctttcgg
    14281 ttcaatgccg atcgggtaga cttctgttcg aaatgctttg ccccaggctg tatggctttt
    14341 tgcgctacgt gtcgtgacgc gggtcaggtt agaaagacaa tccaggaacg ccagacgatc
    14401 gttttctgtc tggaaaccca gcaaatcata atcacaaagc tgttcaagca aggtgtcata
    14461 tgtcggcagc gcgttgaaga tttccggtgt cgggaaagga atatgcagaa agaaaccaat
    14521 gcgattattc actccccgtt tgcgtaattc atgcgcaaat ggcaacaggt gataatcgtg
    14581 gatccagata atgtcatcgt cttgcaacag cggcagtaat ttatctgcca gcaacgcatt
    14641 tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc accagatcga gccgataatg
    14701 aaaagcgggc cagagaacgg cattggagaa ttggttgtag tattcgtcaa ggtcctgttc
    14761 gctgaggtta aaagaggccc acgtaatgtt accttttttc acctttttta gcggctgatc
    14821 ctcattccct gtttcaccac tccagccaaa ccacagtccg cctgcggctt tcagtgcccc
    14881 cagtatgcca acggcaaggc caccggcact ggcggcgtgc tcgtctggtg gtgcaatccg
    14941 gttagatact acgactaaac gactcatagt catcactcct gttattttct cttttttgtt
    15001 gtaatgcggt ggttatcatt tcaagccagc tccagacatc cggcacaccc gccagtcgcc
    15061 atgatgcctg agttgcacct gtgccaattt ttactgacat tccgcccagt cggttaacga
    15121 ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa tacgggcgtt cgcccgataa
    15181 agggagcttc ctgcataaaa gctgcaattg cctcaccttt actggtacct ctcggtttga
    15241 tctcgacaac acactttccc tgctgtaacg ccatttgtgg ccagatctga gtaatacgtt
    15301 gcgctaatgt cattaatgcg tcttcatgct gcggagcctg acgataatgc agcgcaaaag
    15361 ccatcccttt cgcctccagc tccgcgccgg gatactgagc gatgactgta tgcagttgca
    15421 cgctaatatc acgcgcaatc gcatccggca gatgaacgat atgtgtttta ccattgatgt
    15481 cacggcgctc cgccccatgc acgcccgcta acgggaagcg ataaggtttt gccagtgcgt
    15541 caagctccac cattgagcgc cctgatatca atgccaatgc accatcactt gcggttgcca
    15601 gtagctgtag tccttgcaga atattgtcag gcacgacgac ctgatcggga tgcggtttga
    15661 tttccgccag cgttccatca agatcaaaaa accaggcata tttcgcggat agttcagggg
    15721 tttcggttaa cggttctgtc acccggctct cctccttctc ttttcttatt gttttgcgca
    15781 gaacgcgctt tacagacatg taagtataga cagtgtgacg ggggtcgcca tttgcaatga
    15841 agaacagcca accgttgtgg cggctggctg tggtgggaaa aaacgttaaa ttgttgtgga
    15901 aaaaagcatc agacagtgcg tttcgctttt tgcttgtaac ggtcgaagat caccgctgcc
    15961 agcaggatta agccgcgaac cacgtactgc gcgaaaggag aaatattaag caggttcatg
    16021 gcgttttcca cggtgcctaa aattaagata cccgccacca catatgagat ttttccgatg
    16081 ccacctttca gagaaacgcc acctaaaacg caggcggaga taacaataag ctcataacca
    16141 atcgacgtca ttggctgccc actagtcata cgtgaagcca gaataattcc ggctatcgca
    16201 gataccaggc cagagagaac aaagataata attttggtgc gaacaaccgg tacacccgcc
    16261 agacgcgcag cctcttcgtt cccgccaatc gccagggtgt tacgaccaaa ggtggtttta
    16321 ttcagcaaca aaccaaagat aatcagacac gcgacggtga gccagattgg cgcaggcaga
    16381 ccgaaccagt tagcgtaacc aagggcaaag aagctttcat cttcgatacc gaccgcttta
    16441 ccgtctgaaa tgatgtacgc cagacctcga acaatctgca tcgttgccag tgttgtgatc
    16501 agagcattta ttttcagttt ggcgataaca aagccattga ccaggccaca gagaatgcca
    16561 agcagcaacc ccgctgccac gccaatccac aggctttcag tcaggttgat aaccaccgcc
    16621 gtggtgacac ccgcacaggc aattacggag gcgacagaaa ggtcaaagtc accggaagca
    16681 aggcagaaca acatgccaca cgccaccatc cccgacatgg aaattgccag gcccaaccct
    16741 ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg cacaggcgat aaagagcacc
    16801 gcaaacacca ccagcatgcc gtactgatcc caaatacgcc cgaagctgaa tgacgactta
    16861 ggtgcgccag aacccgatgt agaaacagaa gacatcatac tctccttact caggcaactg
    16921 cctggctgac tttaggcatc gcaaggctca gtgcctgacg ctcatctgcc tgctcgtgta
    16981 acaattcacc ggcgatttca ccttcccgca tcaccacaat ccggtcggca acgccgagga
    17041 cttcaggtaa gtcgctggag gcaaacagca ccgccacacc ctgcgccgcc agcgcataaa
    17101 ttacgttata tatttcgtgc ttagcaccaa catcaatgct gcgcgtaggt tcatccagca
    17161 aaatgacctt catctcttcc gataaccagc ggcccagaat ggctttttgc tgatttccgc
    17221 ctgagagatt catgatcagt tgctcagcgc ccggcgtttt gatgttgagc gaacgaatgt
    17281 gctgatcggc attgttttct tcccaaccgt tgttgattac acaaccgccg agcacatgtt
    17341 tgcgtctggc actgatgttg atattgtcgc gaacggagtg cacgggaata atgccttccg
    17401 ctttgcgatc ttccgggcag agcatcatgc ctgcggcaat ggcgtggctc ggtttacgaa
    17461 tatcgatcgg ctgttggtcg atataaacct gaccggcggt gatttgcgtc ccgccaaaca
    17521 agcctttcat taattcgcta cgccccgccc ctaccagacc aaacagccca acaatttcac
    17581 cactgcgaac cgccagactt attggcgtac gcacgcctgg tgctttcaca gcatcaagac
    17641 gcaggcgctc ctcgccataa ctacgaggtt gccagccgta gatatcgcca atgtcgcgcc
    17701 cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg catatcggta aaggttttga
    17761 cataacgtcc atctttaaag acagtgatgg catcgctgag ggcaaatatt tcttccatac
    17821 ggtgagaaac gtataagatc acccgcccct cttttcgcag ttcacgaata acgcggaaaa
    17881 gattgtcgat ttcgcgggca gaaagggagc tggttggctc atcaaaggcg ataattttag
    17941 cgttacgcgc cagcgccttg gcgatttcaa ccatctgcca ctgaccaatg gagagatatt
    18001 tcagcggcgt gtccgggtca atatccatac caagatgttt aagttgtaaa cccgcctcat
    18061 aattcagcaa tgagcgattc acaatgccgc ctttatgcgg cagctggccg agatagatgt
    18121 tttccgcgac ggtcatttcc ggcacgagat gcagttcctg gtaaataatc gccacacccg
    18181 cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg cccattaatc actacagaac
    18241 ccgtggttgg cgcatagttg ccgctgagga tttttaagag agttgatttt cctgcgccat
    18301 tttcacccat caacgcatga acctgaccgg catagcagtc aaaactaata tccgtcagcg
    18361 ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga gagatacggg gtagactgtt
    18421 gcataacgtc tccgtgaatc actggtcgtt cacaccccct ctgcatcatg cagaggggaa
    18481 tttttccggc aaattactta ccgcctaaac cttttttctc cagttcttct ttaaagttgt
    18541 cacgcgtgat cagcaccacg tcggtaactt cggtaaattt tggcggttca acgtcttttg
    18601 ctacccagtt gtaaagcatt tcgctggatt tatagccatg tacgtccggg cttggcagca
    18661 gggaaccgta gaagccggtt gcctgtgctt tagacagttc gctcaacgca tccacaccgt
    18721 taatgccaat gccgatgata tcggcagctt taaagccctg accttccgtc gcgcgtacgc
    18781 cgcccagcac ggtgctgtcg ttcataccga cgatcagcca atgtttaact tccggatgtt
    18841 gaaccagcat tgagttggca gcgtcaaatg cccccgggat gtcgttagat ttggtaggta
    18901 cctgataaat ttgtttttcc gggaatccgg ccgctttcag cgcatccata gaacccgttg
    18961 tacggcgacg tgccgtatcc agttcgttgg cggtaatcgc catcaccgcg ctttctttga
    19021 catcccagcc acgtttctgc atctctttat acagttcctg accctgacgt tcgccaattt
    19081 tagtcgccgc catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa
    19141 actggtcatc cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag
    19201 agccgagttt ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc
    19261 tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc
    19321 ctaaatcctt cccggcttta tcggcaaact tccattcggt ctggaaccac ggctcttccg
    19381 gttgcttcac cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg
    19441 ctgccagacc aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta
    19501 gtgtcgtttt gtgtagggca aaaacgaatg actttcgtta aattaatcgg aaaacaaagc
    19561 attacctttt atctaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt
    19621 ctccatagga gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg
    19681 ggaaaagaca taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa
    19741 tccataagat tagcctggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt
    19801 ttaaccagtc aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc
    19861 aactcaaaga tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat
    19921 taattaacag aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa
    19981 atcatcactt catcatgaat tcaaattcat tgattaatat caacaagata caaaaagcac
    20041 tatcattaaa attcattgca gttacattga tttcatcaat gaaatgtaaa aatatataaa
    20101 cttgatgatt taagcatttt cttatacccg ttcagacgtt attcttattt cagatcatcg
    20161 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
    20221 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
    20281 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
    20341 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
    20401 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
    20461 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttcccggt gaaaaactga
    20521 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tctagtacgt
    20581 tggcacagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
    20641 gcgatctgga aaaagaacag cagcatgatg gtctgttgct gcaaaccatt cttgatgaag
    20701 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
    20761 tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat gcattgcgcc gatgaaggtt
    20821 ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga ttcaccctgt
    20881 ggggtaaact tgagttcaat aagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
    20941 tctgcggcgg cgggatggtt agtaaagtcg atattcttca gctgactgga cattttttca
    21001 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
    21061 caagcaacca catcagaaac cagttcctga tcggcattca gcggctggga catcgtaaac
    21121 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc gatagtcaca
    21181 tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
    21241 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
    21301 ttgcgcagtt tcatcgcggc actccgacca tttgttatac aggtgttatt gtctttgcgc
    21361 ttataatatg acaaccatca caaaaatcaa tctttatgtg atacaaatca cataaatacc
    21421 cctttaatgt tataaaaatg ataatcaaaa aacagccccc ctatttctga cacctacaga
    21481 tggcaagaaa tagcgcctgc caggcgtctt ttccggccat tgtcgcagcg ctgtaacgcg
    21541 taaaatagtg ctttctctta ctcttctggc tggaccatga gacttctgat tctgactctt
    21601 tcattaataa cgctggcggg gtgtacggtt actcgccaag cccatgtgag cgaagttgac
    21661 gcggcaacag gtattgtacg gttggtttat gaccaggctt ttttgcagca tgcccatacc
    21721 gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggaagg ctatacccac
    21781 gcggtcccct ttggtcagcc agtaggcaac tgtagccttt ttgcgggttc cttatgtttg
    21841 aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt ctttctctaa
    21901 atattttaat cagcgagggg atcttcgctg attaaagaaa tagcggtaat gcgttttatt
    21961 cccat
//
LOCUS       DAZRVY010000062        21643 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZRVY010000062 DAZRVY010000000
VERSION     DAZRVY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.062"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHXOX_002426"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHXOX_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR7005870.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHXOX_002427"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHXOX_002427"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR7005871.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHXOX_002428"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHXOX_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR7005872.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHXOX_002429"
     CDS             complement(3717..4511)
                     /locus_tag="ACHXOX_002429"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR7005873.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHXOX_002430"
     CDS             4649..4990
                     /locus_tag="ACHXOX_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR7005874.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHXOX_002431"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHXOX_002431"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR7005875.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHXOX_002432"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHXOX_002432"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR7005876.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHXOX_002433"
     CDS             8906..10198
                     /locus_tag="ACHXOX_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR7005877.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHXOX_002434"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHXOX_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHXOX_002435"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHXOX_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR7005878.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHXOX_002436"
     CDS             complement(10731..11189)
                     /locus_tag="ACHXOX_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR7005879.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHXOX_002437"
     CDS             complement(11186..12103)
                     /locus_tag="ACHXOX_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR7005880.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHXOX_002438"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHXOX_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR7005881.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHXOX_002439"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHXOX_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR7005882.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHXOX_002440"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHXOX_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR7005883.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHXOX_002441"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHXOX_002441"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR7005884.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHXOX_002442"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHXOX_002442"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR7005885.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHXOX_002443"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHXOX_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR7005886.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHXOX_002444"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHXOX_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR7005887.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHXOX_002445"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHXOX_002445"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR7005888.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHXOX_002446"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHXOX_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5013 a         5685 c         5800 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagactaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZRVY010000063        21455 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZRVY010000063 DAZRVY010000000
VERSION     DAZRVY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.063"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHXOX_002447"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHXOX_002447"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR7005889.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHXOX_002448"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHXOX_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR7005890.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="ACHXOX_002449"
     CDS             complement(2378..2992)
                     /locus_tag="ACHXOX_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR7005891.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHXOX_002450"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHXOX_002450"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR7005892.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="ACHXOX_002451"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="ACHXOX_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR7005893.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="ACHXOX_002452"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="ACHXOX_002452"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR7005894.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHXOX_002453"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHXOX_002453"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR7005895.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHXOX_002454"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHXOX_002454"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR7005896.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHXOX_002455"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHXOX_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR7005897.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHXOX_002456"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHXOX_002456"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR7005898.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHXOX_002457"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHXOX_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR7005899.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHXOX_002458"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHXOX_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR7005900.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHXOX_002459"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHXOX_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR7005901.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHXOX_002460"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHXOX_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR7005902.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHXOX_002461"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHXOX_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR7005903.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHXOX_002462"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHXOX_002462"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR7005904.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHXOX_002463"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHXOX_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR7005905.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHXOX_002464"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHXOX_002464"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR7005906.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="ACHXOX_002465"
     CDS             19125..19937
                     /locus_tag="ACHXOX_002465"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR7005907.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21014
                     /gene="tssD"
                     /locus_tag="ACHXOX_002466"
                     /pseudo
     CDS             20331..21014
                     /gene="tssD"
                     /locus_tag="ACHXOX_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            20994..>21455
                     /locus_tag="ACHXOX_002467"
     CDS             20994..>21455
                     /locus_tag="ACHXOX_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7005908.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5265 a         5741 c         5374 g         5075 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcact ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagcggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcagcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcatgt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaagc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAZRVY010000064        21005 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZRVY010000064 DAZRVY010000000
VERSION     DAZRVY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.064"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /locus_tag="ACHXOX_002468"
     CDS             complement(<1..172)
                     /locus_tag="ACHXOX_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR7005909.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="ACHXOX_002469"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="ACHXOX_002469"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR7005910.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="ACHXOX_002470"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="ACHXOX_002470"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR7005911.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="ACHXOX_002471"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="ACHXOX_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR7005912.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHXOX_002472"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="ACHXOX_002472"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR7005913.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHXOX_002473"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="ACHXOX_002473"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR7005914.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="ACHXOX_002474"
     CDS             4290..5153
                     /locus_tag="ACHXOX_002474"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR7005915.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="ACHXOX_002475"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="ACHXOX_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR7005916.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="ACHXOX_002476"
     CDS             6491..7108
                     /locus_tag="ACHXOX_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR7005917.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHXOX_002477"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="ACHXOX_002477"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR7005918.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="ACHXOX_002478"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="ACHXOX_002478"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR7005919.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHXOX_002479"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="ACHXOX_002479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR7005920.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="ACHXOX_002480"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="ACHXOX_002480"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR7005921.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="ACHXOX_002481"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="ACHXOX_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR7005922.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="ACHXOX_002482"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="ACHXOX_002482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR7005923.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHXOX_002483"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="ACHXOX_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR7005924.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="ACHXOX_002484"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="ACHXOX_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR7005925.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="ACHXOX_002485"
     CDS             18074..19783
                     /locus_tag="ACHXOX_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR7005926.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHXOX_002486"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="ACHXOX_002486"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR7005927.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5084 a         5718 c         5386 g         4817 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 acactacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ctgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaac ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg catgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca ccaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAZRVY010000065        20953 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZRVY010000065 DAZRVY010000000
VERSION     DAZRVY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.065"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            138..680
                     /locus_tag="ACHXOX_002487"
     CDS             138..680
                     /locus_tag="ACHXOX_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR7005928.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            780..1982
                     /locus_tag="ACHXOX_002488"
     CDS             780..1982
                     /locus_tag="ACHXOX_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR7005929.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2202..2984
                     /locus_tag="ACHXOX_002489"
     CDS             2202..2984
                     /locus_tag="ACHXOX_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR7005930.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2999..4204
                     /gene="rhmD"
                     /locus_tag="ACHXOX_002490"
     CDS             2999..4204
                     /gene="rhmD"
                     /locus_tag="ACHXOX_002490"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR7005931.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4261..5550
                     /locus_tag="ACHXOX_002491"
     CDS             4261..5550
                     /locus_tag="ACHXOX_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005932.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5568..6371
                     /gene="yfaU"
                     /locus_tag="ACHXOX_002492"
     CDS             5568..6371
                     /gene="yfaU"
                     /locus_tag="ACHXOX_002492"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR7005933.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6412..7314)
                     /locus_tag="ACHXOX_002493"
     CDS             complement(6412..7314)
                     /locus_tag="ACHXOX_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR7005934.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7507..8697)
                     /gene="glpC"
                     /locus_tag="ACHXOX_002494"
     CDS             complement(7507..8697)
                     /gene="glpC"
                     /locus_tag="ACHXOX_002494"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR7005935.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8694..9953)
                     /gene="glpB"
                     /locus_tag="ACHXOX_002495"
     CDS             complement(8694..9953)
                     /gene="glpB"
                     /locus_tag="ACHXOX_002495"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR7005936.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9943..11571)
                     /gene="glpA"
                     /locus_tag="ACHXOX_002496"
     CDS             complement(9943..11571)
                     /gene="glpA"
                     /locus_tag="ACHXOX_002496"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR7005937.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11844..13202
                     /gene="glpT"
                     /locus_tag="ACHXOX_002497"
     CDS             11844..13202
                     /gene="glpT"
                     /locus_tag="ACHXOX_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR7005938.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13207..14283
                     /gene="glpQ"
                     /locus_tag="ACHXOX_002498"
     CDS             13207..14283
                     /gene="glpQ"
                     /locus_tag="ACHXOX_002498"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR7005939.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14325..14567)
                     /locus_tag="ACHXOX_002499"
                     /pseudo
     CDS             complement(14325..14567)
                     /locus_tag="ACHXOX_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14562..14666
                     /locus_tag="ACHXOX_002500"
                     /pseudo
     CDS             14562..14666
                     /locus_tag="ACHXOX_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14746..15395
                     /gene="inaA"
                     /locus_tag="ACHXOX_002501"
                     /pseudo
     CDS             14746..15395
                     /gene="inaA"
                     /locus_tag="ACHXOX_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(15449..15703)
                     /gene="yfaE"
                     /locus_tag="ACHXOX_002502"
     CDS             complement(15449..15703)
                     /gene="yfaE"
                     /locus_tag="ACHXOX_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR7005940.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15703..16833)
                     /gene="nrdB"
                     /locus_tag="ACHXOX_002503"
     CDS             complement(15703..16833)
                     /gene="nrdB"
                     /locus_tag="ACHXOX_002503"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR7005941.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17067..19352)
                     /gene="nrdA"
                     /locus_tag="ACHXOX_002504"
     CDS             complement(17067..19352)
                     /gene="nrdA"
                     /locus_tag="ACHXOX_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR7005942.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19480..19623
                     /gene="ypaB"
                     /locus_tag="ACHXOX_002505"
     CDS             19480..19623
                     /gene="ypaB"
                     /locus_tag="ACHXOX_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR7005943.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            20048..>20953
                     /locus_tag="ACHXOX_002506"
     CDS             20048..>20953
                     /locus_tag="ACHXOX_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR7005944.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT         4924 a         5080 c         5655 g         5294 t
ORIGIN      
        1 ggcgtttaat tatttaagaa gtatttagtt acaataagtg actagatccc ttttttcgct
       61 cattgaatcc tctgtgccag gcagtacaat cccgcagctt tgggaatttt gattaattgc
      121 ttgtgggaaa ggggataatg aaaaaaattg cgctagcagg tctggccgga atgctgttgg
      181 tttctgcatc ggtcaatgca atgagcatca gcggtcaggc gggtaaagaa tacaccaata
      241 ttggtgtcgg ttttgggact gaatcgacgg gcctggcgtt aagcggtaac tggacacata
      301 acgacgacga cggtgacgtc gctggcgtgg ggctggggtt gaatctgcct ctcgggccgt
      361 taatggcgac cgttggcgga aaaggcgttt acaccaaccc gaattacggc gatgaaggtt
      421 atgccgcagc ggtaggaggt ggtttgcagt ggaaaattgg caacagcttc cgtttgtttg
      481 gcgagtatta ctactctccg gattcactct ccagcggtat taaaagttat gaggaagcga
      541 atgctggcgc gcgttacacc attatgcatc cagtcagtat tgaggcgggt tatcgctacc
      601 tgaatctgtc gggtaaagac ggtaaccgcg acaacgccgt ggctgacggc ccgtatgttg
      661 gggttaacgc cagtttctga tcccttccgg cgcggcattc tgtcgcgcca ttcagtttct
      721 tactacgcct tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca
      781 tgttaaaagt ggaaatgtta tccaccgggg atgaagtgtt acacgggcaa atcgttgaca
      841 ctaacgctgc ctggctggcc gattttttct ttcatcaggg gttgccatta tctcgccgca
      901 atacggtggg ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg
      961 ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag tgatgattta agtgcactcg
     1021 ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg
     1081 aacgctattt ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc
     1141 tgcctgccag tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc
     1201 ggcttaatcg ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg
     1261 tcgagcacga aatcctgccg cgcctgcgcg agcgtttttc tttaccacag tcgccggttt
     1321 gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc
     1381 tacaactgcc gccgggtgta acaatgggct atcgctcctc aatgccgatc atcgaactga
     1441 aactcaccgg accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac
     1501 gtgttgccgg acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc
     1561 gcgaattgca aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat
     1621 tggctttgca actttctcgc gcaggtgctc cattgctggc gtgtgaagtg gttccttcac
     1681 aggaagaaac cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg
     1741 ccgggctggc actggctgtt tcgggtttcg agaacgagca tctcaacttt gcgctagcca
     1801 cgccagacgg cactttcgct ctgcgtgtgc gtttcagcac tacacgctac agcctggcta
     1861 tccgtcagga agtgtgcgca atgatggcac tgaatatgct gcgccgttgg ttaaacggcc
     1921 aggatatcgc cagtgagcat ggctggattg aggttgttga gtccatgacc ttatctgtct
     1981 gattccgcca ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaacttgt
     2041 ccaacctgtt gtactcaata agaaaaaatc cgtgcactga ttcacaaatt tgtgcgtaat
     2101 ttctccagtc cgctggacga ggagcgggat ttgatgcgct tcacaatttt actccccctg
     2161 agcaacgaga ctgattccag tatctaagac tggagtccag tatgttggaa tcaagcaaag
     2221 ttcccgccct gacacgggct attgatattc tcaatttgat tgcccgcatt ggcccctgta
     2281 gtgctgcgac gatcattgac acattgggaa tccctaaaag cacggcctat ttgctgctta
     2341 atgaactcag gcgtcagcgt tttcttagcc ttgatcacca ggaaaatttt tgtttgtgga
     2401 ccaggctggt ggagctttct ggtcatgcgt taagcaagat ggatctccgg gaactggctc
     2461 gaccgcgttt gacgcaactg atggatacca ccgggctgtt atgtcacctg gggattattg
     2521 ataacggcag cgcttattac attttaaaag tggagtcatc agccactatc agcgtgcgtt
     2581 cccatgaagg aaaaagcctt tcgctttatc gctccggaat aggtaaatgc ctgcttgcct
     2641 ggcaacctgc agcggtacag caaagtatta tcgaagggct ggtctgggag caggcgacac
     2701 ccacaacgat tactcatcca cagcaacttc acgaagaact ggcgcgtatt cgccgtcagg
     2761 gctggagcta tgacaacggc gaagattacc ccgatgtccg ttgtgtcgct gcgccggtgt
     2821 ttaacgccaa taacgagctt accgctgcga tctcggtggt cggtacccgc ttacaaatca
     2881 atgaagaata ccgtgattat ctggcgggca aggccattgc ctgcgccagg gatatttcac
     2941 gtctgttggg atggaaaagt ccctttgact tacaggcctc ataacaatgg agaacatcat
     3001 gaccctacca aaaatcaaac aggttcgcgc ctggtttact ggcggtgcga cagcagaaaa
     3061 aggtgctggc ggcggtgatt atcacgacca gggggcgaat cactggattg acgatcatat
     3121 tgccaccccg atgagtaaat accgtgatta cgagcagtca cgccagtcat ttggcattaa
     3181 cgttcttggc acgttgattg ttgaagtcga agcagaaaac ggccagaccg gattcgctgt
     3241 ttcgacagcc ggtgaaatgg gctgttttat tgtcgaaaaa catcttaacc gtttcattga
     3301 gggtaaatgt gtcagtgata tcaaactgat ccatgatcaa atgctcaatg ccaccctgta
     3361 ttactccggc tctggtggcc tggtgatgaa cacgatttcc tgtgtcgatc tggctctgtg
     3421 ggatctgttc ggcaaagtgg tcgggcttcc ggtttataaa cttttaggcg gcgcagttcg
     3481 tgatgagatt cagttctacg ccacaggcgc gcgtccggat ctggcaaaag agatgggctt
     3541 tatcggtggc aaaatgccga cgcactgggg gccacatgat ggcgatgcgg ggatccgcaa
     3601 agatgccgtt atggtcgcgg atatgcgtga aaaatgcggt gaggatttct ggttaatgct
     3661 cgactgctgg atgagtcagg acgtgaacta tgccaccaaa ctggcccacg cttgcgcgcc
     3721 ctataacctg aaatggatcg aagagtgcct gccgccacag cagtatgaag gttatcgcga
     3781 actgaaacgc aacgcgccag tcggaatgat ggtcaccagc ggtgagcacc acggcacact
     3841 gcaatctttt cgtacgcttt cagaaaccgg tatcgacatt atgcagccgg atgttggctg
     3901 gtgcggtggt ttaaccacgc tggtggaaat tgccgcaatc gccaaatcca gggggcaact
     3961 ggtggtgccg cacggttcgt ctgtttactc tcaccatgcg gtgatcacct tcaccaatac
     4021 gccattcagc gaattcctga tgaccagccc ggattgttca acgatgcgtc cgcagtttga
     4081 cccgattctg ctcaatgagc cggttccggt gaatggtcgt attcataaat cagtgcttga
     4141 taaacccggt tttggcgtcg aactcaatcg tgactgcaat ttgaaacgcc cctacagcca
     4201 ctaatcactc cgggcgttgc tgtgacaacg gtaacgccca catattctaa ggagcttttt
     4261 atgagcaccg ctttgcttga cgccgtggtg aagaaaaacc gtgtgcgttt aattccgttt
     4321 atgttggcgc tgtatgtgct ggcgtttctc gaccgttcga atatcggttt tgccaaacag
     4381 acctaccaga ttgataccgg gttgagtaat gaagcttatg cgctgggagc aggcattttc
     4441 tttgtggtat atgcgtttct gggtgttccg gcgaatcttt tgatgcgcaa actgggggcc
     4501 agaacctgga ttggtacgac aacactgctg tggggatttc tttcggcagc catggcatgg
     4561 gccgatactg aagcgaaatt tctgatagtt cgcactctgc ttggtgctgc ggaggccgga
     4621 tttttccctg gtatgattta tctcacctcg caatggtttc cgcagcgtaa tcgcgccagc
     4681 attatggggc tgttctatat gggcgcaccg ctggcgttaa cactgggatc accgctttcc
     4741 ggcgcgctgt tggagatgca tggatttatg gggcatcccg gctggttctg gatgtttgtg
     4801 attgaaggat tgttggcagt cggtgctggg gtattcacat tcttttggct tgatgacaca
     4861 ccggagcagg cacgttttct gagtaaacaa gaaaaaacgt tgcttatcaa tcaactggca
     4921 agtgaagaac aacagaaagt gacttctcgg ctgagcgatg cgctgcgtaa tggccgagtc
     4981 tggcaactag cgattatcta cctgaccatt caggtggcgg tttacggatt aattttcttc
     5041 ctgccgaccc aggttgcggc attgctggga acaaaagtgg gctttacagc gtcggtggtc
     5101 accgccattc cgtgggttgc ggccttgttt gggacctggc ttattccgcg ctattccgat
     5161 aaaaccggcg aacggcgtaa tgtcgcagcg ctgacattac tggcggcggg cattggtatt
     5221 ggtctgtccg ggctgctttc tccagtaatg gcgatcgtag cgctgtgtgt tgcagctatc
     5281 gggtttattg ccgtgcagcc ggtgttctgg acgatgccga cacaactgct ttccggtacg
     5341 gcgctggctg cggggattgg ttttgtaaac ctgtttggtg cagtgggcgg gtttattgcc
     5401 ccgatcctgc gcgtgaaagc agaaacgtta tttgccagcg atgcggcagg attactgaca
     5461 ctggcagcgg tggcggtcat cggttcgctg attattttca ctctgcgtgt aaatcgtact
     5521 gttgcgcaga ccgacgtggc acatcattaa ataggttaag gaacacgatg aacgcattat
     5581 taaccaatcc ctttaaagaa cgtttacgca agggcgaagt gcaaattggt ctgtggttaa
     5641 gctcgactac ttcttatatg gcagaaattg ccgctacttc cggttatgac tggttgctga
     5701 ttgacgggga gcacgcgcca aacaccattc aggatcttta tcaccagcta caggcggtag
     5761 cgccctatgc cagccaaccc gtgatccgtc cggtggaagg cagtaaatcg ctgattaaac
     5821 aagttctgga tattggcgcg caaactctat tgatcccgat ggtcgatact gccgatcagg
     5881 ctcgtcaggt ggcgtctgcc acgcgctatc ctccctacgg tgagcgtggt gttggggcca
     5941 gtgtggcacg ggctgcgcgc tggggacgca ttgagaatta catggcgcaa gtcaacgatt
     6001 cgctttgtct gttagtgcag gtggaaagta aaacggcact ggataacctg gacgagatcc
     6061 tcgacgtcga agggattgat ggcgtgttta ttggaccagc ggatctttct gcgtcgttgg
     6121 gctacccgga taacgccggg cacccggaag tgcagcgaat tattgaaacc agcattcggc
     6181 ggatccgtgc tgcgggtaaa gcggctggtt ttctggctgt ggctcctgat atggcgcagc
     6241 aatgcctggc gtggggagcg aactttgtcg ctgttggcgt tgacacgatg ctctacagcg
     6301 atgccctgga tcaacgactg gcgatgttta aatcaggcaa aaatgggcca cgcataaaag
     6361 gtagttattg atatcaaagg cccatgggga tcggctgtgg gcctgtgtta attagtggtt
     6421 attcgctgcc agatcggctt cgcttagctg ggtggccgcg agcacctggt cacgatcaat
     6481 gccctgttcc agcatgcgca aagcaatttt aatggcttgt tcatgcatac cttcctgcca
     6541 gccaatttgg tgtccttcct gccgtagccg ctcagcaata gtcatcaatc tctccttttg
     6601 tagtggtgaa cgttcggaaa tctcctcaat aaaacgggtg aaacgggagg tatcgccgca
     6661 ttgcagcaga taattaaaca gtgtttgtag ctggctgtca ttagtgaagc ctttaaccaa
     6721 aagcgtggtg atcctgtcga ccataccgat taaatcgcgg tcgcgaatat gcttttgaat
     6781 cagttcaagc agagctatac gccgatgttg catgatctca tcgtcaggta cgatggtgat
     6841 atccaccaac ggaaaagctt cggtgtacaa ctgccgggca agttgcggat cgtcaaactc
     6901 atccagccag ttaagtgagt acgggtaggg cgatgtttcg ccatgataaa acagcaatgg
     6961 caccaccagc ggaactctgt catagccttt atccaggtga cgctgcatgg cggcagtggc
     7021 atagcgcatt agccgaaaag ccatattctt ttctgcagag ctttgatgtt caatcacgca
     7081 gtagatatag ccgtcaccgt cgctggtttc cacggaccac aaaacatccg agtagtaagc
     7141 gcgtaaactt ttttcaataa aactagtggg ttccaggcgt aaggtttgca ggttgcaaag
     7201 cttgcgcagt ggttctggca aatgtatttc gagaaaatcc cgtgcggttt cgggtgtgaa
     7261 cataaaggtt ttaaataccg catcatgcgg ggaggaggtt gttgattcgg tcatggttgt
     7321 ccgtcacctg atatttatga tgacgcgaca ttacggttat cccaggcctt tgacatctgc
     7381 cagtttcagc ctttgcgaga cgttttcaga ggaattttca ctgcgttgca acgcatacat
     7441 tgggcacgga atcgtgtacg caaaaatgcc cggtaaccac atgcttaccg ggcatcagaa
     7501 aggagtttaa gccagcgcct gggccagtag cgtaatcgga tgttcgcagc gaagacttgt
     7561 ggacatctca atctgccatt tacaggtttc gcagtcggtg accaccagat ctgcgccgct
     7621 ttcttctatc tggcggaaca gtggtgcgcc gatggcttgt gaggtggggt agttctcttt
     7681 tttgaaaccg taagtacccg caataccgca gcactgggaa tccagcaccg ttaactcaag
     7741 ccccgggatt ttacgcaaca gctccagggt gtagagcgtc cagcccattt tttccatatg
     7801 gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg ttttgccttc
     7861 gtccagcttg cgccacagcc agcgggttgc cagttcgata tgatcgcgca agcctttgtt
     7921 gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt
     7981 ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta cattcgtaat
     8041 tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt agcggtacgc cgcagcattt
     8101 ttctttgctg agcagttgta caccgatacc cattgcgttg agcactttaa ttaaatcttt
     8161 acctaactgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga cctggtcttt
     8221 atattgtgct tgctgagccg ctatgctgcg ataccagcga cggaacgtgc cgaaggagta
     8281 tttcggtagc gtgcgacgat gatcgatttt taacgccgca tcaagcagct gccgcaccgg
     8341 tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc tacccatcag
     8401 gtcggtatga ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat attttgcccg
     8461 cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt cacaacgttt
     8521 gcagttgatg caatatttca gcgcctcgtc atacagtgcg ccatctttca aacgcagacg
     8581 ctcgccatcc ggcccggctt gttttggccc tggataaccg ggattcaccc ggctcaccgg
     8641 gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt cgttcattgt
     8701 tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcactgac ggcacaaaca
     8761 ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc gatggcaaac
     8821 aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg
     8881 aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg
     8941 gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt
     9001 ccgccactaa agaaactgcc gctggcgaga accgcgaaac gtggacgtag cggaatatcg
     9061 gcgtgattgc gggtccagat ttcattcact acgccatttt tacaggtcac ttttttcact
     9121 tcatcgcccg gcatccacac gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc
     9181 agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcagtga acaaggtagt
     9241 ttttcattca accaacgcca cagtttgtca tcggccaggc cgaagcaggc gggcatcagg
     9301 atcatttcgc aggtattggc gacaggaata agcgcatcaa gtaacagcgg ccagttttct
     9361 tcattatcaa ggaaacgggc gatattcacc gcgcgaaatt cggtggcgtt attgcgcagg
     9421 acatccagtt ccggcagctc tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac
     9481 gaagccgccg caagatgcgc ctggaaatcc atcagcccgc ttatccccac tacacatatc
     9541 ttcttcgcgg gtagcggcca gacgggaact tctggcgaac ttaaccaggt tgcacgcaaa
     9601 gtgccgagcg gggtaacgcg ctgatgggcc agttccacgc tgccctgcaa ttgcgcaccg
     9661 ctttcagcga tcaatgcctg cgcctggcaa gcgagatcga gcacgcgttg tggttcgaga
     9721 agactgtaag gatgggctgg tgcctgctga cgcaaagatt ccagtccact gtgaatgtct
     9781 gtcaccggtt gaccatcagg cagatggctc agcaaatcca gcgaaccgga tgagaaatgc
     9841 agcgcgcttt gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg cagttgcagg
     9901 ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg catctttctg
     9961 ctccttctcc agaccacaca gtccctgata aacccagcgg gtaaattcgc tttcgcgcag
    10021 tgcatctccc caggcgatgg gttgcacgcc tttccagcgt tcgttaagga aggtggaaag
    10081 ttgctcgata gattgcgcgg acgtcgtgac gttaaaacgt tgcagcagtc cggcagcgcg
    10141 gcaggcgcag agttcgccct ggcaggtacc catccccaca cgggtacgac ggcgtaaatc
    10201 cagcaggcta ttaacgttta aattttctac cgcgtactgc acttcacccg ctgttaccgc
    10261 ttcgcactca cataccaggc tacggtgcag acgaccttcg ctcagccagg caggcgtgcg
    10321 atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga tgactttacg
    10381 caaggtaact tcagcgggtt cctgtgaacc aggaagtgcc agatcggcgg tcgtacaggg
    10441 gcgcgtgttg cccagtttgc ggcacacggc gtcggtagcc cattcagcca tcagccgata
    10501 ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc gttcagcatg
    10561 gtcgagcagc acgatgccac ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac
    10621 cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag
    10681 tttttcccct tcacgcagca gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat
    10741 ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc gaaatggtat cgccaggcac
    10801 cagaatatcg gcgtcggaag gtttacggca gcggttgatc acatgctggt taatgcggtg
    10861 atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat cggcatattc
    10921 ggcaatgtgt tgcccccaga tcccggcggc attaaccacg acaggcgcat gaagggcctg
    10981 agtttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc cttcacgaat
    11041 cagtcccgtg acttcatgag cggtaaggat aacggcaccg tgttctttgg catccagcat
    11101 gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc ggaactttca ccgcgccaat
    11161 caatgccggg ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc
    11221 gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc tggaaggaga ggtcatcttc
    11281 cggcagggta ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag
    11341 gatctggttt tcactaatgc attcgcgggc cgattccgca tcggttaccg catagcgcgc
    11401 accgctgtgt agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc
    11461 aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc
    11521 gccgccgcca atgataatca cgtcacttga ttgcgagtcg cgagttttca ttgttatccc
    11581 tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt gatttcgcgc
    11641 atattcgctc atgattcgaa agtgaaacgt gatttcatgc gtcattttga acattttgta
    11701 aatcttattt aataatgtgt gcggcaattc acatttaatt tatgaatgtt ttcttaacat
    11761 cgcggcaact caagaaacgg caggttctct cactgaatca ggctgttaat cataaataag
    11821 accacgggcc acggaggcta tcaatgttga gtatttttaa accagcgcca cacaaagcgc
    11881 gcttacctgc cgcggagatc gatccgactt atcgtcgatt gcgctggcaa attttcctgg
    11941 ggatattctt tggctatgcg gcttactatt tggttcgtaa gaactttgcg cttgctatgc
    12001 cttatctggt tgagcaggga ttctcacgcg gtgatttagg ttttgccctt tcggggatct
    12061 cgattgctta tggattttcg aaattcatca tgggttcggt atcggatcgc tcgaatccgc
    12121 gcgttttcct gcccgcaggt ttgattctgg cggcggcagt gatgttgttt atgggctttg
    12181 tgccatgggc gacgtcgagc attgcggtga tgtttgtact gttgttcctc tgcggttggt
    12241 tccaggggat ggggtggccg ccgtgtggtc gtactatggt gcactggtgg tcgcagaaag
    12301 aacgtggcgg cattgtgtca gtgtggaact gtgcgcacaa cgtcggtggt ggtattccgc
    12361 cgctgctgtt cctgctgggg atggcctggt tcaatgactg gcatgcggcg ctctatatgc
    12421 ctgctttctg cgccattctg gtggcattat tcgcctttgc gatgatgcgc gataccccgc
    12481 aatcctgtgg cttgccgccg atcgaagagt acaaaaatga ttatccggac gactataacg
    12541 aaaaagcgga acaggagctg acggcgaagc aaatcttcat gcagtacgta ctgccgaaca
    12601 aactgctgtg gtatatcgcc atcgccaacg tgttcgttta tctgctgcgt tacggcatcc
    12661 tcgactggtc accgacttat ctgaaagagg ttaagcattt cgcgctggat aaatcctcct
    12721 gggcctactt cctttatgaa tatgcaggta ttccgggcac tctgctgtgc ggctggatgt
    12781 cggataaagt cttccgtggc aaccgtgggg caaccggcgt tttctttatg acactggtga
    12841 ccatcgcgac tatcgtttac tggatgaacc cggcaggtaa cccaaccgtc gatatgattt
    12901 gtatgattgt tatcggcttc ctgatctacg gtcctgtgat gctgatcggt ctgcatgcgc
    12961 tggaactggc accgaaaaaa gcggcaggta cggcagcggg ctttaccggg ctgtttggtt
    13021 acctgggcgg ttcggtggcg gcgagcgcga ttgttggcta caccgtggac ttcttcggct
    13081 gggatggcgg ctttatggta atgattggcg gcagcattct ggcggttatc ttgttgattg
    13141 ttgtgatgat tggcgaaaaa cgtcgccatg aacaattact gcaaaaacgc aacggaggct
    13201 aatggcatga aattgaagct gaaaaacctt agcatggcga tcatgatgag cactatagtc
    13261 atgggaagca gtgcaatggc ggcggacagc aacgaaaaaa tagtcatcgc ccatcgcggt
    13321 gccagtggat atttgccgga gcatacgctg ccagcaaaag cgatggcgta tgcgcaggga
    13381 gcggattatc tggaacagga tttggtgatg accaaagacg accatctggt tgttctgcat
    13441 gaccattatc tcgatcgtgt tactgatgtt gccgatcgtt tcccggatcg ggcgcgcaaa
    13501 gacggtcgtt actacgcgat agatttcacg ctggatgaaa ttaagtcgct gaaatttacc
    13561 gaaggtttcg atattgaaaa cggtaaaaaa gtacagactt atccggggcg tttcccaatg
    13621 ggtaagtccg acttccgggg gcacaccttt gaagaagaga ttgaatttgt tcaggggtta
    13681 aatcactcta ccgggaaaaa tatcggtatc tatccagaaa tcaaagcgcc gtggttccat
    13741 catcaggaag ggaaggatat tgcggcaaaa acgctggaag tgctgaagaa atatggttac
    13801 accggtaagg acgataaagt ttatttgcaa tgttttgatg ctgatgagct gaagcgtatt
    13861 aagaatgagc tggaacctaa aatgggcatg gatctcaatc tggtacagct gattgcctat
    13921 accgactgga atgaaacgca gcagaaacag ccggacggaa gctgggttaa ttacagctat
    13981 gactggatgt ttaagccagg tgccatgaag caagtcgcgg aatatgccga cggtatcggg
    14041 ccggattacc atatgttgat tgaggaaaca tcgcagccgg gtaatatcaa actcactggc
    14101 atggtgcaag atgctcagca gaataaactg gtagtgcatc cttataccgt gcggtcggat
    14161 aaactgcctg aatacactac tgatgtgaat cagttatatg atgctctgta taacaaagcg
    14221 ggtgtaaatg ggctgtttac tgatttccct gataaggcag taaaattcct taataaagag
    14281 taatgctact gcaggatggc gttcatgcgc catcctgtct catatcaaat ttttccaatc
    14341 gccactaatt gttccatgca catttgtagt aaaaacagtg gactgccttc taccggcttt
    14401 tctttagcca acaacgaaat ctgtcgatag aaggttggtt cgtgaggaac ggaacacaat
    14461 tcacctgcaa tggttttcag cgttaactct ggtaataagg cgatgcctaa tccctgtcga
    14521 ataaaattca tcgctgtatc cacctgatta aattcacatt ttatctgctg ggcgcactat
    14581 tagtggtcgt ggctggtgga atctatgcgc tgcaaatgaa gggcaaaagc ggtgtcacac
    14641 tagtagcggc aaaagaaatt cattaatacg acacgtttca ttaagatttt cctcaggtaa
    14701 ccacctatag tcattgtcgt taataaaaat gacaggcgat agggtatggc agtttcagca
    14761 aagtacgacg aatttaatca ctggtgggca acagagggcg actgggttga agagcctaac
    14821 tatcgccgta atggtatgag cggggtgcaa tgcgtcgagc gcaacggcaa aaagctgtat
    14881 gtaaagcgca tgacgcatca tctgtttcat tccgtacgtt atccgttcgg ccgaccaacg
    14941 attgtccgtg aggttgcggt aattaaagaa ctggaacggg ccggtgtcat cgtgccgaag
    15001 atcgtttttg gtgaagcggt gaaaattgag ggtgaatggc gcgcgttgct ggtgactgaa
    15061 gatatggcgg ggttcatcag cattgctgac tggtatgccc ggcatgcagt atcgccctat
    15121 tctgacgaag tacggcaagc catgttgaaa gcagtggcgc tggcgtttaa gaaaatgcat
    15181 agcgttaatc gtcagcatgg ctgttgttat gttcgccata tctatgtgaa aacagaaggt
    15241 aaagcagaag ctggttttct tgacctggag aaaagtcgtc gccgcttgcg tcgcaataaa
    15301 gcgattaatc atgacttccg ccagctggaa aatatctgga gccaatcccc aaagcggact
    15361 gggagcaggt gaaagcgtat tactacgcca tgtaatcgtt gtaggccaga taagacgtat
    15421 aagcgttgta tctggcacta caaccaattc acatctcgat ttcaatatcg ccttttgccc
    15481 ggcaacaaca gggcaaaatt tcccccggct gaataaaggc taacggttcg gcaatccagt
    15541 ccacctgacc tgcgaccagt cgggtgcgac aggagccgca gtaaccttcg cggcactggt
    15601 actcaaccgc aacattgtgg gattccagcg ccgccagaag ggaagggtgt tcatcctggc
    15661 acagcagttg tgtgccagtg atgcgcaggg taacgcgggc catcagagct ggaagttact
    15721 caaatcgtcg gtgtccactt ccgcgtcaat ctgcccgacc agataagaac tgacttccac
    15781 ttcctgcgga gcaacctgca cgttatcaga caccagccag gtgttgatcc acgggatcgg
    15841 gttggagcgc gtctggaacg gcagatccaa accgactgcc tgcatgcgga tattggtgat
    15901 gtattcaacg tactggcaga gaatatcttt attcagacca atcatcgaac cgtcacggaa
    15961 cagataatcc gcccagtctt tctcttgctg tgccgcctgg acaaacaggt catagcactc
    16021 ctgcttacat tcttcggcaa tttccgccat ctccggatca tccgcgccgc tgcgcagcag
    16081 attcagcata tgctgagtac cggtcaggtg cagggcttca tcgcgggcaa tcaggcgaat
    16141 aatcttggcg ttaccttcca tcagttcgcg ttctgcaaag gcaaaggaac aagcaaagct
    16201 gacgtagaag cggatcgctt ccagcgcgtt aacgctcatc aggcagagat acagtttttt
    16261 cttcagctcg cgcaggctaa cggttacggt tttaccgtta acggtgtggg tgccttcgcc
    16321 cagaaggtgc cagtagctgg tcatttcgat cagttcatcg taatagctgg agatcccttc
    16381 cgcacgtttc tggatctgct cgttggtgac gatatcgtca aacacaacag acggatcgtt
    16441 aacgatatta cgaatgatat gagtatagga acgggaatga atcgtttctg agaacgccca
    16501 ggtttcgacc caggtttcca gttccggaat agaaataagc ggcaatagcg ccacgttcgg
    16561 gctacgaccc tgaatggaat ccagcagcgt ctgatatttc aggttgctga taaagatgtg
    16621 tttttcgtgc tccggcagcg cctggtaatc tatacggtcg cgggagacgt caacttcttc
    16681 cggacgccag aagaaagaga gctgcttttc gatcagcttt tcgaagatgt catatttttg
    16741 ctgatcgtag cgagccacgt tgaccggctg accaaagaac atcggttctt tgagctgatc
    16801 atttttcgtc tgtgaaaagg tggtatatgc catgagtgtg tcctgttggg agtttaatgc
    16861 cggataaggc attttacgcc gcatcctgtg cctgatgcga cgctggcgcg tcttatcagg
    16921 cctacaaatc cgagccgtag gccggataag gcgttcacgc tgcatccggc acccggagcc
    16981 tgatgcgacg ctggcgcgtc ttatcaggcc tacaaaccga gccgtaggcc ggataaggcg
    17041 tttacgccgc atccggcatc tcaatatcag atcttacatg cgccgctttc gcagccatcg
    17101 tcctggattg acggtaccag atcgtcctgc gcgtcttcag cgccgtcacg ggtgttctga
    17161 taatacagcg ttttgacccc gaatttgtag gcggtgagca ggtctttcag caactgctgc
    17221 atcggcactt ttcctgacgg gaaacgtgac ggatcgtagt tggtgttagc agagatcgac
    17281 tgatcgataa atttctgcat gatacctacc agttgcaggt agccatcgtt acccggcatt
    17341 tcccacagca gctcataggc gtcgtgcagg tgctcgtagt ccggcaccac ctggcgcaaa
    17401 ataccgtctt tcgacgcttt gatgctgacg taaccgcgcg gcggttcaat accgttagtg
    17461 gcgttagaga tctgcgaaga ggtctcggac ggcatcaggg cagaaagcgt agagttacgc
    17521 aggccgtgcg ttttgattga ttcacgcaat gcttcccagt cgtaatgcag cggctcatta
    17581 gcgatggtat ccagatcttt cttataggta tcgatcggca ggatcccttt cgcgtaagtg
    17641 gtttcgttaa accacgggca cgcgccttgc tctttcgcca gctcattaga ggctttcagc
    17701 aggtaatact gaatggcttc gaaggtttta tgcgtcaggt tgttggcgct gccgtcggag
    17761 tagcgtttac cgtgcttcgc caggtagtaa gcgaagttga tcacaccaat acccagcgta
    17821 cgacgaccca tcgctccacg tttggcggcc gggatcgggt aatcctgata atccagcagc
    17881 gcgtcaagtg cacgaaccgc cagaattgcc agctcttcca gttcatccag gttattaatt
    17941 gcgcccaggt tgaaagcaga cagcgtacac agcgcgattt caccgttctc gtcgttgacg
    18001 tcgttcagcg gtttggtcgg cagggctatc tccaggcaca ggttagactg acgcactggc
    18061 gcgatggccg gatcaaacgg gctatgggta ttgcagtggt caacgttctg aatatagata
    18121 cgaccggtag acgcacgttc ctgcatcatc agcgagaaca gctcaacggc tttcacacgc
    18181 tgcttgcgga tgctgtcgtc tttctcatat ttggtataca gacgttcaaa ctcttcctga
    18241 tcggcgaaga acgcgtcgta cagccccggt acgtcggacg ggctgaacag ggtgatatct
    18301 tcacctttca gcagacgggt atacatcagt ttgttgattt gtaccccgta gtccatatga
    18361 cgcacgcggt tgccttccac accacggttg tttttcaaca ccagcaggct ttccacttcc
    18421 agatgccaca tcgggtagaa cagcgttgcc gcaccgccgc gcacaccgcc ctgagagcag
    18481 gatttcaccg ctgtctggaa atgtttgtag aacggaatgc agccggtatg gaacgcttca
    18541 ccaccgcgaa tcgggctacc cagcgcacga atacgcccgg cgttgatgcc gatcccggca
    18601 cgctgggaaa cgtatttaac aatcgcgctg gaggtggcgt tgatggaatc caggctgtca
    18661 ccgcactcga tcagtacgca ggagctgaac tgacgagtcg gggtacgcac gccggacatg
    18721 attggcgtcg gcagcgaaat tttaaatgtg gaaaccgcgt cgtaaaaacg cttcacatat
    18781 tgcaggcgcg tttcacgcgg gtagttcgag aacaggcacg cggcaaccag aatataaagg
    18841 aactgggcgc tctcatagat ttcgccggtc acgcggttct gtaccagata tttgccttcc
    18901 agctgcttaa cggcagcata agagaaggtc atatcacggt cgtgatcgat aaacgtgtcc
    18961 atctgcttga actcttcttc cgtgtagtct tccagcagat gattatcgta tttgcccatc
    19021 tcgaccattt tcaccacgtg gtcgtacagc gcaggcggct caaactggcc gtaggctttt
    19081 ttacgcaggt ggaagatcgc caggcgcgcg gcgagatact gataatccgg cgcatcacgg
    19141 gagatcaggt ctgcggcagc cttgataatg gtttcgtgga tgtcagaggt cttgataccg
    19201 tcataaaact gaatgtggga gcgcagctcg acctgggaaa tcgaaacgtt atgcagtcct
    19261 tctgccgccc aatccagaac gcgatggatt ttgtcgagat tgatgcgctc tgtgctaccg
    19321 tcgcgctttg tcaccagcag attctgattc atgtatgtcg tacctgtttt tggaattctt
    19381 tccgcaatag tttcatgcag actacctgta gtaagggaag ctgcttagaa cactatatat
    19441 agggggtatg tttgggattc actgcaagat agtgtgaaaa tgaccctctt gcaagtgcat
    19501 aactttgtgg ataactcagg aaggaaaaag tggctttcgc gcaccttagg tcagacaagg
    19561 tgtccgggaa agtcaatggg aagagaaaaa tttgttaaaa ataactgttc ggcgatttct
    19621 tgaacggcag aataggttgg ataaattgat atagatggtt tattaatctt tttgttaatg
    19681 attgcaaaaa ttgatctaag cacgagtcat cacataatct gatgtatcag ttgtggtcaa
    19741 tatataagcg aatgtaagcg attgttgatg tcgaaatggt tgttttctat tcggttattg
    19801 ttaaatgcga agccattttg ttaatttacc ttatattata agaaaattat tcattactat
    19861 aatttagttt aaatataact ggtatccctt aaggttatta acttgaatta tgacgcactg
    19921 atattattca tcaaataata acaaaatagc cattgcatcg ggttggacaa tttacaaaag
    19981 aaagataacc catattgtat aatgcgttta aatatgccgt cttatataaa atgatggtca
    20041 gattaatatg cggattatct ttctacgcaa ggagtattta tctctactcc cgtcaatgat
    20101 tgcatctctt ttctctgcta acggtgtcgc ggcggccatt gatttatgcc agggatatga
    20161 tatcaaagcg agttgtcacg ccagcaggca aagcctttca ggcattacgc aggactggag
    20221 tatcgccgat gggcaatggc tggttttttc ggatatgacc aataatgcca gcggtggggc
    20281 cgtatttttg caacaaggag cggaatttac attatcacca gaaaacgaaa ctggaatgac
    20341 tctgtttgcc aataacaccg ttacaggaga atattataac ggcggggcca tatttgctaa
    20401 agaaaactca acgctgaatc ttactgatgt cattttttcc ggtaacgtcg caggcggcta
    20461 tggtggcgca atctattctt ctggtactaa cgatactggt gccgtcgatt taagtgtcac
    20521 taacgccatg tttcgcaata atatcgctaa tgatggcaaa ggtggcgcaa tttataccat
    20581 taataatgac gtctatttaa gtgatgttat ttttgataac aaccaggcat atacatcaac
    20641 aagttacagt gatggcgatg gcggggcaat cgatgttacc gataataata gcgacagcaa
    20701 gcatcctcca ggttatacga taataaataa cactgccttt acaaataaca ctgccgaagg
    20761 ttatggcggg gcgatatata ccaatagcgc gacggctccc tatcttattg atatttctgt
    20821 tgatgacagc tacagccaga acggcggcgt gttagtggat gagaacaaca gcgcagcagg
    20881 ctatggagat ggtccttcca ctgcggcggg tggctttatg tatctcggct taagtgaagt
    20941 tacctttgat att
//
LOCUS       DAZRVY010000066        20427 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZRVY010000066 DAZRVY010000000
VERSION     DAZRVY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.066"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHXOX_002507"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHXOX_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR7005945.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHXOX_002508"
     CDS             433..621
                     /locus_tag="ACHXOX_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR7005946.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHXOX_002509"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHXOX_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR7005947.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHXOX_002510"
     CDS             816..989
                     /locus_tag="ACHXOX_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR7005948.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHXOX_002511"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHXOX_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHXOX_002512"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHXOX_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR7005949.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHXOX_002513"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHXOX_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR7005950.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHXOX_002514"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHXOX_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR7005951.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHXOX_002515"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHXOX_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR7005952.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHXOX_002516"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHXOX_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHXOX_002517"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHXOX_002517"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR7005953.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHXOX_002518"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHXOX_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR7005954.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHXOX_002519"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHXOX_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR7005955.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="ACHXOX_002520"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="ACHXOX_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="ACHXOX_002521"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="ACHXOX_002521"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR7005956.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="ACHXOX_002522"
     CDS             complement(15250..15477)
                     /locus_tag="ACHXOX_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR7005957.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHXOX_002523"
     CDS             complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHXOX_002523"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR7005958.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16467..16640
                     /locus_tag="ACHXOX_002524"
     CDS             16467..16640
                     /locus_tag="ACHXOX_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR7005959.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHXOX_002525"
     CDS             complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHXOX_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR7005960.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHXOX_002526"
     CDS             complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHXOX_002526"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR7005961.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18752..19342)
                     /locus_tag="ACHXOX_002527"
     CDS             complement(18752..19342)
                     /locus_tag="ACHXOX_002527"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR7005962.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHXOX_002528"
     CDS             complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHXOX_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR7005963.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHXOX_002529"
     CDS             complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHXOX_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR7005964.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         5106 a         5200 c         5243 g         4878 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tatatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc
    16081 agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac
    16141 cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat
    16201 ttataacaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg
    16261 cgttttttta atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc
    16321 taagaagctg ttgcaggata ttaccaaacg cgggtctacc cgcgtcagtt cactaagctt
    16381 agtcccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga
    16441 tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg
    16501 cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta
    16561 atttcaagaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc
    16621 acggcaaaag cgacaactag ccgggctaat caatgacgaa tgcatttttg tctgtagctc
    16681 gtcaaaaagc catcaccgcc ggttacccgg tggttgatac tgatgacaaa tgtaagcttg
    16741 cctgatgcgc gatgcttatc aggcctacca gaagattgca atatattgaa tttgcactgt
    16801 tttgtaggcc ggataaggcg tttacgccgc atccggcatg aacaatgcgt acgttgtcaa
    16861 caatctgcac cgccgggtaa ccccggcggt tttctgttta tggctcctga tgaacaactt
    16921 ctggcggtgg aacgtcaacc aaccttctac ttaacaacgc attgagtaaa atcgcgccaa
    16981 aggtggctgt accgatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    17041 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    17101 ggttttgtac ccagatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    17161 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
    17221 ccaacagcat ggcgatgacg gcagcagcaa caaacaccag cgtcgagtag acttttgtca
    17281 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
    17341 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
    17401 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    17461 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    17521 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    17581 cgttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    17641 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    17701 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    17761 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    17821 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    17881 tcccgcccag tgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    17941 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
    18001 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    18061 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    18121 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
    18181 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
    18241 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    18301 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    18361 gacgctcacc acctggctga tgcggcagtc aaacgaaacc agagcctctt ccagttgcgg
    18421 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    18481 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    18541 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    18601 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggatgg
    18661 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
    18721 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
    18781 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    18841 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    18901 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct
    18961 gcatggagct gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    19021 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
    19081 caacgatagc ggtgacgggc gcggtcaggg ttttttgtag attgccgctg gaaagcgccg
    19141 ggtgcagacg ttcttttcct tctacagtgc ggataaacac gatccgtgcc ggagaacagt
    19201 tagctgatgt cggcccccat tttatcgggg cataaagctc ccgtaacgtc tcatcgctga
    19261 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
    19321 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    19381 ggctggcaag cccgttgagt aacagagcat taaacgtttc ggggtcggtc acgttgcagg
    19441 cgtgtccgcc atagcgcatc accattttct ggctatcggg cagggcggca tgaagctcac
    19501 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    19561 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    19621 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctca aggcaaggtg
    19681 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    19741 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    19801 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    19861 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    19921 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    19981 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    20041 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    20101 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    20161 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    20221 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    20281 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    20341 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
    20401 tttgggtcat cggcaaacag cacgtta
//
LOCUS       DAZRVY010000067        20041 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZRVY010000067 DAZRVY010000000
VERSION     DAZRVY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.067"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHXOX_002530"
     CDS             79..213
                     /locus_tag="ACHXOX_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR7005965.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHXOX_002531"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHXOX_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR7005966.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHXOX_002532"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHXOX_002532"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR7005967.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHXOX_002533"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHXOX_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR7005968.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHXOX_002534"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHXOX_002534"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR7005969.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHXOX_002535"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHXOX_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR7005970.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHXOX_002536"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHXOX_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7005971.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHXOX_002537"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHXOX_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR7005972.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHXOX_002538"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHXOX_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR7005973.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHXOX_002539"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHXOX_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR7005974.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHXOX_002540"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHXOX_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR7005975.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHXOX_002541"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHXOX_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR7005976.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHXOX_002542"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHXOX_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="ACHXOX_002543"
     CDS             complement(11620..13284)
                     /gene="glnS"
                     /locus_tag="ACHXOX_002543"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR7005977.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="ACHXOX_002544"
     CDS             complement(13543..15489)
                     /gene="nagE"
                     /locus_tag="ACHXOX_002544"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR7005978.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            15822..16622
                     /gene="nagB"
                     /locus_tag="ACHXOX_002545"
     CDS             15822..16622
                     /gene="nagB"
                     /locus_tag="ACHXOX_002545"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR7005979.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16682..17830
                     /gene="nagA"
                     /locus_tag="ACHXOX_002546"
     CDS             16682..17830
                     /gene="nagA"
                     /locus_tag="ACHXOX_002546"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR7005980.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            17839..19059
                     /gene="nagC"
                     /locus_tag="ACHXOX_002547"
                     /gene_synonym="nagR"
     CDS             17839..19059
                     /gene="nagC"
                     /locus_tag="ACHXOX_002547"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR7005981.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            19107..19859
                     /gene="nagD"
                     /locus_tag="ACHXOX_002548"
     CDS             19107..19859
                     /gene="nagD"
                     /locus_tag="ACHXOX_002548"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR7005982.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(19910..20041)
                     /locus_tag="ACHXOX_002549"
                     /pseudo
     CDS             complement(19910..20041)
                     /locus_tag="ACHXOX_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4910 a         5065 c         5034 g         5032 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt tttgttattt
    11341 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    11401 acgccgctga acgcggcgtt ttggattgtt tgcatatttc ttgccggatg cggcgtgaac
    11461 gccttaaccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca tgtaggcctg
    11521 ataagcgtag tgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc cttgccggat
    11581 gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta ctttcgccca
    11641 ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag aatggcggct
    11701 atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa ccgcatcttt
    11761 cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa tcaccgacag
    11821 gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcgtaca gacggatttc
    11881 aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt tacgaccatc
    11941 tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg tgatattgcc
    12001 ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca gacgcacttc
    12061 tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat cggcgcgatc
    12121 gatccaaatc tcaccgctaa acggcacctg gcggctaccc atttccggtt tgttcggatg
    12181 gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca gtttcaccgg
    12241 atcgataacc gccattgcgc gcggggcgtt ttcgttgaga tcttcacgga tgcaggattc
    12301 cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt tgcagaactc
    12361 acgaatagaa gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg gcatacgcgg
    12421 gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac gcttggacat
    12481 cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa caggaatggt
    12541 gatgttgtcc agcacccagt cgtacaggcg acggttgtcc tggaactcaa gcgtacacag
    12601 agagtgcgta ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt acatcgggta
    12661 gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt acagcaccgg
    12721 atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc aggctttacc
    12781 ttcttcaaaa ccaccggcac gcattttttc gaacagcgcc aggttctctt caacgctgcg
    12841 gtcgcggtac gggctgtttt tacccggttg cgtcagggtg ccgcggtatt cgcggatctg
    12901 ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga tcgcataggc
    12961 gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt gaaaacctaa
    13021 ccactctacg tcgtttttga tcgactcaac atactcgata tcttctttta ccgggttagt
    13081 gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga agttcaggca
    13141 gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga aacgggtgtg
    13201 tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga taaagttagt
    13261 cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac gtataacggc
    13321 gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaatc gaaaatctca
    13381 ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc caccgctttt
    13441 gccagtcccg cgtcattgtt ggatgcgcgt cttatccaac caggactgag cttttgtagg
    13501 cctgataaga cgcgtcaagc gtcgcatcag gcataaagca gattactttt tgatttcata
    13561 cagcggtgtt tgacccgcca caacattgcc ctgagcttta atgatcaagc cactgaaatc
    13621 gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt tcaggtaatc
    13681 cagatccatt tccagaatcg gttgccctgc gctcacctgc gcgccctctt ccaccagacg
    13741 tttaaagcct ttaccttcca gcgctacggt gtcgataccc atgtggacga caatctccgc
    13801 gcctttttcg gtttctaggc agaacgcgtg gttggtgttg aagattttca cgattgtccc
    13861 tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt cacccaccgc
    13921 tttgctggcg aatgcttcgt cagaaacctg atccagtgcc acgacatcac cggtaatcgg
    13981 cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg ctacaggagc
    14041 ggcagttgcc ggagttgctt cagctgacgc agctgctacc gggccacggg caacgacttt
    14101 cttcatcgca tcgccaatgg attctgcttt cgcgccaaca atcacctgaa tggtctgttt
    14161 gttcagtttt actacaccag aagcacccag acgtttacac atcgcatcgt tgacgcgagc
    14221 tgagtcagcc acggtaaggc gcagacgggt aatacaggcg tcaatcgctt tcaggttgtc
    14281 agtaccgcca accgcagcaa tatagttggt tgccagttga gtcagacctt cttcagtatt
    14341 gctgttagcc tcttcagtta cgatctcgtc ttctttatct tcacgacccg gcgttttcag
    14401 gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga agacaacacc
    14461 catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca tcaacgcgta
    14521 gtcgatagcc cccgcagaga aagagaagcc agcatggata cccagcagcg ttgccacaaa
    14581 caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa ggaacatgaa
    14641 caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa cagaaagcag
    14701 catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg ccagcgccgc
    14761 acccggcaga ccgaacatca tgatcgggaa gaagccggac atgaacatcc ccgcggtgcc
    14821 gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg cgttggtgaa
    14881 ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg ttgggatcag
    14941 cagacggttg atgaaaccaa agataccgga acccagcgcg cctgcagaaa cgatccactc
    15001 gccgcctgca tggatagcgt gctgtactgg cggccagacg taaccaaaaa tggccgccag
    15061 caccaggcag aagaagccgg tggcaatcgg cacaaagcgt ttgccgccga agaagctcag
    15121 gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa ccagaccggt
    15181 aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca tcgctttggt
    15241 taacacaaag taacctaccg cacccgccag cgccgccgca cctgcgctgt ctttcgacca
    15301 gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg caccgcccgc
    15361 ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca acagtgccgc
    15421 caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga aaaaacctaa
    15481 aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc cgtattttta
    15541 ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca tctgctacag
    15601 agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gccttttgtg agttttgtca
    15661 ccaaatatcg ttatcatcac tcccttttac tggctaaagc agaaaactta ttttatcatt
    15721 caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct taaagatgcc
    15781 taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg atccccctaa
    15841 ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt atcaatgcgt
    15901 tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc gactggcggc acgccgatga
    15961 ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt aagcacgttg
    16021 tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa agctactaca
    16081 gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac atcaaccttc
    16141 tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa aaaatccgtt
    16201 cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat attgcattta
    16261 acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact catgacactc
    16321 gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa tatgccctga
    16381 ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg ctgggtagcc
    16441 agaaagcact ggcactgcag gctgcagttg aaggttgcgt gaaccacatg tggaccatca
    16501 gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc accatggagc
    16561 tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc aaaggtctgt
    16621 aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat cggggggcag
    16681 aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg atgaccacgc
    16741 ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac tgccgccaga
    16801 gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg atgtgcagtt
    16861 aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg tggaaacgct
    16921 ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc cgacgcttat
    16981 caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt acctggcaag
    17041 acatccgaat caggcgttag gtctgcatct ggaaggtccg tggctgaatc tggtaaaaaa
    17101 aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg atttcctgtg
    17161 tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc ctgcggaagt
    17221 catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca acgcgacgtt
    17281 gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc tgtacaacgc
    17341 gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg acgaagctga
    17401 catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca ttcgtaacgc
    17461 taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc cagcaggtgc
    17521 caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg gactttgtgt
    17581 ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg tgcgtaatct
    17641 ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc tctatccggc
    17701 gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag tagccaacct
    17761 gacctcattc acacctgatt ttaaaatcac caagaccatc gttaacggta acgaggtcgt
    17821 aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa tgttgatctc
    17881 gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg gccaatctcg
    17941 cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa aattacgcgt
    18001 cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac cgggggccgc
    18061 cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt acggcttggt
    18121 cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc agaagaacat
    18181 tacccgctgc cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa tgccattgct
    18241 cagtttattg atagctacca gcgcaaactg cgcgagctga tcgcgatttc ggtgatcctg
    18301 ccagggcttg ttgacccgga cagcggcaaa attcattaca tgccgcatat tcaggtagaa
    18361 aactgggggc tggtagaagc tctggaagaa cgttttaaag tgacctgttt cgttggtcac
    18421 gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga ttgcgaagac
    18481 tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa cgggcgcatt
    18541 tttattggcc gcaacggtaa cgtcggtgaa attggccata ttcaggtcga accgctgggt
    18601 gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa cgctgccatt
    18661 gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc gctggacgac
    18721 tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagtctggc gtcggaagta
    18781 attgagtatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa cttatttaat
    18841 ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct gctccctgct
    18901 attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct gccggtggta
    18961 cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa acgcgccatg
    19021 ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat agtggcgctt
    19081 attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc gatatcgacg
    19141 gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg cacgggatta
    19201 tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc gggcaagatc
    19261 tggcgaaccg ctttgccact gccggtgtcg atgtgccgga tagcgtgttt tatacctctg
    19321 cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcatat gtggtgggcg
    19381 aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat gtgaaccctg
    19441 atttcgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat aaagcggcct
    19501 atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac gggcgcggtt
    19561 tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa aatctccggg cgcaaaccgt
    19621 tctatgttgg taagcccagc ccgtggatca tccgcgcagc attaaacaaa atgcaggcgc
    19681 attcggaaga aacggtgatt gtcggcgata acttgcgcac cgacattctg gcgggcttcc
    19741 aggcaggtct ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac gatatcgaca
    19801 gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc tgaaatcgac gttatctgaa
    19861 taccacccac gtctgcggac gtgggtggct ctgatgacaa acgccaaact gcctgatgcg
    19921 ctacgcttat caggcctacg cagctcctgc aatatattga attttcatgc ttttgtaggc
    19981 cggataaggc gttcacgccg catccggcat gaacaaatcg cactttatca gcaatttggc
    20041 c
//
LOCUS       DAZRVY010000068        19857 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZRVY010000068 DAZRVY010000000
VERSION     DAZRVY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.068"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            306..1076
                     /locus_tag="ACHXOX_002550"
     CDS             306..1076
                     /locus_tag="ACHXOX_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR7005983.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1067..1540
                     /locus_tag="ACHXOX_002551"
     CDS             1067..1540
                     /locus_tag="ACHXOX_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR7005984.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1648..2187
                     /gene="dnaT"
                     /locus_tag="ACHXOX_002552"
     CDS             1648..2187
                     /gene="dnaT"
                     /locus_tag="ACHXOX_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR7005985.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2190..2927
                     /gene="dnaC"
                     /locus_tag="ACHXOX_002553"
     CDS             2190..2927
                     /gene="dnaC"
                     /locus_tag="ACHXOX_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR7005986.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2976..3470
                     /gene="yjjA"
                     /locus_tag="ACHXOX_002554"
     CDS             2976..3470
                     /gene="yjjA"
                     /locus_tag="ACHXOX_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR7005987.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3724..6015
                     /gene="opgB"
                     /locus_tag="ACHXOX_002555"
     CDS             3724..6015
                     /gene="opgB"
                     /locus_tag="ACHXOX_002555"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR7005988.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHXOX_002556"
     CDS             complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHXOX_002556"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR7005989.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7315..8229
                     /locus_tag="ACHXOX_002557"
     CDS             7315..8229
                     /locus_tag="ACHXOX_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR7005990.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8444..9805
                     /locus_tag="ACHXOX_002558"
     CDS             8444..9805
                     /locus_tag="ACHXOX_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7005991.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9854..11509)
                     /gene="tsr"
                     /locus_tag="ACHXOX_002559"
                     /pseudo
     CDS             complement(9854..11509)
                     /gene="tsr"
                     /locus_tag="ACHXOX_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11628..12074)
                     /gene="hpaR"
                     /locus_tag="ACHXOX_002560"
     CDS             complement(11628..12074)
                     /gene="hpaR"
                     /locus_tag="ACHXOX_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR7005992.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12346..13635
                     /gene="hpaG"
                     /locus_tag="ACHXOX_002561"
     CDS             12346..13635
                     /gene="hpaG"
                     /locus_tag="ACHXOX_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR7005993.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            13632..15098
                     /gene="hpaE"
                     /locus_tag="ACHXOX_002562"
     CDS             13632..15098
                     /gene="hpaE"
                     /locus_tag="ACHXOX_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7005994.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15100..15951
                     /gene="hpaD"
                     /locus_tag="ACHXOX_002563"
     CDS             15100..15951
                     /gene="hpaD"
                     /locus_tag="ACHXOX_002563"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR7005995.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15961..16341
                     /locus_tag="ACHXOX_002564"
     CDS             15961..16341
                     /locus_tag="ACHXOX_002564"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR7005996.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16452..17255
                     /gene="hpaH"
                     /locus_tag="ACHXOX_002565"
     CDS             16452..17255
                     /gene="hpaH"
                     /locus_tag="ACHXOX_002565"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR7005997.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17266..18054
                     /gene="hpaI"
                     /locus_tag="ACHXOX_002566"
     CDS             17266..18054
                     /gene="hpaI"
                     /locus_tag="ACHXOX_002566"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR7005998.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18229..19605
                     /gene="hpaX"
                     /locus_tag="ACHXOX_002567"
     CDS             18229..19605
                     /gene="hpaX"
                     /locus_tag="ACHXOX_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR7005999.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19615..>19857
                     /locus_tag="ACHXOX_002568"
     CDS             19615..>19857
                     /locus_tag="ACHXOX_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR7006000.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4738 a         5354 c         5122 g         4643 t
ORIGIN      
        1 cacaaccaat atttattcaa tgaaatctga tggttttaac ttgttaacat taaaaaacgt
       61 gcccggttat ctattatcct gattcgtaaa ggccaaacaa taaattcatt atagtgataa
      121 tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac cgagcttgtc
      181 ccgataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa caaccgtgct
      241 tcatgataca aatggattct cattatctga acaatacgca gcacgtctat gacaaagggc
      301 gagttatgca aactgagcaa cagcgagccg taacacggct ttgtatccag tgtggattat
      361 ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca cgactgggtc
      421 gggcgttggg gatggacagc gtcgaaagtt ctatctcttc gaacgccata gtgctgacaa
      481 ccattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc ggcattaata
      541 tgcatgtggt gactgaagtc cagcacattg tgattctggc ggagcaccat ctgctggatt
      601 acaaaggcgt agagaaacga tttagccaaa ttcagccatt acgttaccca agatggctgg
      661 tagccttaat ggttggcctt tcttgcgcct gtttctgcaa actcaataaa ggtggctggg
      721 atggtgccgt catcaccttc tttgccagta cggccgcgat gtatatccgc cagctgctgg
      781 cacaacgtca tcttcatccg cagatcaact tttgcctcac cgctttcgcc gccaccacca
      841 tttccggatt gcttttgcaa cttcccactt tcagcaatac acccactatc gcgatggccg
      901 ccagcgttct gctgctggtg ccgggatttc cgttgattaa tgccgtcgcc gatatgttta
      961 aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg acactggcta
     1021 cctgcgtcgg cgtggtgatg gcactgacga tttgggggct acgcggatgg gtgtgatcga
     1081 gtttctgttt gcattggcgc aggatatgat cctcgccgcc attccagcgg tcggctttgc
     1141 gatggtgttc aacgttcccg ttcgggcatt acgctggtgt gcgctgcttg gcgcgattgg
     1201 tcatggttca cgaatgatct taatgaccag cgggctgaat attgagtggt caacctttat
     1261 ggcctccatg ctggtcggca ccattggtat tcaatggtcg cgctggtatc tggcgcatcc
     1321 gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg cttataccgc
     1381 aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga tgattaccct
     1441 gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg gtctttccat
     1501 tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg cttaaagcac
     1561 acggatgaga gacagcctcc tctcctccgt gtgttactat aaaagttatc tcccttctcg
     1621 ttcatcgttc catatttgag aaacagtatg tcttccagag ttttgacccc ggacgtcgtt
     1681 ggtattgacg ccctggtaca cgatcaccaa accgttctgg caaaagctga aggcggtgtg
     1741 gttgccgtat ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc acgcctggct
     1801 gaactgctgg cgctggaaga aaagctggcg cgtccgggaa gcgatgtcgc tctggacgat
     1861 caactctatc aggaaccgca agccgctccc gttgctgtac ccatggggaa attcgccatg
     1921 tatccggact ggcaacccga tgccgatttt atccgcctgg cggcgctatg gggtgtggca
     1981 ctaagagagc cggtgaccac cgaagaactg gcctcattca ttgcctactg gcaggcggaa
     2041 ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt
     2101 cgtgccagca acggcggact gccgaaacga gatgtgaata cggtcagcga acctgacagt
     2161 caaattccac caggattcag agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc
     2221 tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa aacgggtgaa gaactactgg
     2281 cctggcagaa agaacaagga gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga
     2341 tgaaaatgca gcgcaccttt aaccgctccg gtattcggcc actgcatcag aactgctcct
     2401 ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt
     2461 acgtcgaaga gtttgacggc aacatcgcca gcttcatctt ttctggtaag ccaggaaccg
     2521 gcaaaaacca tctggcggcg gcaatctgca acgagctgct gctacgcggt aaatccgtat
     2581 tgatcatcac cgtggccgat attatgtcgg cgatgaaaga taccttcagg aatagcggta
     2641 ccagtgaaga acaactgctt aacgatctga gcaacgttga tctgctggtg atcgatgaga
     2701 tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc
     2761 gctcttcttc caaacgccca accgggatgc tgaccaacag taatatggaa gagatgacca
     2821 agttgctggg cgaacgcgtg atggaccgta tgcgcctggg taacagtttg tgggtgatct
     2881 tcaactggga tagctaccgt agccgggtaa caggtaaaga gtattaatcc ccgtttctgg
     2941 cggtatactg cgacacaatt ccaccttgag taatgatgat gaaaactgtt aaacatcttc
     3001 tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga
     3061 aagatgcgct ctccagcgcc gccagcgaac ttggcaacca aaacagcacg acacaggaag
     3121 gcggttggtc gctcgcgtca ttaactaact tgcttagcag cgggaaccag gccttaagcg
     3181 cagataacat gaacaacgcc gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt
     3241 cggtaaccga tgccgaaaac atcaagaacc aggtgctgga aaagctgggc ctgaacagtg
     3301 aagagcaaaa agaagacacc aactatctgg atggcattca gggtttgctg aaaaccaaag
     3361 atggtcagca actcaatctg gataacatcg gaacgactcc gctggcagaa aaggtgaaaa
     3421 ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt catttcctga accgcgaaac
     3481 gccgcgtttt agctgttacc cggctgacgc ggctctctcc tgcccttttc cctgctcctg
     3541 gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtctg tttataaact
     3601 aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg cgacgccatt
     3661 acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc
     3721 ccgttgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg
     3781 aaagcgggac gtaacacctg gtggtttgca gccacattaa cggtgctggg gctatttgtc
     3841 gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat taacgacgcg
     3901 gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata catcctgccg
     3961 ggtatcggca ttgtgctggg gctgacagcg gtgttcggtg cgctgggctg gatcctgcgc
     4021 cgtcgtcgcc atcatccgca ccattttggt tacagcctgc tggcgctctt actggcgctg
     4081 ggttcggtgg acgccagccc ggcatttcgt cagataacgg aactggtgaa atcccagtca
     4141 cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac tatccctgac
     4201 ccgaaactca acctggttta tatctacggc gaaagtctcg agcggaccta ttttgataac
     4261 gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg cctggatttc
     4321 agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt ggcttctcag
     4381 tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc
     4441 ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca gaactatttc
     4501 gtgcagggcg cgaatctgcg ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc
     4561 gaccacttat acggctcaga agagctgaaa agcgtggtgg ccgacccgca ctatcgcaac
     4621 gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt
     4681 tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca tcacccggat
     4741 ggttttatct cgcgtacctg taaccgcaaa aaatatgatt ttgacggtaa gccgaaccag
     4801 tcattcagcg cggtaagttg cagccaggag aatatcgcgg cgtttatcaa caaaatcaaa
     4861 gcgtcaccgt ggtttaaaga taccgtcatc gtcgtctctt ctgaccattt agcgatgaac
     4921 aacacggcgt ggaaatacct caataagcag gatcgcaata acctgttttt tgtcattcgt
     4981 ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta acacgatgga taacggcgcg
     5041 acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag cagtttatcc
     5101 gggcagtcga tgtcggaaat cttcctcaat atcaaagaga aaacattggc gtggaagccg
     5161 gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat cgaccagcag
     5221 aaaaacatga ttgccttctc gggtagccat ttccgtttgc cgctgctgtt gcgggtttca
     5281 gacaaacgcg tggaaccgct gccggaaagc gaatactcag caccgctgcg tttccagctg
     5341 gccgatttcg ctccacgcga caatttcgtc tgggttgacc gttgctataa gatggcacaa
     5401 ctctgggctc cggaactggc actctccacc gactggtgtg tctcgcaagg gcagcttggc
     5461 ggtcagcaaa ttgttcagca tgttgacaaa acaacatggc agggcaaaac ggcatttaaa
     5521 gatacggtca tcgacatggc gcgttataaa ggcaatgtcg atacgctgaa aattgttgat
     5581 aacgatattc gctacaaagc cgacagtttc atcttcaacg tcgccggtgc gccagaagag
     5641 gtgaaacagt ttagtgggat ttcccgtcca gagtcgtggg gccgctggtc caacgcgcag
     5701 ctgggcgatg aagtaaaaat cgagtacaag catccgctgc caaagaaatt tgacctggtg
     5761 attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg cgtaggcaat
     5821 gaagaacaaa cccttgtgct gggcaatgaa gtgaccacca ccacactgca tttcgataac
     5881 ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg
     5941 aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat taaagtggtg
     6001 gaacgtgaag gataattcct gactgatgtg taggtcgcat aaggtgcgac ctacacacct
     6061 tctgcggcct acaaaacatc attgagaaac agaaaaaaac agcgcccaac acttccccgg
     6121 acgctgcatg gcaactttaa taatctcgcc ttatcagaaa gtaattacac ccttaatcaa
     6181 ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga acggatagcg
     6241 atgggttaac atcatgtcag cagtgatttt tccttccgcc atcagtcgac cgactttagc
     6301 aaaatcttcc ggcgtggcgt tgcggctacc catcatcgtc gtttcttttt tatggaattc
     6361 cggatcggag aactgcaact cacctttaaa caggccgaca aataccaccg tgccgccgtg
     6421 acgaatcaaa ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt cgatcacttt
     6481 ctgcgccagc gaaccaccaa actgcgcccg tagctgcgcg tcaaaatctt cggctgacgg
     6541 gtccagtaaa ggtaattcca gacgcgttgc cacatgttca cggcgcgccg gactggtatc
     6601 cgccaccacc acctgtgcgc catcggcttt agcgattgcc gccgcgccca gaccgattgg
     6661 ccccgccccg accaccagca cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg
     6721 agcgctaata gcgaaaggtt caatcaatgc tgccgcctgc gggtcaatac cgtctgcggg
     6781 caaaatgttc gccaccggca cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc
     6841 aatgactgaa attttttcac agcaattggt acgcccgctt ttacacgccg ggcattgctg
     6901 acaggcaaca taagggatca cagcaacttg ctgaccattt ttaagatcag caatattttt
     6961 acccagccca acaatctccc cacatatttc atggcctaaa acacgtggat aactaaaaaa
     7021 tggttgattt ccaccccagg catgaatatc ggtaccgcaa atcccgacag acttaatttt
     7081 tattaatgct tcattgtcac ccggaatagg tatctcgcgt tgtttccaga ctaattcttt
     7141 cggctgctgg caaattaaaa cattcatcgt agacatgatt cggctctcca ttttttgttg
     7201 ccttagatat caatgaaaat gaaaaatggc agcggaacgg aaaatctgtt ttgtgaaaac
     7261 acacgcataa aaatgtttta aatcgggttt taatggactc acaaggagga aaatatgagt
     7321 cgttcacaaa atttacgcca caatgtgatt aaccaggtca ttgatgatat ggcgcggggt
     7381 catatccctt ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc
     7441 accacagtgc gtcatattct cagccactta cgcgaatgcg gcgtcttgac gcaggttgga
     7501 aacgactatg ttattgcgcg taaacctgac catgatgacg gtttcgcctg tactaccgct
     7561 tcaatgagtg aacaaaacaa agtgtttgag caggcttttt tcacgatgat aaaccagcgc
     7621 cagttacgcc caggggaaac gttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc
     7681 ccggttgtag taagagaata tcttttaaaa ttcggccgtt ataatcttat tcacagcgaa
     7741 aagcgcggcc agtggagcat gaagcaattc gatcaatcct acgccgagca gctgtttgag
     7801 ctacgcgaaa tgctcgaaac acattcactt cagcactttc tcaacctgcc tgatcatgat
     7861 ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg tgacaacatc
     7921 ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt actgctttca
     7981 gctgccgaca atatcttttt tgatcaatcg cttgagatca tctcagtcat ctttcatttt
     8041 cactatcaat gggacgaaag cgatcttaaa cagcgcaata tcattgccgt cgatgagcat
     8101 atgaccattc tcagcgctct gatttgccgc agtgaccttg atgccaccct tgcgctccgt
     8161 aaccatttga attcagctaa acaatcaatg attcgatcca ttaatgagaa cacgcgttat
     8221 gctcattaag tacggattaa aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag
     8281 ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa aaatgttttt
     8341 gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atgtcaacct tgcgtaattc
     8401 acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat
     8461 caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc
     8521 agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt
     8581 attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt
     8641 aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt
     8701 ttcactcgct tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc
     8761 acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcaa tgtctggcat
     8821 ggtgcacaac tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc
     8881 gatgaaccca tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg
     8941 cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct
     9001 ggcggcgatg atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg
     9061 gatttttctc gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac
     9121 tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag
     9181 ttttgccgaa tggcgcagcc tgttccgtaa ccgtacaatg tggggaatga tgctcggatt
     9241 cagtggcatc aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac
     9301 agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg
     9361 ggctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc
     9421 tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac
     9481 gctggtcgta ccgcaagcga caacatccat gacagcggtt ctgctgattg gcatggcact
     9541 gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc
     9601 tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt
     9661 tgcgccgatc attactggtt ttattgttga taccacccac tcattccgtc tggcactaat
     9721 catctgcggt tgcgtcaccg cagcgggggc actggcgtac atcttcctgg ttcgtcagcc
     9781 gatcaacgac ccacgtaaag attaatctcc ttatgcccga taacattttg cttatcgggc
     9841 attttcatgg cgattaaaat gtttcccagt tctcctcgct atctgccacg gccattttac
     9901 gcggcgcagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgaa
     9961 tccggaacac tgccactgct tcggtcaggc gactggcctg ctcttccagc gcggcggcgg
    10021 cagcggcaga ctcttccacc agcgcggcgt tctgttgagt tacccggtcc atctcagcaa
    10081 ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa gcaatttcgc
    10141 ccataatgtc cgtcacgcgg gtcacggcgc tgacaatctc cgccattgtt tccccggcgc
    10201 tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc aggcttttaa
    10261 tttcacgagc cgcctgggcg ctgcgctggg ccagattacg cacttctccc gcgaccaccg
    10321 caaaaccgcg cccttgctca cccgcacgcg cagcctcaac cgccgcgttc aaagccagaa
    10381 tattggtctg gaaggcaatg ccgtcaatta cgctgataat atcggcgatt ttctgcgaac
    10441 tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg ccgcgttgcg
    10501 ccgtttcaga agcacttaac gccagatggc tggcctggcg cgcattttcg gcgttctgct
    10561 tcaccgttgc ggtcagttac tccatgctgg ctgccgtctc ttccagcgaa gcggcctgtt
    10621 gctcggtgcg cgaagagaga tcgttattgc cggtggcgat ttcgctggca ccgctataga
    10681 tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc tgcatatggc
    10741 gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc ggtttcacca
    10801 gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc gctaccagcg
    10861 aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc atcacgccca
    10921 ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg atatcataga
    10981 gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc tgatatccct
    11041 gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg atcagctccg
    11101 ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg gcggtgctct
    11161 gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc tgtttcagcg
    11221 aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata ttgttctgat
    11281 ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac aacgcgaccc
    11341 agctgccatt cagcgtggat tgctgctggc gaatggtttg taaaacagtg aaattttctt
    11401 tgtcattctt taaggcatta aagaacagac cgcctgatgt cagttgtaaa aggccaaaaa
    11461 cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg gtttctcttt
    11521 cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa aaggtgtaac
    11581 ccaccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta ttcattatcg
    11641 cccgggatgg cttcttgtcg ggaattgccc agagcaataa attcctctaa cagatgggtt
    11701 aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc ttcttcaatc
    11761 tgtgtctgag cgcggttata cagcgcctgc ccctctttgg ttagcgagat atagagcttc
    11821 cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat ccgcgtgagg
    11881 atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg aaaatccatt
    11941 gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa attatgccgt
    12001 ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa cgcaatggtt
    12061 agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata gaaacagtta
    12121 atcgtcacgt taccgggact gcgctgcccc tgcaattcct gattttatca aaattgatga
    12181 tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa tagtttgtta
    12241 attagatcac atttacatca cttacttttg ccaaactacg caatcgggaa taaaagcaat
    12301 cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa aggcactatc
    12361 ttcgccgtag cgttgaacca tcgcagccaa cttgatgcat ggcaggaagc gttccagcaa
    12421 tccccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg caatacggtg
    12481 attggttgcg gtgaaccgat tccctttcca cagggtgaaa aggtactgag cggtgcgact
    12541 gttgcgctga ttgtgggaaa aacggcgacg aaagtacgtg aagaagatgc ggcagagtac
    12601 atcgccggat atgcgctggc taacgacgtc agcctgccgg aagagagctt ttaccgcccg
    12661 gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt ggctctcagc
    12721 aatgtcgata atctgaccat ctataccgag atcaacgggc gtcctgccga tcactggaat
    12781 accgccgatt tacaacgtaa cgccgcgcag ttgctgagcg ccctgagcga atttgccacg
    12841 ctgaatccag gcgacgccat tctgctcggc acgccacagg cgcgcgtgga aatacagcca
    12901 ggcgatcgcg ttcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac
    12961 gaacgtgaag tgaccacgca caagagcttc ccaacgcagc cacacccgca cggcacgctg
    13021 tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa gccaccggaa
    13081 gaaccgctgg tgttcctgaa agcgccaaat accctcactg gcgataacca gacctccgtg
    13141 cgtccaaaca atattgaata catgcactat gaagcggagc tggtggtagt tattggcaaa
    13201 caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta caccgtgtgt
    13261 aacgactacg ccattcgtga ctatctggaa aactactacc gccctaacct gcgggtaaaa
    13321 agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc gatcccggac
    13381 ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca aggcaccacc
    13441 gccgatctga tcttcagcgt gcccttcctg atcgcctact taagtgaatt tatgaccctg
    13501 aatccgggcg acatgatcgc caccggcaca cccaaaggct tatctgacgt ggtgcctggc
    13561 gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag
    13621 gatacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg
    13681 actacttcca gaccaccaat ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg
    13741 gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg aaatgggcca
    13801 atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga
    13861 acgtgccaga gatcgccgcg atggaaaccg cggacaccgg cctgccgatc catcagacca
    13921 aaaatgtgtt gatcccacgc gcttcccaca actttgaatt tttcgcggaa gtctgccagc
    13981 agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg ctggtgcagc
    14041 cggtgggcgt ttgtgcgctg gtatcgccgt ggaacgtacc gtttatgacc gccacatgga
    14101 aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtact gaaaatgtcg gaactctccc
    14161 cgctgaccgc tgacagcctg ggtgagctgg cgctggaagc cgatattccg gcaggcgtgc
    14221 tgaacgtggt acagggctac ggcgcaaccg caggggatgc gctggttcgt catcatgacg
    14281 tacgtgccgt gtcgttcacc ggcggtacgg ccaccgggcg caacatcatg aaaaacgccg
    14341 ggctgaaaaa atactccatg gaactgggcg gtaaatcgcc ggtgctgatt tttgaagatg
    14401 ccgatattga acgcgcgctg gacgccgccc tgttcaccat cttctcgatc aacggcgaac
    14461 gctgcaccgc cggttcgcgc atctttattc agcaaagcat ctacccggaa ttcgttaaac
    14521 gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg
    14581 ttggcgcgct tattagccag cagcactggg aaaaagtctc cggctatatc cgtctcggca
    14641 ttgaagaagg cgcaaccctg ctggcgggcg gcccggataa accgtccgac ctgcctgcgc
    14701 acctgaaagg cggcaacttc ctgcgcccaa ccgtgctggc agacgttgat aaccgtatgc
    14761 gagtcgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag
    14821 ccgaaggctt acgtctggca aacgacgtgg agtacggcct cgcgtcgtac atctggacac
    14881 aggatgtcag caaagtgtta cgcctggcgc gtggcattga agctggcatg gtgttcgtca
    14941 acacccagaa cgtgcgtgac ctgcgccagc catttggcgg cgtaaaagcc tccggcaccg
    15001 ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac gtctgcattt
    15061 ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt agcgttagcc
    15121 gccaagatca cgcacgtacc gtcgatgtat cactctgaac tgccagggaa aaaccacggt
    15181 tgccgccagg gcgcgatcga cgggcataaa gagatcagca agcgttgccg ggaaatgggc
    15241 gtcgatacca ttatcgtttt cgatacccac tggctggtca acagtgctta tcacatcaac
    15301 tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt tatccgcgat
    15361 atgacctaca actacgaggg caacccggag ttggggcagc ttattgccga tgaagcctta
    15421 aagctcggcg tgcgggcaaa agcgcacaac attcccagcc tgaaactgga atacggcacg
    15481 ctggtgccga tgcgctacat gaatgaagac aagcacttca aagtggtctc catttcggct
    15541 ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat tctgaaagcg
    15601 atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt
    15661 attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt cgaccgccag
    15721 atggacgagc gcgtggtgaa gttgtggcgc gaaggccagt tcaaagagtt ctgcaatatg
    15781 ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac ggtgatgctg
    15841 ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttatcac cgagctattc
    15901 ccaagctctg gcaccggtca ggttaacgct gttttcccgc ttcccgcgta aggacgtttt
    15961 atgccgcact ttatcgttaa atgcagtgac aacatccgcg aagaagccga cctgccgggg
    16021 ttgttcgcca aagtgaatcc gacgctggca gccacgggta tttttccgct ggcgggtatt
    16081 cgcagccgcg tgcattgggt cgatacctgg cagatggccg acgggcagcg tgattatgcc
    16141 ttcgtgcata tgacgttgaa aatcggcgca ggtcgcagcc tggaaagccg ccagcaggcg
    16201 ggtgaaatgc tgtttgaact gattaaaacg cacttcgccg ccctgatgga gagccgcctg
    16261 ctggcgttgt cgtttgagat tgaagagctg catccgacgc tgaattttaa acaaaacaac
    16321 gtgcacgcat tgtttaagta gcgcgcagat tgcccggtgg cgctgcgctt accgggccta
    16381 caaaacccca aaccgtacac cgtaggccgg ataaggcgca gccgcatccg gcaatgccac
    16441 aggatatcgc tatgttcgat aaacacaccc acaccctgat cgcccagcgt ctggatcagg
    16501 cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga ttacccggag atcaccatcg
    16561 aagacgctta cgcggtgcag cgtgaatggg ttcgcctgaa aatcgccgaa ggtcgcacgc
    16621 tgaaaggcca caaaatcggc ctgacctcga aagcgatgca ggcaagctcg cagatcagcg
    16681 aaccggatta cggcgcgctg ctggatgaca tgttcttcca cgatggcagc gatatcccga
    16741 ccgatcgctt tatcgtgccg cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc
    16801 tgcgtgggcc aaactgcacg ctgttcgacg tctacaacgc cacggactac gtgatcccgg
    16861 cgctggagct gatcgacgct cgctgccaca acatcgatcc ggaaacccag cgcccgcgca
    16921 aagtgttcga caccatttct gataacgccg ccaacgccgg ggtgatcctc ggcggtcgtc
    16981 cgatcaaacc agacgaactg gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg
    17041 tgattgaaga aaccggcgtc gccgctggcg tgctgaatca tccggcaaac ggcgtggcct
    17101 ggctggcgaa caaactcgca ccgtatgacg tacaactgga agccgggcaa atcattctcg
    17161 gtggttcgtt cacccgcccg gttccggcgc gtaagggcga caccttccac gtcgattacg
    17221 gcaacatggg ttccattagc tgccgctttg tttaaggaga gaacgatgga aaacagcttt
    17281 aaagcggcgc tgaaagcagg ccgtccgcag atcggattat ggctggggct gagtagcagc
    17341 tacagcgcag agttactggc cggagcagga ttcgactggt tgttgatcga cggtgagcac
    17401 acgccgaata acgtgcaaac cgtgctcacc cagctacagg cgattgcgcc ctaccccagc
    17461 cagccggtag tacgtccgtc gtggaacgat ccggtgcaaa tcaaacaact gctggacgtc
    17521 ggcacacaaa ccttgctggt gccgatggta caaaacgctg acgaagcccg tgaagcggta
    17581 cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg gcagtgcgct ggcccgcgcc
    17641 tcgcgctgga atcgtattcc tgattacctg caaaaagcca acgatcaaat gtgcgtgctg
    17701 gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc agattctgga cgtggaaggc
    17761 gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg atatgggtta tgccggtaat
    17821 ccgcagcacc cggaagtaca ggccgccatt gagcaggcga tcgtgcagat ccgcgaagcg
    17881 ggcaaagcgc cggggatcct gatcgccaat gagcaactgg caaaacgcta tctggaactg
    17941 ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc tcgcccgcgc cgccgaagcg
    18001 ctggcagcac ggtttggtgc gcaggccacc gccgtgaagc ccggcgtgta ttaatgccgg
    18061 atgcgctgcg cttatccggc ctacactcgc accaaaccgt aggccggata aggcgtttac
    18121 gccgcatccg gcaaaaagcc gtaccaaatc gcggaccgga taaggcgtta cgccgcatcc
    18181 gccataaacc ttgtcgtacc ctacaaaaat cccattagag gaagaaaaat gagcgacacc
    18241 tcacctgcca taccggagag tatcgatccg gcgaatcagc ataaagcgct gactgccgga
    18301 caacaggcgg ttattaagaa gctatttcgc cgcctgagcg tctttctgtt cgtgctgttt
    18361 atcttctcgt tccttgatcg catcaacatc ggctttgccg gactcacgat gggacgcgac
    18421 ctcggtctga gcgccaccat gtttggcctc gctaccaccc tgttctatgc cgcttatgtc
    18481 atcttcggca ttcccagcaa cattatgctg agtattgtcg gtgcgcggcg ctggatcgcc
    18541 accatcatgg tgctctgggg catcgcctct actgccacca tgtttgccac tggccccacc
    18601 agcttgtacg tactgcgtat actggttggc attaccgaag ccggctttct gcctggtatt
    18661 ctgctgtatt taaccttctg gtttccggcc tatttccgcg cccgtgccaa cgccttgttt
    18721 atggtagcaa tgccggtaac gacagcgttg ggctcgatcg tttccggcta cattttgtcg
    18781 ctggatggcg tcatggcatt aaaaggctgg cagtggctgt ttttgctgga aggcttcccg
    18841 tcggtattac tcggcgtcat ggtgtggttc tggcttgatg actcaccgga caaagctaag
    18901 tggctgacga aagaagacaa aaaatgcctg caagagatga tggataacga tcgtctgacg
    18961 ctggttcagc cagagggagc catcagccac cacgccatgc aacaacgcag tatgtggcgg
    19021 gagatcttca ctccggtggt gatgatgtat accctggcgt atttctgcct gaccaacaca
    19081 cttagtgcga tcagcatctg gacaccgcag atcctgcaaa gttttaatca gggcagcagt
    19141 aatatcacca tcggcatgct ggccgccgta ccgcagattt gtaccattct cgggatggtc
    19201 tactggagcc gtcactcaga tcgccgccag gaacgaaggc atcacaccgc ccttccttat
    19261 ttgttcgctg ccgcaggttg gttactggct tcggcaactg atcacaacat gatccagatg
    19321 ctggggatca ttatggcttc gaccggatca ttcagcgcaa tggcgatttt ctggacaaca
    19381 ccggatcagt ccatcagcct gcgggcacga gcgatcggta ttgcggtgat caacgccact
    19441 ggcaacattg gttcagcgtt aagtccgttt atgatcggct ggttgaaaga tttgaccggc
    19501 agctttaaca gtggattgtg gtttgttgcc gcgctgctgg tgattggtgc ggggattatc
    19561 tgggcgattc caatgcagtc ctcccgtccg cgagcgaccc tgtaaggaac gacgatgtgt
    19621 gaccgtcaga ttgccaatat tgatatcagc aaagagtacg atgaaagcct gggcacggac
    19681 gatgtgcatt atcagtcgtt cgcccgcatg gcggcatttt ttggccgcca tatgctgcca
    19741 catcgccacg aacagtactt tcagatgcat ttcctcaata gcggacagat tgagctacag
    19801 cttgacgatc atcgctactc ggtggaagcg cccctgtttg tcctgacgcc gccgtca
//
LOCUS       DAZRVY010000069        19763 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZRVY010000069 DAZRVY010000000
VERSION     DAZRVY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.069"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHXOX_002569"
     CDS             <1..369
                     /locus_tag="ACHXOX_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR7006001.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(403..813)
                     /locus_tag="ACHXOX_002570"
     CDS             complement(403..813)
                     /locus_tag="ACHXOX_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006002.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="ACHXOX_002571"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="ACHXOX_002571"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR7006003.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHXOX_002572"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHXOX_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR7006004.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHXOX_002573"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHXOX_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR7006005.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHXOX_002574"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHXOX_002574"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR7006006.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="ACHXOX_002575"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="ACHXOX_002575"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR7006007.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHXOX_002576"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHXOX_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR7006008.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="ACHXOX_002577"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="ACHXOX_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR7006009.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="ACHXOX_002578"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="ACHXOX_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="ACHXOX_002579"
     tRNA            complement(8593..8682)
                     /locus_tag="ACHXOX_002579"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHXOX_002580"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHXOX_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR7006010.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="ACHXOX_002581"
     tRNA            9674..9749
                     /locus_tag="ACHXOX_002581"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHXOX_002582"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHXOX_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..18442)
                     /locus_tag="ACHXOX_002583"
     CDS             complement(17390..18442)
                     /locus_tag="ACHXOX_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR7006011.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18757..>19763
                     /gene="shiA"
                     /locus_tag="ACHXOX_002584"
     CDS             18757..>19763
                     /gene="shiA"
                     /locus_tag="ACHXOX_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR7006012.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5513 a         4657 c         4409 g         5184 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtctggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
    18361 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
    18421 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
    18481 gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
    18541 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    18601 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    18661 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    18721 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    18781 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
    18841 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
    18901 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
    18961 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
    19021 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    19081 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
    19141 gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
    19201 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    19261 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
    19321 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    19381 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    19441 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
    19501 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
    19561 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
    19621 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    19681 cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat cggaacgttg
    19741 agcgcatttc ctttctttat ggc
//
LOCUS       DAZRVY010000070        19530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZRVY010000070 DAZRVY010000000
VERSION     DAZRVY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.070"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHXOX_002585"
     CDS             <1..245
                     /locus_tag="ACHXOX_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR7006013.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXOX_002586"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXOX_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR7006014.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXOX_002587"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXOX_002587"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR7006015.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXOX_002588"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXOX_002588"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR7006016.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHXOX_002589"
     CDS             3423..4061
                     /locus_tag="ACHXOX_002589"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR7006017.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHXOX_002590"
     CDS             4066..4842
                     /locus_tag="ACHXOX_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR7006018.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHXOX_002591"
     CDS             4931..6295
                     /locus_tag="ACHXOX_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR7006019.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXOX_002592"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXOX_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR7006020.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXOX_002593"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXOX_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR7006021.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXOX_002594"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXOX_002594"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR7006022.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXOX_002595"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXOX_002595"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR7006023.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXOX_002596"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXOX_002596"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR7006024.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXOX_002597"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXOX_002597"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR7006025.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXOX_002598"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXOX_002598"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR7006026.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXOX_002599"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXOX_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR7006027.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHXOX_002600"
     CDS             complement(12844..13167)
                     /locus_tag="ACHXOX_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR7006028.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXOX_002601"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXOX_002601"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR7006029.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXOX_002602"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXOX_002602"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR7006030.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXOX_002603"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXOX_002603"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR7006031.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHXOX_002604"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHXOX_002604"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR7006032.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHXOX_002605"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHXOX_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR7006033.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHXOX_002606"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHXOX_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHXOX_002607"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHXOX_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR7006034.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5268 c         4896 g         4761 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgctt gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZRVY010000071        19372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZRVY010000071 DAZRVY010000000
VERSION     DAZRVY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.071"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHXOX_002608"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHXOX_002608"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR7006035.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHXOX_002609"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHXOX_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR7006036.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHXOX_002610"
     CDS             complement(3743..4162)
                     /locus_tag="ACHXOX_002610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR7006037.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHXOX_002611"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHXOX_002611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR7006038.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHXOX_002612"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHXOX_002612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR7006039.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHXOX_002613"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHXOX_002613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR7006040.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHXOX_002614"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHXOX_002614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR7006041.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHXOX_002615"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHXOX_002615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR7006042.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHXOX_002616"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHXOX_002616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR7006043.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHXOX_002617"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHXOX_002617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR7006044.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHXOX_002618"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHXOX_002618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR7006045.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHXOX_002619"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="ACHXOX_002619"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR7006046.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHXOX_002620"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="ACHXOX_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR7006047.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHXOX_002621"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="ACHXOX_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR7006048.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHXOX_002622"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="ACHXOX_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR7006049.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="ACHXOX_002623"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="ACHXOX_002623"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR7006050.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHXOX_002624"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="ACHXOX_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR7006051.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMHIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHXOX_002625"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="ACHXOX_002625"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR7006052.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4631 a         5123 c         4849 g         4769 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cttggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagagctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggctca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcctggccg atagaggccg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgcagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caaccagtac gccgagatac
    12601 gcctgagaac gcgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccgaccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgtggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atggagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcgt tagaggtcag cccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgat gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcgt acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacgaccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactaccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    14161 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
    14761 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcagcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtttgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
    16741 agcaatgtgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    17641 gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
    18241 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
    18361 tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gg
//
LOCUS       DAZRVY010000072        19161 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZRVY010000072 DAZRVY010000000
VERSION     DAZRVY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.072"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHXOX_002626"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHXOX_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
     gene            811..1443
                     /locus_tag="ACHXOX_002627"
                     /pseudo
     CDS             811..1443
                     /locus_tag="ACHXOX_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1546..2048
                     /locus_tag="ACHXOX_002628"
                     /pseudo
     CDS             1546..2048
                     /locus_tag="ACHXOX_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="ACHXOX_002629"
     CDS             complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="ACHXOX_002629"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR7006053.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="ACHXOX_002630"
     CDS             complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="ACHXOX_002630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR7006054.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="ACHXOX_002631"
     CDS             complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="ACHXOX_002631"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR7006055.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="ACHXOX_002632"
     CDS             complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="ACHXOX_002632"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR7006056.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="ACHXOX_002633"
     CDS             complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="ACHXOX_002633"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR7006057.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="ACHXOX_002634"
     CDS             complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="ACHXOX_002634"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR7006058.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="ACHXOX_002635"
     CDS             complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="ACHXOX_002635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR7006059.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="ACHXOX_002636"
     CDS             complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="ACHXOX_002636"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR7006060.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="ACHXOX_002637"
     CDS             complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="ACHXOX_002637"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR7006061.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="ACHXOX_002638"
     CDS             complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="ACHXOX_002638"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR7006062.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="ACHXOX_002639"
     CDS             complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="ACHXOX_002639"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR7006063.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="ACHXOX_002640"
     CDS             complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="ACHXOX_002640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR7006064.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="ACHXOX_002641"
     CDS             complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="ACHXOX_002641"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR7006065.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="ACHXOX_002642"
     CDS             complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="ACHXOX_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR7006066.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4864 a         5237 c         4860 g         4200 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttctgcccc ctgtcaactc aatctgccaa taaataacat
      661 aattatattt ttccttatat gaattccttt ttaaaatagc gaagccactg taaaagatac
      721 tttttgtata gatctaacaa ccgtaataaa acaaacactc ataactcatt ataaataatg
      781 ataaaataac gtcttttatt gataatttat atatggaaaa aaattccttt tgttatattg
      841 tttctgattt cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa
      901 cgccccgaca cgtcagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg
      961 tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag tatataaaag
     1021 agttctttgt cagtagcggt gccagagtta ccgcgcagag cgtccccgat tggctattac
     1081 gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt tatcccgatc
     1141 ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc gtcaggtaaa
     1201 aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc catggtttat
     1261 tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc ccgccgtaat
     1321 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg atacagcaga
     1381 cagattgaat tatcagaaaa tggctcagat ggtgttataa ccttgttaca caacagtaaa
     1441 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1501 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggt
     1561 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1621 ttgatggata atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc
     1681 tgggattttt tacactttgg gctgacaggc acccctgctt ttgatcccgc gaagaatgat
     1741 ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga tggcttcctg
     1801 ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga
     1861 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1921 gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct gatatggaaa
     1981 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     2041 ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact
     2101 tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat cagcggttgt
     2161 ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat accgcctgcg
     2221 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg caggtacagg
     2281 ctcaccgcca cgcgcaggta gtagtaaagg ccgcttgccg aaccgacaac cacggcgccc
     2341 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accaataaag
     2401 cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg
     2461 atcgggcgat gccagaacag tccgcggtaa gagtacaggg aatcagcatc cgggccacga
     2521 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctaaa cagataaccg
     2581 gccaggtaaa cccctaccgc ttccatcgac atctcgccag tttgcagcgc aatcagcgcc
     2641 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     2701 tggctcagcg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc
     2761 acgcgaatcg cttcgctgtc acccatcggt gcgtagagga acagacgcat caccacaccg
     2821 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2881 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2941 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccaaggttt
     3001 ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccaaac
     3061 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagtgaa
     3121 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     3181 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     3241 attaacacca acaggtagaa ctcgtccttg ttgtcgttat agccttcaag ccacgggtag
     3301 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3361 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcctg cctggccgac aaaccagagc
     3421 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3481 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3541 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3601 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3661 tgcggataga agccctgcag taccagcagc accaccagca acaggatcat aaacagctca
     3721 cgcagcgaca tccctggcag ttcctggctg gcaatctcgc ttttcgcttt accgaagtaa
     3781 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3841 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3901 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3961 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     4021 taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     4081 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     4141 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc
     4201 cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc
     4261 gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     4321 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4381 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     4441 aacaggtatt ccgcaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4501 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     4561 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4621 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4681 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4741 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4801 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     4861 atggcgagat gaatagagat acccaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4921 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4981 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     5041 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     5101 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     5161 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc
     5221 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     5281 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     5341 cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg
     5401 aatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5461 cctgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtact
     5521 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     5581 cagccaagct gccagcagaa tgccgacaat cgcgaccacg ccagaggtaa tttccagggt
     5641 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5701 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5761 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5821 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5881 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5941 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     6001 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     6061 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     6121 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     6181 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     6241 cagcgtaacc gccccgacaa tgcccaccag atgcagaatt tccggcgtca tcaggaacag
     6301 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atggttgcgg cgtggatcag
     6361 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     6421 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6481 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6541 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6601 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6661 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6721 caccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6781 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6841 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6901 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcaccgt tagcgaagaa
     6961 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     7021 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     7081 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     7141 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     7201 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     7261 cttccggcga caacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     7321 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaatgaa taagattgcc
     7381 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7441 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7501 ccacaaccag acctgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7561 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7621 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac
     7681 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7741 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7801 ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg
     7861 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7921 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7981 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     8041 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     8101 gatcagcaga tcctcttttt cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     8161 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     8221 gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     8281 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     8341 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8401 atagatcggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8461 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8521 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8581 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     8641 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8701 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8761 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8821 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8881 tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8941 ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     9001 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     9061 gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc
     9121 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     9181 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     9241 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     9301 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9361 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9421 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9481 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9541 cgccagccag caccggagca ataccggaca tacccatcgg caagcccacc tgccctgccg
     9601 tcagcccttc agcgatttca accggcagcg tgaccgtatt gccatcgtaa ctaaaggaga
     9661 cgtgtgtacc tgcgttcaca cctaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9721 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9781 ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9841 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9901 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9961 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca
    10021 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
    10081 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
    10141 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg
    10201 ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg
    10261 tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat
    10321 aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac
    10381 cgtcgctttc agcaaaactg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg
    10441 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10501 tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg
    10561 tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10621 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10681 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10741 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10801 caggttcgat accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac
    10861 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10921 gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10981 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    11041 tcacagcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    11101 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    11161 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    11221 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    11281 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    11341 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11401 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11461 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11521 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11581 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11641 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11701 tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11761 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11821 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacag
    11881 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11941 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    12001 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    12061 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    12121 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    12181 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    12241 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    12301 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12361 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12421 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12481 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12541 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12601 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12661 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12721 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12781 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12841 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12901 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12961 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    13021 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    13081 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    13141 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    13201 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    13261 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    13321 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13381 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc tccgccgcgc
    13441 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13501 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13561 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13621 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13681 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13741 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13801 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13861 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13921 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13981 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    14041 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    14101 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    14161 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    14221 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    14281 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    14341 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14401 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14461 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14521 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14581 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14641 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14701 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14761 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14821 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14881 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14941 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    15001 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    15061 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    15121 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    15181 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    15241 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    15301 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15361 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    15421 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15481 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15541 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15601 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15661 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15721 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15781 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15841 ttcgccaatc accggatcat caagatgatc acgggtctgc caggcgggtt cttgcgcggt
    15901 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15961 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    16021 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    16081 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    16141 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    16201 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    16261 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    16321 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16381 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16441 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16501 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16561 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16621 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16681 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16741 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16801 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16861 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16921 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16981 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    17041 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    17101 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    17161 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    17221 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    17281 cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat
    17341 caacaccatt agcatttaat gccctttcac gccagatcct tgacgactcc aggataatta
    17401 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17461 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17521 gtcattccat aacaaaaacg cagcaacaaa tttacgtatt tttaacatca ttgtagcagg
    17581 tgattttttt caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat
    17641 gaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg
    17701 gggtttaaaa cacccccagc ggctcgtttt tacactattg tctcaggaat tatctatcgt
    17761 ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagaca
    17821 ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca
    17881 gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta
    17941 agccatctac tccactcagc acacgcaggt ccgggctcat catttcaatc ggccttgccg
    18001 tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag
    18061 ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg cgaaacggac
    18121 ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg tattccgctg
    18181 cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat
    18241 gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata taggcattac
    18301 gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga
    18361 aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat
    18421 tactgaacat taatgatgag caggcctcat cattaaaacg cagtattttc ctggcgtaac
    18481 caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt
    18541 ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac
    18601 acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca
    18661 gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac
    18721 tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta
    18781 atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg
    18841 cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat
    18901 cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt
    18961 ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc
    19021 gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg
    19081 agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc tgatgcaaag
    19141 ttatgttatg ttaggcaaag t
//
LOCUS       DAZRVY010000073        18983 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZRVY010000073 DAZRVY010000000
VERSION     DAZRVY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.073"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(204..2195)
                     /gene="tkt"
                     /locus_tag="ACHXOX_002643"
     CDS             complement(204..2195)
                     /gene="tkt"
                     /locus_tag="ACHXOX_002643"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR7006067.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2473..3231
                     /gene="loiP"
                     /locus_tag="ACHXOX_002644"
                     /gene_synonym="yggG"
     CDS             2473..3231
                     /gene="loiP"
                     /locus_tag="ACHXOX_002644"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR7006068.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3374..4294)
                     /gene="speB"
                     /locus_tag="ACHXOX_002645"
     CDS             complement(3374..4294)
                     /gene="speB"
                     /locus_tag="ACHXOX_002645"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR7006069.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4432..6408)
                     /gene="speA"
                     /locus_tag="ACHXOX_002646"
     CDS             complement(4432..6408)
                     /gene="speA"
                     /locus_tag="ACHXOX_002646"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR7006070.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6417..6548)
                     /gene="yqgB"
                     /locus_tag="ACHXOX_002647"
     CDS             complement(6417..6548)
                     /gene="yqgB"
                     /locus_tag="ACHXOX_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR7006071.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6642..6785)
                     /gene="yqgG"
                     /locus_tag="ACHXOX_002648"
     CDS             complement(6642..6785)
                     /gene="yqgG"
                     /locus_tag="ACHXOX_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR7006072.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6896..7147)
                     /gene="yqgD"
                     /locus_tag="ACHXOX_002649"
     CDS             complement(6896..7147)
                     /gene="yqgD"
                     /locus_tag="ACHXOX_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR7006073.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7203..8357
                     /gene="metK"
                     /locus_tag="ACHXOX_002650"
     CDS             7203..8357
                     /gene="metK"
                     /locus_tag="ACHXOX_002650"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR7006074.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            8793..10187
                     /gene="galP"
                     /locus_tag="ACHXOX_002651"
     CDS             8793..10187
                     /gene="galP"
                     /locus_tag="ACHXOX_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR7006075.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            10264..10761
                     /locus_tag="ACHXOX_002652"
     CDS             10264..10761
                     /locus_tag="ACHXOX_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR7006076.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            10856..11563
                     /gene="endA"
                     /locus_tag="ACHXOX_002653"
     CDS             10856..11563
                     /gene="endA"
                     /locus_tag="ACHXOX_002653"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR7006077.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            11643..12374
                     /gene="rsmE"
                     /locus_tag="ACHXOX_002654"
     CDS             11643..12374
                     /gene="rsmE"
                     /locus_tag="ACHXOX_002654"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR7006078.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            12387..13337
                     /gene="gshB"
                     /locus_tag="ACHXOX_002655"
     CDS             12387..13337
                     /gene="gshB"
                     /locus_tag="ACHXOX_002655"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR7006079.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            13446..14009
                     /locus_tag="ACHXOX_002656"
     CDS             13446..14009
                     /locus_tag="ACHXOX_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR7006080.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14009..14425
                     /gene="ruvX"
                     /locus_tag="ACHXOX_002657"
     CDS             14009..14425
                     /gene="ruvX"
                     /locus_tag="ACHXOX_002657"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR7006081.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(14600..15580)
                     /locus_tag="ACHXOX_002658"
     CDS             complement(14600..15580)
                     /locus_tag="ACHXOX_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR7006082.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            15598..16302
                     /gene="yggS"
                     /locus_tag="ACHXOX_002659"
     CDS             15598..16302
                     /gene="yggS"
                     /locus_tag="ACHXOX_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR7006083.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            16320..16886
                     /gene="yggT"
                     /locus_tag="ACHXOX_002660"
     CDS             16320..16886
                     /gene="yggT"
                     /locus_tag="ACHXOX_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR7006084.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            16883..17173
                     /gene="yggU"
                     /locus_tag="ACHXOX_002661"
     CDS             16883..17173
                     /gene="yggU"
                     /locus_tag="ACHXOX_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR7006085.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17181..17774
                     /locus_tag="ACHXOX_002662"
     CDS             17181..17774
                     /locus_tag="ACHXOX_002662"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR7006086.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            17767..18903
                     /gene="hemW"
                     /locus_tag="ACHXOX_002663"
     CDS             17767..18903
                     /gene="hemW"
                     /locus_tag="ACHXOX_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR7006087.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         4538 a         5039 c         4938 g         4468 t
ORIGIN      
        1 ttattaattt cttatataac attgagttat agatataaca acaaatgtca atacgcatat
       61 cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat ctacaacacg
      121 ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg tcgccgaagc
      181 gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac gacgttgtcg
      241 acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc gaaggtggtc
      301 ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc aatacccgct
      361 tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta agccgcatcc
      421 tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac gccttcggca
      481 gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc gatgaagatc
      541 agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat gtttgccagt
      601 tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag cgcggtcgga
      661 ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg gtcacacgga
      721 cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac cggctggtga
      781 gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac ctgacgctgt
      841 ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat caggaaggtg
      901 gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc ggtcataccg
      961 aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat tgctttagaa
     1021 ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag gaattccggc
     1081 aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc gattttcgcc
     1141 ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc agacggcatt
     1201 tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc
     1261 gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc atcccactgc
     1321 gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg ttcgcgggtc
     1381 agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc
     1441 ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc agtcactgcg
     1501 cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat gtcgcgaata
     1561 acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg
     1621 tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc
     1681 gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg
     1741 aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg cgccgccagc
     1801 gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag cggaccggtg
     1861 gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt agagtgcagc
     1921 tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg cagcaggctg
     1981 tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc agcccaggac
     2041 ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat gtcagccata
     2101 cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat gctcagcgca
     2161 cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg gatgatgaag
     2221 ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa atttgccaac
     2281 aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt cagaaatctc
     2341 tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt tccttatact
     2401 gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct gaccttgtgc
     2461 ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg
     2521 actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga agcttttcag
     2581 gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg tcaggagatg
     2641 gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct gacaactatt
     2701 gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt gtatatggcg
     2761 aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag cgggctgatg
     2821 gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg
     2881 ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt gcgagtagct
     2941 gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg tgatctgggc
     3001 gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga tgattattct
     3061 tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa
     3121 ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc ggcatccgcc
     3181 gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta aagcctggtg
     3241 gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg gcacatttgt
     3301 taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct acgggtggca
     3361 tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc tccagcgcca
     3421 gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc acttccacta
     3481 cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata gcgcgatcgg
     3541 aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga tccaggcagt
     3601 cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact tgggcgataa
     3661 cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag ccgttgtctt
     3721 tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc agaccttctt
     3781 tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca taggtatcgg
     3841 tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga gcacgcagca
     3901 gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg gcagccagca
     3961 gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca aaggcatata
     4021 ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc cacgggaagc
     4081 ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga ccgtggcgac
     4141 cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc cagtctgcat
     4201 cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca ttggaaacca
     4261 gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta caaagtcgat
     4321 ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt gtcacaataa
     4381 attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga cttactcatc
     4441 ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact gttgttgcag
     4501 ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca gcgttttcgg
     4561 atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag acagttctac
     4621 ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac cgaacaggtt
     4681 gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga gcatcggcgg
     4741 attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac catcaatata
     4801 gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc gacgttccgg
     4861 cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc cccatgcgtc
     4921 cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac gttcctgcag
     4981 ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct gcacttcatg
     5041 gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa aggtgccgga
     5101 agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc attcacgcaa
     5161 agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc tttgcagcgc
     5221 gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt ccacgccgat
     5281 gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt cggtgattac
     5341 cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga tgatgttgtt
     5401 ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg tgccttcata
     5461 gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag
     5521 ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa tattcgccat
     5581 ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg cttcacgcag
     5641 ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt tttccccgcc
     5701 ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc ccagacgagg
     5761 aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca tcttctcaat
     5821 gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga tatattcgcg
     5881 gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg ccagtactgc
     5941 catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat gaatcaggga
     6001 ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt aatcgccgtt
     6061 atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca aacggtgctg
     6121 caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt cacgagtttt
     6181 cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc acacgctgat
     6241 gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat tgtaagtacg
     6301 cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc gtagtacacc
     6361 gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag cgaacctcaa
     6421 attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg cgataacaac
     6481 ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc
     6541 tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg gcgaaaccac
     6601 gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact gtaaccagct
     6661 cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa agcagagatg
     6721 cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag cagacaacgg
     6781 ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa gccaaaactc
     6841 tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag
     6901 ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa atacgcagaa
     6961 tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga cacgattcaa
     7021 aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg ttaatccatc
     7081 cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg gtaggatccg
     7141 ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag gtgatattaa
     7201 atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct gacaaaattg
     7261 ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg aaagcacgcg
     7321 ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa atcaccacca
     7381 gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc
     7441 attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc ggcaaacagt
     7501 ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc
     7561 agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca gcacctatca
     7621 cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt
     7681 ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc aaaatcgttg
     7741 gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag aaatcgctgc
     7801 aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg ctgacttctg
     7861 ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg atgggtgact
     7921 gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca cgtcacggtg
     7981 gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc tacgcagcac
     8041 gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa attcaggttt
     8101 cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc ggtactgaga
     8161 aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg cgcccatacg
     8221 gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca gcatacggtc
     8281 actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg
     8341 ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg cgctggcctt
     8401 tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa aagctgcccg
     8461 cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac tactgaaacc
     8521 gattacacca ataacaccag ccaaatattt gtaatatttt catattatta ttcagttttc
     8581 acagttgtta catttctttt cagtaaagtc ttaattgcag ataacagcgt ttaatctatg
     8641 atgatataac tcaattattt tcatgcactt aaaccataac taagataaat gttagtgtaa
     8701 gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc tatcttaatt
     8761 cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa caggggcggt
     8821 caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga ttactctttg
     8881 gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa ttccagatta
     8941 cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca gtcggtgcgg
     9001 tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg atcggcgcaa
     9061 ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa gtactgattc
     9121 tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca ccgctgtacc
     9181 tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat cagttgatga
     9241 tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac accggtgcat
     9301 ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt ggtgtcttct
     9361 tcctgccaga cagcccacgt tggtttgccg ccaaacgccg ttttgttgat gccgaacgcg
     9421 tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat gaaatccgtg
     9481 aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc aacttccgcc
     9541 gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg atgaacgtca
     9601 tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc acagagcaaa
     9661 tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc gcaatcggcc
     9721 ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg atggctgctg
     9781 gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg gcgcagtatt
     9841 tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc ggtccgctga
     9901 tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc atcacctgct
     9961 ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg accatgctca
    10021 acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta ctgtttatcc
    10081 tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat attgaacgta
    10141 acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct cctcaggctt
    10201 cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct atcctctgcc
    10261 gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg cggcctgcgg
    10321 gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc aaaactctct
    10381 tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga aatccgcctc
    10441 aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt accgcacgaa
    10501 ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg caaagagtgg
    10561 aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca gttcgaactg
    10621 caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca tcagcttacc
    10681 gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt tcactgcggt
    10741 gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat ctggctgatt
    10801 gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt aagtgatgta
    10861 ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc cggcgttggc
    10921 cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc acgctgacgc
    10981 gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag gcgttgttga
    11041 tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc gcgtagagtg
    11101 ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc aggacggtgg
    11161 acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata tgcataacct
    11221 gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca gccagtggaa
    11281 tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag aaaaagctgc
    11341 cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata tgcgcgacca
    11401 atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga acaagatgta
    11461 tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc aaggcaatca
    11521 taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac tagcgggatt
    11581 ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc ggatttttaa
    11641 ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct cacatcgcgc
    11701 tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg gggcaggcgt
    11761 tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc gccagcaaaa
    11821 aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct ccgctgcata
    11881 ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc cagaaatcga
    11941 tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc gttaaactgg
    12001 atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt gctgcctgtg
    12061 agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg gaagcctggt
    12121 gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt aacagcatca
    12181 atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa ggcggtttat
    12241 cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg ttgggacctc
    12301 gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa gtacgatttg
    12361 gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg gaccccatcg
    12421 caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca cagcgtcgtg
    12481 gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc gaagcccgcg
    12541 cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg ttcgtcggtg
    12601 aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac ccgccgtttg
    12661 ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa gggacgctga
    12721 tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc gcctggttct
    12781 ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa gcgttctggg
    12841 agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg tcgattttcc
    12901 gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact gagcatggca
    12961 ctcgctactg catggcacaa aattacctgc cagccattaa agatggcgac aaacgcgtgc
    13021 tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag gggggcgaaa
    13081 cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg gaaagtgact
    13141 ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt tttgttggtc
    13201 tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg
    13261 agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac
    13321 gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc tgagtgacag
    13381 cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa acagaacctc
    13441 tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag gatccgattt
    13501 tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg gggatcatcg
    13561 tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg aagatcacgc
    13621 cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc ggtccgctgg
    13681 ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc agcattcgca
    13741 tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc ggcaccgata
    13801 aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa ggtcaactgg
    13861 aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat attctgttca
    13921 aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg gatattctca
    13981 ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct tcgacttcgg
    14041 caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc gccctttgcc
    14101 tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt tactgaaaga
    14161 gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca ccgagcaacc
    14221 attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt
    14281 aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt ttgaacaggg
    14341 cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta ttattctcga
    14401 aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag tcgttaccgc
    14461 catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg attgtaggtc
    14521 tgataagacg cgcaaacgtc tcatcagacg tttattgcct ttgtaggccc gataagcttg
    14581 cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga aaactctgct
    14641 gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac tggtgggttt
    14701 tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca aacagcgcca
    14761 cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt aaactacctg
    14821 ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc agtcgctcaa
    14881 ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg gtttctgccg
    14941 ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa atcacatcag
    15001 gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc aaaccaattt
    15061 cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga tcttccagcg
    15121 tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc agcgtggtag
    15181 atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc ttgagtaatt
    15241 ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa ggtaacagcc
    15301 gtaacgccag cgaaatgcca tgccgttgcg caaatgcgct gccacgcaat cgctggtttt
    15361 ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc cgctgatcgt
    15421 catccagcca ctcccgcagt agctcttcga cgtccggcgc ctcaaacggc gcagcttcca
    15481 ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga tccgagacgt
    15541 tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc tcggaaaatg
    15601 aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc tgcaacgcgt
    15661 tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa acctgcgagc
    15721 gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta cgttcaggaa
    15781 ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga atggcatttt
    15841 attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga ctggtgtcat
    15901 accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc agaacttccc
    15961 cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc cgggattcaa
    16021 ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt acgtctgcgc
    16081 gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga agttgcacgc
    16141 caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga cacgctctct
    16201 ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat ggttcgtatc
    16261 ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa aggaacgcca
    16321 tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg gtgctgttat
    16381 tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca cagtttgtag
    16441 tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca atgggcccca
    16501 ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc atcgtgctgt
    16561 ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt ctgctgaaaa
    16621 ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc tgggtaagcc
    16681 agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg ctgcgcccga
    16741 ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc ctcgttctgc
    16801 tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga aacatgctgc
    16861 tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg gtctggtttt
    16921 acggctctat attcagccga aagccagccg tgattctatt gtcggtttac atggcgacga
    16981 agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc atctggtgaa
    17041 gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa aaggcgaact
    17101 tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag aaatcgcggc
    17161 gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat gccggtaaag
    17221 tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc caaacagacc
    17281 tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg attctgaaag
    17341 cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct ggtctggcgg
    17401 tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt gaagacgcga
    17461 ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg gacgaccaac
    17521 gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat ccgactccgc
    17581 tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc actggtggct
    17641 ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc gaactgaccc
    17701 gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg ctggacgctt
    17761 tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg gtgcgtgcag
    17821 aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc gcacgacgat
    17881 tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca gggccgtgaa
    17941 gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc ggcgatgcaa
    18001 acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga aattactatg
    18061 gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg tgctggtgtg
    18121 aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg acttgggcgt
    18181 attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct ggggctgcgt
    18241 agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga ggcgcttggc
    18301 gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca actgaccatc
    18361 gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgatga cgcgctgtgg
    18421 gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca atatgaaact
    18481 tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg gcgatttggt
    18541 gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga tgggcgcatt
    18601 ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta tctggaaagc
    18661 cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg cttccgtctg
    18721 ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga agatgtgatt
    18781 cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc ggattactgg
    18841 cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt cctcgctgag
    18901 taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt aattaacttc
    18961 ggcaaaatat atttcttact gaa
//
LOCUS       DAZRVY010000074        18846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZRVY010000074 DAZRVY010000000
VERSION     DAZRVY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.074"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..104)
                     /locus_tag="ACHXOX_002664"
     CDS             complement(<1..104)
                     /locus_tag="ACHXOX_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006088.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAV"
     gene            complement(255..2126)
                     /gene="ppiD"
                     /locus_tag="ACHXOX_002665"
     CDS             complement(255..2126)
                     /gene="ppiD"
                     /locus_tag="ACHXOX_002665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR7006089.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2318..2608)
                     /gene="hupB"
                     /locus_tag="ACHXOX_002666"
     CDS             complement(2318..2608)
                     /gene="hupB"
                     /locus_tag="ACHXOX_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR7006090.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2799..5153)
                     /gene="lon"
                     /locus_tag="ACHXOX_002667"
     CDS             complement(2799..5153)
                     /gene="lon"
                     /locus_tag="ACHXOX_002667"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR7006091.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5341..6615)
                     /gene="clpX"
                     /locus_tag="ACHXOX_002668"
     CDS             complement(5341..6615)
                     /gene="clpX"
                     /locus_tag="ACHXOX_002668"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR7006092.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6741..7364)
                     /gene="clpP"
                     /locus_tag="ACHXOX_002669"
     CDS             complement(6741..7364)
                     /gene="clpP"
                     /locus_tag="ACHXOX_002669"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR7006093.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7610..8908)
                     /gene="tig"
                     /locus_tag="ACHXOX_002670"
     CDS             complement(7610..8908)
                     /gene="tig"
                     /locus_tag="ACHXOX_002670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR7006094.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9252..9569)
                     /gene="bolA"
                     /locus_tag="ACHXOX_002671"
     CDS             complement(9252..9569)
                     /gene="bolA"
                     /locus_tag="ACHXOX_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR7006095.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9874..10452
                     /locus_tag="ACHXOX_002672"
     CDS             9874..10452
                     /locus_tag="ACHXOX_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7006096.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10496..11971
                     /gene="ampG"
                     /locus_tag="ACHXOX_002673"
     CDS             10496..11971
                     /gene="ampG"
                     /locus_tag="ACHXOX_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR7006097.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12433..13380
                     /gene="cyoA"
                     /locus_tag="ACHXOX_002674"
     CDS             12433..13380
                     /gene="cyoA"
                     /locus_tag="ACHXOX_002674"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR7006098.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13402..15393
                     /gene="cyoB"
                     /locus_tag="ACHXOX_002675"
     CDS             13402..15393
                     /gene="cyoB"
                     /locus_tag="ACHXOX_002675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR7006099.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15383..15997
                     /locus_tag="ACHXOX_002676"
     CDS             15383..15997
                     /locus_tag="ACHXOX_002676"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR7006100.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15997..16326
                     /locus_tag="ACHXOX_002677"
     CDS             15997..16326
                     /locus_tag="ACHXOX_002677"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR7006101.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16338..17228
                     /gene="cyoE"
                     /locus_tag="ACHXOX_002678"
     CDS             16338..17228
                     /gene="cyoE"
                     /locus_tag="ACHXOX_002678"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR7006102.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17413..17724
                     /locus_tag="ACHXOX_002679"
     CDS             17413..17724
                     /locus_tag="ACHXOX_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR7006103.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17708..18250
                     /locus_tag="ACHXOX_002680"
     CDS             17708..18250
                     /locus_tag="ACHXOX_002680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7006104.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18306..>18846)
                     /locus_tag="ACHXOX_002681"
     CDS             complement(18306..>18846)
                     /locus_tag="ACHXOX_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR7006105.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4215 a         4958 c         4581 g         5092 t
ORIGIN      
        1 accgccgtcg gtttcgccac agaagctgcc gccagcgcgc tatgagacat tccggcacag
       61 gccagggaca gggtaatgag cagtgcttta attccgtgtt tcatactgtt ttctccttgt
      121 ttgttaacag tggaatcacc ttaacgggag tgaaaacagt aaacaaatgg caatgttcag
      181 aaatggaaaa ggccgcgaaa gcgacctttt cttgttacag ctgcaacgca tttttttgcg
      241 aggcggcttc aggattattg ctgttccagc gcatcgccaa ttttgatttt cgcctcttta
      301 cgcaggttac tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt gataccctgc
      361 accatcgctt ttttctgatc ttccggcatt gaaccttgtt tcacttcatc cagcgccagc
      421 agaaccacat taccttgcat atcggtcgcc ataccgtagc tcggtttgtc tttcgctggc
      481 agtggcagtg caaacgccgc ctggctaatc gggtcacgac cggaacggct taaggttttc
      541 ggctcgccaa atttcagacc ggcagcctgc atagcttccg cccctttgcc ggctttcaaa
      601 tcaaccagca gtttctcagc atccactttc gcctgttgtt cagctttgtt gtgctgaacc
      661 agtgccttaa cttgttcctg aacatctgcc aacggtttca ccgcttccgg tttgtgctcg
      721 ctgatgcgca gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga gttgatgccc
      781 ggcgcgccgt tttcacctac cagaccgccg ttaaagatag cgtcggcaac cggcttgaag
      841 ttcaactctt ccggcaggtt atctttgctg aaccaacccg tctgagtggc tttaacgccg
      901 gcagcttgct ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc gctcactttc
      961 tgctgcagcg catagtacgc atcgagggct ttttcgtgtt tcactttcgc cgcaatgtcg
     1021 tcacgtactt cgtctaacga tttcactttc gctggctgaa tgtcgtccag acgtacaatc
     1081 aggaaaccga ccgaagattt gatgacacca gacagttggc ctttttcttt cagaccagca
     1141 tttttcagtt cgtcggggat agtggcatct tctaaccaac ccatatcgcc gccgttacgg
     1201 gcagagataa tatcggcaga tttttctttg gctaatgcag caaaatcacc gcctttattc
     1261 agctcatcaa gtaccgcttt cgcttcatct tcagttttgg tctggatgat gctgtagcgg
     1321 gtacgctgcg gctgggtgaa ttgatcctga tgctgatcgt agtagctctg gatatccgca
     1381 tcgctaaccg gttgctgcat cgttgcggca tccagcttga tgtaactcac gcggaattgt
     1441 tccggcgtca tgaaattgtt tttgttttgt tcgtagtagc tggcaatctc ctgttcggtc
     1501 acaggctgct tcgccgccag cgcgttaaca tcgatagtcg cctcacgcac cacgcgttgt
     1561 tgcgcgacca gtgccgccag ctcgtcggtt tcacctttca gcataaaatc ggtaccggca
     1621 acaccgttaa tcagctgttg ggtagtgagc tggttacgca gcgcctgggc gtactgatcg
     1681 gcggtcatcc ccatctggtt gaggataccg ttatagcggc tgttatcaaa tttgccgtca
     1741 acctggaagg ctggggtcgc gaaaatcgcc tgtttaacct gctcatcact gatacccagt
     1801 ttcagctcac gagcgtactg atccaacagc gcctcgtcga tcagacgatt cagcacctgt
     1861 tgacgcaggg ttttcatata gccttcgttc gctgccagct cggagtattg atcgcccagc
     1921 tgttgctgca tgcgattacg ctcgctgtta aaggcgttct cgaactgccc acggctgatt
     1981 tcctggtcat tcacttttgc ggcgtagtta ttgcctccgc caatcaggta accactcacg
     2041 ccggtcaata tgaacgacac gataatgata ccgaaaataa tcttgagcac gagactgttt
     2101 gcagccgtgc gtaagctgtc catcatggtg taacaacact ccgctgtaga tgactgtgta
     2161 cctcacgcaa cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg gggcaattgt
     2221 cagcccacaa gacacagaaa gtcaccgaat acaaataaaa aaggcacatc agtagatgcg
     2281 cccttgaact tcgtcacatc cccactgggg acaacgctta gtttaccgcg tctttcagtg
     2341 ctttacctgc acggaagctc ggtactttag cggcagcgat ggtgatctct ttaccggtct
     2401 gcgggttgcg gccagtacgg gcagcacgct ctttaacggc aaaagtacca aaacctacca
     2461 gtgctacatc atccccttct ttcagagatt cagttacgga agcaataata gcatctaacg
     2521 cacggccagc cgcagcttta gagatatcag cccctgcagc aatcttgtcg atcaattgag
     2581 atttattcac tcttctcttc ctctttataa tttatatcgc acttgaatcc ttcaaggtac
     2641 gaacgcgcag cagttatatc aggccagcca tccccttaca acacccctta ataagggcaa
     2701 gcccgatccg ccatctaact tagcgagaca aaaaaaggct ggcaagcccg aattagcctg
     2761 ccagccctgt ttttattagt gcattttgcg cgaggtcact attttgcagt cacaacctgc
     2821 atgccagacg gttcattttg cagcgccaga gtcagaactt cctcaatgcg cttcacagga
     2881 tgaatgtcca gatcggcaat tacgttgtca ggaatctctt ccagatcgcg tttattttcg
     2941 aacggaatta gcactgtttt aatcccgccg cgatgcgctg ccaggagttt ttctttcaaa
     3001 ccaccgatcg gcagtacctg accacgcaga gtgatctcac cggtcattgc cacatcggca
     3061 cgaaccgggt taccggtcag gcaagaaacc agcgcggtgc acatagcaat accggcactc
     3121 ggaccatctt tcggcgtcgc accttccggt acgtggacgt ggatgtcacg tttttcgtaa
     3181 aaatcagggt tgatccccag tttttccgca cgcgcacgaa ccaccgttaa cgccgcctga
     3241 atagactcct gcatcacttc gccgagcgaa ccggtatagg tcagtttgcc tttacccgga
     3301 acacatgcgg tttcaatggt cagcaagtca ccgcccactt ccgtccacgc cagaccggtt
     3361 acctgaccga cacggttttc gttatcagcg cgaccatagt cgaaacgctg aacaccgagg
     3421 tagtcatgca ggttatcgcc gttaatttcg atatgtttta atgacttatc gagcagtaac
     3481 tgcttaaccg ctttgcggca cagtttggag atttcacgct ccagaccacg cacgcccgcc
     3541 tcacgggtgt agtaacgaat aatgccgata atggcgctat cgtcgacggt cagctcacct
     3601 tttttcagtg cattacgttc aatctgcttc ggcagcaggt gacgtttggc gatgttcagt
     3661 ttttcatctt cggtataacc ggagaggcga atcacttcca tacgatccag cagcggtgcc
     3721 ggaatgttca tggagttcga cgtcgcgaca aacatcacgt cgctgagatc gtaatccact
     3781 tccaggtagt ggtcgctgaa cgctacgttc tgctctggat ccagcacttc aagcagtgca
     3841 gaggccggat cgcctcgcat gtcagaagac attttgtcga tctcatcgag caggaacagc
     3901 gggtttttca cgcccacttt cgccattttc tggatcagtt tacccggcat agaaccgatg
     3961 taagtacggc ggtgaccacg gatttccgct tcatcacgca cgccgcccag cgccatacgg
     4021 acatatttac gcccggtggc tttggcaatg gactgaccaa gagaggtttt acctaccccc
     4081 ggcggcccta ccaggcagag gatcggtccc ttgattttgt tgacacggct ttgaaccgca
     4141 aggtactcaa ggatgcgatc tttcacgcgc tccagaccat aatggtcggt atcaaggatt
     4201 tcctgcgcct gacgcaggtc ttttttgacc ttgctgcgcg cattccacgg tacctgtacc
     4261 atccagtcga tataaccacg cactacggtc gcttctgccg acatcggaga catcattttc
     4321 agcttctgca actctgcttc cgctttctct tttgcctctt tcggcatctt cgccgcgtcg
     4381 attttgcgct tcagggcttc gttttcgtcc ggcgcgtcgt ccatttcacc gagttctttc
     4441 tgaatagctt tcatttgctc gttcagatag tactcacgct gggatttctc catctgcttt
     4501 ttaacgcggt tgcgaatgcg tttctcaacc tgcagcagat cgatttccga ttccatcatt
     4561 gccatcagat attccagacg ttcgttaacg tcggacatct ccagaacgga ctgtttgtca
     4621 gccagtttca gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg atcgtcgatg
     4681 ctattcagcg acgtcagcac ttctggtggg atttttttgt tcagcttgat gtagccttcg
     4741 aactggctga ttgcagtacg caccagcact tcctgttccc gctcatcaat ggtcggcgac
     4801 tccagatact ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc agaaatacgc
     4861 gcgcgctgta acccctcgac cagcactttg acggtgccgt caggcagttt cagcatctgc
     4921 aatatagagg ccacggtccc gacggtgaaa agatcgttta cacccggctc atccgttgaa
     4981 gcttctttct gcgcgaccag cataattttt ttatcatggt ccatcgccgc ttccagacaa
     5041 cggatagatt tttcccgccc gacaaataag gggatgacca tgtgcggata aaccaccaca
     5101 tcgcgcagcg gcaatacggg gatttcaatg cgttcagaac gctcaggatt catagagctc
     5161 tctcttagtt taatttccgc caggtaatca gatgacacga ctgtgcttca cgccatctat
     5221 taacatgtac gtcagtatat ggggatgttt cccccacatt caacgccgag aatagaggaa
     5281 aaattaaagg ggagataaaa tccccccttt ttggttaact aattgtatgg gaatggttaa
     5341 ttattcacca gatgcctgtt gcgcttccgg cttaccataa atcagcaacg gtttgctttg
     5401 accatcaatt accgactcgt cgataaccac tttttcgacg tcttccatgg acggcagatc
     5461 gtacatggta tcgagcagtg cggcttctac gatggaacgc aggccacggg caccggtttt
     5521 acgcgccatc gctttcttag cgatagcatc cagcgcctcg tcacggaatt ccagatccac
     5581 gccttccaga ttaaacagcg cctgatactg cttggtcagg gcgtttttcg gctctttgag
     5641 gatctgaatc agagcttctt cgctcagttc attcaacgtt gctacaaccg gcagacgacc
     5701 aatgaactca gggataagac caaacttgat cagatcttcc ggttcaacct gcgccagcag
     5761 ctcgccttcg cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac caatgccgga
     5821 gccggtttct acacggtggg aaatcacttt atccagaccg gcaaacgcac cgccacaaat
     5881 aaacaggatc ttagaggtat caacctgcaa gaattcctgc tgcggatgtt tacgcccacc
     5941 ttgcggtgga acagcagcta ccgtaccttc gatcagtttc aacagtgcct gctgtacgcc
     6001 ttcaccggaa acgtctcggg taatggacgg gttgtctgac ttacgagaaa tcttgtcgat
     6061 ttcatcgatg tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc acttttgcaa
     6121 cagcttctga atgatgtttt caacgtcttc gcccacataa ccggcttcgg tcagcgtggt
     6181 tgcgtcggcc atggtgaacg ggacgtccag caggcgcgcc agcgtttcag ccagcagcgt
     6241 tttaccggaa ccggtcggac cgatcagcag aatgttactt ttgcccaact cgacaccatt
     6301 gctggtatcg ccgttgcgca gacgtttgta atggttgtat accgcgaccg ccagcacttt
     6361 tttcgcctgt tcctggccga taacgtaatc gtccaggtgg ttgcgaattt catgcggcgt
     6421 cggtagcgca ctgcgttcac gatgcggtgc aacttcttta atctcttcgc gaatgatgtc
     6481 gttacataaa tcaacacatt cgtcgcagat atacacggat ggaccggcaa tcagcttgcg
     6541 cacttcatgc tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg agccatcttt
     6601 gcgtttatct gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac acgacgacgc
     6661 aaatgccatt ctcaggcgca agccgcttac agcgttgtgc cgccctggat aagtatagcg
     6721 gcacagttgc gcctctggca tcaattacga tgggtcagaa tcgaatcgac cagaccgtat
     6781 tccaccgctt caggggcgga aaggaagcga tcgcgctcgg tatcacgttc aatctgttct
     6841 aatgattgac ccgtatgaag cgccataagt tcattcatgc gccctttaac tttcagaatt
     6901 tcacgggcat gaatttcgat atcggtcgcc tggccctggt agccgcccaa cggttggtga
     6961 atcatcacgc gcgaattcgg caggcaaaaa cgtttacctt ttgcccctgc ggtcagcaag
     7021 aaagcgccca tcgaggccgc ctggcccata cagatggtgc tgacatcagg cttgataaac
     7081 tgcatggtgt catagataga catcccggca gtgatcaccc cgcctgggga gttaatgtac
     7141 agatagatat ctttttctgg gttttccgct tctaggaaca gcatctgcgc cacaatcagg
     7201 ttcgccatgt ggtcttcaac ctggccagtc agaaaaatga cgcgttcctt aagtagacga
     7261 gaatagatat caaaagagcg ctcaccgcgt gaggtctgtt caatgaccat cggcaccagc
     7321 gccatatggg gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt ctcctggata
     7381 aaattgaaaa aacctgctgt accgattgta accttatgga cggattatca gctagtccct
     7441 gtgttatggg tacggcatca ccatatttca agcataacaa tcttttgttg ttacgctaac
     7501 accgaaagag gcttttcgca cggttccatg caaaattcat gacaaaaaaa agcccaccac
     7561 ctggcggtga cgggcctttg tgcgaattta gcgcgttatg ctgcgtaaat tacgcctgct
     7621 ggttcatcag ctcgttgaaa gtggtttctt tttcagtcac tttcgctttc gccagtacag
     7681 cttcaacagc ctgttcttcc agagcaacat tgcgcatgtt gtccatcagt tctttgtttt
     7741 tgctgtagaa ctcgataact tctttcggat cttcgtacgc agaagccatc tcttcgatca
     7801 ggcctttcac gcgctcttcg tcagctttca gctcgttggt gcggataact tcgcccagca
     7861 gcaggccaac aactacgcgg cgtttagcct gttcttcgaa cagttcgcgc ggcagttcca
     7921 gagctcgttt ttcgttgcca ccgaaacgct gtgcagcctg gcgacgcaga acgtcgattt
     7981 cgctgtcgat cagcgcagcc ggtacgtcga tgtcgttagc ttttaccaga ccttcgatcg
     8041 cctgagactt aacgcggtta cggatggcgc tcttcagctc gcgctccatg tttttacgca
     8101 cttcagcgcg cagaccttct acggaaccat cttcaacgcc gaaacgtttg atgaattctg
     8161 cagtcagttc cggcagttca cgctcttcaa ctttcttcag gttgatagcg aatttcgctg
     8221 ctttaccttt cagattttct gcgtggtatt cttccgggaa ggtcacgtcg atggtgaact
     8281 cttcgccagc tttgtggcct ttgataccgt cttcaaagcc cgggatcata cgaccctggc
     8341 ccatcgccag tacgaaatca gacgctttac cgccttcgaa ctcttcgccg tctacagaac
     8401 cggtgaagtc gatggttacg cggtcttctg cttcaacggc gccgtctttt tctttccagg
     8461 tcgcctgctg tttacgcaga gtatccagca tgccgtcaac gtcagcgtcg gtcacttcaa
     8521 cgatcggttt ttcaacttcg atcgcttcca gaccctgcag ttcaacttcc ggataaactt
     8581 caaactctac agagtaggtg aagtcttcac ccagcttgta ttcgcccgga acataagtcg
     8641 gtgcgccagc cggattgatt ttttctttaa tgatggcgtc aatgaagtta cggctcatca
     8701 ggtcacccag aacgtcctgg cgtacagacg cgccataacg ctgagcaacg atattcattg
     8761 gcactttgcc tttgcggaag ccgtcaatac gtactttttt cgcaacgttg accagctcgc
     8821 ttttaacagc ggtctcgatg ctgtcagcag cgatagtaat cgttacacgg cggccaaggc
     8881 cttgagtggt ttcaactgaa acttgcatct tgttacctca aaaaatcaca gtgctcggtc
     8941 aactcttacc ccgcaagcac aggttgttgg cttcgcggaa ccgggatgtt cttataatca
     9001 atcacattcc ctgttaccag aatcatcccg aaagcattca aataggacgc ggcattatag
     9061 cggcatcact tttatgagtc gagaacggtt attgcgcgct gtcgcgtatt ttttcacaat
     9121 tcccgggcaa ttttcgtcta aatgctgggc aaaaagcgca aaatccagac aaaacaaaac
     9181 ggcccgcagg ccgtttttca taaaatcttt agcaacatac tggaaaagcg ccgacagttg
     9241 caaatgcgtt tttacgcgat gcttcctgct ccacgacagg gaggagaggc aaagacggtg
     9301 tcctgcaacc cttcccactc cttaatagtg taagtatgca gagccagcgc atgaacggta
     9361 gtagagagtt cctccgctaa agtactgtaa atcattcgat gacgattcag aaaacgttca
     9421 cccgtaaaac gatcgctgac cagcacaact ttaaaatggc tttcagagcc ggctgggaca
     9481 ttgtgacgat agctttcatc cactacttcg aggaatacgg gttggaacgc cgcccttaat
     9541 ttttcttcta tccgctcacg tatcatcatg aaatatctcc tccgacaacg ctgagatgtc
     9601 atccatccct ttaaatacta gccgctttta ccgtttccat tgcagcttaa caacaaatat
     9661 ttaggtttcg tcctggtttt tccgtcaagc gtatgaagtt tactgattta aagattgaaa
     9721 cacaggcctc gcatcagcgt ttcttttacc aggggtgatt atttcgccaa catgatgaat
     9781 ttacatgcct tacccacttc ccctcgccgt tggcaatgtt atgatggcgg gaatttttac
     9841 caaacacgct taagagtctc tgagagcccg aacatgttca aaaaaatcct cttcccgtta
     9901 gttgctctgt ttatgcttgc aggatgcgca aaaccgccaa caactattga agtttccccg
     9961 acgattacac tgccacagca ggatccaagc ctgatgggcg tcaccgtaag cattaatggt
    10021 gccgatcagc gtaccgatca ggcgctggca aaagtcaccc gcgataatca aatcgttacc
    10081 ctgaccgcct cccgagatct gcgtttcctg ctgcaagaag tgctggaaaa acagatgacc
    10141 gcgcgtggtt acatggttgg tccgaatggc ccggttaatc tgcaaatcat cgttagccaa
    10201 ctgtatgctg acgtgtccca gggcaacgtg cgctacaaca tcgcgaccaa agcggatatc
    10261 gccatcatcg ctaccgcgca gaatggcaac aaaatgacca aaaactatcg tgccagctac
    10321 aacgttgaag gtgcgttcca ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg
    10381 ctgaccgata ccatcgctga tatgtctcag gacaccagca tccacgaatt catcaagcag
    10441 aacgcgcgtt aatttctgcc ctctggcccg gtgcaaaccg ggcctgtaga cgcccatgtc
    10501 cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc atattgctga tcctgggttt
    10561 tgcttccggg ctaccgctcg ccctgacatc cggtacctta caggcctgga tgacggtcga
    10621 gaatattgat ctcaaaacca ttggtttctt ctctctggta ggccaggctt acgtttttaa
    10681 attcctctgg tcaccgctga tggaccgcta cacgccccca ttttttgggc ggcggcgcgg
    10741 ttggctgctc gccacgcaaa tcctgttatt agtcgccatt gcggcgatgg gttttctcga
    10801 acctggcacc caactccgct ggatggcggc actggcggtg gtgatcgctt tttgctcggc
    10861 ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg
    10921 tgcgggcgcg gcaatcagcg tgctgggtta ccgtttaggg atgctggttt ccggcggctt
    10981 agccctgtgg ctggcagata aatggctggg ctggcagggc atgtactggt tgatggcggc
    11041 gctgttgatc ccttgtatta tcgcgacgtt gcttgcacca gaaccgaccg acaccattcc
    11101 tgtgccaaaa acgctggaac aagcggttgt tgcacctctg cgagatttct ttggtcgcaa
    11161 taatgcctgg cttattttgc ttcttatcgt gctgtataag ctgggcgacg cattcgccat
    11221 gagcctgaca accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt
    11281 ggttaacaaa acgcttggct tactggcgac cattgtgggc gcattgtacg gtgggatttt
    11341 gatgcagcgc ctgtcgctgt tccgggcgct gctgattttc ggcattttac aaggtgcgtc
    11401 taacgctggt tactggctgc tgtcgattac tgataagcat ctctacagca tgggcgcagc
    11461 cgtctttttc gaaaacctct gtggcgggat gggcacatca gcctttgtcg cgctgttaat
    11521 gacgctgtgt aataagtcat tttccgctac tcaatttgcc ctgctctcag cgctttccgc
    11581 tgtaggacga gtttatgtcg gccccgtggc gggttggttt gttgaagcac acggctggtc
    11641 gacattctat ctattctccg tcgccgctgc cgtaccgggg cttattttgc tgctggtttg
    11701 ccgccagacg cttgaatata cacgagtaaa tgacaacttt atctcccgta ccgaatatcc
    11761 gacaggttat gcctttgcca tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg
    11821 gttactgctg ttgacgatgg acgcgctgga tttgacgcac ttctctttcc tgcctgctct
    11881 gctggaagtc ggggttttag tcgccctttc tggcgtcgtg cttggtggtt tgctggatta
    11941 tctggctcta cgaaaaacgc atctgacgta atctgtaaat attatttagc tgtagtaatc
    12001 actcgccgta attattacgg ctaaataaac atcagtatcg cttaataata tttgttcttt
    12061 tgtgcggctt agcgtttgga aattattgac gcaatttatg aattctattt tttcttacac
    12121 gattcagcta atgagtcttt attttccccc ccacccaatt gtcaccataa tgataattat
    12181 ttgttaaaca attgttttat tccagattgg ttataccaat tgcccgtcca aactccttct
    12241 gcactcccct ttcgttataa cgcccttttg caacagcttc ttaaaatcaa cttgatatgt
    12301 tttgcaacat atgtaacctg acagccaaat ccaagtaaca ggaatttaat catgtttaca
    12361 gtaatgtaac ctccccgtaa aatgcccaca cactttaaac gccaccagat cccgtggaat
    12421 tgaggtcgtt aaatgagact caggaaatac aataaaagtt tgggatggtt gtcattattt
    12481 gcaggcactg tattgctcag tggctgtaat tctgcgctgt tagatcccaa aggacagatt
    12541 ggtctggagc aacgttcact gatactgacg gcatttggcc tgatgttgat tgtcgttatt
    12601 cccgcaatct tgatggctgt tggtttcgcc tggaagtatc gtgcgagcaa taaagatgct
    12661 aagtacagcc cgaactggtc acactccaat aaagtggaag ctgtggtctg gacggtacct
    12721 atcttaatca tcatcttcct tgcggtactg acctggaaaa ccactcacgc tcttgagcct
    12781 agcaagccgc tggcacacga cgagaagccc attaccatcg aagtggtttc catggactgg
    12841 aaatggttct tcatctaccc ggaacagggc attgcaaccg tgaatgaaat cgctttcccg
    12901 gcgaacactc cggtgtactt caaagtgacc tccaactccg tgatgaactc cttcttcatt
    12961 ccgcgtctgg gtagccagat ttatgccatg gccggtatgc agactcgcct gcatctgatc
    13021 gccaacgaac ccggtactta tgacggtatc tccgccagct acagcgggcc gggcttctca
    13081 ggcatgaagt tcaaagctat tgcaacaccg gatcgcgccg aattcgacca atgggtcgca
    13141 aaagcgaaac agtcgccgaa caccatgtct gacatggctg cgttcgaaaa actggccgcg
    13201 cctagcgaat acaaccaggt ggaatatttc tccaacgtga aaccagactt gtttgccgat
    13261 gtgattaaca agtttatggc tcacggtaag agcatggaca tgacccagcc agaaggtgag
    13321 cacagtgcac acgaaggtat ggaaggcatg gacatgagcc acgcggaatc cgcccattaa
    13381 aggggttgag gaagaataaa gatgttcgga aaattatcac ttgatgcagt cccgttccat
    13441 gaacctatcg tcatggttac gatcgctggc attattttgg gaggtctggc gctcgttggc
    13501 ctgatcactt acttcggtaa gtggacctac ctgtggaaag agtggctgac ctccgtcgac
    13561 cataaacgcc tcggtatcat gtatatcatc gtggcgattg tgatgttgct gcgtggtttt
    13621 gctgacgcca ttatgatgcg tagccagcag gctcttgcct cggcgggcga agcgggcttc
    13681 ctgccacctc accactacga tcagatcttc accgcgcacg gcgtgattat gatcttcttc
    13741 gtggcgatgc ctttcgttat cggtctgatg aacctggtgg ttccgctgca gatcggcgcg
    13801 cgtgacgttg cgttcccgtt cctcaacaac ttaagcttct ggtttactgt tgttggtgtg
    13861 attctggtta acgtttctct cggcgtgggc gaatttgcgc agaccggctg gctggcctat
    13921 ccaccgctat cgggaataga gtacagtccg ggagtcggtg tcgattactg gatatggagt
    13981 ctccagctat ccggtatagg tacgacgctt accggtatca acttcttcgt taccattctg
    14041 aagatgcgcg caccgggcat gaccatgttc aagatgccag tatttacctg ggcatcactg
    14101 tgcgcgaacg tactgattat tgcttccttc ccaattctga cggttaccgt cgcgttgttg
    14161 accctggatc gctatctggg cacccatttc tttaccaacg atatgggtgg caacatgatg
    14221 atgtacatca acctgatttg ggcctggggc cacccggaag tttacatcct gatcctgcct
    14281 gttttcggtg tgttctccga aattgcggca accttctcgc gtaaacgtct gtttggttat
    14341 acctcgctgg tatgggcaac cgtctgtatc accgtgctgt cgttcatcgt ttggctgcac
    14401 cacttcttta cgatgggtgc gggcgcgaac gtaaacgcct tctttggtat caccacaatg
    14461 attatcgcca tcccgaccgg ggtgaagatc ttcaactggc tgttcaccat gtatcagggc
    14521 cgcatcgtgt tccattctgc gatgctgtgg accatcggtt ttatcgtcac tttctcggtg
    14581 ggcgggatga ctggcgtgct gctggcagta ccgggcgcgg acttcgttct gcataacagc
    14641 ctgttcctga ttgcgcactt ccataacgtt atcatcggcg gcgtggtctt cggctgcttc
    14701 gcagggatga cctactggtg gcctaaagcg ttcggtttca aactgaatga aacctggggt
    14761 aaacgcgcgt tctggttctg gatcatcggc ttcttcgttg cctttatgcc gctgtatgcg
    14821 ctgggcttca tgggcatgac ccgtcgtttg agccagcaga ttgacccgca gttccacacc
    14881 atgctgatga ttgcagctag cggtgcagta ctgattgcgc tgggtattct ctgcctcgtt
    14941 attcagatgt acgtttctat tcgcgaccgc gaccagaacc gtgacctgac tggcgacccg
    15001 tggggtggcc gtacgctgga gtgggcaacc tcttccccgc ctccgttcta taactttgcc
    15061 gttgtgccgc acgttcacga gcgtgatgca ttctgggaaa tgaaagagaa aggcgaagcg
    15121 tataaaaagc ctgaccacta tgaagaaatt catatgccga aaaacagcgg tgcaggtatc
    15181 gtcattgcag ctttctccac catcttcggt ttcgccatga tctggcatat ctggtggctg
    15241 gcgattgttg gcttcgcagg catgatcatc acctggatcg tgaaaagctt cgacgaggac
    15301 gtggattact acgtgccggt ggcagaaatc gaaaaactgg aaaaccagca tttcgatgag
    15361 attactaagg cagggctgaa aaatggcaac tgatactttg acgcacgcga ctgcccacgc
    15421 gcacgaacac gggcaccacg atgcaggcgg aaccaaaatc ttcggatttt ggatctacct
    15481 gatgagcgac tgcattctgt tctcaatctt gtttgctacc tatgccgttc tggtgaacgg
    15541 caccgcaggc ggcccgacag gtaaggacat tttcgaactg ccgttcgttc tggttgaaac
    15601 tttcttgctg ttgttcagct ccatcaccta cggcatggcg gctatcgcca tgtacaaaaa
    15661 caacaaaagc caggttatct cctggctggc gttgacctgg ttgtttggtg ccggatttat
    15721 cgggatggaa atctacgaat tccatcacct gattgttaac ggcatgggtc cggatcgcag
    15781 cggcttcctg tcagcgttct ttgcgctggt cggcacgcac ggtctgcacg tcacttccgg
    15841 tcttatctgg atggcggtgc tgatggtaca aatcgctcgt cgcggcctga ccagcactaa
    15901 ccgtacccgc atcatgtgcc tgagcctgtt ctggcacttc ctggatgtgg tttggatctg
    15961 tgtgttcact gttgtttatc tgatgggggc gatgtaatga gtcattctac cgatcacagc
    16021 ggcgcgtccc atggcagcgt aaaaacctac atgacaggct ttatcctgtc gatcattctg
    16081 acggtgattc cgttctggat ggtgatgaca ggagctgcct ctccggccgt aattctggga
    16141 acaatcctgg caatggcagt ggtacaggtt ctggtgcatc tggtgtgctt cctgcacatg
    16201 aataccaaat cagatgaagg ctggaacatg acggcgtttg tcttcaccgt gctaatcatc
    16261 gctatcctgg ttgtaggctc catctggatt atgtggaacc tcaactacaa catgatgatg
    16321 cactaagagc ggcggttatg atgtttaagc aatacctgca agtaacgaaa ccaggcatca
    16381 tctttggcaa cctgatctcg gtgattgggg gattcctgct ggcctcaaag ggcagcattg
    16441 attatcccct gtttatctac acgctggttg gggtgtcact ggttgtggcg tcgggttgtg
    16501 tgtttaacaa ctacatcgac agggatatcg acagaaagat ggaaaggacg aagaatcggg
    16561 tgctggttaa aggcctgatc tctcctgctg tctcgctggt gtacgccacc ttgctaggct
    16621 ttgctggctt tatgctgctg tggtttggcg cgaatccgct ggcctgctgg ctgggggtga
    16681 tgggctttgt ggtttatgtc ggcgtttata gcctgtacat gaaacgccac tcggtctacg
    16741 gcacattgat tggttcgctc tccggcgctg cgccgccggt gattggctac tgtgcagtaa
    16801 ccggcgagtt cgatagcggc gcagcgatcc tgctggctat cttcagcctg tggcagatgc
    16861 ctcactccta tgccatcgcc attttccgct ttaaggatta tcaggcggca aacattccgg
    16921 tattgccagt ggtaaaaggc atttcggtgg cgaagaatca catcacgctg tatatcatcg
    16981 cctttgccgt tgccacgctg atgctctctc ttggcggtta cgctgggtat aaatatctgg
    17041 tggtcgccgc ggcggtgagc gtctggtggt taggtatggc cctgcgcggt tataaagttg
    17101 ctgatgacag gatctgggcg cgcaagctgt tcggcttctc tatcatcgcc atcacagccc
    17161 tctcggtgat gatgtccgtt gattttatgg tgccggactc gcatacgctg ctggctgctg
    17221 tttggtaaca caacctctct attaaaaagg tgctacggca ccttttttat tgtctgagat
    17281 cagagatacg gctaataacc gtatgtcttg acagagtgag cagatgaaca taaattaatc
    17341 atacggttga tagccgtatt atgtatgaga tcgactccct acgatttcat tcagttaatc
    17401 aaagaggtca taatgcctgg aaaaacagcc actctacccg atgtcgataa aacgctgaaa
    17461 aatgcgcggg tcgaactcaa aactagtccc gacgccaaaa acaaacttcg tgaagctgcg
    17521 caagccgttg gggtagattt aagcgctttt attttgagcg ctgctatgga acgcgccgaa
    17581 agtgtgcttg ataaccaacg tcgccgtgag ctttcgaatc aaagctggga actgatgaac
    17641 caactcatcg ctgaacctgc tcaaccgacg ctcgccctca aggcgttaat gaaaaggaaa
    17701 aacagcgatg gtcgacaagc atgaagagat tactctgccc atagtcctct cctgtaatta
    17761 tcagtctgat attacttatc ctgggcaaaa acagtttgat tgcggtaact ctgttatcga
    17821 taaatttgta cgcgcatcgc taaagaaaag tgtgcgtaat agcgactgtg cggctaaagc
    17881 acttattgac agacaaagtg gtgaactgat cggcatctgt acttttacgg catattcgct
    17941 ggaaaaacaa cgcgtttctg gcgtccttca gggttcacaa ccttcagaaa ttggtgttgt
    18001 cagattagtc atgttggggg tagcacggaa gtatcaaaag cggggctttg gtcaggacct
    18061 actatgtgat ttttttgaac atgtaaaaat aattcaccag gcattaccaa ttaaaggggt
    18121 ttatcttgat gctgaccctg ccgccattaa tttttatgct cgtctcggct ttgttcagct
    18181 ttcagcgaca ccaaatgctt ttggtgctgt acctatgttt ttggcgattc agcatattct
    18241 cgcggcttag attattatcg ccacccgcca gggcaagttt tgccttagcc gggggctgag
    18301 cagcgttacc acttttgttc aattgccccc gctttcagca actccacatc cttgccgtta
    18361 atggttgccg ttgcggcaac gattgtggtc cttacagttg cacaagattt tcgctcaaat
    18421 tccggtttat aaagaataag agacgaatta ctggtaaatg aaccaaatag gtaatcagta
    18481 aagctaatat tcagccggtt taaaccgaaa ggcgcgaagc caaccgtttg gttaatctcc
    18541 ttaccggatg ggcgacgaat gctgaatgtc acgcgaagat tggccacttc gcctaattta
    18601 ttggtggtgg cgaagaggct atccatgcag gaaaaacctt tatctaaaaa attatctcgc
    18661 atcccagaac gggtaaccgt aatcacgggt aacgtatcgt caaccgtttt tctgtcaatc
    18721 atgtcttttc gacaagtagc cagaaagtct tccgggccac cataaatgtg gacaccttta
    18781 tcaagatatt ccttcgcttt cttacaatct acctcaacat actgaccatt ggcgtacata
    18841 atgccc
//
LOCUS       DAZRVY010000075        18213 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZRVY010000075 DAZRVY010000000
VERSION     DAZRVY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.075"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHXOX_002682"
     CDS             15..1091
                     /locus_tag="ACHXOX_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7006106.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHXOX_002683"
     CDS             complement(1484..1825)
                     /locus_tag="ACHXOX_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR7006107.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXOX_002684"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXOX_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR7006108.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXOX_002685"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXOX_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR7006109.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHXOX_002686"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHXOX_002686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR7006110.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXOX_002687"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXOX_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR7006111.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXOX_002688"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXOX_002688"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR7006112.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXOX_002689"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXOX_002689"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR7006113.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHXOX_002690"
     CDS             complement(7167..7826)
                     /locus_tag="ACHXOX_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR7006114.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXOX_002691"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXOX_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR7006115.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXOX_002692"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXOX_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR7006116.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHXOX_002693"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHXOX_002693"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR7006117.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXOX_002694"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXOX_002694"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR7006118.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXOX_002695"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXOX_002695"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR7006119.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXOX_002696"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXOX_002696"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR7006120.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXOX_002697"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXOX_002697"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR7006121.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXOX_002698"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXOX_002698"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR7006122.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHXOX_002699"
     CDS             complement(17270..18112)
                     /locus_tag="ACHXOX_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR7006123.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4669 a         4574 c         4394 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtgggtc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaa
//
LOCUS       DAZRVY010000076        17932 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZRVY010000076 DAZRVY010000000
VERSION     DAZRVY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.076"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            217..895
                     /locus_tag="ACHXOX_002700"
                     /pseudo
     CDS             217..895
                     /locus_tag="ACHXOX_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            939..5789
                     /locus_tag="ACHXOX_002701"
     CDS             939..5789
                     /locus_tag="ACHXOX_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR7006124.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6157..7812
                     /gene="lldP"
                     /locus_tag="ACHXOX_002702"
     CDS             6157..7812
                     /gene="lldP"
                     /locus_tag="ACHXOX_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR7006125.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7812..8588
                     /gene="lldR"
                     /locus_tag="ACHXOX_002703"
     CDS             7812..8588
                     /gene="lldR"
                     /locus_tag="ACHXOX_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR7006126.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8585..9775
                     /gene="lldD"
                     /locus_tag="ACHXOX_002704"
     CDS             8585..9775
                     /gene="lldD"
                     /locus_tag="ACHXOX_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR7006127.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9959..10432
                     /gene="trmL"
                     /locus_tag="ACHXOX_002705"
     CDS             9959..10432
                     /gene="trmL"
                     /locus_tag="ACHXOX_002705"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR7006128.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10485..11306)
                     /gene="cysE"
                     /locus_tag="ACHXOX_002706"
     CDS             complement(10485..11306)
                     /gene="cysE"
                     /locus_tag="ACHXOX_002706"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR7006129.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11386..12405)
                     /gene="gpsA"
                     /locus_tag="ACHXOX_002707"
     CDS             complement(11386..12405)
                     /gene="gpsA"
                     /locus_tag="ACHXOX_002707"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR7006130.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12405..12872)
                     /gene="secB"
                     /locus_tag="ACHXOX_002708"
     CDS             complement(12405..12872)
                     /gene="secB"
                     /locus_tag="ACHXOX_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR7006131.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12935..13186)
                     /gene="grxC"
                     /locus_tag="ACHXOX_002709"
     CDS             complement(12935..13186)
                     /gene="grxC"
                     /locus_tag="ACHXOX_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR7006132.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13328..13759)
                     /locus_tag="ACHXOX_002710"
     CDS             complement(13328..13759)
                     /locus_tag="ACHXOX_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR7006133.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14004..15548
                     /gene="gpmM"
                     /locus_tag="ACHXOX_002711"
                     /gene_synonym="pgmI"
     CDS             14004..15548
                     /gene="gpmM"
                     /locus_tag="ACHXOX_002711"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR7006134.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15582..16841
                     /gene="envC"
                     /locus_tag="ACHXOX_002712"
     CDS             15582..16841
                     /gene="envC"
                     /locus_tag="ACHXOX_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR7006135.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16845..17804
                     /locus_tag="ACHXOX_002713"
     CDS             16845..17804
                     /locus_tag="ACHXOX_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR7006136.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17791..>17932)
                     /locus_tag="ACHXOX_002714"
     CDS             complement(17791..>17932)
                     /locus_tag="ACHXOX_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR7006137.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4512 a         4633 c         4678 g         4109 t
ORIGIN      
        1 ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac acagacatct
       61 tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca
      121 tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca actctaccgg
      181 cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc
      241 agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc
      301 aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc tacttgaaaa
      361 acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa ctggcataat
      421 aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac aaaacacttt
      481 tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca atggttcggt
      541 ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg ttattcatat
      601 ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg cgacatgggg
      661 tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac ttttcaacac
      721 atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta atttgaccct
      781 gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca tggaaaatat
      841 tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa aataaatgca
      901 gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata tttaaagtta
      961 tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa agccgtggca
     1021 agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga atgttgtcgt
     1081 cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat tacggtagtg
     1141 gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat acttttatga
     1201 acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc caatatagct
     1261 ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt ggggttagtg
     1321 caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca ttgggccaat
     1381 actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt gctatggggg
     1441 actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact aaagctactg
     1501 agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat tcaatggcgc
     1561 tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt agcagtgtag
     1621 ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca gcgaacgcta
     1681 ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc aataatgcga
     1741 aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat aaagtcaata
     1801 ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc ataggccagg
     1861 gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag tcgagaggct
     1921 taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt cttgcgcttg
     1981 gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat tccattgcgg
     2041 atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc gttaatgtga
     2101 aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag ctttatgcca
     2161 ttagcgactc ggtagcaaaa acgcttggag gaggggctgc agtagatgtt gatgacggta
     2221 ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta ggggctgcgc
     2281 tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa tacagcgctg
     2341 ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat ggcacgattt
     2401 cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat gacgatgtcg
     2461 aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat acagcaagta
     2521 ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac cttcaggctg
     2581 atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc caggatacca
     2641 ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact gatgctgtta
     2701 acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc aatatcgcca
     2761 ataacacttc caatattgcc actaacacca ccaacatctc taatctgact gagacggtga
     2821 ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc acggcagctc
     2881 atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg acgactggca
     2941 gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg gcgacgaata
     3001 ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg gtgactaatc
     3061 ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca gctcatggca
     3121 acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca accagttccg
     3181 atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc tacttcggcg
     3241 gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa atcggtgaaa
     3301 caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt agcacgtctc
     3361 tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa cacggtacta
     3421 atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac tccagttctg
     3481 acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat gcgctggggg
     3541 gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc attgctaatg
     3601 ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt gacgacgctc
     3661 tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac ggaaaagata
     3721 aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc agcagcgacg
     3781 cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg ctgggtggtg
     3841 acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt gcgaacgccg
     3901 agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg ctgtgggatg
     3961 agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa gccagcatca
     4021 tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg aacggttctc
     4081 agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat cagctcgctg
     4141 gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac gttcgtacta
     4201 acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct acagctgtag
     4261 gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac agcagcagca
     4321 ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg attgccaaag
     4381 gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac accactgacg
     4441 gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa atcatcaacg
     4501 tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag aacgctattg
     4561 gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca gaagactcac
     4621 tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat ggtaatgcag
     4681 gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc attgctatcg
     4741 gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt tctcagacca
     4801 cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag aactctgtag
     4861 gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc gcagctggtt
     4921 cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag cgcgtagcgc
     4981 aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat actcgcgtta
     5041 ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag tacttcaaga
     5101 ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct attggttctg
     5161 gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt gcagaggaag
     5221 aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac gtagctgccg
     5281 gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa gcaggcggcg
     5341 tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc ctcggtggtg
     5401 gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac aacgacgcgg
     5461 tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc gatcagcgga
     5521 tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt ggtatcgctt
     5581 ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc atggcttcta
     5641 ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg atggtgagcg
     5701 ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt gaatactccg
     5761 ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga ctaagggcaa
     5821 acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc gggtcaacaa
     5881 tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct ccccccacaa
     5941 gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc ccaattaaaa
     6001 ctcacatcaa tattgccaat acataacatt tagttaacta ttcattgtca ttatccctac
     6061 acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga gcaacagact
     6121 cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca acaaaactac
     6181 gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat cctgtttttc
     6241 ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg gacggtagca
     6301 atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc gctggcctcg
     6361 gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat tgcagcagtg
     6421 ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc gtctattctt
     6481 tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt cggcgctttc
     6541 cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt gctggtcggc
     6601 ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac cgcgccagtg
     6661 gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg catcgacagc
     6721 tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat cgtgctgttc
     6781 tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc tgcggtcgtg
     6841 gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat tgggccggag
     6901 ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt cctcaaacgc
     6961 tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcaaagat tgatatgacg
     7021 ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc gttcctgttc
     7081 ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt cgcatcgggt
     7141 ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa actggttgcc
     7201 cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa gtttgactgg
     7261 ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt ctggctgaag
     7321 atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact ggctctaccc
     7381 atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc cggactgtca
     7441 tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt ctcgccgttc
     7501 ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc cctgttcgcc
     7561 gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct ggttgccgcc
     7621 aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc tatcgcctgt
     7681 gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt caaacacagc
     7741 ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt cttaacgtgg
     7801 atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc gatcgtgtgc
     7861 gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct gagcgccaac
     7921 tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca aaactggtga
     7981 gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg cgtcatgaca
     8041 catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat gatccggatt
     8101 acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca tggcatgcgg
     8161 caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa gcaacgctaa
     8221 gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg attgccgaag
     8281 cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc ctgcaatcct
     8341 cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca caactgaccg
     8401 aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg gcgcgtaaag
     8461 caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat gaagatcagg
     8521 ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg agggagaaaa
     8581 acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca ttctgccgcc
     8641 gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc gccgcaacgt
     8701 ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt ccgacttaag
     8761 cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg caccggtggg
     8821 tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg cggacgcgca
     8881 tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag tcgcgccagc
     8941 catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct ttatgcgtaa
     9001 cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg tggatatgcc
     9061 gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa atgcggcaat
     9121 gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg gcctgaacgg
     9181 tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg gactggaaga
     9241 ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag accttgagtg
     9301 gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc cggaagatgc
     9361 gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg gtggccgcca
     9421 gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg cggtgaaagg
     9481 tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg tgcgtatgat
     9541 tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc tggcaacagc
     9601 gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga aagtggcgat
     9661 gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg tgcaggggct
     9721 gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg catagtcgtt
     9781 tgcgccccct caccctcagg gagaggggac cgttcggcgc tgtatgtact ccctcactct
     9841 gaaacgacac cgcactcttt ttttctccct cgcccctccg gggagggccg gggtgagggg
     9901 aaaaggccgc acggttcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat
     9961 gctaaacatc gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct
    10021 ttgcgctaat accggctttc gtctgcatat catcgaaccg atgggatttg cctgggacga
    10081 taagcgcctg cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca
    10141 tgactatcgc gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa
    10201 aggtacgcct gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc
    10261 ggaaacacgc ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg
    10321 cattccgatg gtgccggaca gccgcagcat gaatctgtcc aatgcggtgt cggtagtggt
    10381 gtatgaagcc tggcggcagc tgggatatcc gggagcgttg ttgagagatt agttactgta
    10441 tgccggatag atgattacat cgcatccggc acgatcacag gacattagat cccatcccca
    10501 tactcaaatg tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg
    10561 ctgtctggtt taccgacaat acgagccgga acgccagcgg cggttgtatg cggcggcacc
    10621 ggttgcagca ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg
    10681 aggattttcg cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca
    10741 ccagatttac ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt
    10801 accgccgttt cacccacaac gatgcccgtg gcgtggtcga gcataatgcc acggccaatt
    10861 tttgctgccg ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt
    10921 gccaatgcgc gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca
    10981 tgaaaaccct tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta
    11041 cgcaccgcct gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct
    11101 tcttccacca cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc
    11161 agcatgtagc tcagtgcact accaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa
    11221 ctggccagca ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag
    11281 acaatttcca gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc
    11341 gttctgcgcg ggccgggtca tagcggtaac aaaggttccc tggggttagt ggctgctgcg
    11401 ctcgtccttg cgtgcacgac ctaataacgt caatgctgcc tcgcgcgcgt tttttccgca
    11461 atataatact tgataaattt cctcggttat tggcatttct acgccgaagc gatgcgccag
    11521 ttcgcggact tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc
    11581 gctttgtaca tccatgccct gaccgagcat catgccaaaa cggcggttac gcgactggtt
    11641 gtcggtacag gtaagcacca gatcgcccag ccccgccatg cccataaagg tggcaggatc
    11701 ggcacccagc gccgcgccaa gacgcgacat ttcagccagc ccacgggtga tcagtgccgt
    11761 acgcgcattc gcaccaaaac cgataccgtc ggacatcccc gcgccaatgg caatgacgtt
    11821 tttcaccgca ccgccaagct gcacaccgat gaaatccggg ttgctgtaaa cgcggaaact
    11881 tttgccacag tgcagcaatt gttggagatc atcggcaaag gtctggtcgg tagaggccaa
    11941 cgaaatagcc gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac
    12001 cgccagcgga atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt
    12061 ttccgcttcc agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt
    12121 aatctggcgc agcacttcac caaagacatg gctgggtacg acgacgagaa tattacggct
    12181 ggctgccagc gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc
    12241 ggggagaaac gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg
    12301 gccccagagg acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta
    12361 cgagccggca ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt
    12421 tcttcagtac cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta
    12481 atcggcgcaa ggttcagttg cgggaatgta ccgcgggata ccacgctggt gatgcactca
    12541 cgagcatacg ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg
    12601 ccttcgatac ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt
    12661 tcttcgccca gggaggccgt tacggtaaca cgcagtacca cttcgtatac gtcatctgcc
    12721 agttgggaag atgccgtatc cagatcaagt ttaacttctg gttgccaatc tttctggaaa
    12781 acgtgcggcg cgttcggcgc ttcgaaagag atatccttgg tataaatacg ttggatctgg
    12841 aaagtcattt cagtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt
    12901 aaatactgcg caatgtaatg ccaacgttca cacgttattt cagcagggga tccagtccac
    12961 cacgtgcatc caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa
    13021 tctggggaac cgtggtgcga ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc
    13081 catcgatcgg caactcctga aaactcacgc ccttgctgct cagcagtgct tttgcacgat
    13141 ggcaatacgg gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt
    13201 ttttttaccc tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct
    13261 tataacaata agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac
    13321 aggtaaatta tttgccgcgc accagaggca agttttcgcc agcccagcca gcaacgccct
    13381 ctttcagcac gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca ggctcctggc
    13441 actgcatgcc agaaccgtct accacgataa ccggtttgtc tttgtgcttc tcaagctcac
    13501 caacattgtt ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt
    13561 tacggaagtc atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac
    13621 gcgtagcttc accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca
    13681 caagaaccgc caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa
    13741 attgcataat ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg
    13801 aaaggagtat acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt
    13861 tgcggggcac gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat
    13921 attttgatct tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga
    13981 ctcttgagta tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc
    14041 tggatggcta tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc
    14101 cggtaatgga tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg
    14161 aagtcggtct gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg
    14221 ccggccgcat cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt
    14281 tctttgctaa tccagtgctg actggcgcgg tagataaagc gaaaaacgca ggtaaagcag
    14341 tacacattat gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg
    14401 cgatggtaga actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg
    14461 acggtcgcga caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat
    14521 ttgctgcgct gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc
    14581 gcgataaccg ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg
    14641 agtttcaggc cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg
    14701 acgagttcgt gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag
    14761 acggcgatgc gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa atcactcgtg
    14821 cattcgtgaa cgctgatttc gatggcttcg cgcgtaagaa agtggttaac gtcgatttcg
    14881 tgatgctgac cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc
    14941 tggttaacac cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg
    15001 aaaccgaaaa atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca
    15061 aaggcgaaga tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg
    15121 aaatgagctc cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg
    15181 acaccattat ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag
    15241 cggcggttaa agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg
    15301 agtccgtggg tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg
    15361 atccggcaac gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttacg
    15421 ttggtgataa gaacgtgaaa gcggttgcag gcggcaaact ttctgacatc gcgccgacca
    15481 tgttgtcgct gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg
    15541 tggaataatc cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac
    15601 cgcgcaggtt tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt
    15661 tgtgcgcctt ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata
    15721 tcgccacaaa agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac
    15781 aattgaaaaa gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa
    15841 acacgctcaa tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg
    15901 agcagcaaaa agccgcccag gagcgcagcc tcgccgcaca actggatgcc gcattccgtc
    15961 agggcgagca taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc
    16021 gtttacaggc ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga
    16081 agcaaacgcg tgaagaagtc gccatgcagc gtgctgaact ggaagagaaa caaagcgagc
    16141 aacaaacgct gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg
    16201 agcgtaaaaa gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa
    16261 gtgaactgcg tgccaacgaa tcccgtctgc gtaacagtat tgcccgtgcg gaagccgcgg
    16321 cgaaagcgcg tgctgaacga gaagcgcgtg aggctcaggc agttcgcgac aggcagaaag
    16381 aagcgacgcg caaaggcacc acctacaaac cgaccgaaag cgaaaaatcg ctgatgtccc
    16441 gaactggtgg cctgggtgcg ccgcgtggtc aggcattctg gccagttcgt ggccctacgc
    16501 tgcatcgcta tggcgagcag ctacagggtg aattacgctg gaaaggaatg gttatcggtg
    16561 cctctgaagg tactgaagtt aaagcaattg ccgacggtcg ggtgattctg gctgactggc
    16621 tgcaaggcta cggtctggtg gtggtggttg agcacggtaa aggcgatatg agtctttacg
    16681 gctataatca aagcgcgctg gtgagcgttg gttcgcaggt tcgtgcgggc cagccaattg
    16741 cactggtggg cagcagtggc ggtcagggca gaccttcact ctatttcgaa attcgccgcc
    16801 agggtcaggc cgtcaatcca cagccgtggt tgggaagata agttttgttt ccatttcgtc
    16861 gtaacgttct tgcatttgcc gctctgttgg cgctctcctc ccctgtaatt gctggcaaac
    16921 ttgccatcgt cattgatgat tttgggtatc gcccgaacaa cgaaaaccag gtgctggcga
    16981 tgccttccgc tatctccgtc gctgtattac ccgattcacc gcacgccaga gagatggcga
    17041 ccaaagcgca taacagcggt cacgaagtgt tgattcatct cccgatggca ccgttgagta
    17101 aacagccgct ggagaaaaat acgctgcgcc cggagatgag cagcgacgaa attgagcgca
    17161 ttattcgtag tgcggtcaat aacgtgccct atgccgtggg gatcaataac cacatgggca
    17221 gcaagatgac ctctaacctg tttggtatgc agaaagtgat gcaggcgctg gagcgttata
    17281 atctttactt cctcgacagc gtaaccattg gtaataccca ggcgatgcgc gccgcgcaag
    17341 gcactggcgt gaaggtgatc aaacggaagg tgttcctcga cgattcacag aacgaagcgg
    17401 acatccgtgt gcaattcaat cgcgcaattg acctagcgcg tcgcaacgga tcaaccattg
    17461 ccattgggca tcctcaccct tcaacggtac gcgtgttgca acagatggtt tataacctgc
    17521 cgccagacat tacgctggtg aaagccagca gcttgctgaa tgaaccgcag gttgatacgt
    17581 ctacaccgcc gaaaaacact gtgcccgaca caccgcgtaa tccgttccgt ggcgtgaagc
    17641 tgtgcaaacc gaagaaaccg atagaacctg tttatgctaa tcgcttcttt gaagtattaa
    17701 gcgaaagcat cagccagagc acgctgatcg tttacttcca gcatcagtgg caaggctggg
    17761 gcaaacagcc tgaagcggcg aagcttaacg ctagcgtaaa ttaaaggcgc tacgcgtaat
    17821 gcgatggtgc gaccgcgttt tgtcgcgcca ttgccataag cggaaagacc acagtaacgc
    17881 ctgataaccc acctttacgc tgcgcacatt ggtaatcagg cgcttataca ta
//
LOCUS       DAZRVY010000077        17457 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZRVY010000077 DAZRVY010000000
VERSION     DAZRVY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.077"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..232
                     /locus_tag="ACHXOX_002715"
     CDS             <1..232
                     /locus_tag="ACHXOX_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR7006138.1"
                     /translation="VFLYTIFRDNTKDTNDLLTRVATLETTIAVLESSISRIEDDQDK
                     MRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(271..720)
                     /locus_tag="ACHXOX_002716"
     CDS             complement(271..720)
                     /locus_tag="ACHXOX_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR7006139.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIGYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            757..1191
                     /locus_tag="ACHXOX_002717"
     CDS             757..1191
                     /locus_tag="ACHXOX_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006140.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            1201..1701
                     /locus_tag="ACHXOX_002718"
     CDS             1201..1701
                     /locus_tag="ACHXOX_002718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006141.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            1777..2088
                     /locus_tag="ACHXOX_002719"
     CDS             1777..2088
                     /locus_tag="ACHXOX_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006142.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            2085..2378
                     /locus_tag="ACHXOX_002720"
     CDS             2085..2378
                     /locus_tag="ACHXOX_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006143.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            2439..3233
                     /locus_tag="ACHXOX_002721"
     CDS             2439..3233
                     /locus_tag="ACHXOX_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7006144.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYPEVEIYSPIL"
     gene            complement(3259..3714)
                     /locus_tag="ACHXOX_002722"
     CDS             complement(3259..3714)
                     /locus_tag="ACHXOX_002722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006145.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQLSPLEGLTICIVSLI
                     LFVSVLVSALLNYKKIKKPDVREIIKQSHSVFRENEETITITTTIKKAP"
     gene            3749..4474
                     /locus_tag="ACHXOX_002723"
     CDS             3749..4474
                     /locus_tag="ACHXOX_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR7006146.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            4967..5713
                     /gene="mipA"
                     /locus_tag="ACHXOX_002724"
     CDS             4967..5713
                     /gene="mipA"
                     /locus_tag="ACHXOX_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR7006147.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            5803..6657
                     /gene="yeaE"
                     /locus_tag="ACHXOX_002725"
     CDS             5803..6657
                     /gene="yeaE"
                     /locus_tag="ACHXOX_002725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR7006148.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(6708..7592)
                     /locus_tag="ACHXOX_002726"
     CDS             complement(6708..7592)
                     /locus_tag="ACHXOX_002726"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR7006149.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(7676..8671)
                     /gene="gapA"
                     /locus_tag="ACHXOX_002727"
     CDS             complement(7676..8671)
                     /gene="gapA"
                     /locus_tag="ACHXOX_002727"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR7006150.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9013..9426
                     /gene="msrB"
                     /locus_tag="ACHXOX_002728"
     CDS             9013..9426
                     /gene="msrB"
                     /locus_tag="ACHXOX_002728"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR7006151.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9468..9740
                     /locus_tag="ACHXOX_002729"
     CDS             9468..9740
                     /locus_tag="ACHXOX_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR7006152.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9901..10542)
                     /gene="pncA"
                     /locus_tag="ACHXOX_002730"
     CDS             complement(9901..10542)
                     /gene="pncA"
                     /locus_tag="ACHXOX_002730"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR7006153.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(10553..11569)
                     /gene="ansA"
                     /locus_tag="ACHXOX_002731"
     CDS             complement(10553..11569)
                     /gene="ansA"
                     /locus_tag="ACHXOX_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR7006154.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(11736..13592)
                     /gene="sppA"
                     /locus_tag="ACHXOX_002732"
     CDS             complement(11736..13592)
                     /gene="sppA"
                     /locus_tag="ACHXOX_002732"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR7006155.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            13753..14304
                     /locus_tag="ACHXOX_002733"
     CDS             13753..14304
                     /locus_tag="ACHXOX_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR7006156.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            14421..15464
                     /gene="selD"
                     /locus_tag="ACHXOX_002734"
     CDS             14421..15464
                     /gene="selD"
                     /locus_tag="ACHXOX_002734"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR7006157.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            15469..17430
                     /gene="topB"
                     /locus_tag="ACHXOX_002735"
     CDS             15469..17430
                     /gene="topB"
                     /locus_tag="ACHXOX_002735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR7006158.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         4751 a         3908 c         4310 g         4488 t
ORIGIN      
        1 cgtattcctt tatactatct ttagagataa tacaaaggat acgaatgatc tattaactcg
       61 cgttgctact cttgaaacta ccattgctgt actggaaagt agtatttccc gtattgagga
      121 tgatcaggat aagatgagag atacactaag taaactggaa acacaaatcc atgaacttga
      181 tgttaagatt gaaaggatta ttgcaattct tcagcagaaa caacgaccat aaaaaaaggc
      241 atagtatgtc attcgtacta tgccttttct ttatttgttt ttaagtactg caatcatgga
      301 attcacacgg tttggtgtct gcctgtacca tagagaacac ttagcctgtt taattgcttc
      361 aaggtaatca tgtgctttca gggctgctaa catcttacgg aatttcttca ccccgccaat
      421 acctaactgg aaggtcatga taataataaa atcattccag tcatcgggaa gatttaaccc
      481 taatgtctgt acctgcataa ttgttttggc taaatcgcga gatagtaact tgtctgcttc
      541 gctttcagta atgccagtac taaaatcttc tcccgcctga attaagtgac catatccaat
      601 agtactacat cctaaactat cagcatatgg atagaatttc ccatttctga agtacttcaa
      661 atttgcctga tactctttag taccttcata ttcttttaat cgtgttttta aatcgctcat
      721 ttactaaata cctataatga atttatagga atatttatga tgagtgaatg gaaatactcg
      781 gatgattgga gcgaggagga aatgttatcc ggtgaatatg ctggtttcgt ttatatcttt
      841 cagtttgagg atggttctaa ttatgtcggt gctaagcaaa tctatcagcg ggttaaggat
      901 gcgagaaagc taaagcccac ttcaaaggaa aatggctggc gtgagtacct cagtagttcc
      961 aatgtcgtta aaaacaaaat tgaaaatggt gaacagtact caaaaacgat tctatggtgc
     1021 ttcgcaacta tgcgagaggt aatgattgta gaggcaatac tgatccttca tcagatgctt
     1081 aaaccggaat gtttgaatct ggcaatgatg agtaagatta gatcaccaaa tgcaaaggat
     1141 aaaaaaagac tactcggaat agtacaggaa ctattggagt acttgaattg aggtaaatat
     1201 atggcaagtg gaataaatgg gattggtagt ataaggaact atatacaaac gcagggagtt
     1261 agattaaata atcaattcca gaatgaaata attaaacgtt ccaagctact atcccaaaga
     1321 atgcagggtg atttgaataa tagcgttgac cgtggggcag tgggctttac tcaacgttcc
     1381 atccttttct tctatagaaa ggttgggaga aattcagtta catcgaccat catggttaaa
     1441 gatattcagg ctaagtacct ttatcaagta ctggtacagc caaaggcaat tgataagttt
     1501 atacctactt catctacaag attaactaag cagggcaaca tttcaggact gaaaaagaat
     1561 cttttaagtg aacgatataa ggtagtgaag ggtaagaatg gtaaagaacg attaattgat
     1621 accgcaaaga aagataccaa gaagaaaacc aaacgtgtaa ttggcttacg tgagaagaaa
     1681 caacgtcgcc ttatctattg acttctataa ggaagccaat gatggtgtaa gggtaattat
     1741 gagcggtatt cagggaacat tcaggataag tagacaatga attttgagca acattatggt
     1801 gatttaataa gcaagattaa gctaaatggc atgacaccag atggtaatag tatgccaatt
     1861 aacaaatgtt ttcttgataa gaaatttcaa aaggtcgtta agaaaagtaa ttatcagatt
     1921 gatagctata tgaacgttat ttatgagaag aagaaagatt tcaaagttgg tgaagtactt
     1981 gaatgggaat tacagggtga aaagatgcct gtatttctaa ttgagagtaa aaagctcttt
     2041 gtgaagggta aacatttttg ggtatatgcc gtggggatta ttgaatgatt agtatattga
     2101 ttgagtttct gaagtcagga ttggattttt tcattaagaa gaaaactatt gaacaggaag
     2161 taaagaaaac gaatgtagaa gggcagattg aaacgaataa agaagagatt gaaaaggtaa
     2221 cattccactg gcgtaatgcc ctcggttttg tactaaccct tatcattctc tataactgga
     2281 tcatagtacc agtacttgat gcttttggaa ttattgtgat ccaagttcca ttaggacaac
     2341 ttttgcaagt gctattgatt atggttggtg gaagctgaaa gatttttcct ttaatatgtg
     2401 ttttgagtta ataaaatagc aatctaagga tttgatttat gagtgatgca attactacag
     2461 cagcaataaa aaaattgtat ggactatccg ctgggcaatg taatatttgc cgaacttctc
     2521 tttttaaaga gcaggttcac atcggacaaa tggctcatgt aatagccaaa agcccaaaag
     2581 gaccaagggg cgatgacctg ttagcaaatg acaattctta tgaaaatctt atcctgcttt
     2641 gtgcaaatga tcatattctt gttgatagag atccattcac ttatacagta gaaaaattgc
     2701 atcaaatcaa aagtgaacat gaggcttata tacagggtgc aaccgataat acattatttt
     2761 cagataaaaa aagacagtca gatgtggact ttttaaacgc ttacttccac tttacaccgt
     2821 ttgcgagggt acgttcatta gttgaaactt tacctaattc ttttcacatc aatcttctta
     2881 tttttggaga tcagtttgat agtattttaa gggattttcc agcgagctat cctttaaatg
     2941 ataaattact ccacggtcat ttccaaagtt ttattgattc gtataaaaat attgatttta
     3001 tcatatctgg acatttgccg atttccgata atagaaacat tcaggtgttt ggtggtgcga
     3061 atcataatct tctttgtcat ttcaattatg atgagctacc atccgataaa gcactgataa
     3121 tagaacaaga tttgatttca cataaaatat ctcttcttaa tgcttatgaa ggtcttataa
     3181 attaccttag agcttattac cctgaggtgg aaatttactc tcctattcta tgagtgttat
     3241 gaaatatata aaagcccctt atggggcttt ttttatggtt gttgtaatag taatggtttc
     3301 ttcgttttct ctaaaaaccg aatggctttg ttttattatt tccctaacat ctggtttctt
     3361 tatttttttg taatttaata aagccgaaac taaaacagaa acaaaaagta taagtgatac
     3421 tatacaaata gttaaacctt ctaaaggtga tagttggggg tctatatata atccatacat
     3481 agaaacagcg gagatagcaa taccgaaaac catccacata tctatagggc ttttatttgt
     3541 cgatttgacc gtagtgtact ttttgactaa gtccgtacct gatagcactg ttaaaattaa
     3601 tgataatata gcgagggcag tatttacatt aaaatttagc ttagcgaata aaagcaacat
     3661 tagtaaaata aaaataagta acgctatgat taaaaatatt cctttcttgt ccataaatac
     3721 cccgtcgtat ttttaaaaaa ggtatcttat ggcaaggata agtaaaaagg aatctgctaa
     3781 tcacaataaa gtgatggaat taatacattc agataagctc ctaacacaag aagataggga
     3841 attcatattc accaattata ggggggatgg gataggggct acaggtgcct tcttcacacc
     3901 tgaaatttta gcatgggatt ttgtacttga tgctgactgt actggtgact gtattgaact
     3961 atgtgctggt atcggtcgcc tcagctacta ccagtactta aggaataaac caacgcatat
     4021 tacatgcgtg gaactcaatc ctgagtacgt aatgatcggg aagagggtac taccagaggc
     4081 agaatggatc actggtaacg cactacagta ctcaccagac cgtttctatg atgttgccta
     4141 tggtaatcca ccctttggga agatcagtac ctcaaaagcg tatacaggcc gttataaggg
     4201 tagtgaattt gaatacaagg tgattgaaca tgcaagtacc tttgcgtcct atggtgcatg
     4261 gatagtaccg caaggttccg caggctttgt ctacagtggg gtacattgtt atgagcgtag
     4321 agaatcatgt aagtaccaga agtttgtgaa ggatactggc tatgagctac atccaggtgt
     4381 aggcatcgat acaagtatct acagggatga atggaatggt actaaggtga tatgcgaatc
     4441 ggtgttagta gatgatgacg gtacaaatct gtaactaaat tagttaagaa atcataaaga
     4501 tagtagtact tgcaaatgtt cccattgatg aagttatgcg tcctttcgtt ttttagcaca
     4561 ggttaagagc cgttcatatg aatagtagag gctcccggaa gaggtaaact gaacgacgtc
     4621 tcttgctaaa acacagcaac tctcatgcca ttttatcgac gatgagaaca acgtgcccgg
     4681 tgatacacct tcaaaattaa gcgtagatgg caaatggaaa acttgcctgc acggctttct
     4741 taatttcaat ggcatatcaa caactcatcc ataaattttg cataattaat gtaaagacaa
     4801 ggctcgccag taacgctaaa ttcatttggc tgtaagcgcg gtgtcatccg cgtcaggaaa
     4861 attaaacagt tactttaaaa aatgaaaacg taaaaaggtt gggtttcgat gtattgacgg
     4921 gtaaactttg tcgcccgcta aacatttgtt taaggaatga ttaattgtga ccaaactcaa
     4981 acttctggca cttggagtgc ttatcgcaac gtctgcaggc gtagcgcacg ctgaaggtaa
     5041 attttccctg ggcgcaggcg tcggtgtcgt tgaacaccca tataaagatt acgataccga
     5101 tgtttaccca gtaccggtaa tcaactatga aggcgataac ttctggttcc gtggcttagg
     5161 tggtggttac tacctgtgga atgacgcaac ggacaaactt tcaattaccg cttattggtc
     5221 gccgctttac ttcaaagcga aagacagtgg cgatcaccaa atgcgtcacc tggatgaccg
     5281 taagagcacc atgatggctg gtctgtctta tgctcacttt acccagtacg gttacctgcg
     5341 taccaccctg gctggcgata ccctggataa cagcaacggc attgtctggg atatggcctg
     5401 gttgtatcgt tacaccaacg gcggcctgac agtgaccccg ggtatcggtg tgcagtggaa
     5461 cagcgaaaac cagaacgaat actattatgg cgtatcgcgc aaagagtccg ctcgcagcgg
     5521 tctgcgtggc tataacccga acgacagctg gagcccttac ctggagctga gcgccagcta
     5581 caacttcctc ggcgactgga gtgtttacgg taccgcgcgc tatacccgtc tgtctgatga
     5641 agttactgac agcccgatgg tggataaatc ctggactggc ctgatttcta ccgggatcac
     5701 ctacaaattc tgataatgca tctttccagg gcattttgtg cataaaaaca gggcgtcacg
     5761 ccctgttttg cattacggtg gtgcaatacc tgggaggaaa caatgcaaca aaaaatgatt
     5821 caatttagtg gcgatgtctc actgccagcc atagggcagg gaacatggta tatgggcgaa
     5881 gatgccagtc agcgcaaaac agaagttgct gcactacgcg cgggcattga gctcggttta
     5941 accctcattg ataccgccga aatgtatgcc gatggcggtg ccgaaaaggt ggttggggaa
     6001 gcattaaccg gtctgcgtga taacgtcttt ctcgtctcta aagtctatcc gtggaatgct
     6061 ggcgggcaaa aagcgataaa tgcatgcgaa gccagtttac gccgtctcaa tactgattat
     6121 ctcgatctct acttattaca caggtctggc agtttcgctt ttgaagagac tgttgcagcg
     6181 atggaaaaat tgatcgccca gggaaaaatc cgccgctggg gcgtttctaa ccttgattat
     6241 gctgatatgc aggaactctg gcagctgccg gggggaaatc agtgtgccac taatcaggtg
     6301 ctttaccatc tcggttcacg agggattgag tacgatctac tcccctggtg ccagcaacag
     6361 cagatgccgg tgatggctta cagtccatta gcccaggccg ggcggttgcg caatggactg
     6421 ttaaaaaacg cggtagtcaa cgaaattgca catgctcaca atatcagctc ggcacaagta
     6481 ttgttggcgt gggtaatcag ccatcagggt gtgatggcga ttccaaaagc ggccacgatt
     6541 gcccatgttc aacagaatgc ggctgtgctt gaggtcgaac tttcgtcagc ggaattaacc
     6601 atgctggata aagcgtatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt
     6661 tgatacgtgc cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttcgca
     6721 acgcgaatgg attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa
     6781 gcgtaagccg tttctacaca aacaaatgtt ttgtagccat catccggcat atcgcccatg
     6841 ctaattgaaa gcgccggtcc cgggttccag cctacaacgt tcagatgatg ctggtggcct
     6901 acggcgataa tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga
     6961 tacacgcgat cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc
     7021 gcgtcattca ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata
     7081 tcacccacgt taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca
     7141 agatcgattt cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcgga
     7201 cagaattttt tcgtctcttc gctttgcgtc aattcaaaag tcagcgctac gccatcagca
     7261 tcttcattat gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga
     7321 ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg
     7381 acgccatttt tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt
     7441 ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg cttttacttg gggatgatcg
     7501 accacaatga ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg
     7561 atgaccggaa gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca
     7621 ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca cagtttcatc tcaacttatt
     7681 tggagatgtg agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc
     7741 aggataccag tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg
     7801 aagtgcaaac ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa
     7861 cgcctttcat ttcgccttca gcagcggctt taacggcagc tttgatctgc tcgtaagttg
     7921 cagctttttc cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga
     7981 acgccatacc agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag
     8041 caccggtaga ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt
     8101 gagacgggcc atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac
     8161 cttcgatgat gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg
     8221 tgcaggaagc gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa
     8281 cgaacatcgg agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg
     8341 tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt
     8401 caacaccaac ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt
     8461 ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt
     8521 gagtggagtc atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg
     8581 caacgatctc gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc
     8641 caaaaccgtt gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata
     8701 aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca
     8761 ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg actcgcctca ctcttccttt
     8821 gggcttgaaa ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg
     8881 tgattttcat cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt
     8941 tgccagttgg tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg
     9001 agatgtgagc aaatggctaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag
     9061 atgcagtttt acgtgacgca gaatcatggg acagagccgc catttacggg tcgtttactg
     9121 cataacaagc gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc
     9181 caaaccaagt atgattccgg ctgtggttgg cccagtttct acgaaccggt gagtgaagaa
     9241 tccattcgtt atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt
     9301 aactgtgatg cccatctggg gcatgtcttc cctgacgggc cgcagccaac gggcgaacgt
     9361 tattgtgtta actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac
     9421 ggttgaaaaa acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg
     9481 acattatcaa cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg
     9541 ggaaatggcc cgatggcgtt gcgttgaccg aggaacaaaa agcgaactgc ctgcaactgg
     9601 tgatgctgtg gcaagctcgc cataataccg aagcacagca tatgacaatt gacacaaatg
     9661 gtcagatggt catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc
     9721 ctattgctat gtttaagtga atgagttatc tagtatttaa gcctgttatt aagggagggc
     9781 aaatatactt gttaggacgt cgtgttttag ctgcaagtag gttaaatgca acactgacaa
     9841 gtattatctg gcctacaatg tcattgagga aatgtaggcc agagatgaat tcaactaata
     9901 ttacccctgt gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat
     9961 ctccataaac gcgtgcgcac tgtcctgggg ctggatattc acgccacgac aaccatcggt
    10021 aatcacattt accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata
    10081 gtcagtagcc aggcccataa cgatcaattc atcgatttca tgatcgcgta accagtcatc
    10141 gagcgaggtt ttctgccgac ggccgttatc aaaaaaggca ctgtaactgt caactaaagg
    10201 attttcgcct ttatggaaca ccgctgcgat cgctttttgg ttcagtaacg gatgtaattg
    10261 tgcgccttca ctgttctgca cacagtgatc tggccagaag gtttgtggca aaccgtcgag
    10321 ttggcctggc gtataaggct ctacaccgtg ctgactggca aaactgccgt gattcgccgg
    10381 gtgccagtcc tgactggcga taaccgcttc accgcgcgac tggcaccagt caatcaggcg
    10441 gttagcgaca tccaccgtac tgtcaccttc cggcacggcg agcgcgccac cagcacagaa
    10501 atcattttgt aaatcgacca gtaacagggc gcgagggggc attacagtct ccttaatcat
    10561 ccggcgtcag ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca
    10621 gttcctggct cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac
    10681 cgccaataac gccggcatgg gcgagggcgt taccggtggc ataaccaccc atattcactt
    10741 taccggacat acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta
    10801 attcctgcag gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca
    10861 atgctttcac cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa
    10921 tcgtcactac gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt
    10981 gcggggcggg tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa
    11041 ggtttggaga ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc
    11101 gatacaatcg gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt
    11161 acaacgcatt cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg
    11221 accctgtcac aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcag
    11281 tatacgccat cgtgtcggtg ccgtgcagga tgacaaaacc atcatagtcg tcatagtgcg
    11341 ctttaatatc ttcagcaata tgctgccagt cttctggcgt catatcagaa gaatccatca
    11401 gcggcgtata ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca
    11461 gcgccagttg gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca
    11521 tcccgatggt cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat
    11581 gaatgatatc gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat
    11641 gcggataagg aaatatgggg cgagacagcg ggaagaggat tcgtcgacgt aaaaaataat
    11701 gtggccgacg atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg
    11761 caaaacgcat aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta
    11821 acggtagagg ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac
    11881 attgcccgga cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca
    11941 acgtagtatt ccagatgcca ctgtttcact tttgccagct ctgctgcttt ggcgaccgca
    12001 tcatcgaaat ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag
    12061 acgtggccct gggcgatttt atcaatctgc tccggcgtcg aatgacgcgc atcagcaacc
    12121 agcgtgataa agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc
    12181 ggcggcagtg ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta
    12241 tgaacaccaa tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata
    12301 gaaccggtca gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa
    12361 ccaccagatg ccgccatgcc gcccatcgat acaaccacag gcttacccgc tgcccgggct
    12421 gctgccagtt cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca
    12481 cgcaggacaa tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg
    12541 gtatcaccgc caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag
    12601 acgacaccga tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg
    12661 atggcgcgat aatttttatc agtcttactc cagccgaact ctttggttag agctttttcg
    12721 atttcggcac tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta
    12781 tcgccaccgg ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga
    12841 gcagggatct gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc
    12901 cagcggctgt cagcttcgcg ggctgccggt gacatgtcat cacgaataaa cggttcaacg
    12961 gcagatttat acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac
    13021 gatttgtagt acagaccgtt ggtagcaaaa ccgtgcaaat ccaccacgcc ttgcggtgac
    13081 agccaaattt tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca
    13141 acggcataaa ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc
    13201 atagacggct ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg
    13261 cggtcgtcct tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga
    13321 cgatcggaac tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtcg
    13381 ggtttatcga cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct
    13441 ttcgaatcac caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata
    13501 aagaacaggt taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa
    13561 aatccggcaa taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg
    13621 tcacaggcgc aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt
    13681 gcggactgtg ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca
    13741 ggagttaatc aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg
    13801 gctgaacccg cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg
    13861 ccggaccata agtccatgca accgtggcat ttttttgtga ttgaagggga agggcgcgag
    13921 cgtttcagcg ccgtactgga acagggggcg attgctgccg gtagtgatga caaagctatc
    13981 gacaaagccc gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc
    14041 gaagaaaatc ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg
    14101 gcgatgcaaa tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta
    14161 actgaaagtc cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt
    14221 tttctctacc tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg
    14281 acgccgtttg taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg
    14341 cgcaggaatt cagacactct cacttatcac ttcacggaat gagggttacc atacccggat
    14401 tgcattgaca ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg
    14461 gagctggttg cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc
    14521 aggcgaagtt tgttgatccg aatttgcttg tgggtaatga gacccgcgac gatgcggcgg
    14581 tgtacgatct gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg
    14641 ttgataatcc tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg
    14701 cgatgggggg caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt
    14761 ccccggaaat tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta
    14821 ttgcgctggc tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa
    14881 cggggatcgt accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac
    14941 tgttcctgac gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgc
    15001 tgaaaccaga acatcaggga ctagcgacgg aagtgatgtg ccggatgaac atcgcaggcg
    15061 cgtcctttgc caacatcgaa ggcgtaaaag cgatgaccga cgttacgggt tttggtctgc
    15121 tgggccattt gagcgaaatg tgtcagggcg caggtgtgca ggcgcgcgtt gactatgacg
    15181 cgatcccaaa attgccgggg gttgaagagt acattaagtt gggcgcagtg cctggcggca
    15241 ccgaacgtaa ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg
    15301 atttgctgtg cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag
    15361 aaaatgaggt caaagctaca gccgccgagt ttggcattga actgacggca attggcgaac
    15421 tggtgccagc gcgcggcggt cgtgccatgg ttgagattcg ttaattcaat gcggttgttt
    15481 attgccgaaa aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg
    15541 aaaggcgatg gctttatcga gtgcggtaat ggtcaggtgg tgacctggtg tatcggtcac
    15601 ctgcttgagc aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg
    15661 gatttgccga ttgtcccgga aaagtggcaa ttacagcccc gaccctccgt gaccaaacaa
    15721 cttaacgtca tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg
    15781 gatcgtgaag ggcaactgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa
    15841 aagcgccagc aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg
    15901 gcgctcgacc gtcttcggtc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg
    15961 cgagcgcgtg ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt
    16021 cgcaatgccg gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg
    16081 ctggtggtgc gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc
    16141 aaagcacata tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa
    16201 gcgtgtgaac cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat
    16261 gtggttaacc gcattagtgg tcaaccagca attgtcacca gctataacga taaacgggaa
    16321 tcagaatccg cgccgctgcc tttttcgctt tcggcgttgc agattgaagc ggcaaaacgc
    16381 tttggtctga gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag
    16441 ctaatcactt atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc
    16501 cacgcggtga tgaatgccat cagcgttcat gcaccagatc tgttgctgca gccagtggta
    16561 gatccagata tacgcaaccg ctgttgggat gacaaaaagg tcgatgcaca ccacgccatc
    16621 atcccgaccg cacggagttc agccatcaac ctgacggaga acgaagcgaa ggtctataac
    16681 ctgattgccc gtcagtactt gatgcagttc tgcccggatg tggtgttccg caagtgtgtt
    16741 atcgaactgg acattgccaa aggcaaattt gtcgctaaag cgcgttttct tgctgaagca
    16801 ggctggcgca cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg tacgccattg
    16861 cctgtggtgg cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa
    16921 acccagccgc cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg
    16981 cgctttgtgc aggacaaaga tctgaaaaag atccttcgtg cgaccgatgg tctggggaca
    17041 gaagcaaccc gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa
    17101 gggcgctata tccactctac cgacgccgga aaagcgttat tccattcgct gccagaaatg
    17161 gcgacgcgac cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag
    17221 cagtgtcgct atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat
    17281 caagccaaac gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc
    17341 agtgctgata agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca
    17401 gatgaagtcg gaagcggggc gatagcgtaa gcgagtgaat ctttcgtgct attcgag
//
LOCUS       DAZRVY010000078        17447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZRVY010000078 DAZRVY010000000
VERSION     DAZRVY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.078"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHXOX_002736"
     CDS             complement(<1..501)
                     /locus_tag="ACHXOX_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7006159.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHXOX_002737"
     tRNA            complement(687..771)
                     /locus_tag="ACHXOX_002737"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHXOX_002738"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHXOX_002738"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR7006160.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHXOX_002739"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHXOX_002739"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR7006161.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHXOX_002740"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHXOX_002740"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR7006162.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHXOX_002741"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHXOX_002741"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR7006163.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4654..5973
                     /gene="idnT"
                     /locus_tag="ACHXOX_002742"
     CDS             4654..5973
                     /gene="idnT"
                     /locus_tag="ACHXOX_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR7006164.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6040..7038
                     /gene="idnR"
                     /locus_tag="ACHXOX_002743"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6040..7038
                     /gene="idnR"
                     /locus_tag="ACHXOX_002743"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR7006165.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7116..8618
                     /locus_tag="ACHXOX_002744"
     CDS             7116..8618
                     /locus_tag="ACHXOX_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR7006166.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHXOX_002745"
     CDS             complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHXOX_002745"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR7006167.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHXOX_002746"
     CDS             complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHXOX_002746"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR7006168.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHXOX_002747"
     CDS             complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHXOX_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR7006169.1"
                     /translation="MSIIILATISTTQENK"
     gene            11186..12697
                     /gene="pepA"
                     /locus_tag="ACHXOX_002748"
     CDS             11186..12697
                     /gene="pepA"
                     /locus_tag="ACHXOX_002748"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR7006170.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12831..13274
                     /gene="holC"
                     /locus_tag="ACHXOX_002749"
     CDS             12831..13274
                     /gene="holC"
                     /locus_tag="ACHXOX_002749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR7006171.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13274..16129
                     /gene="valS"
                     /locus_tag="ACHXOX_002750"
     CDS             13274..16129
                     /gene="valS"
                     /locus_tag="ACHXOX_002750"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR7006172.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16185..17381)
                     /locus_tag="ACHXOX_002751"
     CDS             complement(16185..17381)
                     /locus_tag="ACHXOX_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR7006173.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4174 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacgtgcag cgcgaaagcg
     4621 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4681 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4741 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4801 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4861 atgttaggca agctgatttc tgatacgggc gcggcacaac gtatcgccac tacgctgatt
     4921 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4981 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgtg
     5041 gcatcatctg gattacccct gttgtatgtt ggcgtaccga tggtagcagc gctctctgta
     5101 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5161 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5221 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5281 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat ttttgctgca
     5341 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5401 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5461 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5521 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5581 ggcgcgttta agcaggtatt agtagatagt ggtgtcgggc agtatatttc acacttaatg
     5641 accggaacta cgctttcgcc gttattgatg tgctggactg tcgcggcgct gttgcgtatc
     5701 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgattatc
     5761 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ctggtgcggg cagcgtgatc
     5821 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5881 ggtgaaacgt tgcgtacctg gacagtgatg gaaaccctta tttctattat gggtttgctg
     5941 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     6001 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     6061 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6121 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6181 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6241 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6301 gtcacctctg tgcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6361 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat tctttcggaa
     6421 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6481 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggct
     6541 gcttttgaca tggtgtgtac catgctggat aagcgagtca ggcgcaaaat tctgtatctc
     6601 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6661 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6721 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6781 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6841 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6901 ctcgccagcg taattactcc ccgttttgat attggtcgga tggccgccca aatgttgctg
     6961 agcaagatta agaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     7021 cacggtaaca cgctttaggt tattttctct tcattacaca tattctcttt gccggacgtt
     7081 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7141 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     7201 ctggcgcaac ggggacgggt aaaactgtca cgttgcaaaa actggcagag tcattgtcgg
     7261 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc atcgcgcagg
     7321 caggaacggc gtcggaaaaa ctgctcgcaa gactgaaaaa tatcggcgtc aacgactggc
     7381 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7441 gggcgacggt ttcggatctg gggccgctgt tgctggcacg actgttgaat ctcaacgatg
     7501 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     7561 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     7621 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     7681 cgctggaaca gcaaggcgca gaacacttct ttggtgagcc gatgctggat atcaaagact
     7741 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7801 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttatatgaac
     7861 aattgcctga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaagcac
     7921 atctgctgtt taacgatgcg ccacaggtac tgctggataa gattgagcag gtgataaggc
     7981 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtca gatatcccgg
     8041 ataacgtgct agggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccta
     8101 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcgtttgata
     8161 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcga
     8221 aaggaagtcc ttcagtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     8281 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     8341 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     8401 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     8461 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     8521 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8581 ggatgttggg gagtttgctg ggggggagaa aaaggtaagt cagacgtgat ctgtgcccct
     8641 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     8701 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     8761 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     8821 ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     8881 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     8941 cagcgccatc aacatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     9001 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat agttgtgcaa
     9061 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     9121 gttggttttc caggtgccgc tcaccgtctg cgaaccggtg atctgtttcg gattggtcag
     9181 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     9241 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     9301 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     9361 ccataagcct tgctgagtgg agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     9421 acgcgccatc tgctcgccct gcgacgcgac ccattcacca atcgccatcg tcagcaagac
     9481 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     9541 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     9601 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     9661 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     9721 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     9781 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     9841 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     9901 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     9961 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
    10021 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
    10081 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgggcacga
    10141 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
    10201 accaccgcct ggtgaccaat gatcgcctga taatcctgaa agtccgtaat gcggaaatcg
    10261 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
    10321 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
    10381 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
    10441 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
    10501 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccatggtccc
    10561 gcccacatca cgttaaccgc cgcgacgatt gccgtgaaca ccgcaaggat cattgccgct
    10621 ttcaccagca ccgctttgct cagaccgcag gcatgcatta ccgtaatttc actttcggta
    10681 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
    10741 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
    10801 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
    10861 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
    10921 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
    10981 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
    11041 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
    11101 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
    11161 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
    11221 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
    11281 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
    11341 ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt ccgaatgtac tttccgagcg
    11401 aattctcctt attggttgcg gcaaagaacg tgagctggat gaacgtcagt acaagcaggt
    11461 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
    11521 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
    11581 tgcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
    11641 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
    11701 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
    11761 tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc agctggctga
    11821 cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga aagagctggg
    11881 gatgcactct tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga tgtcggtgat
    11941 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
    12001 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
    12061 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcag agctacaact
    12121 gcctattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
    12181 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
    12241 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
    12301 agcggtgatt gatgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
    12361 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
    12421 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
    12481 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
    12541 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg caggaaccgc
    12601 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
    12661 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
    12721 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
    12781 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
    12841 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
    12901 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    12961 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    13021 ttgtcccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    13081 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    13141 cagattttgc cactgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    13201 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    13261 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    13321 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    13381 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    13441 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctatggca
    13501 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    13561 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    13621 atggaaagcg gagtccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactctgt
    13681 cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt
    13741 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
    13801 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    13861 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    13921 tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg tggccgttaa
    13981 cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac cgctggttaa
    14041 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    14101 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    14161 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac
    14221 caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga
    14281 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcactcggcc tgctggaaga
    14341 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtag ttatcgaacc
    14401 aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cggttgaagc
    14461 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    14521 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    14581 tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa
    14641 ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    14701 gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ctgacgcact
    14761 gcgccagttc cacccaacca gcgtgatggt atccggcttc gacatcattt tcttctggat
    14821 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    14881 gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    14941 caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccgga
    15001 actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    15061 gcgcaccgag aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt
    15121 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    15181 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    15241 ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg cggaccgctg
    15301 gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    15361 cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    15421 tctggagctg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    15481 ccatacgctg gtgaccgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    15541 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    15601 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    15661 caccgaatgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaatat
    15721 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    15781 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    15841 gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct
    15901 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    15961 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    16021 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    16081 agcgaaagcg aaactgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    16141 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    16201 gaaggcataa ttccccgtga aatactggct aagaatcctt tatctggttg ttcttcttta
    16261 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    16321 atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga tatcaccacc
    16381 aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa acgaatacgc
    16441 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    16501 gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt acgttgcatt
    16561 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    16621 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagccaaa
    16681 aatgcgagaa tggcatattt tatacaatat gtagtatcta atttcacctt gaaacgatgt
    16741 ataccgaaac tggtattact ccagagaaaa ctcattacca gactatataa agtgccatta
    16801 aaaataccaa tgaaaacaat acaaactatt gtcatcagaa ccacagatat aataacgcta
    16861 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaagg
    16921 gccattaaaa tcggcaagaa aaaggtcacc caccagaacc ctttcataga gcaattaaaa
    16981 ctaaatctta cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc
    17041 ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaataag cggcatatct
    17101 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    17161 acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat attagcatag
    17221 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    17281 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac
    17341 gaatgggacg gaacatccat atcattaata acttgagcca taaaagttcc ctgaattaaa
    17401 aaatccattg atataattta tatatacaga cagtaaaatg ttctgcc
//
LOCUS       DAZRVY010000079        17422 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZRVY010000079 DAZRVY010000000
VERSION     DAZRVY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.079"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHXOX_002752"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHXOX_002752"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR7006174.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHXOX_002753"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHXOX_002753"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR7006175.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLTQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHXOX_002754"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHXOX_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR7006176.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHXOX_002755"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHXOX_002755"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR7006177.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHXOX_002756"
     CDS             complement(5488..5685)
                     /locus_tag="ACHXOX_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR7006178.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHXOX_002757"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHXOX_002757"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR7006179.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHXOX_002758"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHXOX_002758"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR7006180.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHXOX_002759"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHXOX_002759"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR7006181.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHXOX_002760"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHXOX_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR7006182.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHXOX_002761"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHXOX_002761"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR7006183.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHXOX_002762"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHXOX_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR7006184.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHXOX_002763"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHXOX_002763"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR7006185.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHXOX_002764"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHXOX_002764"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR7006186.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHXOX_002765"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHXOX_002765"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR7006187.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHXOX_002766"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHXOX_002766"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR7006188.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHXOX_002767"
     CDS             complement(17127..17180)
                     /locus_tag="ACHXOX_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006189.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3947 a         4973 c         4376 g         4126 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gtcagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg gg
//
LOCUS       DAZRVY010000080        17339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZRVY010000080 DAZRVY010000000
VERSION     DAZRVY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.080"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            93..1133
                     /locus_tag="ACHXOX_002768"
     CDS             93..1133
                     /locus_tag="ACHXOX_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR7006190.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHXOX_002769"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHXOX_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR7006191.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHXOX_002770"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHXOX_002770"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR7006192.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="ACHXOX_002771"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="ACHXOX_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR7006193.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHXOX_002772"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHXOX_002772"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR7006194.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="ACHXOX_002773"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="ACHXOX_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR7006195.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACHXOX_002774"
     CDS             complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACHXOX_002774"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR7006196.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9263..10681)
                     /gene="araE"
                     /locus_tag="ACHXOX_002775"
     CDS             complement(9263..10681)
                     /gene="araE"
                     /locus_tag="ACHXOX_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR7006197.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10996..11757)
                     /gene="kduD"
                     /locus_tag="ACHXOX_002776"
     CDS             complement(10996..11757)
                     /gene="kduD"
                     /locus_tag="ACHXOX_002776"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR7006198.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11822..12658)
                     /gene="kduI"
                     /locus_tag="ACHXOX_002777"
     CDS             complement(11822..12658)
                     /gene="kduI"
                     /locus_tag="ACHXOX_002777"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR7006199.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12945..14126)
                     /locus_tag="ACHXOX_002778"
     CDS             complement(12945..14126)
                     /locus_tag="ACHXOX_002778"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR7006200.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14381..15610
                     /locus_tag="ACHXOX_002779"
     CDS             14381..15610
                     /locus_tag="ACHXOX_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7006201.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16070..16702
                     /locus_tag="ACHXOX_002780"
     CDS             16070..16702
                     /locus_tag="ACHXOX_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7006202.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17036..>17339
                     /locus_tag="ACHXOX_002781"
     CDS             17036..>17339
                     /locus_tag="ACHXOX_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7006203.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4596 a         4523 c         3989 g         4231 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
     8881 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
     8941 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
     9001 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
     9061 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
     9121 aattgttttc attcctctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac
     9181 gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag ggccggatgt
     9241 acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg cctgccatca
     9301 gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga
     9361 tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcaccaatgc
     9421 tgtcgagcag cgtcaggaag gtcgcgccga taatcatatt cgacacccag ttcgtggtgg
     9481 tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag cacaggatcc
     9541 acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag
     9601 agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg caatagccca
     9661 gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt ttacgccctg
     9721 ctttatctac cgtaaacacc gcaataaagg tggcgaacat aaaggtcagc cctacgacca
     9781 gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgcggcg
     9841 cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga
     9901 gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta
     9961 acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca
    10021 gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc cagcgcgggc
    10081 tatttggcag gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca
    10141 acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga
    10201 tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg
    10261 ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca
    10321 ccacccgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct atagaaccga
    10381 gtacaaacag gatggccccc gccatcaggc tgtatttacg ccccaggcgg aacgacagcc
    10441 aaccattaaa cagcgcacca attgctgcgc cgagcatcat gctactaacc acccattcct
    10501 gcaaacgact ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc
    10561 cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca
    10621 tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca
    10681 ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ctcacacagg
    10741 tgtcaggtcg gaaacagcat aaatatggat taaattgctg cgacatgtcg ttatgtgatg
    10801 gatattccaa ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta
    10861 cagcagataa aattcataaa gttcattaat tgataattga tatggattat ttcataacca
    10921 tgatatgaat tatgatgacc tacaggtata aaaaaccctg ccatgaggca gggtcataaa
    10981 agtaagagga atgaattaac gagccagcca accgccatct acggcaatgg tataaccatt
    11041 cacataatct gaagcgctgg aggcaaggaa cactatcggc cccatcaggt cactcggcag
    11101 tccccaacga ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg
    11161 tagttgttga gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt
    11221 gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc
    11281 ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgtga tattgataat
    11341 cttgccgcca ttgcgttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac
    11401 gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcatc
    11461 ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac
    11521 cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa
    11581 aaaacgacgc cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat
    11641 gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagtccagt
    11701 atcacaacca gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac
    11761 aataccttat tcgtttatgc cgacaactag cgcaaatctt taacggccac tatgccgaca
    11821 actagcgcaa atctttaacg gccacatggt ccatatcatc aaagacctgg ttttcaccaa
    11881 ccatgcccca gataaaggta taagctttgg ttccgacgcc ggaatggatc gaccagctgg
    11941 gggagatcac cgcctgctcg ttatgcatca caatgtgacg cgtttcttgc ggctgcccca
    12001 tcatgtggaa aacgcaggcg tcatcatcca tattgaaata gaaataaact tccatccggc
    12061 gctcgtgagt gtgacacggc atggtgttcc acaagttacc tggagccagc tccgtcagcc
    12121 ccatactcaa ttggcaggtt tccagcacat ccgggacaaa atatttgtta attgtgcgac
    12181 ggttactggt gaggttatcg cctaacgtga ctggagatac ttcgtccggt gtgacttttt
    12241 tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata aaactttgcc ggagtggcgg
    12301 tatcaatact ggcaaagaca acttcttttg cacctttacc aacatacagg gcgtcgcggt
    12361 gaccgatttc atagcattgg ccatcgacag taatcgtacc ggcaccgcca atattgataa
    12421 cacctaactc gcgacgttca aggaaatagc ttacgccgag ttgtttacca acttccccgc
    12481 caacggaaac cgttttagtt atcggcataa tgccgccaac aataattcgg tcaatgtggc
    12541 tgtaaaccat ggtgtactca tcggcgacaa ataccttttc aaccaaaaat tcattgcgca
    12601 gcccttgggt atccagcgtt tttgcgtgcg cactgtggat gccctgtctt acgtccacat
    12661 caacctccga taatcagtga agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt
    12721 ttctctataa agggatgata tgcccatggg ctagggtttt caattaaaaa taaaacaatg
    12781 tttcattttt aagttaagga ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga
    12841 atggaaaaat gagggagccc agaaaatgta tgtcgacttg ccagccatca ttattcaaca
    12901 gattaatgac ggcagatatt tatcggtaag gaggaaatgc agagctactc gtcacgttca
    12961 atggtcaatg ccacgccctg gcccccgccg atacaaagcg ttgccagccc tttgcgggca
    13021 ttacgtttca ccatttcatg aaccagagaa accaggattc ggcaaccgga agcgcctatc
    13081 gggtgaccga gtgcgatcgc gccaccattg acattgaccc gacgctcatc ccactcaagc
    13141 atcttgccaa ccgaaagcgc ctgtgcagca aacgcttcat tagcctcgat aagatcgaca
    13201 tcagccaact gccagcctac acgctccagg caacggcggg tcgcatacac cggcgcaatt
    13261 cccatcaatg ccggatctac accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt
    13321 aaattcaacg ctcgtgcttt ggcttcgctc atcatcatta ccgcagctgc gccatcgttt
    13381 atggatgatg cattccccgc ggtcacagaa ccgagactat caaatgaagg atttaaacgg
    13441 gctaaacctt ctgcactggc gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc
    13501 tgaccgttac tttggatcat taccgggacg atctcatctt taaatcgtcc ggcgtcaatc
    13561 gccgctcgcg ctttttgttg cgagctaagt gcgtaagcat cctgcaactg acggctgatg
    13621 ccatattcgc gagccagatt ttcggcggtg acaccaatat gataatcatt gaaggcatcc
    13681 cacaacccat catgcaccag actgtcaacc aactggctat tgccaagctg tgcaccggtg
    13741 cggctatcag tcagaacatg tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca
    13801 atatcagcct cgccacactg tatcgcctga gtagccagat gcagtgcttt aagtccggaa
    13861 ccgcaaacgt cattaatcgt gattgcagaa acgctattag gaagaccacc tttaatagcc
    13921 gattgccttg ccggattctg ccctgcccct gcagtcaaca cctgaccaag aattacttca
    13981 tccaccgcat atgcaggaac gccggtacgt tctattaacg ctttcacgac cagactacca
    14041 agttccacgg cggaatgacc cgctaacgca ccacgaaagc agccgatagg tgtccgtaac
    14101 gccccgacaa tcacaacgtc tttcatcaca acctcgcacc aattaacagt gcaatagtaa
    14161 atgattgtta attacagtta tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa
    14221 atatgcctga gcagtagtca gagtcataac tggcacgtaa ggtttgcaac cactaaccca
    14281 ccaatagatg ggtagataag gcgttaatct cccatactta acctggttta tggtaaattg
    14341 ccctccattt tgtttaattt gtagatgata cgttcagata atgtctaata tttggtctaa
    14401 agaagaaact ctgtggagtt tcgcgctcta cggcacagcc gttggtgcag gcacgctctt
    14461 ccttcctatt cagttaggtt cggcaggggc tgtggtcctg tttattactg ctctggtcgc
    14521 ctggccttta acatattggc cacataaagc cttatgccag ttcatcctct catcaaaaac
    14581 atcagcatgt gaagggataa cgggcgcggt aacacactac tatggcaaga agattggtaa
    14641 tctgattacc acgctgtact tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc
    14701 aattaccaac tcacttacgg aacagctggc aaagcatatg gttattgatc ttcgcatccg
    14761 tatgttggtg agtctgggtg ttgtattaat tctgaatctc atttttctga tgggacgtca
    14821 tgccactatt cgggtaatgg gatttttggt attcccattg attgcctatt tcttatttct
    14881 ttccatttac cttgtcggta gttggcaacc tgatctatta acgacccagg tagagttcaa
    14941 tcagaatacc cttcaccaga tatggatatc gattcccgtg atggttttcg cctttagcca
    15001 tacgcccatt atttctacgt ttgccataga cagacgtgaa aaatatggcg aacacgctat
    15061 ggataaatgc aaaaaaatta tgaaagtcgc ttatctcatc atctgcataa gtgtactgtt
    15121 ctttgtcttt agctgcctgc tttctattcc accttcgtat attgaagcgg ctaaagaaga
    15181 aggggtcacc attttatcgg cgctttctat gctgccgaac gccccagcat ggttgtcaat
    15241 ttccgggatt attgtcgcag tagttgcgat gtcgaaatca ttcctgggta cgtactttgg
    15301 cgttattgaa ggtgccacgg aggtcgtcaa aacaacacta cagcaggttg gtgtaaagaa
    15361 aagtcgtgca tttaaccgcg cactatcaat tatgttggta tcgctgatta ccttcattgt
    15421 ttgttgcatt aacccgaacg cgatttcgat gatttatgcg atcagcggcc cgctcattgc
    15481 catgatactt ttcatcatgc ctacgttgtc aacgtatctg atcccggcgc ttaaaccctg
    15541 gcgctccatc ggaaatctga ttacgctgat cgtgggtatc ctgtgcgtat cggtaatgtt
    15601 ctttagctaa tatccgttgt ggatggttca aaattgctac aattttgaac cattccattt
    15661 ttcataatat tcatttgtta ttattccgcc ctatcacttc tctcgtcata tcctagaatt
    15721 atcagttagt taaacacaca aagagatctg aacatcttaa ataagactat ttaagatgca
    15781 taacttagat tcgcaagata tcttctggtc attcaaaaac aatttgcgat atagatattc
    15841 tcataccatg agatagttat ctggaccacg ttcgaccatg caagattaaa tcagtgaaac
    15901 ataatcatat ttaataacga gaaatgcatt tttaaatgtc ttaatcagaa ttatagcaaa
    15961 tacagattaa tcgcaatttt tcccagcaaa attacaaaat caagatgtat tgacatcatc
    16021 aatcaccaat gaaattatgc aattatatac ggatagggag gttcttaaca tggggctttg
    16081 tagtcgttat aaaagtctta catgcaatag ttgctcaatg cattgccaaa taatgccaga
    16141 agagtcaccg cgtttgcagt attgcgcaaa ctcgtgtttt tgtatgtggc ccgaagaaag
    16201 ttcatatttt aatcgtggtg tggtggaagg tatcttaaca aaaaaccaca atgcgagatt
    16261 aagcggatat atttttgtgg atttttcagt aagtttttta cgtctattcc ttgaaaaaga
    16321 ctggattgac tatcttgcaa gtactgacat ggggatagtt ttagtcagcg accgcaatat
    16381 gcaatcgtta gctaactact ggcgaaaaca caacaccgca atatctgctg tcatatataa
    16441 tgatgatggt cttgatgttg ccaatgaaaa gatcagacaa ctatttattg gtcgttattt
    16501 atcatttacc agaggtaaca cattaacaca gatggaattc accataatgg ggtatatggt
    16561 ttctggttat aatccatatc agattgctga agtgctggat atggatatcc gtagcatcta
    16621 cgcgtacaag caacgaatcg aaaagagaat gggtggtaaa ataaacgaat tatttattcg
    16681 ttcacattcg gtccaacatt gatactcaac gaccagccag aatcatactc tgttcatacg
    16741 ggcatctatt gaaatgcccg tattttataa ataagcagta accccaagag taaacaacat
    16801 agtatttaaa accatcacac gaaaaattca gaagcattac gaaatatgaa tttacaaaca
    16861 atagtggcat aaatgttaac catgttaatt tacgtaaagt tttacgttgc aacattaaag
    16921 cctcatttca atcatcatga taaatataaa attaatatat atttatgccg taaataccaa
    16981 tatacttagc aaactatgtg atctccattt cgattgattt agtgtttatt gacgtatgta
    17041 ctggattatt aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca
    17101 taacgccgat cttaacgtcg ttctgacaac gccagccagt cgatgtttat cacttctact
    17161 tgaagctttt cctgatgtgg ttacacaaca agattttttc accagagtct gggaagaaga
    17221 aggtatgcgt gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt
    17281 tcgcgctgtt ggtgatacta cccactcgct aattgcaacc gtgccgagag aggattcaa
//
LOCUS       DAZRVY010000081        17115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZRVY010000081 DAZRVY010000000
VERSION     DAZRVY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.081"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            98..523
                     /gene="nudI"
                     /locus_tag="ACHXOX_002782"
     CDS             98..523
                     /gene="nudI"
                     /locus_tag="ACHXOX_002782"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR7006204.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(562..1164)
                     /gene="ais"
                     /locus_tag="ACHXOX_002783"
     CDS             complement(562..1164)
                     /gene="ais"
                     /locus_tag="ACHXOX_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR7006205.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1472..2611
                     /gene="arnB"
                     /locus_tag="ACHXOX_002784"
     CDS             1472..2611
                     /gene="arnB"
                     /locus_tag="ACHXOX_002784"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR7006206.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2615..3583
                     /gene="arnC"
                     /locus_tag="ACHXOX_002785"
     CDS             2615..3583
                     /gene="arnC"
                     /locus_tag="ACHXOX_002785"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR7006207.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3583..5565
                     /gene="arnA"
                     /locus_tag="ACHXOX_002786"
     CDS             3583..5565
                     /gene="arnA"
                     /locus_tag="ACHXOX_002786"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR7006208.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5562..6452
                     /gene="arnD"
                     /locus_tag="ACHXOX_002787"
     CDS             5562..6452
                     /gene="arnD"
                     /locus_tag="ACHXOX_002787"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR7006209.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6452..8104
                     /gene="arnT"
                     /locus_tag="ACHXOX_002788"
     CDS             6452..8104
                     /gene="arnT"
                     /locus_tag="ACHXOX_002788"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR7006210.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8101..8436
                     /gene="arnE"
                     /locus_tag="ACHXOX_002789"
     CDS             8101..8436
                     /gene="arnE"
                     /locus_tag="ACHXOX_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR7006211.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8436..8822
                     /gene="arnF"
                     /locus_tag="ACHXOX_002790"
     CDS             8436..8822
                     /gene="arnF"
                     /locus_tag="ACHXOX_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR7006212.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(8816..9082)
                     /gene="pmrD"
                     /locus_tag="ACHXOX_002791"
     CDS             complement(8816..9082)
                     /gene="pmrD"
                     /locus_tag="ACHXOX_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR7006213.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(9192..10547)
                     /gene="menE"
                     /locus_tag="ACHXOX_002792"
     CDS             complement(9192..10547)
                     /gene="menE"
                     /locus_tag="ACHXOX_002792"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR7006214.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(10544..11506)
                     /gene="menC"
                     /locus_tag="ACHXOX_002793"
     CDS             complement(10544..11506)
                     /gene="menC"
                     /locus_tag="ACHXOX_002793"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR7006215.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(11506..12363)
                     /gene="menB"
                     /locus_tag="ACHXOX_002794"
     CDS             complement(11506..12363)
                     /gene="menB"
                     /locus_tag="ACHXOX_002794"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR7006216.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12378..13136)
                     /gene="menH"
                     /locus_tag="ACHXOX_002795"
     CDS             complement(12378..13136)
                     /gene="menH"
                     /locus_tag="ACHXOX_002795"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR7006217.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13133..14803)
                     /gene="menD"
                     /locus_tag="ACHXOX_002796"
     CDS             complement(13133..14803)
                     /gene="menD"
                     /locus_tag="ACHXOX_002796"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR7006218.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14892..16187)
                     /gene="menF"
                     /locus_tag="ACHXOX_002797"
     CDS             complement(14892..16187)
                     /gene="menF"
                     /locus_tag="ACHXOX_002797"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR7006219.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16266..16571)
                     /gene="elaB"
                     /locus_tag="ACHXOX_002798"
     CDS             complement(16266..16571)
                     /gene="elaB"
                     /locus_tag="ACHXOX_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR7006220.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16626..17087)
                     /locus_tag="ACHXOX_002799"
     CDS             complement(16626..17087)
                     /locus_tag="ACHXOX_002799"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7006221.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
BASE COUNT         4024 a         4588 c         4343 g         4160 t
ORIGIN      
        1 agtgcagtta tcatcgcaaa agagattact gtcactttca catatttctg agttaccgta
       61 ttctcatggt ttctttttat ttataaggaa tacccctgtg cgacaacgga ctattgtatg
      121 ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa atggccgacg atcgcggcgt
      181 tttccccggt caatgggcgc tttcgggtgg cggcgtggag tctggcgaac gaattgaaga
      241 ggcactacgc cgcgaaattc gcgaagaact gggagaacag ctgcttttga cagaaatcac
      301 gccgtggacc ttcagcgatg atattcgcac caagacgtat gcagatggtc gcaaggaaga
      361 gatttatatg atttacctga tttttgactg cgtttctgcc aaccgagaag tgaaaataaa
      421 cgaagagttt caggactacg cgtgggtaaa acctgaagat ctggtgcatt atgatttaaa
      481 tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt taacaatgat gacagccatc
      541 actggctgtc agattgaaaa tttagtggtt aacgaattcc ccatccagat aaactttgcc
      601 tttttccaca tgcatgacta aaccatccag ataatcaggt ttaaatgtcg catcacgctt
      661 atttttagca atatatgtca ggcaatgatt atgggtgaaa ataacgatat ttttatcagg
      721 cgctttgctt tgtaagtcct taattgcact ataaatctca ttaccgcact gaagaagtcg
      781 tttatctacc gtcagttttt tacccgcgga aaaccaggtc gccgactgaa tggtccggac
      841 ggtattactg gaataaagat cgaaatcagg gatgtcagcg ctaaaagcgt tgcccagttc
      901 acgggcatcc tgggtacctt taaccgtaat acctgtttta tctgacaagc attgattggt
      961 tgaacgatcg caacgttcag catggcgaaa caaaacgaca accgggtgct gctgtgccag
     1021 tctggccaat gttttattgt cgatacgtgg caaaccattg ctactccagg cggcatgagt
     1081 acccagtccg gcaattgcag cgagcgccag taaaatgatg aaatattttt ttgacttcaa
     1141 tgatgagcgg caaaaagcta acacgtaaac tccacctata gacaagcgca accagacaat
     1201 taccgtgaaa ttgagcgaca tttctggcga taattagcag ttggtgtaat attaaaaatc
     1261 ctacgatgtc ggcaaaatgc ctcaaaattt tgccaaatgc aaagcctaaa taagaaaaaa
     1321 tataaaaatt tcaatattta cgtctaatat tagtttctta aggttaagtt aatattctat
     1381 ccttaaaatt tcgctccaaa tggcaaaata tacacaacac tctttatagc aaatataagt
     1441 ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa tttttgcctt tttcgcgacc
     1501 agcaatgggc gtggaggaac tcgctgcagt taaagaggtt ctcgaatccg gttggatcac
     1561 aaccggtccg aagaatcagg cgcttgagca agccttttgc cagttgacgg gaaatcagca
     1621 tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc acgctaatgg cgttggaaat
     1681 tggcaagggc gatgaagtga ttacgccttc cctgacctgg gtttcaaccc tcaatatgat
     1741 ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac cgcgatacgc tgatggtcac
     1801 gcctgaagcg atcgagtcag ccattacgcc acgcactaaa gccatcattc cggtgcatta
     1861 tgccggtgcg ccagcagata ttgacgccat tcgcgccatt ggcgaacgtt acggcatcgc
     1921 agttatcgaa gatgctgccc atgccgtcgg tacgtattac aaagggcgac atattggcgc
     1981 aaaaggtacc gctatttttt catttcatgc cattaaaaat attacctgtg cggaaggtgg
     2041 cctgattgta actgataatg aaaaccttgc ccgccagcta cggatgctga aatttcacgg
     2101 tctgggtgtc gatgcctatg acagacaaac ctggggccgt gcaccgcagg ctgaagtctt
     2161 aacaccgggc tataagtaca atctgaccga tattaacgcc gcgattgccc tgacacagtt
     2221 agcaaaatta gagcacctca acacccgtcg gcgcgaaatt gcccagcaat atcagcaagc
     2281 actggcagct ctcccctttc agccattaag ccttcccgcc tggccgcacg ttcacgcctg
     2341 gcatctgttt attattcgtg tcgatgaaca acgttgtggt atcagtcgcg atgcgttgat
     2401 ggaagcgtta aaagaaagag gcattggtac cgggttacat ttccgcgccg ctcacacaca
     2461 aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg aataccgaat ggaatagcga
     2521 acgcatctgt tctttgccgc tgttcccgga tatgactacc gccgatgccg accgtgtcat
     2581 cacagccctt cagcaactcg caggacaata agccatgttt gaaatccacc ctgttaagaa
     2641 agtctcggtg gttattcccg tttataacga gcaggaaagc ttaccggaat taatcaggcg
     2701 caccaccaca gcctgtgaat cgttggggaa agagtatgag atcctgctga ttgatgacgg
     2761 cagtagcgat aattccgcgc atatgctggt cgaagcctca caagcggaga acagccatat
     2821 tgtgtctatt ttgcttaacc gcaattacgg gcaacattca gcgattatgg cgggattcag
     2881 tcacgttact ggcgacttaa ttattaccct tgatgccgat ctccagaatc cgccagaaga
     2941 aatcccccgc ctggtggcaa aagccgatga aggttacgac gtggtaggga ctgtacgcca
     3001 gaaccgccag gacagctggt ttcgtaaaac agcttcgaag atgattaaca ggcttattca
     3061 gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg ctgcgcgcct atcgccgtca
     3121 tattgtcgat gcgatgttgc actgccatga acgtagcacc tttatcccga ttctggcgaa
     3181 tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat gccgagcgtg agtttggtga
     3241 atccaaatac agttttatgc gcctgattaa tttgatgtac gacctggtga cctgccttac
     3301 cacaacgccg ctacgtatgc tgagtctgct cggcagcatt attgcgattg gaggttttag
     3361 cattgcggtg ctgctggtga ttttacgcct gaccttcgga ccacaatggg cggcagaagg
     3421 cgtctttatg ctatttgccg tgctgtttac ttttattggc gctcagttta tcggcatggg
     3481 attgctcggt gaatatatcg gcaggatcta caccgatgtc cgcgcccgcc cccgctattt
     3541 tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa taatgaaaac cgtcgttttt
     3601 gcctaccacg atatgggatg cctcggtatt gaagctctgc tggctgccgg ttacgaaatt
     3661 agcgccattt ttacccatac cgataatccc gctgaaaaag ccttttatgg ttcggtggct
     3721 cgtctggcgg cggaaagagg cattccagtt tatgcgccgg ataacgttaa tcatccgctg
     3781 tgggtggaac gcattgccca actctcacca gaggtgattt tctcttttta ttatcgccat
     3841 cttatttgcg acgagatttt gcagctcgct cccgcaggtg catttaatct gcacggttcg
     3901 ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg tgctggtgaa cggtgaaacg
     3961 gaaacgggcg ttacattgca ccgaatggta aaacgtgccg atgctggggc cattgtggcg
     4021 caactgcgcg ttgccattgc gccagacgat atcgctatta cgctgcatca taaattgtgc
     4081 catgccgcgc gccagctact ggaacagaca ttacccgcca ttaaacacgg taatattctg
     4141 gaaattgccc agcgcgaaaa cgaagccacc tgttttggtc acagaacgcc agatgacagt
     4201 ttccttgaat ggcataaacc ggcatccgta ctgcacaaca tggtacgtgc cgttgccgat
     4261 ccgtggccgg gtgccttcag ctatgttggc aatcagaaat tcaccgtctg gtcgtcgcgt
     4321 gttcatcctc atgccagcaa agcacagccg gggagcgtga tttctgttgc gccactgctg
     4381 attgcctgtg gcgatggcgc gctggaaatc gtcaccggac aggcgggcga cggcattact
     4441 atgcagggct cgcaattagc gcagatgctg ggcctggtgc aaggttcacg cttgaatagc
     4501 cagcctgcct gcaccgcccg acgccgtacc cgggtactca tcctcggggt gaatggcttt
     4561 attggcaacc atctgacaga acgcctgctg cgcgaagatc attatgaagt ttacggtctg
     4621 gatattggca gcgatgcgat aagccgtttt ctgaatcatc cgcattttca ctttgtcgaa
     4681 ggcgatatca gtattcattc cgaatggatt gagtatcacg tcaaaaaatg tgatgtcgtc
     4741 ttgccgctgg tggcgatagc cacgccgatt gaatataccc gcaacccgct gcgcgtattt
     4801 gaactcgatt ttgaagagaa tctgcgcatt atccgctact gcgttaagta ccgtaagcga
     4861 atcatcttcc cgtcaacttc agaagtttat gggatgtgta gcgataaata cttcgatgag
     4921 gaccattcta atttaatcgt cggcccggtg aataaaccac gctggattta ttcggtgtca
     4981 aaacaattac ttgatcgggt gatctgggcc tatggcgaaa aagagggttt acagttcacc
     5041 ctcttccgcc cgtttaactg gatgggacca cgactggata accttaatgc agcgcgaatt
     5101 ggcagctccc gcgctattac gcaactcatt ctcaatctgg tagaaggttc accaattaag
     5161 ctaattgatg gcggaaaaca aaaacgctgc tttactgata tccgggacgg tatcgaggcg
     5221 ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg gcgaaattat caacattggc
     5281 aatcctgaga acgaagcgag cattgaggaa ctgggcgaga tgctgctggc gagcttcgaa
     5341 aaacatccgc tgcgccatca cttcccaccg tttgcgggct ttcgtgttgt cgaaagtagc
     5401 agctactacg gcaaaggata tcaggacgta gagcatcgta aaccgagcat ccgcaatgcc
     5461 cgccgctgcc tggactggga accgaaaatt gatatgcagg aaaccatcga cgaaacgctg
     5521 gatttcttcc tgcgcaccgt tgatcttacg gataaaccat catgaccaaa gtcggcttac
     5581 gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt gccacgtctg ctggaaatct
     5641 tgagtaagca taatattcag gccagcattt ttttcagcgt cggcccggac aatatgggcc
     5701 gccatctctg gcgactggtg aagccacagt ttttgtggaa gatgctgcgc tcaaacgcgg
     5761 catcgcttta tggctgggat attttactgg caggtacggc ctggccaggt aaagagattg
     5821 gtcatgccaa tgccgatatc attcgtgaag cggctaaaca tcacgaagtc ggcctgcacg
     5881 cctgggatca ccatgcctgg caagcccgta gcggtaactg ggatcggcaa acaatgatcg
     5941 acgatattgc acgcggtctt cgcactctgg aagagattat cggtcaaccg gtaacctgtt
     6001 ctgccgctgc gggctggcgt gccgaccaga aggtgatcga agcaaaagaa gcgttccatt
     6061 tgcgctacaa cagcgattgt cgtggggcca tgccgttccg tccactgctc gaatcaggaa
     6121 accctggcac tgcgcaaatt ccggtgacct tacccacctg ggatgaagtg attggtcggg
     6181 atgtgaaagc agaagatttt aacggttggt tactcaaccg catcctgcga gataaaggca
     6241 cgccggttta taccattcat gcagaagttg aaggctgcgc ttatcagcat aattttgtgg
     6301 atctcctcaa acgcgcagct caggaaggcg tgacattttg ccctttaagc gaactgttat
     6361 cagagacgtt gccgctcgga caagttgttc gcggaaatat tgccggacgt gaaggctggc
     6421 tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg gtacgttacc ttatcggcct
     6481 cttcgcgttt attgcctgct attacctgtt accgatcagc acgcgtctgc tctggcaacc
     6541 cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg gcatccggcg actggattgt
     6601 gccccatctg ttagggctac gttatttcga aaaacccatt gccggatact ggattaacag
     6661 cattgggcaa tggctatttg gcgcgaataa ctttggtgtg cgggcaggcg ttatctttgc
     6721 gaccctgtta actgccgcgc tggtgacctg gtttactctg cgcttatggc gcgataaacg
     6781 tctggctcta ctcgccacag taatttatct ctcattgttt attgtctatg ccatcggcac
     6841 ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg gcgggaatgt gcagcttctg
     6901 gctggcaatg caggcacaga cgtggaaagg caaaagcgca ggatttttac tgctgggaat
     6961 cacctgcggc atgggggtga tgaccaaagg ttttctcgcc cttgccgtgc cggtattaag
     7021 cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat ctctttattt acggctggct
     7081 ggcggttatc agttgcgtac tgacggttct cccctgggga ctggcgatag cgcagcggga
     7141 gcctgacttc tggcactatt ttttctgggt tgagcatatt caacgctttg cactggatga
     7201 tgcccaacat agagctccgt tctggtacta cgtgccggtc atcattgccg gtagcctgcc
     7261 gtggctggga ttactccccg gtgcactgta cacaggctgg aaaaaccgca agcattccgc
     7321 aaccgtctat ttgttgagct ggacgataat gccgctgctg tttttctccg tcgctaaagg
     7381 taaattgccc acctatattc tttcctgctt tgcatctctg gcaatgctga tggcgcatta
     7441 cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg attaatggct ggattaacat
     7501 cgcttttggc ttcactggca ttattgccac atttgtggtc tccccgtggg gaccaatgaa
     7561 cacgccggtg tggcaaacct tcgagagcta taaagtcttt tgtgcctggt cgattttttc
     7621 gctatgggca tttttcggct ggtacacctt aacaaacgtc gaaaagacct ggtcttttgc
     7681 cgcgctttgc ccgctggggc tggcgttgct ggtaggattt tcaattcctg acagagtcat
     7741 ggaaggaaaa catccgcaat tttttgtcga gatgacacaa gaatcacttc agccaagccg
     7801 ctatattctt actgatagcg tcggtgttgc cgcaggtctg gcatggagcc tgcaacgcga
     7861 tgacatcatc atgtatcgcc agacaggtga gttgaaatac ggccttaatt atccggatgc
     7921 gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg cttaatcaac atcgtcagga
     7981 ggggattatt acactcgtgc tttcggttga ccgcgatgaa gatatcaaca gtctcgccat
     8041 tccgcccgca gatgccatcg atcgtcagga gcgtctggtg ctgattcagt atcgtcccaa
     8101 atgatctggc taacattagt ctttgccagc ttgcttagcg ttgccgggca gttgtgtcag
     8161 aaacaggcaa cctgctttgt ggcgataaac aaacggcgca aacatatcgt gctgtggctg
     8221 ggactggcgc tggcttgtct tggtcttgcc atggtgctct ggctgctggt cttgcagaac
     8281 gtaccggtag gcattgccta cccgatgtta agtctgaatt ttgtctgggt gacgctggct
     8341 gcagtaaaac tgtggcacga accggtatcg ccgcgtcact ggtgtggggt ggcgttcatt
     8401 attggcggca ttgtgatcct cgggagtacg gtgtaatggg cctgatgtgg ggattattca
     8461 gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt tgcggcgagt catctgccgc
     8521 cgatgacgca cttatgggat tttatcgcgg ctctgttagc ttttggcctg gatgcccgga
     8581 tactgttact cgggctgctg ggttatctgc tttccgtttt ttgttggtat aaaacgctac
     8641 ataaacttgc gctaagcaaa gcctatgcct tgctcagtat gagttatgtg ctggtatgga
     8701 ttgcttcaat ggtacttccc ggctgggaag ggactttttc gctgaaagca ctactgggag
     8761 tagcctgtat tatgagcggg ttgatgctga ttttcctgcc cacgacaaaa caacgttact
     8821 gagttttccc tgccacttta cactgccgtt cccactcatc ggggctataa ctgcttgccg
     8881 agaggacttt caccgtatgc agcttttcac gattaataca ataaagcgca ttctgcaaag
     8941 gcgagagtaa atcccccact ttcacggcga agtcactctt cacttcggcg atcattttta
     9001 tcgcgccgcc agcatcgcag agcataagta catgtctgtt atcttgttta ttgcagcacg
     9061 attttttgac cagccattcc attgcattat cctgtttgct aagagttttc acatcaattt
     9121 acagattaac agataaacct attatcaacg gcaatggaat ggcaaaattg cgaagtgaaa
     9181 ctaatgattt cttaatcctg gcgttgcacc cactctttta acgcctgacg tgaaatttta
     9241 ataccgccgt ttttcagctc cggcggtaaa gttagccagc gcaccggttg ttgaaaacga
     9301 gccagcttat ctttcaccca ttcgccaaga tcaacggttt gctggtcata ctccaccacc
     9361 gctaccggtc gatgaccaaa ctccttgtcg gcaacgggga cgataaacac ctgcaaaacc
     9421 gcaggatgtg cagcaattac gcgctcgact tcttccggct gaataccctc tccgccactg
     9481 aagaacagat tgtctaaacg tccgacaatg gtcagcttgc cattatgcat ctcaccgcga
     9541 tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa ccagttgccc gttacgccag
     9601 taaccttctg ccatactggc agcccgcagc cacacttcat tattaacgat tttcacttcc
     9661 cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg cttctttcgc acacaccgtg
     9721 gaggcaaact cggtcagacc atagccgcaa aagcaacgaa tcccctgctc gcgcgcctgt
     9781 tccgtcaact cgaccgggat agccgcgccg ccaagtaaca ccgctttcag ggaaacggaa
     9841 ctgcggttaa ccagcaaacg ccagagttgt gttggcacca gtgaagcgtg agtacagcct
     9901 gccagcattt gctccaatgg ctgtttatca cgcaccgtca tccgcgcacc agcgtataac
     9961 cagcgccaca taattccctg accggagacg tgaaacagcg gtaaagagag caaccagtca
    10021 tcgtgatcgc caaacggaat cagcgataac acaccttgcg cactggcaag atgggcctga
    10081 taagtatgga cagcggcttt cggcaaaccg gtagaacccg aggtcaacgt cattgagcac
    10141 agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa cccgctgaat gtgcagcgac
    10201 gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg caaattgcag cgtcagattg
    10261 ggcagcaatt cttcaagcaa cggttgcggc agctgagggt tcacgggcaa cacccgcgcc
    10321 ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg gcgtattcca cgcccgcaac
    10381 atcacgccgc tgccctcaac cacaccctgc gccgcaaagc ccgtagctaa ctcatcgacg
    10441 cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa gacgtaaggc gatggcttct
    10501 ccccgcactt gccgccagtg acgccacggc cagtcagaga agatcataac aactgctcca
    10561 gtacatcagc gtccactaac ggcagtgggc tccccggcca gcgacgaacc tgctgcgcct
    10621 gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg cgttaaccag gcagcaatcc
    10681 gcgccagttg cgttagacct aagctcgact caatggaaga actgatcacc gccgtcagcc
    10741 ccagcgcatg cgccgcctgt acctgctcgc gtactttttc cagactgccc gtgagcgtgg
    10801 gtttgataac taccgcgcgc acgccctctt cagccacaaa ggcaaaatcc ggctcgcgca
    10861 ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc aaacgctcgc gaatcatcgc
    10921 gggttttgca cggctcttcg agaaacgcga tgcggtggcg ataatccggg ttaacatatt
    10981 tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt tgcgtcaaga cgcaaatgca
    11041 gatccggaat tgcctccagc aacagattaa ccaccatgcc gtcgcgcacg gcttcgtaca
    11101 atcccacttt gaccttcgcc actttctcgc ctggcatatc agcaagtttg aggatcagat
    11161 cgtcaggatc gccattacac agtggtgccg cacggtaatt ggccgcctgc gggagcgtat
    11221 ctgccagctc cgccaatgca cagcttacgc caaaggcaac ggaaggcatc tgcggtagct
    11281 cgccatcgcc tgccagccag ttatttaccc aagcaagcag cacaatttgc gcctcttccc
    11341 aggtttcctg actgaagccc ggcagtgggg agatctcccc ccacccttcg cgctcgcctt
    11401 cacgcaggca aacatacagc ccgtcgcggg tttttaaccg cctgtcgcgc agaaccatcc
    11461 ccgcgtccat ggggatctgc cagcggtata cctgcgcgct acgcattacg gattccgttt
    11521 aaatttgctg aaatcaggct gacgtttctg gttgaaagcg ttgcgacctt cctgaccttc
    11581 ttccgtcatg tagaacagca tggtggcgtt gcccgccagc tcctgcagac ccgcctgacc
    11641 gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc gccatcgggc tgttttgcag
    11701 catttcgcgg caccaacgga cggtttcttt ttccagatcc gccagcggta ccacggtgtt
    11761 gacaaggccc atatccagcg cctgttttgc atcgtactga cggcacagga accagatttc
    11821 acgcgctttt ttctgtccga cgatgcgagc catgtaggaa gcgccccagc cgccgtcgaa
    11881 ggaaccgact ttcgggccag tctgaccgaa gatggcatta tctgccgcga tagtcaggtc
    11941 gcacatcatg tgcagaacgt gaccaccgcc gatggagtag ccagccacca tcgcgacaac
    12001 cggtttcgga caggtacgga tctgacgctg gaagtccagc acgttcaggt gatgtacgcc
    12061 ggaatcatct ttatagccgc cgtaatcacc acgcactttc tggtcaccac cggagcagaa
    12121 cgctttatcg cctgcgccag tcagaatgat cacgccgacg ttgtcgtcat aacgcgcatc
    12181 tgccagtgcc tggatcatct cttttaccgt cagaggacgg aaggcattgc gcacctgcgg
    12241 acgattaatg gtgatttttg cgataccgtc ggtggatttt tcataacgaa tgtcctcaaa
    12301 accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc attgcttcat caggataaat
    12361 catattgtgt cctttagtca gaaacgcaag atctgcgcca gacttgcgat aacgccagcg
    12421 ggattttccc gatgcgcgtt atgtccggcg cgaggaatga catggcagtc ggcagccagt
    12481 tccgccgcca aggcgcggaa tttgctgtca cgttcaccac ataaataata aaacgcaaat
    12541 gtgcgggcgc taaggttagc acgtaaatca ggctggacgg cgagagaagt cgcctccagc
    12601 atggcggcaa gggttgcgcc attattgtta ctgcgcagcg ccaccagctc ccggcgttga
    12661 tcgtcattga gcgaggcaaa aacaggctgt tgataccagt cggcaaatac cgccgttaac
    12721 ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac gctgacgttc cgcgcgttgt
    12781 tcagcatttt gcaacccagg atgcccgcct tcgacaacaa ccccacaaag ccccggcatc
    12841 ccctggcaag ccgccatcat cgccacccgt cccccaagcg agtaccccac caaccagaag
    12901 ttaaggatgt tgtaactaac cagggtttta cgcagtaagt cggtgacatc atcaaatcca
    12961 tcgacgctaa tcgccgccga accaccgtga cctgggagat aaacatacaa ccgtgagtag
    13021 tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat cgccggaaaa accgtgcaaa
    13081 aacaccagcc agggtaaacc tggtttgccg tgttttgcct gcgcgtgcag gatcataaat
    13141 ggcttacctg cgccagaagt tgctggagcg tttgcgcacc atcggtgtcg ttaaccacca
    13201 tttcaattac cgtggtggtt ggcgtgcgcc aggcatcggc aagtgccgtt tcaagttcct
    13261 gccagttttg cggacgatga tatttcagct cgaacatcgc ggcggcgtgc tcaaaatgga
    13321 cgttttgcgg catcagatag aaacgctcgc gctcgctttt cggcgttggc agcagcgaga
    13381 aaatttgccc gccgttgttg ttcaccacaa ttaatatcag cggcgcggaa acctgacgca
    13441 ataacgccag cgcgttgaga tcgtaaagtg cggagagatc gcccacaatc gccagcgtcg
    13501 gtttgccgct tgcccgctga acgccggcgg cagtcgagag cagcccgtcg ataccgctgg
    13561 ccccacggtt gctgtacacc gggtaacctg ccggaagttg cgaaagcgca tcaatcagac
    13621 gtaccaccag gctgttaccg acaaacaatt gcccctgttc aggcagatag tcgctgatgc
    13681 gatgcgccag ttgcgcttcg ccaaacgcat cgcgacgggc aataaccgcc tgcattgcct
    13741 gttccgccag gcgcgggatt tcaacgcacc agggctggcg tttttctgcc ggatgcagct
    13801 ccagccagtc ggcaatattg gcaattaagc ggcgtccacg atggtgcgcc ggatcaagcc
    13861 gcccttcgat gtcatccaca atccagtact cttcgggttc acagcttgcc tgccattgca
    13921 ggagccgttt gcccgtcagg ctgcttccca gttgcaccac aatttgcgcc tgttgcagtt
    13981 cgctggtcgc tttggcattg cctaaccaga gatcggcaca cggcagcggc tgtccggttt
    14041 gcgacagcac gtcgccaatc agcggccagc caagagtttg cgcccacagt gcaacttttt
    14101 tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc gcgtttttgt cgccagaaga
    14161 accagtcgcg ctgtttttca ctttccagac gaggcgcttc acgcagccac ggtttgtcgt
    14221 cctgccacca gtcgcccagc cgctgttgcc agctaagccc ggtgtcgtcc atttcgccat
    14281 acagcggttc ggcaaacggg cagttgatat ggactccacc agaatgaagc gtaccgagag
    14341 catggtcgat ggtagaaacc agccaacgtg cggggatatc ctgggtcggg cgcggcaatg
    14401 aaatactgtg cgtggggtga gaggcgaaca ttcccggctg acgaatcgcc tgattcgcgc
    14461 cgcagtcaat cagctccggc gggcgatcgg cggttaacag gatcagtttt tctccggtta
    14521 acccagcttc aatcagtgcc ggatagagat ttgctaccgc cgtaccggag gtcacaatca
    14581 ccgccaccgg ctgcttgctg actttcgcca gccccagcgc cagatgcccc aacccacgct
    14641 catcgaaatg ggtgtggtga atgaatgcgg aattctccgc cgccgctaac gttaacggtg
    14701 tagaacgcga gccaggggcg atacagatgt gtctgacgcc gtgacgcgtt aatgcttcca
    14761 gaatgaccgc cgcccagcgt cggttaaatg cgcttactga catgagtttg tccggtatca
    14821 atattgcggc taagtataag gagctacaaa aatagattat tgatatgaat cggtaatgat
    14881 gcgactcatt actattccat ctgtaataaa gtacgcagcc ctgccgcttt gttgtcgatt
    14941 tcctgccact cttgctcggg gtcggaacca cggacaatgc ccgcgccagc atataatcgc
    15001 acgacattgc cgctaatttt tgctgagcgc agggaaacgc agaattcgct ttgctgtaat
    15061 gagagatagc ccgcagaacc ggcgtaccat tctcgggtga acggttcgtg acgggcgata
    15121 aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg ccgttggctg caactgatgt
    15181 aaacagatca cattatccgc tttgttgagt gaagtccaga tacagcggcg aagatgctgc
    15241 actttacgca gacgcagtac ctgcggcggt aaaacatcca gcgtctgggt atcggcctgt
    15301 aaccgttggc agatatcttc caccaccagc atattctcgc gctggttttt atcatccgcc
    15361 atcagccact cacctaactg ctgcgcctgc ttatcatcag gatgatttgc taccgttccc
    15421 gccagcgctt cagtacgcag cgctttgtca cgccgccgcc ataaccgttc cggtgaagag
    15481 ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat ggtagcaatt cagattcagt
    15541 cgacgactgg cagccatcat cgccgccgcg ttgaccggac ttgcgaaatg caggtcagtt
    15601 gcccgagcga gcaccacttt gtcgagctca ccttcggcga tggttttcgt tgccagttcg
    15661 attaattgcg tccagcccgt tttgtccggc cagtgttgtt ctcgcgtggt ggttaaatgt
    15721 aacccaggca agggcttgat actcaccagt gtggcgataa attctcttgc cttaatcgca
    15781 tcgtgctgaa gggagctttc gctgaatagc gtcagccgca gcgtggcttt accgccacag
    15841 cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg acgggtcaaa cgcattcagc
    15901 ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa gaaaacgttg tgcctggtcc
    15961 aacgacgtaa aacgggtaat cgcgcccagg acggcagctt cttcatcacc attacgttgt
    16021 tgccagtaga attgcgggta tgtttgctga ctggccagcc agctcaaggc atcaaaagcg
    16081 tctttgagag ggaaaggaat atcgataacc cgaatgccgg gtgtcgccgg aatctcttgc
    16141 gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg attgcacgcg gacctctccc
    16201 tgttaaaagc ctcacattat acggggtact acaaaaaaaa tgcagtaccc cggtgtaggg
    16261 aggttttaac ggcgtgccag caacagtcct agtaccagcc caacggccgc gcccacacca
    16321 attccttgcc agggtttttc gtggacgtag tcatcagcac gataaactgc ctgcttcgcc
    16381 cgatagtaat aactgtctga agcctggcta acccgttttt tcacatcatc cagcgctttt
    16441 tctgcacgcg ctttcagctc aacatatttc tgatcggcgg gatcgccaga ggagcggagc
    16501 acctcttcca gtgtttcact aagcagcgtc aggtcgtcat cgatacgtgt atcaccaaac
    16561 tgattagaca ttctcgttct ccatttgcgt aaaacctgtc actaactata gacaagggtt
    16621 gttgattacg cctgaattac ctcgcgtgcc atcccaatgt gtgggatacc atcttcttca
    16681 tagacctccg tcacagggat aaaaccgaaa ctctggtaga agttttgcag atgcgcctgc
    16741 gccccgaggt aaacaggctt atcaggccag tgatgcgtgc aggtgtcgag ggttttactc
    16801 atcagttgct gccccacttt ttcgccacgc aacgcttcac tgacaatcac ccgaccaata
    16861 acgaccggct caagatcatc atcacttttc agaatcctcg catatgccac cagttcatca
    16921 tttttccaac cgagaatatg gcgattatcc cccgtcaaat catcaccatc gatatcctga
    16981 taagggcaat tctgctcgac aacaaacacc gcgcaacgca gttgtaataa ggcataaagc
    17041 tgggaaaccg ataattcaga gtgatgcaga tcttgccatt caatcataat gctctccttt
    17101 tagatgtatg cctta
//
LOCUS       DAZRVY010000082        16574 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZRVY010000082 DAZRVY010000000
VERSION     DAZRVY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.082"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(29..2626)
                     /gene="topA"
                     /locus_tag="ACHXOX_002800"
     CDS             complement(29..2626)
                     /gene="topA"
                     /locus_tag="ACHXOX_002800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR7006222.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3006..3257
                     /locus_tag="ACHXOX_002801"
     CDS             3006..3257
                     /locus_tag="ACHXOX_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR7006223.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3293..4342)
                     /gene="sohB"
                     /locus_tag="ACHXOX_002802"
     CDS             complement(3293..4342)
                     /gene="sohB"
                     /locus_tag="ACHXOX_002802"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR7006224.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4562..5320
                     /locus_tag="ACHXOX_002803"
     CDS             4562..5320
                     /locus_tag="ACHXOX_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR7006225.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5317..5907
                     /gene="cobO"
                     /locus_tag="ACHXOX_002804"
     CDS             5317..5907
                     /gene="cobO"
                     /locus_tag="ACHXOX_002804"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR7006226.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(5947..6819)
                     /gene="rluB"
                     /locus_tag="ACHXOX_002805"
     CDS             complement(5947..6819)
                     /gene="rluB"
                     /locus_tag="ACHXOX_002805"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR7006227.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7032..8146)
                     /locus_tag="ACHXOX_002806"
                     /pseudo
     CDS             complement(7032..8146)
                     /locus_tag="ACHXOX_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(8174..8794)
                     /locus_tag="ACHXOX_002807"
     CDS             complement(8174..8794)
                     /locus_tag="ACHXOX_002807"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR7006228.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(8791..9672)
                     /gene="rnm"
                     /locus_tag="ACHXOX_002808"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(8791..9672)
                     /gene="rnm"
                     /locus_tag="ACHXOX_002808"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR7006229.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9810..9854
                     /gene="trpL"
                     /locus_tag="ACHXOX_002809"
     CDS             9810..9854
                     /gene="trpL"
                     /locus_tag="ACHXOX_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR7006230.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    9831..9924
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            9946..11508
                     /gene="trpE"
                     /locus_tag="ACHXOX_002810"
     CDS             9946..11508
                     /gene="trpE"
                     /locus_tag="ACHXOX_002810"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR7006231.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            11508..13103
                     /gene="trpD"
                     /locus_tag="ACHXOX_002811"
     CDS             11508..13103
                     /gene="trpD"
                     /locus_tag="ACHXOX_002811"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR7006232.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13104..14465
                     /gene="trpCF"
                     /locus_tag="ACHXOX_002812"
     CDS             13104..14465
                     /gene="trpCF"
                     /locus_tag="ACHXOX_002812"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR7006233.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            14477..15670
                     /gene="trpB"
                     /locus_tag="ACHXOX_002813"
     CDS             14477..15670
                     /gene="trpB"
                     /locus_tag="ACHXOX_002813"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR7006234.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            15670..16476
                     /gene="trpA"
                     /locus_tag="ACHXOX_002814"
     CDS             15670..16476
                     /gene="trpA"
                     /locus_tag="ACHXOX_002814"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR7006235.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         3923 a         4442 c         4173 g         4036 t
ORIGIN      
        1 gtttataaaa acctgacaga attaaaggtt atttttttcc ttcaacccat ttgccatcaa
       61 cataaaatgc tgaccagcca gtcgcctttc cgtctttttc cgaagagaca tattgctgtt
      121 tggttttacg gctaaagcga accatggtct tattaccttc cggatcctgc tgtggcgcat
      181 cggccagata acgcagtttt tccggcagac ggtcgcggaa gcgataaagc tcttccacca
      241 gtggcgcacg cgtttcacgc gatttcggga aagtgttggc agccaggaaa acacccgcag
      301 cgccgtcacg cagcacgaaa taagcatctg atttttcgca cggcagctca ggtaatggca
      361 ccggatcttc tttcggtggt gccacttcgc cgttacgtaa aatcttacgt gtgtttttac
      421 actcttcgtt ggtgcaggcc atgtatttac cgaaacgccc cattttcagg tgcatttcag
      481 agccacattt ttcacactca acgatcgggc cgtcataacc tttaatgcgg aattcgccct
      541 cttcgatctc gtaaccgtcg caggttgggt tattaccaca gacatgcaac ttacgtttcg
      601 gatcgatgag atagctgtcc atcgccgtgc cgcatttcgg gcaacgacgt tttgcgcgca
      661 gcgcgttggt ttcagcgtct tcgccttcca gtacgttcag cacttcgttt tccggcacca
      721 ggttaatggt ggttttgcaa cgctctttcg gcggcagcgc ataaccagaa cagccaagga
      781 ataccccggt gctcgctgtg cgaatcccca ttttgcgacc acaagtcggg cagtcaatgc
      841 tggtcagaac catctggttc gggcgcatac caccctcttc cggatctttt tcagctttat
      901 ctaactgctg ggtgaaatcc gagaagaagt gatccagtac agctttccac tctgcttcgt
      961 gatttgccac caggtcgagg ctgttttcca tctgtgcggt gaagtcgtag ttcattaact
     1021 cgcggaaatt ttcttcaagg cgatcggtga cgatttcgcc aattttttcc gcatagaaac
     1081 gacgattttc tactcgcacg tagccacgat cctgaatggt cgaaatgatc gacgcatagg
     1141 tagacggacg accgataccg cgtttttcca gctctttaac cagcgatgct tcactgaaac
     1201 gggctggcgg cttggtaaag tgctgggctg gtgtaagttc aacgagcgtc agagcatcgc
     1261 ctttattaac tgctggtaag atgcgatctt catcgccttt acgcaacgca ggcatcactt
     1321 ttgtccagcc atcaaaacgc aaaatacgac cgcgtgcttt caggcggaaa tcgcccgcac
     1381 caatggtcag cgtcgtggag tcatatttcg ctggggtcat ctggcaggct acgaactgac
     1441 gccagattaa ctggtacagt ttctgcgcat ctgcttccat atccttcagc gattccgcca
     1501 tcacattgac gtcagaagga cgaatcgctt cgtgcgcttc ctgtgagttt tctttgctgg
     1561 cgtactgatt cggactttcc ggcagatatt tcttaccaaa attatcgctg atataaccac
     1621 gaaccatatt taccgcgtcc tgactcaggt tagtggagtc ggtacgcatg taagtgatat
     1681 agcctgcttc atacaaacgc tgcgccatca tcatggtttt tttcacgcca aatccaagac
     1741 gggtgctggc agcttgttgc agcgtagagg taataaaagg agcgccaggt ttactggttg
     1801 tcggtttgtc ttcacgttcc agcacgctgt agcgcgcttt ttccagcaga cttaccgcag
     1861 cctgagtttg ttctttgttg accggacgga acggtttgtc gttctgatga gtcacctgta
     1921 acgccaacgc ttcaccagat ggcgtggtcg tgctggcatc gacttcccag aactcttccg
     1981 gcacgaacgc tttaatttca cgctcacgct cgaccaccag gcgaaccgcc accgactgca
     2041 cacgaccggc agacagacca cgagcgatct ttttccatag cagcggcgaa accatatacc
     2101 ccaccacgcg gtccataaag cgacgcgcct gctgggcatt aacacgatca atattcagct
     2161 cacccggttt gttaaatgcc tggcggatcg cgtttttagt aatttcgtta aacaccactc
     2221 ggctatagcg cgcatcatca cccccaatca cttcccgcag gtgccatgca atggcttccc
     2281 cttcgcggtc aaggtcggtt gcgagataga tgtggtcggc tttttcagcc agttgtttca
     2341 gttcagagac gaccttctct ttaccaggca acacttcata gtgcgcctcc caattgtgcc
     2401 acgggtcaac ccccatacgg ttgacgagag cgccacgttc atccttttta ggctttttag
     2461 ccgtcttggt ggaggtagag tcggcactct ttttggcagc tgagccactg gtcggcaaat
     2521 cgcggatgtg accgacgctg gatttcacca cgtagtcact acccagatac ttgttgatcg
     2581 ttttggcttt tgccggggac tcaacgatga caagagcttt acccatattc accttaccta
     2641 atttgattct tccaggaatg cgtcgcacgt tgataaacct tccactggca acgagttacc
     2701 gatatggact cgttctctgc ggatatcaac cccttgtcgt gactctgttc acgaaaattc
     2761 aggtgactga gttaacaggt cttggcctac aggcgctata gcacgacgga attttggtcg
     2821 aatgtcaagc aaatctattg ccagattgac gaaagcgtca cactgtacct gataaaatcc
     2881 gatacgcaac tttattagca tgcaaaatcc aaagttgcca gtaaggcgct cttgccacca
     2941 gcgtggtata tttgtctgca agccatgcct ggcgtagact aatgtccttg tttaaggagt
     3001 aaataatgaa caaagagaca caacccatcg accgcgaaac gctgttgaaa gaggccaaca
     3061 aaatcattcg tgagcatgag gatacgctcg cagggattga agccaccggc gtcacgcagc
     3121 gtaatggcgt gctggtattt atcggcgact actttttaga tgaacaagga ttgccaacag
     3181 ccaaaagcac cgcggtgttt aatatgttca aacacctggc ccatgtgctt tcagaaaaat
     3241 atcatctggt tgattaaagc aaaacgcgag gtcttagcct cgcgtttgtc ttttacatca
     3301 atggcttttg accccgctgc caccagcgta gcaacaatcg atcggcgctc tctgccgcgc
     3361 tgccggtgaa tcggtcaatg agtcgtttac gctgcatata gcgtacatta accacttcac
     3421 ggccttccat caggctaaga ataacttcat cactggtgtt gatttcatca accaggcctt
     3481 tctctaccgc ctgttgtccg taccagtgtt cacccgttgc cacctgttca atatccagag
     3541 acggacgcat acgcttcaca aaatctttaa ataactgatg cgtttcgttc agctcttcgc
     3601 ggaatttctc ccgcccttct tcggtatttt cacccagcaa ggtcagcgtg cgcttatact
     3661 gcccggcggt gtgcagttcg atatcaatat ctttgctttt caggaagcgg ttaaagttgg
     3721 gcatttgcgc caccacccca atggaaccca caatagcaaa cggtgcggaa acaattttgt
     3781 ccgccacaca ggccatcatg taaccgccgc tggcagcgac tttgtctacc gtaacagtta
     3841 aaggaatgtt tttatcacgc agacgctgca gctgcgaagc cgccaacccg taaccatgca
     3901 ccatgccacc agggctttcc agacggagca caacctgatc ctgcggtttg aatgctgcga
     3961 gtaccgccgt tatctcttca cgtagcgagt tcacttcatg ggcgtccatg ctgcctttaa
     4021 aatccagcac ccagacgcga ggtttactgt cagttgccac ctcgcccagt ttggctttcg
     4081 cttttgctgc tttcgcttct tgcttgtgct ttttcttctg cgctttgtgc cactgttttt
     4141 gctgatgtga gtccatcagc gccgcggcca gttcttcttt catctcctta tactgttcgc
     4201 tgagattgtt gacccgtaac tcgccacgct ggcgtttatt acgttgagca acattgacaa
     4261 taatggcggc aatcgccgca atcgctagca caacggtaac gattttcgcc aaaaacaaac
     4321 catattcaga caacaattcc acgagtcccc accttggtta aacaacgctt tggctcacag
     4381 tttacaacag gcatgttaag ccgtctcgca gagatgttat tgcagcgaaa gtatgacaaa
     4441 gtatccattt tgcggtgtta tcgccccagg ctgattgaaa tgcccttctg tttcaggcat
     4501 aaagccccaa agtcataaag tacactggca gcgcgacaga caacgtcgtt gaggagtagc
     4561 catgcattac cagccaaaac aagatttact caatgatcgc attatcctgg tgacgggagc
     4621 cagcgatggg attggtcgtg aagccgcgat gacgtatgca cgctatggtg cgacagtgat
     4681 tctgttgggc cgtaatgaag aaaaattacg tcaggtagcc agccacataa acgaagaaac
     4741 tgggcgtcag ccacagtggt ttattctcga tttgctgacc tgcacgtccg aaaattgcca
     4801 acaactggca cagcgcattg tcgttaatta tccgcgtctg gatggtgttt tgcataatgc
     4861 cggattgctc ggcgatgttt gcccaatgag cgaacaaaat ccgcaggtct ggcaggacgt
     4921 catgcaggtc aacgttaatg ccacctttat gctcacccag gcactgcttc ctttattact
     4981 caaatcggac gccggttcac tggtctttac ttcatcaagc gttggacgtc agggacgagc
     5041 caactggggt gcatatgcag cgtcgaaatt tgccaccgaa gggatgatgc aggtactggc
     5101 cgatgaatat cagcagcgcc tgcgcgtcaa ctgcattaac ccaggcggta cgcgcaccgc
     5161 aatgcgcgca agcgccttcc cgaccgaaga tccacagaaa cttaaaacac ccgctgatat
     5221 catgccgctc tacctctggc tgatgggcga tgacagccgc cgtaaaaccg gcatgacctt
     5281 tgacgcccaa ccgggccgta aaccaggaat ttcccaatga gtgatgaacg ctaccaacag
     5341 cgtcagcagc gagtgaaaga aaaagtagat gctcgtgtgg cccaggccca ggatgaacgc
     5401 ggtattatca tcgtctttac cggcaatgga aaaggcaaaa ccaccgcggc atttggtacg
     5461 gcaacacgcg cagttggtca cggaaaaaaa gtgggcgtcg tgcagtttat taaaggcacc
     5521 tggcctaatg gcgaacgcaa tctgctggag ccacatggcg ttgagtttca ggtgatggca
     5581 acgggcttta cctgggatac acaaaaccgc gagtctgata ccgccgcctg ccgcgaagtc
     5641 tggcaacatg caaagcggat gcttgctgat tcctcactgg atatggtttt gcttgatgaa
     5701 ctgacgtata tggtggcgta tgactatttg ccactggaag aagtggtgca ggcgttaaat
     5761 gaacgtccac atcaacagac ggtgattatc acgggtcgag gctgtcatcg ggatattctt
     5821 gaactggcgg atacggtaag tgagctgcgc cccatcaaac atgcgtttga tgccggtgtt
     5881 aaggcgcaaa tagggattga ttattaatct gtaaaaatgc cggatatgac gtccggcata
     5941 ctttgattaa ccgttattac gcccgccaga acgacgaccg ccgctcacct gactgtgacg
     6001 tttcaccgca cgacgaatct gattagcctt catgcgacga cggtcttttt ctacagcgac
     6061 tttagagctg gtttctggcg gaagctccac cagttcgcgc agatagttag tctgggcgag
     6121 gtccagctcc gtccagccac cgcgtggcag accttttggc agtgggatat caccgtaacg
     6181 aacacggatc aggcggctca cctgcacacc aaccgcttcc cacagacgac gaacttcacg
     6241 gttacggcct tcggtcagag tcacgttgta ccactggttg atcccttcgc cgccgctgaa
     6301 cttgatggtt ttaaaagctg ccggaccatc ttccagttgc acgccacgac tcaaatcacg
     6361 cagtttcgcg tcgtcaacct gaccaaatac acgcacggcg tattcacgtt caacttcacg
     6421 gcttggatgc attaaacggt ttgccagttc accatcggtg gtgaacagca gcagaccaca
     6481 ggtgttaacg tccagacgac ctacggcaat ccagcgtgcg ccacgcagct ttggcagacg
     6541 gtcaaacacc gtcggacgcc cttccggatc gttacgggta cacaactcac cttccggttt
     6601 gtaataggcc agaacgcgac aaatttgttc agcggactca cgtaccgaaa tcagatgacc
     6661 atcgatacga attttcagcc caggggtaac ttcaacgcga tcgccgagtt tagcaatttt
     6721 gccatcaaca ctcacacgac cggcttcaat aatagattcg atttcacggc gggagccgtg
     6781 gccagcgcgg gccagcactt tctgtaattt ttcgctcata gagcttcctt ttggtgtcgc
     6841 cttcccaggc gtctgatatg cttttgtgca acagtgagtc agaacttaca tctgactcac
     6901 tgatagtaaa gtctgtttga actcttcagg ccttaagtaa acgactcgac gttttaactg
     6961 gcctggcttt gaggcgcaac atactacact atctttatgg ttaaagcttg tttcgattaa
     7021 catcagctta attaccagcc accaccgcct ccgccacccg cgccgccacc agcaccacca
     7081 ccggaataac ttcgccccaa caaactggtt ttggccccca gactcaggtg catctgtaat
     7141 gaaaacaaaa cgccactacg ggcaatttcc ggggcgctaa cagccgctgt gagtgatggg
     7201 gtgatacgtg ctacccattc tcttccaagg tttgctgaga ctgcccaacc tagcaacgcg
     7261 taatccagtt tccgtgtttc ccctttccgc cgacgcccac tgtgtgttgc tgcctcttta
     7321 cgcgcaagat agttaacgat agcttcaccg tgggcataac gttgctgccc agtttgtgta
     7381 aactttggca gaaaaatata ccccatgcca gtgagataac cggtcaggaa aacagcgcta
     7441 aaataacccg ctggcatata ccagtaaaag aatacatctc cgacattgat gtagagagaa
     7501 tagaaggcca cgcctgaaca aatcagtggt aaacaattct tccgtcctca aggatgcggc
     7561 catttcgata tgtgtcgccc tcttctccag taaagagatc gatttcgcta aatggattac
     7621 ccgcgatatc agggaacttg agtgaaaatc gggtcttata gatttcaaac ggccagtggt
     7681 taccagtcac gttccagatt aacagatctg aatctccctc acgcaggaaa gcatttttaa
     7741 cctgataatg aatttgataa tgatagtcgc cttgaggcaa gaaacgttgt ttatcgccca
     7801 cgacaatact cattaacccg atatgccagt catgatgcca gggttcagga ataccatcac
     7861 gggtgacgcc aacaatatga tagttcagca gtgcagcgtc cccatcaggc tgcatccaga
     7921 gtcgtggtaa atcacgataa aatccgtgtt taatctgctg atttcgcgcc tgaaaatgaa
     7981 tattttcttt gatgtcgagc accccatccg gttgcagagt ggcttcaaca tcaaagagcg
     8041 aaatcccttc ttcataagaa gggatctccg tgttttctgc ataacttaga gatgatacaa
     8101 atagccctgc tatcaacagc aaaatgcagc gaaacttccc tgccatagtc ttccatccgt
     8161 ggaacgcaat ttgttataag aaaggcttca catcacctac gccttcacgc accacgaccg
     8221 gcgtatcatc ggtaagatca ataaccgttg tcggtttctg cccgagatag ccgccgtgaa
     8281 taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg gattcggtaa
     8341 attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt gcttcaagca
     8401 acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt ttttcctgca
     8461 acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg cccggcgtgt
     8521 tgtttttcat taaacgaaac gcaacgttat caacaaatga ataggtcgag agttcagaaa
     8581 gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata cgacaaatac
     8641 gctccatcgc gtttttatct tcaattttac agccgagcgc atagccggaa tcagttggat
     8701 aaacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga cgttgctgtg
     8761 ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg tgttctgcgg
     8821 ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt tacgacctag
     8881 ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata aatgatgctg
     8941 acgcgcaagg gccgccagct gggtacgttc attgggcgat tgctggcact gcgcgacttc
     9001 catcgcgtca ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc atttagcact
     9061 aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat gaatgacatc
     9121 aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc cgcgcgccag
     9181 atatttttta aagacatccg ccattgaact ggctttgccg caccccacaa ggaaacgcgc
     9241 aaaatgaccg cgcgtcaccg caccgccctg cgccagtcgt cgcgcgcctt ccagtgcccc
     9301 tggtatttgc gctttttcaa gtctttcggc aatcagttgc gcccgctgat tgcgccgttc
     9361 tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat tcagcccgac
     9421 aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa taagattcag
     9481 cgccagtccc gaacgtgaaa tttcctctct tgctggcacg attgcagctg tgttgtcatg
     9541 gtcggtgatc gccagggtac cgacgcgcat ctcgactgca cggtgcacca atacttctgg
     9601 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcgtaatt
     9661 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacag
     9721 ttctggcaaa tattctgaaa tcagctgttg acaattaatc atcgaactag ttaactagta
     9781 cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg aaagcttggt
     9841 ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag atacccagcc
     9901 cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca aacacaaaaa
     9961 ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac tgcgcttttt
    10021 caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga tatcgacagc
    10081 aaagatgatt taaaaagcct gctactggta gacagtgcgc tgcgcattac agcattaggt
    10141 gacactgtca caattcaggc actttccggc aacggcgaag ccctgctggc actactggat
    10201 aacgccctgc ctgcgggtgt ggaaaatgaa caatcaccaa actgccgtgt gctgcgcttc
    10261 ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc ggtttttgac
    10321 gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga agcaatgttc
    10381 ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc gcaactgtca
    10441 gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat ggtgattgac
    10501 catcagaaaa aaaacacccg cattcaggcc agcctgtttg ctccgaatga agaagaaaaa
    10561 caacgtctca ctgctcgcct gaacgatctt cgccagcagc tgaccgaagc cgcgccgccg
    10621 ctgccggtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga tgaagagttc
    10681 ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt ccaggtggtg
    10741 ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta cgtgctgaaa
    10801 aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac cctgtttggc
    10861 gcgtcgccgg aaagttcgct caagtatgac gccaccagcc gccagattga gatctacccg
    10921 attgccggaa cacgtccacg cggtcgtcgt gccgatggct cgctggacag agacctcgac
    10981 agccgcatcg aactggaaat gcgtaccgat cataaagagc tttctgaaca tctgatgctg
    11041 gtggatctcg cccgtaatga tctggcacgc atttgcaccc ccggcagcag ctacgtcgcc
    11101 gatcttacca aagttgaccg ttactcttac gtgatgcacc tggtctcccg cgtggtcggt
    11161 gagctgcgcc acgatctcga cgccctgcac gcttaccgcg cctgtatgaa tatggggacg
    11221 ttaagcggtg cgccgaaagt acgcgccatg cagttaattg ccgaggcgga aggtcgtcgc
    11281 cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct cgacacctgc
    11341 attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc cggtgctggc
    11401 gtagtccttg attctattcc gcagtcggaa gccgacgaaa cccgtaataa agcccgcgct
    11461 gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg gctgacattc
    11521 tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg cgcagcaatg
    11581 ggcataatgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt gaacgcctgg
    11641 cgacgatgag caatccgata ctgatgcttt ctcctggccc cggtgtgccg agcgaagccg
    11701 gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt ggcatttgcc
    11761 tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg ggcgaaattc
    11821 ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc ggattaacaa
    11881 acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg gccggtttaa
    11941 ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg gatcgcgttt
    12001 gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc ctgctggaac
    12061 aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa ccgattctgg
    12121 aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg ttttcagcgg
    12181 tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc atgaaaattc
    12241 gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa aacgcagcgc
    12301 cgttcccgcg cccagattat ctgtttgctg atatcgtcgg tactggcggt gacggcagca
    12361 acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg ctgaaagtgg
    12421 cgaaacacgg caaccgtagc gtctccagta aatctggttc gtccgatctg ctggcggcgt
    12481 tcggtattaa tcttgatatg aacgccgata aatcgcgcca ggcgctggat gagttaggtg
    12541 tttgtttcct ctttgcgccg aagtatcaca ccggattccg ccacgcgatg ccggttcgcc
    12601 agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac ccggcgcatc
    12661 cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt gccgaaacct
    12721 tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg gatgaagttt
    12781 cattacacgc gccgacaatc gttgccgagc tgcatgacgg cgaaattaag agctatcaat
    12841 tgaccgctga agattttggc ctgactccct accaccagga gcaactggca ggcggaacac
    12901 cggaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac gccgcccatg
    12961 aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat gaagatctgc
    13021 aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac gacagagtta
    13081 ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc gtcgcagaca
    13141 aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt cagaatgagg
    13201 ttcagccgag cacgcgacat ttttatgatg cgctacaggg tgcacgcacg gcgtttattc
    13261 tggagtgcaa gaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc gatccggcac
    13321 gcattgcctc catttataaa cattacgctt cggcaatttc ggtgctgact gatgagaaat
    13381 attttcaggg gagctttgat ttcctcccca tcgtcagcca aatcgccccg cagccgattt
    13441 tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat taccaggccg
    13501 atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag cttgcagccg
    13561 tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag gaactggagc
    13621 gcgccattgc attgggggca aaggtcgttg gcatcaacaa ccgcgatctg cgtgatttgt
    13681 cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac gtgacggtaa
    13741 tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac ttcgctaacg
    13801 gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc gtgcgccggg
    13861 tgttgctggg tgagaataaa gtatgtggtc tgacgcgtgg gcaagatgct aaagcagctt
    13921 atgacgcggg cgcgatttac ggtgggttga ttttggttgc gacatcaccg cgttgcgtca
    13981 acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt ggcgtgttcc
    14041 gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg gcggcagtgc
    14101 aactgcatgg taatgaagat cagctgtata tcgatacgct gcgtgaagct ctgccagcac
    14161 acgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc gagcttcagc
    14221 acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt ttcgactggt
    14281 cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta ggcgcagata
    14341 actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct gctgtagagt
    14401 cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg ctgcgcgcat
    14461 attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt ttggcggcat
    14521 gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt ttgtcagcgc
    14581 gcaaaaagat cctgaatttc aggcccagtt caacgaccta ctgaaaaact atgccgggcg
    14641 tccaaccgca ctgaccaaat gccagaacat tacggctggc acgaacacca cgctgtatct
    14701 gaagcgcgaa gatttgctgc acggcggcgc gcataaaact aaccaggtgc tcggtcaggc
    14761 tttactggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccggtg ccggtcagca
    14821 tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta tttatatggg
    14881 tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa tgggtgcgga
    14941 agtgatcccg gtgcatagcg gttccgcgac gctgaaagat gcctgtaacg aggcgctgcg
    15001 cgactggtcc ggtagttacg aaaccgcgca ctatatgctg ggcaccgcag ctggcccgca
    15061 tccttatccg accattgtgc gtgagtttca gcggatgatt ggcgaagaaa ccaaagcgca
    15121 gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg gcggcggttc
    15181 gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc tgattggtgt
    15241 ggagccaggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa aacatggtcg
    15301 cgtgggtatc tatttcggta tgaaagcgcc gatgatgcaa accgaagacg ggcagattga
    15361 agaatcttac tccatctccg ccggattgga tttcccgtct gtcggcccac aacacgcgta
    15421 tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag cccttgaagc
    15481 cttcaagacg ctgtgcctac acgaagggat catcccggcg ctggaatcct cccacgccct
    15541 ggcccatgcg ctgaaaatga tgcgcgaaaa cccggaaaaa gagcagctac tggtggttaa
    15601 cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga aagcacgagg
    15661 ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg caaagaaggc
    15721 gcattcgttc ctttcgtcac gctcggggat ccgggcattg agcagtcatt gaaaattatc
    15781 gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gcatcccctt ctccgaccca
    15841 ctggcagatg gcccgacgat tcaaaacgcc actctgcgcg cctttgcggc aggtgtgact
    15901 ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac cattcccatt
    15961 ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt ttatgcccag
    16021 tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccaattga agagtccgcg
    16081 cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg cccgccaaat
    16141 gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac ctatttgctg
    16201 tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa tcatctggtt
    16261 gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat ttccaccccg
    16321 gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg ttcagccatt
    16381 gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc actgaaagtt
    16441 tttgtacaac cgatgaaagt ggcgacgcgc agttaatccc ccagccgcca gttccgctgg
    16501 cggcatttta actttcttta acgaagccgg aaaaatccta aattcattta atatttatct
    16561 ttttaccgtt tcgc
//
LOCUS       DAZRVY010000083        16404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZRVY010000083 DAZRVY010000000
VERSION     DAZRVY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.083"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHXOX_002815"
     CDS             <1..189
                     /locus_tag="ACHXOX_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006236.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            412..909
                     /gene="ubiC"
                     /locus_tag="ACHXOX_002816"
     CDS             412..909
                     /gene="ubiC"
                     /locus_tag="ACHXOX_002816"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR7006237.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            922..1794
                     /gene="ubiA"
                     /locus_tag="ACHXOX_002817"
     CDS             922..1794
                     /gene="ubiA"
                     /locus_tag="ACHXOX_002817"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR7006238.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHXOX_002818"
     CDS             complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHXOX_002818"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR7006239.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHXOX_002819"
     CDS             4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHXOX_002819"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR7006240.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5021..5629
                     /gene="lexA"
                     /locus_tag="ACHXOX_002820"
     CDS             5021..5629
                     /gene="lexA"
                     /locus_tag="ACHXOX_002820"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR7006241.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5702..7027
                     /gene="dinF"
                     /locus_tag="ACHXOX_002821"
     CDS             5702..7027
                     /gene="dinF"
                     /locus_tag="ACHXOX_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR7006242.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7143..7352
                     /locus_tag="ACHXOX_002822"
     CDS             7143..7352
                     /locus_tag="ACHXOX_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR7006243.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHXOX_002823"
     CDS             complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHXOX_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR7006244.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8227..8481
                     /gene="yjbL"
                     /locus_tag="ACHXOX_002824"
     CDS             8227..8481
                     /gene="yjbL"
                     /locus_tag="ACHXOX_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR7006245.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8505..9212
                     /locus_tag="ACHXOX_002825"
     CDS             8505..9212
                     /locus_tag="ACHXOX_002825"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR7006246.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9575..10612
                     /gene="dusA"
                     /locus_tag="ACHXOX_002826"
     CDS             9575..10612
                     /gene="dusA"
                     /locus_tag="ACHXOX_002826"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR7006247.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10746..10988
                     /gene="pspG"
                     /locus_tag="ACHXOX_002827"
     CDS             10746..10988
                     /gene="pspG"
                     /locus_tag="ACHXOX_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR7006248.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="ACHXOX_002828"
     CDS             complement(11154..12137)
                     /locus_tag="ACHXOX_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR7006249.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /gene="dnaB"
                     /locus_tag="ACHXOX_002829"
     CDS             12220..13635
                     /gene="dnaB"
                     /locus_tag="ACHXOX_002829"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR7006250.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="ACHXOX_002830"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="ACHXOX_002830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR7006251.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..16213
                     /gene="tyrB"
                     /locus_tag="ACHXOX_002831"
     CDS             15020..16213
                     /gene="tyrB"
                     /locus_tag="ACHXOX_002831"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR7006252.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3904 a         3911 c         4343 g         4246 t
ORIGIN      
        1 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
       61 cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt
      121 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata
      181 ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg
      241 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
      301 atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
      361 aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac
      421 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg
      481 caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga
      541 aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa
      601 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
      661 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg
      721 gagctggcgt tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg
      781 acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc
      841 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
      901 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
      961 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     1021 tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc
     1081 gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag
     1141 tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag
     1201 aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg
     1261 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg
     1321 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     1381 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     1441 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac
     1501 gatgatgtga agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg
     1561 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     1621 ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa
     1681 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     1741 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     1801 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     1861 gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt
     1921 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     1981 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     2041 ccggttcggc gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     2101 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     2161 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     2221 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     2281 gctgtagggg gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     2341 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     2401 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     2461 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     2521 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca
     2581 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     2641 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     2701 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     2761 gctgacgtga tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt
     2821 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     2881 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     2941 aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct
     3001 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     3061 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     3121 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     3181 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     3241 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     3301 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     3361 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     3421 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     3481 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     3541 gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     3601 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     3661 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     3721 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     3781 agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat
     3841 ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     3901 acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc
     3961 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     4021 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt
     4081 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     4141 gcgtgccgtc gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg
     4201 cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     4261 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     4321 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     4381 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     4441 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     4501 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     4561 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     4621 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     4681 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     4741 atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     4801 taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt
     4861 atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     4921 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     4981 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     5041 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     5101 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     5161 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     5221 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     5281 gcttctggcg cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     5341 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     5401 tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     5461 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact
     5521 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     5581 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     5641 gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     5701 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     5761 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     5821 tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     5881 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     5941 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg
     6001 ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg
     6061 tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc
     6121 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     6181 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt
     6241 gatggggctg catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc
     6301 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     6361 cgaaatgtcg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     6421 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc
     6481 gcgactgggg agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac
     6541 cgcctatgcg ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg
     6601 tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat
     6661 cgtagcgtta ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     6721 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     6781 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     6841 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     6901 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     6961 cgggctttct ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc
     7021 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     7081 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     7141 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     7201 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     7261 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     7321 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     7381 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     7441 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     7501 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     7561 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg
     7621 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     7681 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     7741 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     7801 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     7861 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     7921 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     7981 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta
     8041 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     8101 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     8161 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     8221 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     8281 aatcaattcg ataatgaaga tgcaatcaaa gataaattag ataatagagg ttcaatcaaa
     8341 ggtaaattaa ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat
     8401 ataattattg caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca
     8461 cgtaaaaaaa tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt
     8521 atattgacga ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg
     8581 accgggctat ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg
     8641 cgactcccat tgaacaatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg
     8701 ttcagctatt ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag
     8761 caataggttc tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct
     8821 taaaattatt tgcttcgctg ttaatttcac tttcatattc tagtgcctac gcagatttat
     8881 cagaaacagt gtatattaat gaaaataatg agagctacct gaaagctcag tttgaaaaat
     8941 tatctcaacg tgatatgaag aagtacctgg gagagatgaa gcgtctggct gatgggggag
     9001 aaatgaattt tgatggctat ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg
     9061 atcctgatat tttaagcaaa atgcgagatg ctgcaccaca attaattagc ttcgcgaagt
     9121 cgtttgaccc aacctcaaag gaagagatta aaatacttac agacacttct aaattaattt
     9181 atgatttgtt cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag
     9241 gcattatata aaaggaccca atatttattg ggttcttttt tctctatcaa tgctattagc
     9301 agggagatat atcaccagag tttaatgtgt gattttttat ttatcgtcga acctggattg
     9361 tttatcattg gccttaacaa agttaacggc taataagatt atttccatca cttcgtgaga
     9421 gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt
     9481 tatggtatag taccccgcta ttgagcctcc tgaatagtga tgctgaataa cataacccca
     9541 tgatatatcg ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa
     9601 aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag
     9661 cgttgcacca atgctcgact ggacggacag acattgccgc tatttcttgc gtctgctttc
     9721 ccgcaatacg ttgctgtata ccgaaatggt gaccacaggg gcgattattc acggtaaagg
     9781 tgattacctg gcgtacagtg aagaagaaca tccggtagcg ttgcaactcg gcggtagcga
     9841 tccggcggcg ctggcacagt gtgcgaagct ggcagaagcg cgtggatatg atgagatcaa
     9901 cctgaatgtc ggctgcccgt ctgaccgggt gcagaacggc atgtttggtg cgtgtctgat
     9961 gggtaatgcg cagctggttg ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc
    10021 agtgacggtg aaaacgcgta ttggcatcga tgaccaggac agctatgaat ttctctgcga
    10081 tttcatcaac accgtttccg gcaaaggcga gtgtgagatg tttatcatcc acgcacgtaa
    10141 agcctggctt tcggggttaa gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc
    10201 gcgtgtgtat caactgaagc gtgactttcc gcatctgaca atgtcgatta acggtggtat
    10261 caagtcgctg gaagaggcca aagcacacct gcaacatatg gatggcgtga tggtcgggcg
    10321 cgaggcgtat cagaatccgg gtattctggc ggcggtagac cgggagatct ttggttcctc
    10381 ggataccgat gccgatccgg tggcggtagt gcgcgccatg tatccgtaca ttgagcgtga
    10441 actcagccag gggacgtatc tcggccatat tacccggcat atgttgggct tgttccaggg
    10501 tattcctggc gcgcggcagt ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc
    10561 agacattaat gtgctggaac acgcgctcaa actggtggcg gataagcgtt aacttttcac
    10621 caaaaagtag tcaaattcac cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat
    10681 caatagatta tttttggcat gattcttgta atgccagcaa gagatttcat atttgggaga
    10741 gcatcatgct ggaactactt tttgtgattg gcttttttgt catgctgatg gtcaccggcg
    10801 tttcgttgct gggcattatc gccgcgctgg ttgtggcgac ggccattatg ttcctcggcg
    10861 gtatgctggc attgatgatt aagttgctgc cgtggttact actggcgatt gcggtggtgt
    10921 gggttattaa ggcgattaaa gcaccaaaag tgccgaaata tcagcgttat gaccgctggc
    10981 gttactaagg gattgtgcgg atgatcacaa cctaaggttt tatccttaga acaaaatagg
    11041 aattgataat caaatctgtc actattgcgc ctctaacaga ttcatcgtgc tgtaccctac
    11101 atacagccga actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcaacaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gcgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ccacgcgcac tttcttaccg
    11521 ccggtgatct cttttaaccg ctcgaccaga tcctcttcac gatagttaat aacctgccac
    11581 gcgcccgctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccaggg cttttgccca ctggcaggca attaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaggatgc cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctaatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gattatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggtaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctga
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca tcggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cggacgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacgggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
    16021 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
    16081 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
    16141 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
    16201 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
    16261 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
    16321 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattatc tggaattcct
    16381 ttccctattt tttcgtgtgg agta
//
LOCUS       DAZRVY010000084        16400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZRVY010000084 DAZRVY010000000
VERSION     DAZRVY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.084"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHXOX_002832"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHXOX_002832"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR7006253.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHXOX_002833"
     CDS             complement(2380..2820)
                     /locus_tag="ACHXOX_002833"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR7006254.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHXOX_002834"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHXOX_002834"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR7006255.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHXOX_002835"
     CDS             3538..4236
                     /locus_tag="ACHXOX_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR7006256.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHXOX_002836"
     CDS             4465..5673
                     /locus_tag="ACHXOX_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7006257.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHXOX_002837"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHXOX_002837"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR7006258.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHXOX_002838"
     tRNA            7780..7856
                     /locus_tag="ACHXOX_002838"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHXOX_002839"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHXOX_002839"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHXOX_002840"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHXOX_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR7006259.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHXOX_002841"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHXOX_002841"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR7006260.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHXOX_002842"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHXOX_002842"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR7006261.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHXOX_002843"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHXOX_002843"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR7006262.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHXOX_002844"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHXOX_002844"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR7006263.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHXOX_002845"
     CDS             complement(14640..15911)
                     /locus_tag="ACHXOX_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR7006264.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4094 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAZRVY010000085        16271 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZRVY010000085 DAZRVY010000000
VERSION     DAZRVY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.085"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHXOX_002846"
     CDS             complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHXOX_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR7006265.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHXOX_002847"
     CDS             complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHXOX_002847"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR7006266.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2358..2432)
                     /locus_tag="ACHXOX_002848"
                     /pseudo
     CDS             complement(2358..2432)
                     /locus_tag="ACHXOX_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2648..4029)
                     /locus_tag="ACHXOX_002849"
                     /pseudo
     CDS             complement(2648..4029)
                     /locus_tag="ACHXOX_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(4039..4989)
                     /locus_tag="ACHXOX_002850"
     CDS             complement(4039..4989)
                     /locus_tag="ACHXOX_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR7006267.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5132..6550)
                     /locus_tag="ACHXOX_002851"
     CDS             complement(5132..6550)
                     /locus_tag="ACHXOX_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR7006268.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="ACHXOX_002852"
     CDS             complement(6550..8097)
                     /gene="fdrA"
                     /locus_tag="ACHXOX_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR7006269.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8087..8950)
                     /locus_tag="ACHXOX_002853"
     CDS             complement(8087..8950)
                     /locus_tag="ACHXOX_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR7006270.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8990..9595)
                     /locus_tag="ACHXOX_002854"
     CDS             complement(8990..9595)
                     /locus_tag="ACHXOX_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR7006271.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9853..10350
                     /locus_tag="ACHXOX_002855"
     CDS             9853..10350
                     /locus_tag="ACHXOX_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR7006272.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10442..11374
                     /locus_tag="ACHXOX_002856"
     CDS             10442..11374
                     /locus_tag="ACHXOX_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7006273.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11416..12504)
                     /locus_tag="ACHXOX_002857"
     CDS             complement(11416..12504)
                     /locus_tag="ACHXOX_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR7006274.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12646..>16271)
                     /gene="ehaA"
                     /locus_tag="ACHXOX_002858"
     CDS             complement(12646..>16271)
                     /gene="ehaA"
                     /locus_tag="ACHXOX_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR7006275.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3772 a         4392 c         3856 g         4251 t
ORIGIN      
        1 actgctggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg gcgaaaaggc
       61 ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct ttgcgattca
      121 ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga
      181 ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact ctattagata
      241 tccattcatt taatcgtgca tcatctctgt atgtttttac gtccagaaca gagagcaata
      301 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
      361 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
      421 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
      481 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
      541 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
      601 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
      661 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
      721 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
      781 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
      841 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
      901 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg
      961 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
     1021 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag aagtattgtg
     1081 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
     1141 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
     1201 acagcgtatt tattaatttt ttcaatctgt tagtgtgcga ttatcgataa caaaacgata
     1261 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
     1321 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
     1381 aatgccgcca atcatagaat cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
     1441 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
     1501 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
     1561 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
     1621 cagggctttt tccgcttcgc gttttgcctc agaagtggta aatgccacca catgtgcccc
     1681 catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga ccacgcccac
     1741 ttttttaccc ggcccggcct gccagtgacg tagcggcgag tacgtggtga tccctgcaca
     1801 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
     1861 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
     1921 cggcgagtta taggtgccga tcatgtgatc acagtagttt tccagcccgt cctcacactc
     1981 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
     2041 tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
     2101 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
     2161 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
     2221 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
     2281 tcctgattag ctatgtgtat ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca
     2341 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
     2401 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
     2461 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
     2521 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
     2581 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
     2641 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
     2701 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
     2761 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
     2821 cagtggctag aacagcgaac gggagaggtt ctgttcgtta ggacgaatat agagctgcgc
     2881 gtagagattg gctttttcca gcatgtcgcc cagaccataa ggcgaccagt ggtcgataac
     2941 gctgtcagtg ccagtcatca cttttacgcc tttgtcgtgc aactgtttga gcggcatatg
     3001 cagcgtgcca atcggcaccg tcgaggcgat agaaatttgc tgcgccgcca tccgattcgc
     3061 cagcgcatcg acctgttgtt cgttgagcgt agccagcgca aaggcgtggc tgatggtcag
     3121 cttacctttc agttgcggcg ttttctctac cgtttcaacc atataattga tggctgccac
     3181 gcccgacgga gtggtttcgt gcaggtgaat atcgacacct ttgtcgtagt ccagcgcaat
     3241 ctgaaacatg gtgtcgaggg atttttccat cgcgccatcg acactggtcg ggtccagccc
     3301 gccgacgtaa tgcgcacccg cctgcatcgc ttcgcgcatt aagggttccg atttcgacag
     3361 cagcaaaccg tgctgcggga aggcgacgat ttcacactcg aagcccgcct gacgccgcgc
     3421 cagcaccgcc tgcaaatttt gcagattttt taggccggaa accggttcga tattgcagtg
     3481 gctgcgggca atggtggtgc ctttcgactg caatagatcg atcagttttt ccgcccgctc
     3541 ctgcgtatac ggttgtaact ccggcagcat tttctgcttg agtttgatca tgtcctggat
     3601 agtggtgcct gccggacgat tgagcgagcg ccacggcccg ccgtagaagg ttttgtcgag
     3661 atgaatatgc atgtcgcgag tggtgggtag catcaactta ccgccagcgt catagtgcgg
     3721 cagcgtggcg tccggatgct gcttgttctc gcgcagggcg acaattttgc cgtcctgaat
     3781 ctccacggtc tggcgcgccg tgcgggtctg caccgccaca ccgttttcgt agtcaaaacc
     3841 ggtttccagc agcacgttat cgagatagta atgcgggtca gtgattttca tggtgttgtt
     3901 cgcttcgcag tttagggtgc cagctaccgc cgcatgggcg agcggcacat aggtaccaaa
     3961 cagcgcggcg gcggtgacca tcttaccgct ctggctcaga aattcacggc ggctattgct
     4021 ttctttcatc ctacgtcctt acgttttaat aatatgggtg cccgtttcac cgcgcagcgc
     4081 cgcaggcagg cattccggcg tggtgataat cacttctttg ccgccctgct ccaggaacgt
     4141 caggctggcg atgatttttg gcaacatgct gccgggagaa aaatgccctt cctgcatata
     4201 gcgagtcatg gtggcaatat ccacccgatc cagcgcctgc tgctgcggtt taccaaagtg
     4261 gatacagact ttttcgacgt cggtggtgat caccagaatg tcggcgtgaa tttcacgggc
     4321 cagcagcgcg gtagagagat ctttgtcaat aaccgcgtcc acgctttggt aatcccccgc
     4381 ttcagtacgc actaccggaa ttccaccgcc gcccgcgcca attacgacaa aaccttgttg
     4441 gatcagcgcc ttaatggcgg gtgcttcgac aatacgtttc ggttccggcg aggcgaccac
     4501 gcgacgatag ccccgtccgg catcttcaac aaaacaccag tcagggtttg ccttttgtag
     4561 tttgtcacgc tggctttcac tgaagaatgc accaatgggc ttggtgggat gggcaaaacc
     4621 tggatcgttt ttatccactt ccacctgagt taccacggtg acggcttttt tctcgccgtg
     4681 acgcgccagt cggttattca gcgcctgttg gatcagatag ccgatgccgc cctgcgtatc
     4741 tgccacacag ttcgccagcg gcgttaaggg cagcccttcg cgctcgtggg caatctccgc
     4801 acggcgtaaa tccagcccga cctgcggccc gttaccgtgg gtcagcacaa tgtcgtaatc
     4861 ggaagccagc atttccagca ccgtatcggc cacggctttc accgcctccg cctgatgctc
     4921 aatcgactgg ctggcgttat ctttgataat gctgttgcca ccaatggcaa cgaccacaag
     4981 ctctttcatg ttgttatgtc cgctacagag ggtgatgagg ttatgaccaa cgccagactg
     5041 cctgatgcgc tacgcttatc aggcctacaa ttctcgggcc atccgtaggc cggataaggc
     5101 gttcacgccg catccggcat aaacatgtaa gtcagacgcc gtaacgttcg caccagccga
     5161 gaatggcttt ctcgaagcaa gcgaatggtg gatgcacaat gcccgcgcca atcatgccga
     5221 cgcccgcatc tttatgggcg atggcggtgt tgatcaccgg cagaatgccg ctgctaccca
     5281 cgcgggtgat gtcgattgcc gacggcacgc ccataaaacc gagcagcgga atggtgacgt
     5341 tggggttttc accgagagtg atttcacgca tctgacggga gaaatcgata gcctcttcca
     5401 ccgtgccgcc caccagcgcg acgattgcag gcgcggtcgc catagcaaat ccaccaatac
     5461 cgtaggtttc ggtgatggcg ctgtcgccga tatccagccc cgaatcttcc ggcttataac
     5521 cggcaaacat cgggccgatc acctgctgcg ccgggccggt aaaccattgc cccggcaggc
     5581 cgctgacccg caggccgaac tcgacgccgt tacgcgccat ggtggtgacc acggtgctgt
     5641 attcgatgcc gtgcgccgca tccatcgccg ctttacacat cgccatccac gtcgggccgg
     5701 agaagtagtc gctgctggcg acaaactcga acacttcgcg ctgttgctcg acggaatagc
     5761 ctgcctgaat gatccccggc gtcagtgcct gaatcagcag cgtcgtcccg gcgttattgc
     5821 ggttatggca ctcatcgccc atatgcagcg cctgcgccag cattaagcgc agatcgatct
     5881 caccgataat tttcatcgcg tcgcgcagca ttggcccctg cacatcgcgc atccagttga
     5941 ggcggtcgat cacgctctga tcgttggcac ccatacgcaa aatcttcgcc atctgctcgc
     6001 tcatattggt gtaagcgatg ttgccgtagg ttttgttttt cacgatgtgc ataaacatcg
     6061 aggccgaggt aacgcccgcc atcgatccca cacagtcgtg ttcgtggcac ggcgagaagg
     6121 tgatctcccc ggaggccgcc agttcagccg cctcgtcgag atctttcgcc agtccttcga
     6181 acaccagcgc tccggtgacc gcgcctttca tcgcgccgca cattttttcc caggtgatcg
     6241 gcggcccggc gtgaagaatg gttttcggcg tcatgcccgg caccacgtta atcgcctgat
     6301 caaaaccaat cagcaccgga tgcgactgga taatgcgctc cagcgcctgc tggttagcgg
     6361 cggcgatttt gtctgccagc ggcgaatcgg caatgttatc cagcgcctgc accacctgca
     6421 tattgccctg ccccggcggc gtccagtcga gttgagtcac ttctacatgc tgctttttca
     6481 ggtcatcgct aaacatggcg atgccgacgt taataacgtt caatggttgg ctaaacagtg
     6541 actggctcat caggcttcct cccctttgca gataaattca cgcgccagca atccggtatt
     6601 ggtgctgctg ctcgccagaa tcactccggc atcaagcagc atctggcttt gttgttctaa
     6661 cgatggcgta tcaagatcgg tacccagcac ataggcgaga atgatcaact cgcgtccttc
     6721 ggcagcggcg atcgctttcg cttctttgat cgcctcgatg gtggagccga ccggatcttc
     6781 atgcgatcca aatccgagca caaaatccat cacgatcacc gccacttctg gatcgcgcgc
     6841 ctcttcgatc aagcgactga tgcggttggt ggggtcaatc attggatgcg gcttgccgtt
     6901 cgtgaagtcg tcatcgccaa agtcgaggaa ggtgtgtttg atgctgcggt tgatatcttg
     6961 caggcggaat tccggatcgg gctgaatgtt gctgtagaca tcgccatgtt tttccatcac
     7021 cgcgaacatg gtttcgtcgc acagcgtgcc gccgcagaac aggccacgaa tgtatttctg
     7081 ctgcggttgc agacgcgcac gcacatcagc aatcaacggc tggttaagcg tatgcaggtc
     7141 gagattttcc tgtttcacgc cggagagcat caccgctttt agcgccgcct ctttgctgcc
     7201 gcgggcaaac tgtagcccct gctcatccac cggcgtttca acgcgaccga ggaagcagac
     7261 caccaccggc ttgcggcagg cacgtgcgcg ttccagcact ttgcgggcca ccgcaggcgc
     7321 gggaggtttt gaaatcagtg cgataatttc agtttgcgga tcgttttcca gcatcccgat
     7381 ggcgtcgagc atcatcaggc cgccgatttt ctcgctcagg tcgcgcccgc cggtgccaat
     7441 cagttgtgaa acaccgccac caaattcatg aatgcggacg ctcagctcct ggctgccggt
     7501 gccggatgcg ccaacaatac cgatattgcc gcgacgcacg gcgttaccaa aacagagcgc
     7561 cgcgccgtta ataatcgccg tgccgcagtc cggccccatc atcagcagcc ctttttcgtg
     7621 ggccaactgc ttgagcgcca gttcatcttc aactgagacg ttatcggaaa acagcatcac
     7681 gttgagatcg ttttgcagcg cctgacgcgc ttcgcgagcg gcaaacagac cgttgaccga
     7741 aatcaccgcc aggttacttt ccgggatatg ctttttggcg ctggcaatag tggcgtaacg
     7801 cgcctcgtgc gagccgcttt gcgctttggt gttgaacagt tcttcaatct ccaccagcaa
     7861 ctgctcgttg tccgcacccg atttaccatt gatgacaatc atcaggtcgc cgtttttcgc
     7921 ctgttccagc gtcggcgtca gcagtcctaa atttttcagc acacctttgt tcatttcggt
     7981 cgccatcgcc acaaatgcct gctcgacgcc gtcgagttta tttgcacgcg tggagataga
     8041 catcagcgag acagaatcaa aataggtatt cggtttaatg actattttaa ctgacataat
     8101 tccctccgta ggtttggctc agcgcacaac catcggccat gccatacagc atgtcgcagc
     8161 cggaactgga gccaatatgt ttaatttttt ctattgcctg agttgaattc ccagggacgc
     8221 cataaataat gttgtgaata aagcgatgaa tattttcccg gcagcgttgt tgtaatgcgg
     8281 cttccagcgt tatggcactt aataacgtgg tgttatttct gccgcgtagc agaccgcgat
     8341 aaaattcctc tttgtatttt tctgccggat gcccgggaat aaataaaata agcgccagac
     8401 ctgttaaata atcatcggct gatggcgtaa ggccaatccc taacccaatc atactggcga
     8461 ccgctgcggc gatattttgc ttatcgttta ctgtctgaat aactgcccgt cgtttaatat
     8521 gtaattcttt atttaacgcc tgataaaacg gattatcgcc gcgataatta aacagggttt
     8581 cattttcccc caggtgctga tgaataatgt cgtgccactg tagccaggcg ataaggtgaa
     8641 aattcacagc ggtcatttgc aaggttgggg acagccaacg ccgacaacga ctcatctcta
     8701 tatgaagatg ttgaccaacc gtaatacctt gatcgtcaaa ctgaacctta tcaccatgcc
     8761 ggaacagatc atcaaagtga gtgagtgcca accgacaact gtttggcgca ttgtcgtact
     8821 cttcacaaag cagcgtcagc aactgctggc gagccgggag tgcgatattt accgctctgg
     8881 caaagacctg ttcaacctgt ccttgccccc gctgcgccag aaaatccgca tccgcagtta
     8941 gcgcccacac acaccgtttt tctggtgtgt tgatagccag cctcccggtt tatgcacctg
     9001 cggcaatcag taactgcgca atctcttcaa agccccgttc ccgcgccagt tccagtggcg
     9061 ttttgccata tttatcggtc agatgcgggc tggcaccgtg ttccagcaat aactgcacaa
     9121 tcgcctgctg tttaatacca ccatcattaa gcacaatcgc ttccagtagc ggcgtccagc
     9181 ctacatggtt ggtctggtta acgttaatct ccgtatgcgc caaaagctct tttacaatac
     9241 ttaaatggcc tttttcacag gcaggcgtca ggccgacacc gccaaaacgg gttacgcaat
     9301 taagatcggg tttagccggt aaaataattc gtagtagcgt taaatcatcg ttcagacagc
     9361 taattaaaaa aggatttaaa caggtatgat cttgcttatt aatatccgct ccggcatcaa
     9421 ttaatgcctg aacgcaagca tattgctgat ataaacttgc cagcgtaatt gccgttttcc
     9481 cctgacgatc gcaggtatta atatcgacac ccagcgcaag gcaggttttt actttatcga
     9541 tatcaccctg ttgtgcagcc aataaatagt cggcaggtag attttttata ctcattcaca
     9601 ttcccctggt ttttgtggct gttttcgatg aattgagaat agcaacgata atctgataaa
     9661 agaacccgct taaatttaat tcgccgtatc tcgccttttt gttgaataat attcaacaat
     9721 cttttcgcag tgtgcggtag tgcaatattt gtttctttga cccgataccc tttcactcaa
     9781 tcaggtttta tttctctata gtctgcacat gtaagagtag ctttcgtcca tgtctaacca
     9841 gaggggaaca agatgaacgg actcacggca acgggtgtca ccgtcggtat ttgtgcaggg
     9901 ctttggcagt tagtgtcttc acatgtcggg ctctctcaag gatgggaatt attaggaacc
     9961 attggcttcg tcgccttttg cagtttttat gccgcaggcg gcggtaaatc ggggtttatc
    10021 agaagtctgg cggttaatta ttccgggatg gtgtgggcgt ttttcgccgc gctgactgct
    10081 ggttggctgg cctctgtaag tggactgtcc gcgttctggg cgagcgtaat tactaccgta
    10141 cccttctcgg cggtagtggt ctggcaaggg cgtttctggt tactgtcctt tattcccggt
    10201 ggctttctcg gcatgacgct gttttttgcc agcgggatga actggacggt gacgttactg
    10261 ggttttctgg cgggtaactg cgtggggata atttcagaat atggtggaca aaaattaagc
    10321 gaagcgacga ctaaacgcga cgggtattaa aagttattca aaaatgatta tctatcttta
    10381 accgatacct ctacagtcat agctggagaa attcatagta gataatggcg agaatggttt
    10441 aatgaactca atttttaccg aagagaatct gttggcattt actaccgctg cgcgttttgg
    10501 cagcttcagt aaagccgcag aagagttggg tttaaccact tccgccatta gctacaccat
    10561 caagcgtatg gagacggggc tggatgtggt gctgttcact cgcagtaccc gcagcattga
    10621 gttaacggag tccgggcgtt attttttccg caaagctacc gacctgctga atgattttta
    10681 tgccatcaaa cgcagtatcg atactatttc gcagggcata gaagcgcgtg tgcgcatttg
    10741 tattaatcag ctgctttata cgcccaaaca caccgcacgc ttgttacagg tgctgaaaaa
    10801 gcagtttcct acctgccaga ttaccgtcac caccgaagtg tataacggcg tctgggatgc
    10861 gattattaat aatcaggcca atatcgccat cggcgcaccc gatacactac tggacggcgg
    10921 cggtattgat tacactgaaa ttggcgcgat tcgctgggct tttgctatcg ccccggatca
    10981 cccgttagcc tttgtgccgg aacccatcgc cgaaagccaa ctgcgtctct accctaatat
    11041 tatggtggag gacaccgcgc atacgattaa taaaaaagtg ggctggttgc tgcacgggca
    11101 ggagtcaatt ctggtgccag atttcaacac caaatgtcag tgccagattt taggtgaagg
    11161 aattggtttt ttgccggatt acatggtccg tgaggcgatg acgcaatccc tgctggttac
    11221 ccgacaaatc cataatccgc gccaggattc gcgcatgtta ctggcgactc agcattctgc
    11281 tacgggtcag gtgacgcagt ggattaaaaa acaatttgcg ccaaatggca tattaacggg
    11341 gatctatcag gatttactgc atcgggagaa ttaatcgctg ccaacctccc gatgcgctgt
    11401 gaatagcggt ttacatcaac cacctgcttt cattacccat tcagagataa atttattacc
    11461 cggtactggc ggagagtaca aatagccctg caaaaagtga acgcctttac cgatcattaa
    11521 atccgcctgc tcctgggttt ctaccccttc cgccacgata ctcagaccag gcttacgcgc
    11581 tagttcgaca atattgtcca caatatgacc ggagatttcg tcgacactcg ccatttgcac
    11641 aaatgactta tcgatcttaa taaaatcgac cgggaacgcc tgcaagtaac gataggtcgc
    11701 ataacccgta ccaaagtcat ccagcgcaaa ggtaatgttg tgctgatgaa ggctgtcaaa
    11761 tatcgctctg gcttctggcg ttaccggaat agggttacgt tccgttagct cgagaaccag
    11821 cttgatttta tcgttaccta atttcttaac caggttcaga cactcttttt caaatcccgc
    11881 tgccaaaaaa caacccgccg agacgttgat gccaatatgg aaattgtccg gcagcaaatg
    11941 ttttaccggc atcagaatat ccgcagtctg tttcatcagt tggcgggtca ttatgacaat
    12001 aagaccggat gactccgcca gaggaataaa ctgatccggt gggataattc ccgtttgtgg
    12061 atgttcccag cggacaagta cctcacagcc cgtcagtacc ccagtctgcg cgcagaaaac
    12121 cggttggatc cacggtttga attcgtggtt ttccaacgcc agactgatgg cttcaggcgt
    12181 gacgatatgg tgacaacggt tatcagggat ataaaaatta tttttatttc ccgtgccact
    12241 gatgacttgc ggatgatatt gaaaaaagat atcccgccag aaggtaatat cactctggat
    12301 gcctagtttt tcatacagct gcttcttttg atgtgaaatt gtctttgcac tgcgatttct
    12361 gtactgtgag atttctgtaa ccgacattcc ctccattagc agcttgagta aaagccgttc
    12421 tttttcggac acgataccag gcaacgtcaa attcaccgtt gccccgaact cttccaaaaa
    12481 aacactcaaa tcatgcgaag ccattgaaca caccttgatt aaatcttgtg cgccagctaa
    12541 atctgcgagt cttcactctg actgataaag ctggctgcaa gaaaatgaca cagagagccg
    12601 aattatctcc cgttcggctc tctctccttt tactcattat tcagatcaga attgccactt
    12661 aatgccaacc attgcagagg tgtcattata tcccctatcg gcaacctgaa cgcccacatt
    12721 cccccagaca ttcaggttgg catttagctg tccttccacc ccggttttta tctcagcaat
    12781 atttcgggct ccatcctgag tgacggacac gccatccata ctggtgctga aatccttact
    12841 gttatgcagc cagttcactt ctacaaacgg ctggaactcg cgggatttac cgtcatccat
    12901 tttatggtgg ctcttcagcc aggtttttac gccaagtcgg gtttgaacat tgccatcacc
    12961 gttgctatga acgagggttc cgttgctttc gcggtgctta tcggctttga ctcccatcca
    13021 ggtaacctgt gcttgcggct gaacatacca ttcattacgc gttccctggc tgccattaaa
    13081 ttcagctaat ttgtgtttgt atccagcttc cagtgaagcg gtaaatcctt ttgatttata
    13141 ggattcactt tgtaagtcat cccctttcac tgtgttatca aaccagctgt actgcgccca
    13201 actgtcgaga tacgcgccat tacgcgactc gtcatcggca taccaggtgg catagaggcc
    13261 ggtgctatat ccgttcacac tcgcttttgc acgataaccg gttcgcgagg aaatggtttt
    13321 gctgtcgctg ttgccatatc ccgccatgac cccaacatgc cagctgtcgc tgccgttttg
    13381 gctccactgc gcgacatcgc ctcccagttg cagaacatag cgattgcttt gggttttcag
    13441 ctggccgctg ccatcacgcc atttattatg accaccttca tggcgcatcc acatagtggt
    13501 ttgtttctgc tcacccgtca ccatgtcggt atagtacgta ttacccagac gctcatgcag
    13561 acgcgtggtg aacattgtat tcgctgctgc aaggttcgca atgtagctac ccgcctccgg
    13621 tcgcaggtca ttatccacat tcagatccgg acccggcgta ggtgtcgggt tagggttcgg
    13681 ctctggattc gggtttggct ccggattcgg catcgggtct ggctccggct gcagttcagg
    13741 agaatcactg ccgctggtca gataccagtt accactattt gccccttgtc cacgcgcgag
    13801 agtgtagtca tacgcccccg caacgatacg accagcctga acaaattcgc cctcagactt
    13861 accgtctaca tggataagtt cgataccgtt aagggttttc gcacctgtac cgccagcgtt
    13921 attcaccgta acggcagtcg tgccgctagt atcgccctcg acaaccaatt tatcggttac
    13981 tgagtcatcg ccattcagta ccgtattcat tactagttga ccgttattac cgtggtagtt
    14041 ccccttcacc actaacacat tgccagcttg ctgtcctgac attccggtca tcagcgtacc
    14101 gttgttggtc agattgccgc ccagggtgta attcacagac gcagccgctc ttttttcagc
    14161 gctattattc aacgccaggg taccgttgtt ctcaacgttt gcgctaccca atccctgcgc
    14221 atgacttacc aggacattag ccgaattagc aatgttcaca ttcgctgtca ggttgctgtt
    14281 atcgcccgcg atgtccagcg tgccgccctg aatattcagc tctccgctcc ccgttaacga
    14341 accggttgaa gtgcccccgt tggtaaccgt cagactgccg ccatttaagc tcagcagtga
    14401 atcggcgctg ccattgagcg tgcccacggt ctggctgtga ccattcatat ccagttcagt
    14461 ctctgccgcc agacgcagtt catgggtatt acccaacacg ttatcgttat tcaacaaaag
    14521 cgtcccactg cgcagatcgg taacaccggt atagtcgttg tctttgttag aaagcgatac
    14581 ggtctgaccc ttctggctgc tgaatgccag gtcaccactg ccggtaatct ttgcgctgag
    14641 atcggcggca ttctcgcttt taccgttaga gctcagcacc agagcatcgc tgtcggtagc
    14701 gtgtaaatca agctgggtca ggccgtaacc gatatacaga ccgtcattgt ttaccccact
    14761 cgtcagacga tagtcgtaag tgccgacggc cgctttttgc ccattctgct gaatatcacg
    14821 ttgggcactg ctggagagga tttgcccatt tttatcctgc aattgcagat tgcccgcatc
    14881 gcccagaact tccgcacctt gcgcatcaac cagcttaatg gcgctgttac catcatcgac
    14941 ttcagtgagc gataacgcag agtcaatatc acgagatact gagcggacaa cgtcactgtc
    15001 agaaacctga atcacaccct cgccgcgcaa atccagcgtt ttactaacgt tgaccgtccc
    15061 ttctgtcatc tgtgcacctg ctgtcagggc accaaaatca acggttccgc cggagaatcc
    15121 caaagtgcta atggagtgag cgcccttacc cactgttacc aggcttccct gacccgcagt
    15181 gatcccgcct ctctgcagaa actcagcatt cttttccagg ttaaatgtcg tgttctggaa
    15241 cgccagtgaa ccctggaacc agtctgtata agcaaactca tcgaaggaga attcgtgatt
    15301 ccccatgttt atttcaacaa tacctctacc ttgaagatcg ttatcaaata cccaatcttt
    15361 attggcattg atttgcagtt tgccatcaac atccaggtcc gcacggccga tgttcttgtc
    15421 ttcagaaata accgcgacag caccagaagc gacatcaata tatccgctga atgcatagtt
    15481 attgccggtc agcgccactg tcccactgtc gatacttatt gtaccgttgc cggaaatctt
    15541 attggcaaag gtcatgctat cgtcagcctt caggtgtaat ttcccgcggt tgttgatttc
    15601 tgcggtacct atattttggg tgatccctaa agcgccattt tgctcaattt tgactttacc
    15661 gctaaaggcg gagttatccc cggttaagta agtcgtacca ttgagcgcat tgatatagcc
    15721 caggccctcg ctgtcactga caatatccgc ggaaatagtc gcgtttttat ccgtgtggtt
    15781 taaggtgatt ttcttctcgg atgtctttgc ccacacaaac tcaatttttt cggcatcaat
    15841 aatccctgca gccttcgctg cgcttccttc ctgtgcacct aacgctaact cagagttggt
    15901 gcttaaacta atagtcggcg cagagatttt accaccttcc gaaacagcaa gtgttgtatc
    15961 ggagttttga tcataataac ccaggctaat tttctcactg gcattaactt caccgccgtt
    16021 attaactgac aggtactgat taccaccgta actgccgtaa acgcccataa agaaggtttt
    16081 agaatttacc tggctgcctt caccactgac atttccctgc aaataggtca gacggctggt
    16141 gcccttaaca tggattttgc cgccgttgga gacattaaaa tagccgcgat cgtgtctcat
    16201 gcctgagttt gactcataca aatcatcagc atataactcg ctgccagcat tggagacatt
    16261 caggaacccc g
//
LOCUS       DAZRVY010000086        16263 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZRVY010000086 DAZRVY010000000
VERSION     DAZRVY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.086"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(94..1296)
                     /gene="ubiI"
                     /locus_tag="ACHXOX_002859"
                     /gene_synonym="visC"
     CDS             complement(94..1296)
                     /gene="ubiI"
                     /locus_tag="ACHXOX_002859"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR7006276.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1319..2497)
                     /gene="ubiH"
                     /locus_tag="ACHXOX_002860"
                     /gene_synonym="visB"
     CDS             complement(1319..2497)
                     /gene="ubiH"
                     /locus_tag="ACHXOX_002860"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR7006277.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2494..3819)
                     /gene="pepP"
                     /locus_tag="ACHXOX_002861"
     CDS             complement(2494..3819)
                     /gene="pepP"
                     /locus_tag="ACHXOX_002861"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR7006278.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3845..4423)
                     /gene="ygfB"
                     /locus_tag="ACHXOX_002862"
     CDS             complement(3845..4423)
                     /gene="ygfB"
                     /locus_tag="ACHXOX_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR7006279.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4591..4920
                     /gene="zapA"
                     /locus_tag="ACHXOX_002863"
     CDS             4591..4920
                     /gene="zapA"
                     /locus_tag="ACHXOX_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR7006280.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4962..5145
                     /gene="ssrS"
                     /locus_tag="ACHXOX_002864"
     ncRNA           4962..5145
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHXOX_002864"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5166..5768
                     /locus_tag="ACHXOX_002865"
     CDS             5166..5768
                     /locus_tag="ACHXOX_002865"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR7006281.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5869..5928)
                     /locus_tag="ACHXOX_002866"
     CDS             complement(5869..5928)
                     /locus_tag="ACHXOX_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7006282.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6260..6319)
                     /gene="ibsC"
                     /locus_tag="ACHXOX_002867"
     CDS             complement(6260..6319)
                     /gene="ibsC"
                     /locus_tag="ACHXOX_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR7006283.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6548..7780)
                     /gene="serA"
                     /locus_tag="ACHXOX_002868"
     CDS             complement(6548..7780)
                     /gene="serA"
                     /locus_tag="ACHXOX_002868"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR7006284.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8036..8695)
                     /gene="rpiA"
                     /locus_tag="ACHXOX_002869"
     CDS             complement(8036..8695)
                     /gene="rpiA"
                     /locus_tag="ACHXOX_002869"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR7006285.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9125..10018
                     /gene="argP"
                     /locus_tag="ACHXOX_002870"
     CDS             9125..10018
                     /gene="argP"
                     /locus_tag="ACHXOX_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR7006286.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10221..12365
                     /gene="scpA"
                     /locus_tag="ACHXOX_002871"
     CDS             10221..12365
                     /gene="scpA"
                     /locus_tag="ACHXOX_002871"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR7006287.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12358..13353
                     /gene="meaB"
                     /locus_tag="ACHXOX_002872"
     CDS             12358..13353
                     /gene="meaB"
                     /locus_tag="ACHXOX_002872"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR7006288.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13364..14149
                     /gene="scpB"
                     /locus_tag="ACHXOX_002873"
     CDS             13364..14149
                     /gene="scpB"
                     /locus_tag="ACHXOX_002873"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR7006289.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14173..15651
                     /gene="scpC"
                     /locus_tag="ACHXOX_002874"
     CDS             14173..15651
                     /gene="scpC"
                     /locus_tag="ACHXOX_002874"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR7006290.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15648..>16263)
                     /locus_tag="ACHXOX_002875"
     CDS             complement(15648..>16263)
                     /locus_tag="ACHXOX_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7006291.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3949 a         4382 c         4139 g         3793 t
ORIGIN      
        1 aattagttta tttcaaatga ggaaaatctc ccggcgaaaa aaccgggaga tgaaagtgtg
       61 atgggtatca aataaacagc agaagagaaa tttttaacgc agccattcag gcaaatcgtt
      121 taatcccatt gcctggcgga taagttgtgg tttaacgcca ggaagcgtgt cggccagttt
      181 caaaccaata tcacgcagca gttttttcgc cggattggta ccggaaaaca gatcgcggaa
      241 tccctgcata ccagccagca tcagcgccgc actgtgcttg cggctacgct cataccggcg
      301 caggtaaatg tactgcccga tgtctttccc ctgacgatgc aaccgtttca gttcagcaat
      361 cagctctgca gcatccataa agccaagatt tactccctgc cccgccagcg ggtgaatggt
      421 atgcgcggcg tcgcccacca gcaccagacg gtgcgcggca aactggcgcg cataacgccc
      481 cgtcagtggg aacacctgac gcgcgctctc aaccttgcat aagcccaggc gattatcaaa
      541 agcgatattt aacgcgcgat taaattcatc ttcacttgcc tgctgcatcc gctgcgcttc
      601 ctctggcgac agtgaccaga caattgagca aagatgcgga tcgctaagcg gtaaaaaggc
      661 cagaatgcct tcgccatgga aaacctgccg cgccaccgca tcatgcggtt cttccgtgcg
      721 aatggtcgct accagcgcgt gatgctgata atcccagaaa gtcagcggaa tatctgcttt
      781 gttgcgcaac caggaattag caccgtccgc gccaatcacc agacgcgccg ttaacatgct
      841 gccatctttc agcgtcagga aagtttcatt ttctccccag gcgacctgct gtaattctgc
      901 gggggccaac agagtgatat ctgacgactg ctgcgctttg ttccacagcg cgtagtgaat
      961 cactgagttt tcaacgatat gcccaagatg gctatagccc atgctttgat cgtcaaacga
     1021 aatgtgacca aaactgtctt tgtcccacac ttccataccg tgataacaac tggccctacg
     1081 agagagaatg tcctgccaga cgccaagacg ggtgagtaat ttttcgctgg cggcattgat
     1141 agccgaaacg cgcagttgtg gtggtgcatc cgccgccaga ggttccggta cgcgctgctc
     1201 cagtacggca acgcgtaagc cgctcccctg taagccgcag gcaaccgcca gccccaccat
     1261 gccgccacca acaatggcta catcaacact ttgcattgtt tattccttaa aaccgccttc
     1321 aacgcgccac ccaaccgagg gtgcgctgcg ccagcacatc gcgtgccggg gtgaataatt
     1381 ccatcgtcat cagtccgatg ttgcgcccga caaccagcgg tgcccaacgg ttggcaaaaa
     1441 gatgtaccag gctgtccgtg acgccgatgg ttgcttcgcg atcgctctgt cgacgctgct
     1501 gatagcggca caatatgccg taatcaccga tgtcttctcc gcgctcatgc gcctgagtca
     1561 gggtttccgc aaggctcatg acatctcgca taccgaggtt aaacccttgc ccggcaatcg
     1621 ggtgcagagt ttgcgccgca ttgcccacca gcacggtacg atgggtaata gctctggcgg
     1681 cgcgggttaa cgccagcgga taagcactgc gtttaccggc gtgggtaatt tttccaagtc
     1741 gccagccaaa ggccgactgg agttcacggc aaaacttctc gtcgctccac gacagcacct
     1801 cttcgcgccg ttccagtgga tgacaccaga ccagcgaaca gcgtccgtca gacatcggca
     1861 acatcgccag cgggccatgt tgcgtgaagc gttcaaaagc gcggccttcg tgcgcaacgg
     1921 aagtcgcaac gttggcaatc acggccagtt gttcgtaagg ctcctgctgc cagtcaacgc
     1981 cgcacgcggt ggctaacgct gaatgggtgc catcagctgc taccagcacg cggcccgtca
     2041 gcgtctcgcc actctccagc gtcacttcaa cgtgactctg agtacgggca acgttagcca
     2101 cgcgatcagg gcaatgcagc gttacgccag gtgctttacg cagcaatgca aacagccgtt
     2161 gcccgacatt gtgcaattcg acaacctgtc ccagcgccgc cagttggtaa tcttctgcgg
     2221 cgagggtgac aaatccagcg tgaccacgat cgctgacatg cacggtagtg atggcggttg
     2281 cgtaatccgc cagagattgc cagacgccga tgcgcgccag ttgctgacag gtccccgcag
     2341 ccagcgcaat agctcgtcca tcaaagcccg gatgagcatg tgactctggc gcggtcgctt
     2401 caatcaaatg taccggcagc gccccgtgac ttaaccggga aatagccagc gccagcgtcg
     2461 cgcccgccat gccgccaccg acgatgatta cgctcattgc tttctcgcag ctgccatcaa
     2521 cgcttcgatt tcttccggct ttttcaccac gctggcggtg aggttttcgt tgccggtttc
     2581 ggtaatcaca atgtcgtctt caatacgaat gccgataccg cgatattgtt ctggcacatc
     2641 cgcatccggc gcgatataca gaccaggctc tacggtcagt accatccccg gttccagaat
     2701 gcgcgagcga tcctgaccat aaacgccaac gtcatggaca tccagtccta accagtggct
     2761 aaggccatgc ataaagaaag gacgatgggc gttctgagcg atcagttcat caacatcacc
     2821 tttcaggatg ccgagtttta ccaggccgct aaccatgatg cgcaccactt caccagtgac
     2881 ttccagaatg gaagttcccg gacgatacag gcgcaggctg gtttcgagag actccagcac
     2941 aatgtcgtag atttcacgct gggcctgggt gaatttgccg ttgaccggga aggtgcgggt
     3001 aatatcgccc gcgtaacctt tgtattcaca acccgcgtca atcaacacca ggtcaccgtc
     3061 gcgcagttca ctctcgtttt cggtgtagtg cagaatgcag ccgttttcac cgctgccgac
     3121 aatggtgtta taggacggat agcgcgcacc gtggcggtta aattcgtggt gaatttcgcc
     3181 ttccagatgg tactcgaaca ttcccggacg gcatttttcc atcgcccgtg tatgtgccat
     3241 ggcggtgatt tctcccgcgc ggcggagtac ggcaatctct tctggcgatt tgaacaggcg
     3301 catttcatga acaacaggac gccagtcgat catcgttgcc ggtgcggtga gattttgccg
     3361 cgaaccttta cgcagttttt ccagcgcact gttcatgatt tcatcagcat atgcatattc
     3421 gccctgggca tggtaaacca catccaggcc gttaagtagt tgataaagtt gctgattgat
     3481 ttcgctgaat gccagtgcgc ggtcaacgcc cagtttctct ggcgcggcat cctggcctaa
     3541 gcgacggcca aaccagatct ccgccgtcag gtcgcgaacg cggttaaaca gaacgctgtg
     3601 gttatgagtg tcatcgcttt taatcagcac cagcaccgct tccgattcgt taaagccggt
     3661 gaagtaccag aagtcactgt tctgacgata ggggtattcg ctgtcggcgc tacgtgttac
     3721 ttctggtgca gcaaaaatca gcgcggcgct gccgggttgc atttgctcca ccagggcctg
     3781 acggcgacgc tgaaactctt gccgggatat ctcactcata acactctcct tacgtttttt
     3841 gtttttagtg tagagtcggt ttttgtactt ctggcgcggt cggttgcgga tgagtaaagg
     3901 tgtcgtggca taacagcgcg gcaacacgaa cgtattcgat gatctcttca agcgacattt
     3961 caagctcttc ctgatcttcg tcttcgtcgt aacccagttg cgcaatgtta cgcagatcgt
     4021 cgatagcttc accggtttcg ccggtcactt tatccagctt cggttgcgta acgccaagac
     4081 caagcaggaa gtgattgacc caacctgcca atgcatcagc ccgatcgaaa acgctgacat
     4141 catcgccatc aggcagataa agctgaaaaa ggaagccgtc atcctgcagg gcatcgctgg
     4201 tggcagagtg cattttacgc agtgcctgtg ccagctcatg accgaaagcc atgccttcgt
     4261 tcgtcaggtc gtgaagtagc ggcagccatg agctgtcatc gttaccgcca catatcatcc
     4321 cactgattaa accatgcatc tcggcagggg tcagccccgt cccttgttgg ttcagatact
     4381 ggttcatttc gttgtaacca ggcatttcgt tctgtataga cataagcatt cgtcgtcaaa
     4441 gggaggaata ttcatgatat gctaccactt tgggccctgg tggaccagaa aagggcttgt
     4501 ctcttctcat cagggtagct atagtgtcgc cccttcgcag accatgggtc taaagacgaa
     4561 ggcagcgcag tcaatcagca ggaaggtggc atgtctgcac aacccgtcga tatccaaatt
     4621 tttggccgtt cactgcgtgt gaactgcccg cctgaccaaa gggatgcgtt gaatcaggca
     4681 gcggacgatc tgaaccaacg gttgcaagat ctgaaagaac gcactagagt cacaaatact
     4741 gaacagttgg tcttcattgc cgcattgaat atcagctatg agttagcgca agaaaaagcg
     4801 aagactcgtg actacgcggc aagtatggaa cagcgtattc ggatgctgca gcagaccata
     4861 gaacaagcgt tacttgaaca aggtcgcatc accgaaaaaa ctaaccaaaa ctttgaatga
     4921 cacttttcgg tttactgtgg tagagtaacc gtgaagacaa aatttctctg agatgttcgc
     4981 aagcgggcca gtcccctgag ccgatatttc ataccacaag aatgtggcgc tccgcggttg
     5041 gtgagcatgc tcggtccgtc cgagaagcct taaaactgcg acgacacatt caccttgaac
     5101 caagggttca agggttacag cctgcggcgg catctcggag attcccttct tatctggcac
     5161 cagccatgac gcaactacca gaactcccac tgacattatc ccgacaagaa atccgcaaaa
     5221 tgattcggca acgtcgtcgt gcgttaacgc cggaacaaca gcaggaaatg ggtcaacaag
     5281 ccgctacccg gatgatgact tatcccccgg tggtgatggc gcatacggtc gctgtattcc
     5341 tctcttttga tggtgaactc gacacccagc cgctaataga acaactctgg cgcgccggta
     5401 agcgcgtata tcttccagtt ttgcatccct ttagtgccgg taatttgctg ttcctgaatt
     5461 accatccgca aagcgaactg gtgatgaaca ggttgaagat ccatgagcca aaactggatg
     5521 tgcgtgacgt gctacccctt tcccgattag acgtgctgat cacaccgctg gtcgcctttg
     5581 atgagtacgg tcagcgcctg ggaatgggcg gtggttttta tgatcggacc ttacaaaact
     5641 ggcagcacta taaaacgcaa ccggtgggtt atgcgcatga ttgtcagttg gtggaaaaac
     5701 tccccgttga agagtgggat atccctcttc ctgcggtggt tacaccgtcg aaagtctggg
     5761 agtggtaagg gcgatacacc tgcatcgccc tgattgacat cgttgattct ttaacctaat
     5821 ttagtgagta agggtaaggg aggattgctc ctcccctgtg actgactgtt aataagctgg
     5881 gaaacttatg agtaacagta caatcagtat gatggcaagt cgcatcataa cccttctcct
     5941 tcaagccctc gcttcggtga gggctttacc gttacagccc catgctgccc agctatcgta
     6001 agctctcttt aaataaccac aacgcattga tctaccttta aatcttttct tcgagcgtgc
     6061 tcgaaaaacc catgcaaaac agagataatt tttgggaaaa aggatctatt acggcagtaa
     6121 acgtgggcga tgaagtggct atcaaaaagt tcggttcagg gcgatgcacc ccgcatcgcc
     6181 ctgattgaca tcgttgattc tttgacctaa tttagtgagt aagggtaaga gaggatgact
     6241 cctcccctga gactgactgt taataagctc cgaaacttat cagtaacagt acaatcagta
     6301 tgatgacaag tcgcatcata acccttctcc tttaagccct cgcttcggtg agggctttac
     6361 cgttacagcc ccatgctgtc ccgccatcgt aaatccccat taaataaaca caacgcattg
     6421 atctgagttt gatttatttt ctggagcaga ctcgcaaagt agaatgcgca acgcggcaac
     6481 ggtgtggaga agggataaaa aaacgggcaa gtcagtgacc tgcccgttaa ttttcagaga
     6541 aggggaatta gtacagcaga cgggcgcgaa tggtacccgg aatagctttc attgcctgca
     6601 gcgctttttc ggcaacgtct tcgtcagctt caatatcaat aactacataa cccatctggg
     6661 cggaagtttg tagatattgc gcggcgatgt tgacgccctg ctcggcgaag attttgttca
     6721 gcgcagttag cacgcccgga cggttttcgt ggatgtgcat cagacgacga ccaccatgca
     6781 gtggcagcga gacttccggg aagttcaccg cagagagcgt tgagccattg tcagaatact
     6841 tgatcaattt acccgcaact tccaggccga tattctcctg cgcttcctga gtcgaaccgc
     6901 caatgtgtgg cgtcagaagg acgttgtcga attcacacag tggagaggta aatggatcgc
     6961 tattggtcgc cggttccgtc gggaatacgt cgattgccgc ccccgccaga tgtttgctcg
     7021 ccagcgcatc acacagcgcc ggaatatcca ccacagtacc gcgcgaggca ttaatcagca
     7081 gcgagccggg cttcattagc gaaatctctt tcgcgcccat catatttttg gtggacggat
     7141 tctctggtac atgcagactc accacatcgc tcatattcag caggtcagaa agatgctgta
     7201 cctgagtggc gttgcccagc ggcagtttgt tttcaatatc ataaaagtaa acatacattc
     7261 ccagcgattc agccagaatg cccaattgcg taccaatatg accgtagccg atgataccca
     7321 gctttttgcc gcgcgcttca aaagaacccg ccgccagttt gttccacacg ccacggtgcg
     7381 ctttagcatt ggcttccggc acgccgcgca atagcagcag cagttcgcca atcaccagct
     7441 ccgcaacaga gcgcgtattt gagaacggtg cgttaaatac cgggatcccg cgctttgccg
     7501 ccgcatccag atcaacctgg tttgttccga tacagaaaca gccaatagcg accagttttt
     7561 ctgcggcgtt gatcacgtct tcagtcagat gggtacggga tcgcaggccg atgaagtggg
     7621 catcgcggat ggattctttt aattgttcat catccagcgc gcctttgtga aattcgatgt
     7681 tggtgtaacc agctgcacga aggctttcca gcgccttttg gtgcacgcct tctaccagca
     7741 gaaacttaat cttgtctttc tccagcgata cctttgccat ttacccaatc ctgtcttttg
     7801 aaatgttgtg tgcggatttg catccgcctt tcaacatatc aaaaaataat attgcggcaa
     7861 tatgaacgtt tgcgtcgcga tgttgaggaa atatcacgca aaggtatttc ttggttgaat
     7921 atgctggttt agaggattta tttagagcaa tcgacaatag ccaagtaaaa acgtgacaca
     7981 tgtcaccaaa tttaatgaag agaatttttt taacggggga ggttcccccg tcagatcatt
     8041 tcacaatggt tttgacaccg tcaggtgtgc caatcagcgc aacgtccgcg ccacggttag
     8101 caaacaagcc aacagtcacc acgccaggaa tcgcatttat ggcgttttcc atcgctatcg
     8161 ggtcaaggat ttccatgccg tggacgtcga ggatcacgtt gccgttatcg gtcaccacgc
     8221 cctgacggta ttccggacga ccgcccagtt tcaccagctg gcgcgccact gcactacgtg
     8281 ccatcgggat aacttctact ggcagcggga atttgcccag aatatcaacc tgcttggaag
     8341 cgtcagcaat acagataaat ttttccgcaa ccgaagcaat gattttttca cgggtcagcg
     8401 ccgcgccgcc gcctttgatc atttgcatgt ggccgttgat ttcatctgcg ccatcaacgt
     8461 agatgccaag gctgtcgact tcgttgagat caaaaacgtg aatgccgagg cttttcagtt
     8521 tttcagtgga agcatctgaa ctggaaacgg ccccttcaat ctggcctttc attgtaccga
     8581 gcgcgtcaat aaagtgtgcg gcggtggaac ctgtacctac accaacaatg gtgccgggct
     8641 gaacatactg aagtgccgcc catcctactg cttttttcaa ttcatcctgc gtcatgatcg
     8701 tttcgcctgt ggtatgaaat ttcacacgca ttatatacaa aaaaagcgat tcagaccccg
     8761 ttggcaagcc gcgtggttaa ctcatccata aaatatcgcg caatggcggg catccccttt
     8821 cgccccgcaa ataaagcata caacggtctg ggtatgccgc tccacggtgc gaacaggcgc
     8881 accagttccc cccgttcgca agcccctgtt tacaggcaaa ttgaggcaat aacgccaccc
     8941 cattcaacac aaccagggca cgaactcgct gagcgaaatg cattagcgca aatccctgat
     9001 cttttaagct gcgcgtttaa ccacggatac aaatgttacc cgccgacggc ttcggtatat
     9061 gcaacctgac acaaaattgt gtcatagtgc aggaaaaagc atttaccagg agcagacaac
     9121 agcaatgaaa cgcccggact acagaacatt acaggcactg gatgcggtga tacgtgaacg
     9181 aggatttgag cgcgcggcac aaaagctgtg cattacacaa tcagccgtct cacagcgcat
     9241 taagcaactg gaaaatatgt tcgggcagcc gctgttggtg cgtaccgtac cgccgcgccc
     9301 gacggaacaa gggcaaaaac tgctggcact gctgcgccag gtggagttgc tggaagaaga
     9361 gtggctgggc gatgaacaaa ccggttcgac tccgctgctg ctttcactgg cggtcaacgc
     9421 cgacagtctg gcgacgtggt tgcttccggc actggctcct gtgttggctg attcgcctat
     9481 ccgcctcaac ttgcaggtag aagatgaaac ccgtactcag gaacgtctgc gccgcggcga
     9541 agtggtcggc gcggtgagta ttcaacatca ggcgctgccg agttgtcttg tcgataaact
     9601 tggtgcgctc gactatctgt tcgtcagctc aaaacccttt gccgaaaaat atttccctaa
     9661 cggcgtaacg cgttcggcat tactgaaagc acctgtggtc gcctttgacc atcttgacga
     9721 tatgcaccag gcctttttgc agcaaaactt cgatctgcct ccaggcagcg tgccctgcca
     9781 tatcgttaat tcttcagaag cgttcgtgca acttgctcgc cagggcacca cctgctgtat
     9841 gatcccgcac ctgcaaatcg agaaagaact agccagcggt gaactgattg acttaacgcc
     9901 agggctattt caacgccgga tgctctactg gcaccgcttt gctcctgaaa gccgcatgat
     9961 gcgtaaagtc actgatgcgt tgctcgatta tggtcacaaa gtccttcgtc aggattaatc
    10021 catcaaataa tgcctgatag cacatatcag gcgttatcct cacttctttt tgtattcctt
    10081 gaatcacatc acaaaataga caaatctcag gcggcaaaaa acgacgtctg aatgcatttt
    10141 tttgctggcg acaaacccac gtaaaaagct caccgtaagc gcaaataccc tcattttgat
    10201 tgcgttttac ggagcaaata atgtctaacg agcaggagtg gcaacagctt gccaacaagg
    10261 aattgagccg tcgggagaaa actgtcgact cgctggttca gcaaaccgcg gaagggatcg
    10321 ccatcaagca gctgtatacc gaagccgatc tcgataatct ggaggtgaca ggtacccttc
    10381 ctggtttgcc gccctacgtt cgtggcccgc gtgccactat gtataccgcc caaccgtgga
    10441 ccatccgtca gtatgctggt ttttcaacag caaaagagtc caacgctttt tatcgccgta
    10501 acctggccgc cgggcaaaaa ggtctttccg ttgcgtttga ccttgccacc caccgtggct
    10561 acgactccga taacccacgc gtggcgggtg acgtcggcaa agcgggcgtc gctatcgaca
    10621 ccgtggaaga tatgaaagtc ctgttcgacc agatcccgct ggataaaatg tcggtttcga
    10681 tgaccatgaa tggcgcagtg ctaccagtac tggcgtttta tatcgtcgcc gcagaagagc
    10741 aaggtgttac acctgataaa ctgaccggca ccattcaaaa cgatattctc aaagagtacc
    10801 tctgccgcaa cacctatatt tacccaccaa aaccgtcaat gcgcattatc gccgacatca
    10861 tcgcctggtg ttccggcaac atgccgcgat ttaataccat cagtatcagc ggttaccaca
    10921 tgggggaagc gggtgccaac tgcgtgcagc aggtagcatt tacgctcgcc gatgggattg
    10981 agtacatcaa agcagcaatc tctgccggac tgaaaattga tgacttcgct cctcgcctgt
    11041 cgttcttctt cggtatcggc atggatctgt ttatgaacgt cgccatgttg cgtgcggcac
    11101 gttatttatg gagcgaagcg gtcagtggat ttggcgcaca ggatccgaaa tcactggcgc
    11161 tgcgtaccca ctgccagacc tcaggctgga gcctgactga acaggatccg tataacaacg
    11221 ttatccgcac caccattgaa gcactggctg cgacgctggg cggtactcag tcactgcata
    11281 ccaacgcctt tgacgaagcg cttggtttgc ctaccgattt ctcagcacgc attgcccgca
    11341 acacccagat catcatccag gaagaatcag aactctgccg caccgtcgat ccactggccg
    11401 gatcctatta cattgagtcg ctgaccgatc aaatcgtcaa acaagccaga gctattatcc
    11461 aacagatcga cgaagccggt ggcatggcga aagcgatcga agcaggtctg ccaaaacgaa
    11521 tgatcgaaga ggcctcagcg cgcgaacagt cgctgatcga ccaaggcaag cgtgtcatcg
    11581 ttggtgtcaa caagtacaaa ctggatcacg aagacgaaac cgatgtactt gagatcgaca
    11641 acgtgatggt gcgtaacgag caaattgctt cgctggaacg cattcgcgcc acccgtgatg
    11701 atgccgccgt aaccgccgcg ttgaacgccc tgactcacgc cgcacagcat aacgaaaacc
    11761 tgctggctgc cgctgttaat gccgctcgcg ttcgcgccac cctgggtgaa atttccgatg
    11821 cgctggaagt cgctttcgac cgttatctgg tgccaagcca gtgtgttacc ggcgtgattg
    11881 cgcaaagcta tcatcagtct gagaaatcgg cctccgagtt cgatgccatt gttgcgcaaa
    11941 cggagcagtt ccttgccgac aatggtcgtc gcccgcgcat tctgatcgct aagatgggcc
    12001 aggatggaca cgatcgcggc gcgaaagtga tcgccagcgc ctattccgat ctcggtttcg
    12061 acgtagattt aagcccgatg ttctctacac ctgaagagat cgcccgcctg gccgtagaaa
    12121 acgacgttca cgtagtgggc gcatcctcac tggctgccga tcataaaacg ctgatcccgg
    12181 aactggtcga agcgctgaaa aaatggggac gcgaagatat ctgcgtggtc gcgggtggcg
    12241 tcattccgcc gcaggattac gccttcctgc aagagcgcgg cgtggcggcg atttatggtc
    12301 caggtacacc tatgctcgac agtgtgcgcg acgtactgaa tctgataagc cagcatcatg
    12361 attaatgaag ccacgctggc agaaagtatt cgccgcttac gtcagggtga gcgtgccacg
    12421 ctcgcccagg ccatgacgct ggtggaaagc cgtcacccac gtcatcaggc gctaagtaca
    12481 cagctgcttg acgccattat gccgttctgt ggtaacgccc tgcgactggg cgttaccggc
    12541 acacccggcg cggggaaaag tacctttctt gaagcctttg gcatgttgtt gattcgtgag
    12601 ggattaaagg ttgctgttat tgcggtcgat cccagcagcc cggtcactgg cggtagcatt
    12661 ctcggggata aaacccgcat gaatgacctg gcgcgtgccg aggcggcgtt tattcgcccg
    12721 gtaccctcct ccggtcatct gggcggtgcc agtcagcgag cacgggaatt aatgctgtta
    12781 tgcgaagcgg cgggttatga cgtagtgatt gtcgaaacgg ttggcgtcgg gcagtcggaa
    12841 acggaagtcg cccgcatggt ggactgtttt atctcgttgc aaattgccgg tggcggcgat
    12901 gatctgcagg gcattaaaaa agggctgatg gaagtggctg atctgctcgt tatcaacaaa
    12961 gacgatggcg ataaccatac caatgtcgcc attgcccggc atatgtacga gagtgccctg
    13021 catattctgc gacgtaaata cgacgaatgg cagccacggg ttctgacttg tagcgcactg
    13081 gaaaaacgtg gaatcgatga gatctggcac gccatcatcg acttcaaaac cgcgctaact
    13141 gccagtggtc gtttacaaca agtgcggcaa caacaatcgg tggaatggct gcgtaagcag
    13201 accgaagaag aagtactgaa tcacctgttc gcgaatgaag atttcgatcg ctattaccgc
    13261 cagacgcttt tagcggtcaa aaacaatacg ctctcaccgc gcaccggcct gcggcagctc
    13321 agtgaattta tccagacgca atattttgat taaaggaatt tttatgtctt atcagtatgt
    13381 gaacgttgcc actatcaaca aagtggcggt cattgagttt aactatggac gaaaacttaa
    13441 tgccttaagt aaagtcttta ttgatgatct tatgcaggcg ttaagcgatc tcaaccggcc
    13501 ggaaattcgc tgtatcattt tgcgggcgcc aagtggatcc aaagtcttct ccgcaggtca
    13561 cgatattcac gaactgccgt ctggcggtcg cgatccgctc tcctatgatg atccattgcg
    13621 tcaaatcacc cgcatgatcc aaaaattccc gaaaccgatc atttcgatgg tggaaggtag
    13681 tgtttggggt ggcgcatttg aaatgatcat gagttccgat ctgatcatcg ccgccagtac
    13741 ctcaaccttc tcaatgatgc ctgtaaacct cggcgtcccg tataacctgg tcggcattca
    13801 caacctgacc cgcgacgcgg gcttccacat tgtcaaagag ctgattttta ccgcttcgcc
    13861 aatcaccgcc cagcgcgcgt tggctgtcgg catcctcaac catgttgtgg aagtggaaga
    13921 actggaagat ttcaccttac aaatggcgca ccacatctct gagaaagcgc cgttagccat
    13981 tgctgttatc aaagaagagc tgcgtgtact gggcgaagcg cacaccatga actccgatga
    14041 atttgaacgt attcagggga tgcgccgcgc ggtttatgac agcgaagatt accaggaagg
    14101 gatgaacgct ttcctcgaaa aacgtaaacc taatttcgtt ggtcattaat ccctgcgaac
    14161 gaaggagtaa aaatggaaac tcagtggaca aggatgaccg ccgatgaagc ggcagaaatt
    14221 atccagcata acgacatggt ggcatttagc ggctttaccc cagcgggttc gccgaaagcc
    14281 ttaccgaccg cgattgcccg cagagctaac gaacagcatg aggccaaaaa gccgtatcaa
    14341 attcgcctgc tgacgggggc gtcaatcagc gccgccgctg acgatgtact ttctgacgcc
    14401 gatgctgttt cctggcgtgc gccatatcaa acatcgtccg gtttacgtaa aaagatcaat
    14461 cagggcgcgg tgagtttcgt tgacctgcat ttgagcgaag tggcgcaaat ggtcaattac
    14521 ggtttcttcg gcgacattga tgttgccgtc atcgaagcat cggcactggc accggatggt
    14581 cgagtctggt taaccagcgg gatcggtaat gcgccgacat ggctgctgcg ggcgaagaaa
    14641 gtgatcattg aacttaatca ctatcacgat ccgcgcgttg cagaactggc ggatattgtg
    14701 attcctggcg caccaccgcg gcgcaatagc gtgtcgatct tccatgcaat ggatcgcgtc
    14761 ggtacccgct atgtgcaaat cgatccgaaa aagattgtcg ccgtcgtgga aaccaacttg
    14821 cccgacgccg gtaatatgct ggataagcaa aatcccatgt gccagcagat tgccgataac
    14881 gtggtcacgt tcttattgca ggaaatggcg catgggcgta ttccgccgga atttctgccg
    14941 ctgcaaagtg gcgtgggcaa tatcaataat gcggtaatgg cgcgtctggg ggaaaacccg
    15001 gaaattcctc cgttcatgat gtattcggaa gtgctacagg aatcggtggt gcatttactg
    15061 gaaaccggca aaatcagcgg ggccagcgcc tccagcctga caatctcggc cgattccctg
    15121 cgcaagattt acgacaatat ggattacttt gccagccgca ttgtgttgcg tccgcaggag
    15181 atttccaata acccggaaat catccgtcgt ctgggcgtca tcgctctgaa cgtcggcctg
    15241 gagtttgata tttacgggca tgcaaattca acgcacgtag ccggggtcga tctgatgaac
    15301 ggcatcggcg gcagcggtga ttttgaacgc aacgcgtatc tgtcgatctt tatggctccg
    15361 tcgattgcta aagaaggcaa gatctcaacc gtcgtgccaa tgtgcagcca tgttgatcac
    15421 agcgaacaca gcgtcaaagt gatcatcacc gaacaaggga tcgccgatct acgcggtctt
    15481 tccccgcttc aacgcgcccg cactatcatt gataattgtg cacatcctat gtatcgggat
    15541 tatctgcatc gctatctgga aaatgcgccc ggcggacata ttcaccacga tcttagccac
    15601 gtcttcgact tacaccgtaa tttaattgca accggctcga tgctgggtta atggtttgtc
    15661 ccgtcacgat cttcagcaat atgctgaagc atcgtggcgg tataacgatg atttctgatt
    15721 gaataaagat attcctgcat atacatcggc ctgtgcggcg catcaaaata tttcagcgtt
    15781 gccggattca ccagtcgcca ggcaaaatgc gggatcaccg tcagaaaatg tccgcgctcc
    15841 acggcgctga ttttggccat aaaactgtag ggacgataaa taatggtggg attgatccca
    15901 caaggtcgca tattcgcatc aatcattgct tcgaagttag cccggttttg aaagcgcatt
    15961 tgtagccagg gcagctcctg aagcagctct tgctcttgac gctcttcata acggcgagaa
    16021 acgagaaagc ccaatcgcag cggtggtaat tcactgatag tcaggttttc cagttcctga
    16081 actcgtgcag atacatgctg tggggagata ataaaatcta actgacggtc aaaaagatta
    16141 tcgataacgc cgttttcgct gaactcaact ggctgggcgg tcacgccttc atacttatct
    16201 ccgagactaa tcaactgatc gaaaataatt gtcggataag tattatctat gccaatcaca
    16261 att
//
LOCUS       DAZRVY010000087        16199 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZRVY010000087 DAZRVY010000000
VERSION     DAZRVY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.087"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHXOX_002876"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHXOX_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR7006292.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHXOX_002877"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHXOX_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR7006293.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHXOX_002878"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHXOX_002878"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR7006294.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHXOX_002879"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHXOX_002879"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR7006295.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHXOX_002880"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHXOX_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHXOX_002881"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHXOX_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR7006296.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHXOX_002882"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHXOX_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR7006297.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHXOX_002883"
     CDS             7164..7604
                     /locus_tag="ACHXOX_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR7006298.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHXOX_002884"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHXOX_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR7006299.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHXOX_002885"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHXOX_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR7006300.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHXOX_002886"
     CDS             8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHXOX_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR7006301.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHXOX_002887"
     CDS             10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHXOX_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR7006302.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHXOX_002888"
     CDS             11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHXOX_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR7006303.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHXOX_002889"
     CDS             12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHXOX_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR7006304.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13149..13784
                     /gene="nth"
                     /locus_tag="ACHXOX_002890"
     CDS             13149..13784
                     /gene="nth"
                     /locus_tag="ACHXOX_002890"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR7006305.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHXOX_002891"
     CDS             14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHXOX_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR7006306.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16003..16122
                     /locus_tag="ACHXOX_002892"
                     /pseudo
     CDS             16003..16122
                     /locus_tag="ACHXOX_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
BASE COUNT         3757 a         4122 c         4136 g         4184 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca gcaacaggct
    10561 aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg ccgtcgaagc cgctattgcc
    10621 cgtgccaaag cgcgcaagct ggaacagcaa cagactaacg cagaaccaga agaacaggtt
    10681 gatccgcgca aagcggcagt tgccgcggct attgcccgcg ctcaggccaa aaaagccgcc
    10741 cagcagaagg ttgtaaacga ggactaaatg gtattcagaa tagctagctc cccttatacc
    10801 cataaccagc gccagacatc gcgcattatg ctgttggtgt tgctcgcagc cgtgccagga
    10861 atcgcagcgc aactgtggtt ttttggttgg ggtactctcg ttcagatcct gttggcatcg
    10921 gttagtactc tgttagccga agctctcgta ctcaaactac gcaagcagtc ggtagccgca
    10981 acgttgaaag ataactcagc attgctgaca ggcttattgc tggcggtaag tattcccccc
    11041 ctcgcgccat ggtggatggt cgtgctgggt acggtgtttg cggtgatcat cgctaaacag
    11101 ttgtatggcg gtctggggca aaacccgttt aatccggcaa tgattggtta tgtggtctta
    11161 ctgatctcct tcccggtgca gatgaccagc tggttaccgc cacatgaaat tgcggtcaac
    11221 atccctggtt ttatcgacgc catacaggtt attttcagcg ggcataccgc cagtggtggt
    11281 gatatgaaca cactacgctt aggtattgat ggcattagtc aggcgacacc gctggataca
    11341 tttaaaacct ctgtccgtgc cggtcattcg gttgaacaga ttatgcaata tccgatctac
    11401 agcggtattc tggcgggcgc tggttggcaa tgggtaaatc tcgcctggct ggctggcggc
    11461 ctgtggttgc tatggcagaa agcgattcgc tggcatattc ccctcagctt cttagtaacg
    11521 ctggcgttat gcgcaacgtt gggctggttg ttctcaccag aaacactggc agcaccgcaa
    11581 attcatctgc tgtctggtgc gaccatgctc ggcgcattct ttattttgac tgacccggtt
    11641 accgcttcta cgaccaatcg tggtcgtctt attttcggcg cgcttgcggg cttattagtc
    11701 tggttgatcc gcagtttcgg cggctatcct gacggcgtgg cttttgccgt cctgctggcg
    11761 aacatcacgg ttcctctgat cgattactac acgcgtccgc gcgtctacgg ccatcgcaaa
    11821 gggtaaacca tgctgaaaac tatccgaaaa cacggcatta cgttagcgct atttgcagcg
    11881 ggttcaacag ggttaactgc ggccatcaac cagatgacca aaacgacgat tgctgaacag
    11941 gccagtctgc aacaaaaggc attatttgat caggtgctgc cagccgaacg ctataacaat
    12001 acgctggcac agagttgcta tctggtaact gcgccagagt taggtaaagg tgagcatcgg
    12061 gtttacatcg ccaaacagga tgacaaaccg gtagccgccg ttctggaagc aaccgcgcca
    12121 gatggctatt ccggtgcgat tcagctgctg gtgggagccg attttaacgg cacggtactt
    12181 ggcacgcgcg tgacagagca ccacgaaacg ccagggcttg gcgataaaat cgaactgcgc
    12241 ctttctgact ggatcaccca ttttgcgggt aaaaaaatca gtggtgcgga tgatgcgcac
    12301 tgggcggtga agaaagatgg tggtgatttc gaccagttca ccggcgcgac gattactccc
    12361 cgcgcggtgg ttaatgcggt aaaacgcgcc ggattgtacg ctcagacgtt accggcacaa
    12421 ctttctcaac ttcctgcctg tggagaataa agcgtgagcg aaattaaaga cgttattgtt
    12481 caggggttgt ggaaaaacaa ctctgcgctg gtccagttgc tcggcctttg tcctctgttg
    12541 gcggtcacgt ccactgccac taacgctctg ggtttaggac ttgcgactac gctggtactg
    12601 acgctgacca acctgaccat ttcgacgctg cgtcactgga cgccagccga gatccgcatt
    12661 cccatttacg tgatgatcat cgcctcggtg gtcagcgctg tacagatgct gatcaacgcc
    12721 tacgcctttg gcctgtatca atcattaggg atttttattc cgctgattgt cactaactgt
    12781 atcgttgtgg gccgcgctga agccttcgcc gccaaaaaag gtccggcgct ttcggcactg
    12841 gacggctttt caattggtat gggcgcaacc tgcgccatgt tcgtgctggg ttcactacgc
    12901 gaaattatcg gcaatggcac attgtttgac ggtgcagatg cgctgttagg tagctgggca
    12961 aaagtattac gcgtggagat tttccacacc gactcccctt tcctgctggc gatgctgcca
    13021 ccaggtgcat ttattggcct gggactgatg ctggcaggaa aatacctgat tgatgaaaga
    13081 atgaaaaagc gccgtactga agcagctgcc gaacgtgcat tgccaaacgg tgaaacaggg
    13141 aatgtctgat gaacaaagca aaacgcctgg agatcctcac tcgcctgcgt gagaacaatc
    13201 ctcatcccac caccgagctt aatttcagtt cgccttttga attgctgatt gccgtactgc
    13261 tttccgctca ggcgaccgat gtcagtgtta ataaggcgac ggcgaaactt tacccggtgg
    13321 cgaatacgcc tgcagcgatg cttgaactgg gcgttgaagg ggttaaaacc tatatcaaaa
    13381 cgattgggct ttataacagc aaagcagaaa atatcatcaa aacctgccgt atcttactgg
    13441 agcagcataa tggcgaggtt ccggaagatc gtgctgcgct tgaagccctg cccggcgtag
    13501 gtcgtaaaac agccaacgtc gtattaaaca ctgcattcgg ctggccgact attgctgtcg
    13561 acacgcacat tttccgcgtt tgtaatcgta ctcaatttgc gccggggaaa aacgtcgaac
    13621 aggtagaaga aaagctactg aaagtggttc cggcagagtt taaagtcgac tgccaccatt
    13681 ggttgatcct gcacgggcgt tatacctgca ttgcccgcaa gccccgctgt ggctcttgta
    13741 ttattgaaga tctttgtgaa tacaaagaga aagttgacat ctgaagaaaa ggggtaacac
    13801 cgattacccc attgataacc tttctttatc ctcttttaaa acatcttttt aacgtgaatg
    13861 atgccattgc ttagcgttat catcaggtaa tccgtttgcg gataaccaaa aatgcaggtt
    13921 aattgttttt ttaatagcga aattttctat tcattcgtga tcaagatcac gatgctgtcg
    13981 caatgtaaaa acattgcaag aatcaggtta aaataattta aatttacaca cgcaacaaat
    14041 attgacctac aaaacattac actggctatt tttcagaaac tggactatct cactagtaaa
    14101 aacgccaaat agcagaacat atcgccgtta agccatcacc ccccctctca gtgcagtgaa
    14161 aaaatctgcc attacgtttt ttgaaaaatt taacgctgga taacatttcc cggaatggtt
    14221 gaattccccg cctcagttat atgtaacaga ttattacaaa ggacttgtct gaaagtgcaa
    14281 gatagtgaac attacctgcc gtttcccctc ccactataac aattgcgcgt atgtttttta
    14341 gacataacgc gagaaagccc ccccgttaat atgggatgta aaaaaagagg taaaagtgtc
    14401 cactgcaaac caaaaaccaa ctgaaagcgt cagtttgaac gctttcaaac aaccgaaggc
    14461 gttctatctc atcttctcga ttgagttatg ggaacgtttt ggttattacg gcctacaagg
    14521 aattatggct gtttacctgg ttaaacaact gggtatgtct gaagcggatt caatcaccct
    14581 tttctcttcc tttagtgccc tggtttatgg tctggtcgct atcggcggct ggttaggtga
    14641 caaggtactg ggtactaaac gcgtaattat gctcggcgct attgtgctgg cgattggtta
    14701 tgctctggtt gcctggtctg gtcacgacgc cggtatcgtt tatatgggta tggcggctat
    14761 tgcggtcggt aacggcctgt ttaaagctaa cccatcttct ctgctttcta catgctatga
    14821 gaaaaacgac ccgcgtctgg acggtgcatt caccatgtac tacatgtccg tcaacatcgg
    14881 ctctttcttc tctatgattg ctacgccgtg gctggccgcg aaatacggct ggagtgttgc
    14941 gtttgcgttg agcgttgtag gcctgctgat cactatcgtt aacttcgcct tctgccaacg
    15001 ctgggttaaa cagtacggtt caaaaccaga cttcgagcct atcaactacc gtaacctgct
    15061 gctgaccatt attggtgttg tggcactgat cgctatcgcc acctggctgc tgcacaatca
    15121 ggaagttgcg cgtatggcgc tgggcgttgt tgccttcggt atcgtggtta tcttcggtaa
    15181 agaagccttc gcgatgaaag gtgctgcgcg tcgtaaaatg atcgttgcct tcatcctgat
    15241 gctcgaagcc attatcttct tcgtgctgta cagccagatg ccaacgtcac tgaacttctt
    15301 tgcgattcgt aacgttgagc actccattct gggtctggcc gtagaacctg agcagtatca
    15361 ggcactgaac ccgttctgga tcatcatcgg tagtccgatt ctggccgcta tctataacaa
    15421 gatgggcgat accctgccga tgccaaccaa gtttgcaatc ggcatggtga tgtgttctgg
    15481 tgcgttcctg attctgccgc tgggtgcgaa attcgcgtct gacgctggta tcgtgtctgt
    15541 aagctggctg gtcgcaagct atggcctgca gagcatcggg gaactgatga tctctggtct
    15601 gggtctggca atggttgcac aactcgttcc gcagcgtctg atgggcttca ttatgggtag
    15661 ctggttcctg accactgccg gtgcaaacct gattggtggt tatgttgcgg gtatgatggc
    15721 tgtgccggat aacgttaccg acccgctgat gtcactggaa gtttatggtc gcgtattctt
    15781 gcagattggt gtcgctactg ccattattgc agtactgatg ctgctgaccg cgcctaaact
    15841 gcaccgtatg acgcaggatg acgctgcaga caaagcggcg aaagctgctg tagcgtaaaa
    15901 ttcagggaaa ctcttttaca agccgctaac ttttcgttag cggctttttt ttgttcagca
    15961 acgcactacc atactttaaa ccacagacaa aaggagttac cgatgaaatt gttctacaaa
    16021 ccgggtgcct gctctctcgc ttcccatatc accctgcgtg agagcggaaa ggattttacc
    16081 ctcgtcagtg tggatttaat gaaaaaacgt ctcgaaaacg gtgtaatgcc gatcagttaa
    16141 ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact tgtttccgg
//
LOCUS       DAZRVY010000088        16093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZRVY010000088 DAZRVY010000000
VERSION     DAZRVY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.088"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(176..328)
                     /locus_tag="ACHXOX_002893"
     CDS             complement(176..328)
                     /locus_tag="ACHXOX_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7006307.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(593..1327)
                     /gene="cysH"
                     /locus_tag="ACHXOX_002894"
     CDS             complement(593..1327)
                     /gene="cysH"
                     /locus_tag="ACHXOX_002894"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR7006308.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1402..3114)
                     /gene="cysI"
                     /locus_tag="ACHXOX_002895"
     CDS             complement(1402..3114)
                     /gene="cysI"
                     /locus_tag="ACHXOX_002895"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR7006309.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3114..4913)
                     /gene="cysJ"
                     /locus_tag="ACHXOX_002896"
     CDS             complement(3114..4913)
                     /gene="cysJ"
                     /locus_tag="ACHXOX_002896"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR7006310.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5232..5594
                     /gene="queD"
                     /locus_tag="ACHXOX_002897"
     CDS             5232..5594
                     /gene="queD"
                     /locus_tag="ACHXOX_002897"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR7006311.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5672..6943
                     /locus_tag="ACHXOX_002898"
     CDS             5672..6943
                     /locus_tag="ACHXOX_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7006312.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6934..7194
                     /locus_tag="ACHXOX_002899"
     CDS             6934..7194
                     /locus_tag="ACHXOX_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR7006313.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7211..7786
                     /locus_tag="ACHXOX_002900"
     CDS             7211..7786
                     /locus_tag="ACHXOX_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR7006314.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7934..8794)
                     /locus_tag="ACHXOX_002901"
     CDS             complement(7934..8794)
                     /locus_tag="ACHXOX_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR7006315.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8791..9570)
                     /locus_tag="ACHXOX_002902"
     CDS             complement(8791..9570)
                     /locus_tag="ACHXOX_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR7006316.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9548..10498)
                     /locus_tag="ACHXOX_002903"
                     /pseudo
     CDS             complement(9548..10498)
                     /locus_tag="ACHXOX_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10485..11918)
                     /locus_tag="ACHXOX_002904"
     CDS             complement(10485..11918)
                     /locus_tag="ACHXOX_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR7006317.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11988..12804)
                     /locus_tag="ACHXOX_002905"
                     /pseudo
     CDS             complement(11988..12804)
                     /locus_tag="ACHXOX_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13122..14399
                     /locus_tag="ACHXOX_002906"
     CDS             13122..14399
                     /locus_tag="ACHXOX_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR7006318.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14426..15912
                     /locus_tag="ACHXOX_002907"
                     /pseudo
     CDS             14426..15912
                     /locus_tag="ACHXOX_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3808 a         4231 c         4040 g         4014 t
ORIGIN      
        1 atatatatca ttcaataaaa tatttacatt atgaataatc aaataatttt catccgtaat
       61 atatatgaat aataaacata ggcataatat tcacataaat aatataaatt aaaaaagggt
      121 gcaggagacc aaaccatcac aattcagtac gcggggtttt acccccgcgt ggtggctact
      181 tcttcgattc gtaagcgaga actgccttaa actccatacc acgttctctg atactcaact
      241 cacacaacga gtcgcctacc atcagcgtga atcccagtac tgtaaaacac agcactatga
      301 ttgccacaag ggcatatttt gtcagcatgt cttgcctccc tgcaaagaag agactaacat
      361 tcgaattgtt tgggttcgaa cgctggcctc aggttgatag aaatatcgcc tggggctttt
      421 gtccatctgg aacctcgcga atgcttaacg ccagacagcc tcaagcaccc gacgccattc
      481 tatacctgat aattcttcca gcggcgttca cgtttaaaat caggaatact gctcgcaaaa
      541 taaaaacgcc cccatcattt ctgacagagg cgtttaattt atccggcaat atttaccctt
      601 cgtgcaatcc acattccctt tttaagccaa agaaacgtgt ttcttcttcc gacatgccag
      661 gttcccattt acgggttgta tgggtatcac cgaccgataa atatccttca tcccataatg
      721 ggtgatattt caggccatgt ttttgcaggt actgataaat agttcggtta tcccagtcga
      781 taatcggcag cactttaaat acgccacgct gaattgccag caccggtaaa ttggcgcgac
      841 tgccggattg ttcacggcgc aggccagcaa accaggtttg cgcgttaagt tctttcagag
      901 cccggttcat cggttcgact ttgttgatgt cattgtactt ttcaatgcct tcaacgccct
      961 gctcccacag ttttccgtag cgtgcttcct gccaggctgc gctttcggta gcacggtaca
     1021 ctttcaggtt gagcttgagt ttgtccgtta actcgtcaat aaagcggtag gtttccggga
     1081 acaagtaacc cgtatcggtg aggatcaccg gaatatccgg gcgaatttga ttcaccagat
     1141 gcaggctcac cgccgcctga atgccaaagc tggaagaaag tacatattca ccgggcagat
     1201 tatccagcgc ccaggctacg cggccttcag cgtccagttt ttccagttcg gcgttagttt
     1261 ccgccagcgc cagaatacga tctaccttcg gcagttcgtt cagggcgttt agatcgagtt
     1321 tggacataga ttcctcactg tttgccttgc ctgatgcgac gctcacgcgt tcttatcagg
     1381 cctacaagac cgggctgatg gttaatccca caaatcgcgc gccggatcga gcaccgggcg
     1441 aatgatgccc gcacgcaccg taaagtcgcc gaagccttca cccgcttcgc gctctttcgc
     1501 ccagcgccct atcagttcat caagcgacgc caggatttcc ggctcggtga tgttttcttt
     1561 atacatccgt gggatacgtg tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc
     1621 cggcgcttta cccaccaggc ccacttccgc cagcatcgcg cgaccacaac cgttcgggca
     1681 gcctgttaca cgcatcacga tatgctcatc gctgacacca tgtttcgcca ttaaattatc
     1741 gatgttgtcg ataaaagacg gcaggaaacg ctctgcttcc gccatcgcca gcgggcaagt
     1801 cgggaatgac acgcaggcca tcgagttttc acgctgcggc gtgacggcat tcattaaccc
     1861 gctctctttg gcgatcttct cgatcttcgc tttctcgctt tccggtacac cggtgatgat
     1921 cagattctgg ttcgccgtaa tgcggaaatc gcctttgtgg atcttcgcga tctccagcag
     1981 gccggttttc agcggacgcc ccggataatc aaggatgcga ccattttcga taaacagcgt
     2041 caggtgccag ttatcatcaa tgcccttaac ccagccaata cgatcgcctc gtccggtgaa
     2101 ctcatatgga cggatcggtt caaatttgat ccccgcgcga cgctccactt ccgctttaaa
     2161 cgtctcaacc cccacgcgct ccagcgtgta tttggttttg gcatttttac gatcggttcg
     2221 gttaccccag tcacgctgag ttgtcacgac ggcttcggcc accgccagcg tatgctccag
     2281 cggcagatag ccaaactcgc tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat
     2341 agaaagccca ccgcccacca gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc
     2401 cacgaagttc atgtcgttgg catgcagatc gatatcgttc tgcggcggga tcactaccgt
     2461 ggttttgaac ttacgcggca ggtaggtctg accaaggatc ggttcttcat cagtagtggc
     2521 gactttttcc tggtcgagcc agatctccgc atacgcgcgg gtacgaggca acagatgctc
     2581 agaaatcttc ttcgcccact cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga
     2641 ggtgcagagt acgttacggt tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga
     2701 gtgcagcatc tggtgcaccg gtttgacgtt ctttttcaga atgccgtgga actgaaacgt
     2761 ctggcggttg gtcaggcgaa tgctgccata gatggtgttt tcaccggcaa atttgtcgat
     2821 cgcctgccac tgtttagtgg taatcacccc acccggcaga cgacagcgaa gcagcatcgc
     2881 gtggcgcggc tccagcttct gttcagcacg ttcggcacgg atgtcgcggt catcctgctg
     2941 atacatgccg tggaagcgga tcagcaggaa gttgtcgccc ttaaagccgc cggtcagacc
     3001 gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg ctttcaagct tcatgcgctc
     3061 ggcgtctgtc agttttcctt cgaccactaa aggccctgga tgtttttcgc tcattagtag
     3121 acatctcgct gataacggcg ctctacgcgc agctcactta aaaattcatc cgccgcttcg
     3181 gtgtccatgc caccaaattc ggcaatcact tccagaagtg cctgctcaac gtctttcgcc
     3241 atgcgattag cgtcgccgca gacataaatg tgggcaccgt cattgatcca gcgccacagc
     3301 tccgcgccct gttcgcgcag tttgtcttgt acataaactt tttctttttg gtcgcgcgac
     3361 caggcaagat cgatacgcgt cagcacgccc tctttgacgt agcgctgcca ctccacctgg
     3421 tagaggaaat cttccgtaaa gtgcgggttg ccaaagaaca gccagttttt acccggcgct
     3481 tcgtcggcgg cgcgttgctg cataaaggca cggaacggcg cgatgccggt accggggcca
     3541 atcataatca ctggcgtttg cgggttagcg ggcaggcgga aattgtcgtt atgttcgata
     3601 aatacgcgga cttcgccctc ttcttccacg cgatccgcga ggaagctgga ggcaccaccg
     3661 gcacgggcgc ggccttccac gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc
     3721 tcgacttccg cctgcgagga ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcagg
     3781 ttaatcagcg cttcggcatc cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc
     3841 gtggtcgcgg cgtaatgctg taacttcgct ttatcgccca ccagcggcag cagcgtttcg
     3901 ctgcgggtaa gcgtggcata gttctcaaca atgttggcgg tgttgacggt cagttcgaag
     3961 tgccactgta gcgcttcgtt cagaggcaac gttttgccct cgacggtgac aggttcatcg
     4021 cctttcagcc acagcagttc gacaagttct ttcaccagtg ccgggtcgtt ctgataccag
     4081 acacccagcg cgtcacccgg ctggtaacgc aggcctgaat cgcctaagtc aatttcgata
     4141 tgacgaacgt ctttttcaga gttacgcccg gtaattttct ggttaaccga aaggctcgcc
     4201 accagcggcg cgtctttgct gtacgggctg gtgtggattt catttaccgc gccagtagcg
     4261 acggattgcg aaggtgccgc gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg
     4321 cgccactcgc tggcagcagc ctggtattca acatcggcat cgacacggtc gagcaggcgt
     4381 tcaccaccca gttccgccag cttgctgtcg aaatctttcc cggactggca gaaaaattca
     4441 taagagctat cgccgaggct aaacacggca aacgcggtgt tttccagctt tggcgctttt
     4501 ttggagaaca ggaacttatg cagcgcgacg gcttcttccg gcggttcccc ttccccttgc
     4561 gttgacgtca ctacgatgag cagtttttcg ctggcgattt gtttgaattt atagtcgccc
     4621 gcgttcacca gcttaacgtt cagttttgct gctaataaat catcgcgtaa tgcttcagca
     4681 acccggcgcg cattgccggt ttgcgaggcg gagataatag ttatacccgg catttctgcg
     4741 gctggcgctg gcgtcgctgc aagcgcagca ggctgctgat tgagtacgcc ccagaaatag
     4801 ccagaaaccc aggcaagctg ggtaggcgtt aaatcggtcg tggccgcctg aaggcgtgcc
     4861 agttgctccg ggttcaacgg aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt
     4921 tatgttccag taagcaaagc tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa
     4981 gataaggtta acggggtgaa cggtgtggat taaagacggg atagcgataa ctaataacca
     5041 aatcgactaa cctgttttag caatagtctt taacaacaaa atagattaac caacccaatg
     5101 aaaaacaaat gaatttagcc aatcattaag ataaatcagc gattttgcgc aacaaagtcg
     5161 ttttagataa tgcgaaaaaa cagcctttcc ggtactctac ggcggtttta tcgtcctgta
     5221 gagaaattat gatgtccacc acgttattta aagatttcac cttcgaagcc gctcaccgct
     5281 taccacacgt cccggaaggg cataaatgtg gtcgcctgca cgggcattcc tttatggtgc
     5341 gactggaaat taccggggaa gtcgatccgc atacgggctg gattatcgat ttcgctgaac
     5401 tgaaagcggc gtttaagcca acttatgagc gcctcgatca ccattatctc aatgatattc
     5461 caggtctgga aaacccaacc agcgaagttt tagcaaaatg gatttgggat caggttaaac
     5521 ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac ctgcaccgca gggtgtatct
     5581 atcgcggcga atgataagag tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag
     5641 ggtgttgtgt tctggatatc tggggcatga catggaagac gactgcgaca ttattattat
     5701 tggtgccggt attgcaggca ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt
     5761 tttgttactg gaacgcgctg aaatccccgg cagcaaaaat ctttccggcg ggcggttata
     5821 tacccatgca ctcgcggaac tcctcccgca atttcatctg accgcgcctc ttgaacgacg
     5881 catcactcac gaaagccttt ccctgttaac gcccgatggc gtaacgacgt tttccagctt
     5941 acagcccggc ggtgaatcct ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc
     6001 cgaagccgaa aaagaaggtg tcgaatgcat ccccggagcg acggtggatg cactgtatga
     6061 agaaaacggc agagtctgtg gcgttatttg tggtgacgat attctccgcg cccgttatgt
     6121 ggtgctggca gaaggtgcca acagcgtcct ggctgaacgt cacgggttag tgactcgtcc
     6181 tgctggcgaa gcgatggcgt tggggatcaa agaagtgctg tcgctggaaa catccgctat
     6241 tgaagaacgt tttcatctgg agaataacga aggcgcagcg ttgctgttca gcggcggaat
     6301 ctgtgatgac ttacccggcg gcgcatttct ttatactaat caacaaacgc tctcgttagg
     6361 gattgtttgc ccgctctctt cccttacgca aagtcgtgtt ccggcaagcg agctgctgac
     6421 tcgctttaaa gcgcatccgg cagtgcgccc gcttatcaaa aacacggaat cactggagta
     6481 tggtgcgcat ctggagccag aaggtggctt gcacagtatg ccggtgcaat acgccggtaa
     6541 cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg
     6601 cggcatggat atggcgctga ctggcgcaca ggcggcggca caaacgctaa taagcgcctg
     6661 ccagcaccgc gagccgcaaa atctgtttgc gctttatcat cacaacgtag agcgcagcct
     6721 gctatgggat gttctacaac gttatcagca tgttccggcg cttttgcaac gccctggctg
     6781 gtatcgggcg tggcctgcgt taatgcagga tatttcccgc gatttatggg atcagggtga
     6841 taaacctgtt ccaccgctgc gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg
     6901 gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg tagcccataa tctctggcgc
     6961 gttgctgatg cgccgcacat tgttccggct gactccgttg agcgtcagat ggtacaacgg
     7021 ttgattaacg cctgtccggc aggtcttttt tcgctcacac cggaaggtga cttacgcgtt
     7081 gattatcacg gctgcctgga gtgtggcacc tgccgtttgc tgtgcgacga atccacacta
     7141 caacagtggc gctatccgcc ttccggattc ggcatcacct accgctttgg ataaataata
     7201 aggataattt atgcccctct tacacctact ccgccagaat ccggtgattg ctgccgttaa
     7261 agataatgcc agcctgcaac tggctatcga ttctgaatgc cagtttattt ccgtgttgta
     7321 cggcaatatc tgcaccatca gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc
     7381 ttttattcat gttgatttgc tggaaggcgc gtcaaataaa gaagtcgtta ttcagttttt
     7441 gaaactggtg accgaggcgg acggcattat cagtaccaaa gcctcaatgt tgaaagcggc
     7501 aagagcggaa ggttttttct gtattcatcg cctgtttatt gttgattcaa tttcgtttca
     7561 caacattgat aagcaagttg cgcaatcgaa tccggattgt attgagatcc tgccaggctg
     7621 tatgcccaaa gtgctgggct gggtgacaga gaaaatccgc caaccgctga ttgccggtgg
     7681 gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc
     7741 caccacgaat accggggtct ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa
     7801 ccaattgaat ttggtttgat ttgtaggccg cacgccacat ccgacattca gcgcctgatg
     7861 cgacgcttga cgcgtcttat caggcctaca agtccccagg ccagaaccgt aggcaggatc
     7921 atccggcaac acgttattgg caatctgcgt gaatgtttgt caccagtgcc tccagcacca
     7981 ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat
     8041 gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct gctgcgccag
     8101 aagcgccaac aacaatacac acctcaggtg ccagcagatg tccggaaata ccaatcactt
     8161 tttcagcatc aacgccaccg ttcatcactc gcgcccgact gtagcccact tcggctccca
     8221 gcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgc cccaccacca
     8281 gcacccgtct ggcctcagct aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa
     8341 ccagccagtc cggaagcgca cccgggacaa tgttcagttg ttgcattcca gagggcaacg
     8401 tggcgttttt cgctgctccc gcctgacgcg ccagggaaag gcatagcggg cgtttttctg
     8461 tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt acggttggaa
     8521 tatctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc agccgggtcg
     8581 acagttcatc gccaaacgtt cccggcggaa agagaacaac gtccgcaggc gttcgctgcc
     8641 actgttccac caacgcatcc agcacctgtt ccgccactac aggctgaggt tcaatctgcc
     8701 agtgcgccag ggtacaaccg ctgaaatcct gcgccgccag ccagcttgct attgcggcat
     8761 tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc gtaaatagtc
     8821 ctgccatagc ttttgagctt tttctgctac cgtttgccca tcaatcagcg tcgctccgcg
     8881 tcgttgctct gctcttgcca gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt
     8941 tttacggata atttctgctt tccccgcagc cattcgctgg cgcattcccg gtactggcaa
     9001 ggccacttca ccgcactgac gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg
     9061 caacccatgt tcagtacgct gttcgagggt gataaacagc gcgtcgagag tgaaacgctc
     9121 cacctgggtg aagcagggcc agcccagcat ttccgccagc aaaaatggcg tttgcccatt
     9181 ctgcccttcg ctgctttggc agccggtaat tatcagatcc agcggattct gatgctgcca
     9241 ttcggcaata tggcgagcga caaattccgg cgcaaagcgc agatctgccg ccgtctccag
     9301 caatacagct tcctcaaacc caagagccat gagatagcgc agccagtgca atgcccgttc
     9361 atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg ttttttctct gcgccaacag
     9421 cagcgcggca gcggcctgtt catcagcacc gagtaaactt cgcagtagcg aaatatccgg
     9481 tccgctttta ccctgagccg ccgcctgcca ctctttttcc gccagcattc cggcatccgg
     9541 ttcggcttta aacgctaaca gaatgttcat tcgcacctcc tacatttccc gccgccacca
     9601 gcgggagggc tttagtttct ggtgcccata gccaggtcac aacaaaatca accaacaaca
     9661 ctgtcgccag gagcaataag gtgacttgca ttccccactg cgccagcacc cacggcagca
     9721 ggccagtact taccgccgcg ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg
     9781 agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta ctgactgccg
     9841 aaatggtggt gctgaagaga acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac
     9901 aggccatcac taccagcgtt gccgccagca gcaaaaaact tcccagcaaa aatttgcgat
     9961 gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc cacaattaac aacgcattaa
    10021 gcatcaggct ggcagtcagc gcatcttcca gaccaatagt ctgggcgata gttggcagcc
    10081 aggtataaat cacaaaccat gggattacga ggcagacaaa gaagacgctg ttaaacgccg
    10141 tgcgccgcca gtaacgcgaa gagaacaagg ttttgatgtg tttatgggtc gccgttacca
    10201 cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg cacgatagcg tgagcttctg
    10261 caaaacgccc ctggcgcagt agccagcgtg gtgattctgg cgttccccag cgtaataacg
    10321 taatcaacaa cgcgggcaga gctgccgatg ccagtagcca gcgccaggcc tccgggtttt
    10381 cggaaataaa gtgatgtccg gcaatacttg ccagcacata gcctacggtc cacaccacgc
    10441 tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga aaattcagcc agcaaggtta
    10501 atgccattag gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt
    10561 tccagtttgc tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg
    10621 ccgaggcgaa tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt
    10681 tcctcttccg gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg
    10741 ttctgatagc tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac
    10801 tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg
    10861 gacacttcag tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc
    10921 actttatccg gtccccagtt caggttgtta aaccaggttt cgatcagctt gctgtccacg
    10981 cgctggcatt gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca
    11041 atgcgagggt taccttcagc cataaagatc agcacgcatt ttccgtctgc aaaatgggtg
    11101 aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc
    11161 accatgactt cacgcaggat gttgaagtcg gttttcatgt cttccaggat atagccgtag
    11221 aagaggttgt tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcaa
    11281 agtgcacctt cgttaccgat gatgatgtgg cgaatgtccg ggccagccgc gcggcgtggc
    11341 acgtttttaa tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata
    11401 tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaagccg
    11461 cccatctgcg ccagcggctt tgactgcgga gaatgccccg tggtgtaacc tttttcacgc
    11521 aacgcatttt ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg catattctca
    11581 atatcaatat taatgatttg attcatggcg gagccgtcaa gcaccaccga gttttccaca
    11641 acggtttcca gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg
    11701 tgcgcattca taaaattcag cacacgggat acttgctctg tggaaccgag ttttacgacc
    11761 gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg aaaacggtca
    11821 atactgttct tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc
    11881 tttagttggt cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa
    11941 aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa
    12001 taaccaccat tgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg
    12061 aatacagctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg aatatgatca
    12121 agaacgcgct gattggtttc tggattactg cgtgtcgcca gcgtaatatc tgttgcataa
    12181 tagccaggag caataccatt tacctgaata ttatattgac ctagttcatc acaataagct
    12241 ttggtgaatc cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct
    12301 aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt
    12361 tttgcagctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc
    12421 cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc attgtttacc
    12481 agaatatcaa ctgtaccgaa acgctcacag caagcagcga taatcttctg cggcgcgcct
    12541 tctgcggtga tatccacctg catgaagtcc acctcaacac cctgtttttc aaacatttcc
    12601 tttgtttcgc cgttatcttt caatcatttc ctttgtttcg ccgttatctt tgacgaaact
    12661 aggaataaag atatttgcgc cagctttggc caacgccatg gcaaatgcct ggcctaaacc
    12721 gctattccca ccggtaacaa ttgcggtttt acctttcagt gagaaaaaat ccattgagaa
    12781 cgcattgaga gattcgattg acatagttag ctccaatttc cgtaaggcaa aaaaaagaaa
    12841 ggtaacttcc cccgcgagag ggaagttacc tttctcatta tctctggtaa ctggagataa
    12901 tttttagcat aacggcataa ctgctaatgt gatcgtaatc acagtgtgat attcgtattg
    12961 tcaaatagtt ttaaaaagca gaagcgtgat tactctcaca caacacctct gctttccatg
    13021 ttatttctta ttgcaattac tagagacctg agaaaagtaa tccacctgaa agggcggctt
    13081 acttttctct tttttttgtc tattacaaat gggaatacga tatgcaacac aactcatatc
    13141 gccgttggat aaccctcgcg ataattagtt ttagcggcgg cgttagtttt gacctggctt
    13201 atttacgtta tatttatcaa attcccatgg cgaaatttat gggattcagc aataccgaga
    13261 taggtttaat aatgagtacc tttggtattg cggctattat tctttatgcc cccagcggcg
    13321 ttattgccga taaattttca caccgcaaaa tgattacttc cgcgatgatc attaccggat
    13381 tactgggtct gttaatggca acgtatccac cgctgtgggt aatgctctgt attcaggtcg
    13441 cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc
    13501 tgggcgatca tagtgagcaa gggaaaatta tgggctggat ggaagggctg cgcggcgtcg
    13561 gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc tcgctttgca ccggatgaca
    13621 gcgccagcct gaaaacagtc attatcatct acagtgtggt ttacatcttg ttggggattc
    13681 tgtgctggtt ttttgttagc gataacaaca acctgcgcag tgccaataac gaagaaaaac
    13741 agtcattcca gcttagcgac atccttgccg ttttgcgtat cagcaccacc tggtattgca
    13801 gcatggtgat ttttggtgtc ttcacaatct acgccattct gagttactcc accaactatc
    13861 tgaccgaaat gtacggcatg tcgctggtgg cagcgagcta catggggatt gtgatcaaca
    13921 aaatcttccg cgcgctgtgc ggcccacttg gaggcattat caccacctac agtaaagtga
    13981 aatcccctac ccgcgtgatt caaatccttt ccatcatcgg tttgctggcg ttgacagccc
    14041 tgctcgtcac gaactctaac ccccaatcgg tcgcgatggg gattggcctg attttactgc
    14101 tgggattcac ctgttacgcc tcacgcgggc tgtactgggc ctgccccggc gaagcgagaa
    14161 caccgtctta cattatgggc accacggtag gtatttgttc ggtgattgga ttcctgccgg
    14221 atgtcttcgt ttacccgatt atcggccact ggcaagacac cctgcccgct gcagaagcct
    14281 accgcaatat gtggctgatg ggcatggcgg cgctcggcat ggtgattgtc tttacctttt
    14341 tgctgttcca aaaaattcgt actgctgata gcgcccccgc aatggctagc agcaagtaag
    14401 ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac atcataggga ttgatggcgg
    14461 aagtcagagc acaaaagtgg tgatgtacga tctggaaggt aatgtggttt gcgaaggtaa
    14521 aggcttatta cagccgatgc acacgcctga tgccgatact gcagaacatc ctgacgacga
    14581 tttatgggca tcattatgtt ttgcaggtca cgatttgatg agccagtttg ccgggaataa
    14641 agaagatatt gttggcatcg gcctgggctc catccgttgc tgccgtgcgt tattgaaagc
    14701 cgacggcacg ccagctgcgc cgttgattag ctggcaggat gcacgcgtta cacgccctta
    14761 cgaacatacg aatcctgacg tggcgtatgt aacctctttt tcgggttatc tgacgcatcg
    14821 cttaaccggc gaatttaaag ataatatcgc caactatttt ggtcagtggc cggtggatta
    14881 taagacctgg gcatggagcg aagatgccgc agtcatggag aagtttaata tcccccgtca
    14941 tatgctgttt gatgtgcaaa tgccaggcac tgttcttggg catatcacac ctcaagccgc
    15001 gctggcgacg catttcccgg caggactgcc ggttgtttgt accaccagtg ataaaccggt
    15061 agaagctctg ggagccggat tactggatga tgaaactgcg gtaatttctc ttggcactta
    15121 catcgcattg atgatgaacg gcaaagcctt gccgaaagat ccggtggcgt actggccgat
    15181 tatgtcttct attccgcaaa cattgctgta tgaaggttac ggtattcgca aaggcatgtg
    15241 gacggtgagc tggctgcgcg acatgttagg cgagtcgtta attcaggatg ccagggcgca
    15301 ggatctttca ccggaagatt tactcaacaa gaaagcctct agtgtgccac ctggctgtaa
    15361 tgggctgatg acggtgctgg actggctgac caatccgtgg gaaccgtaca aacgcgggat
    15421 tatgatcggc tttgattcca gcatggatta cgcatggata tatcgttcga tattggaaag
    15481 cgtggcgctg acgctgaaga acaattacga caatatgtgt aatgaaatga atcactttgc
    15541 gaaacatgta atcattactg gcggcggttc gaacagcgat ctgtttatgc agatttttgc
    15601 cgacgtgttc aaccttccgg cacgccgtaa cgccattaac ggttgtgcaa gcctgggggc
    15661 agcgattaat acaggggtag gtctggggct atacccggat tacgcaacgg ctgtcgataa
    15721 aatggttcgc gtgaaagata tctttatacc gattgagagc aatgccaaac gctacgacgc
    15781 gatgaataaa ggcattttca aagacctaac caaacatact gatgtgatcc tgaaaaaatc
    15841 gtatgaagtg atgcatgggg aattggggaa ttggggaatg cggattcgat ccagagctgg
    15901 tcgaatgcgt gaataaggta ggccgggcgg aaaactgccc ggcctgaaca tacctgaatg
    15961 gttatccccg ctggcgcggg gaacataagt tcgacaactt aaaaaatgat aacaagtccg
    16021 gtttatcccc gctgatgcag ggaacataat aaaaacttta actagttcgg tttatccctg
    16081 tttttacaag gaa
//
LOCUS       DAZRVY010000089        16049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZRVY010000089 DAZRVY010000000
VERSION     DAZRVY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.089"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHXOX_002908"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHXOX_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR7006319.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHXOX_002909"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHXOX_002909"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR7006320.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHXOX_002910"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHXOX_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR7006321.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHXOX_002911"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHXOX_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR7006322.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHXOX_002912"
     CDS             6397..6507
                     /locus_tag="ACHXOX_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR7006323.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHXOX_002913"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHXOX_002913"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR7006324.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHXOX_002914"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHXOX_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR7006325.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHXOX_002915"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHXOX_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHXOX_002916"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHXOX_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR7006326.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHXOX_002917"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHXOX_002917"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR7006327.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHXOX_002918"
     CDS             13514..14446
                     /locus_tag="ACHXOX_002918"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR7006328.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHXOX_002919"
     CDS             complement(14487..15917)
                     /locus_tag="ACHXOX_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR7006329.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4097 c         3943 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacggg
//
LOCUS       DAZRVY010000090        15961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZRVY010000090 DAZRVY010000000
VERSION     DAZRVY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.090"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXOX_002920"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXOX_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR7006330.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHXOX_002921"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHXOX_002921"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR7006331.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHXOX_002922"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHXOX_002922"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR7006332.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHXOX_002923"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHXOX_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHXOX_002924"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHXOX_002924"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR7006333.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHXOX_002925"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHXOX_002925"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR7006334.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHXOX_002926"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHXOX_002926"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR7006335.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHXOX_002927"
     CDS             8229..8939
                     /locus_tag="ACHXOX_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR7006336.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHXOX_002928"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHXOX_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR7006337.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHXOX_002929"
     CDS             9636..10343
                     /locus_tag="ACHXOX_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR7006338.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHXOX_002930"
     CDS             10343..10891
                     /locus_tag="ACHXOX_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR7006339.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHXOX_002931"
     CDS             10901..12067
                     /locus_tag="ACHXOX_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7006340.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHXOX_002932"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHXOX_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHXOX_002933"
     CDS             13576..14229
                     /locus_tag="ACHXOX_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7006341.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHXOX_002934"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHXOX_002934"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR7006342.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="ACHXOX_002935"
     CDS             complement(15612..>15961)
                     /locus_tag="ACHXOX_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR7006343.1"
                     /translation="FYALVPFGFILAAPEQNALAGGRLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3698 a         4344 c         4165 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccggccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAZRVY010000091        15931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZRVY010000091 DAZRVY010000000
VERSION     DAZRVY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.091"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHXOX_002936"
     CDS             <1..173
                     /locus_tag="ACHXOX_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR7006344.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHXOX_002937"
     CDS             complement(345..674)
                     /locus_tag="ACHXOX_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR7006345.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHXOX_002938"
     CDS             complement(679..864)
                     /locus_tag="ACHXOX_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR7006346.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHXOX_002939"
     CDS             complement(861..3500)
                     /locus_tag="ACHXOX_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR7006347.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHXOX_002940"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHXOX_002940"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR7006348.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHXOX_002941"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHXOX_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR7006349.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHXOX_002942"
     CDS             complement(5227..5493)
                     /locus_tag="ACHXOX_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR7006350.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHXOX_002943"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHXOX_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR7006351.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="ACHXOX_002944"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="ACHXOX_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR7006352.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="ACHXOX_002945"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="ACHXOX_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR7006353.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11951..12865
                     /gene="sitA"
                     /locus_tag="ACHXOX_002946"
     CDS             11951..12865
                     /gene="sitA"
                     /locus_tag="ACHXOX_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR7006354.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12865..13692
                     /gene="sitB"
                     /locus_tag="ACHXOX_002947"
     CDS             12865..13692
                     /gene="sitB"
                     /locus_tag="ACHXOX_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR7006355.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13689..14546
                     /gene="sitC"
                     /locus_tag="ACHXOX_002948"
     CDS             13689..14546
                     /gene="sitC"
                     /locus_tag="ACHXOX_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR7006356.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14543..15400
                     /gene="sitD"
                     /locus_tag="ACHXOX_002949"
     CDS             14543..15400
                     /gene="sitD"
                     /locus_tag="ACHXOX_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR7006357.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15601..>15931)
                     /locus_tag="ACHXOX_002950"
     CDS             complement(15601..>15931)
                     /locus_tag="ACHXOX_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR7006358.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3985 a         4023 c         3830 g         4093 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 atatcagatt tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa
    11521 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11581 cctgaacaag taagggcaaa aatcacaact atctgaccca taagtatttt atttattttt
    11641 caatatgtta aacattatca gcgacaacaa taagctacga taacccactc tttttttgcc
    11701 ccttttttgc cccttctaca gcgttttgcc ccatttttgc catcaacaat tttcctcata
    11761 aagtcatttc aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag
    11821 gaaacaacct aatccctgtt ttaggcctca aagctgttaa tgataatcat tttcattttg
    11881 tatgggttat gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag
    11941 gtactaaatt atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac
    12001 ctgctcgatc gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac
    12061 catcatcgcg gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac
    12121 caagcctggt gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca
    12181 gggggcacaa ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta
    12241 ccagcatctc aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat
    12301 caccgaagga ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc
    12361 tctgatttac gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca
    12421 aacctaccaa cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct
    12481 gcgtaagcag attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc
    12541 tttttcttat ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc
    12601 cgatcaacaa ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca
    12661 tatcccggca gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg
    12721 tgaaaccggc gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg
    12781 cccggtacct acgtatatcg accttcttaa ggttactacc agtacgctgg tacagggaat
    12841 taaagccggg aaaagggaga aataatgatg caatctgcag gcattgtcgt taatgatgtt
    12901 actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac agtaccaagt
    12961 ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg
    13021 attatgggat ttgtgcgtct gaccagcgga aagatatctg ttctgggtat tcccacgcga
    13081 caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt tgactggtca
    13141 tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat gggtatgttg
    13201 cgtatagcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg
    13261 gtggattttc gccatcgtca aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt
    13321 ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc ttttaccggc
    13381 gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg cgcggaaggc
    13441 aaaacgatgc ttgtctcaac gcacaatctc ggctcagtca cgacattttg cgattatacg
    13501 gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt tacagccgag
    13561 aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacga ctcggaagaa
    13621 agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc ggtgcagagg
    13681 gaagcaagat gaacgtgcta ctggaaccct tcagctatga atatatgctc aatgcgatgt
    13741 gggtctcggc aatggtcggc ggtctctgcg ctttcctgtc atgctatctg atgctaaaag
    13801 gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt gcgggggcat
    13861 atatgctggg gcttccgttt tctctgggag ccttcttttc cggcggactg gcagcgggca
    13921 gcatgctgtt tcttaaccaa cgcacccgct taaaagaaga tgccatcatc ggcctgatct
    13981 tctcgtcctt ttttggcctg gggctgttta tggtgtcatt gaacccgaca tccgtgaaca
    14041 ttcagaccat cgtcctgggc aatattctgg caatcgatcc ggcggatatc ctgcaactca
    14101 ctatcattgg cattctctcg atcatcgtgc tttttttcaa atggaaagat cttatggtga
    14161 cctttttcga cgagaatcac gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc
    14221 tcttcttcac cctgctgtcg gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc
    14281 tggtgatctg tctggtggtg actcccggtg caacagcatg gttgttaacc gatcgttttc
    14341 cccgcctgct cataattgct gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg
    14401 tcagttactt tctggacggc gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt
    14461 ttcttttagc atttgtattt gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc
    14521 ataaagcgct ggaggatcgc tcatgatggc attattactg gagccactgc agttcacctt
    14581 tatgagtcac gcactgctga tttcactggt agtttccata ccctgtgcgc tgctgtcggt
    14641 ctttctggtc ctgaaaggtt gggcactgat gggtgacgcg atgagtcatg cggtttttcc
    14701 tggcatcgtg cttacctgga ttctggggtt acctctggct acaggggcat tcgtcgccgg
    14761 agtattctgc gcggtggcga cgggatacct gaaagacaac agccgaatca agcaagatac
    14821 agttatgggg attgtttttt ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat
    14881 caaaccagac gtacatcttg accatattct tttcggcgac atgctcggga taaccatcgg
    14941 cgatataatc cagacggtga ttattgccgg gctggttaca cttgttatta gcgcaaaatg
    15001 gcgggatttt ttgctgttca gctttgatta tcaacaggcg caggtaagcg gtttgcatac
    15061 gagatggctg cattatgggc tgctgtgcat ggtttccctg accattgtgg cgacgctgaa
    15121 agcagtaggt atcattctct ctatttctct gctcattgcg cctggcgcca ttgcagtact
    15181 cctgacacaa cgctttcata ttgcgctcct actggcgact ggcatatcgg taatagtgtc
    15241 aatgactggt gtctggctgt ccttttttat cgacagcgca ccagctccga cgattgtcgt
    15301 cttattcgcg gtcgtgttca tcatgacgtt taccgttacc agcatcaacg cacgcacaaa
    15361 gggaaacgcc catacacagg atctgttatc acccaattag cacaacatcc gaggacagca
    15421 tcgtccgcca gcctacagaa gtccggaaag ccatggaagg tcgttgggag gaggtacgaa
    15481 ttctatcatg caaaaaatac gtaaaatcga taacgcctag aaatcattca atactcgcac
    15541 tatcgaaagt ttaccagcca accgcggcac gttcctgcat acgacgtgcc gcagcttctg
    15601 ttattccacg ataaaattcc taaccgaatt caaatccttc agactattca gctcttcctt
    15661 cagctcccgc tgacggcgat aaatctcgtc attgcgctca acctgtgcct gagccattgc
    15721 tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat cggcatcacc
    15781 ccacgtcatc acgtcccgta caatgtcgga tttcgcagcc attaccaccg gataaaggcg
    15841 gcccagtgag ttggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt
    15901 cgcctcctgt tctgtgcgcc atgcttcaat t
//
LOCUS       DAZRVY010000092        15882 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZRVY010000092 DAZRVY010000000
VERSION     DAZRVY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.092"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..1453
                     /locus_tag="ACHXOX_002951"
                     /pseudo
     CDS             1..1453
                     /locus_tag="ACHXOX_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="ACHXOX_002952"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="ACHXOX_002952"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR7006359.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="ACHXOX_002953"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="ACHXOX_002953"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR7006360.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="ACHXOX_002954"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="ACHXOX_002954"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR7006361.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="ACHXOX_002955"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="ACHXOX_002955"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR7006362.1"
                     /translation="MTSLVSLKNVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="ACHXOX_002956"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="ACHXOX_002956"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR7006363.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="ACHXOX_002957"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="ACHXOX_002957"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR7006364.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="ACHXOX_002958"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="ACHXOX_002958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR7006365.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="ACHXOX_002959"
     CDS             complement(8434..8499)
                     /gene="yobI"
                     /locus_tag="ACHXOX_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFR7006366.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8573..9175
                     /locus_tag="ACHXOX_002960"
     CDS             8573..9175
                     /locus_tag="ACHXOX_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR7006367.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9177..9698)
                     /gene="ruvC"
                     /locus_tag="ACHXOX_002961"
     CDS             complement(9177..9698)
                     /gene="ruvC"
                     /locus_tag="ACHXOX_002961"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR7006368.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9733..10473)
                     /locus_tag="ACHXOX_002962"
     CDS             complement(9733..10473)
                     /locus_tag="ACHXOX_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7006369.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10502..10954)
                     /gene="nudB"
                     /locus_tag="ACHXOX_002963"
     CDS             complement(10502..10954)
                     /gene="nudB"
                     /locus_tag="ACHXOX_002963"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR7006370.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11072..12844)
                     /gene="aspS"
                     /locus_tag="ACHXOX_002964"
     CDS             complement(11072..12844)
                     /gene="aspS"
                     /locus_tag="ACHXOX_002964"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR7006371.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13154..13720
                     /locus_tag="ACHXOX_002965"
     CDS             13154..13720
                     /locus_tag="ACHXOX_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR7006372.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            13717..13812
                     /locus_tag="ACHXOX_002966"
                     /pseudo
     CDS             13717..13812
                     /locus_tag="ACHXOX_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14075..14323
                     /locus_tag="ACHXOX_002967"
     CDS             14075..14323
                     /locus_tag="ACHXOX_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR7006373.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14924..>15882
                     /locus_tag="ACHXOX_002968"
     CDS             14924..>15882
                     /locus_tag="ACHXOX_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7006374.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT         4038 a         4030 c         3642 g         4172 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtgtag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt gaaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaaat
     8521 aaatttttta tgctattacg tatattcgta tctatttcaa tggaatgaca acgtgaatat
     8581 taattaccct gctgaatatg aaattggaga tatcgtcttt acatgtatag gtgctgcctt
     8641 atttggtcaa atatcagctg catcaaattg ctggagtaat cacgtcggga tcattatcgg
     8701 tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctttcaa ccatcaccac
     8761 gctatctcgt tttattaaac gttcgtctaa tcaacgctat gctataaagc gattagacgc
     8821 tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc ggctacgcaa
     8881 actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa aatttgtttt
     8941 tgatatttat aaagaagcgc tatgcattcc ggtgggtgaa atagaaacgt ttggagaact
     9001 gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttct taggttctat
     9061 tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg gtctggtgtt
     9121 gattcacgcg gagggagttg aaacgcctca gcctgaactg accgaggcgg tataacttaa
     9181 cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcattgc attctggctg
     9241 acgtgacaat gggtaatcgc gatagctaac gcatcggcgg catccgcctg tggattagcg
     9301 ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc actaccaata
     9361 cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa ataccggcaa ttcctgattc
     9421 accgccgcca caatcgccac gccgcgcgcc tgccccagtt tcagagccga gtcggcattt
     9481 ttggccataa agacttgttc aatggcgaaa taatctggct ggaactgggt gatgatttcc
     9541 gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt ggtgcgtatg
     9601 catccgctac ccaggtagga cagttgcctg cctacctggc ggatgacacc atagccgatc
     9661 acgcgcgaac ccgggtcaat gccgagaata atagccatca cgcgtctccg ttttgctgtt
     9721 tagcaggccc catcagagag ttgctgcaac ctcatcagag atttcaccgt tatggtagac
     9781 ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt tcggtgcggt
     9841 ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc tgtctgcttt
     9901 cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg cagtgtagac
     9961 gtcaatcgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca gtgctgcttc
    10021 cataatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc tgaacagata
    10081 ggcaacggaa ccatcagtac cgaggttacc gccacatttg ctaaatgcat gacgcacttc
    10141 agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg tgccgccagg
    10201 accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc ccacaccacg
    10261 tgcaattgcg cggttcagtg tgtcacgggt catgttgtta gacagtgctt tatccaccgc
    10321 cgcacgcaga cgcgggttag cgtccggatc gccaccgccc agcttagccg cggttaccag
    10381 ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag ctttacgatg
    10441 tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata gccctgcctg
    10501 ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag gacttagtga
    10561 gcgccgcggc agcaggcgca tcaagccact tgtaagccag atgttcagtg aagactacct
    10621 ggcgttcgtg cgggagcgca agacagaacc atgattccgt attacgcgtc acgcccggcg
    10681 catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga cagtcaatta
    10741 aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg cgcatggcag
    10801 cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag aaatcgggat
    10861 cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcatagatg accactaaga
    10921 tcgaaacggg acgcttatac actttatcct tcacgctgcc tcttcgttga ctgccttcgc
    10981 tcatcccatt cacatagtta tctatgctca tgggagttca ctcagttgcc gccttgatgc
    11041 aacgcgaatg atttcgtgta tttgagtcat atcagttatt ctcagccttc ttcacaacct
    11101 gaatgctcag ctcagccagt acagtcgggt tagcaaagct cggtgcttca gtcatcagac
    11161 acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg gtgccagtca
    11221 gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga gtaccgtatt
    11281 tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg atacccagaa
    11341 taccaaacac cgtctgctgc atatcaccat tatggatacg tactgaacca ccgcccactt
    11401 cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt gcagctttca
    11461 gttctgcagc agtcatgtct ttcggtgagg tgaacggatg gtgcattgcc gtcaggccgc
    11521 cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc catttgcttt
    11581 cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcgcccatc gcgtcggcaa
    11641 caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg gcagtacgat
    11701 ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg ttgatacctt
    11761 ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg ccgtagattt
    11821 taacgaagtt accgtattcg tcgatctgct tacgggtcag cgatgcgcca cccggaacac
    11881 gtagagcggc aacacggcct ttcggatcgt tcgccggccc cgcaaatact gcaaactcaa
    11941 cagatttcag cagatcggca acgtcggtca gttccatcgg gttacgcaga tccggtttat
    12001 cagaaccata acggcgttct gcttccgcaa aggtcattac cgggaaatcg cccagatcca
    12061 cgcccttcac ttccagccac aaatgacgca ccagcgcttc catcacttca cgcacttgcg
    12121 gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga cgatcagcgc
    12181 gcaggtcttc gtcacggaag catttaacga tctgatagta acggtcaaaa ccggacatca
    12241 tcagcagctg tttgaacaac tgtggggatt gcggcagtgc gtagaattta cctttgtgca
    12301 cacgagaagg caccaagtag tcacgcgcgc cttccggcgt ggctttggtc agcatcggag
    12361 tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg gtgattttag
    12421 cgcgggtttt caggcgctgc gccatttccg ggcgacgcag gtcgaggtag cggtatttca
    12481 gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct gcgcggttga
    12541 tgatagtcag cgaggacgcc agcacttcaa tttcgccggt cgccatatcg cggttaatat
    12601 ttttttcgtc acgcgcacgt acggtgcccg taacctgaat gcagaactca ttacgcagtt
    12661 cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc acgatacctt
    12721 cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg ttgacccaac
    12781 cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa tattctgtac
    12841 gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca ggtgaccaag
    12901 tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat actggaaatt
    12961 ctgccgcacc gtaagagcct ggcccgcgct gtaacgcctc gttaccactt tatatcgggc
    13021 ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca taaaaattaa
    13081 caaaatttgt cgttccgcca tcggctaatc gcattaaggt gtgaggcacg attttgtttt
    13141 ttcaggagtc agcatgcttg aacttaatgc taaaaccact gcgttggtgg tgatcgattt
    13201 acaagaaggc atcttacctt ttgccggtgg tccacatact gccgatgagg tagttaatcg
    13261 cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc tggtgcgagt
    13321 tggctggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc cctcccccgc
    13381 taaagtgttg cccgaaaact ggtggcaaca tccagctgca ttaggtgcaa ctgacagtga
    13441 tatcgaaatc atcaaacgtc agtggggtgc gttttacggt acggatctgg agttgcaact
    13501 gcgccgccga ggtatcgata caatagtgtt atgtgggatc tcgaccaata tcggtgttga
    13561 atccaccgcc cgcaatgcct gggaacttgg ctttaatctg gtgattgccg aagacgcctg
    13621 tagtgccgcc agcgccgagc aacacaataa cagcattaat catatctacc cgcgcatcgc
    13681 ccgtgtgcgt agcgtggaag agatccttca cgcgttatga tttacatcgg tctaccgcaa
    13741 tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta tgcccgccac
    13801 tttaactgcg tgatacggga tttttaaaaa tcactaaaga acgcccaaga gcatgtgttt
    13861 tctttagaat aatcaaagcg ttggattgcc atgccatatg aaaatcggca aagttcatat
    13921 gtaacaaagt gtcccaatca tgccccaaaa tgccccacaa caaatatttt tgccccatgc
    13981 atgccccaaa aaatcacttt ccctcactct tgacactgtt tatccataca gttaaaaata
    14041 atactgtata aaaacacagt gtagagggat ttttatgcgt attgaaatct gcatagccaa
    14101 agagaaaatg actaaaatgc caaccggtgc tgtggatgcg ttaaaggaag aattaacccg
    14161 acgcatcagt aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg
    14221 cctttctgtt acgcgcaccg ccgataaaga ttcagctaaa acttttgttc aggaaactct
    14281 gaaagatacc tgggagtctg ctgacgagtg gtttgttcac taattagcac gtaaaatcag
    14341 taacggctgg aaatcattca atactcgcac tatcggaagt tcaccagcca gccgcagcac
    14401 gttcttgcat acgacgtgtc tgcggtctca actccgcccg cccccgccaa tcatgattgg
    14461 gcagtaccag tacagcacta gcaaagacag gaaattagca gtctcagcaa aacaccgacc
    14521 agacggtgag aagacaaaaa gatacgcaaa gatgccgcgg ttccagaaga ataaaaaacc
    14581 gctaatggac agcctcctta acacagtttt ttatatacat gattcactga taccagtggc
    14641 attaatagct attatatagc ttcagctcct tttttcatac cttaaggaaa aattgccccc
    14701 tcccccaaaa agtatcgttt ttttacatta aaccgatcca gtttaggata gtgtaaagca
    14761 gaagttcttc aatatataac atatggagtt aatatgctta cacaaactat atatccgcct
    14821 cctccagaag ttcctgtcta ctgctgaagg gaaccaataa cattgcaaag cagataaaaa
    14881 agaatccaac acctctcaac ataaagaata cgccctgata tttatgctcc cgacaaataa
    14941 caatcacaga ttaatttcaa attcgttctc cacttattca atcgacacta gccgtgcata
    15001 tgaaaattat ctaacccatt ggactgaatg gaaaaataac cgcatacaag aagaacaacg
    15061 agacatcgct tttcagcgac tagtatcatg tctacaaaac caagagacga acctggactt
    15121 gtctgaatta ggcctgacaa cattacctga aatccccccg ggaattaaat caattaatat
    15181 aagtaaaaat aatttaagct taatctcccc attgcctgcg tcccttacac agcttaatgt
    15241 cagctataac agacttattg aactgcctgc tttgcctcaa ggacttaaat tattgaatgc
    15301 gtcccacaat caactaatca cactacccac actccccata tctttgaagg agcttcatgt
    15361 ctcaaataat caattatgtt ctcttcctgt tttaccagaa ctactggaaa cattagatgt
    15421 atcatgtaat gggctggcag ttttaccacc tttaccattt tctttacaag agattagcgc
    15481 aatagggaat cttcttagtg aactcccccc tctacctcac aacattcact ccatatgggc
    15541 aatcgacaat atgttaaccg atattccata cctgccggaa aatttaagga acggttattt
    15601 tgacataaat cagataagtc atatcccgga aagcattctt aatctgagga atgaatgttc
    15661 aatagatatt agtgataacc cattgtcatc ccatgctctg caatccctgc aaagattaac
    15721 ctcttcgccg gactaccacg gcccgcagat ttacttctcc atgagtgacg gacaacagaa
    15781 tacactccat cgccccctgg ctgatgccgt gacagcatgg ttcccggaaa acaaacaatc
    15841 tgatgtatca cagatatggc atgcttttga acatgaagag ca
//
LOCUS       DAZRVY010000093        15511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZRVY010000093 DAZRVY010000000
VERSION     DAZRVY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.093"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHXOX_002969"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHXOX_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR7006375.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHXOX_002970"
     CDS             567..650
                     /locus_tag="ACHXOX_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006376.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHXOX_002971"
     CDS             750..1571
                     /locus_tag="ACHXOX_002971"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR7006377.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXOX_002972"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXOX_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR7006378.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXOX_002973"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXOX_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR7006379.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXOX_002974"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXOX_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR7006380.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXOX_002975"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXOX_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR7006381.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXOX_002976"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXOX_002976"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR7006382.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXOX_002977"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXOX_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR7006383.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHXOX_002978"
     CDS             8347..9729
                     /locus_tag="ACHXOX_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7006384.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHXOX_002979"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHXOX_002979"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR7006385.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHXOX_002980"
     CDS             complement(10485..10820)
                     /locus_tag="ACHXOX_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR7006386.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXOX_002981"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXOX_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR7006387.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXOX_002982"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXOX_002982"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR7006388.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHXOX_002983"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHXOX_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR7006389.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXOX_002984"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXOX_002984"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR7006390.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3794 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZRVY010000094        15406 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZRVY010000094 DAZRVY010000000
VERSION     DAZRVY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.094"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHXOX_002985"
     CDS             <1..453
                     /locus_tag="ACHXOX_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7006391.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHXOX_002986"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHXOX_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHXOX_002987"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHXOX_002987"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR7006392.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHXOX_002988"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHXOX_002988"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR7006393.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHXOX_002989"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHXOX_002989"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7006394.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHXOX_002990"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHXOX_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR7006395.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHXOX_002991"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHXOX_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR7006396.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHXOX_002992"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHXOX_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR7006397.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHXOX_002993"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHXOX_002993"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR7006398.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHXOX_002994"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHXOX_002994"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR7006399.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHXOX_002995"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHXOX_002995"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR7006400.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHXOX_002996"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHXOX_002996"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR7006401.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHXOX_002997"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHXOX_002997"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR7006402.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHXOX_002998"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHXOX_002998"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR7006403.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHXOX_002999"
     CDS             13055..13435
                     /locus_tag="ACHXOX_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR7006404.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHXOX_003000"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHXOX_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR7006405.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHXOX_003001"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHXOX_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR7006406.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHXOX_003002"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHXOX_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR7006407.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4042 c         3851 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gcactggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZRVY010000095        15170 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZRVY010000095 DAZRVY010000000
VERSION     DAZRVY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.095"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXOX_003003"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXOX_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR7006408.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXOX_003004"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXOX_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR7006409.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXOX_003005"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXOX_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR7006410.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXOX_003006"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXOX_003006"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR7006411.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXOX_003007"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXOX_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR7006412.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHXOX_003008"
     CDS             complement(8214..9107)
                     /locus_tag="ACHXOX_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR7006413.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXOX_003009"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXOX_003009"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR7006414.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXOX_003010"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXOX_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR7006415.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXOX_003011"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXOX_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR7006416.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXOX_003012"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXOX_003012"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR7006417.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXOX_003013"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXOX_003013"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR7006418.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15170)
                     /locus_tag="ACHXOX_003014"
     CDS             complement(14275..>15170)
                     /locus_tag="ACHXOX_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR7006419.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVSHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3740 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgagatacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg
//
LOCUS       DAZRVY010000096        15143 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZRVY010000096 DAZRVY010000000
VERSION     DAZRVY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.096"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHXOX_003015"
     CDS             <1..122
                     /locus_tag="ACHXOX_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7006420.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHXOX_003016"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHXOX_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR7006421.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="ACHXOX_003017"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="ACHXOX_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR7006422.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHXOX_003018"
     CDS             3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHXOX_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR7006423.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            4417..6099
                     /locus_tag="ACHXOX_003019"
     CDS             4417..6099
                     /locus_tag="ACHXOX_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR7006424.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6151..7308
                     /locus_tag="ACHXOX_003020"
     CDS             6151..7308
                     /locus_tag="ACHXOX_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR7006425.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7274..7396)
                     /locus_tag="ACHXOX_003021"
     CDS             complement(7274..7396)
                     /locus_tag="ACHXOX_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006426.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            7605..9290
                     /locus_tag="ACHXOX_003022"
     CDS             7605..9290
                     /locus_tag="ACHXOX_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR7006427.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            9328..11700
                     /locus_tag="ACHXOX_003023"
     CDS             9328..11700
                     /locus_tag="ACHXOX_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR7006428.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11757..14540
                     /locus_tag="ACHXOX_003024"
     CDS             11757..14540
                     /locus_tag="ACHXOX_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR7006429.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            14902..>15143
                     /locus_tag="ACHXOX_003025"
     CDS             14902..>15143
                     /locus_tag="ACHXOX_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7006430.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4446 a         3297 c         3289 g         4111 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa actttattgt
     3241 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
     3301 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
     3361 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga tttactgaaa
     3421 ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg cctgttcctg
     3481 cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc
     3541 ttaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
     3601 ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
     3661 ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
     3721 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
     3781 cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
     3841 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
     3901 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
     3961 gtcaaagtca catgggtatg aatacgggca acacgatgag tgctttagct atttgattat
     4021 ttgctaacga gtagtcaacc acgcacgctg cgtaagaatt aaatggggca gccattccct
     4081 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtgacat ccatcacata
     4141 tttatgcact tgcataacct gttgcagatt atttatgatc tcaattcggc attttgtcag
     4201 taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga gaaattactt
     4261 aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt tttagctaaa
     4321 tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct tcgtaaaatt
     4381 acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt aaagaaaagt
     4441 gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt tgctgctcat
     4501 gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc agattttacg
     4561 ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat ggatgatctt
     4621 ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat ggaaaatcgt
     4681 gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc acaaaaatca
     4741 acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg ctatgtggca
     4801 cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt
     4861 ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac tttcttgcct
     4921 gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca cttgtcaaaa
     4981 atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga caacttcacc
     5041 acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt tatgggattc
     5101 cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc
     5161 cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt tgttgatcgt
     5221 gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc gcacctacca
     5281 aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag tcaaacagca
     5341 gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat tctcgaacaa
     5401 ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga taatggtgcg
     5461 gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag tcagacctat
     5521 cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca acccggtaat
     5581 tatgacaagc tgatttccgc aatggatttc tacccaacag ctcttgatgc agccgatatc
     5641 agcattccaa aagaccttaa gctggatggt gtttccttgc tgccctggtt gcaagataag
     5701 aaacaaggcg cgccacataa aaatctgacc tggataacct cttattctca ctggtttgat
     5761 gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca gtcagacgat
     5821 tacccgcata accccaacac tgaggactta agccaattct cttatacggt gagaaataac
     5881 gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa actgacggat
     5941 ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga gatgcaaggc
     6001 gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa tcaggagaag
     6061 tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca tgcggcggat
     6121 ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc ctccagtttt
     6181 caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca gtttactcat
     6241 gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat cgaagcgtct
     6301 ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg cctggatttt
     6361 ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat ttttaatgca
     6421 ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa agaacatgaa
     6481 tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta cagacgcagt
     6541 aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct gaaatcatat
     6601 caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta ccctcttgag
     6661 gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga attgctagaa
     6721 accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg tatcattgat
     6781 ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat gcaatgggtt
     6841 aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag ccgttttttg
     6901 gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct ggttgttgaa
     6961 agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa aattggaaac
     7021 attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca aaaaaaacgg
     7081 atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg ttgtcctaag
     7141 catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg ttataaaatc
     7201 cttttttcaa ccatggtacc ttatatgaac gccatggttg agttagctaa gaacagagta
     7261 ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata atgctctctt
     7321 ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca taaattcctg
     7381 tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt ggtatgcgtc
     7441 agggcattag caataccctt gattatcaat agcataacca taaccataac cactgaaaat
     7501 taaaaatttc tatatatttc atattgttaa atgatactta aataaatttc atcagtaaat
     7561 cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata accattccca
     7621 ttacgtttta tatgttgata gcaaagtatc tctgtctgct gaagcccttt tggctgcgta
     7681 agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc cttggcgtgg
     7741 ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg ctaagccaga
     7801 aagaaaccga taagctttgg cagcttgttc tttggttccc tgccctgctg gggatctttg
     7861 tattaatatc cgtaaacaaa acatggttaa ttaaactact gactattcgc tggcgcgaat
     7921 ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac ttcacgcaga
     7981 tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa gacattctct
     8041 tactgatcag caaaacgctc agtctttcat ttggcttcat ccagtcactt agcatgctga
     8101 tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact gttggtggaa
     8161 cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg atcggtggaa
     8221 ctttatttac ccataaggtg ggtaaacgta ttcgtccgct taatgtggaa aaacaacgta
     8281 gcgaagcgac atttcgaact aatcttgtgc agcataataa gcagacagaa ctgattgcgc
     8341 taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat accattaaag
     8401 agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa aatatttatt
     8461 cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt agcgggcaga
     8521 tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg aacaatttaa
     8581 gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc gatcgcttgt
     8641 atgagttcca tcaactcact gaacagcgcc ctacgaataa gcctaaaaat tgccaacatg
     8701 cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata ttagagaact
     8761 tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct ggtgcgggaa
     8821 aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt gatatttctt
     8881 ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaagcggc ttactgaaag
     8941 agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc gaagtactgc
     9001 atcaggttgg tctggggaaa ttggctgcgc gtattcatga ccacgatcgc tggggagata
     9061 ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta cgacgtccga
     9121 aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct atacgcttac
     9181 tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca catcaacccg
     9241 gtgtctggaa cctggctgat gatatttgtg acattagcac ggttttataa caacaataaa
     9301 agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg cgtcatttta
     9361 tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca tttttttgaa
     9421 gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca ttacaccagt
     9481 gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct gagaaccaac
     9541 cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga tattcgccct
     9601 gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat tgacggtatt
     9661 agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc aaccaatatt
     9721 agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt gacgctttat
     9781 gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga tgcaaagatc
     9841 aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac gcgttcggac
     9901 tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg ctcttcagga
     9961 agtacttatt actccccaga ttttaaaaag aacttttata ccttgtcgtt taatcaggaa
    10021 ctcgctgata acttcggcgt taccgccggt ttatcgcgcc gccagtctga tatcacccgc
    10081 gcggattatg tttcgaatga cggcattgtc accggtcggg cacagtataa aaacgttatc
    10141 gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca cgatttaacc
    10201 ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca gtctgatcgc
    10261 gaaatgggta ataaatccta tggtctggca tgggatatgg atacgcaact cgcatgggcc
    10321 aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca cgatcatgac
    10381 atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg tacctgtggc
    10441 ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg cctggactgg
    10501 caaaaattcg ccgtgggtga tgtttcgcat caaccctact tcggcgcgga atacatctat
    10561 tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa tgctgcagga
    10621 aagaaaacta accataccat ttaccataaa ggtaaaggca gcctgggaat tgacaactac
    10681 acgctgtata tggcggagca cattagctgg cggaatgtgt cgttaatgcc cggtgtgcgt
    10741 tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat gacggaatgg
    10801 gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta ctatggcggg
    10861 aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc ggtatcaggt
    10921 aataaaactc tgacgcgtta tcaggatttg aaaacgcctt ataacgatga actggcaatg
    10981 ggattgcagc agaaaatcgg taagaacgtt attgcgcgtg caaactatgt ttaccgtgaa
    11041 gcgcatgatc aaatcagcaa aagcagtcgt accgacagcg cgactaaaac caccattact
    11101 gaatataaca acgatggcaa aaccaaaacg cattcgttca gcctcagttt tgaactggcc
    11161 gaacccctgc atatccgcca agtagatatt aacccacaaa ttgtctttag ctatatcaag
    11221 agcaagggca acttgtcgtt aaacaatggt tatgaggaga gcaataccgg tgataaccag
    11281 gtggtttata acggtaatct ggtctcttac gatagtgttc cagtggcaga ttttaataac
    11341 ccattaaaga tctccttaaa catggatttc acgcatcaac cgagcgggtt agtgtgggcg
    11401 aatacgctgg cctggcaaga agcgcgtaaa gctcgcatta tccttggtaa gacaaatgcg
    11461 caatacatca gcgaatattc agattacaag cagtatgttg acgaaaaact ggatagcagc
    11521 ctgacctggg acacccgctt gtcctggacg ccacaatttc tgaaacaaca aaacctgacg
    11581 atcagtgccg atattctcaa tgtactggat agcaaaaccg ctgttgatac aacgaacacc
    11641 ggtgtggcga cctacgccag tggccgtact ttctggcttg atgtcagcat gaaattttaa
    11701 tagttcggat tttacttaac cgggtaaatt cgcccggttt tcgcatggag ataattatga
    11761 gaaacctctg tttcttactg acgttagtgg caactctgtt gctccccggg cggctgattg
    11821 ccgccgcctt accgcaggat gaaaagttaa ttaccgggca actggacaat ggcttgcgat
    11881 atatgattta tccgcatgct caaccaaagg atcaggtaaa tttatggctg caaattcata
    11941 ccggttcatt gcaggaagaa gacaatgagc gcggcgtggc tcattttgta gaacatatga
    12001 tgtttaacgg cacaaaaaca tggccgggta ataaagtcat cgaaacattt gagtcaatgg
    12061 gcctgcgttt cggtcgcgat gttaatgcct ataccagcta tgacgaaacg ctgtatcagg
    12121 tgagtctgcc gactacgcag aaacaaaatc tgcaacaagt gatggcaatc ttcagtgaat
    12181 ggagtaatgc cgcaaccttt gaaatactcg aagtagacgc tgaacgcggc gtaattactg
    12241 aggaatggcg tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc cgccctttcc
    12301 tgctggcaaa tacccgtaat ttagaccgtg aacctatcgg cctgatggat actgtcgcca
    12361 cggtcacacc ggcacaattg cgccaatttt atcaacgctg gtatcaacca aataatatga
    12421 cctttatcgt ggtcggcgat atcgacagta aagaagcgct ggcgctgata aaggataatt
    12481 taagtaagct tccggctaac aaagcagctg aaaatcgcgt ctggccgaca aaagccgaaa
    12541 accacctgcg ctttaatatc attaatgata aagaaaaccg agtgaacggc atcgcactct
    12601 attatcgcct gccaatggta caagtgaacg atgagcaaag ctttgtcgaa caagctgaat
    12661 ggagcatgtt agttcagctg ttcaatcaac gtctgcagga acgcatacag tcgggcgagt
    12721 tgaagactat ttctggcggc actgcgcgca gcgttaaaat tgcacccgat tatcagtcgc
    12781 tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcaaat gcattaatgg
    12841 cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat gatgtcaaat
    12901 ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt gatttacgta
    12961 tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg tcgccggaag
    13021 agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt ctggcggaaa
    13081 aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta aacaatgagc
    13141 ttgccgccaa aaaagcattg tctcttgcgg ctatcctggc gctggaaaaa gagtacgcca
    13201 acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca gtagacgctg
    13261 acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca tcattaacac
    13321 tttccaatgg tgccagggtt attctggcaa aatccgcggg tgaagagcaa aagctacaaa
    13381 ttactgccgt atctaataaa ggcgatttaa gtttccctgc gcagcaaaaa tcacttatcg
    13441 cgctggcaaa taaagcagta agcggaagcg gcgttggtga gctctcctct tccagcctga
    13501 aacgctggag tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc atgaatacgt
    13561 tgctctccgt tagcgcgcgg actaataacc ctgaacctgg ttttcagttg attaaccagc
    13621 gaatcaccca cagcacgatt aacgacaata tttgggcatc gctacaaaat gctcaaattc
    13681 aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagcag atgtatgaga
    13741 cgcgctatgc tgatgaccgc acgaaattac tgcaagaaaa tcagattgta cagtttactg
    13801 ccgcagatgc gctggctgcc gatcgccagt tgttttcatc tccagcggat atcacgtttg
    13861 tcattgtcgg taatgtctca gaagacaaac tcgtggcgtt aattacgcgt tacttaggat
    13921 caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc gcgacggaca
    13981 acgcatcggt tactgtaaaa gaacaaaatg aacctgtggc acaggtttca cagtggaagc
    14041 gttatgattc ccggacacct gttaatctgg cgacgcgtat ggcgctcgat gcttttaacg
    14101 tcgcactggc aaaagatcta cgcgttaata ttcgtgaaca ggcatctgga gcatacagcg
    14161 tttcttctcg cctctcggtt gatcctcagg ccaaagatat cagtcatttg ctggctttta
    14221 cttgtcaacc agaacgacat gatgaactgt taacgttagc gaatgaagtg atggttaagc
    14281 gtctggctaa agggatcagt gagcaagaac tgaatgaata ccagcaaaac gttcagcgca
    14341 gcctcgatat ccaacagcgt agcgttcaac aattagcgaa cactattgta aatagtctta
    14401 ttcaatatga cgatcctgca gcatggactg agcaggagca attgttgaaa caaatgacgg
    14461 tagagaatgt taacactgcc gttaaacaat atctttctca tccggtaaat acttatactg
    14521 gagtattatt gccaaaataa taacagcccc gtcaacacat cgttggcggg gattttagca
    14581 atattcgcca tttttatgta ataattttat aaatgcgttc aaaataataa ccaggtacta
    14641 atagtgatat tttaaggtct gatttttacg tgaaaattca ggaggcacag aatgcgcata
    14701 aaaataacag cataaaacac cttaccacca cccaagaatt tcatattata ttgtttttca
    14761 atgaaaaaat attattctcg taatatctca cgataaataa cattaggatt ttgttattta
    14821 aacacgagtc ctttgcactt gcttacttta tcgataaatc ctactttttt aatgcgatcc
    14881 aatcatttta aggagtttaa aatggataag aagcaagtaa cggatttaag gtcggaacta
    14941 ctcgattcac gttttggtgc gaagtctatt tccactatcg cagaatcaaa acgttttccg
    15001 ctgcacgaaa tgcgcgacga tgtcgcattc cagattatca atgacgaatt atatcttgat
    15061 ggcaacgctc gtcagaacct ggccactttc tgccagacct gggacgacga aaatgtccac
    15121 aaattgatgg atttatccat taa
//
LOCUS       DAZRVY010000097        15029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZRVY010000097 DAZRVY010000000
VERSION     DAZRVY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.097"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHXOX_003026"
                     /pseudo
     CDS             complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHXOX_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            2145..2732
                     /gene="betI"
                     /locus_tag="ACHXOX_003027"
     CDS             2145..2732
                     /gene="betI"
                     /locus_tag="ACHXOX_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR7006431.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2746..4218
                     /gene="betB"
                     /locus_tag="ACHXOX_003028"
     CDS             2746..4218
                     /gene="betB"
                     /locus_tag="ACHXOX_003028"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR7006432.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4232..5902
                     /gene="betA"
                     /locus_tag="ACHXOX_003029"
     CDS             4232..5902
                     /gene="betA"
                     /locus_tag="ACHXOX_003029"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR7006433.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHXOX_003030"
     CDS             6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHXOX_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR7006434.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7026..7721)
                     /locus_tag="ACHXOX_003031"
     CDS             complement(7026..7721)
                     /locus_tag="ACHXOX_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR7006435.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7714..9141)
                     /locus_tag="ACHXOX_003032"
     CDS             complement(7714..9141)
                     /locus_tag="ACHXOX_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR7006436.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9152..9871)
                     /locus_tag="ACHXOX_003033"
     CDS             complement(9152..9871)
                     /locus_tag="ACHXOX_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR7006437.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="ACHXOX_003034"
     CDS             complement(10399..11253)
                     /gene="rclR"
                     /locus_tag="ACHXOX_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR7006438.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11479..12804
                     /gene="rclA"
                     /locus_tag="ACHXOX_003035"
     CDS             11479..12804
                     /gene="rclA"
                     /locus_tag="ACHXOX_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR7006439.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            12913..13149
                     /gene="rclB"
                     /locus_tag="ACHXOX_003036"
     CDS             12913..13149
                     /gene="rclB"
                     /locus_tag="ACHXOX_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR7006440.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13161..13754
                     /gene="rclC"
                     /locus_tag="ACHXOX_003037"
     CDS             13161..13754
                     /gene="rclC"
                     /locus_tag="ACHXOX_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR7006441.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(13914..14783)
                     /locus_tag="ACHXOX_003038"
     CDS             complement(13914..14783)
                     /locus_tag="ACHXOX_003038"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR7006442.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         3938 a         3828 c         3594 g         3669 t
ORIGIN      
        1 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg
       61 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      121 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      181 ttgccctggc tgccttctaa caggaaggtt tccagtcggt agtaggtcga tttaccgata
      241 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      301 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      361 ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      421 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      481 ttcatcaggc gcgatagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      541 ggcgcggtat cgcggttgac actttcacgg cggtaatctt ctaccttcag agatttatac
      601 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      661 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag accaatcgcc
      721 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      781 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      841 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      901 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
      961 tcatacagcg cgctattgcc gaataccgag agccataaca gcgtgaaggt aaacggaata
     1021 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1081 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1141 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1201 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1261 aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1321 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1381 gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1441 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1501 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1561 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1621 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1681 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1741 tcggctacgg agaagaacat caggtcgata ccgatcccgc cagcaaacag catcgccgcc
     1801 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1861 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag tagatagtac
     1921 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     1981 cgaaacagga tcgttgtcag ggaaaaggtc tgtcatccag tatccccact gttattgtta
     2041 ccatttaaaa tcaaattcgt aacaattacg acacatttta tattgaacgt ccaatcaata
     2101 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2161 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2221 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2281 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2341 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2401 agcgattaca ggcgattgtt gccggaaact tcgatgaaac tcaggtgagc agtgcggcga
     2461 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2521 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2581 cgcgcgaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2641 tgcgcgcggc actaagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2701 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2761 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     2821 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     2881 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     2941 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3001 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3061 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3121 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3181 gtggcgggga ttggcgcatg gaactacccg attcagattg ccctgtggaa atccgccccg
     3241 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3301 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3361 ccgggggtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3421 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3481 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgatgatgcg
     3541 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3601 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3661 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3721 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3781 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     3841 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     3901 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     3961 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4021 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4081 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4141 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4201 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4261 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4321 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4381 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4441 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4501 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4561 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4621 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4681 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4741 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4801 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     4861 gcgccctaac ctgaccattc gtactcacgc tatgaccgat cacatcattt ttgacggcaa
     4921 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     4981 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5041 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5101 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5161 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5221 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5281 cagccgagag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5341 ctataacggc tcgaatgcgg tgaaggagca cggtttccag tgccacgtcg gctcaatgcg
     5401 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5461 tctgtttaac tacatgtcac acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5521 cacccgggag atcatgcatc aaccgacgct ggatcagtat cgtggccgcg aaatcagccc
     5581 cggcacggaa tgtcagacgg atgagcagct cgatgagttc gtgcgtaatc acgccgaaac
     5641 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5701 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5761 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     5821 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     5881 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     5941 gagttaccgt tccagagagg gggaccgaat ccttatataa acactgaggt aactctcatg
     6001 cttcatatcc agtattcatg atgctggttt tttgctgata tttaccccga acattcctga
     6061 tacagactat taatacaacc ttatctgcgg cagattttat tttctggata tattatgagt
     6121 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaagatt
     6181 cgtgttatcc tttttattat agctatctgt atggcaatgg tgcttttatt tctgtatgtc
     6241 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6301 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6361 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6421 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6481 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6541 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6601 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6661 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6721 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6781 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6841 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     6901 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     6961 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7021 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7081 actttgataa gctcaatatc cgccgttgag ctgggaccac tgatgatgtt aatacatgac
     7141 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7201 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7261 cgcccgcgct cgacggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7321 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7381 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7441 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7501 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7561 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7621 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7681 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgcctg
     7741 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7801 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7861 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     7921 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     7981 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8041 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8101 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8161 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8221 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8281 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8341 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8401 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8461 cattcgcgca ttaccttcat tggtcactag gcataccgaa cctgtctctg ccaccgcgaa
     8521 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8581 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8641 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8701 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8761 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8821 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     8881 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     8941 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9001 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9061 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9121 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9181 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9241 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9301 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9361 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9421 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9481 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9541 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcgggt tcatccgcca
     9601 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9661 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9721 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9781 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9841 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
     9901 tatgcccgat ggttcccctc cttccggggg atcaatcatc aataaatatg gcgttctttt
     9961 ttaattggct agcaacatac cacccctcat ttgtgtgaca aatgataatc tttcatccaa
    10021 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
    10081 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    10141 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
    10201 caataatcac gtatcaagta atagtcgggg ttttatttaa ttgaagcaat ttagtttgca
    10261 tttttattta aaaactgagc tagagtccat cagaaaaaaa tatataaagc ccaaaccacc
    10321 gttggaaata gaaatagttg aaaggctcac aactcccgga atgcggatta tctttgccct
    10381 gatttctggc ggttttattc agggtgcaag ctgtctgacc ctttcccgat attctcccgg
    10441 agtacaacca aactcgcgga caaacgcctt gtgaaaagat gattcactgg catagcctac
    10501 tgactcagcg atcaccacaa cagggagcat ttcccgggaa aacatctggg ccgctatttg
    10561 tagacgcaac tttgttaata cagccagcgg cgtggttccg gaaacatcac ggaaaagctg
    10621 ggcaaaactt gcccgagaca tgtgggcgat gctggccagc gattcgacgg tccaggcgtg
    10681 tcctggcatt tccagcattt gctgtattac cgcaccaaga cgtggatgca gaagcaaact
    10741 gagaatgttt ttctctgtat taacctgtgc aatccactca cgcaccgcga gggtaaagaa
    10801 tgtagcgcag atctggctac acagtgcatc cactcccggc atcgccgatc tggattcctg
    10861 ttgtaaaaac gggatcgcct ccctcagcca gttatattcc acgctgtggt taaccggtgc
    10921 taaaaacagc gtttccggca aagacgttaa aaaataacgc gccgaatgtt gcaaccgaag
    10981 agtgccgcag acaatacagg tcgattcatt atcgacatga ctcaggcgat gagctgaatt
    11041 ttgtggtagc aggaccacat ttcccgggcg taatgtaaaa atctctcccg tcggcatttc
    11101 cagcttcgcc gctccttgcg ttaacgcatg ccaacgaata accgataatt ccccggcacc
    11161 atgttgaagc tgccagtcac ttcctaacac gcaattctta tcgatcgttc cttgtggagc
    11221 gttaagcatc aacaaacggc taagggcatc catatcagac tcctgagtgg tttttgatta
    11281 caaacaccaa caatcttagt aaataactaa tctaatacca tcaaatggtt aatcatgatt
    11341 tttgttttgt gattctgatt aagaaaaaga aaataccaga cgataagaca aaatatcaag
    11401 cgttgtgaag aaatggtatt tgctcttttg cgtctatagt catgatgtca aatgaacgtg
    11461 tttcgacagg aaatcatcat gaataaatat caggcagtta ttattggttt tggcaaggct
    11521 ggaaaaacat tagccgtcac gctggcaaaa gcaggttggc gagtggctct catcgaacaa
    11581 tcaaatgcaa tgtatggcgg gacctgtatt aatatcggct gcatcccaac caaaacattg
    11641 gttcatgacg cacagcagca cacagatttt gtccgtgcca tacagcgtaa aaatgaagtg
    11701 gttaattttt tacgtaataa gaattttcat aatcttgcgg atatgcccaa tatcgacgtg
    11761 atcgacggcc aggcggagtt tatcaataat catagcctgc gtgttcatcg gcctgaggga
    11821 aatctggaaa ttcatggcga gaaaattttt attaataccg gtgcacaaac cgtggttccg
    11881 ccaattcctg gaattaccac cacgcctggt gtatatgaca gcaccggatt acttaatcta
    11941 aaagaattgc ctgggcattt aggtattttg ggcggcggat atattggcgt tgagttcgcc
    12001 tctatgttcg ctaattttgg cagcaaggta accattttag aagcagcttc gctgtttttg
    12061 cctcgggaag atcgggatat tgctgataat atcgcgacga ttttacgcga tcagggcgtc
    12121 gatattatcc tcaatgccca tgtggagcga atcagtcacc atgaaaatca agtgcaagtg
    12181 catagcgagc acgcccaact ggcggtggat gcactgttaa tagcttccgg tcgtcaaccg
    12241 gctaccgctt cgttacatcc agaaaatgcc ggtatcgcag taaacgagcg cggggcaatt
    12301 gtcgttgaca agcaattaca taccaccgca gacaatattt gggcgatggg agatgttacc
    12361 ggcgggctgc aatttactta tatttcactg gatgattacc gcattgtgcg tgatgagttg
    12421 ctgggagaag gcagacgtag tactgatgat cggaaaaatg tgccttattc cgtattcatg
    12481 acaccgccac tgtccagggt tggtatgaca gaagaacagg ccagagagag tggtgctgat
    12541 attcaggtgg tgacattgcc tgtagctgca attccgcgtg ccagagtgat gaatgatact
    12601 cgtggggtat taaaagcgat tgttgataat aaaacccaac gtatattagg ggcatcactg
    12661 ctgtgtgttg actcccacga gatgatcaat atagtgaaaa tggtgatgga tgccgggctg
    12721 ccttatagca tattacgcga tcagatattt actcatccgt cgatgagcga atcactcaat
    12781 gatctatttt cattagtcaa ataaactcaa aatcagacgc cagaacaaat attctggcgt
    12841 ctcagagaaa agaatcttat taattctctg ttactctata acttcttaat cacttcattg
    12901 atggtatttt atatgtttaa aaaatctgtt ttatttgcaa cactattatc tggcgttatg
    12961 gaattttcca ccaatgcaga tgataaaata attctgaaac atatcagcgt ctcgtcagta
    13021 tcagcatcac cgacagttct ggaggatacc attgctgata tagccagaaa atataatgct
    13081 tcatcctgga aagtcacatc gatgcgaatt gataataatt caaccgcaac agcagtattg
    13141 tataaataag gatgttcaca atggaaaaat acctgcacct gttaagtcgg ggagataaaa
    13201 ttggcctgtc attgattcgt ctgagtattg cgattgtttt tatgtggatt gggttattaa
    13261 agtttgtccc ttacgaggca gacagcatta caccattcgt cgcaaacagt ccactaatgt
    13321 cgttctttta tgaacacccg gaagactata aacagtatct gactcacgaa ggcgaataca
    13381 aaccagaagc aagggcatgg caatcggcca ataataccta tggtttttcc aacggtcttg
    13441 gcgtcgtgga ggtgattatt gctctgctgg ttttggctaa tcctgtcaat cgctggttag
    13501 gtttattggg aggactgatg gcatatacca cgccgctggt aacactctca tttttaatca
    13561 ccaccccgga ggcatgggta cccgcattgg gtgacgctca tcatggtttc ccttatttat
    13621 ccggtgctgg tcgcttggta ttgaaagata ctctgatgct ggcaggtgct gtcatgataa
    13681 tggcagattc ggcgcgggaa attcttaaac aacgcagtaa tgaatccagt tcaacgttaa
    13741 aaactgaata ttgataatcc gaacactgtc tgttgttcac tttttcaggc gtcaggcccc
    13801 ctttctgatg atgaagcctg acgccatatt accggagata tgacagagtg aagatcaata
    13861 tcactcccct gttaaaagat tcttgtcgtc tcaggtaaaa tatatgatta atatcaatca
    13921 tgtattttta cagcaagaca accacgaaca aattcaggat taaaatgttt taccgactcc
    13981 ccaacataac ccaaatcaag cgaactaatt tgtgccattt ctttctctgt cagtgagaaa
    14041 tcccagatag caaaattttc ttcgatacgc tgttgatgtg tcgatttcgg aataacgacc
    14101 actccccgtt gaatattcca acgcagaatg acttgagaaa tcgatttttg atgggcatct
    14161 gcaatactct gaagcagatt attttcaaag ggtttatgtc gtccaccacc taacggagcc
    14221 catgcttcag gctgcacgtt ataatacttc atggttgcca gcgcctctgg ttgggcaaaa
    14281 tagggatgca actcgacctg gttgaccatc ggtttaactc ttaccgtttc acaaaagttc
    14341 gccagaacat gaggatagaa attggaaacc ccaattgctt ttaatttgcc ctcttcatag
    14401 gcatcttcga gtgcacgcca cacactgaaa taatcgcgca tagcctggtg caataaatag
    14461 aggtcgaagt actctagtcc cgattttttt aatgatgctt caatacctgc tgctgccgta
    14521 tcctgattca acatatcctg cccccacaat ttagacgtta taaataactc atctcgggta
    14581 cataaacctt cagatatagc ttcacgaacg gcttcaccga cggcatcttc attaccgtat
    14641 accgctgcag tgtcgatgag tcgataaccc gtgcggatag cgttcaacac tgactgtttg
    14701 cacacgtttt tatcggtaac ctgaaaaaca ccaaatccca tcatcggcat tttcagattg
    14761 ttacttaata cggaaaattc cactgtcagt ccctccatta ggcatgaaca atgagtctac
    14821 gttaaaacat aacctcaaat tagtatgtgg attttgatat cacttatgca aaaaattcat
    14881 taataatgta ggactgaaac ctctctattt tcggggacaa cgaagcagac gctaccagtg
    14941 cttttgcctt cgaccttgct gtttttgata cacttagggc cctgggcata acgaaaatgt
    15001 gcgatatcac gaagaattaa tggagaatg
//
LOCUS       DAZRVY010000098        14937 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZRVY010000098 DAZRVY010000000
VERSION     DAZRVY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.098"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHXOX_003039"
     CDS             <1..196
                     /locus_tag="ACHXOX_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006443.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            312..1610
                     /gene="kgtP"
                     /locus_tag="ACHXOX_003040"
     CDS             312..1610
                     /gene="kgtP"
                     /locus_tag="ACHXOX_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR7006444.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1607..1930)
                     /locus_tag="ACHXOX_003041"
     CDS             complement(1607..1930)
                     /locus_tag="ACHXOX_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR7006445.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1976..3331)
                     /gene="pssA"
                     /locus_tag="ACHXOX_003042"
     CDS             complement(1976..3331)
                     /gene="pssA"
                     /locus_tag="ACHXOX_003042"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR7006446.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3445..6105)
                     /gene="pat"
                     /locus_tag="ACHXOX_003043"
     CDS             complement(3445..6105)
                     /gene="pat"
                     /locus_tag="ACHXOX_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR7006447.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6137..6835)
                     /gene="tapT"
                     /locus_tag="ACHXOX_003044"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6137..6835)
                     /gene="tapT"
                     /locus_tag="ACHXOX_003044"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR7006448.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6904..7323)
                     /gene="trxC"
                     /locus_tag="ACHXOX_003045"
     CDS             complement(6904..7323)
                     /gene="trxC"
                     /locus_tag="ACHXOX_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR7006449.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7530..8567
                     /locus_tag="ACHXOX_003046"
     CDS             7530..8567
                     /locus_tag="ACHXOX_003046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR7006450.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8615..9304)
                     /gene="ung"
                     /locus_tag="ACHXOX_003047"
     CDS             complement(8615..9304)
                     /gene="ung"
                     /locus_tag="ACHXOX_003047"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR7006451.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9609..9992
                     /gene="grcA"
                     /locus_tag="ACHXOX_003048"
     CDS             9609..9992
                     /gene="grcA"
                     /locus_tag="ACHXOX_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR7006452.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="ACHXOX_003049"
     CDS             complement(10048..10635)
                     /gene="eamB"
                     /locus_tag="ACHXOX_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR7006453.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10738..11619
                     /locus_tag="ACHXOX_003050"
     CDS             10738..11619
                     /locus_tag="ACHXOX_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7006454.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="ACHXOX_003051"
     CDS             complement(11828..13162)
                     /gene="srmB"
                     /locus_tag="ACHXOX_003051"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR7006455.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13294..14031
                     /gene="trmN"
                     /locus_tag="ACHXOX_003052"
     CDS             13294..14031
                     /gene="trmN"
                     /locus_tag="ACHXOX_003052"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR7006456.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14016..>14937)
                     /locus_tag="ACHXOX_003053"
     CDS             complement(14016..>14937)
                     /locus_tag="ACHXOX_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR7006457.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3604 a         3778 c         3911 g         3644 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 atttccttta ccaaaaaaat aaacaaaagc gaccgacaaa agcatcggat tacggcagga
      301 gacataatgg catggctgaa agtactgtaa cggcagacag caaactgaca agtagtgata
      361 ctcgtcgccg catttgggca attgtggggg cctcttcagg taatctggtc gagtggttcg
      421 atttctatgt ctactcgttc tgttcactct acttcgccca catcttcttc ccttccggga
      481 acacgacgac tcaactacta caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc
      541 caataggcgg ttggctattt ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc
      601 tgctatcggt gtgtatgatg tgtttcggct cactggtaat cgcctgtttg cctggttatg
      661 aaacaatcgg tacgtgggct ccggcattat tgcttctcgc gcgtttattt cagggattat
      721 ccgttggcgg agaatatggc accagcgcca cctatatgag tgaagttgcc gttgaagggc
      781 gcaaaggttt ttacgcatca tttcagtatg tgacgttgat cggcggacaa ctgctagccc
      841 tactggttgt cgtggtttta caacacacca tggaagacgc tgcactcaga gagtggggat
      901 ggcgtattcc tttcgcgtta ggagctgtgt tagctgttgt ggcgttgtgg ttacgtcgtc
      961 agttagatga aacttcgcaa caagaaacgc gcgctttaaa agaagctgga tctctgaaag
     1021 gattatggcg caatcgccgt gcattcatca tggttctcgg ttttaccgct gcgggctccc
     1081 tttgtttcta taccttcact acttatatgc agaagtatct ggtaaatact gcgggaatgc
     1141 atgtcaacgt ggcgagtggc attatgactg ccgcattgtt tgtattcatg cttattcaac
     1201 cactcattgg cgcgctgtcg gataagattg gtcgccgtac ctcaatgtta tgtttcggtt
     1261 cgctggcagc catttttacc gttcctattc tctcagcatt gcaaaacgtt tcctcgcctt
     1321 atgccgcttt tggtctggtg atgtgtgccc tgctgatagt gagtttttat acatcaatca
     1381 gtggaatact gaaggctgag atgttcccgg cacaggttcg cgcattaggc gttggcctgt
     1441 catatgcggt cgctaatgct atatttggtg gttcggcgga gtacgtagcg ttgtcgctga
     1501 aatcaatagg aatggaaaca gccttcttct ggtatgtgac cttgatggcc gtggtggcgt
     1561 ttctggtttc tttgatgcta catcgcaaag ggaaggggat gcgtctttag tgacgggtca
     1621 gttgccagac ggtatagccg gtgcttgcac cggcgacatc ccaggccaaa tccttccagc
     1681 tccagccgct cccttcgggg cggctatccc aaagctcttt tgacgccccc aaactgacag
     1741 agaacatcaa tccaaacatg gcactgcgat cccggctcat cccctgatgc tgtgaatatt
     1801 catttccggc ggcggaaagc atcgccgagg cgataaagtg ttgagcttta tcctgcccgc
     1861 tccagctatc gttagccata tggctgcatc cagaaagtaa caatagcgaa cagacaaaaa
     1921 gaatacgcaa aacgtggcct ccaaaaaaca aaccccgtac aggacggggt tgtgattaca
     1981 ggatgcggct aattaatcgg tcgatgcgga tacggcgcaa acggcggatg agtttacgaa
     2041 ccttcaccgg ataatcggca atactttgca gatcgcgata gtgcttaacg atggtggtat
     2101 gctcgcggat cagctccagt tctttctctc gctgcggcgc aagcttcagt tgtggatcgt
     2161 ggatcaaaat ggcgttttcc agatccagac gccaggcgcg cgggttcagg ttattaccgg
     2221 tgatcaacat ccacttatca tcaacccaca tccctttcag gtgataggtg ttgtcgtcat
     2281 ctttccataa ccgaaccact agctggtcag tattgacgta atactgcaaa cggctcagga
     2341 aacgacgcag attgatctca tagagataag gcaatgcgcc aattatcttg aaaggttcat
     2401 cttccggaat gtagaagtcg ttcgcggttt tatcaccaac aataatttcg acctttttcc
     2461 cttcgcgcag caactggata atattgcgca caaggattgc tggcaggttg aagtatggcg
     2521 tacagatggt tagtttctgc tcggcacaag gcataagatg gaaaatggtc ttgttcaaca
     2581 gactcgattt ccccagcccc actagcggcg ttacggaaag ctgatcgttg tcggcatcgc
     2641 cctggaaatg ataagcggca tcacgcagct cctggcggaa cagacgaata tcgttcttga
     2701 tttccgggct ttttggccga ttaacatcat ccagacgatt aacgccgcgg ccattcataa
     2761 tattctgtgt aacccattca aacataatgt ctgacatctt acggttacgg ataagatgat
     2821 aacggtcgta gcgatattta tcgtgctgat gcaggtaaac atcgttcagg ctggcaccgc
     2881 tataaagtac gctatcgtcg atgataaagc ctttaaagtg cagaacacca agggcttcac
     2941 gggtattgat tggaacgcca taaaccggaa catctacgcc cgaattttcc tgcgccatgc
     3001 ggcagtacca gtcagcgtta gtgttagatg ccgcagcgcc aatgcgtcca cgttgtgcac
     3061 gatgccagtc gaccagcacc cgcacatcca gttccggacg ctgcctttta gcttcataca
     3121 acgcgttcag aatgcctttg ccaccgtcat cctgttcgag atacagggcg acaatgcaaa
     3181 tgcgctgctt cgcgctggct attttttcca gcagcgtctc ccggaagtcg gcgggagcgt
     3241 aaaagaaatc gacatcatca actgattgag aaatcttggg tagttgggca aggtgttgtt
     3301 gatgtttatt acgcttaaat tttgacaaca tcacagtgca tttcttctct gttcattgaa
     3361 gggtcctctg tgcaatgcag acgacataag cgggcaataa taacaccagt cccgattaag
     3421 tggtcaacat ttccagtacc ttactcatga ttcctcgcgt tgggcaagat ttagcgtaag
     3481 cccaacgatc ccctcttcga gctggatatc aacgttaaac ccgagcttgc gggctagcgc
     3541 caccatgcca cgattgtttg gcatcgtaat accattcaga cgttgtagtc cgtgatctcg
     3601 cgtataggta atcaactttt ccattaagcg tcgacctaag cctaaccctt tgagatccga
     3661 gcgaaccagc acagcaaatt cggcatcgat gttatcagga tcggaaatcg cacgcgtgac
     3721 gccgaggatc tcttccgttt gatcaatacg tcgtaccgct acaaacgcca tttcccgatc
     3781 gtagtcgatc tgtgtcatgt tggctaaatc ttcatgggta aattcgttga tctcgctaaa
     3841 gtagcggtaa taaagatctt ctttggtgac tcgcgaaatg aattgctgaa gttgtggctc
     3901 atcttctggc aaaatcgggc ggaacaagca gcgttcaccg tttttcaatt ctacccattc
     3961 ttccagctga tgcggataag ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg
     4021 cgagatatcc agcgtgacat ccagcgcggt aaattcactg ccagaagcca gcaaaggatg
     4081 aatatccaga cgctgaattt ccgggcaatc gacaatcaag ttggaaacct gcaccagaag
     4141 ctggctcaag cctgcaacat ccaatgggcg tagcgcactg cgcgcacgaa tctttttact
     4201 tttgatcccc tgaataacca gatagcgggc caggttcatg ttcagcggcg gcagtgcgac
     4261 gacggcttga tcttcaggac gccactccac accgccttca cccagcatga tcaacggccc
     4321 gaaaaccgga tcgtgctcaa ccacaacccg caactcctga gcgccagcac ggttagccat
     4381 actttgcacc aacaggccgt ggacccgcgc ctgtggccag gccattttta cgcgatcgaa
     4441 aatagcgttc gccgcttgct ggacttcatt ggctgtacgc aggtaaagca tgacgccctg
     4501 aacttccgat ttatgtggaa tatccggcga acgcaatttc agcgccaccg gataaccaat
     4561 ctgttcggca atatgcaccg cttcggtgct atcgctggca atccaggtag ggagcgtgtt
     4621 catgccatac gcttgcagga tgggctgaac ttcatgggta tcgagcgacg tagccccttc
     4681 ggcaatcgcc tgttgcaaca gaagatgcgc ttctgcggta ttggaagtca gattgctggg
     4741 caacgtcggc gtttcgcgta gttgcttctg attacgccgg tactccacca tatgcataaa
     4801 agcagtgatg gttccttccg gggtacggta ggtcggcagc ccggcttcgc tgaataaacg
     4861 tcgtgcctct tgcgaggagt gctcgccgca ccagttcgtc agcaaagaga catatttgct
     4921 gcggggatga tgctttaccg cttcaattaa tacttgcgcg ctttctgttg cgggagcagc
     4981 ggcgctgggc gaatgaataa ccatcagcgc atcaaaatcc tggctgtgga gcagaatgtc
     5041 cagcgttttg acataatgct cgctgctggc gtcatcgcgc agatcgagag ggttagagac
     5101 tgccacatgt tctggcaatg catcgcgcaa tttctggcag gtttcttcgc ttagcgttgc
     5161 cagcttgcca ttgcgtgacc ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt
     5221 gctgataatc atcaaccggt cgccacgcag cgggcgcata tggctaaggg tttccaccgc
     5281 cgaaaacagt tcgtgggtat cctgtacccg caacaaaccg gcacgctgaa tagccgcatc
     5341 ccatgccggg tccattcctg ccgtcgtgtt gagcagtcgc tgtgccgccg ggctacgtcc
     5401 gcttttaatc accagaatcg gtttattacg cgaggcacta cgggctgccg aaacaaagcg
     5461 tcgcgcgtcg cttaactgtt cgagatagag caggatggcg ctggttttac tgtcgcgcgc
     5521 cagatagtcg agcaattcat caacgtcgat atccaggctg tcgccgagcg caataaagta
     5581 ggaaaagccc atctcacgct gttgcgccca gtcgaggatg gtgttggaga cggctgccga
     5641 ttgtgaaata aacgccagct tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag
     5701 accttgccag ggagccagta aacccagact gtttggtcca agcaggcgca tgttatggcg
     5761 cagggcgcag gcgcggagat cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt
     5821 tttacagcct ttctcgccga gctcttccag aagagcaaga ttacggctgg cattggtaca
     5881 taaaaccgca aggtcgggtg taaagggcaa gctggcaata tccggccagg ccaacacacc
     5941 caacaccgct ttccaggctg gcgtcaccgg gagtaccggt ccgttaaagc ttcccgccag
     6001 caggttacgc atcatcaggt aacctgcgcg attgggtttc atcgacgcgc caattaccgc
     6061 tatcgatttt ggtcgcagta gtgcttccag tcctcgctga ctcataccgg tctcccgcag
     6121 aagtgaccga atgattttaa acgctttcta actgttctgc tgtgatgcta cccagatgtt
     6181 gcgtttttcc tgccagatag cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg
     6241 ccgccccggt atcgccggcc atatctaaca gtgcgatggc tacctcggca gtacaatatt
     6301 ggcctgcagc ctgggcttca cgcaggcgat aggcagaaag ccgggaaaga tcgacggaaa
     6361 tgacgggaag attatccaga tacggacttt tacgaaacat cttgcgagct tccggccagg
     6421 taccatcgag catgataaac agcggtggct taccggcagg tggtgtgaag atcacttccc
     6481 gttgctcatc agcatacgag gcgggaaaga ccaccattgg ctgataatcc gggttttgta
     6541 ccagatccag caaatcctgc gagggttcgg tacgcgacca ttgaaacgca acggtatcag
     6601 gcaaaatatc agcaatcaga cgcccggtat tactgggctt cattggctcg gtgtcgaaca
     6661 tcagcaaaca gaagcgactt tttgcttgtg ctggggtaat tgtcgaacag agacataatt
     6721 tctctggcaa aagacagcgt tggcagcgat gaacgcgatt accgcgggca agaaaaggac
     6781 gtgttgcgcg cgcaatacgc tcggcgcgta actggagaac agcgttttcg gtcataagag
     6841 agcgtcgaaa aaacgccatt gtcgcagagg agaaaacggg gcacaagatg caccccggta
     6901 agattaaaga gattcgttca gccagctatc gaacggcgct ttcggtactg cgccattaag
     6961 catgtcgaca acctgaccgt ttttgaaaat catgatcgtc ggtatactac gaattccaaa
     7021 gcgactgctc aattcacgtt cagcttcggt attcactttc acaaagcgca ctttaccgct
     7081 acgctcttgc gcgacatctt caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg
     7141 tgcccagaag tcgatcacca caggtagatc atccttcagc aatttgtcga gtgtttcacc
     7201 ggtcgcatta atcacctctc cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc
     7261 atcttcgatc cgatcgtcgg gaatgcgatt gatggcctga caatgggtac aaacggtatt
     7321 cataactaac ctcgggatga gtaaggagac ttatcggcaa cgacgccggt atgtttctaa
     7381 tatgttacat attatcgggg acattgttcc agacaacaat ctgttttatt caataggtat
     7441 gatagtcgca ggctttggta cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg
     7501 cgcttcgcgc agcgctggtg gagaaaagca tgaacgatga aatgaaaggt aaaagcggca
     7561 aggtcaaagt gatgtatgtc cgcagtgatg atgattctga taaacgtacc cacaacccgc
     7621 gtaccgggaa agggggcggg cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg
     7681 cccgcgatga caaacagagt cagccccgtg accgcaagtg ggaagattcg ccgtggcgca
     7741 cggtttcccg cgcgccgggt gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg
     7801 gtaaaagttt tattgatccg gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct
     7861 acggcgaaaa tgcctgtcag gcactgttcc agagccgtcc ggaagcaatt gttcgcgcct
     7921 ggtttatcca gagcgtaacg ccacgtttta aagaagcctt gcgctggatg gcggcaaacc
     7981 gcaaagcgta ccatgtggtg gatgaagctg aattgacaaa agcgtcaggc acggaacatc
     8041 acggcggcgt ttgcttcctg atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa
     8101 gccaggcagg cgcgcaggat tgcgttctgg cactggaaaa tgaatctaac ccgcataacc
     8161 tgggtggcat gatgcgcagc tgcgcgcact tcggcgtgaa aggtgtcgtg gtgcaggatg
     8221 cggcactgct ggagtcgggg gcggctatcc gtaccgcaga aggcggcgca gagcacgttc
     8281 agccgattac tggcgacaac attgttaacg tgctggatga tttccgtcag gcaggttaca
     8341 ccgtagtgac cacttccagc gagcagggta aaccgctgtt caaaactagt ctgccagcga
     8401 aaatggtact ggtgctgggt caggaatatg aagggttacc ggatgccgca cgcgatccga
     8461 acgatctgcg cgtgaagatt gacggtacgg gcaacgttgc cgggctgaat atctctgtcg
     8521 caaccggcgt tttgcttggt gaatggtggc gtcagaataa agcctgataa atcagccggg
     8581 tggcaactct gccatccggc atttccccgc aaatttactc actctctgcc ggtaatactg
     8641 gcatccagtc aatcggcgtc tcgccacgtt gttccagcca ctgatttgcc agcacaaaat
     8701 ggttgcagcc aaagaatcca cgatgcgccg aaagcggcga cggatgcggt gctttcagta
     8761 catgatggcg ttgcttatct ataatcgccc ctttcttttg cgcatgtgat ccccacaaca
     8821 aaaacaccac gccttcgcgg tgctggttaa tcaggctgat gactttatca gtaaacgtct
     8881 cccaaccgag gctggcgtgg gaatgcgcct gacctgcgcg aaccgtcaac acagtattga
     8941 gcagcagaac gccctgacgc gcccagcttt cgagatagcc atgattaggg cgggtgaagc
     9001 ccggaatagt attttccagc tctttataca tattcaataa tgacggagga atggcaatgc
     9061 cgggacgaac ggaaaatgcc agaccatgcg cctgtcccgg tccgtgataa ggatcctggc
     9121 cgagaatcac cactttaacg tcacccaact cggtaaagcg gaacgcgtta aagacatctt
     9181 tttgtggtgg atagatagtg acgccggact gccgctcgct ggcgacggtc tgaagggtat
     9241 taagaaaata aggttgttgc ttctcttcag ccagcacgtc atgccaggtt aattcgttag
     9301 ccatctcgct ctcctgcgaa tcttcaatcc gcctagctta actgcttctt tctacagaac
     9361 aaaatcatgc tcatttttta gacttaaacg agtgcaaaaa aatgtaaatt ggtaaaattt
     9421 cacacccgca taagttgatg taaaacaata aaatccattt acgaccacaa tcgatgtgtg
     9481 gtttttattg atttaaatca aagaatcaag ggtggttgag gagtatatat acactcaagc
     9541 aacaatggtt ttaccaattg gccgcgacag gctgaacaaa tcaaataatt ttgccgggga
     9601 ggcatcacat gattacaggt atccagatta ctaaagccgc taacgacgat ctgctgaact
     9661 ctttctggct gctggacagc gaaaaaggcg aagcacgttg catcgttgca aaagcaggtt
     9721 atgcagaaga tgaagtggtt gcagtaagca aactgggtga cattgaatac cgtgaagttc
     9781 cagtagaagt gaaaccagaa gttcgcgttg aaggtggtca acacctgaac gttaacgttc
     9841 tgcgtcgtga aactctggaa gatgcagtta agcatccgga aaaatatccg cagctgacca
     9901 tccgtgtatc cggttatgca gttcgcttta actctctgac tccggaacag cagcgcgacg
     9961 ttatcgctcg tacctttact gaaagcctgt aaggcttaag tagcaagtta ctaagcggaa
    10021 gagggcgacc tcttccgctt tttttcgtta atagaaaatg cgtaccgcgc aatagaccag
    10081 taacagggca agcacgatat ttaactggcg accatactgg cgaaacaatc gctgaaacag
    10141 atgccccgcc agcgcccagc acacattgcc aaacgtccca atcatcgcca gcaaaacgct
    10201 gacgccaact acccagctta gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt
    10261 aacaccgtac aaaatgattt tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct
    10321 gattggtttt gcctgaagtc cgtcttcctt tgttgggctg gtggcgattt tccacgccag
    10381 ccagacaata tatgccgccc ccgcccaact caaaaggtgt accgctgccg ggtcaatcac
    10441 tgccagtgaa aatgaaatgc ccgcacacag taacatcaca atcaaaaatc ccagactcat
    10501 ccctgccagc acgcgggtac tttgacgaaa tccatgcgac gtagcagagc taagggcgag
    10561 aatattgttc ggtcctggcg tcatagcggt aatcagggtg taagtccaaa aagcacttaa
    10621 aagggtcggt gtcacttgtt atctcctctg ttttggtaga cagtacccga cacagaacat
    10681 ttaaaaaaat gaatgttttt atattctaga ttcgatattt tcgataggtt tggggttatg
    10741 gatctgcgcc gttttattac gcttaaaacc gtggtggaag agggttcctt tttgcgagct
    10801 tcgcaaaaat tgtgctgtac acaatcgacg gtgacttttc atattcagca gcttgagcag
    10861 gagttttcag tccagttatt tgagaaaatt ggccgacgga tgtgccttac ccgcgaagga
    10921 aaaaagttgc tgccgcacat ttatgaactt acccgagtga tggatacgct tcgtgaggct
    10981 gccaaaaagg agtcagatcc ggatggggaa ctacgcgttg tttcgggcga aacgttgctc
    11041 tcttaccgaa tgccgcaggt gttgcagcgt tttcggcaac gcgcaccaaa agtgcgttta
    11101 tcgttgcagt cgctgaactg ttacgtgatc cgtgatgcgc tactgaatga tgaggccgat
    11161 gtcggcgtct tttatcgcgt agggaatgat gatgccctga accgacgaga gttgggtgaa
    11221 caatcgctgg tgctggtggc ttcaccacaa attgtagatg tcgattttac tgagccggga
    11281 agacataacg cctgtagctt tattatcaat gaaccacaat gtgtcttccg gcagatattt
    11341 gagagcacgc tgcgtcagcg gcggatcacg gtggaaaaca ccattgagct tataagtatc
    11401 gaaagcatca agcgttgtgt cgcggcgaat atcggagtca gctacctgcc gcgttttgcg
    11461 gtggcaaaag agttggagtg cggagaatta attgaattgc cttttggcga acagtcgcag
    11521 accattacgg ccatgtgcgc acaccatgct ggaaaagcgg ttagcccggc gatgcacact
    11581 tttattcagt gtgttgaaga gagttttgtg gctggataaa aataatgtcg ggattaaacc
    11641 cggcatttaa cttgtcgaca aagtgcgctt tattcatgcc ggatgcggcg tgaacgcctt
    11701 atccggccta caagatcgtg caaattcaat atattgcatg agccacgtag gcctgataag
    11761 cgtagcgcat caggcagttt tgcgtttgcc agcactttta ataccaggct taaacccggc
    11821 attgagatta ctcttctgtc gtttgcggtg gcacgcccgt tccgcttggt ttacggcgct
    11881 taccaatatt tttggtgtcg cgatggcgtt ttttcacccg cggtttctct ttctctttag
    11941 cttttttctt ctcggcgcgt tttgccagta ctttcttcga tggcttgccg gtctgcttct
    12001 cgcttggcgc acgcgttttc gggcgtaact catcaataac gcgagcttta atcggctctt
    12061 caatgtaacg gccaaccttg cccagcagca aatggtcatg ggcttccacc agcgaaattg
    12121 cggtgccttt acgaccggcg cgcgcggtac gtccgatacg gtgcaaataa gtatcgccac
    12181 tgcgcggcat atcgaagtta aagacgtggc tgacgtcagg aatgtcgata ccgcgcgcgg
    12241 caacatcggt tgcaaccagt acgtttacgc gaccttcggt caaacgcttg atcgcttcgt
    12301 tacgcttgcc ttgtaccatc tcaccttcga gatagcagtt gttgatgccc gcttcgcgca
    12361 gccagtttgc cagctcatgc acacgttcac gcttacgcac aaacacaatt gagcgggtcg
    12421 cttccggctg ttttaacaga tgcaccagca atgcggtttt atgttccagg tcatcggcgc
    12481 ggtaatacca ctgatgaatt tttttgcgct cacgggtgga cggattggca gaaacctcca
    12541 ccggatcttc cagcagacgc tcggcaaagt cctgaatcgc atcgccttcc agcgttgccg
    12601 aaaagagcag ggtctgttta cgccagcgcg tttcgccagc aatatgttcg atatcctgag
    12661 cgaagcccat atccagcata cggtctgctt cgtcgaggat cagcgtttca accgcgcggc
    12721 aatcgaagtt ctcttctttt atgtattgca gcagacgtcc ggtcgtggcg accacgatgt
    12781 cctgattttc gctgaacact tccgcgtggt tcatataggc tacgccgccg gtgatggtgg
    12841 cgatatccag atgcgtatgt ttcgccagtt cgcgggcatg atcggccacc tgcatcgcca
    12901 gctcgcgagt tggggtgagg atcaaaatac gcggcggacc ggatttctta cgtgggaaat
    12961 cgagcaggtg ctgcaacgct ggcagcagat acgccgccgt tttaccggtg cctgtcggcg
    13021 cagaaccgag tacatcacgg ccatcgagcg caggcggaat ggcggcagcc tgaatggcgg
    13081 tcgggcgagt gaaaccttta tcctggaggg cttccagcag gctttcgtcg agttcaagtt
    13141 cggaaaaagt cgttacagtc atgttctacc tctgtgtggg gcgctgatta tagacgttac
    13201 ggctgcaatc ttcatctgtt tgtatggata tcgcttctcg ggtattgttc tcacccggtg
    13261 ctatcgccgt ttttcctgca aggttttatt ttcatgtcac agtctacatc cgtgcttcgt
    13321 cgtaatggat ttacttttaa acagtttttt gttgctcacg atcgctgtgc gatgaaagtg
    13381 ggaacggatg gcattttgct gggcgcatgg gcaccggtgg ctggggtgaa acgttgcctt
    13441 gatatcggcg cgggtagcgg gttgctggca ctgatgctgg cgcagcgaac cagtgacagc
    13501 gtgataattg atgccgttga gctggaaagt gaagctgccg cgcaggcgca ggaaaatatc
    13561 aaccagtccc cgtgggcaga gcggattaac gtccatacgg cggatattca gcagtggctc
    13621 acacagcaga cagtacgctt cgatttaatc atcagtaacc caccttacta tcagcaggga
    13681 gtagagtgcg cgacacctca acgggaacag gctcgctata ccactacgct tgatcaccca
    13741 tcgctgctga cctgtgcggc ggagtgcatt accgaagagg gatttttctg cgtggtgctg
    13801 ccagagcagg ttggtaatgg ttttactgaa ctggcattaa gcatgggctg gcatttacgt
    13861 ttgcggacgg atgtggcgga aaacgaagcg cgactgccgc atcgggtgct gctggcattc
    13921 tccccgcagg cgggagaatg ctttagcgat cgcttagtca ttcgtgggcc agaccagaac
    13981 tattccgaag cgtatacggc gctgacccag gctttttatc tgtttatgta atgattgccg
    14041 gggggaagga tcgatggacc ggaatgcgtg agcagttccg ggtaatccag cgtaaaatgc
    14101 aggccacgac tctccttgcg catcatcgca cagcgaacaa tcaactcggc aacctgtacc
    14161 agattacgca gctccagcaa attattcgac acgcggaagt gggcgtaata ttcgtctatt
    14221 tcctgttgca acatattgat acgccgcagg gcgcgctcaa gacgttttgt tgtgcgcaca
    14281 atgccgacgt aatcccacat aaacagccgt agctcgtgcc agttatgctg aattactacc
    14341 cgttcgtcag ggttctcaac gcggctttca tcccacggtg gtaacgtact gacgccgtgg
    14401 gcatcaggca tacgtctggt gatatcttct gccgccgacc agccatagac cagacactcc
    14461 agcaatgaat tcgaggccat gcggttagcg ccgtgtaagc cggtataact cacctcgcca
    14521 atggcataca agccctcgac gtccgtacgc ccatgatcat caaccattac accaccgcag
    14581 gtatagtgtg cagcaggcac aatcggtacc ggttcttgtg tgagatcaat ccccagcccg
    14641 agcaattttt cataaatcat cgggaaatgc tggcgaataa aatcggcggg cttatggctg
    14701 atgtcgagga acatacaatc tgcgccgagg cgtttcattt catggtcaat ggcgcgggcg
    14761 acaatatcgc gcggggccag ttcgccgcgc tcatcaaaat cgggcataaa acgcgtgcca
    14821 tccgggcgct tgagataagc gccttcgccg cgcagtgctt ccgttaacag gaaattgcgt
    14881 gcctgtgggt gatacagtgc ggtagggggg aactgattaa attcgagatt ggcaacc
//
LOCUS       DAZRVY010000099        14575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZRVY010000099 DAZRVY010000000
VERSION     DAZRVY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.099"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            462..809
                     /locus_tag="ACHXOX_003054"
                     /pseudo
     CDS             462..809
                     /locus_tag="ACHXOX_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            862..1257
                     /locus_tag="ACHXOX_003055"
     CDS             862..1257
                     /locus_tag="ACHXOX_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR7006458.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1298..2041
                     /gene="cmoA"
                     /locus_tag="ACHXOX_003056"
     CDS             1298..2041
                     /gene="cmoA"
                     /locus_tag="ACHXOX_003056"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR7006459.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2038..3009
                     /gene="cmoB"
                     /locus_tag="ACHXOX_003057"
     CDS             2038..3009
                     /gene="cmoB"
                     /locus_tag="ACHXOX_003057"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR7006460.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3174..5603)
                     /gene="torZ"
                     /locus_tag="ACHXOX_003058"
     CDS             complement(3174..5603)
                     /gene="torZ"
                     /locus_tag="ACHXOX_003058"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR7006461.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5628..6728)
                     /locus_tag="ACHXOX_003059"
     CDS             complement(5628..6728)
                     /locus_tag="ACHXOX_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR7006462.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(7116..7862)
                     /gene="cutC"
                     /locus_tag="ACHXOX_003060"
     CDS             complement(7116..7862)
                     /gene="cutC"
                     /locus_tag="ACHXOX_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR7006463.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7876..8442)
                     /locus_tag="ACHXOX_003061"
     CDS             complement(7876..8442)
                     /locus_tag="ACHXOX_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7006464.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8658..10394
                     /gene="argS"
                     /locus_tag="ACHXOX_003062"
     CDS             8658..10394
                     /gene="argS"
                     /locus_tag="ACHXOX_003062"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR7006465.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(10436..10480)
                     /gene="yecV"
                     /locus_tag="ACHXOX_003063"
     CDS             complement(10436..10480)
                     /gene="yecV"
                     /locus_tag="ACHXOX_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR7006466.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            10571..11059
                     /locus_tag="ACHXOX_003064"
     CDS             10571..11059
                     /locus_tag="ACHXOX_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR7006467.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(11179..11571)
                     /gene="flhe"
                     /locus_tag="ACHXOX_003065"
     CDS             complement(11179..11571)
                     /gene="flhe"
                     /locus_tag="ACHXOX_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR7006468.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(11571..13649)
                     /gene="flhA"
                     /locus_tag="ACHXOX_003066"
     CDS             complement(11571..13649)
                     /gene="flhA"
                     /locus_tag="ACHXOX_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR7006469.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(13642..>14575)
                     /gene="flhB"
                     /locus_tag="ACHXOX_003067"
     CDS             complement(13642..>14575)
                     /gene="flhB"
                     /locus_tag="ACHXOX_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR7006470.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         3576 a         3833 c         3489 g         3677 t
ORIGIN      
        1 ttccttatga gactggttaa tagagcgcta tgaagattca ggagggtaac tataaccatc
       61 actttctaac tttcacgtag gaaatcgtta agtttcttga tagttacacc atgtgaccaa
      121 ctatctatat atcgttatat agtcctgtat atagcactat atgtagtgtc tggagaggga
      181 cgactaacac gatacagttt accctgaaaa gagacttcac agattcttta tatatttcaa
      241 taagttatgc taaaaagaga ctataccgat actgcataga atttgtgtag gattgcatag
      301 atacttggaa aaacaccgtt aaattgcata gtcattcacc tgatattgtc ctgtttttac
      361 aggtagttga ttcggatgtt ctaggaatca tcctcatagt tgattcggtt agttgggctt
      421 gtaccaaccc ctaatcaacc tcatagtgta tttagacctt gatgatttta gacagccgcc
      481 ctgttcatgc agcacgtcca cacagtgaag ctattcgcga cgctcaacga aaaaaaccta
      541 aagttccggt acatgctgta ctgacggcga caaatccgct gattcgtttt atcggtagtg
      601 atgatatgac gcaaaaccgg gaattatttc aggtctggtt acagaaatta gcgcagtggc
      661 atcagaccac tacgccttat ctttttttac atacgccaga cattgcccag gccccggaac
      721 tggtacatac cctgtgggaa gacttacgta aaacgcttcc agagatcgga gcagttccgg
      781 ctattccaca gcaatcttct cttttctgaa tttgccacct atcatagaca ggtgccatcg
      841 gccattttaa agggagtttg tatggtaagc gcgctatatg ccgttttaag tgcgttgtta
      901 ttaatgaagt tctcttttga tgtcgttcgc ctgcgaatgc agtaccgcgt tgcctatggt
      961 gacggtggtt ttagcgaact gcaaagcgct attcgcattc atggtaacgc ggtggaatat
     1021 attcccatcg cgattgtgct gatgctgttt atggaaatga atggcgcaga aacctggatg
     1081 gtgcatattt gcggcatcgt tttgcttgct ggtcgtctga tgcattatta cggttttcat
     1141 caccgtctgt ttcgctggcg acgttccggc atgagcgcca cctggtgtgc gctgttgctg
     1201 atggtgctgg cgaatctttg gtatatgccc tgggagttgg ttttctccct gcgttagcgc
     1261 acaatacgcc actttctttt tcccggattt ttacgttatg tctcaccgcg acacgctatt
     1321 ttctgcccct atcgccagac tgggcgactg gacctttgat gaacgggtag ctgaagtctt
     1381 cccggatatg atccagcgtt ccgttcccgg ctattccaat attatttcca tgattggtat
     1441 gttagccgag cgcttcgttc aacctggtac gcaggtttac gatctgggtt gttctctggg
     1501 cgcggcgacg ctctcggtgc gtcgcaacat tcatcatgat aattgcaaaa ttattgccat
     1561 cgacaactcc ccggcgatga ttgaacgctg cagtcgtcat attgacgcct ataaagcccc
     1621 tacgccagta gacgtcattg aaggtgatat tcgcgatatc gccattgaaa acgcatcgat
     1681 ggtggtgctg aattttaccc tgcaattcct ggaaccttcc gagcgccagg cattactgga
     1741 taaaatttat caaggactga acccgggcgg cgcgctggtg ctttcggaaa aattcagttt
     1801 cgaagatgcc aaagttggtg aactgttgtt caacatgcac cacgacttta aacgtgccaa
     1861 cggttacagc gaactggaga tcagccagaa gcgcagcatg ctggaaaacg tgatgctgac
     1921 cgattccgtg gaaacccata aagcacgcct gcataaagcc ggttttgagc atagcgagct
     1981 gtggttccag tgctttaact ttggttcact ggtggcatta aaagcagagg acgctgcatg
     2041 atcgactttg gtaactttta ttctctgatt gccaaaaatc atctttcaca ctggctcgaa
     2101 acgctgcccg cgcagattgc taactggcag cgcgagcagc agcacgggct gtttaagcag
     2161 tggtccaacg cggtggaatt tctgcctgaa attaaaccgt atcgtctgga tttattgcat
     2221 agcgtaaccg ccgaaagcga agagccactg agcaccgggc aaattaaacg cattgaaacg
     2281 cttatgcgga acctgatgcc atggcgcaaa gggccgttct cactgtatgg cgtcaacatc
     2341 gataccgaat ggcgttccga ctggaaatgg gatcgtgttc tgccccatct ttctgattta
     2401 accggacgca ccattctgga tgtcggctgc ggcagcggtt atcacatgtg gcgcatgatt
     2461 ggcgcaggcg cgcacctcgc ggtggggatc gaccctacgc aactgtttct gtgccagttt
     2521 gaagcggtgc gtaaactact gggtaacgat cagcgcgcac atttgttacc gttaggtatt
     2581 gaacagcttc cggcactgaa agcctttgat accgtctttt cgatgggcgt gctttaccat
     2641 cgccgttcgc cgctggagca tctctggcag ttgaaagatc aactggtgaa tgagggtgaa
     2701 ctggtgctgg aaacgctggt tattgatggc gatgagaaca cggtgctggt accaggcgat
     2761 cgttacgcgc aaatgcgtaa tgtctatttc atcccttccg cgctggcgct gaaaaactgg
     2821 ctgaagaagt gtggttttgt agatatccgc attgtggatg tgtgcgttac cactactgaa
     2881 gagcagcgac gcactgaatg gatggttacc gaatctctgt ccgatttcct cgacccacat
     2941 gatccgagta agacagttga aggttatcct gcgcctaaac gcgcggtgct gattgcgcgc
     3001 aagccgtaaa ggtctggtat tactgccgga tgcggcgtga atgccttatc cggcctacaa
     3061 acccttgcta attcaatcta ttgcagggac tatgtaggcc tgataagcat agcgcatcag
     3121 gcagttttac gtttgcataa cctcagcgcc cgtttccggg cgctattcac ttcttacgcc
     3181 tgaacagcag gcttatcaaa tgccgttaac ttcggtgcgt tacccgtata tttttcgata
     3241 tacaccagcg cagagttacc ggcacaggca tttgccagct gcgagctggg gatatccgcc
     3301 gttaacacgt tcgcactgcc gtttttacac aagccgtttt ccagatctgg ccatgcacct
     3361 tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca ccaccgcgcc tgtcaaaatc
     3421 tgaccacgtt tgttccagac acgcaccaga tcgccattcg caataccaaa tcgagcagca
     3481 tcttcggtgt gaatagtgat cggttcacga tcggcaaccg catatttttt acgcagttcc
     3541 gcatagttaa gctgactgtg taaacggtgt gccggatgtg cggtcagaag ctgcaactgc
     3601 ttctcgtcgg cggtaccctt ccactcatca ggcgcaagcc aggttgggtg tgccgggcaa
     3661 tccttatagc caaatttttc cagcgttttg gagtaaatct caattttgcc acttggcgta
     3721 cccagcgcat ttttcaccgg atcggcgcgg aaatcaccat aacgaacgta ctgttcgttc
     3781 ttctcgctgc ggcgcatttc gatcagttta ttttgctgcc agaaggcatt aaacattggc
     3841 atagtgacgc gttgcgcacg ggcccctttc tgagcggcat catagaaaaa tttcagccac
     3901 gccatttcat ctttaccttc ggtatagatc tcttttccgc caggtttgag taattccgca
     3961 agatcggcaa acacatcaaa atcgtttcgc gcttcaaatt gcggagcaac agcctgcttc
     4021 atcggcacaa tatgctggtt gctgtaatca ccggtcatcg tcaagtcatt gcgctcaaac
     4081 gatgtggtga tcggtaatac gatatctgcg tgtttagcag ctgcggtcca gtagcattca
     4141 gaaacgacga tcatctccgg tttctgccat gctttaatca gacgattagt atcctggtga
     4201 tgggtaaagt taccgccacc cgcccaccag atcattttga tattcggata cgtttgctct
     4261 ttaccgttat gctgatattt gccgccagga ttttccagcg catcgacgat acgggcaaca
     4321 ggaatcgccc tcattccgcc atcatcagcg gcctcgctgg cctgtccggc aatcgccgca
     4381 gacatttccg gcagcacgcc gccaacacgt gtcgggttgc caccattgga gtaatgataa
     4441 gagaaaccaa aaccgccgcc cggtgtacca atctgcccta acatcgccgc cagcgtcacc
     4501 agcatccagt gtttctgttc accatattgc tggcgctgaa ttccccagcc tgccatcagc
     4561 attgtgcgat tcgccgccat cagttcagcg agtttgacta tctgcgcttc cggtacgcca
     4621 gtaatttccg ccgcccaggc cgcgctcttc ggcgtattat cgctcttacc tctcagatac
     4681 tcttcaaact gcggataccc ggtagtgtat ttttcaagga acactttatc gtgtttgcct
     4741 tgtgtcatca gggtatgcgc aatccctaac atcagtgcca cgtcggtgcc catattcggc
     4801 gcgatccagg tggcattatc gccaaagaag tcgatggttt cggagcggat aggatcaatg
     4861 gcaatcactg gtttgccaga ttttttcagc tgatggaagt attccagccc ttgctcatcg
     4921 gtactgctcc aggcaatttt taaggtattc agcgggttca tcccccacag caccacaacc
     4981 tggctgtttt ccagaatcag cggccaggag gtctgctgtt catacacctc tacagaaccg
     5041 accacatgcg gcatgatcac ctgtgccgcc ccggttgaat aatcgccgct atgcccggaa
     5101 taaccgcccg ccaggttcat ataacgttgc agtaaggttt gcgctttatg caacacgcca
     5161 gaagagcgcc agccgtatga tccggcaaaa atagccgatg gtccgttagc tttgcgaatg
     5221 cgatcgtgtt gctcatgaat cagttttaat gcttgttccc agctcacctg tacataggta
     5281 tcttcgccac gacctttcgt cggttgcagt ggattatcga gatagctttt tctcaccatc
     5341 ggatgctgaa tacgcgtcgt ggtgtgtacc tgatccgccg ccgtagactg taaggaattc
     5401 ggtatggttt tcgccagcgc gcctgttgaa gaaacaatct tgccgtcctt cacttctacg
     5461 ttcatcgctc cccaacgtcc cgcggtgagg attttaccgt ccttctcttc tgcccatgcg
     5521 gggagcggtg ctgccgatgt caccaccagc gctccagcgg caataccgct gtgtttaata
     5581 aattcacgtc ttgttaatgt cataacttcc tccctgatca acgaggatca ctgtttctcg
     5641 gtaatatctt tggcgttgta ctggaaatac cgcgttaaaa tgtccagttc gttttcgctc
     5701 atgctggttc gtgcccccat tcctttggca atggacggcc acgcattgac ggtgtaatgg
     5761 tcggcggcaa taggggcatg acaaccagcg caataggtat cgtcaagttt ttcagcgtat
     5821 tgccatagcg gtttacggtc cgctaatccg ggatcggtaa gcgcaccctg taaagacgcc
     5881 tgacgccatt gattgccgta ttcgtcagcc tgccattccc cgtttaccgt gagcgccttg
     5941 ataccttctt cacttaatgt ggctagcgcc agccgttgac ctgccgccag gtagagcgtg
     6001 ttttcactgc cctgcatttg ataaccctgc aacagaacga tcggctgttt gccactggca
     6061 tcaacgacgg tgagatcggt accaggattc acggtagcca gctcgcctat gcgagaagtt
     6121 ttgaaaggat aaatatgtgc gccattagtc actgaagtag cggcctgact ttccagctca
     6181 tgcgccgcgt tgtcatccat ttttatttct ggcggaaaat gggcaatgcc tttatgacaa
     6241 tcgatacagg tttcgccgcc cttttgtgct ttgttatgca ttttctgcgc agattcactt
     6301 tgcgaggcaa tatccatggc atcaaaagaa tggcaactac ggcacgttgc agagtcagtt
     6361 gcttttaatt ctttccatac tgtttcggcc atttcctggc gatgagtttc gaacttatcg
     6421 tcactgtcta ttttgccgct aacaaattca tgataaatat ctttagatgc ttttaattta
     6481 gcaaataaat aatccatccc tgactttgga atatggcaat ctgcacattc cgcacgtatc
     6541 cctttctggt tcgaaaagtg gacagttccc tgatattcct caaaaggttt actcatcgag
     6601 tggcaggaaa ggcaaaatgc tgtatccgac gttttatgta aggctttttg cgccagcaat
     6661 aacccgccgc caccaaccac aaccgctatc agcaaaaaca ataacccaat gcgttttttc
     6721 cctcgcatag attcctctaa ataaattcca gaacaccgaa atcattgtat aattaaatac
     6781 acaacgaatt aaccgcttgc ataactcggc acaacactgc ctgaaataat cgataaagaa
     6841 tatgatttat tacaatgtaa tcattaattg ctaaggaata acccagttgc tatttagaat
     6901 atatatttat cagttttagc aatttaaatc ccataatcaa tgtgaatata aaaaatatta
     6961 ttagcacaac gctatgttca ctgtcacccg ctcaacagat cacggttatc gttcgttttt
     7021 tatactgctc agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa
     7081 gcatcatatt gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg
     7141 attcctttca tttcagcaac cgccgccccg tctacgatat aacgcgaata ctcgtccgcg
     7201 tgttcatctg atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac
     7261 gctcccgcgg agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct
     7321 gcacggactc ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata
     7381 atttttgata aaccttgcag tgcgtctgat ttttgccctg atgtcagtac ccgggcaatg
     7441 cccaattccg caagattatt gagtgtatat aaagggttag cgcacatatc gaaggcgcga
     7501 tgaaaagtca ctgccagcgg accggcagca gccattattt tttccattcg tggcatatcg
     7561 acattcccgt caacatcgag aacgcccgtc accagtccag gaaaacctaa ttcgcggacc
     7621 gtgcgcacat cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg
     7681 cgtgggcgaa ttatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtacc
     7741 cccaacgacg gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg
     7801 ccgttttgct gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc
     7861 atttttgctc cttaattacg ctgactgctc gctggcaacg atctcttcaa tggaccacgg
     7921 atgaaactta atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc
     7981 atgctcacct tttggtgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc
     8041 agaaagcaat gccagcgcac gggcattcag cgtttctgga tcacccagca gaacgatttc
     8101 aatatgttcc caaccttcgt gtgggtaacg tttttccccg ggccacggta gctccacaat
     8161 agaaaactgc cagtgcgcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct
     8221 gccattgatc atattttctg acaaaagctc gccacactgt tcaaacccgc gacgccagcg
     8281 ttcagcagtg gcgttttgat ggcaacgcaa agaaatgtga tcggcagtca gcggagtgat
     8341 attcaacccc agacggaggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga
     8401 tgcaatatcc tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc
     8461 agtaaaagcg ttaatttacc ctgttgccct gcgccaacca accgctgatt tcacgccgct
     8521 tctgatgcaa tagtgaaaac ggcaatacgc cacgcgcacg ttgctgacga aaacagccat
     8581 ttgcagtata ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat
     8641 caatgtaagg tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca
     8701 tgattgcggc aggcgcgcct gcggattgcg aaccgcaggt tcgtcattca gcaaaagttc
     8761 agttcggcga ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatgacac
     8821 cgcgacaatt agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag
     8881 ttgagatcgc cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac
     8941 atgttcagca ggcgctggcg tccgatcgtc tcggtgtttc tacgccagaa aaacagacca
     9001 ttgtggttga ctactctgcg ccaaacgtgg cgaaagagat gcatgtcggt cacctgcgct
     9061 ctaccattat tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc
     9121 gcgcaaacca cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa
     9181 agcagcagca ggaaaacgcc ggtgatgaaa tggagctggc tgaccttgaa ggtttctacc
     9241 gcgatgcgaa aaagcattac gacgaagatg aagagtttgc cgagcgtgca cgtaactacg
     9301 tggtaaaact gcaaagcggt gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca
     9361 tcaccatgac gcagaaccag atcacctacg atcgtctcaa cgtgacgctg acccgtgatg
     9421 acgtgatggg cgaaagcctc tacaacccaa tgctgccagg aattgtggcg gatctcaaag
     9481 ccaaaggtct ggcagtagaa agcgaagggg cgaccgtcgt attccttgat gagtttaaaa
     9541 acaaggaagg cgaaccgatg ggcgtgatca ttcagaagaa agatggcggc tatctctaca
     9601 ccaccactga tatcgcctgt gcgaaatatc gttatgaaac actgcatgcc gatcgcgtgc
     9661 tgtattacat cgactcccgt cagcatcaac acctgatgca ggcatgggcg atcgtccgta
     9721 aagcaggcta tgtaccggaa tccgtaccgc tggaacacca catgttcggc atgatgctgg
     9781 gtaaagacgg caaaccgttc aaaacccgcg cgggtggtac agtgaaactg gccgatctgc
     9841 tggatgaagc cctggaacgt gcacgccgtc tggtggcaga aaagaacccg gatatgccag
     9901 ctgacgagct ggaaaaactg gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc
     9961 tctccaaaaa ccgcaccacg gactacatct tcgactggga caacatgctg gcgtttgagg
    10021 gtaataccgc gccatacatg cagtatgctt acacgcgtgt attgtccgtg ttccgtaagg
    10081 cagaaattaa cgaagagcaa ctggctgcag ctccggtaat catccgtgaa gatcgtgaag
    10141 cgcaactggc agctcgcctg ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag
    10201 gcacgccgca tgtgatgtgt gcttacctgt acgatctggc tggtctgttc tctggcttct
    10261 acgagcactg cccgatcctc agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac
    10321 tggcacaact gacagcgaag acgctaaagc tgggtctgga tacgctgggt attgagacag
    10381 tagagcgtat gtaatcgatt tttcgtgaga gtaaagcctg atcagggtta cctgatcagg
    10441 cttttttatt gccatctaaa tgtattctga aaatggacat gccattgttt tctgactgtt
    10501 ggataagagg ccagaagcgt aatatccggc cccagggaaa cgataacggt tgaatttaag
    10561 gaataccgca gtgtttaaat ttcttgtatt aacattaggc attatctctt gccaggctta
    10621 cgcagaagat acggttatag aaaacgacca tgacatttca gccatcaaag attgttggca
    10681 aaaaaattca gatgatgata ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata
    10741 caatctcgtc gatgcgcaat taaataaagc ctatggtgaa gcttatcgtt atatagaaca
    10801 agtgccacgc acaggtgtaa aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc
    10861 acagcgagcc tggctggatt ttagggacaa agaatgtgaa ttaatccttt caaatgagga
    10921 cgttcaggat ttaagtgacc cttattctga atcagaatgg ctctcatgta tgatcataca
    10981 gaccaatacg cgaactcgcc agttgcagct ataccgtaac tctgaagatt tttatccaag
    11041 ccctttgaca agaggataat tcacatcttt ttggcatgtt ttgttgcaag ctattcctga
    11101 taaataattg caacaagaca tcgagccttt ttcactgagt tattaaacat actcgcgagc
    11161 gcgtaatttt tttgtccttc agcgataatt cacaatcact tcattacgtt gtacctttag
    11221 cggtggaatt aaccgcccac cgcctggcac ttcccagata aatcgcaacg gttctgctgc
    11281 cgctataccg gaaaaggcca cggtggttcc gctctgcccc tctaattcga cacaacgaga
    11341 ttgcgaacac aagcgaaccc gcagtcctga aggtgtcggg ccgataagct gataacgcca
    11401 cgctaccagc gtcatcaatc ctgaagcagg ttgtcgcgta gaaagaggcg cagacgacat
    11461 cgactcaccg cgatgattta gcgtaattcc tacactactt gcctgccaca tcccctcccc
    11521 ggcggcttgc accagcagcg gaaacaataa tattgctaat aaggttctca ttatttgccg
    11581 ccaattgtcg ccgtcatgcg gatatgtcgg ttatcagaca gttccagatt cgacaggacc
    11641 actaactgcg gcaagctgcg gcgcaggaag cgagacaata atggtcgcag cgcgtggttc
    11701 accaacaata ctggcggcgc gcccagcatc tcctgacggg atagcgcttc ctgagtttgc
    11761 gccagtaaac gatccgccag ccctggctcc agtcctcccc cgccctgcag cgcctgtagt
    11821 aacaaacgtt ccagcggtgt atcgaggcca ataacatgga cttcatcttt gccaggaaac
    11881 cactgctggg taatcgcccg tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga
    11941 tcgctttgga tgggcgcatg ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc
    12001 ggtacttttt catcgaggag attttgcagc actttatgca gtgtggtgag cgtgacgacg
    12061 ccaggaacga gatcttccgt cagctttggc atctcctggg cgacgcgatc caacagctgt
    12121 tgcgcctcct gacgaccaaa cagctctgcg gcatgctggc taatgaggtg gttaagatgc
    12181 gttgctacca ccgtgctggc ctcaaccact gtgtacccct gaatctgcgc ctgttctttt
    12241 agcgcacttt caatccagat agcattcagg ccaaatgccg gatcgacggt cgcctcacca
    12301 ggtaacgtcc cggcagcggt tccagggtta atcgccagcc agcgccccgg ataagcatca
    12361 ccactgccaa tctccacgcc tttcatcaaa atgcgatagc gggcaggttg cagatccata
    12421 ttgtcgcgaa tgtgcaccac tggcggcaga aatcccatct cctgggcaaa tttcttgcgg
    12481 atactgcgta tacggcccaa caactcacca tcctgctgga aatcgaccat cgggatcagt
    12541 cgataaccca cttccattcc cagagaatct tccagttgta catcgttcca cgtcgcttcg
    12601 acaacggtat tattctctgc catttttacc ggtttgggtt cggcaggcgc tttttgttcg
    12661 cgtccgcgta tccaccaggc cagcccgagc aatccggcag tgaacagcaa aaataccagg
    12721 ttcggcattc caggcaccag gccgagtaaa ccgagcacgg cggcgcttaa caacataacg
    12781 cttgggttac tgaaaagctg attcaccatc tgctcgccaa catcctgatc ggtgctgaca
    12841 cgcgtaacga tgacccccgc ggcggtagaa atcaccagcg ccggaatttg tgccaccaga
    12901 ccgtcgccaa tggtcaatag cgtataactt tccgccgcgt gtcccatgct catgccatgt
    12961 tgcagcacgc cgaccagcaa cccgccgaca acgttaatga ccatgatgag gatcccggcg
    13021 atggcatcgc cgcgaacaaa cttacttgcc ccgtccattg agccgtaaaa atcggcttcc
    13081 tgagtcactt cggagcggcg ttttttcgcc tcatcttcac caatcaatcc agcgttaagg
    13141 tcggcgtcaa tcgccatctg cttacccggc ataccatcga gaacaaagcg cgcacccact
    13201 tctgcgatac gcccggcacc tttggtaatg accataaagt tgatgatcac gagaatgaca
    13261 aacaccacga taccgatagc gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc
    13321 accttccctg ccgccgccgc gccggtatgc ccttccatca aaatgatacg ggttgaagcc
    13381 acgttaagtg ccagacgcaa caacgtagta aacaacagaa tggtcggaaa cgcagcaaac
    13441 tcaagcgtgc gctgggtaaa catcgccacc agcaacacca tgatcgacaa ggcaatattg
    13501 aaggtaaaca acaggtcgag tatgaatgcg ggcagtggca gcaccatcat cgacaagatc
    13561 aacaggatca aaatcggtcc ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc
    13621 aggcgcagca tcgcggccag attactcatg ggtcggtttc tcgttaataa aatccagggc
    13681 ttccggcacc ggaagatgag taggttgtac agggcgctgt ccaccagcca gacgccagcg
    13741 tttcagttgc cagacccagg ccagcacttc cgccaccgcg gcgtacagtt gacccgggat
    13801 ttgttgacca atctccgcat gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt
    13861 cgggacgtta ttttcagcgc caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt
    13921 agcgaccact ttcggtgcgc tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg
    13981 ggtcgggtta ttgacaatga catcagcttt cggcacatcg gccatcatcc gacgccgtgc
    14041 agcagctcgc tgcatctgac ggatccgccc tttaacatga gggtcacctt cgctttgttt
    14101 gaactcatca cgaatatcct gccgcgacat acgcagcttt ttcaggtggc tgaagatttg
    14161 gaaaaagacg tcaaatccca ccattggaat gacaccaagc accaccagca gtgcgcatag
    14221 cccaaccaga tccatcgcat tacccatggc ggtagtcgga gactcggcca ttaagcgcat
    14281 catctgcggc cagtgatgcc agagataaaa ccccgtcacg ctgccaacca ggatggtttt
    14341 caaaattgcc ttaagcaact ccgcgccagt ctgtgccgag aacatccgtt taatgcccgg
    14401 tagcgggttg agtttggaaa acttcggctg caaggatttg ccgctaaata ccagcccccc
    14461 cagcatgacc ggagaaatga gcgccaccag caccacgccg ctaatcagcg gcagcagcgc
    14521 cagcatggct tctctgatca gcagaataat ctgcccgagg atcagattcg ggtca
//
LOCUS       DAZRVY010000100        14559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZRVY010000100 DAZRVY010000000
VERSION     DAZRVY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.100"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            71..427
                     /locus_tag="ACHXOX_003068"
     CDS             71..427
                     /locus_tag="ACHXOX_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7006471.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHXOX_003069"
                     /pseudo
     CDS             complement(478..1370)
                     /gene="yhaJ"
                     /locus_tag="ACHXOX_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1475..2176
                     /locus_tag="ACHXOX_003070"
     CDS             1475..2176
                     /locus_tag="ACHXOX_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR7006472.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHXOX_003071"
     CDS             2199..2363
                     /gene="yhaL"
                     /locus_tag="ACHXOX_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR7006473.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2497..3804)
                     /gene="cyuA"
                     /locus_tag="ACHXOX_003072"
     CDS             complement(2497..3804)
                     /gene="cyuA"
                     /locus_tag="ACHXOX_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR7006474.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAVAAAELE
                     GPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQA
                     IADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNGV
                     VFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEGL
                     SGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQG
                     ITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAAG
                     MAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDTA
                     VTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3832..5163)
                     /locus_tag="ACHXOX_003073"
     CDS             complement(3832..5163)
                     /locus_tag="ACHXOX_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7006475.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5438..6802)
                     /gene="tdcG"
                     /locus_tag="ACHXOX_003074"
     CDS             complement(5438..6802)
                     /gene="tdcG"
                     /locus_tag="ACHXOX_003074"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR7006476.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6874..7263)
                     /locus_tag="ACHXOX_003075"
     CDS             complement(6874..7263)
                     /locus_tag="ACHXOX_003075"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR7006477.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7277..9571)
                     /gene="tdcE"
                     /locus_tag="ACHXOX_003076"
     CDS             complement(7277..9571)
                     /gene="tdcE"
                     /locus_tag="ACHXOX_003076"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR7006478.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9605..10813)
                     /gene="tdcD"
                     /locus_tag="ACHXOX_003077"
     CDS             complement(9605..10813)
                     /gene="tdcD"
                     /locus_tag="ACHXOX_003077"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR7006479.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10839..12170)
                     /gene="tdcC"
                     /locus_tag="ACHXOX_003078"
     CDS             complement(10839..12170)
                     /gene="tdcC"
                     /locus_tag="ACHXOX_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR7006480.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12192..13181)
                     /gene="tdcB"
                     /locus_tag="ACHXOX_003079"
     CDS             complement(12192..13181)
                     /gene="tdcB"
                     /locus_tag="ACHXOX_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR7006481.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13280..14218)
                     /gene="tdcA"
                     /locus_tag="ACHXOX_003080"
     CDS             complement(13280..14218)
                     /gene="tdcA"
                     /locus_tag="ACHXOX_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR7006482.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3794 a         3877 c         3380 g         3508 t
ORIGIN      
        1 aaagatacga atagcattta cacaataccc ggaatagact ttcgtcactt ttaattaaag
       61 ggatgttttt atgcagtggt atttatccgt attaaaaaat tatgttggtt tctctggtcg
      121 cgcacgtcgt aaagagtact ggatgtttac tctgattaac gccatcgtcg gcgctattat
      181 caatgtcatt caattgattt taggtctgga gcttccatat ctgtctatgc tttatttgct
      241 ggcaaccttc cttcccgtcc ttgcgcttgc tatccgtcgc ttacacgata ccgaccgttc
      301 aggtgcctgg gccttgctct tttttgttcc gtttattggc tggctcgtgc ttctggtttt
      361 tttctgcaca gaaggtactt ctggcagcaa tcgttacgga aacgatccga agtttggttc
      421 aaattaatct tagaattggg gcgatatttc gccccttttt attaacaata ataaatatta
      481 ttttccgcta aaaagtttgg gaatttcccg cagacaccag gatttcgctt cccccatact
      541 gtcacgacgc caggccataa taatatcgat ctcactggtc gattccgggc tgacgacacg
      601 caaccgccct tccgcaatat ctttttcgac catcggatac ggcatcgtcg ccacgccaag
      661 ccccgccagt aatgcctgac gtttatcttc aatcgtgctc accgttaagc gcggctgttt
      721 gtccagcagt tgtacggtca acaccgggcg ctcacgagcg gtatccgcta ccgcaattcc
      781 acgatatttc acgcgcgtca cttcagataa cggttccggc tcctgatgaa tcgggtgatc
      841 aggcgcggca acgtagacgt tcattaacgt atagagcttg cgcgagttga tctccgacga
      901 ggaacgaaaa tgcatatccg gcgcgataac aatatccgcc cgcccctgct ccagtcgttc
      961 ctacgccccc gccagcactt cggtgatgat tgccagttgg gtattggctt ttgccgccag
     1021 tttgtcgatt aacgggaaaa aggcaggtgt cggtaccagc gcttcggtca caatggtgag
     1081 atgcgtttcc caaccgcgcg cgagggcttc cgcatcggta gtcagtttat ctgcggcttc
     1141 cagcaaaacg cgccctcgct ccagcaacat cctcccgaca ttggtgaatt tggtgcgatg
     1201 gcccgagcgg tcaaacagca ccacatccag ctcttcttcc agtttttgca tggtgtagct
     1261 aagtgcggaa ggcacgcgtc ccagctcatc cgccgccgcc gcaaaactgc cccggcgatc
     1321 gcatccataa cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca
     1381 ttcaggaaat ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct
     1441 taccataaaa ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca
     1501 gtgtggacaa gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta
     1561 cttcgacccg aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc
     1621 cccaggtgcc gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct
     1681 ggatggagaa gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga
     1741 ggcgttgctg ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga
     1801 caaaccgtta acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct
     1861 gattcaaaaa ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac
     1921 gatgggaagc ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg
     1981 cgaaagtgcg aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa
     2041 atttcatgcg cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt
     2101 tattcgtgac gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat
     2161 agatttgcct gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa
     2221 agcgccagcc agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg
     2281 gctatgaaga gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag
     2341 ccgaggatga agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg
     2401 gcctacaaaa tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc
     2461 agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat
     2521 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2581 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2641 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     2701 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2761 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2821 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     2881 tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg
     2941 ttctgctaca accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt
     3001 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     3061 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     3121 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     3181 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     3241 gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc
     3301 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     3361 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     3421 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3481 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3541 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3601 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3661 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc agcgccagtg aaatcggttc
     3721 agtacatccc agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa
     3781 cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa
     3841 tgccaggaac ggagaaacac aaagcaacaa accagtgacg ataatcaggt acagagacat
     3901 ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc cacgccggga tcaggcaccc
     3961 taccatgccg aaaatcgggc tacagataga ggtgaagctc aacaccggtg cgttcagtac
     4021 gatagcgctc caggccagca aaatggcgaa aatcatgatg ccgcgctgaa caagattttc
     4081 gttaatcttc tcggcaggca tcttgcgacg caggatgttc attacgatcc cttgcgttgc
     4141 ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg acagcaaaga tattgagaat
     4201 gacgctgacc actttcaccc acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc
     4261 cagcgcggaa atattctgct cgtaggcttt taccgcttcg tcatgcccca tcgccagcgt
     4321 gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg ccaaacgcga tattcattgc
     4381 ccgcaatgct ttatgccgcg ccacttcaat agatttttcc cgcgagcgat aagagatcac
     4441 catcgggctt aacgtctgga taaacaaaat cgacgtcagg gtaaacggca gcgtaataat
     4501 ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg acgttataca gatgccacat
     4561 tcccaccatc gacacgccca gcgccgcgac caccagcagc ttggtcagca ccatgccggt
     4621 cgaaattttg aataacaatt tctcgccgcg tgaagagatc gccaccagaa tgcaaatcag
     4681 taccagacca taaaagggac tgtctgacag caacccttcc gtcacgccga aggtatgcag
     4741 ataggaagca ctatcgttgg tgatggcggt ggaatagacg aacatccaaa tcaccagcat
     4801 tacgaaatag aacgcaccta acaggatgcc ccagttttta cctaaataac cgctaatgac
     4861 actcgggtaa tctttacatt ctggtgattc tgccagcgta ttaataaaca accgctgaaa
     4921 cagatacatt gccgggtaac caatcaccga tgagagcaaa aatacccaca atcccatcaa
     4981 accgacctgc accgggagaa aaacaatccc cgcgccaatc gccatcccga tactcataat
     5041 cacccagccg gtgtcagtac tgtcgaattt gatcgcttct cgccactcgc tctcactcat
     5101 tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc
     5161 cataatttct cgctcaatat tttgtagggc ttattatttt tttccgagcc gcatcaaggc
     5221 gatatgcggt ctacgttttt gcaggcgagt tattagaata aagaaatgat acgcgcgagg
     5281 tcagagaaaa tcttcacaat caagactttg aaataacgga gatggataag aattttctac
     5341 taaattaatc gcaagagaat attcattctc tatttgcggc agatcacaaa aaaaaggtgc
     5401 acatttgtgc acccaaggat gaaagctgac agcaatgtca gccgcagacc actttaatgg
     5461 ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat atctttgccg gtttcataca
     5521 tcgtctcgat cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc
     5581 acgcggcgtt tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta
     5641 cctgtccggc aaccggatcg caggtcagcc caaggttatg ctccatcgcg atttccgccg
     5701 cattgcatac ctgcgccgga ctgccgccca gtagttcagt taaccctgcc gccgccattg
     5761 aacaggccac gccaatctcc ccctgacagc cgacctccgc gccagagatg gaggcgttca
     5821 ttttatacag cgcgccaata gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt
     5881 ttaccggacg acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac
     5941 cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg
     6001 cgtacatgtt gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctagaaa
     6061 ccagctgacg acgcagcgct acggcacgac gcggcacatt gagcggacca ggcagcacgc
     6121 cttcagtgtt catcccgcgt tcaataccgt catgcatcac ttgccagata cgggcaaatc
     6181 ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt gtgcatcatc aggccagata
     6241 tagacaggcc gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag
     6301 gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc
     6361 cgccgacaga gtaataggtt ttacttaata gcgcttcctg tcccttccag gcagtgatcc
     6421 gcattccgtt ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag
     6481 gaaaatcaac aatatgcgca ccagatgcca ctggcagccg cccgctgcgc gttactaact
     6541 ctataaatgc agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca
     6601 tgataatggc gacatccgtg gcatggcctt tgcccgtcag tgacaacgac ccatacagat
     6661 cgaccacaat atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac
     6721 tttttccggc attcattggc cccacggtat gcgaactgga gggaccaatc ccaattttga
     6781 aaatatcgaa tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg
     6841 gaacgacctt gcgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatagcttc
     6901 aatttccagc ttcacatctt tcggcaaacg cgcaacctgc acacagctcc gggtcgggta
     6961 ggtcgcctga tgctcatcga agaactgctt atagacttca ttgatggtgg caaaatcatt
     7021 cagatcggtg atgaacacgg tcatcttgat gatatcgccc acgctcagcc cggcagcaac
     7081 cacgatcgct ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat
     7141 ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg aagaccatgc tgcctaaatc
     7201 aacgccctga acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt
     7261 catacatcct ccggcgtcag agcgcctggg taaaagtacg tgaaataaca tcctgttgct
     7321 gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag
     7381 gatatttttc cgggtgctcg atggcatcca gcagcatttc ccgattcatt acgttgacgt
     7441 tgaggtgttg accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga
     7501 caaggttggt tttacgtact ggatcttctt tgcccagcgc cgcaggaacg attgagaagg
     7561 tgtacgagat cccatctttg gcgtaggtga acggcagttt cgccaccgac gtcaatgagg
     7621 ccacggcacc tttgcggtca cgaccatgca tcgggttagc gcccggcgcg aacggtgttc
     7681 cggcgcgacg accgtccggc gtattaccgg ttttctggcc gtacaccacg ttagaagtga
     7741 tagtcagaat cgactgggta gggacggcgt tgcgataggt tggcagcgct ttaattttct
     7801 tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt
     7861 actgcggata ttcaccgtcg atttcaaagt ccaccgccag gccgttttcg tcacggattg
     7921 gtttcacgcg ggcatatttg atggcagaca gggagtccgt cgccaccgac aggcccgcga
     7981 tgccgcatgc catagtgcga tagacatcac gatcgtgcag cgccatcagc gaagcttcgt
     8041 agctgtactt gtcgtgcatg tagtgaatga tattcagcgc gctgatgtac tgcaccgcca
     8101 gccagtccat gaagtgatcg aggctgtcca tcactttgtc gtaatccagc acgtcgtcca
     8161 tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa
     8221 ttgcgtacag cagtgttttc gccaggttag cgcgtgcacc aaagaactgc atttgcttac
     8281 caatcaccat tgggctgacg caacaagcaa ttgcgtaatc gtcgctgttg aagtcagtac
     8341 gcatcagatc gtcgttttca tactgcaagg aagaggtgac gatcgacacc tgcgcggcat
     8401 attttttgaa ggcaatcggt aattcttccg accaaagaat ggtcaggtta ggttccggtg
     8461 ccggccccat agtgtgcagg gtgtgcaaat agcggaagga gtttttggtc accagcgtac
     8521 gaccgtccag ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca
     8581 gcgaatcaaa ttccggcgta cgcaagaagc gcaccatacg gatcttcata atgaagtgat
     8641 cgatcagttc ctgtgcctgc tgctcattga gtacgcctgc tttaaagtcg cgctcaatgt
     8701 agatatcgag gaacgacgcc gtgcgcccca gcgacatcgc gccgccgttc tgtgatttca
     8761 ctgccgccag ataagcgaag tagagccact gcaccgcttc ctgcgcattc tgcgccgggc
     8821 gagagatatc aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat
     8881 gctctgccag ctcctcacgc agacggatgg tggcttccag atcttcgcct ttttccagac
     8941 gggactggag atcggcaaat tgcagttcgc gttcacgcac cagataactg atgccataca
     9001 gcgctacgcg gcgatagtca ccgataatgc gcccacggcc atagccatct ggtaaaccgg
     9061 tcagcacgcc agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct
     9121 ggttatgggt tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc
     9181 cataggcgtg gaatgaactt ttaatcatat taatgccacc gaacggatgc agcgcgcgct
     9241 ttaatggcgc atccgtttgc agaccaacaa ttttttccag cggctggtta atatatcccg
     9301 catcatgagc ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg
     9361 cgttttcgat acggatgcct tccattactt tttcccacaa ttccgtggtg gcaggcgtcg
     9421 cttcggcgag gaaagattca tcgccttcat acggtgtata gttatgttga ataaaatcgc
     9481 ggacattaat ttcgtttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca
     9541 gcttatcgct ggtatcaata tctatcttca tgaaaaataa tctctctaca atacttcaac
     9601 taaattatgc aaattctgcg ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa
     9661 tcattttttc ttcgttagtc ggaataacgg cacaaatgac acgcgcattt tcactggaaa
     9721 caattcgctc tccaaaagag ttagagcgat tattcatttc tgtatcaatc actacgccta
     9781 atacagccaa atgttccatg accagacgac gaattaaact tgaattctct cctattccgc
     9841 cggtgaatat aattccatcc aggcgacgta atgaagctgc gtgtccggca atatgacggg
     9901 caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg
     9961 ctttttccag aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt
    10021 tattcactac gcgttccagg tcacccaggc tctggttggt ttggctggcg acccaggaca
    10081 tcgcaccaaa gtcgacatcg ccactgcggg ttcccatcat caagccttcc agcggcgtca
    10141 ttcccattga agtatcaaca ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc
    10201 caagatgcgc cacaaccagg ccggagtcat cttccgccag attcagcaga gaatgtgcgc
    10261 gctgggaaac atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt
    10321 cataatattt ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac
    10381 tggtatcaaa taccgccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac
    10441 cacttaaatt ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa
    10501 tgacttcatc ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt
    10561 ggccaattaa ggccacactg tcatttaaat tccgtttttc cagttcaaat gcaattgcct
    10621 tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata
    10681 agaatgcatt ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc
    10741 tggcatcgag cacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg
    10801 gaaattcatt catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg
    10861 ttcaggatgg tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat
    10921 ttcgccagag acggcgcttt acggatggca tacatcggca acaggcacag cagggatgcg
    10981 ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt tcgggttggc gtaggcaaca
    11041 acccaggtgg agcccatgat gaagatcatg ctgatagtgt tcagtttacc cagcgacact
    11101 ttagttttgt cgcctttata accaaacttc aggaccaggc cattcagacc ttccagcgtt
    11161 cccagatagt gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca
    11221 tattccagtg taatcgcgaa cgttgttttg gtaccggtca tggacgcaaa gtggttagcc
    11281 agataagaaa gcactggaat attctgcgct ttggcttccg ccatgttggc cggagacagc
    11341 gtaaataggc agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga
    11401 gaaatgattt gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct
    11461 tcacgtttag aaaccacgaa ggaagagacg attggcgaga aattaaagga gaaaaccatg
    11521 atggaaatcc ccagccagac ggtgatcagg ataccgtcat gaccggttaa cgacagcgaa
    11581 ccgaggtcaa cctggtcgat aactgcagag ttccagtaag ggatcagcga cagagaaatc
    11641 agcaccaggc tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc
    11701 ttaccaaacc agatgacgaa agccatcagc agcagcagga acagcgccac aaagccgcga
    11761 ttcagcggtg caaagccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta
    11821 acgccataaa tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg
    11881 ccgccagttt taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag
    11941 ccagaaagac atagacgcgc cagcgcccgg tggcaataaa atgcgatggg gtatgccaat
    12001 accaacatca gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc
    12061 accccggcgc cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc
    12121 caggacgatt gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct
    12181 caacgaatta attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata
    12241 ttgccgccgg aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg
    12301 cttaataatg cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga
    12361 attaaggcaa tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact
    12421 aattcacgaa cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc
    12481 agggtgccgg tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg
    12541 tgaacgtttt cagactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc
    12601 accgcaatac cagcaattaa accgccacca ccaattggca caatcacgtt atcgacatca
    12661 tagagatctt ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca
    12721 tcgtaaggtg ggataaaaat acggccttcc atttcgacaa tttcgctcac tttagcgata
    12781 gtgtcgttga agttatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct
    12841 actttggatt ttggcgcacc ttttggcatc accactttac cgtcgatacc cagcatcgcg
    12901 caggagagcg aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt
    12961 ttttccgcat cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc
    13021 gtacgctgca tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag
    13081 ttggagcgag gcatgcctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata
    13141 atatcatcaa tagcaatcgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg
    13201 aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg
    13261 tttaaataaa aacaaataac taaccaactt ctattaattg ccttcgtcta cacccattat
    13321 aagatgaata ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat
    13381 ttttcgacca taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa
    13441 taaattgatt agaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag
    13501 cattgagaac aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac
    13561 tgatgccatt tctttgtaac gtagtaagca gttcgctgta gtaccccata ttagtttgtg
    13621 gcaacaccca ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg
    13681 acttactggc taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa
    13741 gcttcatttc tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg
    13801 gtaagaacga agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt
    13861 tgaacttgtt gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg
    13921 agacttccac caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt
    13981 cacgggtaat ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac
    14041 cggtattttt ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt
    14101 tactgacggc cggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc
    14161 taatgacttc ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa
    14221 cgacctacgt taattacctc attgacggca tgaagtgtat caaaatgaaa tgaacaggat
    14281 atgtgcgacc actcacaaat taactttcaa tactttccag ggtatcgtca ttacaattaa
    14341 atacctttaa atatcaacaa gttaaagcat aaaaatcagc ataaaccctg atttttgtca
    14401 aaaaaaatga cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat
    14461 acaaaaaagg ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt
    14521 gagttaaatt taatgagcaa gttctcaaat tttattata
//
LOCUS       DAZRVY010000101        14297 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZRVY010000101 DAZRVY010000000
VERSION     DAZRVY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.101"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHXOX_003081"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHXOX_003081"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR7006483.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHXOX_003082"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHXOX_003082"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR7006484.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHXOX_003083"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHXOX_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR7006485.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHXOX_003084"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHXOX_003084"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR7006486.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHXOX_003085"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHXOX_003085"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR7006487.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHXOX_003086"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHXOX_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR7006488.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHXOX_003087"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHXOX_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR7006489.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHXOX_003088"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHXOX_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR7006490.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHXOX_003089"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHXOX_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR7006491.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHXOX_003090"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHXOX_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR7006492.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZRVY010000102        14180 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZRVY010000102 DAZRVY010000000
VERSION     DAZRVY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.102"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="ACHXOX_003091"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="ACHXOX_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR7006493.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="ACHXOX_003092"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="ACHXOX_003092"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR7006494.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="ACHXOX_003093"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="ACHXOX_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR7006495.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="ACHXOX_003094"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="ACHXOX_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR7006496.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="ACHXOX_003095"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="ACHXOX_003095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR7006497.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="ACHXOX_003096"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="ACHXOX_003096"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR7006498.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="ACHXOX_003097"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="ACHXOX_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR7006499.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="ACHXOX_003098"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="ACHXOX_003098"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR7006500.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="ACHXOX_003099"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="ACHXOX_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR7006501.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="ACHXOX_003100"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="ACHXOX_003100"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR7006502.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /gene="sirB2"
                     /locus_tag="ACHXOX_003101"
     CDS             11095..11487
                     /gene="sirB2"
                     /locus_tag="ACHXOX_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR7006503.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12300
                     /gene="sirB1"
                     /locus_tag="ACHXOX_003102"
     CDS             11491..12300
                     /gene="sirB1"
                     /locus_tag="ACHXOX_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR7006504.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12336..13190
                     /gene="kdsA"
                     /locus_tag="ACHXOX_003103"
     CDS             12336..13190
                     /gene="kdsA"
                     /locus_tag="ACHXOX_003103"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR7006505.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13339..13446)
                     /locus_tag="ACHXOX_003104"
     CDS             complement(13339..13446)
                     /locus_tag="ACHXOX_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7006506.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13874..13981)
                     /locus_tag="ACHXOX_003105"
     CDS             complement(13874..13981)
                     /locus_tag="ACHXOX_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7006507.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3538 a         3543 c         3618 g         3481 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaatct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggcaggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg ttttattatg ggtcgcgaat
    11821 cgtctcgatt tgccgctgct gccggtgatt ttccctacgc agctgatatt gcgcattgaa
    11881 tgtccggatg gcgaaatttg gctgattaat ccttttaacg gtgaatcgtt aagcgaacat
    11941 atgctggacg tatggttaaa gggaaatatc agcccgtcgg cggaactgtt ttatgaagac
    12001 cttgatgaag ctgataacat tgaggtaatc cgcaaattgt tggatacact caaagcctcg
    12061 ttgatggaag aaaatcagat ggagctggcg ttacgcacca gcgaagcttt attacaattc
    12121 aacccggaag atccctatga aattcgcgat cgcgggttga tttatgcgca actggattgc
    12181 gaacacgttg cgttgaacga tttaagttat ttcgttgaac agtgtccgga agacccgatc
    12241 agcgaaatga tccgtgcgca aataaataac atcgcgcata aacatattgt gctgcattaa
    12301 ttaatcgaca ttttactcaa gattaaggcg atcctatgaa acaaaaagtg gttagcattg
    12361 gcgacatcaa cgtagcaaat gacctgtcgt tcgtactgtt tggtggtatg aacgtgctgg
    12421 aatctcgcga tctggcgatg cgcatttgcg agcactacgt aaccgtgacc cagaaactgg
    12481 gtatccctta cgtgttcaaa gcctcttttg acaaagccaa ccgctcctcc atccactctt
    12541 atcgtggacc gggcctggaa gaagggatga aaatcttcca ggagttgaag cagacttttg
    12601 gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc acagcccgtt gctgatgtcg
    12661 tggatgtgat tcagttgccg gcgtttcttg ctcgccagac tgacctggtt gaagccatgg
    12721 cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt tgtcagcccg ggacagatgg
    12781 gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa agtgattctt tgcgatcgcg
    12841 gtgctaactt cggctatgac aacctggttg tcgatatgct gggcttcagc attatgaaga
    12901 aagtgtctgg taactcgccg gtgattttcg acgtgaccca cgcactgcaa tgccgcgatc
    12961 cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc tgagctggca cgagccggta
    13021 tggcggtagg tctggcgggg ctgtttattg aagcgcatcc ggatccggaa catgcgaaat
    13081 gtgatggtcc atccgcgctg ccgctggcta aactggaacc gttcctcaag cagatgaaag
    13141 cgattgatga tctggtgaaa ggtttcgaag aactggatac cagcaagtaa tcttttttgc
    13201 ttgaaaaata aagtcttagc gttctgcgtt aagacttttt tcatgggtgc cagatacaaa
    13261 aaaggccgca ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc
    13321 ctgacgcccg ttttcacctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat
    13381 aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag
    13441 cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga attgttctgg
    13501 ttcaagatta gcccccgttc tgttgtcagg ttaaacctct caacgtgcgg gggttttctc
    13561 tttccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggataa
    13621 cgccggcttg atgtggatac tacgtctcaa ttcatcttca cttcatccct gaaatgtttg
    13681 caatgaagag tgcattccgg tttttcaaca gctgttacag tcgtttcatg agtgctctgg
    13741 atgatgcttc cagctcgggt tgccaatatt tacttgtgga ggagataaag acaaaaatgg
    13801 ccgcaggctg ttacccctgc ggccggtttc gggcgcatat tgccatcacg gcagcctgac
    13861 gcccgttttc accttacttc cggttacgcc accagccgac aatcgctgcg gtaataatcc
    13921 ccgccaggat cggtgctgcc aggtcgtgcc agaaaatcat ggcaaacttc gcgagcgtca
    13981 tatagccgcc ttgttgtaat gacaacattt tgcggctatt cttgaattgt tgatggttca
    14041 agattagccc ccgttctgtt gtcaggtttt acctctcaac gtgcgggggt tttctctttc
    14101 cagcaaccaa tgccactagg gattaagccc ccgcaacatt gcgcctcacc ggataacgcc
    14161 ggcttggtgt ggatactacg
//
LOCUS       DAZRVY010000103        13964 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZRVY010000103 DAZRVY010000000
VERSION     DAZRVY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.103"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXOX_003106"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXOX_003106"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR7006508.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHXOX_003107"
     CDS             2273..2662
                     /locus_tag="ACHXOX_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7006509.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXOX_003108"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXOX_003108"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR7006510.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXOX_003109"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXOX_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR7006511.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHXOX_003110"
     CDS             complement(4321..4521)
                     /locus_tag="ACHXOX_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR7006512.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHXOX_003111"
     CDS             4687..5232
                     /locus_tag="ACHXOX_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR7006513.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXOX_003112"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXOX_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXOX_003113"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXOX_003113"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR7006514.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHXOX_003114"
     CDS             complement(6529..7353)
                     /locus_tag="ACHXOX_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR7006515.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXOX_003115"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXOX_003115"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR7006516.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXOX_003116"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXOX_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR7006517.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXOX_003117"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXOX_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR7006518.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXOX_003118"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXOX_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR7006519.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHXOX_003119"
     CDS             complement(11316..11969)
                     /locus_tag="ACHXOX_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR7006520.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXOX_003120"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXOX_003120"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR7006521.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXOX_003121"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXOX_003121"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR7006522.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3536 c         3712 g         3326 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAZRVY010000104        13902 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZRVY010000104 DAZRVY010000000
VERSION     DAZRVY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.104"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="ACHXOX_003122"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="ACHXOX_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR7006523.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="ACHXOX_003123"
     CDS             complement(1549..1722)
                     /locus_tag="ACHXOX_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR7006524.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="ACHXOX_003124"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="ACHXOX_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR7006525.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="ACHXOX_003125"
     CDS             3360..3689
                     /locus_tag="ACHXOX_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR7006526.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHXOX_003126"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHXOX_003126"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR7006527.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHXOX_003127"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHXOX_003127"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR7006528.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHXOX_003128"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHXOX_003128"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR7006529.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHXOX_003129"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHXOX_003129"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR7006530.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHXOX_003130"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHXOX_003130"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR7006531.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHXOX_003131"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHXOX_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR7006532.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="ACHXOX_003132"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="ACHXOX_003132"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR7006533.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3655 a         3436 c         3232 g         3579 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgagag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tc
//
LOCUS       DAZRVY010000105        13901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZRVY010000105 DAZRVY010000000
VERSION     DAZRVY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.105"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(9..173)
                     /gene="yojO"
                     /locus_tag="ACHXOX_003133"
     CDS             complement(9..173)
                     /gene="yojO"
                     /locus_tag="ACHXOX_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR7006534.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            282..776
                     /gene="napF"
                     /locus_tag="ACHXOX_003134"
     CDS             282..776
                     /gene="napF"
                     /locus_tag="ACHXOX_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR7006535.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            766..1029
                     /gene="napD"
                     /locus_tag="ACHXOX_003135"
     CDS             766..1029
                     /gene="napD"
                     /locus_tag="ACHXOX_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR7006536.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            1026..3512
                     /gene="napA"
                     /locus_tag="ACHXOX_003136"
     CDS             1026..3512
                     /gene="napA"
                     /locus_tag="ACHXOX_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR7006537.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3519..4214
                     /gene="napG"
                     /locus_tag="ACHXOX_003137"
     CDS             3519..4214
                     /gene="napG"
                     /locus_tag="ACHXOX_003137"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR7006538.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4201..5064
                     /gene="napH"
                     /locus_tag="ACHXOX_003138"
     CDS             4201..5064
                     /gene="napH"
                     /locus_tag="ACHXOX_003138"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR7006539.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5061..5510
                     /gene="napB"
                     /locus_tag="ACHXOX_003139"
     CDS             5061..5510
                     /gene="napB"
                     /locus_tag="ACHXOX_003139"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR7006540.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5520..6122
                     /gene="napC"
                     /locus_tag="ACHXOX_003140"
     CDS             5520..6122
                     /gene="napC"
                     /locus_tag="ACHXOX_003140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR7006541.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6135..6758
                     /gene="ccmA"
                     /locus_tag="ACHXOX_003141"
     CDS             6135..6758
                     /gene="ccmA"
                     /locus_tag="ACHXOX_003141"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR7006542.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6755..7417
                     /gene="ccmB"
                     /locus_tag="ACHXOX_003142"
     CDS             6755..7417
                     /gene="ccmB"
                     /locus_tag="ACHXOX_003142"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR7006543.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7459..8196
                     /gene="ccmC"
                     /locus_tag="ACHXOX_003143"
     CDS             7459..8196
                     /gene="ccmC"
                     /locus_tag="ACHXOX_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR7006544.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8193..8402
                     /gene="ccmD"
                     /locus_tag="ACHXOX_003144"
     CDS             8193..8402
                     /gene="ccmD"
                     /locus_tag="ACHXOX_003144"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR7006545.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8399..8878
                     /gene="ccmE"
                     /locus_tag="ACHXOX_003145"
     CDS             8399..8878
                     /gene="ccmE"
                     /locus_tag="ACHXOX_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR7006546.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8875..10818
                     /gene="ccmF"
                     /locus_tag="ACHXOX_003146"
     CDS             8875..10818
                     /gene="ccmF"
                     /locus_tag="ACHXOX_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR7006547.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10815..11372
                     /gene="dsbE"
                     /locus_tag="ACHXOX_003147"
     CDS             10815..11372
                     /gene="dsbE"
                     /locus_tag="ACHXOX_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR7006548.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11369..12421
                     /gene="ccmH"
                     /locus_tag="ACHXOX_003148"
     CDS             11369..12421
                     /gene="ccmH"
                     /locus_tag="ACHXOX_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR7006549.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12456..13103)
                     /gene="narP"
                     /locus_tag="ACHXOX_003149"
     CDS             complement(12456..13103)
                     /gene="narP"
                     /locus_tag="ACHXOX_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR7006550.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13423..13794
                     /locus_tag="ACHXOX_003150"
                     /pseudo
     CDS             13423..13794
                     /locus_tag="ACHXOX_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2984 a         3489 c         4070 g         3358 t
ORIGIN      
        1 ttttagtgtt aaattctgat gagggagcaa accagcagaa atatctctgg tgaattaatc
       61 gctatataaa tatgtttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
      121 tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg catctttgac
      181 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
      241 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
      301 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
      361 ccggtgatga atctcatttt ctgacccatt gcacccgttg cgacgcctgt attaatgctt
      421 gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata
      481 acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc
      541 gccacaccag ggcctgggat ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt
      601 cagttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
      661 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
      721 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
      781 caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
      841 ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
      901 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
      961 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
     1021 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
     1081 cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
     1141 ttaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa
     1201 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
     1261 tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac cgtttgacgc
     1321 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
     1381 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
     1441 ggccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
     1501 cgtccaagct gtttaaagcg ggcttccgtt cgaacaacat cgacccgaac gcgcgtcact
     1561 gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag ccgatgggct
     1621 gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgcaaac atggcggaga
     1681 tgcacccgat cctctggtcg cgcatcacta accgtcgtct ctctaatcag aacgtcaccg
     1741 tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
     1801 ttacgccaca gtctgacctg gtgatcctga actacatcgc caactatatc attcaaaaca
     1861 atgcgataaa tcaggacttc ttcagcaaac acgttaacct gcgcaaaggg gcgacggaca
     1921 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccaggttctg
     1981 acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgccgag tatacgctgg
     2041 aaaaaaccgc tgaaatgacc ggcgtgccga aagaccagtt agaacaactg gcgcagctgt
     2101 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcatactc
     2161 gtggcgtgtg ggctaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
     2221 agccgggttg cggtccgttc tccctgaccg ggcaaccttc tgcgtgtggt actgcgcgtg
     2281 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgaccaac gagaaacacc
     2341 gtgatatctg cgagaagaag tggaatatcc cgagcggcac cattccggcg aaaatcggtc
     2401 tgcacgcggt ggcacaagac cgtgcgctga aagacggcaa actgaatgtt tactggacca
     2461 tgtgtaccaa caacatgcag gccgggccga acatcaatga agagcgtatg ccgggctggc
     2521 gcgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt gcgctggccg
     2581 ccgacttgat cctgccgacc gcaatgtggg tagagaaaga gggtgcttac ggtaacgccg
     2641 aacgccgtac tcagttctgg cgtcagcagg tacaggcacc gggcgaagcg aaatcggatc
     2701 tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtgtgg ccggaagagc
     2761 tgctagcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagtgctt tatgccacac
     2821 cggaagtgag caaattcccg gtatccgaac tggcggaaga tcagctgaac gatgaatccc
     2881 gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg
     2941 gtcacggtca cgatctcgcg ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc
     3001 cggtggtgaa cggtaaagaa acgcagtggc gttacagcga aggtaacgac ccgtacgtga
     3061 aagcgggcga aggctataag ttctacggta aaccggatgg taaagcggtg atcttcgcgc
     3121 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
     3181 gacgcgttct ggagcactgg cacactggca gtatgactcg ccgtgtgccg gaactgcacc
     3241 gcgccttccc ggaagcggtc ttgtttattc acccgctgga tgcgaaagcg cgcgatctgc
     3301 gccgtggcga caaagtgaaa gtggtttctc gccgtggcga agtgatctcg attgttgaaa
     3361 cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat gccgttcttc gacgccgcac
     3421 agctggttaa caaactaacg ctggacgcga ccgatccgct ctcgaaagag acggacttca
     3481 agaagtgcgc agtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
     3541 aaaatggtcg ccgccgcttt ctgcgcgatg ttgttcgcac agcaggcggg ctggctgccg
     3601 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
     3661 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
     3721 ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcttctggc ctgtcggcgg
     3781 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
     3841 ccaaagtgtg cccgagtggt gcgctggatc gtgagatcga atcgatcgac gacgcgcgga
     3901 tggggctggc ggtgctggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
     3961 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
     4021 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
     4081 gctgcggtaa gtgcgaaaaa gtgtgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
     4141 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggctggctg gaggggaaca
     4201 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg tgaaaaaagg ctggtggcgc
     4261 agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
     4321 agcggtccgt ggtttggtgt gtggatctta cacggcaact acagctgtag cctgttattc
     4381 gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag tggtcatctg
     4441 cctgccacgg tggcgttgac cggtgcggtg ataatcactg tgctctacgc cctggcagga
     4501 aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt agcgaactgg
     4561 ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat tcgctacgtg
     4621 ctgttggtgg tcattctgct gggatcagcc ttaaccggca cgctcatctg ggaatggatc
     4681 aaccccgttt ccctgatggg gcgtagcctg gtaatgggct tcggcagcgg cgcgctgctt
     4741 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggcacatt
     4801 tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
     4861 agcgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
     4921 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
     4981 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
     5041 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
     5101 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
     5161 gccagtcgcc ggaagtatcc gggacgcagg aaggggccat tcggatgcca aaagagcagg
     5221 atcggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
     5281 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agctatcgca
     5341 ccactggcgc gccgcgtatc agtcctactc actttatgga cagcgacggc aaagtgggcg
     5401 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
     5461 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
     5521 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
     5581 ccagccgtct ggcgctggga acgctgctgt tgatcggttt tgtcggcggc atcgtcttct
     5641 ggggcggctt taacaccgga atggaaaaag ccaataccga agagttctgc attagctgcc
     5701 acgaaatgcg taacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
     5761 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccgaagatga
     5821 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
     5881 cgcagaaatt tgaagctcat cgtctgacaa tggcacagaa tgagtggcgg cgcatgaagg
     5941 acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
     6001 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
     6061 attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt
     6121 aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgtgag cgggatgaac
     6181 gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta caaatcaccg
     6241 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
     6301 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
     6361 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
     6421 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
     6481 ccgggcttgc cggattcgac gatattccgg taaatcaact ttctgccggg caacaacgcc
     6541 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc gacgagcctt
     6601 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg
     6661 agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct gaaagtaaaa
     6721 ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg ttctggcgca ttttccgtct
     6781 tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct
     6841 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcgcgtat
     6901 tgcaccgggc attatctggg ttgctgcgct gctttcatcc ttgctggcgc tggaacgact
     6961 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
     7021 gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaaccggtc tgccgttact
     7081 catcctttcg ccactggtag caatgctact gggaatggat gtttatggct ggcaagtgat
     7141 ggcgctgacg ctgctgctgg gaacgcctac gcttggcttt ctcggtgcac cgggtgtggc
     7201 gttgacagtg ggacttaagc gcggtggtgt gctgctcagc atactggtgt taccgctgac
     7261 tatcccatta ctcatctttg ccaccgccgc gatggacgcg gcttccatgc atttgcccgt
     7321 tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
     7381 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
     7441 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
     7501 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc
     7561 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
     7621 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
     7681 gcagtggcag cgtttattgg ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg
     7741 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
     7801 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
     7861 ctgctgtttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg
     7921 gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
     7981 tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
     8041 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc
     8101 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
     8161 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
     8221 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
     8281 ttccgctggt ggttttggtc gtgcactcgg tgatgcaaca tcgcgcaatt ctgcgtggtg
     8341 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
     8401 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgcc gtgttggcag ggctggcgct
     8461 gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt atacgccggg
     8521 ggaaattctc tacggcaagc gtgaaactca gcaaatgccg gaagtcggtc agcgtctgcg
     8581 cgttggcggg atggtgatgc tgggtagtgt gcagcgcgat cccaattcgc tgaaagtgac
     8641 cttcactatt tacgatgctg aaggctcagt ggatgtctct tacgaaggca ttttgccgga
     8701 tctgttccgt gaagggcagg gcgttgtggt gcagggcgag ctggaaaaag gcaatcatat
     8761 cctcgcgaaa gaagtgctgg cgaaacacga cgaaaactac acgccgccag aagttgagaa
     8821 agcgatggaa gctaaccacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
     8881 ccggaaattg gaaacgggct gctgtgtctg gcgttaggga ttgcgctgct gctgtccgtg
     8941 tatccgctat ggggcgtggc gcgcggagat gcgcgcatga tcgcgtcttc ccgcttgttt
     9001 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tgctggtcaa tgctttcgtg
     9061 gtcaacgact ttaccgtcac ctatgttgcc agctactcca atacccagct tccggtgtgg
     9121 tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tactgctgtg ggtgctgctg
     9181 atgagcggct ggacctttgc ggtggcgatt tttagtcagc gtattccgct ggatattgtg
     9241 gcccgcgtac tggcgataat ggggatggtc agcgtcggct ttttgctgtt cattctcttt
     9301 acctctaacc cattctcacg cacgctgccg aacttcccga ttgaagggcg cgatcttaac
     9361 ccgctgttac aggacccggg gctgatcttc catccgcctc tgctttatat ggggtacgtg
     9421 ggtttctcgg tggcgtttgc ttttgccatt gcttctttgc tgagcgggcg tctggacagc
     9481 acttatgcgc gttttactcg cccgtggacg ctggcggcgt ggattttcct gacgctcggc
     9541 attgtgctcg gttccgcatg ggcctattac gaactcggct gggggggctg gtggttctgg
     9601 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
     9661 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
     9721 gccttctcgt tgtgtctgtt ggggactttc ctcgtgcgtt ccggcgtgct ggtatcggta
     9781 cacgcgtttg cgtctgatcc ggcgcgcggt atgtttatcc tcgcctttat ggtgctggtg
     9841 attggaggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
     9901 aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct ggtcgccgcg
     9961 atgctggtgg tgttgctggg gacgctgctg ccgctggtgc acaagcaact gggactgggc
    10021 agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    10081 gcgctactgc tgggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    10141 cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtctctgct gttgccgtgg
    10201 ctgtttgaga gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    10261 gcggtgctgg caattgcgga agctgcgctg cgtatttcac gcggcacgaa aaccaccttc
    10321 agttattggg ggatggtggc tgctcacctg gggctggcag tgacaattgt tggcattgct
    10381 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat
    10441 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    10501 ggcggtgtgg cgactatcgg cgtaacgcga gagggtaaac cggaaacagt gctgtatgcg
    10561 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcgat tgacggtggc
    10621 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggctgtg
    10681 cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat gatggcgttg
    10741 ggcggactgc tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagtcc gcaaaaaact
    10801 gcgccggagg ctgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga
    10861 ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc
    10921 tggaatcggc gctcattggc aagccagtgc cgaagtttcg cctcgaatca ctggacaatc
    10981 cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtgctg cttaacgtct
    11041 gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttctgcgc
    11101 agggcatccg cgtagtcggc atgaactata aagacgatcg ccagaaggca atcagctggc
    11161 tgaaagagct gggcaatcct tacgcgctaa gcctgtttga tggcgatggc atgttagggc
    11221 tggatctcgg tgtctatggc gcgccagaaa cgttccttat tgacggcaac ggcatcattc
    11281 gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccgctgt
    11341 gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcatgc tgatgctgat
    11401 gatctccggc tcagcgctgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga
    11461 acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga acaacagcat
    11521 tgccgattcc aactcgatga ttgccaccga cctgcgccag aaagtgtatg aactgatgca
    11581 ggaaggtaaa agtaaaaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt
    11641 cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgctgc cagtagtagc
    11701 tattggcatt ggcggttggg tcatatacgc ccgctcgcgg cgtcgggtac gcgtggtgcc
    11761 ggaagcgttt cctgaacaaa gcgtgccgga aggtaagcgt gccggatatg tcgcttattt
    11821 gccgggcatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga ccggaaatta
    11881 tcaacaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc tggacagggc
    11941 gctggatccg aaagctgatc cgctcaacga agaagagatg tcgcgccttg cgctggggat
    12001 gcgtactcaa ctgcaaaaaa atccgggaga tatagacggc tggattatgt tgggccgcgt
    12061 tggcatggcg ctgggtaacg ccagtatcgc caccgatgca tacgctactg cgtatcgcct
    12121 cgatccgaaa aacagtgatg ccgcactggg ttatgctgaa gcgttgacac gttcatctga
    12181 tcccaacgac aaccgcctcg gcggcgaact gctacgtcag ctggtgagaa cggaccatag
    12241 caatatccgt gtgctaagca tgtatgcgtt taatgccttt gagcagcagc gatttggcga
    12301 agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata ctcgccgtgc
    12361 ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agagtaaata
    12421 aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg ttgttgcagg
    12481 aacagaatgg tggccgccac gcgtgagcgg acattgagtt tacgcagcag atttcgaata
    12541 tgaactttta ctgtctgctc ggaaatattc aacacagagg caatctgttt atttgacagc
    12601 ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct gaagggatct
    12661 tcttccgcgc caaacatttc acgttcacgt aagtactgat tgacgcgttc gctaaagact
    12721 ttgctgcctt tcgctccggc acgaatcgct tccagcaaca cttccgggtc gctgtctttc
    12781 agcagataac cgtctgcgcc tgcgtctatc agcgcaaaga catcgctgga ggcatcggat
    12841 acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc
    12901 aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc cagtctattc
    12961 gccagatcga tcgcgctcgc gccgtcgccc gcttcggcga ccacttcaaa gccaggatca
    13021 agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac aatcatcacc
    13081 tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat cattatgttt
    13141 gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta tgtatggaca
    13201 tgtagtgaaa actacaacct tgccgttcgc tttggcatca ttttcattat aaagacggaa
    13261 ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat acttgtgcat
    13321 ttctttaaac agtttttaaa aactgactcc gggtatggag ctatgggtat tttctgtacc
    13381 caatgctttt aacagcaatt aatttcatag gatgaaagct caatgcatca atctggttct
    13441 gtttctcttt gtcgttccgc aatatctgtt ctggtggcta cagcgttata ttcacccata
    13501 gcattggcat caacagttga gtatggtgag acagttgatg gtgttggcct ggaaaaagat
    13561 atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg agaacagcat
    13621 ataaaagaat ttggtgtaag tagtaatact gaaattaacg gcgggtatca gtacattgaa
    13681 atgaatggca ctgcagaata ctcagtatta aatgatggtt atcaaattgt tcaaatgggt
    13741 ggcgcggtaa accagactac gctcaataat ggagtgctac aggtttatgg cgctagactg
    13801 gccccctgaa tctccagaca accagtatca cttatttaag tgatagtctt aatactagtt
    13861 tttagactag tcattggaga gcagatgatt gatgtcttag g
//
LOCUS       DAZRVY010000106        13853 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZRVY010000106 DAZRVY010000000
VERSION     DAZRVY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.106"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHXOX_003151"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHXOX_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHXOX_003152"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHXOX_003152"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR7006551.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHXOX_003153"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHXOX_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR7006552.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHXOX_003154"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHXOX_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR7006553.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHXOX_003155"
     CDS             4177..5415
                     /locus_tag="ACHXOX_003155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR7006554.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHXOX_003156"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHXOX_003156"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR7006555.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHXOX_003157"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHXOX_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR7006556.1"
                     /translation="MSRPTIIINDLNAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHXOX_003158"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHXOX_003158"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR7006557.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHXOX_003159"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHXOX_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR7006558.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHXOX_003160"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHXOX_003160"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR7006559.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHXOX_003161"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHXOX_003161"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR7006560.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHXOX_003162"
                     /pseudo
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHXOX_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHXOX_003163"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHXOX_003163"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR7006561.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHXOX_003164"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHXOX_003164"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR7006562.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHXOX_003165"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHXOX_003165"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR7006563.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3506 c         3687 g         3259 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc attcaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tagtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZRVY010000107        13829 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZRVY010000107 DAZRVY010000000
VERSION     DAZRVY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.107"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1131
                     /locus_tag="ACHXOX_003166"
     CDS             <1..1131
                     /locus_tag="ACHXOX_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR7006564.1"
                     /translation="NIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQYDLD
                     KMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPELTT
                     PAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQVDV
                     SVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQNGVK
                     TKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGMTLA
                     EALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPY
                     DIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            1119..1565
                     /gene="etp"
                     /locus_tag="ACHXOX_003167"
                     /gene_synonym="yccY"
     CDS             1119..1565
                     /gene="etp"
                     /locus_tag="ACHXOX_003167"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR7006565.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            1585..3765
                     /gene="etk"
                     /locus_tag="ACHXOX_003168"
     CDS             1585..3765
                     /gene="etk"
                     /locus_tag="ACHXOX_003168"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR7006566.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(3880..5178)
                     /gene="appA"
                     /locus_tag="ACHXOX_003169"
     CDS             complement(3880..5178)
                     /gene="appA"
                     /locus_tag="ACHXOX_003169"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR7006567.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(5258..5350)
                     /gene="cbdX"
                     /locus_tag="ACHXOX_003170"
     CDS             complement(5258..5350)
                     /gene="cbdX"
                     /locus_tag="ACHXOX_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR7006568.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(5363..6499)
                     /gene="appB"
                     /locus_tag="ACHXOX_003171"
     CDS             complement(5363..6499)
                     /gene="appB"
                     /locus_tag="ACHXOX_003171"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR7006569.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(6511..8055)
                     /gene="appC"
                     /locus_tag="ACHXOX_003172"
     CDS             complement(6511..8055)
                     /gene="appC"
                     /locus_tag="ACHXOX_003172"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR7006570.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(8189..9046)
                     /locus_tag="ACHXOX_003173"
     CDS             complement(8189..9046)
                     /locus_tag="ACHXOX_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR7006571.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(9103..9441)
                     /gene="hyaE"
                     /locus_tag="ACHXOX_003174"
     CDS             complement(9103..9441)
                     /gene="hyaE"
                     /locus_tag="ACHXOX_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR7006572.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(9438..10025)
                     /gene="hyaD"
                     /locus_tag="ACHXOX_003175"
     CDS             complement(9438..10025)
                     /gene="hyaD"
                     /locus_tag="ACHXOX_003175"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR7006573.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(10022..10728)
                     /gene="hyaC"
                     /locus_tag="ACHXOX_003176"
                     /pseudo
     CDS             complement(10022..10728)
                     /gene="hyaC"
                     /locus_tag="ACHXOX_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(10747..12540)
                     /gene="hyaB"
                     /locus_tag="ACHXOX_003177"
     CDS             complement(10747..12540)
                     /gene="hyaB"
                     /locus_tag="ACHXOX_003177"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR7006574.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(12537..13655)
                     /gene="hyaA"
                     /locus_tag="ACHXOX_003178"
     CDS             complement(12537..13655)
                     /gene="hyaA"
                     /locus_tag="ACHXOX_003178"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR7006575.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         3355 a         3768 c         3629 g         3077 t
ORIGIN      
        1 aacattttta agttttcggt actgacattg gctgttctgt ctttgacagc atgtaccctt
       61 gttcctggtc agaatttatc gaccagtaac aaggatgtca ttgaactacc tgacaatcag
      121 tatgacctgg acaaaatggt caatatctat cccgtcacgc caggattgat tgatcagttg
      181 cgcgccaaac cgattatgtc gcaggctaac ccggagctgg agcagcaaat cgcaaactat
      241 gaatatcgta tcgggattgg tgatgtgttg atggttacgg tctgggatca cccggaacta
      301 accacccccg cagggcaata ccgtagcgcc agtgataccg gcaactgggt taacgccgat
      361 ggtgcgattt tctaccccta tattggtcgc ctgaaagtgg ccgggaaaac acttacccag
      421 gtacgcaacg agattacagc gcgtctcgat tcagtcattg aaagcccgca agtcgatgtc
      481 agcgtcgcgg cgttccgatc gcaaaaagcg tacgtcaccg gcgaagtcag caaatcgggt
      541 cagcagccga ttaccaatat tcctctgacc atcatggatg cgattaacgc cgcgggcggt
      601 ctgaccgcag atgcagactg gcgcaacgtg gtgctcaccc agaatggcgt aaagaccaaa
      661 gtgaacctct atgcgctgat gcagcgtggc gacttgcgcc agaacaaact gctgcacccg
      721 ggcgatattt tatttattcc gcgcaacgat gatctgaaag tcttcgtgat gggtgaagtg
      781 ggtaaacaga gcacgctgaa aatggaccgc agcggtatga ccctggcgga agcgctgggt
      841 aacgctgagg gtatgaatca ggatgtcgct gatgcgacgg ggatttttgt tattcgtgca
      901 acgcaaaaca agcagaatgg caaaatcgcc aatatttatc agctgaatgc gaaggacgca
      961 tcggcaatga tactcggaac ggaattccag cttgaacctt acgacattgt ttatgtcact
     1021 accgcgccgc tggctcgttg gaaccgtgtg atttctctgc tggtaccgac gatttccggg
     1081 gtgcatgacc tgacagagac ttcacggtgg attcaaacat ggcccaacta aaatttaact
     1141 caatcctggt ggtttgtacc ggcaacattt gccgttcgcc cattggcgag cgcttgctgc
     1201 gtaaacgtct gccgggcgta aaagtcaaat cggcaggtgt tcatggtctg ataaaacacc
     1261 ctgcagatgc gacagcggca gatgttgccg ctaatcatgg cgtatctctt gaaggacatg
     1321 ccggacgtaa gctcactgct gagatggcac gaaattacga tctgattctg gcaatggagt
     1381 cggaacatat tgctcaggtt accgcaattg ctcccgaggt ccgcgggaag acaatgctgt
     1441 ttgggcaatg gctggaacag aaagagatcc cggatcccta tcgtaaaagt caggacgcat
     1501 ttgaacatgt ctacggtatg ttggagcgcg ccagtcagga atgggcgaag cgcctcagcc
     1561 gataatttga gtttataaaa tacgatgaca actaaaaata tgaatacgcc accaggcagc
     1621 actcaggaaa atgagatcga tctgcttcgt ctggtcggcg agttatggga tcaccgtaag
     1681 tttattatca gcgtgaccgc gttattcacg ctgatcgctg tcgcttactc gctgttaagc
     1741 acaccaattt atcaggcaga tactctggtc caggttgagc aaaaacaggg caacgccatt
     1801 ctcagcggcc tgagcgatat gatccctaac tcatcgcccg agtctgcacc ggagatccaa
     1861 ctgctgcaat cgcgcatgat tctcggtaaa accattgctg aactgaatct gcgcgacata
     1921 gttgagcaga agtattttcc gattgtgggt cgcggctggg cgagattaac caaagaaaaa
     1981 ccaggtgagc tggcgatcag ctggatgcat attccacaac tgaatggtca ggatcagcaa
     2041 ctgacactca cggttgggga aaacggccac tatacactgg aaggtgaaga gttcaccgtc
     2101 aatggtatgg tcggccagcg tctggaaaaa gatggcgttg cgctgactat cgcggacatt
     2161 aaggccaaac caggaacaca gtttgtcctg agccagcgta ccgaactgga agcgattaac
     2221 gcattgcagg aaacctttac cgttagcgaa cgcagtaaag aaagcgggat gctggaactt
     2281 accatgactg gtgatgatcc ccagttgatt actcgtattc tgaacagcat cgctaacaac
     2341 tatttgcaac agaatatcgc tcgccaggcg gcgcaggatt cacaaagcct tgaattctta
     2401 cagcgccagt tacctgaagt gcgcagcgag ctggaccagg cggaagaaaa actcaacgtt
     2461 tatcgccagc agcgcgattc ggttgacctt aacctggaag ccaaagccgt tcttgagcag
     2521 attgtgaacg ttgataatca actcaatgag ctgaccttcc gcgaggcaga gatctcccag
     2581 ctgtataaga aagatcaccc aacttatcgt gcgctgctgg aaaaacgcca gacgctggag
     2641 caagaacgca aacgcctgaa taagcgggta tcggcaatgc cttccaccca acaggaagtg
     2701 ttgcgtttaa gtcgtgacgt agaagcgggc cgtgcggtat atctgcaatt acttaaccgc
     2761 cagcaggagt tgagtatttc gaaatccagt gccattggta acgtgcggat tatcgacccg
     2821 gcagtcactc agccgcagcc agtgaaaccg aaaaaagcgt tgaatgtggt gcttggtttt
     2881 attcttggcc tgtttatttc tgtgggtgcc gtgctggcgc gtgcgatgtt gcgtcgtggt
     2941 gtagaagccc cggaacaact ggaagagcac ggcatcagcg tttatgccac tatcccaatg
     3001 tccgagtggc tggataaacg cacccgtctg cgtaagaaaa atttattttc taatcagcag
     3061 cgccatcgta ctaaaaatat ccccttcctg gcggtggata acccggcgga ttctgctgtg
     3121 gaagccgtac gtgcgctacg aaccagtctg catttcgcta tgatggagac ggagaataac
     3181 attctgatga tcaccggtgc gacgccagac agtggtaaaa cgtttgtcag ttcaactctg
     3241 gcagcggtga tcgcccagtc cgatcaaaaa gtgttattta ttgatgccga cttacgccgt
     3301 ggttattcgc ataacctgtt taccgtgagt aatgaacatg gcttgtcgga atatctggca
     3361 ggtaaagatg agctcaacaa agtgatccag cattttggca aaggaggctt tgatgtgatt
     3421 actcgcggtc aggtgccacc taacccgtct gaactgctga tgcgcgatcg gatgcgtcaa
     3481 ttactggaat gggcgaacga ccattacgat ctggtgattg tcgatacgcc gccgatgctg
     3541 gcggtgagtg atgccgcggt cgtggggcgt tctgttggca ccagcctgct ggttgcgcgt
     3601 tttggcttga acaccgccaa agaggtgagt ttgtcaatgc agcgtctgga acaggcaggc
     3661 gtcaatatta aaggcgctat cctcaatggt gtgattaaac gcgccagcac cgcttacagt
     3721 tacggctata actattacgg ttatagttac tccgagaaag agtaaaactg aaagtggccg
     3781 gatgcggcgt gaacgccttt tccggtctac gcattagaca gttcttcgtt tgtcatcagc
     3841 ctcagagcat tcaggtaact gaatgctctt ttttatgcat tacaaactgc acgccggtat
     3901 gcgtgcttca ttcacgattt gcgtaaaacc tgccaacgaa cacatgccct gcgcatttcg
     3961 ctcttcacat cctgccaggg tcagtttcac ctctccgggc ggcgtattta atgacagcgg
     4021 cgttttatca cgcatctgct gtaaagtctg gaagaccagc gaaacctgaa tccactggct
     4081 gttatcgctt agccgacgcc agcgttcaaa caccagttca ccacctggcg gcgtgttatc
     4141 cggctgaccg ggaagcgtcc agttgagctc cagtgcgccg ccgagatttg ccagattagt
     4201 atcgtgtccg gcgataaaca gcactgaagt gggtaatgtc acaccatacg cctgtttttg
     4261 cggtggatgg ggcgtcaacg ctgtcttgat caaatctaat aacggggtgg cgcggctgcg
     4321 ggcaacctct ggcgtgcgtt gtagcaaata aaattgcgcg ttatgcaaac ttagcaaggt
     4381 gttccactgg tgtgaatcgg tgatccttcc ccaccccggc tccggcattc cctgtgcttg
     4441 ttgcaggaga aatatctccg tcagcattga tgcgaggctt accgcaccgg ttaatgagac
     4501 attgtcggcg ctcaccttga gttccgatgg taatgcctgc gttaatgaac agctttcgtc
     4561 ctgtttctca cgtttaaggc acaagtttga ttgcggaaaa ttaagcaccc gttccagttc
     4621 gcgaaacgcc gtttgccgat gcccggtaaa gtcagcaatt gaccctcctg ccctgctgag
     4681 gatcgcgtca gtcacgttcg cgttatccag ttggcaaacg ccagttttta gaggattaaa
     4741 taacggatcg ggactggacg tatcagcctg ggtatgtacg gttattgcac agtcaggtgc
     4801 cagcccggcg gcgaaggctt cgcctgtttt acgggtacgc tcgtcgacat cagcaataat
     4861 cgcgacctga ccagactgcg ggcagccctt tttcgccagc aatccgtcgg ctaccagacg
     4921 ctggcgttgg taatgtccga gataggcgat tagctcacca ccgcgcggtg tcagccaacc
     4981 cagttttacc ggccaggttg gccatgcgtc tggggtgaca tcctgcatca gttgcgtggc
     5041 cttggttgga gcacgcacac catgacgact gacaatcacc acactttcca gcttcagctc
     5101 cggctcactc tgagcgaatg cagattgcgg ggttaacgga atcagaagag ataaaaatgg
     5161 gattaagatc gctttcatcg atatgtttcc gcttttcata tctgacatta ttgattgcct
     5221 gatgcgatgc taatgcgccg catcaggcct acgttcgtta acttttcaga cgcgggtcca
     5281 gccataccaa cacaagggtg gagagcgaac acatcaacaa aatgccgacg aaccaaagta
     5341 aataccacat tgttcctgct ccttagtaca actcgttttc gttacggcgg agagtttctg
     5401 ttgtcatgcg tccccacatt ttgtagtagc tccagagagt gtagagcaac acaatgggca
     5461 aaaatatcag cacgattacc aacataatgc tcagcgtcag ctgactggaa gtgctgtccc
     5521 acaacgtcag gctggagatt ggactcacgc ttgacggcat gacaaagggg aacagcgtga
     5581 tgcctgccgt gaaaatcacg ccaaattgca tcaatgacgc cattaaaaat ccccaacccg
     5641 ggcgaccacg ataaatcgcc atcaccgtca gcaatgggca gaagaacccc agcagcggga
     5701 agatccacaa aacgggcgat tcgacgaaat tattcatcca ggcaccaggt agcactgcca
     5761 ccagtttcat taacggattg gaaggaccgt tagcatcctg ggcgagcagt acaaagccat
     5821 caataccgac ccacagccag taacccgcca gcaaaaagca cagcatcacc aacagtgcag
     5881 cgcgtttggt ggccagctgt gaacgcagat gaatcacacc aacagttttc agttgtaacc
     5941 agacgccacc ttgcaaaatc accatcccaa ggctgagcaa tccgcacaat aaagggaatg
     6001 gcgtcagcag ttgccagaag ctaccgagat actccacgcg taattgcggt gtgaaggcaa
     6061 acggcacgcc gagcaacaag ttgccgaagg cgataccgaa gactaccggc ggcaccagac
     6121 tgccgatgac cagaccggcg tcccacattt tacgccagcg tgcatcggcg atttttccgc
     6181 gataatcaaa ggccagcggg cggaagaaca gtgagcacag caccaggatc atcgccacat
     6241 aaaagccgga aaacgccgct gcatacactc tgggccaggc ggcaaataat gccccaccag
     6301 cgaggatcaa ccagacctgg ttgccttccc agtgtgcacc aacgctgttt atcactatcc
     6361 ggcgttcatc atcattacgc gccaccagcg gcagcagaca gccgatcccc atgtcaaatc
     6421 cgtcggagat cataaagacc accaggatca cgccaatcag cagccaccag atgaagcgca
     6481 atgtttcata atcaaacatg attttcccct ttacccctgt tgctgcgtcg gttgttcact
     6541 ctgcatcgcg ctcggcccca gacgggcata tttctgcatc aggtagactt cggcgattaa
     6601 gaacagggtg taaagcccta cgatcatgat cagtgagaaa gccagttgtc ctgtggttaa
     6661 agcggagtgc gcggagtatg tcggtaagat gtcctgtatc gcccacggct gacgaccaaa
     6721 ctcggtcata aaccacccgg cttcaatcgc aatccacggc aacggcaaac tccagagcgc
     6781 cattttcagc acccagcgat gctggtcgat tttgccacgc agcgtctgga caagcgcaat
     6841 cagcatcacc agtagcggca gggaaccaca gcccaccatg atgcggaaac tccagaatac
     6901 cggcgcaacc tgaggtatcg cgccacgcat cgccgcctgg tactgtgcgg ctgtgacatg
     6961 attcatatcc ggcgcatagc gggagagcaa catgccgtag cccaggtcac cttccagttc
     7021 ccggaatgcc tgcaacacat gcggctcacg attgccttgc gatatttcct gcattaacag
     7081 ccaggccata cgtccgcgtt gcaagcgtgg gtaggtttca gccatcaaat ttttcagacc
     7141 cggcacggtt ttatctaatg agtgagtggc gaggatccct aacagcgcgg gaattttgat
     7201 ggcaaaggcg ttacgctctt gatcctgttc cggccaggca accacatgga acggtgcagg
     7261 agcaggttcc gtttgccact ccccttccat cgccgccagt tttaccggtt gtacttgcgc
     7321 gacttcatac gcagaactgt ctccgagttg tagcgtgccg ataatcgcca gagtaccgaa
     7381 gacggaaccg atggcaaacg agcgtaatgc aacatcgcgc tcccgtccgc gcagtaaata
     7441 ccaggcgctg atcgccataa taaacatggc cccggtcacg tagcccgcca ttacggtgtg
     7501 cacaaatttc acctggctga ccgggttaaa gaccagctcg ctgaagctgg tcatctccat
     7561 acgtagggtg tcgatatcaa aatgcgcacc ggtcgggtat tgcatccaac cgttggcatt
     7621 caatatccac aatgcagaga gatttgaacc gaacgccacc agccacgtca ccagcaggtg
     7681 ctggtattta ttcagccgct gccagccgaa gaagaaaagc ccgacaaagg tggattcgag
     7741 gaagaaggcc attaatgctt ccatcgccag cggtgcgcca aaaatatcgc ccacatagtt
     7801 ggaatagaat gaccagttag taccaaactg aaactccatg gtcaggccgg tagccacgcc
     7861 aagagcaaag ttgataccga agagcttacc ccagaagcgc gtcatatcgc ggtagattgt
     7921 tttgccggtg accacgtaaa tggtttccat gatagccagc aaaaaaatca gccccagggt
     7981 aaggggtaca aataaaaagt gatacagcgc ggtcagagca aactgccagc gcgataaatc
     8041 aatgacatcc cacatgcgca ctcctgtagg cgaagagaga aaaataaccc gataatgtta
     8101 gatctccctc tttatcctga aactctacga ccagaggtat tcgccgattg cgatattcat
     8161 aagactgaaa gcgatactta ccgtcttttt acgtcggtgc agcttccgcc agccactgac
     8221 atacctcgct aagccgctgc gcagagtcga ccaaatcttc tggcgctgcc agcaccactt
     8281 ccggtattgg gcagatttca taactttcca gtaaaggacc ttttaaggtg tccgtacagc
     8341 gtaaatgcca gacatggcgt aaccccgtgg cgttgatata gctctcgcca tagccaatgg
     8401 tacgaatctg aatatttccc ggcccacaga gacgtgagag aaaaagccga tcagcctcgc
     8461 tgatgggtaa ttgcgtcaga ttaatgctgt gcggctgcgc gtcggggtta cgtacatgcg
     8521 ccagtaactc atgcgccaac ggtaggccat tcatcaggcc atcaatgggc ggcggtaaca
     8581 gcgaatctgt cggcaagaga ttttgcgtgg ccgcctgcca caacgccagc ggcgcgcagc
     8641 cggcctccag tttgtcctcc agcaactttt cgccgcgacg tttgcgcacc cgccataatc
     8701 cgcagaagat cgcctcctgt atttcacttt cgctgtcgtc agcctgctga atgcgcactg
     8761 acacttcccc ttccccgaga agcgtattga taaagtgacg atcgtcggcg ttgagattta
     8821 acagatcgac tgccagcacc tcgcccaacg gcggctggcg ttgacttagt tgatgttgta
     8881 actcgtttaa cagcgcaatc acctgcgggc tgtgagcaca ttgctccagg gccgccatac
     8941 tcggttcatc attcacctga cagctgatcg gcagtggatt catgctgaaa ctgtcatcgt
     9001 tcggttgcgt tcctggcccc agcagatgga aaaaagtttc gctcatgagg cacgctcctg
     9061 ttgtggttcg acaagcccac gcatcaggtt tatcagttcc gctcacgggt gaataccatt
     9121 cagcacaccg cgatagtttc cgccggtaaa cactaatgtg gcgggaaagc gaaagacgcc
     9181 aaagcgatct ccgatggctt cgctctgttc aaggtcagca atcgccacct gccatgtgta
     9241 gccgggaaac tcgcgcagta attcgccaat cattaccgga ttatcgctga cctctggcgt
     9301 gcgtttcggg tcactgctta ataacaccac gccgtctggc gcttgcgtaa gccagtcgtc
     9361 aagacggaat tcactaaccg gcgtccagcc gcgcgccagc attcgttgcc acaacgcatc
     9421 aaatggcgtg tcgttgctca tccctgctcc tccagtgtca tcctgtactg gcgcaagcga
     9481 acgccttcgt aattttccat cgacagacag tcataattga gacagcgcga ttcattagcc
     9541 ggttgcggca caattcccca tgcagccagc tgcgccagcg ccgcctgttc cgctgcgggc
     9601 agttgctccc gtgccagttc gctcaggcgg ccgccgtagt cgtcgagcat tgcgggttgc
     9661 agaccgacga gggcaatatg tgctggcaga tgtccgcgga tatctgccag cgccagcact
     9721 tcggagaaac tgttctggtg caggctcatt ttcttcgcgc tgagataagc cggaatacgt
     9781 tctccggcat aggttcgcag cgttccaggt tccagcccgt agtcaatggc atcgagaatc
     9841 aacagatggc tggcgctttc gacatacccc agcaagttca gtccctgagt accgccatcg
     9901 acaatctcca catactcggg ccagtggtaa tgggcataca accgttccgc cacccgcacg
     9961 ccgaaacctt catcggccca cagcaggttg cccagcccca ttaccacaac gcgttgctcg
    10021 ctcatgaacg ctccttgtta cttattttgc caaatttgtg gctacggtag ccgttgacca
    10081 tggtggagat caccgtgtcg tcggacatga tgtcttcacg cagcgccata tagacatgac
    10141 cgatcacaaa cgcgccaatc agccacatcc ccagccgatg ccagctgtga atgtccattg
    10201 agttgcctcc cgtccagtag aaaaattcca ccacataacg gaacggcgca aaaatagcgt
    10261 actggctgtg ttcgctgtac agcgcaaagc cggtgatgat cataaagacc gacatcagga
    10321 aatagccgaa catcgccgcc tgggcgatgg ggttatggcc tatatcggca ctcggacgtt
    10381 ttgccagaac agataccagc ggatttcata ccacacgccc tgccaccagc ttttacgcca
    10441 taccggcacg ataaacagct cgcgggagta tcgattgcca acaaaagccc agtagatccg
    10501 catcagcaag accacggtaa aaaccatccc ggcgctgaag tgaattaacc tgatgtagcc
    10561 catatagaac agatacgtcg cctcgccgct gacggaaggt agcggcttgc cgataaagta
    10621 tccggtgacc atcaacaccg ccatgcataa caccgtcagc cagtgccaga tgcggactgg
    10681 cgcttcaaag acatagtggc tgacaacgtt gtcgcttttc tgttgcatga tgattctcct
    10741 tcgctgttaa cgcacctgca cggagatcag ctcgctaccg tcgtcgccca gcacgtgtgt
    10801 tgaacaggcg aggcacgggt caaagctgtg cagagtacgc aggatctcca gcggttgctc
    10861 ggggatcgcc attttggtgt tcatcagcgc cgcttcataa gcgccaatct gccctttggg
    10921 atcgcgcggg ctggcgttcc aggtggtcgg caccacgcac tggtagagat caatcttgcc
    10981 atcgcgaatg gcggcccagt ggcctaacgc cccgcgcggc gcttcggtaa agccgacgcc
    11041 acggcactct gtcggccagg ttgcaggttc ccatttttcc gtggaggcgg tggcaagatt
    11101 gccgtttttc aggttagtca tcagcttgtt gaagaaatat tgcaacttac ctgcggccca
    11161 ctgtgcttcg tgcgcgcggc acaaaatgcg gcccaacgtt gactggatac cggaaagcgg
    11221 caggttcagc gccgacatca tgcgatcgac cgactcaacg gtcgcagcat cgcctttgtg
    11281 ataagcgatt aacgtgcgtg ccagcggccc cacttccatc gcgttaccgc gccagcgcgg
    11341 cgctttgatc cacgagtagc gttcctgttc attcagctgc tgaatgttgg tatcgctgcc
    11401 tttgacatcg ccggggttgt accacgggtc ggtaatgcca tcgaatggat gacgcccgac
    11461 ctgatcgttg ggataacgat accaggcgtg gtcgacaaac tcctgcacct gctgcggatc
    11521 aaccaaatcc actggcagca cattgttgaa gtcgccgtta atcaccgcgc cgccaggcat
    11581 cagcagactt ttctcaccaa agtcgttggc aatatccggg aacgcaccgt agctgaggac
    11641 acatttatca gaaagacccg tgccgatttc gctccacggt ttgttgaact gaccgatggc
    11701 taaggcgtcg gggatcatca cgttgttaat gaagtccgcc gtgcgggtga taatcgactg
    11761 caccaggttc aggcgttcca tattgaccgc cccgaccgcg ccgctttcgt caatgttaat
    11821 ggcgcaaggc atcccgccga caatccagtt tggatgcggg tttttaccgc caaagaccgc
    11881 gtggattttg acaatttcac gctggaaatc gagagcttcg agatagtggg caaagcccat
    11941 caggttagct tctggcggca gtttgtactg cgggtgcccc cagtagccat tgcggaagat
    12001 ccccaactgc ccgccttcaa caaatttttt caggcggttt tgtacgtcga agaaatagcc
    12061 aggggatgat ttcggccaag aggagagact ttgcgccagt tcggaggttt tccgcgggtc
    12121 ggctttcagc gcatctaaca catcgatcca gtccatcccg gcaagctgat agaagtgcac
    12181 cagatgatcg tggcaccaga gcgttgccag cataatgttg cggatgatat tggcgttgtc
    12241 cggcacttta ataccgatag catcttcgat ggcgtaaacc gaagccaggg cgtgtacgcc
    12301 agtacagacg ccgcagatac gttcaacgaa cgcccacgca tcgcgcgggt cgcgcccttg
    12361 taggatgatc tccagcccgc gaaacatggt gccgcaggag acggcattgg tgatcacatt
    12421 ctgatcgtta atattcactt cgcagcgcat gtggccttcg atgcgcgtaa tcgggtcgac
    12481 caccaggcgg cgtccggcat tattgatggt gtatccctga gtttcgtact gagtgctcat
    12541 gcctgtttat cctcattgcc tggctgatgt tcggtttctg taggttgctg gttatgacgt
    12601 ctgcgctggt caacggcgct ggcgactgcg tgcacaccaa cagccgctgc caccacacca
    12661 agcgcggtta aaccgacggt atcggcggtg gaatgagtac ccatttgcgg aatatcgacc
    12721 acgcggctgt agaacgaacc gcaatcccag aaaccatttt ccgcacagcc caggcagccg
    12781 tgaccagact ggattgggaa agaaacgcca tcattccagc gtgtggagga acaggcgtta
    12841 taggtggtag gccctttgca gcccattttg tacaggcagt aacctttgcg ggcagcgtca
    12901 tcatcccaac tctggacgaa ctctccggcg tcgaagtggg cgcggcgata gcatttatcg
    12961 tggattcgct gaccatagaa catcagcgga cggcccattc tgtcgacatc tggcaagcga
    13021 tcaaaggtca ccatgtaagt aatgatggcg ctcatcacat ccgggatcgg cgggcagcca
    13081 ggtactttga taatgggttt gtcggtgatg actttgtcga taggcgttgc ctgcgtcgga
    13141 ttgggccacg cggcctgcac gcagccccag gacgcgcagg ttccccaggc gataatcgcg
    13201 ctggctccgg cagcggcacg tttgagtttc tcaataaacg gtcgaccgct gctgatacag
    13261 aacatcccct gctcgcccag cggcggatta ccttctactg cgaggatata tttgccattg
    13321 tattgcgtga tgatgtcttc gaagacttct tccgcctggg ttccggcggc agccatcaaa
    13381 gtatcgtcat aatcgaggga aatcagggaa aggatgacgt ccttcgccag tgggtgagcg
    13441 gagcggataa aagattcggt acagcaggtg cattccagac cgtggatcca taccaccgga
    13501 atgcggggtt tgttctccag cgcccaggca atctttggtg ccattcccgc gcctaatccc
    13561 agcgacgtgg cagccagact acaatatttg agaaagctgc gccgggtaac gccctgacgc
    13621 cgcatggcct ggtaaaatgt ttcctcgtta ttcatatcgc acgtctcttc tccttgcgac
    13681 accggcagga caccgggcgc ttatggtcgg caagcgacgt ccctgtagca aggcaaacca
    13741 tcgcttcacg tgagatgctg aaaacgaaag ctcatccttc tgcactggcg cacgtcgcca
    13801 gaaagtattg ttaataaagc gtagtgaaa
//
LOCUS       DAZRVY010000108        13760 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZRVY010000108 DAZRVY010000000
VERSION     DAZRVY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.108"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHXOX_003179"
     CDS             complement(<1..80)
                     /locus_tag="ACHXOX_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006576.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGT"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="ACHXOX_003180"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="ACHXOX_003180"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR7006577.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHXOX_003181"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHXOX_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR7006578.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHXOX_003182"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHXOX_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR7006579.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHXOX_003183"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHXOX_003183"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR7006580.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHXOX_003184"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHXOX_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR7006581.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHXOX_003185"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHXOX_003185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR7006582.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHXOX_003186"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHXOX_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR7006583.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHXOX_003187"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHXOX_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR7006584.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="ACHXOX_003188"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="ACHXOX_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR7006585.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHXOX_003189"
     CDS             complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHXOX_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR7006586.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="ACHXOX_003190"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="ACHXOX_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR7006587.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="ACHXOX_003191"
     CDS             complement(11403..11900)
                     /locus_tag="ACHXOX_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR7006588.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="ACHXOX_003192"
     CDS             complement(11897..12259)
                     /locus_tag="ACHXOX_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR7006589.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="ACHXOX_003193"
     CDS             complement(12249..12596)
                     /locus_tag="ACHXOX_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR7006590.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /gene="yidI"
                     /locus_tag="ACHXOX_003194"
     CDS             12705..13154
                     /gene="yidI"
                     /locus_tag="ACHXOX_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR7006591.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13201..13359)
                     /locus_tag="ACHXOX_003195"
                     /pseudo
     CDS             complement(13201..13359)
                     /locus_tag="ACHXOX_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13403..>13760)
                     /locus_tag="ACHXOX_003196"
     CDS             complement(13403..>13760)
                     /locus_tag="ACHXOX_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7006592.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3266 a         3814 c         3571 g         3109 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgctaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ctccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc
//
LOCUS       DAZRVY010000109        13612 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZRVY010000109 DAZRVY010000000
VERSION     DAZRVY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.109"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            196..351
                     /gene="rseD"
                     /locus_tag="ACHXOX_003197"
     CDS             196..351
                     /gene="rseD"
                     /locus_tag="ACHXOX_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR7006593.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            348..923
                     /gene="rpoE"
                     /locus_tag="ACHXOX_003198"
     CDS             348..923
                     /gene="rpoE"
                     /locus_tag="ACHXOX_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR7006594.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            956..1606
                     /gene="rseA"
                     /locus_tag="ACHXOX_003199"
     CDS             956..1606
                     /gene="rseA"
                     /locus_tag="ACHXOX_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR7006595.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            1606..2562
                     /gene="rseB"
                     /locus_tag="ACHXOX_003200"
     CDS             1606..2562
                     /gene="rseB"
                     /locus_tag="ACHXOX_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR7006596.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            2559..3038
                     /gene="rseC"
                     /locus_tag="ACHXOX_003201"
     CDS             2559..3038
                     /gene="rseC"
                     /locus_tag="ACHXOX_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR7006597.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3236..5035
                     /gene="lepA"
                     /locus_tag="ACHXOX_003202"
     CDS             3236..5035
                     /gene="lepA"
                     /locus_tag="ACHXOX_003202"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR7006598.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5051..6025
                     /gene="lepB"
                     /locus_tag="ACHXOX_003203"
     CDS             5051..6025
                     /gene="lepB"
                     /locus_tag="ACHXOX_003203"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR7006599.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            6298..6978
                     /gene="rnc"
                     /locus_tag="ACHXOX_003204"
     CDS             6298..6978
                     /gene="rnc"
                     /locus_tag="ACHXOX_003204"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR7006600.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            6975..7880
                     /gene="era"
                     /locus_tag="ACHXOX_003205"
     CDS             6975..7880
                     /gene="era"
                     /locus_tag="ACHXOX_003205"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR7006601.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            7892..8620
                     /gene="recO"
                     /locus_tag="ACHXOX_003206"
     CDS             7892..8620
                     /gene="recO"
                     /locus_tag="ACHXOX_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR7006602.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            8632..9363
                     /gene="pdxJ"
                     /locus_tag="ACHXOX_003207"
     CDS             8632..9363
                     /gene="pdxJ"
                     /locus_tag="ACHXOX_003207"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR7006603.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            9363..9743
                     /gene="acpS"
                     /locus_tag="ACHXOX_003208"
     CDS             9363..9743
                     /gene="acpS"
                     /locus_tag="ACHXOX_003208"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR7006604.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            9965..10045
                     /gene="shoB"
                     /locus_tag="ACHXOX_003209"
     CDS             9965..10045
                     /gene="shoB"
                     /locus_tag="ACHXOX_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR7006605.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10239..10499)
                     /gene="yfhL"
                     /locus_tag="ACHXOX_003210"
     CDS             complement(10239..10499)
                     /gene="yfhL"
                     /locus_tag="ACHXOX_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR7006606.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            10714..11904
                     /locus_tag="ACHXOX_003211"
                     /pseudo
     CDS             10714..11904
                     /locus_tag="ACHXOX_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12089..13276)
                     /locus_tag="ACHXOX_003212"
     CDS             complement(12089..13276)
                     /locus_tag="ACHXOX_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR7006607.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(13276..>13612)
                     /locus_tag="ACHXOX_003213"
     CDS             complement(13276..>13612)
                     /locus_tag="ACHXOX_003213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006608.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3443 a         3398 c         3527 g         3244 t
ORIGIN      
        1 acacctgcta ttttaatatt tgttacagtg gctaaacacg ttgacgcagg gcggcgagaa
       61 aaagagaagt tactggctgg tggaggatta ggtggtgaaa taaaaaggcc gttgggttac
      121 tcttcaggca gttaaatggg catttctaca cagataatgc gatgttcaga ttctgtagac
      181 ttataatgat agataatgat ccgtctacag catgacaaac aaaaacagat gcgttacgga
      241 actttacaaa aacgagacac tctaaccctt tgcttgctca aattgcagct aatggagtgg
      301 cgtttcgata gcgcgtggaa atttggtttg gggagacttt acctcggatg agcgagcagt
      361 taacggacca ggtcctggtt gaacgggtcc agaagggaga tcagaaagcc tttaacttac
      421 tggtagtgcg ctatcagcat aaagtggcga gtctggtttc ccgctatgtg ccgtcgggtg
      481 atgttcccga tgtggtacaa gaagctttta ttaaagccta tcgtgcgctg gattcgttcc
      541 ggggagatag cgctttttat acatggctgt atcggattgc tgtaaataca gcgaaaaatt
      601 acctggttgc tcaggggcgt cgtccacctt ccagtgatgt ggatgccatt gaagctgaaa
      661 acttcgaaag tggcggcgcg ttgaaagaaa tttcgaaccc tgagaactta atgttgtcag
      721 aagaactgag acagatagtt ttccgaacta ttgagtccct cccggaagat ttgcgcatgg
      781 caataacctt gcgggagctg gatggcctga gctatgaaga gatagccgct atcatggatt
      841 gtccggtagg tacggtgcgt tcacgtatct tccgagcgag ggaagctatt gataacaaag
      901 ttcaaccgct tatcaggcgt tgacgatagc gggatactgg ataagggtat taggcatgca
      961 gaaagaacaa ctttccgctt taatggatgg cgaaacgctg gatagtgagc tgcttaacga
     1021 actggctcat aacccagaaa tgcagaaaac ctgggaaagc tatcacttaa tccgtgattc
     1081 aatgcggggt gatactcccg aggtgctcca tttcgatatc tcttcacgcg tgatggccgc
     1141 cattgaagaa gagccagtac gtcaaccggc gacattgatc ccggaagccc agcctgcgcc
     1201 gcatcaatgg cacaaaatgc cattctggca gaaagtacgt ccgtgggcgg cacagcttac
     1261 ccaaatgggc gtagcagcat gcgtatcgct tgcagttatc gttggcgtcc agcactataa
     1321 tggacaatct gaaacgtccc agcagcccga aacgccggta tttaatacac tgccgatgat
     1381 gggtaaagcc agcccggtaa gcctgggagt accttctgaa gcgaccgcaa acaatggtca
     1441 acagcagcag gtacaggagc agcgtcgtcg cattaatgca atgttgcagg attacgaact
     1501 gcaacgccga ctccactctg aacagcttca gtttgagcag gcgcaaaccc agcaagccgc
     1561 tgtacaggtg ccaggacttc aaactttagg aacgcaatcg cagtaatgaa gcaactttgg
     1621 tttgccatgt cattagtgac aggtagcctg ttattctctg ctaacgcctc ggccactccc
     1681 gcgtccgggg cgttattaca gcagatgaac ctggccagtc agtcactgaa ttacgagctg
     1741 tcattcatca gcatcaataa acagggtgtt gagtctctgc gttatcgaca tgcacgcctc
     1801 gataaccgtc ctcttgcaca attgttgcaa atggatggcc cgcgccggga agtggtacag
     1861 cgcggcaatg aaatcagcta ttttgaaccg ggacttgaac cgttcacgct taatggcgat
     1921 tacattgttg attctctgcc atcgcttatc tataccgatt tcaaacgcct ttctccttac
     1981 tacgacttta tctccgtcgg gcgcacgcgt attgctgatc gtctttgcga agtcattcgc
     2041 gtggttgccc gagatggtac acgctacagc tacatcgtgt ggatggacac tgaatcgaaa
     2101 ttaccgatga gggttgatct ccttgatcgc gatggtgaaa cgctggaaca atttcgcgtg
     2161 atttctttta acgtcaatca ggatatcagc agcagtatgc agacgctggc gaaggcaaat
     2221 ttgccgccgt tgctttctgt tcctgtaggt gaaaaagcta aattcagctg gacgccaacc
     2281 tggttgccac agggttttag cgaagtttcc agtagtcgac gtccgctacc gacgatggac
     2341 aacatgccta tcgaatcacg tctctattcc gacggattat tcagcttctc ggtaaacgtt
     2401 aaccgcgcta cgccatcgag caccgatcag atgttgcgca ccggacgcag aaccgtcagt
     2461 acaagcgtac gtgataacgc cgaaatcacc attgtcggtg aactgccgcc gcaaacggcg
     2521 aaacgcattg ccgagaatat taagttcggg gcagcgcaat gatcaaagag tgggctaccg
     2581 tcgtctcctg gcaaaacggg caggcgctgg tcagttgtga tgttaaagcc tcatgcagca
     2641 gctgcgcttc acgcgccggt tgcggtagcc gcgtgttgaa taaacttggc ccgcaaacca
     2701 cgcataccat tgtcgtaccc tgtgatgaac cgttagtgcc ggggcaaaaa gtggaattgg
     2761 ggatcgccga aggcagcctg cttagctccg cattactggt ttatatgtcg ccgctggtgg
     2821 gattattcct tatcgcttcg ctatttcagc tactctttgc ttctgacgtt gcagcattat
     2881 gtggtgcgat ccttggcggt attggcgggt ttctgattgc gcgcggttac tcgcgtaagt
     2941 ttgctgcccg ggcagaatgg caaccgatca tattaagcgt ggcattgccg cctgaccttg
     3001 tgcgatttga aacatcttcg gaagacgcga gccagtgatt tcactggctt tgttgcattt
     3061 tgcataacaa atgcgaggac gtttccagat accacaatcc atacggtatg aacatttcct
     3121 cgcctcaatg ttgtagtgta gaatgcggcg tttctattaa tacagacgtt aagctcagaa
     3181 cagcgacttc taaaagcctg gttaaccggg cattaaggca caataatcat actttatgaa
     3241 gaatatacgt aacttttcga tcatagctca cattgaccac ggtaaatcga cgctgtctga
     3301 ccgtattatc cagatctgcg gtggcctgtc tgaccgtgaa atggaggcgc aggttctcga
     3361 ttccatggat cttgagcgtg agcgtggcat taccatcaaa gcgcaaagcg tgacgctgga
     3421 ctacaaagcg tctgacggcg aaacctatca gcttaacttt atcgacaccc cgggccacgt
     3481 agacttctcc tatgaagttt cccgttcgct ggctgcctgt gaaggtgcat tgctggtggt
     3541 cgacgccggg cagggcgtgg aagcgcaaac cctggcaaac tgctacaccg ccatggaaat
     3601 ggatctcgaa gtggtgccag tactgaacaa gattgacctg ccggcagccg atcctgaacg
     3661 cgtggcggaa gaaattgaag atatcgtcgg catcgacgcc actgacgcgg tgcgctgttc
     3721 agcgaaaacc ggcgttggcg tgcaggacgt tctcgaacgt ctggtgcgcg acattccgcc
     3781 gccggaaggc gatccggaag gcccgttgca ggcactaatt atcgactcat ggttcgacaa
     3841 ctacctgggc gttgtttcac ttatccgtat taaaaacggc accctgcgta agggcgacaa
     3901 agtgaaagtc atgagtaccg ggcagaccta taacgccgac cgtctgggca tcttcacgcc
     3961 gaaacaggtt gaccgcactg aactgaaatg tggcgaagta ggctggctgg tatgtgcgat
     4021 taaagatatc cacggcgctc cagtcggcga taccttaacg ctggcgcgta atccggcaga
     4081 aaaggcgctg cctggcttta agaaagtcaa accgcaggta tacgccggtc tgttcccggt
     4141 aagttccgac gactatgaag ccttccgtga cgcgctgggt aaactcagcc tgaatgatgc
     4201 ctcactgttc tatgagccgg aaagctccag cgcgctgggc tttggtttcc gctgcggctt
     4261 ccttggcctg ctgcacatgg agatcatcca ggaacgtctg gaacgtgaat acgatctgga
     4321 tctgatcacc actgcgccga ccgtagtgta tgaagttgaa accacgtcaa gggaagttat
     4381 ctacgtcgac agcccatcca agctgcctgc ggtaaataac atttacgaac tgcgcgagcc
     4441 gattgcagag tgtcacatgc tgctgccgca ggcatatctc ggcaacgtta ttacgctgtg
     4501 cgtagaaaaa cgcggcgtgc agaccaatat ggtttaccac ggtaatcagg tggcgctgac
     4561 gtacgagatc ccgatggcgg aagtggtgct cgacttcttc gatcgcctga aatctacctc
     4621 gcgtggttat gcgtctctgg attacaactt caaacgcttc caggcgtccg acatggtacg
     4681 tgtagacgta ttaatcaacg gtgaacgtgt tgatgcgttg gcgctgatca cccaccgtga
     4741 taattcgcaa aaccgcggtc gcgagttggt ggagaagatg aaagatctga tcccacgcca
     4801 gcagtttgat atcgccattc aggcagcgat tggtacgcac attattgcgc gatccaccgt
     4861 gaaacagctg cgtaaaaacg tactggctaa atgttatggc ggcgatatca gccgtaagaa
     4921 aaagctgctg cagaagcaga aagaaggtaa gaaacgcatg aagcagatcg gtaacgtcga
     4981 gctgccgcag gaagcgttcc tcgccattct gcacgtcggc aaagacaaca aataaccctt
     5041 aggagttggc atggcgaata tgtttgccct gattctggtg attgccacac tggtgacggg
     5101 cattttatgg tgcgtggata aattcttttt cgcacctaaa cggcgggaac gtcaggcagc
     5161 ggcgcaggcg gctgccggtg actcactgga taaagcaacg ttgaaaaagg ttgcaccgaa
     5221 gcctggctgg ctggaaaccg gagcttctgt ttttccggtg ctggctatcg tattgattgt
     5281 acgttcgttt atttatgaac cgttccagat cccgtcaggt tcgatgatgc cgactctgtt
     5341 aatcggtgat tttattctgg tagagaagtt tgcttatggc attaaagatc ctatctacca
     5401 gaaaacgctg atcgaaaccg gtcatccgaa acgcggcgat atcgtggtct ttaaatatcc
     5461 ggaagatcca aagcttgatt acatcaagcg cgcggtgggt ttaccgggcg ataaagtcac
     5521 ttacgatccg gtctcaaaag agctgacgat tcaaccggga tgcagttccg gccaggcgtg
     5581 tgaaaacgcg ctgccggtca cctactcaaa cgtggaaccg agcgatttcg ttcagacctt
     5641 ctcacgccgt aatggtgggg aagcgaccag cggattcttt gaagtgccga aaaacgaaac
     5701 caaagaaaat ggaattcgtc tttccgagcg taaagagaca ctgggtgatg tgacgcaccg
     5761 aattctgaca gtgccgattg cgcaggacca ggtggggatg tattaccagc agccagggca
     5821 acaactggca acctggattg ttccgccggg acaatacttc atgatgggcg acaaccgcga
     5881 caacagcgcg gacagccgtt actggggctt tgtgcctgaa gcgaatctgg tcggtcgggc
     5941 aacggctatc tggatgagtt tcgataagca agaaggcgaa tggccgacag gtgtgcgctt
     6001 aagtcgcatt ggcggcatcc attaatagcc catcttcgtt cacgttgtcg ccgttatggc
     6061 gacaacgtga attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt
     6121 gtatagaata ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa
     6181 cagcgttggt ccccatatac cggtaaactg aaactgcagc gaagcagtta gcagagccat
     6241 gtatatcagg tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg
     6301 aaccccatcg taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa
     6361 ctgttgcagc aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa
     6421 tttttaggcg actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct
     6481 cgtgtggatg aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg
     6541 gcggaactgg cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt
     6601 aaaagcggtg gatttcgtcg tgagtcaatt ctcgccgata ccgtcgaagc attaattggt
     6661 ggcgtattcc tcgacagtga tattcaaacc gtcgagaaat taatcctcaa ctggtatcaa
     6721 actcgtttgg acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa
     6781 gaatatttgc agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc
     6841 gaagcgcacg atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg
     6901 gttggcacag gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa
     6961 aaactggagc tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac
     7021 gtccgaacgt tggcaaatcc acattgttga acaaactgct ggggcagaag atctccatca
     7081 cctcccgcaa agcgcaaaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt
     7141 atcaggcgat ctacgttgat acccctggcc tgcatatgga agaaaaacgc gctattaacc
     7201 gcctgatgaa caaagcggcg agcagctcta ttggcgacgt tgagctggtg atttttgtcg
     7261 ttgaaggcac ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgatggca
     7321 aagcgccggt gatcctcgcg gtgaacaaag tggataacgt gcaggaaaaa gccgatctgc
     7381 tgccgcactt acagttcctg gcaagtcaga tgaacttcct cgatatcgtg ccgatctctg
     7441 ctgaaaccgg gctgaatgtc gacactatcg cagcaatcgt gcgtaagcat ctgcctgaag
     7501 cgacccatca cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg
     7561 aaatcatccg cgaaaaactg atgcgtttcc tcggcgctga actgccgtac tccgtgaccg
     7621 tggagatcga acgtttcgtc tctaacgaac gcggtggtta tgacatcaat ggtttgattc
     7681 tcgttgagcg tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa
     7741 ccatcgggat tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg
     7801 aactgtgggt aaaagtgaaa tccggttggg ccgacgacga acgcgcactg cgcagtctcg
     7861 gttacgttga cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct
     7921 gcatagtcgc ccgtggagcg aaaccagcct gatgctggac gtcttcacgg aggaatcggg
     7981 tcgcgtgcgt ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt
     8041 acagcctttc acccctctct tgctacgttt cggcgggcgt ggcgaagtca aaacgctgcg
     8101 cagtgctgaa gctgtctcgc tggcgctgcc attaagcggt atcacgcttt acagcggtct
     8161 gtacatcaac gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt
     8221 tttcgattac ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc
     8281 gctgcgccgc tttgaactgg cacttctcgg gcatctgggc tatggcgtca attttaccca
     8341 ttgcgcgggt agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa
     8401 agggtttatc gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc
     8461 gttaaacgcg cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg
     8521 catggcgctt aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca
     8581 gtttatgcct aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa
     8641 ttactgttag gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct
     8701 tacccggatc cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc
     8761 gtgcatttac gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag
     8821 acgctggata cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc
     8881 gttgagacga agccacattt ttgctgcctg gtaccggaaa agcgtcagga agtaacaacc
     8941 gaaggcggcc tggacgttgc agggcagcgt gaaaaaatgc gcgatgcctg caaacgtctg
     9001 acagatgccg ggattcagat ttctctcttt atcgacgccg atgaagagca gatcaaagct
     9061 gcggcagagg ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa
     9121 actgacgccg aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct
     9181 agtctcggtc tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt
     9241 gccgccatcc ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg
     9301 atgaccggac tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc
     9361 taatggcaat attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga
     9421 tcgcccgatc cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct
     9481 ggaaaacgca ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg
     9541 cagcaaaagc gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat
     9601 tcaatgatga gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg
     9661 aaaagctcgg cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg
     9721 ccacggtaat tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa
     9781 ctggtaagga acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac
     9841 aacagcgatt atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa
     9901 aaactcaagc ctttacgaag aaagcaatac tgaacggctg cattccttgt ttaaggaaaa
     9961 acgaatgact gattgccgat acctgattaa acgggtcatc aaaatcatca ttgctgtttt
    10021 acagctgatc cttctgttct tataacacaa ggaaacgtac ttaaggtgcg tccggtgaac
    10081 caatcggacg cgcctttaat aactataaat aagtgtcttg gcagatacta tataaattaa
    10141 cttagtgaat gattatgcta atgtcatcaa ttaaataaat ataatggcgt taaggcttcc
    10201 cagtaatata attaatactc tacttccaga gtagaatatt aaattttatc cgcgtggtgc
    10261 atcagcacaa atttatccca caactgttct tctgtctcga catgcgccgg atctttcaca
    10321 atagtattgg ggatcgggca caccttctgg caggttggtg tctcgtagtg ccctacgcat
    10381 tcggtacact tatcgctgtt aatctcgtag atatgatctc ccattgaaat cgcctcattc
    10441 gggcattcgg gttcacacat atcacaatta atacagcgtt tagtaattag taaagacatt
    10501 tcaatggatt accgttaaat cattttaaaa tcagtaagtt gtatcgagtt tgtatgcttt
    10561 actgtcatta acttactgta tgttgatcca gtgtatttaa ccttgataaa ctcagtccag
    10621 caacacaaaa ccgcaacaca ttgcattttg tcccgtagaa aagacttgta tgtgtgagct
    10681 tgttttctgc gcctacgcag ataaggattg agaatgccgc gcactgtaac acataatccg
    10741 gatagcccca ataatgacga tgttttagcc gcatctgaaa aatgggacgc ctgtaaaccc
    10801 ccctatacca gcgcacacat gaaaatctgt gttgctgccg ccaaaatcat cctcgctgct
    10861 tccggcgtgg ctcgccgttc caaatacgaa aaagagaact atctccgtat cgatttcagc
    10921 aaagccggta aggttacatt ttacgccgag tttccaaaaa agatgggcct caaaggtaaa
    10981 aagctcggcg agtggccgga gctcgctatc cagctggcgc gcgaaaaagc actaggtatg
    11041 gctgacggtg gactgcgggc agagtccgta catgcagcgc tggaaatgta ccgggatgac
    11101 ctcaaagcca aagtagcccg gcagaagctg agcccggaca gtttcacaac ctacggggtg
    11161 cgtatcgacc ggattaaagc aacgttcggc gagcgtgagg tgttcagcga cgtaacatac
    11221 aatcggctgg tggaagtgct ggacgagtgg atcgccactc gctcgaacaa taacgccctg
    11281 gagttgtttg ccgagctccg tcggttctgg aagttctgcg cacctactct ttgcaacggc
    11341 cgcaatgttg ccgccagtct gccagatgat tatgtttcct cccgcgtaca gaaacctacc
    11401 cccacacggc tttttaccga tattgaatca atcgcccgac tctggctcaa tgttgctgcc
    11461 tgcacctctg tacaccagaa gaatgctgtt cgcttcatga tcatcactgg tgttcgtccg
    11521 attaatgtcc ataacctgcg ctgggactac gtttacgagg aggctggtga aattgtttat
    11581 ccggaagggg ttatcggcat gcgaggggct atgaaaacac aaaaggcttt ccgcctgccg
    11641 ataacgcctg agatccggcg gattatcgac gagcagaaag cctggcgtga ttcagttcct
    11701 gagtgcaaca gggattatgt atttttgcag ccacgtgatc caatgcagcc attttcaaaa
    11761 cgatcactgg ataagctggt gaaaacatac agcccggacg gggctgtaaa aggaataaaa
    11821 catgatggga ctgttaaagg gaaagacggt gcatttaata cgatgtgccg taaattcctt
    11881 aagagcaatg ttattgcctt gatcgatagg aattaaaacc ccaaaaagat taaaaaaaca
    11941 ccacaaaacg gatgtttctt caacaccact tttgctcata tgaacggaac cgacgattaa
    12001 actggatggc tctgattgat tcagggtatg aatggcggtt ttttgctccg tttccctcaa
    12061 aatggacgca actttccctc tgcggctctc agccgcacca ccgcatccgg gccagcggct
    12121 catgcatcag gacctgctct gccagacggt agccccgctt cagccccgta aaacgcatct
    12181 gactcccgca cagcacgcac ttcagcgggt caaccttcag taacctctga tacatccctc
    12241 tccaggtgat ttgcatcgcc gtttttctca ctgtctccgt tatgatgtac accacttctt
    12301 ccagtaaccg ccgtttcgcc ggactcaaaa aaccgtagta cctcaccata cggaacccct
    12361 tatccgccac atgccaggag aacctttcca tgaactcatc tccactcatc aacaggtatt
    12421 cttcccgttt tgttcggtga ctgttgtaac gcagaccgat ttcatcctga ccggcataat
    12481 gctccagacg actcatcggc accggtggct ttttcaggta agagccaaag tacaccgcca
    12541 catggggggc attatccatc acccgggata cgttgacatt ccagccacgg cggtaatgcg
    12601 tgtccaggaa gcgattccat tcccgtttac tgcttccttc tgctgccagc gcatccggca
    12661 tcaccaggtc agggtatttc cgtgacagca accgtgttat ccggtagcgc cacatgctca
    12721 tcaccttacg ggcgtaaaaa tgaagatttt tccaggtgtg gcccgacgtc acaccaccgg
    12781 cagttgtcga taaatggata tgcggatgcc actgctggtc acgcccccat gtgtggatca
    12841 ccgtgaatat ccccggctcc acatctgcct gatggcagat ttccagtatc acatccgctg
    12901 caatgcggct catctctgtc agtaaccacc ggttgtggaa caccagggac cagtactggc
    12961 agggaagtgt gaacacaata tgctgccacg ggcagtcggg gaccaggctc agcagatact
    13021 gtatccactg tgcgccagcc ttcaccccgc agtgcgggca ggagcggctt ttacaccgga
    13081 agcagacctt ttttgtatgg caacagtccg gtgatgaaca gcaccactgt gtatacccca
    13141 tcagtgtggt cccgcacgcc atgattttgg tcaccgactc aatcaccacc ggacgtactg
    13201 ccctttccgg ctgcttctcc agccagttaa gccagtggtt tccctgctga aagatatcgg
    13261 caaaacgggg aagcatcaga agggcggggc gactccgtcc ggccagtgaa ccgtgccaca
    13321 ctccgggcag tacataccgc cggcgctgat accggaaaga atggtcgcaa attcccgctc
    13381 cgtgcagcgg gcgatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag
    13441 ctttttgttt cccgcatcca caaagaacgg aatattcagg tctgcgcagc attcaacggc
    13501 atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct tcgtcaaaaa aatcatcttc
    13561 gtgaagcttc acgacatagc ggggaagttt gcttctttga gaggcgggtt ta
//
LOCUS       DAZRVY010000110        13570 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZRVY010000110 DAZRVY010000000
VERSION     DAZRVY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.110"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHXOX_003214"
     CDS             complement(<1..183)
                     /locus_tag="ACHXOX_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006609.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHXOX_003215"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHXOX_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1123..1224)
                     /locus_tag="ACHXOX_003216"
                     /pseudo
     CDS             complement(1123..1224)
                     /locus_tag="ACHXOX_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1408..1518)
                     /locus_tag="ACHXOX_003217"
                     /pseudo
     CDS             complement(1408..1518)
                     /locus_tag="ACHXOX_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
     gene            complement(1508..2479)
                     /gene="uxaB"
                     /locus_tag="ACHXOX_003218"
                     /pseudo
     CDS             complement(1508..2479)
                     /gene="uxaB"
                     /locus_tag="ACHXOX_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
     gene            complement(2706..3656)
                     /locus_tag="ACHXOX_003219"
     CDS             complement(2706..3656)
                     /locus_tag="ACHXOX_003219"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR7006610.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3795..4154)
                     /locus_tag="ACHXOX_003220"
     CDS             complement(3795..4154)
                     /locus_tag="ACHXOX_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR7006611.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(4154..5080)
                     /gene="glsB"
                     /locus_tag="ACHXOX_003221"
     CDS             complement(4154..5080)
                     /gene="glsB"
                     /locus_tag="ACHXOX_003221"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR7006612.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5144..6532)
                     /gene="sad"
                     /locus_tag="ACHXOX_003222"
     CDS             complement(5144..6532)
                     /gene="sad"
                     /locus_tag="ACHXOX_003222"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR7006613.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            6633..7514
                     /locus_tag="ACHXOX_003223"
     CDS             6633..7514
                     /locus_tag="ACHXOX_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7006614.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            7591..8706
                     /gene="yneK"
                     /locus_tag="ACHXOX_003224"
     CDS             7591..8706
                     /gene="yneK"
                     /locus_tag="ACHXOX_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR7006615.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            8856..10046
                     /gene="ydeA"
                     /locus_tag="ACHXOX_003225"
     CDS             8856..10046
                     /gene="ydeA"
                     /locus_tag="ACHXOX_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR7006616.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(10071..10736)
                     /gene="marC"
                     /locus_tag="ACHXOX_003226"
     CDS             complement(10071..10736)
                     /gene="marC"
                     /locus_tag="ACHXOX_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR7006617.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            10948..11382
                     /gene="marR"
                     /locus_tag="ACHXOX_003227"
     CDS             10948..11382
                     /gene="marR"
                     /locus_tag="ACHXOX_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR7006618.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            11402..11785
                     /gene="marA"
                     /locus_tag="ACHXOX_003228"
     CDS             11402..11785
                     /gene="marA"
                     /locus_tag="ACHXOX_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR7006619.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            11817..12035
                     /gene="marB"
                     /locus_tag="ACHXOX_003229"
     CDS             11817..12035
                     /gene="marB"
                     /locus_tag="ACHXOX_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR7006620.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(12092..13531)
                     /locus_tag="ACHXOX_003230"
     CDS             complement(12092..13531)
                     /locus_tag="ACHXOX_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR7006621.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3588 a         3419 c         3047 g         3516 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctcttttaa
      901 taggaaaacc ttgctacaaa gtaaatattt taaataacag taagtttaac tactgttgtt
      961 tattgatagt ttagatatta tgtgattttt catagcgctc caaatacaaa ccactcctca
     1021 ttaataatga gattcacatt ggtgaattgc tcgggtgtgc gtagccatgc cacacacacc
     1081 ctgtttcaat ttcagatgac gtatagccac cttaagattg aattatagat attgaacttt
     1141 gaaggtcgca tcagcgtttg cagtaccagc tacggactgc ccgagagcaa tataacgagc
     1201 ctggaatggc agtatattcg tgccacgata acatctttaa atcacaagga cacctaatct
     1261 gaacgaataa cgactaccag aaaagcactt cgcatcactc agacatgatt taacataata
     1321 tacattatgc gcacaaacat aaaccaaggg agaatccagt cgttggccgg tcatgattgc
     1381 tcattaacaa cgaccaaacc ccatatctca cttactaccg tattctcggc ttaaccgtcc
     1441 atgcaacctc aacacattgg tttcaactgc cgtcaccacg ttctccggaa aatctgtcgg
     1501 taatgaagtc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
     1561 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
     1621 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
     1681 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
     1741 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
     1801 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
     1861 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1921 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc gatcaactca cacgggatga
     1981 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     2041 tcagttttgc cggatagctt atcgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     2101 tgatccccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     2161 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     2221 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtatacagac
     2281 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     2341 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     2401 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     2461 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     2521 ggtacgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     2581 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     2641 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     2701 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     2761 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     2821 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     2881 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2941 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     3001 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     3061 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     3121 atgaccgtca gaggcacgat caacacgccc gttgagaatt gctcactgaa ccagtccgca
     3181 agcaaaggcc agaaatccag actatcaata ctgaccgaac caatcgcccc gacaatagcg
     3241 cataataatg cggcaatcag acagtaatta aatagccgta aggcacttac tggctcatac
     3301 ttatttttcc ccagacgctt atcccgcacg accagtaagg cgacggtaac aataaacatc
     3361 atattggaga aattgataac cagtgaaacc agtccccatt cggtggttat ggcatcataa
     3421 accagcatcg ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg
     3481 gcaaaccccc ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagc
     3541 cgtaactctg caccaataaa atagaacagc gtggttagcg caaaaatagc gatgctatta
     3601 cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa tggggtgtac atgcattaat
     3661 tatctgtcca tacaattgcg ggtggcaatt atctcaacgt aataagtaaa ttctcataaa
     3721 ggatagcatg ttcgctaaca gcggcggcaa ataaaggtac cctgggaatg ctattttatc
     3781 gttttccgca attctcatgg ctgattcatc tgtacaacca gccagtgata gattaccgcc
     3841 acgatataac gttgttgttc ttcgcttaac gaaacaccct gcggaatgtt atgccactta
     3901 gccagacaac cgcgacagca ggtcgcggtg gcgtgctgag cgataaacac cggatgcccg
     3961 cgcatggggg tttgcttacc gtcattagcc ggtaatgccg gagcaagacg tttagcgaca
     4021 aaatccgccg catgttgttc gatgactggc gcgcctttct ccaggcaata ctgacgctct
     4081 ttcatgccca gacgaaagcg agagcgaaat tttgaacggg ataaacgcgc gaagagtgga
     4141 tcgagagact gcattaataa accgaacgcc ctaactgttt cgtcaattgt tcaaggacgg
     4201 cgatacccgc aagcgagtta cctgcatcat ccagttccgg actccagact gcgatggcca
     4261 tttcatgcgg aacaatcgcc acaataccgc caccaacgcc agatttcgcc ggtagtccca
     4321 cccgccaggc aaactccccc gcgttctggt acataccact ggtcgccatc agcgcgttaa
     4381 tttgccgcgc ctgcattggc gtcactacag gttcatcaat atgaatagct ttcccctgat
     4441 tagccagaaa gacaaacgtc cgagccagct ctacacagct cattttcaga gcgcagtaat
     4501 gaaagtagtt ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca
     4561 gccaggcgat agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat
     4621 cgtatgaaat atcagacaca ccgcttaagc cgcgcacgac ttccagcata cgttgccgtg
     4681 gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag cgcaccagca ttaataaacg
     4741 gattacgcgg aataccctgt tccatttcca gttgcactaa agaattgaac ggtgatccag
     4801 acggatcttt gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa
     4861 cgagactcag cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct
     4921 gaaaaagctg tccgtcaacg gtacagatag caatgcccaa tcgggaaccg tctactgtag
     4981 ccagcgccgg aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca
     5041 agatgttttc taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag
     5101 gtctgaaaag acctgcgagt atatcagagc tgaatatgtc gcgtcagatc cggtctttcc
     5161 acaccgtctg gatattacag aattcgtgta agccgaaatg ggaaagctca cgaccaaagc
     5221 cactcttttt cacgccacca aaggccactc gcgcgtcgct ggcacaataa ccattgataa
     5281 acaccccacc gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtaa
     5341 aaatggtcgc tgaaaggccg aactcactat cattagccag ttccagcgca tgttctgcat
     5401 ctttcgcaac ggtgattgcc gcaacggggc caaacatttc ttcccgaaac gcggtcattt
     5461 ctggggtaac attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt
     5521 ccccgcccag taacaaacgc gcaccctgcg ccagggtttt ctctacctga tgatgcagct
     5581 catcacgtaa atcaaaacga gccattggtc cgagagtgtt ctcttcgtca cggggatcgc
     5641 ccattttcaa ggctgccgca gctgccacaa aacgttcggt aaatgcagaa gcaattccct
     5701 cttcgataat aaagcgtttt gccgctgcac atacctgtcc ggtattctga taacgtccgg
     5761 ctaccgccgc tttaaccgcc agttccagat cggcatcgtt aagcacaata aacggatccg
     5821 aaccgcccag ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg
     5881 ctccagcacg aacacttccg gtcaccgtga cagcagcaat gcgcgagtct ttaatcatct
     5941 gactgacacc gtcgttgtcg gcattcagcc agccatatac gccttgcggg attcccgcat
     6001 ctttaaacac ctgggcaatg agctgagcac atcccatcac attcggcgca tgtttaagta
     6061 agtagccgtt acctgcaaga atgatgggaa cagcgccacg catcacctgc cataacggaa
     6121 aattccacgg cataatcgcc agaatcgtcc ccaacggtcg atactcaata accgcctgct
     6181 gattttccac cagtgtaggt tccgccttca gcattgccgg accatgttct gcataccagt
     6241 cacacaaatt cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt
     6301 cgcgggtgat catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgatatcac
     6361 gcattttttc agcacgataa tctatatttg tctcgcgcca gtcgcgaaag cctgctgccg
     6421 ccagctcaag tgcgttttcg atatcgttag cgccagccca cggcagcaca gaaagttgtt
     6481 cacccgtggc aggatttatt gaaattgcat gagttgccgg agtaatggtc atcggggtat
     6541 ctcctttatg agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat
     6601 attaagccac ttattcacga atcgagaatg ttatggatct gacccaactg gagatgttca
     6661 acgcggttgc cgaagctggc agcattaccc aggctgcagc aaaagtgcat cgcgtaccgt
     6721 cgaatctcac tacccgtcta cgccagctgg aaacagaact gggggtagat ctgtttattc
     6781 gcgagaatca gcgtttacgt ctctctcctg ccgggcataa ctttttacgc tatagccaac
     6841 aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt
     6901 tattttctct tggttcgctg gaaagcaccg cagcagtgcg cattccggcc acgctggcgg
     6961 aattcaacca tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta
     7021 tgctggaagg agtgctggag ggaaaactga atgcggcatt tattgatggg cccattaacc
     7081 atactgccat tgacgggata ccggtatacc gcgaggagct gatgatcgtc acgccacaag
     7141 gtcatgcgcc agtaatccgc gccagtcagg ttaacggcag taacatttat gccttccgca
     7201 ccaattgttc gtatcgtcgc cacttcgaga gctggtttca tgctgacggt gccgctccgg
     7261 gaactattca cgagatggag tcgtatcacg ggatgttggc ctgtgtggtc gcaggagcag
     7321 gcattgcgct tattccacgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag
     7381 cgtggccgtt agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgcggtg
     7441 cgaaaacgcg tccgcttgag gcatttattc aactactgga tgtgtctgac tcggcaaaac
     7501 agagttacca atgaactatt ttgatagttc ttgcggctaa tatgctctat atagtgatgt
     7561 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
     7621 atatctcttc ctgatgattt tcccgttcgt aatattgcac cacaggtaaa agaagtttta
     7681 aaggacttta ttgatgcgct aagtacaata atatgtgatg aagagtggcg cacgtcttta
     7741 aacatcaact cagccacaaa aaagatattt aataaccttg ataacttatc atatattcag
     7801 aggacttctt tccggggtaa cgacacgcta tacaatgaaa aggttcagtt taaactcact
     7861 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
     7921 agccctattt atttagataa ttttcgccat gatggagaaa ttaatatttt ttgcgctccc
     7981 aacccaaagc ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg tgtgctcttt
     8041 ctttttctga atgattttat tgaacaattt ccaatgttca accctggcgt tccgatcaaa
     8101 atagcgcata caccacatat tgaacccctg cctccggatc accataccgc tgcagattac
     8161 ttacgccagt ttgatttgct tgtcctgaat tttatctctc gcggtaattt tgtcatactc
     8221 ccccgattat ggaataactc tgaggttcac agatggttcg tcaataaaga tcctaacttg
     8281 atcaccgcca ttctcgacat aacggacagc gaattaaaag aggatttatt gcaaagcctg
     8341 atggattcat taggttctaa caaacatgta ctacccgaag tctgcatctg ctttttattc
     8401 cttttagcag aacaagaatc tcctcatttt caggacttgt ttttattttt cgccaatatg
     8461 ttattgcact atcaccaatt tatgaatccc aatgaaagtg atttgaatga cgtgttgatg
     8521 ccagcatcat taagtgatga taaaattatc aagcatatgg cgcgcaggac cctcgaactg
     8581 tttgtaaaaa atgaaacacc gccaaaagtt actcacgaag acctggtgaa aaacaggcct
     8641 cgctcccctg tcagaccacc tatacccgca acctccaaaa cgccagacct ccctgaacgt
     8701 cattaaaccg tgatgttacc gactctctga cgcggaacag aatcagcgtc agagaaacgg
     8761 aaaacgcgat ccagatcaca aatacattgt attcacatca ttaaccgttt taagatcatt
     8821 tcatcacttt ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca
     8881 aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg
     8941 aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc
     9001 aggtcggcat catgttgacc atttacgcat gggtagtagc gctaatgtca ttgcctttta
     9061 tgttaatgac cagtcaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta
     9121 ttgccagcca cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca
     9181 ttggtgtggc ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta
     9241 tggctccggc cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg
     9301 cgatggtctt aggtttacct ctcgggcgca ttgtggggca gtatttcggt tggcgaatga
     9361 ccttcttcgc gattggtatt ggggcgctta tcaccctttt gtgcctgatt aagttacttc
     9421 ccttactgcc cagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc
     9481 cggcattgat gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat
     9541 acagctatat cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg
     9601 cattactgtt attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg
     9661 gtaatcagta tgcgtctgcg ttggtgagta cggcgattgc gctgttgctg gtgtgtctgg
     9721 cactgctgct acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg
     9781 ggatcgcgat gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag
     9841 atgctaccga cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg
     9901 gtgcgttggt aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg
     9961 gcgcggtgcc tgcttttgcc gcgttaatat ggtcaatcat tatatttcgc cgctggccag
    10021 tgacactcga agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac
    10081 gttttaatga tttccaggat gccgttaata ataaactgca cacccataca taccagcagg
    10141 aatcccatca gacgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg
    10201 ccggagctgc gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc
    10261 gcaaccatca gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta
    10321 ataatcatcg ctatggttcc cggaccagcg gtacttggca ttgctagcgg cacaaaggca
    10381 atattggcac tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca
    10441 atcgctttct gttgcggaaa gagcatccga aaaccgataa acgcgacgat taagccgcct
    10501 gcaattcgca gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa
    10561 tacgccacca tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt
    10621 tcggcactgt tcatgttgcc tgccaggcca agaaatagcg cgacagttgt taatgggtta
    10681 gctaacggca gcaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt
    10741 ggttgttatc ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa
    10801 gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttagcaaaa
    10861 cgtggcatcg gtcaattcat tcatttgact tatacttgcc tgggcaatat tatcctctgc
    10921 aactaattac ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt
    10981 attccattgg gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag
    11041 tatctgtctc cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc
    11101 gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactg
    11161 acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat
    11221 gataagcgcg gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc
    11281 catcaattag ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa
    11341 gttgcaacac ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac
    11401 gatgtccaga cgcaatactg acgctattac cattcatagc attttggact ggatcgagga
    11461 caacctggaa tcgccactgt cactggagaa agtgtcagag cgttcgggtt actccaaatg
    11521 gcacctgcaa cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag
    11581 ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct
    11641 ggcagaacga tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt
    11701 tgatgttccg ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca
    11761 tccattaaat cattacaata gctagttgat aacgtgacaa cgtcactgag gcaatcatga
    11821 aaccactttc atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg
    11881 aacaaaccac gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc
    11941 aggaacaacc accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc
    12001 tcggcgtgcc ctattataat caacacgcta tgtagttatt gtcaggctct gatcacagag
    12061 cctgacatct ccgtaatatc ttcctcaagt ctcaaatatt ttcgccattg ttggcgatga
    12121 gatcccgata ccagtaaaac gacgctttac gtatccgttc cagcgttccc ttcccttcgt
    12181 tgtctttatc gacataaatc attccgtagc gctttttcat ttcccctgtt ccggcagaaa
    12241 ccaggtcaat gcagccccac ggggtgtagc caattaagtc aacaccatct tcaactacgg
    12301 cttttttcat ttcccgaata tgggaagcaa agtaatcaat gcgatagtga tcgttcaccg
    12361 tgccgtcagc ttgtctctgg tcaaccgcac caaatccatt ttcgacaata aacagcggca
    12421 actggaaatg atcccagaac cagttgagtg aataacgtag ccctgccgga tcaatttgcc
    12481 atccccattc ggatgtatcg atataagggt tgctgaccaa atctcgtggt tcaacataat
    12541 ccagttgcgg gttatcggga gaaaattgtg tcgtaaaaga catgtagtag ctaaaaccga
    12601 taaagtcgac acatccactg gcaagaatcg cgttatcttc tggtgtgata tcgaggttga
    12661 atcctttcct ggcaaagtaa ttcagcatat gttgcggata atatccacga gcatgaacat
    12721 cagtaaacca gtaacgacga tgcatcgctt tcgtggccat catcatatcg ttgggtgcac
    12781 acgtcagagg atagatgggg cacatggcga tcatacagcc aatgttaaat tcaggattga
    12841 tcgattttcc agtctgtacc gccagggcac tggcaactaa ctcgtaatgt accgcctggt
    12901 acattatttg ttcgcgctca ttgatatctt cctctggcga atacaagata ccggaattag
    12961 taaatggaca caggctttcg ctgaaattca cctgattatt gatttcgtta aacgtcatcc
    13021 agtactttac tttttcttta tagcgcgtga agaccgttga tgcgaagcgg ataaaaaagt
    13081 cgatcagctt acggtttcgc cagccaccat attttgtcac cagatgataa ggcatctcaa
    13141 aatgcgaaag cgtcaccaca ggttccattc cttgcttcag gcattcatcg aacagatcat
    13201 cataaaattg taaaccctct tcattcggct cctgttcgtc accttgcgga aagattcgtg
    13261 tccaggcaat ggaagttcga aagcatttga atcccatctc ggcaaataac tgaatatctg
    13321 ttttatagcg atgataaaaa tcaattgctt catgattggg ataattaagc ccgtcgataa
    13381 cgccttctgt cacttcacgc ggcaccccgt gagcgccagc agtcatcaca tcagcgatac
    13441 tgatgccttt tcctccttca ttccagccac cttccaactg atgcgcggct accgcgccac
    13501 cccataaaaa acctttttta aatcctgaca tacacaactc cttaactaaa atagattacc
    13561 accagacttc
//
LOCUS       DAZRVY010000111        13549 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZRVY010000111 DAZRVY010000000
VERSION     DAZRVY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.111"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHXOX_003231"
     CDS             complement(<1..312)
                     /locus_tag="ACHXOX_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR7006622.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHXOX_003232"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHXOX_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR7006623.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHXOX_003233"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHXOX_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR7006624.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHXOX_003234"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHXOX_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR7006625.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHXOX_003235"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHXOX_003235"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR7006626.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHXOX_003236"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHXOX_003236"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR7006627.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHXOX_003237"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHXOX_003237"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR7006628.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHXOX_003238"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHXOX_003238"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR7006629.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXOX_003239"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXOX_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR7006630.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXOX_003240"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXOX_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR7006631.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXOX_003241"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXOX_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR7006632.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXOX_003242"
     CDS             complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXOX_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR7006633.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3599 a         3420 c         3035 g         3495 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
    13381 aaaataatat taattacgca aaaataaaat ataaacttat tgtttaaata ttatttaaac
    13441 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
    13501 agatagatga atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAZRVY010000112        13174 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZRVY010000112 DAZRVY010000000
VERSION     DAZRVY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.112"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..296
                     /locus_tag="ACHXOX_003243"
     CDS             <1..296
                     /locus_tag="ACHXOX_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR7006634.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            791..1690
                     /gene="lysO"
                     /locus_tag="ACHXOX_003244"
     CDS             791..1690
                     /gene="lysO"
                     /locus_tag="ACHXOX_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR7006635.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1834..3486
                     /gene="hcp"
                     /locus_tag="ACHXOX_003245"
                     /gene_synonym="priS"
     CDS             1834..3486
                     /gene="hcp"
                     /locus_tag="ACHXOX_003245"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR7006636.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3498..4466
                     /gene="hcr"
                     /locus_tag="ACHXOX_003246"
     CDS             3498..4466
                     /gene="hcr"
                     /locus_tag="ACHXOX_003246"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR7006637.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4599..6317
                     /gene="poxB"
                     /locus_tag="ACHXOX_003247"
     CDS             4599..6317
                     /gene="poxB"
                     /locus_tag="ACHXOX_003247"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR7006638.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6354..7355
                     /gene="ltaE"
                     /locus_tag="ACHXOX_003248"
     CDS             6354..7355
                     /gene="ltaE"
                     /locus_tag="ACHXOX_003248"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR7006639.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7366..8796
                     /locus_tag="ACHXOX_003249"
     CDS             7366..8796
                     /locus_tag="ACHXOX_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR7006640.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8895..9908
                     /locus_tag="ACHXOX_003250"
     CDS             8895..9908
                     /locus_tag="ACHXOX_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR7006641.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="ACHXOX_003251"
     CDS             complement(9905..10735)
                     /gene="amiD"
                     /locus_tag="ACHXOX_003251"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR7006642.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10732..11055)
                     /locus_tag="ACHXOX_003252"
     CDS             complement(10732..11055)
                     /locus_tag="ACHXOX_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR7006643.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11266..13035)
                     /locus_tag="ACHXOX_003253"
     CDS             complement(11266..13035)
                     /locus_tag="ACHXOX_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7006644.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKFINCHFFNVDMIRVNFSKCLFH
                     DCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRY
                     IPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         3155 a         3365 c         3287 g         3367 t
ORIGIN      
        1 cgctggtagt tgaactggta ttgagtgcag gtttcctgtt ggtgatccac ggcgcaaccg
       61 acaaattcgc gccggcaggt tttgcgccga tcgctattgg tctggcctta accctgattc
      121 acttaattag tattccggtg actaacactt ctgttaaccc ggcgcgcagc accgcggttg
      181 ctatcttcca gggcggctgg gcattagaac aactgtggtt cttctgggtg gtgccaattg
      241 tcggcggcat tatcggtggt ctgatttacc ggaccctgct ggaaaagcgt gattaattaa
      301 cgcagaaggc ctggcgtaat gtcaggcctt tttttaagat gttgttatat gttgattcat
      361 catcttgttg cgctcctttc atgagctaag ccacatattg ccactggcgc aaggagcgcg
      421 cagggggcgg ccaatcgccg ccgccccctg cccccccggg ctctggcgaa caaaatcgcc
      481 gctgcgcggt gccctcgact tatcccttac ggctaccggg tcgggcgcga ggtaacatcc
      541 ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttca gggaacgcct
      601 cggcgatttt gacgccacca acatcggtgc agtttgtaat taaagcgcaa gaatatcttt
      661 aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa ttgcaacttt
      721 actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc tttgcaagga
      781 ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg taggttacct
      841 cattccgctt cgccaacaag ctgcgttaaa agttattaat cagctattaa gctggatggt
      901 ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc tcgccagtaa
      961 cctgttggcg attctgcatt attctgccgt cagtattacc gttattttac tgtgtaatat
     1021 tgccgccctg atgtggctgg agcgaggcct gccgtggcgc aaccaccatc agcaagaaaa
     1081 actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg tagtagtgat
     1141 tggttttgcc attggtctga gtggactggc tttcttacaa cacgcgaccg aagccagtga
     1201 atacacgtta attttgctac ttttcctcgt tggtattcag ttgcgcaata atggcatgac
     1261 cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg tggttgtcag
     1321 ttcattaatt ggtggtttaa ttaacgcctt tattcttgat ctccccatca ataccgcgct
     1381 ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga ccgaatcttt
     1441 tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgaac tgattgctat
     1501 tatgttgatc cctgggctga ttcgccgcag ccgctctact gcactgggct tatgcggtgc
     1561 cacatcaatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg atatggtccc
     1621 ggcggcaatt gttcacggtt ttattcttag cctgttagtg ccgatcctca tcgccttttt
     1681 ctctgcgtaa tacctctctg gcggtagatc cctgccgcca aaattgcgct aaatcaatct
     1741 cccttaaagt tgcatgaaaa atccctttta tccccgcgtt aagcgtctta accttaaaca
     1801 tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg tgaacaaact
     1861 atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg taaaacggcg
     1921 gaaacttctg accttcagga tttactcatc gcggcgctgc aagggctttc tgcctgggcg
     1981 gtaaaagcgt gtgaatacgg catcatcaac cacgatgttg acagcttcgc gccacgtgcc
     2041 tttttctcaa ccctgaccaa cgttaacttc gattctccgc gtattgtcgg ctacgctcgt
     2101 gaagcgattg ccttgcgcga ggcgctgaaa gcacaatgcc tggctgtaga tgccaacgcg
     2161 cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg atgatctcgg cgagctgcaa
     2221 cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga aaacattctc
     2281 ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat ggaacacgcg
     2341 catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat catggcgtgg
     2401 ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga aatcggccag
     2461 atgaacttca aagtgatgag cattctggat gcaggcgaaa ccggtaaata cggtcacccg
     2521 acgccaaccc aggtcaacgt caaagcgacg gcgggtaaat gcattctgat ttccggtcac
     2581 gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt taatgtctac
     2641 acccacggcg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt caagcatctg
     2701 gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg tttccctggc
     2761 cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta tgacgatcgt
     2821 atctggaccc gcagcattgt tggctggcct ggcgtgcgtc atctggacgg tgatgatttc
     2881 tctgcggtta tcacccaggc gcaacagatg gcaggcttcc cgtacagcga aattccgcac
     2941 cttatcaccg tgggttttgg tcgccagacg ctgcttggcg ctgctgatac gctgattgat
     3001 ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga cggcgcacgc
     3061 ggcgagcgcc actacttcac cgatttcgcc accagcgtgc cggatgactg cctgatcctg
     3121 accctcgcct gtggtaaata tcgctttaac aaacttgagt ttggcgatat cgaaggtctg
     3181 ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat tctggctgtc
     3241 actctggcag agaaactggg ctgtggtgtg aacgatctgc cgctgtcgct ggtgctctcc
     3301 tggtttgaac agaaagcgat cgtcattctg ctgacgctgc tttctctggg cgtgaaaaat
     3361 atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc ggtgctgaac
     3421 gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca actgttgagc
     3481 gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg atgcaggttc
     3541 atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc cacgattact
     3601 acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg aaactcagcg gaaacgctgc
     3661 gtgcttacac catttcctcc acgccaggcg tgagtgaata tatcaccctg accgtgcggc
     3721 ggattgatga cggtgtcggc tcccagtggc tgacgcgcga tgtaaaacgc ggtgattatc
     3781 tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa gataaattcc
     3841 tgttgctggc ggcaggctgc ggcgtcacgc cgattatgtc gatgcgtcgc tggcttgcga
     3901 agaaccgtcc acaggccgat gtgcgggtga tctacaacgt gcgtacgccg caggatgtta
     3961 ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcagaaaat aacgttaccg
     4021 aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtacct gacttagctt
     4081 cacgtaccgt gatgacctgc ggcccggctc cgtatatgga ttgggtagag caggaagtga
     4141 aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta gcagaagcgg
     4201 cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac gccccggttg
     4261 gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgct gcctgccgtg
     4321 cgggtgtttg cggctgctgt aagacaaaag tggtttccgg tgaatatacg gtgagcagta
     4381 caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc tcctgccatc
     4441 cgcaggggga tttggttctc gcataatcgc cttatgcccg atgatattcc tttcatcggg
     4501 ctatttaacc gttagtgcct cctttctctc ccatcccttc cccctccgtc agatgaacta
     4561 aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg gttgcagctt
     4621 atatcgccaa aacactcgaa tcggcagggg tgaaacgcat ctggggagtc acaggcgact
     4681 ctctgaacgg tcttagtgac agtcttaatc gcatgggcac catcgagtgg atgtccaccc
     4741 gccacgaaga agtggcggcc tttgccgctg gcgctgaagc acaacttagc ggagaactgg
     4801 cggtctgcgc cggatcgtgc ggccccggca acctgcactt aatcaacggc ctgttcgatt
     4861 gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc agcgaaattg
     4921 gcagcggcta tttccaggaa acccacccac aagagctatt ccgcgaatgt agtcactatt
     4981 gcgagctggt ttccagcccg gagcagatcc cacaagtact ggcgattgcc atgcgcaaag
     5041 cggtgcttaa ccgtggcgtt tcggttgtcg tgttaccagg cgacgtggcg ttaaaacctg
     5101 cgccagaagg ggcaaccacg cactggtatc atgcgccaca accagtcgtg acgccggaag
     5161 aagaagagtt acgcaaactg gcgcaactgc tgcgttattc cagcaatatc gccctgatgt
     5221 gtggcagcgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg aaaattaaag
     5281 cgcctattgt tcatgccctg cgcggtaaag aacatgtcga atacgataat ccgtatgatg
     5341 ttggaatgac cgggttaatc ggcttctcgt caggtttcca taccatgatg aacgccgaca
     5401 cgttagtgct actcggcacg caatttccct accgcgcctt ctacccgacc gatgccaaaa
     5461 tcattcagat tgatatcaac ccagccagca tcggcgctca cagcaaggtg gatatggcac
     5521 tggtcggcga tatcaagtcg actctgcgtg cattgcttcc attggtggaa gaaaaagccg
     5581 atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa gggctggacg
     5641 atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag caaattagtc
     5701 attttgccgc cgatgacgct attttcacct gtgacgttgg tacgccaacg gtgtgggcgg
     5761 cacgttatct aaaaatgaac ggcaagcgtc gcctgttagg ttcgtttaac cacggttcga
     5821 tggctaacgc catgccgcag gcgctgggtg cgcaggcgac agagccagaa cgtcaggtgg
     5881 tcgccatgtg cggcgatggc ggttttagca tgttgatggg cgatttcctc tcagtagtgc
     5941 agatgaaact gccagtgaaa attgtcgtct ttaacaacag cgtgctgggc tttgtggcga
     6001 tggagatgaa agctggtggc tatttgactg acggcaccga actacacgac acaaactttg
     6061 cccgcattgc cgaagcgtgc ggcattacgg gtatccgtgt agaaaaagcg tctgaagttg
     6121 atgaagccct gcaacgcgcc ttctccatcg acggtccggt gctggtggat gtggtggtcg
     6181 ccaaagaaga gttagccatt ccaccgcaga tcaaactcga acaggccaaa ggatttagct
     6241 tgtatatgtt gcgcgcaatc atcagcggac gcggcgatga agtgatcgaa ctggcgaaaa
     6301 cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat gccatgattg
     6361 atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca atgatggccg
     6421 ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg caggactacg
     6481 ccgcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc caggctaacc
     6541 tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt caggccgcgc
     6601 ataattatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa ccgcaaccca
     6661 tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa atcaaacccg
     6721 acgatatcca tttcgcccgc accaaattac tcagtctaga aaacacccac aacggcaaag
     6781 tgctgccgcg tgaatacctg aaagaagcat gggaatttac ccgcaagcgc aatctggcgc
     6841 tgcatgttga cggtgcgcgc atctttaatg ccgtggtggc ttacggctgc gaactgaaag
     6901 agatcacgca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt gggacgccag
     6961 tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccatccgc tggcggaaaa
     7021 tggccggtgg cgggatgcgt cagtccggga ttctggctgc cgccgggatg tatgccctga
     7081 aaaataacgt cgcacggttg caggaagatc acgacaacgc cgcctggatg gcggagcaac
     7141 tgcgtgaagc aggcgcggat gtgatgcgtc aggacactaa tatgctgttt gttcgcgtcg
     7201 gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg ctgattaacg
     7261 cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgaacaa ctggcggaag
     7321 tcgccgccca ctggcgcgca ttcctggcgc gttaaggaga gagacgtgcc gcaacgcatt
     7381 ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgcacact cagccagcaa
     7441 gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct gcaactggca
     7501 aacgtcagtt gccataaagt cgatctcagc tggccggata accttccggc cctgttgcag
     7561 gatatcgata ccgtctattt tctggtgcac agcatgggcg aaggcggcga ttttatcgct
     7621 caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtacc agttaagcaa
     7681 ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggatca tctgcgtgct
     7741 cgtcaggcta cggcggacat tcttcgtgaa gcgaatgtac ctgtgaccga acttcgggcc
     7801 ggaattatcg ttggcgcagg ttcagcggcg ttcgaagtca tgcgcgatat ggtctacaac
     7861 ctgccagtgt taacgccgcc acgctgggta cgttcacgca ccacgcccat cgcgctggaa
     7921 aacttgctgc actatctggt ggcgttgtta gatcatccgg ccagcgaaca ccgcatcttc
     7981 gaagccgccg gaccagaggt gctcagttat cagcaacagt ttgaacattt tatggcggtg
     8041 agcggtaagc gccgctggtt gatccccatc cccctcccca cccgctggat ttcggtgtgg
     8101 tttctcaatg tgattacttc cgtaccgccc accaccgcca gggcgttgat tcaggggctg
     8161 aaacacgatc tgctggcgga tgataccgcg ctacgtgcac tcatcccaca acggctgatc
     8221 gctttcgatg acgcggtacg tagcacgttg aaagaggagg aaaaactggt caactccagc
     8281 gactggggct acgacgctca ggcctttgcc cgctggcgac cggagtacgg ttattttgcc
     8341 aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg ctgctttatg gcaggtagtg
     8401 aaccaaatcg gcggtaaaga gcgttatttc tttggcaata ttttgtggca gacacgggcg
     8461 ttgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg cgaatattta
     8521 cagactggcg atgcggtgga tagctggaaa gtgattgtcg ttgaaccgga aaaacaactt
     8581 acgttgttat ttggcatgaa agcgtcgggg ctgggacgac tgtgttttag cctggaagat
     8641 aaaggcgact atcgtactat cgatgtccgc gctttctggc atccgcacgg tatgccgggg
     8701 ctgttttact ggttattgat gatccccgcg catctgttta tttttcgcgg aatggcaaaa
     8761 caaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc gttattcttt
     8821 catgtttccc attgcatcat gggggaaatc acggaagaat gcgcacgtca ttttctcaac
     8881 aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta ggtcgaaacg
     8941 cggtagagtt tttatgccag aaaggcatca gcgtgcgagc gaccggtcgc aacgaggcaa
     9001 tgggcaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg accgagctgg
     9061 tttcatcaca agctaaagtg atgctcgcgg gcattgatac gctgtggcac tgctccagct
     9121 ttacctcacc ctgggggaca caacaggctt tcgatctggc taacgttcgc gccactcgcc
     9181 gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc tcttccccct
     9241 ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc cctcaccgct
     9301 tcgccaacga gtttgcccgc agcaaagcag ccagcgaaga agtgatcaat atgctttcgc
     9361 aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc ggaccgcacg
     9421 ataaagtctt tattccccgt ctggcgcata tgatgcacca ctacggcagt attctgttac
     9481 cgcatggcgg cagtgcgctg gtcgatatga cctactatga aaataccgtg cacgcaatgt
     9541 ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac aatatcacca
     9601 acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgacgag ttgaatattg
     9661 actgtcgtat tcgttccgtc ccctacccga tgctggatat gatcgcccgc agcatggagc
     9721 gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc tccaagttga
     9781 attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat cagccgatca
     9841 tcaccctaga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac ggaaaactgc
     9901 cgcgctaatc ctgcccgtat ttctccagca atgcttcggc aatcgcctga gtttctgcat
     9961 ccgcttcgcc gttatataac gtcgggcgga aatgcatctg gaatgccata atcacgcgcc
    10021 gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc aaaagctcca
    10081 gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa aagttaaccc
    10141 gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag ggaaataatg
    10201 gccccggatc gtctttgcgc tgcggtgcga tatccgcatg tgccactacg ttttccggct
    10261 tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga atctgtgtcg
    10321 gttcaaacgg ggcaaaatat ttcactccgg ccgatttttg ccagccacgg ttttccagct
    10381 caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta atccccgcat
    10441 gccaggccag ttcttgttcc ggcaccagtt gccagatgcg cggtttaccg ttgtatcgcg
    10501 gtggtaccgc agggaccaga taatgcgagc tgacctgctt atcggtcagt gtcgccagcg
    10561 agctatcaaa atcatctgcg gtgtagtgga tcaccagcac tttaatgcgc ggatacgccg
    10621 cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg cctttttcgc
    10681 ctgcacaccc tgccaataac agagcggcag cgaccagcca gaagactctt ctcatcgacg
    10741 cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt gcccgaccgt
    10801 ttcgtagtcg atatcaatac cgacgacggc atcggccccc agcgcccgcg cctgggagcc
    10861 taattcctca aaggcgatct cccgtgcttt acgcagttct ttttcatacg caccggaacg
    10921 tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg cacctaaaat
    10981 cgcttcgccg gtcaccacac cgcaatattc aacgatggtc tggccttcca gagttggggt
    11041 tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct aaagcattat
    11101 cgaactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc atctcattgt
    11161 gtcaaaaaca cccacttaag tctttacgat tatccgttgc ataacccgat attggtttca
    11221 ttatagggta ccgcttacct gtcttattat gttgcaccaa caacattaat gagcggaaag
    11281 aggcaactta tattgtaaat gactcctaaa gtcttcccca tcttgtgact gaggtatata
    11341 cctctggatt gctttttggt cagacgccgc aaaacaagaa agtaataaga tctcaatgct
    11401 tttgtcatta atataagcga agatcattct tgcaaattcg taaataaacg cccgttcctg
    11461 agcgcttaat agtttaatgc aatatttatt cttaagagag aaaataattt caaggaaagc
    11521 atccgcagca accttcacgt tattcccggc gctcatatta tatatatccc ttgcacagat
    11581 agagtacaga gatagtaaac agccatacaa ataatcattt attaactctt ttgtccattt
    11641 tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa aaagacattt
    11701 gctgaaatta actcttatca tatcaacatt aaaaaaatgg caattaataa attttaaaga
    11761 atgagtattt acgttgttaa ttttaacaga tctaaacgaa acatttttga atacattatt
    11821 tattatattg ctatttgtta ttttaacatc acctaaactg ccgttttcaa aacaacattt
    11881 tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta tgttttgctt
    11941 tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat tgagtagtgt
    12001 tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg gaacaggcag
    12061 agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc cctttgtcat
    12121 atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag gaaacagatt
    12181 aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat gaacaggttt
    12241 tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat ctaacgcagt
    12301 ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg tattatcact
    12361 gtcataaaaa gtgttcatca atgcgatatt atttgttaca gcgtcttttt cgatattctc
    12421 actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct ttataaagga
    12481 tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa tcacattcgc
    12541 aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg taaaatgctt
    12601 gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact gttgtctttc
    12661 aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac ccgcctttat
    12721 ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct cgaaagcatt
    12781 actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga gcaacacatc
    12841 attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt agatggtatc
    12901 ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt ccctcagtga
    12961 atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg cttgatcaga
    13021 tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg aactcctcgt
    13081 gctttataat tataaaagca caacgccttt tatattcata ttaattaacg agatactgaa
    13141 acaacttaat ttattcttcg ctaaaattca ttag
//
LOCUS       DAZRVY010000113        13114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZRVY010000113 DAZRVY010000000
VERSION     DAZRVY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.113"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            142..441
                     /locus_tag="ACHXOX_003254"
     CDS             142..441
                     /locus_tag="ACHXOX_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006645.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(488..1375)
                     /gene="rarD"
                     /locus_tag="ACHXOX_003255"
     CDS             complement(488..1375)
                     /gene="rarD"
                     /locus_tag="ACHXOX_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR7006646.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1427..1894)
                     /gene="yigI"
                     /locus_tag="ACHXOX_003256"
     CDS             complement(1427..1894)
                     /gene="yigI"
                     /locus_tag="ACHXOX_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR7006647.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2059..2928
                     /gene="pldA"
                     /locus_tag="ACHXOX_003257"
     CDS             2059..2928
                     /gene="pldA"
                     /locus_tag="ACHXOX_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR7006648.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3061..4890
                     /gene="recQ"
                     /locus_tag="ACHXOX_003258"
     CDS             3061..4890
                     /gene="recQ"
                     /locus_tag="ACHXOX_003258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR7006649.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4954..5571
                     /gene="rhtC"
                     /locus_tag="ACHXOX_003259"
     CDS             4954..5571
                     /gene="rhtC"
                     /locus_tag="ACHXOX_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR7006650.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5633..6253)
                     /gene="rhtB"
                     /locus_tag="ACHXOX_003260"
     CDS             complement(5633..6253)
                     /gene="rhtB"
                     /locus_tag="ACHXOX_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR7006651.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6364..7386
                     /gene="pldB"
                     /locus_tag="ACHXOX_003261"
     CDS             6364..7386
                     /gene="pldB"
                     /locus_tag="ACHXOX_003261"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR7006652.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7394..8194
                     /gene="yigL"
                     /locus_tag="ACHXOX_003262"
     CDS             7394..8194
                     /gene="yigL"
                     /locus_tag="ACHXOX_003262"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR7006653.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8270..9169
                     /gene="bioP"
                     /locus_tag="ACHXOX_003263"
     CDS             8270..9169
                     /gene="bioP"
                     /locus_tag="ACHXOX_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR7006654.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9057..10010)
                     /gene="metR"
                     /locus_tag="ACHXOX_003264"
     CDS             complement(9057..10010)
                     /gene="metR"
                     /locus_tag="ACHXOX_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR7006655.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10246..12507
                     /gene="metE"
                     /locus_tag="ACHXOX_003265"
     CDS             10246..12507
                     /gene="metE"
                     /locus_tag="ACHXOX_003265"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR7006656.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12629..12751)
                     /locus_tag="ACHXOX_003266"
     CDS             complement(12629..12751)
                     /locus_tag="ACHXOX_003266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006657.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12792..13037)
                     /locus_tag="ACHXOX_003267"
     CDS             complement(12792..13037)
                     /locus_tag="ACHXOX_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR7006658.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3106 a         3257 c         3492 g         3259 t
ORIGIN      
        1 caatatagca gtctttgcat tgataactat acagtagtaa tttagagtgt gtaagttttt
       61 attgatgggg tatattttaa ttaaatataa ttgaaatgta tttttattct ataaacaata
      121 tcttgggtga atggattcta tatgggaaat ggtaactttt ggcagttgtt cttgggtaaa
      181 gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt tattgtggtg ggctattacc
      241 atccttgttc tatttatttt aagcctgact acgacattaa atttaagaag tataggtata
      301 gggttaagtt ttgggttttg tcaaatcctt ggtagatata cgatgtctct aggatggagt
      361 aagtactggg gagttttggg ggtatttcct gtaacaaata ttgttctttt tcttatctta
      421 atttttttca aaaaaagata aataagtcag tgggtaggct ctttaaacgg cctacccatg
      481 ttaattctta actttttcta cgctgcgtat aaatcgcatc catcacaaaa attgccagcg
      541 ccacccaaat aaaggcgaaa gtcaccatct tatcggcacc cggtttttca ccataaaacg
      601 tcacagccag caggaacatc agcgtcgggc caatgtactg gaaaaagcct aacgttgaga
      661 gacgcaagcg cgtggcagcg gcggtaaaac acaacagcgg tacggtagtg acaataccgg
      721 cggcgatcag cagtaaattc agcgacatcg ggttttgccc catatggctg gttgagctgt
      781 cggcaatagc aaacaggtaa attgccgcca cgggcagcag ccacatggtt tcgattaaca
      841 tgccggtttg cgcttcaacg gcaatcttct tgcgtaccag accgtagaag gcaaaactaa
      901 atgccagtcc cagcgcgata ataggtagcg aaccaaaagt ccacagctgg actaacacgc
      961 cacatatcgc cagaatcacc gccagccatt gcatccggcg gaatcgctcg ccgaggaaaa
     1021 tcatccccag cacaatgttc accagcgggt taataaagta accaaggctc gcttccagca
     1081 tatggtgatt gttcaccgcc cagataaaca gtagccagtt gccaccaatc agcacggcag
     1141 agactgccag cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa taggaccact
     1201 ggcggcaaat gctcatcagc accaccataa agaaaaacga ccagatcacg cgatgcgtca
     1261 ggatttcatc ggcgggcacg tagtaaatca acttgaagta cgctggcgct ataccccaaa
     1321 taaaataagc ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca tccatcggga
     1381 atactcattt ttaattggta acagcagttt acctgctttt atgtcttcaa cctaccatat
     1441 aggtggcggt ggcactggca atataaagct gttcttcatt gtgtaattca acgcgggcga
     1501 cggcgacttt attgcctgca cgcaacaggc tactggtagc agtaaaacgc tcgcccctgc
     1561 ctgggcgcag ataatcaacg cgaagatcaa tggtccccat ccgcgatagc cgctggcgta
     1621 gttcatcttc actgatggtt tcgtggcggg ttaaggtact tcccacgcac accagaccgg
     1681 cggcgacatc cagcgccgac gcaatgaccc cgccgtgcaa aatgctttgc gcccagttgc
     1741 ccaccatcat tggctgattt ttaaaggcca gctgtgcgaa ctctttttcg taacgctcca
     1801 gttccatccc caatgcgcgg ttaaatggca tgtgataaac aaacatctca cccactaatt
     1861 tcagggcttg ttcagcggtc agtacggcag acatatcatc cttacacttc attggttaat
     1921 gaaatgttga ttttatgctt ctttgttgtt ggtttctact ttaggaaggg ataactaacg
     1981 gctatggagt taagtatgta aaatagcccg cagaaaaata ttcaccttat caataattcg
     2041 ttacggagaa cacgaccgat gcggactctg cagggctggt tgttgccggt gtttatgttg
     2101 cctatggcag tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc gccagcggtg
     2161 cgtggcagta ttatcgccaa tatgctgcag gagcatgaca atccgttcac gctctatcct
     2221 tatgacacca actacctcat ttacacccaa accagcgatc tgaataaaga agcgattgcc
     2281 agttacgact gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt gagcctggca
     2341 tttccgctgt ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc ttatacgcaa
     2401 aaatcctggt ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga aaccaactac
     2461 gaaccgcaat tgttcctcgg ttttgccacc gattactgtt ttgcaggttg gacgctgcgc
     2521 gatgtggaga tggggtataa ccacgactct aacgggcgtt ccgacccgac ctcccgcagc
     2581 tggaaccgcc tttatactcg cctgatggca gaaaacggta actggctggt agaagtgaag
     2641 ccgtggtatg tggtgggtaa tactgacgat aacccggata tcaccaaata tatgggttac
     2701 taccagctta aaatcggcta tcacctcggt gatgcggtgt tgagtgcgaa aggacagtac
     2761 aactggaata ccggttacgg cggcgcggag ttaggcttaa gttacccgat caccaaacat
     2821 gtgcgccttt atactcaggt ttacagcggc tatggcgaat cgctcatcga ctataacttc
     2881 aaccagaccc gtgtcggtgt aggagttatg ctaaacgact tgttttgatg aacggttgag
     2941 tggttggcaa atctggaacc cagcatccag gattaccctc tcagagacta aaagcattgc
     3001 agtttctcgc gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat cggggtgaat
     3061 gtggcgcagg cggaagtgtt gaatctggag tccggagcta aacaggtttt acaagaaacc
     3121 tttggctacc aacagtttcg ccccggccag gaagaaatta tcgacactgt gctttccggt
     3181 cgcgattgcc tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta tcaaattcct
     3241 gccttattgc taaacggcct taccgtggtt gtttcaccgc tgatttcgtt gatgaaagat
     3301 caggtggatc aactgcaagc caacggcgtg gcggcggcgt gccttaactc gacgcaaacc
     3361 cgcgagcagc aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg tctgctttat
     3421 atcgccccgg aacgcctgat gctggataac tttcttgagc atctggcgca ctggaatccg
     3481 gtgttattag ccgttgatga agcgcactgt atctcccaat ggggccacga tttccgcccg
     3541 gaatatgccg cgcttggtca gttacgccag cgtttcccga ccctgccgtt tatggcgctg
     3601 actgccacag ccgacgacac cacgcgccag gatatcgtgc gcttgctggg gctgaacgat
     3661 ccgctgattc aaatcagcag ttttgaccgt ccgaatattc gctacatgct gatggagaaa
     3721 ttcaaaccgc tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa gtccggcatt
     3781 atctactgca acagccgggc gaaagtagaa gacaccgctg cgcgcctgca aagcaagggt
     3841 attagcgcgg cggcctatca tgccgggctg gaaaataatg tccgcgccga cgtgcaggag
     3901 aaattccagc gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg catgggcatc
     3961 aataaaccaa acgttcgctt cgtggtccac tttgatattc cgcgcaatat cgaatcctat
     4021 tatcaggaaa ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat gctgttttac
     4081 gatccggctg atatggcgtg gctgcgccgt tgtctggaag agaagccgca ggggcagttg
     4141 caggatattg agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc gcaaacttgc
     4201 cgtcgtctgg tattgctgaa ctattttggc gaagggcgtc aggagccgtg cgggaactgc
     4261 gatatctgcc tcgatccgcc gaaacagtac gacggttcaa ccgatgctca gattgccctt
     4321 tccaccattg gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga agtgattcgt
     4381 ggtgctaata accagcgtat ccgcgactat ggtcatgaca aactgaaagt ctatggcatg
     4441 ggccgtgata aaagccatga acattgggtg agcgtgatcc gccagctgat tcacctcggc
     4501 ctggtgacgc aaaatattgc ccagcattct gccctacaac tgacagaggc cgcgcgcccg
     4561 gtgctgcgcg gcgaatcctc tttgcaactt gccgtgccgc gtatcgtggc gctcaaaccg
     4621 aaagcgatgc agaaatcgtt cggcggcaac tatgatcgca aactgttcgc caaattacgc
     4681 aaactgcgta aatcgattgc cgatgaaagt aatgtcccgc cgtacgtggt gtttaacgac
     4741 gcaaccttga ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat gctcagcgtt
     4801 aacggcgttg ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc gctgattcgt
     4861 gcgcatgtcg atggcgacga cgaagagtag tcagcagcat aaaaaagtgc caggatgaag
     4921 actccgtaaa cgtttccccc gcgagtcaaa tgtatgttga tgttatttct caccgtcgcc
     4981 atggtgcaca ttgtggcgct tatgagcccc ggtcccgatt tcttttttgt ctctcagacc
     5041 gctgtcagtc gttcccgtaa agaagcgatg atgggcgtgc tgggcattac ctgcggtgta
     5101 atggtttggg ctgggattgc gttgcttggc ctgcatttga ttatcgaaaa aatggcctgg
     5161 ctgcatacgc ttatggttgg cggtggcctg tatctctgct ggatgggtta ccagatgctg
     5221 cgcggcgcgc tgaaaaaaga ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa
     5281 agtgggcgca gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc
     5341 tactttggct cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg
     5401 ggcatttttg cgctgatcat tgtcgaaacg ctggcgtggt ttatcgtcgt tgccagcctg
     5461 tttgccctgc cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt
     5521 gccggggcgt tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg
     5581 cgtcttcaga gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat
     5641 gcctcgccga tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca
     5701 gcgccttcat ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt
     5761 aaccgatcat cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca
     5821 tcagttgcgg ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac
     5881 ttttgggatt ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag
     5941 tagaggccag cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc
     6001 aaatcaagta agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa
     6061 atagcgtccc caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc
     6121 cagcaataga cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga
     6181 ttgcaccaga gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc
     6241 attctaaggt catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc
     6301 gcagcgttgt gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac
     6361 ccgatgtttc agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc
     6421 atgggaccgc tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat
     6481 gacattccgg tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc
     6541 tgcccggggc gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat
     6601 ttggggtttg atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta
     6661 gccgatccgc atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca
     6721 ttctggcagc aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg
     6781 atgggcggtg cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt
     6841 gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag
     6901 atcctcaact gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg
     6961 cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga
     7021 cgtaacttac gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat
     7081 tgggtacgcg aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc
     7141 acgccaacgc ttctcttgca ggctgaagag gaacgcgtgg tggataaccg catgcatgac
     7201 cgtttttgtg aactccgcac cgccgcgggc catcctgtcg aaggaggacg accgttggta
     7261 attaaaggtg cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc
     7321 cacgccattg ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag
     7381 gtttaaattt cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc
     7441 cgaccatacg ttatcccctt acgccaaaga aaccctgaag ctgctcaccg cgcgcggcat
     7501 caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct
     7561 ggagatcaag tcgtacatga tcacctccaa cggcgcgcgc gttcacgatc tggatggcaa
     7621 tctgattttt gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa
     7681 cgacaatctg gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca
     7741 tcgcccggaa gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc
     7801 tggattactg gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca
     7861 actgctgccg ctggagcagg cgattaacgc tcgttggggc gatcgcgtca acgttagttt
     7921 ctctacctta acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga
     7981 agcggtggcg aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat
     8041 gaacgatgcc gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca
     8101 gcgtctgaaa gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt
     8161 gccgcattat ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa
     8221 cctgatactt cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact
     8281 tatcatcacc acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg
     8341 gcacgtcgat agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct
     8401 gccgtttctg cgtacccgtg gcaatagcct gaaaacggtc ggcctgtata tgctggtggg
     8461 cgcgatgcag ctcggcgtga tgtatatgct gagtttccgc gcttatctct acctgacggt
     8521 ctccgaattg ttgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat
     8581 catgagtaag cgccgtctgc gctggggcta tgcttttagc gccttgctgg cggtgattgg
     8641 tgccgggatt attcgctatg atcaggttac cgaccatttc tggactggct tgttgctggt
     8701 gcaactctcc aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac
     8761 tcgcccgatg ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc
     8821 ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg
     8881 gggcattctg gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta
     8941 cggcgcgacg caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc
     9001 agggctgctg gtaaacctgg ctatctggca ccaacagccg cactggccaa cgttcattac
     9061 aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc
     9121 ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta
     9181 atcggctgac gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg
     9241 cccagggttt tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt
     9301 agcgcggcaa tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg
     9361 cttttcagtg acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga
     9421 ctacgctgca ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc
     9481 gttttcgccg ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc
     9541 ggcgaataat gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg
     9601 ccctgttgca acgcaggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc
     9661 tgcggccagt tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg
     9721 cactcaatgg caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg
     9781 ctgatttgcg gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag
     9841 cgtagcggct ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta
     9901 aactggtgag acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag
     9961 ccgcagttcc gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt
    10021 cacttcggca tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg
    10081 taaacatctg gacggctaaa atccttcgtc ttttaaattt atggtgcgtt ggctgcgttt
    10141 ctccaccccg gtcacttact tcagtaagct cccggggatg aataaacttg ccgccttccc
    10201 taaattcaaa atccatagga tttacatata attagaggaa gaaaaatgac aatattgaat
    10261 cacaccctcg gtttccctcg cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt
    10321 tattgggcgg ggaactccac gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct
    10381 cgtcactggg atcaacaaaa gcaagcgggt atcgacctgc tgccggtggg cgattttgcc
    10441 tggtacgatc atgtactgac caccagtttg ctgctgggta atgttccgcc acgtcatcag
    10501 aacaaagacg gttcggtaga tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg
    10561 actggcgaac ctgcggcggc agcggaaatg accaaatggt ttaacaccaa ctatcactac
    10621 atggtgccgg agttcgttaa aggccaacag ttcaaactga cctggacgca gctgctggag
    10681 gaagtggacg aggcgctggc gctgggccac aacgttaagc ctgtgctgct ggggccggtt
    10741 acttacctgt ggctggggaa agtgaaaggt gaacagtttg accgccttag cctgctgaac
    10801 gacattctgc cggtttatca gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg
    10861 gtacagattg atgaacccgc gctggtactg gaactaccac aggcgtggct ggacgcatac
    10921 aaacccgctt acgacgcgct ccagggacag gtgaaactgc tgctgaccac ctattttgaa
    10981 ggcgtaacgc caaatctcga cacgattact gcgctgcctg ttcagggtct gcatgttgat
    11041 ctcgtacatg gtaaagatga cgttgctgaa ctgcacaagc gcctgccttc tgactggttg
    11101 ctgtctgcgg gtctgatcaa tggtcgtaac gtctggcgcg ccgatcttac cgagaaatat
    11161 gcgcaaatta aggacattgt cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg
    11221 ctgcacagcc ccatcgacct gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg
    11281 tttgccttcg ccctacaaaa atgccatgaa ctagcactgc tgcgcgatgc gctgaacagt
    11341 ggtgacacgg cagctctggc agagtggagc gccccaattc aggcgcgtcg tcactctact
    11401 cgtgtacata atccggcggt agaaaagcgt ctggcggcga tcaccgccca ggacagccag
    11461 cgtgcgaatg tctatgaagt gcgtgctgaa gcccagcgtg cacgttttaa actgccagcg
    11521 tggccgacca ccacgattgg ttccttcccg caaaccacgg aaattcgtac cctgcgtctg
    11581 gatttcaaaa agggcaatct cgacgccaat aactaccgca cgggcattgc ggaacatatc
    11641 aggcaggcca ttgttgaaca ggaacgtttg ggactggatg tgctggtaca tggcgaggcc
    11701 gagcgtaatg acatggtgga atactttggc gagcacctcg acgggtttgt ctttacgcaa
    11761 aacggttggg tacagagcta cggttcccgc tgcgtgaagc caccgattgt tattggtgac
    11821 gttagtcgcc cggcaccgat taccgtggag tgggcgaagt atgcgcaatc tctgactgat
    11881 aaaccggtga aagggatgct gactggcccg gtgactattc tctgctggtc gttcccgcgt
    11941 gaagatgtca gccgtgaaac catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg
    12001 gccgatctgg aagccgctgg aattggcatc atccagattg acgaaccggc tctgcgcgaa
    12061 ggtttaccgc tgcgtcgtag cgactgggat gcgtatctcc agtggggcgt agaggccttc
    12121 cgtatcaacg ccgccgtggc gaaagatgac acacaaatcc acactcacat gtgttattgc
    12181 gagttcaacg acatcatgga ttcgattgcg gcactggacg cagacgtcat caccatcgaa
    12241 acctcgcgtt ctgacatgga gttgctggag tcgttcgaag agtttgatta tccgaatgaa
    12301 atcggtccag gcgtctatga cattcactcg ccaaacgtac cgagcgtgga atggattgaa
    12361 gccttgctga agaaagcggc aaaacgcatt ccggcagagc gcctgtgggt taacccggac
    12421 tgtggcctga aaacgcgcgg ctggccagaa acccgcgcgg cactggcgaa catggtgcag
    12481 gcggcgcaga atttgcgtcg gggatgatga atattaacga gtcactgtaa attgcagtac
    12541 gatgaaagag gcaaaactgc ctgatactag gcctacataa tttatgcaat ttattgaatt
    12601 tttgggtttt gttggccgga caaggcgttc acgccgcatc cggcatgaac aacgcgcact
    12661 ttgccaataa tctgatggct tccatcaccg gaagccattt tgctatctgc gcccgttcag
    12721 atacagaaat aaccgaatca tttttacgca aaatataatc ctgagcgatt tgttcagtaa
    12781 tcaacgctac gttatttatt ctcacttttc acgaaattgt atcccttttc agtaacgtgg
    12841 tagtgaggca tagacttatg tccgctatcc tcaataataa ccaaaccctc gttagctaat
    12901 ttgttaacaa tgttaacaac attaccgaat cctggaattt gccttatgga tagtgtgcga
    12961 tcgagattca tccagttcca gtttatatct ttctcagcca taattttcag tacgttaatt
    13021 tcagtttcag ttatcatagt caagtgcatc ctggtggtat aagtcattaa gttctccctt
    13081 aagtttataa tcttctagag tagcagtatg cgta
//
LOCUS       DAZRVY010000114        12998 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZRVY010000114 DAZRVY010000000
VERSION     DAZRVY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.114"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHXOX_003268"
     CDS             <1..191
                     /locus_tag="ACHXOX_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006659.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHXOX_003269"
     CDS             191..1393
                     /locus_tag="ACHXOX_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR7006660.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXOX_003270"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXOX_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR7006661.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHXOX_003271"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHXOX_003271"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR7006662.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXOX_003272"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXOX_003272"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR7006663.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXOX_003273"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXOX_003273"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR7006664.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXOX_003274"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXOX_003274"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR7006665.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXOX_003275"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXOX_003275"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR7006666.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXOX_003276"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXOX_003276"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR7006667.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZRVY010000115        12563 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZRVY010000115 DAZRVY010000000
VERSION     DAZRVY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.115"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHXOX_003277"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHXOX_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR7006668.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHXOX_003278"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHXOX_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR7006669.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHXOX_003279"
     CDS             1797..1970
                     /locus_tag="ACHXOX_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006670.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHXOX_003280"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHXOX_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR7006671.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHXOX_003281"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHXOX_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR7006672.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHXOX_003282"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHXOX_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR7006673.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHXOX_003283"
     CDS             complement(4268..5191)
                     /locus_tag="ACHXOX_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7006674.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHXOX_003284"
     CDS             5408..6709
                     /locus_tag="ACHXOX_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7006675.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHXOX_003285"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHXOX_003285"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR7006676.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHXOX_003286"
     CDS             8771..8995
                     /locus_tag="ACHXOX_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR7006677.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHXOX_003287"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHXOX_003287"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR7006678.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHXOX_003288"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHXOX_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR7006679.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHXOX_003289"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHXOX_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR7006680.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHXOX_003290"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHXOX_003290"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR7006681.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZRVY010000116        12413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZRVY010000116 DAZRVY010000000
VERSION     DAZRVY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.116"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..775
                     /locus_tag="ACHXOX_003291"
                     /pseudo
     CDS             1..775
                     /locus_tag="ACHXOX_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            1098..2486
                     /gene="narU"
                     /locus_tag="ACHXOX_003292"
     CDS             1098..2486
                     /gene="narU"
                     /locus_tag="ACHXOX_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR7006682.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2568..6308
                     /gene="narZ"
                     /locus_tag="ACHXOX_003293"
     CDS             2568..6308
                     /gene="narZ"
                     /locus_tag="ACHXOX_003293"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR7006683.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6305..7849
                     /gene="narH"
                     /locus_tag="ACHXOX_003294"
     CDS             6305..7849
                     /gene="narH"
                     /locus_tag="ACHXOX_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR7006684.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7849..8544
                     /gene="narW"
                     /locus_tag="ACHXOX_003295"
     CDS             7849..8544
                     /gene="narW"
                     /locus_tag="ACHXOX_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR7006685.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8541..9221
                     /gene="narI"
                     /locus_tag="ACHXOX_003296"
     CDS             8541..9221
                     /gene="narI"
                     /locus_tag="ACHXOX_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR7006686.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9300..10193
                     /locus_tag="ACHXOX_003297"
     CDS             9300..10193
                     /locus_tag="ACHXOX_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR7006687.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="ACHXOX_003298"
     CDS             complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="ACHXOX_003298"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR7006688.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11307..11876
                     /locus_tag="ACHXOX_003299"
     CDS             11307..11876
                     /locus_tag="ACHXOX_003299"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR7006689.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="ACHXOX_003300"
     CDS             complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="ACHXOX_003300"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR7006690.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(12207..>12413)
                     /locus_tag="ACHXOX_003301"
     CDS             complement(12207..>12413)
                     /locus_tag="ACHXOX_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7006691.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         3006 a         3162 c         3166 g         3079 t
ORIGIN      
        1 tttgtgcaat tagaatcttg cgaatgtctg caatacctga atctcagcca taatcaatta
       61 tctgatattg ttgcaggtaa taaaaattaa ctcttactgc tggatctatc ccataataaa
      121 ctaacaagtt tacacaatgt cttatttccc aacttgaata cgttacttat taacaaaaac
      181 ctgctttctg aaattaaaat attctatagc aacttctgca atgttcagac attaaacgct
      241 gctaacaacc agttgaaaaa tataaatctt gatttcctga cttgtcttcc atctattaaa
      301 agtttaagac tggacaacaa taaaataacc catattgata ctaataatac atccgatatt
      361 ggaactttat ttcccataat aaaacagagc aaaaacttaa attttttaaa tgtttctggg
      421 aagaacaatt gccctactat gcagctcatg ttatttaatt tgttttcccc aacacttaag
      481 cttaatactg gcctggcaat tctttcgcct ggtgcatttg aagttcactc tgacggaata
      541 gatgtggata acgaattgtt tcactatcct attaaaaata gcatataccc catataatat
      601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
      661 cttagatgaa ataaacaata cttactgtaa taacgattat tacaatcagg caataagaga
      721 ggaaccgata gaccttctgg acagatcgtt ttcctccagt tcatggcctt tttagtgaat
      781 aaatcatcac taattctata agtgtctggt cttgttgata atatagttaa tatgtaaccc
      841 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta accccgtttg
      901 aaggttagct tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc
      961 ccccgcggtt acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta
     1021 attctaataa ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg
     1081 tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
     1141 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
     1201 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
     1261 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
     1321 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
     1381 ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg
     1441 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
     1501 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
     1561 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
     1621 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
     1681 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
     1741 ggatttgggt accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata
     1801 tcgccagttc acgcgcctca attgacgacc agctccctgt cttacaacgc ctgcatctct
     1861 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
     1921 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
     1981 gcccatttat cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aaattcggcg
     2041 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     2101 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     2161 tgtttctgac tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     2221 gccagataac catttatcgg gtgaagatga aaggcggtag tgatgagcaa gctcagaaag
     2281 aagccatcac cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg
     2341 gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     2401 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     2461 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc
     2521 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     2581 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     2641 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     2701 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     2761 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     2821 agcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctctaca
     2881 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgtgaag
     2941 ctctcaagca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     3001 agtgcttaag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     3061 aacttaacca actgattgcc gccgctaacg tctggaccat caaaatctat ggtccggatc
     3121 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt gtcatacgct gccggaacgc
     3181 gttatctgtc gctgttaggc ggcacctgtt taagcttcta cgactggtat tgcgacctgc
     3241 cgcccgcctc gccgatgacc tggggcgaac aaaccgacgt gccggaatct gccgactggt
     3301 ataactccag ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     3361 cccacttctt taccgaagta cgttacaaag gcaccaaaac tatcgccatt acccctgact
     3421 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     3481 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     3541 actactttat caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac
     3601 ctcgcgacga tggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg
     3661 gactaggcga aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat
     3721 tggtagtgcc gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     3781 aatccattgc cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     3841 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     3901 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     3961 gcaacacctg tccggtggtc agcgtttatg atttggtgct ggcgaattac ggcctcgatc
     4021 gcgggctgga agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag
     4081 cctggggtga gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat
     4141 ttgccgatac cgcccataaa acgcatggac gctcaatgat tatcctcggc gctggcgtca
     4201 accactggta tcacatggat atgaactacc gtgggatgat caatatgctg atcttctgcg
     4261 gttgtgtcgg gcaaagcggc ggcggctggg cgcactatgt cggtcaggaa aaactgcgcc
     4321 cacaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgccca ccgcgccaga
     4381 tgaacagtac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     4441 ctcaggagtt actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt
     4501 tcaacgttcg cgccgaacgc atgggctggc taccttctgc gccgcagctg gggcgtaacc
     4561 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     4621 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     4681 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     4741 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     4801 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     4861 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4921 ttctgcccac cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt
     4981 ttattcatcc gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa
     5041 tctacaaagg tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa
     5101 ccgacgtggt attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg
     5161 aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata
     5221 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     5281 tgatggacaa acttggcaat ggtggtaaag ggatttcgtg gaatacccag gatgaaatcg
     5341 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     5401 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     5461 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     5521 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     5581 aaattatctc cagccccaca tggtccggtc tggaaagcga tcacgtctcc tataatgcgg
     5641 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     5701 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     5761 tcgacacccg tagcgtcagt gagatgcgcc agatcccgcc aaacggcttc ccggaaaaag
     5821 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     5881 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc
     5941 gagaactgac cattgtcgat aacgactggg tggaagtctt taacgccaac ggcgcgctga
     6001 cggctcgtgc ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgccc
     6061 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     6121 actcggtcac ccgtgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     6181 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     6241 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     6301 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgacaaat gtatcggttg
     6361 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     6421 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     6481 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg
     6541 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     6601 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     6661 tatccctact gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg
     6721 ggggccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     6781 cttcgaggcc atgcaaaagg agatgtacgg gcagtttaaa aacaccttca tgatgtacct
     6841 gccgcgcctg tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc
     6901 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
     6961 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     7021 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
     7081 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
     7141 tgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtgtt
     7201 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     7261 cgtgattgaa gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     7321 gctaccgctg caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc
     7381 gccgattcag tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc
     7441 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac
     7501 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     7561 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     7621 ggtcgaagaa atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc
     7681 gaccagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     7741 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     7801 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     7861 aaagtgatcg gcctgttgat ggagtatccg gatgagctgt tgtgggaatg caaggaggac
     7921 gcgctggcgt tgatccgccg cgacgcgccg atgcttacgg atttcaccca caacctgctt
     7981 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc
     8041 acgtcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     8101 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     8161 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     8221 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag
     8281 gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg cgggaagtat actttcaagt
     8341 gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat
     8401 gcagtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     8461 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca gtatgtcgac
     8521 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     8581 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt
     8641 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga
     8701 atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc
     8761 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactcatg gcgatggtgc
     8821 tcggcggtat ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcagtc
     8881 tgacaaatca gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt
     8941 tgctgatcca gtgtctgttg gggctaagta ccataccgtt ttcagcacag tatcctgatg
     9001 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     9061 cgtcagagat gctcaacggc gtagcgttcg tcttccgcct gcatctggtg ttgggaatga
     9121 ccatcttcct gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg ccgtttgagt
     9181 actttactcg tcgatatcaa attgtgcgtt cgcggcgata atttcaccct ggccggatag
     9241 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa
     9301 tgaaaccgca ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg
     9361 ccggtgttgt tttccccgcc gataatctta gcgaagcaca aatgcagctt atcgcccgcg
     9421 agttaggcca ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc
     9481 gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact
     9541 atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag
     9601 gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag
     9661 gtacgccggg ctttgaactg ccgctggagg gtgaagtacg cgcggcgatt atcaacgcat
     9721 tgcatcttac tgaagacgat attctgccgg gtttgccgat ccaggtcgcg acgaccgggc
     9781 attctaaagt gatgatccca ttgaagccgg aagtggatat cgacgctctt tcgcctgacc
     9841 ttaatgcgct gaccgctatc agtaaacaga ttggctgcaa tggtttcttc ccgttccaga
     9901 tccgtccagg caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg
     9961 aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg
    10021 tattgcccca cgatggcaac gtgttgtgtg ttaaaggcca tcaggggcgc gcattggggc
    10081 gtgacggcat gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca
    10141 tttcaggcac agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc tgaaatcgtt
    10201 gcctgatgcg cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt
    10261 tacgccgcat ccggcaacct gacataaatt attttcccgc ctccggatgc gtatcaaacg
    10321 ccgccatcac cagcgctaac tcatccacag taaagccatg tttcgcatca tccacgccca
    10381 gaccaaactg ttcctgcatc acggcatata acgacgccac atccggtaga tttcgctgct
    10441 ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagtgtc agcttgccgc
    10501 cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg
    10561 ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga tcgaagcggt
    10621 acatcggctg ccaatgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca
    10681 acaaccgata ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg
    10741 cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcccct tccagttcca
    10801 ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc
    10861 ctaacaagct gcgaacgtta aaccccagct cgcgtaacac ccgctcaaac acgccattct
    10921 gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat
    10981 caagctgtat ttccctcggc agcaaaacgt caaggttttc aaacggaatg gtgcaattgt
    11041 gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa
    11101 tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc
    11161 aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca
    11221 cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa
    11281 aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg
    11341 ccagccgtct gttaaatcat ggcccaactg tcttgatcac cagtttcgat gagcaatccc
    11401 agcggcgtaa cattatggct gcagcctggt caatgccggt ggagtttgaa cctccacgcg
    11461 tggcaattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaagt
    11521 ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcggtg ggaagtgtgt
    11581 cggtgcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtgc
    11641 ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg
    11701 cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca gcagcggcag
    11761 acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc
    11821 atcatttagg tgctggcacg tttgttacta gcggcaagcg tgttacggca ggttaagcat
    11881 tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggctcgat ttcggcatcc
    11941 cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct
    12001 ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttgt
    12061 tgttcgtcca gttcacgcgg aaagcattta atgtcgatgt gcggcatgat tacgctcctt
    12121 ttgaaagatg tcagtaaaat taacacacat tgtgaatgtc atgtgtccgg taaagcgttt
    12181 atacgacaat aatgcccagg gcaagattac gaaagcccgc tccccgtaag gactgacgcc
    12241 aggtagtttc tgcccatggc tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc
    12301 ttatcattcg tcaaaatatt aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca
    12361 tttcctcttc ttattttgca gtcgtcttca ttcattacca cgtccagacg cca
//
LOCUS       DAZRVY010000117        12409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZRVY010000117 DAZRVY010000000
VERSION     DAZRVY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.117"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHXOX_003302"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHXOX_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR7006692.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHXOX_003303"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHXOX_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR7006693.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHXOX_003304"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHXOX_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR7006694.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHXOX_003305"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHXOX_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR7006695.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHXOX_003306"
     CDS             7014..7292
                     /locus_tag="ACHXOX_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006696.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHXOX_003307"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHXOX_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR7006697.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHXOX_003308"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHXOX_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR7006698.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHXOX_003309"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHXOX_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR7006699.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHXOX_003310"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHXOX_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR7006700.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="ACHXOX_003311"
     CDS             10284..10931
                     /locus_tag="ACHXOX_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR7006701.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="ACHXOX_003312"
     CDS             complement(10973..11503)
                     /locus_tag="ACHXOX_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR7006702.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHXOX_003313"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHXOX_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR7006703.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2754 c         2808 g         3417 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAZRVY010000118        12080 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZRVY010000118 DAZRVY010000000
VERSION     DAZRVY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.118"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXOX_003314"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXOX_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR7006704.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXOX_003315"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXOX_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR7006705.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXOX_003316"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXOX_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR7006706.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHXOX_003317"
     CDS             complement(3668..3898)
                     /locus_tag="ACHXOX_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR7006707.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHXOX_003318"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHXOX_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHXOX_003319"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHXOX_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR7006708.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHXOX_003320"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHXOX_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHXOX_003321"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHXOX_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR7006709.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHXOX_003322"
     CDS             7629..8642
                     /locus_tag="ACHXOX_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7006710.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHXOX_003323"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHXOX_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHXOX_003324"
     CDS             9533..10327
                     /locus_tag="ACHXOX_003324"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7006711.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHXOX_003325"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHXOX_003325"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR7006712.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="ACHXOX_003326"
     CDS             11983..>12080
                     /locus_tag="ACHXOX_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR7006713.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2947 a         2913 c         3223 g         2997 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacacaaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaatc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAZRVY010000119        11881 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZRVY010000119 DAZRVY010000000
VERSION     DAZRVY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.119"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            112..678
                     /locus_tag="ACHXOX_003327"
     CDS             112..678
                     /locus_tag="ACHXOX_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7006714.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            834..2866
                     /locus_tag="ACHXOX_003328"
                     /pseudo
     CDS             834..2866
                     /locus_tag="ACHXOX_003328"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2843..3187
                     /locus_tag="ACHXOX_003329"
     CDS             2843..3187
                     /locus_tag="ACHXOX_003329"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR7006715.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3202..3933
                     /locus_tag="ACHXOX_003330"
     CDS             3202..3933
                     /locus_tag="ACHXOX_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7006716.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3930..4991
                     /locus_tag="ACHXOX_003331"
     CDS             3930..4991
                     /locus_tag="ACHXOX_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7006717.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5143..6189
                     /locus_tag="ACHXOX_003332"
     CDS             5143..6189
                     /locus_tag="ACHXOX_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR7006718.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6186..6977)
                     /gene="nei"
                     /locus_tag="ACHXOX_003333"
     CDS             complement(6186..6977)
                     /gene="nei"
                     /locus_tag="ACHXOX_003333"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR7006719.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7013..7747)
                     /gene="pxpA"
                     /locus_tag="ACHXOX_003334"
     CDS             complement(7013..7747)
                     /gene="pxpA"
                     /locus_tag="ACHXOX_003334"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR7006720.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7737..8669)
                     /gene="pxpC"
                     /locus_tag="ACHXOX_003335"
     CDS             complement(7737..8669)
                     /gene="pxpC"
                     /locus_tag="ACHXOX_003335"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR7006721.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8663..9319)
                     /gene="pxpB"
                     /locus_tag="ACHXOX_003336"
     CDS             complement(8663..9319)
                     /gene="pxpB"
                     /locus_tag="ACHXOX_003336"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR7006722.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9342..10085)
                     /gene="ybgI"
                     /locus_tag="ACHXOX_003337"
     CDS             complement(9342..10085)
                     /gene="ybgI"
                     /locus_tag="ACHXOX_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR7006723.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10356..11837
                     /gene="dtpD"
                     /locus_tag="ACHXOX_003338"
     CDS             10356..11837
                     /gene="dtpD"
                     /locus_tag="ACHXOX_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR7006724.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2856 a         3167 c         2900 g         2958 t
ORIGIN      
        1 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
       61 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
      121 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
      181 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
      241 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
      301 aatcagtcac atcacagcga tgccgttgct gtcgacctgc gcttagtcaa ctgtgatctg
      361 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
      421 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
      481 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
      541 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
      601 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
      661 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
      721 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
      781 cgatgccttg cgcattggcg gtcgaattca accttaatgt tctcgataaa tcgatgcgcg
      841 accgcattga tatttcctta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
      901 ttagcgttgc ggtaaataat aaccaaatca gtaacgggca aaagattaac tggcacaaaa
      961 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
     1021 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
     1081 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
     1141 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
     1201 gtatcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggac ggcagcagca
     1261 gcactaacct gaacgcctac ggtaccaccg gaattaacgc cggggcatgg cgcttacgta
     1321 gtgattatca gttgaatcag actgatagcg atgataacca tgaacagtca ggcggaatat
     1381 cgcgcaccta tctttttcgt ccattaccgc aattaggctc taaattaacc ctcggcgaaa
     1441 cggattttag ttccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
     1501 acgagcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
     1561 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
     1621 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
     1681 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcggcagca tcgacgccct
     1741 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
     1801 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
     1861 caaacacctc gctgtacggc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
     1921 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
     1981 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
     2041 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
     2101 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
     2161 aaaaacagac gatcagctta ttgtgggcca accgattacc ccactaaacc tcaatcttta
     2221 cgccaacctg ctacatcaaa cctggtggaa tgcagacgcc tccacgaccg ccaacatcac
     2281 ggcaggtttt aatgttgata ttggtgactg gagagatatc tctatttcga cgtcattcaa
     2341 tacaacccat tacgaagata aagatcacga caaccagatt tacctgtcga tttcgctccc
     2401 cttcggtaac ggtggtcggg ttggctatga catgcaaaac agtagccaca gcaccacaca
     2461 ccgcatgtcg tggaacgata cgctggatga acgtaatagc tggggcatgt ctgccggact
     2521 gcaatccgac cgtcctgaca atggagccca ggtgagcggt aactatcagc acctgagttc
     2581 agcgggtgag tgggatattt ctggtaccta tgccgccaat gattacagtt ccgtcagcag
     2641 cagctggagc ggttctttca ccgcaaccca atatggtgca gcgtttcatc gccgcagctc
     2701 caccaatgaa ccacgcctga tggtcagcac cgatggcgtg gcagatattc cggttcaggg
     2761 caatctcgac tacaccaacc attttggcat tgcggtggtg ccgttgattt ccagttacca
     2821 gccttccacc gtggcggtga acatgaatga cttacccgac ggcgtaacag ttgcagaaaa
     2881 cgttatcaaa gaaacgtgga ttgaaggcgc gataggttac aaatcactgg cttcccgttc
     2941 cggtaaagac gttaacgtca tcattcgcaa cgccagcggt cagtttcctc ccctcggagc
     3001 ggatatccgc caggatgaca gcggcattag cgtggggatg gttggcgagg aaggacatgc
     3061 ctggttaagc ggagtcgctg aaaatcaaaa gtttaccgtg gtctggggtg atagccagca
     3121 ttgctcgctc catcttcctg aacatatgga agacaccgca aatcgcctga ttttaccttg
     3181 tcattaatta aggaaatgaa aatgacattt atgaaaggac tacccctttt gttgttggtt
     3241 gccagtctgt gcagccacgc tgcactacaa cccgatcgca ctcgtattgt gtttaacgct
     3301 aatgataaag ctaccagtct gcgggtggat aatcgtagcg ataaactccc ctacctcgct
     3361 tattcctggc tcgaaaatga aaaaggggag aaaagcgatg atcttctggt cgccctgccg
     3421 ccgatacaac gtctggaacc gaaagcaaca acgcaggtac ggatagtaaa gcaagcctcg
     3481 accacaaaat taccgggcga tcgcgaaacg ctatttttct acaatatgcg tgaaattcct
     3541 cccgccccgg aaaaaaacag cgaccatgcc gttctgcaag tcgccattca gagtcgtatc
     3601 aaggtgttct ggcgaccggc tgcattacgc aagaaagcag gagaaaaggt cgaactgcag
     3661 ttacaggtca gccagcaggg caaccaactg acactgaaaa atcctaccgc gtattatctg
     3721 accattgcct acctgggacg aaatgaaaaa ggcgttctcc ccggcttcaa aaccgtgatg
     3781 gttgcgcctt ttagtacggt caacacaaac accggaaatt atagcggcag ccagttttat
     3841 ctcggttata tggatgatta cggcgcgtta cgcatgacga cgctcaactg cagcggacaa
     3901 tgccatttac aagccgtgga ggcgaagaaa tgagtgctgg caagggagtg ttgctcgtca
     3961 tctgtctgtt atttctgccg ttgaagtctg cgcttgccct gaactgctac tttggttcat
     4021 ccggtggctc ggtagaaaaa tcagaagcga ttcaaccttt tgctgtacca ggcaatgcca
     4081 aactcggcga taagatttgg gaatctgacg atattaaaat tcccgtctat tgtgacaaca
     4141 ataccaacgg caattttgaa agtgagcatg tttatgcctg ggtaaccccc tacccggggg
     4201 tgcaggatcg ctattatcaa ctcggcgtaa cttacaatgg cgttgattat gacgccaacc
     4261 agggaaaaag ccgcatcgac actaaccagt gtattgacag taagaacatt gacatttata
     4321 cccctgagca gattatcgcg atgggatggc agaacaaaat ttgctcgggt gatcccgcta
     4381 atattcacat gtcgcgtacc tttctcgctc gcatgcggtt atacgtcaaa atacgagaaa
     4441 tgccgccgca tgattatcaa agtacgctta gcgactatat cgtcgtgcaa tttgacggtg
     4501 ccggtagcgt taacgaagac cctactgccc aaaacctgaa atatcatatt actggtctgg
     4561 aaaacattcg cgtgctggat tgcagcgtca atttttccat ttcaccggaa acacaggtga
     4621 ttgattttgg taaatttaat ttgctggata ttcgtcgcca cacaatgtcg aaaacattca
     4681 gtattaagac gacaaaatca caaaatgacc aatgcaccga cggatttaaa gtcagttcct
     4741 ccttttatac cgaagaaacg ttggttgaag aagataaagc attgttgatt ggtaatggtc
     4801 tgaagctacg tttgctggat gaaaacgcct cgccttatac cttcaataaa tatgctgaat
     4861 acgccgattt caccagcgac atgttggtct acgaaaaaac ctatacagct gaactttcgt
     4921 ccattgcagg tacccccatc gaggctgggc ccttcgatac agtggtgctt tttaaaatta
     4981 actataactg atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat
     5041 tgctgatttt ccccttttcc ttgattgctg ttattttttc ctggcccggc tttctttatg
     5101 ctgtatcgat acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg
     5161 ggaatgttat gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg
     5221 gcggcgctat tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact
     5281 ctgcaacttc cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg
     5341 caaaacctca ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct
     5401 attttactgg tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc
     5461 tcactgcctg gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc
     5521 gctatggcgc aagattacgg cgcagatatt cgcctggttg catttatgca atatctgcga
     5581 gtgctgttcg tcgcgggggc cgctgttctg gtcacccgca tgatgctggg tgataacgct
     5641 gaagcggtta accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg
     5701 attttgctcg cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg
     5761 atgctcatcc cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa
     5821 ctaccggaat ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt
     5881 ttcgataaac aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt
     5941 tttgctctgc tggctatttg tgcgggtatg gcgtgggggc tgacccggtt tatgcacatt
     6001 gattttatga ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc
     6061 gccgcaggga gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc
     6121 agtattttgc tgacggggcc agccattgcg cggtttattt caacctatgc gccgaagcgg
     6181 tcggcctagt gctggcagcc agggcaccag taaaacgggc gagatgacag cgtggttttc
     6241 tcaatgatgc tgccacagcg ttcgcacggt tcgccatcac gatgaaaaac cttaaagcga
     6301 aacagcgccc catgatgctt attctcatcc acctgccccc tcgtagcgta ggaaaatcga
     6361 ggaatatcca gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgct
     6421 ttatgatttc cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc
     6481 ccagccagaa acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggt
     6541 gacaataacc gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca
     6601 acgcgttgta aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag
     6661 gcgctataaa gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg
     6721 gtctgcggct cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg
     6781 tagagcgtta agtcgttgga aaaatgagtt aacaacgcct taccacgcgt ttccacatgg
     6841 gtaacgtgtt gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag
     6901 acatcagtta gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc
     6961 tccgggcctt caggcatggt gatatccttt tttcattctt tattaatcgg ggttatgctg
     7021 cgacaacaat ctccttttcg gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt
     7081 gctcgccgtc gccatgcagg cagactgttt gcgccgtgac cgttgcccat tcgccggtga
     7141 tacttttgac tctgccgtgt tgcaccatct ctagcgtttg cgccaacgcc tgttcttcgt
     7201 tttcaatcaa cgcgcctcgc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc
     7261 gatcggcaaa cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct
     7321 cgcttcctgc cagcccaacg agaatcaatg ccggatcgca agcgtatacc gctctggcga
     7381 tggcgtctgc cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga
     7441 catgacgcat tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca
     7501 gcgtctgggc gtaaacggtt tctggcggca gctgcatggc gctgcgacca aaattttccc
     7561 tgtcgggaaa actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc
     7621 aagcctgcat gatttgcgca tcgcctgcat gaaatccaca ggcaatattg gcagaggaaa
     7681 ccagcgttaa tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa
     7741 ttttcattgt gcagccgcca cactaattgt tcgaaataac gttgctgatc ttgccgcgct
     7801 ttcagtgcct cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc
     7861 gccagatggt acatatcagc ctcaatgata caggcaatac gcgggtaacc atcggtggtc
     7921 tgtgcgtcgt tcatcaacac aatcggctgc ccgttatgtg gcacctgcac cacgcccggt
     7981 aacaaaccgt gagataacag ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag
     8041 cgatagccca tgcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc
     8101 tgcgaggcgc gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc
     8161 cacagcagct gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg
     8221 agtcggtcac catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg
     8281 gtgctgcatg accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg
     8341 cgcatcccgt gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc
     8401 cagccggtcc agacggcatt atcatccagc cgcgcttcgc aaccggcacc cgtcagagca
     8461 aaccacccat cagtttcgaa ctcaaccgtt aactgaccga gcgtgatctc cagcgcgggg
     8521 gcattggcgt cattacccac cagcaggtta gcaatgcgta acgcaggcat atccagtgcg
     8581 ccgcagtggc tgattcccga ctggcgaaaa ccgtgacgac cgccatcctg cacagtggta
     8641 tacatgcccg cacgaataat cttcagcata ctccctcctt ctgcggtaca aagcgcacgc
     8701 tgtctcccgg acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg
     8761 tatgaccaat caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc
     8821 cgccgatccc gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt
     8881 gtagttgttc cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga
     8941 ccacgtattc cacggaggag tgcaattcaa caacctgttt ttcgctaaac ccgcaatgcg
     9001 ccgcgaccac cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa
     9061 agcgagactc aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg
     9121 catccagcgc cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa
     9181 tggcttcaac cacattcggc atatccacca gacgctgcgc cagtcgccag atccgtttct
     9241 ggctagccag cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat
     9301 aacaacgcgc tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata
     9361 tcaataaagg tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata
     9421 ccaccacgtt cagtggcatg gtgacctgca gcataaaaat gcaacccctg ctcgcgggct
     9481 gaatgaatgg tctgttcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg
     9541 gcgctatcga taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca
     9601 ggtccggtat cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct
     9661 agttccagtc ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg
     9721 cccatgaccg tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt
     9781 acgtcaagcg gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa
     9841 cggttacgct tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc
     9901 acaatgaccg catccgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg
     9961 acaccggtaa caattttttg caccgtctct ttgccttcta cctgcaaacc attcggcgcg
    10021 taatcactaa tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt
    10081 ttcatcttcg cgccttccgt attctgatag ctacaccgtt aacatagcgt tacctcgcct
    10141 gttcgtcgat agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa
    10201 attgtttata tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca
    10261 taaataatca gatctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac
    10321 tattacgtga cgttgcagtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc
    10381 gcactattta ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc
    10441 gtgccctgct gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg
    10501 agttatttag cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg
    10561 cggataaagt tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg
    10621 gtcatgtggt gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga
    10681 ttatcgtttg tggctatggt ctgtttaaat ccaatgtcag ttgcctgctc ggcgagctgt
    10741 atgagccaac cgatccgcgt cgtgatggcg gtttctcgct gatgtatgca gcgggtaacg
    10801 tggggtctat tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga
    10861 tgggctttgg cctggcggcg gtgggtatga tcgcgggtct ggtcattttc ttatgtggca
    10921 atcgtcattt cacacatacc cgcggcgtta ataaaaaagt actgcgtgca acaaactttc
    10981 tcctgcctaa ctggggatgg ctgctggttc tgctggtggc aacgcctgcg ctgattaccg
    11041 tactgttctg gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg
    11101 gtgtactggc aaaaatttat cgcaaagcag aaaaccagaa acagcgtaag gagctggggc
    11161 tgattgtgac gctcaccttc ttcagtatgt tgttctgggc cttcgcacaa cagggcggca
    11221 gctcaattag cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc
    11281 cgactgctat gttccagtcg attaatgcct tcgcagttat gctatgcggt gtgttcctgg
    11341 cgtgggtggt taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg
    11401 ctcttggcct tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt
    11461 ccgcaatgta tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct
    11521 ttgcggaact gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg
    11581 gcgtgaccgg cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc
    11641 tggcgggcgt gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca
    11701 actactccat caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt
    11761 gtgtaggact ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc
    11821 tggcgctgga gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat
    11881 c
//
LOCUS       DAZRVY010000120        11810 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZRVY010000120 DAZRVY010000000
VERSION     DAZRVY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.120"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHXOX_003339"
     CDS             <1..488
                     /locus_tag="ACHXOX_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7006725.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="ACHXOX_003340"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="ACHXOX_003340"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR7006726.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHXOX_003341"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHXOX_003341"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR7006727.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHXOX_003342"
     CDS             complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHXOX_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR7006728.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHXOX_003343"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHXOX_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR7006729.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHXOX_003344"
     CDS             complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHXOX_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR7006730.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHXOX_003345"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHXOX_003345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR7006731.1"
                     /translation="MIAHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHXOX_003346"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHXOX_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR7006732.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="ACHXOX_003347"
     CDS             complement(6839..7747)
                     /locus_tag="ACHXOX_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7006733.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="ACHXOX_003348"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="ACHXOX_003348"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR7006734.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="ACHXOX_003349"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="ACHXOX_003349"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR7006735.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11810
                     /locus_tag="ACHXOX_003350"
     CDS             10715..>11810
                     /locus_tag="ACHXOX_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR7006736.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
BASE COUNT         3004 a         3001 c         2930 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattgc gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg
//
LOCUS       DAZRVY010000121        11779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZRVY010000121 DAZRVY010000000
VERSION     DAZRVY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.121"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHXOX_003351"
     CDS             complement(<1..611)
                     /locus_tag="ACHXOX_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR7006737.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHXOX_003352"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHXOX_003352"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR7006738.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHXOX_003353"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHXOX_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR7006739.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHXOX_003354"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHXOX_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR7006740.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHXOX_003355"
     CDS             complement(5085..6716)
                     /locus_tag="ACHXOX_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR7006741.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHXOX_003356"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHXOX_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR7006742.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHXOX_003357"
     CDS             7139..7411
                     /locus_tag="ACHXOX_003357"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7006743.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHXOX_003358"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHXOX_003358"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR7006744.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHXOX_003359"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHXOX_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR7006745.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHXOX_003360"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHXOX_003360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR7006746.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHXOX_003361"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHXOX_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR7006747.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHXOX_003362"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHXOX_003362"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR7006748.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caagtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZRVY010000122        11698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZRVY010000122 DAZRVY010000000
VERSION     DAZRVY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.122"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHXOX_003363"
     CDS             197..301
                     /locus_tag="ACHXOX_003363"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR7006749.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHXOX_003364"
     CDS             complement(350..1603)
                     /locus_tag="ACHXOX_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7006750.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHXOX_003365"
     CDS             1710..2603
                     /locus_tag="ACHXOX_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7006751.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHXOX_003366"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHXOX_003366"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR7006752.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHXOX_003367"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHXOX_003367"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR7006753.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHXOX_003368"
                     /pseudo
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHXOX_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHXOX_003369"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHXOX_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR7006754.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHXOX_003370"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHXOX_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR7006755.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHXOX_003371"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHXOX_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR7006756.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHXOX_003372"
     CDS             complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHXOX_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR7006757.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITNIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11698)
                     /locus_tag="ACHXOX_003373"
     CDS             complement(10807..>11698)
                     /locus_tag="ACHXOX_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR7006758.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2959 a         2963 c         3020 g         2756 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgctagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgttg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg caggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggca
//
LOCUS       DAZRVY010000123        11469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZRVY010000123 DAZRVY010000000
VERSION     DAZRVY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.123"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..611
                     /locus_tag="ACHXOX_003374"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHXOX_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /gene="prfH"
                     /locus_tag="ACHXOX_003375"
     CDS             608..1222
                     /gene="prfH"
                     /locus_tag="ACHXOX_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR7006759.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHXOX_003376"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="ACHXOX_003376"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR7006760.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="ACHXOX_003377"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="ACHXOX_003377"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR7006761.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="ACHXOX_003378"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="ACHXOX_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR7006762.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="ACHXOX_003379"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="ACHXOX_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR7006763.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHXOX_003380"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="ACHXOX_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR7006764.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="ACHXOX_003381"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="ACHXOX_003381"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR7006765.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="ACHXOX_003382"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="ACHXOX_003382"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR7006766.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="ACHXOX_003383"
     tRNA            9115..9190
                     /locus_tag="ACHXOX_003383"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            9796..9954
                     /locus_tag="ACHXOX_003384"
     CDS             9796..9954
                     /locus_tag="ACHXOX_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006767.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2972 a         2669 c         2892 g         2936 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc acagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcacaa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga ttaaaatata aacagataat tatgtatctt attgatttat
    10201 aaggttattt ttaagtaaag gtaaataaaa aataaatagc cataaatagc tataaataca
    10261 tcttaatacc tatatccctt cccttatggc tagattgcga gaggtgctta acgcgatttt
    10321 cagcgccccc cgatctggaa aatccagaat ccccaaatat cagcaaggaa ttttcttgcg
    10381 taatcaacat cgtttttacc tattaaatca ttacacagaa agcctttctg taatgtctct
    10441 cactaaactt tcactaaaca cgagaatgat cggttttatg tcggatcaga tgctagaaac
    10501 agcaccacgt ctcacaaggg ctgtaagcga tgaaaccagt gtttatgcgg gtgcaggtca
    10561 aaatataggc caaaatccac ttaacataat aaatgttata tgtgctaagg aacacatata
    10621 acgccttgaa ttgatgtatc aggggaaaag tgatggattt tttacgtagg agcacgaaag
    10681 ggcttctatt gacttttgta tgctatgaaa tataattaaa gttttttgtt tttattgtgt
    10741 gatgtggtgg gttacacagg ggaatagatt aaatggtgtt ctgtttatac aggtttattt
    10801 gttagatcaa caatttggca tttctaaaca gatgtttgaa cgttgccaaa tgtcttgaca
    10861 acacaagaaa agggcttgct atgccaggca ttacacgaaa taaggctata aaaacaaaat
    10921 aaattttaaa atttgcacaa acgagaaact cgttcagaaa caagcagaac aatttttagc
    10981 caacaatttc tgatggtgct ctgtaagccg cattacacaa ggctttgcga aagtgatgta
    11041 aaagtgaaga aagtaagaat tgaactttta agctactttt cattggattt tttagtaggg
    11101 aattacacag ggaatagaga gcattattct atgatttaca cagggaatat agggggatta
    11161 tggcacacac atagaaagac attacacaga gtgctatgtg acaaatggat gtaacgagag
    11221 gttacattca tttatctgaa atgctctctg taagaaagtt ggtagtaacg atatgttaca
    11281 tagactaatt attgaatgta acgatattac acatcggtag taacgatacg ttacatcgga
    11341 agatgattgg atgtaacggt agctcacagt gaactagggg taggtcatag tgaactaccc
    11401 ccacagaatt ctgttacccc tatcaagtct gataccccta tcaaaactga tacccgataa
    11461 gtataaata
//
LOCUS       DAZRVY010000124        11461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZRVY010000124 DAZRVY010000000
VERSION     DAZRVY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.124"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHXOX_003385"
     CDS             460..1386
                     /locus_tag="ACHXOX_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR7006768.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHXOX_003386"
     CDS             complement(1336..2529)
                     /locus_tag="ACHXOX_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7006769.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHXOX_003387"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHXOX_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR7006770.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHXOX_003388"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHXOX_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR7006771.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHXOX_003389"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHXOX_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR7006772.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHXOX_003390"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHXOX_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR7006773.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHXOX_003391"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHXOX_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR7006774.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHXOX_003392"
     CDS             complement(11165..11308)
                     /locus_tag="ACHXOX_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006775.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2899 a         2887 c         2939 g         2736 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 c
//
LOCUS       DAZRVY010000125        11428 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZRVY010000125 DAZRVY010000000
VERSION     DAZRVY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.125"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXOX_003393"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXOX_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR7006776.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXOX_003394"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXOX_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR7006777.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXOX_003395"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXOX_003395"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR7006778.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXOX_003396"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXOX_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR7006779.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHXOX_003397"
     CDS             complement(3928..5214)
                     /locus_tag="ACHXOX_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR7006780.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHXOX_003398"
     CDS             complement(5235..6026)
                     /locus_tag="ACHXOX_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR7006781.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXOX_003399"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXOX_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR7006782.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXOX_003400"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXOX_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR7006783.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXOX_003401"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXOX_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR7006784.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXOX_003402"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXOX_003402"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR7006785.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXOX_003403"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXOX_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR7006786.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHXOX_003404"
     CDS             complement(9882..10364)
                     /locus_tag="ACHXOX_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR7006787.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHXOX_003405"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHXOX_003405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR7006788.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZRVY010000126        11363 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZRVY010000126 DAZRVY010000000
VERSION     DAZRVY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.126"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            258..758
                     /gene="tssB"
                     /locus_tag="ACHXOX_003406"
     CDS             258..758
                     /gene="tssB"
                     /locus_tag="ACHXOX_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR7006789.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            793..1017
                     /locus_tag="ACHXOX_003407"
     CDS             793..1017
                     /locus_tag="ACHXOX_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006790.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1068..2543
                     /gene="tssC"
                     /locus_tag="ACHXOX_003408"
                     /pseudo
     CDS             1068..2543
                     /gene="tssC"
                     /locus_tag="ACHXOX_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2550..2963
                     /gene="tssE"
                     /locus_tag="ACHXOX_003409"
     CDS             2550..2963
                     /gene="tssE"
                     /locus_tag="ACHXOX_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR7006791.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2967..4817
                     /gene="tssF"
                     /locus_tag="ACHXOX_003410"
     CDS             2967..4817
                     /gene="tssF"
                     /locus_tag="ACHXOX_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR7006792.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4781..5863
                     /gene="tssG"
                     /locus_tag="ACHXOX_003411"
     CDS             4781..5863
                     /gene="tssG"
                     /locus_tag="ACHXOX_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR7006793.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5888..7168
                     /gene="tagH"
                     /locus_tag="ACHXOX_003412"
     CDS             5888..7168
                     /gene="tagH"
                     /locus_tag="ACHXOX_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR7006794.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7165..7689
                     /gene="tssJ"
                     /locus_tag="ACHXOX_003413"
     CDS             7165..7689
                     /gene="tssJ"
                     /locus_tag="ACHXOX_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR7006795.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7692..9023
                     /gene="tssK"
                     /locus_tag="ACHXOX_003414"
     CDS             7692..9023
                     /gene="tssK"
                     /locus_tag="ACHXOX_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR7006796.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9028..9789
                     /gene="icmH"
                     /locus_tag="ACHXOX_003415"
     CDS             9028..9789
                     /gene="icmH"
                     /locus_tag="ACHXOX_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR7006797.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9798..>11363
                     /locus_tag="ACHXOX_003416"
     CDS             9798..>11363
                     /locus_tag="ACHXOX_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7006798.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         2680 a         3080 c         3059 g         2544 t
ORIGIN      
        1 ctatatttaa attattgcca taatacacct gacgcgaatt gcacaggttc acacaatcta
       61 tttaacgctt gcagaatacc cctctttacg atcgataaaa agtccttttc ttatttattt
      121 tgaagcgtaa aatgcggggt gatgaaggag tggtgatttt attttcaccg ccattagggc
      181 ggcattgccg atattttttt gcggtacaaa tatgccgtgt tcatggcctg tacgagataa
      241 catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgccc cgcgagcgta
      301 ttaatatatc gtatgtccca aaaacagatg gccagacggc tgaagtggaa cttccgctta
      361 acatgctcgt tgtcggcgat accggaaata cgcaggaaac atccccactg gatgaacgtc
      421 aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca gccattggcc
      481 tcaattttac tgtccccgcc acgctgaagg gaagcacaac agacgatgaa ctgaatgtgg
      541 cgctgaatat caaatcactg gatgattttt caccagatag cgtggcccgg caggttccgg
      601 aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg ccgatgggta
      661 accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag tcccgcgagc
      721 agttgctcaa agaaatcggt ctggtgagca ataaataacc accactattt cattaaacag
      781 ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct ctctgttgca
      841 gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat caaccaggat
      901 ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt ggatgagatt
      961 atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact tcactgatgg
     1021 cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg tctttacagg
     1081 aagaggaact cgtttccagt catgccgggc agcctgagca ggcatcgtcg ctgcttgatc
     1141 aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt gccctccagg
     1201 gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa ccggtcaata
     1261 aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc cagatggatg
     1321 tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca ctgaaaacca
     1381 tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat gtcaccaagc
     1441 aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga ttttataagc
     1501 acgtttattc gtccggtttc ggacagtttg gcggtaaacc catcgctgcc gtgctgggcg
     1561 cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc agtgccgtgg
     1621 gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg gggctgaact
     1681 cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt ccggcctaca
     1741 ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt acggcacccc
     1801 gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac tttaactatt
     1861 acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc tggatgctgg
     1921 catgcaatat cgcagacagt tttgccaaat accgctgatg tccaaacatt atcggtccgc
     1981 aaagcggcgg cgcagtgaaa gatctgccga tgcatctgtt cgaaacgatg gggcagattc
     2041 aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa ctggctgaag
     2101 agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt ttctctgcaa
     2161 actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac tataagctgg
     2221 gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc aaggtgttgc
     2281 agcgtgaaca actggggtca tggaaagaaa ggagcgactt agaaagagag cttaatacct
     2341 ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc agacgtgcgc agccgcaaac
     2401 ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg cgaacccggc tggtaccagg
     2461 ttgcgctaag cgtgagacct cattttaaat ttatgggggc aaattttgag ctttccctgg
     2521 ttggccggtt agacagggag taacacacca tgataccgga tcgggaaaga accacaggtt
     2581 ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg gcagggaggc agtattcatt
     2641 cgctcaggca atccattcgc cagaacctac gaaacattct taatacccgg tccggtagtt
     2701 gccggggtgc gcctgagctg ggcattgatg aaccggaagg ggctgaaaac ttccgcgaat
     2761 ccatgagtcg ggccatcgag caatgtatag aacgctatga accccggatc tcacacgctg
     2821 aagtgcaggc agtggtttca tccgcctcca gcccgctgga tatgacgttt catattacag
     2881 cctgggtgac atttaacgaa acgcatgagg tgcttgaatt tgatatggca ccgaatggca
     2941 gccagcatta ccgggtggac tgacaaatgg aatttgaaga acgctatttc cgggaagaac
     3001 tcgattacct gcgccagctt agcaagctgc tggcaacgga aaaaccccat ctggcccgct
     3061 tcctggccga aaaagatgca gatccggata ttgaacgcct gctggaaggg gtggcttttc
     3121 ttaccggcaa tctccgccag aaaattgagg atgaattccc agaactgacg cacgggctta
     3181 ttaagatgct atggcctaat tacctgcgtc cggttccggc aatgaccctt attgaatata
     3241 cgccggatat ggataagtct tctgtaccgg tgttaatccc ccgtaatgag cagtttacaa
     3301 ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc tgatgctaaa aaggaggagc
     3361 cgcctccctg taccttcacc ctctgccggg atatctggct gctgcccgtt cgcctggagc
     3421 agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa catcaccttt tcggtcgcac
     3481 cgggaacgga cttccgcacg ctggatctga acaaacttcg cttctggctc ggcaatgacg
     3541 acaactatac ccgtgaccag ctttatttat ggttctgcga atacttgcag ggtgccgacc
     3601 tgactgtggg tgaacagcat attcgcctgc ctgagtttat gctaaaagct gtcggttttg
     3661 agccgcagga tgccatgctg ccctggccga aaaacgtcca cagcggctac cggatccttc
     3721 aggagtattt ctgttacccc gatgcgtttc tcttttttga tctttgtggt tgtccggctt
     3781 tgcctgacgg attgcaggcg gaattcttta ccctgcaact gcgtttttcg cgccctttgc
     3841 ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta ttgcgcacct gccattaatt
     3901 tatttatcca ccatgcagaa gccatcacgc tggacaaccg gcgggcagac tatccgctgg
     3961 ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt gtggtgagcc
     4021 aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg caggccgcgc
     4081 gccagtggcc agcctttgaa agtttcagcc atcagataga atacagccgg aagcgggaag
     4141 tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt gatcataccc
     4201 ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc agtaatgaag
     4261 tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt cgttccggcg
     4321 atatcaccgg cacaaccgga aaaaatgcgg ctgttgcttc atttcgcaac attacccgcc
     4381 cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggccccta ctctccgcca
     4441 tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc atcgccaact
     4501 ttgatcgcca cgcaatccat catccgcaga cggcacggct gtcacaacaa aagctggatg
     4561 ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt cccgtccggg
     4621 ggctggcttc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg gaaatgtatc
     4681 tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac tcattccaca
     4741 tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa agaatcgggc
     4801 agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga atgatccgct
     4861 ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg agctgttgca
     4921 ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgcgacg atccggcaca
     4981 ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg atatcagtgc
     5041 gctgaaacgg gatacgtcag gggcattcag gatgaccacc acctttatgg ggttacaggg
     5101 gagccagtcg cctctccccg gttattacct cgctcacctg gcctggaaag cggtacatga
     5161 acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc agtttgtctg
     5221 gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg tggatgcgtt
     5281 ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg ataagctggc
     5341 gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg gacgatcccc
     5401 ggaaattatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga cactgcaaaa
     5461 ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aatagtctgg ggagctattc
     5521 tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg ttctgggtac
     5581 acgagtgccc gatctcagcg gaaaattcca gctcagtatt accagcctga cccgaaaaca
     5641 gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgatgt tcgtgtcctt
     5701 catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg agcaggtggg
     5761 cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct tcctcggcac
     5821 accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga taatcacgga
     5881 gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta acggcagtga
     5941 gctggaaagc gggcgggcgg cgcgctgtct gttcacacag cagggaaatg tcggccatgg
     6001 ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc aggcttttac
     6061 cgttatcctg cacgatggca cattttgtct gcgcccgcag acggcacaac tgtggctgaa
     6121 tcaggcaaaa gtcacagcaa catcagacct aatacagttg cgccagggcg atgagatcca
     6181 gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc attacgatga
     6241 ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg ataccctgct
     6301 atcaacggag ggtgcgccac actatccggg aatgactcac cggcaccagc ttgcagacac
     6361 cgtggtaaac ggtttttctg ccgatccact ccaggctctt cagtccgaaa gcctgattac
     6421 cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc tgtccgatcc
     6481 ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt atgccagccc
     6541 cggcgatcgt aatgacgacg tctctgcggc agaaatggcg caacgccacg ttgcggtcac
     6601 ccccttactg cgcggtcttg gcggctcgct taccatgagc aattccgacg atgcggatga
     6661 ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc tcgatttgca
     6721 gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata acccgctgcg
     6781 cctgaacatg gattacgcca ccgcgctcga cgtaatgttt gccgaaggta aaagcccggt
     6841 acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc accacgaaga
     6901 agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg ctggatgctt tctcaccaca
     6961 aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc agccgctgga
     7021 tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct ccgatcgcca
     7081 gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc gggtgcttcg
     7141 tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg cgtgctgatg
     7201 tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gtgaggtgat caaaaacccg
     7261 gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac cctgctgacc
     7321 gaaccggaca ccaataccaa tgcggaaggt gaatcagccg ccgtggatgt gcaactggtg
     7381 tacctgactg acgactcaaa attgctggca gccgactatg accagatagc cagcacgccg
     7441 ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct gttgccggac
     7501 accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa cgcagtttat tggtgtcgtg
     7561 gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt ggaaggaatc
     7621 ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat gaaaaaagag
     7681 gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg atgctgatgc
     7741 gtccacacca tttccagcag cagcagcgtt acaacgacta cctggataac cagcgtttcc
     7801 gggccatgaa tgatttatcc tggggattta ccgaactcac cctcaacaat gaactgctgg
     7861 cgcagggtaa gatcatgatt gacagcgcgt caggcacact gcccgacggc accgtctttt
     7921 ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaaaatttt ccggacgaaa
     7981 gaagccgcaa tatctacctc gctctgccgg tcgccagtga tgtgagaaat gaaatcagcg
     8041 acggacggcg aatcgggcgt taccggctga attatgccga tgtccgggat ttgcattcag
     8101 aagaaggtga cgcgcgaaca ttgacgctgg gtcaactgac gccgcgcatt atgagcggtg
     8161 cagaagatat gagcgcctat attacgctcc cgctttgccg tatcagcgat cgccatgccg
     8221 atggctccct gacgttggat gacgatttta tcccctcctg ccagaatatt caggtcagta
     8281 agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg gcaagcgatc
     8341 tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg gaatttatga
     8401 tgttgcagtt acttaaccgt aaccagaccc ggtttaccca tcacgcccgt cgatcccagc
     8461 tccacccgga agatttctac cttgatcttg ccgggttgct gggtgaactg atgaccttta
     8521 ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga tcatcatgac ctgaccaaaa
     8581 tatttaaaac gctgttaccg gaagtcaaac gggcgctgca taccgtactg tcgccaagag
     8641 ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca ggccgatatc catgacacag
     8701 aactgctgca atctgccacc tttgtgctgg ccgtggcggc aaatatgccg gtcgatcaga
     8761 tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc cgcaatatgg
     8821 tcagtgtgca gatcccctgt attccattgc gtgcccttat ggtggctccc cgccagcttc
     8881 cttaccattc cgggttcagc tatttcgaac tcgacaagag tggccaggcc tggacagaaa
     8941 tggctgccgc cggggccgtt gcactgcatg tttccggcag tttcccggat ctgaacatgc
     9001 aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct gccaccattt
     9061 accggacacg acgaaaaaag ccagcgcaac taccatctcg cgcttcgtgg caacagcctg
     9121 aatccgatga tcgacgcggc cacgccgcta ctcggtatgg taatgcgcct ttcaaccatg
     9181 aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt tcaggccgtg
     9241 gagcaactgc ttcaggagca gggctacgaa ccgggcgtga ttatctcgtt tcgctacatc
     9301 ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcaaacaa gaacgagtgg
     9361 atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga gaaagttttc
     9421 atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct ggaatttctc
     9481 tggatctgtt tttctctggg atttcgcggg cgctacaaag tggcagcaca ggatcagggg
     9541 gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg cggcgatgcc
     9601 cctttcccgc tgttacacca ggacaaaaaa acccagggcg ggcgttatca gctcatcagt
     9661 cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt gttttacctg
     9721 ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct aaacaaatta
     9781 cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa cggctgaacc
     9841 tgttctcccg ccaggcgctg gagatggccg cctctgaatg tatgagtcag caggcagcgg
     9901 aaattaccgt cagccatgtg ctcattcaga tgctcgccat gccacgcagt gacctgcggg
     9961 ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgctg acggtagaga
    10021 actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg ctggttgagt
    10081 ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca gcacagcgaa ctgcgcggcg
    10141 gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta tataccgcct gctgccgccc
    10201 ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag tggacacagg
    10261 agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca atgacggacg
    10321 cctctgacac cctgcttgcc cgctatgcca aaaacatgac cgcagacgcc cgtaacggca
    10381 ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac attctctgcc
    10441 gccgccgtaa aaacaacccg gtggtggtgg gcgaagcggg cgtgggcaaa agcgcactga
    10501 ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg aaaaacaccg
    10561 atatcatgac ccttgacttg ggcgcattgc aggccggggc gtcggtgaag ggtgaattcg
    10621 aaaaacgttt caaagggctg atggcggagg tcatttcctc cccggtgccg gtcattctgt
    10681 ttatcgacga agcacatacc ctgattggcg cgggcaacca gcagggcggg ctggatatct
    10741 ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc gccaccacct
    10801 ggagcgagta caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc ttccagttgg
    10861 tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgcggt ctgtcggcgg
    10921 tctatgaaca gtctcacggc gtgctgattg atgatgacgc cttgcaggcc gctgcgacat
    10981 taagcgagcg ttatctctcc gggcgtcagt taccggacaa agcgattgat gtgctggata
    11041 ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc tcggcgctga
    11101 ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag cttcgcatcg
    11161 gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat gaaacgctga
    11221 cggcgctgga tgaactggaa gcggcctggc accagcagca gacgctggtc cgggagatta
    11281 tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg ttgccggacg
    11341 cagataccgt ggaggatacg cag
//
LOCUS       DAZRVY010000127        11295 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZRVY010000127 DAZRVY010000000
VERSION     DAZRVY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.127"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHXOX_003417"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHXOX_003417"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR7006799.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHXOX_003418"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHXOX_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR7006800.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHXOX_003419"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHXOX_003419"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR7006801.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHXOX_003420"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHXOX_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR7006802.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHXOX_003421"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHXOX_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR7006803.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHXOX_003422"
     CDS             complement(6652..8160)
                     /locus_tag="ACHXOX_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR7006804.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHXOX_003423"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHXOX_003423"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR7006805.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHXOX_003424"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHXOX_003424"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR7006806.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAZRVY010000128        11244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZRVY010000128 DAZRVY010000000
VERSION     DAZRVY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.128"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXOX_003425"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXOX_003425"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR7006807.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXOX_003426"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXOX_003426"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR7006808.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHXOX_003427"
     CDS             complement(2698..3390)
                     /locus_tag="ACHXOX_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR7006809.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXOX_003428"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXOX_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR7006810.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="ACHXOX_003429"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="ACHXOX_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR7006811.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="ACHXOX_003430"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="ACHXOX_003430"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR7006812.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="ACHXOX_003431"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="ACHXOX_003431"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR7006813.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9894..10484)
                     /locus_tag="ACHXOX_003432"
     CDS             complement(9894..10484)
                     /locus_tag="ACHXOX_003432"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR7006814.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            10584..>11244
                     /locus_tag="ACHXOX_003433"
     CDS             10584..>11244
                     /locus_tag="ACHXOX_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7006815.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2906 a         2822 c         2629 g         2887 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atatttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct
     9961 tccccatcga ataccactgt attacacaag aaagtgacat cggcatcttc aataccaaga
    10021 taactgcaca tattttttaa ataatcgctt atattttttt cccagcccat ctggacaaat
    10081 gattctttgt ctcctccaac cagcgccacc caacgaactt tattgaatgg taatttctgc
    10141 ccatctccat aagccagccc attattccat actctgtcaa tatatccttt tagcattgcc
    10201 gggaagctgt accaccagac aggaaaaacc accactaacg tgtcatgttc aagcagctct
    10261 gaataaagct gatgaacctc tggagaataa cgtttatcca tattcttcca gtctggttca
    10321 tcttccggcg tcatcaccgg gttgaaatta cgccgatata aatcaagttc cgtcacctgt
    10381 attttccgct ccattgcccg ctgatggatc gcatcagcaa tatgtgcggt caatgaatca
    10441 tgacgaggat gggcccatac caaataaata cgttcagact gcataatttt acctccacgt
    10501 taagttctgg agcgcacttt acgcaggcgg cataatcatg taaacgtgat gattcaggat
    10561 gagttaatga aggattttca ctaatgcgga tgaatatgtc tgactttgcc actttctttg
    10621 ctgtggcccg taatcaaagc tttcgtgcag cgggcgatga gttaggctta tcctcgtccg
    10681 ccattagcca tagtattaaa acactggaac aacgtcttaa aattcgctta ttcaatcgga
    10741 caacccgaag cgtatcgtta acggaagcgg gttctaatct ctatgaacga ttgcgcccag
    10801 ctttcgacga aattcagatc atgttagatg agatgaatga ttttcgtctg acaccaacag
    10861 gaacgctcaa aatcaatgcc gcccgggtag ctgcacgtat atttttaatg cctttactgg
    10921 ttggtttcac acgtgaatat cctgatatta aagtcgagct cacaactgac gactctcttg
    10981 tcgatatcgt ccaacagggc tttgacgctg gcgttcgact tagtggcatc gtcgaaaaag
    11041 atatgatcag cgtggcgatc ggcccaccag tgaaattatg tgtagcagca acgccagagt
    11101 actttgcaag atatggcaaa ccccgccatc ctcatgatct gcttaatcat caatgtgttg
    11161 tatttcgtta tcccagcggc aaacctttcc actggcaatt tgcaaaggag caggaaattg
    11221 ctgtcgcagg gaatatcatt ctgg
//
LOCUS       DAZRVY010000129        11054 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZRVY010000129 DAZRVY010000000
VERSION     DAZRVY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.129"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            91..852
                     /gene="adiY"
                     /locus_tag="ACHXOX_003434"
     CDS             91..852
                     /gene="adiY"
                     /locus_tag="ACHXOX_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR7006816.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            989..2326
                     /gene="adiC"
                     /locus_tag="ACHXOX_003435"
     CDS             989..2326
                     /gene="adiC"
                     /locus_tag="ACHXOX_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR7006817.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2430..4073
                     /gene="eptA"
                     /locus_tag="ACHXOX_003436"
     CDS             2430..4073
                     /gene="eptA"
                     /locus_tag="ACHXOX_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR7006818.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4070..4738
                     /gene="basR"
                     /locus_tag="ACHXOX_003437"
     CDS             4070..4738
                     /gene="basR"
                     /locus_tag="ACHXOX_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR7006819.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4739..5839
                     /gene="pmrB"
                     /locus_tag="ACHXOX_003438"
     CDS             4739..5839
                     /gene="pmrB"
                     /locus_tag="ACHXOX_003438"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR7006820.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5815..5904)
                     /gene="pmrR"
                     /locus_tag="ACHXOX_003439"
     CDS             complement(5815..5904)
                     /gene="pmrR"
                     /locus_tag="ACHXOX_003439"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR7006821.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6016..7518)
                     /gene="proP"
                     /locus_tag="ACHXOX_003440"
     CDS             complement(6016..7518)
                     /gene="proP"
                     /locus_tag="ACHXOX_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR7006822.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7782..8648)
                     /locus_tag="ACHXOX_003441"
     CDS             complement(7782..8648)
                     /locus_tag="ACHXOX_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR7006823.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8645..10873)
                     /gene="crfC"
                     /locus_tag="ACHXOX_003442"
     CDS             complement(8645..10873)
                     /gene="crfC"
                     /locus_tag="ACHXOX_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR7006824.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2775 a         2878 c         2700 g         2701 t
ORIGIN      
        1 ttatattcat catacaatta tataaccatt tcccggtatc gctttgcttt agcgagaact
       61 ggtcttttat tcgcactcag gagtacatgt atgaggattt gcagcgacca accttgtatt
      121 gttttattga ctgaaaaaga tgtctggata agggtgaatg ggaaagaacc tattagcctt
      181 aaagctaacc atatggcgtt attaaattgt gaaaataata ttatcgacgt ctcctctctt
      241 aacaacactt tggttgctca tattagtcac gacatcatca aagattacct ccggtttctg
      301 aataaagatc tctcgcaaat accagtatgg caacgtagcg ctacgcccat actcaccctg
      361 ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac acagcatgat gcccgcagaa
      421 actgagtcag aaaaggaacg aacacgcgca ttattattca cggtgctatc ccgttttctc
      481 gacagtaaaa aattcctttc attaatgatg tatatgttac gtaattgtgt aagtgacagc
      541 gtttatcaaa ttattgaaag cgatattcac aaagactgga atcttagtat ggtagccagt
      601 tgtttatgtc ttagcccaag tctgttaaag aaaaagctga aaagcgaaaa caccagttat
      661 agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa atgaattaat gatggacggt
      721 aaaaatatct ctcaggtatc acagtcctgc ggctacaaca gtacgtcgta ctttatttct
      781 gtctttaaag acttctacgg tatgacgccg ctgcattatg ttagtcagca cagagaacga
      841 actgtcgcct gatttttaac cttaacgaag agctatatta ataacggcat cagcgataac
      901 ccggtcgata ataattcaac tatcgaatgc aggcgtatga tatgacgtaa tttattgtca
      961 cgaagctcgc cttcgcagga gtttaattat gtcttcggat gctgatgctc acaaagtggg
     1021 cttaatcccc gtcaccctga tggtgtcggg gaatattatg gggtcaggtg tttttctgtt
     1081 acctgcaaac ctggcctcca ctggcgggat tgccatttat ggatggttgg tgacgattat
     1141 cggtgcgctg gggctctcga tggtatacgc caaaatgtcg ttcctcgatc caagtcctgg
     1201 tggttcttac gcttacgccc gccgctgctt tggcccgttt ctcggttatc aaaccaacgt
     1261 cctctactgg ctggcctgct ggatcggcaa tatcgctatg gtggtcattg gcgtaggata
     1321 tttaagttac ttcttcccga ttctgaaaga tccattggta ttaaccatca cctgcgtcgt
     1381 ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg aaaatgatca cccgtgtgca
     1441 ggccgttgcc accgtgctgg cgctgatccc catcgtcggg attgccgtat ttggctggtt
     1501 ctggttccgt ggtgaaacct atatggcggc atggaacgtc agcggcctgg gcaccttcgg
     1561 tgcgattcaa agtaccctta acgttacgct gtggtcgttc atcggtgtgg aaagtgcctc
     1621 cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc cctatcgcca ccattggtgg
     1681 ggtattgatt gccgccgttt gctatgtgct ctccaccacc gcgattatgg ggatgatccc
     1741 taatgccgca ctgcgcgttt ctgcctcgcc attcggtgat gccgcacgga tggcgttggg
     1801 tgacaccgct ggggccattg tttccttctg cgcagctgcg ggttgcttag gttcactggg
     1861 cggctggacg ttgctggcgg gtcaaacggc gaaagccgct gccgatgacg gactgttccc
     1921 accgattttt gcccgtgtaa ataaagcggg tacgccagtg gcggggctga ttatcgtcgg
     1981 tattttgatg accatcttcc agctcagtag catttcgcca aacgcaacca aagagttcgg
     2041 tctggtatct tccgtgtcgg tcatctttac actggtgcca tatctttaca cctgtgcggc
     2101 gttactgctg ctcggccacg gtcactttgg taaagcacgc ccggcatatc tggctgttac
     2161 taccattgcc ttcctctact gcatctgggc cgtggtgggg tccggggcga aagaggttat
     2221 gtggtcattt gtcaccctga tggtcatcac cgccatgtat gcgctgaatt acaaccggct
     2281 acataaaaac ccgtatccct tagatgcacc aataagcaaa gattaattcc ccttaatcca
     2341 gcaaacataa aagccaacct taagaactta aggttggctt aattttgctt tgcgagcata
     2401 tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg cctactaaaa agcccctctt
     2461 tgaatttact cgcctggcta ttgttggccg ctttttatat ctctatctgc ctgaatattg
     2521 ccttttttaa acaggtgttg caggcgctgc cgctggattc gctgcataac gtactggttt
     2581 tcttgtcgat gccggtcgtc gctttcagcg tgattaatat tgtcctgaca ctaagctctt
     2641 tcttatggct taatcgacca ctggcctgcc tgtttattct ggttggcgcg gctgcacaat
     2701 atttcataat gacttacggc atcgtcatcg accgctcgat gattgccaat attattgata
     2761 ccactccagc agaaagttat gcgctgatga caccgcaaat gttattaacg ctgggattca
     2821 gcggcgtgct tgctgcgctg attgcctgct ggctaaaaat caaacctgcc acctcgcgtc
     2881 tgcgcagtgt tcttttccgt ggagccaata ttctggtttc tgtactgctg attttgctgg
     2941 tcgccgcact gttttataaa gactacgcct cgttgttccg caataacaaa gagctggtga
     3001 aatccttaag cccctctaac agcattgttg ccagctggtc atggtactcc catcagcgac
     3061 tggcaaatct accgctggtg cgaattggtg aagacgccca ccgcaacccg ttaatgcaga
     3121 acgaaaaacg taaaaatttg accatcctga ttgtcggcga aacctcgcgg gcggagaact
     3181 tctccctcaa cggctacccg cgtgaaacta acccgcggct ggcgaaagat aacgtggtct
     3241 atttccctaa taccgcatct tgcggcacgg caacggcagt ttcagtaccg tgcatgttct
     3301 cggatatgcc gcgtgagcat tacaaagaag agctggcaca gcaccaggaa ggcgtgctgg
     3361 atatcattca gcaagcgggc atcaacgtgc tgtggaatga caacgatggc ggctgtaaag
     3421 gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct gaatctgcct ggtcagtgca
     3481 tcaacggcga atgctatgac gaagtgctgt tccacgggct ggaagagtac atcaataacc
     3541 tgcagggtga tggcgtgatt gtcttacaca ccatcggcag ccacggtccg acctattaca
     3601 accgctatcc gccgcagttc aggaaattta ccccaacctg cgacaccaat gagatccaga
     3661 cctgtaccaa agagcaactg gtgaacactt acgacaacac gctggtttac gtcgactata
     3721 ttgttgataa agcgattaat ctgctgaaag aacatcagga taaatttacc accagcctgg
     3781 tttatctttc tgaccacggt gaatcgttag gtgaaaatgg catctatctg cacggtctgc
     3841 cttatgccat cgccccggat agccaaaaac aggtgccgat gctgctgtgg ctgtcggagg
     3901 attatcaaaa acggtatcag gttgaccaga actgcctgca aaaacaggcg caaacgcaac
     3961 actattcaca agacaattta ttctccacgc tattgggatt aactggcgtt gagacgaagt
     4021 attaccaggc tgcggatgat gttctgcaaa cttgcaggag agtgagtgaa tgaaaattct
     4081 gattgttgaa gacgatacgc tgttattgca gggactgatt ctggcggcgc aaaccgaagg
     4141 ctacacgtgc gatggcgtaa caaccgcgcg gatggcggaa caaagccttg aggccggtca
     4201 ttacagcctg gtggtactgg atttagggtt acccgacgaa gatggactgc attttctcgc
     4261 ccgtatccgg cagaaaaaat acaccctgcc ggtactgatc ctcaccgctc gcgatacgct
     4321 gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac tatctggtga agccttttgc
     4381 gctggaagag ttacacgccc gtatccgcgc cctgctacga cgccataata atcagggcga
     4441 aagtgagctg attgttggca atctgacgct gaatatgggt cgccgtcagg tatggatggg
     4501 cggtgaagag ttgattctga cgcccaaaga atatgctctg ctgtcacggt taatgctcaa
     4561 agcaggcagt ccggtacatc gggaaattct ctacaacgac atctataact gggacaatga
     4621 accctcgacc aacaccctgg aagtgcatat ccacaatctg cgcgacaaag tgggcaaagc
     4681 ccgtatccgc accgtgcgcg gctttggcta tatgctggtc gcgaatgagg aaaactaatt
     4741 gaatctgatg cgttttctgc gccgaccaat atcgctgcgc caacggctga tattgaccat
     4801 cggggccatt ttgttggtgt ttgagctgat cagcgtcttc tggctatggc atgaaagtac
     4861 cgagcagatt cagctgtttg agcaggcgct acgcgacaat cgcaacaacg atcgccacat
     4921 catgcgtgag atccgcgagg cggtcgccag cctgattgtc cccggcgtct ttatggtcag
     4981 cctgacgcta tttatctgct atcaggcggt acgccgcatc acccgcccgc tggcggagct
     5041 gcaaaaagag ctggaagcgc gcaccgccga caacttaacg cccattgcca ttcacagcgc
     5101 caccctcgaa atcgaagcgg tggtttcggc gttgaacgat ctggtcagtc gcctgacgag
     5161 cacgctggat aacgaaaggt tatttaccgc tgacgtcgcg cacgaactgc gaacgccact
     5221 ggcgggggtg cgtttgcatc tggaactgct ggcgaaaacg catcacattg atgtagcacc
     5281 gttagtggca cggcttgatc agatgatgga gagcgtctcc cagctgctgc aactggcgcg
     5341 tgccggacag tcattttctt ccggtaatta tcaacatgta aaactgctgg aagatgtcat
     5401 tctcccctcg tatgacgaac tcagtaccat gctcgaccag cgacagcaaa ccctgctatt
     5461 gccagagagc gccgccgaca tcaccgttca gggtgatgca accctgctgc ggatgttatt
     5521 gcgaaacctg gtagaaaacg cccatcgtta cagcccacaa ggcagcaaca ttatgattaa
     5581 gctgcaagaa gatggcggag cggtcatggc cgttgaagat gaaggaccgg gtattgatga
     5641 gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg gacagccgtt atggcgggat
     5701 tggtctgggg ttaagtattg tcagccgcat tacacagttg catcacgggc agtttttcct
     5761 gcaaaaccgg caagagactt ccggcacgcg ggcctgggta cggctgaaga aagatcagta
     5821 cgtagcaaac cagatataaa gaaagctgct gaccaccagc acactgaaca cggtagttaa
     5881 actttcataa acacggtttt tcattgcact ctcctccgtt aacctggagg agagtatgcg
     5941 cgtcagagat taagtgaacc ttaagagttc aattggcctg ataagacagc gtcacatcag
     6001 gccatccgtt tcagcttatt catcaattcg cggatgttgc tgcaccaggc gggtacgttt
     6061 cgcctgcaaa tcggcaatct cgtggtcaat atcatcgatt ttctgctcga tattatcgta
     6121 atgctcgacg agaatttcct tcgcttcctg tatatctgac gccgccggtg tcgcaccttt
     6181 caacggacga tttgccgtct ctttcatggt tacgccggtg attaaaccaa tcaccgccac
     6241 taccatcagg taataggcag gcatcatcag attctgcgag ctttcgacca gccaggccgc
     6301 cagtgttggc gtcagaccgg caaccagcac cgaaatatta aatgccgccg ccagcgcgct
     6361 gtaacggata tgcgtcggga acatcgctgg caaggtagaa gccataacgc ccgtaaagca
     6421 gttaaggatc accgccagca tcagtaaccc ggcaaaaatc aggccgatga cgttactgtt
     6481 aatcagaata aacgccggga tcgccaacac aaacagggca acactaccaa gtagcacaaa
     6541 cggacgacgg ccaaaacggt cactcagcaa gcccatcacc ggctggacaa acagcatacc
     6601 gatcataatg gcgataataa tcagcacccc gtggtcttcg gagtaatgca ggttatgcga
     6661 caaataactc ggcatatagg tcagcaacat gtagtaagtc acgttggtgg caattaccag
     6721 accaatacat gtcaacaggc tgcgccagta tttagtggca atctctttaa acgagacttt
     6781 cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt ttatcaacat gctgctggaa
     6841 cgccggagtt tcttccagcg catggcgcag gtaaagcccg ataatcccta acggcagagc
     6901 aataaagaac ggaatacgcc agccccagtc gaggaagttc gcttcgccga caatggtcga
     6961 aattaacacc accacgcccg cacccagcac aaacccggca atagaaccga agtccagcca
     7021 gctgcccata aagccacgtt tacggtcagg ggagtattcc gcaacaaata tcgacgcccc
     7081 ggtatattca ccgccgaccg agaaaccttg tgccatctta cagatcaaca gcagaatcgg
     7141 tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca atacagaacg tactgatcga
     7201 cataatcaca atagtgatag cgaggatctt ctggcgacca tatttatcgc ccaacatacc
     7261 aaagaagagc ccgccaagcg gtcgaatcag aaagggaacg gagaaagtgg caagtgcagc
     7321 aaccatctgc acgctggggt cagcccccgg gaaaaaaact ttacctaatg cgtaagcaac
     7381 aaaaccataa acaccaaaat cgaaccattc cattgcatta cccagtgatg ctgcggtaat
     7441 ggctttacgc agtttaccgt catcaataat ggtgacatca cgaagggtaa tcggttttac
     7501 ttttttcctt ttcagcatag ctttcctcgc agagttggcc catttccgca aacgccagaa
     7561 aaatcagcgc gacggccgca aaaggggagc gggaattgca ggatgcaatc tctgtaacaa
     7621 gcgtagcggg tttgcttaca ccctccggtt aaggaatgta caatcaaacc agttgacgcc
     7681 tgaattgggc agttaatgac ctttttaccc taccagtgtt taaatttgtg atcaacttca
     7741 cacatggatt tcgtttagga ctcattgatg taactgatta tttacgccaa atttacagag
     7801 aagatttcct cgaccattgc ccccaccaaa cgctgcgctg aacagagtcg tggcatcccc
     7861 agcgcgactg tttgccagcg ttgggtcact gaccagcaaa cgggctgttt atctgccgtg
     7921 gaccactttc ccagccaact taaaaaatca gcatgctccc ctcccgaaga ggcaaaccac
     7981 tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac tctctgccag atgttgtgtc
     8041 gctttctcgc gcaaatcacc cagtaacaac accaccgctt gcgggcttcc cagacggcag
     8101 gcatcgccaa atgcacccca gcgcaactcc tccagaacga taaaacagcg cccaggcaac
     8161 gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa tttgctcgct ctgatcacct
     8221 gcctgcacca gtaggcctcg ttgcgttaac gccttttcct tccagcgaca acgggctgaa
     8281 aaccactcat acaaggcggc aatttgctgg gggagctccg ggaaagcaga gtgctggcgg
     8341 tgatccattt tttgcagcag cgccagatgc tgcgcgatct gcgtcagcgc cagatgaccg
     8401 ggatccagca tcgacgccag tttttccacc attgtcagtc gcaggctaag cggcgcattt
     8461 aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg aaagaagttc ctgcatcagt
     8521 cgttcacgaa actgctgctg atgggcttgt ggaaggcggg catcatcgcc ctgcgccaga
     8581 tccactaaaa agcggggata aaccgactcc agcacgcgac cggggccgtc cagtaacgtc
     8641 ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc atcgcgtaac agctgggcgt
     8701 cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa ttgctgctgc atcagcctga
     8761 ccaccgattc attttgttga cgcagagcca ggctttcgcg taaacgcgtc tgtaattccg
     8821 tcaaacatga agcgaactca gcgcaaaaag tattcatgcc tgccgtaaca gattcatcga
     8881 cctgctctgc cagaacttta gccatttgtt ggcaataaag atcgacttct gcgcttaaag
     8941 ctcgttgcaa cacactgtaa tcaaccgtct ctgtcgggga tttctcattt ccccgtcccc
     9001 agtcgggctg attcaaccag cgcgaaaaag tctcacgcac aacgcctaaa cgcgtgctct
     9061 gctcgtccgt tgcatcctgg cgcgaaatga cttcactgaa cagctggcga gtgttgacgt
     9121 ggggaactac tccgtgaaaa acaggaaaat gaaacccagg acgaaaccct gactcgctca
     9181 attccatttt gacttgttgc tcaatgggcc gaataacatc ggttaacacc cggcaaaggg
     9241 tggattcaag ctcggcaaaa cgcagcgtaa agtcgcgact gatggtgttc tgcgccgtct
     9301 gtaacagtgt ctcacagcag gtacgaatct cgcttaacgg ctccgaatca tcctgaaaca
     9361 aggcggctaa ctgcgcattc agcgcatctt gctgttgacg cagaaagtgg ttggcggagg
     9421 tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga ttgagttgca
     9481 atagctgcaa actttcttcg acctgatgga tattttgccg caattgttca caagcgacgt
     9541 ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa ttcaacagtt
     9601 tatgcgcagc agatcgcaga gcatacaacg aggcgttagc gtaagcggca tgaagcaacg
     9661 cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca tgacgaatat
     9721 gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca tgggcaaaat
     9781 cttccaccca gcgttgttgc tctggcgctg gtaacttacc gttgttggct aactcatggc
     9841 gcgctcgatt tgccaagtag ccccacatcg acgacaccgg aaatatctgc tgtggcgtaa
     9901 tacagccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg tcgtcactgt
     9961 tacgatcctg ttgatcgaac ttattgacca gcacatacag tggcaccgat tgccccaccg
    10021 ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt cagttgcgta taatccagca
    10081 ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc agatgcggtt
    10141 gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga taactctcca
    10201 gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg gcggcgtaag
    10261 cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg cactaaatca ttcaaacttt
    10321 tcagacaatg aaaaataggc tgggcaccca gataatattt ttcgaaagcg acgccatttt
    10381 cgatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg gtcagatgct
    10441 taatatcgca atcacgcagg cgctgttgta attgttgaat taaacaatcg attggcgcga
    10501 catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt atggcgaata agcgtcggca
    10561 gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg gcattaatgg
    10621 tggttgattt ccctgctttc atagtaccga caatcgccag caccatttcc agtcgggaaa
    10681 ttttactcaa ctcattattc agcatcgcgt gacgttcggc gatattaggc tgactccagg
    10741 gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag aggcattttt tccagtaact
    10801 gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac tcatacaggg
    10861 tctgtgtgta catagaaaat tcttccttaa agcaaatttt gttactttta tttagccaga
    10921 ttgtttttga gttctgtttt tggcttttat aattactgca agaaataatt ttatatttag
    10981 tgtgttgttt tttatcagaa taaataacgt cttctgatgc gtttaaaacg tcagaaagat
    11041 aaaaatatca tgtg
//
LOCUS       DAZRVY010000130        10939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZRVY010000130 DAZRVY010000000
VERSION     DAZRVY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.130"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHXOX_003443"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHXOX_003443"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR7006825.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHXOX_003444"
     CDS             complement(643..882)
                     /locus_tag="ACHXOX_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR7006826.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHXOX_003445"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHXOX_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR7006827.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHXOX_003446"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHXOX_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR7006828.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHXOX_003447"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHXOX_003447"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHXOX_003448"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHXOX_003448"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHXOX_003449"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHXOX_003449"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHXOX_003450"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHXOX_003450"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR7006829.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHXOX_003451"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHXOX_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR7006830.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHXOX_003452"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHXOX_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR7006831.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /gene="yecU"
                     /locus_tag="ACHXOX_003453"
     CDS             7047..7223
                     /gene="yecU"
                     /locus_tag="ACHXOX_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR7006832.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /gene="yecF"
                     /locus_tag="ACHXOX_003454"
     CDS             7210..7434
                     /gene="yecF"
                     /locus_tag="ACHXOX_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR7006833.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHXOX_003455"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHXOX_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR7006834.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHXOX_003456"
     CDS             complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHXOX_003456"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR7006835.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHXOX_003457"
     CDS             complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHXOX_003457"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR7006836.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHXOX_003458"
     CDS             complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHXOX_003458"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR7006837.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2713 a         2838 c         2578 g         2810 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcttggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     8281 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
     8341 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     8401 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     8461 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
     8521 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
     8581 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     8641 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     8701 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
     8761 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     8821 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     8881 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     8941 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
     9001 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     9061 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     9121 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     9181 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     9241 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     9301 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
     9361 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
     9421 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     9481 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     9541 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
     9601 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     9661 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     9721 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
     9781 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     9841 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     9901 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     9961 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    10021 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    10081 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    10141 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    10201 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    10261 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    10321 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    10381 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    10441 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    10501 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    10561 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    10621 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    10681 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    10741 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
    10801 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
    10861 tgcagtggca taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa
    10921 aacgcgtaaa taaaaaagg
//
LOCUS       DAZRVY010000131        10833 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZRVY010000131 DAZRVY010000000
VERSION     DAZRVY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.131"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..277)
                     /locus_tag="ACHXOX_003459"
     CDS             complement(<1..277)
                     /locus_tag="ACHXOX_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7006838.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRS"
     gene            complement(444..1184)
                     /locus_tag="ACHXOX_003460"
     CDS             complement(444..1184)
                     /locus_tag="ACHXOX_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR7006839.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHXOX_003461"
     CDS             complement(1277..1912)
                     /gene="argO"
                     /locus_tag="ACHXOX_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR7006840.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHXOX_003462"
     CDS             complement(2051..2911)
                     /gene="mscS"
                     /locus_tag="ACHXOX_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR7006841.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHXOX_003463"
     CDS             complement(3102..4181)
                     /gene="fbaA"
                     /locus_tag="ACHXOX_003463"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR7006842.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHXOX_003464"
     CDS             complement(4396..5559)
                     /gene="pgk"
                     /locus_tag="ACHXOX_003464"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR7006843.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHXOX_003465"
     CDS             complement(5609..6628)
                     /gene="epd"
                     /locus_tag="ACHXOX_003465"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR7006844.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7000..7431
                     /locus_tag="ACHXOX_003466"
     CDS             7000..7431
                     /locus_tag="ACHXOX_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR7006845.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7454..8032
                     /locus_tag="ACHXOX_003467"
     CDS             7454..8032
                     /locus_tag="ACHXOX_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR7006846.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8033..8740
                     /locus_tag="ACHXOX_003468"
     CDS             8033..8740
                     /locus_tag="ACHXOX_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR7006847.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8728..9405
                     /locus_tag="ACHXOX_003469"
     CDS             8728..9405
                     /locus_tag="ACHXOX_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7006848.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9399..10076
                     /locus_tag="ACHXOX_003470"
     CDS             9399..10076
                     /locus_tag="ACHXOX_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7006849.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10048..10761)
                     /locus_tag="ACHXOX_003471"
     CDS             complement(10048..10761)
                     /locus_tag="ACHXOX_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR7006850.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10758..>10833)
                     /locus_tag="ACHXOX_003472"
     CDS             complement(10758..>10833)
                     /locus_tag="ACHXOX_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7006851.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2879 c         2699 g         2675 t
ORIGIN      
        1 gtgaacgacg ccctgagtta tggatttcat tatcaatcgc agaaagtcgt tgataaaccg
       61 ggaacagttt ttgatacagt tcctgaccgg ctttattgag gctgatatta ttatctttgc
      121 gggtaaatag cgtatagcca atttgttctt ccagcgcggc aatgctttta ccaaatggcg
      181 aagccgtcat atggattttt tctgccgccc gggcaatatt attggtttgc gctaataaaa
      241 tgaaattgcg catctttttc gaaataaaaa tgtccatccc ccctccccgc atccttgtcc
      301 gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata ggtgattacg
      361 tcatggtttt acagaaaatt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
      421 acgaatacgt cgggctacaa gtattattgt gctgcaggtg ttttagcggg ttgttgatcc
      481 acaggttcta actggaagac cacatcgacc tgatcatcaa actgaatagc ggcctgctcg
      541 taagtttcct gggcagacac cggcgcggca tcggctttca tcatccgcac cattgggctg
      601 ggctgatagt tggaaacatg gtagcgcacg ctatataccg gccccagttt acgatgaaag
      661 ccgttcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
      721 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
      781 gccttcagcg cgccatccag caaggaattc agtttgtcta actgacggag cgtgacttcc
      841 accgttctca cagcgcggta gcctttcagg atacttttac catcctgata atcataatct
      901 ggctgggtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagttcaagg
      961 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggctgtagc ggcatccttc
     1021 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacacttgcc
     1081 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
     1141 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
     1201 taattgactt gcccaaaatt caggacctct ggaggcaagc ttagcgcctc tgttttattt
     1261 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
     1321 gctgcaaggc tataaaccac atgacacatc ccactaccag attgataatg cgctgtgatt
     1381 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
     1441 ggaaagaggc gctaattgtc ccgagcgcaa accaacgttt tggttcgacg tcaagctgcc
     1501 cgccaaggct gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
     1561 ctgccagcat ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta
     1621 actcaatatt actactcatc gctgttttaa aagcgccaaa accataccac agcaagaagg
     1681 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
     1741 caccaaaaat cccggcgcaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
     1801 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
     1861 gcagaatcat agctgcccca agagcaatac cttgaaagta ataagaaaac acgttggata
     1921 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
     1981 taatattaat tggcaatcag aataactaat aagagagagg ccagagagta ttatctggcc
     2041 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
     2101 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
     2161 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
     2221 atgcaccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
     2281 actggataat attggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
     2341 caataataaa ttcgttacgg cgaactggct cgcgagagaa gttaataata tttccggcaa
     2401 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
     2461 aaatctgcac actcagcaca gtaccggcta cgccgccgag atcaacatat tctccggcac
     2521 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagaaagt gacccctgca
     2581 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
     2641 cacccacgcg tcccagggca gcgattagcg taaaggcgat aataccgtaa cggactaatg
     2701 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
     2761 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
     2821 tgtttactgc ataacttagc agcagcgcct ggttagctac cagccagctt cccgcgccgt
     2881 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
     2941 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
     3001 aattttgcca aatagatcac agatttctca tctgaatcac cttgcttttg gcgaaaaaaa
     3061 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
     3121 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc
     3181 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac
     3241 tgcccctgca gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc
     3301 cattgggtat cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt
     3361 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
     3421 ttgtgtttct tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg
     3481 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
     3541 ctgattttgc tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
     3601 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg
     3661 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
     3721 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
     3781 gtagctgcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
     3841 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga
     3901 acgtgatgcg caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc
     3961 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
     4021 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
     4081 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
     4141 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
     4201 cgggccttat actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
     4261 gtcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
     4321 ttatttagag tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
     4381 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
     4441 tttaccttcc acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
     4501 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
     4561 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
     4621 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
     4681 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
     4741 agatttcagg gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
     4801 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
     4861 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc
     4921 aacaatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
     4981 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
     5041 cgcgtccagt tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat
     5101 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
     5161 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
     5221 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
     5281 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
     5341 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
     5401 tttcgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
     5461 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
     5521 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
     5581 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
     5641 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
     5701 tcaggtgtgc gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt
     5761 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
     5821 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
     5881 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa
     5941 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
     6001 gaatgatcga ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa
     6061 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
     6121 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
     6181 cgatgcggtg ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga
     6241 gatcgttact gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt
     6301 cgccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
     6361 cacgccaggg gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa
     6421 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
     6481 atttcaacaa atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa
     6541 tttccgcccg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
     6601 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
     6661 tgccagacag agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac
     6721 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
     6781 gtggaataaa aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt
     6841 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
     6901 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
     6961 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
     7021 tattcttccc ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc
     7081 cagctacacg cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc
     7141 ggtagcctga ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac
     7201 ggtacattcg aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat
     7261 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
     7321 gtgcggacca cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa
     7381 ccttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actactttca
     7441 aagaggtttt cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat
     7501 atctatcgcc atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt
     7561 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct
     7621 gaccggctta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
     7681 tgcacccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
     7741 tcgcaccctg ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt
     7801 cgctgtaccg ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt
     7861 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
     7921 aggcgcgaac ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca
     7981 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
     8041 atttacctca ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca
     8101 gaccattttg ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac
     8161 gcggcggcgg gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct
     8221 ggtacacggt ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg
     8281 gacgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
     8341 gatgcagtac gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc
     8401 aggcgtcgct tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt
     8461 agcgattatt catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca
     8521 gcgtttacgt gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct
     8581 ggctgtccgt ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac
     8641 gttgtgggca ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct
     8701 gatgctaaca gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg
     8761 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
     8821 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg
     8881 tatccggact ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt
     8941 gggcatctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
     9001 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
     9061 ctgtgtcttg ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag
     9121 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg
     9181 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt
     9241 cgcctgacgc cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
     9301 cgtggttcgc tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag
     9361 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
     9421 cttatcgctg gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac
     9481 aaggtgaatg gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
     9541 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga
     9601 aaaacctgaa aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa
     9661 accaactttt tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat
     9721 gcccggcgga tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag
     9781 acactgccaa ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg
     9841 cctgcctgga agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg
     9901 acgaaaattg gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga
     9961 gcgtggtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    10021 gactggaaga tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt
    10081 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    10141 gtcggtacgg tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg
    10201 ccccaccaga cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca
    10261 aaattgcgca agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat
    10321 gacgagtggt gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta
    10381 ctgcggccac gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac
    10441 gtcaaatgtc tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt
    10501 gtaatggtga aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg
    10561 cacgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    10621 aaaaaccacc gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa
    10681 cggtttatgc acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt
    10741 caccgttaat tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    10801 agcgccgttt tgaccattcc gctaaaacgc gcc
//
LOCUS       DAZRVY010000132        10711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZRVY010000132 DAZRVY010000000
VERSION     DAZRVY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.132"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..625
                     /gene="rfaY"
                     /locus_tag="ACHXOX_003473"
     CDS             <1..625
                     /gene="rfaY"
                     /locus_tag="ACHXOX_003473"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR7006852.1"
                     /translation="IFDEILAYNFKTVKVLRNINDTKVSLIDTKYGRYVFKVFAPKTK
                     RNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYV
                     EGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSC
                     TAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
     gene            651..1676
                     /locus_tag="ACHXOX_003474"
     CDS             651..1676
                     /locus_tag="ACHXOX_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFR7006853.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            1761..2744
                     /locus_tag="ACHXOX_003475"
     CDS             1761..2744
                     /locus_tag="ACHXOX_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR7006854.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            2790..4043
                     /locus_tag="ACHXOX_003476"
     CDS             2790..4043
                     /locus_tag="ACHXOX_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR7006855.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(4113..5084)
                     /gene="rfaC"
                     /locus_tag="ACHXOX_003477"
     CDS             complement(4113..5084)
                     /gene="rfaC"
                     /locus_tag="ACHXOX_003477"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR7006856.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(5088..6134)
                     /gene="rfaF"
                     /locus_tag="ACHXOX_003478"
     CDS             complement(5088..6134)
                     /gene="rfaF"
                     /locus_tag="ACHXOX_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR7006857.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(6144..7076)
                     /gene="rfaD"
                     /locus_tag="ACHXOX_003479"
     CDS             complement(6144..7076)
                     /gene="rfaD"
                     /locus_tag="ACHXOX_003479"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR7006858.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNSQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            7290..8486
                     /gene="kbl"
                     /locus_tag="ACHXOX_003480"
     CDS             7290..8486
                     /gene="kbl"
                     /locus_tag="ACHXOX_003480"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR7006859.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            8496..9521
                     /gene="tdh"
                     /locus_tag="ACHXOX_003481"
     CDS             8496..9521
                     /gene="tdh"
                     /locus_tag="ACHXOX_003481"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR7006860.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            9760..10662
                     /locus_tag="ACHXOX_003482"
     CDS             9760..10662
                     /locus_tag="ACHXOX_003482"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR7006861.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         2913 a         2296 c         2468 g         3034 t
ORIGIN      
        1 aatctttgat gaaatactgg cctataattt taaaacagta aaagtattac gtaatattaa
       61 tgataccaaa gtatcattga ttgatacgaa atatggtcgc tatgttttta aagtctttgc
      121 cccgaaaacg aaaaggaatg aacgtttttt aaaatctttt gtaaaaggtg actactatca
      181 gaatttgatc gttgaaaccg atcgtgtcag aagtgcaggt cttacttttc ctaatgattt
      241 ttattttctg gctgagcgta aaattttcaa ctatgccagt gtttttatca tgcttatcga
      301 gtatgtcgag ggcgtagagc tcaatgatat gccgatcatt ccagaagatg ttaaagcaga
      361 aattaaggct tcaatggaaa aattgcatgc cttaaatatg ctatcgggcg acccacaccg
      421 gggtaacttt atcgttagta aagatggtgt caggataatt gacctatccg gtaaaagctg
      481 tacggcagag cgtaaagctc gtgatcgtct ggccatggaa cgtcacctgg gtattgccaa
      541 cgaaataaaa gattatggct actattcagt aatctacaga accaaactac gcaaatttat
      601 taaaaaatta aaaggcaaag cgtaaaccac acagtcaaaa cggaaccaac atggatttat
      661 tagctgagag tattactgaa gtcgctgtct ctggggaaat tgctaacacc gatcgtgtgt
      721 taaatatcgc ttacggtatt gaccgtaact ttttatttgg tgcggcagta tctatgcaat
      781 cagttgttat gcataatccg gaccttgcgg ttaagtttca tctctttacc gactacattg
      841 atgaagatta tctacaacgt gttaatgctt ttaccagcaa aaatgctaac gttgaagtaa
      901 gaatttataa agtctccagt gcctttattg atatcttccc cagcctgaaa cagtggtctt
      961 atgcaacatt cttccgttta gttgcgttcc agtatctgag tgaaactatt gaaaatctgt
     1021 tatatatcga tgctgatgtc atctgtaaag gctcattagc tggattgctt gatattaatt
     1081 ttgatgggga taagtttgca gctgttatta aagatgtgcc ttttatgcag gaaaaaccag
     1141 cgaagcgtct ggctatagag ggacttccag ggaattattt caacgccggt gtagtatatc
     1201 tgcagcttga agcatgggcg aaaaatgatt ttatgaataa agccattgct atgctggcaa
     1261 gtgacccgca gcacacgaag tataaatgcc ttgatcagga tattttaaat attctgttct
     1321 ttggtcattg tatttttatt agcggcgatt atgattgctt ttatggcatc gattatgagt
     1381 taaaaaataa aagcgatgaa gattataaaa agaccattac cgatgatact aagctgattc
     1441 attatgttgg cgtaacgaag ccctggaacg actggacgaa ttatccctgc cagaagtatt
     1501 ttaatgaggc ttatcaggct tcttgctgga atgatgtggc gtttattcca gccacgaatg
     1561 aaaagcagta tcaagtgaaa tatcaacatg caaagaaaaa tggtgatacg tttaacgctt
     1621 ttatttactt tattaaattt aaattaaata agtataaaag aaaactattt ggataataat
     1681 aaagatcatg gatgtctgag ttacaataat aattaaggat gagtactatc atattttatt
     1741 agttcaggca agagaacacg atgagcaatg attacccttt agtatccata ataataccga
     1801 cgtataattc atctgattac attactgaaa ctctaacgaa attagaaaaa caaacttacc
     1861 caaattttga aattgttatt gttaatgatg gttctaaaga taacacatca aacgttttga
     1921 gagagtatgg gttaacccac tctcgattaa ttattatcaa taaagaaaat ggcggtgttt
     1981 cgtctgccag gaatacaggt atccgcaagg cgcaaggaca gtttatatgt tttatggatg
     2041 atgatgatga gatagatcct aactatctgc tgaagatgta taccagacaa catgagacgg
     2101 gaggggatgc gatttattgt gggctttatg gccattatat aaaagatggt gttacttact
     2161 cacctataaa tacagagttt aatgaaggat ctttactttt cgactttttt tataaaaagg
     2221 ttagattcca tatagggtgc ttgtttataa gaaaacaact tctggaagag aataatcttt
     2281 tttttgatga agatttacga ctaggagaag atctggattt tatctatcga ctgctaatta
     2341 catgcgatat gtatgcggtt ccatattata tgtataagca taactataga gaaaattcct
     2401 taatgaactc atgtagaacc atcactcatt atcgacatga gtcatttgcg cacgaaagaa
     2461 tctactcttc tgtgatgcag ttatacaaag gtaacaggaa agaagaaatt catacattat
     2521 tgagtcaaaa tagagcttat cataaaactc gttatttatg gaatgttcta cttaatggtg
     2581 attttgaact attaaatcaa ttagttgaaa gcaatgaaaa agaattgaat gattgtaatc
     2641 tttctggcaa gagagataaa agacgagcaa aaatattagc atcaaaaaat tatattctct
     2701 ggaggatggt aagactggta aataggaaaa agaataaacg ttagatcttg cattaaataa
     2761 tccgtcctcg tagcataggt tgaaactata tgtcattttg ttggaatgaa attaattctg
     2821 gtataaagtc tttaattctc atattatgta ttttttcttt gatgactttg tctttatggg
     2881 atgatgttgc aacaaagttt cttcatgcag ctggaattat atctgcattg tattttctaa
     2941 taacaccaaa aaaaacaata actaacaatc ctactttgtt aattttcatc tcattatgtc
     3001 ttttgggaat cataaatatc atctggtatt cacattataa agtttcaggc tctgtttata
     3061 ccaatgcata tcgtggccca atggaaactg ggaaaattgc cttgtgtagt gcttttattt
     3121 tcttggttct ttttgctaaa aatgagttga gaacaaaaat aaaatttggg aaactaattc
     3181 tgttcgcatc cctggcaacg cagttacttt tttttgcgca tgccatgtgg caacatttct
     3241 atttaaacgt tgaccgtgtt gcattatcag cttcccacgc tacaacagca ggctacatca
     3301 tccttttccc ttctttactg gcatcaattc tcattttaaa atccgacttt agacataaaa
     3361 caacattata tacaattaac ttcatgctta gcttatgtgc tgtcatagta actgaaacgc
     3421 gtgcagccat attagtgttt ccattctttg cgttactatt aatcgtaatg gatagttata
     3481 ttaataagcg aattaattat aagttatatt gttttattgc gattgcatta ttagcaggtg
     3541 tattttcttt taaagataca ttgcttatga gaatgaatga cttaaataac gatttagtta
     3601 attattcgca tgataacacc agaacttcag tcggtgcccg tctggcaatg tatgaagttg
     3661 gcttaaaaac atattctcca ataggacaat cactggaaaa acgagcggaa aaaatacatg
     3721 aactagaaga aaaagagcct agattgagtg gcgctttacc ctatatagat tctcatttgc
     3781 ataacgatct catagatacg ttatcaacgc gtggtattcc tggagttgta ttaacaattt
     3841 tagccttttc agcaatactc atatatgcct taagaactgc taaagaacct tatattttaa
     3901 tcttgctttt ttcactactg gtagtaggcc taagtgatgt aatactcttt tctaaaccgg
     3961 ttccgactgc tgtgtttgtc accataatat tgctttgtgc ttattttaaa gcacaatcag
     4021 accaatgttt attagataaa taatgaatat gtgaaataaa atcagcaagg aggctgattt
     4081 tatttcaaaa ttatctatta aggggttgta atttaaatca tagcagcgtt ttcttcaatg
     4141 aatcgcttaa cagcatttgc tgtcaattga gacaactcat tccccggagc cctacaaacc
     4201 atctgattct tcccatatcc accaattaat cccggatcgg ttggtccata aaccgtgata
     4261 ttgggtctat ccagtgccgc cgttaaatgg cttaaccccg tatccaccga cactacaaat
     4321 ttagccccgg ccagcacgcg ggcaacgcct tccagactca tcttcggcaa tacttcaaca
     4381 taagcaaatc cttccgccag tcgtttcgcc cgctcttcct catgcggcgc gccccacgga
     4441 agtttaatcc gtattcctga atcagccagt aaaccaatca attctcgcca gtgttcttcc
     4501 ggccagtgtt tatcatcacg ggtcgtcgca tgaagaaata cggcatattc gccagcatct
     4561 gtaggcaggt tcgtcaggaa atgctgtgca atagcataat cgccctgggt ttgcggtttg
     4621 ctatagccca gacttttggc aaacagttcg cgggtgcgtt ctacggcgtg ctgctgtttt
     4681 gcaatatgat gcttacgatt gtaaaacaga ctggctaaag gttcgcgagc ggtttgccag
     4741 tccatgccat gctttacgcc atgcgccaga cgcgtcacca gcgccgcgct ttttaccagc
     4801 ccctgagcgt cgataactgc gtcatagttc tctgcttgta gcgcttcacg aaacgctttg
     4861 cgttccgctt ttatgggggc cgagaaccag gctttacgcc agcgacgtat tgccacagga
     4921 ataactcgct caacggcggc gtgccaggaa ggaatctgtg cgaacccttc ttccaccacc
     4981 cagtcaaact taatccctgg gattgcctgc tgggcatcag tgagtgcggg caacgtatgg
     5041 agaacatcgc ccatcgacga tgttttaacg atcaaaaccc gcatccgtca ggcttcctct
     5101 tgtaacaata gcgcgttgag ttcttccagt acgcgctggg gagtaatgtc gattaagctc
     5161 tggtgataac cctccgcagc gtcaccttta cgcactttgt gatagccggt aatcaggcgg
     5221 atcacgcgcg ctttatggga tagcggcggt gtgaagtccg ggctactcgg accatacagg
     5281 gcaaccagcg gacgattgag cgccgccgca acgtgcatca ggccagaatc gttagtgaca
     5341 atggctttac aggctgcaat caggataacc gcttgatcaa gctgtgtttc ccccgccagg
     5401 ttccgacacc atgcctgctg ctcggtattc aaagcggcaa gaatctcatt gcccgcttca
     5461 tgatctttcg ccgagccaaa cagaaccacc tgataacctt catcaatcag ctgctttgcc
     5521 agttccgcat agtggtagtg tggccagcgt tttgccggac caaactccgc acccgggcaa
     5581 aaaccaatca tcggacgttc tgatgaaagc gaaaattgat tacaggtata tgatttttca
     5641 ccttcgctca cctgcaattg cggccataac aacggttttg gtagatcttg tgcggtacgc
     5701 atcacgcctt tgtcataggc cagcgcgaca tagcgttcca ccattagcgg ccaggcttct
     5761 ttatcgagca cgcgtaaatc gttgagtaaa ccgtagcgca tctcgccgcg ccagccggtg
     5821 cgatgaggaa tacccgcgaa gaaaggcact aatgcagatt tgaaggagtt gggtaagacg
     5881 taggcgcggt cgtagcgctt ttcacgcagg ctatgaccca gtttgcggcg ttcgccgatt
     5941 tccagcgctc cgtgaccgag aggcatagga atagcttcgt taacttccgg catccgcgat
     6001 aataatggac ggcaccatgc cggtgccatc acatcgatta tcgcctgggg atagcgcgcc
     6061 tggagcgtgc gatagagact ttgcgacatc atcatgtcgc caacccaaga cgggccgatc
     6121 accagtattt tcatgcagag ctcttatgcg tcgcgattca gccaggccat gtattccgtt
     6181 acaccttcag caacggtttt gaacggtttg tcgtaacccg ccgcgcgcag atttgtcaga
     6241 tctgcctgag tgaacgcctg gtagcggcct ttcagcttat ccgggaacgg aatgtattcg
     6301 atctggcctt tcttgtgata agccagcgta gcatcagcta cagcctggaa ggattccgca
     6361 cgaccagtac cgaggttgaa gatgccggaa acgccatttt ccaggaacca cagattcaca
     6421 tcagccacgt cgcccacata gacgaagtcg cgtttgaagt tctcgctacc ttcaaacagc
     6481 ttcggtgatt caccgttgtt aagctgggag ttgagatgga aagcgacgct cgccatgctg
     6541 cctttatggc cttcacgcgg tccataaacg ttgaaatagc ggaagccaac aatctgcgag
     6601 ttagcttcag gcaggatttg gcgaacatat tcatcaaaca ggaattttga gtaaccgtag
     6661 acgttcaacg gtttttcgta ctcgcgggat tcaataaagt cggaggtgcg tccgccgtag
     6721 gtggctgcgg aagaggcata caggaacggg atttcgcgct ccagacagta gtgcagcagc
     6781 tctttggagt attgatagtt gttatccatc atatacttgc catcccactc ggtggtggaa
     6841 gagcacgcgc cttcgtggaa aatcgcttcg acatcgccga actcttcgcc agccataatc
     6901 tggatcagga agtcttcctt atccatatag tccgcgatat ccagatccac caggttcaca
     6961 aacttggtgc cgtctttcag gttgtccacc accagaatat cggtgatgcc tttatcattc
     7021 agggccttaa cgatgttgct gccgataaag cccgcgccgc cggtaacgat gatcataact
     7081 gtaaccttcg aattatggag tcagagacaa tctcagacac gaatacttct atcatatcac
     7141 tacacggcgt tggcttcagc cattcgccaa aagtgagatt gatggacgtt atttatcctg
     7201 aattttgcag aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg
     7261 ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaga attttatcag cagttaacca
     7321 acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg
     7381 cgcagcaagc agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaata
     7441 actatctcgg gctggcgaat tatccggatc tgattgcggc ggcaaaggcg ggaatggatt
     7501 ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag
     7561 agcttgaaca aaaactggcg gcattcctgg ggatggaaga tgcgattctc tactcttcct
     7621 gctttgatgc taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct
     7681 ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct
     7741 atcgctatgc caacaacgat atgcaggagc tggaagcacg tctgaaagaa gcgcgtgaag
     7801 ccggtgcgcg tcatgtgctg attgccaccg atggtgtgtt ctcgatggac ggcgtgattg
     7861 ccaacctgaa gggcgtttgc gatctcgcag ataaatatga tgccctggtg atggtagacg
     7921 actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg
     7981 tgatgggccg ggtcgatatt atcaccggca cgctcggtaa agcgctgggc ggggcttctg
     8041 gtggttatac cgcggcgcgc aaagaagtgg ttgagtggct gcgccagcgt tctcgtccgt
     8101 acctgttttc caactcgctg gcaccggcca ttgttgccgc atccatcaaa gtactggaga
     8161 tggtcgaagc gggtagcgag ctgcgtgacc gtctgtgggc gaacgcgcgt cagttccgtg
     8221 agcaaatgtc ggcggcgggc tttaccctgg cgggagccga tcacgccatt attccggtca
     8281 tgcttggtga tgcggtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt
     8341 acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggcgcgtatt cgtacccaga
     8401 tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg
     8461 gtaaacaact gggcgttatt gcctgaggat gtgagatgaa agcgttatcc aaactgaaag
     8521 cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc
     8581 tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg
     8641 agtggtcgca aaaaaccatc ccggtgccga tggtcgtggg ccatgaatat gtcggtgaag
     8701 tggtaggtat tggtcaggaa gtgaagggct tcaagattgg cgatcgcgtt tctggcgaag
     8761 gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca
     8821 acacgatcgg cgtcggcgtt aaccgcccgg gctgcttcgc cgaatatctg gtgattccgg
     8881 cgttcaacgc cttcaaaatt cccgacaata tctccgacga cctggcttcc atttttgatc
     8941 ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggttggcgag gatgtgctgg
     9001 tctctggtgc aggcccgatt ggtattatgg cagcggcggt ggcgaaacac gttggtgcac
     9061 gcaatgtggt gatcactgat gttaacgaat atcgtctcga actggcgcgc aaaatgggta
     9121 tcacccgtgc ggtcaacgtc gctaaagaaa accttaatga tgtgatggca gaactgggca
     9181 tgaccgaagg ctttgatgtc ggtctggaaa tgtccggtgc gccgccagcg tttcgtacca
     9241 tgctcgacac catgaaccac ggcggtcgca ttgcgatgct gggtattcca ccgtccgata
     9301 tgtctatcga ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc
     9361 gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt ctggatctct
     9421 cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc
     9481 gttcgggcca gtccgggaaa gtaattctga gctgggatta acacgaacaa gggctggtat
     9541 tccagccctt ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa
     9601 gatatcgtaa ttgcgctgat ctcttatata gctgctctca ttatctctct accctgaagt
     9661 gactctctca cctgtaaaaa taatatctca caggcttaat agtttcttaa tacaaagcct
     9721 gtaaaacgtc aggataactt cagaggtcgt cggtaattta tgatgaacag caccaataaa
     9781 cttagtgtta ttattccgtt atataatgcg ggcgatgatt tccgcacttg tatggaatct
     9841 ttaattacgc aaacctggac tgctctggaa atcattatta ttaacgatgg ttcaacggat
     9901 aattctgttg aaatagcaaa gcattacgca gaaaactatc cgcacgttcg tttgttgcat
     9961 caggcgaatg ctggcgcatc ggtggcgcgt aatcgtggga ttgaagtggc aacgggcaaa
    10021 tatgtcgctt ttgtcgatgc tgacgatgaa gtctatccca ccatgtacga aacgctgatg
    10081 accatggcgt tagaggacga cctcgacgtg gcgcagtgca acgctgactg gtgttttcgt
    10141 gaaacgggag aaacctggaa atccatcccc agcgatcgcc ttcgctcaac cggcgtatta
    10201 accggcccgg actggctgcg gatggggctt tcttcgcgcc gttggactca cgttgtctgg
    10261 atgggggttt atcgccgcga tgttattgtt aaaaataaca ttaaatttat tgccggatta
    10321 catcatcagg atattgtctg gacaacagaa tttatgttta atgcgctgcg tgcgcgatat
    10381 accgagcaat cgttatataa atattatctg cataatacgt cagtgagtcg gttgcataga
    10441 caagggaaca aaaaccttaa ttatcaacgt cactatatta agattactcg tctgctagag
    10501 aaattaaatc gaaattatgc cgacaaaatt acgatttatc cggaatttca tcagcaaata
    10561 acttacgaag cattgcgtgt ttgccatgcg gtgcgcaaag agccggatat tattacccgc
    10621 caacggatga ttgccgagat atttacttcc ggtatgtggt aatgactcca acttattgat
    10681 agtgttttat gttcagataa tgcccgatga c
//
LOCUS       DAZRVY010000133        10709 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZRVY010000133 DAZRVY010000000
VERSION     DAZRVY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.133"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..705
                     /locus_tag="ACHXOX_003483"
     CDS             <1..705
                     /locus_tag="ACHXOX_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006862.1"
                     /translation="ITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIFNI
                     NALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSES
                     IKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFA
                     CVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKR
                     ENMTPFMYVLNTPPVI"
     gene            715..1194
                     /gene="ykfM"
                     /locus_tag="ACHXOX_003484"
     CDS             715..1194
                     /gene="ykfM"
                     /locus_tag="ACHXOX_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR7006863.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1331..2116)
                     /locus_tag="ACHXOX_003485"
     CDS             complement(1331..2116)
                     /locus_tag="ACHXOX_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006864.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(2446..2522)
                     /locus_tag="ACHXOX_003486"
     tRNA            complement(2446..2522)
                     /locus_tag="ACHXOX_003486"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2486..2488),aa:Asp,seq:gtc)
     gene            complement(2655..3386)
                     /gene="dnaQ"
                     /locus_tag="ACHXOX_003487"
     CDS             complement(2655..3386)
                     /gene="dnaQ"
                     /locus_tag="ACHXOX_003487"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR7006865.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            3451..3918
                     /gene="rnhA"
                     /locus_tag="ACHXOX_003488"
     CDS             3451..3918
                     /gene="rnhA"
                     /locus_tag="ACHXOX_003488"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR7006866.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(3915..4637)
                     /locus_tag="ACHXOX_003489"
     CDS             complement(3915..4637)
                     /locus_tag="ACHXOX_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7006867.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            4671..5426
                     /gene="gloB"
                     /locus_tag="ACHXOX_003490"
     CDS             4671..5426
                     /gene="gloB"
                     /locus_tag="ACHXOX_003490"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR7006868.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5498..6856
                     /gene="mltD"
                     /locus_tag="ACHXOX_003491"
     CDS             5498..6856
                     /gene="mltD"
                     /locus_tag="ACHXOX_003491"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR7006869.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(6905..7528)
                     /locus_tag="ACHXOX_003492"
     CDS             complement(6905..7528)
                     /locus_tag="ACHXOX_003492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7006870.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(7532..8332)
                     /locus_tag="ACHXOX_003493"
     CDS             complement(7532..8332)
                     /locus_tag="ACHXOX_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR7006871.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            8573..9487
                     /gene="yafC"
                     /locus_tag="ACHXOX_003494"
     CDS             8573..9487
                     /gene="yafC"
                     /locus_tag="ACHXOX_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR7006872.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(9484..10287)
                     /gene="dkgB"
                     /locus_tag="ACHXOX_003495"
     CDS             complement(9484..10287)
                     /gene="dkgB"
                     /locus_tag="ACHXOX_003495"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR7006873.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(10450..10526)
                     /locus_tag="ACHXOX_003496"
     tRNA            complement(10450..10526)
                     /locus_tag="ACHXOX_003496"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10490..10492),aa:Asp,seq:gtc)
BASE COUNT         2951 a         2452 c         2429 g         2877 t
ORIGIN      
        1 ataacatcat ttgattacat tcattttatc actacacatc cttcaggtat aaaagataca
       61 ctcgcctggt tagttaacgc gagtaaatta atgactgagt ttgatgacaa tgggaaaatt
      121 atatttaaca ttaatgcctt aaagtataca aaagcaagtt actttgaaat acttggagag
      181 aaatatataa aaatcacaac atccagcccc tggcttttag aaaagctcgg taaatatatt
      241 ttctcttcac gagcgcctca agttctggaa ctcgctattg gctggcgagg agctttatct
      301 gaatcaataa aaggagttaa gttctgtatc tggttttcag tagcctggag aaccatagaa
      361 tttattatga gttcagagcg agatctggtt aattttcttg gcgatttttc aatggatgtg
      421 gccaaagcag ttatagccgg tggtgttgca accgctattg gaagcctggc ttcttttgcc
      481 tgtgttagct ttggctttcc agtaattctt gtcggaggag caattttact gacagggata
      541 gtgtgtacag ttgttttaaa tgaaatcgat gctcaatgcc atttatcaga aaaattaaaa
      601 tatgcaatta gagatggact aaaacggcaa caggaacttg ataaatggaa aagggaaaac
      661 atgactccat ttatgtatgt tcttaacact ccacccgtga tatgaggatt aaccatgcgt
      721 ttacatgtta aattaaaaga gtttctttcg atgtttttta tggccatatt atttttccct
      781 gcattcaatg cttctttgtt ttttaccgga gttaaacctc tatattcaat aataaaatgc
      841 tccacggaaa tattttatga ttggagaatg ttgattctgt gctttggttt catgtcgttt
      901 tcctttttaa atattcatgt aattttactt acgataataa aattattctt aatcaaaaaa
      961 acgaaggtag tcaatttcgc cacagacata acaatacagc taactcttat atttttacta
     1021 atagctatcg taatagcacc tttaatagct ccttttgtca caggatatgt caatgctaat
     1081 tatcacccct gcggtaataa tacgggaatt ttcccaggag caatctatat taaaaatggc
     1141 atgaaatgta ataatgaata tatttctcgc aaggaagatt cagcagttaa ataattaaac
     1201 attctaaaac aaacagtttt taactccgga ttccatgtcc tttcttgcac tgtctttgtc
     1261 aagaaaaatt tcttatgcaa agaattttgt attatttccg ccagaaattc ctggcggcag
     1321 atataattta ttattgatag cggttaacca aatcttttac catcgcttta taggtctttt
     1381 gcttaagttg catgatttgc tgctctgtca tatcagtcgt actggcttca ttcttgtcgg
     1441 gcaatggtgg aagcacttta tattcataat tcaccggcga ccaggcagcc cactcacctg
     1501 tagctacatc caccagcgtg aatcgtacca gatagcgcag tgacatcgtg tcagtcaatt
     1561 tttgattttt ataatctgac caaactattg atttcaatcc agagtcatat tttcctgtct
     1621 gcaacgtatc ctggaaaaca ataatcgctt tctgatgccc ttttgcagcc acaaaacgca
     1681 gtgcctgcat ccagttcata ttctcagcac tggcaacatc ctcgttctca tttttatctt
     1741 tgtttttgtt acaagtcaga ggcttttgcc tgtccgggat accagagaat gtggaaacag
     1801 tataatattt tcgcatctcc tcctgcataa tggtttccgg tgcgcggttg ccggattgaa
     1861 caagtattac aggagaatta aggggaacgc ggaactcatc tccatcaagc gcagcttgaa
     1921 tatcctcttc ggatacggcc agagtggttt cattaccgaa taaatctttg tctgtcagtt
     1981 gctggggagt gtttttatta atattacttt gtgtgacttg agcttttttc ttcttttcat
     2041 caatagaaga ttcagaggca catcctgcaa gcagcgagaa taatacacat atacaacaaa
     2101 cccttcttaa attcataatc ggacactccc tcgcctgtca taatcatggt aatgtttagc
     2161 taacgatttt taattatata ctccacttga atcagaacaa acaaaaataa cagtatatat
     2221 aaattcatta aatcaataga aaaatgaaaa tcataacatt caaatatatt taaattgaat
     2281 gattcgatag tatgattaaa tattaatgat atggtctacc atatgtgatt aacacattta
     2341 cagcataaca aaaccataga ccgaagaaat agcgggattt aaatgcaaaa aaaccgcaaa
     2401 attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg gaacggacgg
     2461 gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
     2521 ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc cttcgtcaac
     2581 ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct atttttagcg
     2641 ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc caccagatca
     2701 agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg taacttactt
     2761 gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt ctctccttcc
     2821 atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc cgcaaggatc
     2881 tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc gtagcgggca
     2941 cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac cgcaaggcta
     3001 tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa cgaaaactcg
     3061 tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc gccgcggata
     3121 tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa aaattcatcg
     3181 gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt gagataaaca
     3241 tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat ctcaatgatc
     3301 ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt atcgagaacg
     3361 atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttat cgttttacag
     3421 ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat tttcaccgat
     3481 ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg ctattttacg ctatcgcgga
     3541 cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat ggagttgatg
     3601 gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt gagtaccgac
     3661 agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa acgtggctgg
     3721 aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct tgatgctgcc
     3781 ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc atgccggaca cccggaaaac
     3841 gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc ccacactgga agatacgggc
     3901 taccaagtcg aagtttaagc ttgtggttta cgacattgcc gggtggctcc aaccgcctgg
     3961 cgaattcgtg gcttgttttt actctgtttc atcgggttta gcgttaaagg aatagtccgt
     4021 ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt caatagtttt
     4081 cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac ttcaaaattc
     4141 aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt atagggcgag
     4201 gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg attgaagcca
     4261 ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa taaacgatgc
     4321 ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga tttatcggca
     4381 aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc agaaacattc
     4441 acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt gccaatctta
     4501 agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc cagcgccttg
     4561 cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac agtttgaggg
     4621 actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct atgaatctta
     4681 acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa gcaggtcgct
     4741 gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc gccaataact
     4801 ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc ggcgtaaaag
     4861 aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca caagataagg
     4921 gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat gaatttagtg
     4981 taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa ccttatctat
     5041 tttgcggcga cacgctgttt tctggtgggt gtggtaggtt gtttgaaggg acagcatcac
     5101 aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg gtatgttgtg
     5161 ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg cacgatttgt
     5221 ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa ataacactac
     5281 ccgtaattct gaaaaatgag cggcaaatta atgttttttt aagaacggaa gatattgatt
     5341 taattaatgt aattaatgaa gaaacattat tgcaacaacc tgaagagcgt tttgcatggt
     5401 taaggtcaaa gaaagatagg ttctgataaa actttcttgt catcggctcc gttcgccgtt
     5461 atgatcggtc gtcttttaag caactattga cacacacatg aaggcaaaag cgatattact
     5521 cgcctctgtc ctgctcgtgg gttgccagag taccggcaac gttcaacagc acgcacagag
     5581 cctttctgca gctggtcaag gggaagcagc aaagtttaca agtcaggcac gatggatgga
     5641 cgatgggacg tctatcgcgc cagatggtga cttgtgggct ttcattggcg acgagctaaa
     5701 gatgggaatt ccggaaaatg accggattcg cgaacagaaa cagaaatatt tacgcaataa
     5761 gagctatctc cacgatgtaa ctttacgggc agagccgtat atgtactgga tagcagggca
     5821 agttaaaaaa cgtaacatgc ctatggaact ggtactacta cccatagtgg agagcgcttt
     5881 tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc tggcagatca ttccgagcac
     5941 ggggcgcaat tatggtttga aacagacccg caattatgac gcgcgtcgcg atgttgttgc
     6001 ttcaacaact gccgcgctga atatgatgca gcgtctgaac aaaatgtttg acggcgactg
     6061 gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg caattaaaac
     6121 gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta ccgttgccgc aggaaacgaa
     6181 gcagtacgtg cctaaaatgc tggcattgag tgatattctc aaaaacagca agcgttatgg
     6241 cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc tgagtagccc
     6301 ggttgaaatg gcgaaggttg cagatatggc ggggatttcc gtcagcaagc tgaagacatt
     6361 caacgctggc gtgaaaggct ccacgctggg cgcaagtggg ccgcagtacg tgatggtgcc
     6421 aaagaagcat gcagatcaac tgcgtgaatc tctcgcttca ggcgaaattg ctgctgtaca
     6481 gtcgacgctg gttgccgaca atacgccgct taacagccgt gtttacaccg tacgctctgg
     6541 cgacacgctt tcaagcatcg cttcacgtct cggcgtaagc accaaagatt tgcagcagtg
     6601 gaacaaactg cgcggatcta agctgaagcc aggccaaagt ttgacgattg gtgcaggcag
     6661 tagcgcacag cggttggcaa acaacagcga tagcattacg tatcgtgtgc gcaaaggcga
     6721 ttcgctttca agcattgcta aacgccacgg cgtgaacatc aaagatgtga tgcgctggaa
     6781 cagcgacact gcgaatctgc aaccaggcga taagctgacg ttgtttgtga aaaacaacaa
     6841 catgccagat tcctgacaaa ccagataata aaaaaggcac cgattccccc ggtgcctttt
     6901 ttatttatgc cgctttatgt gcttctacca tgatgatatc actggtgaaa gagccatcag
     6961 tctgcaagga aaaatacgtt ctcacctctg tcgatgcgct ctgttggtaa atgcgaatag
     7021 cgtctactaa cgcttctggc gtacgcattc tcacgaccca tgaagaaaat tccagcggta
     7081 acttatctgt aattaaatta tcaactatca gattggcttc attgattaac gtcaaccacg
     7141 caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa tgcttctacc gcctgtaacc
     7201 agatgtcgcg cactgggtga cccggagaca ttacatccat cacaatcagc ctaccgccag
     7261 gtttcaatat cctattcact tctcgcagtg ctgcaccaac atcatgccag tgatgggcag
     7321 aataacggct gataacaata tcaaatgcgt tatcggcaaa tggcagactt gcggcatatc
     7381 cctggcgggt ggtgatattt ttcagttgcc gggcttcggc agcttgtgcc acgacatcca
     7441 gcatgtgggc agataagtca tacgccacca ccgcgctcac gttttgcgca gcgccaaacg
     7501 ttggtctgcc ctgcggcaga cctgacatac cttatttatc aggcttgccg ggactgaatt
     7561 caacgagtag cggattgtga tcggaagcgc gcgtaaccag tacagaagct tcactgacgt
     7621 tcagaccacg gtagaaaaca aaatcgagcg ggcgaccaaa cgcccgacgg cgctgatcat
     7681 cggtaaaacg cacctggcgc agcgacattt cccgcgcaaa gcgatataac gcgttcatcc
     7741 ttctacggct ccaggcattg aaatctcccg ccataatgac cgggccgctg tggtgagcta
     7801 tctgatcacc aataggaagt aactgcttac tatagacatc cacgcccaga ctgaagttga
     7861 cggcgtgtat attaaccacc atcaacaggc gggtgtcagg taatggatag accgtcacca
     7921 gtgccgactt cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg
     7981 gatgtgccgc cgaaagggtc attacgccag aaggatgttg tggcagcacg aaagcgggta
     8041 cctgatcggc ggcaagatag ttagcggtcg caaactgtac caactctggc gttgtttgcg
     8101 cttcctgcaa taacaccaga tgtgcgtctt tgccgtaatt ctttaatacc gacaaccatt
     8161 cagcgcgttg ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta
     8221 acggttctcc gggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc
     8281 tctccgcagg ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact
     8341 ttgaaacctc tattacgtca acccttccgc actcgggagg gttctactgt tatagggatt
     8401 ttagctcact ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacaccggt
     8461 cagaaatttg ctcattattg agaacattaa atcggatgac atatcacgca tcctgatggt
     8521 ttaatgacaa ataaattcaa aatatctcat ctattttgca acaggagcaa aaatgaaagc
     8581 cacgtcggaa gaactcgcca tttttgtttc ggtcgtcgaa agcggcagct ttagccgggc
     8641 agcggaacaa ttagggcaag caaactcagc ggtaagccgg gcggtgaaaa agctggaaat
     8701 gaaacttggc gttagcctgc ttaatcggac cacgcggcaa cttagcctga cggaagaagg
     8761 cgagcgttat tttcgtcgcg tacagtcaat tttgcaggag atggcagcgg cagaatcaga
     8821 aattatggag acgcgtaata caccgcgtgg actgttacgg atcgatgccg caactccagt
     8881 ggtgctgcac tttctgatgc cgttaattaa gcctttccgt gaacgctatc cggaagtcac
     8941 tttatcgcta gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc
     9001 gatacgcgct ggtacgttaa cggactccag cttacgtgcc aggccgttat ttaacagtta
     9061 tcgaaaaatt atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga
     9121 cgatttaaag caacatgttt gcctgggatt cactgaaccc gcttccctca atacctggcc
     9181 gatagcctgt agtgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg
     9241 ggaaacactg aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactacat
     9301 gatcgacaaa gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc
     9361 agtggaaatg ccattcagtg ccgtctatta cagcgaccgt gcggtaagta cgcgcatccg
     9421 ggcttttatc gatttcctta gcgagcatgt aaaaacagct cccggaggag ctgtcagaga
     9481 ggcttaatcc cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag
     9541 tgcggcgatc gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact
     9601 ttccaggttt ttacgtttag tagaagaagg aattactgag taaccttccc ccatagccca
     9661 cgccagaatc acttgtgccg gagtcgcatt gtgtttagct gcaatacgag caataacctc
     9721 atctttcagt gccttaccat aagccagcgt catataggaa gtgatatgaa taccgtgctg
     9781 tttagcccag gcaaccactt tacggttttg cagataagga gagagttcaa tctggttggt
     9841 agcgatgttt tcagcgccaa cagcagcaat cgctttttcc atcaacggga tcgtgaagtt
     9901 ggaaataccg atctcacgtg tcagaccttg ttttttggcc tccagcagcg cctgcataaa
     9961 ctcttcaaca gagacttcat cgtttggtga cggccagtgg attagcgtca gatcaacata
    10021 atcggtacgc aatttttgca ggctctcttt tagactcggg atcaatttgt ctttgctgag
    10081 attttcaatc cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc
    10141 aatcgcctga cctactgcgg cttcgttatc atagatttgt gcagtatcaa ttgcgcgata
    10201 accaagttca agcgccgttt tcacagatga aataacaacg tcgtctttga gacggaaagt
    10261 acctaaacca aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact
    10321 gaggtgatta tgggcttcag actgttgcgg aaaaagagca taaaatgcag aggatttttg
    10381 cgattctggc aataatagat atacagacga aaaaaaacct caagcgttaa ctcaaggctt
    10441 cttaataagt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt
    10501 attctaaccg actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc
    10561 ggcttactgc ttaatttgat gcctggcagt ttatgaatca cttcgtgatt cacccttcgg
    10621 gccgttgctt cgcaacgttc aaaagctttc gcttttgtcc tactctctta tcgagtagtg
    10681 aactgcctca aaggtcagtg ccattcttc
//
LOCUS       DAZRVY010000134        10686 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZRVY010000134 DAZRVY010000000
VERSION     DAZRVY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.134"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHXOX_003497"
     CDS             complement(<1..784)
                     /locus_tag="ACHXOX_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR7006874.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHXOX_003498"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHXOX_003498"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR7006875.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHXOX_003499"
     CDS             1860..2747
                     /locus_tag="ACHXOX_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR7006876.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHXOX_003500"
     CDS             2780..3790
                     /locus_tag="ACHXOX_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR7006877.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHXOX_003501"
     CDS             3860..5293
                     /locus_tag="ACHXOX_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7006878.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHXOX_003502"
     CDS             5392..6741
                     /locus_tag="ACHXOX_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7006879.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHXOX_003503"
     CDS             6731..7618
                     /locus_tag="ACHXOX_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR7006880.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7758..8357
                     /gene="yihX"
                     /locus_tag="ACHXOX_003504"
     CDS             7758..8357
                     /gene="yihX"
                     /locus_tag="ACHXOX_003504"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR7006881.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8351..9223
                     /locus_tag="ACHXOX_003505"
     CDS             8351..9223
                     /locus_tag="ACHXOX_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR7006882.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9220..9657
                     /gene="dtd"
                     /locus_tag="ACHXOX_003506"
     CDS             9220..9657
                     /gene="dtd"
                     /locus_tag="ACHXOX_003506"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR7006883.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9702..10643
                     /gene="fabY"
                     /locus_tag="ACHXOX_003507"
                     /gene_synonym="yiiD"
     CDS             9702..10643
                     /gene="fabY"
                     /locus_tag="ACHXOX_003507"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR7006884.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2531 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaatg ctctatatct ttgatttagg taatgtgatt gtcgatatcg
     7801 actttaaccg tgtgctggga gcctggagcg atttaacgcg tattccgctg gcatcgctta
     7861 agaagagttt tcatatgggg gaggcgtttc atcagcatga gcgtggggaa attagcgacg
     7921 aagcgttcgc agaggcgctg tgtcatgaga tggctctacc gctaagctac gagcagttct
     7981 ctcacggctg gcaggcggtg tttgttgcgc tgcgcccgga agtgatcgcc atcatgcata
     8041 aactgcgtga gcaggggcat cgcgtggtgg tgctttccaa taccaaccgc ctgcatacca
     8101 ccttctggcc ggaagaatac ccggaaattc gtgatgctgc tgaccatatc tatctgtcgc
     8161 aagatctggg gatgcgcaaa cctgaagcac gaatttacca gcatgttttg caggcggaag
     8221 gtttttcacc cagcgatacg gtctttttcg acgataacgc cgataatata gaaggagcca
     8281 atcagctggg cattaccagc attctggtga aagataaaac caccatcccg gactatttcg
     8341 cgaaggtgtt atgctaaaaa ccattcagga caaagccagg caccgtaccc gtccactatg
     8401 ggcctggcta aaactactct ggcaacgtat tgatgaggac aacatgacaa ccctggcagg
     8461 taaccttgcc tatgtgtctt tgctctcatt agtgccgctg gttgccgttg tttttgcgct
     8521 tttcgccgct tttcccatgt tttccgacgt cagcattcag ttacgtcact ttatttttgc
     8581 caactttctg cctgctactg gcgatgttat tcagcggtat atcgaacaat ttgttgccaa
     8641 ttccaacaag atgaccgccg ttggggcgtg cgggctgatc gtcaccgcgt tattgttgat
     8701 gtactccatc gatagcgcgt tgaataccat ctggcgcagt aaacgagcgc gaccaaaaat
     8761 ttactcgttt gccgtgtact ggatgatctt aacgctgggg ccgctgctgg caggggccag
     8821 tctggcgatc agttcctatt tgttctccct gcgctgggcg agcgatctca atactgtcat
     8881 cgataacgtg ctgcgtattt ttccgctgct gttgtcgtgg atctccttct ggttgctgta
     8941 cagcattgtt cctaccatcc gcgtacctaa ccgcgacgcg attgtcggcg cgtttgtcgc
     9001 tgcactcctg ttcgaagcag gaaagaaagg tttcgcgctt tatatcacca tgttcccgtc
     9061 atatcagctc atttacggcg tgctggcggt gatccccatt ctcttcgttt gggtctactg
     9121 gacatggtgt atcgtcttgc ttggcgcgga aattactgtc actctcgggg aataccgcaa
     9181 actaaaacaa gcagctgaac aagaagaaga cgacgaacca tgattgcatt aattcaacgc
     9241 gtaacccgtg ccagcgtcac cgtggaggga gaagtgacgg gcgaaattgg cgcgggactt
     9301 ttggtgttat tgggtgtcga aaaggatgac gacgaacaga aagcaaaccg tctgtgcgag
     9361 cgtgtgctcg gctaccgcat ctttagcgat gccgaaggca agatgaatct caacgtgcaa
     9421 caggcgggcg gcagtgtact ggtggtttcc cagtttaccc tcgccgcaga taccgaacgg
     9481 gggatgcgcc caagtttctc caaaggcgca tcaccggatc gcgcagaggc gttatatgac
     9541 tatttcgtcg aacgctgccg ccagcaagag atgaacacgc aaacaggacg cttcgctgcc
     9601 gatatgcagg tatcgctggt caatgatggc cccgtgacat tctggttgca ggtatgagcc
     9661 tgcttccagg gttgtcacgg gaaacaagag agagtatcgc tatgtatcac cttcgggttc
     9721 cacaaacaga agaagagtta gagcgttact atcagtttcg ctgggaaatg ttgcgtaagc
     9781 ccctgcatca accaaaaggt tcggaacgcg acgcgtggga tgcgatggcg catcaccaga
     9841 tggtcgtcga cgagcagggt aatctggtgg cggtaggccg attgtatatt aatgccgaca
     9901 atgaagcgtc cattcgcttt atggccgttc acccggacgt gcaggacaaa gggttaggaa
     9961 cgctgatggc gatgaccctg gagtcggtgg cgcgtcagga aggcgttaag cgcgtgacct
    10021 gtagcgcccg tgaagacgcg gtggagtttt tcgccaagct gggattcatt aatcaggggg
    10081 agatcaccac cccaactacc acgccgattc gccatttttt gatgattaaa cccgttgcca
    10141 ctctggatga cattctgcat cgcggtgact ggtgcgcgca gctgcaacag gcgtggtacg
    10201 aacatatccc gttaagcgaa aaaatgggcg tgcgcattca gcaatatacc gggcaaaaat
    10261 ttatcaccac catgccagaa accggtaatc agaatccgca ccatacgttg tttgccggga
    10321 gtttattctc gctggcgacg ctcaccggtt ggggacttat ctggctgatg ctgcgtgaac
    10381 gccacctcgg cggaacgatt attctggcgg atgcgcatat ccgctacagc aagccgatta
    10441 gcggtaaacc tcatgcggta gccgaccttg gtgccttaag cggcgatctc gaccgtctgg
    10501 cgcgcggacg aaaagcacgg gtgcagatgc aagttgaaat ctttggcgac gagacgccgg
    10561 gtgcagtgtt tgaaggcacg tatatcgttc tgcccgcgaa gccatttggc ccgtatgaag
    10621 agggcgggaa cgaagaagag tagcttatgg agaggggcgg tgctgcctct ctcattcatt
    10681 ccggtc
//
LOCUS       DAZRVY010000135        10505 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZRVY010000135 DAZRVY010000000
VERSION     DAZRVY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.135"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            183..758
                     /gene="ssuE"
                     /locus_tag="ACHXOX_003508"
     CDS             183..758
                     /gene="ssuE"
                     /locus_tag="ACHXOX_003508"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR7006885.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            751..1710
                     /gene="ssuA"
                     /locus_tag="ACHXOX_003509"
     CDS             751..1710
                     /gene="ssuA"
                     /locus_tag="ACHXOX_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR7006886.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1707..2854
                     /gene="ssuD"
                     /locus_tag="ACHXOX_003510"
                     /pseudo
     CDS             1707..2854
                     /gene="ssuD"
                     /locus_tag="ACHXOX_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2866..3657
                     /gene="ssuC"
                     /locus_tag="ACHXOX_003511"
     CDS             2866..3657
                     /gene="ssuC"
                     /locus_tag="ACHXOX_003511"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR7006887.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3654..4421
                     /gene="ssuB"
                     /locus_tag="ACHXOX_003512"
     CDS             3654..4421
                     /gene="ssuB"
                     /locus_tag="ACHXOX_003512"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR7006888.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4628..7240)
                     /gene="pepN"
                     /locus_tag="ACHXOX_003513"
     CDS             complement(4628..7240)
                     /gene="pepN"
                     /locus_tag="ACHXOX_003513"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR7006889.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7506..8708
                     /gene="pncB"
                     /locus_tag="ACHXOX_003514"
     CDS             7506..8708
                     /gene="pncB"
                     /locus_tag="ACHXOX_003514"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR7006890.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8878..10278
                     /gene="asnS"
                     /locus_tag="ACHXOX_003515"
     CDS             8878..10278
                     /gene="asnS"
                     /locus_tag="ACHXOX_003515"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR7006891.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2375 a         2702 c         2826 g         2602 t
ORIGIN      
        1 gattcaacat ctaatgacta tccaacaaat aaatatgcgt gatatataaa gccctttctt
       61 tagtttattt tcagaaaaag atacacaaaa tttatatttg gcattttctt gtctctccga
      121 cagactgacc gaattgttat caatgttaac aaaaaaagaa caattggtta taaggagaga
      181 gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc agctccttgc
      241 tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga ggtttatcac tggaatctgc
      301 aaaacttcgc cccggaagat ctgctttacg ctcgtttcga tagtccggca ctcaagacct
      361 tcaccgaaca gctgcaacag gctgatggac tgatagtcgc tacacctgtg tataaagccg
      421 cctattccgg cgcgctgaaa accctgctcg acctgctgcc agagcgcgct ttgcaaggca
      481 aagtggtgct accgctggcg acgggcggta cggtggctca tctgctggcg gtcgattatg
      541 cccttaagcc agttttaagt gcgatgaaag cccaggagct cctgcacggc gtgtttgccg
      601 atgactcaca agttattgat taccatcaca aaccccagtt cacgccaaat ctgcaaaccc
      661 gtcttgatac cgcgctagaa actttctggc aggcattgca ccgccgcgat gttcaggttc
      721 ctgaccttct gtctctgcga ggtaatgtcc atgcgtaaca tcattaaact ggcgctggcg
      781 ggattgctta gcgtctctac ttttgcggtt gctgcagaat cctcgcctga agcgttacgt
      841 ataggctatc agaaaggcag tattggtatg gtattggcaa aaagccacca gttactggaa
      901 aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc ccgcgggtcc gcaaatgttg
      961 gaagcgttaa acgttggcag tattgatctc ggcagtaccg gggatattcc gccaatcttt
     1021 gcccaggctg ccggggctga tttgatatat gtgggcgtcg agccaccgaa gcccaaagcc
     1081 gaagtgattc tggtggcaga aaacagcccg atcaaaaccg tagccgatct taaaggtcac
     1141 aaagttgcct ttcagaaagg ttccagttca cacaaccttt tactgcgtgc actgcgtcag
     1201 gccggactta agtttactga catccagccc acttatctga cgccagctga tgcccgcgcc
     1261 gcgttccagc aaggtaacgt tgacgcctgg gctatctggg atccctacta ctccgctgcg
     1321 ttattacagg gcggcgtgcg ggtgctgaaa gacggcaccg atctcaatca aactggatcg
     1381 ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg cttttattca gggcgtactg
     1441 gcaaccttta gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag catcgcttta
     1501 ctggcaaaaa cgatgggctt accggcaccg gtgattgcct cgtatctgga tcatcgtcct
     1561 cccaccacca tcaaaccggt gagcgctgaa gttgccgcct tacagcagca aacggcagat
     1621 ctgttttatg aaaaccgtct ggtgtcgaaa aaagttgata ttcgccagcg catctggcag
     1681 cccactcacc tggaaggaaa acaattatga gtctgaatat gttctggttt ttaccgaccc
     1741 atggtgacgg gcattatctt ggaacggaag aaggttcacg cccggttgat cacggctatc
     1801 tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac cggtgtgctg atcccaacgg
     1861 ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat gatcccggtg acgcagcggc
     1921 tgaagtttct tgtcgccctg cgtcccagcg taacctcacc taccgttgcc gctcgccagg
     1981 ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt taatctggtc acaggcagcg
     2041 atccacaaga gttggcaggc gacggcgtgt tccttgatca tagcgagcgc tacgaagcct
     2101 cggcggaatt tacccaggtc tggcggggcg cttattgctg ggggaaagcg tcgatttcaa
     2161 cggtaaacat attcatgtgc ggggagcaaa actgctcttc ccgccgattc aacagccgta
     2221 tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg cagaacaagt
     2281 tgatctctac ctcacctggg gcgaaccgcc agagctggta aaagagaaga tcgaacaagt
     2341 gcgggcgaaa gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat
     2401 tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga
     2461 tgatgaaact atcgccaaag cacaggccgc attcgcccgg acggattccg taggacaaca
     2521 gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg
     2581 ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg atggtcctac
     2641 ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg tgctttcggg
     2701 ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac atctggatgt
     2761 cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggcgaa
     2821 tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc aacgccagtg
     2881 aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg
     2941 caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc ggaaggagtg
     3001 gtgacggcgt tctggacgct ctccgccagc ggcgaattgt ggcagcatct ggcgatcagt
     3061 tcctggcggg cgctgattgg cttttcaatt ggcggatcgc tgggcttgat tctgggactg
     3121 attagcgggc tgtcacgctg gggagaacgg ctgctggata cctcaattca gatgttgcgc
     3181 aacgtaccgc atctggcgct gatcccgctg gtgattttgt ggtttggcat tgatgaatcc
     3241 gcaaaaatct ttctggtggc gctcggcacg ctattcccca tttatatcaa cacctggcat
     3301 ggcatccgta atatcgatcg cggtctggtg gaaatggcgc gtagctatgg attatccggc
     3361 ataccgctgt ttatccatgt gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg
     3421 cgttttgcgt tgggcctgat gtggctgacg ctgattgttg ccgaaaccat ttctgccaat
     3481 tcaggcattg gttatctggc gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta
     3541 gtcgctatta ttctttacgc cctgctcggc aaacttgctg acgtcagcgc gcagctgtta
     3601 gagcgcctct ggttgcgctg gaacccggct tatcatttga aggaggccac ggtatgaata
     3661 ctgctcgtct gaaccagggc acaccattgt tgctcaatgc agtaagcaaa cattacgcgg
     3721 aaaatatcgt cctgaaccaa ctggatttac atattccggc aggtcagttt gtggcggtgg
     3781 tgggccgcag cggtggtggc aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc
     3841 caaccgcagg cgatgtgtta gcgggcacca caccgctggc tgagattcag gaagacacgc
     3901 gaatgatgtt tcaggatgcg cgtctgctgc cgtggaaatc ggtgattgat aacgttgggt
     3961 taggccttaa aggccagtgg cgcgatgccg cgcgtcgggc gttggctgca gtaggactgg
     4021 agaatcgcgc cggggaatgg cctgccgcac tttctggcgg gcagaaacag cgtgtggcgc
     4081 tggcaagagc gttgattcat cgaccgggtt tattgttgct tgatgaaccg ctcggggcac
     4141 tggacgcctt aacgcgactc gagatgcagg atttgattgt gtcactttgg cagcagcacg
     4201 gctttaccgt actgttggtg acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg
     4261 tgttgttaat tgaagaggga aaaattggtc tggatttgac agtggatatc ccccgtccgc
     4321 gtcgattagg gtcggtgcga ttagcggagc tggaagcgga agtattgcaa cgggtaatgc
     4381 ggcgtgggca ttctgaacaa ctgattcggc gtcatggtta atatgccgga tgtggcgcac
     4441 aaaaccagca ttttcaacgg acttgtaggc ctgataagac acgtcaagca tcgcatcagg
     4501 cagttgggca cggctgccgg atgcggcgtg aacgccttat ccagcctaca aaaccagcaa
     4561 tttcaacgga gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc attttggctc
     4621 aaaagtatca agccagtgct ttggtgatct tctcgtacag atcgccagag agattttcca
     4681 gccctttcaa ctgttccagc gccgcgcgca ttttctcctg acgtttggca tcataacgtt
     4741 tcaggcgaat cagcggttca atcagacgtg aagctacctg cgggttacgg ctgttgaggt
     4801 cggtaagcat ttccaccagg aactggtaac cgctgccatc ttcggcatgg aacgctgccg
     4861 ggttgctgcc cgcaaacgca ccaatcaacg aacgaatacg gttcgggttg ctcatggtaa
     4921 atgagcgatg ctgcaacaga ccacgcaccg tctccagcac attcgccgcc gggctggtgg
     4981 cttgcaggat aaaccattta tccatcacca gaccgtcctg atgccactta tcgtcgtact
     5041 cctgcatcag cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa agcgctgcca
     5101 gcgcatcggt catattgttt gcttcatggt attgcttgct caccagcacg tcagccagat
     5161 gcgtttcacc aaaagcaagg aagcgcaggc aggcatttcg cagagtgcgt tttgcaatat
     5221 cttcatgctc aacacggtac tcgctctggt aattcgcgtt gtaaatagcc agcagctcat
     5281 ccgccagttc agtcgccaga gtacgagtga gtgcttcgcg tacttcggca atagcaatcg
     5341 ggtcgatgat atcgaacagt tcagccattt cattgacaga aggcagcgtc aggatttctg
     5401 ccgccagcgc cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg tcagccacat
     5461 gtaccggcag agacagcggc tgcccttgct gatgacgggc gacgttcagc ttgatgtagg
     5521 ttgccagcaa actttgcgcc gcatcccaac gggagaaatc attacgcgca tgacgcatca
     5581 ggaaggtcag ttgctgatca ctccacttat attccagttt cactggcgca gagaattcgc
     5641 acagcagcgc aggcaccggc tggaagtaga cattatcaaa aacaaaggtc tgttccgcct
     5701 gggtgacgtt cagcacggaa ttcaccggat gaccgccttt ctgcaacggg atcactttgc
     5761 cttcgttgtc atacaattcg atggcaaacg gaatatgcag cggctgtttt tctgcctgat
     5821 ccggcgtggc tggcgtgcgc tggctgatgg tcagggtgta ctgctcagtt tccggattgt
     5881 agtcgtcttt gacggtcaca atcggcgtac cggactggct gtaccaacgg cggaaatggg
     5941 agagatcaac attcgacgca tcttccatcg cctgcacaaa gtcgtcacag gtcgctgcgc
     6001 taccatcatg acgctcgaaa tagagttgca tccctttctg gaagttttct tcgcccagca
     6061 gggtgtggat catacggatc acttccgcgc ccttctcgta aacggtcagg gtgtagaagt
     6121 tgttcatctc aatgaccatg tccgggcgga ttgggtgcgc catcgggctg gcgtcttccg
     6181 caaactgcaa tccgcgcatg gtgcgcacat tattgatgcg gttaactgcg cgggaaccaa
     6241 ggtcagagct gaattcctga tcgcggaaga cggttaaacc ttctttcagg ctgagctgga
     6301 accagtcgcg gcaggtcact cggttaccgg tccagttatg gaaatattca tggccgataa
     6361 cgcgttcaat atcgaggtaa tctttgtcgg tggcggtgtc ggtgcgggcc agcacatatt
     6421 tggagttaaa gatattcaga cccttattct ccattgcgcc catattgaag aaatccaccg
     6481 cgacgatcat atagatgtcg aggtcatact ccaggccgaa gcgttcttca tcccatttca
     6541 ttgagttttt cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt
     6601 acagctccag cgccacttcg cgaccagaac gcgtggtaaa ggtgtcgcgc agtacatcaa
     6661 agtcgcctgc caccagcgca aacaggtagc acggtttcgg gaacgggtcc tgccactgta
     6721 cccaatggcg tccgttttcc agttcacctt gcgcaacgcg gttaccgttg gaaagcagga
     6781 agggatattt ggttttatcg gcaataattt tggtggtaaa acgcgccagc acgtccgggc
     6841 ggtcgagata atacgtaata tggcggaaac cttcggcttc acactgggtg caaagcgcat
     6901 cgcctgactg ataaagccct tccagcgcgg tattcgccgc cgggcttatt tcattaacga
     6961 tcttaagcgt aaaatgctcc ggcaaattac tgatgaccag tgcgccctct tcttctttcc
     7021 aggcggtcca cggctcatca ttaatatgaa cagaaaccag tttgaggtct tcgccgttga
     7081 gacgaagtgg agcatctgat gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg
     7141 tcttttgcgc gtcgaggtca aaggtcaagt caatatcagt aatctggtaa tccggcgcac
     7201 gataatcgtg acggtatttg gcttgtggct gttgagtcat aaataacctt tagcatcttt
     7261 tatagagtct ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat
     7321 cttttcaggt acaaacgcct ttattgctac atttttataa catacaccgc gtaatgccat
     7381 cgaccagaaa ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa caaggtatac
     7441 tccagcagtt cctgaagatg tttattgtac taaacgctcc tgtacgagga cgctactgcg
     7501 cacctatgac acaattcgct tctcctgttc tgcactcgtt gctggataca gatgcttata
     7561 agttgcatat gcagcaagcc gtgtttcatc actattacga tgtgcatgtc gcggcggagt
     7621 ttcgttgccg aggtgacgat ctgctgggta tttatgccga tgctattcgt gaacagattc
     7681 aggcgatgca gcacctgcgc ctgcaggatg atgaatatca gtggctctct gccctgcctt
     7741 tctttaaggc cgactatctt aactggttac gcgagttccg ctttaacccg gaacaagtca
     7801 ccgtgtccaa cgataatggc aagctggata ttcgcttaag cggtccgtgg cgtgaagtca
     7861 tcctctggga agttcctttg ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac
     7921 cgcaggccga cgttgcgcaa gccctcgaca cgctggaaag caaattagtc gacttctcgg
     7981 cgttaaccgc cggtcttgat atgtcgcgct tccatctgat ggattttggc acccgtcgcc
     8041 gtttttctcg cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg
     8101 tgggcaccag caactacgat ctggcgcgtc ggctttccct cacgccgatg ggaacacagg
     8161 cacacgaatg gttccaggca catcagcaaa tcagcccgga tctagccaac agccagcgag
     8221 ctgcacttgc tgcctggctg gaagagtatc ccgaccaact tggcattgca ttaaccgact
     8281 gcatcactat ggatgctttc ctgcgtgatt tcggtgtcga gttcgctagt cggtatcagg
     8341 gcctgcgtca tgactctggc gacccggttg aatggggtga aaaagccatt gcacattatg
     8401 aaaagctggg aattgatcca cagagtaaaa cgctggtttt ctctgacaat ctggatttac
     8461 gcaaagcggt tgagctatac cgccacttct cttcccgcgt gcaattaagt tttggtattg
     8521 ggactcgcct gacctgcgat atcccccagg taaaacccct gaatattgtg attaagttgg
     8581 tagagtgtaa cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa actatctgcc
     8641 atgataaagc gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat attaaaaaag
     8701 ccagttaata tcatcaggga gctaatcggc tccctttttt tacctttaat tccgaaatct
     8761 ttcgctgcat ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca taggtatccc
     8821 ccccatttac gggtggacaa gtgtttattt tttccgacta ttaacagaga gaatattatg
     8881 agcgttgtgc ctgtagccga cgtactccag ggccgcgtag ccgttgacag cgaagtcacc
     8941 gtacgcggat gggtacgtac ccgccgagat tcaaaagctg gcatctcctt cctcgccgtt
     9001 tatgacggtt cctgctttga tcctgtacag gctgtcatca ataattctct gcccaattac
     9061 aatgaagacg tcctgcgtct gaccaccggc tgctcggtca ttgtgacggg taaagtcgtg
     9121 gcatcgccgg gccaggggca acaatttgaa attcaggcca gcaaggttga agttgctggt
     9181 tgggttgaag atccagacac ttacccgatg gcggcaaaac gccacagcat tgagtatctg
     9241 cgtgaagtcg ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc gcgcgttcgc
     9301 catacgctgg cgcaggcgct gcatcgcttc tttaacgagc agggattctt ctgggtttca
     9361 acgccactga ttaccgcatc tgataccgaa ggtgcaggcg aaatgttccg cgtttctacg
     9421 ctggatctgg aaaacctgcc gcgtaacgat cagggcaaag tggatttcga caaagacttc
     9481 tttggtaaag agtctttcct gaccgtatct ggccagttga acggcgaaac ctacgcttgc
     9541 gcattatcca aaatttatac cttcggcccg actttccgtg ctgaaaactc caacaccagc
     9601 cgtcacctgg cggaattctg gatgctggag ccggaagtgg cgtttgctaa cctgaacgat
     9661 atcgcgggtc tggctgaagc catgctgaaa tatgtcttca aagcggttct cgaagaacgc
     9721 gcagacgaca tgaaattctt cgctgaacgc gtagataaag atgccgtttc acgtctggaa
     9781 cgcttcattg aggccgattt cgcgcaggtg gattacaccg acgcagtgac cattctcgaa
     9841 aactgcggca ggaagtttga aaacccggtt tactggggcg tcgatctctc ttctgagcat
     9901 gagcgttatc tggcggaaga acactttaaa gcaccggtag tggttaaaaa ctatccgaaa
     9961 gatattaaag cgttctatat gcgcctcaac gaagacggta aaaccgttgc ggctatggac
    10021 gttctggctc cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga acgtctggac
    10081 gttctggacg agcgtatgct ggaaatgggc ctgaataaag aagattactg gtggtatcgc
    10141 gatctgcgtc gctacggtac tgttccgcat tcaggtttcg gtcttggttt tgaacgcctg
    10201 attgcttacg taactggcgt gcaaaacgtg cgtgatgtga ttccgttccc acgtactccg
    10261 cgcaacgcca gcttctgatt ttcttcctca aagagccagc gcctgctggc tcttttttat
    10321 cctttctgtc aagttatctg tttgttaagt caagcaatct atttgcaacc ccgccataac
    10381 gatcatcctg ttacggaata ttacattgca acatttacgc acaaaaaata acccgcattc
    10441 ttattgcgga ttagtttttt cttagctgat agcacaattt tcatactatt ttttggcatt
    10501 ctgga
//
LOCUS       DAZRVY010000136        10455 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZRVY010000136 DAZRVY010000000
VERSION     DAZRVY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.136"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHXOX_003516"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHXOX_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR7006892.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXOX_003517"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXOX_003517"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR7006893.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXOX_003518"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXOX_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR7006894.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXOX_003519"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXOX_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR7006895.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXOX_003520"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXOX_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXOX_003521"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXOX_003521"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR7006896.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXOX_003522"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXOX_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR7006897.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXOX_003523"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXOX_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR7006898.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHXOX_003524"
     CDS             complement(9457..10149)
                     /locus_tag="ACHXOX_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR7006899.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2556 a         2522 c         2764 g         2613 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg ggggcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZRVY010000137        10311 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZRVY010000137 DAZRVY010000000
VERSION     DAZRVY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10311
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.137"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..825
                     /locus_tag="ACHXOX_003525"
     CDS             <1..825
                     /locus_tag="ACHXOX_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFR7006900.1"
                     /translation="MNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSF
                     GGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMA
                     ALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDK
                     AGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIY
                     KNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKTGEPPNYLYL"
     gene            1161..2138
                     /gene="glaH"
                     /locus_tag="ACHXOX_003526"
     CDS             1161..2138
                     /gene="glaH"
                     /locus_tag="ACHXOX_003526"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR7006901.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2158..3426
                     /gene="lhgO"
                     /locus_tag="ACHXOX_003527"
     CDS             2158..3426
                     /gene="lhgO"
                     /locus_tag="ACHXOX_003527"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR7006902.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3449..4897
                     /gene="gabD"
                     /locus_tag="ACHXOX_003528"
     CDS             3449..4897
                     /gene="gabD"
                     /locus_tag="ACHXOX_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7006903.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4911..6191
                     /gene="gabT"
                     /locus_tag="ACHXOX_003529"
     CDS             4911..6191
                     /gene="gabT"
                     /locus_tag="ACHXOX_003529"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR7006904.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            6502..7902
                     /gene="gabP"
                     /locus_tag="ACHXOX_003530"
     CDS             6502..7902
                     /gene="gabP"
                     /locus_tag="ACHXOX_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR7006905.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7923..8585
                     /gene="csiR"
                     /locus_tag="ACHXOX_003531"
     CDS             7923..8585
                     /gene="csiR"
                     /locus_tag="ACHXOX_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR7006906.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8586..9035)
                     /gene="kbp"
                     /locus_tag="ACHXOX_003532"
     CDS             complement(8586..9035)
                     /gene="kbp"
                     /locus_tag="ACHXOX_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR7006907.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9119..9277)
                     /locus_tag="ACHXOX_003533"
     CDS             complement(9119..9277)
                     /locus_tag="ACHXOX_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR7006908.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9460..9759
                     /locus_tag="ACHXOX_003534"
     CDS             9460..9759
                     /locus_tag="ACHXOX_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR7006909.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9769..10293
                     /gene="ygaP"
                     /locus_tag="ACHXOX_003535"
     CDS             9769..10293
                     /gene="ygaP"
                     /locus_tag="ACHXOX_003535"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR7006910.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2447 a         2598 c         2772 g         2494 t
ORIGIN      
        1 atgaattaca tggaatatct ggtaacttgt cagttggatg aacaacaaat gtcatcactg
       61 ctttatgaaa gagatgattt aagcgccatt gatttttcat tgctcatgaa gacgataaaa
      121 gccttttcat tcgggggaga tctcctaacc cttgcatcaa aaccgggttc cacaatctca
      181 agcatcccat cagaaagacg gatattgatt aacattaacc acgattttcc taacaatggc
      241 aatcttttca atgactttct atttaaccat caacaagatg aacaattagc aatggcatat
      301 atggccgctc tcccgttcag cagaccttta gtttactggg atggccaggt attaaaatca
      361 acgactgaaa ttaaaaatta tgatgggtcc acgcgtgtcg gtggtgaggc gtggcttaat
      421 aaaggttgct ctacctatca gcagctctac aatgaattcc acgcattata tatagataaa
      481 gcaggaatat ggagcgcatt tgagggtgta tttgcaacta aaaacgttct ggcctttagt
      541 cgtggggatt ctgtgaacat taatcactct cctcatgatg gactagttat aataaataaa
      601 ggaaacgaag aagttgaagg tacctggcct aacaaattgc aacctggaat atataaaaac
      661 atggggagta atagcgttaa cattattatt aataataccc gaaaaattat cccccctggt
      721 aaagtattta cgcttagagg cggaactcta aatatcaata ttcctgggcg tagcgctctt
      781 cttttaggga aaacaggaga accgccgaac tatctctatt tataatttaa tgttatatct
      841 gccccgataa aacggggcag ataatatgtt tagtttacta acggtcattt tgcagtgaag
      901 ccatttactg ttttttatcg accagataat ctgttctcta atgttaactc cccctaacct
      961 gttgctttag ttattcattt cctgtctcac tttgccttaa tactctacgt taaatgttac
     1021 taatttgttg cttttgatca caataagaaa acaatatgtc gcttttgtgc gcatttttca
     1081 gaaatgtaga tatttttaga ttatggctac gaaatgagca tcgccatgtc accctacatc
     1141 tcataagagg atcgcttctg atgaatgcac tgaccgccgt acaaaataac gctgtcgatt
     1201 caggccagga ctatagcgga ttcactctca tcccgtcggc gcaatccccg cgtctgctgg
     1261 aactcacctt caccgaacag acgaccaaac agtttctcga gcaggttgcc gaatggcccg
     1321 tacaggcgct ggagtacaaa tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc
     1381 tgtgtgcgaa tcagctgcaa ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg
     1441 cgctgttgat caatgcggtg ggtatcgatg atgtggcgca ggcagatgag atggtgaagc
     1501 tggcaacggc ggtagcgcat ctgattggtc gctcaaattt cgatgcgatg agcggtcagt
     1561 attacgcgcg tttcgtggtg aaaaatgtcg ataactcaga cagctatctg cgtcagccgc
     1621 accgcgtgat ggaactgcac aacgacggca cgtatgtcga agagatcacc gattacgtgc
     1681 tgatgatgaa aatcgatgag caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg
     1741 atgactggga acatctggac cactatttcc gccatccact ggcgcgtcgc ccgatgcgct
     1801 ttgccgcgcc gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg
     1861 atcaacaggg tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagattttg
     1921 aagaaggcgt gtggttgagc gagctttccg atgccattga aaccagcaaa ggcattcttt
     1981 ctgtacccgt tcccgttggc aaattcctgt tgataaacaa cctgttctgg ctgcacggtc
     2041 gcgaccgctt tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt
     2101 tcgcttacgc cactcaccac taccagacgc atcagtaagc gcaaaggaat tgagcggatg
     2161 tatgattttg tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt
     2221 gatgtttatc cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcac
     2281 cagacgggcc acaacagcgg cgtgatccat gccggggtct attacacgcc cggcagcctg
     2341 aaggcgcagt tttgcctggc ggggaaccgc gccaccaaag ccttttgcga tcaaaacggc
     2401 attcgctatg acaactgcgg caagatgctg gtcgccacgt ctcagctcga aatggaacgg
     2461 atgcgcgcct tatgggaacg cacagcggcg aacggtatcg agcgcgagtg gataaacgcc
     2521 gtggaactgc gcgagcgcga accgaatatc accgggcttg gtggcatttt tgtgccgtcc
     2581 agcggcattg tcagctaccg cgaagtcaca gcggcaatgg caaaaatctt ccaggccaga
     2641 ggcggcgaga ttatttataa cgccgaagtc agcgccctca gtgagcataa aaacggcgtg
     2701 gtgatacgca cccgtcaggg cggtgaatat gaagcatcga cgctgattag ctgttccggg
     2761 ctgatggctg accggctggt gaaaatgctc ggcctcgaac cgggctttat catctgcccg
     2821 ttccgtggtg agtatttccg ccttgcgccg gagcataacc agattgttaa ccacctgatt
     2881 taccccattc ccgaccccgc gatgccattt ttgggcgtgc atctcacccg catgatcgac
     2941 ggcagcgtga ccgtcgggcc aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag
     3001 cgcgacttct catttagtga cacgctggag attttgagct cgtcggggat tcgccgggtg
     3061 ctgcaaaacc atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc
     3121 tacctgcggc tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg
     3181 cccgccggtg tgcgggcgca ggcagtatcg ccggacggca agctgattga cgattttctg
     3241 tttgtcacca ccccacgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca
     3301 gcaattccta ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt
     3361 aaccccggac gcacgctgcg agcggcacgt agtgtggatg ccttacacgc cgcatttaat
     3421 caataacctt tgaaaacagg acgtaacaat gaaacttaac gacagtaact tattccgcca
     3481 gcaggcgttg attaacgggg aatggctgga cgccaacaat ggcgggatca tcgacgtcac
     3541 caatccggcg aacggcgaca agctgggtag cgtgccgaaa atgggcgctg atgaaacccg
     3601 cgccgctatc gacgccgcca accgcgccct gcccgcctgg cgtgcgctca ccgccaaaga
     3661 acgcgccaac attctgcgca actggttcaa tttgatgatg gagcatcagg acgatttagc
     3721 gcgcctgatg accctcgaac agggcaaacc actggccgaa gcgaaaggcg aaatcagcta
     3781 cgctgcctct tttattgagt ggtttgccga agaaggcaaa cgtatttatg gcgataccat
     3841 tcccggtcat caggccgata agcgcctgat tgttatcaag cagccgattg gcgttaccgc
     3901 agctatcacg ccgtggaact tcccggcggc gatgattacc cgtaaagccg gtccggcgct
     3961 ggcggcaggc tgcaccatgg tgctgaagcc cgccagtcag acaccgttct ctgcgctggc
     4021 gctggcggag ctggcgattc gcgcgggcat tccggctggc gtgtttaacg tagtcaccgg
     4081 ttcggcaggc gcggtcggta atgaactgac cagcaacccg ctggtacgca aactgtcgtt
     4141 taccggttcg accgaaattg gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa
     4201 agtgtcgctg gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga
     4261 taaagccgtg gaaggcgcgc tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg
     4321 cgccaaccgt ttatacgtgc aggacggcgt gtatgaccgc tttgccgaaa aattgcagca
     4381 ggcggtgagc aaactgcaca tcggcgacgg gctggataaa ggcgtcacca tcgggccgct
     4441 gatcgatgaa aaagcggtag caaaagtgga agagcatatt gccgatgcgc tggagaaagg
     4501 tgcgcgtgtg gtttgcggcg gtaaagcgga tgaacgcggc ggcaacttct tccagccgac
     4561 cattctggtg gacgtgccgg ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct
     4621 cgccccgctg ttccgcttta aagatgaagc cgatgtgatt gcgcaagcca atgacaccga
     4681 gtttggcctt gccgcctatt tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga
     4741 agcgctggag tacggcatcg tcggcatcaa taccggcatt atttccaatg aagtggcccc
     4801 gttcggcggc atcaaagcct cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga
     4861 ttacttagaa atcaaatata tgtgcatcgg tctttaactg gagaatgcga atgaacagca
     4921 ataaagagtt aatgcagcgc cgcagtcagg cgattccccg tggcgttggg caaattcacc
     4981 cgattttcgc tgaccgcgcg gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc
     5041 ttgatttcgc gggcgggatt gcggtgctca ataccgggca cctgcatccg aaggtggtgg
     5101 ccgcagtgga agcgcagttg aaaaaactgt cgcacacctg cttccaggtg ctggcttacg
     5161 agccgtatct ggagctgtgc gagattatga atcagaaggt gccgggcgat ttcgccaaga
     5221 aaacgctgct ggttacgacc ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg
     5281 ccgccaccaa acgtagcggc accatcgctt ttagcggcgc gtatcacggg cgcacgcatt
     5341 acacgctggc gctgaccggc aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg
     5401 gtcatgttta tcgcgcgctt tatccttgcc cgctgcacgg cataagcgag gatgacgcta
     5461 tcgccagcat ccaccggatc ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg
     5521 tgattgagcc ggttcagggc gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc
     5581 gtttacgcgc tctgtgtgac gagcacggga tcatgctgat tgccgatgaa gtgcagagcg
     5641 gcgcggggcg taccggcacg ctgtttgcga tggagcagat gggcgtggcg ccggatctta
     5701 ccacctttgc gaaatcgatc gcaggcggtt tcccgctggc gggcgtcacc gggcgcgcgg
     5761 aagtgatgga cgctgttgca ccgggcggtc tgggcggcac ctatgccggt aacccgattg
     5821 cctgcgtggc tgcgctggaa gtgttgaagg tgttcgagca tgaaaatctg ctgcaaaaag
     5881 ccaacgatct ggggcagaag ctaaaagacg gattgctggc gatagccgaa aaacacccgg
     5941 agatcggcga cgtacgcggg ctgggagcga tgattgccat cgagctgttt gaagatggcg
     6001 atcacagcaa accggacgca aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg
     6061 gcctgattct tctctcctgc ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca
     6121 ccattgaaga cgctcagatc cgtcagggtc tggagatcat cagccagtgt tttgctgagg
     6181 cgaagcagta gctatgtccg tttgtaagag ttaaccgttg ctaagggaat gataacgatg
     6241 cagtaaaaaa taataacgat gaggcgaacg acaaagttaa aaacgactgc ggacagtccc
     6301 ctcgcccctc tggagagagg gttagggtga ggggtaaaac acgcggctaa accctcccct
     6361 ggccctctcc ctaagggaga gggagaaaac cctcccgctt tcgtttttaa gattttgttg
     6421 accgtctcaa ttaaacgcgt gacccggcgc gggaaatgtc ggggcgctct cccaagtgac
     6481 acactttcga gaggattcag gatggggcaa tcatcgcaac cacatgagtt aggcggcggg
     6541 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     6601 gtcggttcca gcgtcgccat cgccgaagcg ggcccggcgg tattactggc ctatctgttc
     6661 gccgggctac tggtggttat gattatgcgg atgttggcgg aaatggcggt tgccacgccc
     6721 gataccggtt cgttttccac ctatgccgat aaagccattg gtcgctgggc gggctatacc
     6781 atcggctggt tgtactggtg gttttgggta ctggttatcc cgctggaagc caatatcgcc
     6841 gctatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     6901 acccttgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     6961 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     7021 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     7081 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     7141 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacacgcc ggaaaaacat
     7201 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     7261 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     7321 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     7381 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     7441 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     7501 ccgtacgtgg cggtgttact ctccaccggc gcggcattct taacggtggt ggtgaactat
     7561 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     7621 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcggaagga
     7681 agcgaaattc gcttgcgaat gtggctttat ccctggctca cctggctggt catcggcttt
     7741 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     7801 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
     7861 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
     7921 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
     7981 attttcaacc ggatgaaaaa ttacggatga gtttgctgac atcgcgttat gcacttggcg
     8041 ttgggccgtt gcgggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
     8101 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgatg
     8161 cccgcgccaa tatggaagcg atgttagtga gtctggcgat tgcccgcggt ggcgatgagt
     8221 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
     8281 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
     8341 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
     8401 gctatcgatt tatctggctg cggcgaacgg tgctttcggt agaaatgctg gaggataaac
     8461 acgatcagca ccagaccctg actgcggcgg tactggcgcg agataccgcg cgcgccagtg
     8521 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
     8581 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
     8641 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
     8701 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
     8761 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
     8821 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
     8881 tcggcaattt gaatgttcac tttatcagca tcaggtatac cggttttgct cagatgctcc
     8941 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcgtc ccagagtttt
     9001 tctccggcat ctttcacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
     9061 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
     9121 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
     9181 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgagcggcg
     9241 gtagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
     9301 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
     9361 tttcagctta cgcttttact ctcatttacg ctgctgaggc tggtatatgt tgcattttta
     9421 gtttagagct tacttaattt agaaaatact taaataaata tgactgaact cgcgcaatta
     9481 caggccagtg ccgaacaggc agcggcctta ttgaaagcaa tgagccatcc taaacgattg
     9541 ctgattctgt gcatgcttag cggttccccc ggcaccagcg cgggagagct gacgcgcatt
     9601 accggactga gtgcctctgc gacatcacag catctcgctc gtatgcggga cgaagggctt
     9661 atcgacagcc aacgggatgc ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat
     9721 gccattatcg ccaccctgaa aaatgtctat tgtccgtaag gagtcactat ggctttgaca
     9781 accatttcgc cgcatgatgc acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt
     9841 cgtgacgctg atgaatatct tcgtgaacat attcctgaag cagatctggc tccattatcc
     9901 gtgctggaac agtcaggtct tccacctaaa ttacgtcgcg agcaaattat attccactgc
     9961 caggcaggta aacgcaccag taataacgcc gataaattag cagcgattgc cgcccccgca
    10021 gaaatatttt tactcgaaga tgggattgat ggctggaaaa gagctggatt gccagtagcg
    10081 gtaaataaat ctcaaccctt gccgttaatg cgccaggtgc agatcgctgc gggtggttta
    10141 atattaatcg gcgttgtact gggttatacc gtaaatagcg gtttcttctt attaagtggc
    10201 tttgttggtg ccgggttact gtttgcagga atcagcggtt tttgtggaat ggcaaggttg
    10261 ttagataaga tgccgtggaa ccaacgagct tgagaagcga cgccggacgc g
//
LOCUS       DAZRVY010000138        10197 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZRVY010000138 DAZRVY010000000
VERSION     DAZRVY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.138"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHXOX_003536"
     CDS             <1..309
                     /locus_tag="ACHXOX_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR7006911.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHXOX_003537"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHXOX_003537"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR7006912.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHXOX_003538"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHXOX_003538"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR7006913.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHXOX_003539"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHXOX_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR7006914.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHXOX_003540"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHXOX_003540"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR7006915.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHXOX_003541"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHXOX_003541"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR7006916.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHXOX_003542"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHXOX_003542"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR7006917.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHXOX_003543"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHXOX_003543"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR7006918.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHXOX_003544"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHXOX_003544"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR7006919.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHXOX_003545"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHXOX_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR7006920.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHXOX_003546"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHXOX_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR7006921.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         2263 a         2836 c         2682 g         2416 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttc
//
LOCUS       DAZRVY010000139         9871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZRVY010000139 DAZRVY010000000
VERSION     DAZRVY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.139"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHXOX_003547"
     CDS             <1..347
                     /locus_tag="ACHXOX_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR7006922.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHXOX_003548"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHXOX_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR7006923.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHXOX_003549"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHXOX_003549"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7006924.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHXOX_003550"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHXOX_003550"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR7006925.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHXOX_003551"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHXOX_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR7006926.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHXOX_003552"
     CDS             complement(4344..5309)
                     /locus_tag="ACHXOX_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7006927.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHXOX_003553"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHXOX_003553"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR7006928.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHXOX_003554"
     CDS             6366..6746
                     /locus_tag="ACHXOX_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR7006929.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHXOX_003555"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHXOX_003555"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR7006930.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHXOX_003556"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHXOX_003556"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR7006931.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2294 a         2488 c         2559 g         2530 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaaa gttataaata aaaagagatt gtatttaaag tgcaaaaatt
     9841 caattgctaa taagttacat tttaataatg a
//
LOCUS       DAZRVY010000140         9859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZRVY010000140 DAZRVY010000000
VERSION     DAZRVY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.140"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHXOX_003557"
     CDS             complement(<1..185)
                     /locus_tag="ACHXOX_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006932.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIT"
     gene            263..1357
                     /gene="ddlA"
                     /locus_tag="ACHXOX_003558"
     CDS             263..1357
                     /gene="ddlA"
                     /locus_tag="ACHXOX_003558"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR7006933.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1381..1593)
                     /locus_tag="ACHXOX_003559"
     CDS             complement(1381..1593)
                     /locus_tag="ACHXOX_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR7006934.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1853..2161
                     /locus_tag="ACHXOX_003560"
     CDS             1853..2161
                     /locus_tag="ACHXOX_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR7006935.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2220..3314)
                     /gene="yaiW"
                     /locus_tag="ACHXOX_003561"
     CDS             complement(2220..3314)
                     /gene="yaiW"
                     /locus_tag="ACHXOX_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR7006936.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3327..4547)
                     /gene="sbmA"
                     /locus_tag="ACHXOX_003562"
     CDS             complement(3327..4547)
                     /gene="sbmA"
                     /locus_tag="ACHXOX_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR7006937.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4899..6056
                     /gene="ampH"
                     /locus_tag="ACHXOX_003563"
     CDS             4899..6056
                     /gene="ampH"
                     /locus_tag="ACHXOX_003563"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR7006938.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6057..6680)
                     /gene="iprA"
                     /locus_tag="ACHXOX_003564"
     CDS             complement(6057..6680)
                     /gene="iprA"
                     /locus_tag="ACHXOX_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR7006939.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6768..9656)
                     /locus_tag="ACHXOX_003565"
     CDS             complement(6768..9656)
                     /locus_tag="ACHXOX_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR7006940.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2550 a         2426 c         2362 g         2521 t
ORIGIN      
        1 aaagttatga aatatattct cttcgttaac ttataattaa gagaaaaaaa ctcaccgcat
       61 tgccatttaa gccaggaata aattagcttc ccgttcatca tttttgtgat ctcgcgcaca
      121 tctttgagat aacgcacttt cttcaccagt acgttgcacg ctttgtcagc agaagattgc
      181 aacaatcttt cagaaggccg cgaatccccc gttttgtcgt ggtaatgtat gcactttgtg
      241 ttagacggga taggttttta agatggaaaa actgcgggta ggaatcgttt ttggtggtaa
      301 atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac attgtcgatg ccattgataa
      361 aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa gggcaatggc acgtcagcga
      421 tgccagcaat tatctgctaa atgcagacga tcctgcccat attgcgttgc gcccttcggc
      481 gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag cttatcgacg cgcaaaacgg
      541 tcagccgttg ccgacggtgg atgtcatttt cccgattgtc cacggtacgc tgggcgaaga
      601 tggttccttg cagggaatgc tgcgggtcgc caatttaccg tttgtaggtt ctgatgttct
      661 ggcttcagca gcctgtatgg ataaagatgt caccaaacgt ctgctacgcg atgccgggct
      721 gaacattgcg ccatttatta ccctgacgcg cgctaatcgt cacaacatca gttttgccga
      781 agtggagtct aaactggggt taccactgtt tgtaaaaccg gctaatcagg gctcttctgt
      841 tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt gccgtcgatc tggcgttcga
      901 gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt cgtgagatcg aatgcgcagt
      961 tctgggcaac gacaatccgc aagccagcac ctgtggcgag atcgtactca ccagcgattt
     1021 ctatgcctac gacaccaagt acattgacga agatggcgcg aaagtggtag ttccggcagc
     1081 cattgcacca gagatcaacg ataagatccg ggcgattgcc gttcaggctt atcagacgtt
     1141 gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc ccagagaacg aagtggtgat
     1201 caacgagatc aacacactgc ctggcttcac caacatcagt atgtatccga agctgtggca
     1261 agccagcggt ctgggttaca ccgatctgat cacacgtttg attgagctgg cgctggagcg
     1321 tcacgctgcg gataacgcat tgaaaaccac aatgtaatat tttaacgccc gataatattc
     1381 ttattgggcg ttttcagttt cttcatcgcg acgacgaata atcaaccccg ccagccagaa
     1441 actaatcacc cacgtcacca gaccgacggc ataggtctgc caaccttttg cttcaaatcc
     1501 cagtaacccc accacgccat taagaatgaa tataaggcca atggcgaacg catagtagtg
     1561 ccagtcacgg cggattttta caggcagatt catggcagct ccgttaacga aaaacaacat
     1621 ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac attttcgtaa ttgttaataa
     1681 atttaactta aggttgccat tttgtaactc acaacgaaga ttcacgcttc gggaatattc
     1741 ccaacatcaa tttaccatga ttctgatatt gacaatctaa atgacctgtc agacttgcca
     1801 taagccgctg gtatcacacc agaagagaag cagatgactt ccggaggttt ttatggctga
     1861 tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc aatgcctctt caacgcctgg
     1921 caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg gctggggcgc aattgctgaa
     1981 cctgttagtg catgcgcccg gcgtctatga gcgtttaatg caggtccagg aaacaggtcg
     2041 cccacgggtg gaaattggtt taggtgtcgg caccattttc gggctgatcc cgtttttagt
     2101 aggctgcctc atttttgcag tggtggcgct atggctgcac tggcgacatc gccgccagta
     2161 agatttacgt tgccagatac ggcctaaaac gccttatctg gcatgacatt cctgtttgat
     2221 cactgtttca tacaacgcgc ccgccgttcg tctacgcgct tcgcaaacca ggccgtagtc
     2281 aggttgcggg tgattttcgg gctttccagt tgaatgccag gcaacatttc gcggggtagt
     2341 gatttacccg ttttcgcttc ggcaagttga taaactttct tgtacagcgc cgtctcctca
     2401 aaagagaagc tgtcaccttt ctctaactga cggcgaattt cgctgtcgct catccccagt
     2461 tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg gttctttact gtcgtaacga
     2521 attaaatcac cgtccagtgc cagcttcacg ccgctggcct tactgactgc gttctgaaac
     2581 gcggcattac ggctggcgta ccagccagcg ttaaaatcag caaagcggta tatcggtgcg
     2641 ctatagctgg cgggataatt cagtaaatgg taagtaccaa accacaaccc gccgcggcga
     2701 ctgaagactt cctgacgaac tgtaccgtcc attttccacg gatacccttt ggtatgctgt
     2761 tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc gcaccggatt aagcgaacca
     2821 aacagcgtct gccccattgg taccatgctg atcagatcgt caaaaatcgc gctcaattgc
     2881 ttttctgtac gcaccgaatc taaccgttcg ctataacttt tgccgtttgg cgatttgatt
     2941 tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc gttcagcacg acggtcaatc
     3001 tcttgccagg cgatcttgct taagcccgga acggccggat ctgcctgata atttgactcc
     3061 tgctgcgcca ccgccagcac cgagcagacg ttttccagcg tcggtgccag cccctgactt
     3121 tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt ctttcacgct cgcaggcatc
     3181 ttctgccgca cgacgctcgc tacatctatc gctttttcgc cctttttcaa cggttgcgga
     3241 gcctgactac tacaccccgc taaaaccaga accgccaaca atgacagcga agatagatga
     3301 ttgacgcgcg acattacttc tcctttttag ctcaaggtat gggttacttc ctgaattttg
     3361 tcaccatcca gctcatgttc aaagctgcgc agacgtttgt agatagacat caactcaacc
     3421 agtgtggtcc atgagttaat caggtactgg aaagcaccgc gaacctgacc aaaaacgttg
     3481 gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa caattgacgg aaacagcaag
     3541 aacaaaccga aaacgttatc gacctgcaaa tagagaatgc gggcgatgtt gaaatacata
     3601 tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc taaacagctc gcgtaccgta
     3661 ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca gctctttacg gtaggcggcc
     3721 tctactcgct ggtttttaaa ctccagcccc ggcagtttga tccctaccac tgccagccat
     3781 ccggtcccca tcagcgacca gacgatagcg gcaatcacca gaccatacgg aatgtgcccg
     3841 ataatcggca gctccggcac atgcgcggag agcgttacca gcaccggcag gaaggcgatc
     3901 aacgtcatga tggcgttgat aaaactgacg cccatattct ccagcgttga agcaaaacgc
     3961 atggtgtctt cctgcacacg ctgtgcggca ccttcgatat gacgcagttg ttgccagttc
     4021 gccatgtaat attcgttcat cgccgtacgc cagcggaaca catagtgact gacaaagaag
     4081 ttgttcagta cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg
     4141 cggtaaaatt gttcgatagt gactttatgc ggcgaactta gcgcggtttg aatcaaatca
     4201 tagaacggtg cataccaggc gttgacggcg accccgactt ccaccaaaaa ccaggtgacg
     4261 aagatgatca gtgcagtacc gagtattgac cagtattgcc aacggtgcgg gctgtagata
     4321 aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa
     4381 tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc
     4441 gccacccagt ctcccccacc ggcttgccag aagataacgg caatcaatgc ccaaacaaag
     4501 gccgagagaa aaaacgctcc cggctttggg aaaaaagact taaacatatg cactcctgct
     4561 aacttcttat cgttttaaca actgctgtgt attaaccgca tgaccactac aaagcgtcag
     4621 ccaaatgaaa ccgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata
     4681 agaattgttt cgacggattt cacaaaccaa agattgttta cataagcgga ttaacacgcg
     4741 caggatcaca aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg
     4801 tttttaacac tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat
     4861 acgctcagtt accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt
     4921 ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc ggcgcagccg atcactgaac
     4981 cggagtttgc ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg
     5041 caacggggat ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgt agttatggcg
     5101 aaacgcgacc tggtaataac gttcgcccgc agctggattc ggtcgtgcgt attgcttccc
     5161 tcaccaaact aatgaccagt gaaatgctgg tgaaattgct cgaccagggg accgtaaagt
     5221 taaacgatcc gctaagtaaa tatgctccgc caggcgcacg cgtgccgacc tacaatggga
     5281 cgcccattac cctagtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg
     5341 gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg cgagcaacgc tggaaatacc
     5401 tttctacggc gaagctgaaa gccgcgccag gcagtcaggc ggcgtactct aaccttgcgt
     5461 tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg
     5521 aagagcagat tacccgtcca ttggggatga aagacaccac ctacaccccc tcaccggatc
     5581 agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg
     5641 caattggcag cggcggcgtc tattccacgc ctggcgatat gatgcgctgg atgcagcagt
     5701 atttgtcatc tgatttttat cagcgcagta accaggcaga tcgcatgcag acgttgattt
     5761 atcagcgcgc gcagtttacg aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc
     5821 ttggtttagg ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga
     5881 caggcggtgg tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg
     5941 cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca
     6001 atgatctggt gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa
     6061 tactctgaag gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg
     6121 cctttacgca aagcagcgag aacttctgcg acgacagaac gcgatatgcg tgtacgttga
     6181 tggatataat tcatcacacc aatacgtgat cgcaattctt cattccagtc aatcattgat
     6241 aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa tgagctgcac gtcgcgtaat
     6301 tccagaattc tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt
     6361 tcaataagcg taatggtttg tttggctggt agatgatatc ccgtacaatt tccttctgat
     6421 attaatttgt atggtatatc gtttttcatt aaaccatcag ccagccccat aatataaggg
     6481 gcctgggtaa taccgataag tacgttttct tctctacgca gagagataac tccctctaaa
     6541 ataacgaaag tatcttcgtt attgacatca ctggaaaata taacttgctt ttcattatta
     6601 aactcgaagc gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga
     6661 ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg
     6721 ggcattattt agattaaatt accagtattt cttcggagtg aagaatatta ccaggtatat
     6781 ttaacaccca cgttcgcgga ccagtcttga tctacgtcac caccaccgag gtagttagca
     6841 tcggtatagg cgctgaagtt cttggtgaag ctgaactgag tacccagacc aacacgtacc
     6901 gcagaccctt cagtaccgtt atcgatggaa tcaccgttca catcgttatc gttgttagag
     6961 tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag cctgatcttc gctgtaggtg
     7021 aaggtataac ctgcatctac acccagttca taacgcatgc tgtcgtaaga ctgaccgtca
     7081 actttcatgt cgttgctcag ctggtagtca tcaccagact ggaacagacc agaaacgctg
     7141 ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt cgtaaccggc tttcaagccg
     7201 aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag taccgttgct catggttgca
     7261 gacaggtcgt tgttgaagtg agagtagctc aagctaccat caacaaagac gttgttcgcg
     7321 aagtgagcag aagagtagat gtaggcagtc tggctgtctt gatctacctg accagaacgg
     7381 tcattcatgt cacctttagc gaagcctgca gccgcaccga cgatccactt agcgttgtta
     7441 ccgtcaactt tggtatcaac accgaccatg atgccgttaa catcctgatc atagttgatg
     7501 gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc gccgttatca
     7561 gccaggccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc caggttccag
     7621 atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt cagctccatc
     7681 tgttgcagaa caacggtgtt accgcgctgt tcagcctgat aggtgtatgc acccaggtca
     7741 gctttgttag cagcagagaa ggtcgcgttg gttttggtat catcgatata gatgagctgc
     7801 ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt tgtcgatacg aactttgtag
     7861 ttaccggttg cagctacaac gttgtctgca gagctgttgt caacttcgtc gccattgtta
     7921 atgtcgttgt taccattaac ggtcaagtga ccatcagagt tcatcgcaat aacaccataa
     7981 ccgtagttag ctttgctggt gtcgttggta cgatcgttga cgaggtctgc attcaggatg
     8041 taatcattac tatgaatgtc aaacacacct gcgtaaacat taccgttgtt atctttaacg
     8101 ttgccactgg tcagttccag agtatcagtt gcaaatacgc tggagatgtc attcatgttg
     8161 gtgtttacat ccagcacacc accgttagta acggtaatgg tattggcata cagcggtgca
     8221 tatgcccaag ctgaagcagt atcttcaccg aagttaacgg ttgagaaggt atcaatggtc
     8281 aggtgatcgg tgttaacttc accgttaact tccattgaag aaccaccgtt cagagtgatg
     8341 gtgtcagaag tcagagaaga atctgcactc acgttaacct gagaaccgct gttgatcgcc
     8401 agggtatcga tcagggaagt tttcgtggtt tcccaatcag aaccgttgtt cagaacaaca
     8461 tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac gattccagcc atcttcagag
     8521 taagaagcgt acgggttcag gctgtttggt gcaacgtcgt agaaggcttc gtcagcaaca
     8581 acaacgtcat aatcaatatt cgctgaacca acccatttgg tgttgttcag agtcaggttc
     8641 aggtgatcga cgttctgatc gtcagttgcc caaccgccat gagtgtaagc agtggtagag
     8701 ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc aggtactgtc aaatacaacg
     8761 tcaccaagca actgagagtt gttgaacgta acgttgttga ccatacgata atcagaagtg
     8821 tcatcatcga tgaaggctaa cgcaacatca tcagcaggag atgcgccatt gccgtagtca
     8881 cttggttcaa cagaattgcc gaaatgacca cgatctgatt gttcgtcttc agtccatgaa
     8941 ccagaagtta cagtagagtc attaacaacg atgttgttgt cccactgatg accttcggta
     9001 ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa tacctgcaac atgggagttg
     9061 ttgctgatgt tgatgttact ttcaacatcc agagtcacag cggtaccaag accagcggtt
     9121 accaaacgat cataatctgt cttagatgtt ttaccgtctg catccagata agtatcggtg
     9181 aagtagaagg cgtcaaaatc atcgtcaata gtagaattag cgatgttcag agtgaagatg
     9241 tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt tggtccagac gtagtcgcca
     9301 taaccaacct ggtaagatgt aaccatacca tgaacaattg agttggagat gttcagtgta
     9361 ttaccggtat ctgtgccata atcgtggttc agataataag tagaaattac accatttaca
     9421 gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg cgtggtcata tacataacca
     9481 ccgtaagtca gataatcgtg atcaatttta gtgctgtcac cccaattgta gatggtggct
     9541 gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat tagcctgaga ggtgatagcc
     9601 agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga gtgcatttgt
     9661 catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata gaaaacatat
     9721 tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta ttctttcccc
     9781 taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag
     9841 gacgatattt caacatgtt
//
LOCUS       DAZRVY010000141         9674 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZRVY010000141 DAZRVY010000000
VERSION     DAZRVY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.141"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHXOX_003566"
     CDS             <1..1710
                     /locus_tag="ACHXOX_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR7006941.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHXOX_003567"
     CDS             1711..3360
                     /locus_tag="ACHXOX_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR7006942.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHXOX_003568"
     CDS             3365..4141
                     /locus_tag="ACHXOX_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR7006943.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHXOX_003569"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHXOX_003569"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR7006944.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHXOX_003570"
     CDS             complement(5430..6752)
                     /locus_tag="ACHXOX_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR7006945.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMELIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHXOX_003571"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHXOX_003571"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR7006946.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHXOX_003572"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHXOX_003572"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR7006947.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHXOX_003573"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHXOX_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR7006948.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2483 c         2604 g         2233 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagctc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcttgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZRVY010000142         9645 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZRVY010000142 DAZRVY010000000
VERSION     DAZRVY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.142"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHXOX_003574"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHXOX_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR7006949.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHXOX_003575"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHXOX_003575"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR7006950.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHXOX_003576"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHXOX_003576"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR7006951.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHXOX_003577"
     CDS             complement(5698..7014)
                     /locus_tag="ACHXOX_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR7006952.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHXOX_003578"
     CDS             complement(7004..8761)
                     /locus_tag="ACHXOX_003578"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR7006953.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="ACHXOX_003579"
     CDS             complement(8777..>9645)
                     /locus_tag="ACHXOX_003579"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR7006954.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAZRVY010000143         9621 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZRVY010000143 DAZRVY010000000
VERSION     DAZRVY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.143"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHXOX_003580"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHXOX_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHXOX_003581"
     CDS             2192..3328
                     /locus_tag="ACHXOX_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR7006955.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHXOX_003582"
     CDS             3325..5325
                     /locus_tag="ACHXOX_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR7006956.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEVARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="ACHXOX_003583"
     CDS             5450..5911
                     /locus_tag="ACHXOX_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR7006957.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="ACHXOX_003584"
     CDS             complement(5952..6422)
                     /locus_tag="ACHXOX_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR7006958.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHXOX_003585"
                     /gene_synonym="yehT"
     CDS             complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHXOX_003585"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR7006959.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHXOX_003586"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHXOX_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR7006960.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="ACHXOX_003587"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="ACHXOX_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9385..>9621
                     /locus_tag="ACHXOX_003588"
     CDS             9385..>9621
                     /locus_tag="ACHXOX_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR7006961.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2353 a         2531 c         2469 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagagg tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg ctgacgagtg g
//
LOCUS       DAZRVY010000144         9501 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZRVY010000144 DAZRVY010000000
VERSION     DAZRVY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.144"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHXOX_003589"
     CDS             <1..108
                     /locus_tag="ACHXOX_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR7006962.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHXOX_003590"
     CDS             complement(149..1231)
                     /locus_tag="ACHXOX_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR7006963.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHXOX_003591"
     CDS             complement(1228..3060)
                     /locus_tag="ACHXOX_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR7006964.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHXOX_003592"
                     /pseudo
     CDS             complement(3275..3631)
                     /locus_tag="ACHXOX_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3689..4111)
                     /locus_tag="ACHXOX_003593"
     CDS             complement(3689..4111)
                     /locus_tag="ACHXOX_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7006965.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHXOX_003594"
     CDS             complement(4127..4852)
                     /locus_tag="ACHXOX_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR7006966.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHXOX_003595"
     CDS             complement(4874..5620)
                     /locus_tag="ACHXOX_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7006967.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHXOX_003596"
     CDS             complement(5627..6691)
                     /locus_tag="ACHXOX_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR7006968.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHXOX_003597"
     CDS             complement(6763..7188)
                     /locus_tag="ACHXOX_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR7006969.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHXOX_003598"
     CDS             complement(7185..7448)
                     /locus_tag="ACHXOX_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7006970.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHXOX_003599"
     CDS             7560..8060
                     /locus_tag="ACHXOX_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7006971.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHXOX_003600"
     CDS             8080..8274
                     /locus_tag="ACHXOX_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR7006972.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHXOX_003601"
     CDS             8274..8564
                     /locus_tag="ACHXOX_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR7006973.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHXOX_003602"
     CDS             8844..8996
                     /locus_tag="ACHXOX_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR7006974.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHXOX_003603"
     CDS             9008..9373
                     /locus_tag="ACHXOX_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7006975.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2444 a         2491 c         2016 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctagcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZRVY010000145         9496 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZRVY010000145 DAZRVY010000000
VERSION     DAZRVY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.145"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHXOX_003604"
     CDS             122..952
                     /locus_tag="ACHXOX_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR7006976.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXOX_003605"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXOX_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR7006977.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHXOX_003606"
     CDS             complement(2081..3541)
                     /locus_tag="ACHXOX_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR7006978.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXOX_003607"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXOX_003607"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR7006979.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHXOX_003608"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHXOX_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR7006980.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHXOX_003609"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHXOX_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR7006981.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHXOX_003610"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHXOX_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR7006982.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHXOX_003611"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHXOX_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR7006983.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHXOX_003612"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHXOX_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR7006984.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZRVY010000146         9402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZRVY010000146 DAZRVY010000000
VERSION     DAZRVY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.146"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHXOX_003613"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHXOX_003613"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR7006985.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHXOX_003614"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHXOX_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR7006986.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHXOX_003615"
     CDS             1393..1914
                     /locus_tag="ACHXOX_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7006987.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHXOX_003616"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHXOX_003616"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR7006988.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHXOX_003617"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHXOX_003617"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR7006989.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHXOX_003618"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHXOX_003618"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR7006990.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHXOX_003619"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHXOX_003619"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR7006991.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHXOX_003620"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHXOX_003620"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR7006992.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHXOX_003621"
     CDS             complement(6556..7449)
                     /locus_tag="ACHXOX_003621"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR7006993.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHXOX_003622"
     CDS             complement(7446..8099)
                     /locus_tag="ACHXOX_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR7006994.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHXOX_003623"
     CDS             complement(8110..9369)
                     /locus_tag="ACHXOX_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR7006995.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2092 a         2667 c         2494 g         2149 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aaaccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cg
//
LOCUS       DAZRVY010000147         9122 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZRVY010000147 DAZRVY010000000
VERSION     DAZRVY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.147"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHXOX_003624"
     CDS             <1..302
                     /locus_tag="ACHXOX_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7006996.1"
                     /translation="EFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSGG"
     gene            299..628
                     /locus_tag="ACHXOX_003625"
     CDS             299..628
                     /locus_tag="ACHXOX_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7006997.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            628..1326
                     /locus_tag="ACHXOX_003626"
     CDS             628..1326
                     /locus_tag="ACHXOX_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR7006998.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            1337..2080
                     /locus_tag="ACHXOX_003627"
     CDS             1337..2080
                     /locus_tag="ACHXOX_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7006999.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            1978..2658
                     /locus_tag="ACHXOX_003628"
     CDS             1978..2658
                     /locus_tag="ACHXOX_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR7007000.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            3002..6453
                     /locus_tag="ACHXOX_003629"
                     /pseudo
     CDS             3002..6453
                     /locus_tag="ACHXOX_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            6521..7120
                     /locus_tag="ACHXOX_003630"
     CDS             6521..7120
                     /locus_tag="ACHXOX_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR7007001.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            7272..>9122
                     /locus_tag="ACHXOX_003631"
     CDS             7272..>9122
                     /locus_tag="ACHXOX_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7007002.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIE"
BASE COUNT         2158 a         2318 c         2818 g         1828 t
ORIGIN      
        1 gggagtttgt cttcacgaag gaggcgacca gccggattgg tgtcggcaat ctgtaccgcc
       61 tgatgcgggg ctatgcggaa ggtggttatg tcggcggtgc cggaagtccg gcgcagatgc
      121 ggcgggcgga aggcattaat tttaatcaga acaatcacgt ggtgattcag aacgacggcc
      181 ccaacgggcg ggcagggccg cagctgatga aagcggtgta tgagatggcc cgcaaggggg
      241 cacaggatga actccggctg cagttgcgtg atggcggtat gttatcaggg agcggtggat
      301 gaaaaccttt cgctggaaag tgaagccgga tatggaggtg aactcgcagc catcggtgcg
      361 tgaagtgcgt tttggtgacg ggtactcaca gcgtatggcg gcagggctga atgctgacct
      421 gaaaacatac agggtgacgc tttccgtgac ccgggaggag gcccggcatc tggaagcgtt
      481 cctggcagag cacggtggct ggaaggcatt tttgtggaag ccaccctatg cataccggca
      541 gataaaggtg acctgtgccg ggtggtctgc gcgggtcggg atgttgcgcg ttgagttcag
      601 cgcggagttt aagcaggtgg tgaactgatg caggatattc gcgaagaaag tctgaacgag
      661 tcggttaagt cagagcagtc accgcgggtg gtactctggg aaatcgacct gacggtacag
      721 ggtggtgagc ggtatttttt ctgtaatgag ctgaatgaaa aaggggagcc ggtcacctgg
      781 caggggcgta agtatgaggc atacccgatt gacggcagcg gctttgagat gaacggccgg
      841 ggcagcagtg ccagaccgtc gctgacggtg tccaatctgt tcggtctggt caccgggatg
      901 gcggaagacc tgcagagtct ggtgggggcc acggtggtcc gccgccgggt gtatgcccgt
      961 tttctggatg cggtgaattt cgttgcgggc aatccggagg cggacccgga gcaggagctg
     1021 agtgaccgct gggtggtgga gcagatgtcg cagctgacag ccatgacggc ctcgtttgtg
     1081 ctggctacac cgaccgagac ggacggggcg ctgtttcccg gtcgcatcat gctggcgaac
     1141 acctgtatgt gggattaccg gggagatgaa tgcgggtata acggtcctgc ggtggcggat
     1201 gagttcgaca accccaccac ggatatccgt aaggacagat gcagcaagtg catgcgcggg
     1261 tgtgaactgc gcaggaatgt cggcaatttt ggcggtttcc tttccattaa taaactttcg
     1321 cagtaaatcc cggtttatga cacagactga atcagcgatt ctggcacatg cccggcggtg
     1381 tgcgcctgcg gagtcgtgcg gcttcgtgat aagcacgccg gagggggagc ggtatatccc
     1441 ttgtgtgaat atttccgcag agccggaggc gtattttcgt atcgcaccgg aagactggct
     1501 gcgggcagag atgcaggggg agattgtggc actggtccac agtcatcccg gtgggctgcc
     1561 ctggctgagc gaggctgacc ggcggttgca gataaaaagc gcactgccct ggtggttggt
     1621 ctgccggggt gacattcaca aattccgctg tgtgccacat ctgacaggac ggcgctttga
     1681 gcacggggtg acggactgtt acacgctgtt ccgggatgct tatcatctgg cggggactga
     1741 aatgccggat tttcatcgcg aggatgactg gtggcgcaac ggtcagaacc tttacctgga
     1801 caatatggcg gtcaccggct tttaccgggt gcccctgtcc tctgcacagg cgggcgatat
     1861 tctgctgtgc tgctttggtg cttcggtacc gaaccatgcc gccatttact gcggcaacgg
     1921 tgagctgctt caccatctgc ctgaacaact gagtaaacgg gagaggtatt ccgaaaaatg
     1981 gcaacgacga acgcattcag tctggcgtca ccgccactgg cacgcatctg ccttcacggg
     2041 gatttacaac gatttggccg ccgcctcagc ctgtatgtga acacggcagc ggaagccatt
     2101 cgcgccctgt cgatgcagat gccgggcttt cgccgtcaga tgaacgaagg ctggtaccag
     2161 atacgtattg ccggttatga cacggcaccg gaggcggtgt acgcccgtct tcacgaacag
     2221 ctgggtgagg gaacggtcat ccacattgtg ccgcgactgg ccggggccgg aaagggtgga
     2281 ctgcagattg tgctgggggc ggcagccatc gtgggctctt tcttcactgc cggggcatca
     2341 atggcgttat ggggttcagc cctggcagcc ggtggttttt ctgccaccac gatgctgttt
     2401 tcacttggag ccagcatgat tctgggcggt gtggcccaga tgctggcccc gaaggcaaaa
     2461 acaccggatt accgcgcaac ggataacggc agacagaaca cgtacttttc ctcactggat
     2521 aacatgattg cccaggggaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc
     2581 cgccgtatat cccaggacat cagcacccgt gatgaaggcg ggggcggaaa ggtcgtggtt
     2641 atcgggcggc aggggtaaaa agaataaaaa aatcccgcag tgatcgcgga caggaactgc
     2701 gggagagtta cgaagattaa gtgtaaggaa ttattcttat atcacgacaa aaaaattaac
     2761 gcagagaaat tatacgcgcc acagtcagtt tgtgaaaatg tgaagatatt cagaattttt
     2821 atgccattac cggttttaac caacaggatt atcggtgggc atgaaagaaa accccggtat
     2881 ctgctgatac cggggtttct ctttagcatg gcagaaatgt gtttcatgct tttcgggcga
     2941 aggatatccg acttctgtac ggaatggcaa gtggcggtta atttattcag gggaaggctg
     3001 tatgggaaaa ggtggcggta aggcacacac gcctcgtgag gcgaaggata atctcaaatc
     3061 cacgcagatg atgagcgtga ttgatgcgat tggtgaggga ctgatagaag gtccggtgaa
     3121 gggactgcag agtatcctgg tgaacaaaac cccgctgacg gacacggacg gtaatcccgt
     3181 gatacacggt gtgacggcgg tctgacgcgc cggggagcag gagcagacac caccggaagg
     3241 ctttgagtcc tccggtgctg aaaccggact gggcgtggaa gtgacgaagg caaaaccggt
     3301 gacgcgcacc attacgtccg cgaacattga ccgcctgcgg gttaccttcg gggtgcagtc
     3361 actggtgcag accacgtcaa agggtgaccg aaacccgaca tccgtccgcc tgctgattca
     3421 gttacagcgt aacggtaact gggtgacaga aaaggatgtc accattaacg gcaagaccac
     3481 ctcgcagttt ctggcgtcgg tgattctgga taatctgcct ccccgtcctt ttaacatccg
     3541 gatggtccgg gagacagcgg acagcaccac ggaccagctg cagaataaga cgctgtggtc
     3601 gtcatacacc gaaatcatcg atgtgaaaca gtgctacccg aacacggcca ttgtggggct
     3661 gcaggtggat gcggagcagt tcggcggcca gcagatgacg gtgaactacc atatccgcgg
     3721 tcgcatcatc caggtgccgt caaactatga cccggaaaaa cgcacgtaca gtggtatctg
     3781 ggacggtagc ctgaaaccgg catacagcaa caatccggcc tggtgtctgt gggacatgct
     3841 gactcacccg cgctacggca tgggaaaacg tctgggggcg gcggatgtgg acaagtgggc
     3901 gctgtatgcc atcgggcagt actgcgacca gacggtcccg gatagtttcg gggagaccga
     3961 gccgcggatg accttcaatg cgtacctggc acaacagcgt aaggcatggg acgtgctcag
     4021 tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac ggccagacgc tgacgtttgt
     4081 tcaggaccgc ccgtcggatg tggtgtggcc gtacaccaac agcgatgtgg tggtggatga
     4141 taacggcgtg ggtttccgct acagcttcag tgccctgaag gaccggcaca cggcggtgga
     4201 ggtgaattat accgacccgc agaacggctg gcagacctcc acggaactgg tggaagaccc
     4261 ggaagccata ctgcgctacg gacgcaacct gctgaagatg gacgcgttcg gctgtaccag
     4321 ccgcggtcag gcccaccgtg ccggactgtg ggtgataaag accgaactgc tggaaacgca
     4381 gacggtggat ttcacgctcg ggtctcaggg gctgcggcac acacccggtg acatcattga
     4441 aatctgtgat aacgactatg ccgggaccct gaccggcgga cgtgtcctgt ccattgatgc
     4501 tgccacccgc actctgacgc tggaccgtga agtgacactt ccggagaccg gtgccgccac
     4561 ggtgaacctg attaacggca gcggtaagcc ggtgagtgtg gacatcaccg aacaccccgc
     4621 gccggaccgg atacaggtca gtaccctgcc tgatggtgtg gagacatacg gggtgtgggg
     4681 actctccctg ccgtcactgc gccgtctcct gttccgctgt gtctccgtcc gggaaaacac
     4741 ggacggcacc tttgccatca cggcggtgca gcacgtactg gaaaaagaag ccatcgtgga
     4801 taacggtgcc cgctttgagc cgcagtcagg ttccctgaac agcgtcatcc caccggcagt
     4861 gcagcacctg acggccagta tctggcgcag gcgaaatggg acacgccgcg ggtggtgaag
     4921 ggtgtgcgct tcagtctgcg cctgaccagt ggtaagggaa cggatgccag actggtgacc
     4981 accgccatca ccgcagacac ggagcaccgt ttcagcggcc tgccgctcgg ggaatacacc
     5041 ctgacggtgc gggcgataaa cagctatggc cagcagggtg aacctgccac caccaccttc
     5101 cggattgccg caccggcagc accgtcgcgg attgagctga cgccgggcta ttttcagata
     5161 accgcaacgc cgcatcttgc cgtttatgac ccgacggtac agtttgagtt ctggttctcg
     5221 gaaaagcgga ttgcggatat caggcaggtt gaaaccagcg cccgctatct tggcacggcg
     5281 ctgtactgga tagccgccag tatcaatatc aaaccgggcc atgattatta tttttacgtt
     5341 cgcagtgtga acaccgttgg caaatcggca ttcgtggagg ctgtcggtcg ggcgagcgat
     5401 gatgcggaag gttacttgga ttttttcaaa ggagagatag ggaaaacaca tctggctcag
     5461 gagctgtgga cgcagattga taacggtcag cttgcgcctg acctggctga aatcaggacg
     5521 tccattacgg atgtcagcaa tgaaatcacg cagactgtca ataagaaact gaaagaccag
     5581 agtgcggcaa tccagcagat acagaaggtt caggttgata caaataataa tctgaacagc
     5641 atgtgggctg tgaagctgca acagatgaag gacggacgcc tttatattgc gggtatcggt
     5701 gccggtattg agaatacgcc agcaggaatg cagagtcagg tgctgctggc ggcagacagg
     5761 attgcgatga ttaatcctgc gaatggcaac acaaagccga tgtttgttgg tcagggcgat
     5821 cagatattca tgaacgacgt gttcctgaaa cgcctgacgg ctcccaccat taccagcggc
     5881 ggtaatcctc cggcattttc cctgacaccg gacggaaaac tgaccgctaa aaatgcagat
     5941 atcagtggca gtgtgaatgc gaacgccggg acgctcaaca atgtcacaat taatgagaac
     6001 tgtcagatta aggggaaact gtcagccaac cagattgaag gcgatatagt caaaacagtg
     6061 ggtaaggctt ttccgcggga ctcccgggca ccggagcggt ggccatcagg gaccattacc
     6121 gtcaggattt atgacgatca gccttttgac aggcaaattg ttattccggc ggtggcattc
     6181 agcggcgcta aacatgagcg ggagaataac gatatttatt cgtcatgccg cctgatagta
     6241 cggaaaaacg gtgctgaaat ttataaccgt accgcgctgg ataatacgct gatttacagt
     6301 ggtgttattg atatgccagc tggtcgcggc cacatgacgc tggagttttc ggtgtcagca
     6361 tggctggtga ataactggta tcccacagca agtatcagcg atttgctggt tgtggtgatg
     6421 aagaaagcca ccgcaggcat cagtatcagc tgaattttat aacccatata cgggcgccag
     6481 aaatggcgcc ttttttattg cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc
     6541 cattttgtcc gcagccatct gtctgtccgt atccggtgcg cctgcatggg cgtctgaaca
     6601 tcagtccaca ctgagcgcgg ggtatcttca tgcccgtacg aacgctcccg gcagcgataa
     6661 tctgaacggg attaacgtga aataccgtta tgagtttacg gacgcgctgg ggctgattac
     6721 gtccttcagt tatgccaatg ctgaggatga gcaaaaaacg cactacagcg atacccgctg
     6781 gcatgaagat tccgtgcgta accgctggtt cagcgtgatg gcggggccgt ctgtacgcgt
     6841 gaatgaatgg ttcagcgcgt atgcgatggt gggtgtggct tacagccgtg tgtcgacttt
     6901 ctccggggat tatctccgcg taactgacaa caaggggaaa acgcacgatg tgctgaccgg
     6961 aagtgatgac ggtcgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa
     7021 cccgaccgaa tccgtgacca ttgaccttgc ttatgaaggt tccggtagtg gtgactggcg
     7081 aacggatgca tttattgttg gtatcggata ccgtatctga caacagacgc cgatttatct
     7141 tctgtaaata ttgttatgat acgcaggttc atccacctta tggggtgaac tgcgtttgag
     7201 gaaacgtaaa gttacactgt cctgaagccc gtggcgtcac tgctgcgggc tttttttatt
     7261 ggtggaaaag tatgacagta aaaatttctg gcgtgcttaa agatggcaca ggaaaaccag
     7321 tacagaactg caccattgtg ctgaaggcca gacgaaccag cagcacgatg gtggtgaaca
     7381 cggtggcctc tgaaaatccg gatgaagccg gacgttacag catggatgtt gagcatggtc
     7441 agtacagcgt caccctgctg gttgaaggtt ttccgccttc acatgccggg accattaccg
     7501 tctatgaagg ttccagacca ggtacgctga atgattttct cggtgccatg acggaggatg
     7561 atgtccgacc ggaggcactg cgccgctttg agcagatggt ggaagaggtg tcacgtaacg
     7621 cctccgcggt tgcacagaat acggcagccg cgaaaaaatc agccagcgag gcggcaactc
     7681 atgcaaccga tgctgcagcc tcagcacgtg ccgccagcac gtcagccgga caggccgcgt
     7741 cgtcggctca gtcagcgtct tccagcgcag gaacggcatc gacaaaggcc cgtgaagcag
     7801 caaaaagtgc tgctgcagag tcatcaaaaa gcgcggcagc taccagcgcc agtgccgcga
     7861 aaacgtcaga aacgaatgcc gcagcatcac aaaaatcggc agccacttct gcatccacag
     7921 cgaccacgaa ggcgtcagaa gctgccacct cggcacgggg tgcggcggcc tcaaaagagg
     7981 cagcgaaatc ttcagaaacg aatgcatcat caagtgccag tagtgcagct tcctcggcaa
     8041 cggcggcagg aaattccgcg aaggcggcaa aaacgtccga gacgaacgct aagtcttctg
     8101 aaacagcagc gggacagagc gcctcagctg cggcaggttc aaaaacagcg gctgcattat
     8161 ctgccagtgc cgcgtcaaca agtgccgggc aggcctcagc cagtgccacc gccgccggaa
     8221 aatcggcaga aagcgccgca tcatccgctt caacagccac aacgaaggct ggcaaagcca
     8281 ctgagcaagc cactgcagca gcgaggtctg cttctgcagc aaaaacctct gaaacaaatg
     8341 caaagacttc agcagacaat gctgcttcct ctaaggcagc agccgcatcg tcagccagtt
     8401 cagcggcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgacc agacaagcgt
     8461 cagcagcgaa aggtagtgcc acgacagcaa caacgaaagc atcagaggcg gcaggcagtg
     8521 cgacggctgc atctcagagc aaagttgctg ctgaatccgc ggcaacgcgc gccgagacag
     8581 cagcaaaacg ggcagaggat attgcatccg ccgtggcgct ggaggatgcg agcacgacga
     8641 aaaaggggat agtacagctc agcagtgcga ccaacagcac ttccgagtca ctggcggcaa
     8701 cgccaaaagc ggttaaggcg gtaatgggtg aaacgaacaa gaaagcgccc ttaaatagtc
     8761 ctgcactgac cggaacgcca acaacaccaa ctgcgcgaca gggaacgaat aatacccaaa
     8821 tcgcaagcac ggctttcgtt atggctgcga ttgccgccct tgtagattcg tcacctgatg
     8881 cactgaacac gctgaacgag ctggctgcgg cgctgggcaa cgacccgaat tttgcgacca
     8941 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc caccctgacg gcgctggccg
     9001 gacttactac tgcggcaggc aagtttccgt attttacggg gaatgatgtc gccagcctgg
     9061 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgct gccgttatcg
     9121 aa
//
LOCUS       DAZRVY010000148         9071 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZRVY010000148 DAZRVY010000000
VERSION     DAZRVY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.148"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHXOX_003632"
     CDS             complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHXOX_003632"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR7007003.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHXOX_003633"
     CDS             complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHXOX_003633"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR7007004.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHXOX_003634"
     CDS             complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHXOX_003634"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR7007005.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHXOX_003635"
     CDS             complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHXOX_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR7007006.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHXOX_003636"
     CDS             complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHXOX_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR7007007.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHXOX_003637"
     CDS             complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHXOX_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR7007008.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHXOX_003638"
     CDS             complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHXOX_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR7007009.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIARD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHXOX_003639"
     CDS             8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHXOX_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR7007010.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8342..9039
                     /locus_tag="ACHXOX_003640"
                     /pseudo
     CDS             8342..9039
                     /locus_tag="ACHXOX_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2510 c         2585 g         1989 t
ORIGIN      
        1 actttttaga aaaatcgatg gtcagaaact ggattagcaa ttccgttcca gggttgcttt
       61 tgatttacgt tggcgtctga tcattgattt atcctcaaaa gcccaacctc attggtaatg
      121 aaccagctcc gtgaatgtcc gctctggcac agagcgaaat tttttgatct cccccccctg
      181 aaatctaaac ttagtcatgt cacgtttttg ggtttctaaa attttaactt cgcgtttttc
      241 gttgccgtaa gggttataca gaaatgtccg ttaagcagag ttcaaaattg attgccgtga
      301 tcacgactgg tttgaaagcc gcgcccaagc ctgtacagct ctggtttgcg ttgattatga
      361 acctgtcagc ctaaagcaag cggatggacg atgagtattg gtaatctttc agagtccgga
      421 aaagttcagc cccagtctga acaggcttgc tggcgccagt ccagtttcat tcagtcgtgg
      481 tttggttctt acggcctgtg caatctacct cattaggcac atcggcctgc cagataccgg
      541 ctcggggtgt atttccgctt ccacgctgaa tactgttctc agcaatcctg gggtcatcac
      601 ctcttctggt gtgccttgcg ccataacatg tccgtttgcc attaccacca gttgatcgca
      661 gtaccggcta gcctgattaa ggtcgtgcag cacagcgacc accgttttcc cctgagtccg
      721 gagttcgccc atcaaccgca tcaggtccac ctggtgattg atatcaagat aggtggttgg
      781 ctcatcaagt aatacaacgg gcgtattctg ggccaggacc attgccagaa atgcgcgctg
      841 gcgctgaccg ccggaaagct cggttaaccg acgaacggca agatgattga tccgggtctg
      901 gttcatggcg acattaactc gtgcattgtc ttcagcggag agacgccccc agagtgacag
      961 ccagggatta cgaccatacg aaaccagctc ctggactgtg atcccctctg gcgttaaatg
     1021 gtgctgaggc agcagcgaaa gcctgcgggc caactggcgc gatgagagca tatttatggg
     1081 attatcgccg agaaatacgg tgccagactg cggcattaaa agccgcgaaa aacagtttaa
     1141 cagcgtcgat ttcccgcaac cgttaggacc gatcagggcg gtgatcttcc ccgttggcag
     1201 tgagagtgaa acgtcgttaa gtaccttgtc tgtcccgtaa ttgaccgtca gattttcagt
     1261 tcgtaaagtc atttatcgca ttctcacaag caaccagaca aaccacggcg caccgataat
     1321 ggcggtcagc acgccaaccg ggagctccag tgggggatga ataattctcg ccagcagatc
     1381 ggcaaccacc aacagcaacg cacctgtcag ggccgaaaca ggcagcagtc tgcggtgacg
     1441 tccaccggtg atgctacgca tcatatgcgg caccacgaga ccaataaagc taatcgggcc
     1501 gcaggcggcc acgccggtag atgtcatggc gacagctagt aacaaagccc agaatcgggt
     1561 atagggcacc gacacaccga gcgtggtggc gcgcgcatcg ccgagtgcaa ggaggtcgag
     1621 atcgcggcaa aaactcaggc tcagcggcag aaataaaatc atcagcggga tggcaatctt
     1681 cacaaagctc cagtcacggc cccataagct gccggtcagc cacagcaggg cgttgttcac
     1741 atcctgcggg cgcgagagca tcagataatc cgtcaggctg gcccagcatg cagaaagcgc
     1801 cacgccggtg agtgccagct tcatcggctg gtgggtcttt gccagcatct tcagcaatat
     1861 caaccccgcc atgccgcccg caaaggccag cagcggcagc accatcacgg gcagtgacgg
     1921 cataagaagt agagccccca cagaggccag gctggcggca tggttaacgc cgagaatatc
     1981 cggtgatgcc agagggttgc gcacaatccc ctgtatcagc acgcccgcca cggcgagggc
     2041 tgcaccgaca aacagtgcca gcagcaagcg cggcagtcgg tactccatca atacataata
     2101 atgctcgcgt ccggcctgcc agtcggtcag cagcgcgcgc cacggcacgg ggatcactcc
     2161 catatggagt gataacagcg cacagcccgc cagggcaagg gtgatgaaaa taaccagcgc
     2221 aattttcatc ctcgcctcct cacaagccag acaaagcaag ggctgccaat cagcgccagc
     2281 actgcgcctg cgggcagatc tccggggaag gccagcgcgc gtgcgagtac atctgccagc
     2341 agcatcagcg tggcccccag cagcatgctc actggcagta cgttgcgctg atcgaagcct
     2401 gcccagaagc gcgccagatg tggcaccagc agaccgataa acgccaccgg acctgccacg
     2461 ctgacgcacg caccaaccag aagcagcact aacatattga tgaccaaacg tagcctcgtc
     2521 aggttcactc ccagcgtatg ggcggtgctg tcgctgaggt tgagcaggtt cagttgattc
     2581 gccagcagca acacgacagg gactgcagtg accaccaccg gcaagagctg ccagacatcc
     2641 tgccagcggg cgtgggacac tccgccagcc agccagtaaa agatgccgta agcatgatct
     2701 tcggccagca gcagggtgat gcgggtaagg cccatacaaa aggccgacag cgcgataccc
     2761 gcgaggatca gtttgtttct gtcatgggta tgacgaaatc cgcctcctgc ggtcatgacc
     2821 agcagccagc tcacgccgcc cccgcatgcc gcgatgaacg acagagaata gcctgcaatc
     2881 ggcgtcggac tcagcgcgct ggtaagcgcc atagccagcg ccgcgccgct gttaatgccg
     2941 agcagtgaag gagaggccat tgggttgtgg gtcagggttt gcagcagcgt gcccgcgagc
     3001 gccaggcttg cgccgatcag aacggcgacc aggcttcgtg gcaaacgaag gttttgcacc
     3061 agcgcttctg gtagcgttgg cgtgtgtcca ggcagcaggg cgcgggttgc atctgctccg
     3121 gaaacaggaa tggccgagta gcaaaacaga ctcagccaga aaataataat aagtgctgca
     3181 acgggaagcc cccacagcag caccgggtgt tttatcgcgg tcatttcaca acggtaagcg
     3241 gctgatggtg gaagattttt accgtgtcag cggcaatacg ctctgcagca aaaataccgc
     3301 gcatccgcgc ccaggtgtta ctgtcgaccg aagcaacctg ctgcttctgc gcggcggtta
     3361 acatctgcca gagcggatct tgttgccagc gtttaacaat gctctcttcg cgatagtggg
     3421 caaccagcag ccaggcagga ttgaccgcca gcagttgctc caggccgatg gacggcatgg
     3481 acgcacccgc catcgcagcg ggaacgttca gccccagaga ggccagcacg ctgccggtcc
     3541 aggtctcctg agtatgcagg ttgaattgct gttcgcgtga tgtgccaaag gccacgcgtg
     3601 tccctttggg aagctggctg gcccactgcg ccatcctctc tttatgttgt tccagacgtg
     3661 cctgcatctc tcgcttttta cccaccattt cgccgatgat agccgcagat tgcaaatttt
     3721 cagcgtaggt ttcgttgcgg gacttaagca gcagtaccga cgcgatttgc tgcaaggcga
     3781 tgtaaacccc cgcatggcga ctgctgtcgg caatgatcag gtctggtttc agagcggcaa
     3841 tggcttccag gctcggctgc gcgcgcgttc cgacggactg ccacggtttc aggtgcgcac
     3901 gcacttcggg caggatgcgt tttgcatcgt tatcgtcggc aataccgatc gggctgacgt
     3961 ccacggcggc cagcgcatcg gcgaacgaga gttccagcac cacaatccgt tgtggcgttt
     4021 tttcgagtgt aaacgtgccg tgttcgtcct gaaccgtggc ggcaaaggcg tggctgatca
     4081 ccagcagcag gcctgcaaaa agaaaacgga taaatgccaa cataatcaca ttccagctaa
     4141 aagcccggca agccgggcgt taacacatca gaacttcaac gccccctgca tatacagcgt
     4201 gcgcggctgg cctgcataga tgcctttgtt gttgtcgtca taagagcgga tgaagtagtc
     4261 ctggtcgaag atgtttttca caccgaatgc caggttcaga tctgccatct gcgggccaaa
     4321 gtcatacacc acgcgtgcgc cccagagcat gaagccggga atgcggccgg tactgccgtc
     4381 ggcgctctct ttcaccgtat tggcgttatc cgcaaactgg ctggactgga aatcgctgtt
     4441 cagattgaac atccagtttc ctggcttgta gtccacgccc agcgtgcctt tatgtttcgg
     4501 ggagaatggt accagattgc cgtaggtgtc gcctttctcg cggatttccg cgttcacata
     4561 cgcatagctg gcgtagatgg aaacgttatc aagcgttggc gttagcgtac ccagatcgta
     4621 acgtgcctgc gtttccagcc cggtatggcg cgttttgcca cgtgcagtga cggtgtcgtt
     4681 ggtctggttg gagtcgtact gattgttaaa gttaatcagg aacagcccca tttccgccgt
     4741 cagcgcgccg tcgtcgtagc gggtaccgag ttcccaggtt cgcgcttttt ccggttccac
     4801 attgccgctt tgcacagcct tgccaatctg gctgtactgt acggtgccga acgagccttc
     4861 agtgtttgca taaagattcc agctgtcagt caggtgatag agcacgttca acgccggaag
     4921 cggtgcgtta tagctcactt cttcgtgcgt gcctgtgatg gcgttgttct ggtatgactc
     4981 gatatgttcg aaacgcatac ccggcgtgat ggtccagttg ccgatgtcga ttttgtcatc
     5041 cagataccag gcgtgcgcct cggtgccgga acgcgtatcg cggtcgtaag ggcttgagcc
     5101 ggacggcaac tgcccgctgc tggtggcggt gtagtaacgc atttcatgcg ttgattcatt
     5161 cacatagcga tagcccacgc ccacttcgtg cgcggaaggg ccgatcataa agatctggct
     5221 gtagcgtggc tcaataccgc gcacccagta gttacgcggc gagagggtga tgcgtttgcc
     5281 ttgctccagg tagccgctgc gcagggtttg ggtgtagaac ccctgaatgt tgaatttatg
     5341 ctggctgtct ggctggaact ggtagcccag gctcgccagc ttgcgacgac cccagaagcg
     5401 gtcatacggg cgggtggatt gccagcgatc ggcgtcgtaa tccgcgcgag acaggccacc
     5461 gggcatgtcg gcttcaccgt cgtaatattg cagcaggctg ttgaaggtgt gcacctcatc
     5521 cggcgcatat ttgcttttca gcatcaggtc gtcgatgcgg gtggcgctgt gctcgcgcca
     5581 gtcactgccg cgcgtgccgg agtagagcag cgcggtgcca aaaccgttgt ccgctgtgcc
     5641 gcccaccatc aggttgtgcg tctctttcgg gttgttttgt gaagaggttg ggctgagctg
     5701 accttccacg cccgcctcga taccaaagtc ctgcggaatg gcacgggtaa caaagttcac
     5761 cacgccgccc acgctctgcg gtccgtaacg caccgcacca ccaccgcgta ccacgtcaat
     5821 ggcatccatg ttgccgagcg aaacgggagc cagtgaaagc tgcggctgac cgtaaggggc
     5881 gaaggggacg gggatgccgt ccatcaggac ggtcgagcgg ctggcgaggc gcgggttcag
     5941 gccccggatg ccaaagttca tcgccaggtc gtggctgccg gtgccgttgt tttccggcgc
     6001 gctgacgcca gggatgcggt taagtacctc acgcatggtg gttgcgccgg ttttggcgaa
     6061 atcctcacgg cggatcacgt cacgcgcgcc agcatgttca aatacgtcgt tttcacgcgc
     6121 atcacccagc cagtcgccga ccacggtcag ggcatcttct tttggtgcgg gcgcgggctc
     6181 cagcgtccag ctgttatttc ccagcggttt tacctgcagt ccgctgccgt ccagcagttg
     6241 ttgcaggccg ctctcgacgt cgtaatcgcc gtgcaggccg ttgctctgct tgccgcgcgt
     6301 caggctggcg tcaaccgaga gggtaaatcc gctgtgtgcg gcatactgat tgagcgcttt
     6361 atcgagcgat cccggtgcga tattaacctg tgcagcaaaa gcggaaaacg agagaccggc
     6421 cagcggcagc aggctcaggc gaatggcgtt aaccaaaggt gttgttttac gaaaaacgcg
     6481 taacggcgtc ataccttccc catcatcatt tttgttgtgt tcagctatga gtcgaacgag
     6541 aagggtaaaa aggacaatcg aaataagaat tattttcctt acagtggtga aatgtttatc
     6601 cagtaccgcg taatagactg aattttaacg ggaagcgttt gcgcgataac gttcaggatc
     6661 gcatcggtat ttttcagcgg gaacgtcccg ctcaggcgca gcccggcaac ggcgggatcg
     6721 cagcgcagca cgccgttgcg gtaacgggtt agcgtggcta tcacctcacc cagcggttta
     6781 tcgctgaagc tcaggatgtc cttcgtccag cttgtactct cgtcatccag cggtttcact
     6841 gcgccaaact cagaggcgct gaactgcagg ctttcaccag cgttcacgat gcgtttttgc
     6901 gcgggggcac tggcgagaag cacttccacg gcgtgctgct gcacgtcaag ctgcgtgaaa
     6961 ttatcctgct ggcggacggt aaattctgtc cctaaagcag tgagctggcc ctgacgggtc
     7021 aggacacgga aggggcgttg cagggcatct ttcgcggtag taatggcgat ttcaccgtac
     7081 cagagccgga cggtgcgctg atgcgcatca aaacgcatat ccgccgcgct ttgggtattc
     7141 agcgtgagca gggagccatc ttccagttgc tgacggctga ctgtgccttt ggcggtgcgg
     7201 taatctgccc gcagaccttc gccggtttcc gactgccaga gctgccagcc tccgccagcg
     7261 ccgagcaaca gcagtaatcc tttcatcacg tgacggcggg tgaggcgggt atcgtgcaac
     7321 gcccggctgg caacgtcgcc aggcacaccg ccaagctggt tgcgcaggtt ttcaacctgc
     7381 tgccaggccc actggttatc ctgatcctgt tcataccact gttgccagcg cgcttcctgt
     7441 tgtgggctga cgcgctcgcc gcttagcacg gcataccagt gggaagctga acgcagcgcc
     7501 tgacggcggg aatcggttaa caaaggattc ataacccata ctccagacgg aacagcaggc
     7561 agtgctcgac ggctttcgcc acgtattttt tcacggagct gatggaaaca ccgagtttgt
     7621 gcgcaatctc gctgtatgtc agaccatcca gttgcgaaag cagaaacgct tcacgtgttt
     7681 tgccgtttag cccgtccagc atgctgtcga ggagttgtag ggtctcgagt tggctttcgc
     7741 gttcctcagg tgaaggcgct cccccctccg gcataagcgc cagcatctcc agatacgctt
     7801 tttccagcgc gtttcggcga aacaggtcca ccatcacgcg tttggcgata gtgcagagga
     7861 aggagcgagg atcgcggatc gtcgagagcg tttcgctgac cattacccgc aaaaaagtgt
     7921 cccgggcaat gtcatctgca tcaaaagcag actggagttt gcgcgtcagc cagcttttca
     7981 accagccgtg atgtgtgcca taaagcgact cgaatgttaa ggaagctgtg gtagtggcgc
     8041 ggtcagacat gcggagtgca tcaaaagtta attatcacgt agccatatta atatgagaat
     8101 agttatcatt acaattggaa ataaaattgt ttccaataga catttttaac atgttgtttt
     8161 tctaagtgtt ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg
     8221 tcagaatgac tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatagg
     8281 gatttcggta atgctgtcaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
     8341 cgtggcttcc atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa
     8401 cggcaaaagc actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca
     8461 actacagttc acttacaccg cctctcagcc cggtaagcac cagaaaatca ttgatatggc
     8521 catgaatggc gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt
     8581 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt
     8641 gatgtgattg tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt
     8701 cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc
     8761 ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc ctttgaggtc
     8821 gtggtataga tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagctgcac
     8881 gttatcagca agcgttacac tcagcgcatt gagcgacata atctgaatct gagacaacat
     8941 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gcatgacaag
     9001 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctcctcc
     9061 cgaactcaaa a
//
LOCUS       DAZRVY010000149         8973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZRVY010000149 DAZRVY010000000
VERSION     DAZRVY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.149"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1181
                     /locus_tag="ACHXOX_003641"
     CDS             <1..1181
                     /locus_tag="ACHXOX_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7007011.1"
                     /translation="DNTGAEPADEADREQPEETAETVSPVQRLAQLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIMSRVTRAENGARML
                     ESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="vasI"
                     /locus_tag="ACHXOX_003642"
     CDS             1178..1921
                     /gene="vasI"
                     /locus_tag="ACHXOX_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR7007012.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="ACHXOX_003643"
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="ACHXOX_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR7007013.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            3447..6870
                     /gene="tssM"
                     /locus_tag="ACHXOX_003644"
                     /pseudo
     CDS             3447..6870
                     /gene="tssM"
                     /locus_tag="ACHXOX_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            6881..8330
                     /locus_tag="ACHXOX_003645"
                     /pseudo
     CDS             6881..8330
                     /locus_tag="ACHXOX_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            8257..8739
                     /locus_tag="ACHXOX_003646"
     CDS             8257..8739
                     /locus_tag="ACHXOX_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR7007014.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8783..>8973
                     /locus_tag="ACHXOX_003647"
     CDS             8783..>8973
                     /locus_tag="ACHXOX_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007015.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVA"
BASE COUNT         2126 a         2478 c         2547 g         1822 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aggccgacag agaacaaccg gaagagaccg
       61 ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
      361 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
      481 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtatc
      841 tgccgctgtc gaaagagacg ctcgccacca ttatcgccgg aaaactggcc cgtctggata
      901 acgtgctgcg cagtcgcttt ggtgtagaag tggtcattga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgatg
     1021 gcgacatgct accgccgctc tcgctgctgc tgttgcagaa aatggcggct aacacggcga
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
     1501 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cctttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgggg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctaagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcagggcaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatcgtca
     2581 tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 ctggcaacgg cggcgtgggg gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag
     3601 cgcctgcaac agcttgaaaa aatgcaaaag caacagcgcg aggaagccgt tgatccgctc
     3661 agcgtggaac tgaacgccca gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc
     3721 catctcgaca accgccgttt cctgtggcag ttgccgtggt atatggtcat cggcccggcg
     3781 ggcagtggca aaacgacact gctgcgcaag gggtttccgt ccgacattat ttatgccccg
     3841 gagggcgcac gcggcgcaga acaacgcctg tacctcacgc cccatgtcgg taaacaggcg
     3901 gtgatctttg atatcgacgg cacactctgc gctcccgctg atgcggatat cctgcatcgc
     3961 cgtctgtggg aacatgccct cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac
     4021 gggattattc tgacactcga tttacccgat ctgctgaccg cagacaaacg ccgccgcgag
     4081 catctgttac agacgctgcg cagccgctta caggatatac gccagcatct tcactgccag
     4141 ttaccggttt acgtggtact gacccggctt gatttattac agggttttgc cgccctgttc
     4201 cagtcactca acagacagga tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc
     4261 catgaaaatg atgactggcg aacagagttg aatgctttct ggcagacatg ggtggatcga
     4321 atgaatctgg cgttgccgga tctgatggtc gctcagactc acacccgcgc gtctttattc
     4381 agtttttccc gccagatgca gggaagccgt gaaccgctgg tgtcactgct tgagggtctg
     4441 cttgatggcg aaaatatgaa cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag
     4501 cgtggacaga tggatgatat attcacccag tctgccgccc gccagtaccg gctgggcaat
     4561 aacccactgg cgtcctggcc cctggtggac accgcgcctt atttcacccg cagcctgttc
     4621 ccgcaggcat tactcgcaga gcctaatctg gcaacagaga gccgcgcctg gctgatacgt
     4681 tcccgtcgcc gcctgacggt tttctctgcc acaggcggcg tggcagcact gctgctcatc
     4741 accggctggc atcactatta caacggtaac tatcagtccg gcatcaccgt gcttaagcag
     4801 gccaaagcct ttatggacgt gccgcctccg cagggggaag atgactttgg caacctgcaa
     4861 ctgccgctcc tgaacccggt acgcgatgcc acactggcct atggcgactg gggcgaccgc
     4921 agccgtctgg ccgatatggg actgtaccag ggacgacgta tcgggcctta tgtggaacag
     4981 acctatctgc aactgctgga gcaacgttac ctgccctcgc tgtttaacgg gctggtcaaa
     5041 gcgatgaacg ccgcgccgcc ggagagtgaa gaaaaactcg cggtgctgcg cgtgatgcga
     5101 atgctggagg acaaaagcgg acgtaacaat caggtggtga agcagtatat ggcaaaacgc
     5161 tggagcgaaa agttccacgg tcagcgcgat atccaggcac aactgatgtc ccatcttgac
     5221 tacgcgctgg ctcatactga ctggcacgca gagcgtcagg cgggcgacgg tgacgccatc
     5281 agccgctgga cgccatatga caagcccgtg gtatcagcac agaaagaact gagcaaactg
     5341 cctgtctacc agcgggttta ccagagcctg aaaacgcggg cgctgggcgt tcttcctgcc
     5401 gacctcaatc tgcgtgacca ggtagggcca acctttgacc aggtgtttac atctgccgat
     5461 gacaacaaac tggttgttcc acagtttatc acccgctacg gcctgcaaag ctattttgta
     5521 aaacagcgcg atgaactggt tgaactgaca gcgatggatt cctgggtact taacctcacc
     5581 cgcaatgtga aatacagtga cgccgaccgt gcggaaatcc agcaccagtt gaccgagcag
     5641 tatatcagcg actacaccgc cacctggcgg gctgggatgg acaatctgaa tatccgcaat
     5701 tttgagtcca tcggacaact gaccggggcg ctggagcagg ttatcagcgg cgaccagcct
     5761 ttgcagcggg cgctgaccgt gctgcgtgac agcacacagc caggcgtctt ttctgaaaaa
     5821 ctctctgcca aagaacggga ggaagccctg gcagagccgg attaccagtt actcacccgc
     5881 ctcgggcatg aattcgcccc ggaaaacagt accctggcag tacagaaaga caaagaaagc
     5941 acgatgcagg ccgtgtatca gcaactcacc gagttgcacc gctacctgct ggcaatccag
     6001 aacgcgcctg taccagggaa atcggcgctg aaagccgtgc agttacggct tgatcagaac
     6061 agcagcgatc cgatattcgc cacccgccag atggcaaaaa cgctgcctgc tccgctcaac
     6121 cgctgggttg gcagactgac tgactaggcc tggcatgtgg tgatggtgga ggctgttcat
     6181 tatatggaag tggactggcg cgacagcgtg gtgaaaccgt ttaacgagca actggcaaat
     6241 aactatccgt ttaatccgcg ttctgcacag gatgcctcac tggatgcctt cgaacgcttc
     6301 tttaaaccgg atggcatact ggataccttc taccagcaga acctgaagct gtttatcgat
     6361 aatgacctga gtctggagga tggcgataac aacgtcatta ttcgcgaaga tattattgcg
     6421 caactggaaa ctgcgcagaa aatccgtgac atcttcttca gcaaacagaa cggtctggga
     6481 acatcctttg ccgtggaaac ggtatcgctt tcaggcaata aacgccgcag tgtactgaac
     6541 cttgacagtc agttagtcga ttacagccag ggccgtaact ataccgccca tctggtctgg
     6601 cctaacaaca tgcgcgaagg caacgaaagt aagctgacgc tcatcggcac cagcggcaac
     6661 gcgccgcgca gtatcagctt cagcgggccg tgggcgcagt tccgcctgtt ctgggccgga
     6721 caactgaccg gagtacagga tggcaacttt accgtgcgct ttagcgtgga cggtggcgcg
     6781 atgacctacc gtgtgcatac cgacacggaa gataacccgt tcagcggtgg gttgttcagc
     6841 cagtttggtc tgtcagacac actgtactga ggaggcaggg atgaatagta acgtactgac
     6901 acaaactatc gttaccggca gtgacccgcg cgggctgccg gaattcagcg ccatccgcga
     6961 ggaaataaac aaagccagcc acccgtcaca gcctgagctg aactggaaac tggtggagtc
     7021 gctggcgctg gcgattttta aagccaacgg tgtggattta cacaccgcca cctactatac
     7081 gcttgcccgg acacggacac agggactggc gggattctgc gaaggtgcgg aactgctggc
     7141 ggcaatggta agccacgact gggataagtt ctggccgcag ggcggcccgg cgcgtactga
     7201 aatgctggac tggtttaact cccgaaccgg caatattctg cgtcagcaaa tctcctttgc
     7261 ggaatccgac ctaccactga tctaccgcac agagcgggca ttgcagctta tctgcgataa
     7321 gctccagcag gtggaactga agcgcgttcc gcgcgtggag aatctgctct attttatgca
     7381 gcacacgcgt aaacggcttg aaccccagcc gaagagtaac gctgagaacg ccgcacagac
     7441 cacggtcaga acgctgattt atgccccgga aacactggca tcttccacac cagaagcggt
     7501 agtgcctccc ctgcccggcc tgcctgagat gaaagtggaa gtgcgcagtc tgacagagaa
     7561 tcccccacag gccagtgtga taaagcaagg cagtacggta agagggttta tcgcagggat
     7621 cgcctgttca gtggctgtcg cctcagcatt gtggtggtgg caggtctatc cggtgcagca
     7681 gcaactgtta caggttaacg acaccgctca gggcgcagca acggtgtgga tggcctcacc
     7741 tgaactcgaa aactatgagc gcaggctgca acaacttctt gatacctccc cggtacagcc
     7801 gctggaaacc gggatgcaga tgatgcgtgt tgccgacagt cgctggccgg aaagcctgca
     7861 acagcaacag gcctcgacac aatggaatga ggcactcaaa acccgcgcac agagtagccc
     7921 gcagttgcgt ggctggttgc agacccgcca ggacttacat gcttttgcag atctagtgat
     7981 gcagcgcgag aaagagggac taaccctttc ctatatcaaa aatgtcatct ggcaggcgga
     8041 gcggggactg gggcaggaaa cccccgttga gtctctgttg acgcagtacc atgatgcccg
     8101 tgcgcagaag cagaatacag atgcgctgga aaaacaaatt aatgagcgac tcgaaggcgt
     8161 gttaagccgc tggctgctgc tgaagaataa cgtcatgcca gaggcggcaa ccggtaccac
     8221 ggccgaaaaa taattattta attaaaggag attatcatgg caaatataag ttatttatct
     8281 ttgagtggag agacacaagg gctaatttca gcaggttgct caacgcttga ttctgtcgga
     8341 aacaaagcgc agccagaaca taaagatcag atcatggttt atgcgttaat gcattccatc
     8401 tcaagaagtc aaaatgtcaa tcatcatgaa ttaattatca ccaaaccggt tgataaatcc
     8461 tcaccattgc tggcaaaagc acttagtgac aatgaaaaaa tggcaatatg tgaatttata
     8521 ctctaccgaa ccagtaaagc gggtatttac cagccttatt ataaaataaa tctgtcaaaa
     8581 gcccgaatat caagtattga ttttgtcaca ccgcatgctg tcctggaaaa agaactggaa
     8641 ccacaggaga gaattgcctt tatttatgaa gatatttcct gggagcatac gttagccggt
     8701 acgaatgcaa tgagtaaatg gcaggatcgt gtacagtaac ttactgtaat aacataataa
     8761 attactattg cggagagata aaatggctgg tttaaggaat aacaacaaca cacagaatgc
     8821 acagtgggct gattatgtgg gtgacattct tcggggggct cagccgataa accagctagt
     8881 acctcagcac ccttatctta acgacgtccc actcattgat gagttaaggc atcagaacac
     8941 tcatcatgtt attctcttga cacttgatgt tgc
//
LOCUS       DAZRVY010000150         8900 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZRVY010000150 DAZRVY010000000
VERSION     DAZRVY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.150"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(509..772)
                     /gene="rpsT"
                     /locus_tag="ACHXOX_003648"
     CDS             complement(509..772)
                     /gene="rpsT"
                     /locus_tag="ACHXOX_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR7007016.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            875..1093
                     /locus_tag="ACHXOX_003649"
     CDS             875..1093
                     /locus_tag="ACHXOX_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR7007017.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1101..2042
                     /gene="ribF"
                     /locus_tag="ACHXOX_003650"
     CDS             1101..2042
                     /gene="ribF"
                     /locus_tag="ACHXOX_003650"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR7007018.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2085..4901
                     /gene="ileS"
                     /locus_tag="ACHXOX_003651"
     CDS             2085..4901
                     /gene="ileS"
                     /locus_tag="ACHXOX_003651"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR7007019.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4901..5395
                     /gene="lspA"
                     /locus_tag="ACHXOX_003652"
     CDS             4901..5395
                     /gene="lspA"
                     /locus_tag="ACHXOX_003652"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR7007020.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5520..5969
                     /gene="fkpB"
                     /locus_tag="ACHXOX_003653"
     CDS             5520..5969
                     /gene="fkpB"
                     /locus_tag="ACHXOX_003653"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7007021.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5971..6921
                     /gene="ispH"
                     /locus_tag="ACHXOX_003654"
                     /gene_synonym="lytB"
     CDS             5971..6921
                     /gene="ispH"
                     /locus_tag="ACHXOX_003654"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR7007022.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6987..7901
                     /gene="rihC"
                     /locus_tag="ACHXOX_003655"
     CDS             6987..7901
                     /gene="rihC"
                     /locus_tag="ACHXOX_003655"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR7007023.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            8068..8889
                     /gene="dapB"
                     /locus_tag="ACHXOX_003656"
     CDS             8068..8889
                     /gene="dapB"
                     /locus_tag="ACHXOX_003656"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR7007024.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         2061 a         2183 c         2502 g         2154 t
ORIGIN      
        1 cgaagttgag ataccatttg tcattatcag tccttgaagt tgatgaattt ttattaattt
       61 aatttatgag tgaatgtgac tactatcaaa aatagctcat taattttatg aagtagaata
      121 taaataggta tttaacctgt gttgattgtt gttaattatt tacaagatct aataaggaga
      181 tatttatgaa tggttaaagt gtataggaat ttaattcaaa aatagagtgg tcttaagttt
      241 aatacctcaa tgtgtatcac aatatccaca ttctttgtag gggagtctgg agattgagta
      301 gatattcttg ttcagaatgt atcagccgat ggttctacga ttcttaagcc acgaagagtt
      361 cagaaagtgt aacggcatgt ctgcaaaagc cagcatcttt tgactatctg gcaaccggta
      421 gtgtgttctc tcacgcatca taaaagcagc aggcataaaa aaaacccgct tgcgcgggct
      481 ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt gcagtcaggt
      541 tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc tgacggtcca
      601 cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca gcttcgatag
      661 ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg ttgtgcttac
      721 gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc aaggtccaac
      781 tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt ttagccttct
      841 tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt tacgtagtga
      901 tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga taaaaattgc
      961 agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc acttctttct
     1021 gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac tcggacgatt
     1081 ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc agccaggccc
     1141 cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc ggtcatcgcg
     1201 cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg atggtgatgc
     1261 tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca agactgaccc
     1321 ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc
     1381 gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat cttctggtga
     1441 agcatttgcg cgtaaaattt cttgccgtag gggatgattt ccgctttggc gctggtcgtg
     1501 aaggcgattt cttgttatta cagaaagctg gcatggaata cggcttcgat atcactagta
     1561 cgcaaacttt ttgcgaaggt ggcgtgcgta ttagcagcac cgctgtgcgt caggcgctgg
     1621 cggatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc atctccgggc
     1681 gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg aatgtaccgc
     1741 tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg
     1801 aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc
     1861 agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag
     1921 tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac gaactgaaag
     1981 cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca aaaccggctt
     2041 aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt gactataaat
     2101 caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc aagcgcgaac
     2161 cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt gcggctaaaa
     2221 aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc agcattcata
     2281 ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa gggctttccg
     2341 gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc gagctgaaag
     2401 tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc cgcgccaagt
     2461 gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc cgtctgggcg
     2521 tgctgggcga ctggtcgcac ccgtacctga ccatggactt caaaactgaa gccaacatca
     2581 tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg aagccagttc
     2641 actggtgcgt tgactgccgt tctgcgctgg cggaagcgga agttgagtat tacgacaaaa
     2701 cttctccgtc catcgacgtt gcttttcagg cggtcgatca ggatgcactg aaagcaaaat
     2761 ttgccgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact acgccgtgga
     2821 ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg ctggtgcaga
     2881 tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg cagcgtatcg
     2941 gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag ttgctgcgct
     3001 ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac gttaccctag
     3061 atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac tatgtgatcg
     3121 gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact tatctgccgg
     3181 gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc gtcgttgcgc
     3241 tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc tatccgtgct
     3301 gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc gtcagcatgg
     3361 atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag tggatcccgg
     3421 actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg tgtatctccc
     3481 gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg gaagagctgc
     3541 atccgcgtac tctcgaacta atggaagaag tggcaaaacg cgttgaagtt gacggcatcc
     3601 aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga tgaagctgat cagtacgtga
     3661 aagtgccgga cacattggat gtatggtttg actccggatc tacccactct tctgttgttg
     3721 acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt tctgaccagc
     3781 accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc aaagcgccgt
     3841 atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag atgtctaaat
     3901 ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg gatattctgc
     3961 gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac gagatcctga
     4021 aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg ctggcaaacc
     4081 tgaacggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg gtactggatc
     4141 gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg tacgaagcat
     4201 acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag atgggttcct
     4261 tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggacagt gtggcgcgtc
     4321 gtagctgcca gactgcgctg tatcacatcg cagaagcgct ggtgcgctgg atggcaccaa
     4381 tcctctcctt caccgctgat gaagtgtggg gctacctgcc gggcgaacgt gaaaaatacg
     4441 tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc agacagtgaa gcgatgaacg
     4501 atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc attgagcaag
     4561 cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg tatgcagaac
     4621 cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc ctgttgacct
     4681 ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag agcgaagtac
     4741 tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca cgctgctggc
     4801 actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc cgctgtgtca
     4861 gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat cgatctgttc
     4921 aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc tgggcagcaa
     4981 atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa
     5041 tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg
     5101 gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgatcctgg cagtgatgat
     5161 gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga ttattggcgg
     5221 cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata tgatcgactt
     5281 ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca tctgtgtcgg
     5341 tgcggcactg attgtgctgg aaggtttttt gccttctaga gcgaaaaaac aataataaac
     5401 cctgccggat gcgatgctga cgcatcttat ccggcctaca gattgctgcg aaatcgtagg
     5461 ccggataagg cgtttacgcc gcatccggca aaaatcctca aatataagag caaacctgca
     5521 tgtctgaatc tgtacagagc aatagcgccg tcctggtgca cttcacgcta aaactcgacg
     5581 atggcaccac cgccgagtct acccgcaaca acggtaaacc ggcgctgttc cgcctgggtg
     5641 atgcttctct ttctgaaggg ctggagcaac acctgttggg gctgaaagtg ggcgataaaa
     5701 ccaccttctc gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac ctgattcagt
     5761 acttctcccg ccgtgaattt atggatgcag gcgagccaga aattggcgca atcatgcttt
     5821 ttaccgcaat ggatggcagt gagatgcctg gcgtgatccg cgaaattaac ggcgactcca
     5881 ttaccgttga tttcaaccat ccgctggccg ggcagaccgt tcattttgat attgaagtgc
     5941 tggaaatcga tccggcattg gaggcgtaac atgcagatcc tgttggccaa cccgcgtggt
     6001 ttttgtgccg gggtagaccg cgctatcagc attgttgaaa acgcgctggc catttacggc
     6061 gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga tagcttgcgt
     6121 gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt
     6181 ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagccg cgatttgacg
     6241 gtgtttgatg ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagc
     6301 cgtcgtggcg aagagtcgat tctcatcggc cacgccgggc atccggaagt ggaaggtaca
     6361 atgggccagt acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc ggacgatgtg
     6421 tggaaactga cggtcaaaaa cgaagagaag ctctccttta tgacccagac cacgctgtcg
     6481 gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa aattgtcggt
     6541 ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg
     6601 gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt
     6661 ctggcggagc tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagat
     6721 atccaggaag agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct
     6781 ccggatattc tggtgcaaaa tgtggtggca cgtttgcagc agctgggcgg tggtgaagcc
     6841 attccgctgg aaggccgtga agaaaacatt gttttcgaag tgccgaaaga gctgcgtgtc
     6901 gatattcgtg aagtcgatta agtcattagc agcctaagtt atgcgaaaat gccggtcttg
     6961 ttaccggcat tttttatgga gaaaacatgc gtttacctat cttcctcgat actgaccccg
     7021 gcattgacga tgccgtcgcc attgccgccg cgatttttgc ccccgaactc gacctgcaac
     7081 tgatgaccac cgtcgcgggt aatgtctcgg ttgagaaaac cacccgcaac gccctgcaac
     7141 tgctgcattt ctggaatgcg gagattccgc tcgcccaggg agccgctgtg ccgctggtac
     7201 gcgcaccgcg tgatgcagca tctgtgcacg gcgaatcggg aatggctggc tacgactttg
     7261 ttgagcacaa ccgaaagcca atcgggatac cagcgtttct ggcgattcgt gatgccctga
     7321 tgcgtgcacc agagcctgtt accctggtgg ccattggccc gttaaccaat attgcgctgt
     7381 tactttcaca atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc atgggcggtt
     7441 cttctggtcg cggcaactgt acaccaaatg ccgagtttaa tatcgctgcc gatccagaag
     7501 cggctgcctg tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg gatgtcacca
     7561 atcaggcaat attaacccct gactatctcg ctactttgcc ggagttgaat cgcaccggaa
     7621 aaatgcttca tgcactgttt agccactacc gtagcggcag tatgcaaagc ggtctgcgaa
     7681 tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga cttattcacc ctcaagccgt
     7741 gttttgtcgc ggtagaaact caaggcgaat ttacctcagg cacgacggtg gttgatatcg
     7801 acggttgcct gggcaaacca gccaatgtaa aggtggcatt ggatctggat gtgaaaggct
     7861 tccagcagtg ggtggctgag gtgctggctc tggcgtcgta acctgtcaca tgttatcggc
     7921 atgcagtcat tcatcgactc atgcctttca ctgatatccc tccctgttta tcattaattt
     7981 ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc tggttactct gaaaacggtc
     8041 tatgcaaatt aacaaaagag aatagctatg catgatgcaa acatccgcgt tgccatcgcg
     8101 ggagccgggg ggcgtatggg ccgccagttg attcaggcgg cgctggcatt agaaggcgtg
     8161 cagctgggcg cggcgctgga gcgcgaagga tcttctttac tgggcagcga cgccggtgag
     8221 ctggctggtg cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc ggtaaaagat
     8281 gattttgatg tgtttatcga ttttacccgt ccggaaggta cgctgaacca tctcgctttt
     8341 tgtcgccagc atggcaaagg gatggtgatc ggcactacgg ggtttgacga agccggtaaa
     8401 caggcgattc gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa ttttagcgtt
     8461 ggcgttaacg tcatgcttaa gctgctggag aaagcagcca aagtgatggg tgactacacc
     8521 gatatcgaaa ttattgaagc acatcataga cataaagttg atgcgccgtc aggcaccgca
     8581 ctggcaatgg gagaggcgat cgcccacgcc cttgataaag atctgaaaga ttgcgcggtc
     8641 tacagtcgtg aaggccacac cggtgaacgt gtgcctggca ccattggttt tgccaccgtg
     8701 cgtgcaggtg acatcgttgg tgaacatacc gcgatgtttg ccgatattgg cgagcgtctg
     8761 gagatcaccc ataaggcgtc cagccgtatg acatttgcta acggcgcggt aagatcggct
     8821 ttgtggttga gtggtaagga aaatggtctt tttgatatgc gagatgtact taatctcaat
     8881 aatttgtaac cacaaaatat
//
LOCUS       DAZRVY010000151         8879 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZRVY010000151 DAZRVY010000000
VERSION     DAZRVY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.151"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            162..1560
                     /gene="ulaA"
                     /locus_tag="ACHXOX_003657"
                     /pseudo
     CDS             162..1560
                     /gene="ulaA"
                     /locus_tag="ACHXOX_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1576..1881
                     /gene="ulaB"
                     /locus_tag="ACHXOX_003658"
     CDS             1576..1881
                     /gene="ulaB"
                     /locus_tag="ACHXOX_003658"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR7007025.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1891..2352
                     /gene="ulaC"
                     /locus_tag="ACHXOX_003659"
     CDS             1891..2352
                     /gene="ulaC"
                     /locus_tag="ACHXOX_003659"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR7007026.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2366..3016
                     /gene="ulaD"
                     /locus_tag="ACHXOX_003660"
     CDS             2366..3016
                     /gene="ulaD"
                     /locus_tag="ACHXOX_003660"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR7007027.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3026..3880
                     /gene="ulaE"
                     /locus_tag="ACHXOX_003661"
     CDS             3026..3880
                     /gene="ulaE"
                     /locus_tag="ACHXOX_003661"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR7007028.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3880..4566
                     /gene="ulaF"
                     /locus_tag="ACHXOX_003662"
     CDS             3880..4566
                     /gene="ulaF"
                     /locus_tag="ACHXOX_003662"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR7007029.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4663..5214
                     /locus_tag="ACHXOX_003663"
     CDS             4663..5214
                     /locus_tag="ACHXOX_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR7007030.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5289..5564)
                     /gene="yjfY"
                     /locus_tag="ACHXOX_003664"
     CDS             complement(5289..5564)
                     /gene="yjfY"
                     /locus_tag="ACHXOX_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR7007031.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5891..6286
                     /gene="rpsF"
                     /locus_tag="ACHXOX_003665"
     CDS             5891..6286
                     /gene="rpsF"
                     /locus_tag="ACHXOX_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR7007032.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6293..6607
                     /gene="priB"
                     /locus_tag="ACHXOX_003666"
     CDS             6293..6607
                     /gene="priB"
                     /locus_tag="ACHXOX_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR7007033.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6612..6839
                     /gene="rpsR"
                     /locus_tag="ACHXOX_003667"
     CDS             6612..6839
                     /gene="rpsR"
                     /locus_tag="ACHXOX_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR7007034.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6881..7330
                     /gene="rplI"
                     /locus_tag="ACHXOX_003668"
     CDS             6881..7330
                     /gene="rplI"
                     /locus_tag="ACHXOX_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR7007035.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7442..8413)
                     /locus_tag="ACHXOX_003669"
     CDS             complement(7442..8413)
                     /locus_tag="ACHXOX_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR7007036.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8466..>8879)
                     /locus_tag="ACHXOX_003670"
     CDS             complement(8466..>8879)
                     /locus_tag="ACHXOX_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR7007037.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2136 a         2170 c         2359 g         2214 t
ORIGIN      
        1 tttcgttgtg tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta
       61 atcataaata atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg
      121 gtgtgcggaa tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca
      181 tctttaccgt gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga
      241 cctgtctggg ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta
      301 aaaccataat tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca
      361 aaccggtggt ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact
      421 tacgcgtcga tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac
      481 gcggtgttgt tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt
      541 attcgtacca tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt
      601 acgttgttta ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt
      661 tcgctctact ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac
      721 ggctgcggtt tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt
      781 gcgccgttcc tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg
      841 aatattttcc acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc
      901 attctgctct ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg
      961 gtctatatcc tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt
     1021 gtgcgcatgt ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc
     1081 cctggtgcgg ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg
     1141 gtctggggct ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc
     1201 tgcggttcct cgatcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc
     1261 atcggcgtgt tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg
     1321 atggggatga tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc
     1381 tggatgggga tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc
     1441 atcggtatcg cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct
     1501 ggccgcgcac tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa
     1561 taaggagttt tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt
     1621 tccatgatca tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg
     1681 gtaaatagct gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc
     1741 gcttctacgc acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg
     1801 cgcaacatgc tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat
     1861 ttcccgcagg atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat
     1921 aaatccatcc gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt
     1981 gacctgctgg tggcggatgt tgtcgagccg cgttactacc aggcgattct ggatgcggtt
     2041 gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca cggtcgcccg
     2101 gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc gctggagttc
     2161 aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt cgatgccaac
     2221 acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga agaaaatttc
     2281 gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga ccgcaccaac
     2341 gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg cgctggacaa
     2401 ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag tcgacattat
     2461 cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg atctgaaagc
     2521 gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg gcaaaattct
     2581 ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt gtgcggatat
     2641 caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg tgcagatcga
     2701 actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag gtattcagca
     2761 ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg gtgaagccga
     2821 tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca ccggtggcct
     2881 ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta tcgccggtcg
     2941 cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac gttccatcgc
     3001 cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg gcatctatga
     3061 aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa aaacgttggg
     3121 cttcgatttt gtcgagatgt cggtagatga aactgacgag cgcctgtcgc gcctcgactg
     3181 gagccgcgag cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg tgcgtgtgcc
     3241 gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg acgcggtgcg
     3301 ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg ttggcattcg
     3361 cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg aaacgcgtcg
     3421 tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc aggtgacgct
     3481 ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc tgggttacgc
     3541 gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc tgtcggcgtg
     3601 ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg cggtgcatgt
     3661 gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg tggtggattt
     3721 cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc tgattgagat
     3781 gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgtg attgggtgaa
     3841 agcgcgcatg gccaaagcag gcacggtgga ggcggcataa tgcaaaagct aaaacagcag
     3901 gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac ctggggcaac
     3961 gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg cgttgcctac
     4021 gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg
     4081 gagtatcgcc cgtcttccga caccgcgacg catctcgaac tctaccgtcg ttacccgtcg
     4141 cttggcggca ttgtccatac ccactccact catgctactg catgggcgca ggcggggctg
     4201 gcgatcccgg cgttaggcac tacgcacgcc gactacttct ttggcgacat cccgtgtacg
     4261 cgcggattaa gcgaagaaga ggtgcagggc gagtatgaac tgaacaccgg caaagtgatt
     4321 atcgaaacgc tgggtgacgc cgagccgctg catacgccgg ggattgtggt gtatcagcac
     4381 ggtccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt ggtgatggaa
     4441 gaagtggcga aaatggcgtg gattgcgcgc agtattaacc cacaactgaa tcacatcgac
     4501 agcttcctca tgaataaaca cttcatgcgt aagcacggtc ctaacgccta ttacgggcag
     4561 aagtaagcag tgcgccccgg aattattacc tcctgcggga tgtaattggt ctgattccgg
     4621 ggccgcttca gttaaaaaat cgtataaaca acaggtgttt ttatggctat caatggattg
     4681 tttgtcagaa cggatccgca acgtcgccgc tatggcgttg cgttacttgt gggtgtactt
     4741 tcaggaatta tttcagcatt tgttaaatgg ggagcggaag tccccttgcc accgcgcaca
     4801 ttttccggtg gtcgcgatga atttaacccg ccgtatatct ttctacggga ttatttaggt
     4861 atcgacccaa cccaaactgt gtatacattc tcggaacata ttatcaaccc ggtgatggtg
     4921 acgcatatca tcttctcact ggtttttgcg atagggtatt gtattgtcgc ggaaattttc
     4981 ccgaaagtga aattatggca ggggatatta gcagggctta ttgtgacagt ggcagtccac
     5041 ggtatatttt gcccggcgct gaatttaacg ccgccgctca ctcaattgcc ttttgacgaa
     5101 tatgcctctg aaatcctggg gcatattttc tggttctgga taatagaaat tatgcgccgc
     5161 gacttacgta atcgcattac tcatgagcca gatccggaag tcgcaatccg gtgaataacc
     5221 gcataaaaaa gccccctgcg aagggggcaa agcaaactat ggcaatgttt cgttggttat
     5281 acctggtgct agcgataaat atccgcgctg gcgtgcatat tgccgttact ccccggttcc
     5341 cgcatcagaa ttacgtggta gtacgttgcg ccctgcgcat ctgtctcttc atttaatgcc
     5401 tgacgcgctt cgctttcagt ggcgaaatta tgattgatat aaatcacgcc taagctttgc
     5461 acatcatcca tgtttctggc ctggtggtta ttaatttcaa tggctgccca tgtatttgca
     5521 cttagcaaaa gcacagccag aagggctaaa actcgactga acatagatac ctcctcgacg
     5581 gctgactttg tgtgctctcc tctgtgatga tcttctgatt taattttaat caatgataaa
     5641 gaagttgatg gtgaccattt ctgatgcagt tgttcaaaaa aacaccatga tgaagtgtga
     5701 tgaactccaa atcagcggtt tagaggttaa ttgcgaaagg ggagatttat ttcggctctg
     5761 cccttgagtt tagcggggca tacaagtact ataacgcgtc atttttcagc cgacctttaa
     5821 cacgttcctt gcctccccgg gattcggctg acccagacag gaggctgaat aatccgtaag
     5881 gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc catcctgacc agagcgaaca
     5941 ggttccgggc atgatcgagc gctacactgc tgccatcact ggtgcagaag gcaagatcca
     6001 ccgtctggaa gactggggcc gccgtcagct ggcttacccg atcaacaaac tgcacaaagc
     6061 acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc tggaaactac
     6121 cttccgcttc aacgatgccg ttatccgcag catggttatg cgtaccaagc acgctgttac
     6181 cgaagcatct ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg atgatttcgc
     6241 aaacgaaacc gctgatgatg ctgaagctgg ggattctgaa gagtaatttc tgatgaccaa
     6301 ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca gtccatcagg
     6361 aattcctcac tgccagttcg tgcttgagca tcgttctgtg caggaggagg ccggttttca
     6421 ccggcaggcg tggtgccaaa tgcccgttat tgttagcgga cacgaaaacc aggccattac
     6481 tcacagtata acggtcggca gtcgcataac cgttcagggg ttcatttcat gccacaaggc
     6541 aaagaacgga ctgagcaaaa tggttttgca tgccgagcag attgaattga tagattctgg
     6601 agactagcca tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc accgcggaag
     6661 gcgttcaaga gatcgactat aaagatatcg ctacgctgaa aaactacatc accgaaagcg
     6721 gtaagattgt cccaagccgt atcaccggta cccgtgcaaa ataccagcgt cagctggctc
     6781 gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta cactgatcgc catcagtaat
     6841 cggtcacggt ccattaatac gactttgaga ggataaggta atgcaagtta ttctgcttga
     6901 taaagtagca aacctgggta gcctgggtga tcaggtaaac gttaaagcgg gctatgctcg
     6961 taacttcctg gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca ttgaattctt
     7021 cgaagcacgt cgcgctgaac tggaagctaa actggctgaa gttctggcag ctgctaatgc
     7081 tcgcgctgag aaaatcaatg cactggaaac tgttaccatc gcgtctaaag ctggcgacga
     7141 aggtaaactg ttcggttcca tcggtactcg cgacatcgct gacgctgtaa ctgcagctgg
     7201 cgttgaagtg gctaagagcg aagttcgtct gccgaacggc gttctgcgta ccactggcga
     7261 gcacgaagtg agcttccagg ttcacagcga agtattcgcg aaagtgatcg taaacgtagt
     7321 agctgaataa ttcgttattc aacgagacgt aaaaacgccg accagtggtc ggcgttttgc
     7381 ttttgtagcg agcacttgca gatatcgata agggccacaa taagcagccc tacgaagata
     7441 attacttagc tgcctgaata tcagccagca ttggggcaag ggctgggttg tccttagcga
     7501 agtttgcgta cattggctgt actttctcga taaacggagc tttatctacc tggtagactt
     7561 tcacgcccat tttatccagt tttgcaaatt cttgtttctc ttcctcgctc cacaagtctt
     7621 tcatgtagct cattgattct ttagctgctt ttaatataac tgcctgattt tccgggctaa
     7681 gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg aaccatagtg tgttcgtcaa
     7741 ggctgaagga ttttgccact tcaccgtgac gcatggtggt cagcgccatc acgctgtttt
     7801 ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa ctcgccgtaa tccagtggcg
     7861 tcgggttacc gcccatgact ttgatcattt caactgcgcc agggcttggc tggacacgga
     7921 ctttcatgcc tttcagatcg tctggtttca gtactggttt attgccatag aaactgcggg
     7981 cgcctccatc ataaaaagtc agcccgaaat aacctttgct tttcgttgag tcgaggattt
     8041 ttctcccgat atcgccctgc atgacctgat aataatgatc acgatcgcgg aacaaataag
     8101 gcaggctaaa taatttatag gaattttcaa atgcttccat ttctgccgca ttggttttta
     8161 ccagtggaat agcgccagaa cgaatcagct ccattgtttc tcgttgagta cccaatgttc
     8221 catttggata aatacgaata gtaatatcac cgttagagta ctctttgttc tttttcgcaa
     8281 agaactccat cgctttatga acaggatgag acttatcctg attatgactc agttttaatg
     8341 tcactgccca ggactggctt gcggataaca taagaacagc accgataatg ccagcggata
     8401 cttttatttt catagatgtt ccttttctaa taaatatggc ggcaggtgcc gccagtgaat
     8461 gcgcatcagt agcccagtgc ttttggcaat gccatactga tttgcggaat ataggtcacc
     8521 attgccagca ccaataccat cacggcatag aatggcagca taggtttgat aattttatta
     8581 atcggtaatt tagatacact acaaccgaca aataacacac tgcctacagg cggagtacaa
     8641 ataccgatac acatgttcag taccatgaca ataccaaagt gaatcgggtc catgcccagt
     8701 tcggtgacaa caggtaaaaa tatcggcgta aaaatcagta ccgccggtgt catatccata
     8761 aaagtgccga taatcaacag gataatattg ataataagca ggattattat tgggttgtca
     8821 gaaatagtgt taagcaaatc gagaatcagg aagggaacgt cagcgtttga cattgccca
//
LOCUS       DAZRVY010000152         8821 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZRVY010000152 DAZRVY010000000
VERSION     DAZRVY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.152"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            69..1547
                     /locus_tag="ACHXOX_003671"
                     /pseudo
     CDS             69..1547
                     /locus_tag="ACHXOX_003671"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            1559..2089
                     /locus_tag="ACHXOX_003672"
     CDS             1559..2089
                     /locus_tag="ACHXOX_003672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007038.1"
                     /translation="MTLLPEKIYMVSNPMDLTVLVPAATILVISFTSSGKSTSLDNLN
                     GSTSKEFTVVLDMSTAQELLFCTQISNGQTSTFTSEVINPLLYTSEYSQLKQMISEID
                     QVIANKIAGGANYSITINNKTLVSESLSSLESMRERYVKRANALFVKMNGGTFSNGGK
                     PIKSITVFRPKPGSTR"
     gene            2089..3597
                     /locus_tag="ACHXOX_003673"
     CDS             2089..3597
                     /locus_tag="ACHXOX_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7007039.1"
                     /translation="MFWNKKNKNSETKPKKIKQERSLKQSTLKRDLQAVRNTSVMNFG
                     FNSNSGSNINFLLMKALPTFRAFSRDAVLKNPIGRKYMNLSVDGVVGSDGLYVKPSVE
                     IDGTEEEINGINLTLEKLFDRWAYDADRFSVDGSLSFDLFQQNAEKVRVQDGECFIRI
                     HNINNTIKLEILDTVRLLQLNNQRLSNGNYISNGIEFDKWHKPVNYYFCKFDPVTYTY
                     ITGDYEVIPAAEICHYFIADQQGQERGLPDMVATSKLMEDLKNFTEAALTAKRVSASS
                     MAFITNNNDTTSTDLLGADEREEVTPVYTEYFEAGFIGELAEGQDIKTVTPTNGVDGI
                     DQFTNELMNQISMGLNITKQALLSDTSNASFSASRLTEKLQQTTFRTRTNVLISKVLK
                     PIYIAWLKNEMLNNNKLNLSFSSFDDLICARYISQKPISLDPVKDIQAELLQLEAGIK
                     SKTQVIAELGGDPVKVLAEVQAEKDKENKNKEVIQDGNEKPEEGTNETPTGD"
     gene            3545..5389
                     /locus_tag="ACHXOX_003674"
     CDS             3545..5389
                     /locus_tag="ACHXOX_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR7007040.1"
                     /translation="MEMKNQKRELTKPLQAINTESRTIEVAFCSETPVEREINGQIYN
                     EILLCGSSNADLTRLNNNGAVLFNHNRDDLIGAVVDARVDADRVGRATLRISSTANDE
                     WEMIQEGVLTHISIGYNINDYQIDGNNIYVTNYEIYEISLVTVPADPTVGVGRSMDSQ
                     LALKSLNKEGETIQEGQMDMDNPENEKESIVDSTEEVNETIEEALVEPSVEYAEEIKQ
                     ELSDEELLEIISTRPDLLNKVHGEEPESINSTEDTERVRELEALGKVLNIDVSEAIET
                     GISISDFKRQLNDIKQNPLHDKEIKEMDNKNLLKDMVRAIKTGDKSALEAYERGINGF
                     VRAAVPSTNTTTAAGVVADDLQDQYIPELLKISALGELNTTVYSGLAGRGTLSIPKAA
                     GVSPVFKFYGEAEAQDDSIASFTKVTLSPKAFGGAIPLSKQAILTAPNIESFVQSELL
                     RYAAQGLEQNVMDKVVAAAPVFNVETAGSITLEDVQGAVAKLAQANVDMRGVKAVMNA
                     KTLSTLRQIAVLDNTAAKAMVEGYRSTEMWLADEVRVVISEFVADGTILMGDFSNVIL
                     ANWEGQEVDYDDTSYRSSNTIVYRVWDYSDIALAHSEAFVNIVIGKAE"
     gene            5403..5726
                     /locus_tag="ACHXOX_003675"
     CDS             5403..5726
                     /locus_tag="ACHXOX_003675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007041.1"
                     /translation="MRVFKDTQCDAFLNAFGQSIQTCTGSTFTGIVEVLPVSIEAAGG
                     FIESVETYLTIRKDDLITANVAIGTVLIIDGVNQTIYNIEDDLSGMVNCYFRTSAGAS
                     FAEDY"
     gene            5728..6120
                     /locus_tag="ACHXOX_003676"
     CDS             5728..6120
                     /locus_tag="ACHXOX_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007042.1"
                     /translation="MMLVQKVRNTMKQLINATKNLTVSRDVDVFEQIAFDYSLTSLSF
                     SNERRAGNFAIQYLISPKPESGNTAPSITYDQIISTFDKEKTSAFSDAGLIMLSCSYE
                     QSDIVTDPITGSVSLSFTINIQVTEKTR"
     gene            6138..8789
                     /locus_tag="ACHXOX_003677"
     CDS             6138..8789
                     /locus_tag="ACHXOX_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR7007043.1"
                     /translation="MSDIFSGRELSVQYNADIGNRTPQGAGNVVIKQINTFPTLTINS
                     EANNFETYDSDYRTVLLSDKSVAPFDIVVNYLPDDPTHQFLDNAAKSQQLFQIIIQYQ
                     MNKDENTITYAMVNGYITSYKLDGDKGSVVTKAYSFAPHDVIARAMTINALSPIYQGD
                     YGLGSNTTDVPQYSPSVPTGNSFIKVPSSQAGNPAGADLMGVGLVDGTTVSSLAMTKT
                     GTLSIYAKNATTAWTRIYTATQMDGRYVPLTRTINGHALTANLVLNSEDTGSVPDTRT
                     VNGKPLSEDVVLTSTDTGSVPDTRTVNGHVLSEDIVLSSEDTGSVPDTRTVNGHVLSE
                     NVVLSSEDTGSVPDTRMVNGHVLSEDVVLTSTDVGLGKVTDDAQLTIAGNLSDLGDVE
                     EARINLAVYSMDEVDAEVTALADSITSLDSASMKKASNLSDLSSLSTARTNLQVNRLT
                     QGATYTDLMSNDLTNRIRIQDNGAWGASTGTGWIALGIGQGGTGATDALSARLNFQVD
                     RFNQGSNLTTIKSADSSAALVVYDGGQWGSATYIAGAFTGWKALAIPQGGTGVKTQAE
                     LWPAIRPTGATSLAGDPVSDSDATTKRWVENLLSSRTSVSPQWDVIASSPSSTAIGSY
                     VTGGRIRSICNIGTSTEISGGFRVGKTMGQTGQADLTLFISENGAEDKLWGLKGPTGT
                     ILTDVQSGWMTNTAVSGPTDANTITFNGMYAGPGDSGINYSEKYSPLLHMQRYPGANR
                     SQLQIDGGGTLRVRGGNGTTWFGWLVMQAQGTSGREFKRDITPANPVEALDRIMSQDL
                     VTFIYKDDEQERVRFGVIAEDAELTAPQYIKHDEETVEIEGEIVTRDHPRVDLNPIVM
                     DLMGAIQAQQEEINQLKDLVQQLLNNK"
BASE COUNT         2826 a         1636 c         1910 g         2449 t
ORIGIN      
        1 ttatttaagt gatactggtt gtctggagat tcagggggcc agtctagaat gataagaacg
       61 ccgccaataa ctctaaacaa acacaaatgg tgaacggtac ttttatctat tggttgaacc
      121 tcaacacgcc ggggaacctt cgcggtatca caacacgctg tgttctgtta gatgaggtca
      181 gtagctgcga aatcactgat gaagggaacc caatcaaact ggcagaagca cgtaccagta
      241 ccttcggtag tgactcactg gtggttgtat cttccacgcc attgtataaa gacgatttaa
      301 tcaatgctga gtacaacctc agtgataaac gtaggtactt tgttactcac acatgcggtc
      361 atgaatatac ttttgaatgg gaacaagtag cgtttgaatt taagcaatta gaaaatggta
      421 gatccattcc tgatagtact actactcgtt tgttatgccc tcattgcaat gagggaatag
      481 atgaacatac acggcatcag atggttgata atggtcgatg gattgcaact aatccagatg
      541 gggaaccggg tgtagtaggg tatcaaatca gtagaatgta ttcacctctg aatactatta
      601 ctgaaatggt ttcaaagttt gctgatgctc tatataactt caatcttcaa accttttaca
      661 ataacgaatt gggcttgcca ttcgaagatg agtatcaaaa agaattagat attctccaat
      721 tagaatccct acgagaagat gaatttaacc tttataaaat tcctgaaagt accttagcgt
      781 tgtgtatttc agttgatcag cagggtgata ggctggaaag tctgctttta ggttttgatg
      841 aaaagaacat ttatgtactt gggcatgagt ttttctactc ccacgattgt acgaagattg
      901 aagccccagc ttggaaggat ttagatcagt tttgccgtca ggatttcagt actgtatcag
      961 gtcgcatagt acctacactg gctgtgttca ttgatgcagg gaacggtaac gctacggata
     1021 ctgtgaaaaa attcactaca cgttggagta agtatcatcc gatcaagggt agcagctcca
     1081 ctactggtga actattcaag accagtactc aggcggggta caaactacag atcctaaacg
     1141 tacatgaaca gaagaacacg atacgccgcc tcctgaacct gatgctatcc agtgaggcag
     1201 ataacgcccc ggttaagctc cgcttcagta gtaccttacc atcagatttc tttgagcaac
     1261 tttcagcaga ggagttaaaa cccgctggtg gtaagttggt atggcgatta aagaaaggtc
     1321 aaagaagaaa tgaggcttta gacctaatct gttatggaat gatcgccata gcatatgccc
     1381 aatcaaaatt aggtactcaa ccattcagga agttacgcga ttataaagca caggaaacca
     1441 ctaaatacga gataaataaa gtagaagaac caaaaccaga aacagtacaa aaaccaaaac
     1501 ggaatagacg tactggaatg ggatcaaact ggttcggtaa aagataagga tatcaaaaat
     1561 gacacttcta ccagaaaaga tctacatggt atcgaatccc atggatctaa cggtattagt
     1621 acccgctgct actattcttg ttattagttt tacatcatct ggaaaaagta cctcattaga
     1681 taatttaaat ggtagtactt caaaagaatt tacggttgta ttagatatga gtacagcaca
     1741 ggaattacta ttctgtactc aaatatctaa tggtcaaacc agtactttta cttctgaagt
     1801 cattaatcca ttactttata cttctgagta ctcccaatta aaacagatga tcagtgaaat
     1861 tgatcaggtt atagctaata aaattgcagg cggtgctaat tactcaataa caattaataa
     1921 taagactctt gtaagtgaat cattatcatc gcttgaatca atgcgggaac gctatgtaaa
     1981 acgtgctaat gctctatttg tgaaaatgaa tggcggtact ttctctaatg gcggtaaacc
     2041 aatcaagagt attacggttt tcaggcctaa gccagggagt acacgctaat gttctggaat
     2101 aagaaaaata aaaattcaga aactaaaccc aagaagatta aacaggaacg ttccttaaaa
     2161 caaagtacct taaaacgaga tctacaggca gtacgcaaca catcagtaat gaacttcgga
     2221 ttcaattcaa actcaggcag caatatcaac ttccttctga tgaaagcact acctaccttc
     2281 agggctttct cacgtgatgc agtacttaag aatcctattg gtcgtaagta catgaaccta
     2341 tcggtagatg gcgttgtagg ctctgatggc ctgtacgtta aaccttcagt agagattgac
     2401 ggtactgaag aagaaatcaa cggaatcaat ctaaccttgg aaaagctctt tgaccgttgg
     2461 gcatatgatg ctgatcgttt tagtgttgat ggctcattaa gttttgacct gtttcagcag
     2521 aacgcagaga aggtaagggt ccaggatggt gaatgcttta ttcgtataca caatattaat
     2581 aataccatca agttagaaat tctcgatacg gttcgtttgc ttcagttaaa taatcaacgt
     2641 ttgagtaatg gtaattatat tagtaatggt attgaatttg ataaatggca taagccagtt
     2701 aattactatt tctgtaaatt cgatcctgtt acctatactt atatcactgg cgattatgaa
     2761 gtaattcccg ctgcggaaat ctgtcactac tttattgctg atcaacaagg ccaggaacgc
     2821 ggattgccgg acatggttgc aacttccaaa ttaatggaag atctcaagaa cttcactgag
     2881 gctgcgttaa ctgcgaaacg tgtatctgca tcgtcaatgg cttttattac caataataac
     2941 gacactacca gtactgatct gttaggggct gatgaacgag aagaagttac accagtatat
     3001 acagagtatt ttgaagctgg ctttattggt gagttagccg aagggcagga tattaaaact
     3061 gttacaccta ctaatggtgt tgatgggatt gatcaattta ccaatgaatt gatgaatcag
     3121 atctcgatgg gtctgaacat aacaaagcaa gccttattat ctgatacatc caatgcttca
     3181 tttagtgcaa gccgacttac tgagaaatta caacaaacta cattccgtac ccgtactaat
     3241 gtactaatca gtaaagtact taaaccaatt tatatcgcct ggttaaagaa cgaaatgcta
     3301 aataacaata agttaaatct aagtttctcg agctttgatg atcttatttg tgctcggtat
     3361 atttctcaaa agccaattag tttagatcct gtaaaggata tacaggctga actactccag
     3421 ttagaagcag gtattaaatc taaaacacag gttattgctg agttaggcgg tgatccagtg
     3481 aaggtattag cagaggtaca ggcagaaaag gataaagaaa ataaaaacaa ggaagtaatc
     3541 caggatggaa atgaaaaacc agaagaggga actaacgaaa cccctacagg cgattaatac
     3601 agaatcgcgt accatcgaag tagcgttttg ttcagaaacc cctgtagaac gtgaaatcaa
     3661 cggtcaaatc tataacgaaa ttcttctatg tggttccagt aacgcagacc taacccgcct
     3721 taataacaat ggggcagtac tctttaacca taaccgtgat gatcttattg gtgctgtagt
     3781 tgatgcacga gtggatgctg atcgcgttgg tagagctaca ttacgaatta gcagtaccgc
     3841 caacgatgaa tgggaaatga ttcaggaagg cgtactaacc catattagca ttggctataa
     3901 cattaatgat tatcaaattg atggtaataa tatctatgtt accaactacg aaatttatga
     3961 aatctcatta gtaacagtac ctgccgatcc taccgttggg gtaggtcgtt caatggactc
     4021 acaacttgca ttgaaatcac taaataaaga aggtgaaaca attcaagagg gtcaaatgga
     4081 tatggataat ccagaaaacg aaaaggaatc aattgttgat agtactgaag aagtaaatga
     4141 aacaattgaa gaggcattag tagagccatc ggtcgaatat gcagaagaaa tcaaacaaga
     4201 attaagtgat gaagaacttt tagaaattat ctctactcga cctgatttgc tcaataaagt
     4261 acatggtgaa gaaccagaaa gtataaatag tacagaagat actgaacgtg ttcgtgaatt
     4321 agaagcactc ggtaaagtac ttaatattga tgtatctgaa gcaattgaaa caggaatttc
     4381 aatttcagat ttcaaacgtc aattgaatga cattaaacaa aatcctcttc atgataagga
     4441 aatcaaagaa atggataata aaaatctatt aaaagatatg gtacgtgcca ttaaaactgg
     4501 cgataaatct gctcttgaag catatgaacg tggtattaat ggttttgtac gtgcggctgt
     4561 accaagtact aatactacta ccgctgctgg cgttgtagct gatgacctac aagatcaata
     4621 cattccagaa ctactaaaaa tttctgcact tggtgaacta aacaccactg tttattctgg
     4681 ccttgcaggt cgcggtactc taagtattcc aaaagcggct ggtgtatctc cggtatttaa
     4741 attctatggt gaagcagaag cacaggatga ttctattgca agctttacta aagtaaccct
     4801 aagccctaaa gcttttggtg gtgctattcc actatccaaa caagcaattc taacggctcc
     4861 aaatattgaa tcatttgtac aaagtgaact actacggtat gccgctcagg gtcttgagca
     4921 aaatgtaatg gataaagtag ttgccgctgc tccggtgttc aatgttgaaa ctgctggttc
     4981 tattactctt gaagatgttc agggtgcagt agcgaagctg gcacaggcta acgttgatat
     5041 gcgtggcgtt aaagctgtta tgaacgctaa aaccctaagt accctacgtc agattgcagt
     5101 acttgataac actgctgcta aagcaatggt tgaaggttat cgcagtactg aaatgtggct
     5161 tgctgatgaa gtacgtgtag tgatttctga attcgtagca gatggtacta ttctaatggg
     5221 cgatttctcc aatgtaattc tcgctaattg ggaagggcag gaagtagatt atgacgatac
     5281 atcctatcgt tcaagtaaca ctatcgtata tcgcgtatgg gattactcag atatcgcact
     5341 tgcacactct gaagctttcg taaatatcgt tattggtaaa gcagaataat aaggattaga
     5401 taatgagagt atttaaagat acgcagtgtg atgcgtttct aaatgctttc ggtcagtcta
     5461 tccaaacttg tacgggaagt acttttacag gaatagtaga agtacttccc gtttctattg
     5521 aggcggcagg tggatttatc gaaagcgtgg aaacatacct aacaatcaga aaggacgatc
     5581 taataaccgc aaacgtggca attggtacag tactaatcat tgatggtgta aaccagacaa
     5641 tctataacat tgaagatgat ctatcaggta tggttaattg ctatttccgt acttctgctg
     5701 gtgcttcatt tgcagaggac tactaatatg atgttagtac aaaaagtacg gaatacgatg
     5761 aagcagctta tcaatgctac taagaatctg acagtatcac gtgatgttga tgtatttgaa
     5821 caaattgctt ttgattactc attaacgagt ttaagtttca gcaatgaacg ccgggcaggt
     5881 aactttgcaa ttcagtatct gatttcccct aaaccagaat caggtaatac agccccatcc
     5941 atcacttatg atcagattat tagtactttt gataaagaga aaacttcagc attcagtgat
     6001 gcaggtttaa taatgctttc gtgttcttat gaacaatccg atattgtcac agatcctata
     6061 actggctcgg tttccctatc ctttacgata aatattcaag ttacggaaaa gacaagataa
     6121 ctaaaaggat ataaaggatg agtgatattt tctcaggaag agaattatca gtacagtata
     6181 atgctgatat tggtaacaga acaccacagg gagcaggcaa tgtagtaatt aaacagatta
     6241 atacttttcc aaccttaaca ataaattcag aagcaaataa ttttgaaacg tatgatagtg
     6301 attacaggac agtactacta tctgataaat cagtagcacc atttgatatt gtagttaact
     6361 acctacctga tgatcctaca catcaattct tggataatgc cgctaagtct cagcagcttt
     6421 tccagatcat cattcagtac cagatgaata aggatgaaaa taccattact tatgcgatgg
     6481 tgaatggtta tatcacctct tataagttgg acggtgataa aggttcagta gtaaccaagg
     6541 catacagttt tgccccacat gatgtgatcg cacgtgcaat gaccattaac gcactatcgc
     6601 caatctatca gggggactac ggtttaggtt cgaacactac ggatgtacct cagtactcac
     6661 catcagtacc tacgggtaac agcttcatca aagtaccatc atctcaagca ggcaatcctg
     6721 ccggggcgga tctgatggga gtagggcttg tagatggcac tactgtaagt tctcttgcga
     6781 tgacaaagac gggtactcta agtatctatg caaagaacgc tacaaccgcc tggacacgca
     6841 tatataccgc tacacagatg gacgggcgat acgtacccct tacacgtacc atcaacggcc
     6901 atgcactcac ggccaattta gtactgaaca gtgaagatac aggttcagta cctgatacac
     6961 gtactgtgaa tggtaagcct ctaagtgagg atgtagtact taccagtact gataccggct
     7021 ctgtaccaga tacacggaca gttaatggtc atgtactcag tgaagatata gtactgagta
     7081 gtgaagatac aggttcagta cctgacacgc gtacagtgaa cggtcatgta cttagtgaaa
     7141 atgtagtact gagtagtgaa gatacaggtt cagtacctga tacacgtatg gtcaacggtc
     7201 atgtactcag tgaggatgta gtacttacca gtactgatgt tggcttaggg aaggttacag
     7261 atgatgccca gcttacgata gcaggtaatc taagtgatct gggggacgtg gaagaagcac
     7321 gtattaacct tgctgtttac tcaatggatg aagtagatgc agaggtaacg gctttagctg
     7381 acagtattac ttcccttgat tccgcttcca tgaagaaagc gagtaacctt tctgatctat
     7441 ccagtctctc tacagcacgt actaacctac aggtgaaccg tttgactcag ggtgctacgt
     7501 acaccgatct gatgagtaat gacctgacta accgtatccg aattcaggat aacggggcat
     7561 ggggtgcaag tactggtact ggatggattg ctttaggtat aggtcagggg gggacagggg
     7621 ctacagatgc tttgagtgct cgcctaaact ttcaggtgga tcgctttaat caaggtagta
     7681 atttaacaac tattaaatcc gctgattctt ccgctgcatt agtggtttat gatggagggc
     7741 aatggggttc agctacatat attgcgggtg ctttcactgg ctggaaagca ttagcaattc
     7801 cgcagggcgg tactggtgtt aagactcagg ctgagttatg gcctgcgatt cgtcctacgg
     7861 gtgcaacttc tttagcagga gatcctgtct ctgattctga cgcgactacc aaacgctggg
     7921 ttgaaaactt gctgagtagt cgtacttcag ttagtcctca atgggatgtt attgcttctt
     7981 ccccttcctc tacggcaatt ggtagttatg taactggagg gcgtattcgt tcgatatgca
     8041 acattggtac atcaactgaa atatctggtg gttttcgggt tggtaaaacg atgggacaga
     8101 ctggacaggc tgatctcacc ctttttattt ctgaaaatgg tgcagaggat aaactatggg
     8161 ggctaaaagg gccgactgga acgatcctaa ctgatgttca atcaggatgg atgactaata
     8221 ccgctgtttc gggaccgaca gatgccaata ccatcacctt taacggtatg tatgcagggc
     8281 caggtgactc aggtattaac tatagtgaaa aatattcccc tttattgcac atgcaaagat
     8341 acccaggggc aaatcgctct cagcttcaga tagacggtgg tggtacttta agagtacgag
     8401 gcgggaacgg tacaacatgg tttggatggt tagtaatgca agctcaaggt acatcaggtc
     8461 gtgagtttaa aagagacata actccagcaa atccagttga agcattagat cggataatgt
     8521 cacaagattt agtaacgttt atctacaagg atgatgaaca agaaagggtt aggtttggtg
     8581 ttatcgccga agatgctgag ttgactgctc cgcagtacat taagcatgat gaagaaacag
     8641 ttgaaataga aggggagatt gtcactcgtg atcatccacg tgttgattta aatccaatag
     8701 tgatggactt gatgggagct attcaagccc aacaagaaga aattaatcag ctaaaagatt
     8761 tagtacaaca gctattaaat aataaataaa tataaacggg gtgaacggat tcactccaat
     8821 a
//
LOCUS       DAZRVY010000153         8780 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZRVY010000153 DAZRVY010000000
VERSION     DAZRVY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.153"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHXOX_003678"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHXOX_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR7007044.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXOX_003679"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXOX_003679"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR7007045.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHXOX_003680"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHXOX_003680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR7007046.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXOX_003681"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXOX_003681"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR7007047.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXOX_003682"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXOX_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR7007048.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHXOX_003683"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHXOX_003683"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR7007049.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHXOX_003684"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHXOX_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR7007050.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         2096 a         2197 c         2379 g         2108 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac
//
LOCUS       DAZRVY010000154         8726 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZRVY010000154 DAZRVY010000000
VERSION     DAZRVY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.154"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHXOX_003685"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHXOX_003685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR7007051.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHXOX_003686"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHXOX_003686"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR7007052.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHXOX_003687"
     CDS             complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHXOX_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR7007053.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHXOX_003688"
     CDS             complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHXOX_003688"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR7007054.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2136 a         2025 c         2232 g         2333 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggt gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaacgt cactgaagag
     4921 agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc agatttcttc
     4981 tggctactga tggttttaat actgcggttc agctttttag cgatctcggt caccaggaag
     5041 ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt gtcaccgtaa
     5101 ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa tttcttccct
     5161 ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg caccttgttt cagcacgatc
     5221 ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt catagtcaga
     5281 acaatgatcg acaggcttgg gaaatggcgc ttgatgtact tgattaaggt aatgccatcg
     5341 ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc cagtttcggc
     5401 aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac ccactcaatt
     5461 tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc aataattacg
     5521 ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     5581 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     5641 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     5701 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     5761 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     5821 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     5881 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     5941 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     6001 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     6061 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     6121 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     6181 cgggccagcg gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     6241 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     6301 cgggtccgcc tttccataac gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     6361 atccatttca tgaatactta cgccttctcc ggtgtccaga atgcggaatg tcaggcggtc
     6421 ttcagaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     6481 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     6541 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     6601 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     6661 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     6721 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     6781 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     6841 gccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     6901 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     6961 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     7021 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     7081 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     7141 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     7201 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     7261 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     7321 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     7381 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt ctttctcatt
     7441 atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     7501 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     7561 ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     7621 gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacattt ctgcacgacg
     7681 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     7741 cagggttgag atcagtacca gactgttatc ctgaccattc aggtaataca tcgaccacgg
     7801 tacattttct gcaccccaca atgtatccag ataagtggac atccgctgag tcatctcaag
     7861 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgtg gtttctccag
     7921 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     7981 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     8041 ttggattgcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     8101 ggcgctttgt accattaccc ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     8161 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     8221 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     8281 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     8341 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     8401 gcccaacgaa attaattgca aataaacgaa gattgctgga aattatgctg atgttaatta
     8461 tttgtgaaat agttgacaag agttatagtt tttctgtggt agcacagaat aatgaaagtg
     8521 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg ggtaaacaga
     8581 cattcagaaa tgaatgacgg taataaataa agttaatgat gatagcggga gatattctag
     8641 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
     8701 gattttaacc ttgaattatt attgct
//
LOCUS       DAZRVY010000155         8696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZRVY010000155 DAZRVY010000000
VERSION     DAZRVY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.155"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHXOX_003689"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHXOX_003689"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR7007055.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHXOX_003690"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHXOX_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR7007056.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHXOX_003691"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHXOX_003691"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR7007057.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHXOX_003692"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHXOX_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR7007058.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHXOX_003693"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHXOX_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR7007059.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHXOX_003694"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHXOX_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR7007060.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHXOX_003695"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHXOX_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR7007061.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHXOX_003696"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHXOX_003696"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR7007062.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHXOX_003697"
     CDS             6614..7768
                     /locus_tag="ACHXOX_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR7007063.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHXOX_003698"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHXOX_003698"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR7007064.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZRVY010000156         8632 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZRVY010000156 DAZRVY010000000
VERSION     DAZRVY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.156"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHXOX_003699"
     CDS             complement(143..2686)
                     /gene="mdoH"
                     /locus_tag="ACHXOX_003699"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR7007065.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHXOX_003700"
     CDS             complement(2679..4214)
                     /gene="mdoG"
                     /locus_tag="ACHXOX_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR7007066.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHXOX_003701"
     CDS             4608..5765
                     /gene="mdoC"
                     /locus_tag="ACHXOX_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR7007067.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHXOX_003702"
     CDS             complement(5773..7254)
                     /gene="clsC"
                     /locus_tag="ACHXOX_003702"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR7007068.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHXOX_003703"
     CDS             complement(7196..7729)
                     /gene="ymdB"
                     /locus_tag="ACHXOX_003703"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR7007069.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHXOX_003704"
     CDS             complement(7824..8135)
                     /gene="ymdA"
                     /locus_tag="ACHXOX_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR7007070.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHXOX_003705"
     CDS             complement(8256..8588)
                     /gene="csgC"
                     /locus_tag="ACHXOX_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR7007071.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2246 c         2051 g         2180 t
ORIGIN      
        1 ataacgccgg tatttttatt ggctcgttgt ttttgccgga tgcgacgtga acgctttacc
       61 taacccacaa aagcttgcaa aatcaataaa ttgcatgaac gatgtaggcc tgataagcgt
      121 agcgcatcag gcaactacat ttttattgcg aagccgcatc cggtttacgc aatgccagtg
      181 gattgagctt tatcccttcg taataactca cccatgaaga atatctctca ggggaattcc
      241 agacgcggaa atgcagacgg gccatcgtca ccggatcgct tagcagcaca aggcgacgat
      301 cgcgattcag cttctctggc gtctcgttca gcgcctgttc aacgtggcgg tcacgggcga
      361 tttccagcac cttactggcg cggtgacgcg cggtcgccat tgcggttgcc agagcgttaa
      421 atgacgggtt aaacactgcg tgcataaagc catcgtcaag ggagcgttgg cgattcatct
      481 caaggaaccg atcggtatca accagcacct gcggcggtga atactcttcc gggatcagga
      541 acagtttcca gcgtttggtg cgcagaccaa cggtggcacg gctggaaatc accgaaacaa
      601 acggtgacag gatcaacgag aagacaatcg gtgccagcca gaacaggaaa cgcagatcca
      661 gccacgccat cccaacagcc cacactaacc ctaacagcag ctgtgagccg tggcgtttga
      721 acgcttcacc ccaggaagtg gagtcatcat cacgctgcgg tgaattccac accacttccc
      781 agccaaggaa cgcgctgacg acgaagaccg tatggaacag catgcgtacc ggagccagca
      841 gcacggaaaa cagcacttcc agcagcaacg ataatgtaac gcgccagaag ccgccgtatt
      901 ctttcgttcc tttgcaccag ataagcaaaa tgctcaataa cttcggcagg aacaacagca
      961 ccatggtcga agcaaaaagt gcaatcgcca gctcaggacg ccactgcggc cacaccggga
     1021 acaactgccg tggttgcagg aagtattgcg gttcggtcaa cgcatgtact acctgcaatg
     1081 cagtagagag cgcgaggaac ataaaccaca gcggagcgga gagataagac atcacgcccg
     1141 tcaggaacac cgcacggtga accgggtgca tacccttcac caggaacaga cggaagttca
     1201 tcaggttacc gtggcaccat cggcggtcac gttttagctc atcaagcaag ttaggcggca
     1261 attcttcata agaacccggg agatcgtaag caatccagac cccccaacct gcacggcgca
     1321 tcaacgccgc ttccacgaag tcatgtgaca ggattgaacc ggcaaaggaa ccttcgcccg
     1381 gcagcggagc cagtgcgcag tgctcgataa acggtttcac gcggataatc gcgttatgtc
     1441 cccagtagtg cgactcgcca agttgccaga agtgcaaacc ggctgtaaat agtggcccat
     1501 acacgcgggt cgcgaactgc tgacagcgcg catacagcgt atccataccg gacgctttcg
     1561 gcgacgactg aatgatcccg gcgttcgggt tggcttccat caggcgcacc agcccgcaca
     1621 aacaatcacc ggtcattacc gagtcagcat ccagcaccac catgtagctg tactggctgc
     1681 cccagcgacg gcagaagtca tcgatattac cgcttttacg cttcacgcgg cgacggcggc
     1741 ggcgatagaa aatctgacct tcgccaccga cttcagcgat aagctccatc caggcttttt
     1801 gctctgcgac gcagatatcc gggttataac tgtcactaag aatgtagaca tcaaagtgtt
     1861 tggcattccc ggtggctttt actgattccc acgttgcacg caggccagca aaaacacggt
     1921 tcacgtcttc gttacagata ggcatgatca acgccgtgcg atgctccggg tttaatggtt
     1981 catcgccaac tgttgacgca gatatactgt atttatcgcg accaataagc agttgcagga
     2041 agcccattaa cgccgtccag aatccggcgg acacccaaca gaacagtacc gcaaagagga
     2101 tcaggatacc ggtttgcagc atataaggca gaagctgcat aaaggaaacc cacaaatcct
     2161 gaccaaccat atccatagga ttaatcagcg cccaaccctg ataaggaaga atggtcttca
     2221 tataccaggt cgcgacgaca gtttgcgcga gcgtcaggat caacagaatg taacggcgga
     2281 tggtaccgac ggtacgccac ttttgctcgc tctcctgctc ttctttggtc aaacgagcca
     2341 gatagcgcgg cgtgacatcg cgtccacgca ggcgatccca gaaacggcct accgggttgg
     2401 tacgccacgg gtcgggaaac atcgaggagc gttttgcttc tggcatcgcc ttcagctgat
     2461 cgcgcccttc gtcgtcttta attaactgtc catcagcaag tgaatctggc caggcttgtt
     2521 ccagacgcgc ctttaccgag ccttgcgggg aatcatcctc ccgcgcccag gtgcggtgtt
     2581 cggcatccag cgcctgatga acggcgcgga tatcagtctt cggcaatgcc gctttctcgc
     2641 ttgcggcgat gggcattgcg tcaatgtact cagttgtctt attcattggc aggtaactgg
     2701 tagctccagg tttcactcaa cgtctgatcg gcattcacca gcgcagcacg catttcagtg
     2761 gttttcttgg catctttcac tttcacacgc atcaccagac gccagccttt ggtaaccggg
     2821 ttatagcgca ccgtgctttc aactatctca ccattatcac caatgctggt ttgcgctgtg
     2881 accggggtat cctctggcag ttttttcatc tctgcgccgg taaaatcgac cacaaaggcg
     2941 atagtaccgt caggctggcg aatcaggttc gactgcttca catcccccgt tgaacgacgc
     3001 gtttgttgca cccatgcgtt atctggcgca tgcagtttgt cttcatcacg gctgaaggtg
     3061 atggtgtatt taaagttcat ctctttaccc ggctccggca gctgatccgg cgtccagtaa
     3121 gcgacgatgt tatcgttggt ttcatcgttg gttggaattt ccaccagctc aacgctgcct
     3181 ttaccccact cccctttcgg agtcacccat gcgcttggac gaagatcgta acgatcatcg
     3241 agatcttcaa agcgggagaa atcacgaccg cgctgcaaca gaccgaagcc ttgcgggttt
     3301 tccatcgaga agctgctaac cgctaaatgt ttcgggttat tcaacggacg ccagatccat
     3361 tcgccattac cggcatggat agagagaccg ttagagtcgt gcaactccgg acgatagtta
     3421 tttgcaggcg acggttggtt cggcccaaac aggaacatac tggttaacgg tgcaaccccc
     3481 agtttgccga ctttatcgcg cagatagatt ttcgactgca catccacaac cgtgtcacgc
     3541 cctggcataa ccacgaattt gtaagcacct gtcgcgcgcg gcgagtcaag caatgcataa
     3601 atggttaaac gtttatcagt cggttttgga cgctcgatcc agaactcttt gaagcgtgga
     3661 aattcttccc ccgatggcaa ggcggtatca attgccaggc cgcgggcaga aaggccataa
     3721 acctgacctg caccaatcac gcggaaatag ctggccccga gcatgctgac gatttcatcg
     3781 tttttatctt tgctgttgat cgggtaaagc actttaaaac cggcaaaacc aaggtctttt
     3841 accgtgtctt tgtcatgctg aacatcgccg aaagtgaaat aatccgggct gtatttgatt
     3901 cgtttgactg cggtggcagt cacttcattt attttgaccg gggtatcgaa gtacataccc
     3961 tgatggtaga actcgagttt gaatggggtc ttcagattgt tccagtacgc tttgtcatga
     4021 ttaaactgga tctgctgata gtccgcgtat ttcatatcgc ggaaaacgga gggcaagttg
     4081 cttttgggcg cctcatagcc tttcccggct aaggattgag cttgctttgc gacatcatca
     4141 atactgaaag cccagcttga agatgtatac agggttaaca ttactgcagc actcaaccaa
     4201 cgcattttca tcatttgtag tttatgtttc ataattagta agcacttccc cctttgtgtg
     4261 cttatatcga tccgatctat tttaatggat aaccagccat tccgacaaca gaatcccggt
     4321 atcgttcagc acgctggctc atggattaag cacctgattg caaccgtttt cactttccgt
     4381 gcttctccta aagacagaag catcgacttc gtgtagggtt tgccagcaat tttatttatc
     4441 aaccgtctta cagacgcttc cattctggtt agtggtagcg atgatgctat gcataaaaac
     4501 cttgaagcct gcgcctggcg cggtcaggtc ggtgtcgcta tggcaacaag cctaaaggca
     4561 taacccgaca gggataagac gaaaaatccg agattagtta accatatatg aatccagtac
     4621 ccgcgcaacg tgaatatttc ctcgactcca tccgcgcctg gctgatgttg ttagggatcc
     4681 cttttcatat ttctttaatc tattcgagcc atacatggca tgtgaatagc gccgaaccgt
     4741 cattgtggct gacccttttt aatgacttca tccactcgtt ccgcatgcag gtatttttcg
     4801 ttatatccgg ctacttttcc tacatgcttt ttttacgcta tcccttgaaa aaatggtgga
     4861 aagtacgtgt cgaacgtgta ggtatcccga tgttaacagc catcccctta ctgacattgc
     4921 cgcaatttat tatgctgcaa tacgtcaaag ggaaagcgga aagttggcct gggctgtcat
     4981 tgtatgacaa atataatacg ttggcctggg aattaatatc acacctgtgg tttttactgg
     5041 tgttagtagt catgacgacg ctgtgcgtat ggatatttaa acgcatcaga aataatttag
     5101 aaaattctga taaaacgaat aaaaaattct cgatggtaaa actatcggtg atttttttat
     5161 gcctcggcat cggctatgcg gtaataagaa gaacgatttt tattgtgtat ccgcccattc
     5221 tgagtaatgg catgttcaat tttattgtca tgcaaacgct attttatttg ccgttcttta
     5281 tcctcggtgc actggctttc attttccctc atcttaaagc cttgtttacc acgccgtctc
     5341 gtggctgtac ccttgcagca gcattggcat ttgtcgctta tttactcaac cagcgctatg
     5401 gcagtggcga tgcctggatg tacgaaaccg agtcggtgat caccatggtc ctcggtctgt
     5461 ggatggtgaa tgtggtcttc tcctttggcc accgtttgct taacttccag tcagcgcggg
     5521 tgacttactt tgttaacgca tcgctattta tctatctggt tcaccacccg ttaacgctgt
     5581 ttttcggcgc atacattaca ccgcacatca cctccaactg gcttggtttt ctctgtggcc
     5641 tgatattcgt agtagggatt gcgataattc tgtatgaaat tcatttacgc atcccgttac
     5701 tgaagttttt gttttctggt aaaccggttg ttaagcgtga gaacgataaa gcaccagccc
     5761 gttaagccac atttacaata accattccac gggcaatatc gacgccagtc tgaccataac
     5821 ccgcttccag aaactggtgg cgggttcttt tttgagaata atctctttct ttgcatgacg
     5881 atcaacccag ttgatccgtc cccacctgtc cagacggagc tgccaggccg catcatactg
     5941 gctctgaata aagcgtttat caattaactg tgccagcgtt tcgctctcta tcacgaagcc
     6001 catttcagta ttgagcaatg ttgaacgcgg atcgaaattg aaagagccga taaacaccgt
     6061 tttaccatcg atgctaaaag ttttagcatg caggctggct ccggaattac cggttatgcc
     6121 gcgatcgtgt aacgtactac tttgttcacg cgtcggcttg agttcatata attccacgcc
     6181 atagcggagc aattttttgc gccagcgcgc gtatccggca tggacgacag caacatcgtt
     6241 agcggcaaga gaattggtta ggatcgcaat ctttacccct tttctcacca tccgtaagag
     6301 ttgcgccaca cctgcgcgtg tcggtacaaa ataggaagag ataatatcga tgcgttcact
     6361 gggtgagccc atgatatcgg acaggcgctg cggtaacagt gaatgccgtt ttgccttgcc
     6421 ctcccctttc gccggatcat cacttaataa acgtgttttc gcccagataa gcggcaacgt
     6481 tccatcaacc agatgattta taaacggact ggattccatt tggcgtaagt aacgatgcgt
     6541 catggcatcg ttatgccagg aggcgggtaa ctcgatgcga tccgccattt caccttccgg
     6601 gacatccagc acctgctgta agggtgaaac cgatttgcaa taccagtagc gggcgaaatc
     6661 atcggcaacg tcctctacca cgggtcctat tgccataaca tctaaatccg aaaaaagtgg
     6721 ctcttcccct gccccaaaat aggcatcacc aatatttcgt cctcccacca gggtaaccac
     6781 gccatcgaca gtgaaacttt tattgtgcat acggcgattg agacgggaaa agtcggtgat
     6841 ataaccaagc ggacgcagca agcgaaacga gaaaggatta aaaagccgga cttcaatgcg
     6901 gggatggctg tcaagcaagc gtaaaatatc gtcaagtccg ggcgtattgt tgtcgtccag
     6961 caacaaacgg acgcgaacgc cacgctttgc tgcggctaac agggcggaaa acagtaaccg
     7021 acccgacata tcgtcctgcc agatgtaata ctgaacatcc agcgtatgct ctgccatttc
     7081 ggccagacga taccgggcgg caaacgcatc cagacttttc tccagaggga aaagaccaca
     7141 ctgaccggga tgttgcgaac acagtggcag caccgcgctc gccagccggg gcaaatcatt
     7201 catctccttg ttgggtaagg agtctttcgt agaggtgggc gttttcttca tcataacaga
     7261 caaagtatac ctgttcgggt aaagcgtgac gggtaataaa ttctgaaacg gttttaactg
     7321 cgatttcagc cgctgccgca cgagggtaac cataaacccc agtactgatt gcaggaaaag
     7381 ccactgacgt atagctgttt gccgccacca gtcgcaggct attcagatag gcatcctgca
     7441 aaagctggtc ttcgttttgt tcaccaccac gccagactgg cccgacggtg tgtactacgg
     7501 ctttagcggg aagatcgcct gcaagcgtaa taacggcatg ccccgtaggg caatcgccct
     7561 gctgttgcct gacttttaaa caggcatcca gcagggccgg acccgctgcg cgatgaatgg
     7621 ccccatcgac gccgccgcct cccattaatg acggattagc cgcattcaca atcacatcaa
     7681 cggccagttt ggtaatatca ccctgcacaa catgaatacg cgttttcata acgcctcctt
     7741 acaccttctt gtttcttaag tgtacagcag gctttttggt taaatcgcat ttttctgctc
     7801 agcaacgtgc gttgttcaga aacttagtta tattcgatgt tcattacagc cagctttttc
     7861 tgtttatcaa gatactgcac ttttactgag gctatttgcg cgtttttaac attaccgctc
     7921 attaacgctt tgctggaata ggttttgccc ggaacagaac cattctgtgg gcagttcatt
     7981 tctacgggtg atgactgggt gctgacgtca catgccggtt ccacaatttg gccataaaaa
     8041 tggatcacac ccccttgcgc ggtgcttcct gcgatggcaa taaaaggaaa aaacaaactg
     8101 ccgagcataa gagagtttaa aaaaggtctg aacatttatt ctatttcctc aatgattagt
     8161 catccttgag ggttgtgtta tccatacttt caggttggct gattcgcagc agaccattct
     8221 ctccagattc atcttatgct cgatatttca acaaattaag acttttctga agagggcggc
     8281 cattgttgtg ataaatgaag tgcctgtcca tcagaaacag taacaactat tttcacccga
     8341 tcttccgggg aaatatttaa actcaacttc gtcaaagcaa taggttgatt agcaggcaat
     8401 gagagggtct tttcttgctt cgtctgactt tgccctgaac tgccttcgcg cagggacaat
     8461 atttgtactc tgcacagaca agattgagta agagtgactt caggaataat ggtatacaca
     8521 tccccttgct gggtcgtatt aaaggttatc tggctggaaa gtgccgcaag gagtaataac
     8581 gcattcatat tcttctcccg aaaaaaacag ggcttgcgcc ctgtttctgt aa
//
LOCUS       DAZRVY010000157         8608 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZRVY010000157 DAZRVY010000000
VERSION     DAZRVY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.157"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(241..447)
                     /locus_tag="ACHXOX_003706"
     CDS             complement(241..447)
                     /locus_tag="ACHXOX_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR7007072.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            760..849
                     /gene="kdpF"
                     /locus_tag="ACHXOX_003707"
     CDS             760..849
                     /gene="kdpF"
                     /locus_tag="ACHXOX_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR7007073.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            849..2522
                     /gene="kdpA"
                     /locus_tag="ACHXOX_003708"
     CDS             849..2522
                     /gene="kdpA"
                     /locus_tag="ACHXOX_003708"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR7007074.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHXOX_003709"
     CDS             2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHXOX_003709"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR7007075.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHXOX_003710"
     CDS             4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHXOX_003710"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR7007076.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHXOX_003711"
     CDS             5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHXOX_003711"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR7007077.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHXOX_003712"
     CDS             7848..8525
                     /gene="kdpE"
                     /locus_tag="ACHXOX_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR7007078.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1811 a         2225 c         2478 g         2094 t
ORIGIN      
        1 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
       61 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      121 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      181 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      241 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      301 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      361 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      421 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      481 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      541 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      601 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      661 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      721 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      781 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      841 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      901 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
      961 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1021 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1081 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1141 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1201 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1261 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1321 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1381 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1441 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1501 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1561 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1621 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1681 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1741 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1801 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1861 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1921 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     1981 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2041 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgattg
     2101 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2161 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2221 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2281 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2341 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2401 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2461 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2521 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2581 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2641 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2701 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2761 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2821 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2881 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     2941 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3001 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3061 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3121 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3181 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3241 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3301 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3361 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3421 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3481 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3541 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3601 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3661 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3721 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3781 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3841 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3901 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     3961 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4021 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4081 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4141 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4201 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4261 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4321 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4381 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4441 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4501 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4561 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4621 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4681 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4741 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4801 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4861 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4921 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     4981 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5041 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5101 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5161 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5221 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5281 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5341 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5401 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5461 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5521 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5581 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5641 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5701 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5761 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5821 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5881 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     5941 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6001 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6061 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6121 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6181 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6241 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6301 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6361 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6421 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6481 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6541 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6601 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6661 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6721 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6781 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6841 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6901 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     6961 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7021 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7081 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7141 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7201 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7261 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7321 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7381 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7441 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7501 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7561 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7621 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7681 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7741 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7801 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7861 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7921 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     7981 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8041 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8101 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8161 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8221 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8281 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8341 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8401 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8461 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8521 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8581 cgaatattat tatttggcag gtttaatt
//
LOCUS       DAZRVY010000158         8572 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZRVY010000158 DAZRVY010000000
VERSION     DAZRVY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.158"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            126..449
                     /gene="soxS"
                     /locus_tag="ACHXOX_003713"
     CDS             126..449
                     /gene="soxS"
                     /locus_tag="ACHXOX_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR7007079.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(452..2038)
                     /gene="pdeC"
                     /locus_tag="ACHXOX_003714"
     CDS             complement(452..2038)
                     /gene="pdeC"
                     /locus_tag="ACHXOX_003714"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR7007080.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2468..2748
                     /locus_tag="ACHXOX_003715"
                     /pseudo
     CDS             2468..2748
                     /locus_tag="ACHXOX_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2847..3383)
                     /gene="ssb1"
                     /locus_tag="ACHXOX_003716"
     CDS             complement(2847..3383)
                     /gene="ssb1"
                     /locus_tag="ACHXOX_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR7007081.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3637..6459
                     /gene="uvrA"
                     /locus_tag="ACHXOX_003717"
     CDS             3637..6459
                     /gene="uvrA"
                     /locus_tag="ACHXOX_003717"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR7007082.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6494..6850)
                     /locus_tag="ACHXOX_003718"
     CDS             complement(6494..6850)
                     /locus_tag="ACHXOX_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR7007083.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6854..7270)
                     /locus_tag="ACHXOX_003719"
     CDS             complement(6854..7270)
                     /locus_tag="ACHXOX_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR7007084.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7381..8094)
                     /gene="aphA"
                     /locus_tag="ACHXOX_003720"
     CDS             complement(7381..8094)
                     /gene="aphA"
                     /locus_tag="ACHXOX_003720"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR7007085.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2169 a         2140 c         2104 g         2159 t
ORIGIN      
        1 gggttagcag cgctttaatg cggggtaatt tcttttccat aaatcgcttt acctcaagtt
       61 aacttgagga attatactcc ccaacagatg aattaacgaa ctgaacactg aaaagaggca
      121 gatttatgtc ccatcagaaa attattcagg atcttatcgc atggattgac gagcatattg
      181 accagccgct taacattgat gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc
      241 aacgaatgtt ccgcacggtg acgcatcaga cgcttggcga ttacattcgc caacgtcgcc
      301 tgttactggc cgccgttgag ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg
      361 acctgggtta tgtctcgcag cagaccttct cccgcgtttt ccgtcggcag tttgatcgca
      421 ctcccagcga ttatcgccac cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg
      481 gtaattgctc catccattgc ataaacacct gcggcggcat cgccttcgca aaaaaccatc
      541 cctggcaata gcgcacgccg cgtttgcgca gccagttaac ctgctcctca gtttcgacgc
      601 cttcagcgat cgtttttaac cccaggctgt gcgccagctc gatgatgtgt tccgcaatca
      661 aatgactggt tttgtgggtg gtcagcgttt caacaaacga tttatcgatt ttcaaaatat
      721 cgacattcaa tgatttaagg ttatgcaagt tagagtagcc aataccaaaa tcatcaattg
      781 ccacttcgta accagcctgg cggaaagcca gaataatcgg cgtcattttt tcgacatcaa
      841 gaaatgcatg ctcagtcact tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg
      901 ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa
      961 tagaaacata gcgatcggca tgtgttgcca agtacgcgcc cagatcgcgg aagacattat
     1021 caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc ggaataaact
     1081 ctgccggatt cattatttgc ccctgctcac caagccaacg taacaacgct tcagcgccga
     1141 tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt tgatgttttt
     1201 cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata cgtagccaga
     1261 gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata agatgattat
     1321 aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg
     1381 tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa
     1441 tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca
     1501 ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca ctccagaaga
     1561 gagggttgat aaccgccaca taatttcccc gctgcatata ggtcatttta tagccagaga
     1621 aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta taatcggccg
     1681 gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa tggttatcac
     1741 gggcatagat caattcatta atataaagat agccacgaat aatattcaac attcgctttt
     1801 gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc actaaatctg
     1861 cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt cggagctgat
     1921 tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg atgggaaaga
     1981 gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga tgactcattg
     2041 ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaaccct gggtaccgac
     2101 gtctttagat aaatcttacg gtataaatag cacttttgcc tgatttgaaa ttgatacctt
     2161 tctaatcgtc acctggatga aaataattca ttgatattca aaattaattt cctacaggaa
     2221 tgcagaggcg gcgggaagat atgcccttaa tgccactgaa aaaccacata atctgtacag
     2281 caataaaagt cacggcctaa tatggctaac aaacaatcaa ttgtgatata gctcacaaaa
     2341 ttaatgaaac aaacagagtg tttcattttt gtgttctgca agccccgcga tagccagagt
     2401 gtaccacgcc tcccgtgaac aacgccgcgc tgtccagggc atcggctctt ttcagaggaa
     2461 ttgtttgatg gctaccctca ccactggcgt ggttcttctt cgctggcagc ttcttagtgc
     2521 cgtaatgatg tttctggcca gcacatcaac atccgttttc gtcggtctga ttatgtcggg
     2581 cttgcggtga tcagcagcgg tctgggcgtt gtttccgcct gttggttcgc aatggggttg
     2641 cttggcatca caatggcgga catcaccgct atctggcaca acatcgagtc ggtgatgata
     2701 gaagagatga atcagacacc gccacaatgg ccaatgattt tgacctgata cgtagaagcc
     2761 tccataacgg aggcttcttt tttatggctg gcagatgctt taatcatcca ccttaaaaca
     2821 atataaccta ttgttttaat gacaaatcag aacggaatgt catcatcaaa gtccatcggc
     2881 ggctcgttag acggcgctgc cggagcggac tgctgcgggc gagactgcgc gccgccgctg
     2941 aactgattgc caccctgcgg ctgctgaggc tgaccccaac cgccctgcgg ctgaccacca
     3001 ccgatattac cacctgccgg agcgccacca ccctgacgac cacccagcat ctgcatggtg
     3061 ccgccaacgt tcaccacgac ttctgtggtg tagcgatcct gaccggattg atcggtccat
     3121 ttacgggtac gcagctgacc ttcgatataa acctgagaac ctttacgcag atattcgctg
     3181 gccacttctg ccagtttgcc gaacagcaca acgcggtgcc attcagtctg ttctttcatc
     3241 tcgccggtcg ctttatcacg ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg
     3301 ccaccatttg gcatgtagcg tacttccggg tcctgaccca gattaccaac gagaataacc
     3361 ttgtttacgc ctctgctggc catgttcgtg tctcctgaaa aaaatcgttc tgaataagtg
     3421 taaacgcgcg attgtaccat taccaatagc gcttttacta tgttgtgacc tcggttccgg
     3481 gaaacaaacc tggccagaca ttgttacaca acactccgga taatgcattc caatactgta
     3541 tattcattca ggtcaatttg tgtcataatt aaccgtttgt gatcgccggt agcaccatgc
     3601 caccgggcaa aaaagcgttt aatccgggaa aggtgaatgg ataagatcga agttcggggc
     3661 gcccgcaccc ataatctcaa aaacatcaac ctcgttatcc cccgcgacaa gctcattgtc
     3721 gtgaccgggc tttcgggttc tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa
     3781 gggcagcgcc gttacgttga atccctttcc gcctacgcgc ggcagtttct gtcactgatg
     3841 gaaaagccgg acgtcgatca tattgagggg ctttctcctg ccatctcaat tgagcagaaa
     3901 tcgacgtctc ataacccgcg ttctacggtg gggacaatca ccgaaatcca cgactatttg
     3961 cgtttgttgt tcgcccgcgt cggcgaaccg cgctgtccgg accacgacgt cccgctggcg
     4021 gcgcaaaccg tcagccagat ggtggataac gtgctgtcgc agccggaagg caagcgtctg
     4081 atgctgctcg cgccaatcat taaagagcgc aaaggcgaac acaccaaaac gctggagaac
     4141 ctggcaagcc agggttacat ccgtgctcgt attgatggcg aagtctgcga tctttccgat
     4201 ccgccgaaac tggaactgca aaagaaacac actattgaag tggtggttga tcgcttcaag
     4261 gtgcgtgacg atcttaccca acgtcttgcg gagtcgtttg aaaccgcgct ggagctttcc
     4321 ggtggtacag cggtagtggc ggatatggac gacccgaaag cggaagagct gctgttctcc
     4381 gccaacttcg cctgcccaat ttgcggctac agtatgcgtg aactggagcc gcgactgttt
     4441 tcgtttaaca acccggcagg tgcctgcccg acctgtgacg gccttggcgt acagcaatat
     4501 ttcgatcctg accgcgtgat ccaaaatccc gagctgtcac tggctggcgg tgcgatccgt
     4561 ggctgggatc gccgcaactt ctattatttc cagatgctga aatcgctggc agatcactat
     4621 aagttcgacg tcgaagcgcc gtggggcagc ctgagcgcga acgtgcataa agtggtgttg
     4681 tacggttctg gcaaagaaaa cattgaattc aaatacatga acgatcgtgg cgatacctcc
     4741 attcgtcgtc atccgttcga aggcgtgctg cacaatatgg agcgccgtta taaagagacg
     4801 gaatccagtg cggtacgtga agaattagcc aagtttatca gcaatcgccc atgcgccagc
     4861 tgcgaaggaa cgcgtctgcg tcgggaagcg cgccacgtgt atgtcgagaa tacgccgctg
     4921 cctgctatct ccgacatgag catcggtcat gcgatggaat tcttcaacaa tctcaaactc
     4981 gcaggtcagc gggcgaagat tgcagaaaaa atccttaaag agatcggcga tcgtctgaaa
     5041 ttcctcgtta acgtcggcct gaattacctg acactttccc gctcggcaga aacactttcc
     5101 ggcggtgaag cccagcgtat ccgtctggcg agccagattg gtgcgggcct ggttggcgtt
     5161 atgtatgtac tggacgagcc gtctatcggc ctgcaccagc gcgataacga gcgcctgttg
     5221 ggtacgctta tccatctgcg cgatctcggt aataccgtga ttgtggtgga gcatgacgaa
     5281 gacgcaattc gcgccgctga ccatgtgatc gatatcggtc ctggtgcggg tgttcacggc
     5341 ggtgaagtgg tcgcagaagg tccgctggaa gcgattatgg cggtgcctga atcgttgacc
     5401 gggcagtaca tgagcggtaa acgcaagatt gaagtgccga agaaacgcgt tccggcgaat
     5461 ccagaaaaag tgctgaagct gacaggtgca cgcggcaaca acctgaagga cgtgacgctc
     5521 acgctgccag tcggtctgtt tacctgcatc acaggggttt caggttccgg taaatcgacg
     5581 ctgattaacg acacactgtt cccgattgcc caacgccagt tgaatggtgc gaccatcgcc
     5641 gaaccggcac cgtatcgcga tattcagggg ctggagcatt tcgataaagt gatcgatatc
     5701 gaccaaagcc caattggtcg tactccacgt tctaacccgg cgacctatac cggcgtgttt
     5761 acacctgtgc gcgaactgtt tgcgggcgta ccggaatccc gtgcgcgtgg ttatacgcca
     5821 ggacgtttca gctttaacgt ccgtggcggg cgctgcgaag cctgtcaggg cgacggtgtg
     5881 atcaaagtgg agatgcactt cctgccggat atctacgtgc cgtgcgatca gtgcaaaggt
     5941 aaacgctata accgtgaaac gctggaaatt aagtacaaag gcaaaaccat ccacgaagtg
     6001 ctggatatga ccatcgaaga ggcgcgtgag ttctttgatg cggtgccagc tctggcgcgt
     6061 aagctgcaaa cgttgatgga cgttggtctg acgtacattc gcctggggca gtccgcaacc
     6121 acactttctg gtggtgaagc ccagcgcgtg aagctggcgc gtgagctgtc aaaacgcggc
     6181 accgggcaga cgctgtatat tctcgacgag ccgaccaccg gtctgcactt cgccgatatt
     6241 cagcaactcc tcgacgtgct gcataaactg cgcgatcagg gcaataccat tgtggtgatt
     6301 gagcacaatc tcgacgtgat caaaaccgct gactggattg tcgacctggg accagaaggc
     6361 ggcagtggcg gcggcgaaat cctcgtctcc ggtacgccag aaaccgtcgc ggagtgcgaa
     6421 gcttcgcata cggcacgctt cctcaagccg atgctgtaat cgttaaggcc gctttctgag
     6481 cggccttttc ctttcagagt tgtaccagca atttacgctt ttcttccggt agtaaattca
     6541 ctgcctgctg ataagacgca tccaccagat aatagatttg cgaatccggc agcgaaccgt
     6601 cgagatacac ggtgctccag tgcgctttat tcagatggcg gctaggacgc acatcgctgt
     6661 gctgctgacg tagcagctcc gccagctccg ggctggtttt cagcgaaaca gctgggcgat
     6721 tttctacttc tttcaccatc gcaaacagta catcttccac tttgatctgc gtcgctttcc
     6781 agtcgttatg cacgctctgt tctgcgcctg gttttgccat gcaatattgt agcaactccg
     6841 aaatggtcat tttttactcc ccttgtagtg tcgcgatgat gcgacgcgat ccgccgtgga
     6901 tgcgatgttc ccccagccag atgccttgcc aggtgccggt ctgaatacgc cctttatgta
     6961 acggcaatac aagcgatgtt cccagcattg aggatttgat atgagaaggc atatcgtctg
     7021 ctccctcata gtcatgctca taatttccgt tgtcgggaac ggtgcggagg aaaaaacgct
     7081 ccatgtcgtg gcgtacggtg ggatcgcagt tctcattaag tgtcagagag gcggaggtat
     7141 gttgcagcaa cagatgcagt aagccgatgt taacgcgcgg catatcagcc agctgattca
     7201 gaatttcatc cgttaccaga tgaaacccac gagatttggc gctaagcgtg agcgtctttt
     7261 gataccacat gtgctgctcc ttgataaaac tctcttaatc agtttgcagc aagacagcga
     7321 aaggataaag gtgtgattaa aaaaacagca ttcgggagag catcacgctc tcccgctctg
     7381 tcagtattct gaattgacga tcacctcttc accaaacgca cccgcttgtg gcaagggttt
     7441 gtaggtagag ttggaggcgc gcagaatgcg gataccacga gcgccgacat cgcgtgcggc
     7501 ggtaatatcg ttatcggaat cgccataaaa aattcggata tttttatcct gcagccattg
     7561 cgattttgta ttttgccctg gtttatcgcc cgcaaagatt accggattca tgttggtggc
     7621 aggaatatga aaattatccg ccagcgtttt tgaaaccgtt tctgttttcg tcgggctacg
     7681 accagtcaca aagaagatcg cgtcaccgcg gcgtacatgc atatcaatca gctggcgagc
     7741 gacctctttt ggaatgctga attcatccca gccattattc attttttccc agaacacagg
     7801 atttttcaga taatcttcgc tttctggcga gaaggttttt ttgccgcgcc agaaccccgg
     7861 actggaaaaa agtaccgtgt catcgatatc aaaccccacc gccattggcg gacgccctgc
     7921 gaggctattt tcaatttgtg cgaccgaaac ccaatgaatt ggtgcctgtt cagcaagcct
     7981 ggcaacgtta gtcccagggt taagcggtga aggagatgag gccagggcaa cagcggaact
     8041 gtttagagcg aacaataagc aaacggcact gattgcctgt gtgatcttgc gcatattttt
     8101 ccctaaatag tcagtatgtt gaaacttttt attgtgagat ttgttgcaaa aaccatctga
     8161 ccataacgcc agctgccggg gaaaggaagg attatctgcg gtttttgtga tgaaaagcag
     8221 gatgattata tttaacccag taaatacgtt cctgtctttt tttgtataaa aaatgtttat
     8281 tactcgatgt aaggatacat taattttacg tttattatga tgaatcctct gcaaatgagc
     8341 gaaatttgaa agaacattta tctgctgtac gatttaatga acttaatgat acagcattac
     8401 aaaaacaatc gaagtttata aagatgattc ctgattgacc agaaccctat cctacaatgg
     8461 tgactaattt tattactccc gatggagatg atgatatgaa tatatcatat gtgaatagca
     8521 acaaaacgac atctttacct gttgagctag atgcactaaa taataaagac at
//
LOCUS       DAZRVY010000159         8452 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZRVY010000159 DAZRVY010000000
VERSION     DAZRVY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.159"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHXOX_003721"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHXOX_003721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR7007086.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXOX_003722"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXOX_003722"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR7007087.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXOX_003723"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXOX_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR7007088.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXOX_003724"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXOX_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR7007089.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHXOX_003725"
     CDS             complement(4036..5568)
                     /locus_tag="ACHXOX_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR7007090.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXOX_003726"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXOX_003726"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR7007091.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXOX_003727"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXOX_003727"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR7007092.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
BASE COUNT         2115 a         2129 c         2268 g         1940 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aa
//
LOCUS       DAZRVY010000160         8182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZRVY010000160 DAZRVY010000000
VERSION     DAZRVY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.160"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHXOX_003728"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHXOX_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR7007093.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHXOX_003729"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHXOX_003729"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR7007094.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHXOX_003730"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHXOX_003730"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR7007095.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHXOX_003731"
     CDS             6679..>8182
                     /locus_tag="ACHXOX_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR7007096.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZRVY010000161         8157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZRVY010000161 DAZRVY010000000
VERSION     DAZRVY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.161"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHXOX_003732"
     CDS             <1..254
                     /locus_tag="ACHXOX_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR7007097.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHXOX_003733"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHXOX_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR7007098.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHXOX_003734"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHXOX_003734"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR7007099.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHXOX_003735"
     CDS             complement(2905..3180)
                     /locus_tag="ACHXOX_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR7007100.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHXOX_003736"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHXOX_003736"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR7007101.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHXOX_003737"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHXOX_003737"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR7007102.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHXOX_003738"
     CDS             5140..5466
                     /locus_tag="ACHXOX_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR7007103.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHXOX_003739"
     CDS             5650..6369
                     /locus_tag="ACHXOX_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR7007104.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHXOX_003740"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHXOX_003740"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR7007105.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHXOX_003741"
     CDS             7708..>8157
                     /locus_tag="ACHXOX_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR7007106.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZRVY010000162         8051 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZRVY010000162 DAZRVY010000000
VERSION     DAZRVY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.162"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1341
                     /gene="yjeM"
                     /locus_tag="ACHXOX_003742"
     CDS             <1..1341
                     /gene="yjeM"
                     /locus_tag="ACHXOX_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR7007107.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="ACHXOX_003743"
     CDS             complement(1492..4815)
                     /gene="mscM"
                     /locus_tag="ACHXOX_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR7007108.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4837..5805)
                     /gene="asd"
                     /locus_tag="ACHXOX_003744"
     CDS             complement(4837..5805)
                     /gene="asd"
                     /locus_tag="ACHXOX_003744"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR7007109.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="ACHXOX_003745"
     CDS             complement(5902..6954)
                     /gene="rsgA"
                     /locus_tag="ACHXOX_003745"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR7007110.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7049..7594
                     /gene="orn"
                     /locus_tag="ACHXOX_003746"
     CDS             7049..7594
                     /gene="orn"
                     /locus_tag="ACHXOX_003746"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR7007111.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7805..7880
                     /locus_tag="ACHXOX_003747"
     tRNA            7805..7880
                     /locus_tag="ACHXOX_003747"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7838..7840,aa:Gly,seq:gcc)
BASE COUNT         1855 a         2084 c         2168 g         1944 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagcg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgacgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct ttgctattca
      841 ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc
      901 ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc
      961 ttcatcctgc tggtttcgtt tggtggcggt accgcatccg cgttctttaa caagctgacg
     1021 ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccattc
     1081 tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca
     1141 atgattgcga tggtggttgt cgtactggtg gtgacatttg cgaacgtctt caccattatt
     1201 cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc
     1261 ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa
     1321 cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg
     1381 agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg
     1441 atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact
     1501 tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag
     1561 gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg catggaagcc
     1621 agccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc
     1681 ataaatacgc agctcgaaaa tctgaatccc ttgttgcaga tccaccagga agacttccgg
     1741 tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac
     1801 ttcttcactg ttggcatcag caggggccgg aatcgtcaac actacgcgcg tgaccgagtc
     1861 agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc
     1921 tttacggtcc cagtcgctga tggtggtggc gcgggtgtta attttcgtca cgctaccggt
     1981 gagatcgcga attgtcaccg tatcgccaat gcgaatcggt ttttcgaaca ggataatcag
     2041 accagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc
     2101 ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc cgaccagccc
     2161 gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtagcccg tacccggcgt
     2221 taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg
     2281 cgtggtgatg ataaacacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag
     2341 actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc
     2401 gaaagcggaa tggatttctg accacagcac aatgacagaa agcagggcga tcaacatcaa
     2461 aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc
     2521 atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc
     2581 acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcggcg
     2641 ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc
     2701 aaccgaggtt tcaagcctcg ctaacagcgc ctgtgccgtt gccagataac ccacagccga
     2761 cgccagaatg gcaaccaacg gcgcgccaat catcatgttc cacagcatat ggttggtaat
     2821 gttgtcgccg ctgccttctt tgttgagata cagcggaatc ccggcctttt tcaggctgag
     2881 ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccttccca gcgaaccgga
     2941 gaattcacgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg gcacaataag
     3001 cccgatgctc atcaggtagt agcgcatccc acgagatacg cgttcgcgcg gccagccaaa
     3061 atgagcgata aacaagccgt tcgggcgggc aaaggtggcg caaatcatca ctacccacag
     3121 cagcggcacg gtggccgtta caccatcgcc aatcgcgacc gccagcggat aaggccacgc
     3181 ctcgcgcaag ccgtaaccca gcgtcatcca cagcaccggt aacggtgacg cgacgagaat
     3241 cgaccagaaa agagtgcgca acgtcagcca gaagtgatcc tgagtcactt tgccaacttt
     3301 cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac tgcaaccgac
     3361 cagaatcaac gcgccaaaca gcggcaaaat tgtctctttg ctggtcagca tcatcacact
     3421 ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat cctgggcgat
     3481 ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca gatagcggtg
     3541 cgttgcttcg ttcacctctt tcagcgcatc ctccagttgc ccgttggaga ctttcagctt
     3601 ggtcagttcc aatagtagcg tgtcgccacc ctgcaacaat gagttcagca actcacgctg
     3661 agtgcgcagt tgtgcttcca gaatacggtt ttgctcggcg gtcagcggct gaccgtcggc
     3721 ctgatggatt tgccgtagca gcggctgttt attaagcaga tcctcataac gtaaccgttg
     3781 cacacgcaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt ctggtagccg
     3841 tgccacctgc gctcgcaacg cttcaccgag cagattggac gatcccagcc attgcgactg
     3901 ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag cctgacgctg
     3961 ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agcgctgccg atagttcgcg
     4021 gttaattttg aattgcgcga cgatatcttt cggcaaatcg gcactgtttt ctgccagtaa
     4081 ttcggtactt tccagcgccc gctccgcctc aagctgacgt tggctattca actgattacg
     4141 taaggcctgc aaatacgcat ccagttgctg gctctctttt tccgcaagct ctgagcgtaa
     4201 gcgcgctaat tcttggcggt tattggcaga cagctgcgcc agctccagtt catcaacgag
     4261 cgccttaaga cgtgcagagt cagactgcaa cgcgaaattt tgtgcctgat tgagcggagt
     4321 attgccggta agcgttccca ggcggcgctc gatctcattt aactgacggc gggcgtcggt
     4381 ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct cctgctcttg
     4441 ctgggcctga cggcttttat ccagcaactg gctgctgacc tggagaattt cctgattcag
     4501 cgcgtcggta gacattcccg gcgacacgct gcgcggcttg tcacgcatgt tgtttaattg
     4561 tgcgcgcaga gtagcggaga gtttcggata attatcgata acttgctgat attgtttgat
     4621 gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgaa gcgcctctac
     4681 gacttccggc tgtgcgggtt tcgccgcttt tgcctgctcc agttcctgag tgatttgttt
     4741 gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca tcagaaaagt
     4801 gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt ttttgtcgtc
     4861 aaccaatggg ctggcgtcgt gttctgcttc gatctcttca gcaggaagcg gggcaggttc
     4921 agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggcaaa ttttcgtgac
     4981 agacaggctt tccagttgct caaccagatt cactttaccc ggtgcaaaca ggttgataac
     5041 ggtggaaccg agtttaaagc gacccatttc ctggcctttc agcagtgcca cagaaccgtc
     5101 gttttccccg gcaggccagg tccagcgctt gatgatacct tcgcgcggcg gcgtaatggt
     5161 gcccgcccag accgtctcaa tgctgccaac aatcgtcgct ccgaccagaa tctgcgccat
     5221 tgggccaaat tcggtatcga aaaggcaaat cacgcgttcg ttacgggcaa acagattcgg
     5281 cacgttctga gccgtgagat ggttaacgga gaagagatcg cccggcacgt agatcatctc
     5341 acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgcgggg agaggtaagt
     5401 ggtcacaaac gtaccgttgc ggaacaggtc cgccatcaga tagttgcctg ccagcagggc
     5461 ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga ttttacccaa
     5521 ctggctgata acgccatcgg caggcatgac cagtacattc ggatcggtat cgattgggcg
     5581 tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc tggcggtgtc
     5641 cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat cgataaccag
     5701 ttttgtcaac catcctgccc gcttgcttgc gccccaaccc gccaggcgag taagccatag
     5761 tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag cctccaggcc
     5821 attgttttgt cgttcctgat tcggcctaca tgccggatcc tgaaaaaaag gggacgattc
     5881 taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac gcgtttttac
     5941 ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg cgattttccc
     6001 ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc aatcgcgata
     6061 tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt gttccggctc
     6121 caggtgccag aggccgaact cacgcacacc aggggaatca atcacatcac cgccgtgcgg
     6181 gaagtgatac agccgagcgg cggtcgtggt gtgctgacca agccccgagt tgtcagagac
     6241 atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc tggatttgcc
     6301 gacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt ccagcggttt
     6361 tagcccatcc tgagtatggc tggaaaccat caatacgcga taaccgatat tgcggtagat
     6421 atccatctgc tcgttgacga acgccatgcc ttcgtcgtcc agcaggtcta tcttgttgag
     6481 cacaataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt cgataatatt
     6541 gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt tggcggcaat
     6601 aggttttacg ccgtcgtaga aatccggacg cgtcaacacc gaggtacgct catgcaccgc
     6661 ttccacgatc cctttgacat ttacgccttc cgccgccggt ttacccggac gccagactac
     6721 gcggtcgccg gttaccagcg aacggatggt acggcgaata ttgcagcggt gaacgtcgcc
     6781 atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac cttcatcagg
     6841 ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa gacgacgctg
     6901 gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac tcaatcgtac
     6961 tgactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg tggatcaata
     7021 tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc tgatttggat
     7081 cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga ttgccacgct
     7141 ggtgaccgat gccaatctga atattctggc agaagggccg accattgcag tacatcagtc
     7201 cgatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg ccagcgggct
     7261 ggtagagcgc gtgaaagcga gcacgatggg cgatcgggaa gctgaactgg caacgctcga
     7321 attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgcggtaaca gcatcggaca
     7381 ggaccgtcgt ttcctgttta aatacatgcc ggagctggaa gcctacttcc actaccgtta
     7441 tctcgatgtc agcaccctga aagagctggc gcgccgctgg aagccggaaa ttctggatgg
     7501 ttttaccaag caggggacgc atcaggcaat ggatgatatc cgtgaatcgg tggcggagct
     7561 ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac gtaagcgccg
     7621 gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg ataatttcag
     7681 cacttgagat aaaaacgcaa aaaaaacttt tttggggggt tgcagaggga aagatttctc
     7741 gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca aaattaagta
     7801 gtacgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg tcgcgagttc
     7861 gagtctcgtt tcccgctcca aaatttgaaa agtgctgcaa agcacagacc acccaatcgc
     7921 agtatttcga attgcgacaa ggcggcaact gaacgaatcc ttgggaactt acaaaagtat
     7981 gtgactaagg tgagtgaagg cagccaaggc agtagcggtt cgaaagacga agattaagcg
     8041 ggaatagctc a
//
LOCUS       DAZRVY010000163         8018 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZRVY010000163 DAZRVY010000000
VERSION     DAZRVY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.163"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHXOX_003748"
     CDS             complement(152..487)
                     /locus_tag="ACHXOX_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR7007112.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHXOX_003749"
     CDS             complement(491..835)
                     /locus_tag="ACHXOX_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR7007113.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHXOX_003750"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHXOX_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR7007114.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHXOX_003751"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHXOX_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR7007115.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHXOX_003752"
     CDS             1257..1376
                     /locus_tag="ACHXOX_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR7007116.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHXOX_003753"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHXOX_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR7007117.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHXOX_003754"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHXOX_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR7007118.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHXOX_003755"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHXOX_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR7007119.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHXOX_003756"
     CDS             4176..4544
                     /locus_tag="ACHXOX_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR7007120.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHXOX_003757"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHXOX_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR7007121.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHXOX_003758"
     CDS             5295..5588
                     /locus_tag="ACHXOX_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR7007122.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHXOX_003759"
     CDS             5660..6319
                     /locus_tag="ACHXOX_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR7007123.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHXOX_003760"
     CDS             6411..6857
                     /locus_tag="ACHXOX_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR7007124.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHXOX_003761"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHXOX_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR7007125.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         2067 a         1799 c         1840 g         2312 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt taaatatata aattgtaatg aaactcct
//
LOCUS       DAZRVY010000164         7992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZRVY010000164 DAZRVY010000000
VERSION     DAZRVY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.164"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(283..1314)
                     /gene="rsmC"
                     /locus_tag="ACHXOX_003762"
     CDS             complement(283..1314)
                     /gene="rsmC"
                     /locus_tag="ACHXOX_003762"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR7007126.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1417..1830
                     /gene="holD"
                     /locus_tag="ACHXOX_003763"
     CDS             1417..1830
                     /gene="holD"
                     /locus_tag="ACHXOX_003763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR7007127.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1799..2245
                     /gene="rimI"
                     /locus_tag="ACHXOX_003764"
     CDS             1799..2245
                     /gene="rimI"
                     /locus_tag="ACHXOX_003764"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR7007128.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2260..2937
                     /gene="yjjG"
                     /locus_tag="ACHXOX_003765"
     CDS             2260..2937
                     /gene="yjjG"
                     /locus_tag="ACHXOX_003765"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR7007129.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3028..4617
                     /gene="prfC"
                     /locus_tag="ACHXOX_003766"
     CDS             3028..4617
                     /gene="prfC"
                     /locus_tag="ACHXOX_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR7007130.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5010..5615
                     /gene="osmY"
                     /locus_tag="ACHXOX_003767"
     CDS             5010..5615
                     /gene="osmY"
                     /locus_tag="ACHXOX_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR7007131.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5742..5903
                     /locus_tag="ACHXOX_003768"
     CDS             5742..5903
                     /locus_tag="ACHXOX_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR7007132.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6025..7098
                     /locus_tag="ACHXOX_003769"
     CDS             6025..7098
                     /locus_tag="ACHXOX_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR7007133.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7095..7877
                     /locus_tag="ACHXOX_003770"
     CDS             7095..7877
                     /locus_tag="ACHXOX_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7007134.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1905 a         2064 c         2168 g         1855 t
ORIGIN      
        1 ttccgccacc ttcgcatacc atcaattctt aaaaagaatt gctaccacgg aggcgcattc
       61 tagtggtttt cagcttttcg tcaatagtta attatcgccg gaggtgtaat tgctggaaaa
      121 atgtccatca ggaaactagc gtgcaggttt ggtaggcatg cgggggcaga tgccagatgc
      181 gacgctggcg cgtcttatct ggcctacgaa gggctaacgt gcaggttttg taggtcggat
      241 aaggcgttca cgccgcatcc gacacggtat tcggcgagat aattaacctt tcttcgcctg
      301 gcgggtcata atggcgcgat acaccttgaa acgcccggtt tgcgcaatca cttcatggaa
      361 gccaaatgtc tcatccagca cgtccgggta aggcaggaag gcgttcgcta caattcgcag
      421 ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag
      481 gctggtttgc atcccatcgt ggaacggcgg gttggagatg atcatatcaa aacgaccttt
      541 cacctcggaa aagacgttgc tggcaaagac ttcaccttca acacagttgg ccgcaagtgt
      601 tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac aaggtgagac gaattttcgg
      661 cgaatggcgc gcaaaggcaa ctgaaagcac ccccgcgcca cagccgacat ccagcacttt
      721 acctttcgtg tgcggagtta acgtcgagag cagcaactgg ctaccgacat ccagaccgtc
      781 gcggctaaac acgccaggca gcgttttgac cgtcaggcca tcgacgctgt attcgcccca
      841 gaatttatcg gcatcaaata ccggctgttt ttccagacga ccaaaataga ggccacagcg
      901 acgagcgctg tcgactttat tcaacggcgc ataatctgcc agcatctgct cggcgctgcg
      961 cacgccgctg cggttctcgc caacgacaaa aatatctgtc cccactggca gcagagaaag
     1021 taaattcatc aactggaact gggcttccgg tttgttcttc ggccagtagt aaatcagcgt
     1081 atcgcaatct gcgacgtcat ccaccgtggc gaccagactg aaacgggcgt tatcccccat
     1141 ctggcggctt aatacctgcc agtggtggaa ttgctgggta tgagcacggc tggccgcggt
     1201 atctaaacgc gcgggcaggt catcctgtaa gtctccggca aacagaatac ggctttgttc
     1261 gaaatcatca ctgtgacgca gcaagacttc acttgccggg gtaaatgcag acatggaatg
     1321 ctcctcaatt gatactggcg gcgattatag ccatatgttg gcgcggtatc gacgaatttg
     1381 ctatatttgc gcccctgaca acaggagcga ttcgctatga catcccgacg agactggcag
     1441 ttacagcaac tgggcattac ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggag
     1501 attgccattg cgatcccggc acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc
     1561 ctgaccgatc ctttagtgag cgatgttctg cgcgcattaa ccgtcagccc cgatcaagtg
     1621 ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg
     1681 cggttgggta ctgacgaacc gctatcactg gaaggcgctc aggtggcatc accggcgctc
     1741 accgaattac gggcaaaccc aacggcacgc gccgcgttat ggcaacagat ttgcacatat
     1801 gaacacgatt tcttccctcg aaacgactga tttaccagcg gcttaccaca ttgaacaacg
     1861 cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc aaccagggcg agcgttatct
     1921 caactttcag ttaacgcaaa acggcaaaat ggcggcgttt gcgattacgc aagtggtgct
     1981 ggatgaagct acattgttca atattgcggt cgatcctgac tatcagcgtc agggattggg
     2041 aagggtgctg ctggaacatc tgatcgacga actggaaaaa cgcggcgtgg cgacactatg
     2101 gctggaagtc cgtgcctcaa atgctgccgc cattgccctg tacgaaagtt taggctttaa
     2161 cgaggcgacg attcgccgca attactaccc caccacggac ggtcgcgaag acgccatcat
     2221 catggcgttg ccaatcagta tgtaagacaa ggtggaataa tgaagtggga ctggattttc
     2281 tttgatgccg atgaaacgct gtttaccttt gactcattca ccggcctgca gcggatgttt
     2341 cttgattaca gcgtcacctt taccgctgaa gattttcagg actatcaggc cgttaacaag
     2401 ccactgtggg tggattatca aaacggcgcg atcacttcat tacagcttca gcacgggcgt
     2461 tttgagagct gggccgaacg gctgaaagtt gaagcaggct tgcttaacga tgcctttatt
     2521 aatgcgatgg cggaaatctg cacgccgctg ccgggcgcgg tttctctgct taacgccatt
     2581 cgtggcaacg ccaaaatcgg catcatcacc aacggcttta gcgccttgca gcaagtgcgt
     2641 ctggaacgca cgggcctgcg tgattacttc gatttgctgg tgatttccga agaagttggc
     2701 gttgccaaac cgaataagaa aattttcgat tatgcgctgg aacaggcggg caatcctgac
     2761 cgttcacgcg tgctgatggt tggcgacact gccgagtccg atattctcgg tggcatcaac
     2821 gccgggcttg cgacctgctg gctgaatgcg cacaatcgcg agcaaccaga aggcatcgcg
     2881 cccacctgga ccgtttcttc gttgcacgaa ctggagcagc tcctgtgtaa acactgattg
     2941 cctccccccc gttgatgggt aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg
     3001 atgccgctta ctcaagaaga aagaattatg acgttgtctc cttatttgca agaggtggcg
     3061 aagcgccgca cttttgccat tatttctcac ccggatgccg gtaagactac catcaccgag
     3121 aaggtgctgc tgttcggaca ggccattcag accgccggta cagtaaaagg ccgtggttcc
     3181 aaccagcacg ctaagtcgga ctggatggag atggaaaagc agcgtgggat ctccattact
     3241 acgtctgtga tgcagtttcc gtatcacgat tgcctggtta acctgctcga caccccgggg
     3301 cacgaagact tctcggaaga tacctatcgt accctgacgg cggtggactg ctgcctgatg
     3361 gttatcgacg ctgcaaaagg tgttgaagat cgtacccgta agctgatgga agttacccgt
     3421 ctgcgcgaca cgccgatcct cacctttatg aacaaacttg accgtgatat ccgcgacccg
     3481 atggaactgc tcgatgaagt tgagaacgag ctgaaaatcg gctgcgcacc gatcacctgg
     3541 ccgattggct gcggcaagct gtttaaaggc gtttaccacc tttataaaga cgaaacctat
     3601 ctctatcaga gcggtaaagg ccacacgatt caggaagtcc gtattgttaa agggctgaat
     3661 aacccggatc tcgacgctgc ggttggtgaa gatctggcac agcagctacg tgacgaactg
     3721 gaactggtga aaggcgcgtc taacgagttc gacaaagaat tgttccttgc gggcgaaatc
     3781 actccggtat tcttcggtac tgcgctgggt aacttcggcg ttgatcatat gctggatggc
     3841 ctggtggagt gggctcctgc gccgatgccg cgtcagactg atacccgtac cgtagaggcg
     3901 agcgaagaca aatttaccgg cttcgtattt aaaattcagg ccaacatgga cccgaaacac
     3961 cgcgaccgcg tggcgtttat gcgcgtagta tccggtaaat atgaaaaagg catgaagctg
     4021 cgccaggtgc gtactgcgaa agatgtggtt atctccgacg cgctgacctt tatggcgggc
     4081 gaccgttcgc acgttgaaga agcgtatccg ggcgatatcc tcggcctgca caaccacggc
     4141 actattcaga tcggcgacac ctttacccag ggtgagatga tgaagttcac cggtattccg
     4201 aacttcgcac cagaactgtt ccgtcgtatc cgcctgaaag atccgctgaa gcaaaaacag
     4261 ctgctcaaag ggctggtaca gctttccgaa gagggcgcgg tgcaggtgtt ccgtccgatc
     4321 tccaacaacg atttgatcgt tggtgctgtt ggtgtgctgc agtttgatgt ggtggtatcg
     4381 cgcctgaaga gcgaatacag cgttgaagca gtatatgaat cagtcaacgt tgccactgcc
     4441 cgctgggtag aatgtgcgga cgcgaagaaa ttcgaagagt tcaagcgtaa gaacgaaagc
     4501 caactggcgc ttgatggcgg cgataacctc gcttacatcg ctaccagcat ggtcaacctg
     4561 cgcctggcgc aggaacgtta tcccgacgtt cagttccacc agacccgcga gcattaattc
     4621 ttgcctccag ggcgcggtag ccgctgcgcc ctgtcaattt cccttcctta ttagccgctt
     4681 acggaatgtt cttaaaacat tcacttttgc ttatgttttc gctgatatcc cgagcggttt
     4741 caaaattgtg atctatattt aacaaagtaa tgacatttct gacggcgtta aataccgttc
     4801 aatgcgtaga tatcagtatc taaagccgtc gattgtcatt ctaccgatat taataactga
     4861 ttcagaggct gtaatggtcg ttattcatca cccatcgctt ttgtgatggc gaccattgac
     4921 ttctgtagag ggtgaagtct ctccctattc agcaatgcaa cctcgtgttg ccaggctcaa
     4981 attacgagca aacatacagg aataaatcga tgactatgac aagactgaag atttcgaaaa
     5041 ctctgctggc tgtaatgttg acctctgccg tcgcgaccgg ctctgcctac gcggaaaaca
     5101 acgcacagac taccaatgaa agcgcagggc aaaaagtcga tagctctatg aataaagtcg
     5161 gtaatttcat ggatgacagc gccatcaccg cgaaagtgaa ggcggcgctg gtggatcatg
     5221 acaacatcaa gagcaccgat atctctgtca aaaccgatca aaaagtcgtg accctgagcg
     5281 gtttcgttga aagccaggct caggccgaag aggcagtgaa agtggcgaaa ggcgttgaag
     5341 gggtgacctc tgtcagcgac aaactgcacg ttcgcgacgc taaagaaggc tctgtgaagg
     5401 gctacgcggg tgacaccgcc accaccagtg aaatcaaagc caaactgctg gcggacgata
     5461 tcgtcccttc ccgtcatgtg aaagttgaaa ccaccgacgg cgtggttcag ctctccggta
     5521 ccgtcgattc ccaggcacag agcgaccgtg ctgaaagtat cgccaaagcg gtagatggtg
     5581 tgaaaagcgt taaaaatgat ctgaaaacta agtaattcgt cgtaattcgt cctcccgaac
     5641 tttgtcggga ggcgcgatgt gcaccacact aaaaatatcg cattgagtga ttttcacgct
     5701 catatcaagc ggtgacatta actatggtaa aggagacgct tatgtttcgt tggggcatca
     5761 tatttctggt tatcgcgtta atcgccgccg cacttgggtt tggtggtctg gccggtaccg
     5821 ctgcaggcgc agctaaaatt gtctttgtcg tcgggattat tctgttcctg gtgagtttgt
     5881 tcatgggccg aaaacgaccc tagatttcag accattaaga aaattttatc caaagccagt
     5941 ccagcggact ggcttttgcg gttttagcga ataataaatt gcgttacttt gtcattcttt
     6001 cacaacaaaa acaggaaggc agaggtgggg cagcgaatac ctgtaacgct tggtaatatt
     6061 gcgccgttgt cgctaaggcc gttccagcct ggacgaatag ctctggtgtg cgaaggcggc
     6121 ggacagcgtg gaattttcac ggctggcgtg ctggatgagt ttatgcgcgc gcagtttaat
     6181 cctttcgatc tttatctcgg cacatctgcc ggggcgcaga acctctcggc gtttatctgc
     6241 aatcagcccg gttacgcgcg caaagtcatc atgcgctata ccacaaaacg cgaatttttc
     6301 gatccattgc gctttgtccg tggaggaaat cttatcgatc tcgactggct ggtggaggcc
     6361 accgcaagcc agatgccgtt gcaaatggat accgccgcgc ggttgtttga cagcggcaaa
     6421 tcgttttata tgtgcgcctg tcgtcaggat gactacgcgc ctaattactt tttaccaacc
     6481 aaacaaaact ggctggatgt gattcgcgcc tccagtgcga tacctggctt ttatcgtagc
     6541 ggagtgtcgc tggaaggcat taactacctg gatggcggga tcagtgatgc gattccggtt
     6601 aaagaggcgg caaggcaggg cgctaaaacg ttggtcgtca ttcgcactgt gccgtcacaa
     6661 atgtactaca cgccgcagtg gttcaaacgc atggaacgct ggctgggtga cagtagcctg
     6721 cagccgctgg tcaatctggt gcagcatcat gaaaccagct atcgtgacat tcagcaattt
     6781 attgagaaac caccgggcaa gctgcggata ttcgaaattt atccgccgaa gccattacat
     6841 agtatcgcgc ttggcagtcg gattccggcg ctgcgtgaag actataaact tgggcgttta
     6901 tgcggtcgtt atttcctcgc cacggttggc aagctattaa ctgaaaaagc gccgcttacc
     6961 cgccatctgg tgccagtggt gacgccggaa tcgatcgtta ttccgcctgc gccagtcgcc
     7021 aacgatacgc tggttgccga agtgagcgat gctccgcagg cgaacgaccc gacatttaac
     7081 aatgaggatc tggcttgatt tgccgtttta tcgataccca ctgccatttt gatttcccgc
     7141 cgtttagtgg cgatgaagag gccagcctgc aacgcgcggc acaagcgggc gtaggcaaga
     7201 tcattgttcc ggcgacagag gcggaaaatt ttgcccgcgt gcaggcgtta gcggaaaaat
     7261 atcaaccgct gtatgccgca ttggggttgc atccggggat gttggaaaaa catagcgatg
     7321 tgtctcttga tcagctacag caggcgctgg aaaggcgtcc ggcgaaggtg gtggcggtgg
     7381 gggagatcgg tctggatctc ttcggcgacg atccgcaatt tgagaggcag cagtggttac
     7441 tcgacgaaca gctgaaactg gcgaaacgct acgatctgcc ggtgatcctt cattcccggc
     7501 gtatacacga caaactggcg atgcatctta aacgtcacga tttaccgtgc actggcgtgg
     7561 ttcacggttt ttccggcagc ctgcaacagg ccgagcggtt tgtacagctg ggctacaaaa
     7621 tcggcgtagg cggtactatc acctatccac gcgccagtaa aacccgcgat gtcatcgcaa
     7681 aattaccgct ggcatcgttg ttgctggaaa ccgacgcgcc ggatatgccg ctcaacggct
     7741 ttcaggggca gcctaaccga ccggaacagg ctgtgcgggt gtttgacgtg ctttgcgagt
     7801 tgcgcccgga accagaagat gagattgccg aagtgttgct taataatacg tatgcgttgt
     7861 ttagcgttag tgggtagcct acccttcgta ggcagcatcc ggcagtcggc gcataatgcc
     7921 tgatgcgacg cttaacgcgt cttatcatgc ctacaggttt gtgccgaacc gtaggccgga
     7981 taaggcgttc ac
//
LOCUS       DAZRVY010000165         7953 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZRVY010000165 DAZRVY010000000
VERSION     DAZRVY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.165"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHXOX_003771"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHXOX_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR7007135.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHXOX_003772"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHXOX_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR7007136.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHXOX_003773"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHXOX_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR7007137.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHXOX_003774"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHXOX_003774"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR7007138.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHXOX_003775"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHXOX_003775"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR7007139.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHXOX_003776"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHXOX_003776"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR7007140.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHXOX_003777"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHXOX_003777"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR7007141.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPYSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHXOX_003778"
     CDS             5315..6439
                     /locus_tag="ACHXOX_003778"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR7007142.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHXOX_003779"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHXOX_003779"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR7007143.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>7953
                     /locus_tag="ACHXOX_003780"
     CDS             7245..>7953
                     /locus_tag="ACHXOX_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR7007144.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFID"
BASE COUNT         2024 a         1836 c         2034 g         2059 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctactccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atg
//
LOCUS       DAZRVY010000166         7946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZRVY010000166 DAZRVY010000000
VERSION     DAZRVY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.166"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHXOX_003781"
     CDS             <1..359
                     /locus_tag="ACHXOX_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR7007145.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHXOX_003782"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHXOX_003782"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR7007146.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHXOX_003783"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHXOX_003783"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR7007147.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="ACHXOX_003784"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="ACHXOX_003784"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR7007148.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="ACHXOX_003785"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="ACHXOX_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR7007149.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHXOX_003786"
     CDS             complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHXOX_003786"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR7007150.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHXOX_003787"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHXOX_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR7007151.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2031 a         2085 c         1888 g         1942 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgctttagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgaca
//
LOCUS       DAZRVY010000167         7938 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZRVY010000167 DAZRVY010000000
VERSION     DAZRVY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.167"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHXOX_003788"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHXOX_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHXOX_003789"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHXOX_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR7007152.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHXOX_003790"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHXOX_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR7007153.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHXOX_003791"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHXOX_003791"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR7007154.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHXOX_003792"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHXOX_003792"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR7007155.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHXOX_003793"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHXOX_003793"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR7007156.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHXOX_003794"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHXOX_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR7007157.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1834 a         2054 c         2073 g         1977 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaa
//
LOCUS       DAZRVY010000168         7774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZRVY010000168 DAZRVY010000000
VERSION     DAZRVY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.168"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHXOX_003795"
     CDS             complement(<1..1780)
                     /locus_tag="ACHXOX_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR7007158.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHXOX_003796"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHXOX_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR7007159.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHXOX_003797"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHXOX_003797"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR7007160.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHXOX_003798"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHXOX_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR7007161.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHXOX_003799"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHXOX_003799"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR7007162.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHXOX_003800"
     CDS             complement(5968..7005)
                     /locus_tag="ACHXOX_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR7007163.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHXOX_003801"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHXOX_003801"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR7007164.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZRVY010000169         7715 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZRVY010000169 DAZRVY010000000
VERSION     DAZRVY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.169"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHXOX_003802"
     CDS             <1..643
                     /locus_tag="ACHXOX_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7007165.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHXOX_003803"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHXOX_003803"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR7007166.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLTVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHXOX_003804"
     CDS             2474..2986
                     /locus_tag="ACHXOX_003804"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR7007167.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHXOX_003805"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHXOX_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR7007168.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHXOX_003806"
     CDS             5579..5782
                     /locus_tag="ACHXOX_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR7007169.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHXOX_003807"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHXOX_003807"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR7007170.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHXOX_003808"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHXOX_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR7007171.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1783 a         2103 c         2021 g         1808 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg acagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZRVY010000170         7708 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZRVY010000170 DAZRVY010000000
VERSION     DAZRVY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.170"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHXOX_003809"
     CDS             complement(<1..304)
                     /locus_tag="ACHXOX_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR7007172.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="ACHXOX_003810"
     CDS             complement(1125..1337)
                     /locus_tag="ACHXOX_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR7007173.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="ACHXOX_003811"
     CDS             complement(1413..2030)
                     /locus_tag="ACHXOX_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR7007174.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="ACHXOX_003812"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="ACHXOX_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR7007175.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="ACHXOX_003813"
     CDS             complement(3911..4972)
                     /locus_tag="ACHXOX_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR7007176.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHXOX_003814"
     CDS             5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHXOX_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR7007177.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHXOX_003815"
     CDS             complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHXOX_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR7007178.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1962 a         1801 c         1857 g         2088 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttgtc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agccccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtatgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgcctg gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 tcccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtgag
//
LOCUS       DAZRVY010000171         7672 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZRVY010000171 DAZRVY010000000
VERSION     DAZRVY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.171"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            136..639
                     /gene="dps"
                     /locus_tag="ACHXOX_003816"
                     /gene_synonym="pexB"
     CDS             136..639
                     /gene="dps"
                     /locus_tag="ACHXOX_003816"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR7007179.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1043..1789
                     /gene="glnH"
                     /locus_tag="ACHXOX_003817"
     CDS             1043..1789
                     /gene="glnH"
                     /locus_tag="ACHXOX_003817"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR7007180.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1928..2587
                     /gene="glnP"
                     /locus_tag="ACHXOX_003818"
     CDS             1928..2587
                     /gene="glnP"
                     /locus_tag="ACHXOX_003818"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR7007181.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2584..3306
                     /gene="glnQ"
                     /locus_tag="ACHXOX_003819"
     CDS             2584..3306
                     /gene="glnQ"
                     /locus_tag="ACHXOX_003819"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR7007182.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3423..5648
                     /gene="ybiO"
                     /locus_tag="ACHXOX_003820"
     CDS             3423..5648
                     /gene="ybiO"
                     /locus_tag="ACHXOX_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR7007183.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5645..6571)
                     /gene="rlmF"
                     /locus_tag="ACHXOX_003821"
     CDS             complement(5645..6571)
                     /gene="rlmF"
                     /locus_tag="ACHXOX_003821"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR7007184.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6847..7107
                     /gene="mcbA"
                     /locus_tag="ACHXOX_003822"
     CDS             6847..7107
                     /gene="mcbA"
                     /locus_tag="ACHXOX_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR7007185.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7372..>7672
                     /locus_tag="ACHXOX_003823"
     CDS             7372..>7672
                     /locus_tag="ACHXOX_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR7007186.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1853 a         1964 c         1934 g         1921 t
ORIGIN      
        1 tctttattag ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga
       61 atagcggaac acatagccgg tgctatactt agtctcgtta attactggga cataacatca
      121 agaggatatg aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt
      181 tatacccgca acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc
      241 caggttatcc agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc
      301 ggcgctaact tcattgccgt acatgaaatg ctggatggct tccgcaccgc actgatcgat
      361 catctggata ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact
      421 caagttatca acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag
      481 gatcacctga aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg
      541 attggcgaag cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg
      601 gataaattcc tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga
      661 actttagtac ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccacc
      721 tcaacgcact tatttagtgc atccatctgc tatctccagc tgattaagta aattttttgt
      781 atccacatca tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa
      841 taatcactgt gttgagtgca caattttagc gcaccagatt ggtgccccag aatggtgcat
      901 cttcagggta ttgccctata aatcgtgcat cacgtttttg ccgcatctcg aaaaatcaag
      961 gagttgcaaa actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg
     1021 tggatagaaa aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac
     1081 cctggctttt gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac
     1141 cgccttcgtt ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct
     1201 gtgggctgcc atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag
     1261 tgggatcatt ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat
     1321 caccgacgag cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt
     1381 agtgatggtg aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt
     1441 ggttgctgtt aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa
     1501 agatctgcgt cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc
     1561 agacgccgtt ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg
     1621 tcagttcaaa gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa
     1681 aggtagcgac gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg
     1741 aacttacaac gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac
     1801 ctgtaaagcg cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc
     1861 aaacccgctc cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga
     1921 acaacatatg cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg
     1981 tgccaaaatg accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt
     2041 gctggcaggt tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt
     2101 tattgaggtg atccgcggca cacctatcgt cgtccaggtg atgtttattt atttcgccct
     2161 gccgatggcg tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat
     2221 gatcaactcc ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa
     2281 aggttttcgt gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt
     2341 cattttaccg ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag
     2401 cattaaagac acctcgctgt ttattgtgat cggcgtggcg gaactgaccc gtcaggggca
     2461 agaaattatt gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta
     2521 tctgattatt accctggtgc taagctttat tttgcgtcgt ctggaaagaa ggatgaaaat
     2581 cctgtgattg aatttaaaaa cgtctccaag cactttggcc caacccaggt gcttcacaat
     2641 atcgatttga acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt
     2701 aaatcgaccc tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt
     2761 gtcgatggcc tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca
     2821 ggtatggtgt tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg
     2881 tttggtccgc tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag
     2941 ctgctggcga aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt
     3001 ggtcaacagc agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg
     3061 tttgatgaac cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg
     3121 caggatctgg ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc
     3181 gagaaagtag cttcgcggct gatctttatc gacaaaggcc ggattgcgga agatggcgat
     3241 ccgcaggtat tgatcaagaa cccgccgagc cagcgcttgc aggaattttt gcagcacgtc
     3301 tcttaacttc tctgcctccc tttcacccga aagggaggca caccagattc ctctcattta
     3361 aaatcgcccc tcccccagca tctatactta tctttttgct ctgttttctc actggaggag
     3421 tcatgcggtg gatcctgttc atcctcttct gcctgctggg cgcacctgcc cacgcggtat
     3481 ccatacccgg cgttacaacc acaacgacaa cggactcaac gactgaaccg gccccggaac
     3541 cggatatcga acaaaaaaaa gcggcctatg gcgcactggc ggatgtgctg gataacgaca
     3601 cctcgcgtaa agaattgatc gaccagttgc gtactgtcgc cgcgacgccc cctgcggaac
     3661 cggtcccgaa gatcgtgccg ccgacgctgg ttgaagagca aaccgtgctg caaaaggtca
     3721 ccgaagtcag ccgccattat ggtgaagccc tttccgcccg cttcgggcaa ctttatcgca
     3781 atatcaccgg ctccccgcat aagccattta atccacaaac cttcagcaat gcgctgagcc
     3841 atttttcaat gttagcggta ttagtgtttg gtttttactg gctgattcgc ctgtgcgcac
     3901 tgccgctgta tcgcaaaatg ggtcagtggg cgaggcaaaa aaatcgtgag cgcagcaact
     3961 ggttgcagct tccggcgatg attatcgggg cgtttattat cgacctgctg ttactggccc
     4021 tgacattatt tgtcggccag gtattaagcg acaacctgaa tgcaggcagt cgcaccatcg
     4081 ctttccaaca aagtttgttt ctcaacgcct ttgccctgat tgaatttttc aaagccgtac
     4141 tacgcctgat tttttgccca aacgtggcgg agctgcgccc gttcacgatt caggacgaga
     4201 gcgcccgtta ctggagtcgt cgcctgagct ggttaagcag tttgataggt tatggcctga
     4261 ttgtggccgt gccgattatc tctaatcagg tgaatgtaca gataggtgcg ctggcgaacg
     4321 tcatcattat gctgtgcatg accgtctggg cgttgtacct gatctttcgt aataaaaaag
     4381 agattaccca gcatttgctc aactttgcgg agcattcgct ggcctttttc agcctgttta
     4441 tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc gtgctgtttt
     4501 tcttttcgtt gttcgatccg ggcaacagcc tgaaatttat gatgggtgca acggtgcgca
     4561 gcctggcgat tattggtatc gcagcgtttg tttccggtat gttttcccgc tggctggcaa
     4621 aaaccatcac tctctcgcca catactcagc gtaactatcc agagttgcaa aaacggctga
     4681 atggctggct ttcggcggcg ctgaaaacgg cgcgtattct gaccgtctgc gtggcggtaa
     4741 tgttgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa aacggcgcgg
     4801 ggcagaaaac cgtagatatc ctgatccgta tcgcacttat tctttttttc tcggcggttg
     4861 gctggacggt gctcgccagt ttgatcgaaa accggctggc ttcggatatt catggccgcc
     4921 cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt ccgtaacgcg ctggcggtga
     4981 ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgtcaat atcgcgccat
     5041 tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa acgctggtga
     5101 aagatatcat taccggggtg tttattcagt ttgaaaacgg catgaacact ggagatttgg
     5161 tgactatcgg gccgttgacc ggcacagtgg aacggatgtc gattcgctcc gtgggcgtgc
     5221 gccaggatac cggggcgtat cacatcattc cgtggtcttc gataaccacc tttgctaact
     5281 tcgtccgcgg tattggttcg gtagtggcaa actacgatgt tgatcgccat gaagatgctg
     5341 ataaagccaa tcaggcactg aaagatgcgg tagcggaatt aatggaaaac gaagaaattc
     5401 gcgggctgat tattggtgaa ccgaattttg ccgggattgt cggcttaagc aataccgcgt
     5461 ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggta cgctttgccc
     5521 tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt tcgcgcgcca gtgcagactt
     5581 atcaggtgct gcctactccg ggcgcgaccc cggctgaacc gttaccgccg ggggagccaa
     5641 cgctttaacg ctggcgattg acaaaacggc ggcgctgctc gtcgttcata aaggtccagg
     5701 caataaagcg actctgcttt tgcccctggg ccatctcttt ttttaccacc ttcaccgcgc
     5761 ccacgtcggt cagagcacga tacaacggcg gtaagttttc accacgagat accagcgagg
     5821 taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt ttgataaagg
     5881 cgacttcacc accttcacac cacaactcct gttgctgacc accaaagttc agtgcatcgt
     5941 ctttgttcag ccccaggtta cggcgtttac gctcactacc tgcccgcgct gcagcggcgg
     6001 aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgttttta tggatgatgc
     6061 cgttaaaaat tgccccactc tctttttgcc gacgcagacg aatggcgcgg ttaagccccg
     6121 gattagcact gataatcgcc tgcgcactgc ttaacgcctg gctgctggtt tcactaccgg
     6181 taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc gcaccaacgc
     6241 cgatatccag aatgctggca ttcgctggaa ttgttccgct tgcttcagcc agtaaatcgg
     6301 caaggtgatg aatataatcc gcccggcccg gtactggtgg gcagagaaaa ccgtcgggga
     6361 tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc gccttcaccg
     6421 ccagcggatt ggcaaagtct acgctttgct ccccggcggg tgtaagcgtg aggaattgcc
     6481 tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga tgacggttgc
     6541 gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag cggcgtaaga
     6601 tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcattcccc aaaaccttaa
     6661 catggctatg gtttatggca attcatcata aaaatcagca ttatgcatgt cattaatttg
     6721 tcttattttg taaaaatata tcgttcaaaa aataagcaca tctctgtcaa ttttttgcgt
     6781 catttgcatc gcgcaggcga cctacactta tcaggcacta cccgttgtat ttatgaggat
     6841 gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg aatctctctc
     6901 acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg gcccgccggt
     6961 accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact ggcagaaaaa
     7021 gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa tgaccagatg
     7081 ttcggtactg caaccatcta caaataaccc caccaacgta actgcattcg cctctggatg
     7141 cagttttcgc ctgttttgta atcaaaaaac atcaaatatg aactcaatgt aaataaatgt
     7201 atttcttttt cgcgcaatgg gtgatagaaa atcgcgccaa gtgataatgc ttatcaaaat
     7261 tattatcact tgcacgaaca ctatcacggg attaacagtg gcatcgcatc cgcagagatg
     7321 ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag tcacctgcaa aatggaaaac
     7381 aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg tctttgtatt
     7441 ggcatcacgc ctgtggctca ggcactcgcc gccgaagggc aaactaacgc ggatgacacg
     7501 ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc cgatccgaaa
     7561 ttctcgcgtc cggtagcgga tactacccgc acgatgacgg tgatttctga acaagtgatt
     7621 aaagatcagg gcgcaaccaa ccttaccgac gcgctcaaaa acgcccccgg cg
//
LOCUS       DAZRVY010000172         7611 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZRVY010000172 DAZRVY010000000
VERSION     DAZRVY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.172"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHXOX_003824"
     CDS             171..701
                     /locus_tag="ACHXOX_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR7007187.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHXOX_003825"
     CDS             837..1502
                     /locus_tag="ACHXOX_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007188.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHXOX_003826"
     CDS             1824..2447
                     /locus_tag="ACHXOX_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR7007189.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHXOX_003827"
     CDS             2532..2750
                     /locus_tag="ACHXOX_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR7007190.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHXOX_003828"
     CDS             2728..3798
                     /locus_tag="ACHXOX_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7007191.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHXOX_003829"
     CDS             3933..5216
                     /locus_tag="ACHXOX_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR7007192.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHXOX_003830"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHXOX_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR7007193.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZRVY010000173         7600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZRVY010000173 DAZRVY010000000
VERSION     DAZRVY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.173"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(113..489)
                     /gene="rnpB"
                     /locus_tag="ACHXOX_003831"
     ncRNA           complement(113..489)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHXOX_003831"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(522..1667)
                     /gene="garK"
                     /locus_tag="ACHXOX_003832"
     CDS             complement(522..1667)
                     /gene="garK"
                     /locus_tag="ACHXOX_003832"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR7007194.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1764..2654)
                     /gene="garR"
                     /locus_tag="ACHXOX_003833"
     CDS             complement(1764..2654)
                     /gene="garR"
                     /locus_tag="ACHXOX_003833"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR7007195.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2684..3454)
                     /gene="garL"
                     /locus_tag="ACHXOX_003834"
     CDS             complement(2684..3454)
                     /gene="garL"
                     /locus_tag="ACHXOX_003834"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR7007196.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3470..4804)
                     /gene="garP"
                     /locus_tag="ACHXOX_003835"
     CDS             complement(3470..4804)
                     /gene="garP"
                     /locus_tag="ACHXOX_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR7007197.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5179..6750
                     /gene="garD"
                     /locus_tag="ACHXOX_003836"
     CDS             5179..6750
                     /gene="garD"
                     /locus_tag="ACHXOX_003836"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR7007198.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6899..7269
                     /locus_tag="ACHXOX_003837"
                     /pseudo
     CDS             6899..7269
                     /locus_tag="ACHXOX_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            7269..>7600
                     /gene="yhaV"
                     /locus_tag="ACHXOX_003838"
     CDS             7269..>7600
                     /gene="yhaV"
                     /locus_tag="ACHXOX_003838"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR7007199.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         1907 a         2037 c         1860 g         1796 t
ORIGIN      
        1 ggtgaaactg accgataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc
       61 tttccgctcc tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa tcaggtgaaa
      121 ctgaccgata agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac
      181 tggctcaagc agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc
      241 ttgctccggg tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac
      301 cgcacccttt cacccttacc tgatcccgct tgcgcgggcc atcggcggtt tgctctctgt
      361 tgcactggtc gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg
      421 actttcctcc cctccgcccg tctcccccga agaggacgac gacgaagcgg cgactgtctg
      481 gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt gtcaccccgc gttgcgcatt
      541 ccaatcgcca gtgtcgcggc gatattacgt gaagcacggc agatattgtc ataagccccg
      601 cggaatgctt cgtccaacgt acctatgctg gtcaatacgc tgaagaccgc atcgatgcca
      661 tgctgatgta caacgccaac atcatcggtc aggctacccg caatgccaat caccggttta
      721 tggtacttct tcgccacgtt tgcaacgcca atcggtacct tgccgtgaat actctggctg
      781 tcaatacgcc cttcaccggt gatcaccagc gtacaatcgt gaatatgttc ttccagattc
      841 agcgccgtag tgacgatttc aataccactt ttcagttccg caccaagaaa cgccatcagc
      901 gccgcgccca taccgcccgc agctcctgcg ccggggacat ctttcacatc aacatgcagc
      961 gcttttttaa tgacatcggc atagtgagag aggttattgt ccagctcaac aatcatcgct
     1021 tcactggctc ccttttgtgg gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga
     1081 ttggtgacat cacaagcgac gcgaatgacg caatctttta agcgcggatc gaggccggaa
     1141 atatcaatat cattcagagt attaagacta ccgccgccaa aaccaatttc attgccgttg
     1201 gcgtcgcata atttcgcccc cagcgcctgt accatgcctg cgccgccatc atttgtagcg
     1261 ctgccgccaa tgccgataat aatgtttgtt gcactgctct ccagcgcctg caggattaac
     1321 tcgcctgtgc cgcgtgaagt ggttacgagt ggatcgcgtt tttccgcagg taccagctcc
     1381 agcccactgg ccgccgccat ttcaataaac gcggttttgc catcgccgga gatcccccaa
     1441 ctggcattca ctttctcgcc cagcggccct gtaacccagg cgtgacgttc agccccctgg
     1501 gtggctgcaa tcatcgcttc caccgttcct tcaccaccgt cggcaaccgg aacagaaaca
     1561 tactgtgcat caggaaaaat ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg
     1621 gcagataaac tttctttata agagtctggg gcgattacga ttttcatacc tatgcctgtt
     1681 accacatgac gccggagggc gtttctctta ttcggcctag attccaggcc cggattgcaa
     1741 tacaccatcc gggcacgacg tcattaacga gtaacttcga ctttcgccag tttttcgtag
     1801 tagcacgcca gggcgctatg atccgccgtt cctaaaccat ctgcacgcag tgcctgcatc
     1861 atctccataa ccgcagctgt gagcagcagt tgtgcgccga cgccgtgaga agtatccagc
     1921 gcattcgcca gatccttaat atgcagatca atgcggaagc ccggcttgaa gttgcggtcc
     1981 atcaccatcg gcgctttggc atccagcacg gtactgcccg ccagcccacc gcgaattgcc
     2041 tgataaacca ggtccgggtt aacgcccgct ttggttgcca gcgttaacgc ttctgacatc
     2101 gcagcaatat tcagcgccac aatgacctga tttgccagtt tggtgacgtt acctgcaccg
     2161 atttccccgg tatgcaccac ggaacccgcc atcgctttca tcaaatcata gtatttgtcg
     2221 aaaatagcct tgtcgccgcc caccatcact gacagcgtac cgtcgatggc tttcggttca
     2281 ccgccgctca ccggagcatc cagcatatca atgcctttcg ctttcagcgc ttcgctgatt
     2341 tcacggcttg ccagcggtgc gatagaactc atatcgatca ataccgtacc tggcttcgcg
     2401 ccttcaataa tgccattctc acccagcgcc acctctttca catgagggga gtttggcagc
     2461 atggttatga tgacgtcgca ctgttcagcg atcgctttag ccgtagacgc tgtttctgca
     2521 cctgcagcaa tcacgtcagc aatagcttct gggttacggt cagcaaccac cagcgagtaa
     2581 cctgctttca gaaggttttt actcattggt ttacccataa tccccaggcc aataaaacca
     2641 actttcatag tcatatcaac catctctctt gttgcggtgg tggttatttt ttaaaggtat
     2701 cagccagttt ctgagtggca gagcggaaga cgccaagatc gctgccgaca gccacaaacg
     2761 tcgcgcccca ttccagataa cgacgcgcat cggcttcgac cggcgcgaga ataccgctgg
     2821 gtttgccgtg cgcgctggca cggttaaaaa tatgctgaat tgctttttgt acatccggat
     2881 gcgaggcatt gccgagatgg cccaatgccg cagccagatc gctggggccg acgaagatac
     2941 cgtctacacc ttcggtagcg gcaatagcat cgacgttatc tacgccctgc tgactttcta
     3001 tctggaccag aatagtaatg ttcttgttcg actgagcgaa ataatccgcc acggtgccaa
     3061 acatattggc gcggtgagaa acggagacgc cgcgaatgcc ttccggcggg taacgagttg
     3121 atgccaccgc ctgctctgct tcctcttttg tttctacaaa aggaatcagg aagttataga
     3181 aaccgatatc cagaagacgc ttaataatta ccggctcgtt ggtcggcact cgcactactg
     3241 gcgcgctggc gctgcctttc agggccatta actgcggaat aaacgtggaa atatcgtttg
     3301 gcgcatgttc gccatccagc accagccagt caaacccagc caaaccaaga acttcagtgc
     3361 taatcgggtt agagagtgct gaccagcaac caatttgtac ctgtttcgca gccagtgcgg
     3421 ctttgaattt attcgggaaa acatcgttat tcatcgctta tacctttgct tatttctgca
     3481 attccatacg tttaatgtcg ccaactacga agaggtagca gaccatcgcc atcagcgctg
     3541 aacatcccac gaaaaccagt gctgcattga aggagtgcag ttcacttacc aggtagccaa
     3601 tcaccagtgg agtgacaatg gaggcaacat tgccaaagac gttaaagacg ccgccacaga
     3661 ggccaacaat ctctttcggc gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc
     3721 ctttgccaaa aaaagccagc gccatcagca tgaccaccag cgtggtgttg ttggtgtagt
     3781 tacataagat gatggtggaa accagcaaca ttcccagcac aatcggtagc ttacgtgcca
     3841 gggtcaggga taaaccgcgt ttgatcagat aatccgagaa gacacctccc agcacgccgc
     3901 ccgcaaaacc acacagtgcc ggaatcgagg cgaccagacc cactttcaga atcgacatgc
     3961 ctttttcctg caccagataa atcgggaacc aggtgaggaa gaaccaggtg atggtgttga
     4021 taaaatattg tccgaaaaat acgcccaaca tcatgcggtt agagagcaat tgcttgatgt
     4081 aatgcagttt gggaccgctt gctgccgcgc tgcccggctt tttgtggtcc atatcgacca
     4141 ccgcgccatt ttcagagata aacttcagct cttccgcaga catacgtggg tgatctgtcg
     4201 ggttatgaat caacttgatc cacagcgccg tcagcacaaa accaatcccc cccataacgg
     4261 tgaagacgtg ctcccagccc caagcgaaag tcagccagcc aagcagcggc gaaaagagcg
     4321 ccagcgagaa atattgcgcc gagttaaaga tggcggaggc agtaccacgt tctttcgtcg
     4381 ggaaccaggc ggcgacaata cgggcgttcg ccgggaatga tggcgcttcc gagaacccca
     4441 gcataaagcg cataaagaac atggagatcc ctgcccaggc cagcgggaac atatcaacaa
     4501 agccttgcag gaaggtgaac agcgaccaga aaaagaggct gtaggtgtaa acttttttcg
     4561 agccaaactt atcaagcaac cagccgccgg ggatttgcat cagcaagtag gcccagccaa
     4621 aagcagagaa gatgtaaccc atcgaaaccg cacttaactg caactctttt gccacttcgg
     4681 taccagcaat agacagcgtt gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa
     4741 tcagtaatat taaatagctg gtatgcacgc ctttcttttt tacgtcaacg gtgtccagaa
     4801 tcattttatt tacctcgggt acttatgctg atttttatta ttatggggaa ggtgttattt
     4861 atgagtttta tttatgccgt tacgacaatg aactcgggaa ttagtataag cagcgggaga
     4921 ataataatca ttgtgcaaat gctaatttaa tcagtactat ttaaatatta ttttgagcat
     4981 atgcacataa ggttgcgcgc taaagcacag atttgcgctt taccttaccg ggcggcactg
     5041 caatccctga aatgattgac attgatcaca tttctgcgtt taaactcctg acattcttat
     5101 ttcacccaat gaagtcattt atttttaaat gagaccagat cctcatttta ataacccctg
     5161 gctggagaat attgcacaat ggccaacatc gaaatcagac aagaaacgcc aactgcgttt
     5221 tatataaaag ttcacgacac agataatgtg gcaattattg ttaatgataa tggcctgaaa
     5281 gcaggaacgc gttttccgga tgggctggaa ttaattgaac atattcccca ggggcataaa
     5341 gtcgcattgc tggacattcc agctaatggt gaaattattc gttatggcga agtgattggt
     5401 tacgccgtgc gcgcaatccc acgcggaagc tggatcgacg aatcaatggt tgtactaccg
     5461 gaagcgccgc cgttacacac gctgccactg gcaaccaaag tcccggcccc cttaccgccg
     5521 ctggaaggat acacctttga gggctatcgc aatgccgatg gcagcgtggg caccaaaaac
     5581 ctgctcggca tcaccaccag cgtccactgt gtggcaggcg tggtggacta tgtagtaaaa
     5641 atcattgaac gcgatctgct accgaaatac ccgaacgtcg atggcgtggt ggggctgaat
     5701 catttgtacg gctgtggcgt ggcgattaac gcaccggcgg cagttgtgcc tattcgtacc
     5761 atccacaata tttcgctgaa ccctaacttt ggcggcgaag taatggtgat tggtctgggt
     5821 tgtgaaaagt tgcagcctga gcgcctgctg gtcggcacgg atgatgtgca agctattcca
     5881 gtagaaagcg ccagcattgt cagtttgcag gatgaaaagc atgtcggctt tcagtccatg
     5941 gtcgaggata ttttgcaggt cgccgaacgc catctacaaa aactgaatca acggcagcga
     6001 gaaacctgcc cggcttcaga actggtcgtt ggtgtgcagt gcggtggcag cgatgcgttt
     6061 tctggtgtaa cggcaaaccc ggcggttggc tatgcgtctg atctactggt gcgctgcggc
     6121 gcaacggtga tgttttcaga agtaacggaa gtgcgtgacg cgatccatct gctgacacca
     6181 cgcgcagtga acgaagaggt cggcaaacgg ctgctggagg agatggagtg gtacgataac
     6241 tatctcaata tgggaaaaac cgaccgcagc gccaaccctt cgccgggcaa caagaaaggc
     6301 ggtctggcaa atgtggtgga gaaggcactc ggctccattg ctaaatcggg taaaagtgca
     6361 attgttgaag tgctgtcgcc cggtcaacgc ccgactaaac gcggattaat ttacgccgcg
     6421 acgccagcca gcgattttgt ctgtggcacg caacaggtgg cttcgggtat caccgtacaa
     6481 gtgtttacga ccggccgtgg tacgccgtac ggcctgatgg cggtacccgt aattaaaatg
     6541 gcaacccgca ccgagctggc gaaccgctgg tttgatttaa tggatattaa cgcgggcact
     6601 atcgctaccg gcgaagagag cattgaagag gtgggctgga agttgttcca ctttattctc
     6661 gacgtcgcca gcgggaagaa gaaaaccttc tcggatcaat gggggctgca taaccagctg
     6721 gcggtgttta acccggcacc ggtgacctga tttcttttgg aacgtccctc gcaaaacatg
     6781 gccttagtgc catgttttta ttgtttaaag cccccacgtc cattaataat gcatttgcat
     6841 taccttagtt caagcttata atttgagcag aaaacaggct gtaaaaggac aatgaatcat
     6901 gcccgctaat gctcgctctc acgctgtact gaccactgaa tcaaaggtca cgatacgcgg
     6961 acaaacaact atccccgcgc cagtgcgtga ggccttaaaa ctgaagccag gccaggacag
     7021 cattcattac gaaattctgc ctggtgggca agtatttatg tgccgactgg gagatgaaca
     7081 ggaggatcat actatgaatg catttttgcg ttttctggat acagatatcc agaacaaccc
     7141 gcaaaaaact cgtccattca acattcaaca ttcaacattc aacattcaac attcaacatt
     7201 caacaaggaa agaaacttgt cgctggcatg gacgtcaaca ttgatgatga gattggcgac
     7261 gacgaataat ggattttcca caaagggtta atggttgggc gctatatgct catccctgtt
     7321 ttcaggaaac ctacgacgct ttagttgccg aagtcgagac attaaaggga aaagatcctg
     7381 aaaattatca gagaaaagcc gccacaaagt tattggcggt agtccataaa gtgattgagg
     7441 agcatatcac ggtcaatcca tcatcaccgg cattccgtca tggcaagtcg ttaggctctg
     7501 ggaaaaataa agactggtca cgggtaaaat ttggtgcagg tcgttatcgt ctcttctttc
     7561 gttatagtga aaaagagaaa gtcatcattc tgggatggat
//
LOCUS       DAZRVY010000174         7436 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZRVY010000174 DAZRVY010000000
VERSION     DAZRVY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.174"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            105..365
                     /gene="dinJ"
                     /locus_tag="ACHXOX_003839"
     CDS             105..365
                     /gene="dinJ"
                     /locus_tag="ACHXOX_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR7007200.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            368..646
                     /gene="yafQ"
                     /locus_tag="ACHXOX_003840"
     CDS             368..646
                     /gene="yafQ"
                     /locus_tag="ACHXOX_003840"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR7007201.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            802..1542
                     /gene="dpaA"
                     /locus_tag="ACHXOX_003841"
     CDS             802..1542
                     /gene="dpaA"
                     /locus_tag="ACHXOX_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR7007202.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1513..2280)
                     /locus_tag="ACHXOX_003842"
     CDS             complement(1513..2280)
                     /locus_tag="ACHXOX_003842"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR7007203.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2486..3064)
                     /gene="lpcA"
                     /locus_tag="ACHXOX_003843"
     CDS             complement(2486..3064)
                     /gene="lpcA"
                     /locus_tag="ACHXOX_003843"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR7007204.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3304..5748
                     /gene="fadE"
                     /locus_tag="ACHXOX_003844"
     CDS             3304..5748
                     /gene="fadE"
                     /locus_tag="ACHXOX_003844"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR7007205.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5791..6237)
                     /gene="ivy"
                     /locus_tag="ACHXOX_003845"
     CDS             complement(5791..6237)
                     /gene="ivy"
                     /locus_tag="ACHXOX_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR7007206.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6418..7188
                     /gene="yafV"
                     /locus_tag="ACHXOX_003846"
     CDS             6418..7188
                     /gene="yafV"
                     /locus_tag="ACHXOX_003846"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR7007207.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(7230..>7436)
                     /locus_tag="ACHXOX_003847"
     CDS             complement(7230..>7436)
                     /locus_tag="ACHXOX_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7007208.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1766 a         1873 c         1972 g         1825 t
ORIGIN      
        1 attatgtaaa aacatccgtt tattaaatta ctcatggatt gtcgctgttg ctcatttgag
       61 ctacaattca agctgaataa atatacagca caggagatcc cccaatggct gctaacgcgt
      121 ttgttcgcgc ccgaatcgat gaagatctga agaatcaggc ggcggacgta ctggccggga
      181 tggggctgac catctctgac ctggttcgca taaccctcac aaaggtcgcg cgtgaaaagg
      241 cattgccgtt tgatttacgc gagcctaatc aattaaccat tcaatcaatc aaaaacagcg
      301 aagctggcgt tgatgttcat aaggccaaag acgctgatga tttatttgat aaattaggaa
      361 tttaaatatg attcaaaggg atattgaata ctcgggacaa ttttcaaagg atgtaaaact
      421 tgcacaaaag cgtcataagg atatgaataa attgaaatat cttatgacgc ttcttatcaa
      481 taatgcttta ccgcttccag ctgtttataa agaccacccg ctgcaaggtt catggaaagg
      541 ttatcgcgat gctcatgtcg aaccggactg gatcctgatt tacaaactta ccgataaact
      601 tttacgattt gagagaactg gaactcacgc ggcgctcttt gggtaaagca cgctgccgat
      661 gaaatttgta aaccctgcgc cgacaggctt gcgtaaaact cttttggtga taatgtgagc
      721 cttccaaatc cagactattc cgatttggga tctattttgc cgtcctggcg tgtgtaaccg
      781 ttttatcaag gaataagcag tatgcgtaaa atcgcattaa ttcttgcgat gttgttgatc
      841 ccgtgcgttt cgtttgccgg tttgctgggc agcagtagct cgaccacgcc agtgagcaaa
      901 gagtataagc agcagttgat gggatcccct gtctacatcc agatcttcaa ggaagaacgt
      961 acgctcgatc tctacgtcaa aatgggcgag caatatcagc tgctcgacag ttataaaatc
     1021 tgtaaatatt ccggcggctt agggccaaaa cagcgtcagg gcgatttcaa aagcccggaa
     1081 gggttttata gcgtccagcg taatcagtta aaaccagaca gccgttacta caaagcgatt
     1141 aatattggtt tccccaatgc ctatgaccgt gcgcatggtt atgaagggaa atacctgatg
     1201 atccacggcg attgtgtttc catcggctgc tacgcaatga ccaatcaggg tattgatgag
     1261 atcttccagt tcgttactgg tgcgctggtg tttggtcagc cgagcgtgca agtgagtatt
     1321 tacccgttcc gcatgaccga cgccaatatg aagcgccata aatattccaa ctttaaggac
     1381 ttctgggagc aactgaagcc gggttacgac tactttgagc aaacccgtaa gccaccgacc
     1441 gtttctgtgg tcaatggtcg ttacgtggtc agcaagccgt taagccacga agttgtgcag
     1501 ccacaactgg catcaaacta cacgctcccc gaggcaaaat aagcgccatt cgcctggcat
     1561 aatcttttgc caggtttcat tgcccgtcag cggctgtgtt gcaatcaccg tgaccacatc
     1621 attcggtgtg gtctgcgagc tgaagtcgat ttccacatcc tgatccagca gcgttgccac
     1681 gccaaacggc gcgcggcggg tgatccagtg taaattagtc gagcaatacg ccatcacata
     1741 gcgcccgtca gaaagcagca tgttgaaaac gcccttctgc cgcagttcat ccgccagtga
     1801 ggcgatatat ttaaacactg ccgccatgtt gcccggtgtg cgcgggtaac gctgcgttaa
     1861 tttatgcagg agccagcaga aggctttttc gctatcggtt tcaccgaccg ggcggaagtt
     1921 gccggtttcc agtgatttgt agcccgtcag ttgtccgtta tgggcataag tccagttacg
     1981 cccccataac tcacgggtaa acgggtgagt attttccagc gccacctcgc cccgattggc
     2041 ctggcgaata tgagccacca ccgaacagga ttttatcggg tagtcctgga caagtttggc
     2101 gatgggggaa ttaaagctgg gttgtggatc tttaaatgtg cgacagcctt taccttcgta
     2161 aaaggtaatg ccccagccat ctttatgtgg cccggttcca ccaccacgct gcaccagacc
     2221 ggtgaaacta aagcagatat cggtagggac gttggcgctc atcccgagca attcgcacat
     2281 gggtaacctc cacaacagcg ggagcgcatg gcccccggcg aagattgatt taagaatttt
     2341 caggccggat gcggcgtaaa cgtcttattc ggcctacggt tcgcgtacag atttgtaggc
     2401 atgataagac gcatcagcgt cgcatcaggc atcagcgcac aagtgccgga tgcggcgtaa
     2461 acgccttatc cggcctacgc cagacttact taaccatctc tttttcaatt aactggatca
     2521 ggatatggat cactttaatg tgaatctcct gaatgcggtc ggcataacca aagtgcggaa
     2581 cgcgaatttc gatatccgcc gtgccagcca ttttaccgcc gtctttaccg gtcagggtga
     2641 tcactttcat ccccttctca cgcgccgctg cgatcgcttt gatcacgttt gcagagttac
     2701 cggaggtgga gatccccagc agtacatcgc cttcgcgacc taccgcttca acgtagcggg
     2761 agaaaatatc attgaaaccg aaatcattac cgacgcagga aatatgacta acgtcagaaa
     2821 tagcaatcgc cgggtagccc ggacggtttt cacggtagcg accggtcaac tcttcggcaa
     2881 agtgcatagc gtcgcaatgg gaaccgccgt tgccgcagga aagcactttg ccaccggctt
     2941 taaagctgtc tgctaacagg accgccgcgc gctgaatggc gtgaatattg gcgtcatctt
     3001 ttaaaaagtt agccagcgtt tccgccgctt cgttcagttc gttacgaata agatcctggt
     3061 acatgaggat atccttcagc ataaatgtaa tagacaaaat gcagtgtacc ggataccgcc
     3121 aaaagcgaga agtacgggca ggtgctatga ccaggacttt ttgacctgaa gtgcggataa
     3181 aaacagcaac aatgtgagct ttgttgtaat tatattgtaa acacattgct aaatgttttt
     3241 acatccacta caaccatatc atcacaagtg gtcagacctc ctacaagtaa ggggcttttc
     3301 gttatgatga ttttgagtat tctcgctacg gttgtcctgc tcggcgcgtt gttctatcac
     3361 cgcgtgagct tatttatcag cagtctgatt ttgctcgcct ggacagccgc cctcggcgtt
     3421 gctggtctgt ggtcagcgtg ggtactggtg cctctggcca ttatcctcgt gccatttaac
     3481 tttgcgccta tgcgtaagtc gatgatttcc gcgccggtat ttcgcggttt ccgtaaggtg
     3541 atgccgccga tgtcgcgcac tgagaaagaa gcgattgatg cgggcaccac ctggtgggag
     3601 ggcgacttgt tccagggcaa gccggactgg aaaaagctgc ataactatcc gcagccgcgc
     3661 ctgaccgccg aagagcaagc gtttctcgac ggcccggtag aagaagcctg ccggatggcg
     3721 aatgatttcc agattactca tgagctggcg gatctgccgc cggagttgtg ggcgtacctt
     3781 aaagagcatc gcttcttcgc gatgattatc aaaaaagagt acggcggact ggagttctca
     3841 gcttatgccc agtctcgcgt gctgcaaaaa ctctccggcg tgagcgggat cctggcgatt
     3901 accgtcggcg tgccgaactc attaggcccg ggcgagctgc tgcaacatta cggcactgac
     3961 gagcagaaag atcactatct gccgcgtctg gcgcgtggtc aggagatccc ttgctttgca
     4021 ctgacaagcc cggaagcggg ttccgatgcg ggcgcgattc cggacaccgg gattgtctgc
     4081 atgggcgaat ggcagggcca gcaggtgctg gggatgcgtc tgacctggaa caaacgctat
     4141 attacgctgg caccgattgc gactgtgctt gggctggcgt ttaaactctc cgacccagaa
     4201 aagctgctgg gtggcgcaga agatttaggt attacctgtg cgctgatccc gaccaccacg
     4261 ccgggcgtgg aaattggtcg tcgccacttc ccgctgaacg taccgttcca gaacggaccg
     4321 acgcgcggta aagatgtctt cgtaccgatc gattacatca tcggtgggcc gaaaatggcc
     4381 gggcaaggct ggcggatgct ggtggagtgc ctctcggtag gccgcggcat caccctgcct
     4441 tccaactcaa ccggcggcgt gaaatcggta gcgctggcaa cgggcgcgta tgctcacatt
     4501 cgccgtcagt tcaaaatctc tatcggtaag atggaaggga ttgaagagcc gctggcgcgt
     4561 attgccggta atgcctacgt gatggatgct gcggcatcgc tgattaccta cggcattatg
     4621 ctcggcgaaa aacctgccgt gctgtcggct atcgttaagt atcactgtac ccaccgcggg
     4681 cagcagtcga ttattgatgc gatggatatc actggtggca aaggcattat gctcgggcaa
     4741 agcaacttcc tggcgcgtgc ttaccagggc gcaccgattg ccatcaccgt tgaaggggct
     4801 aacattctga cccgcagcat gatgatcttc ggacaaggag cgattcgttg ccatccgtac
     4861 gtgctggaag agatggaagc ggcgaagaac aatgacgtca acgcgttcga taaactgttg
     4921 ttcaaacata tcggtcacgt cggtagcaac aaagttcgca gcttctggct gggcctgacg
     4981 cgcggtttaa ccagcagcac gccaaccggc gatgccacta aacgctacta tcagcacctg
     5041 aaccgcctga gcgccaacct cgccctgctt tctgatgtct cgatggcagt gctggacggc
     5101 agcctgaaac gtcgcgagcg catctcggcc cgtctggggg atattttaag ccagctctac
     5161 ctcgcctctg ccgtgctgaa gcgttatgac gacgaaggcc gtaatgaagc cgacctgccg
     5221 ctggtgcact ggggcgtaca agatgcgctg tatcaggctg aacaggcgat ggatgattta
     5281 ctgcaaaact tcccgaaccg cgtggttgcc gggctgctga atgtggtgat cttcccgacc
     5341 ggacgtcatt atctggcacc ttctgacaag ctggatcata aagtggcgaa gattttacaa
     5401 gtgccgaacg ccacccgttc ccgcattggt cgcggtcagt acctgacgcc gagcgagcat
     5461 aatccggttg gcttgctgga agaggcgctg gtggatgtga ttgccgccga cccaattcat
     5521 cagcggatct gtaaagagct gggtaaaaac ctgccgttta cccgtctgga tgaactggcg
     5581 cacaacgcgc tggcgaaggg gctgattgat aaagatgaag ccgctattct ggtgaaagct
     5641 gaagaaagcc gtctgcgcag tattaacgtt gatgactttg atccggaaga gctggcgacg
     5701 aagccggtaa agttgccgga gaaagtgcgg aaagttgaag ccgcgtaatt gaataacgga
     5761 gccgaaaggc tccgtttctt tatccgctaa ttatttaaaa ttaaagccat ccggatggtt
     5821 ttccaggctg ccggtcaacg ccgcgaacag caccgtttta ccatcaatcg aaagcgcatc
     5881 gttcacattc agccaggtga gtttctcttg cgacgttttc tcatcaatag tcgagaacag
     5941 ccccgtcatc tgattagatt tctcggacca catcacagcg atacgttgcg agccacagtc
     6001 atgcggtttg cacgcgctca tcacctgata cgtatcatct cccaacgtta cggtttgtgc
     6061 gggagtataa gtaccgcctt tcatcaccca ggcaggcagc ttatgccctt gtaccatctg
     6121 attaaatgca gctttggtgg tttcgccctt tgcaaggctg ctaatggtta aatcatcctg
     6181 cgccattgca ctggtggcga tgaccagagc ggcgactgtc gttattgtct taaacatcat
     6241 tcctcccgag cttatcctgc ccatgttatt aaccttctaa agcctggcga taacacgata
     6301 atttgcaaat cagatatttc caatatcagc atttgttatc aatttattct gcgctaaggc
     6361 aactgcctga taaggtgtta aagttataaa acccttcgca taaaggagtt gtacaccgtg
     6421 cctggtttga agattacgct tttgcagcaa ccactggtgt ggatggatgg ccctgccaac
     6481 ctgcgtcatt ttgatcgtca actggaaggt attaccgggc gcgatgtgat cgttctaccg
     6541 aagatgttta ccagcggctt tgccatggaa gcggcagctt cgtcgctagc acaagatgac
     6601 gtagtggact ggatgacagc taaggcgcag cagtgcaatg cgctgattgc aggcagtgtt
     6661 gcattacaaa cggagtctgg ttcggttaac cgctttttgc tggttgagcc gggcggcacg
     6721 gtacattttt atgataagcg tcatctgttc cgcatggcag atgagcatct acattataaa
     6781 gcgggcaatg cgcgagtgat tgtggaatgg cgcggctggc gtattttgcc gttggtgtgc
     6841 tacgacttac gttttccggt gtggtcgcgc aatctcaacg attatgacct cgccatctac
     6901 gttgccaact ggcctgctcc gcgctctctg cactggcagg cattgctgac ggcccgcgcg
     6961 attgagaatc aggcgtatgt ggcgggatgc aatcgcgtcg gcagcgatgg caacggctgc
     7021 cattatcgcg gtgacagccg ggtgattaat ccacaaggcg agattatcgc tactgccgac
     7081 gcgcatcagg caacgcgcat tgatgcggag ctgtcgatga tggcattgcg ggaatataga
     7141 gaaaagtttc cggcatggca ggatgcggat gagtttaggt tgcggtgagt gggggttgtg
     7201 tttaaggctg ggggagagtc ggggtaagac tacgaaagcc cgctctccgc aaggactgac
     7261 gccagatagt ttctgtccat agctgctttt cgcatcttac gtcttaaccc tgccttgaat
     7321 accttatcat tcgtcaaaat attaatagcg atgtgccgta tccctgaaaa taattctgct
     7381 gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgcca
//
LOCUS       DAZRVY010000175         7298 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZRVY010000175 DAZRVY010000000
VERSION     DAZRVY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.175"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            121..1185
                     /gene="ulaG"
                     /locus_tag="ACHXOX_003848"
     CDS             121..1185
                     /gene="ulaG"
                     /locus_tag="ACHXOX_003848"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR7007209.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1293..2048
                     /gene="ulaR"
                     /locus_tag="ACHXOX_003849"
     CDS             1293..2048
                     /gene="ulaR"
                     /locus_tag="ACHXOX_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR7007210.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2045..2794)
                     /gene="yjfP"
                     /locus_tag="ACHXOX_003850"
     CDS             complement(2045..2794)
                     /gene="yjfP"
                     /locus_tag="ACHXOX_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7007211.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2976..3305
                     /gene="bsmA"
                     /locus_tag="ACHXOX_003851"
     CDS             2976..3305
                     /gene="bsmA"
                     /locus_tag="ACHXOX_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR7007212.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3454..3729
                     /gene="yjfN"
                     /locus_tag="ACHXOX_003852"
     CDS             3454..3729
                     /gene="yjfN"
                     /locus_tag="ACHXOX_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR7007213.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3846..5471)
                     /locus_tag="ACHXOX_003853"
     CDS             complement(3846..5471)
                     /locus_tag="ACHXOX_003853"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR7007214.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5555..6718)
                     /locus_tag="ACHXOX_003854"
     CDS             complement(5555..6718)
                     /locus_tag="ACHXOX_003854"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR7007215.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6721..>7298)
                     /locus_tag="ACHXOX_003855"
     CDS             complement(6721..>7298)
                     /locus_tag="ACHXOX_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR7007216.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1901 a         1949 c         1797 g         1651 t
ORIGIN      
        1 aacaacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc ttcctgaaaa aatagtgtca
       61 ggattaatca aaaccaatca catattgatt cgaattgaac tatcatgaag gtaaatggcg
      121 atgagtaaag tgaaaagtat cacccgagaa tcctggatcc tgagcacttt cccggagtgg
      181 ggtagctggc tgaatgaaga aattgaacaa gagcaggtcg cccctggcac atttgcgatg
      241 tggtggctag gctgcaccgg gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt
      301 gatttctggt gcggcactgg caaacaaagt cacggtaacc cgttaatgaa acagggtcac
      361 cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac caccccgttt
      421 gttcttgatc cgtttgcgat tcgccagatc gacgcggtac tggcgactca cgatcacaac
      481 gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga actgtgctga tgacgtacca
      541 ttcattggac cgaaaacctg cgttgatttg tggattggct ggggcgtacc gaaagagcgt
      601 tgcatcgtgg tcaaaccggg cgatgttgta aaagtgaaag acattgaaat tcatgcgctt
      661 gacgctttcg accgtactgc actgatcacc ctgcctgccg atcaaaaagc ggctggcgta
      721 ctgccagatg gcatggacga tcgcgcagta aactacctgt tcaaaacccc tggcggttcc
      781 ctatatcaca gtggcgattc ccactactct aactattatg cgaagcacgg taacgaacat
      841 cagatcgatg tggcgttagg atcgtatggt gagaacccgc gcggtattac cgacaaaatg
      901 accagcgccg atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc
      961 caccacgaca tctggtcaaa cttccaggcc gatccgcaag agatccgcgt gctgtgggag
     1021 atgaaaaaag atcgtctgaa gtatggcttc aagccgttta tctggcaggt tggcggcaaa
     1081 tttacctggc cgctggataa agacaacttc gagtaccact atccgcgcgg tttcgatgat
     1141 tgcttcacta ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt cgtttatacc
     1201 ggatagcgtg gagaagttcg cgctatccgg cgtagtattt cctagctatt tcaaatatca
     1261 tctaaaaaaa tcaaatttta tcggaatagc tcatgactga agcacaaaga catcaaatcc
     1321 tcctggaaat gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg
     1381 gaatttcgcc tgccactgcg cgacgcgata tcaataaact tgacgaacgc ggcaaactga
     1441 aaaaggtgcg caatggcgca gaagctatta cccaacagcg cccgcgctgg acgccgatga
     1501 atctgcatca ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc
     1561 tggttaatcc gggcgaaagc gtagtcatca actgcggctc caccgcgttt ctgcttgggc
     1621 gggaaatgtg tggcaagcca gtgcaaatca tcactaatta tctaccgctg gcaaattacc
     1681 tgatcgatca agaacatgac agcgtgatca ttatgggtgg acagtacaac aaaagtcagt
     1741 ccatcacttt aagcccgcag ggcagcgaaa acagtctcta tgccgggcac tggatgttta
     1801 ccagcggaaa agggctgacc gcagaagggt tgtataaaac cgatatgctg acagcaatgg
     1861 cagagcagaa gatgctgagc gtggtaggga aactggtggt actggttgat agcagtaaga
     1921 ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca aatcgatatg cttatcaccg
     1981 gcaaaaatgc taacccggaa atcctgcaac aactggaagc gcaaggggtc agcattctgc
     2041 gtgtttaaag atgctggcgg aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc
     2101 ggtgacgcac gcctggctgc catgaacagg ttagcagttt atcccgtccc gtctcgctta
     2161 aggcctgctg caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc
     2221 acagcagtag aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca
     2281 gcggcgcgat aatgttattg aattcattct gctgtgctgc cgtttcaggt atcagcggtg
     2341 gaaacagtga acgggcgagc gatgtaaaat agcccgatcc catcatgctg gcggtacatt
     2401 tcaccgtggg gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc gacgcgccac
     2461 cgactgccag acggtcatca agcagccagt tttcttcggc tattgccgca cgtaaagtag
     2521 tgaattcctg catactttgt agcaagattt gccagaattg atttaaccgc cgcgcttcgt
     2581 cgccactaaa acggctaccg tgatcgggcg catccggcat gatcacccgc aaaccagcct
     2641 gcgccagcgc aacggcaaaa tagctataca ccaggctgga tgaagtaaag ccgtgataaa
     2701 aaattacgca cggtaacggg gtatcttttt gcccgacagg ataagcatga agaacgggaa
     2761 tatctgccag ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg tctggtatga
     2821 catgaagaaa tccttagcac aagtttctgc tggcggtatt agttcataat gttgagatgt
     2881 gggttacgct ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca attcgggaac
     2941 attcccccaa agttaaatta ataaggcact acactatggt tagcaggaaa cgtaatagcg
     3001 ttatttaccg gtttgccagt ttattattgg tgttgatgtt aagtgcctgt agcgcactgc
     3061 aaggtacgcc acagccagca ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc
     3121 agacgcaagg gttacagcga ataggtagcg taagcacgat ggttcggggt tctccggatg
     3181 acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa agctgattat tacgttgtcg
     3241 tcatggtgga cgaaaccatc gtgacaggac agtggtattc acaagccatt ttgtaccgta
     3301 aataaatcag acaacttaca tagtcagctt tacattgctt tgcgtcccta tgcgtttacc
     3361 tgtgcacaat aaattaccgg ctggaagcct gggcgtttat taccgcgatg gaatgccctg
     3421 caacatgtgg ggaaacgaaa aatggagctg acgatgaaac aattacttgc ctcaccctcg
     3481 ctgcaattag tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct
     3541 gactgcgtga cgggcctgaa tgaaataggt cagatctcag ttagcaatat ttcaggggat
     3601 ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac
     3661 cgcattatta ccatgtacga agaccagcag cctgataact ggcgcgtaca ggcgatactg
     3721 tatgcatgag tgtcatctac agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc
     3781 cggcctacaa ttttcacata tttcattgag ttaatcgaaa ccagcgtcgc atcagtcgta
     3841 tacgtttaca aacacactcc ccccgtcgcc cgcagcaata aatcattctg gatctgctct
     3901 gacaaccgta cgccgccgcg cgtatctaac atcacctgac accacgcctg cgccattggc
     3961 ggagaagcat atttcaacat ttgcgcaccg cagcccagca ggaatagctg atgagtaatc
     4021 tctcgcccca gttcttcagc tggcttacgc agctgctgct gtaaacgacg aaccgcgcga
     4081 tcaaaatagc gatcctgccc tttcacttcc acaaatgctt ccgacaataa gtcgtatacg
     4141 cccgcttgct tattgagaac gcgcaacaca tccaggcaca taatattgcc ggaaccttcc
     4201 caaatactgt ttaccggcat ctcccggtaa agccgcggca gctcgctctc ctcgcaataa
     4261 ccaatgccgc ccagcacctc catcgcttcg gccacaaacg gcatacctcg tttgcagatc
     4321 acaaattttg ccgcaggcgt aaataaacgc gcccacaggg cttctttggc atcggcgcgc
     4381 cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt gcccttcaag ctgaagtgcc
     4441 atgcgactta agacatgacg cataaggggc tgttggatca atggattacc aaaaacatgg
     4501 cgttgatgtg catgataaat cgccagcgaa aacgcacggc gcatcatggc atggctaccc
     4561 agggcgcaat caaaacgcgt catccccccc attttcagga tcagacgaat tccttcccct
     4621 tcctgcccca ataaccagcc aatggcatcc tgaaactcca cttcacaact ggcgttagag
     4681 cgattaccca gcttatcttt cagccgttcg aggcgaatcg cgttgcgttg cccgtcaggc
     4741 aaaaagcgcg gcacaaaaaa acaggacaga ccgcccgtgg tctgcgccag caccagatgc
     4801 gcatcgcttt gcggaaccga gaaaaaccat ttatgcccca ccagccgata agagccatct
     4861 tccaaacgct ctgcacgggt ggtgttgctc atgacatcgg aaccaccctg cttttccgtc
     4921 attcccatgc caatcaacaa accgcgtttt tgcccacctg gcaataagtg agaatcgtag
     4981 cgatcgctca gcagcggcgt ggtccagtcc tgaaacggcg tgggtaacat ctgtaacaac
     5041 aatggcgtgg cggcaaaggt catcgttatt ggacataacg acccagcctc aacctgcgca
     5101 tgtaacataa aacgcgccgc gcgcgccaca aatgcgccgg agcgagcgtc ttcttcccag
     5161 gcaagattgt gcacccgatt ggtacataac acctgcatca gcaggtgcca tgcgggatgg
     5221 aaacgaacat cgtccagacg ccgcccctgg gcatcgtagc gcaacagttc aggcgggttt
     5281 acattcgcca gccgccccag ttcaagggat tcagccgttc ctaactgctg accaatactg
     5341 gctagaaaat cgctatccca gccagcacct tcacgcgtta ccgcttcgca aagcgcgcca
     5401 tcagacaggt ataagttgct gttatttaat ggtataggtt gattaaaaac ggtgtgagtt
     5461 tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc attagtatgg ccactgccat
     5521 ggattcatga cagaaagaga ttgctaaaac attcttatcc agcaatgtaa tgcggaacga
     5581 aacgtgaggt gtctttggtg atcagtgtgt tatcttcacg gatccccatt ccgcacgctt
     5641 catcatcgac aatccagcta tcgatgagtg tgtagctatc gccaaatcgc ggcagaggtt
     5701 gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc atcagcgtgg tcaacgacgt
     5761 tattctgacc gtcgaaaatg gtgacgtttc cgccttcgcg cgagtagatc ggtttacgca
     5821 catagctttc gccagcggca atctgcgatt tttcaccctc aaaccaggat gcaagaaggt
     5881 taggatgacc agggaagaag cgccaaagca gcggcattag ccctttatta ctcaagatac
     5941 ttttccataa cggctccacc cattgctcgc gacgcttgca cagcagcgga ccgttatcgt
     6001 cacgcatcat ccactccagc ggatacagct taaatgcacg ctggatgaca ttatcatcaa
     6061 gatcggtcag tacaccgccg acgcccaaac cgagatcttc aatgtagata aaccgcgact
     6121 cctgccctgc ctgctgggcg cagtcctgca agtacagcac ggtagtcctg tcttcatcgg
     6181 tgtcctgaca gcagcagaaa taaaacggct cccggctata aagctcgcta aagcgtgaaa
     6241 tcaggcgttc ctgaatggca ttgtactgat cggcatcacg cggaataatg ccgctgcgcc
     6301 gggcatcctc cagccacaac cactggaaat aagccgactc atacaacgaa gtcggcgtat
     6361 cggcgttgta ctccagcagc ttcaccggcg cattgccaca ccaggcaaaa tccatccggc
     6421 catacagcga aggatcgctg gcgtgccagc tttcggcgat cacatcccag tacaacggcg
     6481 ggatcgccag ttgcgtcagg atctcttcgt ctttaaccgc gcgatccacc acctcaaggc
     6541 acatctgatg cagttccgca gttggttttt cgatctgctc ttcaatctgg cgcagagtaa
     6601 aacggtaagc ccgactctcg tcccaataga tttcattgtc gatgatatga aagtcgaaac
     6661 cgttgtcagc ggcaatttgg tccagatccc gccgcacagg aacgttgtgt ctcagcatga
     6721 ttagcctccc caatgcccac gggcgctgga agaacgacca tagccgccgc gcgaaacggt
     6781 tgacgctttt ttggtggtaa agccgcctga agagtaagac tcttttttgc cagaaccgga
     6841 gcgccaggag taatcaccgg aagtgttgcg ccagacgggg cgggaagcaa atgaggatcc
     6901 gccgctgtta tagacgaacg gctcatcgcg atctttacga ataacccggc ttaacaaaaa
     6961 tccagaaacg accgggatcc aactctgttc aacattgtcg tagtagcaat tttcgtactt
     7021 agactgacag ttctgctgag tcatattctt cggaacatcg gcataaaatg ccgttttggc
     7081 gttgttccag ccacgcgcgc aaatgtcggc attattaccg tcatcaatac aatcctgcac
     7141 tgtcgcgtaa aacgttccgt cgccgtcgtt atcgacatcg ctactatcgc cacaaccttt
     7201 caatacgaaa aaagcggcac cgcccattag cgccaacgtt aagtattttt gtgcgtagcg
     7261 attgcgacgc ggttcaaaag gattattcgg tctaccaa
//
LOCUS       DAZRVY010000176         6854 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZRVY010000176 DAZRVY010000000
VERSION     DAZRVY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.176"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /gene="cas6e"
                     /locus_tag="ACHXOX_003856"
     CDS             complement(<1..215)
                     /gene="cas6e"
                     /locus_tag="ACHXOX_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR7007217.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(140..886)
                     /gene="cas5e"
                     /locus_tag="ACHXOX_003857"
     CDS             complement(140..886)
                     /gene="cas5e"
                     /locus_tag="ACHXOX_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR7007218.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="ACHXOX_003858"
     CDS             complement(897..1952)
                     /gene="cas7e"
                     /locus_tag="ACHXOX_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR7007219.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1964..2500)
                     /gene="casB"
                     /locus_tag="ACHXOX_003859"
                     /gene_synonym="cse2"
     CDS             complement(1964..2500)
                     /gene="casB"
                     /locus_tag="ACHXOX_003859"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR7007220.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2497..4059)
                     /gene="casA"
                     /locus_tag="ACHXOX_003860"
                     /gene_synonym="cse1"
     CDS             complement(2497..4059)
                     /gene="casA"
                     /locus_tag="ACHXOX_003860"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR7007221.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4157..>6854)
                     /gene="cas3"
                     /locus_tag="ACHXOX_003861"
     CDS             complement(4157..>6854)
                     /gene="cas3"
                     /locus_tag="ACHXOX_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR7007222.1"
                     /translation="RKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDV
                     AAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPE
                     LKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTG
                     HHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWY
                     ISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGIN
                     TLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG
                     LFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVG
                     SEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKI
                     LLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQ
                     EAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQG
                     KCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKED
                     SSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDG
                     KDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMP
                     HAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYL
                     PEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLE
                     GEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1659 a         1863 c         1624 g         1708 t
ORIGIN      
        1 tggagttctt cccgacgata aagaaattgc ctttctttgc cgccagggaa aagatcccac
       61 agccactgat gcatcacata ttcaccgcga tccaccagat gcagcaactg cgcaggcgaa
      121 agctggccgg tatgtaaggt tattcttgag aggtacatgg ttcctccttg ctgagccacg
      181 gcccctgatt gacggtgcgt tccccaaaca gccactgctg acgatttaaa ggaacatccc
      241 ggcggcgtaa tattttgctc gcgaccaggc cgtcgtgttt cccttcccac cagcattcat
      301 cctgaagttt cgggagtgag actttcagtt tatggaaatg atcctgatac tgttggtatg
      361 cattacataa ggcatcacac gcgttgcctt cgagcagtaa cggcgcaaga ggtaatgcca
      421 gcggatgact ttttcgcccc agataaagcg gaaaaactgg atggcgtaaa ccgtcctgca
      481 actgttcgag gctgtaaggc gcatcggctg ttgttgccac cgccaccatc caccaggcat
      541 cggtgtagta gtcgcgccgg gagataatcg cgctcaaaag ctcagggtcg ctcaactctt
      601 cacgacggct gaaataacgt gctttacgca cctcttttgg catctggacc gtgtgataat
      661 cccgtgccca gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg cgattaaacg
      721 cgtttaagcg ttcggtatca tcacgccgaa tccctacccc ggcagccagt agccccagca
      781 atgctgagcg cgaaggaagt tcatgggtat gacgaacttc acctggagca tcgacacccc
      841 aggacgccat cggcccatga agctgaaaaa tcagatattg gctcataagc cgccccttac
      901 gcgcagataa agtccagcac gtccttcatg cttccctgtt tgttcatcac gtcaaaactt
      961 gcgcattcgg tcttctgttc gtagaccgta ttcatgtttt cgcgaagcgt tgtaatacgc
     1021 tgcaccgcca catctaactg acgtgtgcca ttaatgggtt catagaaagc cgccgccaga
     1081 gagcgtggtt gatcggtgcc tttttctgcc agcgcccagg aagcataggc acggctggca
     1141 aagctatttt gtttaccggt tggggaaact ttaagcgcgg cttccgtaaa agcgcgtatg
     1201 gtctgattag ctaacgcttc gtcaccgccg aggttttcta ccagcagatc tttatcaatg
     1261 cagatatagg tgtagaaaag tgctgaaccg aatccggttt ctccgagatg cccggcaccg
     1321 gcatcttcag aggcctggcg caaatcatca acggcggtga aaaaatcatc ttcgacaatc
     1381 gtttcactga caccaaacgc atgcgcgacc tggcaggcgg cttcaacatt aaactcaggt
     1441 ttattcgcca gcatacgacc aaacatagcg atatctaccg ctatacggtc tttacgtaac
     1501 aacgcgagat cttcctcttt tggcgcgcgc ttttcttctg ccagttgatg agcaagcgct
     1561 tttacggcgt caaattctgc cgggctgata tgaactaatt gttcagtttc ggcgttagtg
     1621 agcggatctt ttggtttttt gtcattttta gctttcccaa gataatcagc aatttttgcc
     1681 gcccattcga tggctttttt atcttcaatc cctttctcaa tcaggatagt tgccgcctca
     1741 cgcgcaatac gcccactgcg aataccaata tggcccgcca gtgcctgttc aaaaagtgca
     1801 gaagtgcgcc acgcacgttt cagactttgc gaggaaacgc gcagacgcgt tgctccaccc
     1861 agcaccacag tttttggcgc accggtgtca tcacggttaa ggttggcagc agggtaagcg
     1921 gttaacacat gaagctgaat aaatgttgtc atgagatagt cctttattga ttttgttcgt
     1981 tatcggcgtc gccagcctgg taatattcca gcgcccagcg gatacggata aattcggttg
     2041 gccgctgctg gcggcggtga tgattgagca ggtcgtcgct ctcctggcac cagcggaaaa
     2101 tgccatctgc cagggagtca agattgacgg aaccgtttaa taatctgacg gcgcggcgta
     2161 actggcgcag taattcatcc ggtgttttta ctgctgacag gcgagtaaaa cgcccctttg
     2221 acattaccgc cgccagttgc gcggcaaaag gctgccgttc atcaatggct ttaacattgg
     2281 cgctaactgc ggctatcagc gccagtgcgg tgatacgcca ctcaggttca tcctgccact
     2341 ttatttgtct gttcttcaga aacaggcgaa atccatccgt cagacaaaca tcattaaccg
     2401 tcgtactacg ccgcagactg gcacgttcgc cgcgtttctc ctgcaattcc tcgtgccatt
     2461 tgcgcagcgt ggctttgtgc tcctctttta caatactcat tcagcagcct cctgcttttt
     2521 ctccctggcg gctttagcac tttgcttctc cgccgatgtt gtaaaatatt tcttgcgcgc
     2581 ggtcatgatg cgttccaaat caacgggctc ataaggattg gtgaatacac gctcgtcaaa
     2641 atcctgacgc gcgaataacc aaatttcctt attccatttg ctgagtaatt catccgcatc
     2701 ctgaccttct tcaatttggc gcactaacct caggaagcga tgctgagttt tgttccagaa
     2761 gtcgatatcc acaaaactaa aatcacccct tgcacctttt ggatcggaga accatgcttc
     2821 tttcaatgca ctccgtaaca gacttagaat ccgtgaagcc gtttgcgcag ccagccgcag
     2881 tttcggtatc tggccttctt ttttattgag cagcagcggg aaatgatgtt cgtaccaaca
     2941 gcgcgctttc atgttgtcga aatcatagcc aaatccccac aggcccactt ttgcctgctt
     3001 gagactgctg gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga
     3061 cttacccgtt tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt
     3121 gaccgagtaa aactcgccgc cctctttaag tagtacacgg taaggcgtta agggatgctg
     3181 ccacatggca tagttagcgc cgtaattttt ggtcgtcatc aggctcagaa gcgcgtcgct
     3241 ctgctcaccg caaatatcgc agttgccgac tgtggtggta ttaaaatcaa tacgaatacg
     3301 gcgaggcatt ccccagtacg cctggagttt attgacctgc tcatcggtta ccaccgcacc
     3361 ggccagttcg ctgatgcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt
     3421 gggtagcggt aaatcggctt catcctgcgg catgacattg atccacagtt tgcgccacaa
     3481 gggagtttgt tgattaccct gatattcctg taattcaatc agagtggtca tcggcccacc
     3541 gccgcgtaaa ccggtgcggt agcctttgcc acctgacggc gcattcaact gtagggagaa
     3601 cagagctaac gcagagcaat gtgggcacag gtattccgtt acaccacgct tgataaagtg
     3661 gtctttatta aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgaccgg
     3721 aactttatct ccggtgagcg cctcgaaatc ctgtataaat gaaggggaat ctggcccaaa
     3781 ctggaaagca tgttctaatg acagcaatgc ttcccgtagc ttttcagctt ccagtccgtc
     3841 ttcccagata tcatcccaac gacgatgatc ttttggcgcg aagctggttt gtagtagccc
     3901 cagcaaaaac tgccataccg caccctggag atctgcccgc ggcgcagaaa tatcgacaac
     3961 attttcatcc gccagatcga ctggcgccag tttgcctgtc gttccgtctt taaaacgaac
     4021 gggcaaccac ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag
     4081 accatcccag gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag
     4141 tcctgtcgca gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccgc
     4201 aactttcgtc atcgttgact aaaataacgt ttgccatctc cggatcttgt cgctgttcaa
     4261 tgcaccacag cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc
     4321 accagcttcg gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag
     4381 gcttcaccac accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac
     4441 gtgtcgacag tttttctggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt
     4501 tcagcacaaa ctttttagcc attgcgcgat cacagtaata tttgcccact tgctcctgct
     4561 cgctgcgggc aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa
     4621 gccgggctgc gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc
     4681 agattcgccc atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt
     4741 cgtcgccagg agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct
     4801 cgtcctttcc gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc
     4861 ctgctcgctg aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac
     4921 aatcgacact ctgctctaat acctgagtac aaataaggac ttttccggca cgctgtgagc
     4981 tatcttcttt accaaagcgt gccagtgtct ccgtttcaat tcgctggcga tcgctaaaag
     5041 caaagcggct atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct
     5101 gacgatgaac cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct
     5161 gacttaccgc cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc
     5221 tgacgctacg ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg
     5281 tgacatgcgt cagccagggg taatcatcct tttccaggaa cggagcttct tgcaggccct
     5341 ctgtaccccg cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag
     5401 aaagcaaaat aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc
     5461 acgacatgta agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc
     5521 ccagaagccg cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg
     5581 tgccgacgcc aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac
     5641 atccctgact gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga
     5701 gtgattcatt aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc
     5761 gggattctgg cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg
     5821 ccgttgccat tgttggcaga ccaaaaaaca gcccctgagc cttccccgca gctatcaacc
     5881 tgtgcgccag gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa
     5941 ataactgcgg tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg
     6001 gattctcaat aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta
     6061 gaggaaagac ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa
     6121 tatccattgg gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga
     6181 gcacgacggt tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat
     6241 catcccagaa tgcggggatc tctatgagcg gaaacagtgg tttgatttca aggagaaaat
     6301 ctcgtgctgc agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg
     6361 gccgaccatg atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc
     6421 gcggagagag cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca
     6481 accagtagcc cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa
     6541 tcttgagttc agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt
     6601 gccagcacag caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt
     6661 gacgaaaata atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg
     6721 ctacatccag cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc
     6781 gtcgggtttt tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta
     6841 aaggatattt acgc
//
LOCUS       DAZRVY010000177         6825 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZRVY010000177 DAZRVY010000000
VERSION     DAZRVY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.177"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHXOX_003862"
     CDS             complement(155..667)
                     /locus_tag="ACHXOX_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR7007223.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHXOX_003863"
     CDS             862..1626
                     /locus_tag="ACHXOX_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007224.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHXOX_003864"
     CDS             1577..2980
                     /locus_tag="ACHXOX_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR7007225.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHXOX_003865"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHXOX_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHXOX_003866"
     CDS             4337..5080
                     /locus_tag="ACHXOX_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR7007226.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLKAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHXOX_003867"
     CDS             5084..6304
                     /locus_tag="ACHXOX_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR7007227.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHXOX_003868"
     CDS             6308..6814
                     /locus_tag="ACHXOX_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007228.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1867 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcaaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacga
//
LOCUS       DAZRVY010000178         6821 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZRVY010000178 DAZRVY010000000
VERSION     DAZRVY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.178"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(270..755)
                     /gene="sixA"
                     /locus_tag="ACHXOX_003869"
     CDS             complement(270..755)
                     /gene="sixA"
                     /locus_tag="ACHXOX_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR7007229.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(958..3102)
                     /gene="fadJ"
                     /locus_tag="ACHXOX_003870"
     CDS             complement(958..3102)
                     /gene="fadJ"
                     /locus_tag="ACHXOX_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR7007230.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3102..4412)
                     /gene="fadI"
                     /locus_tag="ACHXOX_003871"
     CDS             complement(3102..4412)
                     /gene="fadI"
                     /locus_tag="ACHXOX_003871"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR7007231.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4592..4876)
                     /locus_tag="ACHXOX_003872"
     CDS             complement(4592..4876)
                     /locus_tag="ACHXOX_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR7007232.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5248..6588
                     /gene="fadL"
                     /locus_tag="ACHXOX_003873"
     CDS             5248..6588
                     /gene="fadL"
                     /locus_tag="ACHXOX_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR7007233.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1698 a         1826 c         1622 g         1675 t
ORIGIN      
        1 attacatcct gcctgattat tcaactctat tttctctatt atctgtatta ataaatgaca
       61 atgagtgtca aacagacatt attcatttca acatgtaact ttgggcaaaa tcaagatacg
      121 cgcagaagaa taatttccga atgaaagtag aaagtgtttt atttatagat ggggagagtc
      181 ttcgatagta gtgagattga cagatatccg ctgcctgatg agcaattatg cccaatgaat
      241 ctacctcatt gggcataatt tggaactcat cagatagctt ttgccatctt cagattacac
      301 gggctcatct gccagttgaa tgtgccgttt ccgctttcat caagcgttac gctggcaatg
      361 gccgaggtgg taaacatagg cggcgtttcg cccggacata actcggcaac cagatacccg
      421 actaacggta agtgagagat caccagtacc gaagcaacac cttcattggt caacgcctgc
      481 aaataggcac tgaccagacc aacatcgccg cagggcgtta actccggcag aacttctgca
      541 ctggaaggca ggttcagaca atcccctact tcttccagtg tttgctcggc tcgcaggaac
      601 gggctcacca gaacgcgttc gatttccact ttttgacctt tcagccagtt cgccatcagg
      661 cgagattcgt cacaaccatt agtggtcaga ggacgaaccg aatcactggc ggcatcaagg
      721 gctgcgtcgc cgtgacgcat gataaaaact tgcatattgc accgcttttg ttaaccagat
      781 tcgtccgttt tctttcatct gaaaccagag aaagaaaacg gtggccggca ttgtgcctta
      841 tccattcacc gaatgaaacg ctgtttttta cctcaatggc gtaagtatag tcaatatgcg
      901 tttacatttt gaccaacact ccgccattca gcgcggattc atatagcttt gaccttctta
      961 ttgaaggtca gttgcagttg ttttccaaaa actttcccca cgcgcgccca tctcgaccaa
     1021 acgctcgcaa ggggtaaaac gggaaccata ctgcgtggcc agtcgttgca ttattgcaac
     1081 cacttcgccc gcgccgagag aatcgatata gcggaacggt ccaccgagaa atggcggaaa
     1141 accaatgcca aataccgcgc caatatcccc gtcacgcacg ctacggataa cctgctcatc
     1201 aacacaacgt actgcttcat tcagcatcaa catcacacac cgttcagcaa cctgcggtgc
     1261 ggagagtcgc ccctgccctt gtgcgccaat gagcgggtaa atggcgggat cgacctgttt
     1321 tttgctttta cgccctttct gaccataaag atagaaaccc cgaccatttt ttctgccttt
     1381 gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc gcgctaaaac gttctccata
     1441 agcggcttcc agtacaggaa taattttagt cccggtgtcg attcctacct catccaaaag
     1501 ttggattggg cctaccggaa aaccaaattt cactagcgcg gcatcaatgt gctctacccg
     1561 ttcaccttcg gtcagcatgc ggatagcttc attaatgtaa ggcgctaaga tgcgattgac
     1621 gtaaaacccg gctttgtcac gcacgacaat gggcgttttg ccctgttttt tcgccagttt
     1681 taccgtagtg gcgatggttt gcgccgatgt acccgcatga ggaataatct ctaccagcgg
     1741 cattttttcc acaggactga agaaatgcag gccaataact tgctcaggtc gcgcggcgtg
     1801 agcggcgata tcaccaatcg gtaaagatga cgtgttcgaa gcaaaaatgg tatgagcggc
     1861 gcaattttgc tccacttccg ccaccatctg ttgttttaat tcgagatttt caaacaccgc
     1921 ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc gtcgttccgg agattaatgc
     1981 caactgtttg tcacgttcgc tggctttgag atgacgacgg cgaactttgc cctccagctg
     2041 atcccaactg tacttcagcg catgatttat gccctgcggg ttgatatctt taattctgac
     2101 cggaagcccc gctttacaag cagtgacata agcaataccg ccgcccatca agccaccacc
     2161 taaaattccc acgctgttta atggcgcagg cggtgcatca ctaccgggat ctttcttcac
     2221 gtccgtactg gcaaaaaaga tactacgcaa agcttgcgat tgcggcgtca tcgccagttc
     2281 gccaaaggcg cgagcttcag cgtcatagcc gctgctggtg ccctgcgcta atcccgtttc
     2341 aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct tgagttttgt gttctgtttt
     2401 cttgccgacc attttgaaca gcagcgcacg acctaacggc cccgccagaa tacgctcgcg
     2461 tacaggtaga gggcgggaag atgggcgatc cttcttcgcc agctcaacag cggcttccag
     2521 caaaatggag tgcggaacaa cgtcatccac cagccccagc tttaatgcct gtttcgcccg
     2581 aagttgtttt ccggtgagaa tcatctctaa tgctgtactg acgcctatca gacgcggtaa
     2641 acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt acttcaggca aaccgagcac
     2701 cgttttagga tcgtcagtac aaacgcgacc gtggcacgcc agcgccagct ccagcccacc
     2761 gcccaggcaa gcgccatgaa tagccgcgat aaccggaatg ggcaaagcat gaatctccgc
     2821 catcaactgt tggccctgcc gcgccagtgc ttccgcttct tgcgccgttt tgcagttgcc
     2881 aatcatgttg atgtctgctc cggcgataaa attgtccggt ttcgcggaga taaacaccac
     2941 gccgcgcaac tctttgtttt cacggagttg cttaataatg gcgcgcacct gcgaggcaaa
     3001 ttccgccttc agggtattca ttttctcatc cggtacatcg atggtgatga tagcaatgtt
     3061 gtccagacga acattaagag taaacgctga tgccatttcc attattccgc ctccagaacc
     3121 attgccgcgc caagcccacc ggcagcacag gcggtaacta aaccaaatcc accgccgcga
     3181 cggcgaagtt catgcaatgt ctgggtaatc atccgcgcgc cggtcgccgc gaagggatgc
     3241 ccgtaagcaa tcgaaccgcc aagcacgtta aatttgctat cgtccacttc gccagtggca
     3301 tgtgcacgcc ccagtacttc acgagcaaaa cgttcactac ccagcaactg aatattcgcc
     3361 agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg tcagttcgct catcgtcaaa
     3421 ccggcacgtt ccagcgccag cggtgttgac caggctggac cgagcaacat gtcctgccag
     3481 acatcaatcg cagtaaatgc gtagctgcgc agatacccca gcggcaccag ccctaattct
     3541 ttcgcccggg attcagtcat caggatcacc gctgccgcgc catcggtcag cggcgtactg
     3601 tttgccgccg ttaccgttcc gtgtttgcga tcaaacgccg ggcgcagctt tgcgtaatcg
     3661 gcaagcgagg aattaccgcg aatattgttg tcttcgacaa gcggttgttt ataaggaggg
     3721 ataaaggcag tcatcacctc ttctttgagt tttccgtctg accatgcctg agcggcacgc
     3781 tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg tgatgccgta ggttttcgcc
     3841 atttgttcgg cggtgtcgcc catccgcaag ccagtagaat attctgctac cgcaggcggt
     3901 acgggcatta agtcgcgcaa acgcaggcga gagaagagtt tcaggcgctg gctcatggtt
     3961 cgagctttgt tgacatcaac cagcacgcgc gccagttttt tactgacgcc aattggcaat
     4021 acggaagagg aatctgcccc accggcaatc cccgctcgga tagttcccgc catcaggctt
     4081 tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag cgcggctgac gctgtaagca
     4141 tcggtatgca cattcattcc ggtaccgaga acaatttcac gcgcaatgtt gggggcttca
     4201 ggcatttgta cgacctgacc aaagaccagc tgttcaatca cttcagcggg gatctcgctg
     4261 cgtgccagca gttcgcctac caccatcttc cctaaatcaa ccgcgggaat gccatgaaaa
     4321 gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa caatggcgat acgatcgccc
     4381 tggcgggtaa ccagcggtaa aacctgaccc ataacactcc cctgtaaaaa aataaataaa
     4441 gtggtctgac ctgatcatag tcttaactat ttttttacat ttagccaagc ggagaaaaag
     4501 gaaagtggga gctatgacac agtgaagaca ggagaaagaa aagaaaacgc ccctgccagc
     4561 aactgacagg ggcgaacgta cccgagagga attaacgcag acccagctgg aagatcagca
     4621 tttcagcttc gcaggcaaaa gtgaaatcga tatccaggcg cacaccgtca gactcttcag
     4681 tgaaagtcgg ggagattttg cacggttcgg attccacgct acgggctttt tcagtcagcg
     4741 ccgccagcgt ttgttctgct tctgcgcggt ttgcaaacac gcggctgtaa gacgcggtgc
     4801 agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg gtttcatcag
     4861 cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc agataaacgt
     4921 tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc aaaatcggat
     4981 aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa tccgcgagtg
     5041 cgcaacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg aaaaaagatg
     5101 gaaacatact tgcagcattc cagctggtcc gacctatact ctcgccactg gtctgatttc
     5161 taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac atagtttgta
     5221 taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac aaagtctgct
     5281 ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt tcagttaaac
     5341 gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat tgccgatgat
     5401 gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc gacattttct
     5461 gcgggtgcgg tttatattga cccggatgtg aatatcagcg gaacgtctcc gtctggtcgt
     5521 agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca ctttgttgca
     5581 ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg tctggcaaca
     5641 gagtttaatg atacttatgc aggcggctct gtcgggggta caactgacct tgaaaccatg
     5701 aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg tcttggtttc
     5761 aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg gcagttggtt
     5821 gctggtcaga ttatgcaatc tcctgccggg caaactcctc aagggcaagc attggcagct
     5881 accgccaacg gtatcgacag taataccaaa atcgctcatc tgaacggtaa ccagtggggc
     5941 tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta tgcactgacc
     6001 taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct taatcgtgcg
     6061 tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca atcgggttat
     6121 ctgacgctga acctgcctga aatgtgggaa gtgtcaggtt ataaccgtgt tgatccacag
     6181 tgggcgattc actatagcct ggcttacatc agctggagtc agttccagca gctgaaagcg
     6241 acctcaacca gtggcgacac gctgttccag aaacatgaag gctttaaaga tgcttaccgc
     6301 atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac cggtatcgcc
     6361 tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga ccaggaccgt
     6421 ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt cgacgttggt
     6481 gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca gttcgagtct
     6541 gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata acgcgttcgc
     6601 ctggataaag tcacctgcat agcaggtgac tttaactccc ccacttcacc gaaagtagtg
     6661 tcctcattgc ttacctcctg agttttgcaa acagcctgtt ggcagcttgc cctttcaaat
     6721 caataagcgg tatccacaga caaacctgaa gggaaaggca tattttcagg cgttctgctc
     6781 gtccttctca aagagtttac ttttctgcat ttccaggata c
//
LOCUS       DAZRVY010000179         6790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZRVY010000179 DAZRVY010000000
VERSION     DAZRVY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.180"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHXOX_003874"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHXOX_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR7007234.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHXOX_003875"
     CDS             complement(1360..1518)
                     /locus_tag="ACHXOX_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7007235.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHXOX_003876"
     CDS             2089..2238
                     /locus_tag="ACHXOX_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007236.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACHXOX_003877"
     CDS             complement(3005..3445)
                     /locus_tag="ACHXOX_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR7007237.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACHXOX_003878"
     CDS             3663..4052
                     /locus_tag="ACHXOX_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007238.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="ACHXOX_003879"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="ACHXOX_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="ACHXOX_003880"
     CDS             complement(4607..5443)
                     /locus_tag="ACHXOX_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR7007239.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="ACHXOX_003881"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="ACHXOX_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /gene="dfrA14"
                     /locus_tag="ACHXOX_003882"
     CDS             complement(5793..6266)
                     /gene="dfrA14"
                     /locus_tag="ACHXOX_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR7007240.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="ACHXOX_003883"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="ACHXOX_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1585 a         1758 c         1771 g         1676 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt ctcatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacat ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAZRVY010000180         6758 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZRVY010000180 DAZRVY010000000
VERSION     DAZRVY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.181"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(62..205)
                     /locus_tag="ACHXOX_003884"
     CDS             complement(62..205)
                     /locus_tag="ACHXOX_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR7007241.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(267..527)
                     /locus_tag="ACHXOX_003885"
     CDS             complement(267..527)
                     /locus_tag="ACHXOX_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFR7007242.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLT
                     EHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
     gene            complement(530..2275)
                     /gene="col"
                     /locus_tag="ACHXOX_003886"
     CDS             complement(530..2275)
                     /gene="col"
                     /locus_tag="ACHXOX_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HFR7007243.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNSNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEMSDADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEV
                     SKDPDLSKQFKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNI
                     RVTTPKRHIDIHRGK"
     gene            complement(2508..4727)
                     /gene="mobQ"
                     /locus_tag="ACHXOX_003887"
     CDS             complement(2508..4727)
                     /gene="mobQ"
                     /locus_tag="ACHXOX_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR7007244.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPANAPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINRDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4906..5181
                     /locus_tag="ACHXOX_003888"
     CDS             4906..5181
                     /locus_tag="ACHXOX_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFR7007245.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
     gene            complement(5397..6290)
                     /locus_tag="ACHXOX_003889"
     CDS             complement(5397..6290)
                     /locus_tag="ACHXOX_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7007246.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
     gene            complement(6521..>6758)
                     /locus_tag="ACHXOX_003890"
     CDS             complement(6521..>6758)
                     /locus_tag="ACHXOX_003890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007247.1"
                     /translation="KCHKNSMWEMGSKIPVQNFPSRGRKLVVLSRSVFRTHETDRASL
                     SCIVMQRAKNDSDAFFMFTLVFPCLFAFFASESD"
BASE COUNT         1554 a         1901 c         1571 g         1732 t
ORIGIN      
        1 aaaaaaaaag cgcatcagaa tcagcctgac acgctttgtt tttgtcaaag aggatttcgg
       61 gttactgcgt tgctaatccg gtcacttcag ctgttgatga cggagatacg gtcccgccct
      121 gaacatcccg gatatagttt gcctgacatg cagacagaat aatgactgca agaagcaata
      181 aaataatccc tgttattttt ttcataacga ctccttgttg tgaaagaatg ccagccattc
      241 atccataaac aaccggcatt catatttcag ccctgtttaa atcctgactt accgttagca
      301 gctcgccatt ctttaatttc cttgacaatc ccttcaggac tatcttccct gtcatcgcga
      361 ggataataaa tcagatctga accatctggg tgctcagtta atcgctcaaa ctctctcact
      421 aatttattgt catcctcttc agtagcacct tcagctctac atattttttt tacaaactcc
      481 agaaattcag cctcggtata atcactaata ctatgtttca gttccatttt tacttacccc
      541 gatgaatatc aatatgtcgc ttaggtgtgg tcactctgat attattcata tcatagacac
      601 caccatcctg actgattggt ttatcatgat gtaattcaaa gcgttcccta ccacctactt
      661 ggtctttctt ccttgcaaaa ggtgcttttc ccttttgaat gttcgtctta ttactgcctt
      721 taaattgctt actaagatcg ggatcttttg acacttcttc ccagaatttc ttccggaaat
      781 cgtcaaagtt tttaaattct ttatcacgca acttatcagc aatgcgatct ggaattggcg
      841 ctcctgaatc tttacctgca tcatccagcc atttatcacc aactggttta cctttacctg
      901 tcgccttccc tggcttattc cgtttactct ccttatctaa tttatcctta gcgtcctttt
      961 ctttattttc cttctgtttg cggcgctcct gcgcggcact taatgcagca tcagcatctg
     1021 acatctcttt tgcagcagca tcaaatgcag cctgcttatt atttacatcc gtctgcgccc
     1081 gctgggcttt aagcccggcc atttgccaca ttctgtgacc gccagccatt gggtcatggg
     1141 caaatcgatc aaattgtttt atttcagcta ttgcatcagc aagagtttta ttcgctgcat
     1201 caagttcgct tttacgcgaa ttataaacct gaacagcttt agcctgtcgc tcctgatttc
     1261 tggcaacatc ttcatttgcc tgattcagct ctgcacgcgc gcgttcataa tttcgctcag
     1321 ccgcttcaac cggatgcgta gcatcccatt cctgctgacg gcgattttct tcatcctgac
     1381 gttgttttac ctggtcaggg ctaagaacat cactcactga aacatatacg gcattatgac
     1441 cgctgtcctt cgggaatcga ataactgcat ccctggtatt accaccctga gtaaatcctg
     1501 ccgggcgaac atccttatca gtattatttg taacacctgg gcttaatgtc tgtactgctg
     1561 gcgtactgtt attaactgaa atattcagaa caggtgcacc tggaattgat gccgtaaaaa
     1621 cacctggacg ttcggtaggt tttgcatcaa ccaccggaac actcatcgga acacctgaaa
     1681 caaccgaaat attctgtcgt tcgtctttta catcatcaac aacacgaaca tttacgttta
     1741 ctgttgcctt atcgagaggt aatgaactga caggtgattc agtaatatca tctgcgggta
     1801 atgacgtcac aatctttgac atcatattgg ggtcatcttt cgctatttgt gatggcaata
     1861 caccatataa agccaccccc caaagaccaa atttaaacgg tcctttcagg gcagccataa
     1921 tatcagcaat agctgccgat aatgctcccg ctgaaatact gaccgccaga ccgccagctc
     1981 ctggagtgga aagtgccgga aaaccaaatg ccactggcgc agctactgct gacagattac
     2041 cgcctgttcc cgagccacca ccggaattac tattcccccc gccattacca tgaccggaac
     2101 caccacccca gtgaatgccg ctaccggaac caccacccca cgggttattt tccgaactcc
     2161 acccggaacc atcagaagca ccaccaccta caccaagccc ggtcgggcca ccattaatgt
     2221 taccacttgt gctatgcgcg cccgtgttat ggccgcgtcc atcgccaccg ctcataaaaa
     2281 ctccctcttt taagcgttaa aacaattaat tacatgatca atactgtaca taaaaccact
     2341 ggttttatgt acagatcaaa aagtacccgt catcgttttt tctgtcaagt tcaggtggaa
     2401 aattttttag aaatcaggag ggataacact tagaaaacgc agagcgggcc acaaaaaatt
     2461 ttgtggcccg tgatgtgttg gcggggtccg gggggcagcc ccggatatca catactcatg
     2521 ccccggaagt gcccggggcg gcgttgttgt tgcctgtgct gttcgtcccg cttccactca
     2581 tgccaggtct gattaaaacc gagttgacgg aagagataat ccggcatctc ttccggtttg
     2641 tggacagcag ccaaaagcct gtcatgatgg cctttgagcc ggaactgcat caggtgcggt
     2701 tccggtgggt acggagtatc ctcatcacgg tttatagctt ctccgtaacc tgccaggcat
     2761 accccgttct ccgtgatggt gctgactatc agatgactgt aatccggcga cggaagcaca
     2821 agaacggggc aaaaccctcc ttcccggcga acaaacccga caagcatttc ctccggccct
     2881 ttgcataaca acagattcag cgtcctgctg tttccctctc tcgaaaatac aacaggcatg
     2941 agttgttccg gtacagcatc agcggtgata tccacccggt gcgctgacgg cagcggagag
     3001 gctacgggcc tgcggtcgtc gctgaaataa aaattatccg gccagtgatg cggcagcgcc
     3061 ggaaaaccgt aagagggcca cccctggcgc atctgttcca gtacatgccg gaccgtcgca
     3121 ccggaataca ttctcacacg ctcaggccgc gccatcgtct catgagattc tggcattccc
     3181 tcgcgtatat ccgagaccat cacagccccg cctggcgaca ggtaaacatt caccgggtca
     3241 tgaacactct gggggtaatg cgccagctca tcaccccata ccgtgaattc tccggctctg
     3301 ttactcaaca tcatgtagcg ggttatcctg cccgttcccg gctctctggc acgccccagc
     3361 tccgtaataa ccccgactaa tgccgtttca cggctcagac ggtggttatc atgacgtttc
     3421 agccgcgcct catgctcctg ttccttccgg cgcgcttccg cttcggcttc ctccctgcgc
     3481 cgctccgctt cccgtgctga cagtttttct ttcagctctg ccattcccgg cgcaatccac
     3541 gtattccact gcgtttcaaa ggcctgacga cgggcgggaa tgtcccgttc tttcacggcc
     3601 ctgtaggtgc ggcgaagaga ttcaatctgt ttcccgagtt tccagaaacg gagttgtctt
     3661 ttgcggtatt ccggttcagc ccatgtcatc aggggaatct ctgacagaag ggacgcgaca
     3721 gggcgtgctt ttttgagtcc ggtcatctcc ttttcgagaa tggcctgctc tttcagcagc
     3781 gcattcccct cacgttcgat gtcgttcacc ttcccgctca tcgccaggaa ctccggggtc
     3841 atctcccacg cgttcccgca ttcctgcggc agcgtggcaa tgaattcccg cagggtatcc
     3901 gctgaggcat tcgcagcggg aatggtcagg ttaagggctt ccgctgcacg acgtgcggca
     3961 tcgctgagtt cccgtttccg cacgttctcc tgtacctgga tgactgccag ccttgtgcgg
     4021 gaaacctgta tttccaggtt aaggctgtta atcaggcggt tctgctcacc gcgttctgtt
     4081 tccatgcccc gtttttccat cgcacaggca gcctttccga ggtgaaggcc cggcgctttc
     4141 tccagtccct gacgctcgta tgaacgatgg tctatctctt cctggtagcc ggcgttcgca
     4201 agcgctctgt tcgcatggtc agcccatgat gcgcgccacg tctcggcgag tgcccgctcg
     4261 ttccagtccc tgacctttcc tgcaaatcct gtttccccga cagctctcgt ggtcagcatg
     4321 atgtgcgcat ggggattggt ccggtccatg tgatgaaacg ccacatcggc aatcatgccc
     4381 cgactgacaa agttttcccg gacaaaagcg agaacggcct cccgtgcggc ctcgcgggaa
     4441 atctcacggg gaatggcgag ttcaatctcc cttgccagct gggagttccg gcgctgttcg
     4501 gctttctcga ccgcattcca gagaacggaa cggtcactac accaggacgg tgcattcgca
     4561 ggggtgagaa tgaccgcatc atcaacgccg cgtttacggg tgtaatcgtg ggttaatccg
     4621 gtacggtcat cgggtatgac agagccggaa cggtaggcag cagaagcaac ggcactgtag
     4681 ccgttttttc gcgaaatgat tttcatgctg agatgataaa tcgccatttc tgccgtaata
     4741 cctttttgcc gttttctttt agcgtcgcag accgcacaca atcgaaacgg agtagagatt
     4801 gtataagtgc gcacttcgtg atgtgccgtg cattatgcca gttcagtttt gcaactacca
     4861 gtgacgcgct aaaatgcgat ttttcaccgc gcacggagtc acgaaatgag cagaacactc
     4921 gaacagaaga ttgccgaagc tgaggccaga ttacagcgcc tgaaggcgaa gagccggagc
     4981 ctggacacgg cgcaaaaagt cattgtgggg gctgccatgc tggcgagggt acggaggccg
     5041 gaagaagcgc aactgcgtgc gttcctgctc cagtttctgc gaaaggaagt cacaagacag
     5101 gcggatgtga accgactaca accgctgatt aacgagctgg agatgctacc cagaccaccg
     5161 gcgaaaccgc agaaccacta agccccggcg cgtaagtgcc catgcaccac cggacgcctt
     5221 tgcgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg agcgctacca
     5281 cctcaatcat gcggaaagcc acgccattac ggggcagaac ggagaaaatc gcaagaaagg
     5341 tcactgctca aaaagacgcc aaaagggcgc tgttatctga taaggcttat ctggtctcat
     5401 tttgcgagat ctggatcaca ttttaatttt cggtagtacg tcgctcgact gatccctaat
     5461 gcctcccacg gcttcagcga acgtgctgat gtaggaactg ctgcgcgctt agatttagtt
     5521 ccgcccttgc gacctcgagc ggcctgtaca gcggaaaatc cctctggtga gaatttcctg
     5581 tgtgtatatt tcgcaatgct cttgccaata gcccgacatt cagccggaga aagcggaacg
     5641 ggaagcgatg cgttgtacat ttcgacacgc tggatcacgg catcaagcca ttgtgagaat
     5701 acaggccaac cctgacgaat cgctctgtag gcccatttac ggaccttttc gaacagatgg
     5761 tagtttcgcc ccagcccgta atttttatcg acgctacggc gcgctgaggc gctcaaatcg
     5821 agataatcag ccagttcgtc gagagtgtag ggttcctcgc gccattccac ttcctgccat
     5881 tcagggtggc acggattttt gcagatcagg ccgctgtaat tcacatccgc gcccagtttt
     5941 tcacacaacg cacgctcaat agcggcagcg tatctgagcg ccgaagccga tgcatccggc
     6001 gcagttctca caggaagggc gagcgcgtag agcagatgcg cgtgcccgtt acggggattt
     6061 tttacggtga tgttcggggc cggacaattc cggtcgctcc agtcgattga tgcccccatc
     6121 cggtccacgt caaagggaag ccagtacagt gcatgtggct ggttctgctg gatgaaacgg
     6181 gcaagaatgg caatgttttt cggcagaatg cgaacgccgt acgcgaaatc gttcgtacag
     6241 tacggtttgt gcggtaattt ttccctgaag cgttgaagta cggcgctcat gtgatacgat
     6301 ttctctcggt acacagttta actaaccgcg ctttcccatc gggaaacgcg gaacccggcg
     6361 ccctgtaatt tcgtgatcaa tcttggcggt gagagcacga tttacggggc gtttgttttt
     6421 cagtcgctta cactacaccc aacggcaaat cctgtcgatc cttcccgaaa acccgatcca
     6481 tttaccataa ctcccgttgt acgcccccct taaaacgccc tcaatcgctt tctgacgcaa
     6541 aaaacgcaaa aagacaagga aacaccaggg taaacataaa aaatgcgtca gaatcgtttt
     6601 tagcgcgttg cataactatg cacgaaagcg atgcgcgatc ggtttcgtgc gttctgaaca
     6661 ccgaacggct caaaacgaca agttttctcc ccctggatgg aaagttctgc acggggattt
     6721 tagatcccat ctcccacatg gaatttttgt gacatttc
//
LOCUS       DAZRVY010000181         6729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZRVY010000181 DAZRVY010000000
VERSION     DAZRVY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.182"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHXOX_003891"
     CDS             38..250
                     /locus_tag="ACHXOX_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007248.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHXOX_003892"
     CDS             283..501
                     /locus_tag="ACHXOX_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR7007249.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHXOX_003893"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHXOX_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHXOX_003894"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHXOX_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR7007250.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHXOX_003895"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHXOX_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHXOX_003896"
     CDS             1917..2660
                     /locus_tag="ACHXOX_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR7007251.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHXOX_003897"
     CDS             2690..3229
                     /locus_tag="ACHXOX_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR7007252.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHXOX_003898"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHXOX_003898"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR7007253.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHXOX_003899"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHXOX_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR7007254.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHXOX_003900"
     CDS             4582..6102
                     /locus_tag="ACHXOX_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7007255.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..6729)
                     /locus_tag="ACHXOX_003901"
                     /pseudo
     CDS             complement(6135..6729)
                     /locus_tag="ACHXOX_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT         1773 a         1538 c         1589 g         1829 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaagct ctttacaggt cagcataggg cttccaaggc
     6361 tcgttgtagt gtttccggtg ttctttttac tggtagcacc gccatcattc gtggtagcgg
     6421 tctttccagt agcagccgaa acatcagtct gacctacgat tgaacgaaac tctacacctt
     6481 ggaaggtcat ctgaatacga agaccgttag caatggtatg ctctttactc gctttaaact
     6541 gagtaatgat actatcagtg atagctattc catctctaca aacaatagtg atgatttgct
     6601 tctggtctcg ccattgttca accgtagaaa taaaggtttc tacaagttgc ccctgtctgg
     6661 ttaatagaac tgaacccgtc gaatcaacca ctacaacacc gctaatagag atggtcttag
     6721 gtgctctct
//
LOCUS       DAZRVY010000182         6696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZRVY010000182 DAZRVY010000000
VERSION     DAZRVY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.183"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHXOX_003902"
     CDS             complement(<1..737)
                     /locus_tag="ACHXOX_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR7007256.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXOX_003903"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXOX_003903"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR7007257.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXOX_003904"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXOX_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR7007258.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHXOX_003905"
     CDS             4397..4678
                     /locus_tag="ACHXOX_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR7007259.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4970..5512
                     /locus_tag="ACHXOX_003906"
     CDS             4970..5512
                     /locus_tag="ACHXOX_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7007260.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5592..6266
                     /locus_tag="ACHXOX_003907"
     CDS             5592..6266
                     /locus_tag="ACHXOX_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR7007261.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6282..>6696
                     /locus_tag="ACHXOX_003908"
     CDS             6282..>6696
                     /locus_tag="ACHXOX_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR7007262.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1771 a         1513 c         1601 g         1811 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa tgatttcggc
     4741 ttttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4801 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgtttcaaa
     4861 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4921 gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta tgaaacgttc
     4981 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
     5041 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
     5101 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
     5161 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
     5221 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
     5281 cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
     5341 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
     5401 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
     5461 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
     5521 attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa
     5581 gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac
     5641 atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga
     5701 tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg
     5761 gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg
     5821 caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc
     5881 ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag
     5941 agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc
     6001 gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca
     6061 gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca
     6121 aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga
     6181 gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg
     6241 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac
     6301 ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct
     6361 ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg
     6421 atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc
     6481 gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag
     6541 ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat
     6601 ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt
     6661 tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAZRVY010000183         6553 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZRVY010000183 DAZRVY010000000
VERSION     DAZRVY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.184"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHXOX_003909"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHXOX_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR7007263.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHXOX_003910"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHXOX_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR7007264.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHXOX_003911"
     CDS             2408..3157
                     /locus_tag="ACHXOX_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR7007265.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHXOX_003912"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHXOX_003912"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR7007266.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHXOX_003913"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHXOX_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR7007267.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHXOX_003914"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHXOX_003914"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR7007268.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHXOX_003915"
     CDS             6034..6507
                     /locus_tag="ACHXOX_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR7007269.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1655 c         1659 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatgggac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAZRVY010000184         6501 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZRVY010000184 DAZRVY010000000
VERSION     DAZRVY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.185"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXOX_003916"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXOX_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHXOX_003917"
     CDS             complement(1751..2509)
                     /locus_tag="ACHXOX_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR7007270.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXOX_003918"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXOX_003918"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR7007271.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXOX_003919"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXOX_003919"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR7007272.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXOX_003920"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXOX_003920"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR7007273.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHXOX_003921"
     CDS             complement(5474..6079)
                     /locus_tag="ACHXOX_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007274.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6501)
                     /locus_tag="ACHXOX_003922"
     CDS             complement(6082..>6501)
                     /locus_tag="ACHXOX_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007275.1"
                     /translation="LQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1640 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta a
//
LOCUS       DAZRVY010000185         6372 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZRVY010000185 DAZRVY010000000
VERSION     DAZRVY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.187"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="ACHXOX_003923"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="ACHXOX_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR7007276.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="ACHXOX_003924"
     CDS             2218..2766
                     /locus_tag="ACHXOX_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR7007277.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="ACHXOX_003925"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="ACHXOX_003925"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR7007278.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHXOX_003926"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHXOX_003926"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR7007279.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHXOX_003927"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHXOX_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR7007280.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1603 a         1564 c         1613 g         1592 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat ag
//
LOCUS       DAZRVY010000186         6302 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZRVY010000186 DAZRVY010000000
VERSION     DAZRVY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.188"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHXOX_003928"
     CDS             complement(<1..273)
                     /locus_tag="ACHXOX_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007281.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
     gene            complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHXOX_003929"
     CDS             complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHXOX_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR7007282.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4551..5555)
                     /locus_tag="ACHXOX_003930"
                     /pseudo
     CDS             complement(4551..5555)
                     /locus_tag="ACHXOX_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5540..5917
                     /locus_tag="ACHXOX_003931"
                     /pseudo
     CDS             5540..5917
                     /locus_tag="ACHXOX_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5956..>6302)
                     /locus_tag="ACHXOX_003932"
     CDS             complement(5956..>6302)
                     /locus_tag="ACHXOX_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7007283.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1572 a         1408 c         1409 g         1913 t
ORIGIN      
        1 gacaatgctg tattcttttt taaagcactc accagaaacg gtgattgtaa tgataatggt
       61 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      121 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      181 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      241 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctacggtaga
      301 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
      361 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
      421 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
      481 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
      541 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
      601 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
      661 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
      721 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
      781 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
      841 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
      901 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
      961 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1021 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccactagaag
     1081 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1141 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1201 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1261 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1321 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     1381 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     1441 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     1501 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     1561 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     1621 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     1681 cagcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     1741 ccagcggtgt gtgtgtggtt ttgtccaagt tatcttcttt atactggtca acaccaaaca
     1801 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     1861 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     1921 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     1981 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2041 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2101 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2161 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2221 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2281 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     2341 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     2401 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     2461 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     2521 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     2581 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     2641 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     2701 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     2761 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     2821 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     2881 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     2941 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3001 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3061 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3121 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaattccc
     3181 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3241 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3301 ataacgagta gtttgtccag gctatattgg tactactgag aaaaccaact gtactggtaa
     3361 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     3421 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     3481 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     3541 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     3601 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     3661 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     3721 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     3781 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     3841 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     3901 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     3961 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4021 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4081 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4141 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4201 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4261 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4321 taacaaaatg aaaaaaaaag ataagcacaa gtgattgatt ggtcagatat tgatcacttg
     4381 gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     4441 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     4501 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     4561 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     4621 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     4681 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     4741 gcgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     4801 tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     4861 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacggggg
     4921 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     4981 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5041 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5101 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5161 tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc
     5221 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5281 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     5341 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     5401 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     5461 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     5521 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     5581 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     5641 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     5701 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     5761 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     5821 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     5881 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     5941 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6001 aatatcccac ataaatccag ttaactcacg agccaccgcc gtaatggcta cttgtgattt
     6061 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6121 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6181 ttttgcaggt gtacgataag accaagcggc ttcgtaaagc agacgtctca gttcgctgtt
     6241 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6301 ca
//
LOCUS       DAZRVY010000187         6190 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZRVY010000187 DAZRVY010000000
VERSION     DAZRVY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.189"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            45..1505
                     /locus_tag="ACHXOX_003933"
     CDS             45..1505
                     /locus_tag="ACHXOX_003933"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR7007284.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHXOX_003934"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHXOX_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR7007285.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHXOX_003935"
                     /gene_synonym="ydfH"
     CDS             complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHXOX_003935"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR7007286.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHXOX_003936"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHXOX_003936"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR7007287.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="ACHXOX_003937"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="ACHXOX_003937"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR7007288.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="ACHXOX_003938"
     CDS             complement(5669..6187)
                     /locus_tag="ACHXOX_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR7007289.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1544 a         1480 c         1594 g         1572 t
ORIGIN      
        1 taacaaaaca tatagcttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacta ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcactgt
//
LOCUS       DAZRVY010000188         6135 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZRVY010000188 DAZRVY010000000
VERSION     DAZRVY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.190"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHXOX_003939"
     CDS             complement(<1..1255)
                     /locus_tag="ACHXOX_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR7007290.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHXOX_003940"
     CDS             complement(1412..1621)
                     /locus_tag="ACHXOX_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007291.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHXOX_003941"
     CDS             complement(1644..2354)
                     /locus_tag="ACHXOX_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7007292.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHXOX_003942"
     CDS             complement(2870..3193)
                     /locus_tag="ACHXOX_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007293.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="ACHXOX_003943"
     CDS             complement(3372..3572)
                     /locus_tag="ACHXOX_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007294.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="ACHXOX_003944"
     CDS             complement(3572..3844)
                     /locus_tag="ACHXOX_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR7007295.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="ACHXOX_003945"
     CDS             complement(3847..4125)
                     /locus_tag="ACHXOX_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007296.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="ACHXOX_003946"
     CDS             complement(4126..4683)
                     /locus_tag="ACHXOX_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007297.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1746 a         1277 c         1070 g         2042 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgc caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gccgtatcta tcaagcattc
     5281 gcctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
     5941 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
     6001 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
     6061 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
     6121 cttaggtgct gctaa
//
LOCUS       DAZRVY010000189         6121 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZRVY010000189 DAZRVY010000000
VERSION     DAZRVY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.191"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHXOX_003947"
     CDS             <1..358
                     /locus_tag="ACHXOX_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7007298.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHXOX_003948"
     CDS             complement(398..1069)
                     /locus_tag="ACHXOX_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR7007299.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHXOX_003949"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHXOX_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR7007300.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHXOX_003950"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHXOX_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR7007301.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHXOX_003951"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHXOX_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR7007302.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHXOX_003952"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHXOX_003952"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR7007303.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHXOX_003953"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHXOX_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR7007304.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHXOX_003954"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHXOX_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR7007305.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHXOX_003955"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHXOX_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR7007306.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAZRVY010000190         6099 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZRVY010000190 DAZRVY010000000
VERSION     DAZRVY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.192"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHXOX_003956"
     CDS             <1..72
                     /locus_tag="ACHXOX_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007307.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="ACHXOX_003957"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="ACHXOX_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR7007308.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="ACHXOX_003958"
     CDS             complement(967..1311)
                     /locus_tag="ACHXOX_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR7007309.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="ACHXOX_003959"
     CDS             1463..1792
                     /locus_tag="ACHXOX_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR7007310.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHXOX_003960"
     CDS             2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHXOX_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR7007311.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHXOX_003961"
                     /pseudo
     CDS             2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHXOX_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHXOX_003962"
     CDS             2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHXOX_003962"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR7007312.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHXOX_003963"
     CDS             4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHXOX_003963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR7007313.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1539 a         1511 c         1480 g         1569 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggccccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag
     1981 agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca
     2041 tgcgcataac tatttacact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga
     2101 tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg
     2161 aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct
     2221 ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac gcggccagtg
     2281 catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg
     2341 tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga
     2401 gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg
     2461 ataggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc
     2521 ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa
     2581 atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa
     2641 cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa
     2701 acgatggatt accacgcgct gaatgcgatg cttaacctct acgatagcgc aggtcgcatt
     2761 cagttcgata aagaccgcca ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac
     2821 agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat
     2881 gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca
     2941 cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat
     3001 acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg
     3061 gtggcgctga cgctggcaca aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg
     3121 ctgtcaggac gctttcagcc agccacgccg acattcctca actgcggtaa gcagcagcgc
     3181 ggcgaactgg tttcctgttt tctgctgcgt attgaagaca atatggagtc gattggccgg
     3241 gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg
     3301 aatctgcgag aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt
     3361 ccggtgatga aaatgctgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag
     3421 ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt tctcgataca
     3481 aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc
     3541 ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat
     3601 gacgttgagc gagtttatgg caagccgttt gccgatatcg ccatcagcga acactatgac
     3661 gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag
     3721 cggctggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacggta
     3781 aaccgtgcta accctgtcgc cgggcgcata aatatgagta atctctgctc agagattttg
     3841 caggtcaact gtgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat
     3901 atctcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc cgattttgcc
     3961 cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc
     4021 agcgtgccgt ccatcgaagc cggaaatgcc gcctcacacg ccatcggact ggggcagatg
     4081 aatttacacg gctatctggc gcgagaaggc atcgcttatg gttcgccgga agcactggat
     4141 ttcaccaatc tctatttcta tgccatcacc tggcacgcac tgcgtacctc gatgttgctg
     4201 gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa
     4261 tattttagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg
     4321 tttgcccgca gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac
     4381 gtgatgcgct acggcattta caaccagaat cttcaggcgg tgccgccaac cggttctatc
     4441 tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc
     4501 aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg
     4561 gcgctgtatc aggatgctta cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa
     4621 gcgactcgcc atgtcgatca gggtttgtcg ctgacgctgt ttttccccga taccgccacc
     4681 actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat
     4741 tacattcgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt
     4801 gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata
     4861 tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa
     4921 aaggtgccgc tgtcgaacga tattcctgcc tggcagacat taactgtcgt agaacaacaa
     4981 ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc
     5041 gcgccttcgc tgatgcccga tgcactcacg cctcatgaag aagcggtatt atcgaatatc
     5101 agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag
     5161 accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa
     5221 gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt
     5281 gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc
     5341 cgcggaaagc tgaccaatac cgcagacctg atccgtctga ttatccgtga tgaagcagtc
     5401 cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa
     5461 cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagctg
     5521 caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc
     5581 tgttacaacg ccaataaggc tttgatgaat ctgggctacg aacctttatt tcccgcagaa
     5641 atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac
     5701 gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat
     5761 gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgcg acagggaaat
     5821 aattcccgcc aaatagcttt ttatcacgca aatattttgt ggtgatctac actgatactc
     5881 tgttgcatta ttcgcctgaa accacaatat tcaggcgttt tttcgctatc tttgacaaaa
     5941 aatatcaact tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
     6001 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
     6061 agggtagaaa aaagtaacta tttccattgg gtaatatat
//
LOCUS       DAZRVY010000191         6056 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZRVY010000191 DAZRVY010000000
VERSION     DAZRVY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.193"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            150..596
                     /locus_tag="ACHXOX_003964"
     CDS             150..596
                     /locus_tag="ACHXOX_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR7007314.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            607..2058
                     /locus_tag="ACHXOX_003965"
     CDS             607..2058
                     /locus_tag="ACHXOX_003965"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR7007315.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2048..3118
                     /locus_tag="ACHXOX_003966"
     CDS             2048..3118
                     /locus_tag="ACHXOX_003966"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR7007316.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3118..4866
                     /locus_tag="ACHXOX_003967"
     CDS             3118..4866
                     /locus_tag="ACHXOX_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR7007317.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4916..5956)
                     /locus_tag="ACHXOX_003968"
     CDS             complement(4916..5956)
                     /locus_tag="ACHXOX_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR7007318.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT         1430 a         1516 c         1523 g         1587 t
ORIGIN      
        1 tttatttaaa taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa
       61 attgtcattg tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg
      121 attaaaatat attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc
      181 tgaatattca gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac
      241 aaaacggttt tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa
      301 tgcactctac tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta
      361 aacaaccgtt tgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg
      421 gcgaagacgt caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg
      481 tcaaaatcat cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc
      541 aacagggcga cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa
      601 gtggcgatgg agtcatcctt atgtattgtc gcgatcacca actgccccgc cgggatcgct
      661 cacacctaca tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata
      721 aaagtggaaa ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc
      781 gctgccgatt acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg
      841 tttgccggga agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag
      901 attttcagcg aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt
      961 ggcaaacagg aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg
     1021 gcgctgccgt ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag
     1081 ttcggtttgc cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa
     1141 tccatcggtt accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg
     1201 tcgattgccg ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac
     1261 aaagcgctgc tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg
     1321 gcgattggct atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg
     1381 ctgctcggtt caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac
     1441 tacgttatcg ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc
     1501 attcctccat caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat
     1561 atgggcggcc ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac
     1621 atttatgact ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt
     1681 cttgcgacgt tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt
     1741 gccattgtgg tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct
     1801 ccgttgccga tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc
     1861 gctttcggaa tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg
     1921 atgtcgccgg tgggttcgtt ttatctggtg ctagcgattg gcctcgcgct gaacatttca
     1981 ttcattatcg tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag
     2041 cttgtccatg aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga
     2101 tgaacaggaa gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac
     2161 ctttgatggt ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt
     2221 tgtcgggcat atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct
     2281 gcgctttacc accattggcg gctggtggaa tcagtcaatg ctcaaccacc gggtaaccat
     2341 acgcacacac aagggattta aaatccctgg tgtgattggt tccgtcgcac ctcatgcgtt
     2401 aacggaaaag caaaagcaac agccgctgtc atttgatgag atgttcattg atattggcgc
     2461 gaacagtcgc gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga
     2521 agccaatttt gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat
     2581 cggctgcgca atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta
     2641 tggcgtcggc agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca
     2701 cattaaaccg gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat
     2761 tgataacatt aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa
     2821 gcgctacttc cccaaccaga aactggtggc ggcgttaaaa agctgtgccg cacataacga
     2881 tttaccgctg caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt
     2941 gatgggcggc gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa
     3001 cagcggcatg atttcaaaag ccgattacga tgctctgctc acgttgatac gggattttct
     3061 gaccacctta accgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg
     3121 ctaaacgaac gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt
     3181 gagctggcgc aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc
     3241 aactttaccc ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg
     3301 gaaatcttcg agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg
     3361 ctgctggcgc tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt
     3421 aatttgccag aaacctgggt agcagagcgt ctgccccggt taaaacagcg ttatgaacgc
     3481 acttgttgcc tggccagtcg ccccggtttg ggccatttca ttgatgagac agaagagaaa
     3541 cgcgttatct tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc
     3601 gtaacacgag acaattttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg
     3661 ctcatgcagg gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa
     3721 ctgaccgatg agtggccgca atatcccggt aacgagataa aacaaatcgt tgaacagagc
     3781 ggcatgtttc ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat
     3841 cagcaagcgc aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc
     3901 atcgcgtcat tgaatattat tgatacgcag ctggttgaga atattaccgg gcatttatta
     3961 cgttgccttg ccgcaccggt gtggattgcc gagcaccgcc aaagcagcat gaataacctg
     4021 aaagccgcct ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa
     4081 cagctcgata ttcccctttt cgatagcgat ctgatcggtt tgtattttgc ctgtgcgctg
     4141 gaacggcatc aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc
     4201 actattaatc agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt
     4261 agcttaagcg agcttattgc cattcgcgaa gagattgagc cgttattgat cattaacaac
     4321 agccattatt tactggatga cgcggtaaat aattacatca ccgtaaaaaa tatcattacg
     4381 gctgccgata tcgaacaaat aaaacacttt ctggcgacgg catttattcg ccaacagccg
     4441 gaacgctttt tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg
     4501 caacatatta cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat
     4561 gaagcgcaac ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc
     4621 gccatcccac attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc
     4681 gcccaaccag ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc
     4741 gccgatgcgc gtcatgagct aaaaatattt agctatctgg caagcgtatt gtgtcagcat
     4801 ccggcagaag ttattgccgg gttaacagga tatgaggcat ttatggagtt acttcacaag
     4861 gggtgaactg tgtcggatgc gccgcatccg acactgcatt tatcatcaaa aacgtttaac
     4921 gcaggctatt atagtcatca accatttcgt tgtactcgcg taacgctcgt ggaaatgagt
     4981 cttcgaccat ccagcttgtg tttgcatcct gaagatgctc tttatcccag tcagagtacg
     5041 ggactttatc tctgacgcgg cgaacatatt ttttagtctc gagttgatac tggtctgccg
     5101 aattaatgaa cgagaaattc ataccgcctt tatccgcttc tttggcctgc gctaccagcg
     5161 tttccagttc agagactttc ttcattgccg catcaacatc aaacttctct tgcgaaatca
     5221 ggttattaat ttgtttcgct gaaatcatga ctgccagaga gtagtagtgg aatgttttcc
     5281 cttctctttc ttcaatattt ttcagttccg caagctggcg cttatcatta atttcctgaa
     5341 tcgccgcatg ataagattct gctacaggtt caaaggattc cagatttttt aagaatgtct
     5401 ggtgcagcgt tttccctttc gcaaacgcat cgtctttata attttcctgg gtgtaatatt
     5461 tatccatttc gttaatggta ttacccaacg ccacggcagc atcaatataa ctcaccgcca
     5521 cgccatcaat aggttgcagc gcaggcgtta acgccacagc actttttacg cctttttcac
     5581 actcagcgag gttggattca ctaatgccgt agatgccata aacagtactc tctttaccgg
     5641 taggcccctg tttaaaatct ttcagccagt cagcataacg cgccagactg ttctgtacag
     5701 ggatctgtaa cttgttgtag cacttgatat agacatccat tttttcgtcg aaaaccgctt
     5761 gttcatcgac caccggttgc gcaggtgttt caggtttagc ttctgctttt gcaacgggag
     5821 cttcaggagc tggtgcggca ggttgactat tggcaggcgg gggcgtttct gtttcggctt
     5881 ttttatcatc acaacccgtc agaccgaggg ccatgatggc gactataata gcggaagata
     5941 ataaattcct tttcatttaa ttgccttata aatatataga taagagagtg aacataataa
     6001 atgaaaaaag aaaacgtcac tacacgcgtt ttatttataa ataaaataat aaaatt
//
LOCUS       DAZRVY010000192         6026 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZRVY010000192 DAZRVY010000000
VERSION     DAZRVY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.194"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            167..517
                     /gene="traA"
                     /locus_tag="ACHXOX_003969"
                     /pseudo
     CDS             167..517
                     /gene="traA"
                     /locus_tag="ACHXOX_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            532..843
                     /gene="traL"
                     /locus_tag="ACHXOX_003970"
     CDS             532..843
                     /gene="traL"
                     /locus_tag="ACHXOX_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR7007319.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            865..1047
                     /locus_tag="ACHXOX_003971"
                     /pseudo
     CDS             865..1047
                     /locus_tag="ACHXOX_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            1049..2299
                     /locus_tag="ACHXOX_003972"
                     /pseudo
     CDS             1049..2299
                     /locus_tag="ACHXOX_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2296..2682
                     /gene="trbI"
                     /locus_tag="ACHXOX_003973"
     CDS             2296..2682
                     /gene="trbI"
                     /locus_tag="ACHXOX_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR7007320.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2679..2990
                     /locus_tag="ACHXOX_003974"
                     /pseudo
     CDS             2679..2990
                     /locus_tag="ACHXOX_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            2985..3983
                     /locus_tag="ACHXOX_003975"
                     /pseudo
     CDS             2985..3983
                     /locus_tag="ACHXOX_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4003..4750
                     /gene="traX"
                     /locus_tag="ACHXOX_003976"
                     /pseudo
     CDS             4003..4750
                     /gene="traX"
                     /locus_tag="ACHXOX_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4805..5365
                     /locus_tag="ACHXOX_003977"
     CDS             4805..5365
                     /locus_tag="ACHXOX_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR7007321.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5495..5707
                     /locus_tag="ACHXOX_003978"
     CDS             5495..5707
                     /locus_tag="ACHXOX_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR7007322.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1426 a         1460 c         1837 g         1303 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg
      181 tacttctgcg cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct
      241 tcgcctggtt cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc
      301 tatggctgct ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac
      361 cttcggtaag gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc
      421 agtcatgtac atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt
      481 atttattgca gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga
      541 gacgagaata aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg
      601 tttggcctgc cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca
      661 acatcgaaat atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa
      721 ctgaaaaaag ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca
      781 gccctgctgc gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa
      841 tagaactgat accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta
      901 atggccatcg cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc
      961 attcaggggg tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg
     1021 ggatttaatg tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat
     1081 tttcttccgg ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc
     1141 cggtaaaacc gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc
     1201 cgtggtgttc gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata
     1261 tctggacggt gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc
     1321 agcggagcgt gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga
     1381 agtgcatgaa ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag
     1441 ggcacgtatt gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc
     1501 cgggtcaccg actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac
     1561 tgccaacggc acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc
     1621 aaaaatggtg gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt
     1681 gatgttctcc ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa
     1741 gaaactgaac gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg
     1801 tgagtttatt gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat
     1861 cacacagaac atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg
     1921 gggtaactcc tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa
     1981 ccagctgtat ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc
     2041 ggccaaagac cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca
     2101 ccgcctgttt gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga
     2161 gtttgtgcag cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc
     2221 gtggaagaag tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa
     2281 atacaggagt gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc
     2341 agccactggt ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc
     2401 agttacggca ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact
     2461 gtggatgcat tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc
     2521 ctttcagcgc gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac
     2581 cacgcggtca ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa
     2641 atccagcagg atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg
     2701 ccctgctgat atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt
     2761 ggccggtaaa ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc
     2821 agtccggtga gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc
     2881 tgcggccccc tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg
     2941 acccgtccgt cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg
     3001 agtcagccta cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc
     3061 gtcagggctg gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt
     3121 ttgagccgaa accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg
     3181 agctgcggga cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag
     3241 acagtcctgc acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc
     3301 cggcgtttga ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg
     3361 acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc
     3421 agttcgtggc cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg
     3481 atggcgtccg gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg
     3541 aaggccgtcc gtggaatccc ggtgccatca ccggtggccg cgtgtggggg gatatcccgg
     3601 acaacagcgt ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac
     3661 agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc
     3721 gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg
     3781 aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg
     3841 agagtgtgct gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa
     3901 atctgctgca ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag
     3961 agaaaaaccc gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg
     4021 aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg
     4081 gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg
     4141 gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga
     4201 gcctttccgc tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg
     4261 caaccggcca ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg
     4321 gcaggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg
     4381 ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc
     4441 ctgttggggg cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt
     4501 cagccaccga ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct
     4561 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct
     4621 tgtgatgacg gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt
     4681 ctggcccggg gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct
     4741 ggcgctgtga cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca
     4801 cagcatgaca gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac
     4861 gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
     4921 aaagcagaag ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca
     4981 ggccagacag gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac
     5041 cctgaaaccc tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg
     5101 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
     5161 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     5221 cggtgcctgc cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga
     5281 agcgtatgcg gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact
     5341 tcaggccgtg cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata
     5401 aggcttaaaa ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc
     5461 acaataatac tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg
     5521 gaacggccag gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag
     5581 tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac
     5641 agactggcag cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag
     5701 ggtgtaacga aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat
     5761 gagatttgct ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     5821 gagttttttc cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt
     5881 gttattcagt gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat
     5941 atggagaata acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc
     6001 caacgtggga tatttaacgc gggtta
//
LOCUS       DAZRVY010000193         5936 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZRVY010000193 DAZRVY010000000
VERSION     DAZRVY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.195"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            2..463
                     /locus_tag="ACHXOX_003979"
     CDS             2..463
                     /locus_tag="ACHXOX_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR7007323.1"
                     /translation="MFSGANLTVEVGSSAGKTVATDFVEVPEVNTFSTSGFESTVISV
                     KTFNNAYDRKLLGTKSIPDISLAVNYLPDNAVHQNLEQLADDQKRCQVKLSYFTDATK
                     KEGFFVIYTCFVSSTTIGGDKDEVVTKTFTLAVDGEAIDSGLITPKPESGE"
     gene            515..814
                     /locus_tag="ACHXOX_003980"
     CDS             515..814
                     /locus_tag="ACHXOX_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007324.1"
                     /translation="MNLNELKKKLQPELKPHEVMGEIIFIHRPNGQDFQKCTDVAKTL
                     ILCVKDENGDPIFAEEDIDGRINVNSIDFVIQNEIYSAIIALINTAKPVDEVEKK"
     gene            847..1209
                     /locus_tag="ACHXOX_003981"
     CDS             847..1209
                     /locus_tag="ACHXOX_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007325.1"
                     /translation="MVNKRGLSPEEYFSLDPDVLNMLMIYDTFIEPSGTQIEMMKHAY
                     QCYYTTISNGNLTPEARKSIKVQDFDFLDVLSDSTKSTSEKAEERKLKTKEAQSNDIK
                     SIGELIKSQVLGKKKNGK"
     gene            1199..3412
                     /locus_tag="ACHXOX_003982"
     CDS             1199..3412
                     /locus_tag="ACHXOX_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007326.1"
                     /translation="MANNNRIRVDITGDSSGLQRALRSGTESLEEFGSTAGGIVEEFT
                     GRITGMAGGFGTAMTGIAGAAAIGIGGLAALVESSREYVREMNQISKSTGLSVVQLQQ
                     LSAAFSGLGLEIDKFGDFNKDTLDKLGDAFRAGGGVSDDLKEYGLNLQDYNKYLKQAD
                     GGMQAVIHTFYAMRDAGKSQSEIVNVMETLASDSSHMISTLQQFNNEAQATAYIQSQN
                     AEVTNDAAEKYAEFDKNLGKLTTSIKGTIADGLSPLVNAMNSVYDAANQKPHEAGLFE
                     DLNERIKTSKGSLQDMLDIWEKLRTAGALNYQGAALNTGIMDDKGNKGNEFAQAKARL
                     EELANNLKSDVAVATAPTGGWVDQAKEAEDAAKKLEQQRKQAEAAQKAADAKRLQAQR
                     NLEAALAQVGEDGFQVRLQQFDRQQKALLKTITDSAQVLGINPDEMLKNATAYGAKQR
                     TDLLNSMVGYQDPNQGLKDTNALIGSGLLNDNQKGYLAQQQNQRINGGNPLAYNDTDQ
                     KLKDNADAMNAELQQNELLLKGHEDYEKRKAEITAKYNAQAIQISNQNAQDQLSIFSS
                     TAQSLSQGMVDAFGESSGAAQAAFTLSKSISIAQTVLSIQSALAQALATPWPASLANY
                     AQVLSLGMSIISTAKGAAAGQFHGGVDELPASYDNKSFVLKQGERVVQPEANKKLTAF
                     LDKQEGGSTSGDITVNAPLIIQGDVADDDKKFNEMLKKHANSVSQAVRSSQKRNT"
     gene            complement(3423..3995)
                     /locus_tag="ACHXOX_003983"
     CDS             complement(3423..3995)
                     /locus_tag="ACHXOX_003983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007327.1"
                     /translation="MLPRISSFFIYLKTSIRIFFNRLDWQAASVIVAMLLFAGQIGYN
                     AYDKWKESSDDVFKMRVILLSEINNNLLALATGGDESQVLGTYEICIPLPTEDTEAIK
                     RVATAVSYLHDDVYYSQLEKLASLPKKEVDAIVKSYYSLSRLRTLSTQLAYNIKYSPP
                     KVKLLREEYYNLYTLSSSLSQYLSYHTENP"
     gene            3997..4554
                     /locus_tag="ACHXOX_003984"
     CDS             3997..4554
                     /locus_tag="ACHXOX_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007328.1"
                     /translation="MASFSNQIKVTNFQIKSTEPIYSNQTWTGQRIMRSTGIQYYQIQ
                     FTLNFNPASLNEVNSFLAQYAQGKPFTMSLGLAGTYYGKQTGAVTATALTKPGNMVVP
                     ISTNTLAVGEWVQFSNHNKLYRIVERTSTSITIFPALQNTVQASEALKYNNLMIEAVL
                     DPDNDYSLPIGNIMNITLKATENII"
     gene            4647..5177
                     /locus_tag="ACHXOX_003985"
     CDS             4647..5177
                     /locus_tag="ACHXOX_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFR7007329.1"
                     /translation="MSLGVNVTCFDVLPKGTNGFHWTDSLIDIDLDGYKYISFPDIIS
                     GSLPSYSEQKGMSNDAINFKISNVNASVRALALGGFLKDAQMNIKLVILNPYDSTVID
                     SMLMFTGFIDYVQAVTDPNQKQNEMTIYVNSVYKKLDRQPALIAANSVYQSYYRNDEY
                     FSLLGQVNQNQNWKYK"
     gene            5177..5581
                     /locus_tag="ACHXOX_003986"
     CDS             5177..5581
                     /locus_tag="ACHXOX_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR7007330.1"
                     /translation="MKNLHNEVMSIIQYSIDNPYKYGDNDCNIIVLRLIDLINGTTQL
                     TNRQYSSIKEGIAGLNKEGWNHTGEIVEAYCKPVQATIDGDIWLDPDNPLIMAVVASG
                     RVLGVNADHDGFELQPKPTEGTYYRVRKQRDG"
     gene            5574..>5936
                     /locus_tag="ACHXOX_003987"
     CDS             5574..>5936
                     /locus_tag="ACHXOX_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HFR7007331.1"
                     /translation="MGKSLGGFFGAIITAVVVAAAVYFSGGTALAAIGWGAAAGSLSL
                     VASSMLGQIGVSGYGDVADSLSRSTSPTTGLPVIYGGELPHKNGVSGGSFILTGSIVA
                     WYNVPNSDSQYLFSEQAVA"
BASE COUNT         1959 a         1055 c         1271 g         1651 t
ORIGIN      
        1 tattttttcc ggtgctaacc ttactgtaga agtaggttca agtgctggta aaacagttgc
       61 tactgacttt gtagaagtac cagaggtaaa caccttcagt acttcaggct ttgaaagtac
      121 tgtaattagc gtaaaaacat tcaataatgc ttatgatcgt aagctattgg gtactaagtc
      181 aatcccggac atttctcttg cagttaacta cttaccagat aatgcagtac atcagaattt
      241 agaacaactt gcagacgatc agaaacgttg tcaggtaaaa ctaagctatt ttaccgatgc
      301 aactaaaaaa gaaggtttct ttgtaattta tacctgcttt gtttcaagta ccactatcgg
      361 tggtgataaa gatgaagtag taactaagac tttcaccctt gcagttgatg gtgaagcaat
      421 tgattcaggt ctgattactc caaaaccaga atcgggcgaa taataataaa taaagggaag
      481 gtgctcaact ttcccttttt tattggagat gaaaatgaat ttaaacgaat tgaagaaaaa
      541 actacaacca gaattaaaac cacatgaagt aatgggtgaa atcatcttta ttcatcgtcc
      601 aaatggtcag gatttccaga aatgcactga tgtggctaaa actctaatcc tatgtgtaaa
      661 agatgaaaat ggtgatccta tcttcgcaga agaagatatt gatggacgaa ttaatgttaa
      721 ctctattgat tttgtaatcc agaatgaaat ttactcagca attattgctt taatcaatac
      781 tgcgaaacct gtagatgaag tagaaaaaaa gtaagaggtg atatcgaatt aaagtacttt
      841 tgtaagatgg ttaacaaaag aggcttatca cctgaagaat actttagttt agatcctgat
      901 gtactcaata tgcttatgat ctacgatact tttatagaac catccggtac gcagattgaa
      961 atgatgaaac atgcgtacca gtgttactac accactatca gtaatggcaa cctaacacca
     1021 gaagcaagga aaagtatcaa ggtacaggat tttgattttc ttgatgtgct tagtgatagt
     1081 actaagagta cttcagagaa agctgaagaa agaaaattaa aaactaaaga ggctcaatcc
     1141 aatgatatta aatctatagg ggagttaatt aaatctcagg tattaggaaa gaaaaagaat
     1201 ggcaaataat aatagaatac gtgtagatat tacgggtgat tcttccggtt tacagcgtgc
     1261 tttaaggtct ggtactgaat cattagagga atttggtagt actgctggtg gcattgttga
     1321 agagttcaca ggccgaataa ctggtatggc tggcgggttc ggtactgcta tgaccgggat
     1381 cgcgggtgct gcggctattg gtattggtgg cctggcagca ttagtagaat catcacgtga
     1441 atatgtgcgt gaaatgaacc agatcagtaa gagtaccgga ttgtccgtgg ttcagttaca
     1501 acagctatca gcggcgttta gtggcctcgg tcttgagatt gataagtttg gtgatttcaa
     1561 caaggatacc ttggataagc tcggtgatgc ttttcgtgct ggcggtggtg tatccgatga
     1621 tcttaaagaa tatggactca acctacagga ctataacaag tacttaaaac aggctgatgg
     1681 tggtatgcag gctgtgatcc acacctttta cgccatgcgt gatgcaggta aatcacagag
     1741 tgaaatagtt aacgtaatgg aaacgttagc cagtgactca agccacatga ttagtactct
     1801 tcagcagttc aataatgagg ctcaagctac agcctatatc cagtcacaaa atgctgaagt
     1861 aactaatgat gctgctgaaa aatatgctga attcgataaa aaccttggga aacttactac
     1921 cagcattaaa ggtactattg ctgatggcct atcaccttta gttaatgcca tgaacagtgt
     1981 ctatgatgct gcaaatcaaa aacctcacga ggcaggtttg tttgaagatc tgaatgaacg
     2041 tattaaaact tccaagggtt ctctacagga tatgttggat atctgggaaa aattacgtac
     2101 agcaggggca ttaaactacc agggtgcagc actcaatact ggcatcatgg atgataaggg
     2161 caacaagggt aatgagttcg ctcaggctaa ggcacgttta gaagaactgg caaacaacct
     2221 aaagtccgat gttgcggttg ctaccgctcc tactggcgga tgggttgatc aggcaaaaga
     2281 agctgaagat gcagcgaaga agttagaaca acaacgtaaa caggcagaag cggcacaaaa
     2341 ggcggcagat gcaaaacgcc tacaggctca acgtaatctg gaagctgctc ttgctcaagt
     2401 tggggaagat ggttttcagg tacgtctaca gcagttcgac agacaacaaa aggcacttct
     2461 taaaaccatc accgatagtg ctcaagtact cggtatcaat cctgatgaaa tgctaaagaa
     2521 cgctactgca tatggtgcta aacaacgtac tgatttactg aattcaatgg ttggttatca
     2581 agatcctaat cagggtctga aagataccaa tgctcttatt ggttccggtc tactcaatga
     2641 caatcagaag gggtatctgg cacagcaaca gaatcagcgt atcaatggtg gtaatccatt
     2701 agcctataac gataccgatc agaagttgaa agataacgct gatgctatga atgctgaact
     2761 tcagcagaat gagttgttgc tgaaaggcca tgaggactat gagaaacgta aagctgagat
     2821 cacggcaaag tacaatgcac aggccatcca aatcagtaac cagaatgcac aggatcagtt
     2881 aagtatcttc tcaagtactg cacagtcact atcacagggc atggttgatg cgtttgggga
     2941 aagtagtggg gctgcacaag ctgcatttac cctaagtaag agcataagca ttgcacagac
     3001 agtactttca attcagtcag cattggccca agcactcgct accccttggc cagcttcatt
     3061 ggcaaactat gcacaagtac tttcacttgg tatgtcgatt attagtactg caaagggtgc
     3121 tgctgctggt cagttccatg gcggggtaga tgaactacca gcaagttatg acaacaaatc
     3181 atttgtactt aagcagggcg agagagtggt gcagcctgaa gcgaataaga aattaacagc
     3241 attcttggat aaacaagaag gtggaagtac ttcaggtgat attaccgtta acgcaccatt
     3301 gatcatccag ggtgatgtag ctgatgatga caagaaattt aatgagatgc ttaagaaaca
     3361 cgccaatagt gtttctcaag ctgtgagaag tagtcaaaaa cgtaatacat aagaaagccc
     3421 agttagggat tttccgtgtg ataagacaaa tattgtgaaa gagaagatga aagggtatag
     3481 agattgtaat actcttcccg caataattta actttaggtg gtgagtattt aatattgtat
     3541 gcaagctgtg ttgaaagagt acgtaatctc gataatgaat aataactctt tacgatggca
     3601 tctacttctt tttttgggag tgatgctaat ttttctaatt gggaataata tacatcatca
     3661 tgcaaataag aaacggcagt tgctactctt ttaatagctt ctgtatcttc agttggaaga
     3721 ggaatacata tttcatatgt gcctaacacc tgagactcat cgccaccagt agcaagagct
     3781 aaaaggttat tgttgatttc gctcaataag attacacgca tcttaaaaac atcatctgaa
     3841 ctttctttcc atttatcata tgcgttatag cctatttggc ctgcaaataa caacatagca
     3901 acgatgacgg atgcagcctg ccaatcaagc ctgttaaaaa aaatacgaat cgaggttttt
     3961 aaataaataa agaaagaaga tattcgaggt aacaaaatgg catccttttc aaatcaaata
     4021 aaagtaacga actttcaaat taaaagtact gaaccgatct attctaatca aacatggaca
     4081 ggtcaacgta tcatgagaag tactggaatt caatactatc aaattcagtt cacccttaat
     4141 tttaatcctg catcactaaa tgaagtgaat agctttttag ctcaatatgc acagggcaaa
     4201 cctttcacta tgtcattggg gcttgcaggt acatattacg gtaaacagac tggtgcagta
     4261 accgctactg ctctcactaa accgggtaat atggttgtac ctatcagtac taacacatta
     4321 gctgtgggtg agtgggtaca gttttccaat cacaataagc tatatcgaat cgtagaacgt
     4381 accagtactt caattactat tttccctgct ctacaaaata cagtacaggc aagtgaagca
     4441 ctcaaatata acaatctcat gatcgaggct gtattagatc cagacaatga ttattcattg
     4501 cctattggga acatcatgaa tattacattg aaagcaacgg agaatatcat ctaatggact
     4561 caggtatata tactaatgct aatcttctta agtactggaa gttagtcaga ggtacaacca
     4621 agacaagatt aacactaatg gaaattatga gcttaggcgt taatgttact tgttttgatg
     4681 tactaccaaa aggtactaat ggttttcact ggacggattc actaattgat atagatctgg
     4741 atggttataa gtacataagt ttccccgaca ttatcagtgg ttcattacct tcatattctg
     4801 aacaaaaggg catgtctaat gatgctatca attttaaaat tagcaatgta aacgcctcgg
     4861 ttcgtgctct tgcccttggt ggtttcttga aagatgcaca gatgaatatc aagttagtaa
     4921 ttctcaatcc atatgatagt actgtaattg attcaatgct catgtttacg gggtttattg
     4981 actatgtaca ggcagtaact gatccaaatc agaagcaaaa cgaaatgact atatatgtga
     5041 attctgtcta taagaaatta gacagacaac ctgcattaat tgccgccaac tcagtatatc
     5101 aatcttacta tagaaatgat gagtacttta gtctgttagg tcaagttaat caaaatcaga
     5161 actggaaata caagtaatga aaaatctaca taatgaagta atgagtatta ttcagtactc
     5221 aattgataac ccatataagt atggtgataa cgattgcaac attatagtac tacgccttat
     5281 tgatttaatt aatggtacta cacagttaac taatcgccag tattcgagca ttaaagaagg
     5341 tatagcaggt ttaaacaagg aaggatggaa ccatacaggg gaaattgttg aagcgtactg
     5401 taagccagta caagccacta tagacggtga tatctggtta gatcctgaca acccattaat
     5461 tatggcagta gtggcatctg gtcgggtact tggagtaaat gctgatcatg atggtttcga
     5521 acttcaacca aaaccaacag agggaacata ttacagagta aggaaacaga gagatgggta
     5581 agagtttagg gggctttttt ggagccataa ttacggcagt tgtcgtcgcg gctgctgtgt
     5641 acttttcagg aggtacagca ttagcagcaa taggttgggg tgcagcagcc ggatctttgt
     5701 cattagttgc aagttctatg ttaggtcaaa tcggggtttc tggatacggt gatgtagcag
     5761 actcactaag tagaagtact tcaccaacta ctggattacc cgttatatac ggtggagaac
     5821 taccacataa aaatggtgta tcaggcggtt ccttcatcct aaccggaagc atagtagcct
     5881 ggtacaacgt accaaattct gattctcagt acttattctc agaacaggct gtagca
//
LOCUS       DAZRVY010000194         5931 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZRVY010000194 DAZRVY010000000
VERSION     DAZRVY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.196"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHXOX_003988"
     tRNA            292..368
                     /locus_tag="ACHXOX_003988"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHXOX_003989"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHXOX_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR7007332.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHXOX_003990"
     CDS             complement(1357..2247)
                     /locus_tag="ACHXOX_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR7007333.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHXOX_003991"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHXOX_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR7007334.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHXOX_003992"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHXOX_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR7007335.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZRVY010000195         5901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZRVY010000195 DAZRVY010000000
VERSION     DAZRVY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.197"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="ACHXOX_003993"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="ACHXOX_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR7007336.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..5547
                     /locus_tag="ACHXOX_003994"
                     /pseudo
     CDS             1552..5547
                     /locus_tag="ACHXOX_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1408 a         1498 c         1910 g         1085 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg gggggctgag cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc
     2161 cgccgtaccg ggtgcttacc gggatggcgg acgctgacgt accggcgtga ggccgccggt
     2221 gacctggccg gggaaatcac cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg
     2281 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
     2341 agttcccgcc ctctctcagc ctcagcgttc cctgacacac tgcctggtac cgaatacggc
     2401 cccgacaggg gtatccgcct ttcggcggtg tggttgatgc acgacccggc atacccggag
     2461 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
     2521 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     2581 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg
     2641 gggcgggtgg tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag
     2701 gaccgcatca ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2761 gccgggctga aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg
     2821 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2881 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2941 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag
     3001 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     3061 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     3121 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     3181 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     3241 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     3301 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     3361 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     3421 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     3481 agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg
     3541 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     3601 ctcacgcaga gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc
     3661 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc
     3721 tggctgagag aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3781 gacgataagg gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3841 ctgctgtggc agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3901 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc
     3961 atgaagctgg gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg
     4021 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4081 aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg gccggataac
     4141 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4201 aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac ccaccactac
     4261 gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc actggtcgag
     4321 agccgctacc tctacgaccc gctgggacgg cgaatggcga aacgggtatg gcggcgggag
     4381 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg
     4441 gacggcgaca ggctgacgac ggtacagacc gacaccacac gtatccagac ggtatacgag
     4501 ccgggaagct tcacgccgct catccgggtc gagacagaga acggcgagcg ggagaaagcg
     4561 cagcgccgca gcctggcgga gaagctgcag caggaaggga gcgaggacgg gcacggcgtg
     4621 gtatttccgg ctgaactggt gcggctgctg gacagactgg aggaagaaat ccgggcagac
     4681 cgcgtgagca gtgaaagccg ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg
     4741 gccagacagg tggagccgga atacacacca gtgcgaaacg ttcatcttta ccactgcgac
     4801 catcggggcc agccgctggc gctcatcagc gaagacggcg atacggtgtg gagcggggcg
     4861 tatgatgagt ggggcaacca gctaaatgag gagaacccgc atcatgtgta tcagccatac
     4921 cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaatcg tcaccggtac
     4981 tatgatccgt tgcagagacg gtacatcacc caggacccca ttgggctgaa aggtgggtgg
     5041 aatctgtatc agtatccgct gaatccggtg agcaggatag atccgttggg gttattctca
     5101 tgtgagagtt gtagtcattt tatagatgat ataaataata aactggataa ccttccgttt
     5161 aacccgacaa aaatgtttac agctagttta aatactgcta attcagcaag gctgtatgca
     5221 agtggcatga caaaattcgg tataagtgtt gctgccgatg gaacgatcat tgctgcccca
     5281 gtcggtgcag cttctatggc tttaggggcg tggaatataa aatctgcgac agctgcatat
     5341 aacagagcaa atttacaatt atcagaatca cttcaggaaa atggtagaga taggtgttgg
     5401 agaaatttat tgggtatttt accttatggt gagcagtttg atgaccctgg tgaaccatca
     5461 atattagacg tttatggtga aaagattgag aatatcaaag attctccaat gaagtttttt
     5521 gaagagcttg ggacgctggg gttgtaatgt gtatttttaa tgatattttt atgtttgtgt
     5581 ttattgttag tagtatactt gtaaccgtgc attttgtgta ttctttattt tttaaaagtg
     5641 gaaaggtaaa agataaggta aacaaatgga ttaaagtaat gtgtgattta ttttggggga
     5701 taggatagtg gtgtgatatg gataaatata aattacagtg gctggcatat ttcgacctga
     5761 cgccggagta gataaaaaaa cagacaggat tggagtatag caagcgtctg acaatctctc
     5821 gggacagtag tatgatgaag aggcggggtt gtactataac cgtaaccggt actacgatcc
     5881 gctaaagggc aagtatatga c
//
LOCUS       DAZRVY010000196         5897 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZRVY010000196 DAZRVY010000000
VERSION     DAZRVY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.198"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..450
                     /locus_tag="ACHXOX_003995"
                     /pseudo
     CDS             1..450
                     /locus_tag="ACHXOX_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(492..944)
                     /locus_tag="ACHXOX_003996"
                     /pseudo
     CDS             complement(492..944)
                     /locus_tag="ACHXOX_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1008..1154
                     /locus_tag="ACHXOX_003997"
                     /pseudo
     CDS             1008..1154
                     /locus_tag="ACHXOX_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1154..2311)
                     /gene="intS"
                     /locus_tag="ACHXOX_003998"
     CDS             complement(1154..2311)
                     /gene="intS"
                     /locus_tag="ACHXOX_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR7007337.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2473..2547)
                     /locus_tag="ACHXOX_003999"
     tRNA            complement(2473..2547)
                     /locus_tag="ACHXOX_003999"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2512..2514),aa:Arg,seq:cct)
     gene            complement(2623..3555)
                     /locus_tag="ACHXOX_004000"
     CDS             complement(2623..3555)
                     /locus_tag="ACHXOX_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR7007338.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3849..4604
                     /gene="mlaA"
                     /locus_tag="ACHXOX_004001"
     CDS             3849..4604
                     /gene="mlaA"
                     /locus_tag="ACHXOX_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR7007339.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(4786..5854)
                     /locus_tag="ACHXOX_004002"
                     /pseudo
     CDS             complement(4786..5854)
                     /locus_tag="ACHXOX_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1463 a         1331 c         1303 g         1800 t
ORIGIN      
        1 tcactgtgct gaatacatag aaatttttga tgagtctatt gcttttacat gtctctctgc
       61 attttcttct gaaaaacaat ttggagtatt tctgtttgga tgtttgaaaa gcacagggct
      121 aaagttaaaa cgattattca tacggattta tctgcaccat ggcgtcttaa ggatatatca
      181 tcaagattat atctcagcga aagtttacta aagatgaaat tgaaagaaga gggggtatca
      241 ttcagtaaga tcatacttga tgagaggatg caaatggctg aatatttact cagcactcgt
      301 tgttatccta ttagtaaagt agctaaggtc tgtggttatg ccagtgtctc atactttact
      361 tatgtattta gacgttattt tggtgtttct ccaagtcaat actctcagag gagttcagaa
      421 agtaaaattc ttactcacca gggaatctga tcattgttct tgccccctta tttccagaca
      481 gggggtgtat cttaagttaa cgttacccgc tgacgtcgat attctcgcgg agagcgataa
      541 cccagcgcac tgtgcggatg gttttcattg taatgttcga tcgccactgc aagattatgc
      601 aatgccgttc ttacattcgg tttcggcatg aacgcgatgt agtcttcctt catcgttttc
      661 acgaaccttt ctgccattcc attactctga ggactgctga ttgccgttgt gcagggctcc
      721 agattcaact ctctggcgaa ctgccgtgtt tcatgcgctc tatacgctga accgttatct
      781 gtcagccact ggatggactg ttccggtact ttatcgccaa agcgtttttc tatcgctcct
      841 aacatgacat cctgcactgt cgctttatcg taacctcccg tgcttgcggc ccagtctatg
      901 gcttcacgat cgcaacagtc cagggcgaac gtaacacgca gttttgaatc gccacgggtt
      961 taacagacac ctcagagtca tttaagatgg cttaaagaga ggtgcccatg agcggtaagc
     1021 gttatcccga agagtttaaa actgaagcag tcaaacaggt tgttgatcgc ggttattctg
     1081 ttgccagcgt tgcaacacgt cttgatatca ctacccacag cctttatgcc tggataaaga
     1141 agtacggtcc ggattactcc actttttcat caagccaatc cgcccaccac tgcatcattt
     1201 ctctgcgctt atcgagatac tgagcatggt tgtaaatacc gcgcacagat ccgccgttgg
     1261 catgtgccag ctgcacttca atagcgtcag caggccattc gtgctcgttc ataatcgtgc
     1321 tgaattcatg cctgaatccg tgaccgcttt ccagaccttc atagccgatt tgtttgatca
     1381 caagcaatac cgcgttctcg cagattggct tcttcttatc gttgcgcccg gcaaaaacaa
     1441 actctgagac tggtttggtg attgagctta gcgtagtgag aagttcaacc acctggtctg
     1501 acatcgggac cacatgaatt ttgcgtccct tcatcacact ggcgtcgatg gtgataatcc
     1561 tgttttcaaa atcgacgttc ttccatagca tggaacgaag ctcttttgtt cttagggctg
     1621 tgtagcgtaa aactttggtc gcaatgagcg atacgatact tcctgaaaat gttgccagtg
     1681 ctttgttgaa tgcaggcatc tggtctgctg gaaggaacgg gaagttcttc ttgcggtatc
     1741 ctttcatggc gtctgcaagg tcaggtgccg ggttatattt agcccttccg gtgacaatag
     1801 cgtaacgaaa aacctcgccg catcttctgc gggctttgtt ggctcgctcc attgcaccgc
     1861 gatcttcaaa tctgcggatt acttccagca gttgcatcgg ctcaatatcc tgaatctcaa
     1921 gaccgccgat gatgggtaaa atgtcgtcat caaacatttt ggcaagttca gttgcatagc
     1981 ctactgacca gacttgcttc ttgtgctcgt accattcctt gtaaatcgca ctaaatgaat
     2041 tgttgttaga cgaagccttt ttcgccttta ccggaacgat gccaaccgag atgtctttcc
     2101 tcgcagtcca tgccttatcc cttgcttcct gcaaagtcat aagcggatat tttccgacgg
     2161 tcaggatttt ctccttaccg tcaatcttgt agcgaagctg ccataccttt ttcccggata
     2221 caggaacata aaggtacagg ccattaccat cgagaaggcg gtatggtttt tctttcggct
     2281 ttgctgcttc aatctgctta acggtgagca tgggtaaaaa tccggtgggt aaaattattt
     2341 tatccacttt ttacccgtca tggagtgcgg ctgtcaacga tctgacgcga accatgacga
     2401 accgtgaata tacggaaggc ttgatattca ggggattttg cggactggta cggatgggag
     2461 cgaactgata aatggtgtcc cctgcaggaa tcgaacctgc aattagccct taggaggggc
     2521 tcgttatatc catttaacta agaggacaat gcggcatgag tatacccgct aatggagtgc
     2581 ggggtaagta cgctgccgct tgattgctta aaccctcgcc atttatgccg ggtttttatc
     2641 atttttctta atgttttccg cacgttctgc tttttggcgt gcttctgctt tacgcttgtt
     2701 gctcatgtcg ttacgaatct gtgcatgact cattaacgcg aagataaagg tgccgccgca
     2761 gatgttcccc gctaaagtag gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt
     2821 accgttaaac accagataga ggatttcaac agaaccgacc acgatatggg tggtgtcacc
     2881 tagggcaata agccaggtca tcaatataat caccacaatc tttgccgcac ctgctgcagg
     2941 aaacatccat accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
     3001 gctgggggtg ttcttcatca catccatgcc gattttgaca aatgcatcgc gagtttcttc
     3061 attgaagata ggcatatatt caaatgccca cgcagcaata ccagtcccga gaatattacc
     3121 cagcagcacg actccccata accgcataag taagccaacg ttgctcattg tcggtttttg
     3181 catgacgggt agtaccgcag tcacggtgtt ttcggtaaat aattgctggc gggccattat
     3241 gacgataata aaaccaaagg tataaccgag attctccagt aagaagctgc ccggcacacc
     3301 ttccagttcg acatgaaata tcccttttgc cagtaacgaa gcgcccatcg acagacccgc
     3361 cgcaatggct gaccacagta gcgccattgc gtcgcgttcc agctcttttt caccatcctg
     3421 gcggatatgc tcatgaattg ccatcgcccg ggaggggagt cggtcttcat ctatttctat
     3481 ttttttgccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
     3541 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
     3601 tttttgctcg caactggcgg gagttactct gaaaatgtag aaaaggctgc gtttgccttt
     3661 ttctgtttct atagaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
     3721 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
     3781 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
     3841 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     3901 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg ttcaaccgca
     3961 ccatgtacaa cttcaacttc aacgtattag acccgtatat tgttcgaccg gtcgctgtcg
     4021 cctggcgtga ttatgttccg caaccggcgc gtaatggttt gagcaacttt actggcaacc
     4081 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     4141 tccactttac ccgctttttc ctgaacacca ttttggggat gggcggcttt attgatgttg
     4201 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     4261 gtcattatgg cgtgggttat gggccttacg ttcagttacc gttctacggt agcttcacgc
     4321 tgcgtgatga cggtggtgat atggcggatg gtctttaccc ggttctttcc tggctgacct
     4381 ggccgatgtc tgtgggtaaa tggacgcttg aagggatcga aacccgcgct cagctgctgg
     4441 attccgatgg tctgctgcgt cagtcgtccg atccttatat tatggtgcgc gaagcgtact
     4501 tccagcgtca tgatttcatc gctaatggcg gcgaactcaa accgcaggaa aatccgaacg
     4561 cacaagcgat tcaggatgat ttaaaagata ttgattctga ataagaaaca aataaaaaag
     4621 gtgagtcgca aaactcacct tttttgttgt tatccctcaa ttaccacaag taaaaggaga
     4681 taaaccgaca atgacccgga cgtaatgatg taaaaatact gatgttcgtc actgactatt
     4741 tccgatattc aggcgttgaa aaatcaaaaa actggaaaaa taattttaaa tatcatcaaa
     4801 attaacacct ggcgcagata gttgattaat ttcattttga tatttcgcct ggcgattttg
     4861 aagtcttttt tcaacgttat cttcgagatc cttaaagtct ttgtatttat tttcaggatg
     4921 gtcaacgtca actttgttag cgaataaatt agaaattatt tcttttacat tgtcgcgtct
     4981 aaattttgca tcgtccactt gaaaattata tcttattgct cgacctaaac gtttaaaaac
     5041 ttcgttaggt tctctcagtc tttccccgtt gagctcgttt acaacctgat gatcggaacc
     5101 tgttgcaaat gattctgcca tgatagcttt actgctttca aaccacgctg aagcattgct
     5161 gaagcattcc tggctttttc actacaatat tgggtgagac tatcaccatt aatacgaatt
     5221 cctctttgaa tgtggtagtc gtcctgatcg tagtaaccga gagataagta atcatttccg
     5281 tttttatttt taattacaat agcgagagag ttgccttttt tatctttacc gtaccaggta
     5341 aataagtccg ttggttcgct attcccgctc tcatcaaatt ccgttaccgt atgtctgagt
     5401 ttgtgtgata tcttatcgaa gataaaacca ggatctccat catatatttc ttttaatgcg
     5461 ctgaagatca tttccttgtt attgatatca tgagtcttct caactatgtt gtcattaatg
     5521 atagtacgga cgagttcttt gtctttatag ttcgcaggta attctattgg ttcttggttg
     5581 tcgataccaa tagtaatatg gtctgtatca ttggagccaa tatcaatata agtaactgag
     5641 gaattattaa tattaaatct taaggatcgt cgtttactcg tatggcgttt tttataagct
     5701 caagtagatg tttaatacag gtgcttattc cttccttacc attaaaaagg tttttgagct
     5761 cttgtactaa tccattaact tcatcattgt tattttcatt aatggattct gggtaagttg
     5821 cgctggtatt gttgattgaa atagatggta gcatgatttt attccatttt gattgatttg
     5881 ttacaaatat atattac
//
LOCUS       DAZRVY010000197         5867 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZRVY010000197 DAZRVY010000000
VERSION     DAZRVY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.199"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHXOX_004003"
     CDS             complement(38..400)
                     /locus_tag="ACHXOX_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR7007340.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHXOX_004004"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHXOX_004004"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR7007341.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHXOX_004005"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHXOX_004005"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR7007342.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHXOX_004006"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHXOX_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR7007343.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHXOX_004007"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHXOX_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR7007344.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHXOX_004008"
     CDS             5321..5683
                     /locus_tag="ACHXOX_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR7007345.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1485 a         1450 c         1519 g         1413 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaa
//
LOCUS       DAZRVY010000198         5865 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZRVY010000198 DAZRVY010000000
VERSION     DAZRVY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.200"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHXOX_004009"
     CDS             complement(<1..560)
                     /locus_tag="ACHXOX_004009"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR7007346.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHXOX_004010"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHXOX_004010"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR7007347.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHXOX_004011"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHXOX_004011"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR7007348.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHXOX_004012"
     CDS             complement(2371..3636)
                     /locus_tag="ACHXOX_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7007349.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHXOX_004013"
     CDS             complement(3863..5077)
                     /locus_tag="ACHXOX_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7007350.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHXOX_004014"
     CDS             complement(5177..5533)
                     /locus_tag="ACHXOX_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR7007351.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHXOX_004015"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHXOX_004015"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZRVY010000199         5805 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZRVY010000199 DAZRVY010000000
VERSION     DAZRVY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.201"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..257)
                     /locus_tag="ACHXOX_004016"
     CDS             complement(<1..257)
                     /locus_tag="ACHXOX_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR7007352.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAE"
     gene            353..664
                     /locus_tag="ACHXOX_004017"
     CDS             353..664
                     /locus_tag="ACHXOX_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7007353.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            753..1691
                     /locus_tag="ACHXOX_004018"
     CDS             753..1691
                     /locus_tag="ACHXOX_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7007354.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            1694..2194
                     /locus_tag="ACHXOX_004019"
     CDS             1694..2194
                     /locus_tag="ACHXOX_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007355.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            2295..2732
                     /locus_tag="ACHXOX_004020"
     CDS             2295..2732
                     /locus_tag="ACHXOX_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR7007356.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            2925..3905
                     /gene="btuC"
                     /locus_tag="ACHXOX_004021"
     CDS             2925..3905
                     /gene="btuC"
                     /locus_tag="ACHXOX_004021"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR7007357.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            3968..4519
                     /gene="btuE"
                     /locus_tag="ACHXOX_004022"
     CDS             3968..4519
                     /gene="btuE"
                     /locus_tag="ACHXOX_004022"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR7007358.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            4519..5268
                     /gene="btuD"
                     /locus_tag="ACHXOX_004023"
     CDS             4519..5268
                     /gene="btuD"
                     /locus_tag="ACHXOX_004023"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR7007359.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            5346..>5805
                     /locus_tag="ACHXOX_004024"
     CDS             5346..>5805
                     /locus_tag="ACHXOX_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR7007360.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         1465 a         1261 c         1564 g         1515 t
ORIGIN      
        1 ttctctgcgt ttctgatggt tttcaggaaa attgcgaaga gatttacttt gcgttttgtg
       61 tttcttcctt cttgagtaac gggaattacc gatctatcag cctgccttct tacagcgaga
      121 atgctttgat ttgtgcgttt cgcataatcc tctaggcttt cttcaagtac cggtagccca
      181 tgttcattac ggtatgggta gaacgccgcc aaacgctcaa aatccgcttg ttcgtatgtg
      241 ttaaggactt ttgccatggt gtgataacct attcaatctg gtgctatttg tggctctttg
      301 tagcgtcaag tggtactcaa ctgataacca atatagtatt caggtgcaca ccatgtcaat
      361 agagatatca aagaagctaa aagcaattcg agaatctgag gggcttagtc aggcaaagtt
      421 cgcggattca ataggtattg cggttggtac ggttaagcaa tacgagactg gtattcgagg
      481 tgtgggaacg gaggttttac tgaaaatcac aatgcacccg gaatttaaaa aatacactac
      541 gtggttgatg agtaacgaaa caaatgaggc tgctgggcag atcagtcctt ctctctcccc
      601 tgatgggcca gaaaacacat cgtcttctca aaaatcccgc aagactggca cacagcccgg
      661 ctaatcatgg aacgctgggg gcatggtggt cttgtaacgc tggggcttca cgaatgagca
      721 taaaatcaat tccgggaggg tatcttcttg acatgcgtcc tgaggggcgt aaaggcaaac
      781 gcattcgcaa aaaatttaaa acgaaatcgg atgcagtttt atatgagcgg tgggtgctgg
      841 cgcaacagca taacaatgag tggaaaggaa actccattga tcgccgtccg ctgtcagtgc
      901 ttattgactt gtggtggaaa taccacggcc agctaatgaa gtcagggcat aacacgcgcc
      961 ttaaattgct gcgcttgagt gaggcaatgg atgacccgtg cgtgcataaa cttaatacaa
     1021 cgatgctcac cgagctacgt gtgtccagga tagagcaggg gatacagccc agcaccataa
     1081 atcgagagat tggggcgtta agcgcgatgt ttaccgcact catctcatcc ggccattttc
     1141 ttaacgataa ccccgttcaa ggccttaaag gaatgaaggt taacgagcgt gaaatgggat
     1201 atctgagtaa gtctgaatgt gttcagttgc tggatgcact ggctgaaaat cccgatgaac
     1261 ggctggccgt cgaaatcctt ctgtcgaccg gggcgcgatg gggcgaggta gcggcactgg
     1321 agcagcgccg tgttcttcat tgtcgaatca ctttttcaaa aacgaagaac agcaaaaacc
     1381 gtaccgttcc tatttctgaa agcctgtttg aaaagatcaa aaaacggggc gggaaactgg
     1441 tgtttccgac gctggattat tcattggttc gcgatgtcat caaaacggtc gcacctgatg
     1501 ttcctgacgg ccaggctgtt catgcgctgc gccacacctt cgccagtcat ttcatgatga
     1561 acggcggcaa tattctgacg ctccagaaaa ttctggggca cgcaaagatt cagacaacga
     1621 tgatttatgc ccatcttgcg ccggattact tgcaggatgc ggtgaggttt aatcccttgg
     1681 gaggaatgta actatggaaa taaaaaaacc tacaaaaaaa gagttatatg actatttact
     1741 atcgaaatat atagaagata aatgcaaaga agaagctgat gaaatcaata agaagtcgat
     1801 gagtcgtgtc aaaaaacata aagagcggct gatggagatt acgccagaaa tcttcttccg
     1861 tttcttgtct gagaaggggg tctccagtgt ctgcccttca tgtggatcgt cgcgattatc
     1921 tgttccagaa agtatggatc tttgctggga taaaaataag aaaccagaaa attttaacaa
     1981 tctacccctg gaggaacaga gtgagttaat taaagagaac ataaagcatt atgtatctta
     2041 tgcttttttg ggtgatgtta agagcatacc tgatatgcgc aaaacttatt acacgctgca
     2101 ttgcctgaat tgtggttacc tgagccttta tcgtacgtct gcggtgctga agtggttgga
     2161 gaaagacaaa gcgcaggatg gtgataatgg gtaatgtagc aagaaatctt ttcggtaatt
     2221 cagcaggtag tgtgtcgcat tctgagcgtg atgtgcttta tcatggtggc gatggcggca
     2281 gtagtggagg cggaatgtca gataaacttg aaaggcgaat tgagcggctc gaaggtgatt
     2341 tatcgctaac aagaaacgac cttgcgacgc ttgctgaacg cactacaaac ctctcaacta
     2401 aagccgatgt tggtgaggtg aaaggtgaac tcaaagcaga catagcacat ctgaaaggtg
     2461 atcttgaatg cgatattgcg aatctgaaag gtgagcttaa atctgataca gctaacctga
     2521 aagagcagct caaatcagac attaacagcc tgaagggtga gcttaccgaa gcgatggata
     2581 aacgctttga caagattatg gatgagatga atcggcggtt tgacaaggtt gatgataata
     2641 cgaagtggcg ttggagtggc attattgtgc cagtgtgcac aaccattttc acagcggcgg
     2701 ttgctgtatt tgttgctaaa tttgttggct gatgatccac aaattgacca catctctgtt
     2761 atttgttgtg gttggctgtg tttttgtgtg tctgtaagtc tttgataatt acctaactta
     2821 ttgatttttg tttgtgttta tggccgctct gcggcctttt ttcttttcac tgtcgaagag
     2881 tcaccgtaaa atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc
     2941 gccaacaaca gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg
     3001 cgcttctctt aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc
     3061 ctcgtggcga actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg
     3121 ttggtgcggc gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg
     3181 cagaacctgg actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat
     3241 tgcttgggca agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc
     3301 ttatcatcac tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat
     3361 tgctggctgg cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact
     3421 tttccacctc tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg
     3481 tagactggcg gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt
     3541 gtcagtccag gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt
     3601 tacccctgtg gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca
     3661 gtgtggcgct ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt
     3721 tgtgtggttt aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg
     3781 cagtgctgct ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg
     3841 gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac
     3901 gttagccgca aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg
     3961 agaaacgatg caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac
     4021 cacgctggag aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg
     4081 cttaacgccg caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt
     4141 tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga
     4201 gattaaaact tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga
     4261 agttaacggc gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc
     4321 agtcgcgccg gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct
     4381 gtaccctgat gatattttat ggaattttga gaaattttta gttggcaggg acggattggt
     4441 catccagcgt ttttccccgg atatgacgcc ggaagacccc attgtgatgg aaagcattaa
     4501 actggcgttg gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc
     4561 cgcctggggc cactttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg
     4621 aatggcgcgg gtaagagtac cttactggca cgaatggccg gaatgaccag cggtaaggga
     4681 agtattcagt tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat
     4741 cgcgcctatc tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactatctg
     4801 acactgcatc agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg
     4861 gctcttgatg acaaactcgg acgtagcacc aatcaacttt ccggcggtga atggcaacgc
     4921 gtacgtcttg ctgcggtggt gctgcaaatc acaccacaag ccaatcccgc aggccaattg
     4981 ctgcttcttg atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa
     5041 attctgagcg ctctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac
     5101 cacacattgc gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt
     5161 ggacgcagag aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt
     5221 cgccgtctgg atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat
     5281 cttgaaaatc gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt
     5341 acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc
     5401 ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga
     5461 acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg
     5521 gtgtggactg ttcgggattt gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc
     5581 cccgagaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc
     5641 ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt
     5701 acgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac
     5761 ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaa
//
LOCUS       DAZRVY010000200         5640 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZRVY010000200 DAZRVY010000000
VERSION     DAZRVY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.202"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXOX_004025"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXOX_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR7007361.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHXOX_004026"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHXOX_004026"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR7007362.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXOX_004027"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXOX_004027"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR7007363.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXOX_004028"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXOX_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR7007364.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZRVY010000201         5617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZRVY010000201 DAZRVY010000000
VERSION     DAZRVY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.203"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHXOX_004029"
     CDS             complement(<1..513)
                     /locus_tag="ACHXOX_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR7007365.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHXOX_004030"
     CDS             complement(759..1211)
                     /locus_tag="ACHXOX_004030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7007366.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHXOX_004031"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHXOX_004031"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR7007367.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHXOX_004032"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHXOX_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR7007368.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHXOX_004033"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHXOX_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHXOX_004034"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHXOX_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR7007369.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5617)
                     /locus_tag="ACHXOX_004035"
     CDS             complement(5494..>5617)
                     /locus_tag="ACHXOX_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7007370.1"
                     /translation="IAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1459 g         1352 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatg
//
LOCUS       DAZRVY010000202         5614 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZRVY010000202 DAZRVY010000000
VERSION     DAZRVY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.204"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHXOX_004036"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHXOX_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR7007371.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHXOX_004037"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHXOX_004037"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR7007372.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHXOX_004038"
     CDS             2475..4124
                     /locus_tag="ACHXOX_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR7007373.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="ACHXOX_004039"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="ACHXOX_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1255 a         1271 c         1457 g         1631 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DAZRVY010000203         5613 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZRVY010000203 DAZRVY010000000
VERSION     DAZRVY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.205"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHXOX_004040"
     CDS             complement(104..418)
                     /locus_tag="ACHXOX_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR7007374.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHXOX_004041"
     CDS             complement(423..638)
                     /locus_tag="ACHXOX_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR7007375.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHXOX_004042"
     CDS             complement(789..2642)
                     /locus_tag="ACHXOX_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR7007376.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHXOX_004043"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHXOX_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHXOX_004044"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHXOX_004044"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHXOX_004045"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHXOX_004045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHXOX_004046"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHXOX_004046"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHXOX_004047"
     CDS             complement(3908..4966)
                     /locus_tag="ACHXOX_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR7007377.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHXOX_004048"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHXOX_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHXOX_004049"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHXOX_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7007378.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZRVY010000204         5541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZRVY010000204 DAZRVY010000000
VERSION     DAZRVY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.206"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHXOX_004050"
     CDS             complement(<1..1369)
                     /locus_tag="ACHXOX_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR7007379.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHXOX_004051"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHXOX_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR7007380.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHXOX_004052"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHXOX_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR7007381.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHXOX_004053"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHXOX_004053"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR7007382.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHXOX_004054"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHXOX_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR7007383.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZRVY010000205         5527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZRVY010000205 DAZRVY010000000
VERSION     DAZRVY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.207"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHXOX_004055"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHXOX_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR7007384.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHXOX_004056"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHXOX_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR7007385.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHXOX_004057"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHXOX_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR7007386.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXOX_004058"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXOX_004058"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR7007387.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXOX_004059"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXOX_004059"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR7007388.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHXOX_004060"
     CDS             4447..4812
                     /locus_tag="ACHXOX_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR7007389.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHXOX_004061"
     CDS             4962..5480
                     /locus_tag="ACHXOX_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR7007390.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZRVY010000206         5338 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZRVY010000206 DAZRVY010000000
VERSION     DAZRVY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.208"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..443
                     /locus_tag="ACHXOX_004062"
     CDS             <1..443
                     /locus_tag="ACHXOX_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFR7007391.1"
                     /translation="IKEIPVFDFDINKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEK
                     RIFVQLEYFNDVLSHTLVIDNKRGGGEVLHEIVISETSGERVYTVNEEGKTVFIASVT
                     SGTDMWSLAISRTKILDDVGLYLNLLKREKFEIIVNEDMIDWMD"
     gene            766..1140
                     /locus_tag="ACHXOX_004063"
     CDS             766..1140
                     /locus_tag="ACHXOX_004063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007392.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            1579..1764
                     /locus_tag="ACHXOX_004064"
     CDS             1579..1764
                     /locus_tag="ACHXOX_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007393.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            1754..2056
                     /locus_tag="ACHXOX_004065"
     CDS             1754..2056
                     /locus_tag="ACHXOX_004065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007394.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            2111..2665
                     /locus_tag="ACHXOX_004066"
     CDS             2111..2665
                     /locus_tag="ACHXOX_004066"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7007395.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            2787..3248
                     /locus_tag="ACHXOX_004067"
     CDS             2787..3248
                     /locus_tag="ACHXOX_004067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007396.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            3712..4455
                     /locus_tag="ACHXOX_004068"
     CDS             3712..4455
                     /locus_tag="ACHXOX_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR7007397.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            4538..5011
                     /locus_tag="ACHXOX_004069"
     CDS             4538..5011
                     /locus_tag="ACHXOX_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007398.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
BASE COUNT         1875 a          856 c         1052 g         1555 t
ORIGIN      
        1 acatcaaaga aatccctgta tttgatttcg atattaataa agtacttgat ccattgaatg
       61 tcgagttacg tgcaaagtta cggaaagaag taagattagt actgaaatct gttaagtact
      121 gggtttttga aaagcgtata tttgtacagt tggaatactt caatgatgtc ctatcccata
      181 cgcttgtgat tgataacaag cggggcgggg gtgaagtact acatgagatc gtaatctctg
      241 aaacaagcgg cgagcgtgtc tacactgtca atgaagaggg caaaaccgtt ttcattgcct
      301 ccgttacatc tggtactgat atgtggtcgc ttgccattag ccgtaccaaa atacttgatg
      361 atgttggatt gtatcttaat cttctcaagc gtgaaaaatt tgagatcatt gttaatgaag
      421 atatgattga ttggatggat taaaagctaa tgagaattta tggtcttgta gaatgaatta
      481 tgcgagttaa tatttgaaca atatatctac gatgtcgaaa tctaacggca ttgtagatag
      541 agcaataaaa gagggattat gaattttgga tataactacg cacccgatgg gtttaagatt
      601 tccgtgcaaa atacagcaaa tcatttatat cagcgtgtgt tagaatagtc aagggtaaaa
      661 ttaacttttt tgacttttca acgttaaacc agtaccatag cgtcattcac taacgaatag
      721 gagcgatgaa accatgagat gaattctaaa gagaggattg ccaaaatgcg taagaaagaa
      781 aataaagcaa aaaaagaaaa attccgtcaa tttttccctg agaattatga tattgggatc
      841 tcattgaatg aagagaatca attaaggctt ttttcaaagc ggaatgattc tcaagatgag
      901 agcttatgca agtacgaatt ttccaacaaa attaaagttc agtacatttt tttgccgtat
      961 tcttctgaaa ttcaagttat aacagatgag tttccgctaa cggaggcagg ctgtcaggag
     1021 tgtactcaag ctttcaaaca tccaatatca atggaactaa ttgaagagat aaaagaagca
     1081 aaatgttcag ttactggact tgtcatttat tcgaaaccta tactaaaact tattagctaa
     1141 cacatccaaa ttgttacgtt ttaagtactc cctcactaaa tactataaag ttgaatgtga
     1201 atgggggtac ttcaaaatgg aatggtttac taaattactt tacgagatag taggctttgt
     1261 actttacatc tttctgataa tcccaatata tatagtgctc atgtttttat ctatgatttt
     1321 agtactattg ctttccccat taaaaatcaa atcgataaat aaatacgtag taagaaaaat
     1381 gaaacgctaa aagttttggt gctatactaa tagtatcaga acgataatgc gttttgcctt
     1441 ccgctccagc tttgaacata aataatatag cgacaataaa tttccttttt aattaagatt
     1501 gcctctcttg cgagagaggt atctttttaa tttggattta taaatacagt atacaaatac
     1561 acctataagg atatagaaat gaaaaaagta actttaaagg aacgcaaaca tcatgtagta
     1621 accgcacgtc ttaatgacga tcaagtactt gtacttaacc agctaattag tagtgggaag
     1681 gtacagaaag attcattatc agcctgtgtt cagtacttaa ttaatcagta catgatttta
     1741 cagggcggtg gtaatgaaga atgactttga tttagtacaa tattttgatg attacacaat
     1801 agtaggggat atggattatc actatttcta ttgtgtgaag tatcaggaag gttcattaga
     1861 agcacattat gaacgaattg gtgatccaga agtattaggt actattacat ggaatggaaa
     1921 ttacctaatc gacggattac caatggtaat gtttgtactg gctgctgaag atgaacataa
     1981 ggaatgttta aaccgtttgc taatcgtatg tgagaaggcg agggatgaat ataggctaaa
     2041 tgctactggc ctctaagtag tagcagacgc gatcacatgg agtgacgcga taacaacaag
     2101 gacgtttgat atgaacccta aaatcacttg gagattaatt ccaaattcaa aacattacta
     2161 cgtatctgaa aatggcgatg tttacaatga taaaacaaaa cagtatatag ctacgaaaaa
     2221 cccatctaat ggatacaaaa aagtttctat taatggcaaa tcactatatg tacatgtttt
     2281 ggtttgtact ttattcaatg gtgctaaacc cattggagga agatacactg tagatcatat
     2341 cgaccgcgat aaaatgaata acaaagcaaa aaatttacgt tgggtaactc agaaggttaa
     2401 taattccaat agaaagcata aaggtactcg tactcaaaag cttactctaa agcaatggtt
     2461 aatgattgtt ggagagtacc ttaaaggaca attatctcaa tatgcaatca ctaaatggtt
     2521 tttaaatcaa actggtatta atatttgccc tcagaatata ggtaagttat tcaaaggtac
     2581 tacctattca aaatggtaca caacatatct tacgaataag caaagacaaa gaatacaaag
     2641 cctaaccatt caacgtaaac cataaaaaat gccagtattc cagtactggc atctattcaa
     2701 aactcaatgg caatgaagcc aaacttatta aatggcctgt aatcactacg ggtcacgatt
     2761 aattaattta tacaggatta tttatcatgg caaaaaccta ttcaactttt gataacagaa
     2821 acataaaagc actaattagc cagtacaagc tatataaaga tatcccatgc gaagacatca
     2881 agaacatcat gaaggactca gtagctaaac ttaaagtgag agataagatt agtgtatatg
     2941 atgtgtttgc agtattgagt aatgaagatg aattacggaa tgatttacta ttcaatacta
     3001 tcaatgctat taaagtacaa cagaataaga aacaagtaaa agaacgtatg atcaggtact
     3061 atggggcagc gatcacggat gcagcaaaac gaatagagca gtatatgttg gcacacccaa
     3121 acgcactgcc agcaccagta tatggacaaa gaacactaac atataaagag tttggtaaac
     3181 tattggaact acaaaaacaa ggtgacttta ttgatgacat gatcgagtac ttaaagggct
     3241 tgcgttaatt gtaacttatt acggcatatt acggcatgga tgtatgccgt aatacggcaa
     3301 aaaaagcccc tgaaacccgc tatttaccat caatacggca actaagtata gagatacgaa
     3361 atccattcca tttcattcca tgtatttctt tctctatcag tattccaaga aactgtgact
     3421 tgcgtcacga atactactcg tcgtgaactc ctcgtagtat tggttagtaa cgttgcgttt
     3481 ttctcgccag tactcgaaaa cccgcaaggc gttactactc gctttgctcg atagatgtta
     3541 gtacagcaaa tgaagtacct aaagtgaatt ggtaagtacg acatgccccg ccagttccac
     3601 aactctcgct ctggtcattt ccactactat gatgctattg gatagctaca taaagtactg
     3661 gatcaagtag gcattttttg tgtacaaaga gggaattaag agagggatac catgaacaga
     3721 aaacaattca tccagtcgca tggtgctact tgtagtaact ggacttggag ttggtcattt
     3781 gtgaaccacg acaagaaaat ggttatcttt ggtgcatggg atgtacaaaa agagcaagaa
     3841 agatcagtca ttctgaggga gcggtggaaa accaaagttg tggagggaaa ggaaagaaaa
     3901 aatcttggat atattcaagc tattgaacac attcaattga tagctgaagg ttatgagctt
     3961 tacacgtttg atatggagca gggacgaaac gatgagaata gagatgtagc tgttatcaaa
     4021 cggttcactc caaaacttga aaaacgttac cttcggaaag aaggaacagt ttggtatgca
     4081 gactttttac ctaatccatt tccagatgaa attacctcac ctgaaaacta cgctgagggt
     4141 gcaaaaaagc aagttacagt aaactcctac gagcgtgatc caaaagcacg acaggcttgt
     4201 attgaccatc atggtacttc ctgcgggtgt tgtgagttta actttgagaa agtctatggt
     4261 gaacacgcca agggctttat acacgttcat catatcaaac cgctacacac agtaggcgag
     4321 gattacgagg taaatccaat tactgatatg gttccacttt gtccaaactg tcacgcaatg
     4381 gtacatcgcg gtaacgaagt attaagtgtc gaaagtttaa aagcactgtt gtttaaagac
     4441 agcaataaaa tttgaattta ttttagactt gatacgacat acttgaaaag ttatgaatac
     4501 aatatgcttt aaattagaca tgtaagttga gagaaaaatg agcataacaa caatagcagc
     4561 tacatataaa gatgtgttaa ctattgcttt ttcggcgttg tcatttatta ttgcatgtac
     4621 ttccttatac ttctccggta gggcagcatg gcatgataga tcaaggctaa aaataacagc
     4681 tgacctcgtc tacgagtctt ttgatgagga accttataaa atcgaggtga tagtgatcaa
     4741 tgttggtagg cgtgatgcag ttttggaagg gatgctttgc cattatgaaa aaggcattag
     4801 gagccattca tctccaaaag agagcattgt tattaaagaa aagcaaagaa aaaaatttga
     4861 attggtaagc tcagacttag tcacgataga taatgaatgt gatatttgtc atttagaaga
     4921 tataactatt ttagatgtgg aggggaatga acataaaatc cctaactctc acagactcat
     4981 tgcattactt aagcaagata aaaagactta agaataatat tttttggaat gtaaggtaaa
     5041 tgttagatgc cactatagat tgagggttag aactactaac ccactatagg gtttacgaga
     5101 tgataagaat ggaatttaac acctacgatg gtgatatata ttacttcctc tatttatata
     5161 ggaggtacga aagggggagt actttaatga agaccttata aatcttaatg ttgagatgtt
     5221 agttatagcg gtgaggatac cccaccgtct attgggagtt gtggactcta attagagaaa
     5281 acgaataagt tattcatgtc ctttttcaag gttgcatgtg cttctattaa actatcaa
//
LOCUS       DAZRVY010000207         5282 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZRVY010000207 DAZRVY010000000
VERSION     DAZRVY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.209"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHXOX_004070"
     CDS             <1..69
                     /locus_tag="ACHXOX_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007399.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHXOX_004071"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHXOX_004071"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR7007400.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHXOX_004072"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHXOX_004072"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR7007401.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHXOX_004073"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHXOX_004073"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR7007402.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHXOX_004074"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHXOX_004074"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR7007403.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHXOX_004075"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHXOX_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR7007404.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1141 a         1398 c         1400 g         1343 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaagcg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta tcctgatgat ttgtattgaa ttatatgttt tgcgattttt
     5281 tt
//
LOCUS       DAZRVY010000208         5170 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZRVY010000208 DAZRVY010000000
VERSION     DAZRVY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.210"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            376..585
                     /locus_tag="ACHXOX_004076"
     CDS             376..585
                     /locus_tag="ACHXOX_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007405.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(651..1184)
                     /locus_tag="ACHXOX_004077"
     CDS             complement(651..1184)
                     /locus_tag="ACHXOX_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007406.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(1187..2113)
                     /locus_tag="ACHXOX_004078"
     CDS             complement(1187..2113)
                     /locus_tag="ACHXOX_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR7007407.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDGVSRENRTEPERVKSPSRGFSMGF"
     gene            2290..2688
                     /locus_tag="ACHXOX_004079"
     CDS             2290..2688
                     /locus_tag="ACHXOX_004079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007408.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEDGLTEQER
                     KLLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGQVDTK
                     TGELYYKKDKILQVLKELKYDLETSPNPDA"
     gene            complement(2883..3809)
                     /locus_tag="ACHXOX_004080"
     CDS             complement(2883..3809)
                     /locus_tag="ACHXOX_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7007409.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNSPAPNVTVKNPVNGHAHLLYALNIAVRTAPDSSV
                     KALKYAAAIERSLCEKLDADANYSGLICKNPFHLEWQVKEWREEPYSLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKIGGAKSKRG
                     AVATSARTLKPWETLGISRAWYYQLKKRGLVE"
BASE COUNT         1440 a         1340 c         1096 g         1294 t
ORIGIN      
        1 aaaaaaaaac ctctgcgagc gcagaggggg taaggaatgt tcccggtcat aatatgtcga
       61 tagtgtttat atacatacag aacaagaaca acaaccagcc aactaataaa tattttattt
      121 tttagagctg aaaccttaaa gctcacttcc attccataca ttgttataaa ccttatgttg
      181 aaaactgaaa gtaagtacgg cagacttatt aagttgcgtg cagtcttttc acgttataca
      241 aacaagaaca cctgcgaagt tatttctcct tacatatccc cctatgccaa cgggggattt
      301 tttctggttc actaacgcat aaataatagt gacaccctat cattcacaaa ccgtgataat
      361 ataaggatta tcatcatggc aaaattaggt gaaaacaccc cacatattat cgatgctact
      421 gtagatttta tggcatcaag ccaggcattt cgtgagtatc tgaaaaaact tccccctgat
      481 aacacaatac cgccagctct tactgatgag cacgtaccgt tatatatacg aaggctggaa
      541 tactatcgtc tgctttatcg accaaagcag acagaatcat attgaaaaaa acggtgaacg
      601 gttcgcgaaa tcctttcgcg ggtcgtgagg cgttggacaa gcgaagcgcg tcagtttttc
      661 cggctcttcc ccgtgacagg tttctccacc agtttatctt ccagtgtcac aactttctga
      721 tacaccgccg ctgtggcgtt gtagttcctg tcgagtattg ttttcattcc ccatccgagc
      781 acacagaaca taaccagaaa agccactgac accccaccca gccatttcca gctataccct
      841 ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc cctgttcagg
      901 gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa atcctggctg
      961 atgcgctcac ctatcagtcc tgccacgtcc agcttctgca cctcgtttct gacttcatcc
     1021 ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagagt ttcccttgcc
     1081 acagccagag aatcagcaac agctttctgt tgctctattg cggtatgttg cagatgctcc
     1141 accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag aatcccatag
     1201 aaaatccacg tgacggtgat ttaacccgtt caggttcagt tcggttctct ctgctcactc
     1261 cgtctctcat gtcaaagcca tgctcccgcg caacatcatg ttgcagcctt ctgaccagtt
     1321 catgttgctt aaccggtgta tccagccatt ccctgatgtc atgcccgacc attcgcgcct
     1381 gttcaagatg gcggtaatag tccggttcag accgtttttg catgatgtca tgacgctggg
     1441 catcatcaag tcgtccggca gcttcaaaac ccagatgcac ttcgggacgt cgttcaatgc
     1501 cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc tttctcatat
     1561 gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct ttttgctgag
     1621 tgggagtcag gcttccggaa tcctttttcg cacctccccg ctcgggatac ctggcgttat
     1681 agcgccggaa atactgttcc ggtgtacgtt caattccatc attcacacgc tgactcatca
     1741 tgatatgcgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg tgaatggcaa
     1801 aactccaggc gtgccgttcg ccggcttcct gacgtacaaa atcctgcacc agttcaaggc
     1861 gctgttccgc tgtcagttca cggggtaatg caatttcaag ttccctatag gtcgaaccgt
     1921 tcgcacgttc aaactggtca gccgcctgcc agaaatggga aggattatcc ctggcccatt
     1981 ccggcatgtt tccgtgtgcg gaatattcca aatcctgacg gtcgcggtat ttctctttgc
     2041 gctcgatgta atcggcgtgg tttgcagcct gccccttgcc accaaattta acgctgaggt
     2101 gataggttgc cacggtttca gccctctgca ggagcgcact gtttctcgac gaaggagaga
     2161 aacatataag tgcgcttcct tctaattatt cgaattagta cggaagatac cccctgcacc
     2221 cgccgaccgc aagcgatata ctgcacgacc acaaaccgcc gtaaaaaaac accagggagt
     2281 accacaaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg attcatcagg
     2341 aagctcggag aacagaccgc gcgacagaga cagattattg acctgctcga caacgaagac
     2401 ggactcacag aacaggaacg caaacttctc cacgtgctgg caacagcgga aaaaaacgac
     2461 ttacaggcac aggagaacga gcgaaaacag acgatccaga aacgaatcga gggaaaaaaa
     2521 caacgacggg aacgcaatca ccgactcttt ctcgcggctg ggctactgat cgaagcagga
     2581 caggtagaca caaaaacagg cgagctatac tataaaaaag acaagatatt acaagtactt
     2641 aaggaactta agtacgatct ggagacatcc ccaaacccag acgcgtaagt attcccgttc
     2701 acattctggt tccctggtgc ttcctcgctc attcgctcgc tacgctcggt catgagactg
     2761 cggcgagcac taccagcgca atcatgcgga aagaaacgcc attgctggga taaacgacga
     2821 aaaacctacg actcaaaagg acgcaaaaaa ggcgctgtta tctgatatag gttttatttg
     2881 gtctactcta caagacctcg ttttttcagt tggtaatacc aggcgcggct aattccaaga
     2941 gtctcccacg gtttcaacgt tcgcgcagat gtggcaacag caccacgctt agacttagca
     3001 cctccaattt tcccgccctt gcgaccacgt gcagcctgaa tttctggcgt gtgcgtatca
     3061 gccacatact gtgcgaaagt ttccggcgtg aagttcctgt gcgtgtattt cgcaatactc
     3121 ctgccaatag cccgacattc aggcggtgaa agcggaacgg gaagcgatgc gttgtacatt
     3181 tcaacacgct gaatcacggc atcaagccac tgtgagaatg ctggccagcc ctgccgaatc
     3241 gccctgtaag cccatttgcg cgtcatttcg aacagatggc agtttctccc cattccgtaa
     3301 tgtttatcga tgctacggcg cgctgaggcg ctcaaatcga gataatcagc cagttcatcg
     3361 agggagtagg gttcctcgcg ccactccttc acctgccatt ccaggtggaa cggatttttg
     3421 caaatcagcc cgctgtagtt cgcgtctgcg tcaagtttct cacacaacga gcgttcaatt
     3481 gcggcagcgt atttcagtgc cttaaccgaa ctgtccggtg cggttctcac ggcgatattg
     3541 agtgcgtaaa gcaggtgggc gtgcccgttc acaggatttt taacggtgac gttcggtgcg
     3601 ggtgagttcc ggtcgctcca gtcgatcgct gcccccacgc ggtcaacgtc aaaaaccagc
     3661 cagaactgcg catgaggctg gttctgctgg atgtaccgag caagaagcgc acgccctttg
     3721 ccggagatgc gaagaccaaa agcgagatca tccgtgtgat agggacggtg gggtaaattt
     3781 tcgtcgaagt attgaagcgc ggcgctcatg tgttacgatt ctctccgggt tatgcgacct
     3841 acacgccctc tgtatttcat gttgaatctt gccggatctg gacatgattt caggggcgtt
     3901 tgtttttcag tcgcttacac tacacccaac ggcaaatcct gtcgaacctt cccgaaaacc
     3961 cgatccattt accataactc ccgttgtacg ccccccttaa aacgccctca gtcgctttct
     4021 ggcgcaaaaa acgcaaaaag acaagaaaac accaggccaa accagaaaaa cgcgtcagaa
     4081 tcgttttcag ggcgttgcat aactatgcac gaaagcgatg tgcgatcggt ttcgtgcgtt
     4141 ctgaacaccg aacggctcaa aacgacaagt tttctcccct ggtgcgtggc gctctgacgg
     4201 ggtttcctgt ttccaaactt cacatctcgc caggacaaaa aaaaaccgac ctggggtcgg
     4261 tggcaagaat cagagtctat gggaaatatt ttagttatcg gatgctcaat attcacaaac
     4321 gcaaaaaatg cctcagtgat gccctaaaac aacctttcca gaaggagtcc gcgctttacg
     4381 atgcacggaa gagataaaaa accccacaaa agtggggaac caagagaaac aacaattaca
     4441 ggttgttggt tgtgttcgaa caacgatgaa gtagctacgc taaataataa ttaccgtaca
     4501 ggccctacat attcaacact aaatttaaga tcctgacgaa ggatttataa atgcacgaaa
     4561 acacatagac agacaaattc actaaattca tacacaaaaa caagtgtagt tttctaagta
     4621 aattagattc gacaaccaat tagtttatcc acatcgcatt ccactcttgc tacaccaatc
     4681 acatttcttg atatgtaata gcagaaaaaa taaaaccaga caccaataga ccaaaaaaca
     4741 aacattcaac aagaaacatt ttgaacaata tcacaaaact gtcacaacca tcatcagact
     4801 attgaaaaaa actggttgct gttttataaa agcgactgta acaacctcca ctctatagtt
     4861 ccattagttt aaagtgtcac acaaactgag catccatttg ttttccttac ttttgcccct
     4921 gtattccaag gggttttttt ataacttaaa gattagcaca ccacacaaaa acaataaaaa
     4981 aaacgacaag ttatacaata aaaccagtac tatccataca gctaattaca tataaatact
     5041 attaaaccat ctggacatta aaaataaact cttataacat aaaattaatg cggttccggt
     5101 accatatgtc agggataatg tgcaaatgga ggtatcggaa ctgcatttca gttcattatt
     5161 aattaatgac
//
LOCUS       DAZRVY010000209         5075 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZRVY010000209 DAZRVY010000000
VERSION     DAZRVY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.211"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHXOX_004081"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHXOX_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHXOX_004082"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHXOX_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR7007410.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHXOX_004083"
     CDS             complement(1327..3060)
                     /locus_tag="ACHXOX_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR7007411.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHXOX_004084"
     CDS             complement(3057..3551)
                     /locus_tag="ACHXOX_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR7007412.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHXOX_004085"
     CDS             complement(3673..4023)
                     /locus_tag="ACHXOX_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7007413.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHXOX_004086"
     CDS             complement(4066..4530)
                     /locus_tag="ACHXOX_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7007414.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHXOX_004087"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHXOX_004087"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR7007415.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT         1103 a         1483 c         1262 g         1227 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acaga
//
LOCUS       DAZRVY010000210         5063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZRVY010000210 DAZRVY010000000
VERSION     DAZRVY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.212"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(193..1476)
                     /locus_tag="ACHXOX_004088"
     CDS             complement(193..1476)
                     /locus_tag="ACHXOX_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR7007416.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1589..3523)
                     /gene="yeaG"
                     /locus_tag="ACHXOX_004089"
     CDS             complement(1589..3523)
                     /gene="yeaG"
                     /locus_tag="ACHXOX_004089"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR7007417.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3603..>5063
                     /locus_tag="ACHXOX_004090"
     CDS             3603..>5063
                     /locus_tag="ACHXOX_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7007418.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIE"
BASE COUNT         1315 a         1135 c         1233 g         1380 t
ORIGIN      
        1 aatatttaaa gaataacccc atggaaatac gtgttgactg aatcattttt ccgaatgatt
       61 gttttctgga actaaattat gtaaaggtta tgctgtcata tttaaagcga ttgtaagcta
      121 atgtatgtaa taaatgagat aatttacagt aagctaaaaa tcagccaggc gataatgacc
      181 tggctgatag ttttagtctt tagctgttgc attttgttta tgaaacagtt cacggaacac
      241 cggataaata tcatcctggt cgcggatgtg ctgcatcgca aagttgtcga aagtagattg
      301 cagatgctca tattctcgcc acaatgtctg atgtgcacga cgggtaattt cgatatagct
      361 gtaataacga acaacaggta ataatttttt cgccaggatt tcatggcaaa gcggagagtc
      421 atcggcccag ttatcgccgt ccgatgcttg tgcagcgtaa atattccact gtgccgggtt
      481 ataacgctct ttcactacct catccatcag tttcagggcg ctggaaacaa tggtgccgcc
      541 tgtttcctgc gagtagaaaa actcatgttc atcgacttct ttcgcctggg tatgatggcg
      601 gatgtatacg acttccacgt tcttatacgt tctgctgagg aacagataca gcagaatata
      661 aaaacgctta gccatatctt tagtggattg atccattgaa ccggaaacgt ccatcaggca
      721 aaacatcact gcctggctgg agggatcggg ctgcttctcg tagttcttgt aacgtaaatc
      781 gaaggtgtca ataaaaggga cgcgttcaat tttggcacgt aattctgcaa tttctttgcg
      841 cagacgttcc tcttccagca gttgcgcagg ttcactgttg ctgatgatgg ccaaattctc
      901 ttccagcgca tgaagttccc gtcgcttgcc cgccgtcatg gctgtgcgtc gcgccagtga
      961 gttctgcaag gaacgcacaa cgctgatatt ggctggaacg ccgttagcgg tataacccgc
     1021 ccgatgcgtt ttatattcgg tcagctggcg ttgttggttt tgtttcagat tcggtaaggc
     1081 caaatcttca aagagcagat caagatactc atctttcgaa atctgaaaga caaattcatc
     1141 ctgaccttca ccatcctggc tggcctggcc ctgaccactg ccggaacctc cgccgccacc
     1201 ctggggacgt tcaattcggt cgttctggac gaaatggtca ttgcccggat gtacgcggtg
     1261 gcgcaggccg ccacgcccct gatgaaacat cggttcgcta atatcttccg tgggaatgga
     1321 tacggattcg ccgctgtcga cgtcagtcac cgaacgctta ttaatggcct cggagatcga
     1381 ctgtttaatt tgcgctttat aacggcgtaa aaaacgctgg cgattcacca tgcttttgtt
     1441 tttgccgttc agacgccggt caataaaccc ggtcatatgc cccccgtact acatttgcca
     1501 actgtatgct acgcctgtgt tgccatttct ctggcccacg gttcgctgtc gttataggcc
     1561 cggtagcatg tgtaccgggc cagggttgtt aagacgattt acgtacgcgc aaataccatt
     1621 cgcacagtaa acgcacctgt ttacgggtgt agcctttttc catcatacgg tcgacaaagt
     1681 cgtcgtgttt cttctgctcg tcggttgacg ttttggcgtt aaacgagata accggcaaca
     1741 gctcctcggt attggagaac attttcttct cgataaccgt gcgcagtttt tcatagctgg
     1801 tccagttcgg attgcgtccg ctgttattcg ctctggcgcg cagtacgaag ttaacaatct
     1861 cgttgcggaa atcttttgga ttactgatcc ccgccggttt ctcgattttc tccagctcgg
     1921 cgttaagaga ctcgcggtca aacagctgcc cggtatccgg atcgcgatac tcctgatcct
     1981 gaatccagaa atccgcgtag gtaacataac ggtcgaaaat gttttgccca tattcggaat
     2041 aggattcaag gtaggccgtc tggatctctt tgccgataaa ctcggcatat ttcgggatca
     2101 gataaccttt caggaactcc agatagcgtt ctgcctgctc ttgcgggaac tgctcgcgct
     2161 cgatctgctg ttctaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat
     2221 cgaagttgaa cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga cctttcatcc
     2281 cttcatcgac accggcgtag tcacgatatt cctgatacga cttggctttg ggatcggtgt
     2341 ctttcagact ttcgccatca taaacccgca tctttgaata aatgctggag ttttctggct
     2401 ctttcaggcg cgaaagaatg gaaaaacgtg acagtgtttc cagcgtgcca ggggcgcatg
     2461 gggcgtgagt caattcactg tgattaagca atttctcgta gattttgatc tcttcggaaa
     2521 tgcgtaagca atacggcacc ttcacgatgt aaacacggtc gaggaaggct tcgttgtttt
     2581 tgttattacg gaaagtgacc cattcggatt cgttcgagtg ggcgagaata atcccgttga
     2641 acggcagggc ggagatacct tccgtcccgt tgtagttacc ttcctgagta gcggttaaca
     2701 gcggatgcag cactttaatc ggtgctttaa acatctcgac aaattccatg atcccctgat
     2761 tggcgcggca cagcgcaccg gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt
     2821 cgagtttacg aatatcgact ttccctacca gcgcggagat gtcctggttg ttctcatcac
     2881 cgggttctgt tttggcgata gcaatttgtt gcagaattga cggccagacc ttcactaccc
     2941 ggaacttagt gatatcgcca ccaaattcat gcaggcgttt tgccgcccac ggcgacatga
     3001 tggtgccgag atagcggcga ggaatgccat actctttttc cagaatctgc gcatcttcct
     3061 gcggattgaa aagacagaac ggatgatcgt tgaccgggct acgctcaccg ttggcgctca
     3121 atacataaat cggtacgagc tgcattaatg atttcagtcg ctcagcaagc gatgatttac
     3181 ccccacccac aggccccagc agatacagga tttgtttctt ctcttccagc ccctgagccg
     3241 cgtgtttcag ataagagaca atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa
     3301 atgccggata acgtgcaatg acccggttag aaaagagtcg agaaagtctg ggttcctggg
     3361 ctgtatcgac catgacaggc tcaccgatag ccatcaatag ccgctcagcc gcgttggcat
     3421 aagcactgcg atcttgccga caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct
     3481 tggcagcttc atatcgctgg cgatagtgat cgaatatatt catggtatgc cgtccttttg
     3541 tatactctgg gaacatttgt gaatcagcac ttttagcgta attcttcaac gggataaaaa
     3601 atatgctccg tcctatttgt tatgagcgag tatcaagcat tcagcaagtt gatatgggta
     3661 aaggtttgga cgaccaacga tcatcattga ctgcatactt agataaaaat gctgataagt
     3721 tcactgacga aaggattttc attactgatg cggggctttc cgcattcaaa aatgccaata
     3781 tctctccaga gtctcagctt gggatatttt tgcaagatgt gggcaaccgc aagtacggcg
     3841 taggtgacgc acttgtggtt ttatcgctgg atcgtctatc gcgtcgatca agctgggccg
     3901 aaagtactat ccagtacatc gtgaatagtg ggattgagat tcacgatatc tctacaaacc
     3961 ttgtgttacg ggttgatgat cctatgtcaa aaattcacat ggaactgata cagcaccgtt
     4021 cacacaatga gtcattgatg aaatcggtac gtgcaaaatt ggcatgggat aacaaaatca
     4081 cacgtgcagt acagaacggg gaagtaatct ccaaccgtat gcctaattgg ttgagaaatg
     4141 tagataacaa gtaccaagtt ataccggaac aggcagaact gattgttaaa tgtttcatgt
     4201 ggtataggga agggttgagt actggcgaga ttgttaaacg tatcggtaga aagtggcaga
     4261 tggtcactgt gtccaggctg atacgtgatc gccgtttgtt gggcgagcat gaacgttaca
     4321 acggtgaggt agtaccaaac gtttacccga aaatcatcga cgaagagttg ttcctgacag
     4381 cgaatagaat gatggatcgc gttatgttgg agaagaagaa gccagcagag gatctattac
     4441 ttgaaccaga agttgtccga gagattttta aactttatga gacaggcctt ggttcagggg
     4501 cgattgtaaa acgcttgccg aaagggtgga gtaccgtcaa tgtactgagg gtactacgtg
     4561 ataagaaagt agttaccatg aagattatcg ataacctcac ctttgaacgg gctaatgaga
     4621 agttatcgcg gaatggtata gctaatcgta tccgtaagga cattaccatt gctcaggatg
     4681 attacattac taacctcttc ccgaagatgc tgaaatgcgg tcactgtggg ggcaatattg
     4741 caattcacta taatcatgta cgaactaagt atgttatttg tcgtaacagg gaagaaaaga
     4801 agatctgcga tgctaaatct atccagtaca tgagaattga aaagaacatt ctacagcttg
     4861 tcaaaaatgt agacttcaat aaattgatga tggaaggtaa gtcggattca gattcattgc
     4921 ttgcatcatt gcgagaagag ttaagtactt tacagaaaga ggcagaaacc tatcaggtta
     4981 aaattaatga acgtaaggca gcaggtaaga aagcctcgtt accattaatg acgggtttaa
     5041 ctgaggtaca ggatagaatt gaa
//
LOCUS       DAZRVY010000211         4890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZRVY010000211 DAZRVY010000000
VERSION     DAZRVY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.214"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(146..3367)
                     /gene="carB"
                     /locus_tag="ACHXOX_004091"
     CDS             complement(146..3367)
                     /gene="carB"
                     /locus_tag="ACHXOX_004091"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR7007419.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3385..4533)
                     /gene="carA"
                     /locus_tag="ACHXOX_004092"
     CDS             complement(3385..4533)
                     /gene="carA"
                     /locus_tag="ACHXOX_004092"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR7007420.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1099 a         1360 c         1252 g         1179 t
ORIGIN      
        1 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
       61 gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa attagcggat
      121 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
      181 ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
      241 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
      301 tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
      361 cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta atgcccgctt
      421 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
      481 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
      541 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
      601 tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac agcgggtcaa
      661 cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
      721 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
      781 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
      841 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc
      901 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
      961 gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg cccgcctgct
     1021 cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
     1081 caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg ttagacacgc
     1141 tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
     1201 gaccgccgag aacgtaagac ggacgtacca ccagtgggta gccaatctct ttcgccttct
     1261 ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
     1321 caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc gggctggtgc
     1381 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
     1441 cgtactgaac gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca
     1501 gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag acggtttccg
     1561 ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagcgccagc gaggcgtgta
     1621 cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg ccgagaacca
     1681 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
     1741 tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta taaaccgggt
     1801 gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc gccagttttg
     1861 ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
     1921 cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca atctgtacca
     1981 ggaaccagcg gtcaatattg gtcaggttga acacgccgtc cacagacagg cccgcacgga
     2041 aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg cgacggattt
     2101 tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc gcaccgactt
     2161 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
     2221 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat ttttcgaagt
     2281 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
     2341 gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc agtttcgccg
     2401 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
     2461 gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat
     2521 tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
     2581 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtat
     2641 ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
     2701 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcagct
     2761 ctttggttgg ggagagatcc agaccgcggg cgcaaatttc ttcaaactct tcgcggttat
     2821 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
     2881 ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg
     2941 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
     3001 tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg ccctgacgct
     3061 ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc accgcgtccg
     3121 ggcgctcttt ttcaataatc ttgcgtacta cttcccagtg aatcggctcg atgtaggtcg
     3181 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
     3241 ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg
     3301 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
     3361 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
     3421 gataaagtgg tcgaacaacg gcgctgcgtc gtgtggccca gggctggctt ccgggtgacc
     3481 ctggaagctg aacgccggtt tatcagtgcg atgaatgccc tgtaacgtac cgtcgaacag
     3541 ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
     3601 gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc
     3661 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
     3721 accgaggcag atgccgaata ccggaatatc ggtttcgagg aatttctgga tggcggtaat
     3781 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
     3841 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
     3901 atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
     3961 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
     4021 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cattcagacc
     4081 tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc ctgcgataat
     4141 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
     4201 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
     4261 gttgctggca atcagcggca gatcgcgaat caccagacct tgtgcatgta cctgagaaga
     4321 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
     4381 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
     4441 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
     4501 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
     4561 tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc ttaccgacga
     4621 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcggt
     4681 attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaagatc
     4741 atataacctg ttctggaaaa cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc
     4801 aggaaaaagt ggaccaaatg gtcaaaattt acattaaaac aacaaaaaca gaagacaaaa
     4861 catgtaattt ttggcactta tagaatattt
//
LOCUS       DAZRVY010000212         4882 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZRVY010000212 DAZRVY010000000
VERSION     DAZRVY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.215"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..2202)
                     /locus_tag="ACHXOX_004093"
     CDS             complement(<1..2202)
                     /locus_tag="ACHXOX_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7007421.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAAN"
     gene            complement(2183..2596)
                     /locus_tag="ACHXOX_004094"
     CDS             complement(2183..2596)
                     /locus_tag="ACHXOX_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR7007422.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(2623..3054)
                     /locus_tag="ACHXOX_004095"
     CDS             complement(2623..3054)
                     /locus_tag="ACHXOX_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR7007423.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(3068..3820)
                     /locus_tag="ACHXOX_004096"
     CDS             complement(3068..3820)
                     /locus_tag="ACHXOX_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7007424.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(3828..4223)
                     /locus_tag="ACHXOX_004097"
     CDS             complement(3828..4223)
                     /locus_tag="ACHXOX_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR7007425.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(4220..4795)
                     /locus_tag="ACHXOX_004098"
     CDS             complement(4220..4795)
                     /locus_tag="ACHXOX_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7007426.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(4810..>4882)
                     /locus_tag="ACHXOX_004099"
     CDS             complement(4810..>4882)
                     /locus_tag="ACHXOX_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR7007427.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT          977 a         1557 c         1172 g         1176 t
ORIGIN      
        1 gttcgccgcc gcagcctgaa tggccgtccc cgtggaggca gacgcaccac caccgaaagc
       61 accgccaatg gcgctgccga tactcccgac tatccccacc atcgcctgct tcagaaaaat
      121 ctctgtcagc atggacagca cagaacgggt gaaaccacgc cagttctgtt cgctgccgat
      181 cagcatcgct gccatattct gtgcaatacc gtcaaaggtc tgcgtggccg cgtttttaac
      241 ctgcgaaaaa ctgtccgtcg cactttccgc ccactcgccc cagccggact tcatcccggc
      301 catccagctt ccacgaagct gctcctccgc agaccaggtg ttcttcagtg cagatgtggc
      361 cttcgccagc gcagccggat tatcaccgta cacctcacga aggcgctgct cttccgactc
      421 ccgctgcgcc tgacggtcgg tgagtccgcg ggcttttgcg ctgattgccg cctgcttcgc
      481 gctctgctgc tgttcaaacc gcgccgcctg ctgtgccagc tcattcagcc gtttctggtg
      541 ttcaatcttg tcgcccagct cagccagctg gcgtttgtac tccagcgttt ctttctcatg
      601 ggccagcagg gatttttcct gctcagataa ctgccgtttc gtggcggcct ctttcaggac
      661 cacatactga ttttccgctt tccataaatc acggcgctgc tggctgattt tttcattcgc
      721 accgctgtgc ttctccagcg tcctgagctc agtttcaagc gccagcaggg ctgcatgcgc
      781 ctggtcttcc tggcgctcac cggcagacac cttcacaccg gacggctttt tctgcgtcga
      841 ctcataatcc ttttttgccg acgccatcag cgtgttgtaa tccgcctgca ggattttccc
      901 gtctttcagg gccttattca gctcttcctg ccgggcggta tatttctcca gtggcgtcag
      961 caggcgctca tacgccttct gcgcctctcc ggtatacttc agctgtgacg actcacgctc
     1021 agccctgtcc cttgccgcca gttcaccggc tttttccata tccgactgca gcgttgccgc
     1081 tgccagaccc agacgggcat tttcccggtc atcccatgcg ccctgaaggt tggcccggaa
     1141 agaggaggtt ttaccgcggc gctggctccg actctggtac cactgccatt ttttatccgc
     1201 ctcatcaaat gccttctgcg cactggcgag catatccgct gaggattccg gacgaccgat
     1261 atccagaatg gcatcccaca tcgatttgaa tgccttccct gttttatccg cccaggtctc
     1321 cagtgttccc atgttttctt tcaggcgacg ggtctgctca tcaaagcctt tcgtggcgat
     1381 atcgttcgcc gcctgcaatg ccccggcctc gtctccggaa cgctgcagct gtgcaacata
     1441 cgcaatctgc tctgccgtca cgttacggaa ctggcgcgcc atcgccatca gtcccgacgt
     1501 cgggtcagtg gtcagcttcc cgaaggcttc agcgacttta tccacctcca caccggatgc
     1561 agacgcaaaa cgcgcgacac tctggttgat ggcatcaaac tgttcaccac cacgcacacc
     1621 ggcattcacc agggctgcca gtgactctct cgcctggtta aacgtcagcc ctgctgcctg
     1681 cccggctctt gagagagtca gcatacgatc ggcagtcagt ccggactgat taccggaaag
     1741 aaccagggtt ttattaaacg ctgaaagcgt ggaatctccc tggtaccagg cgtacaccag
     1801 tgcacctgtc gccaccgcca gcgaggtgac cccgaccatc ggcagggtga tcgcatcggc
     1861 aagccccctg aacatgggga tcatcccgcc gaaggagtcc ttcacctgac cgccctgttg
     1921 cagcaggatc agccagggat tctgaccacc ggcaagctgc gtggcgatat ccgtaaactg
     1981 tgcgggcagg gttcgcatgg ccgctttata ctgcccgacg gaaatcccgg ctttttgtgc
     2041 agccagcgcc tggcggctca ggccctgttc aacagcactg gcggtttttc tggcgtcggt
     2101 atccagacct gaaaaatgac gccttacccg gctcatctgc tcatcgaaac ggacagcatc
     2161 cagactcagg tcaataacaa gatcaccaac cggctgggac atatctcaca cctcccggaa
     2221 tccccgctga agccatcatt aatgcggcat catccaccat gacatccgcc acatccgcag
     2281 acgataaaat atcgcgccct ccgtccccac cgaaccggac gcctccggca agtcctgccg
     2341 ctttctgcat cagcattttg tcctcatccg gcctctccac ctgctcttcc tcatgccggg
     2401 ggacaagcag actgaaatca gagggatgca tatccggatc gcaaaaaaac aggctgagta
     2461 cagcgtacgt cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc
     2521 ggtaaaaacg gcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc
     2581 agtcgggtct ccccatctca cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga
     2641 ctttcccgca gaaaaatcat catcagtcag cgtgttattt ttcgccactt cagtaatatc
     2701 agtatccgga cgaacagctt cgatcatccc ggacaggcac aacaccacgt cttccgcccg
     2761 ggcaatggca tcggcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc
     2821 attcattgac ggtgactgcg ttttctgtgg atggttatgc cacagggaca tcgccaccag
     2881 aaacgcgccg gttctgacga gatcttccac gcttacctgc aggttgccgc tggattctgc
     2941 ctgttctgca cgccgtttca ggagggcaag atgctcgata cgctgcagcg cagacaattc
     3001 ggaaagcgtg acagacacac cgttatattc aaattgttct gttttcagaa acatgtatta
     3061 cctccgttta ccctgcagcg cccgcttcag taacggtgac ttcagccact gcggcgaact
     3121 gaccatttcc gctcaccaca gggatctgca ccttacctgt cgccacgccg tttaccgtaa
     3181 ttgtcatatc tttcacacta atggtggctt tcgacggatc ggcggaaacc gctctgaacg
     3241 tcttgtcggt tgcactttcc ggctcaaaag aaaccgtcag ggtggttgtt ttcccttttg
     3301 ccaccgtacc ggatgtcggc gtcaccttaa tcgcactgac cggcgtaatt ttgctgcgtt
     3361 cttccgctac agaaggttta cccacgttag tgactttcac cgtgcgggtg atcacttctt
     3421 tcgccgtcac ggccttaccg atactgctga cccagccacg aaacacatcc accgtgccat
     3481 tcggaaaacg gattttatag gcccggacat cgccgctttc aaaccagcct ataagccctt
     3541 tctggccttc ctctcccggt ttccaggcca gcgtaaaact ggtatcacct gcagatttct
     3601 gcccctgccc ggtcgcgctc cagtccgcgt cttcatcatc caggtagtta tcatcatagg
     3661 attcagccgt catctcgccc ggcgtcagat ccttcacctt agccagtcgc tgccagtcat
     3721 cgtctgacaa cgggtttgca taagcatcag ccttgccgtt gtaaacccac agagtggtac
     3781 cggcaccttt taccggctcc aggggatttg gtgttgccat atcgtcctca catctcgtat
     3841 gtaatggaat aagtcagatc cgcagagctc cataacgcca tatcgtcatc acgacgatac
     3901 tcatagccct gcgtaaccat cgtggtaatc agtcctgcca gtgcagggat cgcagtcatc
     3961 gccggataaa tccggctttc catccactga tcgagctccg aatcaggtac ctgtgccggt
     4021 aaaaacacct caatatgcag tgtggcccgc caggtatctg catccagctc ttcaccggta
     4081 tactctgcat ccgtcagata aaccgcgatc gcaggaaaat cctcttcgtc aaaaacaacg
     4141 gggcgaccat caaacagcgt cgccccgtgt tcatgctgct cgagtgcatc cagcactgcg
     4201 gcacgaatgt cagtgtgttt catcgtttta tcgcaatcct cagttgttgt ttcagcgcgt
     4261 atgccagttc tttaggcagg cgttcacgcc ggatacggtc aacattctca tcaaatgcct
     4321 gtttcagtgg ggccgccatc gggattttca ccacctgaat gggaaggcga ttacgctttt
     4381 tccttccctt gtcgtcattg ccctcctcat atctggcctg gggaagacgt tgcataacat
     4441 gccagcgccc attatttaat cgctggataa atgcccgctg ataacgatgc tgaccggctt
     4501 tgagtatgct gttcggacga cgccccagca ttctgatccc cagcttaatc acagggagat
     4561 caccgcggtt aacgataatt ctggcattcg gatttctgac cgtcgcccgt ttcagtctgg
     4621 accgttcctt taccagtttc cgtctcaccc tggtttcccg ggcaacctgt gacgaagact
     4681 gattaatcgc cgttgtggcc acgcggttaa tggtcattgc agaagcagcc ggaatggcgt
     4741 ttttacgaac ccggttcaga ttgtcaatcg cctgatcaag cccttttatc gccataattc
     4801 accctgcgtt tatcgtcgcc ggttaactgc gggtggttgc ccacggttga gccagagata
     4861 acagctgccc ccgtcatccg ga
//
LOCUS       DAZRVY010000213         4830 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZRVY010000213 DAZRVY010000000
VERSION     DAZRVY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.216"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(66..2135)
                     /gene="glyS"
                     /locus_tag="ACHXOX_004100"
     CDS             complement(66..2135)
                     /gene="glyS"
                     /locus_tag="ACHXOX_004100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR7007428.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2145..3056)
                     /gene="glyQ"
                     /locus_tag="ACHXOX_004101"
     CDS             complement(2145..3056)
                     /gene="glyQ"
                     /locus_tag="ACHXOX_004101"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR7007429.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3151..3450)
                     /locus_tag="ACHXOX_004102"
     CDS             complement(3151..3450)
                     /locus_tag="ACHXOX_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR7007430.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3625..4620
                     /gene="wecH"
                     /locus_tag="ACHXOX_004103"
     CDS             3625..4620
                     /gene="wecH"
                     /locus_tag="ACHXOX_004103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR7007431.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4662..>4830)
                     /locus_tag="ACHXOX_004104"
     CDS             complement(4662..>4830)
                     /locus_tag="ACHXOX_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR7007432.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         1102 a         1270 c         1225 g         1233 t
ORIGIN      
        1 tgctgtatta tttagcgata ataaaaaaag cctgccagat ggcaggcttt ttaataacgg
       61 cgttattact gcaacagcga aatatccgca acgcgcagga acagttcgcg cagtttctcc
      121 agcatggtca gacggttgag acgcaattct ttgtcatcaa ccatgaccat cactttatcg
      181 aagaaagcat caaccggctc acgcagctct gccagttcga ccagcgcatc ctggtaacga
      241 ccttccgcaa agtacggctc cagcttgtca cgtagcacca caacctgcat cgccagttta
      301 atttcttccg gctctttcag ggtagaggca ttcacgcggt cgctcagcac ttcgtcagat
      361 ttcgccagaa tgttagatac acgtttgttc gccgccgcca gtgcagcagc tgcatccagg
      421 gtacggaaat gcgataccgc tttcatacgg gcatcgaaat cagccggacg agtcggacga
      481 cgcgccagta ccgcctggat ggtgtcaacg gtgtaacctt cgtcctgata ccaggcgcgg
      541 aagcgaccga gcataaagtc gataacatca tcaactacgt tggcgttagt cagcttatcg
      601 ccatacagac gcactgcttc ttcggtcagc gtttgcagat caaggttgag gttcttctca
      661 acgataattc gcagcacgcc aagtgcggca cgacgcagcg caaacgggtc tttgtcgcct
      721 ttcggatgct gaccgatacc gaagataccc gccagagtat ccatcttatc agcaatcgcc
      781 agcgcacaag ctaccgggtt ggacggcagg tcatcaccgg caaagcgcgg ctgatactgc
      841 tcattcagcg cgacggctac gtcttccgct tcgccatcgt gacgcgcgta gtgcatcccc
      901 ataacgcctt gggtgtcggt gaactcgaag accatgttgg tcatcaggtc acacttagac
      961 agcaggcccg cacgggttgc gtggttaacg tcagcgccaa tctgttcagc aatccagcca
     1021 gccagcgcct ggatgcggtc agttttgtca cgcagcgtac ccagctgttg ctggaacagc
     1081 acggtttgca ggcgcggcag gttatcttcc agacgttttt tacggtcggt attgaagaag
     1141 aactcggcat ccgccagacg tggacgaacg actttctcgt taccagagat aatttgctgc
     1201 ggatctttcg attcgatgtt ggcaacgaag atgaagttcg gcagcagttt gccatcgttc
     1261 gcatacaccg ggaagtattt ctggtcacct ttcatggtgt aaaccagggc ttcagccggc
     1321 accgcgagga atttctcttc gaattttgcg gtcagaacga ccggccactc caccagcgaa
     1381 gccacttctt ccagcaggct ttcgcttaag tcagcgttac cgccaatctt acgcgctgct
     1441 tcttcggcat cggctttaat cttcgcttta cgttcttcgt aatcggcgat gactttcccg
     1501 cgctcacgca gaatttccgg atactgatcg gcgttgtcga tggtgaattc cggttcgccc
     1561 ataaagcggt ggccgcgaat cacgcgatcg gactgaatgc ccagaatggt tgccggaatg
     1621 actttgtcgc ccagcagcag ggtcacggtg tgcaccggac gcacgaagtg cacgtcgctt
     1681 gcgccccaac gcatcagttt cgggatcggc agtttcgcca gcgaagtcgc aaccatattc
     1741 ggcagtagtg cttcagtgct ttcgcccttc acatgggcgc gatacagcag ccattcgcct
     1801 ttatcggtag tcagacgctc agcctggtca acggtaatac cgcaaccacg cgcccaacct
     1861 tctgccgctt tgctcggttt gccttcagcg tcgaacgcct gggcaatcgc cgggccgcgt
     1921 ttttcgattt cacgatctgg ttgcgcttcc gccaggttag ctactttcaa cgccagacga
     1981 cgcggagcag caaaccattg aacggtgccg tgtgcgaggc cagcgttatc cagctccgca
     2041 gtaaagttcg cagcaaagga ctcagccagg ctgcgcagtg cttttggtgg cagctcttca
     2101 gtgccgattt ccaccagaaa agttttctca gacatagccg cctcttactt atctttgttg
     2161 cacatcggga agccgagggc ttcacgggaa gcgtagtatg cttctgccac tgctttggtc
     2221 agggtgcgaa tgcgcagaat gtagcgctga cgctcagtga cggagatggc tttacgcgca
     2281 tccagcaggt tgaagctgtg ggcggctttc agaatacgct cgtaggctgg cagcggcagc
     2341 ggattttcca gcgccagcag ctgctgcgct tctttctcgt actgctcgaa gcaggtgaac
     2401 aggaaatcca catccgcgta ttcgaagttg taagtggact gctccacttc gttctgatgg
     2461 aacacgtcgc cgtaggtggt tttacccagc gggccgtcgc tccagaccag gtcgtaaacg
     2521 ctgtctacgc cctgaatgta catggccaga cgttccagac cgtaggtgat ctcgccggta
     2581 accggtttac actccagacc accaacctgc tggaagtaag tgaactgcgt cacttccatg
     2641 ccgtttagcc acacttccca gcccagtccc caggcaccca gagtcgggtt ttcccagtta
     2701 tcttccacga aacggatgtc gtgaatagtc gggtccatgc ccagctcttt cagagaaccg
     2761 aggtacagct cctgaatatt gtccggcgat ggcttaatga ccacctggaa ctgatagtag
     2821 tgctgtaaac ggttggggtt ttcgccgtag cgaccatcgg tcgggcgacg agaaggctga
     2881 acataagcag ccgccatcgg ttctggcccc agcgcgcgca gacaggtcat tgggtgagag
     2941 gttcccgcgc cgacttccat gtccaatggt tgaacaatgg tgcagccctg gcgagcccag
     3001 taatcctgta aggtcaggat caagccctgg aaggtcctgg tatcaaactt ttgcatatta
     3061 tttcgtgctg gatacgtgtg gatttaaagg aagggatcag tatacccgct ggatggaaga
     3121 tatacagtac gaaacgggaa aaagcagggc ttaacgcatg gaaagatgca aaaaatggcc
     3181 atctgcgtca aaagaacaga caaactgctc tttacggaag gtataaccgc gcatttcata
     3241 gcttccctgg aactgttcga aggcggtgac gtcgattttt tgctcgccgg tgttataacg
     3301 ttttgccgcc tgatccttgc ataacgcttc catattcaac gaacgcagag gatcaacctt
     3361 tacccgttgt gctttctgca cgggtgacgg tgtagaccaa cccactagca ccataagcgc
     3421 cattgccgga aagaaagcgt tcatcatcat taaattaccg tctggtttgc aggtgagtct
     3481 tattatttat atcggtatag tggctgatat cttacgaatt tccttaagcc tctataagct
     3541 gtagtgataa aactcagatt attccagtgc gccaaccaaa tgatttatcg gtcagacttt
     3601 ttttaaccag atacagagac acagatgcag cccaaaattt actggattga taacctgcga
     3661 gggatagcgt gtttaatggt ggtgatgatt cacaccacta cctggtatgt gaccaatgct
     3721 catagtgtta gccccgtcac atgggatatc gccaatgttc tgaattctgc ctctcgtgtc
     3781 agcgtgccgc tatttttcat gatttccggc tatctctttt ttggcgaacg cagcgcccag
     3841 ccgcgccatt tcttgcgtat cggcttatgt ctgatttttt atagcgcaat cgcactgctc
     3901 tacattgcgc tgtttacctc catcaatatg gagttagcgc tgaaaaacct gctgcaaaag
     3961 ccagtgtttt accacttgtg gtttttcttc gcgattgcgg tgatttatct ggtttcaccg
     4021 ctgattcagg tgaagaacgt cggcggaaaa atgttgctgg tgctaatggc ggtgattggc
     4081 attatcgcta acccaaacac agtgccgcag aaaattgacg gttttgaatg gctgccaatt
     4141 aacttatata tcaatggcga tactttttac tacattctgt atggcatgtt gggccgcgct
     4201 atagggatga tggacacaca gcataaagca ctgtcgtggg tgagcgccgc gctgtttgcg
     4261 acgggggttt ttattatctc tcgcgggaca ttatatgaat tgcagtggcg cggaaatttt
     4321 gccgatacct ggtatcttta ctgtgggccg atggttttta tctgcgcaat cgcgctattg
     4381 actctggtta aaaacacgct ggatacgcgt accattcgcg gacttggctt aatctcccgc
     4441 cattcattgg gtatatacgg attccacgcc ttgattatcc atgcgctgcg cacccgggga
     4501 attgagctta aaaattggcc aatactggat attatttgga tcttttgcgc gacgttggca
     4561 gcgagtttgt tactttctat gctggtacaa cgaatcgaca gaaacagatt agtgagttaa
     4621 gtaaaagccc gttcacattg gactgaccgg gcttacgtga attattcgct gtattcttcc
     4681 tgggtaacct gtgtttcttt tggcgggttt attccggcat cacgaatatt cttctgcacc
     4741 gctactgcac caaacaagct taagaagaaa gccagtccat aaaaaccttt ttcactgagt
     4801 aataatgtcg cattccacag acctaccatc
//
LOCUS       DAZRVY010000214         4828 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZRVY010000214 DAZRVY010000000
VERSION     DAZRVY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.217"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(103..546)
                     /locus_tag="ACHXOX_004105"
     CDS             complement(103..546)
                     /locus_tag="ACHXOX_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR7007433.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            703..1632
                     /gene="metA"
                     /locus_tag="ACHXOX_004106"
     CDS             703..1632
                     /gene="metA"
                     /locus_tag="ACHXOX_004106"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR7007434.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1901..3502
                     /gene="aceB"
                     /locus_tag="ACHXOX_004107"
                     /pseudo
     CDS             1901..3502
                     /gene="aceB"
                     /locus_tag="ACHXOX_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3532..4823
                     /gene="aceA"
                     /locus_tag="ACHXOX_004108"
                     /pseudo
     CDS             3532..4823
                     /gene="aceA"
                     /locus_tag="ACHXOX_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1203 a         1270 c         1223 g         1132 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcat tggcgcagaa aaaaatgccg ggtgcgacgc
       61 tggcgcgtct tctccagcct gggcaggcac ggcaggggca gcttattcct ccacatacgc
      121 cagattcagc aacggatacg gtttccccaa atcgtccacc tcagagcgtc ccgtaacctt
      181 aaaacccacc ttcttataga acccaaccgc ctgctcattt tgctcattaa cgttggttgt
      241 cagttccggt gccatcgaga gcgcatgctc caccagcatc cgacccacgc cgcagccgca
      301 cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca taaacccaac
      361 cggctgatcc cgttcattaa ccgcgaccca caacggcgct tccggcagga aggaacgaac
      421 tagcacctcc agctcggccc gatactctgc tgataaaaaa tcgtgtgtgg catcgacaga
      481 acgacaccaa atcgcaacga gttcctcccc ttcctcatgc cgtgagcggc gaatactaat
      541 aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc cttgaaactt
      601 tctcaccttc aacatgcagg ctcgacattg gcaaattttc tggttatctt cagctatctg
      661 gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat tcgtgtgccg
      721 gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat gacaacttct
      781 cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga tccttaacct gatgccgaag
      841 aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt gcaggtcgat
      901 attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga gcatctgaac
      961 aacttctact gtaactttga agatattcag gaacaaaact ttgacggtct gatagttacc
     1021 ggcgcgccgc tgggcctagt ggagtttaat gatgtcgctt actggccgca gatcaaacag
     1081 gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg ggcggtacag
     1141 gccgcgctca atatcctcta cggcattcct aagcaaactc gcaccgacaa actctctggc
     1201 gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt tgatgattca
     1261 ttcctggcac cgcattcgcg ctatgctgac tttccggcag cgttgattcg tgattacacc
     1321 gatctggaaa ttctggcaga gacggaagaa ggggatgcat atctgtttgc cagtaaagat
     1381 aagcgcattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct ggcgcaggaa
     1441 tatttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta tttcccgcac
     1501 aatgatccgc aaaacaaacc gcgcgcgagc tggcgtagtc acggtaattt gctgtttacc
     1561 aactggctca actattacgt ctaccagatc acgccatacg atctacggca catgaatcca
     1621 acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct tacctcaggc
     1681 accttcgggt gcctttttta tttccgaaac gcacctcagc aagtgaataa attttattca
     1741 tattgttatc aataagttat caagtatttt taattaaaat ggaaattgtt tttgattttg
     1801 cattttaaat gagtagtctt agttgtgctg aacgaaaagc gcacaacgat ccttcgttca
     1861 cagtggggaa gttttcggat ccatgacgag gagctgcacg atgactgaac aggcaacaac
     1921 aaccgatgaa ctggctttca caaggccgta tggcgagcag gagaagcaaa ttcttactgc
     1981 cgaagcggta gaatttctga ctgagctggt gacgcatttt acgccacaac gcaataaact
     2041 tctggcagcg cgcattcagc agcagcaaga tattgataac ggaacgttgc ctgattttat
     2101 ttcggaaaca gcttccattc gcgatgctga ttggaaaatt cgcgggattc ctgcggactt
     2161 agaagaccgc cgcgtagaga taactggccc ggtagagcgc aagatggtga tcaacgcgct
     2221 caacgccaat gtgaaagtct ttatggccga tttcgaagat tcactggccc cggactggaa
     2281 caaagtgatc gacgggcaaa ttaacctgcg tgatgcagtt aacggcacca tcagctatac
     2341 caatgaagca ggcaaaattt atcagctcaa gcccaatcca gcggttttga tttgtcgggt
     2401 acgcggtcta cacttgccgg aaaaacatgt cacctggcgt ggtgaggcaa tccccggtag
     2461 cctgtttgat tttgcgctct atttcttcca caactatcag gcactgttgg caaagggcag
     2521 cggtccctat ttctatctgc cgaaaaccca gtcctggcag gaagcggcct ggtggagcga
     2581 agtcttcagc tatgcagaag atcgctttaa tctgccgcgc ggcgccatca aggcgacgtt
     2641 gctgattgaa acgctgcccg ccgtgttcca gatggacgaa atccttcacg cgctgcgcga
     2701 ccatattgtt ggtctgaact gcggtcgctg ggattacatc ttcagctata tcaaaacgtt
     2761 gaaaaactat cccgatcgtg tcctgccaga cagacaggcc gtgacgatgg ataaaccatt
     2821 cctgaatgct tactcacgcc tgctgattaa aacctgccat aaacgcggtg cttttgcgat
     2881 gggcggcatg gcagcgttta ttccgagcaa agatgaagag cgtaataacc aggtgctcaa
     2941 caaagtaaaa gcggataaag cgctggaagt caataacggt cacgatggca catggatcgc
     3001 tcacccaggc cttgcggaca cggcaatggc ggtattcaac gacattctcg gctcccgtaa
     3061 aaatcagctt gaagtattgc gcgaacaaga cgcgccgatt actgccgatc agctgctggc
     3121 accttgtgac ggtgaacgca ccgaagaagg tatgcgcgcc aacattcgcg tggctgtgca
     3181 gtacatcgaa gcgtggatct ccggcaacgg ctgcgtgccg atttatggcc tgatggaaga
     3241 tgcggcgacg gctgaaattt cccgtacctc gatctggcag tggatccatc atcaaaaaac
     3301 gttgagcaat ggcaaaccgg tgaccaaagc cttgttccgc tagatgctgg gcgaagagat
     3361 gaaagtcatt gccagcgaac tgggcgaaga acgtttctcc cagggccgtt ttgacgatgc
     3421 cgcacgcttg atggaacaga tcaccacttc cgatgagtta attgatttcc tgaccctgcc
     3481 aggctaccgc ctgttagcgt aaaccaccac ataactatgg agcatctgca catgaaaacc
     3541 cgtacacaac aaattgaaga attacagaaa gagtggactc aaccgcgttg ggaaggcatt
     3601 actcgcccat acagtgcgga agatgtggtg aaattacgcg gttcagtcaa tcctgaatgc
     3661 acgctggcgc aactgggcgc agtgaaaatg tggcgtctgc tgcacggtga gtcgaaaaaa
     3721 ggctacatca acagcctcgg tgcactgact ggcggtcagg cgctgcaaca ggcgaaagcg
     3781 ggtattgaag cagtctatct gtcgggatgg caggtagcgg cggacgctaa cctggcggcc
     3841 agcatgtatc cggatcagtc gctctatccg gcaaactcgg tgccagctgt ggtggagcgg
     3901 atcaacaaca ccttccgtcg tgccgatcag atccaatggt ccgcgggcat tgagccgggc
     3961 gatccgcgct atgtcgatta cttcctgccg atcgttgccg atgcggaagc cggttttggc
     4021 ggtgtcctga atgcctttga actgatgaaa gcgatgattg aagccggtgc agcggcagtt
     4081 cacttcgaag atcagctggc gtcagtgaag aaatgcggtc acatgggcgg caaagttttg
     4141 gtgccaactc aagaagctat tcagaaactg gtcgcggcgc gtctggcagc tgacgtgacg
     4201 ggcgttccaa ccctactggt tgctcgtacc gatgccgatg cagcggatct gatcacctcc
     4261 gattgcgacc cgtatgacag cgaatttatt accggcgagc gtaccagtga aggcttcttc
     4321 cgtactcatg cgggcattga gcaagcgatc agccgtggcc tggcgtatgc gccatatgct
     4381 gacctggtct ggtgtgaaac ctccacgccg gatctggaac tggcgcgtcg ctttgcacaa
     4441 gctatccacg cgaaatatcc gggcaaactg ctggcttata actgctcgcc gtcgttcaac
     4501 tggcagaaaa acctcgacga caaaactatt gccagcttcc agcagcagct gtcggatatg
     4561 ggctacaagt tccagttcat caccctggca ggtatccaca gcatgtggtt caacatgttt
     4621 gacctggcaa acgcctatgc ccagggcgag ggtatgaagc actacgttga gaaagtgcag
     4681 cagccggaat ttgccgctat accttcgtat ctcaccagca ggaagtgggt acaggttact
     4741 tcgataaagt gacgactatt attcagggcg gcacgtcttc agtcaccgcg ctgaccggct
     4801 ccactgaaga atcgcagttc taagcaac
//
LOCUS       DAZRVY010000215         4813 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZRVY010000215 DAZRVY010000000
VERSION     DAZRVY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.218"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHXOX_004109"
     CDS             complement(34..954)
                     /locus_tag="ACHXOX_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7007435.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHXOX_004110"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHXOX_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR7007436.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHXOX_004111"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHXOX_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR7007437.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4813)
                     /gene="ynaI"
                     /locus_tag="ACHXOX_004112"
     CDS             complement(3992..>4813)
                     /gene="ynaI"
                     /locus_tag="ACHXOX_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR7007438.1"
                     /translation="KFITPDVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITS
                     ARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIM
                     LYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPG
                     RMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNI
                     MVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT         1295 a         1079 c         1137 g         1302 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa ttt
//
LOCUS       DAZRVY010000216         4799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZRVY010000216 DAZRVY010000000
VERSION     DAZRVY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.219"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..205)
                     /locus_tag="ACHXOX_004113"
     CDS             complement(<1..205)
                     /locus_tag="ACHXOX_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR7007439.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
     gene            406..1140
                     /locus_tag="ACHXOX_004114"
     CDS             406..1140
                     /locus_tag="ACHXOX_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR7007440.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="ACHXOX_004115"
     CDS             complement(1142..1753)
                     /gene="emtA"
                     /locus_tag="ACHXOX_004115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR7007441.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            1853..2767
                     /gene="ldcA"
                     /locus_tag="ACHXOX_004116"
     CDS             1853..2767
                     /gene="ldcA"
                     /locus_tag="ACHXOX_004116"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR7007442.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            2862..4598
                     /locus_tag="ACHXOX_004117"
     CDS             2862..4598
                     /locus_tag="ACHXOX_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR7007443.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
BASE COUNT         1092 a         1218 c         1240 g         1249 t
ORIGIN      
        1 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
       61 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
      121 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
      181 aaataatcca agcaataatt cccatgttca ggccctcgtt tcgatgaatc ggacgcaaga
      241 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtatgtcg
      301 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaactg
      361 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
      421 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
      481 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
      541 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgctgtg
      601 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
      661 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttatcac cgtaccgcct
      721 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
      781 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cctgtatgat
      841 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
      901 ggcatgcgct tcgctcagat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
      961 gcccagttaa tctctatcag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     1021 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     1081 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     1141 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     1201 ggtgatttcg cgctacgtgt tcgaggaact cgtcagcatc caaatcgttg attttgctga
     1261 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     1321 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     1381 ccagaatatt caggtaagcc gcccccattg aaatattacg ctccggattt ttcagctcgc
     1441 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     1501 cttttaactg catcaaacca atggcattcg atttactcac cgcattcgga ttaccacccg
     1561 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     1621 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     1681 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     1741 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     1801 cacctaagcg gcatgatata catttcgttt ctgttgtcag caaggaattg ccatgtctct
     1861 gtttcactta attgccccat cgggttactg cattaaacag cacgccgcgc tgcgtggtat
     1921 ccagcgcctt accgacgagg ggcatcaggt caataacgtc gaggttattg cccgtcgttg
     1981 tgagcgtttt gcgggtacgg aaacggagcg cctggaagat ctcaattccc ttgccagact
     2041 gaccaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     2101 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tgctcatttg
     2161 cgggcatagc gattttaccg ccattcagtg cggtcttctg gcgcatggca atgtcatcac
     2221 ctttagcggc cccatgctgg tagcaaactt tggcgcggat gaactgaacg cctttaccga
     2281 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     2341 cccgacatgt cgggccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     2401 gatcggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     2461 cgagcaccct ttccgggacg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     2521 acgtcagaag gcgatcattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     2581 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     2641 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     2701 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     2761 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     2821 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     2881 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     2941 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcaggag
     3001 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     3061 tggcactggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     3121 tggcgttagg accggcactg tcgctggcga cgctgggcgt gcttatcacc tctggtttaa
     3181 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     3241 ctatcgtcgg ctctaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     3301 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     3361 ttctgacgat taccctgatt gcgatgatcc agcaacatga aagcagtgtc agttggatgt
     3421 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggactt ggcggtggtt
     3481 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     3541 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     3601 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     3661 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     3721 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     3781 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cccttccgcg
     3841 gtttcaatct gcgtgagcgc gtgtttatca gctgggtagg attacgcggc gcggtgccaa
     3901 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcacgtctg ttctttaatg
     3961 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     4021 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc agtgtcacgc gttggtctgg
     4081 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgcc gataaatggt
     4141 gtgtaggtgc ggcactgcgc gatctgcata tgccaaaaga gacgcgtatt gcggcactat
     4201 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtgt
     4261 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
     4321 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
     4381 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataagcagc
     4441 aaacgctggg tgaaattgtc cagcagttgt taggcgcggc accggttgtc ggtgaccagg
     4501 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
     4561 ttggcgtacg ggtggctgaa gaggaagctg aatcttaaat ttaatgaagc atctatcgcc
     4621 ggttgcgatg ctttgctgaa cgcagcctga gcctgcaatt gtttgtcgtc cataatttaa
     4681 ccttcatttg atgttgaatt gaacatatca aaatcaggca attacacaaa tctatgtaca
     4741 ggctccatca agggtaaagc gtgatttatc tgaagtcgag ttcgagtcaa caatgaagc
//
LOCUS       DAZRVY010000217         4798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZRVY010000217 DAZRVY010000000
VERSION     DAZRVY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.220"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..76
                     /locus_tag="ACHXOX_004118"
     tRNA            1..76
                     /locus_tag="ACHXOX_004118"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Thr,seq:ggt)
     gene            116..231
                     /gene="rrf"
                     /locus_tag="ACHXOX_004119"
     rRNA            116..231
                     /gene="rrf"
                     /locus_tag="ACHXOX_004119"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(294..1052)
                     /locus_tag="ACHXOX_004120"
     CDS             complement(294..1052)
                     /locus_tag="ACHXOX_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR7007444.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1060..2163)
                     /locus_tag="ACHXOX_004121"
     CDS             complement(1060..2163)
                     /locus_tag="ACHXOX_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR7007445.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2173..3354)
                     /locus_tag="ACHXOX_004122"
                     /pseudo
     CDS             complement(2173..3354)
                     /locus_tag="ACHXOX_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3422..4454)
                     /locus_tag="ACHXOX_004123"
                     /pseudo
     CDS             complement(3422..4454)
                     /locus_tag="ACHXOX_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1316 a         1240 c         1145 g         1097 t
ORIGIN      
        1 gctgatatgg ctcagttggt agagcgcacc cttggtaagg gtgaggtccc cagttcgact
       61 ctgggtatca gcaccacttt ttaggttaaa gttcggcaga ttagaaaaga atttgtctgg
      121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
      181 cgccgatggt agtgtagggt ctccccatgc gagagtaggg aactgccaga catcaaatca
      241 agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt caattaatgg
      301 attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa aaattcatca
      361 ggtgcagctt gctccactat ttccccacga tccataaaaa tcacccggtc agcgacggtt
      421 cgcgcaaacc ccatctcatg cgttacacac aacattgtca tacccgattg cgccagccca
      481 atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt tggctcatca
      541 aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg ttgctgctga
      601 ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac ccgctcaagg
      661 taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca aatcggtgcc
      721 agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg aaagaccatt
      781 cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc gatgccatct
      841 actacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat ggttgtcgat
      901 ttaccggaac cagaagggcc acacagaacg atccgttctc ccagttgcac ggttaaattt
      961 atatttttca aaacatggaa ttgtccatac catttattga cgttttccag cgtaatcatc
     1021 gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg tacgcccggt
     1081 gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga agatccagta
     1141 gatcagtgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg gatcaacggt
     1201 tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca ggctggtatc
     1261 tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca acgcctgtgg
     1321 cagaataacc agcccctgag ttttccagta acccaatgcc agtgactctg ccgcttcata
     1381 ttgcccttta ggcagcgcct gtaatccgcc tcgcacgact tccgcaacat atgctgactg
     1441 aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atattggtgc cttctgccat
     1501 aaacaaaggc agcatgaccg aagacataaa caatacggta atcagcggta cgccgcgcca
     1561 gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc ggcgacctaa
     1621 cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa taattaaagt
     1681 tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa aaccgccata
     1741 catcagccac cagacaatca gtgggtaaat caccgcccag accgcaatat agcgaccgcg
     1801 atgcgggagt attttccaga acattggtgc gattgacaca aggccaatca gtaatgccag
     1861 gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac caaatcgttc
     1921 gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg agccaaccca
     1981 attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca tcaaccatat
     2041 gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc gcacccacac
     2101 catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca atacttttgt
     2161 catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat tcatcagcag
     2221 cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg tctcgatggc
     2281 ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg gatagccaat
     2341 agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta acggcggaat
     2401 aatcactcgc agtgcctggg gaataatgac ctagcgtagc gtaaccgggt tgggtaatcc
     2461 cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag cgcggataat
     2521 ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg ccagttcagg
     2581 aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat cccagtgtag
     2641 tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca ctgcggcgat
     2701 cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa agcggaatag
     2761 cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa atccgtctcc
     2821 cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat caaacgcgct
     2881 cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga agaagatttg
     2941 cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct ttcgtagcag
     3001 ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag cgaacacaat
     3061 acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc gtccgtaggt
     3121 gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc cgcgatccag
     3181 aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt tgtgaaataa
     3241 ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc aggcacgaac
     3301 cgtagggttg gcaaaagaga gtgagccttt aacggttgag cggcgatgag acatagaaac
     3361 ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac ctcacaccga
     3421 cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta ttttgcccac
     3481 gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc gagtagttac
     3541 ccacctgttt gatgatgtta taggcccatt tattatccag cttcagatcc ttgccgtaat
     3601 cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc gctttttcat
     3661 cgacgttctg ggaattgatg cccatctctt cagcattcag cattcagcat ggcgaaaagc
     3721 gtccagcgta caatcgagaa ccattcatca tcgccacgac gaactaccgg cccaagcggt
     3781 tctttagaga taacctccgg taagacaatc cattcagctg ggttgcttaa tttgatgcgc
     3841 agggcataca gttgcgactg atccgaggcc agcgtatcgc agcgaccaga ttccagtgcc
     3901 ttcgctgatt cgtcagagcg atcgaaagtc accggtgtgt acttcatatt gtttgccttg
     3961 aaatagtcgg cgacgttgag ttcggtatct gtacccgcct gaatacagac ggtagcgcca
     4021 tccagttctt tcgcgctttt tagccccgct ttatcgtgcg tcaggaagcc aatgccgtcg
     4081 taatcagtga cgccagtaaa tgccattccc atcccggcat cgcgagatga agtccaggtc
     4141 gtattacggg agagcaaatc cacctcccct gactgtaaag cggtgaagcg ttcttttgca
     4201 gtgagcgggg tatatttcac tttcgtgtcg tcaccaaata cagcagcggc aacaccacga
     4261 caaacatcta catcaatacc tgaaaactta ccgtctgcat cggcataaga gaacccaggt
     4321 aatccatcac tgatcccgca ttgcacaaaa ccttttttct gtacggcatc aagcgtcgcg
     4381 ccagcatgcg cctgatttgc aacggcaagc agcacgctgg cggcagccag tgtggctatc
     4441 atcatctttt tcataatgct tcctgtgagg cgaaaattat cgttatagga cgtttgggaa
     4501 acgtctttta cctgtctgtg gccatcctct tataagcaaa agtagtgcca tcttttgcgc
     4561 acgatttcgc gtcgcaataa gctgagtgta gacaggaaat aataaaaaca gcgccccgca
     4621 atggtgcgaa aatgaaacat gcgccacatt ttggcgcaaa atatttacgc gtgttttact
     4681 aagcagaata atgattgtta gcgtgagtat atttctggtg ggaggtgtga aagttaaatt
     4741 gatgcgttat ttcgaaagca gcgaaataac gcgatataac atgctaaggt aatgactc
//
LOCUS       DAZRVY010000218         4772 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZRVY010000218 DAZRVY010000000
VERSION     DAZRVY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.221"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            27..689
                     /locus_tag="ACHXOX_004124"
     CDS             27..689
                     /locus_tag="ACHXOX_004124"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR7007446.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1053..1939
                     /locus_tag="ACHXOX_004125"
                     /pseudo
     CDS             1053..1939
                     /locus_tag="ACHXOX_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1939..2228
                     /locus_tag="ACHXOX_004126"
                     /pseudo
     CDS             1939..2228
                     /locus_tag="ACHXOX_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2549..2839
                     /locus_tag="ACHXOX_004127"
     CDS             2549..2839
                     /locus_tag="ACHXOX_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007447.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2839..3258
                     /locus_tag="ACHXOX_004128"
     CDS             2839..3258
                     /locus_tag="ACHXOX_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007448.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            3426..3749
                     /locus_tag="ACHXOX_004129"
     CDS             3426..3749
                     /locus_tag="ACHXOX_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007449.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4179..>4772
                     /locus_tag="ACHXOX_004130"
     CDS             4179..>4772
                     /locus_tag="ACHXOX_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007450.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1562 a          879 c          978 g         1353 t
ORIGIN      
        1 gattgcaata aaaattttta tttcttatgt caattgagca tattttaatc gggaagcatc
       61 atgggggttc tgattactgg agaactccat ttttgttatt taagaactta caccgtgaat
      121 ttattttcag tctagatggt gctgcaactg agcatgacac gctgttacca cgattcaccg
      181 atgatataag ccgtcaatca tgggttagtg aaaaggtctt ctgtaacccg ccgtacagcg
      241 atattccgtc gttcctctta aaagcgtctg aagcggattt agtagctttc ctgataccgc
      301 atagagccaa caccagctac tggcttaggc acatttacag caataaccac tgtcatgaaa
      361 tccgtattct tcatcgtgca gttaaatacc ttccaccagc agggcataac aggcttacta
      421 ttaggtcacc gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt aagcatgaaa
      481 ttacacagat ggtttgctgt gctgatacat tgctaccgtt aaccatcatt aaccgtggtg
      541 gactaagagg aagacccacc atttacccac ctgagacgct cgacagcttc ataaaactat
      601 acaggcaagg aaaacccatt aagtacattg cagatgctct cagaatgcct ctgagcacgt
      661 catacaggat tgctcaaagg ttgagctaaa attctcataa atatggtcat ttacaagaca
      721 ggtgattcag atgttaagaa gtatccctgt gaacaataaa tgaccaatca gtaatcattc
      781 tgtgaaataa ttgttgcaat ttataatcac atctgttata gtttctatat gggctgataa
      841 aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt cagatggttg gcgtatatta
      901 acctctcatt cagggtatac ctcagcgttt taatcatcgt actgtttcag gcatggactt
      961 ctttatcagt ccatcaatcc cccagatatt ttaatcacac aggctaatcg gttttatccg
     1021 gttggccttt tcacgtatga ggtaatttat gaatgtctaa atataaccca aatcagtttt
     1081 ataaatttga tgtgcgaatc tctcaacttt cagaagaaat taacgcagca gcaagattga
     1141 tttatatggt catggtccat agctgtgact tctatgaaga gaaatacaaa tcatattcac
     1201 caagtattga ccatctcgct tatatggctg gtgtatcaaa gagaacagca atcagagcca
     1261 ttgagacact tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg cgtcaaagca
     1321 cttatcgtgt attcacactg gatgataagc cggaattact tgtaagacct acatcacagg
     1381 aagttgcagc ctcagtaaga caaaatgaca ccactaccag tgacaaaatg tcatgggggt
     1441 agtgacaaaa tgtcacccgt gttagagaga taagttatta gataaaacat tagataataa
     1501 tattacagat aagaataaag agactggact taatgatgat attaaagaag atgactttct
     1561 ttctttggat gaattaaatg attttctctc tatgaatgaa tctgaccttg aagtaagctc
     1621 taaaaagact tctaaacaag acagagaaat cactgatgtg agtgttgata gtgaccataa
     1681 aaacacacag tgccccgcca ctgatgatga gttggacccc ttcgatgatg aagaggatga
     1741 acgtgaaaaa gccattaaga gggcagagaa gaaaaagaga ttcgagcttg caagacaaaa
     1801 gaacaaagta tcgggtttag acccattcga tgacagtgaa gatgattatt atctttcacc
     1861 gaaaaagaaa caaactcagg atgacaagaa gcaaaaaatt aaaccgaaga tgtattttgc
     1921 cggtggagga attaagtaat gaaagaatat cacggtgaaa agagatacaa agattacctg
     1981 ttaaagcgtt acagcatttc tcgtgaaggt catcttatga aggatactca cgggaagttt
     2041 accggattag accaaagaaa gaagggaggg gttatttctt ttacgatggt gtgacagatt
     2101 taaagattga cgctttgagg tttgcagtgc tgtatcattt tgatatctgg aattcaatcc
     2161 accagctacg attaaaagac ggtgacccca gcaacttaaa ggccaccaat attatcacaa
     2221 aacgttagtt tgttttacca tcatcaatcg taattgtgat ttcatatttg aaaagcttaa
     2281 tgctgaaatt gaatcgtgta cttctggcga taatcatttt taaaagctga tacacaagaa
     2341 gcaccagaaa cacaaagcaa cctaagcttg cgaatctgtg agattcagca attgtttcaa
     2401 gaaagcccat taattcccct tatttatcaa agggaatgac ctgtatcagc cccctacaac
     2461 catattaaca tgtaaatgag ttgttgacaa tatgttataa atatgttaaa gttagttata
     2521 agtcaaagat tttaagaagg aaaatttaat gagacacaca gttacacgcc ttttgccaga
     2581 ggcatattcc agactggcag caatcaaaga caaataccca gccatgagcc acacagcact
     2641 tgttggtgag attctgaagg aacacacagc ccttacaccg tttgacgcta tcaagggcgt
     2701 tgcagagggt tctaaggcca tcagaatcac aacgggccaa ctggaactac tcaaagcaca
     2761 gtccgcagtg gcaggttgct caatgattca ggtgttgagt gccaaggttc atcaactggc
     2821 ggataagctg ggggtttaat gctatgcaca tttgattatc atgcgtttta tcgccataga
     2881 ggtcaccttg tccgtgagaa taacggaaca tggaaagatt taactcgtaa tgctgaaccc
     2941 ttcacacacc agcaattaaa atcccgcttc tccagatgga agaatgcacc agcagatact
     3001 ggtgattttg agattatcca taccttcgat tatgcctcag aagatgacct tcacgatgca
     3061 attaagctga ttgaagactt caatacctca gaagatgtgg atggtgacaa atataaatcc
     3121 cgtggtaacc gctataaccg tgatgatatc actcaggcca taaatgctgg tcaatctcag
     3181 cgatacatta cagaaaaata tggtatttca cggagacagt atttctacgt taagtcattg
     3241 attaataaag acaattaaaa ataaccatct aagaaacggt agaatgcact ttacaggcag
     3301 agttgcacct taatttgata aattacctta ccagccagtg caactccctc tgtaacccgc
     3361 atggttactg gcctcagaat ggctatttta agaggtctga ggtgtgtttc ataggagatt
     3421 taaaaatgaa taattaccca atgcaaatta tggttgataa cgacactgct atgatggtgc
     3481 aaagcttcat tgatgcaggt atcgcaatta attttgataa attactaaag ctgatggctg
     3541 ataatgcaga aaacatctct gactttattc agtcggtgga gtttaacgag cctcgaatga
     3601 tgctaccaat tacagacagc aacatgaaaa gattggttat cgagcaaacg aacatataca
     3661 gtatttcccc agagcaattt ttgaaaggtg ctgtgacaat cctgtacagt gataatattc
     3721 tggtgacgga ttctgtgagg gttcactaat gggtttaata attgcttgga cgttgcttgg
     3781 cttatttgtt ggttgcatta ttaacaatgc ggtaaataag aattgatgtt atcaacctgt
     3841 taaaaagctg ttgacaaata accttaagca tggcataatt ttaattatgg gagagcaatt
     3901 ctctcttatt tttctttcaa taatccgatg gatggatatt ctgagttgga aaggtgcaat
     3961 gcttttcgtc atggcctttc aggttagtct gagaggctat ctcaaaaagt actcttgttc
     4021 attcattatt gaatcctttg cccttcacag aaatgtttag ggctttttca gtttttaaaa
     4081 gcacacttgg caggttcttg ttcagtcaga ctcttcacct ctgattgctg agtgtgcttc
     4141 tataaattga aataacaaat aaactaaggg gtgaacgaat gattagaaat gaacatgaaa
     4201 tgcttatcga acacagacca agtgcacttg atattcacga attaactttg cgtgaatctt
     4261 ttgatagagc ttggcgggaa tcacacgaag taaataaagg tccatatatt ccaccagaac
     4321 cactcgagat tccaagagtt gaaattgatt tttctatgaa cgaacggtgt gaacttgatt
     4381 tgaagttgaa gctacaacgc cgatatttcc aagctattaa agactctcaa gaagcaatga
     4441 gaagtgttta cgagaaaatt cagtccaagc ttgaagacga aattgacgaa atggttattt
     4501 cgatggagct tcaaaccaat tcttatgctt gtttctctcg actagaccgt gacggttggg
     4561 ggtatgaccc tgtagagatt ggtaaagtga ttgcagattt accagaaggt caaagagttg
     4621 ttcgtacaga aagagctgtc agagcaccac atcgactgtc tctaattgtt gctgataaat
     4681 cacaagctga gcttgaagaa attgccaaag agaaactgat gaaagcaaga gaaaatgaga
     4741 ttaatttgct gaaatcaaga ctggctaagc tg
//
LOCUS       DAZRVY010000219         4701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZRVY010000219 DAZRVY010000000
VERSION     DAZRVY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.222"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            387..1469
                     /locus_tag="ACHXOX_004131"
     CDS             387..1469
                     /locus_tag="ACHXOX_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR7007451.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHXOX_004132"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHXOX_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR7007452.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="ACHXOX_004133"
     CDS             complement(2127..2267)
                     /locus_tag="ACHXOX_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR7007453.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHXOX_004134"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHXOX_004134"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR7007454.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="ACHXOX_004135"
     CDS             complement(2623..2913)
                     /locus_tag="ACHXOX_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR7007455.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="ACHXOX_004136"
     CDS             complement(2906..3076)
                     /locus_tag="ACHXOX_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR7007456.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="ACHXOX_004137"
     CDS             complement(3076..3531)
                     /locus_tag="ACHXOX_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR7007457.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="ACHXOX_004138"
     CDS             3796..4173
                     /locus_tag="ACHXOX_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007458.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            4170..4598
                     /locus_tag="ACHXOX_004139"
     CDS             4170..4598
                     /locus_tag="ACHXOX_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007459.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1350 a         1049 c          980 g         1322 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatggcactc
     4201 aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga aaaacggtgt
     4261 ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg attttcattc
     4321 cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca ttatttcctg
     4381 aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata ttaagtgcaa
     4441 tagtagcata ttttgttttc cataagcata aatctgccac tctatccata tgcagcttag
     4501 tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc gacctaacac
     4561 aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat gcgaagtatg
     4621 catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc taaatattat
     4681 tctttattat cttctttacc a
//
LOCUS       DAZRVY010000220         4641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZRVY010000220 DAZRVY010000000
VERSION     DAZRVY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.223"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(12..2108)
                     /locus_tag="ACHXOX_004140"
     CDS             complement(12..2108)
                     /locus_tag="ACHXOX_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR7007460.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(2108..2854)
                     /locus_tag="ACHXOX_004141"
     CDS             complement(2108..2854)
                     /locus_tag="ACHXOX_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR7007461.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(2851..3495)
                     /gene="gfcB"
                     /locus_tag="ACHXOX_004142"
     CDS             complement(2851..3495)
                     /gene="gfcB"
                     /locus_tag="ACHXOX_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR7007462.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(3602..3907)
                     /locus_tag="ACHXOX_004143"
     CDS             complement(3602..3907)
                     /locus_tag="ACHXOX_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007463.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(4354..4566)
                     /gene="cspH"
                     /locus_tag="ACHXOX_004144"
     CDS             complement(4354..4566)
                     /gene="cspH"
                     /locus_tag="ACHXOX_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR7007464.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         1183 a         1149 c         1140 g         1169 t
ORIGIN      
        1 attacgtcag gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg
       61 tatttgcgtg acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg
      121 cggttgcggt taggtccaat ggtcatcaag tcgaacggaa tcgagatata aaaaccttta
      181 ctaaagccgc cttcgccgta gtcatcttta gatacgttac tgattgccgc ccatacccct
      241 accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg ttgccccttt atcttttgcc
      301 agatattgcc caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa
      361 gccgtcacga aaccagttgg cgtggaataa tcggtgaagc gcatcatgtt gtcccagtca
      421 cgttgcttca cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa
      481 agcagctcgg aaccgacacc ggcgtacatc gtttccagat aaccgccata cacctggcca
      541 tagaaaccat tgcctaagtc ggcaaagtag ttcgcctgca agttgttgag ataaacgtca
      601 ttgcgaacgt aatcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggcaac
      661 tgcgaagact taaacttgtc gtagttgttg taaatattgg tgaaaatacc gccatcaagc
      721 agcaggtggt cggtaaacca gtagcgggca ctggacatca gccccagctg gaacatatag
      781 aaatcttccg ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga
      841 tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat cttctgcttc aacacgttgt
      901 tgttgaagtt gctctgattg accaggcgct gtaccagcca gctgtttgcg caggctggct
      961 acgtcggttt cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc
     1021 tcaacgcctt gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcgcga
     1081 gaatcacggt atttgtattg ttgaccagac atatacagcg tcttatcgcg cagctgaatt
     1141 tccggtgctt caaaacctgc gttgtacttc agcgcggtaa gttggttcgc tactgtggtg
     1201 tactgcaatc cttcagattc aggcgcaggt tgatatgccg gttttggcgt atcgcgcagg
     1261 gcagggcgca gatcgttgaa attggtccgt aacgtgaagc caaacatcaa cgtgttaccg
     1321 cgttcataac tcaggttgag atctgcccag ctggcagcgc gataaactgc gccgacgttg
     1381 aaatggcttg cctgaggcag tttgccagcg aaatcattct ggtaattgtt gccatcgtat
     1441 tcgagtttca gacgcagggg attccacggc gtttgatact caatgccgcc aaagatggaa
     1501 gccgggccac gaaagatatc gctaaagctg atatcgcccg catcgtgaga ctctgcgcga
     1561 tgacaatatt tatcgctcac ccggcaaaac gggttggtaa tattgcccgc attaccggcg
     1621 tagccccatg ccatcccgag ggtgaaatca aatggccccg cttgcttgct ggccaccaga
     1681 tactcaccgt caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt
     1741 agccagtaac cttcttccca caggcgaagt ttaaaatcga atgatttgtc tttgtatgac
     1801 tgatcgccgc tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc cgtataacga
     1861 atggtgcctt ccagccatgg gaacagcgcc acggaggtgg agtagaaccg gtattgatcg
     1921 ttatcccggt agttgacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc
     1981 aacaatcctg tgccgccaaa gtctgattgc gacgaaccca gcggatccgg gtaggttaat
     2041 acttcagcat gacaggcact ggagacggca atggccaggc agcttaaaag ataagaattc
     2101 ttcttcatca gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag
     2161 catatttttc gggcagtaca tgcgcagaaa agcccaacca aagctggctg ccaggcggtg
     2221 gttccacatg tcttttattc cacagggcaa caggggcgac gacagtctca ccctcaggcg
     2281 taatcaccat aacgttattc ttgtccgcgc ctgcaaggcg ggggtgatct tgcagataat
     2341 cggtaacgct acggcctgcc tgccagggca attgcccggc cccagaaacc gcacccagca
     2401 gagtaatagt caccggacgc tgtacggtat agagcgtata gtccccgacc aacgggggat
     2461 tgctgttctc atccactcgt acaaaatcag ggtccagttt gaccggtaag cgaccagtga
     2521 tattgagatt gagtaactgc tggcgcacgg atttaatcgt tgccgccaca tcgtcgtcag
     2581 cttctgcttc ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag
     2641 ctttcgccgc agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg
     2701 gttgtgtcac caactggaca acattttcca tggggccaac ggaaagtgtc tgttgttcgc
     2761 caggcagata aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta
     2821 cgctggcaat gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc
     2881 tacgggaata acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga
     2941 atcgacccag aatgtgtttt gccactgttt ttctggccgc gtggaagtca cctcttcctg
     3001 ccagacatta cacagcacat tgcgacccgc aatattcagc gtctctaatc cagcaaagga
     3061 aaaagtagaa ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg
     3121 cgtccagcgg ctgccatctt ttattgccag cgcgttacgc agagggtcct gcccggaatt
     3181 tgtcacttcc agcagattat tattaagttt gacggttttt aaaagacgtc cattgtgggt
     3241 aaccagcatg gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag
     3301 taccacaaag attcgttgcc cttcattaag gcgtaaatac atcgtggaat aaggcaacgc
     3361 ctggatctgc tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt
     3421 atcgaccata ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa
     3481 aataagaggg cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctctaaag
     3541 cccggtacga atatcgcaac cgggtatcat ttaactttaa tttcgacgcg ttaaagcagt
     3601 attagcgtgt agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat
     3661 ttgctgcaac aactgccaac gtcgccacac caacagccgt tgccacaccg acaactgtcg
     3721 ccgtgccgac agcgccagca gccacgccac cagtggtagt agcagtcgtg gttgcgccgg
     3781 tagtggtcgt tgtggtgcca gtagtacccg tcgttgcctc ggtcccgttt gttgcttcag
     3841 aggcagcaaa cgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt
     3901 gtttcataac ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggattg
     3961 tctactttga gtataacatc cttgtgaaat ctccctttcc tcataccgct tgcactatat
     4021 actgatactg cgggtaggaa aggtgcgcag agggaaatat aagattatta acgatcccct
     4081 ctggaaatac gacttgtgag cataagataa atcttagcaa aatacaaatt aaagataagg
     4141 aaattgttac gaaagctatt aattattgtt ggtaatatag tttcaagtgg tactataact
     4201 ggctgttaaa tggcatttat aactattagg tgcagagata ttcgcttaaa tggattagtt
     4261 tctttgtgga aaagtaactg atgttataat gtatgatgtt gactattgca ctattccaat
     4321 aaaaccagtc agctttaaac aagcagcgtc atattaagag agataaacat ttgccgctgt
     4381 tggtcctcgc aggccattta cgcggcaaaa ttccacacgt aatcctggta taagcacttc
     4441 tgcgtcgcgg ggagtgaatg cggaaatatg gacctgaact tctttacgac cgtcggaggg
     4501 gataatgaat cctttgccgc ttttgcgatc aaaggttttg acaattcctg tcattttacg
     4561 ggacaaacaa attccttact gaaaatactg cgctgcacta tacggggtta ataaaataaa
     4621 gccagcgata tttaagaccg c
//
LOCUS       DAZRVY010000221         4503 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZRVY010000221 DAZRVY010000000
VERSION     DAZRVY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.224"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHXOX_004145"
     CDS             <1..461
                     /locus_tag="ACHXOX_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR7007465.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHXOX_004146"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHXOX_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR7007466.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..4492)
                     /locus_tag="ACHXOX_004147"
     CDS             complement(2231..4492)
                     /locus_tag="ACHXOX_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR7007467.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1144 a         1052 c         1047 g         1260 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc ataaatttcc
     4501 ttg
//
LOCUS       DAZRVY010000222         4482 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZRVY010000222 DAZRVY010000000
VERSION     DAZRVY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.225"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHXOX_004148"
     CDS             complement(7..1068)
                     /locus_tag="ACHXOX_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR7007468.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHXOX_004149"
     CDS             complement(1065..2462)
                     /locus_tag="ACHXOX_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR7007469.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHXOX_004150"
     CDS             2617..3615
                     /locus_tag="ACHXOX_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7007470.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHXOX_004151"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHXOX_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR7007471.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1099 g         1062 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaatggattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZRVY010000223         4409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZRVY010000223 DAZRVY010000000
VERSION     DAZRVY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.226"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHXOX_004152"
     CDS             complement(<1..105)
                     /locus_tag="ACHXOX_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007472.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHXOX_004153"
     CDS             complement(173..2122)
                     /locus_tag="ACHXOX_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7007473.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHXOX_004154"
     CDS             complement(2207..2542)
                     /locus_tag="ACHXOX_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR7007474.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHXOX_004155"
     CDS             complement(2542..2898)
                     /locus_tag="ACHXOX_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR7007475.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHXOX_004156"
     CDS             complement(2898..4394)
                     /locus_tag="ACHXOX_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR7007476.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZRVY010000224         4395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZRVY010000224 DAZRVY010000000
VERSION     DAZRVY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.227"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHXOX_004157"
     CDS             complement(<1..590)
                     /locus_tag="ACHXOX_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR7007477.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHXOX_004158"
     CDS             complement(583..993)
                     /locus_tag="ACHXOX_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR7007478.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHXOX_004159"
     CDS             complement(999..1532)
                     /locus_tag="ACHXOX_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR7007479.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHXOX_004160"
     CDS             complement(1510..2586)
                     /locus_tag="ACHXOX_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR7007480.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="ACHXOX_004161"
     CDS             complement(2583..3974)
                     /locus_tag="ACHXOX_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR7007481.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..>4395)
                     /locus_tag="ACHXOX_004162"
     CDS             complement(4021..>4395)
                     /locus_tag="ACHXOX_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007482.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1023 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgt
//
LOCUS       DAZRVY010000225         4364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZRVY010000225 DAZRVY010000000
VERSION     DAZRVY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.228"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXOX_004163"
     CDS             complement(<1..215)
                     /locus_tag="ACHXOX_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7007483.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHXOX_004164"
     tRNA            complement(330..405)
                     /locus_tag="ACHXOX_004164"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHXOX_004165"
     tRNA            complement(412..486)
                     /locus_tag="ACHXOX_004165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHXOX_004166"
     tRNA            complement(603..687)
                     /locus_tag="ACHXOX_004166"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHXOX_004167"
     tRNA            complement(696..771)
                     /locus_tag="ACHXOX_004167"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXOX_004168"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXOX_004168"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR7007484.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXOX_004169"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXOX_004169"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR7007485.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXOX_004170"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXOX_004170"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR7007486.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHXOX_004171"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHXOX_004171"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZRVY010000226         4349 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZRVY010000226 DAZRVY010000000
VERSION     DAZRVY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.229"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHXOX_004172"
     CDS             73..732
                     /locus_tag="ACHXOX_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR7007487.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHXOX_004173"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHXOX_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR7007488.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHXOX_004174"
     CDS             complement(1860..2570)
                     /locus_tag="ACHXOX_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR7007489.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHXOX_004175"
     CDS             3004..3294
                     /locus_tag="ACHXOX_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR7007490.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHXOX_004176"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHXOX_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR7007491.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHXOX_004177"
     CDS             3927..3998
                     /locus_tag="ACHXOX_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007492.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHXOX_004178"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHXOX_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR7007493.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
BASE COUNT         1169 a         1058 c         1037 g         1085 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatat
//
LOCUS       DAZRVY010000227         4329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZRVY010000227 DAZRVY010000000
VERSION     DAZRVY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.230"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..582)
                     /locus_tag="ACHXOX_004179"
     CDS             complement(<1..582)
                     /locus_tag="ACHXOX_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7007494.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(645..1094)
                     /locus_tag="ACHXOX_004180"
     CDS             complement(645..1094)
                     /locus_tag="ACHXOX_004180"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR7007495.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHXOX_004181"
                     /pseudo
     CDS             complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHXOX_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1043 t
ORIGIN      
        1 gaagttcggg atcatcgcgt caacggtgat ggcgtcagca ggctgtacgc cgccaaccag
       61 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
      121 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
      181 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt atccgaggaa
      241 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
      301 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
      361 tgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
      421 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
      481 atcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
      541 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
      601 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
      661 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
      721 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
      781 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
      841 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
      901 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
      961 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
     1021 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
     1081 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
     1141 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
     1201 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
     1261 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
     1321 gcagctcgtt acagtgctgg gcagcgtgga tgttttccta ggtatagtgc caggcgctga
     1381 agttaattgg gcgctgcgga accaccagca gaccgttacc gtggcggtta gtcagtgccg
     1441 tccagcggac atgctgacgg ttaccgttgt tctgcgggaa gggatagttc tcgaacatgg
     1501 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
     1561 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatt cccatggtga
     1621 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
     1681 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcattccg aaatcaaaca
     1741 ccggcggggc aataaccgtg cggctgatga tcaacacttc accctcgctc tgttctacgg
     1801 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
     1861 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
     1921 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
     1981 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
     2041 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
     2101 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
     2161 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
     2221 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
     2281 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
     2341 tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac cggcgcgata acctgtttgt
     2401 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
     2461 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgtggt
     2521 cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc tgaatgcaat
     2581 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
     2641 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
     2701 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
     2761 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
     2821 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
     2881 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
     2941 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
     3001 tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
     3061 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
     3121 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
     3181 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
     3241 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
     3301 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
     3361 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
     3421 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
     3481 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacgg
     3541 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
     3601 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
     3661 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
     3721 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
     3781 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
     3841 gttgataggc accggttggg ttatcgctgg gaacaaacgg cacatcgatg gggaacggaa
     3901 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
     3961 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcgggt
     4021 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc
     4081 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
     4141 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
     4201 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
     4261 tttttactaa aaagtggcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
     4321 cagcactgt
//
LOCUS       DAZRVY010000228         4309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZRVY010000228 DAZRVY010000000
VERSION     DAZRVY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.231"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHXOX_004182"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHXOX_004182"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR7007496.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHXOX_004183"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHXOX_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR7007497.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHXOX_004184"
     CDS             2269..2976
                     /locus_tag="ACHXOX_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR7007498.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHXOX_004185"
     tRNA            3082..3157
                     /locus_tag="ACHXOX_004185"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHXOX_004186"
     CDS             3355..>4309
                     /locus_tag="ACHXOX_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7007499.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZRVY010000229         4261 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZRVY010000229 DAZRVY010000000
VERSION     DAZRVY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.232"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHXOX_004187"
     CDS             complement(<1..3511)
                     /locus_tag="ACHXOX_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR7007500.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4261)
                     /locus_tag="ACHXOX_004188"
     CDS             complement(3963..>4261)
                     /locus_tag="ACHXOX_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7007501.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1098 a         1095 c          878 g         1190 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 g
//
LOCUS       DAZRVY010000230         4198 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZRVY010000230 DAZRVY010000000
VERSION     DAZRVY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.233"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHXOX_004189"
     CDS             complement(<1..1361)
                     /locus_tag="ACHXOX_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR7007502.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHXOX_004190"
     CDS             complement(1426..1545)
                     /locus_tag="ACHXOX_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR7007503.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHXOX_004191"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHXOX_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..3750)
                     /locus_tag="ACHXOX_004192"
     CDS             complement(2272..3750)
                     /locus_tag="ACHXOX_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR7007504.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1185 a          920 c          738 g         1355 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtg
//
LOCUS       DAZRVY010000231         4180 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZRVY010000231 DAZRVY010000000
VERSION     DAZRVY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.234"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHXOX_004193"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHXOX_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR7007505.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHXOX_004194"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHXOX_004194"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR7007506.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHXOX_004195"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHXOX_004195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR7007507.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4087..>4180
                     /locus_tag="ACHXOX_004196"
     CDS             4087..>4180
                     /locus_tag="ACHXOX_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007508.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLS"
BASE COUNT         1030 a         1062 c          966 g         1122 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcaggt
     3841 catttcgtaa ttacattaat taaaaaactt aaaaccatta aataaataaa aaaccactaa
     3901 ctctatgtga aataaatcaa aatttcacgc tgcaatactc cttagaatgt atagcgaaag
     3961 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcaa
     4021 cttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4081 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4141 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg
//
LOCUS       DAZRVY010000232         4088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZRVY010000232 DAZRVY010000000
VERSION     DAZRVY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.235"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            62..670
                     /locus_tag="ACHXOX_004197"
     CDS             62..670
                     /locus_tag="ACHXOX_004197"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR7007509.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            768..2159
                     /gene="selA"
                     /locus_tag="ACHXOX_004198"
     CDS             768..2159
                     /gene="selA"
                     /locus_tag="ACHXOX_004198"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR7007510.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2156..4000
                     /gene="selB"
                     /locus_tag="ACHXOX_004199"
     CDS             2156..4000
                     /gene="selB"
                     /locus_tag="ACHXOX_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR7007511.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          913 a         1033 c         1211 g          931 t
ORIGIN      
        1 ttctgccctt tttcccatca ccaggccata ctgatatttt tcctcgcaca aaggactcgg
       61 catgaaactc gtcggtagct acaccagccc gtttgtacgc aaactttcta ttctgttgtt
      121 agaaaagggc ataactttcg aatttattaa tgaactgccc tataacgcgg acaacggcgt
      181 ggcgcaattt aacccgttag gaaaagtgcc ggtgctggtg accgaagagg gcgaatgctg
      241 gtttgattcg ccgatcatcg ctgaatatat tgagttaatg aatgtcgctc cggcgatgtt
      301 gccgcgcgat ccgctggagt cgttgcgggt gcgcaaaatt gaggcgctgg cggatggcat
      361 tatggatgcc gggctggtat cggtgcgtga acaggcgcgt ccggcggcgc agcagtctga
      421 agatgaattg ttacgccagc gggagaaaat caaccgcagt ctggatgtgc tggaaggata
      481 tctggtcgat ggcacactca aaaccgatac ggtcaatctg gcgactatcg ccattgcctg
      541 tgctgtcgga tatctcaatt tccgccgcgt tgcgccaggt tggtgtgtcg accgcccaca
      601 cttagtcaaa ctggtcgaaa acctgtttag ccgcgaaagt tttgctcgca ccgaaccgcc
      661 aaaggcttga tgcgcgatat gtcctcctga cccatctcac gttacaatcc gtggttatgt
      721 taaacgccct tctccgtgtg agagggcctt gatcagccag gtttcctatg acaaccgaaa
      781 cgcgttccct ctatagtcaa cttccggcta ttgatcgctt attgcgcgat agctccttcc
      841 tttctttgcg tgatacttat ggtcacaccc gcgtggtgga attgttgcgt cagatgctcg
      901 acgaagcgcg agaagtgatt cgtgacagcc agacgcttcc tgcgtggtgt gaaaactggg
      961 cgcaagaagt cgatgcccgg ttgacgaaag aggcgcagag cgcgctgcgt ccggtgatca
     1021 acctgacggg aaccgtgctg cataccaacc ttggacgagc tttacaggcg gaagccgcgg
     1081 tggaagccgt tgcgcaggct atgcgttcgc cagtgaccct cgagtacgat ctggacgacg
     1141 ccggacgcgg acatcgcgat cgggcgctgg cgcaactgct gtgccgtatt acgggggcgg
     1201 aagatgcctg tatcgtcaat aacaatgcgg cggcggtgtt attgatgttg gcggccactg
     1261 ccagcgggaa agaggtggta gtatctcgcg gcgaattggt ggagattggc ggcgcgtttc
     1321 gtattcccga tgttatgcgt caggcaggct gcaccctaca cgaagtaggg accaccaacc
     1381 gcacgcacgc gaatgattat cgtcaggcgg tgaatgaaaa taccgcactg ttgatgaaag
     1441 tacataccag taactacagc attcaggggt tcaccaaagc gatagatgaa gcggaactgg
     1501 tggcgctcgg caaagagctg gatgttcccg tagtgactga tttaggcagt ggctcgctgg
     1561 tcgatcttag ccagtacggt ttgccgaaag agccaatgcc gcaggagttg attgcggcgg
     1621 gcgtcagtct ggtgagcttc tccggcgaca agttgttagg cgggccgcag gcagggatta
     1681 tcgttggtaa aaaagagata atcgcccgcc tgcaaagcca cccgctgaag cgtgcattac
     1741 gcgcggataa aatgaccctc gcggcgctgg aagccacgtt gcgtctttat ttacaccctg
     1801 aagctctgag tgaaaaatta ccgaccctgc gcctgcttac ccgcagcgca gaggtcattc
     1861 aaatccaggc acaacgttta caggctcccc ttgccgcaca ttacggcgcg gagtttgcgg
     1921 tacaggttat gccatgtctt tcgcagattg gcagtggttc gctgccggtt gatcgcctgc
     1981 cgagcgcggc attaacgttt acaccccatg atggacgcgg tagccacctt gagtcattag
     2041 ccgcccgctg gcgtgaattg ccagtgccga tgattggtcg tatttatgac ggacgattgt
     2101 ggctggattt acgctgcctt gaagatgagc aacggttttt ggagatgttg ttgaaatgat
     2161 tattgcgact gccggacacg ttgaccacgg caaaacaacc ttattgcagg tgattactgg
     2221 cgtaaatgct gaccgtctgc cggaagaaaa aaagcgcggc atgaccatcg atctcggcta
     2281 tgcctactgg ccgcagccgg atggtcgcgt gcctagtttt atcgacgtac ccggtcatga
     2341 aaagtttctt tccaacatgc tggcgggcgt tggtggtatc gatcacgcgc tgttggtggt
     2401 ggcgtgcgat gacggcgtga tggcgcagac ccgtgagcat ctggcgattt tgcagctgac
     2461 cggtaacccg atgctgacag tggcgctgac caaagccgat cgcgtggacg aagcgcgtgt
     2521 tgatgaggtt gaacgccagg taaaggaggt gctgcgggaa tacggttttg ctgaggcaaa
     2581 actgtttatc accgcagcaa ccgaaggtcg gggaattgat gccctgcgcg agcatctgct
     2641 tcagttacca ggacgtgaac acgccagcca acatagtgtc cgcctcgcga tcgaccgcgc
     2701 atttaccgta aaaggtgccg ggctggtcgt caccggtacg gcgttaagcg gggaagtgaa
     2761 ggtaggcgat tcactctggc tgactggtgt aaataaaccg atgcgtgtac gtgcgctgca
     2821 tgcacaaaac cagccaacag aaaccgccca tgccgggcag cgtatcgcgc ttaacatcgc
     2881 gggtgatgcg gaaaaagagc agattaaccg tggcgactgg ctgcttgccg atgcgccgcc
     2941 agagccgttc acacgggtga ttgtcgagct tcaaacccat acgccgctga cccagtggca
     3001 gccgctgcat attcaccacg ccgccagcca cgttacggga cgcgtttcgc tgctggaaga
     3061 taaccttgcc gagctggtgt tcgacacgcc gctgtggctg gcggataacg atcgcttagt
     3121 gctgcgcgat atctcggccc gcaacacgct ggcgggggcg cgtgtggtga tgcttaaccc
     3181 gccgcgtcgc ggtaaacgta agccggaata tctgcaatgg ctggcgtctc ttgcacgggc
     3241 gcagagcgat gccgatgcgt tatctgttca tctggaacgc ggcgcggtta accttgcgga
     3301 tttcgcctgg gcgcgccagc tcaacggcga agggatgcgc gaattgctgc aacagcctgg
     3361 ctatattcag gctggttata gcttgttgaa tgcgccggtt gccgcccgct ggcagcggaa
     3421 aattctcgac acattagcga cttatcatga gcaacatcgc gatgaacctg gtcctgggcg
     3481 cgaacgtctg cgacgtatgg cgttgccaat ggaagatgaa gcgctggtac tgttgctgat
     3541 tgaaaagatg cgcgaaagcg gcgacatcct cagtcatcac ggttggctgc atctgccgga
     3601 tcacaaagcg ggcttcagcg aagagcagca ggccatctgg caaaaagcag agccgctgtt
     3661 tggtgacgag ccgtggtggg tgcgtgatct ggcaaaagag acgggtaccg acgagcaggc
     3721 aatgcgcctg acgctacgcc aggcggcgca gcaaggaata attaccgcga tcgtcaaaga
     3781 tcgttattac cgtaacgatc ggattgtcga atttgccaat atgatccgcg atctcgatca
     3841 ggagtgtggt tcaacctgcg cggcggattt ccgcgatcgc ttaggcgtag gccgaaagct
     3901 ggcaattcag attctggaat attttgaccg cattggcttt acgcgtcgtc gtggaaatga
     3961 tcatttattg cgcgacgcat tattatttcc ggaaaaataa ggaaatgatt aattaatttt
     4021 taaaataaat taatacaaat ttctggtaat gactccaact tattgatagt gttttatgtt
     4081 cagataat
//
LOCUS       DAZRVY010000233         4087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZRVY010000233 DAZRVY010000000
VERSION     DAZRVY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.236"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="ACHXOX_004200"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="ACHXOX_004200"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR7007512.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="ACHXOX_004201"
     CDS             930..1382
                     /locus_tag="ACHXOX_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR7007513.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="ACHXOX_004202"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="ACHXOX_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHXOX_004203"
     CDS             complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHXOX_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR7007514.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHXOX_004204"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHXOX_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR7007515.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          899 a          906 c         1077 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactggt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAZRVY010000234         4078 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZRVY010000234 DAZRVY010000000
VERSION     DAZRVY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.237"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..786
                     /locus_tag="ACHXOX_004205"
     CDS             <1..786
                     /locus_tag="ACHXOX_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7007516.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            799..1758
                     /locus_tag="ACHXOX_004206"
     CDS             799..1758
                     /locus_tag="ACHXOX_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR7007517.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="ACHXOX_004207"
     CDS             complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="ACHXOX_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR7007518.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="ACHXOX_004208"
     CDS             complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="ACHXOX_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR7007519.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1039 t
ORIGIN      
        1 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac gttatcgatg aaaacaaaac atatatatgc
      781 cagtaaaagg agtttactat gaagtggctt ttattaatca ccttgagtct atattctttt
      841 attgtgcaaa gtgctccctg tgcattgacc aatgtcgggg aacaacgtgg aacatatata
      901 ctcaagccac tgagtatgaa aggtaatctc accgccgaaa aaattgaaaa caccggaaat
      961 atcattgagt gtgcagactt acaagagacg accaacgcca ttaaagttca ttttttaaca
     1021 cagaacgctt taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa
     1081 tctgacggta aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt
     1261 acagggaata atactaattg taatagcgtc cgctttaatt ataatttaaa tactatttta
     1321 caggtaaatt taatgacctg tggttttgaa gatcaatcca tcgacactgg acgatttgta
     1381 ctgtctcgta tcaataatga aaattatcag tttcatcata taagatttga ctgcatccag
     1441 ggcgagcaag gtgaattagt ttttgaaccc agtgatgttg aatattactt tgaaccagtg
     1501 acgatacagg agtcaggaac cagcatatta aaaaatgagt tagaaaacag tgaggacggt
     1561 gcgggtcaag taggctttca actctccaat gatggtacga atgaaatcca gtatggtaaa
     1621 agcaattact actcttttca gcatccccat gaaggaagta atcagattcc tctatttatt
     1681 cgcccgcgta catatggaaa taatgtctca agcgggcaaa taatgagccg agtcaaaatc
     1741 gtggttatgt acaactaatc tatctccccc accttaactt tgggggaggt gccgattaac
     1801 gcgccgctgg actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt
     1861 gctgaatcat ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac
     1921 caatttctac gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga
     1981 agattgcatt gtgcatcgca ccaaaagctt tcatcaacgc ggcatcatca aactcgccga
     2041 ggatttctac gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg
     2101 aacgtcgccc aggaatcaaa agtcgccgtt cttccagaca agccgggaac gatttttctg
     2161 gtggtggatt tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc
     2221 cttcctgctg cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac
     2281 ttaattgctc cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc
     2341 cttctaccac tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca
     2401 cgccaacgtc aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct
     2461 ggcttaaggt gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt
     2521 cgagaccacg tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa
     2581 tctgtccggt aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac
     2641 cttctttata gacatgccag aagtaataca agtggttgta attgatatga gacatgctca
     2701 tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg
     2761 tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat
     2821 agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa
     2881 ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccgc
     2941 cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa
     3001 ccagcagaac agactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa
     3061 tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc cagcattagc
     3121 aaatgcaaac agcggtaaaa tcagatacgc cacccacggg tgcaatacat gctccagtcg
     3181 cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc
     3241 cgccagggtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca cgccaacaag
     3301 aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc
     3361 tacagccgcg acgccaagag aggccatcga taagtcatta gtgtagaaca atgcgatgat
     3421 aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagtgctaa
     3481 cggaacacga cttcccaaca gcgccagcac accaagtgca aaggcaatgt cagtagccgc
     3541 cgggatcgcc cacccttcgc gggtaatcgg atcggcatag ttaaaggcca gatagagtaa
     3601 tgccggaaca atcatcccac caatagcggc gataaccgga aatgcggcct ggcgtaagct
     3661 ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac
     3721 cgccatcagc gcgtcattta tccataacag catgtttttg ttgatttcga gtgaaccaac
     3781 ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctgtt
     3841 ggccatcatc atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact
     3901 gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt
     3961 ttactatttt accccgcaca aatcatcgtt aaaatcagat tatagtgtag gaataactcg
     4021 ttttttacga gcaattaaag gatttgcgcc atcagcggtg agatgagttt ttgacggc
//
LOCUS       DAZRVY010000235         4059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZRVY010000235 DAZRVY010000000
VERSION     DAZRVY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.238"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..782
                     /locus_tag="ACHXOX_004209"
     CDS             <1..782
                     /locus_tag="ACHXOX_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR7007520.1"
                     /translation="SVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSLH
                     REAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQL
                     QTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVIN
                     INSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSSE
                     DHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            779..1684
                     /locus_tag="ACHXOX_004210"
     CDS             779..1684
                     /locus_tag="ACHXOX_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR7007521.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1681..2751
                     /locus_tag="ACHXOX_004211"
     CDS             1681..2751
                     /locus_tag="ACHXOX_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR7007522.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2988..3341
                     /locus_tag="ACHXOX_004212"
     CDS             2988..3341
                     /locus_tag="ACHXOX_004212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007523.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            3467..4006
                     /locus_tag="ACHXOX_004213"
     CDS             3467..4006
                     /locus_tag="ACHXOX_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR7007524.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1100 g          982 t
ORIGIN      
        1 ttagtgtcag tgccccgggc catcattcat ggaaacgaga ctttgatccg gacagccccg
       61 agataaaatt cagtgatcgc agggaagccc ttaaactgat gacgcaaatc gaatcgaccg
      121 tgaccagcct gcaccgtgag gctgaagcac agttccggcc tgaactggaa aaaatcgtca
      181 ggggtatcga aacaggtttt cgtggtacgg ccctgtatgc cacagaaaat attgccggac
      241 gtatcaatgc ccgcctggag gacgagggct tcactgtaaa aatcactttc ccggcagtca
      301 gccagttaca gaccaggatc gcggtaaaaa caaatctgag tgcgcttatg gaggaaagaa
      361 ctgagacagt cacccgtcgc cgtcggcaga gtggtttctg gggaaagatt tgtggagcgt
      421 ttggcaccag tgactggggc tgggaaaact ataaggagaa tgtgagccgc agtgtgatca
      481 atatcaactc ggtcaggaag gaagtgatgt cactgacccg ggcatatttc ggggagttgc
      541 aggcatccat tgagcagaat attaaccagc ccgtccgcca ggagatcgat gattttttct
      601 gtacattcag ggaaaaagtc gaacagctac gtaacacact cattcagagc tctgaagatc
      661 ataaacgcga tcagcaggca caggagcagc ttacggagcg ccttcaggca ttaaatgaaa
      721 gggttcccga gctgattact gacagtaagg cgttaaggga agaactggag acactgctgt
      781 gacctcacca tttattcaac aaattgctga taaccgggta tgtcaggtac tcagttgcct
      841 tcctgaaaaa tttgtggttg attttgcaaa cggtattgat gttgcacagg agcataaccg
      901 cacggccggg gggcgcacgt ttttccggcg tttaaaagaa ggtctgactg gcaaaggcgc
      961 tgtacgccag aatgccatca atgcttcgct tgcacagggc gttgaggcgt ccctgcgctg
     1021 gctgacggag ctgacaacgt cgcttgccac cacaaattac gcgattaccc gggtaaacga
     1081 cagggtcagt tcactggtca gtgatactgc caggctggcg cattattcgg cagacacgcg
     1141 ggagcagtta ctcaccctgg cagaacaggt tcatcagaaa ctgaatcatc ttgaagaaaa
     1201 actccaccgt gttgaccagg ttcagcgggc acagttacat cttgagcaga tattctcatg
     1261 gtggagcgcc gggcgatacg cgtcattttc ccctgccgga cgttgttatg tggcgcttga
     1321 agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag ggtgaaacag gccaggttaa
     1381 ccagttactg gatatcctca gacataaagc attaacgcaa atggcacagg agaatggcgg
     1441 tagcgcaaca gtacgtctga acacactgga ctggcttggt ggtcagagcc gggaacaggc
     1501 tgataacgaa tggcatgaag cggttaactg gctgggggac tggtgcagtg aagagcggca
     1561 tcctgtgatc tggtcaacca cacaggctgc agaacactta ccggttcgta tgccccgtct
     1621 ctgctctgca gaacgcctct ctgaaagtat ggttgatgaa atatttcaga agggggaggc
     1681 atgagtacag aaatgaaaac ggggctggtg ctgtccggcg gcggggcggt gggcgcttat
     1741 caggcgggag tggttaaggc actggcagag tgcggcgcac agatcagtat ggtctcagga
     1801 gccagtatcg gggctctcaa tggtgccatt atcacggcct ctcccgatct gtcagaagcc
     1861 gccttacgcc tggaggcgct ctgggatcat ctggggaata atcaggttct gtcggtaaac
     1921 agatcggttt acttttcatt gctgaaaaaa ctggttcagg ccatgaacct ctgccagatc
     1981 cccggacgtg cgggcgcact gcttacgacg ctttttcgcc atatatcgac aatcaacggg
     2041 ttcgacaatc cgatgattca gccgctgttg tcagatgagc ccctgacagc gctgatggat
     2101 cattatcttg atacagatgc tctggccgac gggctaccgc tgtacgtgtc gctgtacccc
     2161 acagaaggag gcatgcagga tattattgac tgcattcgtg ctgaactggg tgccggaacc
     2221 acgaaaaacg ctgtttttca gcatatccag agtctgcccc gcgggcagca gaaagaagcc
     2281 ttactggcgt cagccgcgct gcctctgctg ttccgtcccc gcgaggttca gggaacaatg
     2341 tacggtgatg gtgggatggg cggatggcga aacaggcagg ggaatactcc cgtgacgcca
     2401 ctggtggatg ccggatgcaa tatggtgatt gtgacgcatc tgagtgacgg ttctttatgg
     2461 gatcgtcggg cttatccgga caccacaatc cttgaaatcc gtccccggaa aaggttgaaa
     2521 caaatcgggg acgaaggcaa aagtggcggt ctgctcagtt ttacatcggc acataccgac
     2581 gcctggcgtc agcagggcta cgaggacacg atgctgacga tggagcatat ccggaaaccg
     2641 ctggcagcac gtcaggcact gacccggtca gagacggtat tgcagaaaag cctggagata
     2701 actgaaggtg cagattcggc actgagaaac gcgatggccc ggattaaata aagacgctcc
     2761 ggagaaacgc caccgccact gtggtggtgt ttttcctgtc tggtattatt gaaaatattt
     2821 tcagatatat attacctgga tgatggttaa gattaattta tttaattttg gggagaaaac
     2881 tcatgaaact gttaaacatt cttttagctg ttgttttaac tgtaagtgca accgtagcac
     2941 aaacagaatc ggcattgtag acgcgtaaca gtattgctgg cactgatatg ttcagtatcg
     3001 gtaatgtttc tattaactgc acgattaatc cagaagacaa caatttgcta ccacactatg
     3061 tgtggattta cggtcatacc tccacccagc taaatcagta cgattctgat attattttta
     3121 tcatcaacga taaaaagtat ccggccccat ctgtcgacgg aacacgaatg aataagaatg
     3181 catgggtata ctttatcgat gctatcagtg aagccacgca attcgacgtt ttagtaaacg
     3241 gtaagaaagt cgacagctat accgcaaata tcaaaaatgt gaaaaagact ttgggtaaca
     3301 aattctacga cagctgctgg aacacctggt tccaggaata attgataacg tggtgtaaaa
     3361 cacctcagtt taatttaata caatgagtga aaatgaatat tataaaaata ttttttgttt
     3421 cagatgtcat atattttatt aactgaatga ttaacaggag aatcagatga actggcatta
     3481 tgaaaaaaat ggtgtacgtt atgataacgt aacagaggaa gatattatcg aacgtatccg
     3541 gcgtggtgaa ctgaatgcat caacactggt gtggcaacag ggcatgctgg aatggcagcc
     3601 agtttcagcc acatcactgg cggaaatact gaaacaaagt gaagttcccc cagcccttcc
     3661 gggtcaccgc attcccggag gcgttgtctg gacgctggca tttgcccctt tgattgggta
     3721 catacttgag atgtggactg cagggctgaa cggtatgtca tttgaacggg cttatgatgc
     3781 agtcagcgaa ggccagtact ggtttatctc tctgattctg aatattgctc tgggatatct
     3841 ggatgaacga aaactccgta aatctggtgt tgatactgca acatttggct ggctggcatg
     3901 gttgatcccg gtttatctgt ggcgtcgggc aaaaatcctc ggacaaaaac ctgcgtattt
     3961 ctgggtctgg ttggttatgc tgatactggt gttatttacg gtctgatatt ctttatcgca
     4021 gagtttacta aataatcagg ttattagtgt tcttaacag
//
LOCUS       DAZRVY010000236         4045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZRVY010000236 DAZRVY010000000
VERSION     DAZRVY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.239"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHXOX_004214"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHXOX_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR7007525.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHXOX_004215"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHXOX_004215"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR7007526.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHXOX_004216"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHXOX_004216"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR7007527.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHXOX_004217"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHXOX_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR7007528.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1141 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAZRVY010000237         3993 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZRVY010000237 DAZRVY010000000
VERSION     DAZRVY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.240"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHXOX_004218"
     CDS             <1..507
                     /locus_tag="ACHXOX_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7007529.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..982
                     /gene="yqeJ"
                     /locus_tag="ACHXOX_004219"
     CDS             500..982
                     /gene="yqeJ"
                     /locus_tag="ACHXOX_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR7007530.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="ACHXOX_004220"
     CDS             complement(1016..1096)
                     /locus_tag="ACHXOX_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007531.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHXOX_004221"
                     /pseudo
     CDS             complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHXOX_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1986..2476
                     /locus_tag="ACHXOX_004222"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="ACHXOX_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2701..3192
                     /locus_tag="ACHXOX_004223"
     CDS             2701..3192
                     /locus_tag="ACHXOX_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7007532.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3527..3982
                     /gene="ygeH"
                     /locus_tag="ACHXOX_004224"
                     /pseudo
     CDS             3527..3982
                     /gene="ygeH"
                     /locus_tag="ACHXOX_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
BASE COUNT         1494 a          639 c          645 g         1215 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat
     2521 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
     2581 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
     2641 aatattaatg agtcaatcaa taataattta gcatcacgag acacatcaca gaggaatatt
     2701 atgagcacag aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa
     2761 cacgcattat cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt
     2821 gatagaatat atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa
     2881 atttattata aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt
     2941 gcatcagtat gtcagccaaa aaagaaatat caacaagcat atgaccttta caaactaagt
     3001 tacaattact ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt
     3061 ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag
     3121 gatgatagtg ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa
     3181 gataatggct aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacta
     3241 ctataaccag cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac
     3301 tataaatcat tatttttcaa tgcgttagag aacaatctac tttataaaaa ttgaataagg
     3361 ctatttcaga ttacatcact gaattcctaa gataaattag tgaacacaac catagatatg
     3421 tcgaatgtat tttcctgtag cattaatgac acataaatat gaatagccat aatttctatt
     3481 gcttaagcag gatgcaagaa accaattttt tcatagaggt taactaatgg acttagaaaa
     3541 taaattctca tatcattttc ttgagggatt aacgctcacg gaagatggaa ttcttactca
     3601 aggaaatgag caagtttata ttccacagaa agagttaggt gtattaatag tattacttga
     3661 atccgctggt catgttgtac tgaaagatat gatcatcgaa tcagtatgga aaaatattat
     3721 tgttagtgac gagtccctga caagatgtat ctattctttg cgctgcattt ttgaaaaaat
     3781 tggctatgat cgttgcatag aaacaatcta ccggaaaggt tatcgtttca gcgggcaggt
     3841 tttcaaaact aaaattaatg aagataatac ttcagactat tccatagcta tattcccttt
     3901 cactacttca ttgaatacac tggatccatt aatacttaat caggaattag tgcaaatcat
     3961 ttcaaataaa aaaatcgatg gttagactgg ccc
//
LOCUS       DAZRVY010000238         3959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZRVY010000238 DAZRVY010000000
VERSION     DAZRVY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.241"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHXOX_004225"
     CDS             complement(<1..242)
                     /locus_tag="ACHXOX_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7007533.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHXOX_004226"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHXOX_004226"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR7007534.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHXOX_004227"
     CDS             2255..3904
                     /locus_tag="ACHXOX_004227"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR7007535.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZRVY010000239         3947 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZRVY010000239 DAZRVY010000000
VERSION     DAZRVY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.242"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            32..970
                     /locus_tag="ACHXOX_004228"
     CDS             32..970
                     /locus_tag="ACHXOX_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR7007536.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1026..2315
                     /locus_tag="ACHXOX_004229"
                     /pseudo
     CDS             1026..2315
                     /locus_tag="ACHXOX_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2312..2605
                     /locus_tag="ACHXOX_004230"
     CDS             2312..2605
                     /locus_tag="ACHXOX_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR7007537.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2770..3947
                     /gene="fadK"
                     /locus_tag="ACHXOX_004231"
                     /pseudo
     CDS             2770..3947
                     /gene="fadK"
                     /locus_tag="ACHXOX_004231"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          930 a         1004 c         1088 g          925 t
ORIGIN      
        1 gccctgaact gaagcctacg gagaagaaac gatgagtcaa ttaaacagcg tctgggtctt
       61 tagcgataat cctgaacgtt atgctgaact gtttggcggc gcgcagcaat ggggccaaca
      121 ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca gttatgcctt atggtccaaa
      181 atgtatttat gttcttgagc aaaatgatgc gctgcaacgt actgaaaatt acgccgaaag
      241 cattgctgcc ctgctgaaag ataacccccc cgctatgttg ctgctggccg cgacgaaacg
      301 tggtaaagcg ctggcagcac ggttaagtgt gcaactgaat gcggcgctgg tgaacgatgc
      361 cacggcagtg gatattgtcg atggtcacat ttgcgccgaa caccggatgt atggcgggtt
      421 agcgttcgct caggaaaaga tcaacagccc gctggcgatc attacccttg cacccggtgt
      481 tcaggaaccg tgcaccagtg atacctctca tcagtgcccg acagaaacgg taccttatgt
      541 tgccccgcgt catgaaattc tctgtcgcga acgccgtgcg aaagcggcaa gcagcgtgga
      601 cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt ctggcagcgc aggatgacct
      661 gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa gtcggctgtt cacgtccaat
      721 tgccgaaggc gagaactgga tggagcgtga acgttatatc ggtgtctccg gcgtgttgct
      781 gaaatccgat ctctacctga cgctggggat ctccgggcag atccagcata tggttggcgg
      841 caacggcgca aaagtgattg tcgccatcaa taaagataaa aatgcgccaa tcttcaatta
      901 tgccgactac ggtctggtgg gcgatatcta caaagtcgtc cctgccctga ttagccagtt
      961 gagccgctaa ttcccctttc aaccacatct cccgctgtaa acgcagcggg aagggagcat
     1021 aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg tgcgggcgtt gctggtagcg
     1081 ttgccgcact ggtcatggcg cgagccgggc tggatgtcct ggtgatagaa cgcggcgaca
     1141 gtgccggatg taaaaacatg accggcgggc gtctttatgc ccacacactt gaagcaatca
     1201 ttccaggctt tgcagtatca gcgccggtag aacgcaaggt cacacgcgag aaaatctcct
     1261 tcttaaccga agaaagcgcc gttaccctcg attttcaccg cgagcagcca gatgttccgc
     1321 aacacgcatc ttataccgta ttgcgtaatc gtctggaccc gtggttgatg gaacaagccg
     1381 agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga tgcgctggtt cgtgaaggaa
     1441 acaaggtaac tggcgtgcag gctggggatg atattctcga agcgaatgtg gtgattctgg
     1501 ctgatggcgt taactcaatg cttggccgct cgctgggaat ggttcccgct tccgatccgc
     1561 atcattacgc tgttggtgtt aaagaggtta ttggcctcac accagaacag atcaacgatc
     1621 gctttaatat tacgggcgag gaaggtgccg cctagctgtt tgccggttcc ccttctgacg
     1681 gcctgatggg tggcggattc ctctatacca acaacgattc cgtatcgttg gggctggttt
     1741 gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc gcaaatgctg gaagatttta
     1801 aacaacaccc cgccattcgc ccactaatta gcggcggcaa actgcttgaa tattccgcgc
     1861 atatggtgcc ggaaggcggt ctggcgatgg tgccgcaact ggttaacgag ggcgtgatga
     1921 tcgttggtga cgccgcaggc ttctgtctga atttgggttt tacggtccgc ggcatggatt
     1981 tagccattgc atcggctcag gctgccgcca caacggtgat cgccgccaaa gaacgcgagg
     2041 atttctccgc cagcagtctg gcgcaataca agcgtgagct ggaacaaagc tgcgtcatgc
     2101 gtgatatgca gcattttcgc aagatcccgg cgctgatgga aaacccgcgc ctgtttagcc
     2161 aatacccacg aatggtagcc gacatcatga acgagatgtt caccatcgac ggcaaaccaa
     2221 accagccggt acgaaaaatg atcatgggac atgcgaagaa aattgggctg atcaacttgc
     2281 tgaaagatgg cattaaggga gcaaccgcgc tatgagccag aacgctacgg ttaacgtcga
     2341 catcaaatta ggcgtcaata aattccatgt tgatgagggc cacccgcata tcattctggc
     2401 ggcaaatccc gacatcaatg aattccgtaa attaatgaaa gcctgccctg ccggacttta
     2461 taagcaggat gacgccggca acattcattt tgattctgcc ggttgtctgg agtgcggcac
     2521 ctgtcgggtg ctgtgcggta acactattct cgaacagtgg caatatcccg caggcacctt
     2581 cggtattgat tttcgctacg gctaatcatg catcccacag gcccgcatct cgggcctgat
     2641 gttctgtttc gagagtccaa catgaaagtg acattaacgt tcaacgaaca acgtcgtgcg
     2701 gcgtatcgtc agcaagggct atggggcgat gcttcgctgg ccgattactg gcagcagacc
     2761 gctcgtgcga tgccagacaa aattgccgtg gtcgataatc atggtgcatc gtacacctat
     2821 agcgcgctcg atcacgccgc gagctgtctg gcaaactgga tgttagcgaa gggtattgaa
     2881 tcaggcgatc gcatcgcatt tcaactgcca ggctggtgtg aatttaccgt tatctatctt
     2941 gcctgcctga aaaccggtgc ggtttccgtg ccgctgttgc cttcctggcg ggaagcagaa
     3001 ctggtgtggg tgctcaataa gtgtcaggca aaaatattct ttgcaccgac gttgtttaaa
     3061 caaacgcgtc cggtagattt aatcctgccg ctgcaaaatc agcttccaca actacaacaa
     3121 attgtcggcg tggacaaact ggctcccgcc acctcttccc tctcattaag tcagattatc
     3181 gccgacaata tcccactgac cacggcgata acgacccacg gcgatgaatt agctgcggtg
     3241 ctgtttacct ccggaaccga gggtctgcca aagggcgtaa tgctaacgca taacaatatt
     3301 ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga cctggcagga tgtctttatg
     3361 atgcctgcgc cacttggtca cgcaacggga tttctgcatg gcgtaacagc accattttta
     3421 attggtgctc gcagcgtgtt gttagatatt ttcactcctg atgcatgtct cgcgctgctt
     3481 gagcagcagc gttgcacctg tatgctcggc gcaacgccgt ttgtctatga tcttttgaat
     3541 ttactagaga aacaacccgc agacctttca gcgctgcgct tctttctttg cggcggtacc
     3601 acaatcccca aaaaagtggc gcgcgaatgc cagcagcgct ggcattaaat tattaagtgt
     3661 ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat ctcgatgatc ctttgtcgcg
     3721 ctttatgcac accgatggtt acgctgccgc aggtgtagag attaaagtgg tcgatgacgc
     3781 acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc tcgcgtggcc ccaatgtgtt
     3841 tatggggtat tttgatgaac ctgaattaac cgcccgtgcg ctggatgaag aaggctggta
     3901 ttacagcggc gatctctgcc gtatggatga ggctggctat ataaaaa
//
LOCUS       DAZRVY010000240         3938 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZRVY010000240 DAZRVY010000000
VERSION     DAZRVY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.243"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            36..587
                     /locus_tag="ACHXOX_004232"
     CDS             36..587
                     /locus_tag="ACHXOX_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR7007538.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            647..3151
                     /locus_tag="ACHXOX_004233"
     CDS             647..3151
                     /locus_tag="ACHXOX_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7007539.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAVVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3167..3916
                     /locus_tag="ACHXOX_004234"
     CDS             3167..3916
                     /locus_tag="ACHXOX_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7007540.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
BASE COUNT         1225 a          821 c          802 g         1090 t
ORIGIN      
        1 ttcattttct acttatattt atataaagga atattatgtc tgcttttaaa aagtcacttc
       61 ttgttgctgg cgtagcaatg attctttcca ataacgtatt tgctgacgaa ggtcacggca
      121 ttgttaaatt taaaggtgaa gttatctctg ctccatgttc aattaaacca ggtgacgaag
      181 acttaactgt aaatctgggt gaagtagcag ataccgttct gaaaagcgac cagaaatctt
      241 tggctgagcc tttcactatt cacctgcaag actgcatgtt gagccagggt ggaaccactt
      301 attcaaaagc gaaggttact ttcaccacag cgaatactat gacgggtcag actgacctgc
      361 tgaaaaacac caaagaaact gaaatcggtg gtgccacagg cgttggcgta cgtattctgg
      421 atagccagag cggcgaagtg actttaggta ctccggtagt aattacattc aacaatacta
      481 acagctatca ggaactgaac ttcaaagctc gtatggaatc cccgggcaaa gatgctaccc
      541 cgggcaacgt ttacgctcag gctgactata agattgctta cgagtaatca cattaggccg
      601 ggttcgcccg gccacatcat taacggatta atgataagtg gaacagatgt ataaaaaatt
      661 aaaattaacc acaataagcg aattgattaa aaatatttat tgttcattat ccgttctcat
      721 cattggttgt gcgtcagctt atgccgttga attcaacaaa gatttaatcg aagccgaaga
      781 tcgtgaaaac gttaaccttt cccaatttga aactgatggc caattacccg taggtaaata
      841 ctcattaaac gctcttatta ataataaaag gacgccaatc catctggacc tgcaatgggt
      901 attaattgat aaccaaactg cagtttgtct gactccagag caattaacat tattaggctt
      961 tactgatgag attattgaag aggctcagca aaacctgatc gatggttgtt accctatcga
     1021 aaaagaaaaa caaattacaa cttatctcga taaagggaaa atgcaattat ccatatctgc
     1081 acctcaggca tggttaaaat acaaagatgc aaactggacg cctcctgaac tttgggatca
     1141 tggtattgct ggggcatttc ttgactacaa tttatatgcc tctcattatg caccacatca
     1201 gggcgataat tcacaaaata taagttccta tgggcaggct ggggttaatc ttggggcctg
     1261 gcgcctgcgt actgattacc agtacgatca gtcatttaac aatggcaaaa gccaggcgaa
     1321 caacctggat tttccgcgta tttatttgtt tcgcccaatc ccagcaatta atgcaaaact
     1381 aactataggt caatacgata ctgaatcctc tattttcgac tctttccatt tttctggcgt
     1441 ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta cgtggttacg caccgcaaat
     1501 cacgggtgtt gcacaaacga atgcaaaggt cactgtctca cagaacaacc gtattattta
     1561 tcaagaaaat gttcctcctg gcccatttgc tattactaat ttattcaata cattacaggg
     1621 gcaactggac gtcaaggttg aagaagagga cgggcaagtc acgcaatggc aagttgcatc
     1681 taatagtatt ccttatctga cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa
     1741 accgaccagc gttggtggtg attccttaca acaacccttc ttctggactg gtgaattctc
     1801 atggggttgg ctgaacaatg tatccctgta tggtggttca gttttaacaa accgtgatta
     1861 tcaatctctg gctgccgtcg ttggctttaa ccttaactca ttgggttcat tatcttttga
     1921 tgtcacacga tctgatgctc agttgcataa tcaggataaa gaaacgggtt atagctaccg
     1981 cgctaactat tcaaaacgtt ttgaatctac cggtagccag ctcactttcg ctggttaccg
     2041 tttctctgat aaaaactttg tgacaatgaa tgaatatatc aatgacacta accattacat
     2101 gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc ttgaatcatt
     2161 aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca gcagtaatgt
     2221 gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa acgtctccgc
     2281 ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac tgtacctaaa
     2341 tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac gaaatcagga
     2401 taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata attcctggag
     2461 cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac tacgcgccag
     2521 ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac agcgagacag
     2581 ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc acggagccgc
     2641 tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag atggtgcaga
     2701 agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg gagttattcc
     2761 atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc ttccagaaaa
     2821 tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta ttggctacgc
     2881 caaacttgat acccgcaagg gctatcaaat catgggggtt attcgcctgg cagatggtag
     2941 tcacccacca ctggggatta gcgtaaaaga taaaaccagc cataaagaat taggactggt
     3001 tgctgatggc ggctttgtat acctcaacgg cattcaggat gacagcaaac ttactttacg
     3061 ctggggtgat aaatcctgtt ttattcaacc acccaatagc agcaacttaa ccaccggaac
     3121 ggttatttta ccgtgtatta gccaaaatta attcaggatg aaactaatgc gctacttaaa
     3181 cacaaagaat ctcattgcag caggagtatt actcgcctgc atgagcagca ttgcctgggg
     3241 tgccatcatc ccggaccgta cccgtatcat aatgaatgag tcagataaag gggaagcatt
     3301 aaaacttact aatcaaagca aaaatctacc ctatctggcg caaacatgga tagaagatac
     3361 gaaaggcaat aaatcacgtg actttattgt cacggttccc cctatggtac gtttaaatcc
     3421 cagtgagcaa attcaaataa gaatgataag tcaggaaaaa attgctcaac tgcctaatga
     3481 cagagaaacg ctcttctatt ttaacgtgcg agaaataccg ccaaaaacag ataaaaaaaa
     3541 tgtgatgcaa gtcactatgc agcacgcatt gaaactattc tggcgaccaa aagctattga
     3601 attagaagat gatggggtaa tgtcttacga aaaagttgaa atcatccgta gaaatgatgg
     3661 ttcgatccgc tttaacaata agatgcctta tcacgttacg ctgggttata tcggaacaaa
     3721 cggaatcaca atgttgccac aaacgcaaag tttaatggta accccttata gccatgcaga
     3781 tacgcagttt aaaaatgttc cgtcagcttt tcaggttggt tatatcaatg attttggtgg
     3841 attaagtttt tatgaaataa actgcccgac ggttaataac tcttgtaatg tttccgtagc
     3901 caaacgagat aaataatatg cgctacttat taattgta
//
LOCUS       DAZRVY010000241         3936 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZRVY010000241 DAZRVY010000000
VERSION     DAZRVY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.244"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..585
                     /gene="fimZ"
                     /locus_tag="ACHXOX_004235"
     CDS             <1..585
                     /gene="fimZ"
                     /locus_tag="ACHXOX_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR7007541.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHXOX_004236"
     CDS             complement(588..1103)
                     /gene="sfmF"
                     /locus_tag="ACHXOX_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR7007542.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHXOX_004237"
     CDS             complement(1114..2121)
                     /gene="fimH"
                     /locus_tag="ACHXOX_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR7007543.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHXOX_004238"
                     /pseudo
     CDS             complement(2134..3908)
                     /gene="fimD"
                     /locus_tag="ACHXOX_004238"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1094 a          971 c          861 g         1010 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaaactagg tttgcattca
      541 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
      601 atcataaata tcgcatccgc tttctccaca cctggctgaa caccgctggc taacgctcga
      661 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
      721 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
      781 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
      841 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
      901 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
      961 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
     1021 ttaatcgttc ccaaagggtc agctgcccat ccactggaac tccacagtaa cccgcagaaa
     1081 cagctaaaga gtactcttct cattctccgt acctcattga taatcgacgc gtaaataccc
     1141 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
     1201 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
     1261 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
     1321 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
     1381 cgcagcaaca ttcgtacatt tgatcgcaat gctcttggtt tgcggcatga cgccagctgg
     1441 tcgattcccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
     1501 aacaatctgc ccggcattta attcgcagtt ttgcggcact tcaacccggc caccaaaact
     1561 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
     1621 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
     1681 tatcaaacga gaatccatca ccccgaacgg aaggttttgc gaaacgttag gatctgttcc
     1741 catccgaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
     1801 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
     1861 aataaagtta gtgacataac tacggtatgt ataattcacc agcgtgccgg gtggacaaat
     1921 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
     1981 attggtgcta ttaaagacat ttgagagatc atagtgttca tcttctgcta cgccagtact
     2041 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca ttgccaataa
     2101 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
     2161 cgaaataaca gtgacggctt gctgtaagct ttgctttggc aatacatagt gcgcaatgca
     2221 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
     2281 taaataaact tgcccgtcat cacccaccat actggttatt ccggtactgt tttcccgtac
     2341 cagtgatcca aacggcaccg gttttccgcc ctgggtaacg gtaatgagcg cccgcacgcc
     2401 tatgcgggta tcaaaattgg cacgaaccaa cgcgccttgc gtcggcacaa cgctgctaat
     2461 atttttttca acatcgatgg aattccccat cgtattggta tcaagcgcga tacggttata
     2521 ccgataaacc gtggcatacg gcatcaccgc atagccgcgc cagtcggtta aaatgccagt
     2581 ttgattttca atgcgtacac cgcctgcgcc aggcgcttta atcaaaacat tggtatcccc
     2641 taaaggctgg ctcagtgtta tgccattttc atgcccgacc acaccgccag acagctgcca
     2701 gttaacgtca tgttgatcgc ggtcgtagtt atacccgacc cccagcgtac cgtaagtggc
     2761 ctgccagttt gccgtggcgc tgcccgtgta cccattattc gaggtatcgc cctggcttac
     2821 gtgataactc aggttgtggc cttccagtaa ggtcccacca acgcctgcca gccagctatt
     2881 ttgcccgttg ctgttacggt tagcgttcaa ggaggcataa gcgcggtcga gcgcattttc
     2941 ccgggtgtag cgacgtttgg tcaaaacatt gaaaggcact gaaacattaa gtccgacaat
     3001 atgttcgtta tcggggatcc ctacagattc attccacgaa aatgagagtg aataactgat
     3061 gccaacccag ctgctggtat accccacctg ataccaggta tctgaatccg aagtattcca
     3121 gtatttttga tgggtaccag agatataaag cgagccaaag tcattaagtg attgtgaaat
     3181 atttaactgc aaacggtctt tgcggctaaa gcgtaaattg tggtaattca cgattatcgg
     3241 ttcatcgcga tgctcaccgt cgtaatcgta atcatattcg tacccctcca ttcgacgata
     3301 cgcaacatca tcaagggtat aaaaaccttg tgtcgaatag cggtaaccca ttaactgaaa
     3361 attggtgccg aaggtgtaca tcgatttcgc atagaggaag cgaatggaat ccccctcatg
     3421 acgactgtcg tcggctaact ggctgcgcgc atgggtcaca tccagcgaca ctgcgcccca
     3481 gttcccgagg ttgcgcccca gccctaataa aaaggcggtg taattggcag ataattgcgt
     3541 cccgccgtag gcagtaaatt cctgtggtaa accaccgagt gccgtgccct gaaaaaagaa
     3601 aggtgatgat tgctgactat taccgctacg aaaatcgccc gccgtcaggt caaatttgaa
     3661 acgcccttcg cgttgtaaaa tcggcactgt tgaatacgga attgtgtaat tctgccatcg
     3721 cgctcgtcga tggtgacatc cagatcgcca tttgaagatg tcgggtgcaa atcagtaatt
     3781 tcaaaagcac cgggggaaac atagctttga tagataataa aaccattttg ccgaatcgtt
     3841 agctgggccg ccgtacgggc aatcccacgt accgttgggg caaacccttg ctggctatct
     3901 ggatacatat tatcggaaga ataaagccgt acacca
//
LOCUS       DAZRVY010000242         3908 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZRVY010000242 DAZRVY010000000
VERSION     DAZRVY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.245"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHXOX_004239"
     CDS             24..590
                     /locus_tag="ACHXOX_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR7007544.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHXOX_004240"
     CDS             594..1169
                     /locus_tag="ACHXOX_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR7007545.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHXOX_004241"
     CDS             complement(1211..2263)
                     /locus_tag="ACHXOX_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR7007546.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRCRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHXOX_004242"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHXOX_004242"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR7007547.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHXOX_004243"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHXOX_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          972 a          987 c          951 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcatcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZRVY010000243         3834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZRVY010000243 DAZRVY010000000
VERSION     DAZRVY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.246"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHXOX_004244"
     CDS             complement(<1..266)
                     /locus_tag="ACHXOX_004244"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR7007548.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHXOX_004245"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHXOX_004245"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR7007549.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHXOX_004246"
                     /pseudo
     CDS             complement(726..998)
                     /locus_tag="ACHXOX_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHXOX_004247"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHXOX_004247"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR7007550.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3734)
                     /locus_tag="ACHXOX_004248"
     CDS             complement(2694..3734)
                     /locus_tag="ACHXOX_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR7007551.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         1000 a          932 c          916 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt acagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atga
//
LOCUS       DAZRVY010000244         3827 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZRVY010000244 DAZRVY010000000
VERSION     DAZRVY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.247"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHXOX_004249"
     CDS             <1..196
                     /locus_tag="ACHXOX_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007552.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHXOX_004250"
     CDS             207..374
                     /locus_tag="ACHXOX_004250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007553.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHXOX_004251"
     CDS             371..715
                     /locus_tag="ACHXOX_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007554.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXOX_004252"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXOX_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR7007555.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHXOX_004253"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHXOX_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHXOX_004254"
     CDS             1710..1988
                     /locus_tag="ACHXOX_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007556.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHXOX_004255"
     CDS             1990..3048
                     /locus_tag="ACHXOX_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR7007557.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHXOX_004256"
     CDS             3049..3429
                     /locus_tag="ACHXOX_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7007558.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>3827
                     /locus_tag="ACHXOX_004257"
     CDS             3426..>3827
                     /locus_tag="ACHXOX_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7007559.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          852 c         1046 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZRVY010000245         3798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZRVY010000245 DAZRVY010000000
VERSION     DAZRVY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.248"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHXOX_004258"
     CDS             <1..82
                     /locus_tag="ACHXOX_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007560.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHXOX_004259"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHXOX_004259"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR7007561.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHXOX_004260"
     CDS             1028..1486
                     /locus_tag="ACHXOX_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR7007562.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHXOX_004261"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHXOX_004261"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR7007563.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHXOX_004262"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHXOX_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR7007564.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="ACHXOX_004263"
     CDS             2692..3369
                     /locus_tag="ACHXOX_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR7007565.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHXOX_004264"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHXOX_004264"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR7007566.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          952 a          903 c          989 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccaag cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAZRVY010000246         3740 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZRVY010000246 DAZRVY010000000
VERSION     DAZRVY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.249"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            75..182
                     /locus_tag="ACHXOX_004265"
     CDS             75..182
                     /locus_tag="ACHXOX_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7007567.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(269..1948)
                     /gene="bcsG"
                     /locus_tag="ACHXOX_004266"
     CDS             complement(269..1948)
                     /gene="bcsG"
                     /locus_tag="ACHXOX_004266"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR7007568.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1945..2136)
                     /gene="bcsF"
                     /locus_tag="ACHXOX_004267"
     CDS             complement(1945..2136)
                     /gene="bcsF"
                     /locus_tag="ACHXOX_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR7007569.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2133..3704)
                     /gene="bcsE"
                     /locus_tag="ACHXOX_004268"
     CDS             complement(2133..3704)
                     /gene="bcsE"
                     /locus_tag="ACHXOX_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR7007570.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT          956 a          940 c          956 g          888 t
ORIGIN      
        1 cacgggggct aatcttgaac ccaaacaact caagaatagc cgcgaaacgt tggcattaca
       61 acacaggcgg ctatatgacg ctcgcagaac tgggcatggc cttctggcat gatttagcgg
      121 ctccggtcat tgctggcatt cttgccagta tgatcgtgaa ctggctgaat aagcggaagt
      181 aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag
      241 aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag tcgccgccgt
      301 tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag ttctcggaga
      361 ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg tcttcggtga
      421 aaatcttgcc atcaagaacg cgaaccacca gatcggagat agctaggaag ctgctcggtt
      481 ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat ttcaccccaa
      541 cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc tgcattctgt
      601 cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta cgacccgatt
      661 tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc cgcgctttgt
      721 aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga agcgtgttgt
      781 agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag cggttaagca
      841 cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc ggcagatttg
      901 tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc aaaaaaccgc
      961 cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc gaaaggttat
     1021 caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa gtctgcccgc
     1081 agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg gagttaaagt
     1141 tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg gcggcttcta
     1201 tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc tgagcatcag
     1261 cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag aaattattca
     1321 gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata tcacccacta
     1381 ccgatgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc gttgttacag
     1441 tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg gtaagtacgt
     1501 tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt gacaggaata
     1561 accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata aagcgtgtga
     1621 caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg ctcattatgc
     1681 tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc ggcagggcaa
     1741 tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga aacgtggcaa
     1801 acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg ccgaacttaa
     1861 ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg gaagaaggca
     1921 tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt ccgtgcggcg
     1981 taacgtcccg gccggtttaa cataacgagg tttagcaaag aataaacgta aggtgtcgcg
     2041 aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca gtgcgcaaac
     2101 gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac agcatcatct
     2161 aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc ggcattgatg
     2221 accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg ggcaagcaag
     2281 cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat acggtttgag
     2341 aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag atcgttaatc
     2401 cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat atcgccggta
     2461 cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac cggacgtagg
     2521 gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat gttgttgacg
     2581 gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg ggtcattgac
     2641 agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg cacgctttcg
     2701 atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt tgcaccgcag
     2761 gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg cacgaggatt
     2821 ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag tggctcgatt
     2881 tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg ggcttcattg
     2941 aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg cgcaccttcc
     3001 agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat ctccgcctct
     3061 tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg ctgacgggcg
     3121 ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc accctgaaaa
     3181 cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga aaattgttta
     3241 tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct gctccattta
     3301 ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca tgcgttattt
     3361 gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa atcacgggcc
     3421 aaatagtata gacctttttc atgatttagc attgaaaata attttatttt ttccggccct
     3481 tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat gaccgcgacg
     3541 tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc atcttcatgg
     3601 cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc atcccataat
     3661 gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc attttgtctg
     3721 attatcagtt tactagcgaa
//
LOCUS       DAZRVY010000247         3733 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZRVY010000247 DAZRVY010000000
VERSION     DAZRVY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.250"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHXOX_004269"
     CDS             complement(<1..181)
                     /locus_tag="ACHXOX_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR7007571.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHXOX_004270"
     CDS             425..682
                     /locus_tag="ACHXOX_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR7007572.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXOX_004271"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXOX_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR7007573.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXOX_004272"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXOX_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR7007574.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHXOX_004273"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHXOX_004273"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR7007575.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3733)
                     /locus_tag="ACHXOX_004274"
     CDS             complement(3307..>3733)
                     /locus_tag="ACHXOX_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR7007576.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          952 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aat
//
LOCUS       DAZRVY010000248         3641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZRVY010000248 DAZRVY010000000
VERSION     DAZRVY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.251"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(34..78)
                     /locus_tag="ACHXOX_004275"
     CDS             complement(34..78)
                     /locus_tag="ACHXOX_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007577.1"
                     /translation="MKRNDKILHYEGLN"
     gene            197..1921
                     /gene="ilvI"
                     /locus_tag="ACHXOX_004276"
     CDS             197..1921
                     /gene="ilvI"
                     /locus_tag="ACHXOX_004276"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR7007578.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1924..2415
                     /gene="ilvN"
                     /locus_tag="ACHXOX_004277"
     CDS             1924..2415
                     /gene="ilvN"
                     /locus_tag="ACHXOX_004277"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR7007579.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2427..2513
                     /locus_tag="ACHXOX_004278"
     CDS             2427..2513
                     /locus_tag="ACHXOX_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR7007580.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2595..3599
                     /gene="cra"
                     /locus_tag="ACHXOX_004279"
     CDS             2595..3599
                     /gene="cra"
                     /locus_tag="ACHXOX_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR7007581.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          847 a          868 c         1010 g          916 t
ORIGIN      
        1 tgctgtaagt tgtgggattc agccgattta ttatcaattt aatccttcgt aatggaggat
       61 tttatcgttt cttttcacct ttcctcctgt ttattcttat taccctgcgt ttatgtctct
      121 ggctgccaat tgcttaagca agatcggacg gttaatgtgt tttacacatt ttttccgtca
      181 aacagtgagg caggccatgg agatgttgtc tggagccgag atggtcgtcc gatcgcttat
      241 cgatcagggc gttaaacaag tattcggtta tcccggaggc gcagtccttg atatttacga
      301 tgcattgcat accgtgggtg gtatcgatca tgttttagtt cgtcatgagc aggcggcagt
      361 gcatatggcc gatggtctgg cgcgcgcgac cggggaagtc ggcgtcgtgc tggtaacgtc
      421 gggtccaggg gcgaccaatg cgattactgg catcgccacc gcttatatgg attccattcc
      481 attagttgtc ctttccgggc aggtagcgac ctcgttgata ggttacgatg cctttcagga
      541 gtgcgacatg gtggggattt cgcgaccagt ggttaaacac agttttctgg ttaagcaaac
      601 ggaagacatt ccgcaggtgc tgaaaaaggc tttctggctg gcggcaagtg gtcgtcctgg
      661 accagtagtc gttgatttac cgaaagatat tcttaatccg gcgaacaaat taccctatgt
      721 ctggccggag tcggtcagta tgcgttctta caatcccact actaccggac ataaagggca
      781 aattaagcgc gctctgcaaa cgctggtagc ggcaaaaaaa ccggttgtct acgtaggcgg
      841 tggggcaatc acggcgggct gccatcagca gttgaaagaa acggtggagg cgttgaatct
      901 gcccgttgtt tcctcattga tggggctggg agcgtttccg gcaacgcatc gtcaggcact
      961 gggcatgctg ggaatgcacg gtacctacga agccaatatg acgatgcata acgcggatgt
     1021 gattttcgcc gtcggggtac gatttgatga ccgaacgacg aacaatctgg caaagtactg
     1081 cccaaatgcc actgttctgc atatcgatat tgatcctact tccatttcta aaaccgtgac
     1141 tgcggatatc ccgattgtgg gggatgctcg ccaggtcctc gaacaaatgc ttgaactctt
     1201 gtcgcaagaa tccgcccatc aaccactgga tgagatccgc gactggtggc agcaaattga
     1261 acagtggcgc gctcgtcagt gcctgaaata tgacactcac agtgaaaaga ttaaaccgca
     1321 ggcggtgatc gagactcttt ggcggttgac gaagggagac gcttacgtga cgtccgatgt
     1381 cgggcagcac cagatgtttg ctgcacttta ttatccattc gacaaaccgc gtcgctggat
     1441 caattccggt ggcctcggca cgatgggttt tggtttacct gcggcactgg gcgtcaaaat
     1501 ggcgttgcca gaagaaaccg tggtttgcgt cactggcgac ggcagtattc agatgaacat
     1561 ccaggaactg tctaccgcgt tgcaatacga gttgcccgta ctggtggtga atctcaataa
     1621 ccgctatctg gggatggtga agcagtggca ggacatgatc tattccggcc gtcattcaca
     1681 atcttatatg caatcgctac ccgatttcgt ccgtctggcg gaagcctatg gtcacgtcgg
     1741 gatccagatt tctcatccgc atgagctgga aagcaaactt agcgaggcgc tggaacaggt
     1801 gcacaataat cgcctggtgt ttgttgatgt taccgtcgat ggcagcgagc acgtctaccc
     1861 gatgcagatt cgcgggggcg gaatggatga aatgtggtta agcaaaacgg agagaacctg
     1921 attatgcgcc ggatattatc agtcttactc gaaaatgaat caggcgcgtt atcccgcgtg
     1981 attggccttt tttcccagcg tggctacaac attgaaagcc tgaccgttgc gtcaaccgat
     2041 gatccgacat tatcgcgtat gaccatccag accgtgggcg atgaaaaagt acttgagcag
     2101 atcgaaaagc aattacacaa gctggtcgat gtcttgcgcg tgagtgagtt ggggcagggc
     2161 gcgcatgttg agcgggaaat catgctggtg aaaattcagg ccagcggtta cgggcgtgac
     2221 gaagtgaaac gtaatacgga aatattccgt gggcaaatta tcgatgtcac accctcgctt
     2281 tataccgttc aattagcagg caccagcgat aagcttgatg catttttagc atcgattcgc
     2341 gaggtggcga aaattgtgga agttgctcgt tctggtgtgg tcggactttc gcgcggcgat
     2401 aaaataatgc gttgagaatg atctgaatgc gcaatttgca gcccaacatg tcacgttggg
     2461 ctttttttgc gaaatcagtg ggaacctgga ataaaagcag ttgccgcagt taattttatg
     2521 cgcttagatg ttaatgaatt taacccatac cagtacaatg gctatggttt ttacatttta
     2581 cgcaaggggc aattgtgaaa ctggatgaaa tcgctcggct ggcgggagtg tcgcggacca
     2641 ctgcaagcta tgttattaac ggcaaagcga agcaataccg tgtgagcgac aaaaccgttg
     2701 aaaaagtcat ggctgtggtg cgtgagcaca attaccaccc gaacgccgtg gcagctgggc
     2761 ttcgtgctgg acgcacacgt tctattggtc ttgtgatccc cgatctggag aacaccagct
     2821 atacccgcat cgctaactat cttgaacgcc aggcgcggca acggggttat caactgctaa
     2881 ttgcctgctc agaagatcag ccagacaacg aaatgcgctg tattgagcac cttttacagc
     2941 gtcaggtcga tgccattatt gtttcgacgt cgttgcctcc tgagcatcct ttttatcaac
     3001 gctgggctaa cgacccgttc ccgattgtcg cgctggaccg cgccctcgat cgtgaacact
     3061 tcaccagcgt ggttggtgcc gatcaggatg atgccgaaat gctggcggaa gagttacgta
     3121 agtttcccgc cgagacggtg ctttatcttg gcgcgctgcc ggagctttct gtcagcttcc
     3181 tgcgtgaaca aggtttccgt actgcctgga aagatgatcc gcgcgaagtg catttcctgt
     3241 atgccaacag ctatgagcgg gaggtggctg cccagttatt cgaaaaatgg ctggaaacgc
     3301 atccgatgcc gcaggcgctg ttcacaacgt cgtttgcgtt gttgcaaggg gtgatggatg
     3361 tcacgctacg tcgcgatggt aaactgcctt ctgacctggc aattgccacc tttggcgata
     3421 acgaactgct cgacttctta cagtgcccgg tgctggcagt ggctcaacgt caccgcgatg
     3481 tcgcagagcg tgtgctggag attgtcctgg caagcctgga cgaaccgcgt aagccaaaac
     3541 ctggtttaac gcgcattaaa cgtaatctct atcgccgcgg tgtgctcagc cgtagctaag
     3601 ccgcgaacaa aaatacgcgc caggtgaatt tccctctggc g
//
LOCUS       DAZRVY010000249         3627 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZRVY010000249 DAZRVY010000000
VERSION     DAZRVY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.252"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHXOX_004280"
     CDS             complement(89..652)
                     /locus_tag="ACHXOX_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR7007582.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHXOX_004281"
     CDS             complement(756..974)
                     /locus_tag="ACHXOX_004281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007583.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHXOX_004282"
     CDS             complement(1133..1519)
                     /locus_tag="ACHXOX_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR7007584.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHXOX_004283"
     CDS             complement(1509..1880)
                     /locus_tag="ACHXOX_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7007585.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHXOX_004284"
     CDS             complement(1880..2137)
                     /locus_tag="ACHXOX_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007586.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHXOX_004285"
     CDS             complement(2134..3525)
                     /locus_tag="ACHXOX_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR7007587.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHXOX_004286"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHXOX_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7007588.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZRVY010000250         3560 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZRVY010000250 DAZRVY010000000
VERSION     DAZRVY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.253"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHXOX_004287"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHXOX_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR7007589.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHXOX_004288"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHXOX_004288"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR7007590.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHXOX_004289"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHXOX_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR7007591.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT          968 a          930 c          835 g          827 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcggatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat
//
LOCUS       DAZRVY010000251         3559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZRVY010000251 DAZRVY010000000
VERSION     DAZRVY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.254"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHXOX_004290"
     CDS             129..1376
                     /locus_tag="ACHXOX_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR7007592.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHXOX_004291"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHXOX_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR7007593.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHXOX_004292"
     CDS             complement(2228..2572)
                     /locus_tag="ACHXOX_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007594.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHXOX_004293"
     CDS             2814..3416
                     /locus_tag="ACHXOX_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007595.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          887 a          714 c          816 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttaaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAZRVY010000252         3543 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZRVY010000252 DAZRVY010000000
VERSION     DAZRVY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.255"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHXOX_004294"
     CDS             complement(<1..140)
                     /locus_tag="ACHXOX_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR7007596.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXOX_004295"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXOX_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR7007597.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHXOX_004296"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHXOX_004296"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR7007598.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHXOX_004297"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHXOX_004297"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR7007599.1"
                     /translation="QAYFDQLDRIRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          981 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaatgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAZRVY010000253         3533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZRVY010000253 DAZRVY010000000
VERSION     DAZRVY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.256"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..638)
                     /locus_tag="ACHXOX_004298"
     CDS             complement(<1..638)
                     /locus_tag="ACHXOX_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7007600.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
     gene            complement(645..953)
                     /locus_tag="ACHXOX_004299"
                     /pseudo
     CDS             complement(645..953)
                     /locus_tag="ACHXOX_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1332..1844)
                     /locus_tag="ACHXOX_004300"
                     /pseudo
     CDS             complement(1332..1844)
                     /locus_tag="ACHXOX_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(1857..2279)
                     /locus_tag="ACHXOX_004301"
     CDS             complement(1857..2279)
                     /locus_tag="ACHXOX_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR7007601.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2276..2518)
                     /locus_tag="ACHXOX_004302"
     CDS             complement(2276..2518)
                     /locus_tag="ACHXOX_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7007602.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2615..3034
                     /locus_tag="ACHXOX_004303"
     CDS             2615..3034
                     /locus_tag="ACHXOX_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7007603.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3340..3495
                     /locus_tag="ACHXOX_004304"
     CDS             3340..3495
                     /locus_tag="ACHXOX_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR7007604.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          893 a          928 c          794 g          918 t
ORIGIN      
        1 agtgtattca tcgctgcatg gttaatattt gtcagcatcc ccacaccgcg taacgatgcc
       61 gtccggcgat caacaatctg gttcagtacc acctgctcgt ttttcgtatc ccgctgcatg
      121 ccaatttcat ccaggaccag caggtcaaca ccacaaagct cctgtaaaaa tttttcaccg
      181 gatttgccgt tgtcgtagct ctcatgcaac acgctcatga catcggacac ggtgacgata
      241 atcacgctac gccccttcgc catcagccgg ttgccaatcg cgtctgcaag atgatttttt
      301 ccggtgccgg gcttaccgct gaacacaaaa ttcgtacagc ctgtcatcag ttcgtcagct
      361 atggatttcg cctgactcag tgcatgtcgc tgaccgtcgt tctgcgcccg gtaattcgca
      421 aacgaacact tccggtgcaa cggctggatg ccggagcggt tcaggatttt ttccacccgc
      481 aactgacgat tcaggcggtt gatctcctcg caacgtttct ggccttcagc aagttgccac
      541 tcgcgccact ccgcaaccgt tctgaatggg gcggttacat gtggtggggt cagtctgcgg
      601 atacgctcca gaacgcctcc tgtcgcaata tttttcatgg tctgttaccc cctgaagcct
      661 ggcgggatcg cactgtccgg caacgagacg gtgttaacct gtcggagcaa cgtctcaggc
      721 cgaacacctt tcggcgcgaa cagaccctgg tattcattgg cgatgctgtg tcgaatcacc
      781 tgctcaggtg taaaaccctg ctgacggaat ttttccagtt cccgtatcgc cccgttagcg
      841 ccctgctccg ttcgaatcgg ttttcgcaat gcctgcctga actggaccca ctcatgccag
      901 agtgtttctg gcaaccagtc gggcagatca actgacaacg ggtcaaattt tttctggcga
      961 acagccccac cgataaccag aattccctgc tcggtgtgcc ttgcgatatc actgcgccgc
     1021 aagacacata tcccggtttc aacagcaccg attttgagca gtgatttctg tcgcggagaa
     1081 tttcggtcaa gctcgctccc acaccggaca ccgttcctgt cagtcacgac gatatcaatc
     1141 cgccctccgc aaccgtcacc acgctctggc accggatact cacgacagac atccaggcca
     1201 gcagcctgca acgcaaccac cgcagaatta caaaattccg ctgcagtacc gccagataat
     1261 ctcccctcaa gcactccagc cacaaaatcc cgaaactccc ttccggaagg gaagggtttg
     1321 ggatgggtta gatctgtttt tagatcttta tctgtatctt tattagttgc ctttgtgttg
     1381 gcatcatgtt cgaacatcac tccaacatct gtttgaacac ctgttaaatt tctctcttgt
     1441 tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc ttttcctgcg
     1501 gctgattttt tggttaattt ttctctgaca gatgccagat cttcctcaat ccgaagatgc
     1561 atccattcgt cgccgttatc acaaaaaaac tcccgcaagg atggttcaac atcaacccat
     1621 cgctcgttag tcagacgggc aatttttgcc aacctgtttt tgggtattgg ctttcctgtt
     1681 tgccagtaat tgaacatcag caacaaatac gcgccgtgct cctctgctga caaatgcatg
     1741 gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc cataattgcc
     1801 ccgtatgatg ctgcccggtt gcttagaata agcacaaaca gcatggaaac ttttgcttaa
     1861 tgaacaatga cagaatcgtc ggaagacccg ccgccgctga aatgcgcttt ccggtaaacg
     1921 gcctggactg catcatcatg cgcatcaatt gccgtactca acgcttcctg cgccgccagt
     1981 aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc gcgcggaaga
     2041 attgccagaa ccgcagggat cagtttctga attttttccc tttgcgcttt cgtttcacct
     2101 ttcaaccaac ggtgatagat attctgctga ttgttccagt ccttgcctgg tacaaggggc
     2161 aattcgccgc ccccctggcg cagatattct tcagtaattg cgttagcgac ccacgcctgc
     2221 cctttttcgg ctgccagggc taacaacact gattcgatgt gctcatgcct gattttcatg
     2281 aatcaaccgc tcctatgctg ttttcgctat gcttaccgtc tggggggaat acatcgtcaa
     2341 gtccacaatg agcgccaagc cgattaaggg tagaaacaat ttttctgcac tcctctagtc
     2401 caggggtacg aaaatttgct tcgtaatttg ccagtcggct ttgtatccac cctaactgaa
     2461 cagcaagttg tctttgagac agcccaagct gttttcgata tgttgaaatt ttgttcattg
     2521 aaaacctccg atgacaattt taaacacacc ttgtgttata tagccaagct gttttgtgtt
     2581 ttatgtaaat cacgattcgt gatacaagga tgcaatggaa aaagaaaacg aaaaaattgc
     2641 cgctagtagg ctcaatgaca aaattgcaat gcgtcttaaa gagcgcaggc agaagcttgg
     2701 tttatctcaa ggaaaacttg ctgaaatctg cggatggacg caatcgcgta taggtaacta
     2761 tgaggcgggc agcagaaatg ttggagtaca tgacgctgtc gtattgggaa aggcacttgg
     2821 catatctcct cctgagctcc tctttggaga acaggaatct tctgaattgt ggttaaatga
     2881 atcccaacga aaacttcttg agttgtttaa ccagctaccg ggctcagaac aacaacgaat
     2941 gattgagcta tttgaagtcc ggctaaaaga aatcgatgag tatgtagaaa aatatttgag
     3001 aggcaggatt aaagataatc ccccaccgga ataatgatct tgctatcaca gtaatatgcc
     3061 aatcagcccg ctatcagcgg gcttttttgt agcatcatca tatgacactc accataaaac
     3121 acattgcgtg ttgacacaag aaaacaaatt gtgtttaata agcatatcca aacaacgccc
     3181 caccagagaa cggcaggaca atacctcgag ttatccagtc actgaacagg gctaagtagc
     3241 cagcctgagg catacgaaca tgacggcagt tgttgattga tacaaagcgc agtagataaa
     3301 acgttccgcc acccggcgtt aaggggaaat ggggtcaaca tgaatactat cgatcttggc
     3361 aacaacgaat ctctggtatg tggcgtgttt cccaatcaag acggcacatt caccgccatg
     3421 acgtatacca aaagcaaaac gtttaaaacc gaagctggcg cacgtcgttg gttgggaaga
     3481 cattcaggtg agtaaaatga acgagacaga attaaaacac gttatcgctc tac
//
LOCUS       DAZRVY010000254         3322 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZRVY010000254 DAZRVY010000000
VERSION     DAZRVY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.257"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHXOX_004305"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHXOX_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR7007605.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHXOX_004306"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHXOX_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR7007606.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHXOX_004307"
     CDS             complement(1250..2824)
                     /locus_tag="ACHXOX_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR7007607.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHXOX_004308"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHXOX_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZRVY010000255         3309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZRVY010000255 DAZRVY010000000
VERSION     DAZRVY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.258"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHXOX_004309"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHXOX_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHXOX_004310"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHXOX_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR7007608.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHXOX_004311"
     CDS             2354..2758
                     /locus_tag="ACHXOX_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR7007609.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="ACHXOX_004312"
     CDS             2811..2921
                     /locus_tag="ACHXOX_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007610.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="ACHXOX_004313"
     CDS             3157..3201
                     /locus_tag="ACHXOX_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007611.1"
                     /translation="MFSKLAPSSIKAMF"
BASE COUNT         1000 a          675 c          678 g          956 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccatt
//
LOCUS       DAZRVY010000256         3303 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZRVY010000256 DAZRVY010000000
VERSION     DAZRVY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.259"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            186..902
                     /gene="nanC"
                     /locus_tag="ACHXOX_004314"
     CDS             186..902
                     /gene="nanC"
                     /locus_tag="ACHXOX_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR7007612.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            922..2028
                     /gene="nanM"
                     /locus_tag="ACHXOX_004315"
     CDS             922..2028
                     /gene="nanM"
                     /locus_tag="ACHXOX_004315"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR7007613.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2093..3073
                     /locus_tag="ACHXOX_004316"
     CDS             2093..3073
                     /locus_tag="ACHXOX_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR7007614.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3183..3303
                     /locus_tag="ACHXOX_004317"
                     /pseudo
     CDS             3183..3303
                     /locus_tag="ACHXOX_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          982 a          695 c          772 g          854 t
ORIGIN      
        1 gattctctgt ttatttaaat aatgtggaaa gagaattttc acaggagacc ttatacaaaa
       61 atataaaata agctacccgt tgccaaagac actataagcc tggcaaaaaa tattacacaa
      121 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
      181 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtcaccat
      241 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagcgga agccatgcct
      301 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
      361 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
      421 aggttgaagt taattacgcg attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
      481 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
      541 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
      601 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
      661 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
      721 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
      781 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
      841 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
      901 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
      961 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     1021 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     1081 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     1141 ccgagagatc aagcaacctc tgcatttatt gatggcaatc tatatgtgtt tggcggcatt
     1201 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     1261 accaatagct gggttaaatt gatgtcgctc gcgccgatgg gcatggcggg tcatgtgact
     1321 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     1381 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     1441 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     1501 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     1561 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     1621 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     1681 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     1741 cttatatttg ccggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     1801 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     1861 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     1921 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     1981 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     2041 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     2101 aataatatcg cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     2161 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     2221 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     2281 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     2341 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     2401 actgcccttc attcctaata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     2461 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     2521 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     2581 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     2641 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     2701 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccatggtt
     2761 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     2821 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     2881 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     2941 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     3001 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     3061 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     3121 acaacaaaaa acaaataaga tcaaggtaat atctcttcac ttgcgggctc gaacagcagg
     3181 caattgccaa tataataatc tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt
     3241 tattgcttac agattagccg gatgccttaa tagacatccg cgccttttcg attatggctc
     3301 gga
//
LOCUS       DAZRVY010000257         3299 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZRVY010000257 DAZRVY010000000
VERSION     DAZRVY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.260"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXOX_004318"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXOX_004318"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR7007615.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXOX_004319"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXOX_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR7007616.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXOX_004320"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXOX_004320"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR7007617.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXOX_004321"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXOX_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR7007618.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          777 a          874 c          783 g          865 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctct
//
LOCUS       DAZRVY010000258         3236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZRVY010000258 DAZRVY010000000
VERSION     DAZRVY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.261"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHXOX_004322"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHXOX_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR7007619.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHXOX_004323"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHXOX_004323"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR7007620.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHXOX_004324"
     CDS             1253..1522
                     /locus_tag="ACHXOX_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR7007621.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHXOX_004325"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHXOX_004325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR7007622.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHXOX_004326"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHXOX_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR7007623.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          826 a          781 c          786 g          843 t
ORIGIN      
        1 aaggccatcc tgacggatgg cctttttgca ttggcgcaga aaaaaatgcc ggatgcgaca
       61 ctggcgcgtc ttatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      361 tcttcgtctg gtttttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      541 tttttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaaca aatgcatctt
      661 tatgatcgct gaaatcaacc gcatggccgc cagccagacg cataaatgcc attacccggc
      721 gttctcctgc ttgcagatat tctagtaata aaggctcaat agcacggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcatgga cccacacgac aggcgcatct ttgcgctgat
      841 gattaagccg ggaagctaaa acatggggaa tgtaaggcgt atcgcacgga caaaacaaaa
      901 accactcacc agcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatgacgat
     1021 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatca ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgttg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1501 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg acgctctgtt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 agcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gacggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcaca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagccacgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agatttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gcggatgcaa ctggaaacta
     2341 ttattgaagc ttatgaagaa tttggcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggctg cgacaaacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttacaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgtcc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tcactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt caaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggcgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggtatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac agtgaaatat ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAZRVY010000259         3216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZRVY010000259 DAZRVY010000000
VERSION     DAZRVY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.262"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHXOX_004327"
     CDS             <1..495
                     /locus_tag="ACHXOX_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7007624.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHXOX_004328"
     CDS             525..3041
                     /locus_tag="ACHXOX_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7007625.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="ACHXOX_004329"
     CDS             3052..>3216
                     /locus_tag="ACHXOX_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7007626.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAZRVY010000260         3048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZRVY010000260 DAZRVY010000000
VERSION     DAZRVY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.263"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHXOX_004330"
     CDS             complement(<1..271)
                     /locus_tag="ACHXOX_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR7007627.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..860)
                     /locus_tag="ACHXOX_004331"
     CDS             complement(315..860)
                     /locus_tag="ACHXOX_004331"
                     /inference="COORDINATES: protein motif:HMM:NF019114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR7007628.1"
                     /translation="MDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVL
                     KPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTA
                     VRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIEDGNKQEE
                     INEDTEESTTDDTTAEDTENE"
     gene            896..1459
                     /locus_tag="ACHXOX_004332"
     CDS             896..1459
                     /locus_tag="ACHXOX_004332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007629.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1605..1907)
                     /locus_tag="ACHXOX_004333"
     CDS             complement(1605..1907)
                     /locus_tag="ACHXOX_004333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007630.1"
                     /translation="MEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFYVTRYLG
                     KMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
     gene            complement(2127..2567)
                     /locus_tag="ACHXOX_004334"
     CDS             complement(2127..2567)
                     /locus_tag="ACHXOX_004334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007631.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
BASE COUNT          938 a          605 c          499 g         1006 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccat cctctattac ttccatgtca
      421 tcgggtaact caaattccat tgagcctaat tggtataatg ttttatcaat atgcgttctt
      481 aatacatcgc gtaaatcctt tgcatctgat tgtgcaaata attcaagata agtttttgat
      541 ggtattgcac gcactgctgt tttgacctgg aatagatatt ccgttagtac ctgttctata
      601 tattcagtac tgaccaccag cccattcttt tctgctaatt ctaattctgc taactgccgt
      661 tctgctgaaa gcttcttcaa acgctcttgt tcaatttgtt cttttgtatt agtatcacgc
      721 aacggcttta gtacattatc tacaatccaa cttcttgcgt ttgaatcagt gtctgtcggc
      781 attccctttt ctacccaagt tcgaactgta gattcatcgt atccgtactg tctcgctaat
      841 gaccttaatg agatatccat atttgttcct ttttatttta tttatagggt ttacaatgtc
      901 agaccgtaac aatgattttt ctctgttagt aaaattgctg aacggatttt tgaagaaaga
      961 tgagataaaa aaaagactat attctttcgt gaaaaaagaa actaatgatt gggaaaagtg
     1021 gctgcaaata gaatttgctc attatctaac ttcaaaaagg aagtacgagc tacatcgtga
     1081 agttacgata tacctcgatc caatagatta tcctgatagt aaacttgcta aagtagattt
     1141 gatactgcgt gaaaaatcca caccctctga tgattatatt tttattgagt ttaaatgtac
     1201 taaaggtgta gtaccattag ttaggggctt aaatgctgac aaaaataaaa ttagtgctat
     1261 aagacagtgt aactacaaaa tgcgtaatta tcttggtgtc ggatttcatc ttttttgtga
     1321 accagatgat atcgagttta tggaaaagta cgtaactgaa gaacttaaag gctcttacgc
     1381 cgtgtttaaa ttatgcgagt gtccagtaag atataattgc caatgtgaat ttaatgaaat
     1441 tggtgtggta cttttttaaa tgcggtgcgg gtatgatcat tttgttcaca cacaattgat
     1501 taaacccgct gccgaaaacc ctcgatagta ccgccagtat ggagggaccc attgaatgca
     1561 atggttaata tactttgtaa gctatataca atgaatcatg agtatcaaag ccgcttacat
     1621 ttaatatcat cgtacaaagt tccttctttg tttagatatc tcgaaccgta attatatcca
     1681 tcatcaaatg cttcattggt tattaccagt tccctgtaaa atttctcatc aggtgctgtt
     1741 tcataatgaa atgcaccata aggcggcggc attttaccga gatatcttgt aacgtagaaa
     1801 ctcatgtcag tactaaatga atcgttatga atatcccagc atttaaagtc tggaatagct
     1861 gcacttgttg ttaaaggtaa taataatagc aatatcaatt tttccataag agataccaat
     1921 gcaaatgatt taattgcatg ataacaccta caaagtctca ataccaatta acaagcgata
     1981 tggctgattt tgtttcaatt acgtaaaata atttaaaata tcgttaatta aaacaattgt
     2041 gatgaaatgc tcaccttgta atttgttgcg tatttgatga aagtacttac ccttcatcaa
     2101 atacacattt ctttagtact tgccctttac ttgataagca aactatagaa ttttcttgtc
     2161 tgctcaactg caacgacaaa acagccaatt gcaaaacatg ctctcaaccc accatccccg
     2221 ccgtgtaatg acattgcggt agtgaatata aaattagtca ccaagaaagt aatagccata
     2281 tacaagatga aatatagaaa tgagaaaatg cggcaaagta tattactgct tttaatatca
     2341 aatatctttg caaatacttt cgttagtggt gaaaaaagca tagcaataaa accaggaact
     2401 acagttagta agaatatcat tactaacatt aaaggtactt taacgtattc ataaccacta
     2461 acacgagtta aaataagtac tccgacgata cctgttatta agccaccgat tagtagttct
     2521 ttagttcctg caaacatttc agagatatta tcgactgtaa ttttcattct actaactatt
     2581 ccttcataaa aagttcactc cgatcttatc accgcataaa gaaaaagccc tgtagattct
     2641 caccatacag cctaagcact aaacttttcg gattacttcg atactacctg ccattgtcca
     2701 tcatgccgga tgatctcata ctggtactta ccatcacacc gtacatcaat acgtcctgta
     2761 ccattctcag tactatccat agtcaccata tcgcatgggt acttaccctt gattagtgac
     2821 tgtgcgaaat ccatagcgtt ctggttctca ttgtccgtgt actgtggctg gggtgcatcc
     2881 tcacctgcaa tgttgaagag taccccagcc agtactaaca aaatcaccat tacctttccc
     2941 atacgtaccc tatagattgt cgaggatatg ggatggtgaa ttgacccctt ctaaccaatc
     3001 gtgaacacta tcggtctgat gtggtactac cttacttaca tgctctct
//
LOCUS       DAZRVY010000261         3045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZRVY010000261 DAZRVY010000000
VERSION     DAZRVY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.264"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHXOX_004335"
     CDS             <1..172
                     /locus_tag="ACHXOX_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007632.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHXOX_004336"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHXOX_004336"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR7007633.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHXOX_004337"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHXOX_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHXOX_004338"
     CDS             2190..>3045
                     /locus_tag="ACHXOX_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR7007634.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          835 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccacgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZRVY010000262         2992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZRVY010000262 DAZRVY010000000
VERSION     DAZRVY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.265"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXOX_004339"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXOX_004339"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR7007635.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXOX_004340"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXOX_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR7007636.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHXOX_004341"
     CDS             2805..>2992
                     /locus_tag="ACHXOX_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR7007637.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZRVY010000263         2991 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZRVY010000263 DAZRVY010000000
VERSION     DAZRVY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.266"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(8..163)
                     /locus_tag="ACHXOX_004342"
     CDS             complement(8..163)
                     /locus_tag="ACHXOX_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR7007638.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(366..773)
                     /locus_tag="ACHXOX_004343"
     CDS             complement(366..773)
                     /locus_tag="ACHXOX_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7007639.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            850..1077
                     /locus_tag="ACHXOX_004344"
     CDS             850..1077
                     /locus_tag="ACHXOX_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR7007640.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1061..1482
                     /locus_tag="ACHXOX_004345"
                     /pseudo
     CDS             1061..1482
                     /locus_tag="ACHXOX_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1560..2347
                     /locus_tag="ACHXOX_004346"
                     /pseudo
     CDS             1560..2347
                     /locus_tag="ACHXOX_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2354..>2991
                     /locus_tag="ACHXOX_004347"
     CDS             2354..>2991
                     /locus_tag="ACHXOX_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7007641.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKT"
BASE COUNT          785 a          648 c          823 g          735 t
ORIGIN      
        1 gttcatttta ctcacctgaa tgtcttccca accaacgacg tgcgccagct tcggttttaa
       61 acgttttgct tttggtatac gtcatggcgg tgaatgtgcc gtcctggttg gggaacacgc
      121 cgtacaccag agattcgttg ttgccaagat cgatagtatt catgttgacc ccatttcccc
      181 ttaacgccgg ggtagcggaa ctgtttgctg agaacaccgt gcggtgtctt gatgaatgaa
      241 atttagaata acctaaggtg ggcggtcaag atttttgtgt agaaaaacct aagttttttg
      301 atgtaaaaaa cacaagtgtt tgaaagtttg tgctttttat tacagggtgt ggagaaaaaa
      361 ggggattatt tgtttgcgct tcttttgcga gctttgagta gttcttcaaa aagtttgttg
      421 aaattttcaa ctcgagcacg catctctgac aacagggcct tttgctctga ctcaggcagt
      481 gcgtcgaaca gttgaagtaa ctctttttga tcttctgtca gaatggctgg ctgattatcc
      541 gggatcggtt cgcctggttg tttatcttca tccccaaaaa gaagccaagt cggtgagcac
      601 tgaagcgctt ggctcagtgc gaataatctt ttccccgccg gctgtgtttc atctctttcc
      661 cattgagaaa ttgttacgtg agccactttg accagcttac ctaatgcggc ctgagacagt
      721 tttaattttt tacgcctatg taagaggcga gcaccgaagg tttcgttttt catattaggg
      781 aattctaatt tttcttgact taggtttctc tacgatctag tttccttagg aaaatctaag
      841 gagctcgata tgttgaaaat tgatgctata gcgttttttg gcagcaaaac aaagcttgcc
      901 aatgccgcag gagttaggct ggcaagcatt gctgcatggg gggaactggt tcctgaaggt
      961 cgcgcgatgc gcctgcaaga ggcatctggc ggggaacttc agtacgaccc caaagtttat
     1021 gacgaatatc gtaaggcaaa acgaccttgg aaggtgattc atgaaaatca ggcatgagca
     1081 catcgaatca gtgctgttgc cctggcagcc gaaaaagggc aggcgtgggt cgctaacgca
     1141 attactgaag aatatctgcg gcaggggggc ggcgaattgt ctctggtacc aggcaaggac
     1201 tggaacaatc agcagaatat ctatcaccgt tggttgaaag gtgaaacgaa agcgcaaagg
     1261 gaaaaaattc agaaactgat ccctgcggtt ctggcaattc ttccgcgtga gctgcgtcac
     1321 cgactctgta tcttcgatac cctggaacgc cgtgcattac tggcggcgca ggaagcgttg
     1381 agtacggcaa ttgatgcgca tgatgatgct gtccaggccg tttaccggaa agcacatttc
     1441 agcggtggtg ggtcgcccgg cgattctgtc gtagtgcatt gattgaaatt aatcgtgccg
     1501 gattgttttg ttcggtatca gttaaatgta acgctgcgag cgttacaagg tgaaaacaaa
     1561 tggcttcaaa ctggataaag ctcgaggtta ttacgccgga taagccggaa atattcaggc
     1621 ttgctgagat tctgaatatt gatccagatg ccgcattagg gaaggttatt cgcttctggg
     1681 catgggcgga tcaacaaatg atagacggta atgcagattg taacgctcgc ggcgttacaa
     1741 aaagtgcaat agatcgcatc acttttatgt ctggttttgc tgatgcgtta attcaggttg
     1801 gatggctggt cgaaaatgac ggtgggcttt ctctacctaa ctttgaacgt cataacggaa
     1861 aaagctctaa aaaaaaacgg gcggttacaa acgagcgagt aacaaaaata cgcgaactga
     1921 aacgaaaagg taacgctgcc agcgttacac aaacggatca aaaagcgtta ccagaggaag
     1981 aggaagagga agaggaagat ctaaatactg atctccccct cgccaaaaac gagcgtctaa
     2041 aaaattcgag ccggaggcta ttgagctgcc cgattggttg ccggaaacac tctggcatga
     2101 gtgggtccgg ttcagacagg cattgcgaaa accgattcga acggagcagg gcgctaacgg
     2161 ggcgatacgg gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg
     2221 acacagcatc gccaatgaat accagggcct gttcgcgccg aaaggtgttc ggcctgagac
     2281 gttgctccga caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag
     2341 ggggtaacgg accatgaaaa atattgcgac aggcggcgtt ctggaacgca tccgccgact
     2401 gaccccgcca catgtaaccg ccccattcag aacggtagcg gagtggcgcg agtggcaact
     2461 tgctgaaggc cagaaacgta gcgaggagat caaccgcctg aatcgccagt tgcgggtgga
     2521 aaaaattctg aatcgctcag gcatccagcc gttgcaccgt aaatgctcgt ttgcgaatta
     2581 ccaggtgcag aacgacggcc agcgatacgc gttaagccag gcgaaatcca tcgccgatga
     2641 actgatgacc gggtgtacaa attttgcgtt cagcggaaaa cttggtaccg ggaagaatca
     2701 cttagcggca gctatcggga atcgcctgct gaaagacggt cagacagtga ttgtggttac
     2761 cgtggctgat gttatgagcg ccctgcacgc cagctatgac gacgggcagt caggcgaaaa
     2821 atttttgcgg gagctgtgcg aagtggatct gctggttctt gatgaaattg gcattcagcg
     2881 cgagacgaaa aacgagcagg tggtgctgca ccagattgtt gatcgccgga cagcgtcgat
     2941 gcgcagcgtg gggatgctga caaacctgaa ctatgaggtg atgaaaacat t
//
LOCUS       DAZRVY010000264         2959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZRVY010000264 DAZRVY010000000
VERSION     DAZRVY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.267"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHXOX_004348"
     CDS             <1..314
                     /locus_tag="ACHXOX_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007642.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHXOX_004349"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHXOX_004349"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR7007643.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2201..2917
                     /locus_tag="ACHXOX_004350"
     CDS             2201..2917
                     /locus_tag="ACHXOX_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7007644.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          804 a          657 c          663 g          835 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc gccaacaata taagttttta taaaattatg gctttagaat atttttattt
     2101 ctaatagacg agatttttcc tgttatgata taatatgccg aattaacaca tgttaacgat
     2161 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
     2221 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
     2281 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
     2341 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
     2401 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
     2461 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
     2521 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
     2581 cagctatatg tttgacaatg ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
     2641 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
     2701 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
     2761 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
     2821 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
     2881 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tcttttaaag aaatctgtct
     2941 ttacggcaga tttctttaa
//
LOCUS       DAZRVY010000265         2939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZRVY010000265 DAZRVY010000000
VERSION     DAZRVY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.268"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHXOX_004351"
     CDS             37..264
                     /locus_tag="ACHXOX_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR7007645.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHXOX_004352"
     CDS             complement(265..639)
                     /locus_tag="ACHXOX_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR7007646.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXOX_004353"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXOX_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHXOX_004354"
     CDS             complement(2099..2674)
                     /locus_tag="ACHXOX_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7007647.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZRVY010000266         2930 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZRVY010000266 DAZRVY010000000
VERSION     DAZRVY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.269"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(105..1592)
                     /gene="yeeO"
                     /locus_tag="ACHXOX_004355"
     CDS             complement(105..1592)
                     /gene="yeeO"
                     /locus_tag="ACHXOX_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR7007648.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1753..1828
                     /locus_tag="ACHXOX_004356"
     tRNA            1753..1828
                     /locus_tag="ACHXOX_004356"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Asn,seq:gtt)
     gene            complement(1866..2816)
                     /gene="cbl"
                     /locus_tag="ACHXOX_004357"
     CDS             complement(1866..2816)
                     /gene="cbl"
                     /locus_tag="ACHXOX_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR7007649.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          859 a          782 c          619 g          670 t
ORIGIN      
        1 aggaatcgtg agaacgaggc gaatattagc gatgcccacc cacaatgtca aagcctgttt
       61 tttaaatttg aaatcgtttg ctgaaataat ctgcattttg tcgtttattc cgacacaact
      121 ggcttttttt cacacttttg cggttcggat cgagggtatt tccacagcca acgtccagta
      181 accattcgcc agtaaaacag cacggcccgc acagcccagt cggcaaacat tcccatccag
      241 acaccaacca caccccagcc aagcatgatg cccagcacat aaccgacgac aacccggcaa
      301 ccccacatgc tcaacataga aacccacatg gcgtaacggg catcgcgagc acctttaaat
      361 ccagcgggta gcacccatga ggcggaccaa ataggcataa ataaagcatt tagccaaatc
      421 agaatcacaa cgacatgttt aacctgtgga tcctgggtgt aaaacgatgc cataaccccg
      481 gcaaagggag ccgttagcca ggcgatggcc gttaatccaa gagtggaaag ccagaacaca
      541 tgccgcaact gaatctctgc ttgcgctatc tgccctaccc ccaaccttcg gcctgtaatg
      601 atcgtagaag cagagccgag cgcacttccg ggtaagttga taagagccgc aattgaaaac
      661 gcgataaaat ttccggcaat aacactggtc cccatcccgg caacgaacat ttgggttaat
      721 aaccgaccac tggtaaataa cactgattcg acactcgcgg gaataccaat ccccatgact
      781 tcccagataa tgctaaaatt cagcggttta aaatagctct ttaacgaaat ccttagcgca
      841 ggattaaaac caatcgccag cacccacaaa attgcaactg cgccaatata acgagaaatg
      901 gttaaaccca gccctgcccc gacaaatccc agtcccggcc aggagaaaag gccgtaaatc
      961 aatatgccgc taataataat attaagaata ttcaggctac cgttaatcaa tagcggtatt
     1021 ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc agctgctgga
     1081 taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc tgtcgtggca
     1141 tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaataagcgt tgccaacagt
     1201 acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga
     1261 tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat agcagcaaaa
     1321 aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat cgcatctttt
     1381 cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca ggcattctcc
     1441 atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt gtagctcttg
     1501 cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga gatattcaaa
     1561 gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga atcagcaaag
     1621 ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga tagaaacggc
     1681 aaacagttgg ggaatttaaa aatcgggttt gacaaaagat ttttcgccgt taagatgtgc
     1741 ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc
     1801 actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt atagcgggct
     1861 ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg ccgcttgata
     1921 tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata gttacgctga
     1981 agttgtcctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg ccgcgtatcc
     2041 agacggatta aattctcttc ctcttgttcg ccgctggatt gttcggcaac taatccgatc
     2101 ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact taatacaata
     2161 tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc cgtaatcccc
     2221 tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaatgg tgtaatttgc
     2281 gttaagggat gatcgagtgg aacaagcaaa ctatggtgcc aacgaaacca cgggaaggcg
     2341 acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc ttcgccattt
     2401 tgcaacaacg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg aacctccggg
     2461 aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg agtatgcgtc
     2521 gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca
     2581 ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc cggttcagtc
     2641 atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc atcctccagt
     2701 tcccgaatat gacggctgac gccggactgt gaggtaaaaa gcatattcgc aacctctgtc
     2761 aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa attcacggta
     2821 aactccgggc agttcagatt tcccgttatt gttaaagtct aatgcccggc ataacaaata
     2881 ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
//
LOCUS       DAZRVY010000267         2919 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZRVY010000267 DAZRVY010000000
VERSION     DAZRVY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.270"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHXOX_004358"
     CDS             <1..495
                     /locus_tag="ACHXOX_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR7007650.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="ACHXOX_004359"
     CDS             495..1085
                     /locus_tag="ACHXOX_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR7007651.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1834..2112
                     /locus_tag="ACHXOX_004360"
                     /pseudo
     CDS             1834..2112
                     /locus_tag="ACHXOX_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2172..2660
                     /locus_tag="ACHXOX_004361"
     CDS             2172..2660
                     /locus_tag="ACHXOX_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7007652.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2684..>2919
                     /locus_tag="ACHXOX_004362"
     CDS             2684..>2919
                     /locus_tag="ACHXOX_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7007653.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          833 g          692 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattacac ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacgggactg
     2881 gcgcgacgag tactgtgttg gcgacattgc tgtatacga
//
LOCUS       DAZRVY010000268         2902 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZRVY010000268 DAZRVY010000000
VERSION     DAZRVY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.271"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..978)
                     /gene="curA"
                     /locus_tag="ACHXOX_004363"
     CDS             complement(<1..978)
                     /gene="curA"
                     /locus_tag="ACHXOX_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR7007654.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLEGNDSNLL"
     gene            1159..1677
                     /gene="mddA"
                     /locus_tag="ACHXOX_004364"
     CDS             1159..1677
                     /gene="mddA"
                     /locus_tag="ACHXOX_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR7007655.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1674..2123
                     /locus_tag="ACHXOX_004365"
     CDS             1674..2123
                     /locus_tag="ACHXOX_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7007656.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2124..2357)
                     /gene="ydcY"
                     /locus_tag="ACHXOX_004366"
     CDS             complement(2124..2357)
                     /gene="ydcY"
                     /locus_tag="ACHXOX_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR7007657.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2443..2616)
                     /gene="ortT"
                     /locus_tag="ACHXOX_004367"
     CDS             complement(2443..2616)
                     /gene="ortT"
                     /locus_tag="ACHXOX_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR7007658.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2811..>2902
                     /gene="yncL"
                     /locus_tag="ACHXOX_004368"
     CDS             2811..>2902
                     /gene="yncL"
                     /locus_tag="ACHXOX_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR7007659.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          698 a          744 c          696 g          764 t
ORIGIN      
        1 caataagttg gagtcattac cctctaaacc atcagtaata tcttcgtggt agtggatttt
       61 atcctctttc acccattgcc ccatctcctt ctgaaactca tggatgcggt gaccataatc
      121 ctgagcgata ataaaccctt gcaagcgaat acgttttttc agcaccgttg ccatcaacag
      181 gggtaaacga tccggaccgg gtggtagctc tgtagcgtta tagctgctca ctaagccaca
      241 aacgggaatg cgcgcagatg tattaagtaa tggtagcact gcatcaaata ccttaccgcc
      301 cacgttttca taatagatat caataccttt tgggcacgct ttcaccagtt gttcggcaaa
      361 atcatccgcg tggtgatcaa gacaaacatc gaagcccagc acctcgatag catggcggca
      421 tttttccgcg ccaccggcaa cccccaccac cctgcaacct ttaagtttgc cgatttgccc
      481 caccgttgct cccacaggac ctgtcgccgc agctaccacc aacgtttcgc cctctttagg
      541 ctgaccgata tccagcagcc ccatataagc ggtaaagcct ggcatcccca gcacacccag
      601 cgaccacgat ggattttgcg gatgatcgcc aagtttcacc agatcatcac cactggatat
      661 gtcatagtcc tgccatccac tgtagcccag cacccagtcg ccaggctgat aatcaggatg
      721 attcgactcc acaacacggc tcaccgtgcc gccgaccatc acgccgccaa tatcaacagg
      781 cggtgaataa gatggctcat cgctcatacg tccacgcata tacgggtcca gggacaaata
      841 aactgtgcgc agtaacacct gtccttcacc cggtgtggcg acatcatctt cttcaagacg
      901 gaaattctcc ggaaccggtg cgccatgtgg acgcgaggcc agaacccaac gtcgattacg
      961 ctgcttttgt tgccccataa tgctctcctg tgcttttggc atgaatccat caatagtagc
     1021 aggctcacgg gattggtttt gtttgcagcc gtcacatccc ctctgcaaca cgcaataatt
     1081 tcatcgagga atccgttgct tcgggctgtt ttcgatagta ttgataacat catcatcatt
     1141 attttatcga ggtcagacat gtccatccgt tttgcccgca aagccgattg tgctgccatt
     1201 gcggaaattt ataaccacgc cgtgttgtat acggcggcta tctggaatga ccaaacggtt
     1261 gatgcagata accgcattgc ctggtttgaa gcgcggacca tagcaggtta tccggtgctg
     1321 gtaagtgaag aagatggcgt agtgacggga tatgcctcgt ttggcgactg gcgtagtttc
     1381 gatggttttc gccataccgt ggaacattcg gtttatgtcc atcccgatca tcagggcaaa
     1441 ggtctggggc gtaaattgtt aagccgattg attgatgaag cgcgggattg cgggaagcat
     1501 gtcatggtcg ccgggatcga atcgcaaaat caggcctcgc tgcatctgca ccattcgctg
     1561 ggatttgtcg tcaccgcaca aatgccgcag gtgggcacta aatttgggcg ctggctggat
     1621 ctgacattta tgcagttgca actcgacgaa cgcactgaac cggacgcgat tggatgaatc
     1681 agtcgctcac ccttgctttt ttgattgccg ccggaattgg tctggtagtc cagaacacgc
     1741 tgatggtgcg catcacccag acctcctcta ccattctcat cgccatgttg ctgaactcac
     1801 tggtggggat tgtgctcttt gtttctattt tatggtttaa acaaggcatg gcggggtttg
     1861 gcgaactggt gtccagcgtg cgctggtgga cacttatacc cggcttgctg ggatcgtttt
     1921 ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc ggcgacaact attgcagtac
     1981 tggtcgccag ccagctaatt ggcggtctga tgctggatat cttccgcagt catggcgtac
     2041 cactacgggc gttgttcggc ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggt
     2101 tggtagccag acgctcattt taatcagagg atggtgccgc ctttggttaa ctgttcgtgg
     2161 cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag caatagcggt gcgcaaacgc
     2221 tgctgctggg tgtagcgatc ttcccgactt aactccgcgt catcgctcag ggcgattaat
     2281 aattcgttca tatgggcaat gacgctctct tcaatcgcgg catccacgcg cgcgatgact
     2341 tcatccagat gcgacatact ttctccttaa ccacttgtga aatttatcga ttcatccaaa
     2401 tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa
     2461 cagcgccacc gggaaactca tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg
     2521 gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga aatgcgattg ctgccataac
     2581 cgcataaaaa acaagcatat gttgatagag agacatgtac gggcgtccag ccagatgaat
     2641 aaaacgcgcg caaatatgcg tcattttttg acgtgcatca agttttttca ccagtttgag
     2701 aaaaaatcat caaacagcaa cttgccctta tccatcttca gaatgagtat tattgcggat
     2761 tttcttaacc cgtactatac acataggggc aatgataaaa ggtggcaaaa atgaatgttt
     2821 ccagtagaac tgtagtactg ataaatgtct ttgctgctgt tggtttgttt actcttatct
     2881 ctatgagatt tggctggttt at
//
LOCUS       DAZRVY010000269         2900 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZRVY010000269 DAZRVY010000000
VERSION     DAZRVY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.272"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHXOX_004369"
     CDS             complement(<1..1199)
                     /locus_tag="ACHXOX_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR7007660.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHXOX_004370"
     tRNA            1492..1586
                     /locus_tag="ACHXOX_004370"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHXOX_004371"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHXOX_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZRVY010000270         2859 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZRVY010000270 DAZRVY010000000
VERSION     DAZRVY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.273"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..905
                     /locus_tag="ACHXOX_004372"
                     /pseudo
     CDS             1..905
                     /locus_tag="ACHXOX_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
     gene            908..1441
                     /locus_tag="ACHXOX_004373"
     CDS             908..1441
                     /locus_tag="ACHXOX_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7007661.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1470..1997)
                     /locus_tag="ACHXOX_004374"
     CDS             complement(1470..1997)
                     /locus_tag="ACHXOX_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7007662.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1999..>2859)
                     /locus_tag="ACHXOX_004375"
     CDS             complement(1999..>2859)
                     /locus_tag="ACHXOX_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7007663.1"
                     /translation="QRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAE
                     GQGENGNIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGF
                     RQDGDGIISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIR
                     MFLWKDNGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLST
                     WLHNHVVRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLI
                     GGIWYNVGSV"
BASE COUNT          807 a          627 c          615 g          810 t
ORIGIN      
        1 gcacaagcgg ggagaaaata ccgttactca gtacagcgaa tacctggact aatcgacaaa
       61 cattcagcgg tggcctttct ggtgaactat ccggcaatgc ttctacagct gcaaaattaa
      121 aaactgccag gaaaataagc aatgtggctt ttgatggttc ctccgatatc acattaaaag
      181 caagtcatgt tggtgcgttt gccttaggga aaacaggaag caccgttgcg aatgataaag
      241 cagttggatg gaactggagt agcggagcct ataacgcaac tattagtggt gcatcaacgt
      301 taattattca tttttatatg ggagaaggaa gttgtcctgc agctcagttt cggattaatt
      361 ataaaaatgg cggtattttt tatcgttcag cccgtgatgg ttatggtttt gaagccgact
      421 ggtccgaatt ttacaccacc agaaaacctt cagcaggaga tgttggtgca ctgccgttat
      481 ctggtggtca actgaatggt gcactgggta tcggaacatc cagtgctctt ggcggtaatt
      541 cgattgtatt gggtgataat gacacgggct ttaaacaaaa aggcgatggt aatctggatg
      601 tttatgctaa taacgtccat gttatgcgct ttgtctccgg aagcattcaa agtaataaaa
      661 ccataaatat tacgggacgt gttaatccct cggattacgg taactttgat tcccgctatg
      721 tgagagatgt cagacttggc acacgagttg tttagaccat gaagaaaggc gtgatgtatg
      781 aaaaatcagg ccatgtaatc acggggcttg gtattatcgg tgaagtcgat ggtgatgatc
      841 cggcagtatt cagaccaata caaaagttaa ttaacggaac atggtataac gtatcgcagg
      901 tataatcatg cagcatttaa aaaacattgt cgcaggtaat ccaaaaaccg ttgaacaata
      961 ccagctaaca aagaattttg atgttatctg gttatggtcc gaagatggaa aaaattggca
     1021 tgaggaatta aaaaactttc aggaggatac aataaaactg gcttacacag ttgagggaat
     1081 aattattgct atagacaagg atgtatcagc aattaatcca gaaggtttaa gtgtcgttga
     1141 gttgcctgat attacagcaa atcgccgggc tgatatttcg ggcaactgga tgttcaaaga
     1201 tggtgcggtg ataaagcgaa cttataccga ggaagagcaa aggcaacaag cggaaaatga
     1261 aaagcagagc ctgctgcagc tcgtcaggga taaaacccag ctgtgggact ctcagctaca
     1321 gctgggcatc atttccgatg agaataaaca aaaattaacc gagtggatgc tctatgcgca
     1381 gaaggttgaa tccacagaca cctccagcct gccagtaacg tttcccgaac aaccagaatg
     1441 agagaaggcc cgctatcggg ccttaatttt tattcaggct tttgtggcca ttcaggattt
     1501 gccgtatcca cacggctgac cagaacactg tagcgttccc atgcttccag tcgtgtgcgt
     1561 tcctcgtctg ttgccatatt cagcctgaca gcgcgttcca gcggctggat gactgattca
     1621 gcttcggaaa gcaatgcggc cttttgtgat tcggcctgtt gttgcagttc ctgttctgta
     1681 tattccctct ttactacttt accatccaga aacatccatt ttccgttatt gtctgcgtga
     1741 cgatttgccg taatatcagg aacttcaact atgcttagcc cttcaggatt aatagttgaa
     1801 acgtctttac tgacagcaac gatgatgttg ttctcatcgt aagcaatttt tatggtgtct
     1861 ggctgaaaat tcttttgttc ctcataccag tttttgccat cttcgcaaaa gagccatacc
     1921 acgcccgctt ttttagttag ctggtattgt tccggtgttt tcggattccc ggccttaata
     1981 tttttcaggt gcatcatctt aaacactccc cacgttgtac catattcccc caatcaattt
     2041 ctgaattggc cttctggcaa cagtgtcgac aatatcatcc gcattgctgt taattgcggc
     2101 tgttaaaaca taacctgacg catcgccaaa gccgtagtcg cgccatacgt ttttatattc
     2161 aatgctgcca agacgaatat cccgaacgac atgattatgc agccatgtgc ttaaccagtt
     2221 gttttcccac actgaaccat aaatatcgcc actagacgca acttgcgcac cacttcccag
     2281 cgcaaatccc ccatctgttt taaaaatgaa atcaccacca ccatctgaac cgttattaat
     2341 atgaacacca tcaccaccgt tatctttcca caaaaacata cgaattcttc cgtcttcgtt
     2401 ggcgaatgta atgtggtggc ggtaattact ttttaatcta aaattgctgc cattagattc
     2461 aatatcttta taaagatgta atcctaactc atcaatatgt ccgatgcgcg aaccatttga
     2521 atagaagcta ataataccat ctccatcttg cctaaatccg gtgtcgttat ctcccagaac
     2581 aatggagttt tcacccagtg cgttatcagt agctccaata gctagacctc ctttaatttt
     2641 ggccccgtgg ctgacagata tagcacctgt tctcagattt atagcgaatg gccttaatgg
     2701 gccgatatta ccgttttcgc cttgtccttc agccgtcgga atgaaatgaa gaaaatcttc
     2761 tgaacgacgg aaaattagac cgaaggtatc attgaaaatt cgcagcgcat tcaccgtgcc
     2821 aattttcagt tctccggtca ttttatcgcc ggaacgctg
//
LOCUS       DAZRVY010000271         2776 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZRVY010000271 DAZRVY010000000
VERSION     DAZRVY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.274"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..678
                     /locus_tag="ACHXOX_004376"
     CDS             <1..678
                     /locus_tag="ACHXOX_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7007664.1"
                     /translation="NQPHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHL
                     LYALNIAVRTAPDSSVKALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWR
                     EEAYTLDELADYLDLSASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWL
                     DAVIQRVEMYNASLPVPLSPPECRAIGKSIAKYTHRNFTPETFAQYVADTHTLTYVFV
                     PLALTPR"
     gene            1011..1886
                     /locus_tag="ACHXOX_004377"
     CDS             1011..1886
                     /locus_tag="ACHXOX_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR7007665.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1894..2670
                     /locus_tag="ACHXOX_004378"
     CDS             1894..2670
                     /locus_tag="ACHXOX_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR7007666.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          761 a          664 c          660 g          691 t
ORIGIN      
        1 aaccagcctc atgcgcagtt ctggctggtt tttgacgttg atcgcgaggg ggcagcgatt
       61 gactggagcg accggaacgc tcccgcgccg aacatcaccg taaaaaatcc tgtgaacggt
      121 catgctcacc tgctctacgc gctcaatatc gccgtgagaa ccgcgccgga cagctcggtt
      181 aaggcgctga aatacgccgc tgcgattgaa cgttcgctgt gtgagaaact ttgtgctgat
      241 gtgaactaca gcggactgat ttgtaaaaat ccgttccacc tggaatggca ggtgatggag
      301 tggcgagagg aagcctatac cctcgatgaa ctggctgatt atctcgattt gagcgcctca
      361 gcgcgccgta gcatcgataa acattacggg atggggcgaa actgccacct gttcgaaatg
      421 acgcgcaaat gggcttacag ggcgattcgt cagggctggc ccgaattctc acagtggctt
      481 gatgccgtga ttcagcgtgt cgaaatgtac aacgcatcgc ttcccgttcc gctttcaccg
      541 cctgaatgtc gggctattgg caagagcatt gcgaaataca cgcacaggaa cttcacgccg
      601 gaaactttcg cacagtatgt ggctgatacg cacacattaa cgtacgtttt cgttccattg
      661 gccctcaccc cccgataacg acccaggtag cgcgccgtat tccccccgcc ggccgttttc
      721 ttcgacatgt acatcaggcg cggaactcat cggggcgcgg gaatgaaatc ccattcagac
      781 tccggataga ttcaagcaag cccacttatc gtcaaaatcg gtgttgcaaa aacgggagtg
      841 accatagatc cgttttccaa gcggacccca taatatcggc tggtaaacat cactcgctag
      901 tgcattcttt ttgttggtaa tgataatcat ttacattcat tctttgatgt tttatcataa
      961 ttaatgtttt gtcaataaat atatggaaac atattgtttc agatgggaat atgagaaagt
     1021 tcatccttat ttctatgata acgttagcta gtgttagtct gggagcctgc agacagaatg
     1081 tcaccataaa gcaggatgcg ccagggcaga aagctttcct gactgacggg cagatttacg
     1141 atttgcacag tggcaaaatt atttctagca gcgagctttt ggcggatctt gctaccgctc
     1201 gacatcttat tattggtgaa aaacacgata atgccgagca tcatcagatt gagctttggc
     1261 ttattcaaaa tttgctcatc cagcgaccac aggggagtgt gttgcttgag atgttgacgt
     1321 cagagcaaca gccccgagtt aaccaagtta aatgttggct gaaagataat cctgtggttc
     1381 gagatagccg tgttcaggaa ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg
     1441 gcgacatcgt catgcagcta ttacgtgggc cttacccgct gctcaatgcc aacattggcc
     1501 gggagcagat gttggcactg tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg
     1561 cacctgtggt acaagaagct ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg
     1621 aaagccagca actgacgtca atgctctcta ttcagcagca gcgggatcgc tacatggccc
     1681 gccagcttct gagtgccccg gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa
     1741 aaagcatggg cgtaccgtta cacatggaag atcttgccac cggcacccat ccagtggtgt
     1801 tgatgcttgc agaaaaagga atgaacatca cagtagatca tgctgactac gtgtggtttg
     1861 tagcgccaga tacaacaaaa aggtaaacca tggatgatga atattctcca cttctcacag
     1921 tcggtaaaat ggagcagctg gtttatctgt tctctactgc ttcatggact gattttcctt
     1981 gcgtttatct ggcgtttttc cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg
     2041 ctgcaatggg cagaaattat agaggctcct tcttctccac tttcgcttcc ggtgggaatc
     2101 gctcaacagg agtccgcagt gacagaagaa aagcaacaaa ctgaggacag acaacaacgt
     2161 cctgtcactg aagacagtga tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat
     2221 ggagaaaaga aaaagacacg tccacctcgt aagataaagg ctcaaaccag cgacagcaac
     2281 cccacagccg tttccagtaa tgctgcacct caggctctgg tcgaatcgtc ccgtattgct
     2341 gcgcctttta acagtgactc aacaaagcga gataattctg aggcatcttg ggaaagcaga
     2401 gtaaaaggcc acctaaaccg ttataaacgt taccctggcg atgccagaaa acgtgcacga
     2461 accggcacag ccgtcgtgac ttttaccgta aacacggaag gcactattgt cagtagtttc
     2521 ttggaaatct cttcaggaac cctttctctt gaccgcgaag ctattgctgt tttagaacgc
     2581 gctcaaccac ttcccaaacc accgcctgaa atattggagg gaggattatt taaagtaaaa
     2641 atgccgatta cctttaagtt gaaggagtaa agggataatt ggccgttcaa gttcatttaa
     2701 ctgaaattta attttcattt ttatgctgaa cattttatca aacttttcca ctgtcgaaaa
     2761 aatcaaaaag acagta
//
LOCUS       DAZRVY010000272         2728 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZRVY010000272 DAZRVY010000000
VERSION     DAZRVY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.275"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHXOX_004379"
     CDS             complement(423..680)
                     /locus_tag="ACHXOX_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007667.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHXOX_004380"
     CDS             complement(677..1174)
                     /locus_tag="ACHXOX_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR7007668.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHXOX_004381"
     CDS             complement(1376..1834)
                     /locus_tag="ACHXOX_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7007669.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHXOX_004382"
     CDS             complement(1831..2328)
                     /locus_tag="ACHXOX_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR7007670.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHXOX_004383"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHXOX_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR7007671.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacca
//
LOCUS       DAZRVY010000273         2703 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZRVY010000273 DAZRVY010000000
VERSION     DAZRVY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.276"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHXOX_004384"
     CDS             <1..247
                     /locus_tag="ACHXOX_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR7007672.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(287..1495)
                     /locus_tag="ACHXOX_004385"
     CDS             complement(287..1495)
                     /locus_tag="ACHXOX_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7007673.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHXOX_004386"
                     /pseudo
     CDS             1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHXOX_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2022..2690)
                     /locus_tag="ACHXOX_004387"
                     /pseudo
     CDS             complement(2022..2690)
                     /locus_tag="ACHXOX_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          747 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgcg acgcggcggt
      121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg
      181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgctg acagaaaccg
      241 atattgacat cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct
      301 gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg
      361 caagaacggc gtgccccgcc gctaaaatgt tacgagcacc atttacatcg gcgttcgctg
      421 tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttctct ttcgctgtat
      481 gaccacagta cgcgcaacgc tggcttgtat acgctggcgg aacagcaagc acctgaccgc
      541 cacgccagag ctgcttgtac tcaagctggc ggtgcatttc ataccagccc tgatccagta
      601 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
      661 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
      721 tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat
      781 gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc
      841 ttaactgtcg ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct
      901 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
      961 ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc
     1021 acgtaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat
     1081 tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct
     1141 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
     1201 cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
     1261 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
     1321 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
     1381 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
     1441 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc
     1501 aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt
     1561 tcctgatgca tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg
     1621 acagaaaaac tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt
     1681 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg
     1741 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
     1801 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
     1861 agtagctgcg gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
     1921 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
     1981 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
     2041 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
     2101 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
     2161 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
     2221 tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
     2281 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     2341 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     2401 gtatagttca aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc
     2461 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     2521 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     2581 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     2641 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     2701 ctg
//
LOCUS       DAZRVY010000274         2651 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZRVY010000274 DAZRVY010000000
VERSION     DAZRVY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.277"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHXOX_004388"
     CDS             complement(25..315)
                     /locus_tag="ACHXOX_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR7007674.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHXOX_004389"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHXOX_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR7007675.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHXOX_004390"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHXOX_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR7007676.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHXOX_004391"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHXOX_004391"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2651)
                     /locus_tag="ACHXOX_004392"
     CDS             complement(2171..>2651)
                     /locus_tag="ACHXOX_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR7007677.1"
                     /translation="AVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAR
                     EARRQEIEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGD
                     ADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          698 a          718 c          600 g          635 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat ttcctgacgt ctggcctctc
     2521 tggccgcttc cagcatttct ggtgtcacag tgaatggggc tatctcaccc catttgccac
     2581 tttgcagttc ctgccagatt tgctgacccg tcggtgcgac atcatcagcg gtggctgtgt
     2641 aggggactgc c
//
LOCUS       DAZRVY010000275         2587 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZRVY010000275 DAZRVY010000000
VERSION     DAZRVY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.278"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHXOX_004393"
     CDS             196..1149
                     /locus_tag="ACHXOX_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7007678.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHXOX_004394"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHXOX_004394"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR7007679.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHXOX_004395"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHXOX_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          636 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZRVY010000276         2582 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZRVY010000276 DAZRVY010000000
VERSION     DAZRVY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.279"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..402
                     /locus_tag="ACHXOX_004396"
     CDS             <1..402
                     /locus_tag="ACHXOX_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR7007680.1"
                     /translation="IIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            399..878
                     /locus_tag="ACHXOX_004397"
     CDS             399..878
                     /locus_tag="ACHXOX_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR7007681.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHXOX_004398"
     CDS             complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHXOX_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR7007682.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHXOX_004399"
     CDS             complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHXOX_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR7007683.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2252..>2582
                     /locus_tag="ACHXOX_004400"
     CDS             2252..>2582
                     /locus_tag="ACHXOX_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007684.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          755 a          590 c          561 g          676 t
ORIGIN      
        1 ataatcaaag aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct
       61 cttatcctgc aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac
      121 ttcgaggaca gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata
      181 tctcttttaa gttttcaaaa atacgctata ggatggagtg caacaattca ttttgtagca
      241 caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga
      301 ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc
      361 accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa
      421 ttatattatg tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac
      481 agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc
      541 catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc
      601 aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct
      661 tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa
      721 ttaagagcaa tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt
      781 atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata
      841 aaatagagcg agttttcaat ccgcatgact ttaaataaga caatttttag aaataagccg
      901 gataaaaaaa tagctttatc cggcactcat attacagcca gttcttgcgc ttaaagtaca
      961 gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc
     1021 acttcagttc tggcataaac tcaaagttca tcccatagct ggaagcaacg agcgtcggcg
     1081 gcaggaatac cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga
     1141 taaaacccat cgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg
     1201 gcagcaggga ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta
     1261 aacgcgcttt acgcaccagg aagttgagcg cgcgctgggt atccatcaga cacaggcgaa
     1321 ctttccagcc gatatcttcc agttccgcca gagtggagag cgcctcgtcg tactcatcgc
     1381 cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag atattttcaa
     1441 tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcagc tcgtaggcgt
     1501 taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt
     1561 cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac
     1621 cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt
     1681 cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct
     1741 ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc
     1801 atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt
     1861 ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg
     1921 gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg
     1981 gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag
     2041 ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc
     2101 ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca
     2161 gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca
     2221 ggataaaaaa atccaacatc caggacctat aatggcgcat cggctactca ttggtaaagg
     2281 aatgatcacc ttgaatctca aacggatttt tctcgccctc accttacttc ccttgtttgc
     2341 tgttgccgct gatgattgcg cactctcaga tccgatgctg accgtacagg cgtataccgt
     2401 taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gccaatggcg aagcgtgggg
     2461 aactttacat gctctgctgg tggatatgaa tagtcagggt caggtgcaga tggcgatgaa
     2521 cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca
     2581 ga
//
LOCUS       DAZRVY010000277         2561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZRVY010000277 DAZRVY010000000
VERSION     DAZRVY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.280"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..803)
                     /locus_tag="ACHXOX_004401"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="ACHXOX_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(856..1452)
                     /locus_tag="ACHXOX_004402"
     CDS             complement(856..1452)
                     /locus_tag="ACHXOX_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR7007685.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHXOX_004403"
     CDS             complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHXOX_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR7007686.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2096..>2561)
                     /locus_tag="ACHXOX_004404"
     CDS             complement(2096..>2561)
                     /locus_tag="ACHXOX_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7007687.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          784 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatgagctgg ataatttgat ctgttgagtt actgttggat caaagtttgt
      181 atctgtatag aattcaattg taagggtctg ataaatacca cctacagccc agtatttacg
      241 ggtgctattc gcattattgc aactccaata taacacgtct tcgcttggat tataccacga
      301 aaaatattgt tctgcagagt caccaatgta tatcccaggt gaactaacgt tagttacggt
      361 accgtaagct gaccacatgt ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt
      421 attaaataat ttatgggagc catacatttt acctgagtca accattgcat tttcaatacg
      481 cattgcgcta ccacttgtgc tatcacttct gttgcaataa agaaaaccac caccagaagc
      541 ggtatctaac tgactccact gtaattttac acctgtaggt ccaagctgcg tcggtttgta
      601 gaaaactaaa ttatttgtac ttgttgcata tctgaatatg acagtagcat cgccattcgg
      661 ccccgtttct ctccattttt gatctgacgg accaacgccg taactattcc caacaatata
      721 tcctacgtta tcatcagtgc cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa
      781 aaatgtctga agaatttctt cattatcact ccaccatcta ctgctgtaac agtcgcagtt
      841 atttgttttg atctattatt cgtagtacac atcaactaat atatgactca ttacttggcc
      901 agatatgaca tccagcgcgg gatcgatttt ttgcattctg gcataaaatt tccatgaaga
      961 attatgcagg gcagaagtca ttactggata cacggaacga gaatttcctg ctgaactaat
     1021 aacattgaat atattggtcg ggtcctgaat ggaaaagaga acgacaccga catttttagc
     1081 cccggttgcg acttcattgg caaatatttg ccttgaacct gcagccatga caccgctaag
     1141 ggctctaaaa tcaatatgta actgattaag cacatcgcca gttccctgta atgcacagtc
     1201 tgatactgtg acagtgaact cttttccacc tggttgatag ctttcaggag taacattatc
     1261 tgaaaaatat cccaccccaa cgacgccaag atcaatatta ccctggttac tgattccgct
     1321 tttgcaagtg ctgtttttta cattggcaac aaggtcaata tcctgcccag cgatggctgg
     1381 ataggtaatg ggaaactgta gtgacagaaa cagaattatt ctcttcatca gcttacgttg
     1441 agtgggatgc atatatagtt cctggatctg tcagtctgtt agttataggt tactgtaaat
     1501 gtggcaggtg cagaaaattt acccgcagtg acattgctta cggttttatt tttttcgacg
     1561 accagtcgag cactcatcgg ataataaaca accgcgttgg ctgcattgga gatagtgacc
     1621 tcttgagatg ctggtgtcac acaactaaat tgtgttgcgc tccctgttgc cggagtttcg
     1681 gtgctccaga cttcaacggc gacggcactt gctttatctg cggctgtgga tccatttgcg
     1741 agccccgcac cgtcattagc agtaccttcg catgtggctc gcttggttaa ttttatttgt
     1801 gcttttttat tggggatacc cgcacagtct ttgaatttga gtttgaatgt tttaactttg
     1861 gtcttggcat tgatttcaga aatatataca tcgccaaagt caacaacaga aatgtcctga
     1921 tcagagtcat ttactaatgt agcggtacaa gtacctattt ctacggtagt aatcacggtc
     1981 agcccaatgc tgtctgttgc cgcataagag ggtacgctga acaatgaggc agccaaaacg
     2041 acactgctgg ataatccata acgtaaattt ttaaatgtca tcttaattat ccttcttatt
     2101 gatagatgaa attaaacgtt gccagtgcac gaaaattccc cggtacgcct tgccctgcat
     2161 ctgtttcacg tagcgcaacg gtcatttcaa gacccttatc gggctgcatc tcgtctgtgc
     2221 tccagcgtat cttttccgca ctgttaggtt taaggaaagt ggcatcatca gtagtccgtt
     2281 ttttgaaacc cataccgata ttacttgtcg tcgaagatga atcaccagac tgcggtataa
     2341 taagcttagg tgagcttgat gatgcatttc cggtcagagt ggtatcaatc caactgatgc
     2401 cactgctgca cccactagca accagtttaa aatcagcctg agattctgtc gttttattcg
     2461 cgatcttatc cagacccatc ttagggattc tcaatgtgta gttattattg ccatcattcg
     2521 tgacgttatt accagtaagt gtgatgttac aggttgtcgc t
//
LOCUS       DAZRVY010000278         2556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZRVY010000278 DAZRVY010000000
VERSION     DAZRVY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.281"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXOX_004405"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXOX_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR7007688.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHXOX_004406"
     CDS             complement(390..1178)
                     /locus_tag="ACHXOX_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR7007689.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXOX_004407"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXOX_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR7007690.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXOX_004408"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXOX_004408"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR7007691.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHXOX_004409"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHXOX_004409"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR7007692.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZRVY010000279         2545 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZRVY010000279 DAZRVY010000000
VERSION     DAZRVY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.282"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..81
                     /locus_tag="ACHXOX_004410"
                     /pseudo
     CDS             1..81
                     /locus_tag="ACHXOX_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            828..1673
                     /locus_tag="ACHXOX_004411"
     CDS             828..1673
                     /locus_tag="ACHXOX_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR7007693.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1684..1872
                     /gene="dicB"
                     /locus_tag="ACHXOX_004412"
                     /gene_synonym="ftsT"
     CDS             1684..1872
                     /gene="dicB"
                     /locus_tag="ACHXOX_004412"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR7007694.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1860..2060
                     /locus_tag="ACHXOX_004413"
     CDS             1860..2060
                     /locus_tag="ACHXOX_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR7007695.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2145..2420
                     /locus_tag="ACHXOX_004414"
     CDS             2145..2420
                     /locus_tag="ACHXOX_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007696.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          743 a          556 c          599 g          647 t
ORIGIN      
        1 gatgcaaaac gcctccagca actggagcca aatgcaggca ctggggcacg catctggctg
       61 gctaaagaag cgctggaatc tggcgattat gatagcgaag aagccttcta caaagcagaa
      121 ggccgtacag gatattcacc gggtcttggc ggggtataaa taccatgcgc attgactgaa
      181 ttcgcaaaca aaaacagacg cgcgatacct ggatagtcgg tctgcatagt cagatatctg
      241 gctgcgaatt tgataaatat caccgccctt ttcggaaaca taagttccgt ccgggagttg
      301 attatacgaa ccatctccat gggggatcgt tctcctgaat cctagtgaga gcatatattt
      361 atgaagccct tcgtaatcct ctggctcagc attatatagt tctactctgg cgagatacgt
      421 tggcatattc atttccttac tggttgtgtg agaacttcag taaagatacc accaaagccc
      481 ggaggtggtg aaataaaacc gggcacaaca cgaaggcgca tttccggtat ccataaagag
      541 tcggtcttgt ctgttaaatt taaatggtgg gagtgcgcct ccggttgtaa ataacgacat
      601 tgctgtgtga agtaccagtt ggcggcatcg gtttaattgc tggctgatgt ccgccctttt
      661 taaagtgaat tttgtgatgc ggtgaatgcg gctcagcgca cgcggaacag ttaaaacagt
      721 aaggaaaatc ccttattccg gcgttaattg ttaactggtt aacgtcacct ggaggcacca
      781 ggcaccgcat caacaaagtt cacttcggtg atgaaaggaa agagaaaatg ttgaatgtag
      841 ctattgaaaa ccagaacggg tggaattata gtgcacctgc acctcataaa acgggtgccg
      901 ggattgctac cccgaagtca ttcacggcgc ataaccgcgc tcaggcggtt tttttatgcg
      961 taatgcacag ccacattcaa attatggtgg ggcgtgcggg gcagtcgcaa gactggccgg
     1021 gttccgtgat gaccggttgt agcaaccctg tacgtctcac cacccatgag attgctacct
     1081 ccggtggtga gttaatcaaa ttcatcgcgg aggctgccat catggctact accctttctc
     1141 acccttgtgt aaccgtagaa aacggtcgcg ctgtcactac gtctgttgcg gttgcagagt
     1201 tcttccacaa acgacacgac aatgtgttac gtgccatcgc aaatattgaa tgctcagata
     1261 aatttactgc cctcaatttt gaagcgagcg aatacaccga ctcaatcggg cgcaaactcc
     1321 caatgtacca aatcaccaaa aacggcttcg ttttcctggt tatgggcttc actggcaaaa
     1381 aagccgctgc attcaaagaa gcctacattg ctgagttcga ccgcatggag gccgaactgc
     1441 gccagaataa caccccacct gctgacaaga tgattccggg tgatggtcgc actctggttg
     1501 ttcacttcga caaattcggc aatgtcgaat tcaccgaaac cgttcctgat ggcgcactgg
     1561 tctgtaccct ggagactttc cggttttatc tggaaaaaca gggatggact cttgttaacc
     1621 ggggcgcaat taaaaatatg actgtggaac aattactcaa aattcattgt tgaggacgca
     1681 ataatggaga cgttattacc aaacgttaat acgtctgaag gttgttttga aattggtgtc
     1741 actatcagta accctgtatt tactgaagat gccattaaca agagaaaaca cgaacgggag
     1801 ctattaaata aaatatgcat tgtttcaatg ctggctcgtt tacgtctgat gccaaaagga
     1861 tgtgcacaat gaatacagta tttgcactcg ttctgacggt ttttcttgtt tccggtgaac
     1921 cggttgacat ggttactggc gtatacagct caatgaaaga atgtatgact gccgcagcga
     1981 aacagaaaat tcccggtgac tgttatccgg tcgataaagt tattcaccat gataataacg
     2041 aaatcccggc aggtctttaa aacagttccg taataaatat ccggtttcat tcttatatgc
     2101 cagcaatggc aggggttcgc tcaaccttaa ttaaggagaa aaacatgatt accagttatg
     2161 aagccactgt tgtaactact gatgacattg ttcacgaggt taatctggaa ggaaagcgta
     2221 ttggctacgt gattaagaca gaaaataaag aaaccccatt cactgtggtt gatatcgacg
     2281 gtccatcagg caacgttaaa acacttaacg atggtgtaaa aaaaatgtgt ctggtgcaca
     2341 taggaaagaa tctgcccgca gaaaaaaaag ccgaatttct ggcaactctg attgcaatga
     2401 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacac tgtgcaggcc tgagtgaaga
     2461 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
     2521 tgagcaccga taaacaagtt taccc
//
LOCUS       DAZRVY010000280         2540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZRVY010000280 DAZRVY010000000
VERSION     DAZRVY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.283"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(14..88)
                     /locus_tag="ACHXOX_004415"
     tRNA            complement(14..88)
                     /locus_tag="ACHXOX_004415"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            complement(112..196)
                     /locus_tag="ACHXOX_004416"
     tRNA            complement(112..196)
                     /locus_tag="ACHXOX_004416"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Leu,seq:tag)
     gene            complement(205..281)
                     /locus_tag="ACHXOX_004417"
     tRNA            complement(205..281)
                     /locus_tag="ACHXOX_004417"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Met,seq:cat)
     gene            complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHXOX_004418"
     CDS             complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHXOX_004418"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR7007697.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2279..>2540)
                     /locus_tag="ACHXOX_004419"
     CDS             complement(2279..>2540)
                     /locus_tag="ACHXOX_004419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007698.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT          610 a          639 c          629 g          662 t
ORIGIN      
        1 attgtttctt cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg tatcaaaaac
       61 cggtgcctta ccgcttggcg ataccccatc cgtacaacgc tttctggtga atggtgcggg
      121 aggcgagact tgaactcgca caccttgcgg cgccagaacc taaatctggt gcgtctacca
      181 atttcgccac tcccgcaaaa aagatggtgg ctacgacggg attcgaacct gtgaccccat
      241 cattatgagt gatgtgctct aaccaactga gctacgtagc catctttttt tcgcgatacc
      301 ttatcggcgt tgcggggcgc attatgcgta tagagccttg cagcgtcaac ctctttttca
      361 aggaaaattg ctcgaaagtg actgtttggt taggttgcga acagcgtggc gctatattcg
      421 tcaattattg tttactttgt gttttttccc caccctacag ccattctttt gtcatacagg
      481 atgaaattcg gaattcaaca atggtggtgg gaaattaatc tatgaaatac tggcctacag
      541 tgatgagttg tcaaacagtg atgtggcaaa cccggaacat ttccttactg catatcagaa
      601 tcaacaagca cctcaataac tgaaacagcc ccggatttca ccggggctgt ttcgcatttc
      661 ttacttatac gccgactggt gaacgccaac cgcacgacca gacggatcgt ccattttctt
      721 gaacgcctca tcccactcaa tcgctttagc ggaagaacac gcgacggacg ggccgcccgg
      781 cacgcactca gcggcgctcg gaagcgggaa cagttcttca aagatctccc ggtacagata
      841 cgcttctttt gaggttggcg tgttgtacgg gaagcggaag cgggcagttt ccagttgctg
      901 atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg atccaactgt aaccgacgcc
      961 atcggagaac tgctctttct gccgccaggc cacgcttgcg ggcaggtatg actcaaaaca
     1021 ttcacgcagg atgtgttttt ccattttgcc gttaccgcac attttatcct gcgggttaat
     1081 gcgcatcgcc acatcaagga attttttgtc gaggaacgga acgcgtgctt ccacgcccca
     1141 ggctgacatc gctttgttgg cgcgcgcgca gtcatacata tgcagggcca gcagtttacg
     1201 caccgtctct tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa gataaccgcc
     1261 aaacacttca tcagaacctt caccggacag caccatttta atgcccatcg ccttgatctt
     1321 acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat aagtttcgat
     1381 gtggtaaatc acgtcgcgga tggcatccag accttcctgt acagtgaagt gaatttcgtg
     1441 atgcaccgtg cccagatggt ttgccacttc ctgggctgct ttaagatccg gtgaacccgg
     1501 cagacctaca gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt cctgatcttc
     1561 cacgcgacgg gctgcgtatt tcttggtgat agcggaaata attgaggaat ccagaccacc
     1621 agaaagcagc acaccgtaag gcacatcaga catcagatgg cttttaacgg aatcttccag
     1681 agcctgacgc agctcgtttt tgtcggtcac gttatctttc actgcatcaa agtcgaacca
     1741 gtcgcgatga taataagaac ggatttcgcc gtcctggctc cacaaatagc tccccgccgg
     1801 gaactcttta atcgtgcggc aaactggcac cagcgatttc atttctgagg ccacatacag
     1861 ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc cccagatggt cgcgaccaat
     1921 caggtaggca tctttttcgc tgtcgtacag ggcaaaggca aacatgccct gcaagtcgtc
     1981 gagaaattcc ggcccttttt cctgatacag cgcgaggatc acttcacagt cagaccctgt
     2041 ctggaactga taacgatcgc catattcagc gcgcaatgcc tggtggttgt agatttcacc
     2101 gtttaccgcc agtacgtggg ttttttgttg gttgtagaga ggttgcgccc ccgcgttaac
     2161 gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta tcgctggcat aaataccgga
     2221 ccagtccggg ccacgatgac gcatcaggcg tgacagctcg agtgctttct tacgctgctc
     2281 aactgcgtct gttttgatat cgaatacgcc aaaaattgaa cacataacct tctccgttaa
     2341 cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa gcagcactgt
     2401 gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg attggtgaaa
     2461 aaggggctgg tgtgaggatg tatttattga tgaatcgata atttttagcg ggttttattg
     2521 aatggttata ttttacgggg
//
LOCUS       DAZRVY010000281         2530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZRVY010000281 DAZRVY010000000
VERSION     DAZRVY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.284"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHXOX_004420"
     CDS             <1..656
                     /locus_tag="ACHXOX_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7007699.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHXOX_004421"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHXOX_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR7007700.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHXOX_004422"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHXOX_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR7007701.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHXOX_004423"
     CDS             2258..2494
                     /locus_tag="ACHXOX_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR7007702.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          655 a          559 c          645 g          671 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata
//
LOCUS       DAZRVY010000282         2524 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZRVY010000282 DAZRVY010000000
VERSION     DAZRVY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.285"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1310
                     /locus_tag="ACHXOX_004424"
     CDS             <1..1310
                     /locus_tag="ACHXOX_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007703.1"
                     /translation="YTQSNAFNFTLEMNRLKVRKPTIGIIAQGFNDGTYSEEVKITVE
                     NKQCGLVEDVSFTGGFGNLFASWTKSTERDYAGAIISIVNGTNTRIFTSYAPEFDSIP
                     NITDGEYKVKMGFFDVFGTDDIQYCPEQTISINSKYQFTEDDAEAINGILDLEDRLTG
                     TINDAVKIANKNTSTVISASEARTNDKITASEKTLSTQITGVNSSLSQKLSTVESTAN
                     GNKASIQTLSQTVTDNNKAQTTAVSQLRSDVNGQIATVNQSMSTKADKSTVDAQYSLS
                     VQANGTVAGMRLVASSGTSNNSAIYFAANKFIVSGTDTATVGGTAPFAIVNGTTYLKT
                     AMIQAASIGTGYIADAAITNLKLASGSVNTLNVVDGCITTAKISNTIQSTNYVANKTG
                     WQINKAGTILINGTGGTGRMVISNNIIQIYDNNNTLRVRMGLW"
     gene            1468..1764
                     /locus_tag="ACHXOX_004425"
     CDS             1468..1764
                     /locus_tag="ACHXOX_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007704.1"
                     /translation="MAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQTYTVSVP
                     NMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKWTA"
     gene            1766..2422
                     /locus_tag="ACHXOX_004426"
     CDS             1766..2422
                     /locus_tag="ACHXOX_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007705.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
BASE COUNT          793 a          480 c          554 g          697 t
ORIGIN      
        1 tctatacgca aagtaatgcc tttaatttca ctcttgaaat gaaccgtctg aaagtacgta
       61 agcctaccat tggtattatc gcacagggat ttaatgatgg tacatattca gaggaagtga
      121 aaataaccgt agagaataaa cagtgtggtt tagtagagga tgttagcttt acgggtggtt
      181 ttggtaattt gtttgcttca tggactaaat ctactgaacg tgattatgca ggtgcgatta
      241 tcagtattgt taatggtact aatacacgta tattcaccag ttatgcacct gaatttgatt
      301 caataccaaa catcactgat ggtgagtata aggttaagat gggattcttt gatgtgtttg
      361 gtactgatga tattcaatat tgtccagaac aaacaatcag tatcaactcc aagtaccagt
      421 ttaccgaaga tgatgcagag gcaattaacg ggattcttga tttagaagat cgcctgactg
      481 gtaccattaa tgatgcggtc aaaattgcca ataagaatac cagtactgtt atcagtgcat
      541 cagaggcacg taccaatgat aaaatcactg ccagtgagaa aacgttaagt actcagatca
      601 ctggggtaaa tagttcctta tcacagaaac ttagcaccgt tgaaagtact gccaatggta
      661 acaaggccag tatccagaca ctatcccaga cggtaacgga taacaacaaa gctcagacta
      721 cagcagtctc acagctacgt agtgatgtga atggacagat tgccactgtt aaccagtcaa
      781 tgagtactaa agccgataaa tctactgtag atgctcagta ctcactatca gtacaggcta
      841 atggcacggt agctggtatg cgtcttgttg cgagtagtgg aacgagtaac aactcagcaa
      901 tctatttcgc tgcgaacaag ttcatagtct ccggtactga cactgctact gtaggtggta
      961 ctgcaccatt tgccatagtc aacggtacta catacttgaa gacggcaatg atacaggcgg
     1021 caagtatcgg tactggttac atcgctgatg cggcaattac caatctaaaa ctggcctccg
     1081 gctcagtgaa tacattaaac gtggttgatg gttgtataac tacagccaaa atctcaaata
     1141 ctattcaaag tactaattac gtagcaaata aaacaggctg gcaaatcaat aaagcaggta
     1201 cgatcctaat caatggaacg ggtggtactg gtagaatggt aatcagtaat aatattattc
     1261 aaatctatga caataataat acgttacgtg tacgtatggg tttatggtaa taaataattc
     1321 agggattatt aactgtaacc tcatttcaaa aggatacata aatgaaatct aatgaacgta
     1381 ctttcaatag taccagtact aacccattca gtattagcgg aaaagaaaca attggaatga
     1441 agaaaaggat ttacaggagt aagagctatg gcacagggat tacaatgttg gaatgcagcg
     1501 ggtgtattag tagtagatct gactgattat aacatacgtt ttatgggtac ttatactgtc
     1561 aaggctactt ctgcacagac ctatactgta tcagtaccaa atatgaaaac tacaggctgg
     1621 tttgtacact atgcccccgc cagtgatacc tttaatgagt ggtcagcctt ttgtaataat
     1681 gggtcattta ctgcggttta tttgccaaca tatgcaccgt tagctggtaa ctacactttt
     1741 aacgtatata agtggacggc gtaagatggc aggtttcgag gtatataatt cagcgggtgc
     1801 actgactata gatagtacta acaagtcgat aatgacgggt gcagtaaagg ccatgggaaa
     1861 tctaactgat acgggatact acacaggctt tacctgtgcc ttcggtaatg gtggatcgct
     1921 cggtttcgtt atgccatcag tagtagctaa caggaacaca acacagtact ggttccagat
     1981 acagaaagac ggggcatggt gtttccctgg tgcctatatg ttccagcctg gcatgggcag
     2041 gttcatgaca agtagtcaca cggctacccc cacatctggt tttcttgatg tgttcagtga
     2101 agaaggtact ttaatctggt ctgctgcatc agcggcaaca atgccacgta tcaggggatt
     2161 tttgacagca cctgcggcta ccgatctcag tactgccatt acggtaacgt ctccagtagc
     2221 tgatccctgg ttctgctggt cacaatgtcc ggggaacata tcagatgatg gtacagtaat
     2281 tgggtactct ggtctggtta tcaggcgtaa ttccagtact tcattcagtc tacagtacgt
     2341 ttcaaagaac cagaaaacct accgccaggc aatggggaat aatggtatcc agatagcatt
     2401 ggcgacattt acagggtatt aatacaaaaa caataacaat aacaataaca ataacaataa
     2461 caataacaac aaagggattt cagtatggat atcggtgcta ttttagctct tgttatttct
     2521 ggat
//
LOCUS       DAZRVY010000283         2507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZRVY010000283 DAZRVY010000000
VERSION     DAZRVY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.286"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(158..2425)
                     /gene="adiA"
                     /locus_tag="ACHXOX_004427"
     CDS             complement(158..2425)
                     /gene="adiA"
                     /locus_tag="ACHXOX_004427"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR7007706.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          580 a          616 c          686 g          625 t
ORIGIN      
        1 ataaataaca ggcaaaaaaa agcgcggggt ggggccgcgc aaaaagtatg acatttttgt
       61 ttgcagcaat ttttatgaaa acgttgccat actcaaaagg cctaaggcca ggcggcgtaa
      121 tgttatttaa acaattacgc cttcagcgga atagtggtta cgctttcaca cacataacgt
      181 ggtaaatacc gtcaataatt tcagtccctt cagtttcgtg ttcaaatcca gggaaatggt
      241 ggtcccagga ttgcagcgag cgtaaataac ttacttgcgg actgttttta tcgccgaagt
      301 tttcaccaga cagcagcatc gggattcctg gcggatatgg gataactgag tttgccgcga
      361 tgcgtcctgg cagattttca atggatacca gttcgacatt gttgtcgaca atcgcgttgt
      421 acgcttcacg cggggtgatt tccgccaccg acaggccgga ataggcttcg ttcaaccgtg
      481 cgccagggtt gttttctttc agccaggcaa acatggtgtc acccagatcg tgaatcccca
      541 tgttcgcgta agtgtcagga tattgttcaa caagttccgg catcacctgc gccagcggtg
      601 tgttggcgtc atagtggcgt ttgaaggagc aaagggtgtt aaccagagtt ccccatttcc
      661 cacgggttac gcccatagag aacaggaaca taatttggaa gtcagtggtg cgggtaggca
      721 caatgccgtg gcgaccaagc caggcagtga ccagcgccgc cggaacaccg gtttcttcca
      781 gttcaccatc ttcacccatt cccggagcaa ggatgctgac tttaatcggg tcgagcatac
      841 tccagttatc cggaatatct ttgaagccgt gccagctttc gcccggatgc attacccagc
      901 agtcctgaac ggtggtcagc agtttggttg gtgcgtcagc aaagtcatag gttttgccgg
      961 tttgtgggtc ggtgacgact tctttgttcc acggtttgaa gaaccagcta ccgtcagcgg
     1021 tgaactcttt atacagccgc gccatcgcct gacggaaatc aaccgcttcg tcgatcactt
     1081 cctgcgtcag tgacaggccg ctgttgccgt ccatcatcga caccgccacg tcgttggatg
     1141 cgcagatggc atacagcggg gaggtggtgg catgcatcat gtaggcctgg ttgaagcggg
     1201 agaagttaat cgccccacga ccttcacgta catgaatata agaagcctgt gaaagcgcat
     1261 tcagcagttt gtgggtggag tgggtggcga aaacggtagg accgttgtga tcgccaggtt
     1321 cgccgcgcat ggcatagtga tcggcataga tcgggttgaa acgtgcatag ccgtaccagg
     1381 cttcgtcaaa gtgcagacga tcggaggttt tttccagcag atcctgcgct tctttagcgt
     1441 tataacacac gccgtcatag gtgcagttgg tcaccacgca gtaagacggt ttttgcccgg
     1501 ctttgtcttt agtcagcggg ctttcactga ttttcttctg caaggtttca ggttgcattt
     1561 cctgcggata gattggcccg ataatgccgt agcggttgcg gcttggcacc atgtagaccg
     1621 gtttcgcgcc tgtcagcatc aagccttgtt cgatggattt atggcagtta cggtcaacga
     1681 ccacgacatc gttatcggtc atgcaagcct gcatgatggt gcggttagag ccggaagtac
     1741 cgacgactac cgaccaggag cgatcggcac caaatacgcg tgcggcatat ttttcgcttt
     1801 cgccaaatgc gccagtatgg tcaagcaaag aaccgaggga agttcgttcg atgcccatat
     1861 cggtgcggaa cagattttca ccatagtagt catggtagaa acgtccggcg ggtgttttgg
     1921 taaaaccaac gccgccctgg tggcctggcg ctgcccagga atattcatgg atgtcgctat
     1981 atttcatcag cgcgctgaac agtggcggca acagctgctg gcggtagcgg gtcatcgcgg
     2041 caacagcgcg tccggcgata aagtcggcgg tatcttccag aatccaggcg aattcatcga
     2101 caagctccag caggtcgcga tccattgcgg cgagggcttt ttcccgatcg cccaacagga
     2161 agaccggcac gttttgttgg cgctcatgaa gcttaccgat caattgtctg acgttttgat
     2221 gttcgtccgg atgttccatt tgatagctga acatcaggca gtcaatggct tcgtttgaag
     2281 agagaatggc aaaaccatca tcaaaggagg tggatttaat cacggtaaca ttttgctggc
     2341 ttaaagcatc tgccagacgc tcaacggcgt taccgaccca ggtgtcttga tggagaaact
     2401 cgctttcaac aattaatact ttcatcattg cttacccggt tatgaaggaa tcttcgttgc
     2461 gggcaagtat cttccggcct tttcgggctg taaagcgcgt gaaaaac
//
LOCUS       DAZRVY010000284         2507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZRVY010000284 DAZRVY010000000
VERSION     DAZRVY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.287"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHXOX_004428"
     CDS             <1..275
                     /locus_tag="ACHXOX_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007707.1"
                     /translation="ITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLR
                     LQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(681..815)
                     /locus_tag="ACHXOX_004429"
                     /pseudo
     CDS             complement(681..815)
                     /locus_tag="ACHXOX_004429"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(828..1649)
                     /locus_tag="ACHXOX_004430"
     CDS             complement(828..1649)
                     /locus_tag="ACHXOX_004430"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7007708.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1638..2507
                     /locus_tag="ACHXOX_004431"
                     /pseudo
     CDS             1638..2507
                     /locus_tag="ACHXOX_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1918..2034
                     /locus_tag="ACHXOX_004431"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          658 a          563 c          592 g          694 t
ORIGIN      
        1 ccataacaga ggccaccctg tacgaaaacg ggctgtatac gcgccacatc agactcagtc
       61 gcccagtatc tgacagcaaa taccccttgc cacccggatc tcagctcatt aaaaatatga
      121 ccttacgact gcgcctgcag gatcagcagg aagaaacatc cgtgacatta tttcagggaa
      181 aaatgagtac cgacggaaat aactattaca tcatcaaagg taaagtaagc tcagtacttc
      241 tgttattaaa aatgctgcag attaatcacg cctgatctct gtcccggtat tttagcccga
      301 ggtatattct gaaatatcat tcatcatgac cggcgtttat acaaacgccg gtcattcgct
      361 aacagcttca ccgttccttc ccccatccag gttccgcatg agcgtgttgt aatgttcata
      421 agcgtattat caggattgtc tggaaaattc ccgatacttt ggactattac tcctgaacaa
      481 ttcgaaattc agcagattaa aggtaagcgt taacggaggt aagcgttaac ggaggtaagc
      541 gttaacggag gtaagcgtta acggaggtaa gcgttaacgg aggtaagcgt aaaccgactg
      601 ccgtatgtag ccattaagcc agtattggta acgtaggtgc ccaccttttc aaatgatctg
      661 cgccccaaat cttggacatc ctatgaaaaa tgtttaagtc gatattgaac cggactcatg
      721 ccatcgagtt tctctttaat gcgatggtga ttgtagtatt caatgtaatc aattatatct
      781 tgctttagat gatgtaactt attgtattta tttaggtagt aacattctta ttttaaagta
      841 ctaaaaaagc tttccattgc tgcattatct aagcagttac cgcgtcggga catgctttgt
      901 gttatgccat tatcactcaa ccaacgttgc caacgaggca tctgatattg ccacccctga
      961 tcactatgca acaagggctt atcgtcttta ttgagttgtt ctaccgcatc taatagcatg
     1021 ctctttacca ttgatgctaa tgggcgctca gtaaggttat atgagactat ttctccatta
     1081 tacaggtcca tgattggtga taaatagagc ttcctgccat cgaccttaaa ctctgtgaca
     1141 tctgttaccc acttttgatt tgggcggtca gcataaaatt gtctcttcag taaattatta
     1201 caagttcgcc cctgttctcc acgataggca cggtacttct tgcgcctgat aagtgagctg
     1261 atacccagtt ccttcattaa ctttctgatg gttttaccat taagtaaaat gccctcgtta
     1321 cggcaggcaa gtctgatgcg tcgataacca taacgccctt tatgctgatg gtatattctc
     1381 aatattactt gtttaacaag ttgatatgga tctggcgcat taaggcgttt cacgtgatag
     1441 taaaatgtac tacgtggcaa atccgcgata accagtaatt gtgataatgg atgacattgc
     1501 cttaactcat taataatgat tactttttct tttgttccaa tcgtaatttt tcctgagcta
     1561 aggctttcag cttttttaga taatgaaccg ccccgggaat cctggagact aaactccctg
     1621 agaaagaggt aaacaggatg actaaaaata ctcgttttcc cccgaagtcc gtcaacgggc
     1681 agtccgtatg gttctggaaa gtcagggcga atatgactca caatgggcgg taatttgttc
     1741 cattactcca aagattggct gtacaccgga gactctgcgt gtctgggttc gccagcatga
     1801 gcgggatacc aggggcggtg atggagggct caccaccgct gaacgtcagc gtctgaaaga
     1861 gctggagcgt gagaatcgtg aactgcgctg cagtaacgat atccttcgcc aggcttccgc
     1921 ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa taatgccact gctggataag
     1981 ctgcgtgggc agtacggggt cggaccggta tgcagtgaac tgcatattgc cccgtcaacg
     2041 tattaccact gttagcaaca ggacatcatc ctgataaacg cagtgcccgt gcgcagcacg
     2101 atgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg
     2161 tgcgtaaagt ctggcgccag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg
     2221 cgcgcctcat ggcggttatg ggacttgtcg gtgttctccg gggtaaaaag gtccgcacta
     2281 ccgtcagccg gaaaaccgtt gccgcaggtg accgcgtaaa ccgtcagttc gtggcagaac
     2341 gtcctgacca gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct
     2401 atgtggcgtt catcattgat gtgtttgccg gatacatcgt gtggtgacga gtctcatcgt
     2461 ctatggaaac gacattcgtg ctggatgcac tggagcaggc gttgtgg
//
LOCUS       DAZRVY010000285         2435 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZRVY010000285 DAZRVY010000000
VERSION     DAZRVY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.288"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHXOX_004432"
     CDS             complement(<1..458)
                     /locus_tag="ACHXOX_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR7007709.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHXOX_004433"
     CDS             complement(497..784)
                     /locus_tag="ACHXOX_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007710.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHXOX_004434"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHXOX_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR7007711.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHXOX_004435"
     CDS             complement(1417..2082)
                     /locus_tag="ACHXOX_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR7007712.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..>2435)
                     /locus_tag="ACHXOX_004436"
     CDS             complement(2251..>2435)
                     /locus_tag="ACHXOX_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007713.1"
                     /translation="AVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          607 a          638 c          542 g          648 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcac
//
LOCUS       DAZRVY010000286         2329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZRVY010000286 DAZRVY010000000
VERSION     DAZRVY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.289"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHXOX_004437"
     CDS             20..238
                     /locus_tag="ACHXOX_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR7007714.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHXOX_004438"
     CDS             240..605
                     /locus_tag="ACHXOX_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7007715.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHXOX_004439"
     CDS             602..1267
                     /locus_tag="ACHXOX_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007716.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHXOX_004440"
     CDS             1267..1632
                     /locus_tag="ACHXOX_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR7007717.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHXOX_004441"
     CDS             2087..2242
                     /locus_tag="ACHXOX_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7007718.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          495 c          628 g          579 t
ORIGIN      
        1 ctcgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAZRVY010000287         2272 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZRVY010000287 DAZRVY010000000
VERSION     DAZRVY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.290"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(134..1138)
                     /gene="argF"
                     /locus_tag="ACHXOX_004442"
     CDS             complement(134..1138)
                     /gene="argF"
                     /locus_tag="ACHXOX_004442"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR7007719.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1300..1716
                     /gene="rraB"
                     /locus_tag="ACHXOX_004443"
     CDS             1300..1716
                     /gene="rraB"
                     /locus_tag="ACHXOX_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR7007720.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1762..2265)
                     /locus_tag="ACHXOX_004444"
     CDS             complement(1762..2265)
                     /locus_tag="ACHXOX_004444"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7007721.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          581 a          559 c          552 g          580 t
ORIGIN      
        1 gaaaattaaa tcctccggaa gatttacgta tagcaataaa aaaattgggg tggaataatt
       61 gaactatcac tgcataaaat aatgtgacgc cgggatgttt ttgtatttcc cggcatcttt
      121 atagcgatat caattattta ctgagcgtcg cgaccatcac cgctttgatg gtatgcatgc
      181 ggttttccgc ctgatcaaat acaatgctgg cggcagattc gaagacctca tcagtcactt
      241 ccattccgcc atgtaggcca aactcttccg ccattttttt gccaagcgtc gtttggtcgt
      301 catgaaacgc gggcaggcag tggaggaatt tgacctccgg gttaccggtc aactgcatca
      361 tcttgctgtt cacttgataa tcacgcagca atgcaatgcg ttcagcccat ttctcttttg
      421 cttcacccat cgacacccat acatcggtat agataaagtc agcaccttca actcctttag
      481 ctacatcttc cgtcagcgta atattcccgc cattttgttg tgccagggcg cggcattccg
      541 taaccagcgc agcttccggc cagcatgcct gtggcgcgac cagacgcaaa tccagaccgg
      601 taagcgctgc agcttcgagc atcgaattgc ccatgttgtt acgtgcgtca cctgcataga
      661 ctagcgtcat ttcgttgaac gttttgccgg gcaaatgctc ctgcatggtg aggagatccg
      721 ccagcagctg cgtggggtga aactcattgg tcaggccgtt ccataccggc acgccagcgt
      781 attccgccag tgtttcgaca atctcctgac catagccgcg atactgaata ccgtcataca
      841 tgcgaccaag cacgcgggca gtgtctttaa tcgactcttt atgaccaatt tggctgccgc
      901 ttgggccgag ataagtaaca cgagccccct ggtcatatgc ggcaacttcg aaagagcatc
      961 gggtacgggt cgagtctttt tcgaagatga gcgcgatgtt tttaccagtg agtttggctt
     1021 cttctttacc gcttttctta tcggctttca gcttcgcggc taactgcagc aggctgttga
     1081 gttcagctgg cgtgaaatcg agtaatttca ggaaatgctt atgataaaac ccggacatag
     1141 gtccctcctg tggctaacgc cttaatgaat taaaattcaa tttatatgga tgattattca
     1201 tttgcaagtc taaagcataa atctttatca caaaggtgga ggcaatgtca gtggtgtgtg
     1261 acaataagag tatcggcagg acattaagag gaatgagcca tggcaaaccc ggaacaactg
     1321 gaagaacagc gtgaagaaac acgtttgatt attgaagaat tactggaaga tggcagcgat
     1381 ccggacgcgt tgtacaccat cgaacaccat ctttccgcag acgatctgga aactctggaa
     1441 aaagcggcag ttgaagcgtt taaactcggt tacgaagtga ccgatccaga agagctggaa
     1501 gttgaagatg gtgatatcgt gatttgctgc gacatcctca gcgagtgcgc gttgaatgcc
     1561 gatctgatcg atgcccaggt tgaacaactg atgacgctgg cggagaaatt tgacgttgag
     1621 tacgacggtt ggggcactta ctttgaagat cctaacggcg aagatggcga cgatgaagat
     1681 tttgtcgatg aagacgatga cggggttcgc cactaattaa ttgattaaaa tgcccgatag
     1741 cacatactag cgggcatttt tttagagttc gcgtagcatc cgcacttcac aatcgacatg
     1801 gcccgtgcag ccaagcgcat agtcgatatg ctcaaagccc aaatgctcat aaagcgcaat
     1861 ggcttccttt aaaaaagcgg tcgtttccag atagcagcgt ttgaagccca tctctcgtgc
     1921 ctgctccatc gccattaagg ccagtttttt cgctagccct ttaccgcgaa tagccggaag
     1981 aaaatacatc ttttgcagtt cgcaaatgtc cgactcactc ccggttaatg gcgcaatccc
     2041 accgccgccg accacttcac cctcgtactc aaccacccaa tatgcatggc caggctgact
     2101 atacacttga tacagctcgt caagattcgg atcggcgacg gtgtagcctt tatcagcggt
     2161 aagaccgtat tcggcggata cctgacgaat gacgcgggcg atagcaggat tatccagcag
     2221 cgtcaggcga cgcattacag gtgattgcgg cgcaatgtta ttcatagttt aa
//
LOCUS       DAZRVY010000288         2266 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZRVY010000288 DAZRVY010000000
VERSION     DAZRVY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.291"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHXOX_004445"
     CDS             <1..302
                     /locus_tag="ACHXOX_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007722.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..2254
                     /locus_tag="ACHXOX_004446"
                     /pseudo
     CDS             391..2254
                     /locus_tag="ACHXOX_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
BASE COUNT          717 a          404 c          430 g          715 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta ataaaactga ccactaatat
     1381 tggtctggcc gatgatgctg agtggctatt ctataatgct agttttggca tgcagcatcc
     1441 catacttcac gcgataatgt actgtaagac tgataattgt tatgaagcaa atgaaaggaa
     1501 acatcctgac agtaatcagt ttaataccag atataaatac tcccctactc atgaggaggc
     1561 tgctgtctgc tgcgtaccga atacagcaag aaccataccg gcttttgtcg aaaatatgtt
     1621 tcaagaaaat aacaatggtt ttactgcatt attttatggc ccgtgtgagt tttatggcac
     1681 atttaataat gttgcaatta aaattacaca attaactgaa taccctcatg atttaagcgt
     1741 aaaatgtctc attgagcctg agtcatctgt tcgttttgct ctcagtttcc gttaccccgg
     1801 ctgggcaaaa atgatgatta ttaatggtgt tacttttact aaaaatgata ctcaaaactc
     1861 acttattatt ttagatagaa catggcaaca tcatgatgtg attgacatta aatttatagc
     1921 tgatatacag tttaatactg acttatgtgg tgacacttat atttccagag gtcccgtttt
     1981 atatgcaatt gagcttgaga gcgatatttt aatcaaaaaa aacctgctga acgggaaata
     2041 ttttgactca ggatacatac cctgtagtag agctgatgaa tctatccagt tctcttatgc
     2101 tgatatggaa tctttttcat attcaacctc accagagggc aaacaataca ttgaaggctg
     2161 tttttattta aacaagacta aaatagtcag aaaacttata ccatttggtc aaaccattct
     2221 tcgcaaagta actttctcca atatagagag ttaatatttt cgagtg
//
LOCUS       DAZRVY010000289         2216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZRVY010000289 DAZRVY010000000
VERSION     DAZRVY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.292"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            186..695
                     /locus_tag="ACHXOX_004447"
     CDS             186..695
                     /locus_tag="ACHXOX_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR7007723.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            692..2110
                     /gene="phrB"
                     /locus_tag="ACHXOX_004448"
     CDS             692..2110
                     /gene="phrB"
                     /locus_tag="ACHXOX_004448"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR7007724.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          552 a          539 c          583 g          542 t
ORIGIN      
        1 actgttattt tctaaaaata aaaaatacaa aaaatgaatg cagctcggga aatagtagaa
       61 taattctcac tcagatgaag ttgataatat ttttaccaac aaaatacttc cccgcaaaaa
      121 ctctgcccca taaaattcat ttaccatcgt ctacacttac tcctttatac cgttcaggag
      181 cagtaatgaa tctacaacga tttgatgaca gcaccctaat ccgtatcttt gcccttcatg
      241 agttacatcg actgaaagaa catggcttaa cgcgcggggc gcttctcgat tatcacagcc
      301 gctataaact cgtctttctg gcgcattctc agccggagta ccgcaaactt ggcccgttcg
      361 tggctgatat tcaccagtgg caaaatctgg atgactatta caaccagtac cgccaacgcg
      421 tagttgtttt gctttctcac cccgccaacc cgcgcgatca caccaatgtt ttgatgcacg
      481 ttcagggtta ttttcgcccg catattgatt ccacagaacg ccagcagctg gctgcgctta
      541 tcgacagtta tcgccgtggc gagcaaccac ttcttgcgcc gctgatgcgt atcaaacact
      601 atatggcgct ttatcatgac gcctggcttt cagggcagcg ttatttcgaa ctttggccgc
      661 gtgtgattaa cttgcgccat tcaggagttt tatgactacc catctggtct ggtttagcca
      721 agatttacgt ctgcacgata atctcgcact ggctgccgcc tgccgcaatt cgtctgcacg
      781 cgtgctggcg ttgtatatcg ctacaccacg ccagtgggcg acgcataaca tgtcgccgcg
      841 tcaggctgaa cttatcaatg ctcaactgaa tgggctacaa atagcgcttg cggaaaaagg
      901 tattccttta ttgttccgtg aagtggatga ctttgtcgcc agtgtcgaaa tagttaaaca
      961 ggtgtgcgcg gaaaacagcg ttacccacct gttttataac tatcagtatg aagtgaatga
     1021 gcgggcgcgg gatgtggaag ttgaaagagc gctgcgtaac gtggtgtgtg aaggatttga
     1081 tgacagcgtg atcctgccgc ctggcgcggt gatgaccggt aatcacgaga tgtacaaagt
     1141 ctttacgcct tttaagaatg cctggctgaa acggctgcgg gaagggatgc cggagtgcgt
     1201 cgctgcgcca aaagttcgta gtagcggatc gatagagccc tcgccatcca ttacgctgaa
     1261 ttatcctcgt cagtctttcg atactgcgca ttttccggtg gaagaaaaag cggcgattgc
     1321 gcaattacgc cagttttgcc agaacggtgc cggagaatat gagcaacaac gagattttcc
     1381 ggcagtggaa ggcaccagcc gtttgtcggc cagcctggca acgggcgggt tatcgcctcg
     1441 ccagtgcttg catcgcttgt tggctgaaca gccgcaggcg ctggacggtg gggtcggtag
     1501 tgtctggctt aatgagctga tctggcgcga gttttaccgt cacctgataa cgtatcaccc
     1561 ctcgttgtgt aaacatcgtc caattattgc ctggacggat cgtgtacagt ggcagagcaa
     1621 tcccgcacat ttacaggcct ggcaggaagg caaaacggga tacccgattg ttgatgccgc
     1681 tatgcgtcag cttaacagca ctggctggat gcataacagg ctacggatga ttacagccag
     1741 ttttctggtg aaagatttat tgatcgactg gcgcgaaggc gagcgatatt tcatgtcgca
     1801 gctgattgat ggtgatttgg cagccaataa cggtggctgg cagtgggccg cttcaaccgg
     1861 aaccgatgca gcgccgtatt ttcgtatttt caacccgaca acccagggcg agaaatttga
     1921 tctcgaaggt gagtttatcc gccagtggct accggaacta cgcaatgtac cggggaaatc
     1981 ggtgcatgag ccgtggaagt gggcgcagaa agcaggtgtg aagctggatt atccgcaacc
     2041 gatagtcgag cacaaagagg cgagagtaca aacgttggca gcgtatgagg cggcgcggaa
     2101 ggggaaataa cagacgcgtc aggcaatcga gcccagatgc cggatgcggc gtgaacgcct
     2161 tatccagcct acaaatttgt gcaaattcaa taaattgcag gaaatacgta ggcctg
//
LOCUS       DAZRVY010000290         2093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZRVY010000290 DAZRVY010000000
VERSION     DAZRVY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.293"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHXOX_004449"
     CDS             complement(<1..123)
                     /locus_tag="ACHXOX_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR7007725.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHXOX_004450"
     CDS             384..719
                     /locus_tag="ACHXOX_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR7007726.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHXOX_004451"
     CDS             complement(1000..1131)
                     /locus_tag="ACHXOX_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR7007727.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHXOX_004452"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHXOX_004452"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHXOX_004453"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHXOX_004453"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="ACHXOX_004454"
     CDS             complement(1986..>2093)
                     /locus_tag="ACHXOX_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7007728.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZRVY010000291         2089 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZRVY010000291 DAZRVY010000000
VERSION     DAZRVY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.294"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHXOX_004455"
     CDS             complement(60..1025)
                     /locus_tag="ACHXOX_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR7007729.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHXOX_004456"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHXOX_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007730.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZRVY010000292         2088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZRVY010000292 DAZRVY010000000
VERSION     DAZRVY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.295"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHXOX_004457"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHXOX_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR7007731.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHXOX_004458"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHXOX_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR7007732.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZRVY010000293         2005 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZRVY010000293 DAZRVY010000000
VERSION     DAZRVY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.296"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHXOX_004459"
     CDS             <1..389
                     /locus_tag="ACHXOX_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007733.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHXOX_004460"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHXOX_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR7007734.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHXOX_004461"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHXOX_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR7007735.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHXOX_004462"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHXOX_004462"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHXOX_004463"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHXOX_004463"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          483 c          487 g          506 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtt
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZRVY010000294         1991 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZRVY010000294 DAZRVY010000000
VERSION     DAZRVY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.297"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHXOX_004464"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHXOX_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR7007736.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHXOX_004465"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHXOX_004465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR7007737.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHXOX_004466"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="ACHXOX_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR7007738.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          584 a          455 c          386 g          566 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc a
//
LOCUS       DAZRVY010000295         1944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZRVY010000295 DAZRVY010000000
VERSION     DAZRVY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.298"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..487)
                     /locus_tag="ACHXOX_004467"
     CDS             complement(<1..487)
                     /locus_tag="ACHXOX_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007739.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FF"
     gene            complement(620..931)
                     /locus_tag="ACHXOX_004468"
     CDS             complement(620..931)
                     /locus_tag="ACHXOX_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007740.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            1124..1675
                     /locus_tag="ACHXOX_004469"
     CDS             1124..1675
                     /locus_tag="ACHXOX_004469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007741.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            1681..>1944
                     /locus_tag="ACHXOX_004470"
     CDS             1681..>1944
                     /locus_tag="ACHXOX_004470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007742.1"
                     /translation="MKKLLIGLLIALSTGCTTTHSFMDKAYVGYVDDKAVVLFDAGTR
                     NGVKYYRPQFQSDCGRIYNKIYHDDNTSTGCLIRDSRIAELHPY"
BASE COUNT          587 a          386 c          392 g          579 t
ORIGIN      
        1 tgaagaaata attattgaat atagcttcat cttttggcga tactttccaa aaggtaagtg
       61 attgcaattc gctttttaat gaggctaact taatgtttaa tagatcgata acttctcgtt
      121 cagttttaac taaatgtgcg aaattttttg gttctatatc tggttcagta ttgttaaggt
      181 tgcatatatt ttcatatgtt ttcaacatct ccaacgaaaa aagatcgcaa ttagtccaga
      241 acttagtaac ttgatttaaa gcattttcct ttattttagt ttgaaaccaa tttttagcgt
      301 tccatgctgc atatagggct gctccagcca taatgagatt ggcaatagaa ctaacccagt
      361 cactaaccga tccccactcg aaaccttcag aaccaacaaa gagtgatttt attagtacta
      421 aaattacaac aaccagtaat actgctacgg tcgataaaag aacgatattt aattttctac
      481 aatccatcga aagtagttcc tgaacgcatt aaaggggcaa atactttacc atccgaaaca
      541 caatcaagta cactacccgt tcacgtactg ctcacatgtt gggtttgtta ttttgttggg
      601 gtgagcagta cgtgtatcct cagaagggta gttccccctg atcacagaac caatcacctt
      661 cttcactgct ttgattcatc cggtactcag cagatagata tgctaaccca tcaataacac
      721 ttattagtgg tgtttcacag gtaagtacaa aacaatctct ctctatagta cctaaccagt
      781 attccctgta ctgattctgg aagaataccc tatccccata atgaaattct gttagtgcag
      841 atccccaata acacacggtt gcccagcgac cgttatgtga gaacttctga acccctcgcg
      901 taatggatgg atcatagccg tctttgctca tttgttaccc tcctctatgt attactgtat
      961 atatgtacag tattgcagtc agtttcccaa aaatccagca cccctttgtt taccactttg
     1021 aatgattaat tctattgtaa tgatcaataa aattgatcga tttaattgcc aatgttttaa
     1081 aaatgatcaa ttatcaataa cttaaagcgt aacgagcaaa gttatgaaca ttgataattg
     1141 ggcaataccg ataacaagtt tgggtggtca gttgctgggg gttgagttgg tcagccatgt
     1201 agaacgcgag ggaatcaggc agatcggcct atgggaagaa gaagtacttc atgggcaact
     1261 aatgcttatt gaaagcaagg cggcatggtt cagggataac ggtttgtatt gtgtgattac
     1321 tactggccgt ggtgaaccat acgagtactt accattcctt cggcagatga caagggatat
     1381 gaaagtacat aaccagcagt ggcttgatga tctcggtgta catggtggtt ctgctattcc
     1441 tctggttaca ggtgatttcg aggttgcccg attaaataga aggtacacag aagagaacat
     1501 tgaccgtctg atctttccag tactcataaa aagcattcgc cagtactgcg agaaggtgat
     1561 cgtacccgtc agagataaaa cgtattaccc aattctcaga gaggccggtg tatgggcggt
     1621 acagggagag ttcaggccaa tgcgttttga gcaatgtgaa aaattaatag gataacaata
     1681 atgaaaaaat tactaattgg gttactgatt gcccttagta ctggatgtac tactacgcac
     1741 agcttcatgg ataaagctta cgttggctat gtagatgaca aggccgtagt actgtttgat
     1801 gcaggaacac gtaatggggt gaagtactac cgtcctcagt tccagagtga ttgcggtcgc
     1861 atctataaca agatctatca tgatgacaat acaagtactg gatgcctcat acgagattcc
     1921 cgcattgctg aactacatcc ctat
//
LOCUS       DAZRVY010000296         1746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZRVY010000296 DAZRVY010000000
VERSION     DAZRVY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.299"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            38..1138
                     /gene="ompC"
                     /locus_tag="ACHXOX_004471"
     CDS             38..1138
                     /gene="ompC"
                     /locus_tag="ACHXOX_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR7007743.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1302..>1746
                     /locus_tag="ACHXOX_004472"
     CDS             1302..>1746
                     /locus_tag="ACHXOX_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007744.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLENIYIDHNNLESLDLKNQHS"
BASE COUNT          536 a          352 c          357 g          501 t
ORIGIN      
        1 aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt
       61 ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga
      121 tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa
      181 gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa
      241 tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc
      301 caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt
      361 tggctctctg gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga
      421 tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg
      481 taccaccggt ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa
      541 ctttgccgcg caatatcaag gcaaaaatga acgtactgac aacagtcatc tttatggtgc
      601 tgactacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg
      661 ctttggtatc ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc
      721 tgctaatcct ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta
      781 tgatgccaac aacatctact ttgcagctaa ttacgctgaa acattaaaca tgaccaccta
      841 tggggatggt tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt
      901 cgacttcggc ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg
      961 ctacggcgac caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa
     1021 aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg
     1081 tgccgtagat attcggaccg ataacatcgt cgctacgggc attacctatc agttctaacg
     1141 tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc
     1201 cttaagtaaa aatgagcaat ttttgatatt tctggtcatt cacctcaatt aatattattc
     1261 catatcctcc ggggacatct tactataata tttattgatg agtgattatg accaatatta
     1321 atacagcttg tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag
     1381 aaaccatctc cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata
     1441 atggtgaaga aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg
     1501 catctcttga tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta
     1561 atacgttaaa tctggaaaac aattgtctaa cttgtcttga cttagctgac aatgccagtc
     1621 tcgttaatat caacctcagc gttaataaaa ttaacacgat aacctttcca aaagaatcaa
     1681 aactggaaaa catttatatt gatcataaca atctggaaag tttagattta aaaaatcagc
     1741 attcat
//
LOCUS       DAZRVY010000297         1735 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZRVY010000297 DAZRVY010000000
VERSION     DAZRVY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.300"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1138
                     /locus_tag="ACHXOX_004473"
     CDS             <1..1138
                     /locus_tag="ACHXOX_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7007745.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="ACHXOX_004474"
     CDS             1150..1599
                     /locus_tag="ACHXOX_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR7007746.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          524 a          288 c          498 g          425 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttgctcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaa
//
LOCUS       DAZRVY010000298         1727 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZRVY010000298 DAZRVY010000000
VERSION     DAZRVY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.301"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            471..818
                     /locus_tag="ACHXOX_004475"
     CDS             471..818
                     /locus_tag="ACHXOX_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR7007747.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(838..1428)
                     /locus_tag="ACHXOX_004476"
     CDS             complement(838..1428)
                     /locus_tag="ACHXOX_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007748.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1425..1685)
                     /locus_tag="ACHXOX_004477"
     CDS             complement(1425..1685)
                     /locus_tag="ACHXOX_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007749.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          397 a          478 c          423 g          429 t
ORIGIN      
        1 ggctgtctat cttacagaca gcctttttat aataacagaa agaatatata tcatacttaa
       61 cggaagagat aatatgaaaa cagtacccgt ataaacagca agcaatccca tttttccagg
      121 tgtatcagca aaaaaaccgc tgttctagaa atcaggccgg gtaaatttca gagctgtatc
      181 ttcaatatac cattttgcaa ccggatacaa aaccattccg cagagagaaa tacaccagaa
      241 cagtaatctg tatttaaaat cataatccca tgacatatac agcatatatc ctcccgtcac
      301 ccatccccac cacatattat taaaataata ttttctgttc atctgccagt tttcctttct
      361 tttgcacttt tgtcagtgta ctgatgcatg acaacataac cacagcatga taataataat
      421 caataacaat aagctgtgtc acgtttacat catggaacgg aggtgctcct gtgacttttg
      481 agcagaaaaa agcccgggcc attgccctga tggacagcaa aaagatgtgg cggagcaatt
      541 atgcccctcc tcttctgcgt attctctggc ggctgggtat ccggcttccg ccattaccct
      601 ttatgccgtt ctggcaggtc acggtgctga cggggggctt atggggcatt tcctggggat
      661 gtgcaatgtg gtttatctac tgggggccgt caggaatggt ggcaggtgag gccattatta
      721 taagtatcac ggggggtttt ttgtccggac tgctcatggc ctctttccac tggtggcgca
      781 ggaaagttaa ccgactgcca ccatgggatg atgtgtaaac cgtgcacagg cgtgttctta
      841 cggagcggca ctttcattta tctgtacgcg aacctccatt cctgcatcgt gacaggcctg
      901 cagccactgc gccacttcca gcggatcgcc ctcccggcgt accacccagc cttctttatt
      961 ccataactgc agacaggtgc tgccgtcgag acgcaccaca aaatccccac ggcaggcctg
     1021 atacgttatg ccgttcagct ccaccgccag atcagtggca gaagtgccgc ctgcgggcag
     1081 acctgctgcc gggaccagcg gcagtacgac gacttctgcc gcacgctgtt ctgcccggat
     1141 gcggtcctgt tctgccagca gcagaggctg tgccacggcc cggccggcgt ccgtcagctc
     1201 cactgccagc tgcaggttcg gagcgcgcag ggtacgcagc cagccggcat cctccagacg
     1261 acggcaggag gcgcgcaggt ttggcccgta gaccggggct tccccgcttt gctccagcac
     1321 ccgttctatg tcccgcgtca gcacgggtcc tggccgtttt tcatcaaggg cagccagcac
     1381 aatcagcact ctccgctgca gtggcgacgg tcggcggctc tgagtcattt ccagtcatcc
     1441 tccggccaga tgagctttct gaggcgctga tggtcggcgt caatgatctc ctgtctcttt
     1501 atggcattca ttgaggtata gccatttatg gccagttcat gccagaagat cagcacggct
     1561 ccggctttgg ctgactcgca gagataagtt acgccgtggc tgtccagatt gcattcccgg
     1621 atgcgttttt ccgcaaaccg ggtcagttgt tcatagctga gtgaaaacgt catttcctga
     1681 tccatcctgc acgttccatc tgataatgtt ctgacaggta aaaatca
//
LOCUS       DAZRVY010000299         1726 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZRVY010000299 DAZRVY010000000
VERSION     DAZRVY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.302"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..354)
                     /locus_tag="ACHXOX_004478"
     CDS             complement(<1..354)
                     /locus_tag="ACHXOX_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR7007750.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(354..848)
                     /locus_tag="ACHXOX_004479"
     CDS             complement(354..848)
                     /locus_tag="ACHXOX_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR7007751.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1206..1382
                     /locus_tag="ACHXOX_004480"
     CDS             1206..1382
                     /locus_tag="ACHXOX_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007752.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1524..1676)
                     /locus_tag="ACHXOX_004481"
     CDS             complement(1524..1676)
                     /locus_tag="ACHXOX_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007753.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1664..>1726)
                     /locus_tag="ACHXOX_004482"
     CDS             complement(1664..>1726)
                     /locus_tag="ACHXOX_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7007754.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          475 c          399 g          411 t
ORIGIN      
        1 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa taatcagcat
       61 atcagcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
      121 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
      181 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tccctgcccc
      241 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
      301 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
      361 tcctgctgta attcctcctc atcatccgca cctgcttcag tcacccgcag ggctatctga
      421 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccgcag
      481 tcacgttcaa gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
      541 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
      601 agacgctcac gttcagactg ataccaggct ttgcgctcat gcgcgtccat ttcgccttct
      661 gcaaccggcg gtggtaatgc cagaaatgcc gacacaatat caaccacccg ataaagcttg
      721 aggttgcttt catgcccccc tgcaacgggt agattttgca gccttgccgc agcagtctgg
      781 cgatgtacac ctgacagtgc cgccagttga ctgatattca gcgtcagatt ttttaactct
      841 cgatccatac ccgctccaga atgttttaaa catgcatctt gcgaacaact ttaggcaaac
      901 ggtgttagtg atgaacaaaa aacaatcaaa atcgacacca taaaaataaa accactgtaa
      961 tatcaatcca ttacagtagt ggtgatgacg aatgaaattt caaaaactag cctttttccg
     1021 cgccgctccc gccccgtggc aggccacccc accgggagga cccgtcagcc tgacagccat
     1081 gacgaacgtc tgatacagcg ccctgcatga atgacatcgg gataatccag aaaggaatag
     1141 catcgtaccc acaagaatca gtgtaagtgt cctgtttctt ccacccccgc acaggactgg
     1201 cgagcatgag ggacaaaccc gcgaaccata aacgcggtaa aaacccggtg tgcatcgttt
     1261 ttgattattc ccgcacactc gcgcagaagg agttccccgt cgggctacgg tcatggttaa
     1321 tacgggaata cggcgacgat acagcgcatg atgtgtcagg cttgaatacc tttatccgtt
     1381 aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcaa agccactctg
     1441 taggaagtgg cttttgtaat ggcaataaaa agccccgcga atgcgaggct aaatcctggt
     1501 atttgtaatg actggctctt atctcaacgc agccccttac cgcgcgcaaa atgctcaata
     1561 tcaagcatca gcaatgagat gtttaatctg gattcactcc agaagtgagc accaccctgt
     1621 ctacagagcc agatgtgaag gatgatgagt aaaattatcg ctatcatcga aggcattgcg
     1681 tcctgatgta ttcctgaagc gttctcagtg ctgtttggtc gcggat
//
LOCUS       DAZRVY010000300         1629 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZRVY010000300 DAZRVY010000000
VERSION     DAZRVY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.303"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHXOX_004483"
     CDS             <1..93
                     /locus_tag="ACHXOX_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7007755.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            104..292
                     /gene="dicB"
                     /locus_tag="ACHXOX_004484"
     CDS             104..292
                     /gene="dicB"
                     /locus_tag="ACHXOX_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR7007756.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="ACHXOX_004485"
     CDS             289..480
                     /locus_tag="ACHXOX_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR7007757.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="ACHXOX_004486"
     CDS             574..>1629
                     /locus_tag="ACHXOX_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR7007758.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAZRVY010000301         1617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZRVY010000301 DAZRVY010000000
VERSION     DAZRVY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.304"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            217..1260
                     /locus_tag="ACHXOX_004487"
     CDS             217..1260
                     /locus_tag="ACHXOX_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7007759.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          466 a          356 c          343 g          452 t
ORIGIN      
        1 caaaggaaaa gttgattcag atataattta gcatttcaat catctgacat cattataaaa
       61 aatgagattc acataaagat aaaattattg ttctcaagga aagcatgaag gggcttcgta
      121 agcaatattc attcggtgcc aagacaggac ttgatacaca gaatctcaac ttttatgctc
      181 tcagactata tcaccacatg ttttacaggc tcagaaatga acgatagatt atgctttgaa
      241 gtgcacgata acaagggtta tttcgtattt tcggatacct ggtttggccc attgctgggg
      301 gagtttgaag aagtgcttga tgcatacgac gctgatgaaa tcagcgagac gagttacatc
      361 aacaaactcc gacgccttgc tcaacgggag ccggacttta ttgatataca cgctcatctg
      421 gcatatgcat ttctggaaca gaatgccccg cgtaaggcac tgaacgcctc tcttaaagga
      481 ctggcggcag gtaatcgtat catcccggaa agtttctgtg gtgaaataat ctggatgcat
      541 cccgaaaacc ggccttatct gcgcgcgctt tatgcagcaa ttctcgcaaa tgtgcactta
      601 cagcgccatc aggatgcggt tatgctcact gataaaatac tggcatacaa tcctgaagat
      661 aatcaggggg ctcgctggtt gttgggttca gagctgttac gcaccggcga tcatgagcga
      721 gccttcagtg tactgaaaga gcatgctgat gaattttcac cttactggta tgaactcggt
      781 ctcctgcatt ttctgaacgg ggaacatgtt aaggccgcca cagcttttcg ccatggcttt
      841 gccacaaaca cttacatcgc agaaatgctg tgcggaaatc tgcatccatt tccacttgct
      901 gtgtggcatg acttctccgg ctctctggat acggcagaag attactatgc aacatactca
      961 ccattatggg gacaatatcc ggaagcattg ctgtttgtaa actggctcta taaccattcc
     1021 tccgtgctgc atgaacgatc agaaatcatt aaatgcgccg aaatgctgat tcaggaagat
     1081 gatttcgaaa tatgcgaaag tattttaaga cagcaggagc acctgtggaa aagaattgac
     1141 aagacacttt ctgaggaaat tgtacagaaa tgcagaaata tgaacggtga atatatctgg
     1201 ccctggatat tgcccttttc cgcagcaggg atgaaacatt caagcatcca gtatcagtag
     1261 atattgcatt agtgtaactt taaaacaagt aataatttat tatccaataa taacactgtg
     1321 ttccttcccc aggtgattac cctggggtta ttttctgaaa gtttcctttg caaaaggata
     1381 gcgttgtaaa ttactttaac gaattactcc atgaactcat taaagttctt tcgtaatact
     1441 ttatcgtcaa acacgttata gctggcatca aaaaccacag atttaccctg ccagcgggta
     1501 atcccctcct ctggcgtttt cggttgaact ggtatccccg tggatttgcc cctatatttc
     1561 cagacatctg ttatcactta acccattaca agcccgctgc cgcagatatt cccgtgg
//
LOCUS       DAZRVY010000302         1607 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZRVY010000302 DAZRVY010000000
VERSION     DAZRVY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.305"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..1607
                     /locus_tag="ACHXOX_004488"
                     /pseudo
     CDS             1..1607
                     /locus_tag="ACHXOX_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          497 a          314 c          333 g          463 t
ORIGIN      
        1 atcaaactgt cgaataaaat aggtcagcgt gtttatactg ttgtctctga aatgtcgatt
       61 gaagcatgga taacaaaaga accgcagcct tttgtttatc gccgtagcgg cgcttaccag
      121 aaactcacca ttggctcaga ctggggtcaa ctgtttgact gtgcctggat gcgagtttat
      181 ggtaaacgtc cagcagataa atttagacac aaactcgtcg ctctcgttga tatcggtggc
      241 gaaggtctta ttgtggataa gaatggttca cccatctgcg gcatcacgaa taaagcatca
      301 tcttatggcg taccgccaga taaaccaggt aaatgggtgg ttgacctttc tctcgttagt
      361 gaaaatgacg aggttgagtt ttggattgat gctgcctgca acgatttatt tggctatgtt
      421 actaatggag ggattattac tgatgttcat atcgcgacac gtaatcaact actgaaatcc
      481 ctttattatg atattgaggt gcttttcgac tggattaatg atggtcagaa gtttgaatcc
      541 atacatccta aaggtatcac cccagaacaa tttattgcca aaagaagtga gcgtactgac
      601 gaaatcataa gtactctcga atatattgat aatacgttaa ttacctttag caatgaagaa
      661 ataattaaat gtcaaattgc aatccaatcc ataatcaata aaaacaataa tccttcagag
      721 ttcaggatta tggctactgg tcatgcccat ttagatatag cgtggatgtg gcctttaaga
      781 gaaggacgca gaaaagcaat aagaacattt gccaccgccc ttgcaaacat tgaaaaatat
      841 cctgattaca tttttggcgc tagtcagtac caactttttc actggataaa aaaagattat
      901 ccctatttct tcgaaaagtt aaaaaaacaa atcgccgcag gccgctttga gttacaaggt
      961 tgcttctggg tcgaatgcga tttaaatctg gtaagtggtg aatcgctgat aaggcaaatc
     1021 atgcatggga cacgattcac actgcagaat ttttcaaaaa agataaatta tgtctggcaa
     1081 ccggatgtct ttggattccc cgcaacgctt ccacaaattc tcaaaaaatc aggcattaac
     1141 tatattgcat cgcaaaagct ctcacaaaac aagattaata aatttaataa ttaccttttc
     1201 cgctggcagg gcctggatgg ctcagaaatt ctgatgcata actttcctga agacacttat
     1261 gatagccgag cacgtgctcg ctctttagaa tatattgaac aaaactataa tgaaaaagag
     1321 atctgtccct atgcgcttat ggtgtatggt gtatggtgtc ggtgatggcg gcgctggccc
     1381 cggtgaggaa catatagaac gattaacacg tatacgcaat attgatggct tacctcatgt
     1441 tgatttcagc cgtgttgata agttcttcac ttacgccgac gcctttcgcg aaagcttgcc
     1501 cattatttca ggtgaacttt atttcgaggc tcatcaaggg tgctttacat cagagtcagc
     1561 aactaaagct cataatcgaa atatggaaaa taaattacat gatgctg
//
LOCUS       DAZRVY010000303         1605 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZRVY010000303 DAZRVY010000000
VERSION     DAZRVY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.306"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1398
                     /locus_tag="ACHXOX_004489"
     CDS             <1..1398
                     /locus_tag="ACHXOX_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR7007760.1"
                     /translation="NYISLPSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILF
                     CITTLYNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
BASE COUNT          443 a          336 c          298 g          528 t
ORIGIN      
        1 aactatatat ctctgccatc aataaaatcc agcactcaca ttgctctcct ttttatggtt
       61 tctatgggta cacaaaaatt aaaagctcaa agctttttta ttttcagttt attgctgacg
      121 ttaattttat tttgcattac taccttatat aacgaaaaca caaatgtaaa actcatccca
      181 cagatgaatt acctgatggt tgttgtggct ttgtttttcc ttaacgccgt catttttctt
      241 ttcatgttaa tgaaatattt cactaacaaa caaattttac caacactcat tttaagcctt
      301 gcatttttaa gtggccttat ctatttagtt gaaaccattg taattatcca taaaccaatt
      361 aacggcagta cactgatcca gacaaagtcg aatgatgttt ctattttcta tattttccgc
      421 caactcagtt ttatttgttt aacctcgctg gcactctttt gttatggaaa agacaacatc
      481 cttgacaaca ataagaaaaa aacgggaatc ctgttgctgg cgctgattcc ttttttagtt
      541 tttccccttc tggcacacaa tctgagcagt tataacgctg actattcttt gtatgtcgtc
      601 gactactgtc cggacaacca tactgcgacc tggggaatca actatacaaa aatattggtt
      661 tgtctgtggg catttttact gttctttatt atcatgcgta cacgattagc cagcgaacta
      721 tggccgttaa tagcattatt atgtctggca tcgctatgct gcaacttact tctactgact
      781 ctggatgagt ataattacac catctggtat atcagtcgcg ggattgaagt ttccagtaaa
      841 ctgtttgttg tgtcttttct gatttataac atttttcaga aactgcaact ctccagcaaa
      901 ctggcagttc atgatgtgct gaccaatatt tataatcggc gctacttttt caacagcgta
      961 gagtcattat tgtcgagacc tgttgttaag gacttctgtg tcatgctggt tgatattaat
     1021 cagttcaaac gcatcaatgc ccaatgggga catcgtgtgg gtgataaagt gctggtttca
     1081 attgtcgata ttatccagca aagcatccgc cccgatgata ttttagcgcg actggagggt
     1141 gaggtgtttg gcttgctatt taccgaactc aatagtgccc aggcaaaaat cattgcggaa
     1201 cgtatgcgta aaaatgtcga actcctgacc ggctttagta acagatatga tgttcctgaa
     1261 caaatgacca tcagtattgg cacggttttt tcaacgggtg acacgcgtaa tatctcgctt
     1321 gtcatgacgg aagcagataa agccttacgc gaagcgaaaa gcgagggggg caacaaagtg
     1381 attattcatc atatttaagt gcaaaaatat tcagagccat gctttttgcg tggcttttgc
     1441 atacaattta ttactattac ccctaaattt cccctcccca gcggcgcaga gatgagtata
     1501 attagcgccc ctgtgccagg ccgcaatcga actttatctg gttttctcgt ttcactaacc
     1561 gaaggagtgc catttatcat gaaattgcac catagaatgc tccgg
//
LOCUS       DAZRVY010000304         1587 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZRVY010000304 DAZRVY010000000
VERSION     DAZRVY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.307"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1587
                     /locus_tag="ACHXOX_004490"
     CDS             <1..>1587
                     /locus_tag="ACHXOX_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7007761.1"
                     /translation="AGVEKHIEQIYIDNEPVLAVPITAEGVVPKESIAAKYQNYLQLE
                     VRFGGDYTSTKTLAAKYAGSKWTNKFLGKGIVSISTVILKTQDSLENNILVNDQFALT
                     VEMKGQVIYDFVDGTYKASSNPPSIIYDYLTNSIYGMGIDPSLINTDTFAETAAYCDA
                     FEYYANGAISYQKTYKDNIENICQSFGGIMYVHAGQICITTDRKSVSVASFDERNMVG
                     AVQVSTSGGTDYFNVVDCKFTNPESMYTTDVVRIPSDITTDEAVQHDGQVIALSRDYS
                     WSYDQEVIAKMANVDVLKAKYALRTISFTTSEGWDLEVWDAINVSNEELAISGKFKVL
                     NKDISTDQENVGYVTITAVEAPDAMYDGIDPGIWSPGGVINFPELQVLPPTNLQAVRK
                     GNTTSGSIVDLTWDASPDPYLRGYYVYYRLNGSSTWTYAGQTGVQKLDYELFGLSDTA
                     SYDFSVEAYNNLGLVSKKLSLTGIVPTFNFALPAVTRVVLTNKTESAYVTDSPDFNIA
                     WDSQKDIRVNNRSFSEYFKY"
BASE COUNT          485 a          302 c          343 g          457 t
ORIGIN      
        1 atgctggtgt agaaaagcat attgagcaaa tctatattga taatgagcca gtacttgcag
       61 taccgattac agcagagggt gtagtaccta aagaaagtat tgcagcgaag taccagaact
      121 atttgcaatt agaagtacga tttggtggtg actatacgag tactaaaact cttgctgcaa
      181 aatatgctgg ttcgaaatgg acaaataaat ttcttggtaa gggtattgtt agtatcagta
      241 ccgttatctt aaaaactcaa gattcattag aaaataatat tcttgttaat gatcaattcg
      301 ctttaactgt agaaatgaaa gggcaggtga tttatgattt tgttgatggt acttataaag
      361 caagtagtaa ccctccttca attatctatg attacttaac aaattcaatc tatggtatgg
      421 gtattgatcc atcattgatt aataccgata cgtttgcaga aaccgcagca tattgtgatg
      481 cttttgaata ttatgctaat ggtgctatca gttatcaaaa aacctataaa gataatattg
      541 aaaatatctg tcagtcattt ggcggcatca tgtatgtaca tgccggacaa atctgtatta
      601 ccacagaccg caaatcggta tccgtagcct catttgatga gcgtaacatg gtaggggcgg
      661 tacaggtttc tacttctggc ggtactgact acttcaacgt agtcgattgc aagtttacta
      721 accctgaatc gatgtacacc actgacgtag tacgtatccc ttccgatatc actacggatg
      781 aagcagtaca gcatgatgga caggtcatag cattatcacg cgattactca tggtcatatg
      841 atcaagaggt gattgcaaaa atggctaacg ttgatgtact caaggctaag tatgccctgc
      901 gtactatcag cttcaccaca agtgaaggtt gggatctcga agtatgggat gcgatcaatg
      961 tcagcaatga agaactcgct atctcaggca aatttaaagt actcaataaa gatatcagca
     1021 ccgatcaaga gaacgttggc tatgtaacca tcacggcagt agaagcacct gatgcaatgt
     1081 atgatggtat tgatccgggt atctggtcgc ctggtggggt aattaacttc cctgaactac
     1141 aggtactacc gccaacgaac ctacaggcag tacgtaaggg gaatactact tctggttcaa
     1201 tcgtggatct tacctgggat gcttcaccag atccgtatct gcgtgggtac tatgtttact
     1261 accgtcttaa cggttccagt acctggacgt atgcggggca gactggcgta cagaagcttg
     1321 attatgaatt atttggattg tctgatactg caagctacga cttttcggta gaggcttaca
     1381 acaacttagg gctggtatcc aagaagcttt ccctaactgg cattgttcca acattcaact
     1441 ttgcactacc tgcggtaacg cgtgtagtac tgacaaataa aacagaatca gcgtatgtaa
     1501 ctgactcacc agatttcaat attgcctggg attcacaaaa agatatcaga gtgaataacc
     1561 gatcatttag tgagtacttc aagtatt
//
LOCUS       DAZRVY010000305         1536 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZRVY010000305 DAZRVY010000000
VERSION     DAZRVY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.308"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1536
                     /locus_tag="ACHXOX_004491"
     CDS             <1..>1536
                     /locus_tag="ACHXOX_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR7007762.1"
                     /translation="YEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDE
                     DDFKKPVRVNLPVVGDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTD
                     NTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTELHEARKNIPPK
                     NPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENKVRQTEDKQHQNSE
                     PEER"
BASE COUNT          439 a          400 c          372 g          325 t
ORIGIN      
        1 ttacgaagca aaaaatgaca aagtaagaaa acgcctcggt attaaaggcg gtttttactg
       61 ggctgaagcg aaaaaattat ccattgccat ctcccgtggt gctgttgcga ttgacgatgc
      121 tggctacgat gaagatgact ttaaaaaacc tgttcgcgtc aatttgcccg ttgttggtga
      181 cctcccacca gaaggcgtgt ttgatacgga attctgcaac cgttacgaaa aaggcgggga
      241 agatggcatc acaatggtat ttatcgcatc ttctccctct gttcaggaca aaccagccag
      301 cactgacaat accaacgtca acggcgaaga catgacagaa attgaggaga acatgctcct
      361 gccagtttcc ggtcaggaac tgcccattcg ctggcttgct caacacggca gcgaaaaacc
      421 ggtaacgcac gtttcacgcg acggactcca ggcattacac attgctcggg ctgaagaact
      481 accggctgtt actgccctgg ctgtttccca caaaaccagc ctgctcgacc cgctggaaat
      541 tcgcgatctc cacaaactgg ttcgtgacac tgacaaagtt ttccctaatc ctggtaattc
      601 aagcctggga ctgataactg cttttttcga agcatacctg aacgctgact acaccgatcg
      661 aggactgctg acaaaagagt ggatgaaggg taatcgtgtt tcacacatca ctcgcacggc
      721 ttccggtgct aatgctggcg gcggaaacct caccgatcgc ggcgaaggtt tcgtacacga
      781 tctgacgtca ctggcgcgcg acgtagccac tggcgtactg gcccgttcaa tggatctgga
      841 catctataac cttcatccgg cacacgctaa acgcattgag gaaattatcg ctgaaaataa
      901 accgcccttt tctgttttcc gcgacaaatt catcaccatg cctggcgggc tggattattc
      961 ccgcgccatc gtggttgcgt ccgtaaaaga agcaccaatt gggatcgagg tcatccccgc
     1021 gcacgtcact gaatatctga acaaggtact gactgaaacc gatcatgcca accctgatcc
     1081 ggaaatcgtg gatattgcct gcggtcgctc ctctgccccg atgccgcagc gtgtaacaga
     1141 agaaggaaaa caggatgatg aagaaaaacc gcaaccatct tgcgcaatgg cagatgaaca
     1201 ggcaacggct gaaacagtgg aaccggatgc aactgaacat catcaggaca cgcagccgct
     1261 ggatgctcag tcacaggtaa atgctgttga tgcgaaatat cagaaactgc ggacagaact
     1321 ccatgaagcc cggaaaaaca ttccgcccaa aaatcctgtc gatgcagaca aattactggc
     1381 tgcctctcgc ggagaatttg ttgaagggat tagcgacccg aatgattcga aatggattcc
     1441 cggtcatcat atctcctcaa atgaagtcaa aaaaacggaa aataaagtgc gacagactga
     1501 agataaacag caccagaaca gcgaaccgga ggagcg
//
LOCUS       DAZRVY010000306         1527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZRVY010000306 DAZRVY010000000
VERSION     DAZRVY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.309"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            71..514
                     /gene="cmtB"
                     /locus_tag="ACHXOX_004492"
     CDS             71..514
                     /gene="cmtB"
                     /locus_tag="ACHXOX_004492"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR7007763.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            542..>1527
                     /gene="cmtA"
                     /locus_tag="ACHXOX_004493"
     CDS             542..>1527
                     /gene="cmtA"
                     /locus_tag="ACHXOX_004493"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR7007764.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          355 a          339 c          388 g          445 t
ORIGIN      
        1 ttcattaaac cgcacgagta ttttattttt accgccggac ggtaccctaa aaataaaata
       61 aggataattt atgcggctta gtgactattt tccagaatca tcaatctcag ttatacattc
      121 agcaaaagat tggcaggaag ctatcgattt ctcgatggta tcattgctgg ataaaaacta
      181 tatcagcgag aattacattc aggcaattaa agattccacc attaacaatg gcccttatta
      241 tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg gaatgtgggg cgcttaaaac
      301 cgggatgtct ttgacattac ttgaacaagg tgtttatttt ccggggaatg acgaaccaat
      361 aaaattactc atcggccttt ctgctgccga tgccgattcg cacattggcg ctattcaggc
      421 gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa ctcttaacag cagcatcaga
      481 aaaacaatta gcggacataa tcagccgcgg ataattactc tctcctctac aggataatat
      541 tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt gggggctttt tgacggcaat
      601 ggtcatcccc aatattggtg cttttattgc ctggggtttt attactgcgt tatttattcc
      661 caccggttgg ctgcctaacg aacatttcgc caaaattgtc ggcccgatga ttacctattt
      721 attgcccgtg atgattggtt ctaccggtgg tcatctggtc ggcggtaaac gcggcgcggt
      781 catgggcgga ataggtacta ttggtgtgat cgtcggcgca gagatcccga tgttccttgg
      841 ctcaatgatt atggggccgc tcggtgggct ggtcataaaa tatgttgata aggcactgga
      901 aaaacgcata cctgccggtt ttgagatggt tatcaataac ttctcattag gtatcgcggg
      961 gatgctcctt tgtctgctgg gttttgaagt tatcggcccg gcggtgttaa ttgccaatac
     1021 tctcgtcaaa gagtgtattg aggcgctggt acatgcgggt tatctgcctc tgctgtcagt
     1081 catcaatgaa ccggcgaaag tgcttttcct caataatgcg atcgatcagg gcgtctatta
     1141 tccgctggga atgcaacagg cttcggttaa cggtaaatcc atcttcttta tggtggcgtc
     1201 caatcctggg cctggcttag ggctgctgct ggcgtttacc ttgtttggta aagggatgag
     1261 taaacgttct gcgcccgggg cgatgattat tcacttcctc ggtgggatcc acgaactgta
     1321 tttcccgtat gtgctgatga agccgctgac cattattgcc atgattgcgg gcggtatgtc
     1381 tggcacctgg gtgtttaact tactggacgg tggtctggtg gctggcccaa gtccggggtc
     1441 tatctttgct tacctggcac tgacgccgaa aggctcgttc ctggcgacaa ttgccggtgt
     1501 tacggtagct accctggtgt cctttgc
//
LOCUS       DAZRVY010000307         1525 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZRVY010000307 DAZRVY010000000
VERSION     DAZRVY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.310"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHXOX_004494"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHXOX_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHXOX_004495"
     CDS             complement(397..954)
                     /locus_tag="ACHXOX_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR7007765.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHXOX_004496"
     CDS             complement(951..1226)
                     /locus_tag="ACHXOX_004496"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR7007766.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHXOX_004497"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHXOX_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          324 c          367 g          410 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg tggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZRVY010000308         1485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZRVY010000308 DAZRVY010000000
VERSION     DAZRVY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.311"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHXOX_004498"
     CDS             <1..264
                     /locus_tag="ACHXOX_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7007767.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            275..712
                     /locus_tag="ACHXOX_004499"
                     /pseudo
     CDS             275..712
                     /locus_tag="ACHXOX_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1089
                     /locus_tag="ACHXOX_004500"
     CDS             712..1089
                     /locus_tag="ACHXOX_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007768.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          491 a          214 c          375 g          405 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aataattgag atatt
//
LOCUS       DAZRVY010000309         1482 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZRVY010000309 DAZRVY010000000
VERSION     DAZRVY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.312"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHXOX_004501"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHXOX_004501"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR7007769.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHXOX_004502"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHXOX_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          413 a          324 c          371 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZRVY010000310         1471 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZRVY010000310 DAZRVY010000000
VERSION     DAZRVY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.313"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1103
                     /locus_tag="ACHXOX_004503"
     CDS             <1..1103
                     /locus_tag="ACHXOX_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR7007770.1"
                     /translation="AWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFT
                     AQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTAT
                     HNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFL
                     AQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYV
                     TQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQ
                     VFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQ
                     VAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          359 a          331 c          390 g          391 t
ORIGIN      
        1 tggcgtgggc ggtctttaaa atctttcgta ttccggtgaa tcagtggacg ctggcgacgg
       61 cggcgctggg aggcgtgttt ctggtgagtg gtttgatttt gttgatgaac tacaaccacc
      121 cttacacttt taccgcgcaa aaggcagtga tagcgatccc tatcacgcca caggtgacgg
      181 gaattgttac tgaagtcact gacaagaata atcagcttat tcaaaagggc gaggtgcttt
      241 ttaagctcga cccggttcgt taccaggcgc gagttgacag acttcaggct gacctgatga
      301 cggcgacgca taatataaag acgctgcgtg cgcagctcac tgaagcgcag gccaacacca
      361 cccaggtttc agcggagcgc gaccgtctgt ttaaaaatta tcaacgttac ctgaaaggca
      421 gccaggcggc ggtgaatccg ttctcggaac gtgacatcga cgatgcgcgg caaaatttcc
      481 tcgcgcagga tgcgctggtg aaaggctcgg tggcggagca ggcgcagatc cagagccagc
      541 tcgacagtat ggttaacggc gagcaatcgc agattgtgag cttaagagcg caacttactg
      601 aagcaaaata taaccttgag cagactgtca ttcgcgcgcc gagcaatggc tacgttactc
      661 aggtactgat ccgcccaggt acatacgcag ctgccttgcc gctgcgtccg gtgatggtct
      721 tcatccccga gcaaaaacgg caaattgtcg cccaatttcg gcaaaactcg ctgttacgtc
      781 tgaaacctgg cgatgatgcg gaagtggtgt ttaacgcgct acctgggcag gtgttccacg
      841 gcaaattgac tagtatttta cctgtcgtgc caggcggttc ttatcaggcg cagggggtat
      901 tgcaatcatt aacggtcgtt cccggcacgg acggcgtgct gggaaccatt gaactggacc
      961 ctaacgatga tatcgatgcc ttacccgacg gcatttacgc ccaggtagca gtttactccg
     1021 accatttcag ccatgtttcg gtgatgcgga aggtgctgct aagaatgacc agttggatgc
     1081 attatttgta tttggatcat tgagggggaa gggggagatt atggaacttt gctccctgca
     1141 agggctaatt atttttgcat attacttcat tggctgcgat gatttttctt taatattcat
     1201 tatgttaata agttctggat gagtgtgctc tcattggatt ttttatccgt cttgttctgc
     1261 cgcaggatga gactatacat cgtcgagatc gcaatgatga tatgagttgt tcctcctgcg
     1321 acggctctaa tcaaaaaaga gcaggagtgc ttttccatct attgcgatga tggctttatt
     1381 atctgaagga tgacagtcac atttaaaaaa tatttaatct atagatcaca atgtagggtc
     1441 atggaatata tgtatacaac tattttcttc t
//
LOCUS       DAZRVY010000311         1443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZRVY010000311 DAZRVY010000000
VERSION     DAZRVY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.314"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHXOX_004504"
     CDS             <1..1117
                     /locus_tag="ACHXOX_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7007771.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..1334
                     /locus_tag="ACHXOX_004505"
     CDS             1095..1334
                     /locus_tag="ACHXOX_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007772.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGAPIKLSSML"
     gene            complement(1329..>1443)
                     /locus_tag="ACHXOX_004506"
     CDS             complement(1329..>1443)
                     /locus_tag="ACHXOX_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007773.1"
                     /translation="MSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          386 a          302 c          439 g          316 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga gggtgcccct attaagttgt
     1321 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     1381 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctgactc
     1441 att
//
LOCUS       DAZRVY010000312         1434 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZRVY010000312 DAZRVY010000000
VERSION     DAZRVY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.315"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHXOX_004507"
     CDS             complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHXOX_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR7007774.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            233..904
                     /gene="queE"
                     /locus_tag="ACHXOX_004508"
     CDS             233..904
                     /gene="queE"
                     /locus_tag="ACHXOX_004508"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR7007775.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          416 a          277 c          303 g          438 t
ORIGIN      
        1 cgttatcatt aaaaactaat acagaaaaaa taaatataat tttggtgaat gttttgacat
       61 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
      121 cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata agacatgatt
      181 gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
      241 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
      301 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
      361 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
      421 gagtgataag tggggggcgg cgagcagtga agacttgttg gctgtcatta gtcgccaggg
      481 atacaccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctacc
      541 actgactgat ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggtactca
      601 tgaggtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
      661 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
      721 gcgcgtacgc gatattgaag cactggatga actattggca acgctgaccg atgataaacc
      781 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
      841 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
      901 ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt ctactcattt
      961 atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta atcattagca
     1021 ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg cggtaaagtt
     1081 tggtagattt taatttgtat agagttattt taaatattta cctttttaat caatgaatta
     1141 agtactcttt aacataatgg atgtgttgtt tgtgtgatac tgtaaagttg gtagattgtg
     1201 actggcttaa aaaatcatta attaataata ggttatgttt agagtgttcc ccgcgccagc
     1261 ggggataaac caccatataa cccgttatct ctttctcaag tttttatatt agcagtactt
     1321 gtaataagca aaatatccac gtaacacctc atgttcaaaa tagttctcca tgccagagag
     1381 gttcacaatt atcgatacaa aaaattaaat ttaatcaaag tgttatttgt atga
//
LOCUS       DAZRVY010000313         1426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZRVY010000313 DAZRVY010000000
VERSION     DAZRVY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.316"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..659)
                     /locus_tag="ACHXOX_004509"
     CDS             complement(<1..659)
                     /locus_tag="ACHXOX_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFR7007776.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
     gene            complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHXOX_004510"
     CDS             complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHXOX_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR7007777.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHXOX_004511"
     CDS             complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHXOX_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR7007778.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1202..1342)
                     /locus_tag="ACHXOX_004512"
     CDS             complement(1202..1342)
                     /locus_tag="ACHXOX_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR7007779.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          379 a          349 c          332 g          366 t
ORIGIN      
        1 ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat
       61 tcaccgtcct gcaattcact ccacactgct ttgccgagat attcgaggtt aacctgatgg
      121 caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc
      181 aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc
      241 tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag
      301 agcgggttaa cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg cattgccgcc
      361 agatcaacaa cggcaggtac gccggtaaag tcctgcatca acacccttgc cgggcggtag
      421 gcaatttcac ggtcagcatg ggcatttttc agccatcctg ccagcgcgtg gatatcctct
      481 tcggtaaccg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac tttgagtgac
      541 ttgggtagac gggtgatatc gcccagtgat ttagcagcaa gcggcaggct gtagtagtgg
      601 taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt tgacgacata
      661 gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa tattaaagat
      721 aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag gagaaacctt
      781 gtggcaaagc aaatgacaac cccgccgcag cggggtcaag gatcagtgag taatgatcca
      841 gaggagttcg gcccagaaga gcatcgacag agaaaacgcg cccatccagg accaatattt
      901 catatttgtg ttgatcatag gctacaccct taacggttat tattttgtgt tgagataact
      961 caagctgtta ttgctgcggt gtattaaaag ccctttaatt cattaaaaat gggtaataag
     1021 aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat aaaggcttgc
     1081 tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg tttcgtttct
     1141 gtttcattgt cactatgctg gcataatggt gacgactcca gttgctcctg accgaccata
     1201 gttatcccca cactttccaa atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa
     1261 gaaaaaccgc cagccaggct ttacgtttta attcaggatc gcggcggggt tcctgatttc
     1321 cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg ttgaacgatt
     1381 ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagt
//
LOCUS       DAZRVY010000314         1426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZRVY010000314 DAZRVY010000000
VERSION     DAZRVY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.317"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXOX_004513"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXOX_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR7007780.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXOX_004514"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXOX_004514"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR7007781.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHXOX_004515"
     CDS             complement(822..>1426)
                     /locus_tag="ACHXOX_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR7007782.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZRVY010000315         1421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZRVY010000315 DAZRVY010000000
VERSION     DAZRVY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.318"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHXOX_004516"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHXOX_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR7007783.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZRVY010000316         1420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZRVY010000316 DAZRVY010000000
VERSION     DAZRVY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.319"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            461..916
                     /gene="csgB"
                     /locus_tag="ACHXOX_004517"
     CDS             461..916
                     /gene="csgB"
                     /locus_tag="ACHXOX_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR7007784.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            957..1409
                     /gene="csgA"
                     /locus_tag="ACHXOX_004518"
     CDS             957..1409
                     /gene="csgA"
                     /locus_tag="ACHXOX_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR7007785.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          432 a          273 c          330 g          385 t
ORIGIN      
        1 ttaaaaaatc atacaaatgg tgataactta ctaataatgc atataaaaaa tatttcggtg
       61 tagtcctttc gtcatgtaaa aagttgttgt tttttctcca cacctccgtg gacaaatttt
      121 tactccaaaa agacgaggtt tgtcacggct tgtgcgcaag acatatcgca gcaatcagcg
      181 atgggcaaga agaatgactg tctggtgatt tttgatagcg gaaacggaga tttaaaagaa
      241 aacaaaatat tttttgcgta gataacagcg tatttacgtg gcttttaata ctttggtatg
      301 aacaaaaaaa gaaaaataca acgcgcgggt gagttattaa aaatatttcc gcagacatac
      361 tttccatcgt aacgcagcgt taacaaaata caggttgcgt taacaaccaa gttgaaatga
      421 tttaatttct taaatgtacg accaggtcca gggtgacaac atgaaaaaca aattgttatt
      481 tatgatgtta acaatactgg gtgcgcctgg gattgcagcc gcagcaggtt atgatttagc
      541 taattcagaa tataacttcg cggtaaatga attgagtaag tcttcattta atcaggcagc
      601 cataattggt caagctggga ctaataatag tactcagtta cggcagggag gctcaaaact
      661 tttggcggtt gttgcgcaag aaggtagtag caaccgggca aagattgacc agacaggaga
      721 ttataacctt gcatatattg atcaggcggg cagtgccaac gatgccagta tttcgcaagg
      781 tgcttatggt aatactgcga tgattatcca gaaaggttct ggtaataaag caaatattac
      841 acagtatggt actcaaaaaa cggcaattgt agtgcagaga cagtcgcaaa tggctattcg
      901 cgtgacacaa cgttaatttc cattcgactt ttaaatcaat ccgatggggg ttttacatga
      961 aacttttaaa agtagcagca attgcagcaa tcgtattctc cggtagcgct ctggcaggtg
     1021 ttgttcctca gtacggcggc ggtaaccatg gtggtggcgg taataatagc ggcccaaatt
     1081 ctgagctgaa catttaccag tacggtggcg gtaactctgc acttgctctg caaactgatg
     1141 cccgtaactc tgacttgact attacccagc atggcggcgg taatggtgca gatgttggtc
     1201 agggctcgga tgacagctca atcgatctga cccaacgtgg cttcggtaac agcgctactc
     1261 ttgatcagtg gaacggcaaa aattctgaaa tgacggttaa acagttcggt ggtggcaacg
     1321 gtgctgcagt tgaccagact gcatctaact cctccgtcaa cgtgactcag gttggctttg
     1381 gtaacaacgc gaccgctcat cagtactaat acatcatttg
//
LOCUS       DAZRVY010000317         1403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZRVY010000317 DAZRVY010000000
VERSION     DAZRVY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.320"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHXOX_004519"
     CDS             complement(<1..185)
                     /locus_tag="ACHXOX_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR7007786.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHXOX_004520"
     CDS             complement(230..1216)
                     /locus_tag="ACHXOX_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR7007787.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          390 a          303 c          284 g          426 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1381 gtttataatc aataaatgat gag
//
LOCUS       DAZRVY010000318         1392 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZRVY010000318 DAZRVY010000000
VERSION     DAZRVY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.321"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHXOX_004521"
     CDS             <1..459
                     /locus_tag="ACHXOX_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7007788.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHXOX_004522"
     CDS             477..1115
                     /locus_tag="ACHXOX_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7007789.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZRVY010000319         1384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZRVY010000319 DAZRVY010000000
VERSION     DAZRVY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.322"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHXOX_004523"
     CDS             complement(<1..1339)
                     /locus_tag="ACHXOX_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR7007790.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZRVY010000320         1377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZRVY010000320 DAZRVY010000000
VERSION     DAZRVY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.323"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHXOX_004524"
     CDS             <1..984
                     /locus_tag="ACHXOX_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7007791.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHXOX_004525"
     CDS             999..>1377
                     /locus_tag="ACHXOX_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR7007792.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZRVY010000321         1364 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZRVY010000321 DAZRVY010000000
VERSION     DAZRVY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.324"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..1259)
                     /locus_tag="ACHXOX_004526"
     CDS             complement(<1..1259)
                     /locus_tag="ACHXOX_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR7007793.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          379 a          348 c          305 g          332 t
ORIGIN      
        1 gtgtaaccta ttgcgttgtt tgtattacgg tattcggcaa cgactttaat cattccctcc
       61 atcatacttg ccacttctgt ttcctgcggc gagatcatgc ggacattttt catgacctgg
      121 gattgcatca ccgtctggct accagagtct tccgggcgtt gccaggtctg gatctcctga
      181 tcgttaccgc caacagtacg ccagttggta attgcaccac tgaagatgtc acgcacctgt
      241 tgttcagtca gggaattaac cggattatcc gcattgacga tgaaaacaaa cgcttcacgg
      301 gcaaaaggcg tgtacagcaa agtaacgccc gattcctcag cgcgtttttt ctgcccaccg
      361 gaaggttgcg ccacaaaaat aatatcagca tcacctttaa caatcctgtt atacgcatcg
      421 ggggtacgag agctctccag atattccctg gtgtgaaaat cttccggtat aacgcttaaa
      481 gcataaaatg cagaggcata aattgggtac gccgccgtag caccatcgag tcgcggccag
      541 ttttgcgtaa actgaatttg cggtttacca cgcagtggtg tcagttgatt attgagttta
      601 tcgggtcgcc aggcccaggt atcaatattt tcgcgcacac cgacaccggg attgagctgt
      661 ttatataaat aaccctgata accagcgaca aaaccaagca tgagaaggat aaacaggagg
      721 gaattacggc aacgtagccc ggatgtaccg gttaaaggat gtctggttgg ccggccgtac
      781 cccagcgaaa aactgatatg cgcgccaacg ggtataatga ctatccacca gtactcacct
      841 ccgactactg agactaacaa aacgcccagc caggggagca taagatcagc caaccaaccc
      901 gatcccatct ggtgaaattg ataatctgcc agatgcagcg cgattatcca gccaaccagg
      961 attaataaac cgggtactaa aaaaagacat tgtcgccaga cggctatttc tcttttttct
     1021 ggtgcactat cgcctgccca tactcgcccc gagaaaaata gcattaatgc gcccagcgcc
     1081 agcataaaaa tgttgagcag aaggtaactt gttggcgtga taaggatatt acaaacttca
     1141 ataataccgg caagtccgac acccagcata gcaacaaccg ccgccaaaaa ttgcgtcagt
     1201 atggggatgc cgaaaaaagt cattaccagc gaggacaaaa tccattttct gttttgcatt
     1261 attctttcca ttctttttga atggtgaaat tatactcccc gagtcccctt gccccttctg
     1321 gacacttttc cgaaatgatg gcggaaaaaa acgggaccct ttgg
//
LOCUS       DAZRVY010000322         1343 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZRVY010000322 DAZRVY010000000
VERSION     DAZRVY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.325"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            143..1117
                     /gene="cysB"
                     /locus_tag="ACHXOX_004527"
     CDS             143..1117
                     /gene="cysB"
                     /locus_tag="ACHXOX_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR7007794.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          356 a          308 c          298 g          381 t
ORIGIN      
        1 tttttcttca cacctataca ctaaggctat aaatgatata gtggttatag ttagcacctt
       61 ttttattatt aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg
      121 gcgtctaagt ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt
      181 caatcataac ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat
      241 cagtaaacaa gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg
      301 caagcacctg acgcaggtaa cgccagcagg gcaagaaata attcgtatcg ctcgcgaagt
      361 cctgtcgaaa gtcgatgcca taaaatcggt tgccggagag cacacctggc cggataaagg
      421 ctcactgtat atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa
      481 aggctttatt gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca
      541 aattgctgat gccgtctcta aaggcaatgc tgatttcgct atcgccacag aagcgctgca
      601 tctgtatgaa gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac
      661 tccggatcac ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc
      721 gttggtgaca tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg
      781 cgcagggtta acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta
      841 cgtccggtta gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga
      901 tcccgacctt gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg
      961 ttttcgccgt agtactttct tgcgcagtta tatgtatgat ttcattcagc gttttgcacc
     1021 gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga
     1081 ggtcatgttt aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc
     1141 ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt acttatttct ataccgtgca
     1201 caatattttt atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc
     1261 cgcctcgcaa actccagttt tcaaatatct atttcaggtc aaaagattga attattcact
     1321 ggagacgatt cgtaaattcg ctg
//
LOCUS       DAZRVY010000323         1341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZRVY010000323 DAZRVY010000000
VERSION     DAZRVY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.326"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHXOX_004528"
     CDS             <1..184
                     /locus_tag="ACHXOX_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007795.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXOX_004529"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXOX_004529"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR7007796.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZRVY010000324         1327 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZRVY010000324 DAZRVY010000000
VERSION     DAZRVY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.327"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..1311
                     /gene="espX1"
                     /locus_tag="ACHXOX_004530"
                     /pseudo
     CDS             1..1311
                     /gene="espX1"
                     /locus_tag="ACHXOX_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          508 a          230 c          192 g          397 t
ORIGIN      
        1 gtataaaaca gagttcttaa ttttgtttga tgactttttt agaagccata acttgcctaa
       61 accatctcca gtccttcatc atttttttca atatactcat ctgagagatg catattttta
      121 tagatgtaaa ctcatagaac atacagttca attttatttt ttaaacacaa aggtatcaca
      181 ttacttagat tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag
      241 atataccaag aatgccatga aaaattcgtt aattttttaa aggcaaactt caaccaagaa
      301 atatacccac agctatatac tcctgaaata ttttatgagg cctgcagaaa tctccagtca
      361 ttttatgatc atcaggaaac gtcacaaaac gcaaaataca gtgcaataga caagaaaaaa
      421 agtcatctta accaggaaat caaaaagctt attaaaaaaa atattttccc tgagctatac
      481 aatgagcaat gcaataaaat cccctcctca tcgatagatg ataaccaggg aataatgtgg
      541 cagaacttta aaactagcaa ttcggcatat tcaaaactat gtggaaaatt atctttattt
      601 aaaagcgtgc ccagtcggtt aatagaaaaa tcagcttact gctcaactga aaacatggcc
      661 actaataaat ttaatgtcgt cttttcttac tgcggagata atataaagga atttatactt
      721 ttattaccct ataataaatc actggagatg tatgaactta acgatcaaaa gatccagtat
      781 ttgactgccc ttaatattaa tatacataaa atgttgctat caaatataac aatagaaaag
      841 agcaaccttt catatgggta ttatttttgt tgtgtattaa ccaatatatc atgttttgaa
      901 agcgacttat caaacaccat cttttcaaat ggagaaataa acaacctctt cattaaaaaa
      961 agcaacatat ttggaacatc gttcactaat accatgataa aaaatttgcg ctgtgaagat
     1021 attatgcctg gccgatggac cactcaactt gtcaataagc acttaggtta cagatacaca
     1081 ggagtattca aaaccctggc aagtattgat gacaaaccgt cccgtttcga aattttgata
     1141 ccattggtac aaactttagt tcgcgataat gtaaagctaa ataatgatgt gtataaagaa
     1201 cttaataagt ttatgcacga ttacgacaaa acatcatcaa aaatgagaaa ataccttcag
     1261 agcatcaacg agtgcatgct ccttataaaa aatatagttc atcaggatta aaacactcaa
     1321 agaaaga
//
LOCUS       DAZRVY010000325         1302 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZRVY010000325 DAZRVY010000000
VERSION     DAZRVY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.328"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHXOX_004531"
     CDS             complement(78..1022)
                     /gene="leuO"
                     /locus_tag="ACHXOX_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR7007797.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          403 a          292 c          255 g          352 t
ORIGIN      
        1 attcagaata aaattctaca ttcaataaaa aatacagaat aaaccagaca ttcatgtctg
       61 acctattctg cattcagtta gcgtttgcaa attgagacta attgctcttc catccactga
      121 tggcctttat cgcgcccggc agcttcatgc caggagagat aacaggttct gctattttgt
      181 tttaacggca gcggtaatac ctgtaattct aaggattcag cgaactcttc agccagccaa
      241 cgcggcgcaa tagcgaccaa atgcgtttgc gacaccacgc taagtacgct catcattgcc
      301 atgccctgat acgcgatact ggcttgctta tctaccgtgt cataccaagg ttgactaaat
      361 gacgcgaaac gatcgagcga aaccgccgca tgttgttcgt tataaacatc atgtttcagt
      421 aacgggccct taattgttgg atgatttttg ctggctacca gcaccatttc atctttaaat
      481 aatggcacgc tggtaaattc aggacgatgg aagtcttcat aactaatcac aaactccgtt
      541 tcctgataac gcagctgatg ttcagtgttc tgatttaatg aagacttgaa cataacatgt
      601 atatttggcg caatctgctc aatgtgatta taaatctgcg aggtcagaat gctgtctaac
      661 gggctgcaaa cacaaagatg aaatacacgt tcactgctcg cgggttcaaa acctgaacca
      721 ggcaattcat tttgtactag ttgcaatgcc tgacgaactg agccaaaaag ttgaaatgcg
      781 cgagcagtcg gttgaatacc acggccataa cgaacaaaaa gctcgtcatt aaacatcacc
      841 ttcaggcgtg caacagcgtt actgaccgca ggttgcgaca tacccagaac atgagcggca
      901 cgggtaatgt tttgctcctg catcacggca tcgaaaacgg ttaataagtt gagatcgacc
      961 atgcgtagct gtggcttgct taactccgcc gtctctgaat gatctgtttg tacctctggc
     1021 atacttaact ccactgtcac acttaactcc ctttccctta ttggaatgca gagaaataat
     1081 cctgaagaat atgatttatc atgcatataa aataagaaaa agcaaaatga gtaaaattcg
     1141 gaaaacataa agatacgggt tctgataaaa acagaatcca ttgccgtgga atgagtcatt
     1201 taccacagca taagaatcca taatgttttt gttgcgaaaa caatctaatc ataactaccg
     1261 cgaatactca atcatctaca aaatggatta aatgtgctta tt
//
LOCUS       DAZRVY010000326         1278 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZRVY010000326 DAZRVY010000000
VERSION     DAZRVY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.329"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHXOX_004532"
     CDS             <1..69
                     /locus_tag="ACHXOX_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007798.1"
                     /translation="RRRCDVLFAMMRDGTFYTPQGS"
     gene            complement(94..579)
                     /gene="umuC"
                     /locus_tag="ACHXOX_004533"
                     /pseudo
     CDS             complement(94..579)
                     /gene="umuC"
                     /locus_tag="ACHXOX_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(579..998)
                     /locus_tag="ACHXOX_004534"
     CDS             complement(579..998)
                     /locus_tag="ACHXOX_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR7007799.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          359 a          332 c          270 g          317 t
ORIGIN      
        1 agacgacgct gcgacgttct gttcgccatg atgcgcgacg ggacttttta taccccgcag
       61 gggtcataac atgcttgaca acttaatagg ggcacccccc gtctgccgct gccatttttt
      121 tgccgcatga ttggcaagct tagccagcgt tttggtctgg gcgatcccca taccaacagt
      181 aagatgggta cgttgtagca ccgttgcgcg aatttctctg ccaaaatcag tcagatcgcg
      241 acaattacgc acacctgtca gatcgcagaa tgcctcatca atactgtaaa tctcgacgcg
      301 gggcgatagt tcttccagcg tcgacatcac ccgattgctc atgtctgcgt aaagctcata
      361 attgctgcta aagcaaacca caccacagcg acgaaacaga tctttttgtt tgaaccaggg
      421 atcgcccatt ttaacaccca gcgcctttgc ctcagcgttt cgggcgataa cgcaaccgtc
      481 attattcgat agcacaacca ccggtttacc ccataaatca gggcgaaaca ccgtctcaca
      541 gctggcataa aacgcgttta catcacagag agcaaacatc agcgcatcgc cttaacgacg
      601 tggatcacca caccaaagac atccagcgta tcttcgctac tgatggtaat gggcgagtac
      661 gcactgttca tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt caccgtaaac
      721 tcgccgtcaa cagcagcgat gacaatatca ccatggctgg cggtaatagc gctatcgaca
      781 atcagtaaat caccgtcact aattccacca tcaatcatag aatcaccact tgctttgacg
      841 aagtaagtcg cgctgggatg ctggatcaac agttgattca gatcgatgcg ctgttcgacg
      901 taatctgctg ccggtgaagg aaagccacac tgaacaagat cgctaaatag cggaaaagtc
      961 acaatttcgc ggagatccgc aggcttgata aacaacataa taatctgcct gaagttatac
     1021 tgtttttata tacagtaatc tgttcttgcc agcagatcaa tactgattca ggctatcaat
     1081 atttgtcgct gcatagactg ctgatttttc gttctcttat cctgtgctca cgtggccttc
     1141 tggcgacgac gctcatccag cagaaatgaa aaatatcacc cggcaaaaaa atagaataga
     1201 agcattcgcc ataatgacat taaacattgt ttgatattta tcatattaat agaaataaag
     1261 acattgacgc atcccgcc
//
LOCUS       DAZRVY010000327         1229 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZRVY010000327 DAZRVY010000000
VERSION     DAZRVY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.330"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..990)
                     /locus_tag="ACHXOX_004535"
     CDS             complement(<1..990)
                     /locus_tag="ACHXOX_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR7007800.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLK"
     gene            complement(1007..>1229)
                     /locus_tag="ACHXOX_004536"
     CDS             complement(1007..>1229)
                     /locus_tag="ACHXOX_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFR7007801.1"
                     /translation="YVGPTKPWHYWAGYPSAQPFINAKEASPWKNEPFMRPINSNYAR
                     YCAKHNFKQNEPINGIMNYIYYFYLKIIK"
BASE COUNT          385 a          223 c          218 g          403 t
ORIGIN      
        1 cttaagcttg tacttaatta atgaagttat gcctttaata tactcaccat gggcaaacag
       61 atgcttatat tgtttctgca tttctgcaac agtccgcgcc tctttaagag gatatttctt
      121 ccagggagat tgttcacgcg cgatattaaa gtatgatgca gacggataac cagcccagct
      181 atgccatggt ttagttatcc ctgtatagtg aatcaatact gttttatcgg taatggtttg
      241 ctgatacttt cgatgactac gatcatgaag ttcgtttttc agggtataaa tagtatcaaa
      301 atccttcgcg aggtagatat tattcatatt aaatgcgata ttcaacgcat cctgatctaa
      361 atatttaaga gagccatatt tagtttcccc tcgtaaaagc gtgagtaaat acggtgtcaa
      421 atttgcttca tgccattttt taagattgac atagataacg cctgcattga aataacgtcc
      481 attcatctcg ggaatgttga ggcgttttgc acatgccgcc tgagtactaa cattgtcagg
      541 aatgaccgca gcaaactcat ctttaaatat aatttctgtg agaggcttca gacttccttt
      601 acaaacgaca tcggcatcca gatacagcag tgtggaaata ctttcactca gatattcaaa
      661 ggacaatacc ctgaagtatg tcgcgtacga ccaaaactgt gacgtcggca gatcagcaaa
      721 gtattttggg tcaattaaat atacaataat tgaggtatta aattgttttg ctgtttcatt
      781 aaatgacttt aaataatcat catccacata atcagtgaaa acatgaaaga caaaattaat
      841 atctgaatta ttaatcaaaa cggaagaaat tgaaacgcca gcaccataca aaaaattctt
      901 atcgataccg taggaaacat ttagttctgg ggcgttttct tttttattat ctgctaaata
      961 cgaaaaccgt tcttttataa attcattcat ttaacttttc tcaatatcat tttattatct
     1021 ttaaataaaa ataataaata taattcatta tcccgttaat tggttcattt tgtttaaaat
     1081 tatgcttggc gcaataacga gcatagtttg aattaattgg ccgcataaac ggttcatttt
     1141 tccagggcga tgcttctttg gcgttgataa aaggttgcgc acttggataa ccggcccagt
     1201 aatgccaggg ttttgtcggg ccgacataa
//
LOCUS       DAZRVY010000328         1208 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZRVY010000328 DAZRVY010000000
VERSION     DAZRVY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.331"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>1208)
                     /locus_tag="ACHXOX_004537"
     CDS             complement(<1..>1208)
                     /locus_tag="ACHXOX_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007802.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQW
                     QYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYS
                     EQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYE
                     LATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHT
                     TAANLDIDIP"
BASE COUNT          182 a          415 c          340 g          271 t
ORIGIN      
        1 tacgggatat cgatatccag gttcgctgcc gtggtgtgga cgcccgtcag cctccccgtg
       61 tcgctgtaac ggtactcccg gctctcctgc gggccactga tgcggattag ctgtccgttg
      121 tcgttccagt cgtagtcacg gtcaagctgc ggaaggttca ggtgcctgct ccggagctgg
      181 ccgctggtat tccaggcggt ggcaagttca tatgcctccc cgccgaagct gcgggccgtc
      241 tcacggtgca gccggtcccg cgtgtactcg accagcggtg ttccgcccag cttcatcccc
      301 gcaagataac cgctgccata cgtcagccac tccaccggcg gcaggccgtc cggctcctga
      361 cgggtcgcca gcccctgttc gctgtacgcg tgccccgtct catgctccca cagcatctcc
      421 cccgtctccg gggtctccac cgtctgccgt tcgcccgtca gacggccttt atcgtcatag
      481 ccgtagtgga ccgccacacg gtggccttca ctcaggtggc tgatttctgt cagccagccg
      541 tggtcgtcat actgccactg ctccgccggt tcaccgttca ccgtgcgccg ggtgatacgg
      601 tccgacgcat cgtaatgcca gagggtgata agcccctcat cttcactctg cgtgagtttt
      661 ccggtcaggt catactggta acgttgcgtc cggccgtcaa agccgcgctg ctccgtcagc
      721 ctgtcgaccg ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg
      781 atgcgcccgg cggcgtcata ccccatgctt cgcgtcagac cgccctgcgt ggtgctgacg
      841 gcctttcccc acgcatcata ctgtatctca ctgcggctgc cgtccggggc aacgatagcg
      901 gtgaggtctc ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccctctga
      961 ctgaccagtt gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga
     1021 acggcgattt gttgaccgta ccggtcatac tcataccgcg tcgtgtaccc cgagcagtcc
     1081 gtaaacgcct gaagctgacc gtaccggttt cgcgttattc tccgcctcac cccggttccg
     1141 tcttccaccg cacagggcag gccactgcgg gaagaatcat aaaaccagcg cgtaatgtta
     1201 ccgttgcg
//
LOCUS       DAZRVY010000329         1170 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZRVY010000329 DAZRVY010000000
VERSION     DAZRVY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.332"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHXOX_004538"
     CDS             <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHXOX_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR7007803.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          295 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg
//
LOCUS       DAZRVY010000330         1166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZRVY010000330 DAZRVY010000000
VERSION     DAZRVY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.333"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            135..653
                     /locus_tag="ACHXOX_004539"
     CDS             135..653
                     /locus_tag="ACHXOX_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR7007804.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            863..>1166
                     /locus_tag="ACHXOX_004540"
     CDS             863..>1166
                     /locus_tag="ACHXOX_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR7007805.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          276 a          301 c          307 g          282 t
ORIGIN      
        1 aattctagct cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag
       61 ctgcatctgc ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa
      121 caaggagttt acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa
      181 atattactgc aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg
      241 aagacgagat gctggtgcag gaattcctgc ataacgttac tgtcccgacc actcctcagt
      301 ccggtcagcc tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca
      361 aagcggttcc gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac
      421 ttcactggtg gcgtacctct gtggagggca aacaggagca ttatttcact acccgcctga
      481 ctgattcgac gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac
      541 gcgagttcac ccagttgctc gaggtttcac tggcctaccg caaaattgaa tgggaacacg
      601 tcaaatctgg cacctccggt gccgacgact ggcgcgcacc actggaagca taagttaagc
      661 caacagcatc cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt
      721 tgtcagtaag gggttacgtt cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt
      781 gcctgaacgc aagcgtaaca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt
      841 ttccttaacc ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg
      901 cctgccaccg gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct
      961 cttttccctt gagctctctc tggtcagcca gcagtttctc tcccttgaat ttccgcaggt
     1021 actggacaaa atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa
     1081 aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagatgc tgtacagcat
     1141 gaaggtatgc ccaccgctgt ggcgca
//
LOCUS       DAZRVY010000331         1166 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZRVY010000331 DAZRVY010000000
VERSION     DAZRVY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.334"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1166
                     /gene="istA"
                     /locus_tag="ACHXOX_004541"
     CDS             <1..>1166
                     /gene="istA"
                     /locus_tag="ACHXOX_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR7007806.1"
                     /translation="WMEYKAQAGDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLF
                     IDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFL
                     GGVPKLLIPDNLRSAVKKADRYEPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENG
                     VLIVERWLLARIRNETFHTLRALNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPA
                     LSPLPLEPYEYTEYKAVKVGPDYHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKG
                     QCVAQHPRSTHEYKHTTNPLHMPERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKA
                     KPHPELAYRAVLGLLALQKKYGPERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          292 a          334 c          314 g          226 t
ORIGIN      
        1 gtggatggaa tacaaggcac aggcgggtga caaagcgatg ggatactcac acttctgccg
       61 ttgctaccgg aagtggaaaa aaacgcgacg cctctccatg cgacaggagc atcgtgctgg
      121 cgaaaagctc tttatcgact tctgtggtcc tacggtccct gttattaatc cggacaccgg
      181 agagatacgc cgcgtggcaa tcttcgtggc tgtaatgggg gcgtccaact atacctatgt
      241 tgaagcgtgc gaaggtcagg acatgatgtc atggttaaat gcccacagcc gttgcctgac
      301 cttcctgggc ggcgtaccga agctgctcat ccccgataac ctcagaagcg cagtgaaaaa
      361 agccgatcgc tatgagcctg tcatcaacga cagttatcag gcgctggctg agcactatgg
      421 cacggttatt atcccggccc gcccgcgcaa acctaaagat aaaccaaaag cagaaaacgg
      481 cgtcctcatc gtcgagcgat ggcttcttgc ccgcatccgt aatgaaacct tccacacgct
      541 cagggcgctg aatgcacgcc ttcgggagtt gctgacggac atgaataacc gaccaatgaa
      601 gggatacggt aatcaaaccc gcgcagaacg cttccgcatg ctggatgcac cagcactctc
      661 tccgctgcct ctggagcctt atgaatacac ggaatacaaa gccgtgaagg ttggccctga
      721 ctaccacgtt gaatatgccc gtcactggta ctccgttcca cacgaactgg tcggtcagcg
      781 tctgtcactg aaggtggggc agtcggtggt acagctctgg cacaaggggc agtgtgtggc
      841 gcaacatccg cgcagcacac atgaatataa acacacaacc aatccgcttc acatgccaga
      901 acgccaccgg cggcatggga catggacacc ggaacgcctt atcgagcagg gaaaccgtac
      961 aggtccgtcc acaggccggg tggttgaatc catgctgaaa gccaaacccc atccggagct
     1021 ggcttaccgt gcagtcctgg gtctgctggc acttcagaaa aaatacggcc cggaacggct
     1081 ggaaaaggcc tgttacgttg ccctgcacta taacgccccg gacagacgct ttatcgacaa
     1141 tctgctgcgt catcatcggg ataacg
//
LOCUS       DAZRVY010000332         1162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZRVY010000332 DAZRVY010000000
VERSION     DAZRVY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.335"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHXOX_004542"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHXOX_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR7007807.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHXOX_004543"
     CDS             complement(510..>1162)
                     /locus_tag="ACHXOX_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR7007808.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZRVY010000333         1157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZRVY010000333 DAZRVY010000000
VERSION     DAZRVY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.336"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHXOX_004544"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHXOX_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHXOX_004545"
     CDS             572..>1157
                     /locus_tag="ACHXOX_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR7007809.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZRVY010000334         1148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZRVY010000334 DAZRVY010000000
VERSION     DAZRVY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.337"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHXOX_004546"
     CDS             <1..122
                     /locus_tag="ACHXOX_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007810.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHXOX_004547"
     CDS             119..376
                     /locus_tag="ACHXOX_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007811.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHXOX_004548"
     CDS             376..750
                     /locus_tag="ACHXOX_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7007812.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHXOX_004549"
     CDS             747..>1148
                     /locus_tag="ACHXOX_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7007813.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZRVY010000335         1148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZRVY010000335 DAZRVY010000000
VERSION     DAZRVY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.338"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHXOX_004550"
     CDS             <1..187
                     /locus_tag="ACHXOX_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR7007814.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHXOX_004551"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHXOX_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7007815.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          276 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgcttt
//
LOCUS       DAZRVY010000336         1141 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZRVY010000336 DAZRVY010000000
VERSION     DAZRVY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.339"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHXOX_004552"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHXOX_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..1109
                     /locus_tag="ACHXOX_004553"
                     /pseudo
     CDS             294..1109
                     /locus_tag="ACHXOX_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          325 a          234 c          272 g          310 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccctggg gactaatgcc agtcagttaa gcaactgact ggctcttttt cggggctgtg
     1141 g
//
LOCUS       DAZRVY010000337         1140 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZRVY010000337 DAZRVY010000000
VERSION     DAZRVY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.340"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHXOX_004554"
     CDS             <1..>1140
                     /locus_tag="ACHXOX_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR7007816.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZRVY010000338         1119 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZRVY010000338 DAZRVY010000000
VERSION     DAZRVY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.341"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(39..543)
                     /locus_tag="ACHXOX_004555"
                     /pseudo
     CDS             complement(39..543)
                     /locus_tag="ACHXOX_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
     gene            complement(560..>1119)
                     /locus_tag="ACHXOX_004556"
     CDS             complement(560..>1119)
                     /locus_tag="ACHXOX_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7007817.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          332 a          215 c          181 g          391 t
ORIGIN      
        1 agtgagttat taatatattt ttaaataaaa ttaagttatc attcatcgat gcattttata
       61 gaatcatcaa tggccataat agtactatta actctcaatt ttatattttt atcatcacta
      121 caaatattaa ctgaatttat atcaatgatt tttttattct tatcttttat ttactgtttc
      181 aatgcaatca gcggcaagtt ggagtggatt tagatttttc agatatggtc ttatgatctt
      241 tcccttactt aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct
      301 cattatatta gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag
      361 tccgagttca gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc
      421 ctctttagta ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc
      481 tctctgaaat tgtcttgcag taactgtact cgcagaaata gagaaaaaaa tacaattgtt
      541 aataactttg ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata
      601 ttaactcatt taatttttgc atgtcattag atgaaccagt tatacaaccc tcggaacaga
      661 atcgaggtcc taaagacgga ccgaaagcgt ttaaacaact ttgtgttaaa aatccatcac
      721 gattatatgt ttctgtattt gctgacggta ctaaacgtat accattaggt tttctattgg
      781 ttgctccagg accattaaca ttccagctcc ataccccttg aggtattgga cctctatttt
      841 gtaagtgtgt acaaccagga ttatttttac attgcatatt attaccatta ttaccagagg
      901 cgacaggaat agtaagcaca tcataagagt tatcatcatt ttgcgtatct gaaacgcatg
      961 aaagcatact taaagttatg ctgtataaac aggtcgctaa tcccaacggg tcaatatcag
     1021 ttatcggatt caacggatac tgataaaaat tccatccccc cttcagtcca atcttttcgc
     1081 gccggcgtgt acaccgggtc catctggttt tgcatctgc
//
LOCUS       DAZRVY010000339         1117 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZRVY010000339 DAZRVY010000000
VERSION     DAZRVY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.342"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHXOX_004557"
     CDS             84..1115
                     /locus_tag="ACHXOX_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR7007818.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZRVY010000340         1115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZRVY010000340 DAZRVY010000000
VERSION     DAZRVY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.343"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHXOX_004558"
     CDS             complement(<1..643)
                     /locus_tag="ACHXOX_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR7007819.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHXOX_004559"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHXOX_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR7007820.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          322 a          252 c          197 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAZRVY010000341         1088 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZRVY010000341 DAZRVY010000000
VERSION     DAZRVY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.344"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(270..992)
                     /locus_tag="ACHXOX_004560"
     CDS             complement(270..992)
                     /locus_tag="ACHXOX_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7007821.1"
                     /translation="MLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRARLLKQYQ
                     THLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLS
                     SDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANE
                     MYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRL
                     AEPFWQDHFLGARRILTEETIL"
BASE COUNT          281 a          272 c          269 g          266 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataa
//
LOCUS       DAZRVY010000342         1087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZRVY010000342 DAZRVY010000000
VERSION     DAZRVY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.345"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            528..>1087
                     /gene="fimB"
                     /locus_tag="ACHXOX_004561"
     CDS             528..>1087
                     /gene="fimB"
                     /locus_tag="ACHXOX_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR7007822.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          332 t
ORIGIN      
        1 ccaggataaa aaggaatgta acaatctcat ggcgtaagct gacgaaccag gcaggaataa
       61 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg tgttccatga
      121 aatagccatt ttgtggcaaa tggagttgac taataatgtc atatgtgaga cggctagttg
      181 aacgaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatatca
      241 catgatatat agataagatt agttgcatta atgatgaggg ttattattag attcgtatca
      301 ggttgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
      361 attataagta actatcgcta ctccatttaa gttcgctcag aagaactggt ccacttacgt
      421 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
      481 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
      541 ctgataacaa aaaaaggaac ttccttaccc atagtgaaat cgagtcactc cttaaagcag
      601 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      661 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      721 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
      781 aagttcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
      841 agtgggtatt tttatcacgt aaggggaatc cgctttctcg gcaacagttt taccatatta
      901 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      961 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     1021 ttgggcatcg caatattcgt catactgtct ggtataccac cagcaatgca gggcgttttt
     1081 acggcat
//
LOCUS       DAZRVY010000343         1086 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZRVY010000343 DAZRVY010000000
VERSION     DAZRVY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.346"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(506..>1086)
                     /locus_tag="ACHXOX_004562"
     CDS             complement(506..>1086)
                     /locus_tag="ACHXOX_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7007823.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          343 a          207 c          190 g          346 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacggt
//
LOCUS       DAZRVY010000344         1063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZRVY010000344 DAZRVY010000000
VERSION     DAZRVY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.347"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            9..944
                     /locus_tag="ACHXOX_004563"
                     /pseudo
     CDS             9..944
                     /locus_tag="ACHXOX_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            946..>1063
                     /locus_tag="ACHXOX_004564"
     CDS             946..>1063
                     /locus_tag="ACHXOX_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR7007824.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYC"
BASE COUNT          341 a          192 c          272 g          258 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      721 ctcacggttt ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg
      781 atagtctaaa tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa
      841 aagaatacag tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag
      901 ctaaagggaa tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa
      961 tgttgttatt tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat
     1021 aatatatttt taagtaattc ggattcagct gctttatatt gtg
//
LOCUS       DAZRVY010000345         1063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZRVY010000345 DAZRVY010000000
VERSION     DAZRVY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.348"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            289..>1063
                     /locus_tag="ACHXOX_004565"
     CDS             289..>1063
                     /locus_tag="ACHXOX_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007825.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNAKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYISDQYDLVQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETIS
                     STQLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGANFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLNINFTDANLGDTIFYIKNT"
BASE COUNT          410 a          202 c          154 g          297 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaatt taggtgacac tattttttac ataaagaaca ccc
//
LOCUS       DAZRVY010000346         1049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZRVY010000346 DAZRVY010000000
VERSION     DAZRVY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.349"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHXOX_004566"
     CDS             <1..120
                     /locus_tag="ACHXOX_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR7007826.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHXOX_004567"
     CDS             120..410
                     /locus_tag="ACHXOX_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR7007827.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHXOX_004568"
     CDS             407..961
                     /locus_tag="ACHXOX_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR7007828.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          299 a          201 c          298 g          251 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca tttgaaagag cccgcttta
//
LOCUS       DAZRVY010000347         1035 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZRVY010000347 DAZRVY010000000
VERSION     DAZRVY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.350"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            74..289
                     /gene="xisR"
                     /locus_tag="ACHXOX_004569"
     CDS             74..289
                     /gene="xisR"
                     /locus_tag="ACHXOX_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR7007829.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            291..>1035
                     /locus_tag="ACHXOX_004570"
     CDS             291..>1035
                     /locus_tag="ACHXOX_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR7007830.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          348 a          191 c          243 g          253 t
ORIGIN      
        1 catagctgat tattaataat caacatcggg cgtcaatttc agcctaacat tggcgcctgc
       61 cagaggtgat gcgatggcac aagtaatctt taatgaagag tggatggttg aatacggcct
      121 gatgcttcgc actggtctgg gtgccagaca aattgaagca taccgccaga actgttgggt
      181 ggaaggcttc cacttcaaac gagtatctcc tttagggaag ccagacagta agcgagggat
      241 tatctggtac aactatccga agataaatca gtttatcaaa gactcatgat atgtctaaat
      301 taccaacagg tgtcgagatt cgaggtaaat acattcgcat ctggttcatg tttcgaggaa
      361 aacgatgtcg ggaaacattg aaaggctggg agattactaa cagtaacatt aaaaaggccg
      421 ggaatttaag agcgttgata gttcatgaaa tcagttccgg tgaatttgag tatttaagac
      481 gttttcccca gtccagcact ggggcaaaaa tggtgacaac gagggtcata aaaacattcg
      541 gggagctttg tgatatctgg acaaaaatta aagagacaga gttaacaaca aacacaatga
      601 agaaaacgaa atcacaatta aaaacactca ggataataat ttgtgagagt accccgatat
      661 cgcatattcg ttatagcgat atcttaaact accggaatga actgctgcat ggagaaacgc
      721 tttacctaga taatccaaga tccaacaaaa aaggaagaac cgtgcgcaca gttgataact
      781 atatcgccct gctctgttcg ttgttacgtt ttgcgtatca gtcgggattt atatcaacca
      841 aaccatttga aggagtaaaa aaattacagc gaaacagaat aaagcctgac ccgttatcta
      901 aaacagaatt caatgcatta atggaaagtg aaaaaggaca gagccaaaac ttgtggaaat
      961 ttgccgttta ctccgggctt cgtcacgggg aactggcagc tctggcgtgg gaggatgtgg
     1021 atttcgagaa gggaa
//
LOCUS       DAZRVY010000348         1034 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZRVY010000348 DAZRVY010000000
VERSION     DAZRVY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.351"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..250
                     /locus_tag="ACHXOX_004571"
     CDS             <1..250
                     /locus_tag="ACHXOX_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007831.1"
                     /translation="WTTAKVNFTTSTYNIGKNTRNLSIGVHAYCSWTYLNGAPFGGFQ
                     QVYSDQNKVWYVNNYAWGNYESGGTITVTCLNLPGAGI"
     gene            complement(247..444)
                     /locus_tag="ACHXOX_004572"
     CDS             complement(247..444)
                     /locus_tag="ACHXOX_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein C'"
                     /protein_id="HFR7007832.1"
                     /translation="MNYSACKWYQSSVAMNHFIGGKSGGSIYYKPIQCPTGFIMTGTR
                     MYGIGDGVDEEHVDAYCCPFG"
     gene            complement(441..572)
                     /locus_tag="ACHXOX_004573"
     CDS             complement(441..572)
                     /locus_tag="ACHXOX_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007833.1"
                     /translation="MAMGSVVHLLFFLSEQCYLMPFSTYRIGTEASANPVGGLVAIK"
     gene            complement(760..>1034)
                     /locus_tag="ACHXOX_004574"
     CDS             complement(760..>1034)
                     /locus_tag="ACHXOX_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFR7007834.1"
                     /translation="KLAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
BASE COUNT          270 a          242 c          253 g          269 t
ORIGIN      
        1 ctggacaacg gcaaaagtga acttcactac cagtacgtac aatatcggga aaaacacccg
       61 aaatctgagc attggcgtac atgcgtactg ttcgtggaca taccttaatg gtgcgccttt
      121 tggtgggttt cagcaggtat actccgacca aaacaaggtt tggtatgtga ataattatgc
      181 gtggggaaat tatgagtccg gcggcacaat tactgttaca tgcctgaatc ttcctggtgc
      241 tgggatttaa ccgaaggggc aacaataagc atcaacgtgt tcctcatcaa cgccatcacc
      301 aatgccatac atgcgtgtac ctgtcatgat aaatccggtc gggcattgaa tgggtttgta
      361 gtagatagat cctccacttt tcccgccaat gaagtgattc attgctacag atgactgata
      421 ccacttacag gcggagtagt tcactttatt gccaccagac caccgaccgg attggcactg
      481 gcctccgtgc caatccggta cgtggagaag ggcatcaggt agcactgttc tgacaggaaa
      541 aatagcaaat ggacaacaga tcccattgcc atctggtttt tctgccagcc agtgtacctg
      601 gagtgtcagt aatgcagaaa atccgcacgg gtggaagccg aattattttg cggggtcagt
      661 tgcaacctat gatgccaatc gaattgtgaa atgcggcttc tatgacgaat acaactttta
      721 tggcggtact caccgaactg acctgtcggg gaaatgtagt tacatcgtgg tttgccagtg
      781 ataagtatcg tcactggcaa atggcgtaaa ctgtaattga accattttga tcttcggtcg
      841 tgtcgcaact ggcagtccat gcattgttgc ctgaaggcat ggaacgcgtg agccatataa
      901 atccggtatt tgatctgcat tgaccgcctc caccaatgac tttcttgcca gatggacatg
      961 ttgccgttgc tccaggccaa cgccacgcag tagctgaagc ggtaacaatt tggctatcac
     1021 ctgcgccaat tttt
//
LOCUS       DAZRVY010000349         1024 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZRVY010000349 DAZRVY010000000
VERSION     DAZRVY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.352"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            674..970
                     /locus_tag="ACHXOX_004575"
     CDS             674..970
                     /locus_tag="ACHXOX_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR7007835.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          303 a          198 c          220 g          303 t
ORIGIN      
        1 gtccttttaa gactggttat gaaggagcta tgaggttgat taggggttgg tacaagccca
       61 actaaccgaa tcaactatga agattttagg agggtaactg taaccatcac tttctaactt
      121 tcacgtagga aattgttaag tggtttgata gttacaccat gtgaccaagc atctatatat
      181 cgttatatcg ccctgtatat agcactatat gcagtgtcga gagaggaacg actaacacga
      241 tacagtttac cctgaaaaga gacttcacag attatttata tatttcaata agttagggta
      301 aaaagggact ataccgagac tgtatagaat ctgtgtagaa ttgcatagat acttggaaat
      361 ctactattaa attgcatagg gactcacctg atattgtccc gtttttaaca ggtctcctag
      421 aaaaccgaat agacttcatt caagggtcat tgaagtcata atcatttcag tagaaaaacc
      481 aagtctcgat tttaaatgca aatagcaatc atattcaata aggcaagacg atcaggatca
      541 gtgaaaatgt catttttggc gtaattgacc ttactttatg catggtctta acaaagacat
      601 catctttccg ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca
      661 aggagttgtg cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa
      721 acgcctttcc gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg
      781 tttgttgaca gctggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt
      841 tactggctca accgttattg ctgaatttga atcgctggaa gcagctcagg cctgggccga
      901 tgctgacccg tacgtagcgg caggtgtcta tgaacacgtt tcagtgaaac catttaagaa
      961 agtgttctga atagagattc aatatttaag ggcacggttt ttgcaaccgt gttcttgttt
     1021 ggcc
//
LOCUS       DAZRVY010000350         1023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZRVY010000350 DAZRVY010000000
VERSION     DAZRVY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.353"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHXOX_004576"
     CDS             complement(77..865)
                     /locus_tag="ACHXOX_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR7007836.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHXOX_004577"
     CDS             complement(905..>1023)
                     /locus_tag="ACHXOX_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR7007837.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZRVY010000351         1006 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZRVY010000351 DAZRVY010000000
VERSION     DAZRVY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.354"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            118..>1006
                     /locus_tag="ACHXOX_004578"
     CDS             118..>1006
                     /locus_tag="ACHXOX_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR7007838.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT          285 a          241 c          263 g          217 t
ORIGIN      
        1 gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta aaattcacga
       61 attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg agggcttatg
      121 tcactgtcaa ttgttcatac ccgcgcagcc ctgggagtaa atgcgccccc gatcactgtt
      181 gaggtacata tcagtaaagg tctacccggc ttaacgatgg tgggcttacc agaaacaact
      241 gtaaaagaag ctcgcgatcg cgtgcgcagc gccattatca atagcggata tgaatatccg
      301 gcgaaaaaaa tcaccatcaa ccttgctcca gccgatctgc caaaagaagg gggacgatat
      361 gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc caataagtta
      421 gatgaatatg aattagtcgg agaactggct cttacaggcg ctctgcgtgg cgttcccggt
      481 gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt cgcgaaagat
      541 aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga tcatctgcaa
      601 gctgtctgtg cgtttctgga aggtaagcac gctctcgaac gcccgaaacc aactgatgca
      661 gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggaaca aggaaagcga
      721 ggactggaaa ttaccgccgc tggcaggcac aaccttttac tgattgggcc gccgggaaca
      781 ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag caatgaagag
      841 gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca aaaacaatgg
      901 cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat ggtgggcggt
      961 ggtgcaattc cagggccagg tgaaatttcg ttggcgcata acggcg
//
LOCUS       DAZRVY010000352         1001 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZRVY010000352 DAZRVY010000000
VERSION     DAZRVY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.355"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>1001
                     /locus_tag="ACHXOX_004579"
     CDS             <1..>1001
                     /locus_tag="ACHXOX_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007839.1"
                     /translation="AMDCYAEHEGGNTVVIGYVPRISIPSNGKKGDKIWQSSEYFMNV
                     FCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSYIFGVTYNGVDYDSTAPNAIA
                     APECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPARVQLYIKLAKNANRVNKNLVL
                     PDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLDCFVNVALEPADGVVDFGKIN
                     SRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIF
                     HKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITY"
BASE COUNT          306 a          191 c          213 g          291 t
ORIGIN      
        1 gggcaatgga ctgctatgct gaacatgaag ggggaaatac cgtagtcatt ggttacgtac
       61 caagaatttc tatccccagt aatggtaaaa aaggtgataa gatctggcaa agcagtgagt
      121 attttatgaa tgtcttctgt aataatgcat tacctgcccc atcacctgga gaagaatacc
      181 catcagcctg gactaatata atgatgtttt taccaggcgg tcaggacttt tataatcaga
      241 actcctatat ttttggcgta acatacaatg gagttgatta tgattcgact gcccctaatg
      301 ctatcgcagc accagaatgc attgatataa aaggagcggg tacctttaat aatcactata
      361 aaaaccccgc tgtctgtagt ggaggtcctg agcctcaatt atcagtaacc tttccagctc
      421 gcgtacaact ctatattaaa ttagcaaaaa atgccaatag agttaataaa aacctggtgt
      481 tacctgatga atatatcgct ctagagttta aaggtatgag tggcgcagga gctatagaag
      541 tagacaaaaa tttgaccttc aggattcgtg gattaaataa tattcatgtc cttgactgct
      601 ttgttaatgt cgcactggaa ccagctgatg gcgttgttga ctttggtaaa ataaattccc
      661 gaacaattaa aaataccagc gttagtgaga cgtttagtgt agtcatgaca aaagatcctg
      721 gtgcggcctg tactgagcaa ttcaatattt tagggagctt tttcactacg gatattttga
      781 gtgattatag ccatctggat atgggtaatg gactgctatt gaagatattt cataaagatg
      841 gaacagcaac ggaatttaac cgcttctcac aatttgcttc tttttcatcg tctagtgcac
      901 cttcggtcac cgcaccattc agggcagaac ttagtgcgaa cccggcagag actgttgttg
      961 agggaccgtt tagtaaagac gtaatcctca aaatcaccta t
//
LOCUS       DAZRVY010000353          986 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZRVY010000353 DAZRVY010000000
VERSION     DAZRVY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.356"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            231..>986
                     /gene="gspA"
                     /locus_tag="ACHXOX_004580"
     CDS             231..>986
                     /gene="gspA"
                     /locus_tag="ACHXOX_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR7007840.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          281 a          199 c          237 g          269 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      481 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      541 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      601 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      661 ttaaaaaaca gggcattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      721 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      781 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      841 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      901 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      961 gtggaaccat tatcgctatt acctgt
//
LOCUS       DAZRVY010000354          974 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZRVY010000354 DAZRVY010000000
VERSION     DAZRVY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.357"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHXOX_004581"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHXOX_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR7007841.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZRVY010000355          969 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZRVY010000355 DAZRVY010000000
VERSION     DAZRVY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.358"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHXOX_004582"
     CDS             complement(<1..228)
                     /locus_tag="ACHXOX_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR7007842.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHXOX_004583"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHXOX_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>969)
                     /locus_tag="ACHXOX_004584"
     CDS             complement(586..>969)
                     /locus_tag="ACHXOX_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007843.1"
                     /translation="FKSSIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINLALS
                     FILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQL
                     NVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          317 a          165 c          188 g          299 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaa
//
LOCUS       DAZRVY010000356          961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZRVY010000356 DAZRVY010000000
VERSION     DAZRVY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.359"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHXOX_004585"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHXOX_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR7007844.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZRVY010000357          958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZRVY010000357 DAZRVY010000000
VERSION     DAZRVY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.360"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHXOX_004586"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHXOX_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZRVY010000358          946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZRVY010000358 DAZRVY010000000
VERSION     DAZRVY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.361"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..918)
                     /gene="rbn"
                     /locus_tag="ACHXOX_004587"
     CDS             complement(1..918)
                     /gene="rbn"
                     /locus_tag="ACHXOX_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR7007845.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT          234 a          240 c          236 g          236 t
ORIGIN      
        1 ttaaacgtta aacacggtga aatcattcgc cagttcagtc gccgggaaaa ttgacctgca
       61 ttcacgtaac aggtgttgac aacctttgtc atcatagcgc gagctgacgt gggtaatgat
      121 tagcttgccg actccagcct cacgggctag tgtcgcagcc tggcgtgtag agctatggcc
      181 gcgactattg gctttggctt ccatggttat atccagcgtc gcttcgtgca ccatgacatc
      241 gacaccttta gccaggtcaa gtgcggcatc gcaggggccg gtatcgccga aaatagcgag
      301 cgctttacct ggcactggag cagctaagta atctgcgccg ttaatctgcc ttccatcttc
      361 cagcgtgatt gttttgcccg cttttaattc ctgaaacagt gggccaggcg gcacgccagc
      421 agcttttaat gcctgggcat ttaatgcacc cggtttatca tgttcttcaa tacgatagcc
      481 ataacattcc agtgggtgtt ccatcggata agcggtcact ttgcgcaggc catcatcgag
      541 aatttcgcca gcgccaattt cgacaatttc cagcggataa tcggtccatg agccgctaat
      601 ccgcagcgcg gtttccacaa attcacggat accttgcgga ccataaatcg ttaagggttg
      661 gataatgcct gacatagaac gactgcacag caagccgggt aaaccaaaga gatgatcgcc
      721 atgaaggtga ctgataaaaa tcttgtccag tttcccaggg ttaaaggcgg tatgcagtag
      781 ctgatgctgg gtgccttcac cgcagtcaaa caaccaaagt ccgctctggg tcggatgttg
      841 caggttaagc aatattgccg tgacattgcg cgtgcgggtt ggcacaccgg ctgaagtacc
      901 taaaaaaatt aattccatga gttcatcacg tttcaaaaaa tattcc
//
LOCUS       DAZRVY010000359          940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZRVY010000359 DAZRVY010000000
VERSION     DAZRVY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.362"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHXOX_004588"
     CDS             <1..164
                     /locus_tag="ACHXOX_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR7007846.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>940
                     /locus_tag="ACHXOX_004589"
     CDS             170..>940
                     /locus_tag="ACHXOX_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7007847.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRD"
BASE COUNT          277 a          215 c          231 g          217 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat
//
LOCUS       DAZRVY010000360          934 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZRVY010000360 DAZRVY010000000
VERSION     DAZRVY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.363"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(196..>934)
                     /locus_tag="ACHXOX_004590"
     CDS             complement(196..>934)
                     /locus_tag="ACHXOX_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7007848.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAZRVY010000361          921 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZRVY010000361 DAZRVY010000000
VERSION     DAZRVY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.364"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHXOX_004591"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="ACHXOX_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR7007849.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          267 a          146 c          229 g          279 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg a
//
LOCUS       DAZRVY010000362          907 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZRVY010000362 DAZRVY010000000
VERSION     DAZRVY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.365"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..796
                     /locus_tag="ACHXOX_004592"
     CDS             <1..796
                     /locus_tag="ACHXOX_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7007850.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(839..>907)
                     /locus_tag="ACHXOX_004593"
     CDS             complement(839..>907)
                     /locus_tag="ACHXOX_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007851.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          259 a          184 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccgcc
//
LOCUS       DAZRVY010000363          901 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZRVY010000363 DAZRVY010000000
VERSION     DAZRVY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.366"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>901
                     /locus_tag="ACHXOX_004594"
     rRNA            <1..>901
                     /locus_tag="ACHXOX_004594"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          247 a          194 c          286 g          174 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 g
//
LOCUS       DAZRVY010000364          899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZRVY010000364 DAZRVY010000000
VERSION     DAZRVY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.367"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>899)
                     /locus_tag="ACHXOX_004595"
     CDS             complement(<1..>899)
                     /locus_tag="ACHXOX_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR7007852.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKIL"
BASE COUNT          300 a          158 c          111 g          330 t
ORIGIN      
        1 aggattttca tcattcttgt tctaattttt aattcattac ccatcttttc ttcaaaaatc
       61 ttaacgacat aacccggaag tttatttaca atttcttttg ttttttcttc atcaccataa
      121 acatatccta actcattaag atacagctgt tcgccctcat aacacttaag taattgatag
      181 aagtaccact tagcaaaatc aggttgcttc atgaaaaaat ccttcaaaaa aatatcattt
      241 actaaaatgc tgacaggcac ttcattaaat agtaacttaa catcatcaaa taaccttagt
      301 attaagcgtg atttttcttg attgcttaag ctaaaatcat tgacatatgc aaccaactta
      361 tcccaaatca attcctgtaa tttacggcta tttttatagg gttctttatc gagattgaaa
      421 aataaggtct cagctatttt aattttagat tgatcgcagc taaaatcttt agattgatcg
      481 cagctaaaat caatatgatt tatcacggag catgcatact ttatcttttc agaatcacca
      541 aaatattgca ttcttttatt aaaacaccaa tccagtattc ttacatcatt aatatatggg
      601 gattttaaaa aagtctccag aatcgaatcg atgaccggtg taaggttaac ttttgcatca
      661 tctaaggaat caataagaat atgagacagg ttaactttga tgtcattaat ttcattgtca
      721 gtagctttct tattattaag aagataatta tttactgcgt taaggtcatc tatttctttc
      781 agtatccctt tttcaccaag caagtatgaa atcgattcat tacatacata aggcattaat
      841 agcagatgtt ctttccttat tgctaataat gcattgttca atggtttttc taataaatc
//
LOCUS       DAZRVY010000365          895 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZRVY010000365 DAZRVY010000000
VERSION     DAZRVY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.368"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>895
                     /locus_tag="ACHXOX_004596"
     CDS             <1..>895
                     /locus_tag="ACHXOX_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR7007853.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLH"
BASE COUNT          321 a          179 c          165 g          230 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaa
//
LOCUS       DAZRVY010000366          883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZRVY010000366 DAZRVY010000000
VERSION     DAZRVY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.369"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            84..464
                     /locus_tag="ACHXOX_004597"
     CDS             84..464
                     /locus_tag="ACHXOX_004597"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7007854.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISSEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            461..808
                     /gene="tnpB"
                     /locus_tag="ACHXOX_004598"
     CDS             461..808
                     /gene="tnpB"
                     /locus_tag="ACHXOX_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR7007855.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          221 a          240 c          228 g          194 t
ORIGIN      
        1 cagcctgaac cgtctggtca gaatctgacg aattagacaa agtggtgtcc accaaataag
       61 tagtgggaac caaagtgtca gatatgcaga aaaatgtgac tcccggcagg cgaaaaggct
      121 gccctaatta tcctcccgaa tttaaacagc agctcgttgc tgcctcctgt gaacccggga
      181 tatccatctc aaaacttgct cttgaaaatg gcattaacgc caatctgttg ttcaaatggc
      241 gacaacaatg gcgcgaggga aagctgctat taccttcttc agagagcccc cagctacttc
      301 ctgtgactct cgatgcagct gccgaacagc cagaatcgct cgcagaggac ccgaaaaccc
      361 tcagtatcag cagtgaggta acgttccggc acgggacgct ccgcttcaat ggcaatgtca
      421 gcgaaaagct cctgactctg ctgatacagg aactgaagcg atgatcccgt taccttccgg
      481 gatcaaaatt tggctggttg ccggtatcac cgatatgaga aatggcttca acggcctggc
      541 tgcgaaagta cagacggcgc tgaaagacga tcccatgtcc ggccatgttt tcattttccg
      601 gggccgcagc ggcagtcagg ttaaactgct gtggtccacc agtgacggac tgtgcctcct
      661 gaccaaacgg ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt
      721 ccttacacag gcgcagctgg cgatgctgct ggaaggtatc gactggcgac agcctaagcg
      781 gctgctgacc tccctgacca tgctgtaaat ctctttatcc tggttgtcac agaataagcc
      841 cggtaaaata cgggcttatg aacgacatct cttctgacga cat
//
LOCUS       DAZRVY010000367          875 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZRVY010000367 DAZRVY010000000
VERSION     DAZRVY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.370"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>875
                     /gene="cadA"
                     /locus_tag="ACHXOX_004599"
     CDS             <1..>875
                     /gene="cadA"
                     /locus_tag="ACHXOX_004599"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR7007856.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKEL"
BASE COUNT          237 a          220 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgc
//
LOCUS       DAZRVY010000368          861 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZRVY010000368 DAZRVY010000000
VERSION     DAZRVY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.371"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHXOX_004600"
     CDS             <1..547
                     /locus_tag="ACHXOX_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007857.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>861
                     /locus_tag="ACHXOX_004601"
     CDS             623..>861
                     /locus_tag="ACHXOX_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007858.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFR"
BASE COUNT          210 a          189 c          280 g          182 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gaacccggtg ctgggtgcga aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc
      841 gctgccgttt attcttttcc g
//
LOCUS       DAZRVY010000369          832 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZRVY010000369 DAZRVY010000000
VERSION     DAZRVY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.372"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHXOX_004602"
     CDS             complement(<1..431)
                     /locus_tag="ACHXOX_004602"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7007859.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..757
                     /locus_tag="ACHXOX_004603"
     CDS             488..757
                     /locus_tag="ACHXOX_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7007860.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT          243 a          165 c          196 g          228 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tggtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat tcagtgagca
      601 tattgcgcca cttcaggatg ctgtagatct ggagatcgca acggaggaag aaaactcgtt
      661 gctggaagcc tggaagaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc
      721 tgatattgag tggccggaag aaccagacac aatgtaaggc agtatgactt tccgtgagac
      781 agatgctggc caacgtggga tatttaacgc gggttaacat cgttgtcaga gc
//
LOCUS       DAZRVY010000370          825 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZRVY010000370 DAZRVY010000000
VERSION     DAZRVY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.373"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            401..805
                     /gene="stpA"
                     /locus_tag="ACHXOX_004604"
     CDS             401..805
                     /gene="stpA"
                     /locus_tag="ACHXOX_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR7007861.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          242 a          175 c          194 g          214 t
ORIGIN      
        1 gcgccccacc agacccggtg acatcacccc ccggatcatg gacgactggg ggcagtaatc
       61 atgccgggtc tggctggcta acaccccatt ataaggggta cgaataaagt aacgggggtt
      121 cttttattct gttatattgc atttttattc acttcggttt tgtcaggctt gcggaattag
      181 cgagcagaga gcgccgttct gttcaccaat aatctaaatt actcatcaaa atggctaata
      241 tataatcttg aaattattct gagagtttca gaaaataaca gctgaaataa tctcgcgcaa
      301 gactgtaaat agattaaatt ttgtggaaat ataataagtg atcgcttaca ctacgcgacg
      361 aaatactttt ttgttttggc gttaaaaggt tttctttatt atgtccgtaa tgttacaaag
      421 tttaaataac attcgcaccc tccgtgcgat ggctcgcgaa ttctccattg acgttcttga
      481 agaaatgctc gaaaaattca gggttgtcac taaagaaaga cgtgaagaag aagaacagca
      541 gcagcgtgaa ctggctgagc gtcaggaaaa aattagcacc tggctggagc tgatgaaggc
      601 tgacggaatt aacccggaag agttattggg taatagctct gctgctgcac cacgcgctgg
      661 taaaaaacgc cagccgcgtc cggcgaaata taaattcacc gatgttaacg gtgaaactaa
      721 aacctggacc ggtcagggcc gtacaccgaa gccaattgct caggcgctgg cagaaggtaa
      781 atctctcgac gatttcctga tctaattact gaggccggat gtcgc
//
LOCUS       DAZRVY010000371          815 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZRVY010000371 DAZRVY010000000
VERSION     DAZRVY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.374"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHXOX_004605"
     CDS             complement(246..>815)
                     /locus_tag="ACHXOX_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7007862.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZRVY010000372          803 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZRVY010000372 DAZRVY010000000
VERSION     DAZRVY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.375"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHXOX_004606"
     CDS             complement(<1..494)
                     /locus_tag="ACHXOX_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007863.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          265 a          169 c          145 g          224 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
      661 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aataagatat
      721 gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
      781 tgtaattagc ccagaacttt agc
//
LOCUS       DAZRVY010000373          799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZRVY010000373 DAZRVY010000000
VERSION     DAZRVY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.376"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHXOX_004607"
     CDS             complement(<1..741)
                     /locus_tag="ACHXOX_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR7007864.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZRVY010000374          790 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZRVY010000374 DAZRVY010000000
VERSION     DAZRVY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.377"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHXOX_004608"
     CDS             complement(<1..458)
                     /locus_tag="ACHXOX_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR7007865.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHXOX_004609"
     CDS             complement(530..742)
                     /locus_tag="ACHXOX_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007866.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          204 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagaca
//
LOCUS       DAZRVY010000375          775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZRVY010000375 DAZRVY010000000
VERSION     DAZRVY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.378"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHXOX_004610"
     CDS             <1..343
                     /locus_tag="ACHXOX_004610"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR7007867.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="ACHXOX_004611"
     CDS             complement(405..>775)
                     /locus_tag="ACHXOX_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR7007868.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAZRVY010000376          765 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZRVY010000376 DAZRVY010000000
VERSION     DAZRVY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.379"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(81..>765)
                     /locus_tag="ACHXOX_004612"
     CDS             complement(81..>765)
                     /locus_tag="ACHXOX_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7007869.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQLR"
BASE COUNT          176 a          193 c          189 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatggt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAZRVY010000377          756 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZRVY010000377 DAZRVY010000000
VERSION     DAZRVY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.380"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            194..756
                     /locus_tag="ACHXOX_004613"
                     /pseudo
     CDS             194..756
                     /locus_tag="ACHXOX_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          257 a          144 c          133 g          222 t
ORIGIN      
        1 gtgatgatta ttaatacact gcagagaaat aaaaaacaat ctgaagcatg ctctgaaaca
       61 tcctactctg tcactctaat gatggcacct tgctcttctt ataaagaagg gaacaattaa
      121 gaatattaaa ttttcaaact ttcatcaact tcacatcaag aaattaaaaa atttaaacat
      181 taatctaaat aataacaacc tgatcacata atcgggtacg attttatccg ataactataa
      241 tgctataact ctgggaaatg gttggaatac accgctgggt gccatctctt tttacgctgc
      301 ccgatcgagt agtaagttaa ataatgatac cagacacgaa ggtacgagct atcaggtcgc
      361 ctataataaa tatctagtac aaacagccac tcgatttagc gtggccgcct ggcgttatgc
      421 ttcgcaggat tacaggacat tcagcgacca tctttacgaa aatgataaac actatcatca
      481 gagtgattat gatgattttt atgatattgg cagaaaaaat agcctttctg ccaatattat
      541 gcaaccttta tccaataatc tgggaaatgt atcattaagt gcgctttggc ggaattactg
      601 ggggcgaagt ggaaatgcta aagattacca attcagttac tccaataact ggcaacacat
      661 tagttatact ttctctgcca gccaatctta tgatgaaaat aataaagaag aggagcgttt
      721 taatctgttt atctccattc ctttctactg ggggga
//
LOCUS       DAZRVY010000378          752 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZRVY010000378 DAZRVY010000000
VERSION     DAZRVY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.381"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          237 a          149 c          144 g          222 t
ORIGIN      
        1 gccctctcag acgctctctg acgcaaaaaa ctcaaaaaga caagaaaaaa cagggtaaac
       61 ataaaaaatg cgtcagaatc gtttttagcg cgttgcataa ctatgcacga aagcgacccg
      121 cgatcggttt tgtgtgttct gaataccgaa cgggtcaaaa tgacacgttt tctctccctc
      181 aatggagcgt tctatacaga gatttttgtt cccctatcca ttattctgat taccgggttt
      241 ccaagctgaa aacaggactt ttctgcgacc agtttttgcg aatatagtca cataaaccac
      301 cgattgagtg acagttactg aaacaactat ttcgacacaa aatctcgtct attcaacatc
      361 atgcaaattt gtcagtgact gaatttagtg acagaaccct ctggaattct gccgtgtaac
      421 cacttatatg cagacctgcc ccgttgctct gggctttcag gctgcgatga gtgatgagcg
      481 atgatttcca tacagatgat atatcgactg gaattgattg cctgcaatta gatttagttt
      541 tattgtgtgg atcgttttag ttagtgggga tgaatttccg ataaattaac caatggaaag
      601 gagatgatat gaataaaaag ataactggat tattaataat tagtctggcg tcaattttct
      661 taattgattg cacccagtgc cattaacatc tatctacaca aaaatagagg aaaacaattt
      721 tgatatatca aataattaca agcagctcca tt
//
LOCUS       DAZRVY010000379          751 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZRVY010000379 DAZRVY010000000
VERSION     DAZRVY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.382"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..694)
                     /locus_tag="ACHXOX_004614"
     CDS             complement(<1..694)
                     /locus_tag="ACHXOX_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7007870.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRA"
BASE COUNT          177 a          209 c          178 g          187 t
ORIGIN      
        1 ccgcgcgcca cgccatagca atgccatcgc cagaagaaat atccggattg gtggtgtact
       61 gatacacctt cgacgcaccg ccggttgcca gtaccactgc ttttgcgtgg caggtttcca
      121 ccgtttcttt atttcggttc catacccacg cgcctacaac ccgtcgcgtg cccggcaggc
      181 caattttgtc agagacaatc agatcaactg cgttgctgcg ctccagcaca cgaatattcg
      241 gatggttctg cgccttgctc accagcgtgg attgtacttc tctaccggtg gcgtcggcgg
      301 catgaagaat acgacggtga ctatgtccac cttcacgggt cagatggtag ctttcttcgc
      361 cattcggttg aacgtgggta tcaaacaaaa ccccctggtc aattaaccat tgcacacagg
      421 aacgagcatt gctggcgaca aattcaactg catggcgatc gcaaatacca gccccggcaa
      481 tcaatgtgtc ttccacatgc gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac
      541 cgccctgggc ataaaatgtt gaaccttccg ttaccgggcc tttacttaga acgatgacct
      601 gatgctggtc agccaggcgt agcgccagtg aaagtccggc tgcgccgcta ccaataatca
      661 acacgtcaca tgaatgttca gggagagtat tcattttctt tgtttaattt actaaacatt
      721 gtttggtcag catagcatca tgttgtgcga a
//
LOCUS       DAZRVY010000380          746 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZRVY010000380 DAZRVY010000000
VERSION     DAZRVY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.383"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHXOX_004615"
     CDS             <1..400
                     /locus_tag="ACHXOX_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR7007871.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          261 a          138 c          141 g          206 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgt
//
LOCUS       DAZRVY010000381          743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZRVY010000381 DAZRVY010000000
VERSION     DAZRVY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.384"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHXOX_004616"
     CDS             complement(<1..396)
                     /locus_tag="ACHXOX_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7007872.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          173 c          108 g          229 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZRVY010000382          736 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZRVY010000382 DAZRVY010000000
VERSION     DAZRVY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.385"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHXOX_004617"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHXOX_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZRVY010000383          724 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZRVY010000383 DAZRVY010000000
VERSION     DAZRVY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.386"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          203 a          141 c          147 g          233 t
ORIGIN      
        1 tataaaatct cttctgatgt ttaattgatt tgaatgttcg taagctatat cacttactca
       61 atccatttta cccagagtca taaaatagag tgtctgttga tctcattttc atattccttt
      121 cagactgaag tgcgttttca tcacccgatg agaaagaact gaagtgatgt ttgccataga
      181 ttgcgagatg ccctgtctca ttgactatcg cggtgcacct ttatacctgt tataccagat
      241 caaaaatcac gcaagccata caacaaaacg agatttgcaa ttcgtgtcac aaaatatgtc
      301 gatctttttc taagaggaag atgccatgtg aagccagacg aacacttgag gtggtcttca
      361 aaaactaaag gatttctttt aactgtttgt tttataaaaa atttatttag ggtctaaaca
      421 agggaagctt tgcaagctaa ctcagtgagc gtggtgaaaa caagtgttta cccgccatca
      481 ggctgatcat aattctcacc atgaaatatg tttcctgatt tgtggcttgt aactggtcgc
      541 ttctgaagtc gatctagaga ggcttgttga tgttggtgtt ttcaggatga tgtttcactt
      601 agtttgtttg ccgtatcgcc cggcgaatag ctgtgattga ggaaggttta agtcgcagtg
      661 accaaagcca tatttaccca cgaatgtaga tgaaaaagcg tctcctgcgt tcccccttat
      721 ctcc
//
LOCUS       DAZRVY010000384          717 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZRVY010000384 DAZRVY010000000
VERSION     DAZRVY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.387"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..375
                     /locus_tag="ACHXOX_004618"
                     /pseudo
     CDS             1..375
                     /locus_tag="ACHXOX_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          264 a          135 c          121 g          197 t
ORIGIN      
        1 aattaacaaa agaaatatat aaaagctcat cgaaaatcaa caattgcttg aacagaatta
       61 aattaccaga gatgaaagaa ttctcatcag aaaaaaatat atgattacat cgatataatc
      121 attgaagatt atgaaaatat caaagaaaat gcttatctgg tcgatccaaa aattaattac
      181 actatagatt taaacataga agactctagc tcagaggaat tcctttttga taatagcctc
      241 gagaaagacg aaaattcttc ggacaattgc tttgaggtcg aaaaatataa cgcatatgaa
      301 gcatataact cagagaatct atattttacc agagaggaat atacgtatga ttacgacctt
      361 ttaaatgcaa tatagacata ataacttctt acaaaatcta taattaatac cgtagatatt
      421 cattcatctt accctgtgca ccaaaagcaa aaactcactg acgcgggttt gaaactgatg
      481 gcaaccgcaa aaatgcctga tgcgctacgc ttatcaggcc tacgccatct ctacaatata
      541 ttgaatttgc gtgcttttgt aggcaggata aggcgttcac gcagcatccg gcatgaataa
      601 agcgcactca acaatctgaa acccgccgga acggtttatt acgtacatca ggtgaaactg
      661 accgataagc cgctttcttt tgggtatagt gtcgtggaca gtcattcatc tttctgc
//
LOCUS       DAZRVY010000385          714 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZRVY010000385 DAZRVY010000000
VERSION     DAZRVY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.388"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..714
                     /locus_tag="ACHXOX_004619"
                     /pseudo
     CDS             1..714
                     /locus_tag="ACHXOX_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          137 a          241 c          193 g          143 t
ORIGIN      
        1 gctacgcacg ctgaaactgg ctgacgagca aatcgccgaa ctctccatgc tctgcggctt
       61 cgatgatgat cttgccgcac agacaacgca ggccagcaac cgtatccgcg gccttctgac
      121 ccagatacat ccggcactgg agcgcgttct cggtccgaga cttgagcacc cggcggtact
      181 cgatcttctc cagcgatatc cctcaccaga aaaactcgct tcgctgggct tcgctgggtg
      241 agaagaagct ggcagcccag ctctgcaaac ttgcgcctcg tctgggtaaa cgccttgcag
      301 cagacatagc tcaggcactg gccgaacaaa ccgtcgtcgt tcccggcacg aatgccgctg
      361 ccgtagtact gccacgtctg gcactccagc tcatcacgct gcgtaagcaa agagacgagg
      421 tggcgcttga ggtagaacag cgagttcttg ctcaccctct ttacccggtc ctgaccagta
      481 tgcccggagt cggtgtcagg accgcagcca gactcctcac cgaggtcgcc tgccgcgcct
      541 tcgcctctgc cgcacatctc gctgcttatg ctggccttgc gccggtaact cggcgatccg
      601 gctcgtcaat acgcggtgag catccctcgc gacggggtaa taaagctctc aaacgggcgt
      661 tgttcctgtc ggccttcgcc gcgctcaggg atccgctctc cagggcttac taca
//
LOCUS       DAZRVY010000386          704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZRVY010000386 DAZRVY010000000
VERSION     DAZRVY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.389"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          217 a          172 c          135 g          180 t
ORIGIN      
        1 gttgatattt tttccatctg atacatatcc cgccccccga cagacctgtg ctatcggaaa
       61 gaaaaaaggc catcagcaaa actctgatgg cctgaatcac ctttaccaat attgtatgaa
      121 aaaacacgca cgactaattg acaataattt tcatttccat taaaaaagat cgcgtagcat
      181 tcttaattca tgaggaactt tacccgccag caatctgagt agcgaaagct gttcatcccc
      241 aatgtttttg ctggcgggtc cttttttcta cggtcctcct ccccggaggg gggcatttca
      301 ttatttttct aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa
      361 ataacagcat gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcgct
      421 gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc ccggggtttg cttagaatat
      481 tttgtaagtt gtccgaagag atatttacaa caccagaatg atgaatcatc agtcccctgc
      541 cagaggttca ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac
      601 tggaactgca acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac
      661 acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa tctc
//
LOCUS       DAZRVY010000387          701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZRVY010000387 DAZRVY010000000
VERSION     DAZRVY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.390"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            132..>701
                     /locus_tag="ACHXOX_004620"
     CDS             132..>701
                     /locus_tag="ACHXOX_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR7007873.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELD"
BASE COUNT          196 a          159 c          196 g          150 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga c
//
LOCUS       DAZRVY010000388          698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZRVY010000388 DAZRVY010000000
VERSION     DAZRVY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.391"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHXOX_004621"
     CDS             <1..>698
                     /locus_tag="ACHXOX_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007874.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZRVY010000389          698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZRVY010000389 DAZRVY010000000
VERSION     DAZRVY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.392"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            19..>698
                     /locus_tag="ACHXOX_004622"
     CDS             19..>698
                     /locus_tag="ACHXOX_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7007875.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          218 a          150 c          126 g          204 t
ORIGIN      
        1 tccttctgcg aggttattat gcttcctgta aataatcccc ccctatccac tggaaacgtc
       61 tctttttaca gaactacatc aatcgacaat gttcacaata attatctctc cgaatgggtt
      121 gaatggacta aaaacagcat ttccggagaa aacagggaaa ctgcttttac ccggctccaa
      181 ttatgtctgg agaacagtga aacatcgttg gacttatctt gtttaggtct cagatctcta
      241 ccacgattgc ctgacaatct tgatgaaatt aatgtaagca ataaccaact atcaatgctc
      301 cccgagctac caagggcatt gaaagagctg aatgcaagca gtaatcaatt atctgcactt
      361 cctgaattac cagtgtcgct ggaatatata aatgtgagtg ataaccattt gttcgcactt
      421 cctgaattac ctgcgtcact agaatatatt aatgtaagtg acaatcacct gtctgtactt
      481 ccgaggttac caatgtcatt ggaattactt gatgcagcca gaaatgcttt ggaagtaata
      541 ccagattttc cagaaagaga tgatcatatt ataagaatat tctggcttaa tcagaaccgg
      601 atcacggcaa ttccggaaag catacttggc ctcagttctg atagcgttgt caatcttaga
      661 gaaaatcaac tatctcccag aataatgcaa actttgtt
//
LOCUS       DAZRVY010000390          695 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZRVY010000390 DAZRVY010000000
VERSION     DAZRVY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.393"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHXOX_004623"
     CDS             <1..>695
                     /locus_tag="ACHXOX_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7007876.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZRVY010000391          690 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZRVY010000391 DAZRVY010000000
VERSION     DAZRVY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.394"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZRVY010000392          684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZRVY010000392 DAZRVY010000000
VERSION     DAZRVY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.395"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHXOX_004624"
     CDS             <1..560
                     /locus_tag="ACHXOX_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR7007877.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHXOX_004625"
     CDS             complement(616..>684)
                     /locus_tag="ACHXOX_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007878.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZRVY010000393          672 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZRVY010000393 DAZRVY010000000
VERSION     DAZRVY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.396"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            126..>672
                     /locus_tag="ACHXOX_004626"
     CDS             126..>672
                     /locus_tag="ACHXOX_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7007879.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          253 a          135 c           90 g          194 t
ORIGIN      
        1 aactgacaaa aattaatatt tctgatagtt ataaactgaa atttcttaat cttgattata
       61 acaagctagc atcgctggat gtctcccggc aagaatctct gattgagtta agtgcccatc
      121 acaatatgat cactgacctt atattacaca atcatcccag gatgaaaaca atcactttaa
      181 acgacaacca tattgcacat ttaaacgcca aaaacactac aaaactggaa tatttaaact
      241 taagcaataa caatttactg ccaaccaatg acattgatca actaatatca tcaaaacatc
      301 tttggcatgt attagttaac ggcatcaaca atgatccact tgcacaaatg cagtactgga
      361 ctgcagtaag aaatataatt gatgacacta atgaagtgac cattgattta tcaggactta
      421 atttaaccac tcaaccacca gggctgcaaa acttcacatc tatcaatctt gataataacc
      481 aactcacaca ttttgatgca accaactacg atagactcgt aaagctaagt ctgaatagta
      541 atgctcttga gtcaataaat tttcctcaag gcagaaatgt aagtattaca catatatcta
      601 tgaataataa tgctctcaga aatattgata tagataggct ttcatcagtt acttatttta
      661 gtgcggcaca ta
//
LOCUS       DAZRVY010000394          661 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZRVY010000394 DAZRVY010000000
VERSION     DAZRVY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.397"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>661
                     /locus_tag="ACHXOX_004627"
     CDS             <1..>661
                     /locus_tag="ACHXOX_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7007880.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          144 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 t
//
LOCUS       DAZRVY010000395          657 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZRVY010000395 DAZRVY010000000
VERSION     DAZRVY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.398"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>657)
                     /locus_tag="ACHXOX_004628"
     CDS             complement(<1..>657)
                     /locus_tag="ACHXOX_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7007881.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     S"
BASE COUNT          105 a          248 c          202 g          102 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt ccatcacccc cgtcaccgcg cccgccacgt caccctccgc
      421 tgcccggtga aaggccagcg tccgcccgaa gccgtccacc acgcccgtca gcacccggta
      481 tgcgggcggc tccggcggca gcacctcgtc cgcccccggc acccgctccg gccagctgag
      541 tatccaccac ggcccctgaa ggctgtttgt cgccaggtac acatgcggac tcaggcgaac
      601 atcttccggc agcacctgcc agagcgcgct tagcggctgc gaactgtgct gcgccgc
//
LOCUS       DAZRVY010000396          653 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZRVY010000396 DAZRVY010000000
VERSION     DAZRVY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.399"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..651
                     /locus_tag="ACHXOX_004629"
     CDS             <1..651
                     /locus_tag="ACHXOX_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7007882.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          222 a          110 c          165 g          156 t
ORIGIN      
        1 gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga tattgcagag
       61 aagatacaaa aacagggagg tgattattta ttcgcggtaa aaggaaacca ggggcggcta
      121 aataaagcct ttgagaaaaa atttccgctg acagaattaa ataatccagc gcatgacagt
      181 tccgcaatga gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat
      241 gtccctgatg aacttattga tttcacgttt gaatggaaag ggctgaaaaa attatgcgtg
      301 gcagtctcct ttcgctcaat aatagcagag caaaagaaag agcccgaaat gatggtcaga
      361 tattatatca gttctgctga tttaaccgct gagaagtttg cgacagcgat tcgaaatcac
      421 tggcacgtgg agaataatct gcactggcgt ctggacgtgg taatgaatga agacgactgc
      481 aaaataagaa gaggaaatgc agcagaatta ttttcaggga tacggcacat tgctattaat
      541 gttttgacga atgataaggt attcaaggca gggttaagac gtaagatgcg aaaagcagcc
      601 atggacagaa actatctcgc gtcagtcctt gcggggagcg gactttcgta atc
//
LOCUS       DAZRVY010000397          650 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZRVY010000397 DAZRVY010000000
VERSION     DAZRVY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.400"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHXOX_004630"
     CDS             <1..194
                     /locus_tag="ACHXOX_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR7007883.1"
                     /translation="VDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNL
                     HYIDTGAVFGGELTLVQLQ"
     gene            complement(195..386)
                     /locus_tag="ACHXOX_004631"
     CDS             complement(195..386)
                     /locus_tag="ACHXOX_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR7007884.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(491..>650)
                     /locus_tag="ACHXOX_004632"
     CDS             complement(491..>650)
                     /locus_tag="ACHXOX_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR7007885.1"
                     /translation="LTIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQS
                     DAWIMRLA"
BASE COUNT          184 a          148 c          160 g          158 t
ORIGIN      
        1 acgttgattt gcatcaggtc ttgtggagcc gctcgcgatt aggtgaacgc caaaaagggc
       61 agggaattac aggtgctgat catttctggt ttggtcatac accgttgcga catcgcgtgg
      121 atattggcaa cctgcattat attgataccg gtgctgtctt tgggggcgaa ctgactcttg
      181 tgcaattgca ataattaaaa atcaccgtac tcctgtgcag gtcgccagaa accatctata
      241 aaatcctcaa tcggaaaaca accgccatgg cggatccgtt gatcgctcat agaataaaga
      301 cactgctgtt ccgtgttgta gacatccaca acaatatctt cacaaccgcc atccaggtag
      361 caaacaaaaa gtaccagcgc gaacatttca tccccgaagt gtggtgccgt accgttaagt
      421 ttaggagaga ttttacaacg ggggaataac caggacaaat aacccgccat cccgacgggt
      481 tctttttgga tcaggcaaga cgcataatcc aggcatcaga ttgttgatcg taacgggtac
      541 ggtaaaggac agagtgttcg ccatttagca gggcagggat actggtttca gcatcccagg
      601 catcgagttg catacataaa tcggggtcag atttacaagg aatggttaac
//
LOCUS       DAZRVY010000398          649 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZRVY010000398 DAZRVY010000000
VERSION     DAZRVY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.401"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHXOX_004633"
     CDS             <1..216
                     /locus_tag="ACHXOX_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR7007886.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHXOX_004634"
     CDS             complement(161..298)
                     /locus_tag="ACHXOX_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR7007887.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHXOX_004635"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHXOX_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHXOX_004636"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHXOX_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          185 a          164 c          129 g          171 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgaccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgc
//
LOCUS       DAZRVY010000399          648 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZRVY010000399 DAZRVY010000000
VERSION     DAZRVY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.402"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHXOX_004637"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHXOX_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHXOX_004638"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHXOX_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          174 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaa
//
LOCUS       DAZRVY010000400          641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZRVY010000400 DAZRVY010000000
VERSION     DAZRVY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.403"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHXOX_004639"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHXOX_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR7007888.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZRVY010000401          637 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZRVY010000401 DAZRVY010000000
VERSION     DAZRVY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.404"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>637
                     /locus_tag="ACHXOX_004640"
     rRNA            <1..>637
                     /locus_tag="ACHXOX_004640"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          152 a          143 c          209 g          133 t
ORIGIN      
        1 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
       61 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
      121 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
      181 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
      241 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
      301 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
      361 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
      421 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
      481 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgacccttt
      541 aagggtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
      601 gatgcgttga gctaaccggt actaatgaac cgtgagg
//
LOCUS       DAZRVY010000402          634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZRVY010000402 DAZRVY010000000
VERSION     DAZRVY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.405"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>634)
                     /locus_tag="ACHXOX_004641"
     CDS             complement(<1..>634)
                     /locus_tag="ACHXOX_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR7007889.1"
                     /translation="VISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSC
                     ISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSL
                     VLGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVK
                     GNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDEL"
BASE COUNT          171 a          150 c          112 g          201 t
ORIGIN      
        1 taagttcatc agggacatcg caaacaatat gaagacggat ttcttctctg ccgtgactct
       61 tttcactgat tgcgtaactg tcatgctctg gattatttaa ttctttcagc ggaaattttt
      121 cctcaaaggc tttattaagc cgcccctggt ttccttttac cgcgaataaa taatcaccgc
      181 cctgtttttg tatcttctct gcaatatctt tctggcatcc catcgcatca gttgtgatga
      241 tttttccttt aatatccagc atgttaagaa gttcaggaat ggctgtaatc tcattggatt
      301 tctcatccgt cttaatctgc ccgagaacca gactgtgcat tgttgagaac gcactaatga
      361 catgaatcgc tcccttgcgg cgactcttgt cataagagtg ccggagcgtt tttccatcaa
      421 ttgcaatgac gtctttatca tctgaagaat ggcagtcacg catccagtta ataaagcact
      481 cgtgaaattt tgcaggactg atacaggata caactctggc aatagtatcg tgaacaggaa
      541 taccattttc aaaatcacca tattgcttca aaaaatcgag atgtgtttca ccaaaatcct
      601 ctatatcttc ccaaccctct gcaccagaaa tgac
//
LOCUS       DAZRVY010000403          627 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZRVY010000403 DAZRVY010000000
VERSION     DAZRVY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.406"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            460..>627
                     /locus_tag="ACHXOX_004642"
     CDS             460..>627
                     /locus_tag="ACHXOX_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR7007890.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          179 a          109 c          140 g          199 t
ORIGIN      
        1 tagcacgtcg tatgcaagaa cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt
       61 tgaatgaaaa tcagccggag atgattttac ataattgcta cggaattatt caatacagga
      121 attgcttgcg tctgcatgga ttgacctgaa atattcccga aaatttctct aaaaaactcg
      181 aaaaaaatgg taactggttg aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag
      241 atgaacgtaa atttatttaa cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa
      301 caggaatcgt gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac
      361 tttatagtca accataacac acactctact gtctgagtcc aacgtttttt aacattcttg
      421 ttaaaattat gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt
      481 tatcagagaa ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt
      541 tgtcttgagc agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta
      601 cttatctcag tgggagacgt tatcgat
//
LOCUS       DAZRVY010000404          626 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZRVY010000404 DAZRVY010000000
VERSION     DAZRVY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.407"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>626
                     /locus_tag="ACHXOX_004643"
     CDS             <1..>626
                     /locus_tag="ACHXOX_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7007891.1"
                     /translation="NDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGNAQQTAQD
                     VAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATM
                     EAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPY
                     DIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRY"
BASE COUNT          156 a          168 c          182 g          120 t
ORIGIN      
        1 aatgactttc tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt
       61 gaggaaatgg tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag
      121 acggcgcagg atgtggcggc agccgcaggt tatgcccgcg cagcagagca ggccaaaaat
      181 gacattgatg ctgcgctgac cggcactctg aaaacggcta accatctgtc tgaaatcgca
      241 gcagcaggcg aaaaggcaca acagaagtcc cgggataatc tggggctgaa aagtgcggcc
      301 acgatggaag cacagagcga catttacgac cggacaaaag gccgtctggc gatacccggc
      361 gcattcggct ttgggtgtgc ttttctgcct gaagatgtta tccgttttga cactaagagt
      421 gatttcctgg cctgggtaag gaatgcgctg ccaggtgaat attccgttgc tggcccctac
      481 gacatcatca tacccgacac acggtttgaa ggggtgctca gcatccggtg gactgatgca
      541 cgccctgaga caacagaacc gaggtacaga gccaaatccc ttacttttta cggcattaac
      601 ggccccattt atcacacccg ctactg
//
LOCUS       DAZRVY010000405          624 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZRVY010000405 DAZRVY010000000
VERSION     DAZRVY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.408"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>624)
                     /locus_tag="ACHXOX_004644"
     CDS             complement(<1..>624)
                     /locus_tag="ACHXOX_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007892.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          109 a          227 c          191 g           97 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgaacctgcg tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaaacct gagaagagga aacagaacgg ggactggccg gggaagataa
      301 ggaagaggcg cgctgtttac gggacgcttc cgcccgctgc gcctgcgtgc tcagcgccat
      361 gtgaaaacgg cggcccgcgc cgtccgtcac ccccgtcacc gcgcccgcca cgtcaccctc
      421 cgccgcccgg tggaaagtca gtgagcgccc gaagccatcc accacgaccg tcagtacccg
      481 gtatgcgggc ggctccggcc agctgagtat ccaccacggc ccctgaaggc tgtttgtcgc
      541 caggtacaca tgcggactca ggcgaacatc ttccggcagc acctgccaga gcgcgcttag
      601 cggctgcgaa ctgtgctgcg ccgc
//
LOCUS       DAZRVY010000406          596 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZRVY010000406 DAZRVY010000000
VERSION     DAZRVY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.409"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            112..>596
                     /locus_tag="ACHXOX_004645"
     CDS             112..>596
                     /locus_tag="ACHXOX_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR7007893.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT          135 a          160 c          156 g          145 t
ORIGIN      
        1 ttttaaaaac tggcatccgc tttgcaaaca agccaatacg cggctgaaaa ggatatgcag
       61 gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt
      121 ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac
      181 atgccgacct ttgaactgaa taaagccatt gtgcagaaag cggagcatta ccatttcgat
      241 ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac
      301 aaccttgagt cgttcacctt gatggcgggt ctggcagccg tgacctcgcg cattcagatt
      361 tacgccactg ctgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc
      421 atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagccc
      481 gtgtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat
      541 ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cggggaaaag cgattt
//
LOCUS       DAZRVY010000407          595 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZRVY010000407 DAZRVY010000000
VERSION     DAZRVY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.410"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(147..>595)
                     /locus_tag="ACHXOX_004646"
     CDS             complement(147..>595)
                     /locus_tag="ACHXOX_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7007894.1"
                     /translation="DEAVSGSVKASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDV
                     LSTVKALQAKGVTVISLREGFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAK
                     AEGVHMGRPVKASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          164 a          159 c           96 g          176 t
ORIGIN      
        1 ccccgaatct tcgaaaatat ctgtctagtt gattcggtag aatctacctc atgatgtata
       61 cgtgtttagt acccgtcaca ccagctatgt gacccccttg gcaaaaaagc caaatatcag
      121 ctacatccat gtaactattc agtaacttat ttagcaagtc tgtaataagt tgctctagag
      181 attcctagct cttcctgaat ctgcaacctc gttttacctt gtgaaataag catctgtaca
      241 gcctctgaag aagcttttac aggtcttccc atatgtaccc cttcagcttt agcacgttca
      301 ataccagcct ttctacgctc tgctatcaag cttctttcaa gctgtgccaa cgttgacata
      361 atgcctaaca ttgcttcacc catagcgctt gataagtcaa atccttctct aaggctgata
      421 actgtcacac ctttagcctg tagcgcttta accgttgata acacatcaat tgtgtcacgt
      481 ccaagacggt ctacagctac cacaactacg gtgtcacctt ctcgtgcata agctaacaaa
      541 ctgctgaaac cgtttcttaa agaagctttg acagaaccac tgacagcttc atcag
//
LOCUS       DAZRVY010000408          592 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZRVY010000408 DAZRVY010000000
VERSION     DAZRVY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.411"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHXOX_004647"
     CDS             <1..91
                     /locus_tag="ACHXOX_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007895.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>592
                     /gene="mcbR"
                     /locus_tag="ACHXOX_004648"
     CDS             289..>592
                     /gene="mcbR"
                     /locus_tag="ACHXOX_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR7007896.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          171 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tg
//
LOCUS       DAZRVY010000409          581 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZRVY010000409 DAZRVY010000000
VERSION     DAZRVY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.412"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..502
                     /locus_tag="ACHXOX_004649"
     CDS             <1..502
                     /locus_tag="ACHXOX_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR7007897.1"
                     /translation="ISDPNEPKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGT
                     PCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQH
                     NDSNNETGEDELNWKRQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSD
                     ERLPDF"
     gene            480..>581
                     /locus_tag="ACHXOX_004650"
     CDS             480..>581
                     /locus_tag="ACHXOX_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR7007898.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADD"
BASE COUNT          196 a          119 c          155 g          111 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg aagaaatggc atgggctcgt cactaccagc
      541 agatcgttcg tgaagaaaaa gaaacggaac tggcggacga c
//
LOCUS       DAZRVY010000410          561 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZRVY010000410 DAZRVY010000000
VERSION     DAZRVY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.413"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>561)
                     /locus_tag="ACHXOX_004651"
     CDS             complement(<1..>561)
                     /locus_tag="ACHXOX_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR7007899.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          120 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact t
//
LOCUS       DAZRVY010000411          559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZRVY010000411 DAZRVY010000000
VERSION     DAZRVY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.414"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHXOX_004652"
     CDS             <1..428
                     /locus_tag="ACHXOX_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR7007900.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAZRVY010000412          557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZRVY010000412 DAZRVY010000000
VERSION     DAZRVY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.415"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHXOX_004653"
     CDS             <1..>557
                     /locus_tag="ACHXOX_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7007901.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZRVY010000413          557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZRVY010000413 DAZRVY010000000
VERSION     DAZRVY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.416"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHXOX_004654"
     CDS             <1..>557
                     /locus_tag="ACHXOX_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7007902.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZRVY010000414          555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZRVY010000414 DAZRVY010000000
VERSION     DAZRVY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.417"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            78..555
                     /locus_tag="ACHXOX_004655"
                     /pseudo
     CDS             78..555
                     /locus_tag="ACHXOX_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAZRVY010000415          555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZRVY010000415 DAZRVY010000000
VERSION     DAZRVY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.418"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>555
                     /gene="feaR"
                     /locus_tag="ACHXOX_004656"
     CDS             <1..>555
                     /gene="feaR"
                     /locus_tag="ACHXOX_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR7007903.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVAQY"
BASE COUNT          142 a          125 c          146 g          142 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca atata
//
LOCUS       DAZRVY010000416          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZRVY010000416 DAZRVY010000000
VERSION     DAZRVY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.419"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..550
                     /locus_tag="ACHXOX_004657"
                     /pseudo
     CDS             1..550
                     /locus_tag="ACHXOX_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          133 a          137 c          162 g          118 t
ORIGIN      
        1 ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt ggatgccggg
       61 caactgcacg cattatgggc gttggcctca acacgatttt acgtcactta aaaaactcag
      121 gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat
      181 ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt tttacgcgta
      241 tgacagtctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct
      301 ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg
      361 gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca
      421 gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc
      481 gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt atctgaacat
      541 aaaacactat
//
LOCUS       DAZRVY010000417          548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZRVY010000417 DAZRVY010000000
VERSION     DAZRVY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.420"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHXOX_004658"
     CDS             <1..516
                     /locus_tag="ACHXOX_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR7007904.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          159 a          122 c          144 g          123 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgac
//
LOCUS       DAZRVY010000418          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZRVY010000418 DAZRVY010000000
VERSION     DAZRVY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.421"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHXOX_004659"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHXOX_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZRVY010000419          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZRVY010000419 DAZRVY010000000
VERSION     DAZRVY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.422"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHXOX_004660"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHXOX_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZRVY010000420          540 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZRVY010000420 DAZRVY010000000
VERSION     DAZRVY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.423"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            192..>540
                     /locus_tag="ACHXOX_004661"
     CDS             192..>540
                     /locus_tag="ACHXOX_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR7007905.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAE"
BASE COUNT          123 a          150 c          144 g          123 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
//
LOCUS       DAZRVY010000421          539 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZRVY010000421 DAZRVY010000000
VERSION     DAZRVY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.424"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          172 a           58 c          101 g          208 t
ORIGIN      
        1 aattatctaa aagttgccgt tctactccgt tagtttgtag ttctatcata gtcttattaa
       61 ctcgatcacg aggtctgtat gacggggaag cttgcgctgt aagattatat gataatgcac
      121 atattgtaat gatagttgtt tttatctttg tgtttaatgt gaaaatttta taaagcatat
      181 aatgttccct atttttattt tgtttataat ttattggttg tatgttaaat ctatcataag
      241 tctaatgatt aaaatggtta gtggtgtcag gattagtgaa aataaaggat gtaatatgct
      301 attctttctc tgtaagtact tgagaatact aaaccatagt acaagaacga ttatgtacat
      361 gtagtgagaa ttggaaaaca tagtttcttc ccgtgttaat gagagtatgt gtttttatgt
      421 ggggatatgt acaccccata tagatgtatt atgttatatt tataatatac ctcggcaaaa
      481 taaaaggatt gttatagaaa tagggaagct ataaaaaagc ttatattaag gtctgtttt
//
LOCUS       DAZRVY010000422          530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZRVY010000422 DAZRVY010000000
VERSION     DAZRVY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.425"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..333
                     /locus_tag="ACHXOX_004662"
     CDS             <1..333
                     /locus_tag="ACHXOX_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007906.1"
                     /translation="TSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSAGGD
                     CANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCGSGV
                     FSVFGYQT"
     gene            complement(330..>530)
                     /locus_tag="ACHXOX_004663"
     CDS             complement(330..>530)
                     /locus_tag="ACHXOX_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFR7007907.1"
                     /translation="ALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVESPS
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          128 c          121 g          145 t
ORIGIN      
        1 acttccggtt cctctaacgg tagttacagt aatcttggct cacacagagg atcatttact
       61 ggtcgcaata caggaagtgg tacgctgttt gtttacgcct ctggagggaa tggtggttcc
      121 gccgggggag attgcgctaa cacatccaga ttgcagggat acgttgcagg ggcattgatt
      181 agcacaaatg cgagcaacaa cccatcttat gggaagacag cattcatctc ttttgctgtc
      241 cctgctggcg caacttatca gatcacctct tatccggccc aaaactactc ttgtggctca
      301 ggggtatttt cagtatttgg ataccaaact taattgagag ttacacagga cgcaatacaa
      361 cctgtcttgt gctcgtattt gtaccatttt cgtgaaccag atggactctc gactacctgg
      421 cagtaatgag aatcctcagc atttcccatt ctggcaatgg cacaaaagtc aaattgcccc
      481 aagtaaccag tgctggaaag ttgagtaacc ttgagctttc cacctaatgc
//
LOCUS       DAZRVY010000423          522 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZRVY010000423 DAZRVY010000000
VERSION     DAZRVY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.426"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>522)
                     /locus_tag="ACHXOX_004664"
     CDS             complement(<1..>522)
                     /locus_tag="ACHXOX_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007908.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAZRVY010000424          517 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZRVY010000424 DAZRVY010000000
VERSION     DAZRVY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.427"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(69..>517)
                     /locus_tag="ACHXOX_004665"
     CDS             complement(69..>517)
                     /locus_tag="ACHXOX_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7007909.1"
                     /translation="DEAVSGSVKASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDV
                     LSTVKALQAKGVTVISLREGFDLSSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAK
                     AEGVHMGRPVKASSEAVQTLISQGKTRLQIQEELGISRATYYRLAK"
BASE COUNT          142 a          144 c           83 g          148 t
ORIGIN      
        1 caccagctat gtgaccccct tggcaaaaaa gccaaatatc agctacatcc atgtaactat
       61 tcagtaactt atttagcaag tctgtaataa gttgctctag agattcctaa ctcttcctga
      121 atctgcaacc tcgtcttacc ttgtgaaata agcgtctgta cagcctctga agaagctttt
      181 acaggcctcc ccatatgtac cccttcagct ttagcacgtt caataccagc ctttctacgc
      241 tctgctatca agcttctttc aagctgtgcc aacgttgaca taatgcctaa cattgcttca
      301 cccatagcgc ttgataagtc aaacccttct ctaaggctga taactgtcac acctttagcc
      361 tgtagcgctt taaccgttga taacacatca attgtgtcac gtccaagacg gtctacagct
      421 accacaacta cggtgtcacc ttctcgtgca taagctaata gtctgctgaa accgtttctt
      481 aaagaagctt tgacagaacc actgacagct tcatcag
//
LOCUS       DAZRVY010000425          514 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZRVY010000425 DAZRVY010000000
VERSION     DAZRVY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.428"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOX_004666"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHXOX_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          154 a          126 c          105 g          129 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataa
//
LOCUS       DAZRVY010000426          511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZRVY010000426 DAZRVY010000000
VERSION     DAZRVY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.429"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..511
                     /locus_tag="ACHXOX_004667"
                     /pseudo
     CDS             1..511
                     /locus_tag="ACHXOX_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          128 a          127 c          145 g          111 t
ORIGIN      
        1 attgaaatgg agaatgaaat attaaaaaag gctaccgcgc tcttgatgtc agactccctg
       61 aacagttctc gataatcggg aaactcagag cgcattatcc tgtggtcaca ctctgccatg
      121 tgttcggggt tcatcgcagc agctacagat actggaaaaa ccgtcctgaa aaaccagacg
      181 gcagacgggc tgtattacgc agtcaggtac ttgagctaca tggcatcagc cacggttcgg
      241 ccggagcaag aagcatcgcc acaatggcaa cccggagagg ctaccagatg ggacgctggc
      301 ttgctggcag gctcatgaaa gagctggggc tggtcagctg tcagcagccg actcaccggt
      361 ataaacgtgg tggtcatgaa catgttgcta tccctaacta ccttgaaagg cagttcgccg
      421 tgaccgagcc aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct
      481 gggcgtacct cgccgttgtt ctcgacctgt t
//
LOCUS       DAZRVY010000427          489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZRVY010000427 DAZRVY010000000
VERSION     DAZRVY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.430"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXOX_004668"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXOX_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR7007910.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZRVY010000428          485 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZRVY010000428 DAZRVY010000000
VERSION     DAZRVY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.431"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>485)
                     /locus_tag="ACHXOX_004669"
     CDS             complement(<1..>485)
                     /locus_tag="ACHXOX_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR7007911.1"
                     /translation="IATVASMLLMFPWDIAAITVSQRLANGLDNFALLAIPFFIFAGT
                     LMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLANMMFGSISGSAVAAAAAVGGTLNPI
                     QTKQGYDPAFSTAVNVSSCITGLLIPPSNVLIVFSLTAGGVSVASLFMAGYLPGILMG
                     LA"
BASE COUNT          157 a          121 c          115 g           92 t
ORIGIN      
        1 gcaagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc tacagaaacg
       61 ccgccagctg tcagggaaaa cacaattaac acgtttgagg gcggtatcag taatccagta
      121 atacaagatg agacgtttac agcggtagaa aatgccggat cataaccttg tttggtctga
      181 atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc agaaatggaa
      241 ccaaacatca tattcgccag gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc
      301 attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat cagcgttccg
      361 gcaaagatga aaaaggggat tgccaatagg gcaaagttat ccagtccatt agccagtcgc
      421 tgcgaaacag tgattgccgc gatatcccag ggaaacatca aaagcattga agctacagtg
      481 gcaat
//
LOCUS       DAZRVY010000429          480 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZRVY010000429 DAZRVY010000000
VERSION     DAZRVY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.432"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            30..>480
                     /locus_tag="ACHXOX_004670"
     CDS             30..>480
                     /locus_tag="ACHXOX_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR7007912.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          117 a          116 c          123 g          124 t
ORIGIN      
        1 accccggtat ttccaccacg gaggccagca tgaacaacaa taacacattg tacgtcgggt
       61 tagatgttca caaagaatcg attactgtcg cttatgctat caattcagaa cctgttgaac
      121 tgatgggtaa aattggcaca tcacctactg atattcagaa tctttgtaaa cgtctcaggt
      181 caaagtcatc gcaggtcagt atcgtttatg aagcggggcc ctgtggttat ggactttatc
      241 gtcggctggt gaaatccggt tttgactgca tggtctgcgc cccctcactt atcccgaaaa
      301 aaccggggga gcgggttaaa accgatcgcc gtgatgccat cagacttgtg cgttcactgc
      361 gtgcaggaga tctttctgct gtctacgtac ccggcattga agatgaggca ttccgggatt
      421 tggcccgggc atgggcatct gcccgcgatg atttacggca tgcaaggcaa cgtctgaaat
//
LOCUS       DAZRVY010000430          476 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZRVY010000430 DAZRVY010000000
VERSION     DAZRVY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.433"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHXOX_004671"
     rRNA            <1..>476
                     /locus_tag="ACHXOX_004671"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZRVY010000431          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZRVY010000431 DAZRVY010000000
VERSION     DAZRVY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.434"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHXOX_004672"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHXOX_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZRVY010000432          469 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZRVY010000432 DAZRVY010000000
VERSION     DAZRVY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.435"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..298
                     /locus_tag="ACHXOX_004673"
                     /pseudo
     CDS             1..298
                     /locus_tag="ACHXOX_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          193 a           75 c           63 g          138 t
ORIGIN      
        1 atcgaaggtt tattttcaac attattaacc attaatgaca actttttacg agcaaaagcg
       61 gaaattgctt cgacaataat taaattttta gaggccagga taaccaatct ttcatacaat
      121 gacatattaa aatatcagca agaattccaa aaacaatgtt ataagcaagt taaagccttt
      181 acgacactct ctcgatacaa taaaattcaa acatgggcag aaatgtctga atatcagttc
      241 gaagtatttc aatacgagac gcttaacccc aaaaaaatgt cacacacgcc ttatttaaag
      301 aggccacttc ctaatgaaaa agatataaat tatggggtgg aaatagaaat accatctggc
      361 aaaagaattc gcttatcaaa tcattatcag aatataatac cataatattg attattagca
      421 ctttataatt attgaatgaa aatagatttt atgtaatttt aaaacaaag
//
LOCUS       DAZRVY010000433          467 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZRVY010000433 DAZRVY010000000
VERSION     DAZRVY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.436"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHXOX_004674"
     CDS             30..206
                     /locus_tag="ACHXOX_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007913.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          167 a           55 c          108 g          137 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcagaagtt cactttttgt actaaataat tcgcatt
//
LOCUS       DAZRVY010000434          465 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZRVY010000434 DAZRVY010000000
VERSION     DAZRVY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.437"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>465
                     /locus_tag="ACHXOX_004675"
     CDS             <1..>465
                     /locus_tag="ACHXOX_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR7007914.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT          119 a          108 c          150 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactg
//
LOCUS       DAZRVY010000435          464 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZRVY010000435 DAZRVY010000000
VERSION     DAZRVY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.438"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHXOX_004676"
     CDS             <1..279
                     /locus_tag="ACHXOX_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7007915.1"
                     /translation="ELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVD
                     PDLIWSLPDGEIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            280..>464
                     /locus_tag="ACHXOX_004677"
     CDS             280..>464
                     /locus_tag="ACHXOX_004677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007916.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          132 a           97 c          119 g          116 t
ORIGIN      
        1 gaactggtcg caagtggtga tgtatataat tctggctacg gcttattccc gtctgaacag
       61 gctcgcaagg actggcaaag cgcccgcaaa aaactctcga gagtaaaggt gaggaaaccg
      121 gttgttgttg atccggacct tatctggtca ttacctgacg gagaaatacg ccgctacgac
      181 aggcgcctga atataatctg tcgcgagtgc cggaagagcg aagctatgca gcgtgtactg
      241 gctttctatc agggtaattt tcaggaggcg atactgtgaa tgaaattagc tatcaggctt
      301 caattaccgc tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag
      361 ttaagcaata tcgttttatt tccggtaaaa gaggatgacc ctcgtaatca ggttaatttt
      421 ctttatgagc catcggaaag accatattgc catcacgcct ctgt
//
LOCUS       DAZRVY010000436          456 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZRVY010000436 DAZRVY010000000
VERSION     DAZRVY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.439"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHXOX_004678"
     CDS             2..388
                     /locus_tag="ACHXOX_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7007917.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           95 c          122 g          104 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagca
//
LOCUS       DAZRVY010000437          454 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZRVY010000437 DAZRVY010000000
VERSION     DAZRVY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.440"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHXOX_004679"
     CDS             <1..118
                     /locus_tag="ACHXOX_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR7007918.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNYEKLQSS"
     gene            225..>454
                     /locus_tag="ACHXOX_004680"
     CDS             225..>454
                     /locus_tag="ACHXOX_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR7007919.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSP"
BASE COUNT          160 a           93 c           98 g          103 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattacgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgtt
//
LOCUS       DAZRVY010000438          448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZRVY010000438 DAZRVY010000000
VERSION     DAZRVY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.441"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..238)
                     /locus_tag="ACHXOX_004681"
     CDS             complement(<1..238)
                     /locus_tag="ACHXOX_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR7007920.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLY"
BASE COUNT          127 a           88 c          119 g          114 t
ORIGIN      
        1 agtacagcat gtcaggcata acaagctctg acgcagaggt gtctttgggc gggaaaatga
       61 gtttctccag cgtgtcatgg gatatatccg caggtaatgg ccatgacaga gaagaggctt
      121 tgaagcgggc aagaacctca aaaacggttg aggcgctgat gtgtaacgtc agtccgatac
      181 aacggttagt cagcttctta tcgaacttga gctgaaggat cttaaaacaa gtttccatag
      241 tgctcctgga ttttctcttt ttggacattg acgtctccac tggtttaaac acggcaatgg
      301 agactgtggt gaaaagagtt aattcccgga gtgactggcg aattccaatc aatgatcgga
      361 agtatgccaa atcgtgatcg attgtgatgc caaactgtga tcggagatgg atgccaaatc
      421 atgatcgatt cgaatgccaa actgcgat
//
LOCUS       DAZRVY010000439          442 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZRVY010000439 DAZRVY010000000
VERSION     DAZRVY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.442"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHXOX_004682"
     CDS             <1..>442
                     /locus_tag="ACHXOX_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7007921.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZRVY010000440          435 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZRVY010000440 DAZRVY010000000
VERSION     DAZRVY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.443"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>435
                     /locus_tag="ACHXOX_004683"
     CDS             <1..>435
                     /locus_tag="ACHXOX_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7007922.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcg
//
LOCUS       DAZRVY010000441          426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZRVY010000441 DAZRVY010000000
VERSION     DAZRVY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.444"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHXOX_004684"
     CDS             complement(<1..112)
                     /locus_tag="ACHXOX_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007923.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
BASE COUNT          122 a           88 c           86 g          130 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgt
//
LOCUS       DAZRVY010000442          420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZRVY010000442 DAZRVY010000000
VERSION     DAZRVY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.445"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHXOX_004685"
     tRNA            41..116
                     /locus_tag="ACHXOX_004685"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZRVY010000443          409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZRVY010000443 DAZRVY010000000
VERSION     DAZRVY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.446"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>409)
                     /locus_tag="ACHXOX_004686"
     CDS             complement(<1..>409)
                     /locus_tag="ACHXOX_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007924.1"
                     /translation="HCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSKVNFDTQQMIHF
                     INRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETE
                     EKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          133 a           70 c           65 g          141 t
ORIGIN      
        1 ggagcttacc accataacag gcccatcatc gacagaggag aacaggataa atatattatc
       61 gtttcgtgtt aatttattag tgttaaaggt tcttaacttt tcttcagttt ctataaatat
      121 aggtgaaaca tcatcactac gaatccactc tttataaaga taccaggcat ttgctttgat
      181 catatcatcc gttgatcgta aggcctgatc gataagttgg ataaaaaaat tgttattttt
      241 atcaatcagc cctttattca tattaaatct gttaataaaa tggatcatct gttgggtatc
      301 gaaattcacc ttactgaatc gatgttgacg cataaaatta tcgaacttgt catcttcccc
      361 agaatgtata taactacgta taattggatc ttttatataa ggacaatgg
//
LOCUS       DAZRVY010000444          408 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZRVY010000444 DAZRVY010000000
VERSION     DAZRVY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.447"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHXOX_004687"
     tRNA            54..130
                     /locus_tag="ACHXOX_004687"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHXOX_004688"
     tRNA            173..248
                     /locus_tag="ACHXOX_004688"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZRVY010000445          403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZRVY010000445 DAZRVY010000000
VERSION     DAZRVY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.448"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>403
                     /gene="istB"
                     /locus_tag="ACHXOX_004689"
     CDS             <1..>403
                     /gene="istB"
                     /locus_tag="ACHXOX_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR7007925.1"
                     /translation="LLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQG
                     RVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPV
                     DKWHGLMENPTTADAILDRLVHNSHRVVLQG"
BASE COUNT          120 a           87 c          116 g           80 t
ORIGIN      
        1 acctactgat aaccggtccg acaggatgcg gtaagagctg gatagccaat gcgctgggcg
       61 aacaggcatg tcgacagaaa tacagcgtcc ggtactgtag aaccgggcga ttactggaac
      121 agttggctca gggacgtgtt gatggcagtt ggctgaaata tctgaaacag ttgcaaaaaa
      181 tacaggttct aatcctggac gatctggggc tggaacagtt aagtaacgcc cagtgtaatg
      241 accttctgga gatcacagag gatcgttacg gacaaagcag cacgatagtg gtaagccagt
      301 tcccggtaga taaatggcat ggtctgatgg agaacccaac aacagcagat gccatcctgg
      361 acagactggt acataactca cacagagtgg tccttcaggg aga
//
LOCUS       DAZRVY010000446          403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZRVY010000446 DAZRVY010000000
VERSION     DAZRVY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.449"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..83)
                     /locus_tag="ACHXOX_004690"
     CDS             complement(<1..83)
                     /locus_tag="ACHXOX_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007926.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIG"
     gene            complement(83..>403)
                     /locus_tag="ACHXOX_004691"
     CDS             complement(83..>403)
                     /locus_tag="ACHXOX_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR7007927.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          139 a           89 c           79 g           96 t
ORIGIN      
        1 ccgattgaga acgaaatggg cacaccaata aagaccagca caaaaaaaca gccaaacagt
       61 gtaatcgcga tataactatc cattaactgg cctcctgcgc aggttgtttc agttgttgaa
      121 tgctttgatt aataaaacac aaggcataaa acatcatcac caatccactt aatggcagaa
      181 tgatatatac gtatcccatc ggtatttgca tcgcggcaga tacctgtgat gttgccagcg
      241 ttttatcaat taatttcagg ccaccagtca caatgaccga accagcaaaa cctaaaataa
      301 gaacgttaat gactatacta agaagtaatt gcttgcgctt attcagggcg agagcaaata
      361 aatcgataga gagatgtctc tgcgcgccga cggtatatgc agc
//
LOCUS       DAZRVY010000447          398 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZRVY010000447 DAZRVY010000000
VERSION     DAZRVY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.450"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>398
                     /locus_tag="ACHXOX_004692"
     CDS             <1..>398
                     /locus_tag="ACHXOX_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR7007928.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          105 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatga
//
LOCUS       DAZRVY010000448          391 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZRVY010000448 DAZRVY010000000
VERSION     DAZRVY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.451"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>391)
                     /locus_tag="ACHXOX_004693"
     CDS             complement(<1..>391)
                     /locus_tag="ACHXOX_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7007929.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           71 a          135 c          113 g           72 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcattttc c
//
LOCUS       DAZRVY010000449          387 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZRVY010000449 DAZRVY010000000
VERSION     DAZRVY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.452"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          116 a           72 c           76 g          123 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgct
//
LOCUS       DAZRVY010000450          380 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZRVY010000450 DAZRVY010000000
VERSION     DAZRVY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.453"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>380
                     /locus_tag="ACHXOX_004694"
     CDS             <1..>380
                     /locus_tag="ACHXOX_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR7007930.1"
                     /translation="DCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVE
                     WLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPD
                     GLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT           98 a           91 c          119 g           72 t
ORIGIN      
        1 tggactgctg tgatcgtgag gcactgcact gggcggtgac taccggcggc ttcaacagtg
       61 aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg cttcggcaac gatcttccgt
      121 cgtctccagt ggagtggctg acggataatg gttcatgcta ccgggctaat gaaacacgcc
      181 agttcgcccg gatgttggga cttgaaccga agaacacggc ggtgcggagt ccggagagta
      241 acggaatagc agagagcttc gtgaaaacga taaagcgtga ctacatcagt atcatgccca
      301 aaccagacgg gttaacggca gcaaagaacc ttgcagaggc gttcgagcat tataacgaat
      361 ggcatccgca tagtgcgctg
//
LOCUS       DAZRVY010000451          370 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZRVY010000451 DAZRVY010000000
VERSION     DAZRVY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.454"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..108
                     /locus_tag="ACHXOX_004695"
                     /pseudo
     CDS             1..108
                     /locus_tag="ACHXOX_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            138..>370
                     /locus_tag="ACHXOX_004696"
     CDS             138..>370
                     /locus_tag="ACHXOX_004696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007931.1"
                     /translation="MYICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFS
                     LTLEFNDTIIESQKVTGNKAKYILEDIEIFYRN"
BASE COUNT          137 a           47 c           65 g          121 t
ORIGIN      
        1 ttttatacat agaaacacag ttaaaaattg caaaagattt ttgtagacct ggagaagaag
       61 tatcaagttc tattgcaagt aaagtttttc acgcatttat cgatttagtt aacaaaatca
      121 ggggtaagaa agactttatg tatatttgca cgctttgctg ttttgccgag gaggttaaag
      181 gtgattattc tcattacagg acctttttat ttgatattgg taatcaatat aaggttaaac
      241 ttacacaaag cggaaaaaaa gagttctctt taacattaga atttaacgat actataattg
      301 aatctcagaa agtcacaggc aataaggcaa agtatattct tgaggatata gaaatattct
      361 atcgtaacaa
//
LOCUS       DAZRVY010000452          369 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZRVY010000452 DAZRVY010000000
VERSION     DAZRVY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.455"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>369
                     /locus_tag="ACHXOX_004697"
     CDS             <1..>369
                     /locus_tag="ACHXOX_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007932.1"
                     /translation="ISTGNINVNCPLKNIYNEIRRLNVIFSCGTGDIVDLSSLDLRNV
                     DLDYYDFTDKHMANTILNPFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPVL
                     ISISDMYSEEKIKFVESCLN"
BASE COUNT          130 a           53 c           57 g          129 t
ORIGIN      
        1 aatatctaca ggcaatatca atgttaattg cccattaaaa aatatttaca atgaaatcag
       61 gaggttgaac gtaattttta gttgtgggac tggagatata gttgatctat cctctctgga
      121 cttacgtaat gtcgatttag attattacga ttttacagat aaacatatgg ctaatactat
      181 tttaaatcct tttaaattgg attcaacaaa ttttactaat gccaacatgt tccaggttaa
      241 ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa aaataacgcc
      301 tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg
      361 tttaaatga
//
LOCUS       DAZRVY010000453          363 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZRVY010000453 DAZRVY010000000
VERSION     DAZRVY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.456"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            163..363
                     /locus_tag="ACHXOX_004698"
                     /pseudo
     CDS             163..363
                     /locus_tag="ACHXOX_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          116 a           89 c           79 g           79 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcaaccttca cgataaaaaa
      181 cttttcatta aaggtaaagc ggatcaatat ccggcacttc attctctgat atctccgacc
      241 cgcattaatc tgaaagaaat agaaattcac tggcgtgaag tgctacgtct ggcgacctca
      301 ataaaacagg gaacagtgac agcatccctg atgctgagaa aacttgccag ttaccccaaa
      361 caa
//
LOCUS       DAZRVY010000454          357 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZRVY010000454 DAZRVY010000000
VERSION     DAZRVY010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.457"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHXOX_004699"
     rRNA            <1..>357
                     /locus_tag="ACHXOX_004699"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZRVY010000455          353 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZRVY010000455 DAZRVY010000000
VERSION     DAZRVY010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.458"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT          105 a           71 c           68 g          109 t
ORIGIN      
        1 tacccccccg ttcccagaga aataatattt attaaaattc cagttcttct ttttctgatt
       61 acagaaggca aagtggcaat tacgcatagt ttcccgataa agacgcgata gcgacatccc
      121 gcataaggca tttttctctt tatctttgta cggtacttca tggaacagag tttttgacct
      181 tgcgaatcgt gatgtctgtt ggggaggggc aatttgctca ctgaagcgtg agactcgatt
      241 aagcgcacga aacacagaaa tcaaaaaacc cggtcacttt tttacaaggt aaccgggtaa
      301 aaataatttt tattttttaa ctgttttgag actcatagag atgtctcaaa act
//
LOCUS       DAZRVY010000456          348 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZRVY010000456 DAZRVY010000000
VERSION     DAZRVY010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.459"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            109..309
                     /gene="glgS"
                     /locus_tag="ACHXOX_004700"
     CDS             109..309
                     /gene="glgS"
                     /locus_tag="ACHXOX_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR7007933.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT           98 a           61 c           88 g          101 t
ORIGIN      
        1 aatatattta cgcacgttat gtttaaaggc actacactga ttggggaata ctgaaatcag
       61 aaaaagcata tggccttaaa aggtatgcga taaccggagg atgtgcttat ggatcatagt
      121 cttaattctt taaataattt cgatttcctg gcgcgtagtt ttgccagaat gcacgcagaa
      181 ggtcgcccgg tcgatattct tgccgttact ggtaacatgg atgaagaaca tagaacctgg
      241 ttttgcgcac gttatgcctg gtattgccaa cagatgatgc aggcaagaga gctggagtta
      301 gagcactgat ataacgggcc tgatggcccg ttttagtgtt tgtattag
//
LOCUS       DAZRVY010000457          340 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZRVY010000457 DAZRVY010000000
VERSION     DAZRVY010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.460"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(173..>340)
                     /locus_tag="ACHXOX_004701"
     CDS             complement(173..>340)
                     /locus_tag="ACHXOX_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7007934.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          114 a           57 c           65 g          104 t
ORIGIN      
        1 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
       61 taataagttt gaagacgcaa aaaaataata taataacaaa aatcctccag ccttgtaggc
      121 cagaggataa aatacttaat tgaggaaaat atcagggaaa agtatttctg acttaacttt
      181 cccggctgga gagtgaatca atgtaatcag cgtaccattg catcatttca cggcggccat
      241 cgagatacag ggcatgatta taggtacccc tgatggaatt tttatccaca tgagctaact
      301 gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
//
LOCUS       DAZRVY010000458          340 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZRVY010000458 DAZRVY010000000
VERSION     DAZRVY010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.461"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>340
                     /locus_tag="ACHXOX_004702"
     CDS             <1..>340
                     /locus_tag="ACHXOX_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007935.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEG"
BASE COUNT           82 a          124 c           88 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg
//
LOCUS       DAZRVY010000459          335 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZRVY010000459 DAZRVY010000000
VERSION     DAZRVY010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.462"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>335
                     /locus_tag="ACHXOX_004703"
     CDS             <1..>335
                     /locus_tag="ACHXOX_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR7007936.1"
                     /translation="TEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVY
                     HQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTL
                     RINGKDHDL"
BASE COUNT           94 a           64 c           81 g           96 t
ORIGIN      
        1 tacagaggag attcaacgcg ccgtgattct gaaaatggaa atgatgccat ttttagactc
       61 ggtgggactg gtacttgatg ataataaata ttatctcttt tcgcggaggg cgaatgataa
      121 aatcgttgtt tatcatcagg aacaagtaaa tggaccgctt gtcgacgagt cagggcgggt
      181 tatttttgcc gattttaacc catcgaaacg accgtggtcg gtggcttcag atgactctaa
      241 caacagctgg aatccggcat acaattgctt tgatcgtccg ggtaaaaaat gtatctcttt
      301 tacgctacgt atcaacggca aagatcacga tttgt
//
LOCUS       DAZRVY010000460          332 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZRVY010000460 DAZRVY010000000
VERSION     DAZRVY010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.463"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHXOX_004704"
                     /pseudo
     CDS             complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHXOX_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          112 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aa
//
LOCUS       DAZRVY010000461          331 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZRVY010000461 DAZRVY010000000
VERSION     DAZRVY010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.464"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            84..263
                     /locus_tag="ACHXOX_004705"
                     /pseudo
     CDS             84..263
                     /locus_tag="ACHXOX_004705"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            211..>331
                     /locus_tag="ACHXOX_004706"
     CDS             211..>331
                     /locus_tag="ACHXOX_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR7007937.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET"
BASE COUNT           92 a           88 c           86 g           65 t
ORIGIN      
        1 gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa gagcagcgcg
       61 acaaaaacgc aagagaactg aagctggcga acgcaaccat tactgacatg cagcagcgcc
      121 agcgtgatgc tgatgcactc gatgctaaat acacgaagga gttagctgat gcgaaagctg
      181 aaaatgatgc tcttcggcgc aagcttgata atggtggtcg ggtgctcgtc aaaggaaaat
      241 gccctgtgcc atcctcagcc gaaacctcca gcgcctccgg catgggcaat gatgccaccg
      301 tcgaactctc tccagttgct ggacgaaacg t
//
LOCUS       DAZRVY010000462          328 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZRVY010000462 DAZRVY010000000
VERSION     DAZRVY010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.465"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..328
                     /locus_tag="ACHXOX_004707"
                     /pseudo
     CDS             1..328
                     /locus_tag="ACHXOX_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           84 a           82 c           98 g           64 t
ORIGIN      
        1 tgcagcgaac tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat
       61 ccggataaac gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatacagcgc
      121 gtatacgatg aaaatcacaa ggtatacggt gtaaagtctg gcgtcagttg ttacgggaag
      181 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatggga cttgccggtg
      241 ttctccgggg taaaaaggtc cgtacgacca tcagccggaa agccgttgcc gcaggcgacc
      301 gcgtaaaccg tcagttcgtg gcagaacg
//
LOCUS       DAZRVY010000463          315 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZRVY010000463 DAZRVY010000000
VERSION     DAZRVY010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.466"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>315
                     /locus_tag="ACHXOX_004708"
     CDS             <1..>315
                     /locus_tag="ACHXOX_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7007938.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLV"
BASE COUNT          108 a           45 c           57 g          105 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggt
//
LOCUS       DAZRVY010000464          312 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZRVY010000464 DAZRVY010000000
VERSION     DAZRVY010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.467"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            174..>312
                     /locus_tag="ACHXOX_004709"
     CDS             174..>312
                     /locus_tag="ACHXOX_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR7007939.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT           91 a           59 c           58 g          104 t
ORIGIN      
        1 agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt
       61 tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct
      121 attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc atcatgttta
      181 atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaacct tctttgcagg
      241 cgacggctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa ttacataata
      301 ttcaacgttc tc
//
LOCUS       DAZRVY010000465          308 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZRVY010000465 DAZRVY010000000
VERSION     DAZRVY010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.468"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHXOX_004710"
     CDS             <1..>308
                     /locus_tag="ACHXOX_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7007940.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZRVY010000466          306 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZRVY010000466 DAZRVY010000000
VERSION     DAZRVY010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.469"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHXOX_004711"
     CDS             <1..207
                     /locus_tag="ACHXOX_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007941.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            246..>306
                     /locus_tag="ACHXOX_004712"
     CDS             246..>306
                     /locus_tag="ACHXOX_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR7007942.1"
                     /translation="MEVFMYKITATIEKEGGSPA"
BASE COUNT          106 a           61 c           71 g           68 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgcca
//
LOCUS       DAZRVY010000467          301 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZRVY010000467 DAZRVY010000000
VERSION     DAZRVY010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.470"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHXOX_004713"
     CDS             <1..>301
                     /locus_tag="ACHXOX_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7007943.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZRVY010000468          301 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZRVY010000468 DAZRVY010000000
VERSION     DAZRVY010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.471"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHXOX_004714"
     CDS             <1..>301
                     /locus_tag="ACHXOX_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007944.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZRVY010000469          297 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZRVY010000469 DAZRVY010000000
VERSION     DAZRVY010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.472"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>297)
                     /locus_tag="ACHXOX_004715"
     CDS             complement(<1..>297)
                     /locus_tag="ACHXOX_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7007945.1"
                     /translation="MDKDAQGYIDLSDFDLINCHFKGDVISKVSFLSSNLQHVTFECK
                     EIENCNFTKATVDNVIFKCRRLHNVIFLKTSGECVDFSQNILDTVDFSQSQLGHS"
BASE COUNT          107 a           56 c           41 g           93 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccat
//
LOCUS       DAZRVY010000470          295 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZRVY010000470 DAZRVY010000000
VERSION     DAZRVY010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.473"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHXOX_004716"
     CDS             <1..279
                     /locus_tag="ACHXOX_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR7007946.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT           70 a           77 c           89 g           59 t
ORIGIN      
        1 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
       61 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
      121 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
      181 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
      241 gaactgctcc cctggcgcgt agcactgccg actgaataac acattcccgt caata
//
LOCUS       DAZRVY010000471          293 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZRVY010000471 DAZRVY010000000
VERSION     DAZRVY010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.474"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHXOX_004717"
     CDS             <1..216
                     /locus_tag="ACHXOX_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR7007947.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT           89 a           62 c           61 g           81 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccaa ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctacggtttc ctttacgatt tggggttgcg actttacctc tat
//
LOCUS       DAZRVY010000472          292 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZRVY010000472 DAZRVY010000000
VERSION     DAZRVY010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.475"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHXOX_004718"
     CDS             <1..>292
                     /locus_tag="ACHXOX_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR7007948.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZRVY010000473          289 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZRVY010000473 DAZRVY010000000
VERSION     DAZRVY010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.476"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..192
                     /locus_tag="ACHXOX_004719"
                     /pseudo
     CDS             1..192
                     /locus_tag="ACHXOX_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT           71 a           58 c           97 g           63 t
ORIGIN      
        1 catatggcgt ggggtgatgc cgataatcag taggtgaaac tgtcgatgcc ggaactggaa
       61 gaactggcgg cagcaatggt gcaggctgtt gttgaacgca atgacgagat ttatcgccgt
      121 cagcgtgaaa tgaaagagga gctgagcagt ctggatgatt tggcttcaat tcgggcgttt
      181 gacgttaagt aatgaataag ccgcaactgg cggaatcaca gaagaccgct ttgcttaccg
      241 aggcggagtc tgtcatccgg ccgccggggt gtgcggtcag gctgaacag
//
LOCUS       DAZRVY010000474          284 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZRVY010000474 DAZRVY010000000
VERSION     DAZRVY010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.477"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>284
                     /locus_tag="ACHXOX_004720"
     CDS             <1..>284
                     /locus_tag="ACHXOX_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR7007949.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           88 g           69 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgc
//
LOCUS       DAZRVY010000475          277 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZRVY010000475 DAZRVY010000000
VERSION     DAZRVY010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.478"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHXOX_004721"
     CDS             complement(<1..70)
                     /locus_tag="ACHXOX_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007950.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
BASE COUNT           87 a           63 c           39 g           88 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagaca
//
LOCUS       DAZRVY010000476          275 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZRVY010000476 DAZRVY010000000
VERSION     DAZRVY010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.479"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT           79 a           59 c           37 g          100 t
ORIGIN      
        1 catggtttat tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc
       61 acaactatat tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac
      121 ttcgatgcaa tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct
      181 aatcatcttt agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca
      241 aactcttcag cagggcaaga ttacgaaagc ccgct
//
LOCUS       DAZRVY010000477          274 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZRVY010000477 DAZRVY010000000
VERSION     DAZRVY010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.480"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHXOX_004722"
     tRNA            complement(1..76)
                     /locus_tag="ACHXOX_004722"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHXOX_004723"
     tRNA            complement(79..155)
                     /locus_tag="ACHXOX_004723"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHXOX_004724"
     tRNA            complement(157..233)
                     /locus_tag="ACHXOX_004724"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
BASE COUNT           64 a           74 c           74 g           62 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aact
//
LOCUS       DAZRVY010000478          270 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZRVY010000478 DAZRVY010000000
VERSION     DAZRVY010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.481"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>270)
                     /locus_tag="ACHXOX_004725"
     rRNA            complement(<1..>270)
                     /locus_tag="ACHXOX_004725"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           49 a           82 c           62 g           77 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat
//
LOCUS       DAZRVY010000479          267 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZRVY010000479 DAZRVY010000000
VERSION     DAZRVY010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.482"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>267
                     /locus_tag="ACHXOX_004726"
     CDS             <1..>267
                     /locus_tag="ACHXOX_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR7007951.1"
                     /translation="QFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGY
                     VSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSN"
BASE COUNT           79 a           61 c           74 g           53 t
ORIGIN      
        1 ggcagttccg gcagttattg tggcgatacc agatcagaca gagtatgagc cggcgcggaa
       61 actgctggga taacagccca atggaacgct tcttcaggag tctgaagaac gaatggatgc
      121 cggtggtggg ttacgtaagc ttcagcgagg cagctcacgc cataacggac tatatcgttg
      181 gatattacag cgcactaaga ccgcacgaat ataacggtgg gttaccccca aacgaatcgg
      241 aaaatcgata ctggaaaaac tctaact
//
LOCUS       DAZRVY010000480          259 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZRVY010000480 DAZRVY010000000
VERSION     DAZRVY010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.483"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHXOX_004727"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHXOX_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR7007952.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZRVY010000481          250 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZRVY010000481 DAZRVY010000000
VERSION     DAZRVY010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.484"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..235
                     /locus_tag="ACHXOX_004728"
     CDS             <1..235
                     /locus_tag="ACHXOX_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7007953.1"
                     /translation="YASIQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEER
                     SLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT           71 a           47 c           69 g           63 t
ORIGIN      
        1 gtatgcttcg atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat
       61 ccaggaattt agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac
      121 ggaggaagaa agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt
      181 tgatacatca actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt
      241 gggattatgc
//
LOCUS       DAZRVY010000482          246 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZRVY010000482 DAZRVY010000000
VERSION     DAZRVY010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.485"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>246)
                     /locus_tag="ACHXOX_004729"
     CDS             complement(<1..>246)
                     /locus_tag="ACHXOX_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007954.1"
                     /translation="LTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERT
                     HHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT           35 a           77 c           78 g           56 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagg
//
LOCUS       DAZRVY010000483          246 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZRVY010000483 DAZRVY010000000
VERSION     DAZRVY010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.486"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>246
                     /locus_tag="ACHXOX_004730"
     CDS             <1..>246
                     /locus_tag="ACHXOX_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007955.1"
                     /translation="LMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGSRL
                     EDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTET"
BASE COUNT           85 a           49 c           56 g           56 t
ORIGIN      
        1 tttaatgaat gaaggattct taactaaaaa ggatttgaaa gactttgcca ataactctgc
       61 tggtttaatt tcaaaagctg tcaacggaat ggtgaaaaag ggctataccg acgcacagat
      121 cggtagtcgt ctggaagatt tagcggacga cctcctctta acttctaaat attggcaaag
      181 atcagccaga gaggtgcagg caagcattac ccagcaaaaa gccgccggac aatggctaac
      241 agaaac
//
LOCUS       DAZRVY010000484          244 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZRVY010000484 DAZRVY010000000
VERSION     DAZRVY010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.487"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT           62 a           50 c           66 g           66 t
ORIGIN      
        1 atatttatca tggtgatata aatattttcc taattgtttc tatctgatgg atatctcact
       61 taaggctttc ttataaatct gtagggtttt gcctggaagc agacaaataa cccgataaaa
      121 caaggatgag cagatgagcg gaaaaccggc agcgcgtcag ggcgacatga cgcagtatgg
      181 cggtagcatt gttcagggtt cagccggggt gcgcatcggt gcccccaccg gtgtggcctg
      241 ttcg
//
LOCUS       DAZRVY010000485          242 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZRVY010000485 DAZRVY010000000
VERSION     DAZRVY010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.488"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHXOX_004731"
     CDS             <1..220
                     /locus_tag="ACHXOX_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7007956.1"
                     /translation="RKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEI
                     FYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT           75 a           52 c           58 g           57 t
ORIGIN      
        1 gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa
       61 tgagagcctg agccactatc gttttaataa ccgggatgaa gccatctcag taatacggga
      121 atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga atatctcccc
      181 ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaaca aatggtagtg
      241 tc
//
LOCUS       DAZRVY010000486          242 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZRVY010000486 DAZRVY010000000
VERSION     DAZRVY010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.489"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>242
                     /locus_tag="ACHXOX_004732"
     CDS             <1..>242
                     /locus_tag="ACHXOX_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7007957.1"
                     /translation="LFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQLV
                     AAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLD"
BASE COUNT           59 a           74 c           53 g           56 t
ORIGIN      
        1 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
       61 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
      121 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
      181 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
      241 cc
//
LOCUS       DAZRVY010000487          240 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZRVY010000487 DAZRVY010000000
VERSION     DAZRVY010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.490"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHXOX_004733"
     rRNA            <1..>240
                     /locus_tag="ACHXOX_004733"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           55 c           80 g           44 t
ORIGIN      
        1 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
       61 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      121 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      181 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZRVY010000488          238 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZRVY010000488 DAZRVY010000000
VERSION     DAZRVY010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.491"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            71..146
                     /locus_tag="ACHXOX_004734"
     tRNA            71..146
                     /locus_tag="ACHXOX_004734"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           59 a           57 c           59 g           63 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctgga
//
LOCUS       DAZRVY010000489          236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZRVY010000489 DAZRVY010000000
VERSION     DAZRVY010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.492"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            52..>236
                     /locus_tag="ACHXOX_004735"
     CDS             52..>236
                     /locus_tag="ACHXOX_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7007958.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITP"
BASE COUNT           79 a           59 c           54 g           44 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacacc
//
LOCUS       DAZRVY010000490          231 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZRVY010000490 DAZRVY010000000
VERSION     DAZRVY010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.493"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..220)
                     /locus_tag="ACHXOX_004736"
     CDS             complement(<1..220)
                     /locus_tag="ACHXOX_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR7007959.1"
                     /translation="MATTCSVILILESFDVYFGKESVFLERGSSVLVDSSSRDFFLTY
                     PERVIVADFGAEFISRYLKANNLRDISDC"
BASE COUNT           86 a           59 c           29 g           57 t
ORIGIN      
        1 tacaatcaga aatatccctt aagttattag ctttcaaata gcgactaata aactcagcgc
       61 caaaatccgc cactatcact ctttcaggat atgtcaggaa aaaatctcta gagctagagt
      121 cgacaagtac agatgaacct ctctccagaa acacactctc ttttccgaaa taaacatcaa
      181 aggactccaa aatcaatata actgaacatg tagttgccat atcatccacc c
//
LOCUS       DAZRVY010000491          225 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZRVY010000491 DAZRVY010000000
VERSION     DAZRVY010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.494"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            1..225
                     /locus_tag="ACHXOX_004737"
                     /pseudo
     CDS             1..225
                     /locus_tag="ACHXOX_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           58 a           59 c           64 g           44 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccg
//
LOCUS       DAZRVY010000492          223 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZRVY010000492 DAZRVY010000000
VERSION     DAZRVY010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.495"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..122)
                     /locus_tag="ACHXOX_004738"
     CDS             complement(<1..122)
                     /locus_tag="ACHXOX_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7007960.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRA"
     gene            complement(137..>223)
                     /locus_tag="ACHXOX_004739"
     CDS             complement(137..>223)
                     /locus_tag="ACHXOX_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR7007961.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
BASE COUNT           53 a           73 c           43 g           54 t
ORIGIN      
        1 gcacgactga tacctgtttc atctgacaac tgccgaatcg tgacccgacc gtcctgatgc
       61 acgagttcca caattcgcgt cttcagttct tcccgctgtt cgggagtaaa aggtttcgcc
      121 ataaatcctc ctgaaactac ttaacaaccc tcgaatggct aacattcgga cgccagctct
      181 cccagttaaa attcacccag cgcccgccgt tcatgaccat gcg
//
LOCUS       DAZRVY010000493          222 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZRVY010000493 DAZRVY010000000
VERSION     DAZRVY010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.496"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            <1..>222
                     /locus_tag="ACHXOX_004740"
     CDS             <1..>222
                     /locus_tag="ACHXOX_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR7007962.1"
                     /translation="WVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGK
                     ALFMALRSQRPPAGLIHHSDRGSQYCAYD"
BASE COUNT           52 a           57 c           65 g           48 t
ORIGIN      
        1 tctgggtggc ggacctgacg tatgttgcca cacaggaggg atggttgtac ctcgctggca
       61 tcaaagatgt ttatacgtgc gaaattgtcg gctacgccat gggagagcgc atgacaaaag
      121 agctgacagg taaagccctg tttatggcgc tcaggagcca gcgcccacct gccgggctaa
      181 tccaccactc tgatcgaggt tcacagtact gcgcatacga tt
//
LOCUS       DAZRVY010000494          210 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZRVY010000494 DAZRVY010000000
VERSION     DAZRVY010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.497"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT           84 a           38 c           19 g           69 t
ORIGIN      
        1 tattcagcaa taacttaata atatatatct ggataaccat acccaactgt tcaaagtccg
       61 tattgcctcc ataaaatggc caaagctgaa tcatgaataa atattgtatt tatcctttct
      121 gaattatcat tttgataatt tttgagatat tccttaaaaa tacccactta atcaatcaca
      181 aaacaaatat ataaacaagc aatcattaga
//
LOCUS       DAZRVY010000495          204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZRVY010000495 DAZRVY010000000
VERSION     DAZRVY010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.498"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
BASE COUNT           87 a           37 c           22 g           58 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac atta
//
LOCUS       DAZRVY010000496          202 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZRVY010000496 DAZRVY010000000
VERSION     DAZRVY010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.denovo.499"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..>202)
                     /locus_tag="ACHXOX_004741"
     CDS             complement(<1..>202)
                     /locus_tag="ACHXOX_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFR7007963.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRL"
BASE COUNT           26 a           85 c           44 g           47 t
ORIGIN      
        1 ttcagcctcc ccgcctcatc atactcgctg cgggtgatgc tcccgtcggc atgctccttc
       61 ttcaccaccc gcttcaggcc accctcgcct tccgtgtaca gcacctcccg ccggttcagg
      121 ctgtccgtga tgattacccg gctctctccg tactcaaagc ggtagtccag cccctccggg
      181 ttgacctgct ccgtcacccg ac
//
LOCUS       DAZRVY010000497         6807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.guided.179, whole genome shotgun sequence.
ACCESSION   DAZRVY010000497 DAZRVY010000000
VERSION     DAZRVY010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.guided.179"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(371..520)
                     /locus_tag="ACHXOX_004742"
     CDS             complement(371..520)
                     /locus_tag="ACHXOX_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007964.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1091..1249
                     /locus_tag="ACHXOX_004743"
     CDS             1091..1249
                     /locus_tag="ACHXOX_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7007965.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            1709..2524
                     /gene="sul2"
                     /locus_tag="ACHXOX_004744"
     CDS             1709..2524
                     /gene="sul2"
                     /locus_tag="ACHXOX_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR7007966.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2585..3112
                     /locus_tag="ACHXOX_004745"
                     /pseudo
     CDS             2585..3112
                     /locus_tag="ACHXOX_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            3133..3606
                     /gene="dfrA14"
                     /locus_tag="ACHXOX_004746"
     CDS             3133..3606
                     /gene="dfrA14"
                     /locus_tag="ACHXOX_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HFR7007967.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            3681..3956
                     /locus_tag="ACHXOX_004747"
                     /pseudo
     CDS             3681..3956
                     /locus_tag="ACHXOX_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            3956..4792
                     /locus_tag="ACHXOX_004748"
     CDS             3956..4792
                     /locus_tag="ACHXOX_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR7007968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4899..5303)
                     /locus_tag="ACHXOX_004749"
                     /pseudo
     CDS             complement(4899..5303)
                     /locus_tag="ACHXOX_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(5347..5736)
                     /locus_tag="ACHXOX_004750"
     CDS             complement(5347..5736)
                     /locus_tag="ACHXOX_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007969.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            5954..6394
                     /locus_tag="ACHXOX_004751"
     CDS             5954..6394
                     /locus_tag="ACHXOX_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR7007970.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6453..>6807)
                     /locus_tag="ACHXOX_004752"
     CDS             complement(6453..>6807)
                     /locus_tag="ACHXOX_004752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7007971.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT         1683 a         1771 c         1763 g         1590 t
ORIGIN      
        1 gatgtaaaag tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg agcgatgtaa
       61 ttctgtagtc aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct
      121 ttttttgtca cgatctcccc ttaacctgcg taaatcaccc gccatggttg attatttttc
      181 aacctcaaaa tggtcataca cctaacgcag ccgcgatttt catcgcaact gcatgaatta
      241 aaagaaaaaa aaggaatatt tcgttttcac gagtaatacc cgggtattaa aaatccactg
      301 taattacatg aaaataaaga aataaaaggt aataccggag tattaccacg tacgcgggca
      361 agcgaaaaag ctataacgct tcccggccga caggcgaccc aaaatccaca ggggcatgca
      421 ggttctcttc agtaacctgc gccattaaat caaaaaggtc atattcgccg ggaagctgcg
      481 tatcagtcga atccagttgt tcatcatatt cagatggcat ggggtgatat ccagtagctg
      541 agaggttgtt agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg ctagcggagt
      601 gtatactggc ttactatgtt gccacgattg cgaaaatcag tgaagtgctt catgtggcag
      661 gagaaaaaag gcagcaccgg cgcgtcagca gaatatgcga tacaggagat attccgcttc
      721 ctcgctcact gactcgctac gctcggtcgt tcggctgcgg cgagcggtac cggcttacgg
      781 acggggacgg aaatttcctg gaagatacca ggaagacact tagcaaagaa gagataaggc
      841 cacggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcgca aaatctgacg
      901 ctcaaatcag tggtggcgaa acccgacagg actataaaga tcccaggcgt ttccccctgg
      961 tagctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1021 atggccgcgg tttctcattc cacgcctgac actcagttcc gtgaaggcag ttcgctccaa
     1081 gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttgt ccggtaacta
     1141 tcaacttgag tccaacccgg aaagacacga caaaacgcca ctggcagcag ccattagtaa
     1201 caggagtgca cgagaagata gattaagagg tcttgaagta gaggcctgac tgcggctaca
     1261 ctgaaagaac agttttggta atggcggtct cgtgaaccct gttaccgggt taagcagttc
     1321 cccaactgac ttaaccttcg aacaaaccgc ctccccaggc ggttttttcg tttacagagc
     1381 aagagattac gattcattga aaaggatctc aagaagatcc tttgtagatt actatcaatt
     1441 aatcttccga acatgacacc agtcactgtt gattacggtt aaaccgctcc gtaacaggtt
     1501 tccctcgtat tggtgacttg cagcaccaaa gaagcaaaaa agataaatat atctacttat
     1561 tgagagatga tatatttatt tccaaaatgc tacagataca tcatcatgaa acggatcacc
     1621 gcaaacaggt tactcgcctg gggattccct ttcgacccga gcatccgtat gagactcatg
     1681 ctcgattatt attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt
     1741 caacataacc tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat
     1801 tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc
     1861 cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt
     1921 gctggacgcg ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac
     1981 gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga
     2041 cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt
     2101 gcaagacggg caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc
     2161 ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct
     2221 tgtccttgat cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt
     2281 gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc
     2341 gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac
     2401 actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc
     2461 gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg
     2521 ttaactgcac attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc
     2581 tccattgaat cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag
     2641 aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat
     2701 cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa
     2761 aggtcgagat gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg
     2821 cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct
     2881 caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca
     2941 atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg
     3001 caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct
     3061 tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgttaaccc
     3121 aggatgagaa ccttgaaagt atcattgata gctgcgaaag cgaaaaacgg cgtgattggt
     3181 tgcggtccag acataccctg gtccgcgaaa ggggagcagc tactttttaa agcattgacc
     3241 tacaatcagt ggcttctggt gggtcgcaag acgtttgaat ctatgggcgc actccccaat
     3301 aggaaatacg cggtcgttac ccgctcaggt tggacatcaa atgatgacaa tgtagttgta
     3361 tttcagtcaa tcgaagaggc catggacagg ctagctgaat tcaccggtca cgttatagtg
     3421 tctggtggcg gagaaattta ccgagaaaca ttacccatgg cctctacgct ccacttatcg
     3481 acgatcgaca tcgagccaga gggggatgtt ttcttcccga gtattccaaa taccttcgaa
     3541 gttgtttttg agcaacactt tacttcaaac attaactatt gctatcaaat ttggaaaaag
     3601 ggttaacaaa gctatgcaat cgacggcaaa aagcttcgtt cgcttcgcgc actacgcctt
     3661 tttccgcgat tgatagcgac gatatggttg tttgccatgg tgatccctgc atgccgaact
     3721 tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa
     3781 cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag
     3841 cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc
     3901 ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt
     3961 catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac
     4021 cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg
     4081 attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg
     4141 caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata
     4201 tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga
     4261 aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc
     4321 ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa
     4381 cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa
     4441 tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag
     4501 tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc
     4561 caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc
     4621 acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca
     4681 ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg
     4741 cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact agatatcaag
     4801 cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc tggccaaagc
     4861 cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg cagctgaaac
     4921 gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc aaccagcaag
     4981 gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg acgcttacag
     5041 ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc
     5101 tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc
     5161 tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag
     5221 agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc
     5281 gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc
     5341 tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg
     5401 ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt acatgttatt
     5461 gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga cagaattaat
     5521 attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa aggatgcagt
     5581 ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa aactacctcc
     5641 cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca tgtgaccaaa
     5701 accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt tgacttttaa
     5761 aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa atactacgcc
     5821 cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac gggcagcctc
     5881 agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac acacaggaga
     5941 cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa caggaaagtc
     6001 gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca caggaaaaaa
     6061 tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc tgcgcacttc
     6121 tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc aggcggaaaa
     6181 tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg ttaccctaat
     6241 gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg agagggaaca
     6301 actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa agcggggatt
     6361 ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct gatttaccat
     6421 aacatctgtt atcagcaccg cctgagaggc cctcagggcg tttctgacgg gaaaccggta
     6481 aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca
     6541 tgcatgagta tgcgcacaag tgcatagcgc ggaaattcac accattcagc cggggtaagt
     6601 cctgtttccg gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg
     6661 ccgtgaagcg ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg
     6721 atgtacaaat ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg
     6781 atgtataagt gatgtaaaag tgatgta
//
LOCUS       DAZRVY010000498         6377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.guided.186, whole genome shotgun sequence.
ACCESSION   DAZRVY010000498 DAZRVY010000000
VERSION     DAZRVY010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.guided.186"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHXOX_004753"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHXOX_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHXOX_004754"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHXOX_004754"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR7007972.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHXOX_004755"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHXOX_004755"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR7007973.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHXOX_004756"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHXOX_004756"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR7007974.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHXOX_004757"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHXOX_004757"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR7007975.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHXOX_004758"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHXOX_004758"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR7007976.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHXOX_004759"
     CDS             5099..6232
                     /locus_tag="ACHXOX_004759"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR7007977.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT         1613 a         1653 c         1644 g         1467 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgc
//
LOCUS       DAZRVY010000499         5059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1706196
            SAMN43277073-rid23491653.guided.213, whole genome shotgun sequence.
ACCESSION   DAZRVY010000499 DAZRVY010000000
VERSION     DAZRVY010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277073
            Sequence Read Archive: SRR30421622
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396282.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396282.1
            Genome Coverage       :: 87x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 18:27:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,765
            CDSs (total)                      :: 4,676
            Genes (coding)                    :: 4,390
            CDSs (with protein)               :: 4,390
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 130 of 286
            Pseudo Genes (incomplete)         :: 99 of 286
            Pseudo Genes (internal stop)      :: 86 of 286
            Pseudo Genes (multiple problems)  :: 28 of 286
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277073-rid23491653.guided.213"
                     /strain="M1706196"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2017-07-13"
                     /collected_by="FSS"
     gene            complement(<1..766)
                     /gene="pepD"
                     /locus_tag="ACHXOX_004760"
     CDS             complement(<1..766)
                     /gene="pepD"
                     /locus_tag="ACHXOX_004760"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HFR7007978.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLR"
     gene            1027..1485
                     /gene="gpt"
                     /locus_tag="ACHXOX_004761"
     CDS             1027..1485
                     /gene="gpt"
                     /locus_tag="ACHXOX_004761"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR7007979.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1577..2821
                     /gene="frsA"
                     /locus_tag="ACHXOX_004762"
     CDS             1577..2821
                     /gene="frsA"
                     /locus_tag="ACHXOX_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR7007980.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2879..3280
                     /gene="crl"
                     /locus_tag="ACHXOX_004763"
     CDS             2879..3280
                     /gene="crl"
                     /locus_tag="ACHXOX_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR7007981.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3319..4374)
                     /gene="phoE"
                     /locus_tag="ACHXOX_004764"
     CDS             complement(3319..4374)
                     /gene="phoE"
                     /locus_tag="ACHXOX_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR7007982.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            4662..>5059
                     /locus_tag="ACHXOX_004765"
     CDS             4662..>5059
                     /locus_tag="ACHXOX_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR7007983.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPV"
BASE COUNT         1243 a         1250 c         1251 g         1315 t
ORIGIN      
        1 tacgcagtgt gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg
       61 ctaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg
      121 agtgaccccc tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg
      181 cttcacgatc taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga
      241 tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct
      301 gtaagccgaa cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg
      361 ggccgtgaac cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt
      421 tatctgcacc cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga
      481 taggatcttt cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt
      541 gggcctgtaa gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca
      601 ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
      661 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
      721 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
      781 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
      841 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
      901 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
      961 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     1021 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     1081 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     1141 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     1201 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa
     1261 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     1321 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     1381 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     1441 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     1501 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     1561 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     1621 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     1681 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     1741 gtggatctgg cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat
     1801 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg
     1861 caactggatt tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga
     1921 agaagatccg caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc
     1981 tgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg
     2041 tgcctatgaa gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     2101 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     2161 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     2221 acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt
     2281 ttcatcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc
     2341 tctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     2401 cgctaacgtt gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg
     2461 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga
     2521 aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt
     2581 acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc
     2641 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     2701 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta
     2761 tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta
     2821 aaaatttgct aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat
     2881 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     2941 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     3001 cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga
     3061 agcgcaggaa tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga
     3121 ctggaagagt gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg
     3181 tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc
     3241 ggatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat
     3301 cacatccggc aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt
     3361 attaatattc aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga
     3421 catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc
     3481 accgatacct tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc
     3541 aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc
     3601 agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc
     3661 atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag
     3721 gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc
     3781 gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg
     3841 gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa
     3901 gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg
     3961 cgccgaggaa tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa
     4021 cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa
     4081 agcgagacgc gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc
     4141 ttcccaccga ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa
     4201 acggatataa ctctggtcgc catctttact ggcgttatca ctcatataat gcatggcttt
     4261 aactttgcca tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac
     4321 agatgcagat gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat
     4381 tcctgatttt aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt
     4441 ttttaacctg agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa
     4501 tttattaaat ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa
     4561 caaaacgcca tgctttgctc acagatggtt ggcaaccgac gacagtcctg ctaaaacgtt
     4621 cgtttgatat catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc
     4681 tggtggtaaa actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc
     4741 atattgttga acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta
     4801 ttgtgacgtc gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag
     4861 cgactatcgc ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt
     4921 gggaacagct gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg
     4981 atatggaaga ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata
     5041 acaatatcgt tccggtaat
//
